Clone ID: APC115038.106 (APC115038.26-783.P(785).E536A.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVTGDITTGQAKSSDVAQKIEKGLVGGLFNLKGVDRILEVQKLAVEKSRLGIPLLFGMDVIHGYETIFPIPLGLSCTWDMAAIEKSARIAAIEASADGISWTFSPMVDISRDPRWGRVSEGSGEDPFLGGAIAQAMVYGYQGANLQDQLHRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEFMYPYEAAVEAGVGSVMASFNEIDGIPATGNKWLLSDLLRGQWGFEGFVVTDFTGISEMIEHGVGDLQTVSALALNAGVDMDMVSEGFVGTLMKSIKEGKVRMGTLNTACRRILEAKYKLGLFDNPYKYCDVNRPKRDIFTKEHRDAARKIAGESFVLLKNAPATAQPLAAHSSSPVTASPVLPLKKQGTVAVIGPLGNTRSNMPGTWSVAARLNDYPSLYEGLKEMMAGKVNITYAKGSNLIGDAAYEERATMFGRSLNRDNRTDQELLDEALKIAAGADVIVAALGESSEMSGESSSRTELGLPDVQHTLLEALLKTGKPVVLTLFTGRPLTLNWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLAAGKWFEKFRSNYLDVDNEPLYPFGYGLSYTTFQYSDIALSTPTLGKDGSVTAVVTVTNTGKHDGAEVVQLYIRDLVGSITRPVRELKGFNKIFLRAGESKTVSFTITRDLLRFYDYDLNYVAEPGDFDIMIGGNSQAVKTAKLTL
AAGTCACCGCAAGACATGGATCGCTTCATCGACGCACTGATGAAGAAGATGACCGTGGAAGAGAAAATCGGACAATTGAACCTACCCGTCACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCACAAAAGATTGAAAAAGGATTGGTGGGCGGACTCTTCAACCTAAAAGGTGTAGACCGTATTCTTGAAGTGCAAAAGCTGGCAGTAGAGAAATCACGCCTCGGTATTCCCCTGCTGTTCGGCATGGATGTGATACATGGCTACGAAACCATCTTCCCCATTCCATTGGGATTGTCCTGCACCTGGGATATGGCGGCTATCGAGAAATCCGCCCGTATTGCAGCCATCGAAGCAAGTGCCGATGGCATTTCCTGGACATTCAGTCCGATGGTAGACATCAGTCGCGACCCACGTTGGGGACGTGTCAGCGAGGGCTCGGGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTATACGGATACCAGGGTGCCAATCTGCAAGACCAATTGCACCGCAACGATGAAATCATGGCTTGCGTAAAACACTTTGCATTGTATGGAGCCGGAGAAGCCGGACGCGACTATAATACAGTAGATATGAGCCGCAACCGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAGGCCGGAGTGGGTAGTGTGATGGCGTCATTCAATGAAATAGACGGTATTCCCGCCACCGGAAACAAATGGCTGCTGAGCGATTTGCTGCGTGGCCAGTGGGGCTTCGAAGGGTTTGTGGTAACGGACTTCACAGGCATTTCAGAGATGATAGAGCATGGTGTCGGCGACTTGCAAACCGTCAGTGCACTCGCTCTTAATGCAGGGGTGGACATGGATATGGTAAGTGAGGGCTTCGTCGGTACACTGATGAAATCAATTAAAGAAGGAAAAGTAAGAATGGGCACGTTGAATACAGCCTGCCGCCGGATATTGGAAGCGAAATACAAGCTGGGACTGTTTGACAATCCTTATAAATACTGCGACGTGAACCGTCCGAAGCGGGATATCTTCACAAAAGAGCATCGTGACGCCGCCCGCAAGATTGCCGGCGAAAGTTTTGTTCTTCTGAAGAATGCCCCCGCCACCGCACAGCCACTCGCAGCTCATAGCTCGTCACCCGTAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAAGGTACAGTTGCCGTCATCGGCCCTCTCGGAAATACCCGCAGCAACATGCCGGGCACCTGGAGCGTAGCCGCACGCCTCAACGATTATCCTTCTTTATACGAAGGCTTGAAAGAAATGATGGCAGGCAAGGTGAACATCACCTATGCCAAAGGTAGTAACCTCATCGGCGATGCAGCTTACGAAGAACGTGCCACCATGTTCGGCCGTTCATTGAACCGCGATAATCGCACGGACCAGGAGTTACTGGACGAAGCACTGAAAATTGCAGCCGGCGCCGATGTTATCGTAGCTGCCCTGGGAGAATCTTCTGAAATGAGCGGTGAAAGTTCAAGCCGCACCGAACTCGGCTTGCCCGATGTACAACATACCCTGTTGGAAGCCTTACTGAAAACGGGTAAACCCGTAGTACTAACCCTCTTTACCGGTCGGCCGTTGACGCTGAACTGGGAACAGGAGCATGTACCTGCCATCTTGAATGTATGGTTCGGAGGCAGTGAAGCGGCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTCAATCCGAGTGGAAAACTAACCATGACTTTCCCGAAGAATGTAGGCCAGATACCTTTGTTCTACAATCATAAGAATACCGGTCGGCCACTGGCGGCAGGCAAATGGTTCGAAAAGTTCCGTTCAAACTATCTGGATGTGGATAACGAACCGCTGTATCCCTTCGGTTATGGATTGTCGTATACCACTTTCCAGTACAGTGACATTGCATTGAGCACACCGACATTGGGAAAAGATGGTTCCGTTACAGCCGTAGTCACCGTCACCAATACTGGTAAACATGACGGTGCGGAAGTAGTTCAACTCTATATCCGCGACCTCGTAGGAAGTATCACCCGCCCTGTACGCGAGTTGAAAGGTTTCAATAAAATCTTCCTTCGCGCCGGAGAAAGCAAAACGGTATCATTCACTATCACGCGTGATCTGCTTCGCTTCTATGATTACGACCTGAACTACGTAGCCGAACCGGGTGACTTTGACATCATGATCGGTGGAAACAGCCAGGCTGTGAAGACGGCGAAGTTGACACTT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,697 total letters Query= APC115038.106 Length=2679 Score E Sequences producing significant alignments: (Bits) Value wt 4093 0.0 07A_A1_APC115038.106_A01.seq 1139 0.0 01A_A1_APC115038.106_A01.seq 262 1e-72 K S P Q D M D R F I D A L M K K M T V E E K I G Q L N L P V Query_1 202 AAGTCACCGCAAGACATGGATCGCTTCATCGACGCACTGATGAAGAAGATGACCGTGGAAGAGAAAATCGGACAATTGAACCTACCCGTC 291 2 1 .......................................................................................... 90 T G D I T T G Q A K S S D V A Q K I E K G L V G G L F N L K Query_1 292 ACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCACAAAAGATTGAAAAAGGATTGGTGGGCGGACTCTTCAACCTAAAA 381 2 91 .......................................................................................... 180 G V D R I L E V Q K L A V E K S R L G I P L L F G M D V I H Query_1 382 GGTGTAGACCGTATTCTTGAAGTGCAAAAGCTGGCAGTAGAGAAATCACGCCTCGGTATTCCCCTGCTGTTCGGCATGGATGTGATACAT 471 2 181 .......................................................................................... 270 G Y E T I F P I P L G L S C T W D M A A I E K S A R I A A I Query_1 472 GGCTACGAAACCATCTTCCCCATTCCATTGGGATTGTCCTGCACCTGGGATATGGCGGCTATCGAGAAATCCGCCCGTATTGCAGCCATC 561 2 271 .......................................................................................... 360 E A S A D G I S W T F S P M V D I S R D P R W G R V S E G S Query_1 562 GAAGCAAGTGCCGATGGCATTTCCTGGACATTCAGTCCGATGGTAGACATCAGTCGCGACCCACGTTGGGGACGTGTCAGCGAGGGCTCG 651 2 361 .......................................................................................... 450 G E D P F L G G A I A Q A M V Y G Y Q G A N L Q D Q L H R N Query_1 652 GGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTATACGGATACCAGGGTGCCAATCTGCAAGACCAATTGCACCGCAAC 741 2 451 .......................................................................................... 540 D E I M A C V K H F A L Y G A G E A G R D Y N T V D M S R N Query_1 742 GATGAAATCATGGCTTGCGTAAAACACTTTGCATTGTATGGAGCCGGAGAAGCCGGACGCGACTATAATACAGTAGATATGAGCCGCAAC 831 2 541 .......................................................................................... 630 R M F N E F M Y P Y E A A V E A G V G S V M A S F N E I D G Query_1 832 CGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAGGCCGGAGTGGGTAGTGTGATGGCGTCATTCAATGAAATAGACGGT 921 2 631 .......................................................................................... 720 I P A T G N K W L L S D L L R G Q W G F E G F V V T D F T G Query_1 922 ATTCCCGCCACCGGAAACAAATGGCTGCTGAGCGATTTGCTGCGTGGCCAGTGGGGCTTCGAAGGGTTTGTGGTAACGGACTTCACAGGC 1011 2 721 .......................................................................................... 810 I S E M I E H G V G D L Q T V S A L A L N A G V D M D Query_1 1012 ATTTCAGAGATGATAGAGCATGGTGTCGGC--GACTTGC-AAACCGTCAGTGCACTCGC--TCTTAAT-GCAGGG-GTGGA-CATGG-AT 1092 2 811 ..............................--.......-...................--.......-......-.....-.....-.. 891 1 812 ....N......NAGN.....GT........N.....N...NCT.......N......T.....T.....G.. 741 M V S E G F V G T L M K S I K E G K V R M G T L N T A Query_1 1093 ATGG-TAAGTGAGGGC--TTCGTCGGTA-CACTG-ATGAAATCAATTAAAGAAGGAAAAGTAAGAAT--GGGCACGTTGAATACA-GCCT 1174 2 892 ....-...........--..........-.....-................................--................-.... 973 1 740 ....N..N....AN.ANT.....N..G.A.....G.N.N.T..N...N......N...........ATG....N...G.......C.... 651 C R R I L E A K Y K L G L F D N P Y K Y C D V N R P K R D Query_1 1175 GCCGCCGGATATTGGAAGCGAAATACAAGC-TGGG-ACTGTTTGACAATCCTTATAAATACTGCGACGTGAACCGTCCGAAGCGGGATAT 1262 2 974 ..............................-....-...................................................... 1061 1 650 ..N............N........N....NA....N.N......N...............N...............T...........N. 561 I F T K E H R D A A R K I A G E S F V L L K N A P A T A Q P Query_1 1263 CTTCACAAAAGAGCATCGTGACGCCGCCCGCAAGATTGCCGGCGAAAGTTTTGTTCTTCTGAAGAATGCCCCCGCCACCGCACAGCCACT 1352 2 1062 .......................................................................................... 1151 1 560 N...............N......................................................................... 471 L A A H S S S P V T A S P V L P L K K Q G T V A V I G P L G Query_1 1353 CGCAGCTCATAGCTCGTCACCCGTAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAAGGTACAGTTGCCGTCATCGGCCCTCTCGGAAA 1442 2 1152 .......................................................................................... 1241 1 470 ..........................N......................N........................................ 381 N T R S N M P G T W S V A A R L N D Y P S L Y E G L K E M M Query_1 1443 TACCCGCAGCAACATGCCGGGCACCTGGAGCGTAGCCGCACGCCTCAACGATTATCCTTCTTTATACGAAGGCTTGAAAGAAATGATGGC 1532 2 1242 .......................................................................................... 1331 1 380 .........................................................................N................ 291 A G K V N I T Y A K G S N L I G D A A Y E E R A T M F G R S Query_1 1533 AGGCAAGGTGAACATCACCTATGCCAAAGGTAGTAACCTCATCGGCGATGCAGCTTACGAAGAACGTGCCACCATGTTCGGCCGTTCATT 1622 2 1332 .......................................................................................... 1421 1 290 .......................................................................................... 201 L N R D N R T D Q E L L D E A L K I A A G A D V I V A A L G Query_1 1623 GAACCGCGATAATCGCACGGACCAGGAGTTACTGGACGAAGCACTGAAAATTGCAGCCGGCGCCGATGTTATCGTAGCTGCCCTGGGAGA 1712 2 1422 .......................................................................................... 1511 1 200 .......................................................................................... 111 0 292 ...............NN......NCAANN.AN..N...GNN..CNNC...A....TTCCN 351 E S S E M S G A S S S R T E L G L P D V Q H T L L E A L L Query_1 1713 ATCTTCTGAAATGA-GCGGTGCTAGTTCAAGCCGCACCGAACTCGGCTTGCCCGATGTACAA-CAT-ACCCTGTTGGAAGCCTTACTGAA 1799 2 1512 ..............-......AA.......................................-...-....................... 1598 1 110 ..............N...............................................G...A....................... 21 0 352 G..AA.NNT.....-.......N.ACG-..C..NNNN.........T......N...NN...-NN.-N..........C-.......... 436 K T G K P V V L T L F T G R P L T L N W E Q E H V P A I L N Query_1 1800 AA-CGGGTAAACCCGTAGTACTAACCCTCTTTACCGGTCGGCCGTTGACGCTGAACTGGGAACAGGAGCATGTACCTGCCATCTTGAATG 1888 2 1599 ..-....................................................................................... 1687 2 1821 ............ 1832 1 20 ..N...... 12 0 437 ..-........TN..N.CA.........N.....T....T.AGN.C.GACT...-.......NNN...NNC.C........N........ 524 V W F G G S E A A Y A I G D V L F G D V N P S G K L T M T F Query_1 1889 TATGGTTCGGAGGCAGTGAAGCGGCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTCAATCCGAGTGGAAAACTAACCATGACTTTCC 1978 2 1688 .......................................................................................... 1777 0 525 .N...N.N.N.A.NNNN.N.NN.AT........N..N.A.....NN.N....N...N.N....NNN....NMN.N..N......N...N. 614 P K N V G Q I P L F Y N H K N T G R P L A A G K W F E K F R Query_1 1979 CGAAGAATGTAGGCCAGATACCTTTGTTCTACAATCATAAGAATACCGGTC-GGCCACTGGCGGCAGGCAAATGGTTCGAAAAGTTCCGT 2067 2 1778 ...................................................-...................................... 1866 2 1629 ........-.... 1640 0 615 N.G......N.T....C...NNN...N.....N..N....N..N.N.N...N........N..N.....-....NG..NNNC..G....A 703 S N Y L D V D N E P L Y P F G Y G L S Y T T F Q Y S D I A L Query_1 2068 TCAAACTATCTGGATGTGGATAACGAACCGCTGTATCCCTTCGGTTATGGATTGTCGTATACCACTTTCCAGTACAGTGACATTGCATTG 2157 2 1867 .......................................................................................... 1956 0 704 CNN.........T.....C.N...-NN...G..CN..N....CNNN..A..G..C.NC...N...N......AN.......G..N...C. 792 S T P T L G K D G S V T A V V T V T N T G K H D G A E V V Query_1 2158 AGCACACCGACATTGGGAAAAGAT-GGTTCCGTTACAGCCGTAGTCACCGTCACCAATACTGGTAAACATGACGGT-GCGGAAGTAGTTC 2245 2 1957 ........................-...................................................-............. 2044 0 793 .NTNA...NNNNN...NN..NA..G..NN....N..NNN.......W...ANC..G..TANN.CT........A..CA..A....G.C.. 882 Q L Y I R D L V G S I T R P V R E L K G F N K I F L R A G Query_1 2246 AAC-TCTATATCCGCGACCTCGTAGGAAGTATCACCCGCCCTGTAC-GCGAGTTGAAAGGTTTCAATAAAATCTTCCTTCGCGCCGGAGA 2333 2 2045 ...-..........................................-........................................... 2132 0 883 .T.NN..N....N..NGN.GNC.NA.N.NN.CN...A.T.TN....N.........N....C...TN... 952 E S K T V S F T I T R D L L R F Y D Y D L N Y V A E P G D F Query_1 2334 AAGCAAAACGGTATCATTCACTATCACGCGTGATCTGCTTCGCTTCTATGATTACGACCTGAACTACGTAGCCGAACCGGGTGACTTTGA 2423 2 2133 .......................................................................................... 2222 D I M I G G N S Q A V K T A K L T L Query_1 2424 CATCATGATCGGTGGAAACAGCCAGGCTGTGAAGACGGCGAAGTTGACACTT 2475 2 2223 .................................................... 2274 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12367652 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:06 PM Number of letters in database: 4,697 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115038.107 (APC115038.26-783.P(785).W448A.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVTGDITTGQAKSSDVAQKIEKGLVGGLFNLKGVDRILEVQKLAVEKSRLGIPLLFGMDVIHGYETIFPIPLGLSCTWDMAAIEKSARIAAIEASADGISWTFSPMVDISRDPRWGRVSEGSGEDPFLGGAIAQAMVYGYQGANLQDQLHRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEFMYPYEAAVEAGVGSVMASFNEIDGIPATGNKWLLSDLLRGQWGFEGFVVTDFTGISEMIEHGVGDLQTVSALALNAGVDMDMVSEGFVGTLMKSIKEGKVRMGTLNTACRRILEAKYKLGLFDNPYKYCDVNRPKRDIFTKEHRDAARKIAGESFVLLKNAPATAQPLAAHSSSPVTASPVLPLKKQGTVAVIGPLGNTRSNMPGTWSVAARLNDYPSLYEGLKEMMAGKVNITYAKGSNLIGDAAYEERATMFGRSLNRDNRTDQELLDEALKIAAGADVIVAALGESSEMSGESSSRTELGLPDVQHTLLEALLKTGKPVVLTLFTGRPLTLNWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLAAGKWFEKFRSNYLDVDNEPLYPFGYGLSYTTFQYSDIALSTPTLGKDGSVTAVVTVTNTGKHDGAEVVQLYIRDLVGSITRPVRELKGFNKIFLRAGESKTVSFTITRDLLRFYDYDLNYVAEPGDFDIMIGGNSQAVKTAKLTL
AAGTCACCGCAAGACATGGATCGCTTCATCGACGCACTGATGAAGAAGATGACCGTGGAAGAGAAAATCGGACAATTGAACCTACCCGTCACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCACAAAAGATTGAAAAAGGATTGGTGGGCGGACTCTTCAACCTAAAAGGTGTAGACCGTATTCTTGAAGTGCAAAAGCTGGCAGTAGAGAAATCACGCCTCGGTATTCCCCTGCTGTTCGGCATGGATGTGATACATGGCTACGAAACCATCTTCCCCATTCCATTGGGATTGTCCTGCACCTGGGATATGGCGGCTATCGAGAAATCCGCCCGTATTGCAGCCATCGAAGCAAGTGCCGATGGCATTTCCTGGACATTCAGTCCGATGGTAGACATCAGTCGCGACCCACGTTGGGGACGTGTCAGCGAGGGCTCGGGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTATACGGATACCAGGGTGCCAATCTGCAAGACCAATTGCACCGCAACGATGAAATCATGGCTTGCGTAAAACACTTTGCATTGTATGGAGCCGGAGAAGCCGGACGCGACTATAATACAGTAGATATGAGCCGCAACCGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAGGCCGGAGTGGGTAGTGTGATGGCGTCATTCAATGAAATAGACGGTATTCCCGCCACCGGAAACAAATGGCTGCTGAGCGATTTGCTGCGTGGCCAGTGGGGCTTCGAAGGGTTTGTGGTAACGGACTTCACAGGCATTTCAGAGATGATAGAGCATGGTGTCGGCGACTTGCAAACCGTCAGTGCACTCGCTCTTAATGCAGGGGTGGACATGGATATGGTAAGTGAGGGCTTCGTCGGTACACTGATGAAATCAATTAAAGAAGGAAAAGTAAGAATGGGCACGTTGAATACAGCCTGCCGCCGGATATTGGAAGCGAAATACAAGCTGGGACTGTTTGACAATCCTTATAAATACTGCGACGTGAACCGTCCGAAGCGGGATATCTTCACAAAAGAGCATCGTGACGCCGCCCGCAAGATTGCCGGCGAAAGTTTTGTTCTTCTGAAGAATGCCCCCGCCACCGCACAGCCACTCGCAGCTCATAGCTCGTCACCCGTAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAAGGTACAGTTGCCGTCATCGGCCCTCTCGGAAATACCCGCAGCAACATGCCGGGCACCTGGAGCGTAGCCGCACGCCTCAACGATTATCCTTCTTTATACGAAGGCTTGAAAGAAATGATGGCAGGCAAGGTGAACATCACCTATGCCAAAGGTAGTAACCTCATCGGCGATGCAGCTTACGAAGAACGTGCCACCATGTTCGGCCGTTCATTGAACCGCGATAATCGCACGGACCAGGAGTTACTGGACGAAGCACTGAAAATTGCAGCCGGCGCCGATGTTATCGTAGCTGCCCTGGGAGAATCTTCTGAAATGAGCGGTGAAAGTTCAAGCCGCACCGAACTCGGCTTGCCCGATGTACAACATACCCTGTTGGAAGCCTTACTGAAAACGGGTAAACCCGTAGTACTAACCCTCTTTACCGGTCGGCCGTTGACGCTGAACTGGGAACAGGAGCATGTACCTGCCATCTTGAATGTATGGTTCGGAGGCAGTGAAGCGGCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTCAATCCGAGTGGAAAACTAACCATGACTTTCCCGAAGAATGTAGGCCAGATACCTTTGTTCTACAATCATAAGAATACCGGTCGGCCACTGGCGGCAGGCAAATGGTTCGAAAAGTTCCGTTCAAACTATCTGGATGTGGATAACGAACCGCTGTATCCCTTCGGTTATGGATTGTCGTATACCACTTTCCAGTACAGTGACATTGCATTGAGCACACCGACATTGGGAAAAGATGGTTCCGTTACAGCCGTAGTCACCGTCACCAATACTGGTAAACATGACGGTGCGGAAGTAGTTCAACTCTATATCCGCGACCTCGTAGGAAGTATCACCCGCCCTGTACGCGAGTTGAAAGGTTTCAATAAAATCTTCCTTCGCGCCGGAGAAAGCAAAACGGTATCATTCACTATCACGCGTGATCTGCTTCGCTTCTATGATTACGACCTGAACTACGTAGCCGAACCGGGTGACTTTGACATCATGATCGGTGGAAACAGCCAGGCTGTGAAGACGGCGAAGTTGACACTT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,700 total letters Query= APC115038.107 Length=2679 Score E Sequences producing significant alignments: (Bits) Value wt 4087 0.0 07B_B1_APC115038.107_B01.seq 1121 0.0 01B_B1_APC115038.107_B01.seq 747 0.0 K S P Q D M D R F I D A L M K K M T V E E K I G Q L N L P V Query_1 202 AAGTCACCGCAAGACATGGATCGCTTCATCGACGCACTGATGAAGAAGATGACCGTGGAAGAGAAAATCGGACAATTGAACCTACCCGTC 291 2 1 .......................................................................................... 90 T G D I T T G Q A K S S D V A Q K I E K G L V G G L F N L K Query_1 292 ACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCACAAAAGATTGAAAAAGGATTGGTGGGCGGACTCTTCAACCTAAAA 381 2 91 .......................................................................................... 180 G V D R I L E V Q K L A V E K S R L G I P L L F G M D V I H Query_1 382 GGTGTAGACCGTATTCTTGAAGTGCAAAAGCTGGCAGTAGAGAAATCACGCCTCGGTATTCCCCTGCTGTTCGGCATGGATGTGATACAT 471 2 181 .......................................................................................... 270 G Y E T I F P I P L G L S C T W D M A A I E K S A R I A A I Query_1 472 GGCTACGAAACCATCTTCCCCATTCCATTGGGATTGTCCTGCACCTGGGATATGGCGGCTATCGAGAAATCCGCCCGTATTGCAGCCATC 561 2 271 .......................................................................................... 360 E A S A D G I S W T F S P M V D I S R D P R W G R V S E G S Query_1 562 GAAGCAAGTGCCGATGGCATTTCCTGGACATTCAGTCCGATGGTAGACATCAGTCGCGACCCACGTTGGGGACGTGTCAGCGAGGGCTCG 651 2 361 .......................................................................................... 450 G E D P F L G G A I A Q A M V Y G Y Q G A N L Q D Q L H R N Query_1 652 GGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTATACGGATACCAGGGTGCCAATCTGCAAGACCAATTGCACCGCAAC 741 2 451 .......................................................................................... 540 D E I M A C V K H F A L Y G A G E A G R D Y N T V D M S R N Query_1 742 GATGAAATCATGGCTTGCGTAAAACACTTTGCATTGTATGGAGCCGGAGAAGCCGGACGCGACTATAATACAGTAGATATGAGCCGCAAC 831 2 541 .......................................................................................... 630 R M F N E F M Y P Y E A A V E A G V G S V M A S F N E I D G Query_1 832 CGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAGGCCGGAGTGGGTAGTGTGATGGCGTCATTCAATGAAATAGACGGT 921 2 631 .......................................................................................... 720 I P A T G N K W L L S D L L R G Q W G F E G F V V T D F T Query_1 922 ATTCCCGCCACCGGAAACAAATGGCTGCTGAGCGATTTGCTGCGTGGCCAGTGGGGCTTCGAAGGGTTTG-TGGTAACG-GACTTC-ACA 1008 2 721 ......................................................................-........-......-... 807 1 871 .....AN...G...N..N.N.GN...N... 842 G I S E M I E H G V G D L Q T V S A L A L N A G V Query_1 1009 GGC--ATTTCAGAGATGAT-AGAGCA-TGGTGT-CGGCG-ACT-TGC-AAACCG--TCAGTGCACTCGC--TCTTAATG-CAGGGGTGGA 1085 2 808 ...--..............-......-......-.....-...-...-......--.............--........-.......... 884 1 841 ...CAN......N....N.T....N.N......TN..N.N.N.N.A.N.....NNN............ANT..NN...NN......N... 752 D M D M V S E G F V G T L M K S I K E G K V R M G T L Query_1 1086 -CA-TGGATATGGT-AAGTGA--GGGCTTCGTC-GGTACAC-TGATGAAAT-CAATTAAAGAAGGAAAAGTAAGAATGGG-CACG-TTGA 1165 2 885 -..-..........-......--..........-.......-.........-............................-....-.... 964 1 751 A..N..N......NN...N..AG...T..N...C...N.G.C.........N......N...............N.....G....G.... 662 N T A C R R I L E A K Y K L G L F D N P Y K Y C D V N R Query_1 1166 ATAC-AGCCTGC-CGCCGGATATTGG-AAGCGAAA-TACAAGC-TGGGACT-GTTTGACAATCCTTATAAATACTGCGACGTGAACCGTC 1249 2 965 ....-.......-.............-........-.......-.......-...................................... 1048 1 661 ....C.....N.N...N.........GN.......A......NN.......T........................N............. 572 P K R D I F T K E H R D A A R K I A G E S F V L L K N A P Query_1 1250 CGAAGC--GGGATATCTTCA-CAAAAGAGCATCGTGACGCCGCCCGCAAGATTGCCGGCGAAAGTTTTGTTCTTCTGAAGAATGCCCCCG 1336 2 1049 ......--............-..................................................................... 1135 1 571 N....GCG............A................N......N.............N............................... 482 A T A Q P L A A H S S S P V T A S P V L P L K K Q G T V A V Query_1 1337 CCACCGCACAGCCACTCGCAGCTCATAGCTCGTCACCCGTAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAAGGTACAGTTGCCGTCA 1426 2 1136 .......................................................................................... 1225 1 481 ................N......................................................................... 392 0 62 ........... 72 I G P L G N T R S N M P G T A S V A A R L N D Y P S L Y E G Query_1 1427 TCGGCCCTCTCGGAAATACCCGCAGCAACATGCCGGGCACCGCTAGCGTAGCCGCACGCCTCAACGATTATCCTTCTTTATACGAAGGCT 1516 2 1226 .........................................TGG.............................................. 1315 1 391 .......................................................................................... 302 L K E M M A G K V N I T Y A K G S N L I G D A A Y E E R A T Query_1 1517 TGAAAGAAATGATGGCAGGCAAGGTGAACATCACCTATGCCAAAGGTAGTAACCTCATCGGCGATGCAGCTTACGAAGAACGTGCCACCA 1606 2 1316 .......................................................................................... 1405 1 301 .......................................................................................... 212 0 173 ..N..T......N..NT..N..N.GG.....T......G.NN.....N..NN.NN...GC...NN...NTTG...N..... 253 M F G R S L N R D N R T D Q E L L D E A L K I A A G A D V I Query_1 1607 TGTTCGGCCGTTCATTGAACCGCGATAATCGCACGGACCAGGAGTTACTGGACGAAGCACTGAAAATTGCAGCCGGCGCCGATGTTATCG 1696 2 1406 .......................................................................................... 1495 1 211 .......................................................................................... 122 0 254 ..NN...NG.NN.TG..N..NN.NN.NN...N....TG..NCNNNN............N......C...N.N..N.A...N...G.C..N 343 V A A L G E S S E M S G E S S S R T E L G L P D V Q H T L Query_1 1697 TA-GCTGCCCTGGGAGAATCTTCTGAAATGAGCGGTGAAAGTTCAAGCCGCACCGAACTCGGCTTGCC-CGATGTACAACATA-CCCTGT 1783 2 1496 ..-.................................................................-..............-...... 1582 1 121 ..T.................................................................A..............T...... 32 0 344 NC-C....N.ATN....NC.A........TTCTTC.....ANGN.-......................-..............-...... 429 L E A L L K T G K P V V L T L F T G R P L T L N W E Q E H V Query_1 1784 TGGAAGCCTTACTGAAAACGGGTAAACCCGTAGTACTAACCCTCTTTACCGGTCGGCCGTTGACGCTGAACTGGGAACAGGAGCATGTAC 1873 2 1583 .......................................................................................... 1672 2 1821 ............ 1832 1 31 ..................NC........ 4 0 430 ............N.............N....................T.........................N................ 519 P A I L N V W F G G S E A A Y A I G D V L F G D V N P S G K Query_1 1874 CTGCCATCTTGAATGTATGGTTCGGAGGCAGTGAAGCGGCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTCAATCCGAGTGGAAAAC 1963 2 1673 .......................................................................................... 1762 0 520 .......................N..N..N.....NN...................................N.....NN.......... 609 L T M T F P K N V G Q I P L F Y N H K N T G R P L A A G K W Query_1 1964 TAACCATGACTTTCCCGAAGAATGTAGGCCAGATACCTTTGTTCTACAATCATAAGAATACCGGTCGGCCACTGGCGGCAGGCAAATGGT 2053 2 1763 .......................................................................................... 1852 2 1629 ............ 1640 0 610 ...................N......N................NCTT........NNT..N..N....N......NN...N......... 699 F E K F R S N Y L D V D N E P L Y P F G Y G L S Y T T F Q Y Query_1 2054 TCGAAAAGTTCCGTTCAAACTATCTGGATGTGGATAACGAACCGCTGTATCCC-TTCGGTTATGGATTGTCGTATACCACTTTCCAGTAC 2142 2 1853 .....................................................-.................................... 1941 0 700 ..N..........N........N...............N.....T........T.......N.......N.NN............NA... 789 S D I A L S T P T L G K D G S V T A V V T V T N T G K H D G Query_1 2143 AGTGACATTGCATTGAGCACACCGACATTGGGAAAAGATGGTTCCGTTACAGCCGTAGTCACCGTCACCAATACTGGTAAACATGACGGT 2232 2 1942 .......................................................................................... 2031 0 790 -N....N..........-TN.....NNN....G.........N..NN...TN.N.C......N..-NN..N......N........NT.. 876 A E V V Q L Y I R D L V G S I T R P V R E L K G F N K I F Query_1 2233 GCGGAAGTAGTTCAACTCTATATCCGCGACCTCGTAGGAAGTATCACCCG-CCCTGTACGCGAGTTGAAAGGTTTCAATA-AAATCTTCC 2320 2 2032 ..................................................-.............................-......... 2119 0 877 ....-NN.N....W..........NNTN.N.N.N.TA....CN.......A......NNC.C.....N.NA.G.......T......... 965 L R A G E S K T V S F T I T R D L L R F Y D Y D L N Y V A E Query_1 2321 TTCGCGCCGGAGAAAGCAAAACGGTATCATTCACTATCACGCGTGATCTGCTTCGCTTCTATGATTACGACCTGAACTACGTAGCCGAAC 2410 2 2120 .......................................................................................... 2209 0 966 ...NNN.N...T.T.--N.....C....T.N..N.N...A.. 1005 P G D F D I M I G G N S Q A V K T A K L T L Query_1 2411 CGGGTGACTTTGACATCATGATCGGTGGAAACAGCCAGGCTGTGAAGACGGCGAAGTTGACACTT 2475 2 2210 ................................................................. 2274 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12375638 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:06 PM Number of letters in database: 4,700 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115038.111 (APC115038.26-783.P(785).K213A.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVTGDITTGQAKSSDVAQKIEKGLVGGLFNLKGVDRILEVQKLAVEKSRLGIPLLFGMDVIHGYETIFPIPLGLSCTWDMAAIEKSARIAAIEASADGISWTFSPMVDISRDPRWGRVSEGSGEDPFLGGAIAQAMVYGYQGANLQDQLHRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEFMYPYEAAVEAGVGSVMASFNEIDGIPATGNKWLLSDLLRGQWGFEGFVVTDFTGISEMIEHGVGDLQTVSALALNAGVDMDMVSEGFVGTLMKSIKEGKVRMGTLNTACRRILEAKYKLGLFDNPYKYCDVNRPKRDIFTKEHRDAARKIAGESFVLLKNAPATAQPLAAHSSSPVTASPVLPLKKQGTVAVIGPLGNTRSNMPGTWSVAARLNDYPSLYEGLKEMMAGKVNITYAKGSNLIGDAAYEERATMFGRSLNRDNRTDQELLDEALKIAAGADVIVAALGESSEMSGESSSRTELGLPDVQHTLLEALLKTGKPVVLTLFTGRPLTLNWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLAAGKWFEKFRSNYLDVDNEPLYPFGYGLSYTTFQYSDIALSTPTLGKDGSVTAVVTVTNTGKHDGAEVVQLYIRDLVGSITRPVRELKGFNKIFLRAGESKTVSFTITRDLLRFYDYDLNYVAEPGDFDIMIGGNSQAVKTAKLTL
AAGTCACCGCAAGACATGGATCGCTTCATCGACGCACTGATGAAGAAGATGACCGTGGAAGAGAAAATCGGACAATTGAACCTACCCGTCACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCACAAAAGATTGAAAAAGGATTGGTGGGCGGACTCTTCAACCTAAAAGGTGTAGACCGTATTCTTGAAGTGCAAAAGCTGGCAGTAGAGAAATCACGCCTCGGTATTCCCCTGCTGTTCGGCATGGATGTGATACATGGCTACGAAACCATCTTCCCCATTCCATTGGGATTGTCCTGCACCTGGGATATGGCGGCTATCGAGAAATCCGCCCGTATTGCAGCCATCGAAGCAAGTGCCGATGGCATTTCCTGGACATTCAGTCCGATGGTAGACATCAGTCGCGACCCACGTTGGGGACGTGTCAGCGAGGGCTCGGGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTATACGGATACCAGGGTGCCAATCTGCAAGACCAATTGCACCGCAACGATGAAATCATGGCTTGCGTAAAACACTTTGCATTGTATGGAGCCGGAGAAGCCGGACGCGACTATAATACAGTAGATATGAGCCGCAACCGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAGGCCGGAGTGGGTAGTGTGATGGCGTCATTCAATGAAATAGACGGTATTCCCGCCACCGGAAACAAATGGCTGCTGAGCGATTTGCTGCGTGGCCAGTGGGGCTTCGAAGGGTTTGTGGTAACGGACTTCACAGGCATTTCAGAGATGATAGAGCATGGTGTCGGCGACTTGCAAACCGTCAGTGCACTCGCTCTTAATGCAGGGGTGGACATGGATATGGTAAGTGAGGGCTTCGTCGGTACACTGATGAAATCAATTAAAGAAGGAAAAGTAAGAATGGGCACGTTGAATACAGCCTGCCGCCGGATATTGGAAGCGAAATACAAGCTGGGACTGTTTGACAATCCTTATAAATACTGCGACGTGAACCGTCCGAAGCGGGATATCTTCACAAAAGAGCATCGTGACGCCGCCCGCAAGATTGCCGGCGAAAGTTTTGTTCTTCTGAAGAATGCCCCCGCCACCGCACAGCCACTCGCAGCTCATAGCTCGTCACCCGTAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAAGGTACAGTTGCCGTCATCGGCCCTCTCGGAAATACCCGCAGCAACATGCCGGGCACCTGGAGCGTAGCCGCACGCCTCAACGATTATCCTTCTTTATACGAAGGCTTGAAAGAAATGATGGCAGGCAAGGTGAACATCACCTATGCCAAAGGTAGTAACCTCATCGGCGATGCAGCTTACGAAGAACGTGCCACCATGTTCGGCCGTTCATTGAACCGCGATAATCGCACGGACCAGGAGTTACTGGACGAAGCACTGAAAATTGCAGCCGGCGCCGATGTTATCGTAGCTGCCCTGGGAGAATCTTCTGAAATGAGCGGTGAAAGTTCAAGCCGCACCGAACTCGGCTTGCCCGATGTACAACATACCCTGTTGGAAGCCTTACTGAAAACGGGTAAACCCGTAGTACTAACCCTCTTTACCGGTCGGCCGTTGACGCTGAACTGGGAACAGGAGCATGTACCTGCCATCTTGAATGTATGGTTCGGAGGCAGTGAAGCGGCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTCAATCCGAGTGGAAAACTAACCATGACTTTCCCGAAGAATGTAGGCCAGATACCTTTGTTCTACAATCATAAGAATACCGGTCGGCCACTGGCGGCAGGCAAATGGTTCGAAAAGTTCCGTTCAAACTATCTGGATGTGGATAACGAACCGCTGTATCCCTTCGGTTATGGATTGTCGTATACCACTTTCCAGTACAGTGACATTGCATTGAGCACACCGACATTGGGAAAAGATGGTTCCGTTACAGCCGTAGTCACCGTCACCAATACTGGTAAACATGACGGTGCGGAAGTAGTTCAACTCTATATCCGCGACCTCGTAGGAAGTATCACCCGCCCTGTACGCGAGTTGAAAGGTTTCAATAAAATCTTCCTTCGCGCCGGAGAAAGCAAAACGGTATCATTCACTATCACGCGTGATCTGCTTCGCTTCTATGATTACGACCTGAACTACGTAGCCGAACCGGGTGACTTTGACATCATGATCGGTGGAAACAGCCAGGCTGTGAAGACGGCGAAGTTGACACTT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,743 total letters Query= APC115038.111 Length=2679 Score E Sequences producing significant alignments: (Bits) Value wt 4087 0.0 01C_C1_APC115038.111_C01.seq 1905 0.0 07C_C1_APC115038.111_C01.seq 1867 0.0 S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * E Query_1 5 GATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAG 94 1 1140 ..N..N.....--..............NNN...-...NTNAN....GAN...N.N..--.......T--.N..............-.N.. 1059 G D I H M H H H H H H S S G V D L W S H P Q F E K G T E N L Query_1 95 GAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACC-TG 183 1 1058 .....N-........N...................................................................G...C.. 970 Y F Q S N A K S P Q D M D R F I D A L M K K M T V E E K I G Query_1 184 TACTTCCAATCCAATGCCAAGTCACCGCAAGACATGGATCGCTTCATCGACGCACTGATGAAGAAGATGACCGTGGAAGAGAAAATCGGA 273 2 1 ........................................................................ 72 1 969 ................T......................................................................... 880 Q L N L P V T G D I T T G Q A K S S D V A Q K I E K G L V G Query_1 274 CAATTGAACCTACCCGTCACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCACAAAAGATTGAAAAAGGATTGGTGGGC 363 2 73 .......................................................................................... 162 1 879 .......................................................................................... 790 G L F N L K G V D R I L E V Q K L A V E K S R L G I P L L F Query_1 364 GGACTCTTCAACCTAAAAGGTGTAGACCGTATTCTTGAAGTGCAAAAGCTGGCAGTAGAGAAATCACGCCTCGGTATTCCCCTGCTGTTC 453 2 163 .......................................................................................... 252 1 789 .......................................................................................... 700 G M D V I H G Y E T I F P I P L G L S C T W D M A A I E K S Query_1 454 GGCATGGATGTGATACATGGCTACGAAACCATCTTCCCCATTCCATTGGGATTGTCCTGCACCTGGGATATGGCGGCTATCGAGAAATCC 543 2 253 .......................................................................................... 342 1 699 .......................................................................................... 610 A R I A A I E A S A D G I S W T F S P M V D I S R D P R W G Query_1 544 GCCCGTATTGCAGCCATCGAAGCAAGTGCCGATGGCATTTCCTGGACATTCAGTCCGATGGTAGACATCAGTCGCGACCCACGTTGGGGA 633 2 343 .......................................................................................... 432 1 609 .......................................................................................... 520 R V S E G S G E D P F L G G A I A Q A M V Y G Y Q G A N L Q Query_1 634 CGTGTCAGCGAGGGCTCGGGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTATACGGATACCAGGGTGCCAATCTGCAA 723 2 433 .......................................................................................... 522 0 22 .....-................................ 58 1 519 .......................................................................................... 430 D Q L H R N D E I M A C V A H F A L Y G A G E A G R D Y N T Query_1 724 GACCAATTGCACCGCAACGATGAAATCATGGCTTGCGTAGCTCACTTTGCATTGTATGGAGCCGGAGAAGCCGGACGCGACTATAATACA 813 2 523 .......................................AAA................................................ 612 0 59 ....................A..................................................................... 148 1 429 .......................................................................................... 340 V D M S R N R M F N E F M Y P Y E A A V E A G V G S V M A S Query_1 814 GTAGATATGAGCCGCAACCGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAGGCCGGAGTGGGTAGTGTGATGGCGTCA 903 2 613 .......................................................................................... 702 0 149 .......................................................................................... 238 1 339 .......................................................................................... 250 F N E I D G I P A T G N K W L L S D L L R G Q W G F E G F V Query_1 904 TTCAATGAAATAGACGGTATTCCCGCCACCGGAAACAAATGGCTGCTGAGCGATTTGCTGCGTGGCCAGTGGGGCTTCGAAGGGTTTGTG 993 2 703 .......................................................................................... 792 0 239 .......................................................................................... 328 1 249 .......................................................................................... 160 V T D F T G I S E M I E H G V G D L Q T V S A L A L N A G V Query_1 994 GTAACGGACTTCACAGGCATTTCAGAGATGATAGAGCATGGTGTCGGCGACTTGCAAACCGTCAGTGCACTCGCTCTTAATGCAGGGGTG 1083 2 793 .......................................................................................... 882 0 329 .......................................................................................... 418 1 159 .......................................................................................... 70 D M D M V S E G F V G T L M K S I K E G K V R M G T L N T A Query_1 1084 GACATGGATATGGTAAGTGAGGGCTTCGTCGGTACACTGATGAAATCAATTAAAGAAGGAAAAGTAAGAATGGGCACGTTGAATACAGCC 1173 2 883 .......................................................................................... 972 0 419 .......................................................................................... 508 1 69 ........................................ 30 C R R I L E A K Y K L G L F D N P Y K Y C D V N R P K R D I Query_1 1174 TGCCGCCGGATATTGGAAGCGAAATACAAGCTGGGACTGTTTGACAATCCTTATAAATACTGCGACGTGAACCGTCCGAAGCGGGATATC 1263 2 973 .......................................................................................... 1062 0 509 .......................................................................................... 598 F T K E H R D A A R K I A G E S F V L L K N A P A T A Q P L Query_1 1264 TTCACAAAAGAGCATCGTGACGCCGCCCGCAAGATTGCCGGCGAAAGTTTTGTTCTTCTGAAGAATGCCCCCGCCACCGCACAGCCACTC 1353 2 1063 .......................................................................................... 1152 0 599 .......................................................................................... 688 A A H S S S P V T A S P V L P L K K Q G T V A V I G P L G N Query_1 1354 GCAGCTCATAGCTCGTCACCCGTAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAAGGTACAGTTGCCGTCATCGGCCCTCTCGGAAAT 1443 2 1153 .......................................................................................... 1242 0 689 .......................................................................................... 778 T R S N M P G T W S V A A R L N D Y P S L Y E G L K E M M A Query_1 1444 ACCCGCAGCAACATGCCGGGCACCTGGAGCGTAGCCGCACGCCTCAACGATTATCCTTCTTTATACGAAGGCTTGAAAGAAATGATGGCA 1533 2 1243 .......................................................................................... 1332 0 779 ...........................................................................-.............. 867 G K V N I T Y A K G S N L I G D A A Y E E R A T M F G R S L Query_1 1534 GGCAAGGTGAACATCACCTATGCCAAAGGTAGTAACCTCATCGGCGATGCAGCTTACGAAGAACGTGCCACCATGTTCGGCCGTTCATTG 1623 2 1333 .......................................................................................... 1422 0 868 .......................................................................................... 957 N R D N R T D Q E L L D E A L K I A A G A D V I V A A L G Query_1 1624 AACCGCGATAATCGCACGGACCAGGAGTTACTGGACGAAGC-ACTGAAAATTGCAGCCGGCGCCGATGTTATCGTAGCTGCCCTGGGAGA 1712 2 1423 .........................................-................................................ 1511 0 958 .........................................N.N.....T.GC.......N.......-..................-.. 1045 E S S E M S G E S S S R T E L G L P D V Q H T L L E A L L K Query_1 1713 ATCTTCTGAAATGAGCGGTGAAAGTTCAAGCCGCACCGAACTCGGCTTGCCCGATGTACAACATACCCTGTTGGAAGCCTTACTGAAAAC 1802 2 1512 .......................................................................................... 1601 0 1046 GANC....-.....N.N..............NCN....-............................N....................-- 1131 T G K P V V L T L F T G R P L T L N W E Q E H V P A I L N V Query_1 1803 GGGTAAACCCGTAGTACTAACCCTCTTTACCGGTCGGCCGTTGACGCTGAACTGGGAACAGGAGCATGTACCTGCCATCTTGAATGTATG 1892 2 1602 .......................................................................................... 1691 2 1821 ............ 1832 0 1132 C...N.CN...N.NN....C..N..-.......... 1166 W F G G S E A A Y A I G D V L F G D V N P S G K L T M T F P Query_1 1893 GTTCGGAGGCAGTGAAGCGGCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTCAATCCGAGTGGAAAACTAACCATGACTTTCCCGAA 1982 2 1692 .......................................................................................... 1781 K N V G Q I P L F Y N H K N T G R P L A A G K W F E K F R S Query_1 1983 GAATGTAGGCCAGATACCTTTGTTCTACAATCATAAGAATACCGGTCGGCCACTGGCGGCAGGCAAATGGTTCGAAAAGTTCCGTTCAAA 2072 2 1782 .......................................................................................... 1871 2 1629 ............ 1640 N Y L D V D N E P L Y P F G Y G L S Y T T F Q Y S D I A L S Query_1 2073 CTATCTGGATGTGGATAACGAACCGCTGTATCCCTTCGGTTATGGATTGTCGTATACCACTTTCCAGTACAGTGACATTGCATTGAGCAC 2162 2 1872 .......................................................................................... 1961 T P T L G K D G S V T A V V T V T N T G K H D G A E V V Q L Query_1 2163 ACCGACATTGGGAAAAGATGGTTCCGTTACAGCCGTAGTCACCGTCACCAATACTGGTAAACATGACGGTGCGGAAGTAGTTCAACTCTA 2252 2 1962 .......................................................................................... 2051 Y I R D L V G S I T R P V R E L K G F N K I F L R A G E S K Query_1 2253 TATCCGCGACCTCGTAGGAAGTATCACCCGCCCTGTACGCGAGTTGAAAGGTTTCAATAAAATCTTCCTTCGCGCCGGAGAAAGCAAAAC 2342 2 2052 .......................................................................................... 2141 T V S F T I T R D L L R F Y D Y D L N Y V A E P G D F D I M Query_1 2343 GGTATCATTCACTATCACGCGTGATCTGCTTCGCTTCTATGATTACGACCTGAACTACGTAGCCGAACCGGGTGACTTTGACATCATGAT 2432 2 2142 .......................................................................................... 2231 I G G N S Q A V K T A K L T L Query_1 2433 CGGTGGAAACAGCCAGGCTGTGAAGACGGCGAAGTTGACACTT 2475 2 2232 ........................................... 2274 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12490104 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:06 PM Number of letters in database: 4,743 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115038.112 (APC115038.26-783.P(785).H214A.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVTGDITTGQAKSSDVAQKIEKGLVGGLFNLKGVDRILEVQKLAVEKSRLGIPLLFGMDVIHGYETIFPIPLGLSCTWDMAAIEKSARIAAIEASADGISWTFSPMVDISRDPRWGRVSEGSGEDPFLGGAIAQAMVYGYQGANLQDQLHRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEFMYPYEAAVEAGVGSVMASFNEIDGIPATGNKWLLSDLLRGQWGFEGFVVTDFTGISEMIEHGVGDLQTVSALALNAGVDMDMVSEGFVGTLMKSIKEGKVRMGTLNTACRRILEAKYKLGLFDNPYKYCDVNRPKRDIFTKEHRDAARKIAGESFVLLKNAPATAQPLAAHSSSPVTASPVLPLKKQGTVAVIGPLGNTRSNMPGTWSVAARLNDYPSLYEGLKEMMAGKVNITYAKGSNLIGDAAYEERATMFGRSLNRDNRTDQELLDEALKIAAGADVIVAALGESSEMSGESSSRTELGLPDVQHTLLEALLKTGKPVVLTLFTGRPLTLNWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLAAGKWFEKFRSNYLDVDNEPLYPFGYGLSYTTFQYSDIALSTPTLGKDGSVTAVVTVTNTGKHDGAEVVQLYIRDLVGSITRPVRELKGFNKIFLRAGESKTVSFTITRDLLRFYDYDLNYVAEPGDFDIMIGGNSQAVKTAKLTL
AAGTCACCGCAAGACATGGATCGCTTCATCGACGCACTGATGAAGAAGATGACCGTGGAAGAGAAAATCGGACAATTGAACCTACCCGTCACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCACAAAAGATTGAAAAAGGATTGGTGGGCGGACTCTTCAACCTAAAAGGTGTAGACCGTATTCTTGAAGTGCAAAAGCTGGCAGTAGAGAAATCACGCCTCGGTATTCCCCTGCTGTTCGGCATGGATGTGATACATGGCTACGAAACCATCTTCCCCATTCCATTGGGATTGTCCTGCACCTGGGATATGGCGGCTATCGAGAAATCCGCCCGTATTGCAGCCATCGAAGCAAGTGCCGATGGCATTTCCTGGACATTCAGTCCGATGGTAGACATCAGTCGCGACCCACGTTGGGGACGTGTCAGCGAGGGCTCGGGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTATACGGATACCAGGGTGCCAATCTGCAAGACCAATTGCACCGCAACGATGAAATCATGGCTTGCGTAAAACACTTTGCATTGTATGGAGCCGGAGAAGCCGGACGCGACTATAATACAGTAGATATGAGCCGCAACCGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAGGCCGGAGTGGGTAGTGTGATGGCGTCATTCAATGAAATAGACGGTATTCCCGCCACCGGAAACAAATGGCTGCTGAGCGATTTGCTGCGTGGCCAGTGGGGCTTCGAAGGGTTTGTGGTAACGGACTTCACAGGCATTTCAGAGATGATAGAGCATGGTGTCGGCGACTTGCAAACCGTCAGTGCACTCGCTCTTAATGCAGGGGTGGACATGGATATGGTAAGTGAGGGCTTCGTCGGTACACTGATGAAATCAATTAAAGAAGGAAAAGTAAGAATGGGCACGTTGAATACAGCCTGCCGCCGGATATTGGAAGCGAAATACAAGCTGGGACTGTTTGACAATCCTTATAAATACTGCGACGTGAACCGTCCGAAGCGGGATATCTTCACAAAAGAGCATCGTGACGCCGCCCGCAAGATTGCCGGCGAAAGTTTTGTTCTTCTGAAGAATGCCCCCGCCACCGCACAGCCACTCGCAGCTCATAGCTCGTCACCCGTAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAAGGTACAGTTGCCGTCATCGGCCCTCTCGGAAATACCCGCAGCAACATGCCGGGCACCTGGAGCGTAGCCGCACGCCTCAACGATTATCCTTCTTTATACGAAGGCTTGAAAGAAATGATGGCAGGCAAGGTGAACATCACCTATGCCAAAGGTAGTAACCTCATCGGCGATGCAGCTTACGAAGAACGTGCCACCATGTTCGGCCGTTCATTGAACCGCGATAATCGCACGGACCAGGAGTTACTGGACGAAGCACTGAAAATTGCAGCCGGCGCCGATGTTATCGTAGCTGCCCTGGGAGAATCTTCTGAAATGAGCGGTGAAAGTTCAAGCCGCACCGAACTCGGCTTGCCCGATGTACAACATACCCTGTTGGAAGCCTTACTGAAAACGGGTAAACCCGTAGTACTAACCCTCTTTACCGGTCGGCCGTTGACGCTGAACTGGGAACAGGAGCATGTACCTGCCATCTTGAATGTATGGTTCGGAGGCAGTGAAGCGGCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTCAATCCGAGTGGAAAACTAACCATGACTTTCCCGAAGAATGTAGGCCAGATACCTTTGTTCTACAATCATAAGAATACCGGTCGGCCACTGGCGGCAGGCAAATGGTTCGAAAAGTTCCGTTCAAACTATCTGGATGTGGATAACGAACCGCTGTATCCCTTCGGTTATGGATTGTCGTATACCACTTTCCAGTACAGTGACATTGCATTGAGCACACCGACATTGGGAAAAGATGGTTCCGTTACAGCCGTAGTCACCGTCACCAATACTGGTAAACATGACGGTGCGGAAGTAGTTCAACTCTATATCCGCGACCTCGTAGGAAGTATCACCCGCCCTGTACGCGAGTTGAAAGGTTTCAATAAAATCTTCCTTCGCGCCGGAGAAAGCAAAACGGTATCATTCACTATCACGCGTGATCTGCTTCGCTTCTATGATTACGACCTGAACTACGTAGCCGAACCGGGTGACTTTGACATCATGATCGGTGGAAACAGCCAGGCTGTGAAGACGGCGAAGTTGACACTT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,284 total letters Query= APC115038.112 Length=2679 Score E Sequences producing significant alignments: (Bits) Value wt 4087 0.0 K S P Q D M D R F I D A L M K K M T V E E K I G Q L N L P V Query_1 202 AAGTCACCGCAAGACATGGATCGCTTCATCGACGCACTGATGAAGAAGATGACCGTGGAAGAGAAAATCGGACAATTGAACCTACCCGTC 291 2 1 .......................................................................................... 90 T G D I T T G Q A K S S D V A Q K I E K G L V G G L F N L K Query_1 292 ACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCACAAAAGATTGAAAAAGGATTGGTGGGCGGACTCTTCAACCTAAAA 381 2 91 .......................................................................................... 180 G V D R I L E V Q K L A V E K S R L G I P L L F G M D V I H Query_1 382 GGTGTAGACCGTATTCTTGAAGTGCAAAAGCTGGCAGTAGAGAAATCACGCCTCGGTATTCCCCTGCTGTTCGGCATGGATGTGATACAT 471 2 181 .......................................................................................... 270 G Y E T I F P I P L G L S C T W D M A A I E K S A R I A A I Query_1 472 GGCTACGAAACCATCTTCCCCATTCCATTGGGATTGTCCTGCACCTGGGATATGGCGGCTATCGAGAAATCCGCCCGTATTGCAGCCATC 561 2 271 .......................................................................................... 360 E A S A D G I S W T F S P M V D I S R D P R W G R V S E G S Query_1 562 GAAGCAAGTGCCGATGGCATTTCCTGGACATTCAGTCCGATGGTAGACATCAGTCGCGACCCACGTTGGGGACGTGTCAGCGAGGGCTCG 651 2 361 .......................................................................................... 450 G E D P F L G G A I A Q A M V Y G Y Q G A N L Q D Q L H R N Query_1 652 GGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTATACGGATACCAGGGTGCCAATCTGCAAGACCAATTGCACCGCAAC 741 2 451 .......................................................................................... 540 D E I M A C V K A F A L Y G A G E A G R D Y N T V D M S R N Query_1 742 GATGAAATCATGGCTTGCGTAAAAGCTTTTGCATTGTATGGAGCCGGAGAAGCCGGACGCGACTATAATACAGTAGATATGAGCCGCAAC 831 2 541 ........................CAC............................................................... 630 R M F N E F M Y P Y E A A V E A G V G S V M A S F N E I D G Query_1 832 CGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAGGCCGGAGTGGGTAGTGTGATGGCGTCATTCAATGAAATAGACGGT 921 2 631 .......................................................................................... 720 I P A T G N K W L L S D L L R G Q W G F E G F V V T D F T G Query_1 922 ATTCCCGCCACCGGAAACAAATGGCTGCTGAGCGATTTGCTGCGTGGCCAGTGGGGCTTCGAAGGGTTTGTGGTAACGGACTTCACAGGC 1011 2 721 .......................................................................................... 810 I S E M I E H G V G D L Q T V S A L A L N A G V D M D M V S Query_1 1012 ATTTCAGAGATGATAGAGCATGGTGTCGGCGACTTGCAAACCGTCAGTGCACTCGCTCTTAATGCAGGGGTGGACATGGATATGGTAAGT 1101 2 811 .......................................................................................... 900 E G F V G T L M K S I K E G K V R M G T L N T A C R R I L E Query_1 1102 GAGGGCTTCGTCGGTACACTGATGAAATCAATTAAAGAAGGAAAAGTAAGAATGGGCACGTTGAATACAGCCTGCCGCCGGATATTGGAA 1191 2 901 .......................................................................................... 990 A K Y K L G L F D N P Y K Y C D V N R P K R D I F T K E H R Query_1 1192 GCGAAATACAAGCTGGGACTGTTTGACAATCCTTATAAATACTGCGACGTGAACCGTCCGAAGCGGGATATCTTCACAAAAGAGCATCGT 1281 2 991 .......................................................................................... 1080 D A A R K I A G E S F V L L K N A P A T A Q P L A A H S S S Query_1 1282 GACGCCGCCCGCAAGATTGCCGGCGAAAGTTTTGTTCTTCTGAAGAATGCCCCCGCCACCGCACAGCCACTCGCAGCTCATAGCTCGTCA 1371 2 1081 .......................................................................................... 1170 P V T A S P V L P L K K Q G T V A V I G P L G N T R S N M P Query_1 1372 CCCGTAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAAGGTACAGTTGCCGTCATCGGCCCTCTCGGAAATACCCGCAGCAACATGCCG 1461 2 1171 .......................................................................................... 1260 G T W S V A A R L N D Y P S L Y E G L K E M M A G K V N I T Query_1 1462 GGCACCTGGAGCGTAGCCGCACGCCTCAACGATTATCCTTCTTTATACGAAGGCTTGAAAGAAATGATGGCAGGCAAGGTGAACATCACC 1551 2 1261 .......................................................................................... 1350 Y A K G S N L I G D A A Y E E R A T M F G R S L N R D N R T Query_1 1552 TATGCCAAAGGTAGTAACCTCATCGGCGATGCAGCTTACGAAGAACGTGCCACCATGTTCGGCCGTTCATTGAACCGCGATAATCGCACG 1641 2 1351 .......................................................................................... 1440 D Q E L L D E A L K I A A G A D V I V A A L G E S S E M S G Query_1 1642 GACCAGGAGTTACTGGACGAAGCACTGAAAATTGCAGCCGGCGCCGATGTTATCGTAGCTGCCCTGGGAGAATCTTCTGAAATGAGCGGT 1731 2 1441 .......................................................................................... 1530 E S S S R T E L G L P D V Q H T L L E A L L K T G K P V V L Query_1 1732 GAAAGTTCAAGCCGCACCGAACTCGGCTTGCCCGATGTACAACATACCCTGTTGGAAGCCTTACTGAAAACGGGTAAACCCGTAGTACTA 1821 2 1531 .......................................................................................... 1620 T L F T G R P L T L N W E Q E H V P A I L N V W F G G S E A Query_1 1822 ACCCTCTTTACCGGTCGGCCGTTGACGCTGAACTGGGAACAGGAGCATGTACCTGCCATCTTGAATGTATGGTTCGGAGGCAGTGAAGCG 1911 2 1621 .......................................................................................... 1710 2 1821 ............ 1832 A Y A I G D V L F G D V N P S G K L T M T F P K N V G Q I P Query_1 1912 GCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTCAATCCGAGTGGAAAACTAACCATGACTTTCCCGAAGAATGTAGGCCAGATACCT 2001 2 1711 .......................................................................................... 1800 L F Y N H K N T G R P L A A G K W F E K F R S N Y L D V D N Query_1 2002 TTGTTCTACAATCATAAGAATACCGGTCGGCCACTGGCGGCAGGCAAATGGTTCGAAAAGTTCCGTTCAAACTATCTGGATGTGGATAAC 2091 2 1801 .......................................................................................... 1890 2 1629 ............ 1640 E P L Y P F G Y G L S Y T T F Q Y S D I A L S T P T L G K D Query_1 2092 GAACCGCTGTATCCCTTCGGTTATGGATTGTCGTATACCACTTTCCAGTACAGTGACATTGCATTGAGCACACCGACATTGGGAAAAGAT 2181 2 1891 .......................................................................................... 1980 G S V T A V V T V T N T G K H D G A E V V Q L Y I R D L V G Query_1 2182 GGTTCCGTTACAGCCGTAGTCACCGTCACCAATACTGGTAAACATGACGGTGCGGAAGTAGTTCAACTCTATATCCGCGACCTCGTAGGA 2271 2 1981 .......................................................................................... 2070 S I T R P V R E L K G F N K I F L R A G E S K T V S F T I T Query_1 2272 AGTATCACCCGCCCTGTACGCGAGTTGAAAGGTTTCAATAAAATCTTCCTTCGCGCCGGAGAAAGCAAAACGGTATCATTCACTATCACG 2361 2 2071 .......................................................................................... 2160 2 2166 ...... 2161 R D L L R F Y D Y D L N Y V A E P G D F D I M I G G N S Q A Query_1 2362 CGTGATCTGCTTCGCTTCTATGATTACGACCTGAACTACGTAGCCGAACCGGGTGACTTTGACATCATGATCGGTGGAAACAGCCAGGCT 2451 2 2161 .......................................................................................... 2250 2 2160 ...... 2155 V K T A K L T L Query_1 2452 GTGAAGACGGCGAAGTTGACACTT 2475 2 2251 ........................ 2274 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5954468 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:06 PM Number of letters in database: 2,284 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115038.114 (APC115038.26-783.P(785).F293A.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVTGDITTGQAKSSDVAQKIEKGLVGGLFNLKGVDRILEVQKLAVEKSRLGIPLLFGMDVIHGYETIFPIPLGLSCTWDMAAIEKSARIAAIEASADGISWTFSPMVDISRDPRWGRVSEGSGEDPFLGGAIAQAMVYGYQGANLQDQLHRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEFMYPYEAAVEAGVGSVMASFNEIDGIPATGNKWLLSDLLRGQWGFEGFVVTDFTGISEMIEHGVGDLQTVSALALNAGVDMDMVSEGFVGTLMKSIKEGKVRMGTLNTACRRILEAKYKLGLFDNPYKYCDVNRPKRDIFTKEHRDAARKIAGESFVLLKNAPATAQPLAAHSSSPVTASPVLPLKKQGTVAVIGPLGNTRSNMPGTWSVAARLNDYPSLYEGLKEMMAGKVNITYAKGSNLIGDAAYEERATMFGRSLNRDNRTDQELLDEALKIAAGADVIVAALGESSEMSGESSSRTELGLPDVQHTLLEALLKTGKPVVLTLFTGRPLTLNWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLAAGKWFEKFRSNYLDVDNEPLYPFGYGLSYTTFQYSDIALSTPTLGKDGSVTAVVTVTNTGKHDGAEVVQLYIRDLVGSITRPVRELKGFNKIFLRAGESKTVSFTITRDLLRFYDYDLNYVAEPGDFDIMIGGNSQAVKTAKLTL
AAGTCACCGCAAGACATGGATCGCTTCATCGACGCACTGATGAAGAAGATGACCGTGGAAGAGAAAATCGGACAATTGAACCTACCCGTCACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCACAAAAGATTGAAAAAGGATTGGTGGGCGGACTCTTCAACCTAAAAGGTGTAGACCGTATTCTTGAAGTGCAAAAGCTGGCAGTAGAGAAATCACGCCTCGGTATTCCCCTGCTGTTCGGCATGGATGTGATACATGGCTACGAAACCATCTTCCCCATTCCATTGGGATTGTCCTGCACCTGGGATATGGCGGCTATCGAGAAATCCGCCCGTATTGCAGCCATCGAAGCAAGTGCCGATGGCATTTCCTGGACATTCAGTCCGATGGTAGACATCAGTCGCGACCCACGTTGGGGACGTGTCAGCGAGGGCTCGGGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTATACGGATACCAGGGTGCCAATCTGCAAGACCAATTGCACCGCAACGATGAAATCATGGCTTGCGTAAAACACTTTGCATTGTATGGAGCCGGAGAAGCCGGACGCGACTATAATACAGTAGATATGAGCCGCAACCGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAGGCCGGAGTGGGTAGTGTGATGGCGTCATTCAATGAAATAGACGGTATTCCCGCCACCGGAAACAAATGGCTGCTGAGCGATTTGCTGCGTGGCCAGTGGGGCTTCGAAGGGTTTGTGGTAACGGACTTCACAGGCATTTCAGAGATGATAGAGCATGGTGTCGGCGACTTGCAAACCGTCAGTGCACTCGCTCTTAATGCAGGGGTGGACATGGATATGGTAAGTGAGGGCTTCGTCGGTACACTGATGAAATCAATTAAAGAAGGAAAAGTAAGAATGGGCACGTTGAATACAGCCTGCCGCCGGATATTGGAAGCGAAATACAAGCTGGGACTGTTTGACAATCCTTATAAATACTGCGACGTGAACCGTCCGAAGCGGGATATCTTCACAAAAGAGCATCGTGACGCCGCCCGCAAGATTGCCGGCGAAAGTTTTGTTCTTCTGAAGAATGCCCCCGCCACCGCACAGCCACTCGCAGCTCATAGCTCGTCACCCGTAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAAGGTACAGTTGCCGTCATCGGCCCTCTCGGAAATACCCGCAGCAACATGCCGGGCACCTGGAGCGTAGCCGCACGCCTCAACGATTATCCTTCTTTATACGAAGGCTTGAAAGAAATGATGGCAGGCAAGGTGAACATCACCTATGCCAAAGGTAGTAACCTCATCGGCGATGCAGCTTACGAAGAACGTGCCACCATGTTCGGCCGTTCATTGAACCGCGATAATCGCACGGACCAGGAGTTACTGGACGAAGCACTGAAAATTGCAGCCGGCGCCGATGTTATCGTAGCTGCCCTGGGAGAATCTTCTGAAATGAGCGGTGAAAGTTCAAGCCGCACCGAACTCGGCTTGCCCGATGTACAACATACCCTGTTGGAAGCCTTACTGAAAACGGGTAAACCCGTAGTACTAACCCTCTTTACCGGTCGGCCGTTGACGCTGAACTGGGAACAGGAGCATGTACCTGCCATCTTGAATGTATGGTTCGGAGGCAGTGAAGCGGCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTCAATCCGAGTGGAAAACTAACCATGACTTTCCCGAAGAATGTAGGCCAGATACCTTTGTTCTACAATCATAAGAATACCGGTCGGCCACTGGCGGCAGGCAAATGGTTCGAAAAGTTCCGTTCAAACTATCTGGATGTGGATAACGAACCGCTGTATCCCTTCGGTTATGGATTGTCGTATACCACTTTCCAGTACAGTGACATTGCATTGAGCACACCGACATTGGGAAAAGATGGTTCCGTTACAGCCGTAGTCACCGTCACCAATACTGGTAAACATGACGGTGCGGAAGTAGTTCAACTCTATATCCGCGACCTCGTAGGAAGTATCACCCGCCCTGTACGCGAGTTGAAAGGTTTCAATAAAATCTTCCTTCGCGCCGGAGAAAGCAAAACGGTATCATTCACTATCACGCGTGATCTGCTTCGCTTCTATGATTACGACCTGAACTACGTAGCCGAACCGGGTGACTTTGACATCATGATCGGTGGAAACAGCCAGGCTGTGAAGACGGCGAAGTTGACACTT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,616 total letters Query= APC115038.114 Length=2679 Score E Sequences producing significant alignments: (Bits) Value wt 4087 0.0 01E_E1_APC115038.114_E01.seq 1361 0.0 K S P Q D M D R F I D A L M K K M T V E E K I G Q L N L P V Query_1 202 AAGTCACCGCAAGACATGGATCGCTTCATCGACGCACTGATGAAGAAGATGACCGTGGAAGAGAAAATCGGACAATTGAACCTACCCGTC 291 2 1 .......................................................................................... 90 T G D I T T G Q A K S S D V A Q K I E K G L V G G L F N L K Query_1 292 ACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCACAAAAGATTGAAAAAGGATTGGTGGGCGGACTCTTCAACCTAAAA 381 2 91 .......................................................................................... 180 G V D R I L E V Q K L A V E K S R L G I P L L F G M D V I H Query_1 382 GGTGTAGACCGTATTCTTGAAGTGCAAAAGCTGGCAGTAGAGAAATCACGCCTCGGTATTCCCCTGCTGTTCGGCATGGATGTGATACAT 471 2 181 .......................................................................................... 270 G Y E T I F P I P L G L S C T W D M A A I E K S A R I A A I Query_1 472 GGCTACGAAACCATCTTCCCCATTCCATTGGGATTGTCCTGCACCTGGGATATGGCGGCTATCGAGAAATCCGCCCGTATTGCAGCCATC 561 2 271 .......................................................................................... 360 E A S A D G I S W T F S P M V D I S R D P R W G R V S E G S Query_1 562 GAAGCAAGTGCCGATGGCATTTCCTGGACATTCAGTCCGATGGTAGACATCAGTCGCGACCCACGTTGGGGACGTGTCAGCGAGGGCTCG 651 2 361 .......................................................................................... 450 G E D P F L G G A I A Q A M V Y G Y Q G A N L Q D Q L H R N Query_1 652 GGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTATACGGATACCAGGGTGCCAATCTGCAAGACCAATTGCACCGCAAC 741 2 451 .......................................................................................... 540 0 14 ................................................... 64 D E I M A C V K H F A L Y G A G E A G R D Y N T V D M S R N Query_1 742 GATGAAATCATGGCTTGCGTAAAACACTTTGCATTGTATGGAGCCGGAGAAGCCGGACGCGACTATAATACAGTAGATATGAGCCGCAAC 831 2 541 .......................................................................................... 630 0 65 ...TN..................................................................................... 154 R M F N E F M Y P Y E A A V E A G V G S V M A S F N E I D G Query_1 832 CGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAGGCCGGAGTGGGTAGTGTGATGGCGTCATTCAATGAAATAGACGGT 921 2 631 .......................................................................................... 720 0 155 .......................................................................................... 244 I P A T G N K W L L S D L L R G Q W G F E G F V V T D A T G Query_1 922 ATTCCCGCCACCGGAAACAAATGGCTGCTGAGCGATTTGCTGCGTGGCCAGTGGGGCTTCGAAGGGTTTGTGGTAACGGACGCTACAGGC 1011 2 721 .................................................................................TTC...... 810 0 245 .......................................................................................... 334 I S E M I E H G V G D L Q T V S A L A L N A G V D M D M V S Query_1 1012 ATTTCAGAGATGATAGAGCATGGTGTCGGCGACTTGCAAACCGTCAGTGCACTCGCTCTTAATGCAGGGGTGGACATGGATATGGTAAGT 1101 2 811 .......................................................................................... 900 0 335 .......................................................................................... 424 E G F V G T L M K S I K E G K V R M G T L N T A C R R I L E Query_1 1102 GAGGGCTTCGTCGGTACACTGATGAAATCAATTAAAGAAGGAAAAGTAAGAATGGGCACGTTGAATACAGCCTGCCGCCGGATATTGGAA 1191 2 901 .......................................................................................... 990 0 425 .......................................................................................... 514 A K Y K L G L F D N P Y K Y C D V N R P K R D I F T K E H R Query_1 1192 GCGAAATACAAGCTGGGACTGTTTGACAATCCTTATAAATACTGCGACGTGAACCGTCCGAAGCGGGATATCTTCACAAAAGAGCATCGT 1281 2 991 .......................................................................................... 1080 0 515 .......................................................................................... 604 D A A R K I A G E S F V L L K N A P A T A Q P L A A H S S S Query_1 1282 GACGCCGCCCGCAAGATTGCCGGCGAAAGTTTTGTTCTTCTGAAGAATGCCCCCGCCACCGCACAGCCACTCGCAGCTCATAGCTCGTCA 1371 2 1081 .......................................................................................... 1170 0 605 ........................................................................N..........N...... 694 P V T A S P V L P L K K Q G T V A V I G P L G N T R S N Query_1 1372 CCCG-TAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAAGGTACAGTTG-CCGTCATCGGCCCTCTCG-GAAATACCCGCAG-CAACAT 1457 2 1171 ....-.............................................-..................-.............-...... 1256 0 695 ....T......................N..N........G.N........T.....N............N..........N.NT..N... 784 M P G T W S V A A R L N D Y P S L Y E G L K E M M A G Query_1 1458 GCC--GGGCACCTG-GAGC-GTAGCCGCACGCCTCAA-CGATTAT-CCTTCTTTATACGAA--GGCTTG-AAAGAAATGATGG-CAGGC- 1536 2 1257 ...--.........-....-.................-.......-...............--......-.............-.....- 1335 0 785 ...CG..C.N....TN...G...A....N....N...C.A...N.C............CN.AG.....NA...N.........C.....N 874 K V N I T Y A K G S N L I G D A A Y E E R A T M F G R S Query_1 1537 AAGGTG-AACAT-CACC--TATG-CCAAAGGTA-GTAACCTCATCGGCGATGCAGCTTACGAAGAACGTGCCACCATGTTCGGCCGTTCA 1620 2 1336 ......-.....-....--....-.........-........................................................ 1419 0 875 ......N...C.C....CT...NC.....T...TN..... 914 L N R D N R T D Q E L L D E A L K I A A G A D V I V A A L G Query_1 1621 TTGAACCGCGATAATCGCACGGACCAGGAGTTACTGGACGAAGCACTGAAAATTGCAGCCGGCGCCGATGTTATCGTAGCTGCCCTGGGA 1710 2 1420 .......................................................................................... 1509 E S S E M S G E S S S R T E L G L P D V Q H T L L E A L L K Query_1 1711 GAATCTTCTGAAATGAGCGGTGAAAGTTCAAGCCGCACCGAACTCGGCTTGCCCGATGTACAACATACCCTGTTGGAAGCCTTACTGAAA 1800 2 1510 .......................................................................................... 1599 T G K P V V L T L F T G R P L T L N W E Q E H V P A I L N V Query_1 1801 ACGGGTAAACCCGTAGTACTAACCCTCTTTACCGGTCGGCCGTTGACGCTGAACTGGGAACAGGAGCATGTACCTGCCATCTTGAATGTA 1890 2 1600 .......................................................................................... 1689 2 1821 ............ 1832 W F G G S E A A Y A I G D V L F G D V N P S G K L T M T F P Query_1 1891 TGGTTCGGAGGCAGTGAAGCGGCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTCAATCCGAGTGGAAAACTAACCATGACTTTCCCG 1980 2 1690 .......................................................................................... 1779 K N V G Q I P L F Y N H K N T G R P L A A G K W F E K F R S Query_1 1981 AAGAATGTAGGCCAGATACCTTTGTTCTACAATCATAAGAATACCGGTCGGCCACTGGCGGCAGGCAAATGGTTCGAAAAGTTCCGTTCA 2070 2 1780 .......................................................................................... 1869 2 1629 ............ 1640 N Y L D V D N E P L Y P F G Y G L S Y T T F Q Y S D I A L S Query_1 2071 AACTATCTGGATGTGGATAACGAACCGCTGTATCCCTTCGGTTATGGATTGTCGTATACCACTTTCCAGTACAGTGACATTGCATTGAGC 2160 2 1870 .......................................................................................... 1959 T P T L G K D G S V T A V V T V T N T G K H D G A E V V Q L Query_1 2161 ACACCGACATTGGGAAAAGATGGTTCCGTTACAGCCGTAGTCACCGTCACCAATACTGGTAAACATGACGGTGCGGAAGTAGTTCAACTC 2250 2 1960 .......................................................................................... 2049 Y I R D L V G S I T R P V R E L K G F N K I F L R A G E S K Query_1 2251 TATATCCGCGACCTCGTAGGAAGTATCACCCGCCCTGTACGCGAGTTGAAAGGTTTCAATAAAATCTTCCTTCGCGCCGGAGAAAGCAAA 2340 2 2050 .......................................................................................... 2139 T V S F T I T R D L L R F Y D Y D L N Y V A E P G D F D I M Query_1 2341 ACGGTATCATTCACTATCACGCGTGATCTGCTTCGCTTCTATGATTACGACCTGAACTACGTAGCCGAACCGGGTGACTTTGACATCATG 2430 2 2140 .......................................................................................... 2229 2 2166 ............ 2155 I G G N S Q A V K T A K L T L Query_1 2431 ATCGGTGGAAACAGCCAGGCTGTGAAGACGGCGAAGTTGACACTT 2475 2 2230 ............................................. 2274 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 9490030 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:06 PM Number of letters in database: 3,616 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115038.115 (APC115038.26-783.P(785).F646A.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVTGDITTGQAKSSDVAQKIEKGLVGGLFNLKGVDRILEVQKLAVEKSRLGIPLLFGMDVIHGYETIFPIPLGLSCTWDMAAIEKSARIAAIEASADGISWTFSPMVDISRDPRWGRVSEGSGEDPFLGGAIAQAMVYGYQGANLQDQLHRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEFMYPYEAAVEAGVGSVMASFNEIDGIPATGNKWLLSDLLRGQWGFEGFVVTDFTGISEMIEHGVGDLQTVSALALNAGVDMDMVSEGFVGTLMKSIKEGKVRMGTLNTACRRILEAKYKLGLFDNPYKYCDVNRPKRDIFTKEHRDAARKIAGESFVLLKNAPATAQPLAAHSSSPVTASPVLPLKKQGTVAVIGPLGNTRSNMPGTWSVAARLNDYPSLYEGLKEMMAGKVNITYAKGSNLIGDAAYEERATMFGRSLNRDNRTDQELLDEALKIAAGADVIVAALGESSEMSGESSSRTELGLPDVQHTLLEALLKTGKPVVLTLFTGRPLTLNWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLAAGKWFEKFRSNYLDVDNEPLYPFGYGLSYTTFQYSDIALSTPTLGKDGSVTAVVTVTNTGKHDGAEVVQLYIRDLVGSITRPVRELKGFNKIFLRAGESKTVSFTITRDLLRFYDYDLNYVAEPGDFDIMIGGNSQAVKTAKLTL
AAGTCACCGCAAGACATGGATCGCTTCATCGACGCACTGATGAAGAAGATGACCGTGGAAGAGAAAATCGGACAATTGAACCTACCCGTCACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCACAAAAGATTGAAAAAGGATTGGTGGGCGGACTCTTCAACCTAAAAGGTGTAGACCGTATTCTTGAAGTGCAAAAGCTGGCAGTAGAGAAATCACGCCTCGGTATTCCCCTGCTGTTCGGCATGGATGTGATACATGGCTACGAAACCATCTTCCCCATTCCATTGGGATTGTCCTGCACCTGGGATATGGCGGCTATCGAGAAATCCGCCCGTATTGCAGCCATCGAAGCAAGTGCCGATGGCATTTCCTGGACATTCAGTCCGATGGTAGACATCAGTCGCGACCCACGTTGGGGACGTGTCAGCGAGGGCTCGGGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTATACGGATACCAGGGTGCCAATCTGCAAGACCAATTGCACCGCAACGATGAAATCATGGCTTGCGTAAAACACTTTGCATTGTATGGAGCCGGAGAAGCCGGACGCGACTATAATACAGTAGATATGAGCCGCAACCGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAGGCCGGAGTGGGTAGTGTGATGGCGTCATTCAATGAAATAGACGGTATTCCCGCCACCGGAAACAAATGGCTGCTGAGCGATTTGCTGCGTGGCCAGTGGGGCTTCGAAGGGTTTGTGGTAACGGACTTCACAGGCATTTCAGAGATGATAGAGCATGGTGTCGGCGACTTGCAAACCGTCAGTGCACTCGCTCTTAATGCAGGGGTGGACATGGATATGGTAAGTGAGGGCTTCGTCGGTACACTGATGAAATCAATTAAAGAAGGAAAAGTAAGAATGGGCACGTTGAATACAGCCTGCCGCCGGATATTGGAAGCGAAATACAAGCTGGGACTGTTTGACAATCCTTATAAATACTGCGACGTGAACCGTCCGAAGCGGGATATCTTCACAAAAGAGCATCGTGACGCCGCCCGCAAGATTGCCGGCGAAAGTTTTGTTCTTCTGAAGAATGCCCCCGCCACCGCACAGCCACTCGCAGCTCATAGCTCGTCACCCGTAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAAGGTACAGTTGCCGTCATCGGCCCTCTCGGAAATACCCGCAGCAACATGCCGGGCACCTGGAGCGTAGCCGCACGCCTCAACGATTATCCTTCTTTATACGAAGGCTTGAAAGAAATGATGGCAGGCAAGGTGAACATCACCTATGCCAAAGGTAGTAACCTCATCGGCGATGCAGCTTACGAAGAACGTGCCACCATGTTCGGCCGTTCATTGAACCGCGATAATCGCACGGACCAGGAGTTACTGGACGAAGCACTGAAAATTGCAGCCGGCGCCGATGTTATCGTAGCTGCCCTGGGAGAATCTTCTGAAATGAGCGGTGAAAGTTCAAGCCGCACCGAACTCGGCTTGCCCGATGTACAACATACCCTGTTGGAAGCCTTACTGAAAACGGGTAAACCCGTAGTACTAACCCTCTTTACCGGTCGGCCGTTGACGCTGAACTGGGAACAGGAGCATGTACCTGCCATCTTGAATGTATGGTTCGGAGGCAGTGAAGCGGCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTCAATCCGAGTGGAAAACTAACCATGACTTTCCCGAAGAATGTAGGCCAGATACCTTTGTTCTACAATCATAAGAATACCGGTCGGCCACTGGCGGCAGGCAAATGGTTCGAAAAGTTCCGTTCAAACTATCTGGATGTGGATAACGAACCGCTGTATCCCTTCGGTTATGGATTGTCGTATACCACTTTCCAGTACAGTGACATTGCATTGAGCACACCGACATTGGGAAAAGATGGTTCCGTTACAGCCGTAGTCACCGTCACCAATACTGGTAAACATGACGGTGCGGAAGTAGTTCAACTCTATATCCGCGACCTCGTAGGAAGTATCACCCGCCCTGTACGCGAGTTGAAAGGTTTCAATAAAATCTTCCTTCGCGCCGGAGAAAGCAAAACGGTATCATTCACTATCACGCGTGATCTGCTTCGCTTCTATGATTACGACCTGAACTACGTAGCCGAACCGGGTGACTTTGACATCATGATCGGTGGAAACAGCCAGGCTGTGAAGACGGCGAAGTTGACACTT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,432 total letters Query= APC115038.115 Length=2679 Score E Sequences producing significant alignments: (Bits) Value wt 4087 0.0 01F_F1_APC115038.115_F01.seq 399 4e-114 K S P Q D M D R F I D A L M K K M T V E E K I G Q L N L P V Query_1 202 AAGTCACCGCAAGACATGGATCGCTTCATCGACGCACTGATGAAGAAGATGACCGTGGAAGAGAAAATCGGACAATTGAACCTACCCGTC 291 2 1 .......................................................................................... 90 T G D I T T G Q A K S S D V A Q K I E K G L V G G L F N L K Query_1 292 ACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCACAAAAGATTGAAAAAGGATTGGTGGGCGGACTCTTCAACCTAAAA 381 2 91 .......................................................................................... 180 G V D R I L E V Q K L A V E K S R L G I P L L F G M D V I H Query_1 382 GGTGTAGACCGTATTCTTGAAGTGCAAAAGCTGGCAGTAGAGAAATCACGCCTCGGTATTCCCCTGCTGTTCGGCATGGATGTGATACAT 471 2 181 .......................................................................................... 270 G Y E T I F P I P L G L S C T W D M A A I E K S A R I A A I Query_1 472 GGCTACGAAACCATCTTCCCCATTCCATTGGGATTGTCCTGCACCTGGGATATGGCGGCTATCGAGAAATCCGCCCGTATTGCAGCCATC 561 2 271 .......................................................................................... 360 E A S A D G I S W T F S P M V D I S R D P R W G R V S E G S Query_1 562 GAAGCAAGTGCCGATGGCATTTCCTGGACATTCAGTCCGATGGTAGACATCAGTCGCGACCCACGTTGGGGACGTGTCAGCGAGGGCTCG 651 2 361 .......................................................................................... 450 G E D P F L G G A I A Q A M V Y G Y Q G A N L Q D Q L H R N Query_1 652 GGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTATACGGATACCAGGGTGCCAATCTGCAAGACCAATTGCACCGCAAC 741 2 451 .......................................................................................... 540 D E I M A C V K H F A L Y G A G E A G R D Y N T V D M S R N Query_1 742 GATGAAATCATGGCTTGCGTAAAACACTTTGCATTGTATGGAGCCGGAGAAGCCGGACGCGACTATAATACAGTAGATATGAGCCGCAAC 831 2 541 .......................................................................................... 630 R M F N E F M Y P Y E A A V E A G V G S V M A S F N E I D G Query_1 832 CGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAGGCCGGAGTGGGTAGTGTGATGGCGTCATTCAATGAAATAGACGGT 921 2 631 .......................................................................................... 720 I P A T G N K W L L S D L L R G Q W G F E G F V V T D F T G Query_1 922 ATTCCCGCCACCGGAAACAAATGGCTGCTGAGCGATTTGCTGCGTGGCCAGTGGGGCTTCGAAGGGTTTGTGGTAACGGACTTCACAGGC 1011 2 721 .......................................................................................... 810 I S E M I E H G V G D L Q T V S A L A L N A G V D M D M V S Query_1 1012 ATTTCAGAGATGATAGAGCATGGTGTCGGCGACTTGCAAACCGTCAGTGCACTCGCTCTTAATGCAGGGGTGGACATGGATATGGTAAGT 1101 2 811 .......................................................................................... 900 E G F V G T L M K S I K E G K V R M G T L N T A C R R I L E Query_1 1102 GAGGGCTTCGTCGGTACACTGATGAAATCAATTAAAGAAGGAAAAGTAAGAATGGGCACGTTGAATACAGCCTGCCGCCGGATATTGGAA 1191 2 901 .......................................................................................... 990 A K Y K L G L F D N P Y K Y C D V N R P K R D I F T K E H R Query_1 1192 GCGAAATACAAGCTGGGACTGTTTGACAATCCTTATAAATACTGCGACGTGAACCGTCCGAAGCGGGATATCTTCACAAAAGAGCATCGT 1281 2 991 .......................................................................................... 1080 D A A R K I A G E S F V L L K N A P A T A Q P L A A H S S S Query_1 1282 GACGCCGCCCGCAAGATTGCCGGCGAAAGTTTTGTTCTTCTGAAGAATGCCCCCGCCACCGCACAGCCACTCGCAGCTCATAGCTCGTCA 1371 2 1081 .......................................................................................... 1170 P V T A S P V L P L K K Q G T V A V I G P L G N T R S N M P Query_1 1372 CCCGTAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAAGGTACAGTTGCCGTCATCGGCCCTCTCGGAAATACCCGCAGCAACATGCCG 1461 2 1171 .......................................................................................... 1260 G T W S V A A R L N D Y P S L Y E G L K E M M A G K V N I T Query_1 1462 GGCACCTGGAGCGTAGCCGCACGCCTCAACGATTATCCTTCTTTATACGAAGGCTTGAAAGAAATGATGGCAGGCAAGGTGAACATCACC 1551 2 1261 .......................................................................................... 1350 Y A K G S N L I G D A A Y E E R A T M F G R S L N R D N R T Query_1 1552 TATGCCAAAGGTAGTAACCTCATCGGCGATGCAGCTTACGAAGAACGTGCCACCATGTTCGGCCGTTCATTGAACCGCGATAATCGCACG 1641 2 1351 .......................................................................................... 1440 D Q E L L D E A L K I A A G A D V I V A A L G E S S E M S G Query_1 1642 GACCAGGAGTTACTGGACGAAGCACTGAAAATTGCAGCCGGCGCCGATGTTATCGTAGCTGCCCTGGGAGAATCTTCTGAAATGAGCGGT 1731 2 1441 .......................................................................................... 1530 0 307 .......NN.N.NN....CAN...A.........N..CGNC......A.N..N....AAANTTTT.....C-. 378 E S S S R T E L G L P D V Q H T L L E A L L K T G K P V V L Query_1 1732 GAAAGTTCAAGCCGCACCGAACTCGGCTTGCCCGATGTACAACATACCCTGTTGGAAGCCTTACTGAAAACGGGTAAACCCGTAGTACTA 1821 2 1531 .......................................................................................... 1620 0 379 ...NAA.GN......N..N.N.....N.............N...............N-...............NN...N..NCTN..N.N 467 T L F T G R P L T L N W E Q E H V P A I L N V W F G G S E A Query_1 1822 ACCCTCTTTACCGGTCGGCCGTTGACGCTGAACTGGGAACAGGAGCATGTACCTGCCATCTTGAATGTATGGTTCGGAGGCAGTGAAGCG 1911 2 1621 .......................................................................................... 1710 2 1821 ............ 1832 0 468 ..................N.......N..T....NTN...TN..C....N.....N................A.....N...NN.T.TN. 557 A Y A I G D V L F G D V N P S G K L T M T F P K N V G Q I P Query_1 1912 GCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTCAATCCGAGTGGAAAACTAACCATGACTTTCCCGAAGAATGTAGGCCAGATACCT 2001 2 1711 .......................................................................................... 1800 0 558 N..............N.....NN.N....N...N.-...N........C.............................T....N...NT. 646 L F Y N H K N T G R P L A A G K W F E K A R S N Y L D V D N Query_1 2002 TTGTTCTACAATCATAAGAATACCGGTCGGCCACTGGCGGCAGGCAAATGGTTCGAAAAGGCTCGTTCAAACTATCTGGATGTGGATAAC 2091 2 1801 ............................................................TTC........................... 1890 2 1629 ............ 1640 0 647 ......-N.C......NT..N...NT..N.N..TAAAANAA.NN.G....TN..-T.N..NN.A.A..N.T.......N.-.G.C..... 733 E P L Y P F G Y G L S Y T T F Q Y S D I A L S T P T L G K Query_1 2092 GAACCGCTGTATCCCTTCGGTTATGGATTGTCGTATACCACTTTCCAGTACAGTGACATTGCAT-TGAGCACACCGACATTGGGAAAAGA 2180 2 1891 ................................................................-......................... 1979 0 734 N.....N...GN.G....C.C.N....N..N.C..N.....-.N..C.A..GN....N..N...CNN.NTN.N...TN.....N...NA. 822 D G S V T A V V T V T N T G K H D G A E V V Q L Y I R D L V Query_1 2181 TGGTT-CCGTTACAGCCGTAGTCACCGTCACCAATACTGGTAAACATGACGGTGCGGAAGTAGTTCAACTCTATATCCGCGACCTCGTAG 2269 2 1980 .....-.................................................................................... 2068 0 823 ...NNC.....N.NT...N.CG.T...A.N..NN.N..............NN.........N..........TN........N.NNN.NC 912 G S I T R P V R E L K G F N K I F L R A G E S K T V S F T I Query_1 2270 GAAGTATCACCCGCCCTGTACGCGAGTTGAAAGGTTTCAATAAAATCTTCCTTCGCGCCGGAGAAAGCAAAACGGTATCATTCACTATCA 2359 2 2069 .......................................................................................... 2158 2 2166 .... 2163 0 913 ....-C.T........C...NN..N.-..T....G..AC..NG...T..T.A.... 966 T R D L L R F Y D Y D L N Y V A E P G D F D I M I G G N S Q Query_1 2360 CGCGTGATCTGCTTCGCTTCTATGATTACGACCTGAACTACGTAGCCGAACCGGGTGACTTTGACATCATGATCGGTGGAAACAGCCAGG 2449 2 2159 .......................................................................................... 2248 2 2162 ........ 2155 A V K T A K L T L Query_1 2450 CTGTGAAGACGGCGAAGTTGACACTT 2475 2 2249 .......................... 2274 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 9000222 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:06 PM Number of letters in database: 3,432 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115038.116 (APC115038.26-783.P(785).W448F.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVTGDITTGQAKSSDVAQKIEKGLVGGLFNLKGVDRILEVQKLAVEKSRLGIPLLFGMDVIHGYETIFPIPLGLSCTWDMAAIEKSARIAAIEASADGISWTFSPMVDISRDPRWGRVSEGSGEDPFLGGAIAQAMVYGYQGANLQDQLHRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEFMYPYEAAVEAGVGSVMASFNEIDGIPATGNKWLLSDLLRGQWGFEGFVVTDFTGISEMIEHGVGDLQTVSALALNAGVDMDMVSEGFVGTLMKSIKEGKVRMGTLNTACRRILEAKYKLGLFDNPYKYCDVNRPKRDIFTKEHRDAARKIAGESFVLLKNAPATAQPLAAHSSSPVTASPVLPLKKQGTVAVIGPLGNTRSNMPGTWSVAARLNDYPSLYEGLKEMMAGKVNITYAKGSNLIGDAAYEERATMFGRSLNRDNRTDQELLDEALKIAAGADVIVAALGESSEMSGESSSRTELGLPDVQHTLLEALLKTGKPVVLTLFTGRPLTLNWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLAAGKWFEKFRSNYLDVDNEPLYPFGYGLSYTTFQYSDIALSTPTLGKDGSVTAVVTVTNTGKHDGAEVVQLYIRDLVGSITRPVRELKGFNKIFLRAGESKTVSFTITRDLLRFYDYDLNYVAEPGDFDIMIGGNSQAVKTAKLTL
AAGTCACCGCAAGACATGGATCGCTTCATCGACGCACTGATGAAGAAGATGACCGTGGAAGAGAAAATCGGACAATTGAACCTACCCGTCACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCACAAAAGATTGAAAAAGGATTGGTGGGCGGACTCTTCAACCTAAAAGGTGTAGACCGTATTCTTGAAGTGCAAAAGCTGGCAGTAGAGAAATCACGCCTCGGTATTCCCCTGCTGTTCGGCATGGATGTGATACATGGCTACGAAACCATCTTCCCCATTCCATTGGGATTGTCCTGCACCTGGGATATGGCGGCTATCGAGAAATCCGCCCGTATTGCAGCCATCGAAGCAAGTGCCGATGGCATTTCCTGGACATTCAGTCCGATGGTAGACATCAGTCGCGACCCACGTTGGGGACGTGTCAGCGAGGGCTCGGGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTATACGGATACCAGGGTGCCAATCTGCAAGACCAATTGCACCGCAACGATGAAATCATGGCTTGCGTAAAACACTTTGCATTGTATGGAGCCGGAGAAGCCGGACGCGACTATAATACAGTAGATATGAGCCGCAACCGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAGGCCGGAGTGGGTAGTGTGATGGCGTCATTCAATGAAATAGACGGTATTCCCGCCACCGGAAACAAATGGCTGCTGAGCGATTTGCTGCGTGGCCAGTGGGGCTTCGAAGGGTTTGTGGTAACGGACTTCACAGGCATTTCAGAGATGATAGAGCATGGTGTCGGCGACTTGCAAACCGTCAGTGCACTCGCTCTTAATGCAGGGGTGGACATGGATATGGTAAGTGAGGGCTTCGTCGGTACACTGATGAAATCAATTAAAGAAGGAAAAGTAAGAATGGGCACGTTGAATACAGCCTGCCGCCGGATATTGGAAGCGAAATACAAGCTGGGACTGTTTGACAATCCTTATAAATACTGCGACGTGAACCGTCCGAAGCGGGATATCTTCACAAAAGAGCATCGTGACGCCGCCCGCAAGATTGCCGGCGAAAGTTTTGTTCTTCTGAAGAATGCCCCCGCCACCGCACAGCCACTCGCAGCTCATAGCTCGTCACCCGTAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAAGGTACAGTTGCCGTCATCGGCCCTCTCGGAAATACCCGCAGCAACATGCCGGGCACCTGGAGCGTAGCCGCACGCCTCAACGATTATCCTTCTTTATACGAAGGCTTGAAAGAAATGATGGCAGGCAAGGTGAACATCACCTATGCCAAAGGTAGTAACCTCATCGGCGATGCAGCTTACGAAGAACGTGCCACCATGTTCGGCCGTTCATTGAACCGCGATAATCGCACGGACCAGGAGTTACTGGACGAAGCACTGAAAATTGCAGCCGGCGCCGATGTTATCGTAGCTGCCCTGGGAGAATCTTCTGAAATGAGCGGTGAAAGTTCAAGCCGCACCGAACTCGGCTTGCCCGATGTACAACATACCCTGTTGGAAGCCTTACTGAAAACGGGTAAACCCGTAGTACTAACCCTCTTTACCGGTCGGCCGTTGACGCTGAACTGGGAACAGGAGCATGTACCTGCCATCTTGAATGTATGGTTCGGAGGCAGTGAAGCGGCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTCAATCCGAGTGGAAAACTAACCATGACTTTCCCGAAGAATGTAGGCCAGATACCTTTGTTCTACAATCATAAGAATACCGGTCGGCCACTGGCGGCAGGCAAATGGTTCGAAAAGTTCCGTTCAAACTATCTGGATGTGGATAACGAACCGCTGTATCCCTTCGGTTATGGATTGTCGTATACCACTTTCCAGTACAGTGACATTGCATTGAGCACACCGACATTGGGAAAAGATGGTTCCGTTACAGCCGTAGTCACCGTCACCAATACTGGTAAACATGACGGTGCGGAAGTAGTTCAACTCTATATCCGCGACCTCGTAGGAAGTATCACCCGCCCTGTACGCGAGTTGAAAGGTTTCAATAAAATCTTCCTTCGCGCCGGAGAAAGCAAAACGGTATCATTCACTATCACGCGTGATCTGCTTCGCTTCTATGATTACGACCTGAACTACGTAGCCGAACCGGGTGACTTTGACATCATGATCGGTGGAAACAGCCAGGCTGTGAAGACGGCGAAGTTGACACTT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,616 total letters Query= APC115038.116 Length=2679 Score E Sequences producing significant alignments: (Bits) Value wt 4093 0.0 07G_G1_APC115038.116_G01.seq 1290 0.0 01G_G1_APC115038.116_G01.seq 322 1e-90 K S P Q D M D R F I D A L M K K M T V E E K I G Q L N L P V Query_1 202 AAGTCACCGCAAGACATGGATCGCTTCATCGACGCACTGATGAAGAAGATGACCGTGGAAGAGAAAATCGGACAATTGAACCTACCCGTC 291 2 1 .......................................................................................... 90 T G D I T T G Q A K S S D V A Q K I E K G L V G G L F N L K Query_1 292 ACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCACAAAAGATTGAAAAAGGATTGGTGGGCGGACTCTTCAACCTAAAA 381 2 91 .......................................................................................... 180 G V D R I L E V Q K L A V E K S R L G I P L L F G M D V I H Query_1 382 GGTGTAGACCGTATTCTTGAAGTGCAAAAGCTGGCAGTAGAGAAATCACGCCTCGGTATTCCCCTGCTGTTCGGCATGGATGTGATACAT 471 2 181 .......................................................................................... 270 G Y E T I F P I P L G L S C T W D M A A I E K S A R I A A I Query_1 472 GGCTACGAAACCATCTTCCCCATTCCATTGGGATTGTCCTGCACCTGGGATATGGCGGCTATCGAGAAATCCGCCCGTATTGCAGCCATC 561 2 271 .......................................................................................... 360 E A S A D G I S W T F S P M V D I S R D P R W G R V S E G S Query_1 562 GAAGCAAGTGCCGATGGCATTTCCTGGACATTCAGTCCGATGGTAGACATCAGTCGCGACCCACGTTGGGGACGTGTCAGCGAGGGCTCG 651 2 361 .......................................................................................... 450 G E D P F L G G A I A Q A M V Y G Y Q G A N L Q D Q L H R N Query_1 652 GGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTATACGGATACCAGGGTGCCAATCTGCAAGACCAATTGCACCGCAAC 741 2 451 .......................................................................................... 540 D E I M A C V K H F A L Y G A G E A G R D Y N T V D M S R N Query_1 742 GATGAAATCATGGCTTGCGTAAAACACTTTGCATTGTATGGAGCCGGAGAAGCCGGACGCGACTATAATACAGTAGATATGAGCCGCAAC 831 2 541 .......................................................................................... 630 R M F N E F M Y P Y E A A V E A G V G S V M A S F N E I D G Query_1 832 CGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAGGCCGGAGTGGGTAGTGTGATGGCGTCATTCAATGAAATAGACGGT 921 2 631 .......................................................................................... 720 1 910 .... 907 I P A T G N K W L L S D L L R G Q W G F E G F V V Query_1 922 ATTCCC-GCCACCGGAAA--CAAATGGCT--GCTGAGCGATTTGCTG-CGTGG--CCAGTGGGGCTTC-GAAGGGTTT--GTGGTAAC-- 998 2 721 ......-...........--.........--................-.....--.............-.........--........-- 797 1 906 N....NN........G..GA.....T.A.TGNN.NN........AN.G.A...TC........NN...AN.....N..TN.N.....NCG 817 0 40 ....-C.N...N..--.N..N..N-- 60 T D F T G I S E M I E H G V G D L Q T V S A L A L N A G Query_1 999 GGACTTCACA-GGCATTTC-AGA-GATGATA-GAGCATGG-TGTC-GGCGACTTGC-AAACCGTCAG-TGCACTCGCTCTTAATGCAGGG 1080 2 798 ..........-........-...-.......-........-....-..........-..........-...................... 879 1 816 ...T......A.......NN...AN...NN.A........GNN..N...N......C....N.....N......N...N........... 727 0 61 A.CN.N..GN-T..-....-NN.-..N.C.C-N..NG..N-N..N-.C.NCN....-..CNG.GNC.-....N.GTNN..N......T.. 141 V D M D M V S E G F V G T L M K S I K E G K V R M G T L Query_1 1081 G-TGGACATGGATATGGTAAGTG-AGGGCTT-CGTCGG-TACACTGATGAAATCAATTAAAGAAGGAAAAGTAAGAATGGGCACGTTGAA 1166 2 880 .-.....................-.......-......-................................................... 965 1 726 .G............N......NNN....N..T......N...TN.............................................. 637 0 142 .-N........N.N.. 156 N T A C R R I L E A K Y K L G L F D N P Y K Y C D V N R P K Query_1 1167 T-ACAGCCTGCCGCCGGATATTGGAAGCGAAATACAAGCTGGGACTGTTTGACAATCCTTATAAATACTGCGACGTGAACCGTCCGAAGC 1255 2 966 .-........................................................................................ 1054 1 636 .N..........N...............................N............................................. 547 R D I F T K E H R D A A R K I A G E S F V L L K N A P A T A Query_1 1256 GGGATATCTTCACAAAAGAGCATCGTGACGCCGCCCGCAAGATT-GCCGGCGAAAGTTTTGTTCTTCTGAAGAATGCCCCCGCCACCGCA 1344 2 1055 ............................................-............................................. 1143 1 546 ............................................T..................N.......................... 457 0 373 ..............C.......-.....A. 401 Q P L A A H S S S P V T A S P V L P L K K Q G T V A V I G P Query_1 1345 CAGCCACTCGCAGCTCATAGCTCGTCACCCGTAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAAGGTACAGTTGCCGTCATCGGCCCT 1434 2 1144 .......................................................................................... 1233 1 456 ...................N...................................................................... 367 0 402 . 402 L G N T R S N M P G T F S V A A R L N D Y P S L Y E G L K E Query_1 1435 CTCGGAAATACCCGCAGCAACATGCCGGGCACCTTTAGCGTAGCCGCACGCCTCAACGATTATCCTTCTTTATACGAAGGCTTGAAAGAA 1524 2 1234 ..................................GG...................................................... 1323 1 366 .......................................................................................... 277 M M A G K V N I T Y A K G S N L I G D A A Y E E R A T M F G Query_1 1525 ATGATGGCAGGCAAGGTGAACATCACCTATGCCAAAGGTAGTAACCTCATCGGCGATGCAGCTTACGAAGAACGTGCCACCATGTTCGGC 1614 2 1324 .......................................................................................... 1413 1 276 .......................................................................................... 187 R S L N R D N R T D Q E L L D E A L K I A A G A D V I V A A Query_1 1615 CGTTCATTGAACCGCGATAATCGCACGGACCAGGAGTTACTGGACGAAGCACTGAAAATTGCAGCCGGCGCCGATGTTATCGTAGCTGCC 1704 2 1414 .......................................................................................... 1503 1 186 .......................................................................................... 97 0 302 ...N..N.........AN.......CNN...........GNCN.CN.C..... 354 L G E S S E M S G E S S S R T E L G L P D V Q H T L L E A Query_1 1705 CTGGGAGAATCTTCTGAAATGAGC--GGTGAAAGTTCAAGCCGCACCGAACTCGGCTTGCCCGATGTACAACATACCCTGTTGGAAGCCT 1792 2 1504 ........................--................................................................ 1591 1 96 ........................--................................................N............... 9 0 355 .A....N.......N..NG.TCTTCT.AA...C.CC.---...............N...........N.............GN....... 441 L L K T G K P V V L T L F T G R P L T L N W E Q E H V P A I Query_1 1793 TACTGAAAACGGGTAAACCCGTAGTACTAACCCTCTTTACCGGTCGGCCGTTGACGCTGAACTGGGAACAGGAGCATGTACCTGCCATCT 1882 2 1592 .......................................................................................... 1681 2 1821 ............ 1832 1 8 ...G.... 1 0 442 .........N..N.........CN-...............N.........N....N.........N....N.......N.......NA.N 530 L N V W F G G S E A A Y A I G D V L F G D V N P S G K L T M Query_1 1883 TGAATGTATGGTTCGGAGGCAGTGAAGCGGCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTCAATCCGAGTGGAAAACTAACCATGA 1972 2 1682 .......................................................................................... 1771 0 531 .N.....N...N.N.N.A.NNA..N.N.NNN...N....N.NN.A.....NN....NNN...NN..N.......-.NNCN...N...... 619 T F P K N V G Q I P L F Y N H K N T G R P L A A G K W F E K Query_1 1973 CTTTCCCGAAGAATGTAGGCCAGATACCTTTGTTCTACAATCATAAGAATACCGGTCGGCCACTGGCGGCAGGCAAATGGTTCGAAAAGT 2062 2 1772 .......................................................................................... 1861 2 1629 ............ 1640 0 620 ....N.......C..N.T....C.........C.....-N..N..........TNN.........A.NNNTN..GN....G...G.NT.N 708 F R S N Y L D V D N E P L Y P F G Y G L S Y T T F Q Y S D I Query_1 2063 TCCGTTCAAACTATCTGGATGTGGATAACGAACCGCTGTATCCCTTCGGTTATGGATTGTCGTATACCACTTTCCAGTACAGTGACATTG 2152 2 1862 .......................................................................................... 1951 0 709 N...A....T....AN.......N.N... 737 A L S T P T L G K D G S V T A V V T V T N T G K H D G A E V Query_1 2153 CATTGAGCACACCGACATTGGGAAAAGATGGTTCCGTTACAGCCGTAGTCACCGTCACCAATACTGGTAAACATGACGGTGCGGAAGTAG 2242 2 1952 .......................................................................................... 2041 V Q L Y I R D L V G S I T R P V R E L K G F N K I F L R A G Query_1 2243 TTCAACTCTATATCCGCGACCTCGTAGGAAGTATCACCCGCCCTGTACGCGAGTTGAAAGGTTTCAATAAAATCTTCCTTCGCGCCGGAG 2332 2 2042 .......................................................................................... 2131 E S K T V S F T I T R D L L R F Y D Y D L N Y V A E P G D F Query_1 2333 AAAGCAAAACGGTATCATTCACTATCACGCGTGATCTGCTTCGCTTCTATGATTACGACCTGAACTACGTAGCCGAACCGGGTGACTTTG 2422 2 2132 .......................................................................................... 2221 2 2166 ............ 2155 D I M I G G N S Q A V K T A K L T L Query_1 2423 ACATCATGATCGGTGGAAACAGCCAGGCTGTGAAGACGGCGAAGTTGACACTT 2475 2 2222 ..................................................... 2274 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12152030 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:06 PM Number of letters in database: 4,616 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115038.118 (APC115038.26-783.P(785).F293W.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVTGDITTGQAKSSDVAQKIEKGLVGGLFNLKGVDRILEVQKLAVEKSRLGIPLLFGMDVIHGYETIFPIPLGLSCTWDMAAIEKSARIAAIEASADGISWTFSPMVDISRDPRWGRVSEGSGEDPFLGGAIAQAMVYGYQGANLQDQLHRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEFMYPYEAAVEAGVGSVMASFNEIDGIPATGNKWLLSDLLRGQWGFEGFVVTDFTGISEMIEHGVGDLQTVSALALNAGVDMDMVSEGFVGTLMKSIKEGKVRMGTLNTACRRILEAKYKLGLFDNPYKYCDVNRPKRDIFTKEHRDAARKIAGESFVLLKNAPATAQPLAAHSSSPVTASPVLPLKKQGTVAVIGPLGNTRSNMPGTWSVAARLNDYPSLYEGLKEMMAGKVNITYAKGSNLIGDAAYEERATMFGRSLNRDNRTDQELLDEALKIAAGADVIVAALGESSEMSGESSSRTELGLPDVQHTLLEALLKTGKPVVLTLFTGRPLTLNWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLAAGKWFEKFRSNYLDVDNEPLYPFGYGLSYTTFQYSDIALSTPTLGKDGSVTAVVTVTNTGKHDGAEVVQLYIRDLVGSITRPVRELKGFNKIFLRAGESKTVSFTITRDLLRFYDYDLNYVAEPGDFDIMIGGNSQAVKTAKLTL
AAGTCACCGCAAGACATGGATCGCTTCATCGACGCACTGATGAAGAAGATGACCGTGGAAGAGAAAATCGGACAATTGAACCTACCCGTCACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCACAAAAGATTGAAAAAGGATTGGTGGGCGGACTCTTCAACCTAAAAGGTGTAGACCGTATTCTTGAAGTGCAAAAGCTGGCAGTAGAGAAATCACGCCTCGGTATTCCCCTGCTGTTCGGCATGGATGTGATACATGGCTACGAAACCATCTTCCCCATTCCATTGGGATTGTCCTGCACCTGGGATATGGCGGCTATCGAGAAATCCGCCCGTATTGCAGCCATCGAAGCAAGTGCCGATGGCATTTCCTGGACATTCAGTCCGATGGTAGACATCAGTCGCGACCCACGTTGGGGACGTGTCAGCGAGGGCTCGGGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTATACGGATACCAGGGTGCCAATCTGCAAGACCAATTGCACCGCAACGATGAAATCATGGCTTGCGTAAAACACTTTGCATTGTATGGAGCCGGAGAAGCCGGACGCGACTATAATACAGTAGATATGAGCCGCAACCGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAGGCCGGAGTGGGTAGTGTGATGGCGTCATTCAATGAAATAGACGGTATTCCCGCCACCGGAAACAAATGGCTGCTGAGCGATTTGCTGCGTGGCCAGTGGGGCTTCGAAGGGTTTGTGGTAACGGACTTCACAGGCATTTCAGAGATGATAGAGCATGGTGTCGGCGACTTGCAAACCGTCAGTGCACTCGCTCTTAATGCAGGGGTGGACATGGATATGGTAAGTGAGGGCTTCGTCGGTACACTGATGAAATCAATTAAAGAAGGAAAAGTAAGAATGGGCACGTTGAATACAGCCTGCCGCCGGATATTGGAAGCGAAATACAAGCTGGGACTGTTTGACAATCCTTATAAATACTGCGACGTGAACCGTCCGAAGCGGGATATCTTCACAAAAGAGCATCGTGACGCCGCCCGCAAGATTGCCGGCGAAAGTTTTGTTCTTCTGAAGAATGCCCCCGCCACCGCACAGCCACTCGCAGCTCATAGCTCGTCACCCGTAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAAGGTACAGTTGCCGTCATCGGCCCTCTCGGAAATACCCGCAGCAACATGCCGGGCACCTGGAGCGTAGCCGCACGCCTCAACGATTATCCTTCTTTATACGAAGGCTTGAAAGAAATGATGGCAGGCAAGGTGAACATCACCTATGCCAAAGGTAGTAACCTCATCGGCGATGCAGCTTACGAAGAACGTGCCACCATGTTCGGCCGTTCATTGAACCGCGATAATCGCACGGACCAGGAGTTACTGGACGAAGCACTGAAAATTGCAGCCGGCGCCGATGTTATCGTAGCTGCCCTGGGAGAATCTTCTGAAATGAGCGGTGAAAGTTCAAGCCGCACCGAACTCGGCTTGCCCGATGTACAACATACCCTGTTGGAAGCCTTACTGAAAACGGGTAAACCCGTAGTACTAACCCTCTTTACCGGTCGGCCGTTGACGCTGAACTGGGAACAGGAGCATGTACCTGCCATCTTGAATGTATGGTTCGGAGGCAGTGAAGCGGCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTCAATCCGAGTGGAAAACTAACCATGACTTTCCCGAAGAATGTAGGCCAGATACCTTTGTTCTACAATCATAAGAATACCGGTCGGCCACTGGCGGCAGGCAAATGGTTCGAAAAGTTCCGTTCAAACTATCTGGATGTGGATAACGAACCGCTGTATCCCTTCGGTTATGGATTGTCGTATACCACTTTCCAGTACAGTGACATTGCATTGAGCACACCGACATTGGGAAAAGATGGTTCCGTTACAGCCGTAGTCACCGTCACCAATACTGGTAAACATGACGGTGCGGAAGTAGTTCAACTCTATATCCGCGACCTCGTAGGAAGTATCACCCGCCCTGTACGCGAGTTGAAAGGTTTCAATAAAATCTTCCTTCGCGCCGGAGAAAGCAAAACGGTATCATTCACTATCACGCGTGATCTGCTTCGCTTCTATGATTACGACCTGAACTACGTAGCCGAACCGGGTGACTTTGACATCATGATCGGTGGAAACAGCCAGGCTGTGAAGACGGCGAAGTTGACACTT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,724 total letters Query= APC115038.118 Length=2679 Score E Sequences producing significant alignments: (Bits) Value wt 4093 0.0 07H_H1_APC115038.118_H01.seq 830 0.0 K S P Q D M D R F I D A L M K K M T V E E K I G Q L N L P V Query_1 202 AAGTCACCGCAAGACATGGATCGCTTCATCGACGCACTGATGAAGAAGATGACCGTGGAAGAGAAAATCGGACAATTGAACCTACCCGTC 291 2 1 .......................................................................................... 90 T G D I T T G Q A K S S D V A Q K I E K G L V G G L F N L K Query_1 292 ACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCACAAAAGATTGAAAAAGGATTGGTGGGCGGACTCTTCAACCTAAAA 381 2 91 .......................................................................................... 180 G V D R I L E V Q K L A V E K S R L G I P L L F G M Query_1 382 GGTGTAGACCGTATTCTTGAAGTGCAAAAGC-TGG-CAGTAG-AGAAAT-CA-CGC-CTCGG--TATTCCCCT-GCTGTT--CGGCATGG 460 2 181 ...............................-...-......-......-..-...-.....--.........-......--........ 259 1 767 ......N...N......G......T..N...G.....GTN....N...N.....CTC........ 703 D V I H G Y E T I F P I P L G L S C T W D M A A I E K Query_1 461 -ATGTGATA-CAT-GGCTACGAA--ACCATCTT--CCCCATTCCATTGGGATTGTCCTGCACCTGG-GATATGGCGGCTA-TCGAGAAAT 541 2 260 -........-...-.........--........--...............................-.............-......... 340 1 702 N.AT....NN...N....NN...NN...N....ACN..NT....N...N..............N..N.....N.......A....N.NN. 613 S A R I A A I E A S A D G I S W T F S P M V D I S R D Query_1 542 -CCGC-CCGTATTGCAG-CCATCGAAG-CAAGTG-CCGATGGCATTTCC-TGGACATTCAGTCC-GATGGTA-GACAT-CAGTCGCGACC 622 2 341 -....-...........-.........-......-..............-..............-.......-.....-........... 421 1 612 T....T......N..GNT........TA.N....N..............C.............NTN....GNT.....T........... 523 P R W G R V S E G S G E D P F L G G A I A Q A M V Y G Y Q Query_1 623 CACGTTGGGGACGTGTCAG-CGAGGG-CTCGGGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTATACGGATACCAGGG 710 2 422 ...................-......-............................................................... 509 1 522 .N.N..........N....A.NC...A..........................NG.....N....N.T...........N.......... 433 G A N L Q D Q L H R N D E I M A C V K H F A L Y G A G E A G Query_1 711 TGCCAATCTGCAAGACCAATTGCACCGCAACGATGAAATCATGGCTTGCGTAAAACACTTTGCATTGTATGGAGCCGGAGAAGCCGGACG 800 2 510 .......................................................................................... 599 1 432 .......N.....................N..N............................N.......................N.N.. 343 R D Y N T V D M S R N R M F N E F M Y P Y E A A V E A G V G Query_1 801 CGACTATAATACAGTAGATATGAGCCGCAACCGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAGGCCGGAGTGGGTAG 890 2 600 .......................................................................................... 689 1 342 ...N.................................................G.............N...................... 253 S V M A S F N E I D G I P A T G N K W L L S D L L R G Q W G Query_1 891 TGTGATGGCGTCATTCAATGAAATAGACGGTATTCCCGCCACCGGAAACAAATGGCTGCTGAGCGATTTGCTGCGTGGCCAGTGGGGCTT 980 2 690 .......................................................................................... 779 1 252 .......................................................................................... 163 F E G F V V T D W T G I S E M I E H G V G D L Q T V S A Query_1 981 CGAAGGGTTTGTGGTAACGGACTGGACAGGCATTTCAGAGATGATAGAGC-ATGGTGTCGGCGA-CTTGCAAACCGT-CA-GT-GCA-CT 1064 2 780 .......................TC.........................-.............-............-..-..-...-.. 863 1 162 ..................................................A.............T............N..T..G...N.. 73 L A L N A G V D M D M V S E G F V G T L M K S I K E G K Query_1 1065 CGCTCTT---AATGCAGGGGTGGA-CATGGATATGGTAAGTGA-GGGCTTCGT-CGGTACACTGATGAAATCAATTAAAGAAGGAAAAGT 1148 2 864 .......---..............-..................-.........-.................................... 947 1 72 .N...C.TAA..ACNG........C..................G.........N..... 14 V R M G T L N T A C R R I L E A K Y K L G L F D N P Y K Y C Query_1 1149 AAGAATGGGCACGTTGAATACAGCCTGCCGCCGGATATTGGAAGCGAAATACAAGCTGGGACTGTTTGACAATCCTTATAAATACTGCGA 1238 2 948 .......................................................................................... 1037 D V N R P K R D I F T K E H R D A A R K I A G E S F V L L K Query_1 1239 CGTGAACCGTCCGAAGCGGGATATCTTCACAAAAGAGCATCGTGACGCCGCCCGCAAGATTGCCGGCGAAAGTTTTGTTCTTCTGAAGAA 1328 2 1038 .......................................................................................... 1127 N A P A T A Q P L A A H S S S P V T A S P V L P L K K Q G T Query_1 1329 TGCCCCCGCCACCGCACAGCCACTCGCAGCTCATAGCTCGTCACCCGTAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAAGGTACAGT 1418 2 1128 .......................................................................................... 1217 V A V I G P L G N T R S N M P G T W S V A A R L N D Y P S L Query_1 1419 TGCCGTCATCGGCCCTCTCGGAAATACCCGCAGCAACATGCCGGGCACCTGGAGCGTAGCCGCACGCCTCAACGATTATCCTTCTTTATA 1508 2 1218 .......................................................................................... 1307 Y E G L K E M M A G K V N I T Y A K G S N L I G D A A Y E E Query_1 1509 CGAAGGCTTGAAAGAAATGATGGCAGGCAAGGTGAACATCACCTATGCCAAAGGTAGTAACCTCATCGGCGATGCAGCTTACGAAGAACG 1598 2 1308 .......................................................................................... 1397 R A T M F G R S L N R D N R T D Q E L L D E A L K I A A G A Query_1 1599 TGCCACCATGTTCGGCCGTTCATTGAACCGCGATAATCGCACGGACCAGGAGTTACTGGACGAAGCACTGAAAATTGCAGCCGGCGCCGA 1688 2 1398 .......................................................................................... 1487 D V I V A A L G E S S E M S G E S S S R T E L G L P D V Q H Query_1 1689 TGTTATCGTAGCTGCCCTGGGAGAATCTTCTGAAATGAGCGGTGAAAGTTCAAGCCGCACCGAACTCGGCTTGCCCGATGTACAACATAC 1778 2 1488 .......................................................................................... 1577 T L L E A L L K T G K P V V L T L F T G R P L T L N W E Q E Query_1 1779 CCTGTTGGAAGCCTTACTGAAAACGGGTAAACCCGTAGTACTAACCCTCTTTACCGGTCGGCCGTTGACGCTGAACTGGGAACAGGAGCA 1868 2 1578 .......................................................................................... 1667 2 1821 ............ 1832 H V P A I L N V W F G G S E A A Y A I G D V L F G D V N P S Query_1 1869 TGTACCTGCCATCTTGAATGTATGGTTCGGAGGCAGTGAAGCGGCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTCAATCCGAGTGG 1958 2 1668 .......................................................................................... 1757 G K L T M T F P K N V G Q I P L F Y N H K N T G R P L A A G Query_1 1959 AAAACTAACCATGACTTTCCCGAAGAATGTAGGCCAGATACCTTTGTTCTACAATCATAAGAATACCGGTCGGCCACTGGCGGCAGGCAA 2048 2 1758 .......................................................................................... 1847 2 1629 ............ 1640 K W F E K F R S N Y L D V D N E P L Y P F G Y G L S Y T T F Query_1 2049 ATGGTTCGAAAAGTTCCGTTCAAACTATCTGGATGTGGATAACGAACCGCTGTATCCCTTCGGTTATGGATTGTCGTATACCACTTTCCA 2138 2 1848 .......................................................................................... 1937 Q Y S D I A L S T P T L G K D G S V T A V V T V T N T G K H Query_1 2139 GTACAGTGACATTGCATTGAGCACACCGACATTGGGAAAAGATGGTTCCGTTACAGCCGTAGTCACCGTCACCAATACTGGTAAACATGA 2228 2 1938 .......................................................................................... 2027 D G A E V V Q L Y I R D L V G S I T R P V R E L K G F N K I Query_1 2229 CGGTGCGGAAGTAGTTCAACTCTATATCCGCGACCTCGTAGGAAGTATCACCCGCCCTGTACGCGAGTTGAAAGGTTTCAATAAAATCTT 2318 2 2028 .......................................................................................... 2117 F L R A G E S K T V S F T I T R D L L R F Y D Y D L N Y V A Query_1 2319 CCTTCGCGCCGGAGAAAGCAAAACGGTATCATTCACTATCACGCGTGATCTGCTTCGCTTCTATGATTACGACCTGAACTACGTAGCCGA 2408 2 2118 .......................................................................................... 2207 2 2166 ............ 2155 E P G D F D I M I G G N S Q A V K T A K L T L Query_1 2409 ACCGGGTGACTTTGACATCATGATCGGTGGAAACAGCCAGGCTGTGAAGACGGCGAAGTTGACACTT 2475 2 2208 ................................................................... 2274 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 9777526 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:06 PM Number of letters in database: 3,724 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115043.111 (APC115043.26-768.P(769).W283Y.pMCSG68)
KSGDASMNKFIDKLMDRMTLEEKIGQLNLPSSGDITTGQARSNNIADKIRAGAVGGLFNIKGVEKIQEVQRIAVEESRLKIPLLFGMDVIHGYETVFPIPLGMAATWDMKAIEQSARIAAIEASADGICWTFSPMVDISRDPRWGRVSEGSGEDPFLGGEIAKAMVYGYQGKGDSAYREKTNIMACVKHYALYGAAEAGLDYNTTDMSRIRMFNEYMYPYQAAVDAGAGSVMSSFNEVDGIPATANKWLITDVLRKQWGFGGFVVTDYTGIMEMVNHGIGDMREVSARALSAGVDMDMVSEGYLSTLQQSLKEGKITEKEIDQACRRILEAKYKLGLFDNPYKYCDTERAKTDIYTDEHRSIARRISAESFVLLKNDKQTLPIKKKGKIAVVGPLANTSSNMPGTWSVAVNMEAPATLVEGLKEVAGDKVEIVYAKGSHLMSDAAYEERATLFGRTLYRDKEKRSDIQMLNEALNVAHGADVVVAALGESSEMSGESSSRTDLNIPDVQKTLLEELVKTGKPVVLVLFTGRPLTLTWEDKNVSAILNVWFGGTEAAYAIGDVLFGNVNPGGKLPVTFPQNVGQIPLFYNHKNTGRPLAEGGWFEKFRANYLDVTNEPLYPFGYGLSYAQFDYSDVRLSTDQIDRNGMLTASVTVTNNSECDGDEIVQLYIRDLVGSVTRPVKELKGFEKVTIRAGESKDISFKITPEMLKFYNSDIQFVNEVGEFEVMIGTNSRDVKKATFSL
AAGAGTGGGGATGCGTCGATGAACAAATTTATTGATAAACTGATGGACAGGATGACCTTGGAAGAGAAGATTGGTCAGCTTAATCTTCCCAGCTCGGGAGATATAACCACCGGACAGGCACGCAGCAACAATATTGCAGACAAAATCAGAGCAGGTGCAGTGGGTGGCTTATTCAATATAAAAGGAGTTGAGAAGATACAGGAAGTACAACGTATTGCTGTAGAGGAGAGTCGCCTGAAAATTCCTTTACTCTTTGGCATGGATGTTATTCATGGGTATGAAACTGTTTTCCCTATTCCTTTGGGTATGGCTGCCACATGGGATATGAAGGCTATAGAACAATCTGCTCGTATAGCGGCGATAGAAGCCAGTGCCGATGGCATCTGCTGGACATTTAGTCCGATGGTTGATATCAGCCGTGATCCACGTTGGGGACGTGTATCCGAAGGTAGCGGAGAAGATCCTTTTTTAGGTGGTGAAATTGCTAAGGCGATGGTATATGGCTATCAGGGTAAAGGTGATAGCGCATATCGTGAAAAGACTAATATTATGGCTTGTGTGAAGCACTATGCCTTGTATGGGGCAGCAGAAGCCGGTTTGGACTATAATACAACTGACATGAGCCGTATTCGTATGTTTAATGAATATATGTATCCTTATCAGGCGGCTGTGGATGCGGGTGCCGGCAGTGTCATGTCTTCTTTCAACGAGGTCGATGGAATTCCTGCAACAGCCAACAAATGGTTGATAACTGATGTCCTGCGTAAACAGTGGGGATTCGGTGGTTTTGTCGTTACGGACTATACCGGTATCATGGAAATGGTAAATCATGGTATTGGAGATATGCGAGAAGTCTCTGCCCGTGCTTTGAGTGCAGGAGTGGATATGGATATGGTGAGCGAAGGTTATCTTTCTACACTTCAACAATCATTGAAGGAGGGTAAGATAACAGAGAAAGAGATAGATCAAGCTTGCCGTCGTATTTTGGAGGCAAAATATAAGCTGGGATTATTTGATAATCCTTATAAGTATTGTGATACTGAACGTGCCAAAACGGATATCTACACTGATGAACATCGGAGTATTGCACGCCGGATCTCTGCTGAAAGCTTTGTTCTTTTAAAGAATGATAAACAGACACTGCCTATAAAGAAAAAAGGTAAGATTGCTGTAGTTGGGCCGTTGGCGAATACGAGTTCTAATATGCCCGGAACGTGGAGTGTAGCGGTCAATATGGAAGCTCCAGCTACGCTTGTGGAGGGTTTGAAAGAAGTGGCAGGTGATAAAGTTGAAATTGTGTATGCTAAGGGTAGCCATCTGATGAGTGATGCGGCTTATGAGGAACGTGCAACACTCTTTGGACGTACATTATACCGGGATAAGGAAAAACGTTCCGATATCCAGATGCTGAATGAAGCATTAAATGTTGCTCATGGTGCCGATGTTGTTGTTGCGGCATTAGGTGAATCTTCTGAAATGAGTGGTGAATCGAGTAGTCGAACAGATTTGAATATTCCTGATGTTCAAAAAACATTATTGGAAGAATTAGTGAAAACAGGTAAACCTGTCGTTCTGGTATTATTCACTGGGCGTCCGTTGACCCTGACATGGGAAGACAAAAATGTATCTGCTATTCTGAATGTTTGGTTTGGAGGTACCGAAGCCGCTTATGCTATAGGAGATGTCCTATTCGGAAATGTAAATCCTGGAGGTAAGCTGCCTGTAACATTTCCTCAGAATGTAGGGCAGATTCCTTTATTCTATAACCATAAAAATACTGGACGTCCGCTGGCTGAGGGCGGTTGGTTTGAGAAGTTCCGGGCAAATTATCTGGATGTAACGAATGAACCTCTTTATCCATTTGGCTATGGACTAAGTTATGCACAATTTGATTATAGCGATGTGAGATTAAGTACGGATCAAATAGACCGGAATGGCATGTTAACCGCAAGTGTGACTGTAACCAATAACAGTGAGTGTGATGGAGATGAAATTGTTCAGTTGTATATTCGCGATTTGGTCGGTAGTGTTACTCGTCCGGTGAAAGAATTGAAAGGATTTGAAAAAGTAACAATTAGAGCAGGGGAGTCAAAAGATATTTCTTTTAAGATCACTCCGGAAATGCTTAAGTTCTACAATTCGGATATCCAGTTTGTGAATGAAGTTGGTGAATTCGAAGTAATGATCGGAACGAACAGCAGGGATGTGAAAAAAGCAACGTTTAGCTTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,239 total letters Query= APC115043.111 Length=2634 Score E Sequences producing significant alignments: (Bits) Value wt 4011 0.0 K S G D A S M N K F I D K L M D R M T L E E K I G Q L N L P Query_1 202 AAGAGTGGGGATGCGTCGATGAACAAATTTATTGATAAACTGATGGACAGGATGACCTTGGAAGAGAAGATTGGTCAGCTTAATCTTCCC 291 2 1 .......................................................................................... 90 S S G D I T T G Q A R S N N I A D K I R A G A V G G L F N I Query_1 292 AGCTCGGGAGATATAACCACCGGACAGGCACGCAGCAACAATATTGCAGACAAAATCAGAGCAGGTGCAGTGGGTGGCTTATTCAATATA 381 2 91 .......................................................................................... 180 K G V E K I Q E V Q R I A V E E S R L K I P L L F G M D V I Query_1 382 AAAGGAGTTGAGAAGATACAGGAAGTACAACGTATTGCTGTAGAGGAGAGTCGCCTGAAAATTCCTTTACTCTTTGGCATGGATGTTATT 471 2 181 .......................................................................................... 270 H G Y E T V F P I P L G M A A T W D M K A I E Q S A R I A A Query_1 472 CATGGGTATGAAACTGTTTTCCCTATTCCTTTGGGTATGGCTGCCACATGGGATATGAAGGCTATAGAACAATCTGCTCGTATAGCGGCG 561 2 271 .......................................................................................... 360 I E A S A D G I C W T F S P M V D I S R D P R W G R V S E G Query_1 562 ATAGAAGCCAGTGCCGATGGCATCTGCTGGACATTTAGTCCGATGGTTGATATCAGCCGTGATCCACGTTGGGGACGTGTATCCGAAGGT 651 2 361 .......................................................................................... 450 S G E D P F L G G E I A K A M V Y G Y Q G K G D S A Y R E K Query_1 652 AGCGGAGAAGATCCTTTTTTAGGTGGTGAAATTGCTAAGGCGATGGTATATGGCTATCAGGGTAAAGGTGATAGCGCATATCGTGAAAAG 741 2 451 .......................................................................................... 540 T N I M A C V K H Y A L Y G A A E A G L D Y N T T D M S R I Query_1 742 ACTAATATTATGGCTTGTGTGAAGCACTATGCCTTGTATGGGGCAGCAGAAGCCGGTTTGGACTATAATACAACTGACATGAGCCGTATT 831 2 541 .......................................................................................... 630 R M F N E Y M Y P Y Q A A V D A G A G S V M S S F N E V D G Query_1 832 CGTATGTTTAATGAATATATGTATCCTTATCAGGCGGCTGTGGATGCGGGTGCCGGCAGTGTCATGTCTTCTTTCAACGAGGTCGATGGA 921 2 631 .......................................................................................... 720 I P A T A N K W L I T D V L R K Q Y G F G G F V V T D Y T G Query_1 922 ATTCCTGCAACAGCCAACAAATGGTTGATAACTGATGTCCTGCGTAAACAGTATGGATTCGGTGGTTTTGTCGTTACGGACTATACCGGT 1011 2 721 ....................................................GG.................................... 810 I M E M V N H G I G D M R E V S A R A L S A G V D M D M V S Query_1 1012 ATCATGGAAATGGTAAATCATGGTATTGGAGATATGCGAGAAGTCTCTGCCCGTGCTTTGAGTGCAGGAGTGGATATGGATATGGTGAGC 1101 2 811 .......................................................................................... 900 E G Y L S T L Q Q S L K E G K I T E K E I D Q A C R R I L E Query_1 1102 GAAGGTTATCTTTCTACACTTCAACAATCATTGAAGGAGGGTAAGATAACAGAGAAAGAGATAGATCAAGCTTGCCGTCGTATTTTGGAG 1191 2 901 .......................................................................................... 990 A K Y K L G L F D N P Y K Y C D T E R A K T D I Y T D E H R Query_1 1192 GCAAAATATAAGCTGGGATTATTTGATAATCCTTATAAGTATTGTGATACTGAACGTGCCAAAACGGATATCTACACTGATGAACATCGG 1281 2 991 .......................................................................................... 1080 S I A R R I S A E S F V L L K N D K Q T L P I K K K G K I A Query_1 1282 AGTATTGCACGCCGGATCTCTGCTGAAAGCTTTGTTCTTTTAAAGAATGATAAACAGACACTGCCTATAAAGAAAAAAGGTAAGATTGCT 1371 2 1081 .......................................................................................... 1170 V V G P L A N T S S N M P G T W S V A V N M E A P A T L V E Query_1 1372 GTAGTTGGGCCGTTGGCGAATACGAGTTCTAATATGCCCGGAACGTGGAGTGTAGCGGTCAATATGGAAGCTCCAGCTACGCTTGTGGAG 1461 2 1171 .......................................................................................... 1260 G L K E V A G D K V E I V Y A K G S H L M S D A A Y E E R A Query_1 1462 GGTTTGAAAGAAGTGGCAGGTGATAAAGTTGAAATTGTGTATGCTAAGGGTAGCCATCTGATGAGTGATGCGGCTTATGAGGAACGTGCA 1551 2 1261 .......................................................................................... 1350 T L F G R T L Y R D K E K R S D I Q M L N E A L N V A H G A Query_1 1552 ACACTCTTTGGACGTACATTATACCGGGATAAGGAAAAACGTTCCGATATCCAGATGCTGAATGAAGCATTAAATGTTGCTCATGGTGCC 1641 2 1351 .......................................................................................... 1440 D V V V A A L G E S S E M S G E S S S R T D L N I P D V Q K Query_1 1642 GATGTTGTTGTTGCGGCATTAGGTGAATCTTCTGAAATGAGTGGTGAATCGAGTAGTCGAACAGATTTGAATATTCCTGATGTTCAAAAA 1731 2 1441 .......................................................................................... 1530 T L L E E L V K T G K P V V L V L F T G R P L T L T W E D K Query_1 1732 ACATTATTGGAAGAATTAGTGAAAACAGGTAAACCTGTCGTTCTGGTATTATTCACTGGGCGTCCGTTGACCCTGACATGGGAAGACAAA 1821 2 1531 .......................................................................................... 1620 N V S A I L N V W F G G T E A A Y A I G D V L F G N V N P G Query_1 1822 AATGTATCTGCTATTCTGAATGTTTGGTTTGGAGGTACCGAAGCCGCTTATGCTATAGGAGATGTCCTATTCGGAAATGTAAATCCTGGA 1911 2 1621 .......................................................................................... 1710 G K L P V T F P Q N V G Q I P L F Y N H K N T G R P L A E G Query_1 1912 GGTAAGCTGCCTGTAACATTTCCTCAGAATGTAGGGCAGATTCCTTTATTCTATAACCATAAAAATACTGGACGTCCGCTGGCTGAGGGC 2001 2 1711 .......................................................................................... 1800 G W F E K F R A N Y L D V T N E P L Y P F G Y G L S Y A Q F Query_1 2002 GGTTGGTTTGAGAAGTTCCGGGCAAATTATCTGGATGTAACGAATGAACCTCTTTATCCATTTGGCTATGGACTAAGTTATGCACAATTT 2091 2 1801 .......................................................................................... 1890 D Y S D V R L S T D Q I D R N G M L T A S V T V T N N S E C Query_1 2092 GATTATAGCGATGTGAGATTAAGTACGGATCAAATAGACCGGAATGGCATGTTAACCGCAAGTGTGACTGTAACCAATAACAGTGAGTGT 2181 2 1891 .......................................................................................... 1980 D G D E I V Q L Y I R D L V G S V T R P V K E L K G F E K V Query_1 2182 GATGGAGATGAAATTGTTCAGTTGTATATTCGCGATTTGGTCGGTAGTGTTACTCGTCCGGTGAAAGAATTGAAAGGATTTGAAAAAGTA 2271 2 1981 .......................................................................................... 2070 T I R A G E S K D I S F K I T P E M L K F Y N S D I Q F V N Query_1 2272 ACAATTAGAGCAGGGGAGTCAAAAGATATTTCTTTTAAGATCACTCCGGAAATGCTTAAGTTCTACAATTCGGATATCCAGTTTGTGAAT 2361 2 2071 .......................................................................................... 2160 E V G E F E V M I G T N S R D V K K A T F S L Query_1 2362 GAAGTTGGTGAATTCGAAGTAATGATCGGAACGAACAGCAGGGATGTGAAAAAAGCAACGTTTAGCTTG 2430 2 2161 ..................................................................... 2229 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5736038 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:06 PM Number of letters in database: 2,239 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115044.107 (APC115044.33-744.P(748).D280A.pMCSG68)
VESLLSKMTLEEKIGQMNQISSYGNIEDMSALIKKGEIGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQVAKDGARIAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKNVLRDEWGFDGFVVTDWASASEMISHGFAAGSKEVAMKSVNAGVDMEMVSYTFVKELPELVKEGKVKESTIDEAVRNILRIKYRLGLFDTPYVDEQQTSVMYAPSHLEAAKQAAVESAILLKNDKEVLPLQPSVKTVAVVGPMANAPYEQLGTWIFDGEKARTQTPLNAIKEMVGDKVQVIYEPGLAYSREKNPASVAKAAAAAARADVILAFVGEESILSGEAHCLADLDLQGDQGALITALAKTGKPVVTIVMAGRPLTIGKEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKAPVTFPRMVGQIPVYYAHNNTGRPATRNEVLLNDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNIKLASDVLKKDDVLTVTFDLENTGKYEGTEVAQLYIQDKIGSVTRPVKELKRFTRVTLKPGEKKSVSFELPVSELAFWNIDMAKVVEPGDFGLWVATDSQSGEEVFF
GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGTGCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAATCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCGTTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATTGATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGCAGCTATGGTAGAAGGTTTTCAGGGAGATTCGCTGAATAGTCCTACTTCAATTGCAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAGCCGAAGGAGGACGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAGCGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAATGTACTCCGTGACGAGTGGGGATTCGATGGTTTTGTTGTGACGGACTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGTTCAAAAGAAGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTGAAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCTTATGTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTGAAGAATGATAAGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTAGGTACTTGGATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATTTATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTGAATGATATTGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTTGTCGTACACCACATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATTCGATCTGGAAAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGAAAAAAAGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCTTC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,966 total letters Query= APC115044.107 Length=2541 Score E Sequences producing significant alignments: (Bits) Value wt 3844 0.0 08B_B2_APC115044.107_B02.seq 1209 0.0 02B_B2_APC115044.107_B02.seq 275 1e-76 V E S L L S K M T L E E K I G Q M N Q I S S Y G N Query_1 202 GTAGAATCTCTCCTGTCTAAGATG-ACCCT--TGAGGAGAAAA-TCGG-TCAG-ATGAA-CCA-GATTT-CCT---CTTACGG-TAATAT 278 2 1 ........................-.....--...........-....-....-.....-...-.....-...---.......-...... 77 1 840 ..N..N.....N.N.....GN.AG..N.....A.N..A....N.....A...N.....T...TCAN.AN...N...... 762 I E D M S A L I K K G E I G S I L N E V D P V R I N A Query_1 279 C-GAG-GATA-TGAGTG-CT-TTGATTAAGAAAGGTGAAA-TCGGTT--CCATCTTGAATG-AGGTGGA-TC-CGGTGCGTATTAATGCG 357 2 78 .-...-....-......-..-...................-......--............-.......-..-................. 156 1 761 TA...A....A......N..G..N....N....A.N....A....N.NC............N....N..A..G................. 672 L Q R V A M E E S R L G I P L L I A R D V I H G F K T I F P Query_1 358 CTACAGCGCGTGGCAATGGAAGAATCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCT 447 2 157 .......................................................................................... 246 1 671 N..............................N...................N........................N............. 582 I P L G Q A A S F N P Q V A K D G A R I A A I E A S S V G Query_1 448 ATTCCCTTGGGACAAGCGGCTTCGTTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAG-CAG-CTATTGAAGCTTCGTCTGTAGGTA 535 2 247 .............................................................-...-........................ 334 1 581 .............................................................N...G........................ 492 I R W T F A P M I D I A R D P R W G R I A E G C G E D T Y L Query_1 536 TCCGGTGGACTTTTGCGCCAATGATTGATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTA 625 2 335 .......................................................................................... 424 1 491 ................A......................................................................... 402 T S V M G A A M V E G F Q G D S L N S P T S I A A C P K H F Query_1 626 CTTCCGTAATGGGAGCAGCTATGGTAGAAGGTTTTCAGGGAGATTCGCTGAATAGTCCTACTTCAATTGCAGCTTGCCCTAAACATTTTG 715 2 425 .......................................................................................... 514 1 401 ................................................N......................................... 312 V G Y G A A E G G R D Y N S T F I P E R R L R N V Y L P P F Query_1 716 TAGGTTACGGTGCAGCCGAAGGAGGACGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTG 805 2 515 .......................................................................................... 604 1 311 .......................................................................................... 222 E A A T K A G A A T F M T S F N D N D G I P S T G N A F I L Query_1 806 AAGCTGCCACCAAAGCGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGA 895 2 605 .......................................................................................... 694 1 221 .......................................................................................... 132 K N V L R D E W G F D G F V V T A W A S A S E M I S H G F A Query_1 896 AGAATGTACTCCGTGACGAGTGGGGATTCGATGGTTTTGTTGTGACGGCTTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCG 985 2 695 ................................................AC........................................ 784 1 131 .......................................................................................... 42 0 46 ...N...N...CN.NN..........N..............N-...............N....N 108 A G S K E V A M K S V N A G V D M E M V S Y T F V K E L P E Query_1 986 CCGGTTCAAAAGAAGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAAT 1075 2 785 .......................................................................................... 874 1 41 ........................... 15 0 109 ..C........-.T.N..N......-.......NN.NNN.......N-..N....N...N.....N.........NN..N....NNN.N. 195 L V K E G K V K E S T I D E A V R N I L R I K Y R L G L F D Query_1 1076 TAGTGAAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATA 1165 2 875 .......................................................................................... 964 0 196 .TT.N...N...N...N.........T..C..........T.....C.N........CN..................NN........... 285 T P Y V D E Q Q T S V M Y A P S H L E A A K Q A A V E S A I Query_1 1166 CACCTTATGTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTC 1255 2 965 .......................................................................................... 1054 0 286 TN....N...TNN.N.N.N.TN...NC....T.NN.N.T...N.....N...NN..N..N..T.TNNC..NN.N...N....NN..N... 375 L L K N D K E V L P L Q P S V K T V A V V G P M A N A P Y E Query_1 1256 TGTTGAAGAATGATAAGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAAC 1345 2 1055 .......................................................................................... 1144 0 376 ......NN...... 389 Q L G T W I F D G E K A R T Q T P L N A I K E M V G D K V Q Query_1 1346 AGTTAGGTACTTGGATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGG 1435 2 1145 .......................................................................................... 1234 V I Y E P G L A Y S R E K N P A S V A K A A A A A A R A D V Query_1 1436 TGATTTATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCA 1525 2 1235 .......................................................................................... 1324 2 1318 ............ 1307 I L A F V G E E S I L S G E A H C L A D L D L Q G D Q G A L Query_1 1526 TTCTTGCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGA 1615 2 1325 .......................................................................................... 1414 I T A L A K T G K P V V T I V M A G R P L T I G K E V E E S Query_1 1616 TTACAGCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGA 1705 2 1415 .......................................................................................... 1504 T A V L Y S F H P G T M G G P A L A D L L W G K A V P S G K Query_1 1706 CTGCTGTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGG 1795 2 1505 .......................................................................................... 1594 A P V T F P R M V G Q I P V Y Y A H N N T G R P A T R N E V Query_1 1796 CGCCGGTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGT 1885 2 1595 .......................................................................................... 1684 2 1666 ............ 1655 L L N D I A V E A G Q T S L G C T S F Y M D A G F D P L F P Query_1 1886 TGCTGAATGATATTGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGT 1975 2 1685 .......................................................................................... 1774 F G Y G L S Y T T F K Y S N I K L A S D V L K K D D V L T V Query_1 1976 TTGGTTATGGCTTGTCGTACACCACATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGA 2065 2 1775 .......................................................................................... 1864 T F D L E N T G K Y E G T E V A Q L Y I Q D K I G S V T R P Query_1 2066 CATTCGATCTGGAAAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGG 2155 2 1865 .......................................................................................... 1954 2 1926 ............ 1915 V K E L K R F T R V T L K P G E K K S V S F E L P V S E L A Query_1 2156 TGAAAGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGaaaaaaaGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCAT 2245 2 1955 .......................................................................................... 2044 F W N I D M A K V V E P G D F G L W V A T D S Q S G E E V F Query_1 2246 TTTGGAACATAGATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCT 2335 2 2045 .......................................................................................... 2134 F Query_1 2336 TC 2337 2 2135 .. 2136 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 9881460 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:06 PM Number of letters in database: 3,966 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115044.108 (APC115044.33-744.P(748).E487A.pMCSG68)
VESLLSKMTLEEKIGQMNQISSYGNIEDMSALIKKGEIGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQVAKDGARIAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKNVLRDEWGFDGFVVTDWASASEMISHGFAAGSKEVAMKSVNAGVDMEMVSYTFVKELPELVKEGKVKESTIDEAVRNILRIKYRLGLFDTPYVDEQQTSVMYAPSHLEAAKQAAVESAILLKNDKEVLPLQPSVKTVAVVGPMANAPYEQLGTWIFDGEKARTQTPLNAIKEMVGDKVQVIYEPGLAYSREKNPASVAKAAAAAARADVILAFVGEESILSGEAHCLADLDLQGDQGALITALAKTGKPVVTIVMAGRPLTIGKEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKAPVTFPRMVGQIPVYYAHNNTGRPATRNEVLLNDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNIKLASDVLKKDDVLTVTFDLENTGKYEGTEVAQLYIQDKIGSVTRPVKELKRFTRVTLKPGEKKSVSFELPVSELAFWNIDMAKVVEPGDFGLWVATDSQSGEEVFF
GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGTGCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAATCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCGTTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATTGATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGCAGCTATGGTAGAAGGTTTTCAGGGAGATTCGCTGAATAGTCCTACTTCAATTGCAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAGCCGAAGGAGGACGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAGCGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAATGTACTCCGTGACGAGTGGGGATTCGATGGTTTTGTTGTGACGGACTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGTTCAAAAGAAGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTGAAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCTTATGTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTGAAGAATGATAAGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTAGGTACTTGGATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATTTATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTGAATGATATTGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTTGTCGTACACCACATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATTCGATCTGGAAAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGAAAAAAAGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCTTC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,330 total letters Query= APC115044.108 Length=2541 Score E Sequences producing significant alignments: (Bits) Value wt 3844 0.0 02C_C2_APC115044.108_C02.seq 145 9e-38 V E S L L S K M T L E E K I G Q M N Q I S S Y G N I E D M S Query_1 202 GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGT 291 2 1 .......................................................................................... 90 A L I K K G E I G S I L N E V D P V R I N A L Q R V A M E E Query_1 292 GCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAA 381 2 91 .......................................................................................... 180 S R L G I P L L I A R D V I H G F K T I F P I P L G Q A A S Query_1 382 TCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCG 471 2 181 .......................................................................................... 270 F N P Q V A K D G A R I A A I E A S S V G I R W T F A P M I Query_1 472 TTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATT 561 2 271 .......................................................................................... 360 D I A R D P R W G R I A E G C G E D T Y L T S V M G A A M V Query_1 562 GATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGCAGCTATGGTA 651 2 361 .......................................................................................... 450 E G F Q G D S L N S P T S I A A C P K H F V G Y G A A E G G Query_1 652 GAAGGTTTTCAGGGAGATTCGCTGAATAGTCCTACTTCAATTGCAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAGCCGAAGGAGGA 741 2 451 .......................................................................................... 540 R D Y N S T F I P E R R L R N V Y L P P F E A A T K A G A A Query_1 742 CGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAGCGGGTGCAGCC 831 2 541 .......................................................................................... 630 T F M T S F N D N D G I P S T G N A F I L K N V L R D E W G Query_1 832 ACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAATGTACTCCGTGACGAGTGGGGA 921 2 631 .......................................................................................... 720 F D G F V V T D W A S A S E M I S H G F A A G S K E V A M K Query_1 922 TTCGATGGTTTTGTTGTGACGGACTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGTTCAAAAGAAGTGGCAATGAAA 1011 2 721 .......................................................................................... 810 S V N A G V D M E M V S Y T F V K E L P E L V K E G K V K E Query_1 1012 TCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTGAAAGAGGGAAAGGTGAAGGAA 1101 2 811 .......................................................................................... 900 S T I D E A V R N I L R I K Y R L G L F D T P Y V D E Q Q T Query_1 1102 AGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCTTATGTAGATGAACAACAAACA 1191 2 901 .......................................................................................... 990 S V M Y A P S H L E A A K Q A A V E S A I L L K N D K E V L Query_1 1192 TCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTGAAGAATGATAAGGAAGTGTTG 1281 2 991 .......................................................................................... 1080 P L Q P S V K T V A V V G P M A N A P Y E Q L G T W I F D G Query_1 1282 CCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTAGGTACTTGGATATTTGATGGT 1371 2 1081 .......................................................................................... 1170 E K A R T Q T P L N A I K E M V G D K V Q V I Y E P G L A Y Query_1 1372 GAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATTTATGAACCGGGACTAGCATAT 1461 2 1171 .......................................................................................... 1260 S R E K N P A S V A K A A A A A A R A D V I L A F V G E E Query_1 1462 AGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTT-GTGGGTGAAGAATC 1550 2 1261 ...........................................................................-.............. 1349 2 1318 ............ 1307 0 54 ........N........NN.... 76 S I L S G A A H C L A D L D L Q G D Q G A L I T A L A K T G Query_1 1551 TATTCTTTCGGGTGCTGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGCTAAGACGGGTAA 1640 2 1350 ..............AA.......................................................................... 1439 0 77 ........N.NNC..N............N..........-....NC........N.....N.......N.N.N......N.N....N... 165 K P V V T I V M A G R P L T I G K E V E E S T A V L Y S F H Query_1 1641 ACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTATTCATTCCATCC 1730 2 1440 .......................................................................................... 1529 0 166 N....C.......N.N.C...... 189 P G T M G G P A L A D L L W G K A V P S G K A P V T F P R M Query_1 1731 GGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTTCCCGAGGATGGT 1820 2 1530 .......................................................................................... 1619 V G Q I P V Y Y A H N N T G R P A T R N E V L L N D I A V E Query_1 1821 GGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTGAATGATATTGCTGTTGAGGC 1910 2 1620 .......................................................................................... 1709 2 1666 ............ 1655 A G Q T S L G C T S F Y M D A G F D P L F P F G Y G L S Y T Query_1 1911 AGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTTGTCGTACACCAC 2000 2 1710 .......................................................................................... 1799 T F K Y S N I K L A S D V L K K D D V L T V T F D L E N T G Query_1 2001 ATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATTCGATCTGGAAAATACCGGGAA 2090 2 1800 .......................................................................................... 1889 K Y E G T E V A Q L Y I Q D K I G S V T R P V K E L K R F T Query_1 2091 ATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCG 2180 2 1890 .......................................................................................... 1979 2 1926 ............ 1915 R V T L K P G E K K S V S F E L P V S E L A F W N I D M A K Query_1 2181 TGTGACATTGAAGCCGGGTGAGaaaaaaaGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGT 2270 2 1980 .......................................................................................... 2069 V V E P G D F G L W V A T D S Q S G E E V F F Query_1 2271 TGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCTTC 2337 2 2070 ................................................................... 2136 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5762050 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:06 PM Number of letters in database: 2,330 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115044.109 (APC115044.33-744.P(748).W418A.pMCSG68)
VESLLSKMTLEEKIGQMNQISSYGNIEDMSALIKKGEIGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQVAKDGARIAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKNVLRDEWGFDGFVVTDWASASEMISHGFAAGSKEVAMKSVNAGVDMEMVSYTFVKELPELVKEGKVKESTIDEAVRNILRIKYRLGLFDTPYVDEQQTSVMYAPSHLEAAKQAAVESAILLKNDKEVLPLQPSVKTVAVVGPMANAPYEQLGTWIFDGEKARTQTPLNAIKEMVGDKVQVIYEPGLAYSREKNPASVAKAAAAAARADVILAFVGEESILSGEAHCLADLDLQGDQGALITALAKTGKPVVTIVMAGRPLTIGKEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKAPVTFPRMVGQIPVYYAHNNTGRPATRNEVLLNDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNIKLASDVLKKDDVLTVTFDLENTGKYEGTEVAQLYIQDKIGSVTRPVKELKRFTRVTLKPGEKKSVSFELPVSELAFWNIDMAKVVEPGDFGLWVATDSQSGEEVFF
GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGTGCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAATCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCGTTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATTGATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGCAGCTATGGTAGAAGGTTTTCAGGGAGATTCGCTGAATAGTCCTACTTCAATTGCAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAGCCGAAGGAGGACGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAGCGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAATGTACTCCGTGACGAGTGGGGATTCGATGGTTTTGTTGTGACGGACTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGTTCAAAAGAAGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTGAAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCTTATGTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTGAAGAATGATAAGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTAGGTACTTGGATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATTTATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTGAATGATATTGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTTGTCGTACACCACATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATTCGATCTGGAAAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGAAAAAAAGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCTTC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,146 total letters Query= APC115044.109 Length=2541 Score E Sequences producing significant alignments: (Bits) Value wt 3838 0.0 V E S L L S K M T L E E K I G Q M N Q I S S Y G N I E D M S Query_1 202 GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGT 291 2 1 .......................................................................................... 90 A L I K K G E I G S I L N E V D P V R I N A L Q R V A M E E Query_1 292 GCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAA 381 2 91 .......................................................................................... 180 S R L G I P L L I A R D V I H G F K T I F P I P L G Q A A S Query_1 382 TCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCG 471 2 181 .......................................................................................... 270 F N P Q V A K D G A R I A A I E A S S V G I R W T F A P M I Query_1 472 TTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATT 561 2 271 .......................................................................................... 360 D I A R D P R W G R I A E G C G E D T Y L T S V M G A A M V Query_1 562 GATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGCAGCTATGGTA 651 2 361 .......................................................................................... 450 E G F Q G D S L N S P T S I A A C P K H F V G Y G A A E G G Query_1 652 GAAGGTTTTCAGGGAGATTCGCTGAATAGTCCTACTTCAATTGCAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAGCCGAAGGAGGA 741 2 451 .......................................................................................... 540 R D Y N S T F I P E R R L R N V Y L P P F E A A T K A G A A Query_1 742 CGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAGCGGGTGCAGCC 831 2 541 .......................................................................................... 630 T F M T S F N D N D G I P S T G N A F I L K N V L R D E W G Query_1 832 ACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAATGTACTCCGTGACGAGTGGGGA 921 2 631 .......................................................................................... 720 F D G F V V T D W A S A S E M I S H G F A A G S K E V A M K Query_1 922 TTCGATGGTTTTGTTGTGACGGACTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGTTCAAAAGAAGTGGCAATGAAA 1011 2 721 .......................................................................................... 810 S V N A G V D M E M V S Y T F V K E L P E L V K E G K V K E Query_1 1012 TCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTGAAAGAGGGAAAGGTGAAGGAA 1101 2 811 .......................................................................................... 900 S T I D E A V R N I L R I K Y R L G L F D T P Y V D E Q Q T Query_1 1102 AGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCTTATGTAGATGAACAACAAACA 1191 2 901 .......................................................................................... 990 S V M Y A P S H L E A A K Q A A V E S A I L L K N D K E V L Query_1 1192 TCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTGAAGAATGATAAGGAAGTGTTG 1281 2 991 .......................................................................................... 1080 P L Q P S V K T V A V V G P M A N A P Y E Q L G T A I F D G Query_1 1282 CCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTAGGTACTGCTATATTTGATGGT 1371 2 1081 ...........................................................................TGG............ 1170 E K A R T Q T P L N A I K E M V G D K V Q V I Y E P G L A Y Query_1 1372 GAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATTTATGAACCGGGACTAGCATAT 1461 2 1171 .......................................................................................... 1260 S R E K N P A S V A K A A A A A A R A D V I L A F V G E E S Query_1 1462 AGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAATCT 1551 2 1261 .......................................................................................... 1350 2 1318 ............ 1307 I L S G E A H C L A D L D L Q G D Q G A L I T A L A K T G K Query_1 1552 ATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGCTAAGACGGGTAAA 1641 2 1351 .......................................................................................... 1440 P V V T I V M A G R P L T I G K E V E E S T A V L Y S F H P Query_1 1642 CCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTATTCATTCCATCCG 1731 2 1441 .......................................................................................... 1530 G T M G G P A L A D L L W G K A V P S G K A P V T F P R M V Query_1 1732 GGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTTCCCGAGGATGGTG 1821 2 1531 .......................................................................................... 1620 G Q I P V Y Y A H N N T G R P A T R N E V L L N D I A V E A Query_1 1822 GGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTGAATGATATTGCTGTTGAGGCA 1911 2 1621 .......................................................................................... 1710 2 1666 ............ 1655 G Q T S L G C T S F Y M D A G F D P L F P F G Y G L S Y T T Query_1 1912 GGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTTGTCGTACACCACA 2001 2 1711 .......................................................................................... 1800 F K Y S N I K L A S D V L K K D D V L T V T F D L E N T G K Query_1 2002 TTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATTCGATCTGGAAAATACCGGGAAA 2091 2 1801 .......................................................................................... 1890 Y E G T E V A Q L Y I Q D K I G S V T R P V K E L K R F T R Query_1 2092 TATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCGT 2181 2 1891 .......................................................................................... 1980 2 1926 ............ 1915 V T L K P G E K K S V S F E L P V S E L A F W N I D M A K V Query_1 2182 GTGACATTGAAGCCGGGTGAGaaaaaaaGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGTT 2271 2 1981 .......................................................................................... 2070 V E P G D F G L W V A T D S Q S G E E V F F Query_1 2272 GTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCTTC 2337 2 2071 .................................................................. 2136 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5297450 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 2,146 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115044.115 (APC115044.33-744.P(748).F248A.pMCSG68)
VESLLSKMTLEEKIGQMNQISSYGNIEDMSALIKKGEIGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQVAKDGARIAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKNVLRDEWGFDGFVVTDWASASEMISHGFAAGSKEVAMKSVNAGVDMEMVSYTFVKELPELVKEGKVKESTIDEAVRNILRIKYRLGLFDTPYVDEQQTSVMYAPSHLEAAKQAAVESAILLKNDKEVLPLQPSVKTVAVVGPMANAPYEQLGTWIFDGEKARTQTPLNAIKEMVGDKVQVIYEPGLAYSREKNPASVAKAAAAAARADVILAFVGEESILSGEAHCLADLDLQGDQGALITALAKTGKPVVTIVMAGRPLTIGKEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKAPVTFPRMVGQIPVYYAHNNTGRPATRNEVLLNDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNIKLASDVLKKDDVLTVTFDLENTGKYEGTEVAQLYIQDKIGSVTRPVKELKRFTRVTLKPGEKKSVSFELPVSELAFWNIDMAKVVEPGDFGLWVATDSQSGEEVFF
GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGTGCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAATCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCGTTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATTGATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGCAGCTATGGTAGAAGGTTTTCAGGGAGATTCGCTGAATAGTCCTACTTCAATTGCAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAGCCGAAGGAGGACGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAGCGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAATGTACTCCGTGACGAGTGGGGATTCGATGGTTTTGTTGTGACGGACTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGTTCAAAAGAAGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTGAAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCTTATGTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTGAAGAATGATAAGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTAGGTACTTGGATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATTTATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTGAATGATATTGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTTGTCGTACACCACATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATTCGATCTGGAAAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGAAAAAAAGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCTTC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,534 total letters Query= APC115044.115 Length=2541 Score E Sequences producing significant alignments: (Bits) Value wt 3844 0.0 02E_E2_APC115044.115_E02.seq 1115 0.0 V E S L L S K M T L E E K I G Q M N Q I S S Y G N I E D M S Query_1 202 GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGT 291 2 1 .......................................................................................... 90 A L I K K G E I G S I L N E V D P V R I N A L Q R V A M E E Query_1 292 GCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAA 381 2 91 .......................................................................................... 180 S R L G I P L L I A R D V I H G F K T I F P I P L G Q A A S Query_1 382 TCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCG 471 2 181 .......................................................................................... 270 F N P Q V A K D G A R I A A I E A S S V G I R W T F A P M I Query_1 472 TTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATT 561 2 271 .......................................................................................... 360 D I A R D P R W G R I A E G C G E D T Y L T S V M G A A M Query_1 562 GATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGC-AGCTATG-G 649 2 361 ................................................................................-.......-. 448 0 6 ......NN.C......CN......N. 31 V E G F Q G D S L N S P T S I A A C P K H F V G Y G A Query_1 650 TAGAAG-GTTTTCAGGG-AGAT-TCG--CTGAATAGTCC---TACTTCAATTGCAGCTTGCCCTAAACATTTTGT-AGGTTACGG-TGCA 729 2 449 ......-..........-....-...--...........---.................................-.........-.... 528 0 32 ......T..........T....C...GCN.......C..CNT.TN..............................T.........C.... 121 A E G G R D Y N S T F I P E R R L R N V Y L P P F E A A T K Query_1 730 GCCGAAGGAGGACGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAA 819 2 529 .......................................................................................... 618 0 122 .......................................................................................... 211 A G A A T F M T S A N D N D G I P S T G N A F I L K N V L R Query_1 820 GCGGGTGCAGCCACGTTTATGACTTCAGCTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAATGTACTCCGT 909 2 619 ...........................TT............................................................. 708 0 212 .......................................................................................... 301 D E W G F D G F V V T D W A S A S E M I S H G F A A G S K E Query_1 910 GACGAGTGGGGATTCGATGGTTTTGTTGTGACGGACTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGTTCAAAAGAA 999 2 709 .......................................................................................... 798 0 302 .......................................................................................... 391 V A M K S V N A G V D M E M V S Y T F V K E L P E L V K E G Query_1 1000 GTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTGAAAGAGGGA 1089 2 799 .......................................................................................... 888 0 392 ...........................N..................................................N.......N... 481 K V K E S T I D E A V R N I L R I K Y R L G L F D T P Y V Query_1 1090 AAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGT-TTGATACACCTTATGT-AG 1177 2 889 ......................................................................-................-.. 976 0 482 ...................N..................N....................C...N......C.............N.NC.. 571 D E Q Q T S V M Y A P S H L E A A K Q A A V E S A I L L K Query_1 1178 ATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAA-GCAAGCCGCTGTTGAATCGGCTATTCTGTT-GAAGAA 1265 2 977 ....................................................-..............................-...... 1064 0 572 .......................N.............N.........N....NN.............................G...N.. 661 N D K E V L P L Q P S V K T V A V V G P M A N A P Y E Q L G Query_1 1266 TGATAAGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCA-CCTTATGAACAGT-TAGGT 1353 2 1065 ......................................................................-.............-..... 1152 0 662 ...............T.......NN..............N...NN.....N.N...N.....N......NC.....NN...N.NC..N.N 751 T W I F D G E K A R T Q T P L N A I K E M V G D K V Q Query_1 1354 ACTTGGATATTTGATGGTGAGAAAGCT-CGTACTC-AGAC-TCCGT-TGAACGCTA-TTAA-AG-AAAT-GGTTGGCGATAAAGTACAGG 1435 2 1153 ...........................-.......-....-.....-.........-....-..-....-.................... 1234 0 752 .........N.......N..N.....NC.......A....T...N.C...C.T...N....N..T....G....... 828 V I Y E P G L A Y S R E K N P A S V A K A A A A A A R A D V Query_1 1436 TGATTTATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCA 1525 2 1235 .......................................................................................... 1324 2 1318 ............ 1307 I L A F V G E E S I L S G E A H C L A D L D L Q G D Q G A L Query_1 1526 TTCTTGCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGA 1615 2 1325 .......................................................................................... 1414 I T A L A K T G K P V V T I V M A G R P L T I G K E V E E S Query_1 1616 TTACAGCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGA 1705 2 1415 .......................................................................................... 1504 T A V L Y S F H P G T M G G P A L A D L L W G K A V P S G K Query_1 1706 CTGCTGTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGG 1795 2 1505 .......................................................................................... 1594 A P V T F P R M V G Q I P V Y Y A H N N T G R P A T R N E V Query_1 1796 CGCCGGTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGT 1885 2 1595 .......................................................................................... 1684 2 1666 ............ 1655 L L N D I A V E A G Q T S L G C T S F Y M D A G F D P L F P Query_1 1886 TGCTGAATGATATTGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGT 1975 2 1685 .......................................................................................... 1774 F G Y G L S Y T T F K Y S N I K L A S D V L K K D D V L T V Query_1 1976 TTGGTTATGGCTTGTCGTACACCACATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGA 2065 2 1775 .......................................................................................... 1864 T F D L E N T G K Y E G T E V A Q L Y I Q D K I G S V T R P Query_1 2066 CATTCGATCTGGAAAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGG 2155 2 1865 .......................................................................................... 1954 2 1926 ............ 1915 V K E L K R F T R V T L K P G E K K S V S F E L P V S E L A Query_1 2156 TGAAAGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGaaaaaaaGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCAT 2245 2 1955 .......................................................................................... 2044 F W N I D M A K V V E P G D F G L W V A T D S Q S G E E V F Query_1 2246 TTTGGAACATAGATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCT 2335 2 2045 .......................................................................................... 2134 F Query_1 2336 TC 2337 2 2135 .. 2136 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 8791092 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 3,534 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115044.116 (APC115044.33-744.P(748).W281A.pMCSG68)
VESLLSKMTLEEKIGQMNQISSYGNIEDMSALIKKGEIGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQVAKDGARIAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKNVLRDEWGFDGFVVTDWASASEMISHGFAAGSKEVAMKSVNAGVDMEMVSYTFVKELPELVKEGKVKESTIDEAVRNILRIKYRLGLFDTPYVDEQQTSVMYAPSHLEAAKQAAVESAILLKNDKEVLPLQPSVKTVAVVGPMANAPYEQLGTWIFDGEKARTQTPLNAIKEMVGDKVQVIYEPGLAYSREKNPASVAKAAAAAARADVILAFVGEESILSGEAHCLADLDLQGDQGALITALAKTGKPVVTIVMAGRPLTIGKEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKAPVTFPRMVGQIPVYYAHNNTGRPATRNEVLLNDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNIKLASDVLKKDDVLTVTFDLENTGKYEGTEVAQLYIQDKIGSVTRPVKELKRFTRVTLKPGEKKSVSFELPVSELAFWNIDMAKVVEPGDFGLWVATDSQSGEEVFF
GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGTGCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAATCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCGTTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATTGATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGCAGCTATGGTAGAAGGTTTTCAGGGAGATTCGCTGAATAGTCCTACTTCAATTGCAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAGCCGAAGGAGGACGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAGCGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAATGTACTCCGTGACGAGTGGGGATTCGATGGTTTTGTTGTGACGGACTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGTTCAAAAGAAGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTGAAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCTTATGTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTGAAGAATGATAAGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTAGGTACTTGGATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATTTATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTGAATGATATTGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTTGTCGTACACCACATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATTCGATCTGGAAAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGAAAAAAAGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCTTC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,093 total letters Query= APC115044.116 Length=2541 Score E Sequences producing significant alignments: (Bits) Value wt 3838 0.0 08F_F2_APC115044.116_F02.seq 1104 0.0 02F_F2_APC115044.116_F02.seq 787 0.0 V E S L L S K M T L E E K I G Q M N Q I S S Y G N I E D M S Query_1 202 GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGT 291 2 1 .......................................................................................... 90 A L I K K G E I G S I L N E V D P V R I N A L Q R V A Query_1 292 GCTTTGATTAAGAAAGGTG---AAATC-GGTTCCATCTTG-AATGA-GGTGGATCCGGTGC-GTATTAATGCG-CTACAGCGCGTGGCAA 373 2 91 ...................---.....-............-.....-..............-...........-................ 172 1 731 ...N.N........A.N.TGA.....N..........NNN.....A............N.C..N...T..N.G..N.........N... 643 M E E S R L G I P L L I A R D V I H G F K T I F P I P L G Query_1 374 TGGAAGAAT-CCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGG-ACA 461 2 173 .........-............................................................................-... 260 1 642 N.......NN....N................N.............N......NN.N......N..N...............N....G... 553 Q A A S F N P Q V A K D G A R I A A I E A S S V G I R W T F Query_1 462 AGCGGCTTCGTTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAG-CAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTG 550 2 261 ...............................................-.......................................... 349 1 552 ................................N..............G...........N.............................. 463 A P M I D I A R D P R W G R I A E G C G E D T Y L T S V M G Query_1 551 CGCCAATGATTGATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAG 640 2 350 .......................................................................................... 439 1 462 .A........................................................................................ 373 A A M V E G F Q G D S L N S P T S I A A C P K H F V G Y G A Query_1 641 CAGCTATGGTAGAAGGTTTTCAGGGAGATTCGCTGAATAGTCCTACTTCAATTGCAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAG 730 2 440 .......................................................................................... 529 1 372 ............................................................N............................. 283 A E G G R D Y N S T F I P E R R L R N V Y L P P F E A A T K Query_1 731 CCGAAGGAGGACGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAG 820 2 530 .......................................................................................... 619 1 282 .......................................................................................... 193 1 781 ...........C.. 794 0 29 .....................................N.......N.................................... 110 A G A A T F M T S F N D N D G I P S T G N A F I L K N V L R Query_1 821 CGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAATGTACTCCGTG 910 2 620 .......................................................................................... 709 1 192 ...............N.......................................................................... 103 0 111 ........................N.N.......................................................NNN..... 200 D E W G F D G F V V T D A A S A S E M I S H G F A A G S K Query_1 911 ACGAGTGGGGATTCGATGGTTTTGTTGTGACGGACGCTGCTTCTGCCAGCGAAATGATAA-GCCATGGTTTTGCCGCCGGTTCAAAA-GA 998 2 710 ...................................TGG......................-..........................-.. 797 1 102 ............................................................G.........N.N..............N.. 13 0 201 ..............N................N...............N..N.........-........N.................-N. 288 E V A M K S V N A G V D M E M V S Y T F V K E L P E L V K E Query_1 999 AGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTGAAAGAGGG 1088 2 798 .......................................................................................... 887 1 12 ........ 5 0 289 ......N.....................N.........................................N.N..T...N.........N 378 G K V K E S T I D E A V R N I L R I K Y R L G L F D T P Y V Query_1 1089 AAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCTTATGTAGA 1178 2 888 .......................................................................................... 977 0 379 ..T...................N.........N..-...N...........N.N.NN..N....N.......................N. 467 D E Q Q T S V M Y A P S H L E A A K Q A A V E S A I L L K N Query_1 1179 TGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTGAAGAATGA 1268 2 978 .......................................................................................... 1067 0 468 .........N...N...................NN.....NC.....N...N.........N.......N.............NN... 555 D K E V L P L Q P S V K T V A V V G P M A N A P Y E Q L G T Query_1 1269 TAAGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTAGGTACTTG 1358 2 1068 .......................................................................................... 1157 W I F D G E K A R T Q T P L N A I K E M V G D K V Q V I Y E Query_1 1359 GATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATTTATGAACC 1448 2 1158 .......................................................................................... 1247 P G L A Y S R E K N P A S V A K A A A A A A R A D V I L A F Query_1 1449 GGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTTGT 1538 2 1248 .......................................................................................... 1337 2 1318 ............ 1307 V G E E S I L S G E A H C L A D L D L Q G D Q G A L I T A L Query_1 1539 GGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGC 1628 2 1338 .......................................................................................... 1427 A K T G K P V V T I V M A G R P L T I G K E V E E S T A V L Query_1 1629 TAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTA 1718 2 1428 .......................................................................................... 1517 Y S F H P G T M G G P A L A D L L W G K A V P S G K A P V T Query_1 1719 TTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTT 1808 2 1518 .......................................................................................... 1607 F P R M V G Q I P V Y Y A H N N T G R P A T R N E V L L N D Query_1 1809 CCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTGAATGATAT 1898 2 1608 .......................................................................................... 1697 2 1666 ............ 1655 I A V E A G Q T S L G C T S F Y M D A G F D P L F P F G Y G Query_1 1899 TGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTT 1988 2 1698 .......................................................................................... 1787 L S Y T T F K Y S N I K L A S D V L K K D D V L T V T F D L Query_1 1989 GTCGTACACCACATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATTCGATCTGGA 2078 2 1788 .......................................................................................... 1877 E N T G K Y E G T E V A Q L Y I Q D K I G S V T R P V K E L Query_1 2079 AAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAA 2168 2 1878 .......................................................................................... 1967 2 1926 ............ 1915 K R F T R V T L K P G E K K S V S F E L P V S E L A F W N I Query_1 2169 ACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGaaaaaaaGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTTGGAACATAGA 2258 2 1968 .......................................................................................... 2057 D M A K V V E P G D F G L W V A T D S Q S G E E V F F Query_1 2259 TATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCTTC 2337 2 2058 ............................................................................... 2136 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 10202008 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 4,093 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115044.118 (APC115044.33-744.P(748).W281F.pMCSG68)
VESLLSKMTLEEKIGQMNQISSYGNIEDMSALIKKGEIGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQVAKDGARIAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKNVLRDEWGFDGFVVTDWASASEMISHGFAAGSKEVAMKSVNAGVDMEMVSYTFVKELPELVKEGKVKESTIDEAVRNILRIKYRLGLFDTPYVDEQQTSVMYAPSHLEAAKQAAVESAILLKNDKEVLPLQPSVKTVAVVGPMANAPYEQLGTWIFDGEKARTQTPLNAIKEMVGDKVQVIYEPGLAYSREKNPASVAKAAAAAARADVILAFVGEESILSGEAHCLADLDLQGDQGALITALAKTGKPVVTIVMAGRPLTIGKEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKAPVTFPRMVGQIPVYYAHNNTGRPATRNEVLLNDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNIKLASDVLKKDDVLTVTFDLENTGKYEGTEVAQLYIQDKIGSVTRPVKELKRFTRVTLKPGEKKSVSFELPVSELAFWNIDMAKVVEPGDFGLWVATDSQSGEEVFF
GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGTGCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAATCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCGTTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATTGATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGCAGCTATGGTAGAAGGTTTTCAGGGAGATTCGCTGAATAGTCCTACTTCAATTGCAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAGCCGAAGGAGGACGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAGCGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAATGTACTCCGTGACGAGTGGGGATTCGATGGTTTTGTTGTGACGGACTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGTTCAAAAGAAGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTGAAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCTTATGTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTGAAGAATGATAAGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTAGGTACTTGGATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATTTATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTGAATGATATTGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTTGTCGTACACCACATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATTCGATCTGGAAAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGAAAAAAAGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCTTC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,146 total letters Query= APC115044.118 Length=2541 Score E Sequences producing significant alignments: (Bits) Value wt 3844 0.0 V E S L L S K M T L E E K I G Q M N Q I S S Y G N I E D M S Query_1 202 GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGT 291 2 1 .......................................................................................... 90 A L I K K G E I G S I L N E V D P V R I N A L Q R V A M E E Query_1 292 GCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAA 381 2 91 .......................................................................................... 180 S R L G I P L L I A R D V I H G F K T I F P I P L G Q A A S Query_1 382 TCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCG 471 2 181 .......................................................................................... 270 F N P Q V A K D G A R I A A I E A S S V G I R W T F A P M I Query_1 472 TTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATT 561 2 271 .......................................................................................... 360 D I A R D P R W G R I A E G C G E D T Y L T S V M G A A M V Query_1 562 GATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGCAGCTATGGTA 651 2 361 .......................................................................................... 450 E G F Q G D S L N S P T S I A A C P K H F V G Y G A A E G G Query_1 652 GAAGGTTTTCAGGGAGATTCGCTGAATAGTCCTACTTCAATTGCAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAGCCGAAGGAGGA 741 2 451 .......................................................................................... 540 R D Y N S T F I P E R R L R N V Y L P P F E A A T K A G A A Query_1 742 CGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAGCGGGTGCAGCC 831 2 541 .......................................................................................... 630 T F M T S F N D N D G I P S T G N A F I L K N V L R D E W G Query_1 832 ACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAATGTACTCCGTGACGAGTGGGGA 921 2 631 .......................................................................................... 720 F D G F V V T D F A S A S E M I S H G F A A G S K E V A M K Query_1 922 TTCGATGGTTTTGTTGTGACGGACTTTGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGTTCAAAAGAAGTGGCAATGAAA 1011 2 721 .........................GG............................................................... 810 S V N A G V D M E M V S Y T F V K E L P E L V K E G K V K E Query_1 1012 TCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTGAAAGAGGGAAAGGTGAAGGAA 1101 2 811 .......................................................................................... 900 S T I D E A V R N I L R I K Y R L G L F D T P Y V D E Q Q T Query_1 1102 AGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCTTATGTAGATGAACAACAAACA 1191 2 901 .......................................................................................... 990 S V M Y A P S H L E A A K Q A A V E S A I L L K N D K E V L Query_1 1192 TCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTGAAGAATGATAAGGAAGTGTTG 1281 2 991 .......................................................................................... 1080 P L Q P S V K T V A V V G P M A N A P Y E Q L G T W I F D G Query_1 1282 CCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTAGGTACTTGGATATTTGATGGT 1371 2 1081 .......................................................................................... 1170 E K A R T Q T P L N A I K E M V G D K V Q V I Y E P G L A Y Query_1 1372 GAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATTTATGAACCGGGACTAGCATAT 1461 2 1171 .......................................................................................... 1260 S R E K N P A S V A K A A A A A A R A D V I L A F V G E E S Query_1 1462 AGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAATCT 1551 2 1261 .......................................................................................... 1350 2 1318 ............ 1307 I L S G E A H C L A D L D L Q G D Q G A L I T A L A K T G K Query_1 1552 ATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGCTAAGACGGGTAAA 1641 2 1351 .......................................................................................... 1440 P V V T I V M A G R P L T I G K E V E E S T A V L Y S F H P Query_1 1642 CCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTATTCATTCCATCCG 1731 2 1441 .......................................................................................... 1530 G T M G G P A L A D L L W G K A V P S G K A P V T F P R M V Query_1 1732 GGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTTCCCGAGGATGGTG 1821 2 1531 .......................................................................................... 1620 G Q I P V Y Y A H N N T G R P A T R N E V L L N D I A V E A Query_1 1822 GGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTGAATGATATTGCTGTTGAGGCA 1911 2 1621 .......................................................................................... 1710 2 1666 ............ 1655 G Q T S L G C T S F Y M D A G F D P L F P F G Y G L S Y T T Query_1 1912 GGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTTGTCGTACACCACA 2001 2 1711 .......................................................................................... 1800 F K Y S N I K L A S D V L K K D D V L T V T F D L E N T G K Query_1 2002 TTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATTCGATCTGGAAAATACCGGGAAA 2091 2 1801 .......................................................................................... 1890 Y E G T E V A Q L Y I Q D K I G S V T R P V K E L K R F T R Query_1 2092 TATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCGT 2181 2 1891 .......................................................................................... 1980 2 1926 ............ 1915 V T L K P G E K K S V S F E L P V S E L A F W N I D M A K V Query_1 2182 GTGACATTGAAGCCGGGTGAGaaaaaaaGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGTT 2271 2 1981 .......................................................................................... 2070 V E P G D F G L W V A T D S Q S G E E V F F Query_1 2272 GTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCTTC 2337 2 2071 .................................................................. 2136 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5297450 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 2,146 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115044.126 (APC115044.33-744.P(748).K201A.pMCSG68)
VESLLSKMTLEEKIGQMNQISSYGNIEDMSALIKKGEIGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQVAKDGARIAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKNVLRDEWGFDGFVVTDWASASEMISHGFAAGSKEVAMKSVNAGVDMEMVSYTFVKELPELVKEGKVKESTIDEAVRNILRIKYRLGLFDTPYVDEQQTSVMYAPSHLEAAKQAAVESAILLKNDKEVLPLQPSVKTVAVVGPMANAPYEQLGTWIFDGEKARTQTPLNAIKEMVGDKVQVIYEPGLAYSREKNPASVAKAAAAAARADVILAFVGEESILSGEAHCLADLDLQGDQGALITALAKTGKPVVTIVMAGRPLTIGKEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKAPVTFPRMVGQIPVYYAHNNTGRPATRNEVLLNDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNIKLASDVLKKDDVLTVTFDLENTGKYEGTEVAQLYIQDKIGSVTRPVKELKRFTRVTLKPGEKKSVSFELPVSELAFWNIDMAKVVEPGDFGLWVATDSQSGEEVFF
GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGTGCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAATCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCGTTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATTGATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGCAGCTATGGTAGAAGGTTTTCAGGGAGATTCGCTGAATAGTCCTACTTCAATTGCAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAGCCGAAGGAGGACGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAGCGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAATGTACTCCGTGACGAGTGGGGATTCGATGGTTTTGTTGTGACGGACTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGTTCAAAAGAAGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTGAAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCTTATGTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTGAAGAATGATAAGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTAGGTACTTGGATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATTTATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTGAATGATATTGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTTGTCGTACACCACATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATTCGATCTGGAAAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGAAAAAAAGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCTTC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,839 total letters Query= APC115044.126 Length=2541 Score E Sequences producing significant alignments: (Bits) Value wt 3838 0.0 08H_H2_APC115044.126_H02.seq 1386 0.0 02H_H2_APC115044.126_H02.seq 1223 0.0 Q F E K G T E N L Y F Q S N A V E S L L S K M T L E Query_1 157 CAGTTCGAAAAGGG-TACCG-AGAA-CCTGTACTTCC-AAT--CCAATGCC-GTAGAA--TCTCTCC-TGTCTA-AGATGACCCTTGAGG 235 2 1 ......--.......-......-............... 34 1 888 ..N..NA.......N..NN.G....N...........C...TC........C......ATN......CN..T..N...G.N......N.. 799 E K I G Q M N Q I S S Y G N I E D M S A L I K K G E I G Query_1 236 AGAAAA-TCGGT-CAGATGAACCAGATTT-CCTCTTACGGTAAT-ATCGAGGATATGAGTGCTTT-GATT-AAGAAAGGTGAAATCGGTT 319 2 35 ......-.....-................-..............-....................-....-................... 118 1 798 ......A.....T................T........N.....T....................N....T................... 709 S I L N E V D P V R I N A L Q R V A M E E S R L G I P L L I Query_1 320 CCATCTTGAATGAGGTGGATCCGGTGC-GTATTAATGCGCTACAGCGCGTGGCAATGGAAGAATCCCGTTTGGGTATTCCTTTATTGATA 408 2 119 ...........................-.............................................................. 207 1 708 ...............N.N.........C.............................................................. 619 A R D V I H G F K T I F P I P L G Q A A S F N P Q V A K D G Query_1 409 GCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCGTTCAATCCGCAGGTAGCGAAAGACGGT 498 2 208 .......................................................................................... 297 1 618 .......................................................................................... 529 A R I A A I E A S S V G I R W T F A P M I D I A R D P R W G Query_1 499 GCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATTGATATTGCCCGCGATCCTCGCTGGGGA 588 2 298 .......................................................................................... 387 1 528 .....................................................A.................................... 439 R I A E G C G E D T Y L T S V M G A A M V E G F Q G D S L N Query_1 589 CGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGCAGCTATGGTAGAAGGTTTTCAGGGAGATTCGCTGAAT 678 2 388 .......................................................................................... 477 1 438 .......................................................................................... 349 0 9 ..........N.-............................................ 64 S P T S I A A C P A H F V G Y G A A E G G R D Y N S T F I P Query_1 679 AGTCCTACTTCAATTGCAGCTTGCCCTGCTCATTTTGTAGGTTACGGTGCAGCCGAAGGAGGACGTGATTATAATTCCACGTTCATTCCC 768 2 478 ...........................AAA............................................................ 567 1 348 .......................................................................................... 259 0 65 ......N................................................................................... 154 E R R L R N V Y L P P F E A A T K A G A A T F M T S F N D N Query_1 769 GAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAGCGGGTGCAGCCACGTTTATGACTTCATTTAATGATAAT 858 2 568 .......................................................................................... 657 1 258 .......................................................................................... 169 0 155 .......................................................................................... 244 D G I P S T G N A F I L K N V L R D E W G F D G F V V T D W Query_1 859 GATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAATGTACTCCGTGACGAGTGGGGATTCGATGGTTTTGTTGTGACGGACTGG 948 2 658 .......................................................................................... 747 1 168 .......................................................................................... 79 0 245 .......................................................................................... 334 A S A S E M I S H G F A A G S K E V A M K S V N A G V D M E Query_1 949 GCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGTTCAAAAGAAGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAA 1038 2 748 .......................................................................................... 837 1 78 .................................................................. 13 0 335 ...........................................................................N.............. 424 M V S Y T F V K E L P E L V K E G K V K E S T I D E A V R N Query_1 1039 ATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTGAAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAAT 1128 2 838 .......................................................................................... 927 0 425 .......................................................................................... 514 I L R I K Y R L G L F D T P Y V D E Q Q T S V M Y A P S H L Query_1 1129 ATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCTTATGTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTG 1218 2 928 .......................................................................................... 1017 0 515 ...................N.N..N............................N..................N................. 604 0 1166 ............ 1155 E A A K Q A A V E S A I L L K N D K E V L P L Q P S V K T Query_1 1219 GAAGCAG-CTAA-GCAAGCCGCTGTTGAATCGGCTATTCTGTTGAAGAATGATAAGGAAGTGTTGCCGTTACAGCCATC-TGTGAAAACT 1305 2 1018 .......-....-..................................................................-.......... 1104 0 605 .......C....CC..N....N...................N.............N.............NN.NN.....N.......... 694 V A V V G P M A N A P Y E Q L G T W I F D G E K A R T Query_1 1306 GTTGCAGTGGTAGGACCTATGG-CTAATG-CACCT-TATGAACAG-TTAGGTACTTGG-ATATTT-GATGGTGAG-AAAGCTCG-TACTC 1387 2 1105 ......................-......-.....-.........-............-......-.........-........-..... 1186 0 695 .N....NN...........N..C......C.....N..A......N...N.....N..N....N.G.........A........NN.... 784 Q T P L N A I K E M V G D K V Q V I Y E P G L A Y S R E K Query_1 1388 AGA-CTCCGTT-GAACGCTATTAAAG-AAATGGTTGGCGATAAAGTACAGGTGATTTATGAACCGGGACTAGCATATAGTCGTGAGAAAA 1474 2 1187 ...-.......-..............-............................................................... 1273 0 785 .A.N......NG....N.NT......N...... 817 N P A S V A K A A A A A A R A D V I L A F V G E E S I L S G Query_1 1475 ATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTG 1564 2 1274 .......................................................................................... 1363 2 1318 ............ 1307 E A H C L A D L D L Q G D Q G A L I T A L A K T G K P V V T Query_1 1565 AAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGCTAAGACGGGTAAACCTGTAGTGACTA 1654 2 1364 .......................................................................................... 1453 I V M A G R P L T I G K E V E E S T A V L Y S F H P G T M G Query_1 1655 TTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTATTCATTCCATCCGGGCACAATGGGCG 1744 2 1454 .......................................................................................... 1543 G P A L A D L L W G K A V P S G K A P V T F P R M V G Q I P Query_1 1745 GTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTTCCCGAGGATGGTGGGACAAATTCCTG 1834 2 1544 .......................................................................................... 1633 V Y Y A H N N T G R P A T R N E V L L N D I A V E A G Q T S Query_1 1835 TGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTGAATGATATTGCTGTTGAGGCAGGACAGACTTCAC 1924 2 1634 .......................................................................................... 1723 2 1666 ............ 1655 L G C T S F Y M D A G F D P L F P F G Y G L S Y T T F K Y S Query_1 1925 TGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTTGTCGTACACCACATTTAAGTATAGCA 2014 2 1724 .......................................................................................... 1813 N I K L A S D V L K K D D V L T V T F D L E N T G K Y E G T Query_1 2015 ACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATTCGATCTGGAAAATACCGGGAAATATGAAGGAACGG 2104 2 1814 .......................................................................................... 1903 E V A Q L Y I Q D K I G S V T R P V K E L K R F T R V T L K Query_1 2105 AAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCGTGTGACATTGAAGC 2194 2 1904 .......................................................................................... 1993 2 1926 ............ 1915 P G E K K S V S F E L P V S E L A F W N I D M A K V V E P G Query_1 2195 CGGGTGAGaaaaaaaGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGTTGTGGAACCCGGAG 2284 2 1994 .......................................................................................... 2083 D F G L W V A T D S Q S G E E V F F Query_1 2285 ACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCTTC 2337 2 2084 ..................................................... 2136 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12084912 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 4,839 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115044.127 (APC115044.33-744.P(748).H202A.pMCSG68)
VESLLSKMTLEEKIGQMNQISSYGNIEDMSALIKKGEIGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQVAKDGARIAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKNVLRDEWGFDGFVVTDWASASEMISHGFAAGSKEVAMKSVNAGVDMEMVSYTFVKELPELVKEGKVKESTIDEAVRNILRIKYRLGLFDTPYVDEQQTSVMYAPSHLEAAKQAAVESAILLKNDKEVLPLQPSVKTVAVVGPMANAPYEQLGTWIFDGEKARTQTPLNAIKEMVGDKVQVIYEPGLAYSREKNPASVAKAAAAAARADVILAFVGEESILSGEAHCLADLDLQGDQGALITALAKTGKPVVTIVMAGRPLTIGKEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKAPVTFPRMVGQIPVYYAHNNTGRPATRNEVLLNDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNIKLASDVLKKDDVLTVTFDLENTGKYEGTEVAQLYIQDKIGSVTRPVKELKRFTRVTLKPGEKKSVSFELPVSELAFWNIDMAKVVEPGDFGLWVATDSQSGEEVFF
GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGTGCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAATCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCGTTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATTGATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGCAGCTATGGTAGAAGGTTTTCAGGGAGATTCGCTGAATAGTCCTACTTCAATTGCAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAGCCGAAGGAGGACGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAGCGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAATGTACTCCGTGACGAGTGGGGATTCGATGGTTTTGTTGTGACGGACTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGTTCAAAAGAAGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTGAAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCTTATGTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTGAAGAATGATAAGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTAGGTACTTGGATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATTTATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTGAATGATATTGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTTGTCGTACACCACATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATTCGATCTGGAAAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGAAAAAAAGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCTTC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,651 total letters Query= APC115044.127 Length=2541 Score E Sequences producing significant alignments: (Bits) Value wt 3844 0.0 03A_A3_APC115044.127_A03.seq 1880 0.0 09A_A3_APC115044.127_A03.seq 1808 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAA 90 1 1033 ...............................................N.-...-........-..................-........ 948 1 990 ...........A... 1004 E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E N Query_1 91 GAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAAC 180 1 947 .......................................................................................... 858 L Y F Q S N A V E S L L S K M T L E E K I G Q M N Q I S S Y Query_1 181 CTGTACTTCCAATCCAATGCCGTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTAC 270 2 1 ..................................................................... 69 1 857 .......................................................................................... 768 G N I E D M S A L I K K G E I G S I L N E V D P V R I N A L Query_1 271 GGTAATATCGAGGATATGAGTGCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTA 360 2 70 .......................................................................................... 159 1 767 .......................................................................................... 678 Q R V A M E E S R L G I P L L I A R D V I H G F K T I F P I Query_1 361 CAGCGCGTGGCAATGGAAGAATCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATT 450 2 160 .......................................................................................... 249 1 677 .......................................................................................... 588 P L G Q A A S F N P Q V A K D G A R I A A I E A S S V G I R Query_1 451 CCCTTGGGACAAGCGGCTTCGTTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGG 540 2 250 .......................................................................................... 339 1 587 .......................................................................................... 498 W T F A P M I D I A R D P R W G R I A E G C G E D T Y L T S Query_1 541 TGGACTTTTGCGCCAATGATTGATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCC 630 2 340 .......................................................................................... 429 0 25 .... 28 1 497 ...........A.............................................................................. 408 V M G A A M V E G F Q G D S L N S P T S I A A C P K A F V G Query_1 631 GTAATGGGAGCAGCTATGGTAGAAGGTTTTCAGGGAGATTCGCTGAATAGTCCTACTTCAATTGCAGCTTGCCCTAAAGCTTTTGTAGGT 720 2 430 ..............................................................................CA.......... 519 0 29 ...-...................................................................................... 117 1 407 .......................................................................................... 318 Y G A A E G G R D Y N S T F I P E R R L R N V Y L P P F E A Query_1 721 TACGGTGCAGCCGAAGGAGGACGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCT 810 2 520 .......................................................................................... 609 0 118 .......................................................................................... 207 1 317 .......................................................................................... 228 A T K A G A A T F M T S F N D N D G I P S T G N A F I L K N Query_1 811 GCCACCAAAGCGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAAT 900 2 610 .......................................................................................... 699 0 208 .......................................................................................... 297 1 227 .......................................................................................... 138 V L R D E W G F D G F V V T D W A S A S E M I S H G F A A G Query_1 901 GTACTCCGTGACGAGTGGGGATTCGATGGTTTTGTTGTGACGGACTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGT 990 2 700 .......................................................................................... 789 0 298 .......................................................................................... 387 1 137 .......................................................................................... 48 S K E V A M K S V N A G V D M E M V S Y T F V K E L P E L V Query_1 991 TCAAAAGAAGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTG 1080 2 790 .......................................................................................... 879 0 388 .......................................................................................... 477 1 47 .........................N........... 11 K E G K V K E S T I D E A V R N I L R I K Y R L G L F D T P Query_1 1081 AAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCT 1170 2 880 .......................................................................................... 969 0 478 .......................................................................................... 567 Y V D E Q Q T S V M Y A P S H L E A A K Q A A V E S A I L L Query_1 1171 TATGTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTG 1260 2 970 .......................................................................................... 1059 0 568 .......................................................................................... 657 K N D K E V L P L Q P S V K T V A V V G P M A N A P Y E Q L Query_1 1261 AAGAATGATAAGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTA 1350 2 1060 .......................................................................................... 1149 0 658 .......................................................................................... 747 G T W I F D G E K A R T Q T P L N A I K E M V G D K V Q V I Query_1 1351 GGTACTTGGATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATT 1440 2 1150 .......................................................................................... 1239 0 748 ...................................................................N...................... 837 Y E P G L A Y S R E K N P A S V A K A A A A A A R A D V I L Query_1 1441 TATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTT 1530 2 1240 .......................................................................................... 1329 2 1318 ............ 1307 0 838 .......................................................................................... 927 0 916 ............ 905 A F V G E E S I L S G E A H C L A D L D L Q G D Q G A L I Query_1 1531 GCTTTTGTGGGTGAAGAATCTATTC-TTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTAC 1619 2 1330 .........................-................................................................ 1418 0 928 .........................T...................-....................-...................-... 1014 T A L A K T G K P V V T I V M A G R P L T I G K E V E E S T Query_1 1620 AGCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGC 1709 2 1419 .......................................................................................... 1508 0 1015 .....G....-......--T.........................-.............NNN.....-...........NNN.....N.. 1099 A V L Y S F H P G T M G G P A L A D L L W G K A V P S G K A Query_1 1710 TGTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCC 1799 2 1509 .......................................................................................... 1598 0 1100 ........-N......AT...N...N.......-.CNNNN............ 1149 P V T F P R M V G Q I P V Y Y A H N N T G R P A T R N E V L Query_1 1800 GGTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCT 1889 2 1599 .......................................................................................... 1688 2 1666 ............ 1655 L N D I A V E A G Q T S L G C T S F Y M D A G F D P L F P F Query_1 1890 GAATGATATTGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGG 1979 2 1689 .......................................................................................... 1778 G Y G L S Y T T F K Y S N I K L A S D V L K K D D V L T V T Query_1 1980 TTATGGCTTGTCGTACACCACATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATT 2069 2 1779 .......................................................................................... 1868 F D L E N T G K Y E G T E V A Q L Y I Q D K I G S V T R P V Query_1 2070 CGATCTGGAAAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAA 2159 2 1869 .......................................................................................... 1958 2 1926 ............ 1915 K E L K R F T R V T L K P G E K K S V S F E L P V S E L A F Query_1 2160 AGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGaaaaaaaGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTTG 2249 2 1959 .......................................................................................... 2048 W N I D M A K V V E P G D F G L W V A T D S Q S G E E V F F Query_1 2250 GAACATAGATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCTTC 2337 2 2049 ........................................................................................ 2136 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11610400 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 4,651 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115044.132 (APC115044.33-744.P(748).E487Q.pMCSG68)
VESLLSKMTLEEKIGQMNQISSYGNIEDMSALIKKGEIGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQVAKDGARIAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKNVLRDEWGFDGFVVTDWASASEMISHGFAAGSKEVAMKSVNAGVDMEMVSYTFVKELPELVKEGKVKESTIDEAVRNILRIKYRLGLFDTPYVDEQQTSVMYAPSHLEAAKQAAVESAILLKNDKEVLPLQPSVKTVAVVGPMANAPYEQLGTWIFDGEKARTQTPLNAIKEMVGDKVQVIYEPGLAYSREKNPASVAKAAAAAARADVILAFVGEESILSGEAHCLADLDLQGDQGALITALAKTGKPVVTIVMAGRPLTIGKEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKAPVTFPRMVGQIPVYYAHNNTGRPATRNEVLLNDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNIKLASDVLKKDDVLTVTFDLENTGKYEGTEVAQLYIQDKIGSVTRPVKELKRFTRVTLKPGEKKSVSFELPVSELAFWNIDMAKVVEPGDFGLWVATDSQSGEEVFF
GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGTGCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAATCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCGTTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATTGATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGCAGCTATGGTAGAAGGTTTTCAGGGAGATTCGCTGAATAGTCCTACTTCAATTGCAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAGCCGAAGGAGGACGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAGCGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAATGTACTCCGTGACGAGTGGGGATTCGATGGTTTTGTTGTGACGGACTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGTTCAAAAGAAGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTGAAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCTTATGTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTGAAGAATGATAAGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTAGGTACTTGGATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATTTATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTGAATGATATTGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTTGTCGTACACCACATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATTCGATCTGGAAAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGAAAAAAAGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCTTC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,535 total letters Query= APC115044.132 Length=2541 Score E Sequences producing significant alignments: (Bits) Value wt 3844 0.0 09B_B3_APC115044.132_B03.seq 1788 0.0 03B_B3_APC115044.132_B03.seq 1741 0.0 V E S L L S K M T L E E K I G Q M N Q I S S Y G N I E D M S Query_1 202 GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGT 291 2 1 .......................................................................................... 90 A L I K K G E I G S I L N E V D P V R I N A L Q R V A M E E Query_1 292 GCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAA 381 2 91 .......................................................................................... 180 1 946 ............ 935 S R L G I P L L I A R D V I H G F K T I F P I P L G Q A A S Query_1 382 TCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCG 471 2 181 .......................................................................................... 270 F N P Q V A K D G A R I A A I E A S S V G I R W T F A P M I Query_1 472 TTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATT 561 2 271 .......................................................................................... 360 D I A R D P R W G R I A E G C G E D T Y L T S V M G A A M V Query_1 562 GATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGCAGCTATGGTA 651 2 361 .......................................................................................... 450 E G F Q G D S L N S P T S I A A C P K H F V G Y G A A E G G Query_1 652 GAAGGTTTTCAGGGAGATTCGCTGAATAGTCCTACTTCAATTGCAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAGCCGAAGGAGGA 741 2 451 .......................................................................................... 540 R D Y N S T F I P E R R L R N V Y L P P F E A A T K A G A Query_1 742 CGTGATTATAATTCCACGTTCATTCCCGAA-CGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAGCGGGTGCAGC 830 2 541 ..............................-........................................................... 629 1 1194 .....-.............N.T.G..NNN....NNN..-..NNN..N.G.......ANNN.N..A.......A...... 1118 A T F M T S F N D N D G I P S T G N A F I L K N V L R D E W Query_1 831 CACGTTTATGACTTCATTTAATGATAATGATGGAAT-CCCTTCTACCGGGAATGCTTTT-ATTTTGAAGAATGTACTCCGTGACGAGTGG 918 2 630 ....................................-......................-.............................. 717 1 1117 ..N...N.-....G....-...AN...N........T..N....N.........N....TN..N...G....NNCT...NNC-...ANN. 1031 G F D G F V V T D W A S A S E M I S H G F A A G S K E V Query_1 919 GGATTCGA-TGGTTTTGTTGTGA--CGGACTGGGCTTCTGCCAGCGAAATGATAAG-CCATGGTTTTGCCGCCGGTTCAAAA-GAAGTGG 1003 2 718 ........-..............--...............................-.........................-....... 802 1 1030 .N......N...........NN.GCN..................N......N....N.....--..................A....... 943 A M K S V N A G V D M E M V S Y T F V K E L P E L V K E G Query_1 1004 CAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGG-AACTGCCGGAATTAGTGAAA-GAGGGAAA 1091 2 803 ............................................................-....................-........ 890 1 942 .....G..............................................N.......N...NN...............A........ 853 K V K E S T I D E A V R N I L R I K Y R L G L F D T P Y V D Query_1 1092 GGTGAAGGAAAGCACTATCGATGAGGCTGTTCGT-AATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCTTATGTAGATG 1180 2 891 ..................................-....................................................... 979 1 852 ..................................N....................................................N.. 763 E Q Q T S V M Y A P S H L E A A K Q A A V E S A I L L K N D Query_1 1181 AACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTGAAGAATGATA 1270 2 980 .......................................................................................... 1069 1 762 .......................................................................................... 673 0 31 ....... 37 K E V L P L Q P S V K T V A V V G P M A N A P Y E Q L G T W Query_1 1271 AGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTAGGTACTTGGA 1360 2 1070 .......................................................................................... 1159 1 672 .......................................................................................... 583 0 38 .......................................................................................... 127 I F D G E K A R T Q T P L N A I K E M V G D K V Q V I Y E P Query_1 1361 TATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATTTATGAACCGG 1450 2 1160 .......................................................................................... 1249 1 582 .......................................................................................... 493 0 128 .......................................................................................... 217 G L A Y S R E K N P A S V A K A A A A A A R A D V I L A F V Query_1 1451 GACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGG 1540 2 1250 .......................................................................................... 1339 2 1318 ............ 1307 1 492 .......................................................................................... 403 1 424 ............ 435 0 218 .......................................................................................... 307 0 286 ............ 275 G E E S I L S G Q A H C L A D L D L Q G D Q G A L I T A L A Query_1 1541 GTGAAGAATCTATTCTTTCGGGTCAGGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGCTA 1630 2 1340 .......................G.A................................................................ 1429 1 402 .......................................................................................... 313 0 308 .......................................................................................... 397 K T G K P V V T I V M A G R P L T I G K E V E E S T A V L Y Query_1 1631 AGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTATT 1720 2 1430 .......................................................................................... 1519 1 312 .......................................................................................... 223 0 398 .......................................................................................... 487 S F H P G T M G G P A L A D L L W G K A V P S G K A P V T F Query_1 1721 CATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTTCC 1810 2 1520 .......................................................................................... 1609 1 222 .......................................................................................... 133 0 488 .......................................................................................... 577 P R M V G Q I P V Y Y A H N N T G R P A T R N E V L L N D I Query_1 1811 CGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTGAATGATATTG 1900 2 1610 .......................................................................................... 1699 2 1666 ............ 1655 1 132 .......................................................................................... 43 1 76 ............ 87 0 578 .......................................................................................... 667 0 634 ............ 623 A V E A G Q T S L G C T S F Y M D A G F D P L F P F G Y G L Query_1 1901 CTGTTGAGGCAGGACAGACTTCA-CTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTTG 1989 2 1700 .......................-.................................................................. 1788 1 42 ...................N...N.N.... 13 0 668 .......................-.................................................................. 756 S Y T T F K Y S N I K L A S D V L K K D D V L T V T F D L Query_1 1990 TCGTACACCACA-TTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATTCGATCTGGA 2078 2 1789 ............-............................................................................. 1877 0 757 ............T.......................................-..................................-.. 844 E N T G K Y E G T E V A Q L Y I Q D K I G S V T R P V K E L Query_1 2079 AAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAA 2168 2 1878 .......................................................................................... 1967 2 1926 ............ 1915 0 845 ..........-..N.....................-................-........................N.....-...... 930 0 891 ............ 880 K R F T R V T L K P G E K K S V S F E L P V S E L A F W N I Query_1 2169 ACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGaaaaaaaGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGC-ATTTTGGAACATAG 2257 2 1968 ...........................................................................-.............. 2056 0 931 ...-N.N..........NT.....NN.......-N..............-..N...........NN..C......NN.....NT..N..C 1017 D M A K V V E P G D F G L W V A T D S Q S G E E V F F * H Query_1 2258 ATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACG-GATAG-TCAG-TCCGGAGAAGAAGTTTTCTTCTAACATT 2344 2 2057 ..................................................-.....-....-..................... 2136 0 1018 .....N.........N.............-......N....T.......NA.....C....T......-..............N...N.. 1105 W K W I T D P N Query_1 2345 GG-AAGTGGATAACGGAT-CCGAAT 2367 0 1106 ..N..NN..NNN......A..N... 1130 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11317616 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 4,535 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115045.104 (APC115045.26-772.P(773).D292A.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVSGEIVTGQAQNSDVAKKIEQGLVGGLLNLKGVEKIRDVQKLAIEKSRLGIPLIFGMDVVHGYETIFPIPLGLSCSWDMEAIRKSARVAAIEASADGISWTFSPMVDISRDPRWGRVSEGNGEDPFLGGAIAKAMVSGYQGIDLNNQLKRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEYMYPYQAAVDAGVGSVMASFNEIDGIPATANKWLMTDVLRKQWGFDGFVVTDFTGISEMIAHGIGDLQTVSARALNAGVDMDMVSEGFTGTIKKSIDEGKISMETLDKACRRILEAKYKLGLFDNPYKYCDLKRPKRDIFTKEHRDAARKIAGESFVLLKNDKSGSSANPTLPLKKEGTVAVIGPLANTRSNMPGTWSVAARLNDYPSVYEGLKEMMKGKVNITYAKGSNLISDAAYEERATMFGRSLNRDNRTDKEMLDEALKVAANADVIIAALGESSEMSGESSSRTNLALPDVQRTLLEALLKTGKPVVLTLFTGRPLTLTWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLLEGKWFEKFRSNYLDVDNDPLYPFGYGLSYTNFQYSDITLSAPTMGQDGSVTAMVTVTNTGKYDGAEVVQLYIRDLVGSITRPVKELKGFDKIFLKAGESKTVSFKITPELLRFYDYELNYVAEPGDFDIMIGGNSQSVKTTHLSL
AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTTTCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAAGGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCATGGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATCGAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAACGGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAACGATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAACCGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGCATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGTATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGTGAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAAGCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGCGACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAAAAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGACTATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCAGCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,996 total letters Query= APC115045.104 Length=2646 Score E Sequences producing significant alignments: (Bits) Value wt 4033 0.0 03C_C3_APC115045.104_C03.seq 1424 0.0 09C_C3_APC115045.104_C03.seq 1317 0.0 H S S G V D L W S H P Q F E K G T E N L Y F Q S N A Query_1 122 ATCATTCTTCTGGTGTAGA--TCTGTGGTCTCAT-CCGCAGTTCG-AAAAGGG-TACCGAG-AACCTG--TACTTCCAA--TCCAATGCC 201 1 1014 ......N..N.....G.A.AN.NN.....T.T..T.N......T.N.......N.NN..NNN.....TGT..N..N.N.AT.....N... 925 K S P Q D M D R F I D A L M K K M T V E E K I G Q Query_1 202 AAG-TCTCCGC-AGGACATGGATC--GCTTCATCGA-TGCATT--GA-TGAAGAAGATGACTG-TAGAGG--AAAAGATC-GGTCAGCT- 278 2 1 ...-.......-............--..........-......--..-...............-......--........-........- 77 1 924 N.NN.N.N...C.....N.....NNG.T.....GN.N...N..TTN.CN.....NN..T..C.G......GA.......TA...N..NNT 835 L N L P V S G E I V T G Q A Q N S D V A K K I E Q G L V Query_1 279 -GAACCTACCCGTTT--CCGGCGAGATCGTCACCGGGCAGGCACAAAA--CAGCGATGTGGCAAAAAA-GATT-GAACAAGGGCTCGTGG 361 2 78 -..............--...............................--..................-....-................ 160 1 834 G....T..A..NN..TN....T.....NT...............N...NT.......N.N........N....T........TN...... 745 G G L L N L K G V E K I R D V Q K L A I E K S R L G I P L Query_1 362 GCGG-ACTCC-TCAACCTGAAAGGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGAT 449 2 161 ....-.....-............................................................................... 248 1 744 ....G.N...C...............N.................N...........................N................. 655 I F G M D V V H G Y E T I F P I P L G L S C S W D M E A I R Query_1 450 ATTCGGCATGGACGTAGTGCATGGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAA 539 2 249 .......................................................................................... 338 1 654 ............N............................................................................. 565 K S A R V A A I E A S A D G I S W T F S P M V D I S R D P R Query_1 540 ATCTGCCCGCGTTGCAGCCATCGAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTG 629 2 339 .......................................................................................... 428 1 564 .....................N.................................................................... 475 W G R V S E G N G E D P F L G G A I A K A M V S G Y Q G I D Query_1 630 GGGACGCGTCAGCGAGGGTAACGGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCT 719 2 429 .......................................................................................... 518 0 1 .....N............................. 35 1 474 .......................................................................................... 385 L N N Q L K R N D E I M A C V K H F A L Y G A G E A G R D Y Query_1 720 CAACAACCAACTGAAGCGCAACGATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAA 809 2 519 .......................................................................................... 608 0 36 ......................N................................................................... 125 1 384 .......................................................................................... 295 N T V D M S R N R M F N E Y M Y P Y Q A A V D A G V G S V M Query_1 810 TACCGTAGATATGAGTCGTAACCGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGC 899 2 609 .......................................................................................... 698 0 126 .......................................................................................... 215 1 294 .......................................................................................... 205 A S F N E I D G I P A T A N K W L M T D V L R K Q W G F D G Query_1 900 GTCTTTCAACGAAATAGACGGCATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTT 989 2 699 .......................................................................................... 788 0 216 .......................................................................................... 305 1 204 .......................................................................................... 115 F V V T A F T G I S E M I A H G I G D L Q T V S A R A L N Query_1 990 TGTGGTGACAGCTTTTACCGGTATCTCCGAAATGATA-GCGCACGGCATCGGTGACTTGCAGACTGTTTCCG-CA-CGTGCACTCAATGC 1076 2 789 ...........AC........................-..................................-..-.............. 875 0 306 .....................................-..................................-..-.............. 392 1 114 .....................................T..................................A..G...........GN. 25 A G V D M D M V S E G F T G T I K K S I D E G K I S M E T L Query_1 1077 A-GGCGTGGATATGGACATGGTAAGTGAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGG 1165 2 876 .-........................................................................................ 964 0 393 .-........................................................................................ 481 1 24 .NNN..NN......T.. 8 D K A C R R I L E A K Y K L G L F D N P Y K Y C D L K R P K Query_1 1166 ACAAAGCCTGTCGCCGCATCCTTGAAGCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGC 1255 2 965 .......................................................................................... 1054 0 482 .......................................................................................... 571 R D I F T K E H R D A A R K I A G E S F V L L K N D K S G S Query_1 1256 GTGACATCTTCACCAAGGAACATCGCGACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCT 1345 2 1055 .......................................................................................... 1144 0 572 ........................................N.........N....................................... 661 S A N P T L P L K K E G T V A V I G P L A N T R S N M P G T Query_1 1346 CTGCAAACCCAACACTTCCTTTGAAAAAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCT 1435 2 1145 .......................................................................................... 1234 0 662 .............................N...............................N.......N.N.................. 751 W S V A A R L N D Y P S V Y E G L K E M M K G K V N I Query_1 1436 GGAGTGTAGCCGCACG-CCT-CAACGACTA-TCCTTCTGTGTACGA-AGGATT-GAAAGAGATG-ATGAAA-GGCAA-GGTAAACAT-CA 1516 2 1235 ................-...-.........-...............-......-..........-......-.....-.........-.. 1315 0 752 ........N..N....N...N.........T...............TG.....G....C.C...A......N.....N.N.....C.C.. 841 T Y A K G S N L I S D A A Y E E R A T M F G R S L N R Query_1 1517 C-TTATGCC--AAAGGTAG-TAACCT-CATCAG-TGATGCAGCC-TACGAAGAA-CGTGCCACAATGTT-CGGCCGTTCATTAAAT-CGT 1596 2 1316 .-.......--........-......-......-..........-.........-..............-................-... 1395 0 842 .N.......CA....N.N.N..N...N.N...NT..........T.N.N.....N.NGN..C.....N.C...N.....N......C... 931 D N R T D K E M L D E A L K V A A N A D V I I A A L G E S S Query_1 1597 GATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCT 1686 2 1396 .......................................................................................... 1485 0 932 N.A.... 938 E M S G E S S S R T N L A L P D V Q R T L L E A L L K T G K Query_1 1687 GAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAG 1776 2 1486 .......................................................................................... 1575 P V V L T L F T G R P L T L T W E Q E H V P A I L N V W F G Query_1 1777 CCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGT 1866 2 1576 .......................................................................................... 1665 G S E A A Y A I G D V L F G D V N P S G K L T M T F P K N V Query_1 1867 GGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTA 1956 2 1666 .......................................................................................... 1755 G Q I P L F Y N H K N T G R P L L E G K W F E K F R S N Y L Query_1 1957 GGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTG 2046 2 1756 .......................................................................................... 1845 D V D N D P L Y P F G Y G L S Y T N F Q Y S D I T L S A P T Query_1 2047 GATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACT 2136 2 1846 .......................................................................................... 1935 M G Q D G S V T A M V T V T N T G K Y D G A E V V Q L Y I R Query_1 2137 ATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGT 2226 2 1936 .......................................................................................... 2025 D L V G S I T R P V K E L K G F D K I F L K A G E S K T V S Query_1 2227 GACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCT 2316 2 2026 .......................................................................................... 2115 F K I T P E L L R F Y D Y E L N Y V A E P G D F D I M I G G Query_1 2317 TTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGG 2406 2 2116 .......................................................................................... 2205 N S Q S V K T T H L S L Query_1 2407 AACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG 2442 2 2206 .................................... 2241 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 13000405 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 4,996 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115045.105 (APC115045.26-772.P(773).E525A.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVSGEIVTGQAQNSDVAKKIEQGLVGGLLNLKGVEKIRDVQKLAIEKSRLGIPLIFGMDVVHGYETIFPIPLGLSCSWDMEAIRKSARVAAIEASADGISWTFSPMVDISRDPRWGRVSEGNGEDPFLGGAIAKAMVSGYQGIDLNNQLKRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEYMYPYQAAVDAGVGSVMASFNEIDGIPATANKWLMTDVLRKQWGFDGFVVTDFTGISEMIAHGIGDLQTVSARALNAGVDMDMVSEGFTGTIKKSIDEGKISMETLDKACRRILEAKYKLGLFDNPYKYCDLKRPKRDIFTKEHRDAARKIAGESFVLLKNDKSGSSANPTLPLKKEGTVAVIGPLANTRSNMPGTWSVAARLNDYPSVYEGLKEMMKGKVNITYAKGSNLISDAAYEERATMFGRSLNRDNRTDKEMLDEALKVAANADVIIAALGESSEMSGESSSRTNLALPDVQRTLLEALLKTGKPVVLTLFTGRPLTLTWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLLEGKWFEKFRSNYLDVDNDPLYPFGYGLSYTNFQYSDITLSAPTMGQDGSVTAMVTVTNTGKYDGAEVVQLYIRDLVGSITRPVKELKGFDKIFLKAGESKTVSFKITPELLRFYDYELNYVAEPGDFDIMIGGNSQSVKTTHLSL
AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTTTCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAAGGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCATGGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATCGAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAACGGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAACGATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAACCGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGCATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGTATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGTGAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAAGCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGCGACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAAAAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGACTATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCAGCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,682 total letters Query= APC115045.105 Length=2646 Score E Sequences producing significant alignments: (Bits) Value wt 4033 0.0 03D_D3_APC115045.105_D03.seq 1157 0.0 K S P Q D M D R F I D A L M K K M T V E E K I G Q L N L P V Query_1 202 AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTT 291 2 1 .......................................................................................... 90 S G E I V T G Q A Q N S D V A K K I E Q G L V G G L L N L K Query_1 292 TCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAA 381 2 91 .......................................................................................... 180 G V E K I R D V Q K L A I E K S R L G I P L I F G M D V V H Query_1 382 GGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCAT 471 2 181 .......................................................................................... 270 G Y E T I F P I P L G L S C S W D M E A I R K S A R V A A I Query_1 472 GGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATC 561 2 271 .......................................................................................... 360 E A S A D G I S W T F S P M V D I S R D P R W G R V S E G N Query_1 562 GAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAAC 651 2 361 .......................................................................................... 450 G E D P F L G G A I A K A M V S G Y Q G I D L N N Q L K R N Query_1 652 GGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAAC 741 2 451 .......................................................................................... 540 D E I M A C V K H F A L Y G A G E A G R D Y N T V D M S R N Query_1 742 GATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAAC 831 2 541 .......................................................................................... 630 R M F N E Y M Y P Y Q A A V D A G V G S V M A S F N E I D G Query_1 832 CGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGC 921 2 631 .......................................................................................... 720 I P A T A N K W L M T D V L R K Q W G F D G F V V T D F T G Query_1 922 ATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGT 1011 2 721 .......................................................................................... 810 I S E M I A H G I G D L Q T V S A R A L N A G V D M D M V S Query_1 1012 ATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGT 1101 2 811 .......................................................................................... 900 E G F T G T I K K S I D E G K I S M E T L D K A C R R I L E Query_1 1102 GAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAA 1191 2 901 .......................................................................................... 990 A K Y K L G L F D N P Y K Y C D L K R P K R D I F T K E H R Query_1 1192 GCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGC 1281 2 991 .......................................................................................... 1080 D A A R K I A G E S F V L L K N D K S G S S A N P T L P L K Query_1 1282 GACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAA 1371 2 1081 .......................................................................................... 1170 K E G T V A V I G P L A N T R S N M P G T W S V A A R L N D Query_1 1372 AAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGAC 1461 2 1171 .......................................................................................... 1260 Y P S V Y E G L K E M M K G K V N I T Y A K G S N L I S D A Query_1 1462 TATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCA 1551 2 1261 .......................................................................................... 1350 A Y E E R A T M F G R S L N R D N R T D K E M L D E A L K Query_1 1552 GCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTG-AAAGT 1640 2 1351 ....................................................................................-..... 1439 0 14 .......N..........T.N... 37 V A A N A D V I I A A L G E S S E M S G A S S S R T N L A L Query_1 1641 GGCCGCTAATGCAGATGTAATAATA-GCCGCATTGGGAGAATCATCTGAAATGAGTGGTGCTAGTTCAAGCCGCACTAACCTGGCTCTTC 1729 2 1440 .........................-..................................AA............................ 1528 0 38 .........................N................................................................ 127 P D V Q R T L L E A L L K T G K P V V L T L F T G R P L T L Query_1 1730 CCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGA 1819 2 1529 .......................................................................................... 1618 0 128 .......................................................................................... 217 T W E Q E H V P A I L N V W F G G S E A A Y A I G D V L F G Query_1 1820 CTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCG 1909 2 1619 .......................................................................................... 1708 0 218 .......................................................................................... 307 D V N P S G K L T M T F P K N V G Q I P L F Y N H K N T G R Query_1 1910 ATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTC 1999 2 1709 .......................................................................................... 1798 0 308 ...............................................N.......................................... 397 P L L E G K W F E K F R S N Y L D V D N D P L Y P F G Y G L Query_1 2000 CTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGT 2089 2 1799 .......................................................................................... 1888 0 398 ....................................N...................N................................. 487 S Y T N F Q Y S D I T L S A P T M G Q D G S V T A M V T V T Query_1 2090 CGTATA-CCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACC 2178 2 1889 ......-................................................................................... 1977 0 488 ......N....................................................N...........N..........N....... 577 N T G K Y D G A E V V Q L Y I R D L V G S I T R P V K E L Query_1 2179 AATACCGGTAAGTAC-GATGGTGCAGAAGTAGTGCAACTTTATAT-CCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTG- 2265 2 1978 ...............-.............................-...........................................- 2064 0 578 ...............N.........N..N................N..............................N.N....N.....A 667 K G F D K I F L K A G E S K T V S F K I T P E L L R F Query_1 2266 AAAGGGTTTGATAAAA-TTTTCCTCAAAGCGGGTGAAAG-TAAG-ACTGTATC-TTTCAAAATCACTCC-GGAATTACT-GCGC--TTCT 2347 2 2065 ................-......................-....-........-...............-.........-....--.... 2146 0 668 ........CN......T........NN.N...N.....NT....A.NN.NT..NN............N.G.........N..N.CT.N.. 757 Y D Y E L N Y V A E P G D F D I M I G G N S Q S V K Query_1 2348 ACGACTATGAAC-TCAACTA-CGTAG-CCGAA-CCGGG-AG-ACTT-CGACATAATGAT---CGGGGGGAA--CAGCCAAAGTGTAAAAA 2425 2 2147 ............-.......-.....-.....-.....-..-....-............---.........--................. 2224 0 758 N.N.........T..N.A..C.T...N.....N.....N..A....N.AN.........NCG......N..NCNNC....N....N.N.. 847 T T H L S L Query_1 2426 --CGACTCATCTGAGTTTG 2442 2 2225 --................. 2241 0 848 TC....N... 857 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 9545899 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 3,682 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115045.106 (APC115045.26-772.P(773).W437A.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVSGEIVTGQAQNSDVAKKIEQGLVGGLLNLKGVEKIRDVQKLAIEKSRLGIPLIFGMDVVHGYETIFPIPLGLSCSWDMEAIRKSARVAAIEASADGISWTFSPMVDISRDPRWGRVSEGNGEDPFLGGAIAKAMVSGYQGIDLNNQLKRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEYMYPYQAAVDAGVGSVMASFNEIDGIPATANKWLMTDVLRKQWGFDGFVVTDFTGISEMIAHGIGDLQTVSARALNAGVDMDMVSEGFTGTIKKSIDEGKISMETLDKACRRILEAKYKLGLFDNPYKYCDLKRPKRDIFTKEHRDAARKIAGESFVLLKNDKSGSSANPTLPLKKEGTVAVIGPLANTRSNMPGTWSVAARLNDYPSVYEGLKEMMKGKVNITYAKGSNLISDAAYEERATMFGRSLNRDNRTDKEMLDEALKVAANADVIIAALGESSEMSGESSSRTNLALPDVQRTLLEALLKTGKPVVLTLFTGRPLTLTWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLLEGKWFEKFRSNYLDVDNDPLYPFGYGLSYTNFQYSDITLSAPTMGQDGSVTAMVTVTNTGKYDGAEVVQLYIRDLVGSITRPVKELKGFDKIFLKAGESKTVSFKITPELLRFYDYELNYVAEPGDFDIMIGGNSQSVKTTHLSL
AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTTTCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAAGGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCATGGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATCGAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAACGGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAACGATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAACCGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGCATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGTATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGTGAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAAGCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGCGACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAAAAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGACTATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCAGCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,652 total letters Query= APC115045.106 Length=2646 Score E Sequences producing significant alignments: (Bits) Value wt 4028 0.0 03E_E3_APC115045.106_E03.seq 1251 0.0 K S P Q D M D R F I D A L M K K M T V E E K I G Q L N L P V Query_1 202 AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTT 291 2 1 .......................................................................................... 90 S G E I V T G Q A Q N S D V A K K I E Q G L V G G L L N L K Query_1 292 TCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAA 381 2 91 .......................................................................................... 180 G V E K I R D V Q K L A I E K S R L G I P L I F G M D V V H Query_1 382 GGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCAT 471 2 181 .......................................................................................... 270 G Y E T I F P I P L G L S C S W D M E A I R K S A R V A A I Query_1 472 GGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATC 561 2 271 .......................................................................................... 360 E A S A D G I S W T F S P M V D I S R D P R W G R V S E G N Query_1 562 GAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAAC 651 2 361 .......................................................................................... 450 G E D P F L G G A I A K A M V S G Y Q G I D L N N Q L K R N Query_1 652 GGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAAC 741 2 451 .......................................................................................... 540 D E I M A C V K H F A L Y G A G E A G R D Y N T V D M S R N Query_1 742 GATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAAC 831 2 541 .......................................................................................... 630 R M F N E Y M Y P Y Q A A V D A G V G S V M A S F N E I D G Query_1 832 CGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGC 921 2 631 .......................................................................................... 720 I P A T A N K W L M T D V L R K Q W G F D G F V V T D F T G Query_1 922 ATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGT 1011 2 721 .......................................................................................... 810 I S E M I A H G I G D L Q T V S A R A L N A G V D M D M V S Query_1 1012 ATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGT 1101 2 811 .......................................................................................... 900 E G F T G T I K K S I D E G K I S M E T L D K A C R R I L E Query_1 1102 GAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAA 1191 2 901 .......................................................................................... 990 A K Y K L G L F D N P Y K Y C D L K R P K R D I F T K E H R Query_1 1192 GCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGC 1281 2 991 .......................................................................................... 1080 D A A R K I A G E S F V L L K N D K S G S S A N P T L P L Query_1 1282 GACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGG-TTCCTCTGCAAACCCAACACTTCCTTTGAA 1370 2 1081 ...........................................................-.............................. 1169 0 7 .....NN..........CGC..........C..........N................... 67 K K E G T V A V I G P L A N T R S N M P G T A S V A A R L N Query_1 1371 AAAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAG-TAACATGCCGGGTACCGCTAGTGTAGCCGCACGCCTCAACG 1459 2 1170 ................................................-................TGG...................... 1258 0 68 ................................................N......................................... 157 D Y P S V Y E G L K E M M K G K V N I T Y A K G S N L I S D Query_1 1460 ACTATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATG 1549 2 1259 .......................................................................................... 1348 0 158 .......................................................................................... 247 A A Y E E R A T M F G R S L N R D N R T D K E M L D E A L K Query_1 1550 CAGCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAG 1639 2 1349 .......................................................................................... 1438 0 248 .......................................................................................... 337 V A A N A D V I I A A L G E S S E M S G E S S S R T N L A L Query_1 1640 TGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTC 1729 2 1439 .......................................................................................... 1528 0 338 .......................................................................................... 427 P D V Q R T L L E A L L K T G K P V V L T L F T G R P L T L Query_1 1730 CCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGA 1819 2 1529 .......................................................................................... 1618 0 428 .......................................................................................... 517 T W E Q E H V P A I L N V W F G G S E A A Y A I G D V L F G Query_1 1820 CTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTT-CGGC 1908 2 1619 .....................................................................................-.... 1707 0 518 ....N................................................................................C...T 607 D V N P S G K L T M T F P K N V G Q I P L F Y N H K N T Query_1 1909 GATGTAAAT-CCCAGCGGCAAACTAACG-ATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTT-TACAATCATAAAAATACC-GG 1994 2 1708 .........-..................-.......................................-..................-.. 1793 0 608 ......N..C..................A.....................T.................N...-...........N..G.. 696 G R P L L E G K W F E K F R S N Y L D V D N D P L Y P Query_1 1995 TCG-TCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCC--GTAGT--AATTACCTGGATGTAGACAACGATCC-ATTG--TATCCA-TT 2075 2 1794 ...-...................................--.....--..........................-....--......-.. 1874 0 697 ...T......N............................CGNN...NN.T.............N.....N....A....GNN.....T.. 786 F G Y G L S Y T N F Q Y S D I T L S A P T M G Q D G S Query_1 2076 CGGCTATGG-TTTGTCGTATA-CCAACTTTC-AATACA-GCGACATAA-CTCTGAGCGCCCC--GACT-ATGGGACAG-GATGGTTCTGT 2156 2 1875 .........-...........-.........-......-.........-.............--....-.........-........... 1955 0 787 ....C....T.......N...C..N.N....T.T.N..N.N.....N.C......N.N....CNA...A.N..T....N....... 872 V T A M V T V T N T G K Y D G A E V V Q L Y I R D L V G S I Query_1 2157 TACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCAC 2246 2 1956 .......................................................................................... 2045 T R P V K E L K G F D K I F L K A G E S K T V S F K I T P E Query_1 2247 CCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATT 2336 2 2046 .......................................................................................... 2135 L L R F Y D Y E L N Y V A E P G D F D I M I G G N S Q S V K Query_1 2337 ACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAAC 2426 2 2136 .......................................................................................... 2225 T T H L S L Query_1 2427 GACTCATCTGAGTTTG 2442 2 2226 ................ 2241 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 9467029 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 3,652 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115045.107 (APC115045.26-772.P(773).D112A.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVSGEIVTGQAQNSDVAKKIEQGLVGGLLNLKGVEKIRDVQKLAIEKSRLGIPLIFGMDVVHGYETIFPIPLGLSCSWDMEAIRKSARVAAIEASADGISWTFSPMVDISRDPRWGRVSEGNGEDPFLGGAIAKAMVSGYQGIDLNNQLKRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEYMYPYQAAVDAGVGSVMASFNEIDGIPATANKWLMTDVLRKQWGFDGFVVTDFTGISEMIAHGIGDLQTVSARALNAGVDMDMVSEGFTGTIKKSIDEGKISMETLDKACRRILEAKYKLGLFDNPYKYCDLKRPKRDIFTKEHRDAARKIAGESFVLLKNDKSGSSANPTLPLKKEGTVAVIGPLANTRSNMPGTWSVAARLNDYPSVYEGLKEMMKGKVNITYAKGSNLISDAAYEERATMFGRSLNRDNRTDKEMLDEALKVAANADVIIAALGESSEMSGESSSRTNLALPDVQRTLLEALLKTGKPVVLTLFTGRPLTLTWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLLEGKWFEKFRSNYLDVDNDPLYPFGYGLSYTNFQYSDITLSAPTMGQDGSVTAMVTVTNTGKYDGAEVVQLYIRDLVGSITRPVKELKGFDKIFLKAGESKTVSFKITPELLRFYDYELNYVAEPGDFDIMIGGNSQSVKTTHLSL
AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTTTCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAAGGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCATGGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATCGAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAACGGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAACGATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAACCGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGCATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGTATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGTGAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAAGCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGCGACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAAAAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGACTATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCAGCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,251 total letters Query= APC115045.107 Length=2646 Score E Sequences producing significant alignments: (Bits) Value wt 4033 0.0 K S P Q D M D R F I D A L M K K M T V E E K I G Q L N L P V Query_1 202 AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTT 291 2 1 .......................................................................................... 90 S G E I V T G Q A Q N S D V A K K I E Q G L V G G L L N L K Query_1 292 TCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAA 381 2 91 .......................................................................................... 180 G V E K I R D V Q K L A I E K S R L G I P L I F G M A V V H Query_1 382 GGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGCTGTAGTGCAT 471 2 181 ...............................................................................AC......... 270 G Y E T I F P I P L G L S C S W D M E A I R K S A R V A A I Query_1 472 GGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATC 561 2 271 .......................................................................................... 360 E A S A D G I S W T F S P M V D I S R D P R W G R V S E G N Query_1 562 GAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAAC 651 2 361 .......................................................................................... 450 G E D P F L G G A I A K A M V S G Y Q G I D L N N Q L K R N Query_1 652 GGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAAC 741 2 451 .......................................................................................... 540 D E I M A C V K H F A L Y G A G E A G R D Y N T V D M S R N Query_1 742 GATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAAC 831 2 541 .......................................................................................... 630 R M F N E Y M Y P Y Q A A V D A G V G S V M A S F N E I D G Query_1 832 CGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGC 921 2 631 .......................................................................................... 720 I P A T A N K W L M T D V L R K Q W G F D G F V V T D F T G Query_1 922 ATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGT 1011 2 721 .......................................................................................... 810 I S E M I A H G I G D L Q T V S A R A L N A G V D M D M V S Query_1 1012 ATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGT 1101 2 811 .......................................................................................... 900 E G F T G T I K K S I D E G K I S M E T L D K A C R R I L E Query_1 1102 GAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAA 1191 2 901 .......................................................................................... 990 A K Y K L G L F D N P Y K Y C D L K R P K R D I F T K E H R Query_1 1192 GCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGC 1281 2 991 .......................................................................................... 1080 D A A R K I A G E S F V L L K N D K S G S S A N P T L P L K Query_1 1282 GACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAA 1371 2 1081 .......................................................................................... 1170 K E G T V A V I G P L A N T R S N M P G T W S V A A R L N D Query_1 1372 AAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGAC 1461 2 1171 .......................................................................................... 1260 Y P S V Y E G L K E M M K G K V N I T Y A K G S N L I S D A Query_1 1462 TATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCA 1551 2 1261 .......................................................................................... 1350 A Y E E R A T M F G R S L N R D N R T D K E M L D E A L K V Query_1 1552 GCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTG 1641 2 1351 .......................................................................................... 1440 A A N A D V I I A A L G E S S E M S G E S S S R T N L A L P Query_1 1642 GCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCC 1731 2 1441 .......................................................................................... 1530 D V Q R T L L E A L L K T G K P V V L T L F T G R P L T L T Query_1 1732 GATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACT 1821 2 1531 .......................................................................................... 1620 W E Q E H V P A I L N V W F G G S E A A Y A I G D V L F G D Query_1 1822 TGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGAT 1911 2 1621 .......................................................................................... 1710 V N P S G K L T M T F P K N V G Q I P L F Y N H K N T G R P Query_1 1912 GTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCT 2001 2 1711 .......................................................................................... 1800 L L E G K W F E K F R S N Y L D V D N D P L Y P F G Y G L S Query_1 2002 TTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCG 2091 2 1801 .......................................................................................... 1890 Y T N F Q Y S D I T L S A P T M G Q D G S V T A M V T V T N Query_1 2092 TATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAAT 2181 2 1891 .......................................................................................... 1980 T G K Y D G A E V V Q L Y I R D L V G S I T R P V K E L K G Query_1 2182 ACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGG 2271 2 1981 .......................................................................................... 2070 F D K I F L K A G E S K T V S F K I T P E L L R F Y D Y E L Query_1 2272 TTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTC 2361 2 2071 .......................................................................................... 2160 N Y V A E P G D F D I M I G G N S Q S V K T T H L S L Query_1 2362 AACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG 2442 2 2161 ................................................................................. 2241 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5793890 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 2,251 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115045.110 (APC115045.26-772.P(773).K213A.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVSGEIVTGQAQNSDVAKKIEQGLVGGLLNLKGVEKIRDVQKLAIEKSRLGIPLIFGMDVVHGYETIFPIPLGLSCSWDMEAIRKSARVAAIEASADGISWTFSPMVDISRDPRWGRVSEGNGEDPFLGGAIAKAMVSGYQGIDLNNQLKRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEYMYPYQAAVDAGVGSVMASFNEIDGIPATANKWLMTDVLRKQWGFDGFVVTDFTGISEMIAHGIGDLQTVSARALNAGVDMDMVSEGFTGTIKKSIDEGKISMETLDKACRRILEAKYKLGLFDNPYKYCDLKRPKRDIFTKEHRDAARKIAGESFVLLKNDKSGSSANPTLPLKKEGTVAVIGPLANTRSNMPGTWSVAARLNDYPSVYEGLKEMMKGKVNITYAKGSNLISDAAYEERATMFGRSLNRDNRTDKEMLDEALKVAANADVIIAALGESSEMSGESSSRTNLALPDVQRTLLEALLKTGKPVVLTLFTGRPLTLTWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLLEGKWFEKFRSNYLDVDNDPLYPFGYGLSYTNFQYSDITLSAPTMGQDGSVTAMVTVTNTGKYDGAEVVQLYIRDLVGSITRPVKELKGFDKIFLKAGESKTVSFKITPELLRFYDYELNYVAEPGDFDIMIGGNSQSVKTTHLSL
AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTTTCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAAGGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCATGGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATCGAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAACGGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAACGATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAACCGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGCATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGTATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGTGAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAAGCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGCGACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAAAAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGACTATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCAGCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,626 total letters Query= APC115045.110 Length=2646 Score E Sequences producing significant alignments: (Bits) Value wt 4028 0.0 03G_G3_APC115045.110_G03.seq 1209 0.0 K S P Q D M D R F I D A L M K K M T V E E K I G Q L N L P V Query_1 202 AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTT 291 2 1 .......................................................................................... 90 S G E I V T G Q A Q N S D V A K K I E Q G L V G G L L N L K Query_1 292 TCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAA 381 2 91 .......................................................................................... 180 G V E K I R D V Q K L A I E K S R L G I P L I F G M D V V H Query_1 382 GGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCAT 471 2 181 .......................................................................................... 270 G Y E T I F P I P L G L S C S W D M E A I R K S A R V A A I Query_1 472 GGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATC 561 2 271 .......................................................................................... 360 E A S A D G I S W T F S P M V D I S R D P R W G R V S E G N Query_1 562 GAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAAC 651 2 361 .......................................................................................... 450 G E D P F L G G A I A K A M V S G Y Q G I D L N N Q L K R N Query_1 652 GGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAAC 741 2 451 .......................................................................................... 540 0 3 ....N.N....................................................NT 63 D E I M A C V A H F A L Y G A G E A G R D Y N T V D M S R Query_1 742 G-ATGAAATTATGGCATGTGTAGCTCACTTCGCACTG-TATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTA 829 2 541 .-....................AAG............-.................................................... 628 0 64 TT...................................C.................................................... 153 N R M F N E Y M Y P Y Q A A V D A G V G S V M A S F N E I D Query_1 830 ACCGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACG 919 2 629 .......................................................................................... 718 0 154 .......................................................................................... 243 G I P A T A N K W L M T D V L R K Q W G F D G F V V T D F T Query_1 920 GCATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCG 1009 2 719 .......................................................................................... 808 0 244 .......................................................................................... 333 G I S E M I A H G I G D L Q T V S A R A L N A G V D M D M V Query_1 1010 GTATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAA 1099 2 809 .......................................................................................... 898 0 334 ..................................................................N....................... 423 S E G F T G T I K K S I D E G K I S M E T L D K A C R R I L Query_1 1100 GTGAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTG 1189 2 899 .......................................................................................... 988 0 424 .......................................................................................... 513 E A K Y K L G L F D N P Y K Y C D L K R P K R D I F T K E H Query_1 1190 AAGCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATC 1279 2 989 .......................................................................................... 1078 0 514 ..................................N..............................................N........ 603 R D A A R K I A G E S F V L L K N D K S G S S A N P T L Query_1 1280 GCGACG-CTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACT-CCTGAAAAACGACAAG-TCAGGTTCCTCTGCAAA--CCCAACACTTCC 1364 2 1079 ......-..................................-................-.................--............ 1163 0 604 .....NC..................................C................A...N.NN..........AC..N.N....... 693 P L K K E G T V A V I G P L A N T R S N M P G T W S V A A Query_1 1365 TTTG-AAAAAAGAAGGTACGGTGGCTGTCATCGGCCC-ACTGGC-AAATA-CCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCAC 1450 2 1164 ....-................................-......-.....-....................................... 1249 0 694 ....A......N.N.......N...............N......A.....C.......... 754 R L N D Y P S V Y E G L K E M M K G K V N I T Y A K G S N L Query_1 1451 GCCTCAACGACTATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCA 1540 2 1250 .......................................................................................... 1339 I S D A A Y E E R A T M F G R S L N R D N R T D K E M L D E Query_1 1541 TCAGTGATGCAGCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGG 1630 2 1340 .......................................................................................... 1429 A L K V A A N A D V I I A A L G E S S E M S G E S S S R T N Query_1 1631 CGCTGAAAGTGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACC 1720 2 1430 .......................................................................................... 1519 L A L P D V Q R T L L E A L L K T G K P V V L T L F T G R P Query_1 1721 TGGCTCTTCCCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCAC 1810 2 1520 .......................................................................................... 1609 L T L T W E Q E H V P A I L N V W F G G S E A A Y A I G D V Query_1 1811 TAACGTTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTAT 1900 2 1610 .......................................................................................... 1699 L F G D V N P S G K L T M T F P K N V G Q I P L F Y N H K N Query_1 1901 TGTTCGGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATA 1990 2 1700 .......................................................................................... 1789 T G R P L L E G K W F E K F R S N Y L D V D N D P L Y P F G Query_1 1991 CCGGTCGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCT 2080 2 1790 .......................................................................................... 1879 Y G L S Y T N F Q Y S D I T L S A P T M G Q D G S V T A M V Query_1 2081 ATGGTTTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCA 2170 2 1880 .......................................................................................... 1969 0 97 ........... 107 T V T N T G K Y D G A E V V Q L Y I R D L V G S I T R P V K Query_1 2171 CGGTAACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAG 2260 2 1970 .......................................................................................... 2059 E L K G F D K I F L K A G E S K T V S F K I T P E L L R F Y Query_1 2261 AACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACG 2350 2 2060 .......................................................................................... 2149 D Y E L N Y V A E P G D F D I M I G G N S Q S V K T T H L S Query_1 2351 ACTATGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTT 2440 2 2150 .......................................................................................... 2239 L Query_1 2441 TG 2442 2 2240 .. 2241 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 9398675 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 3,626 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115045.111 (APC115045.26-772.P(773).H214A.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVSGEIVTGQAQNSDVAKKIEQGLVGGLLNLKGVEKIRDVQKLAIEKSRLGIPLIFGMDVVHGYETIFPIPLGLSCSWDMEAIRKSARVAAIEASADGISWTFSPMVDISRDPRWGRVSEGNGEDPFLGGAIAKAMVSGYQGIDLNNQLKRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEYMYPYQAAVDAGVGSVMASFNEIDGIPATANKWLMTDVLRKQWGFDGFVVTDFTGISEMIAHGIGDLQTVSARALNAGVDMDMVSEGFTGTIKKSIDEGKISMETLDKACRRILEAKYKLGLFDNPYKYCDLKRPKRDIFTKEHRDAARKIAGESFVLLKNDKSGSSANPTLPLKKEGTVAVIGPLANTRSNMPGTWSVAARLNDYPSVYEGLKEMMKGKVNITYAKGSNLISDAAYEERATMFGRSLNRDNRTDKEMLDEALKVAANADVIIAALGESSEMSGESSSRTNLALPDVQRTLLEALLKTGKPVVLTLFTGRPLTLTWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLLEGKWFEKFRSNYLDVDNDPLYPFGYGLSYTNFQYSDITLSAPTMGQDGSVTAMVTVTNTGKYDGAEVVQLYIRDLVGSITRPVKELKGFDKIFLKAGESKTVSFKITPELLRFYDYELNYVAEPGDFDIMIGGNSQSVKTTHLSL
AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTTTCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAAGGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCATGGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATCGAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAACGGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAACGATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAACCGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGCATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGTATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGTGAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAAGCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGCGACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAAAAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGACTATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCAGCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,251 total letters Query= APC115045.111 Length=2646 Score E Sequences producing significant alignments: (Bits) Value wt 4028 0.0 K S P Q D M D R F I D A L M K K M T V E E K I G Q L N L P V Query_1 202 AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTT 291 2 1 .......................................................................................... 90 S G E I V T G Q A Q N S D V A K K I E Q G L V G G L L N L K Query_1 292 TCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAA 381 2 91 .......................................................................................... 180 G V E K I R D V Q K L A I E K S R L G I P L I F G M D V V H Query_1 382 GGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCAT 471 2 181 .......................................................................................... 270 G Y E T I F P I P L G L S C S W D M E A I R K S A R V A A I Query_1 472 GGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATC 561 2 271 .......................................................................................... 360 E A S A D G I S W T F S P M V D I S R D P R W G R V S E G N Query_1 562 GAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAAC 651 2 361 .......................................................................................... 450 G E D P F L G G A I A K A M V S G Y Q G I D L N N Q L K R N Query_1 652 GGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAAC 741 2 451 .......................................................................................... 540 D E I M A C V K A F A L Y G A G E A G R D Y N T V D M S R N Query_1 742 GATGAAATTATGGCATGTGTAAAGGCTTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAAC 831 2 541 ........................CAC............................................................... 630 R M F N E Y M Y P Y Q A A V D A G V G S V M A S F N E I D G Query_1 832 CGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGC 921 2 631 .......................................................................................... 720 I P A T A N K W L M T D V L R K Q W G F D G F V V T D F T G Query_1 922 ATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGT 1011 2 721 .......................................................................................... 810 I S E M I A H G I G D L Q T V S A R A L N A G V D M D M V S Query_1 1012 ATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGT 1101 2 811 .......................................................................................... 900 E G F T G T I K K S I D E G K I S M E T L D K A C R R I L E Query_1 1102 GAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAA 1191 2 901 .......................................................................................... 990 A K Y K L G L F D N P Y K Y C D L K R P K R D I F T K E H R Query_1 1192 GCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGC 1281 2 991 .......................................................................................... 1080 D A A R K I A G E S F V L L K N D K S G S S A N P T L P L K Query_1 1282 GACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAA 1371 2 1081 .......................................................................................... 1170 K E G T V A V I G P L A N T R S N M P G T W S V A A R L N D Query_1 1372 AAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGAC 1461 2 1171 .......................................................................................... 1260 Y P S V Y E G L K E M M K G K V N I T Y A K G S N L I S D A Query_1 1462 TATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCA 1551 2 1261 .......................................................................................... 1350 A Y E E R A T M F G R S L N R D N R T D K E M L D E A L K V Query_1 1552 GCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTG 1641 2 1351 .......................................................................................... 1440 A A N A D V I I A A L G E S S E M S G E S S S R T N L A L P Query_1 1642 GCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCC 1731 2 1441 .......................................................................................... 1530 D V Q R T L L E A L L K T G K P V V L T L F T G R P L T L T Query_1 1732 GATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACT 1821 2 1531 .......................................................................................... 1620 W E Q E H V P A I L N V W F G G S E A A Y A I G D V L F G D Query_1 1822 TGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGAT 1911 2 1621 .......................................................................................... 1710 V N P S G K L T M T F P K N V G Q I P L F Y N H K N T G R P Query_1 1912 GTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCT 2001 2 1711 .......................................................................................... 1800 L L E G K W F E K F R S N Y L D V D N D P L Y P F G Y G L S Query_1 2002 TTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCG 2091 2 1801 .......................................................................................... 1890 Y T N F Q Y S D I T L S A P T M G Q D G S V T A M V T V T N Query_1 2092 TATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAAT 2181 2 1891 .......................................................................................... 1980 T G K Y D G A E V V Q L Y I R D L V G S I T R P V K E L K G Query_1 2182 ACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGG 2271 2 1981 .......................................................................................... 2070 F D K I F L K A G E S K T V S F K I T P E L L R F Y D Y E L Query_1 2272 TTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTC 2361 2 2071 .......................................................................................... 2160 N Y V A E P G D F D I M I G G N S Q S V K T T H L S L Query_1 2362 AACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG 2442 2 2161 ................................................................................. 2241 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5793890 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 2,251 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115045.112 (APC115045.26-772.P(773).F260A.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVSGEIVTGQAQNSDVAKKIEQGLVGGLLNLKGVEKIRDVQKLAIEKSRLGIPLIFGMDVVHGYETIFPIPLGLSCSWDMEAIRKSARVAAIEASADGISWTFSPMVDISRDPRWGRVSEGNGEDPFLGGAIAKAMVSGYQGIDLNNQLKRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEYMYPYQAAVDAGVGSVMASFNEIDGIPATANKWLMTDVLRKQWGFDGFVVTDFTGISEMIAHGIGDLQTVSARALNAGVDMDMVSEGFTGTIKKSIDEGKISMETLDKACRRILEAKYKLGLFDNPYKYCDLKRPKRDIFTKEHRDAARKIAGESFVLLKNDKSGSSANPTLPLKKEGTVAVIGPLANTRSNMPGTWSVAARLNDYPSVYEGLKEMMKGKVNITYAKGSNLISDAAYEERATMFGRSLNRDNRTDKEMLDEALKVAANADVIIAALGESSEMSGESSSRTNLALPDVQRTLLEALLKTGKPVVLTLFTGRPLTLTWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLLEGKWFEKFRSNYLDVDNDPLYPFGYGLSYTNFQYSDITLSAPTMGQDGSVTAMVTVTNTGKYDGAEVVQLYIRDLVGSITRPVKELKGFDKIFLKAGESKTVSFKITPELLRFYDYELNYVAEPGDFDIMIGGNSQSVKTTHLSL
AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTTTCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAAGGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCATGGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATCGAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAACGGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAACGATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAACCGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGCATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGTATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGTGAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAAGCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGCGACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAAAAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGACTATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCAGCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,869 total letters Query= APC115045.112 Length=2646 Score E Sequences producing significant alignments: (Bits) Value wt 4028 0.0 10A_A4_APC115045.112_A04.seq 1719 0.0 04A_A4_APC115045.112_A04.seq 1389 0.0 R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L Query_1 3 TCGATCCCGCGAAATTAATA-CGACTCACTATAGG-GGAATTGTGAGC--GGATAACAATT-CCCCTCTAGAAATAATTTTGTTTAACTT 87 1 1124 .....T..CN.N...N....TN.......CN....N..........ANAG.....CN....T....NN.NNG....-.......N.G... 1036 * E G D I H M H H H H H H S S G V D L W S H P Q F E K G T Query_1 88 TAAGAAGGA-GATATACATATGCACC-ATCATCATCATCATTCTTCTGGTGTA-GATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACC 174 1 1035 N.GN..A..A..N...-...N..N..C...............NN.NCN.G...ANN......A.N.....N......N..........N. 947 E N L Y F Q S N A K S P Q D M D R F I D A L M K K M T V E Query_1 175 GAGAACCTGTACTTCCAATCCAATGCCAAGTCTCC-GCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAA-GATGACTGTAGAGG 262 2 1 ........-.......................................-.............. 61 1 946 ...N.............T...............N.N.............N.................N.......A.............. 857 E K I G Q L N L P V S G E I V T G Q A Q N S D V A K K I E Q Query_1 263 AAAAGATCGGTCAGCTGAACCTACCCGTTTCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAG 352 2 62 .......................................................................................... 151 1 856 .........-..................................................-............................. 769 G L V G G L L N L K G V E K I R D V Q K L A I E K S R L G I Query_1 353 GGCTCGTGGGCGGACTCCTCAACCTGAAAGGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCC 442 2 152 .......................................................................................... 241 1 768 .......................................................................................... 679 P L I F G M D V V H G Y E T I F P I P L G L S C S W D M E A Query_1 443 CCCTGATATTCGGCATGGACGTAGTGCATGGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCA 532 2 242 .......................................................................................... 331 1 678 .......................................................................................... 589 I R K S A R V A A I E A S A D G I S W T F S P M V D I S R D Query_1 533 TCAGGAAATCTGCCCGCGTTGCAGCCATCGAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATC 622 2 332 .......................................................................................... 421 1 588 .......................................................................................... 499 P R W G R V S E G N G E D P F L G G A I A K A M V S G Y Q G Query_1 623 CGCGCTGGGGACGCGTCAGCGAGGGTAACGGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTA 712 2 422 .......................................................................................... 511 1 498 .......................................................................................... 409 0 10 ...................... 31 I D L N N Q L K R N D E I M A C V K H F A L Y G A G E A G R Query_1 713 TCGACCTCAACAACCAACTGAAGCGCAACGATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTG 802 2 512 .......................................................................................... 601 1 408 .......................................................................................... 319 0 32 .......................................................................................... 121 D Y N T V D M S R N R M F N E Y M Y P Y Q A A V D A G V G S Query_1 803 ATTACAATACCGTAGATATGAGTCGTAACCGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCG 892 2 602 .......................................................................................... 691 1 318 .......................................................................................... 229 0 122 .......................................................................................... 211 V M A S A N E I D G I P A T A N K W L M T D V L R K Q W G F Query_1 893 TAATGGCGTCTGCTAACGAAATAGACGGCATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCG 982 2 692 ...........TTC............................................................................ 781 1 228 .......................................................................................... 139 0 212 .......................................................................................... 301 D G F V V T D F T G I S E M I A H G I G D L Q T V S A R A L Query_1 983 ACGGCTTTGTGGTGACAGACTTTACCGGTATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCA 1072 2 782 .......................................................................................... 871 1 138 .......................................................................................... 49 0 302 .......................................................................................... 391 N A G V D M D M V S E G F T G T I K K S I D E G K I S M E T Query_1 1073 ATGCAGGCGTGGATATGGACATGGTAAGTGAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCC 1162 2 872 .......................................................................................... 961 1 48 ............................ 21 0 392 .......................................................................................... 481 L D K A C R R I L E A K Y K L G L F D N P Y K Y C D L K R P Query_1 1163 TGGACAAAGCCTGTCGCCGCATCCTTGAAGCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGA 1252 2 962 .......................................................................................... 1051 0 482 .......................................................................................... 571 K R D I F T K E H R D A A R K I A G E S F V L L K N D K S G Query_1 1253 AGCGTGACATCTTCACCAAGGAACATCGCGACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTT 1342 2 1052 .......................................................................................... 1141 0 572 .......................................................................................... 661 S S A N P T L P L K K E G T V A V I G P L A N T R S N M Query_1 1343 CCTCTGCAAACCCAACACTTCCTTTGAAAAAAGAAGG-TACGGTGGCTGTCATCGGCCCA-CTGGC-AAATACCCGCAGTAACATGCC-- 1427 2 1142 .....................................-......................-.....-.....................-- 1226 0 662 .......................C.............T.N....................C.....A...........N....N..N.CG 751 P G T W S V A A R L N D Y P S V Y E G L K E M M K G K V N Query_1 1428 GGGTACCTGGAGTGTAGCCGCACGCCTCAACGACTA-TCCTTCTGTGTACGAAGGATTGAAAG-AGATGATGAAAGGCAAGG-TAAACAT 1514 2 1227 ....................................-..........................-..................-....... 1313 0 752 ......N................C.N.......N.NT.............N...........NA.N...N.N..N...C...N....N.. 841 I T Y A K G S N L I S D A A Y E E R A T M F G R S L N R D Query_1 1515 CAC-TTATG-CCAAAGGTAG-TAACCTCATCAGTGATGCAGCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAA 1601 2 1314 ...-.....-..........-..................................................................... 1400 0 842 .C.N.....N.....N..N.T....N..... 872 N R T D K E M L D E A L K V A A N A D V I I A A L G E S S E Query_1 1602 TCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAAT 1691 2 1401 .......................................................................................... 1490 M S G E S S S R T N L A L P D V Q R T L L E A L L K T G K P Query_1 1692 GAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGT 1781 2 1491 .......................................................................................... 1580 V V L T L F T G R P L T L T W E Q E H V P A I L N V W F G G Query_1 1782 TGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAG 1871 2 1581 .......................................................................................... 1670 S E A A Y A I G D V L F G D V N P S G K L T M T F P K N V G Query_1 1872 TGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCA 1961 2 1671 .......................................................................................... 1760 Q I P L F Y N H K N T G R P L L E G K W F E K F R S N Y L D Query_1 1962 AATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGT 2051 2 1761 .......................................................................................... 1850 V D N D P L Y P F G Y G L S Y T N F Q Y S D I T L S A P T M Query_1 2052 AGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGG 2141 2 1851 .......................................................................................... 1940 G Q D G S V T A M V T V T N T G K Y D G A E V V Q L Y I R D Query_1 2142 ACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCT 2231 2 1941 .......................................................................................... 2030 L V G S I T R P V K E L K G F D K I F L K A G E S K T V S F Query_1 2232 TGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAA 2321 2 2031 .......................................................................................... 2120 K I T P E L L R F Y D Y E L N Y V A E P G D F D I M I G G N Query_1 2322 AATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAG 2411 2 2121 .......................................................................................... 2210 S Q S V K T T H L S L Query_1 2412 CCAAAGTGTAAAAACGACTCATCTGAGTTTG 2442 2 2211 ............................... 2241 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12666522 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 4,869 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115045.113 (APC115045.26-772.P(773).F293A.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVSGEIVTGQAQNSDVAKKIEQGLVGGLLNLKGVEKIRDVQKLAIEKSRLGIPLIFGMDVVHGYETIFPIPLGLSCSWDMEAIRKSARVAAIEASADGISWTFSPMVDISRDPRWGRVSEGNGEDPFLGGAIAKAMVSGYQGIDLNNQLKRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEYMYPYQAAVDAGVGSVMASFNEIDGIPATANKWLMTDVLRKQWGFDGFVVTDFTGISEMIAHGIGDLQTVSARALNAGVDMDMVSEGFTGTIKKSIDEGKISMETLDKACRRILEAKYKLGLFDNPYKYCDLKRPKRDIFTKEHRDAARKIAGESFVLLKNDKSGSSANPTLPLKKEGTVAVIGPLANTRSNMPGTWSVAARLNDYPSVYEGLKEMMKGKVNITYAKGSNLISDAAYEERATMFGRSLNRDNRTDKEMLDEALKVAANADVIIAALGESSEMSGESSSRTNLALPDVQRTLLEALLKTGKPVVLTLFTGRPLTLTWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLLEGKWFEKFRSNYLDVDNDPLYPFGYGLSYTNFQYSDITLSAPTMGQDGSVTAMVTVTNTGKYDGAEVVQLYIRDLVGSITRPVKELKGFDKIFLKAGESKTVSFKITPELLRFYDYELNYVAEPGDFDIMIGGNSQSVKTTHLSL
AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTTTCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAAGGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCATGGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATCGAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAACGGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAACGATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAACCGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGCATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGTATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGTGAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAAGCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGCGACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAAAAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGACTATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCAGCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,994 total letters Query= APC115045.113 Length=2646 Score E Sequences producing significant alignments: (Bits) Value wt 4033 0.0 10B_B4_APC115045.113_B04.seq 1261 0.0 04B_B4_APC115045.113_B04.seq 1213 0.0 K G T E N L Y F Q S N A K S P Q D M D R F I D A L Query_1 164 AAAAGGG--TACCGAGAACC-TGTACTT-CCAATCC-AATGCCAAGTCTCCGCAGGA-CATGGAT--CGCTTCATCGATG-CATT-GA-T 242 2 1 ..............-.......--.............-....-..-. 41 1 981 .......GNN...NN..N..N.T.....N.....N.N.....N..T.T..G...N..G.....N.TCN.TN....TN...G....T..N. 892 M K K M T V E E K I G Q L N L P V S G E I V T G Q A Query_1 243 GAAGAAGATGACTGT-AGAGGAAAA-GAT-CGGT-CAGCT-GAACCT--ACCCG--TTTCC-GGCGAGATCGTCACCGGG--CAGGCA-C 319 2 42 ...............-.........-...-....-.....-......--.....--.....-..................--......-. 118 1 891 .....G...N...N.TN........A...N....T.....N.N...NTA....NGT.....N..N.....TN.....C..GC.N....A. 802 Q N S D V A K K I E Q G L V G G L L N L K G V E K I Query_1 320 AAAAC--AGCGATG-TGGC--AAAAAAGATTGAACAAGGG-CT-CGTGGGCGGACT-CCTCAA-CCTGAAA--GGGGTGGAG-AAGATA- 396 2 119 .....--.......-....--...................-..-............-......-.......--.........-......- 195 1 801 .....CAN......N.N..CA...N.N...GN........N..GNN........A.T......A.....N.AG.......N.N......N 712 R D V Q K L A I E K S R L G I P L I F G M D V V H G Y E T Query_1 397 CGCGATGTACAAAA--ACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCATGGTTACGAAACCA 484 2 196 ..............--.......................................................................... 283 1 711 ..........T...GA..................................N....................................... 622 I F P I P L G L S C S W D M E A I R K S A R V A A I E A S A Query_1 485 TTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATCGAGGCCAGTGCTG 574 2 284 .......................................................................................... 373 1 621 ....N.......................................N.......................................NA.... 532 D G I S W T F S P M V D I S R D P R W G R V S E G N G E D P Query_1 575 ATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAACGGCGAAGACCCAT 664 2 374 .......................................................................................... 463 1 531 .............................................................N............................ 442 F L G G A I A K A M V S G Y Q G I D L N N Q L K R N D E I Query_1 665 TCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCG-GGTTATCA-GGGTATCGACCTCAACAACCAACTGAAGCGCAACGATGAAATTAT 752 2 464 ...................................-........-............................................. 551 1 441 ...................................-........-............................................. 354 0 12 .....C........T.............N..................T............ 71 M A C V K H F A L Y G A G E A G R D Y N T V D M S R N R M F Query_1 753 GGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAACCGTATGTTCAA 842 2 552 .......................................................................................... 641 1 353 .......................................................................................... 264 0 72 .......................................................................................... 161 N E Y M Y P Y Q A A V D A G V G S V M A S F N E I D G I P A Query_1 843 CGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGCATACCAGCCAC 932 2 642 .......................................................................................... 731 1 263 .......................................................................................... 174 0 162 .......................................................................................... 251 T A N K W L M T D V L R K Q W G F D G F V V T D A T G I S E Query_1 933 GGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACGCTACCGGTATCTCCGAAAT 1022 2 732 ......................................................................TT.................. 821 1 173 .......................................................................................... 84 0 252 .......................................................................................... 341 M I A H G I G D L Q T V S A R A L N A G V D M D M V S E G Query_1 1023 GATAGCGCACGGCATCGGTG-ACTTGCAGA-CTGTTTCCG-CACGTGCACTCAATGCAGG-CGTGGATATGGA-CATGGTAAGTGAAGGC 1107 2 822 ....................-.........-.........-...................-............-................ 906 1 83 ....................A.N.......G.........A...................A............N..... 5 0 342 .....N..............-.........-.........-...................-............-................ 426 F T G T I K K S I D E G K I S M E T L D K A C R R I L E A K Query_1 1108 TTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAAGCCAAA 1197 2 907 .......................................................................................... 996 0 427 ...............N.......................................................................... 516 Y K L G L F D N P Y K Y C D L K R P K R D I F T K E H R D Query_1 1198 TACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACG-CCCGAAGCGTGACATCTTCACCAA-GGAACATCGCGACG 1285 2 997 ..................................................-........................-.............. 1084 0 517 .................................C................C........................G.....N...N.... 606 A A R K I A G E S F V L L K N D K S G S S A N P T L P L Query_1 1286 CTGCTCGTAAGATTGCGGGAGAG-AGC-TTTGTACTCCTGAAAAACGACAAGTCAGG-TTCCTC-TGC-AAACCCAACACTTCCTTTGAA 1370 2 1085 .......................-...-.............................-......-...-..................... 1169 0 607 .................N.....A...T.....N..................N..N.T......T...A..................... 696 K K E G T V A V I G P L A N T R S N M P G T W S V A A Query_1 1371 AAAA--GAAGGTACGGTGGCTGTCATC-GGCCCACTGGCA-AATACCCGCAGT-AACATG-CCGGGT-ACCTGG-AGTGT-AGCCGCACG 1451 2 1170 ....--.....................-............-............-......-......-......-.....-......... 1250 0 697 ....AGT..N......N..........G..TN.N......T............N.N....C......N......N.....N....N.N.. 786 R L N D Y P S V Y E G L K E M M K G K V N I T Y A K Query_1 1452 CC-TCAACGA-CTATCCTTC-TGTGTACG-AAGGATTG-AAAGA--GATGATGAAA-GGCAA-GGTAAA--CATCACTT-ATG-CCAAAG 1528 2 1251 ..-.......-.........-........-........-.....--..........-.....-......--........-...-...... 1327 0 787 ..T.....N.C..N..T...N...N.N.NA........N.....ACA..N......N.....N......ACNT......A...T.A.... 876 G S N L I S D A A Y E E R A T M F G R S L N R D N R T D Query_1 1529 GTA--GTAACCTCATCAGTGATGCAGCCTA--CGAAGAACG-TGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAA 1613 2 1328 ...--.........................--.........-................................................ 1412 0 877 ...NN.NNC.N.N..N.....N.NNAN..NTC.A.......T...TNN.......N... 935 K E M L D E A L K V A A N A D V I I A A L G E S S E M S G E Query_1 1614 AGAGATGCTGGATGAGGCGCTGAAAGTGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAG 1703 2 1413 .......................................................................................... 1502 S S S R T N L A L P D V Q R T L L E A L L K T G K P V V L T Query_1 1704 TTCAAGCCGCACTAACCTGGCTCTTCCCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCT 1793 2 1503 .......................................................................................... 1592 L F T G R P L T L T W E Q E H V P A I L N V W F G G S E A A Query_1 1794 CTTTACAGGTCGCCCACTAACGTTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATA 1883 2 1593 .......................................................................................... 1682 Y A I G D V L F G D V N P S G K L T M T F P K N V G Q I P L Query_1 1884 CGCCATTGGCGATGTATTGTTCGGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTT 1973 2 1683 .......................................................................................... 1772 F Y N H K N T G R P L L E G K W F E K F R S N Y L D V D N D Query_1 1974 TTACAATCATAAAAATACCGGTCGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCC 2063 2 1773 .......................................................................................... 1862 P L Y P F G Y G L S Y T N F Q Y S D I T L S A P T M G Q D G Query_1 2064 ATTGTATCCATTCGGCTATGGTTTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTC 2153 2 1863 .......................................................................................... 1952 S V T A M V T V T N T G K Y D G A E V V Q L Y I R D L V G S Query_1 2154 TGTTACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCAT 2243 2 1953 .......................................................................................... 2042 I T R P V K E L K G F D K I F L K A G E S K T V S F K I T P Query_1 2244 CACCCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGA 2333 2 2043 .......................................................................................... 2132 E L L R F Y D Y E L N Y V A E P G D F D I M I G G N S Q S V Query_1 2334 ATTACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAA 2423 2 2133 .......................................................................................... 2222 K T T H L S L Query_1 2424 AACGACTCATCTGAGTTTG 2442 2 2223 ................... 2241 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12995147 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 4,994 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115045.114 (APC115045.26-772.P(773).F635A.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVSGEIVTGQAQNSDVAKKIEQGLVGGLLNLKGVEKIRDVQKLAIEKSRLGIPLIFGMDVVHGYETIFPIPLGLSCSWDMEAIRKSARVAAIEASADGISWTFSPMVDISRDPRWGRVSEGNGEDPFLGGAIAKAMVSGYQGIDLNNQLKRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEYMYPYQAAVDAGVGSVMASFNEIDGIPATANKWLMTDVLRKQWGFDGFVVTDFTGISEMIAHGIGDLQTVSARALNAGVDMDMVSEGFTGTIKKSIDEGKISMETLDKACRRILEAKYKLGLFDNPYKYCDLKRPKRDIFTKEHRDAARKIAGESFVLLKNDKSGSSANPTLPLKKEGTVAVIGPLANTRSNMPGTWSVAARLNDYPSVYEGLKEMMKGKVNITYAKGSNLISDAAYEERATMFGRSLNRDNRTDKEMLDEALKVAANADVIIAALGESSEMSGESSSRTNLALPDVQRTLLEALLKTGKPVVLTLFTGRPLTLTWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLLEGKWFEKFRSNYLDVDNDPLYPFGYGLSYTNFQYSDITLSAPTMGQDGSVTAMVTVTNTGKYDGAEVVQLYIRDLVGSITRPVKELKGFDKIFLKAGESKTVSFKITPELLRFYDYELNYVAEPGDFDIMIGGNSQSVKTTHLSL
AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTTTCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAAGGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCATGGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATCGAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAACGGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAACGATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAACCGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGCATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGTATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGTGAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAAGCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGCGACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAAAAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGACTATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCAGCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,614 total letters Query= APC115045.114 Length=2646 Score E Sequences producing significant alignments: (Bits) Value wt 4028 0.0 10C_C4_APC115045.114_C04.seq 250 3e-69 K S P Q D M D R F I D A L M K K M T V E E K I G Q L N L P V Query_1 202 AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTT 291 2 1 .......................................................................................... 90 S G E I V T G Q A Q N S D V A K K I E Q G L V G G L L N L K Query_1 292 TCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAA 381 2 91 .......................................................................................... 180 G V E K I R D V Q K L A I E K S R L G I P L I F G M D V V H Query_1 382 GGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCAT 471 2 181 .......................................................................................... 270 G Y E T I F P I P L G L S C S W D M E A I R K S A R V A A I Query_1 472 GGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATC 561 2 271 .......................................................................................... 360 E A S A D G I S W T F S P M V D I S R D P R W G R V S E G N Query_1 562 GAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAAC 651 2 361 .......................................................................................... 450 G E D P F L G G A I A K A M V S G Y Q G I D L N N Q L K R N Query_1 652 GGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAAC 741 2 451 .......................................................................................... 540 D E I M A C V K H F A L Y G A G E A G R D Y N T V D M S R N Query_1 742 GATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAAC 831 2 541 .......................................................................................... 630 R M F N E Y M Y P Y Q A A V D A G V G S V M A S F N E I D G Query_1 832 CGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGC 921 2 631 .......................................................................................... 720 I P A T A N K W L M T D V L R K Q W G F D G F V V T D F T G Query_1 922 ATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGT 1011 2 721 .......................................................................................... 810 I S E M I A H G I G D L Q T V S A R A L N A G V D M D M V S Query_1 1012 ATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGT 1101 2 811 .......................................................................................... 900 E G F T G T I K K S I D E G K I S M E T L D K A C R R I L E Query_1 1102 GAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAA 1191 2 901 .......................................................................................... 990 A K Y K L G L F D N P Y K Y C D L K R P K R D I F T K E H R Query_1 1192 GCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGC 1281 2 991 .......................................................................................... 1080 D A A R K I A G E S F V L L K N D K S G S S A N P T L P L K Query_1 1282 GACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAA 1371 2 1081 .......................................................................................... 1170 K E G T V A V I G P L A N T R S N M P G T W S V A A R L N D Query_1 1372 AAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGAC 1461 2 1171 .......................................................................................... 1260 Y P S V Y E G L K E M M K G K V N I T Y A K G S N L I S D A Query_1 1462 TATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCA 1551 2 1261 .......................................................................................... 1350 A Y E E R A T M F G R S L N R D N R T D K E M L D E A L K V Query_1 1552 GCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTG 1641 2 1351 .......................................................................................... 1440 1 364 .....N...G.N......G..G...N........N....N..........N.NN..NN......NG.....N. 292 A A N A D V I I A A L G E S S E M S G E S S S R T N L A L P Query_1 1642 GCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCC 1731 2 1441 .......................................................................................... 1530 1 291 ...NN...................N.......N..N.....N.N.........N..N.....N.........................N. 202 D V Q R T L L E A L L K T G K P V V L T L F T G R P L T L T Query_1 1732 GATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACT 1821 2 1531 .......................................................................................... 1620 1 201 ...-.........N....-......N....-N...............N.-N.....-......-NN..NN...... 132 W E Q E H V P A I L N V W F G G S E A A Y A I G D V L F G D Query_1 1822 TGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGAT 1911 2 1621 .......................................................................................... 1710 V N P S G K L T M T F P K N V G Q I P L F Y N H K N T G R P Query_1 1912 GTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCT 2001 2 1711 .......................................................................................... 1800 L L E G K W F E K A R S N Y L D V D N D P L Y P F G Y G L S Query_1 2002 TTACTTGAAGGCAAATGGTTCGAAAAAGCTCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCG 2091 2 1801 ...........................TTC............................................................ 1890 Y T N F Q Y S D I T L S A P T M G Q D G S V T A M V T V T N Query_1 2092 TATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAAT 2181 2 1891 .......................................................................................... 1980 T G K Y D G A E V V Q L Y I R D L V G S I T R P V K E L K G Query_1 2182 ACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGG 2271 2 1981 .......................................................................................... 2070 F D K I F L K A G E S K T V S F K I T P E L L R F Y D Y E L Query_1 2272 TTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTC 2361 2 2071 .......................................................................................... 2160 N Y V A E P G D F D I M I G G N S Q S V K T T H L S L Query_1 2362 AACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG 2442 2 2161 ................................................................................. 2241 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 6748580 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 2,614 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115045.115 (APC115045.26-772.P(773).W437F.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVSGEIVTGQAQNSDVAKKIEQGLVGGLLNLKGVEKIRDVQKLAIEKSRLGIPLIFGMDVVHGYETIFPIPLGLSCSWDMEAIRKSARVAAIEASADGISWTFSPMVDISRDPRWGRVSEGNGEDPFLGGAIAKAMVSGYQGIDLNNQLKRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEYMYPYQAAVDAGVGSVMASFNEIDGIPATANKWLMTDVLRKQWGFDGFVVTDFTGISEMIAHGIGDLQTVSARALNAGVDMDMVSEGFTGTIKKSIDEGKISMETLDKACRRILEAKYKLGLFDNPYKYCDLKRPKRDIFTKEHRDAARKIAGESFVLLKNDKSGSSANPTLPLKKEGTVAVIGPLANTRSNMPGTWSVAARLNDYPSVYEGLKEMMKGKVNITYAKGSNLISDAAYEERATMFGRSLNRDNRTDKEMLDEALKVAANADVIIAALGESSEMSGESSSRTNLALPDVQRTLLEALLKTGKPVVLTLFTGRPLTLTWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLLEGKWFEKFRSNYLDVDNDPLYPFGYGLSYTNFQYSDITLSAPTMGQDGSVTAMVTVTNTGKYDGAEVVQLYIRDLVGSITRPVKELKGFDKIFLKAGESKTVSFKITPELLRFYDYELNYVAEPGDFDIMIGGNSQSVKTTHLSL
AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTTTCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAAGGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCATGGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATCGAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAACGGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAACGATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAACCGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGCATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGTATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGTGAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAAGCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGCGACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAAAAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGACTATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCAGCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,251 total letters Query= APC115045.115 Length=2646 Score E Sequences producing significant alignments: (Bits) Value wt 4033 0.0 K S P Q D M D R F I D A L M K K M T V E E K I G Q L N L P V Query_1 202 AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTT 291 2 1 .......................................................................................... 90 S G E I V T G Q A Q N S D V A K K I E Q G L V G G L L N L K Query_1 292 TCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAA 381 2 91 .......................................................................................... 180 G V E K I R D V Q K L A I E K S R L G I P L I F G M D V V H Query_1 382 GGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCAT 471 2 181 .......................................................................................... 270 G Y E T I F P I P L G L S C S W D M E A I R K S A R V A A I Query_1 472 GGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATC 561 2 271 .......................................................................................... 360 E A S A D G I S W T F S P M V D I S R D P R W G R V S E G N Query_1 562 GAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAAC 651 2 361 .......................................................................................... 450 G E D P F L G G A I A K A M V S G Y Q G I D L N N Q L K R N Query_1 652 GGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAAC 741 2 451 .......................................................................................... 540 D E I M A C V K H F A L Y G A G E A G R D Y N T V D M S R N Query_1 742 GATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAAC 831 2 541 .......................................................................................... 630 R M F N E Y M Y P Y Q A A V D A G V G S V M A S F N E I D G Query_1 832 CGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGC 921 2 631 .......................................................................................... 720 I P A T A N K W L M T D V L R K Q W G F D G F V V T D F T G Query_1 922 ATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGT 1011 2 721 .......................................................................................... 810 I S E M I A H G I G D L Q T V S A R A L N A G V D M D M V S Query_1 1012 ATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGT 1101 2 811 .......................................................................................... 900 E G F T G T I K K S I D E G K I S M E T L D K A C R R I L E Query_1 1102 GAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAA 1191 2 901 .......................................................................................... 990 A K Y K L G L F D N P Y K Y C D L K R P K R D I F T K E H R Query_1 1192 GCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGC 1281 2 991 .......................................................................................... 1080 D A A R K I A G E S F V L L K N D K S G S S A N P T L P L K Query_1 1282 GACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAA 1371 2 1081 .......................................................................................... 1170 K E G T V A V I G P L A N T R S N M P G T F S V A A R L N D Query_1 1372 AAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTTTAGTGTAGCCGCACGCCTCAACGAC 1461 2 1171 ................................................................GG........................ 1260 Y P S V Y E G L K E M M K G K V N I T Y A K G S N L I S D A Query_1 1462 TATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCA 1551 2 1261 .......................................................................................... 1350 A Y E E R A T M F G R S L N R D N R T D K E M L D E A L K V Query_1 1552 GCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTG 1641 2 1351 .......................................................................................... 1440 A A N A D V I I A A L G E S S E M S G E S S S R T N L A L P Query_1 1642 GCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCC 1731 2 1441 .......................................................................................... 1530 D V Q R T L L E A L L K T G K P V V L T L F T G R P L T L T Query_1 1732 GATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACT 1821 2 1531 .......................................................................................... 1620 W E Q E H V P A I L N V W F G G S E A A Y A I G D V L F G D Query_1 1822 TGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGAT 1911 2 1621 .......................................................................................... 1710 V N P S G K L T M T F P K N V G Q I P L F Y N H K N T G R P Query_1 1912 GTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCT 2001 2 1711 .......................................................................................... 1800 L L E G K W F E K F R S N Y L D V D N D P L Y P F G Y G L S Query_1 2002 TTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCG 2091 2 1801 .......................................................................................... 1890 Y T N F Q Y S D I T L S A P T M G Q D G S V T A M V T V T N Query_1 2092 TATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAAT 2181 2 1891 .......................................................................................... 1980 T G K Y D G A E V V Q L Y I R D L V G S I T R P V K E L K G Query_1 2182 ACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGG 2271 2 1981 .......................................................................................... 2070 F D K I F L K A G E S K T V S F K I T P E L L R F Y D Y E L Query_1 2272 TTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTC 2361 2 2071 .......................................................................................... 2160 N Y V A E P G D F D I M I G G N S Q S V K T T H L S L Query_1 2362 AACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG 2442 2 2161 ................................................................................. 2241 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5793890 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 2,251 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115045.116 (APC115045.26-772.P(773).E525Q.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVSGEIVTGQAQNSDVAKKIEQGLVGGLLNLKGVEKIRDVQKLAIEKSRLGIPLIFGMDVVHGYETIFPIPLGLSCSWDMEAIRKSARVAAIEASADGISWTFSPMVDISRDPRWGRVSEGNGEDPFLGGAIAKAMVSGYQGIDLNNQLKRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEYMYPYQAAVDAGVGSVMASFNEIDGIPATANKWLMTDVLRKQWGFDGFVVTDFTGISEMIAHGIGDLQTVSARALNAGVDMDMVSEGFTGTIKKSIDEGKISMETLDKACRRILEAKYKLGLFDNPYKYCDLKRPKRDIFTKEHRDAARKIAGESFVLLKNDKSGSSANPTLPLKKEGTVAVIGPLANTRSNMPGTWSVAARLNDYPSVYEGLKEMMKGKVNITYAKGSNLISDAAYEERATMFGRSLNRDNRTDKEMLDEALKVAANADVIIAALGESSEMSGESSSRTNLALPDVQRTLLEALLKTGKPVVLTLFTGRPLTLTWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLLEGKWFEKFRSNYLDVDNDPLYPFGYGLSYTNFQYSDITLSAPTMGQDGSVTAMVTVTNTGKYDGAEVVQLYIRDLVGSITRPVKELKGFDKIFLKAGESKTVSFKITPELLRFYDYELNYVAEPGDFDIMIGGNSQSVKTTHLSL
AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTTTCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAAGGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCATGGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATCGAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAACGGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAACGATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAACCGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGCATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGTATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGTGAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAAGCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGCGACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAAAAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGACTATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCAGCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,251 total letters Query= APC115045.116 Length=2646 Score E Sequences producing significant alignments: (Bits) Value wt 4033 0.0 K S P Q D M D R F I D A L M K K M T V E E K I G Q L N L P V Query_1 202 AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTT 291 2 1 .......................................................................................... 90 S G E I V T G Q A Q N S D V A K K I E Q G L V G G L L N L K Query_1 292 TCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAA 381 2 91 .......................................................................................... 180 G V E K I R D V Q K L A I E K S R L G I P L I F G M D V V H Query_1 382 GGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCAT 471 2 181 .......................................................................................... 270 G Y E T I F P I P L G L S C S W D M E A I R K S A R V A A I Query_1 472 GGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATC 561 2 271 .......................................................................................... 360 E A S A D G I S W T F S P M V D I S R D P R W G R V S E G N Query_1 562 GAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAAC 651 2 361 .......................................................................................... 450 G E D P F L G G A I A K A M V S G Y Q G I D L N N Q L K R N Query_1 652 GGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAAC 741 2 451 .......................................................................................... 540 D E I M A C V K H F A L Y G A G E A G R D Y N T V D M S R N Query_1 742 GATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAAC 831 2 541 .......................................................................................... 630 R M F N E Y M Y P Y Q A A V D A G V G S V M A S F N E I D G Query_1 832 CGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGC 921 2 631 .......................................................................................... 720 I P A T A N K W L M T D V L R K Q W G F D G F V V T D F T G Query_1 922 ATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGT 1011 2 721 .......................................................................................... 810 I S E M I A H G I G D L Q T V S A R A L N A G V D M D M V S Query_1 1012 ATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGT 1101 2 811 .......................................................................................... 900 E G F T G T I K K S I D E G K I S M E T L D K A C R R I L E Query_1 1102 GAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAA 1191 2 901 .......................................................................................... 990 A K Y K L G L F D N P Y K Y C D L K R P K R D I F T K E H R Query_1 1192 GCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGC 1281 2 991 .......................................................................................... 1080 D A A R K I A G E S F V L L K N D K S G S S A N P T L P L K Query_1 1282 GACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAA 1371 2 1081 .......................................................................................... 1170 K E G T V A V I G P L A N T R S N M P G T W S V A A R L N D Query_1 1372 AAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGAC 1461 2 1171 .......................................................................................... 1260 Y P S V Y E G L K E M M K G K V N I T Y A K G S N L I S D A Query_1 1462 TATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCA 1551 2 1261 .......................................................................................... 1350 A Y E E R A T M F G R S L N R D N R T D K E M L D E A L K V Query_1 1552 GCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTG 1641 2 1351 .......................................................................................... 1440 A A N A D V I I A A L G E S S E M S G Q S S S R T N L A L P Query_1 1642 GCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTCAGAGTTCAAGCCGCACTAACCTGGCTCTTCCC 1731 2 1441 .........................................................G.A.............................. 1530 D V Q R T L L E A L L K T G K P V V L T L F T G R P L T L T Query_1 1732 GATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACT 1821 2 1531 .......................................................................................... 1620 W E Q E H V P A I L N V W F G G S E A A Y A I G D V L F G D Query_1 1822 TGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGAT 1911 2 1621 .......................................................................................... 1710 V N P S G K L T M T F P K N V G Q I P L F Y N H K N T G R P Query_1 1912 GTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCT 2001 2 1711 .......................................................................................... 1800 L L E G K W F E K F R S N Y L D V D N D P L Y P F G Y G L S Query_1 2002 TTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCG 2091 2 1801 .......................................................................................... 1890 Y T N F Q Y S D I T L S A P T M G Q D G S V T A M V T V T N Query_1 2092 TATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAAT 2181 2 1891 .......................................................................................... 1980 T G K Y D G A E V V Q L Y I R D L V G S I T R P V K E L K G Query_1 2182 ACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGG 2271 2 1981 .......................................................................................... 2070 F D K I F L K A G E S K T V S F K I T P E L L R F Y D Y E L Query_1 2272 TTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTC 2361 2 2071 .......................................................................................... 2160 N Y V A E P G D F D I M I G G N S Q S V K T T H L S L Query_1 2362 AACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG 2442 2 2161 ................................................................................. 2241 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5793890 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 2,251 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115045.117 (APC115045.26-772.P(773).F293W.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVSGEIVTGQAQNSDVAKKIEQGLVGGLLNLKGVEKIRDVQKLAIEKSRLGIPLIFGMDVVHGYETIFPIPLGLSCSWDMEAIRKSARVAAIEASADGISWTFSPMVDISRDPRWGRVSEGNGEDPFLGGAIAKAMVSGYQGIDLNNQLKRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEYMYPYQAAVDAGVGSVMASFNEIDGIPATANKWLMTDVLRKQWGFDGFVVTDFTGISEMIAHGIGDLQTVSARALNAGVDMDMVSEGFTGTIKKSIDEGKISMETLDKACRRILEAKYKLGLFDNPYKYCDLKRPKRDIFTKEHRDAARKIAGESFVLLKNDKSGSSANPTLPLKKEGTVAVIGPLANTRSNMPGTWSVAARLNDYPSVYEGLKEMMKGKVNITYAKGSNLISDAAYEERATMFGRSLNRDNRTDKEMLDEALKVAANADVIIAALGESSEMSGESSSRTNLALPDVQRTLLEALLKTGKPVVLTLFTGRPLTLTWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLLEGKWFEKFRSNYLDVDNDPLYPFGYGLSYTNFQYSDITLSAPTMGQDGSVTAMVTVTNTGKYDGAEVVQLYIRDLVGSITRPVKELKGFDKIFLKAGESKTVSFKITPELLRFYDYELNYVAEPGDFDIMIGGNSQSVKTTHLSL
AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTTTCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAAGGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCATGGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATCGAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAACGGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAACGATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAACCGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGCATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGTATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGTGAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAAGCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGCGACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAAAAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGACTATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCAGCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,251 total letters Query= APC115045.117 Length=2646 Score E Sequences producing significant alignments: (Bits) Value wt 4033 0.0 K S P Q D M D R F I D A L M K K M T V E E K I G Q L N L P V Query_1 202 AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTT 291 2 1 .......................................................................................... 90 S G E I V T G Q A Q N S D V A K K I E Q G L V G G L L N L K Query_1 292 TCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAA 381 2 91 .......................................................................................... 180 G V E K I R D V Q K L A I E K S R L G I P L I F G M D V V H Query_1 382 GGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCAT 471 2 181 .......................................................................................... 270 G Y E T I F P I P L G L S C S W D M E A I R K S A R V A A I Query_1 472 GGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATC 561 2 271 .......................................................................................... 360 E A S A D G I S W T F S P M V D I S R D P R W G R V S E G N Query_1 562 GAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAAC 651 2 361 .......................................................................................... 450 G E D P F L G G A I A K A M V S G Y Q G I D L N N Q L K R N Query_1 652 GGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAAC 741 2 451 .......................................................................................... 540 D E I M A C V K H F A L Y G A G E A G R D Y N T V D M S R N Query_1 742 GATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAAC 831 2 541 .......................................................................................... 630 R M F N E Y M Y P Y Q A A V D A G V G S V M A S F N E I D G Query_1 832 CGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGC 921 2 631 .......................................................................................... 720 I P A T A N K W L M T D V L R K Q W G F D G F V V T D W T G Query_1 922 ATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTGGACCGGT 1011 2 721 ..................................................................................TT...... 810 I S E M I A H G I G D L Q T V S A R A L N A G V D M D M V S Query_1 1012 ATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGT 1101 2 811 .......................................................................................... 900 E G F T G T I K K S I D E G K I S M E T L D K A C R R I L E Query_1 1102 GAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAA 1191 2 901 .......................................................................................... 990 A K Y K L G L F D N P Y K Y C D L K R P K R D I F T K E H R Query_1 1192 GCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGC 1281 2 991 .......................................................................................... 1080 D A A R K I A G E S F V L L K N D K S G S S A N P T L P L K Query_1 1282 GACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAA 1371 2 1081 .......................................................................................... 1170 K E G T V A V I G P L A N T R S N M P G T W S V A A R L N D Query_1 1372 AAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGAC 1461 2 1171 .......................................................................................... 1260 Y P S V Y E G L K E M M K G K V N I T Y A K G S N L I S D A Query_1 1462 TATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCA 1551 2 1261 .......................................................................................... 1350 A Y E E R A T M F G R S L N R D N R T D K E M L D E A L K V Query_1 1552 GCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTG 1641 2 1351 .......................................................................................... 1440 A A N A D V I I A A L G E S S E M S G E S S S R T N L A L P Query_1 1642 GCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCC 1731 2 1441 .......................................................................................... 1530 D V Q R T L L E A L L K T G K P V V L T L F T G R P L T L T Query_1 1732 GATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACT 1821 2 1531 .......................................................................................... 1620 W E Q E H V P A I L N V W F G G S E A A Y A I G D V L F G D Query_1 1822 TGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGAT 1911 2 1621 .......................................................................................... 1710 V N P S G K L T M T F P K N V G Q I P L F Y N H K N T G R P Query_1 1912 GTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCT 2001 2 1711 .......................................................................................... 1800 L L E G K W F E K F R S N Y L D V D N D P L Y P F G Y G L S Query_1 2002 TTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCG 2091 2 1801 .......................................................................................... 1890 Y T N F Q Y S D I T L S A P T M G Q D G S V T A M V T V T N Query_1 2092 TATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAAT 2181 2 1891 .......................................................................................... 1980 T G K Y D G A E V V Q L Y I R D L V G S I T R P V K E L K G Query_1 2182 ACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGG 2271 2 1981 .......................................................................................... 2070 F D K I F L K A G E S K T V S F K I T P E L L R F Y D Y E L Query_1 2272 TTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTC 2361 2 2071 .......................................................................................... 2160 N Y V A E P G D F D I M I G G N S Q S V K T T H L S L Query_1 2362 AACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG 2442 2 2161 ................................................................................. 2241 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5793890 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 2,251 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115077.110 (APC115077.27-740.P(800).E426A.pMCSG68)
REQSFDEAWLFHRGDIAEGEKQSLDDSQWRQINLPHDWSIEDIPGTNSPFTADAATEVAGGFTVGGTGWYRKHFYIDAAEKGKCIAVSFDGIYMNADIWVNDRHVANHVYGYTAFELDITDYVRFGAENLIAVRVKNEGMNCRWYTGSGIYRHTFLKITNPLHFETWGTFVTTPVATADKAEVHVQSVLANTEKVTGKVILETRIVDKNNHTVARKEQLVTLDNKEKTEVGHALEVLAPQLWSIDNPYLYQVVNRLLQDDKVIDEEYISIGIRNIAFSAENGFQLNGKSMKLKGGCIHHDNGLLGAKAFDRAEERKIELLKAAGFNALRLSHNPPSIALLNACDRLGMLVIDEAFDMWRYGHYQYDYAQYFDKLWKEDLHSMVARDRNHPSVIMWSIGNEIKNKETAEIVDICRELTGFVKTLDTTRPVTAGVNSIVDATDDFLAPLDVCGYNYCLNRYESDAKRHPDRIIYASESYASQAYDYWKGVEDHSWVIGDFIWTAFDYIGEASIGWCGYPLDKRIFPWNHANCGDLNLSGERRPQSYLRETLWSDAPVSHIVVTPPVPSFPLNPDKADWSVWDFPDVVDHWNFPGYEGKKMTVSVYSNCEQVELFLNGESLGKQENTADKKNTLVWEVPYAHGILKAVSYNKGGEVGTATLESAGKVEKIRLSADRTEIVADGNDLSYITLELVDSKGIRNQLAEELVAFSIEGDAT
CGGGAACAATCTTTCGATGAGGCATGGTTATTTCATCGTGGAGATATTGCCGAAGGAGAAAAGCAATCTTTAGATGACTCACAATGGCGTCAGATAAATCTTCCTCATGATTGGAGTATTGAAGATATTCCTGGAACCAATTCTCCTTTTACAGCGGATGCTGCAACGGAAGTTGCAGGTGGTTTTACTGTAGGTGGTACGGGATGGTATAGAAAGCACTTCTACATAGATGCGGCTGAAAAAGGTAAATGTATTGCTGTCTCTTTCGATGGAATTTATATGAATGCAGATATCTGGGTGAATGATCGCCATGTAGCCAATCATGTTTATGGATATACTGCATTTGAACTGGATATAACCGATTATGTACGTTTCGGAGCTGAAAATCTGATAGCTGTCCGTGTGAAGAATGAAGGTATGAATTGCCGTTGGTATACAGGTTCGGGTATTTACAGGCATACTTTCTTGAAGATAACCAATCCGCTTCATTTTGAAACTTGGGGAACGTTTGTCACGACTCCCGTTGCAACGGCGGATAAAGCAGAGGTACATGTACAGAGTGTTCTGGCAAATACTGAAAAAGTAACCGGAAAAGTGATTCTGGAAACGCGGATTGTAGATAAGAATAACCATACTGTAGCTCGGAAAGAGCAACTGGTAACATTGGATAACAAAGAAAAAACAGAGGTTGGCCATGCGTTGGAAGTGCTTGCTCCGCAATTATGGTCTATAGACAATCCTTACTTATATCAGGTTGTAAACCGTCTTCTGCAAGATGATAAAGTTATAGATGAGGAATATATTTCAATAGGTATACGCAATATTGCATTTAGTGCGGAGAATGGTTTCCAGCTGAATGGTAAATCCATGAAACTAAAAGGCGGATGTATCCATCATGACAATGGTCTTTTGGGTGCAAAGGCTTTTGACCGGGCAGAGGAAAGGAAAATAGAACTACTGAAAGCGGCTGGTTTCAATGCGCTGCGCTTGTCTCATAATCCTCCCTCAATCGCTTTACTCAATGCCTGCGACCGCTTAGGTATGCTGGTCATAGATGAGGCTTTTGATATGTGGCGCTATGGTCATTATCAGTATGATTATGCACAATACTTTGATAAATTGTGGAAAGAAGATTTGCATAGTATGGTTGCACGGGATAGGAATCATCCTAGTGTTATCATGTGGAGTATTGGTAATGAAATCAAGAACAAAGAAACTGCTGAAATTGTGGATATATGCAGGGAGTTGACAGGTTTTGTGAAGACGCTTGATACAACGCGGCCTGTTACGGCGGGAGTTAATTCTATTGTTGATGCAACGGATGATTTTCTGGCTCCTCTGGATGTTTGTGGTTATAATTACTGTTTAAACCGTTATGAATCGGATGCCAAACGTCATCCGGACCGTATTATCTATGCTTCGGAGTCCTACGCATCCCAGGCTTATGATTATTGGAAAGGAGTAGAAGATCATTCATGGGTGATCGGTGATTTTATCTGGACTGCTTTTGACTATATTGGTGAGGCAAGTATCGGCTGGTGTGGGTATCCGCTTGATAAACGTATTTTCCCTTGGAATCATGCCAATTGTGGTGATTTGAATCTTTCGGGCGAACGTCGTCCCCAGTCCTATTTGCGTGAAACGTTATGGAGTGATGCACCGGTATCCCATATTGTTGTGACGCCTCCTGTTCCTTCTTTTCCTCTGAATCCGGATAAGGCGGATTGGAGTGTATGGGATTTTCCGGATGTTGTGGATCATTGGAATTTCCCGGGATATGAGGGGAAAAAGATGACAGTATCTGTATACTCCAATTGTGAACAGGTTGAACTGTTCTTGAATGGGGAATCTTTAGGAAAACAAGAAAATACTGCCGATAAGAAAAATACGCTTGTCTGGGAAGTACCTTATGCTCATGGAATATTGAAAGCCGTAAGTTATAATAAAGGCGGTGAAGTGGGCACTGCAACGTTGGAAAGTGCTGGTAAGGTTGAAAAGATCAGATTATCTGCGGACAGAACGGAAATCGTAGCTGATGGTAATGATCTAAGCTATATCACATTAGAATTGGTAGATAGTAAAGGCATTAGAAATCAGTTGGCTGAAGAATTGGTAGCATTTTCTATAGAAGGAGATGCTACG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,516 total letters Query= APC115077.110 Length=2547 Score E Sequences producing significant alignments: (Bits) Value wt 3855 0.0 04G_G4_APC115077.110_G04.seq 1440 0.0 R E Q S F D E A W L F H R G D I A E G E K Q S L D D S Q W R Query_1 202 CGGGAACAATCTTTCGATGAGGCATGGTTATTTCATCGTGGAGATATTGCCGAAGGAGAAAAGCAATCTTTAGATGACTCACAATGGCGT 291 2 1 .......................................................................................... 90 Q I N L P H D W S I E D I P G T N S P F T A D A A T E V A G Query_1 292 CAGATAAATCTTCCTCATGATTGGAGTATTGAAGATATTCCTGGAACCAATTCTCCTTTTACAGCGGATGCTGCAACGGAAGTTGCAGGT 381 2 91 .......................................................................................... 180 0 717 ..........NAC..... 700 G F T V G G T G W Y R K H F Y I D A A E K G K C I A V S F D Query_1 382 GGTTTTACTGTAGGTGGTACGGGATGGTATAGAAAGCACTTCTACATAGATGCGGCTGAAAAAGGTAAATGTATTGCTGTCTCTTTCGAT 471 2 181 .......................................................................................... 270 G I Y M N A D I W V N D R H V A N H V Y G Y T A F E L D I T Query_1 472 GGAATTTATATGAATGCAGATATCTGGGTGAATGATCGCCATGTAGCCAATCATGTTTATGGATATACTGCATTTGAACTGGATATAACC 561 2 271 .......................................................................................... 360 D Y V R F G A E N L I A V R V K N E G M N C R W Y T G S G I Query_1 562 GATTATGTACGTTTCGGAGCTGAAAATCTGATAGCTGTCCGTGTGAAGAATGAAGGTATGAATTGCCGTTGGTATACAGGTTCGGGTATT 651 2 361 .......................................................................................... 450 Y R H T F L K I T N P L H F E T W G T F V T T P V A T A D K Query_1 652 TACAGGCATACTTTCTTGAAGATAACCAATCCGCTTCATTTTGAAACTTGGGGAACGTTTGTCACGACTCCCGTTGCAACGGCGGATAAA 741 2 451 .......................................................................................... 540 2 532 ............ 521 A E V H V Q S V L A N T E K V T G K V I L E T R I V D K N N Query_1 742 GCAGAGGTACATGTACAGAGTGTTCTGGCAAATACTGAAAAAGTAACCGGAAAAGTGATTCTGGAAACGCGGATTGTAGATAAGAATAAC 831 2 541 .......................................................................................... 630 H T V A R K E Q L V T L D N K E K T E V G H A L E V L A P Q Query_1 832 CATACTGTAGCTCGGAAAGAGCAACTGGTAACATTGGATAACAAAGAAAAAACAGAGGTTGGCCATGCGTTGGAAGTGCTTGCTCCGCAA 921 2 631 .......................................................................................... 720 L W S I D N P Y L Y Q V V N R L L Q D D K V I D E E Y I S I Query_1 922 TTATGGTCTATAGACAATCCTTACTTATATCAGGTTGTAAACCGTCTTCTGCAAGATGATAAAGTTATAGATGAGGAATATATTTCAATA 1011 2 721 .......................................................................................... 810 G I R N I A F S A E N G F Q L N G K S M K L K G G C I H H D Query_1 1012 GGTATACGCAATATTGCATTTAGTGCGGAGAATGGTTTCCAGCTGAATGGTAAATCCATGAAACTAAAAGGCGGATGTATCCATCATGAC 1101 2 811 .......................................................................................... 900 N G L L G A K A F D R A E E R K I E L L K A A G F N A L R L Query_1 1102 AATGGTCTTTTGGGTGCAAAGGCTTTTGACCGGGCAGAGGAAAGGAAAATAGAACTACTGAAAGCGGCTGGTTTCAATGCGCTGCGCTTG 1191 2 901 .......................................................................................... 990 S H N P P S I A L L N A C D R L G M L V I D E A F D M W R Y Query_1 1192 TCTCATAATCCTCCCTCAATCGCTTTACTCAATGCCTGCGACCGCTTAGGTATGCTGGTCATAGATGAGGCTTTTGATATGTGGCGCTAT 1281 2 991 .......................................................................................... 1080 G H Y Q Y D Y A Q Y F D K L W K E D L H S M V A R D R N H P Query_1 1282 GGTCATTATCAGTATGATTATGCACAATACTTTGATAAATTGTGGAAAGAAGATTTGCATAGTATGGTTGCACGGGATAGGAATCATCCT 1371 2 1081 .......................................................................................... 1170 0 7 ................................................................ 70 S V I M W S I G N A I K N K E T A E I V D I C R E L T G F V Query_1 1372 AGTGTTATCATGTGGAGTATTGGTAATGCTATCAAGAACAAAGAAACTGCTGAAATTGTGGATATATGCAGGGAGTTGACAGGTTTTGTG 1461 2 1171 ............................AA............................................................ 1260 0 71 .......................................................................................... 160 K T L D T T R P V T A G V N S I V D A T D D F L A P L D V C Query_1 1462 AAGACGCTTGATACAACGCGGCCTGTTACGGCGGGAGTTAATTCTATTGTTGATGCAACGGATGATTTTCTGGCTCCTCTGGATGTTTGT 1551 2 1261 .......................................................................................... 1350 0 161 .......................................................................................... 250 G Y N Y C L N R Y E S D A K R H P D R I I Y A S E S Y A S Q Query_1 1552 GGTTATAATTACTGTTTAAACCGTTATGAATCGGATGCCAAACGTCATCCGGACCGTATTATCTATGCTTCGGAGTCCTACGCATCCCAG 1641 2 1351 .......................................................................................... 1440 0 251 .......................................................................................... 340 A Y D Y W K G V E D H S W V I G D F I W T A F D Y I G E A S Query_1 1642 GCTTATGATTATTGGAAAGGAGTAGAAGATCATTCATGGGTGATCGGTGATTTTATCTGGACTGCTTTTGACTATATTGGTGAGGCAAGT 1731 2 1441 .......................................................................................... 1530 0 341 ......A................................................................................... 430 I G W C G Y P L D K R I F P W N H A N C G D L N L S G E R R Query_1 1732 ATCGGCTGGTGTGGGTATCCGCTTGATAAACGTATTTTCCCTTGGAATCATGCCAATTGTGGTGATTTGAATCTTTCGGGCGAACGTCGT 1821 2 1531 .......................................................................................... 1620 0 431 .......................................................................................... 520 P Q S Y L R E T L W S D A P V S H I V V T P P V P S F P L N Query_1 1822 CCCCAGTCCTATTTGCGTGAAACGTTATGGAGTGATGCACCGGTATCCCATATTGTTGTGACGCCTCCTGTTCCTTCTTTTCCTCTGAAT 1911 2 1621 .......................................................................................... 1710 0 521 .......................................................................................... 610 P D K A D W S V W D F P D V V D H W N F P G Y E G K K M T Query_1 1912 CCGGATAAGGCGGATTGGAGTGTATGGGATTTTCCGGATGTTGTGGATCATTGGAATTTCCCGGGATATGAGGGGAAAAAGATGACAG-T 2000 2 1711 ........................................................................................-. 1799 0 611 ...........................N..........N.........................N.......................N. 700 V S V Y S N C E Q V E L F L N G E S L G K Q E N T A D K K Query_1 2001 ATCTGT-ATACTCCAATTGTGAACAGGTTGAA-CTGTTCTTGAATGG-GGAATCTTTAGGAAAACAAGAAAATACTGCCGATAAGAAAAA 2087 2 1800 ......-.........................-..............-.......................................... 1886 0 701 ......N................NC.......C...N..........N...............................N.......... 790 N T L V W E V P Y A H G I L K A V S Y N K G G E V G T A Query_1 2088 TACGCTTGTCTGGG-AAGTACCTT-ATGCTCATGGAATATTGAA-AGCCGTAAGTTATAATAAAGG--CGGTGAAGTGGGC-ACTGCAAC 2171 2 1887 ..............-.........-...................-.....................--.............-........ 1970 0 791 .....C..C.N...A.........A.NC................TN...N......N...N...N.NCN..GN.TN.....NN.....C. 880 T L E S A G K V E K I R L S A D R T E I V A D G N D L S Y Query_1 2172 GTTGGAAA-GTGC-TGGTAA-GGTTGAAAAGATCAGATTATCTGCGGACAG-AACGGAAATCGTAGCTGATGGTAATGATCTAAGCTATA 2257 2 1971 ........-....-......-..............................-...................................... 2056 0 881 TN......T....NN.....N.N..N........GA....N.N......N.N.N........ 942 I T L E L V D S K G I R N Q L A E E L V A F S I E G D A T * Query_1 2258 TCACATTAGAATTGGTAGATAGTAAAGGCATTAGAAATCAGTTGGCTGAAGAATTGGTAGCATTTTCTATAGAAGGAGATGCTACGTAAC 2347 2 2057 ...................................................................................... 2142 2 658 ..... 662 H W Query_1 2348 ATTGGA 2353 2 663 ...... 668 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 8766450 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 3,516 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115077.111 (APC115077.27-740.P(800).Y480A.pMCSG68)
REQSFDEAWLFHRGDIAEGEKQSLDDSQWRQINLPHDWSIEDIPGTNSPFTADAATEVAGGFTVGGTGWYRKHFYIDAAEKGKCIAVSFDGIYMNADIWVNDRHVANHVYGYTAFELDITDYVRFGAENLIAVRVKNEGMNCRWYTGSGIYRHTFLKITNPLHFETWGTFVTTPVATADKAEVHVQSVLANTEKVTGKVILETRIVDKNNHTVARKEQLVTLDNKEKTEVGHALEVLAPQLWSIDNPYLYQVVNRLLQDDKVIDEEYISIGIRNIAFSAENGFQLNGKSMKLKGGCIHHDNGLLGAKAFDRAEERKIELLKAAGFNALRLSHNPPSIALLNACDRLGMLVIDEAFDMWRYGHYQYDYAQYFDKLWKEDLHSMVARDRNHPSVIMWSIGNEIKNKETAEIVDICRELTGFVKTLDTTRPVTAGVNSIVDATDDFLAPLDVCGYNYCLNRYESDAKRHPDRIIYASESYASQAYDYWKGVEDHSWVIGDFIWTAFDYIGEASIGWCGYPLDKRIFPWNHANCGDLNLSGERRPQSYLRETLWSDAPVSHIVVTPPVPSFPLNPDKADWSVWDFPDVVDHWNFPGYEGKKMTVSVYSNCEQVELFLNGESLGKQENTADKKNTLVWEVPYAHGILKAVSYNKGGEVGTATLESAGKVEKIRLSADRTEIVADGNDLSYITLELVDSKGIRNQLAEELVAFSIEGDAT
CGGGAACAATCTTTCGATGAGGCATGGTTATTTCATCGTGGAGATATTGCCGAAGGAGAAAAGCAATCTTTAGATGACTCACAATGGCGTCAGATAAATCTTCCTCATGATTGGAGTATTGAAGATATTCCTGGAACCAATTCTCCTTTTACAGCGGATGCTGCAACGGAAGTTGCAGGTGGTTTTACTGTAGGTGGTACGGGATGGTATAGAAAGCACTTCTACATAGATGCGGCTGAAAAAGGTAAATGTATTGCTGTCTCTTTCGATGGAATTTATATGAATGCAGATATCTGGGTGAATGATCGCCATGTAGCCAATCATGTTTATGGATATACTGCATTTGAACTGGATATAACCGATTATGTACGTTTCGGAGCTGAAAATCTGATAGCTGTCCGTGTGAAGAATGAAGGTATGAATTGCCGTTGGTATACAGGTTCGGGTATTTACAGGCATACTTTCTTGAAGATAACCAATCCGCTTCATTTTGAAACTTGGGGAACGTTTGTCACGACTCCCGTTGCAACGGCGGATAAAGCAGAGGTACATGTACAGAGTGTTCTGGCAAATACTGAAAAAGTAACCGGAAAAGTGATTCTGGAAACGCGGATTGTAGATAAGAATAACCATACTGTAGCTCGGAAAGAGCAACTGGTAACATTGGATAACAAAGAAAAAACAGAGGTTGGCCATGCGTTGGAAGTGCTTGCTCCGCAATTATGGTCTATAGACAATCCTTACTTATATCAGGTTGTAAACCGTCTTCTGCAAGATGATAAAGTTATAGATGAGGAATATATTTCAATAGGTATACGCAATATTGCATTTAGTGCGGAGAATGGTTTCCAGCTGAATGGTAAATCCATGAAACTAAAAGGCGGATGTATCCATCATGACAATGGTCTTTTGGGTGCAAAGGCTTTTGACCGGGCAGAGGAAAGGAAAATAGAACTACTGAAAGCGGCTGGTTTCAATGCGCTGCGCTTGTCTCATAATCCTCCCTCAATCGCTTTACTCAATGCCTGCGACCGCTTAGGTATGCTGGTCATAGATGAGGCTTTTGATATGTGGCGCTATGGTCATTATCAGTATGATTATGCACAATACTTTGATAAATTGTGGAAAGAAGATTTGCATAGTATGGTTGCACGGGATAGGAATCATCCTAGTGTTATCATGTGGAGTATTGGTAATGAAATCAAGAACAAAGAAACTGCTGAAATTGTGGATATATGCAGGGAGTTGACAGGTTTTGTGAAGACGCTTGATACAACGCGGCCTGTTACGGCGGGAGTTAATTCTATTGTTGATGCAACGGATGATTTTCTGGCTCCTCTGGATGTTTGTGGTTATAATTACTGTTTAAACCGTTATGAATCGGATGCCAAACGTCATCCGGACCGTATTATCTATGCTTCGGAGTCCTACGCATCCCAGGCTTATGATTATTGGAAAGGAGTAGAAGATCATTCATGGGTGATCGGTGATTTTATCTGGACTGCTTTTGACTATATTGGTGAGGCAAGTATCGGCTGGTGTGGGTATCCGCTTGATAAACGTATTTTCCCTTGGAATCATGCCAATTGTGGTGATTTGAATCTTTCGGGCGAACGTCGTCCCCAGTCCTATTTGCGTGAAACGTTATGGAGTGATGCACCGGTATCCCATATTGTTGTGACGCCTCCTGTTCCTTCTTTTCCTCTGAATCCGGATAAGGCGGATTGGAGTGTATGGGATTTTCCGGATGTTGTGGATCATTGGAATTTCCCGGGATATGAGGGGAAAAAGATGACAGTATCTGTATACTCCAATTGTGAACAGGTTGAACTGTTCTTGAATGGGGAATCTTTAGGAAAACAAGAAAATACTGCCGATAAGAAAAATACGCTTGTCTGGGAAGTACCTTATGCTCATGGAATATTGAAAGCCGTAAGTTATAATAAAGGCGGTGAAGTGGGCACTGCAACGTTGGAAAGTGCTGGTAAGGTTGAAAAGATCAGATTATCTGCGGACAGAACGGAAATCGTAGCTGATGGTAATGATCTAAGCTATATCACATTAGAATTGGTAGATAGTAAAGGCATTAGAAATCAGTTGGCTGAAGAATTGGTAGCATTTTCTATAGAAGGAGATGCTACG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,152 total letters Query= APC115077.111 Length=2547 Score E Sequences producing significant alignments: (Bits) Value wt 3849 0.0 R E Q S F D E A W L F H R G D I A E G E K Q S L D D S Q W R Query_1 202 CGGGAACAATCTTTCGATGAGGCATGGTTATTTCATCGTGGAGATATTGCCGAAGGAGAAAAGCAATCTTTAGATGACTCACAATGGCGT 291 2 1 .......................................................................................... 90 Q I N L P H D W S I E D I P G T N S P F T A D A A T E V A G Query_1 292 CAGATAAATCTTCCTCATGATTGGAGTATTGAAGATATTCCTGGAACCAATTCTCCTTTTACAGCGGATGCTGCAACGGAAGTTGCAGGT 381 2 91 .......................................................................................... 180 G F T V G G T G W Y R K H F Y I D A A E K G K C I A V S F D Query_1 382 GGTTTTACTGTAGGTGGTACGGGATGGTATAGAAAGCACTTCTACATAGATGCGGCTGAAAAAGGTAAATGTATTGCTGTCTCTTTCGAT 471 2 181 .......................................................................................... 270 G I Y M N A D I W V N D R H V A N H V Y G Y T A F E L D I T Query_1 472 GGAATTTATATGAATGCAGATATCTGGGTGAATGATCGCCATGTAGCCAATCATGTTTATGGATATACTGCATTTGAACTGGATATAACC 561 2 271 .......................................................................................... 360 D Y V R F G A E N L I A V R V K N E G M N C R W Y T G S G I Query_1 562 GATTATGTACGTTTCGGAGCTGAAAATCTGATAGCTGTCCGTGTGAAGAATGAAGGTATGAATTGCCGTTGGTATACAGGTTCGGGTATT 651 2 361 .......................................................................................... 450 Y R H T F L K I T N P L H F E T W G T F V T T P V A T A D K Query_1 652 TACAGGCATACTTTCTTGAAGATAACCAATCCGCTTCATTTTGAAACTTGGGGAACGTTTGTCACGACTCCCGTTGCAACGGCGGATAAA 741 2 451 .......................................................................................... 540 2 532 ............ 521 A E V H V Q S V L A N T E K V T G K V I L E T R I V D K N N Query_1 742 GCAGAGGTACATGTACAGAGTGTTCTGGCAAATACTGAAAAAGTAACCGGAAAAGTGATTCTGGAAACGCGGATTGTAGATAAGAATAAC 831 2 541 .......................................................................................... 630 H T V A R K E Q L V T L D N K E K T E V G H A L E V L A P Q Query_1 832 CATACTGTAGCTCGGAAAGAGCAACTGGTAACATTGGATAACAAAGAAAAAACAGAGGTTGGCCATGCGTTGGAAGTGCTTGCTCCGCAA 921 2 631 .......................................................................................... 720 L W S I D N P Y L Y Q V V N R L L Q D D K V I D E E Y I S I Query_1 922 TTATGGTCTATAGACAATCCTTACTTATATCAGGTTGTAAACCGTCTTCTGCAAGATGATAAAGTTATAGATGAGGAATATATTTCAATA 1011 2 721 .......................................................................................... 810 G I R N I A F S A E N G F Q L N G K S M K L K G G C I H H D Query_1 1012 GGTATACGCAATATTGCATTTAGTGCGGAGAATGGTTTCCAGCTGAATGGTAAATCCATGAAACTAAAAGGCGGATGTATCCATCATGAC 1101 2 811 .......................................................................................... 900 N G L L G A K A F D R A E E R K I E L L K A A G F N A L R L Query_1 1102 AATGGTCTTTTGGGTGCAAAGGCTTTTGACCGGGCAGAGGAAAGGAAAATAGAACTACTGAAAGCGGCTGGTTTCAATGCGCTGCGCTTG 1191 2 901 .......................................................................................... 990 S H N P P S I A L L N A C D R L G M L V I D E A F D M W R Y Query_1 1192 TCTCATAATCCTCCCTCAATCGCTTTACTCAATGCCTGCGACCGCTTAGGTATGCTGGTCATAGATGAGGCTTTTGATATGTGGCGCTAT 1281 2 991 .......................................................................................... 1080 G H Y Q Y D Y A Q Y F D K L W K E D L H S M V A R D R N H P Query_1 1282 GGTCATTATCAGTATGATTATGCACAATACTTTGATAAATTGTGGAAAGAAGATTTGCATAGTATGGTTGCACGGGATAGGAATCATCCT 1371 2 1081 .......................................................................................... 1170 S V I M W S I G N E I K N K E T A E I V D I C R E L T G F V Query_1 1372 AGTGTTATCATGTGGAGTATTGGTAATGAAATCAAGAACAAAGAAACTGCTGAAATTGTGGATATATGCAGGGAGTTGACAGGTTTTGTG 1461 2 1171 .......................................................................................... 1260 K T L D T T R P V T A G V N S I V D A T D D F L A P L D V C Query_1 1462 AAGACGCTTGATACAACGCGGCCTGTTACGGCGGGAGTTAATTCTATTGTTGATGCAACGGATGATTTTCTGGCTCCTCTGGATGTTTGT 1551 2 1261 .......................................................................................... 1350 G Y N A C L N R Y E S D A K R H P D R I I Y A S E S Y A S Q Query_1 1552 GGTTATAATGCTTGTTTAAACCGTTATGAATCGGATGCCAAACGTCATCCGGACCGTATTATCTATGCTTCGGAGTCCTACGCATCCCAG 1641 2 1351 .........TAC.............................................................................. 1440 A Y D Y W K G V E D H S W V I G D F I W T A F D Y I G E A S Query_1 1642 GCTTATGATTATTGGAAAGGAGTAGAAGATCATTCATGGGTGATCGGTGATTTTATCTGGACTGCTTTTGACTATATTGGTGAGGCAAGT 1731 2 1441 .......................................................................................... 1530 I G W C G Y P L D K R I F P W N H A N C G D L N L S G E R R Query_1 1732 ATCGGCTGGTGTGGGTATCCGCTTGATAAACGTATTTTCCCTTGGAATCATGCCAATTGTGGTGATTTGAATCTTTCGGGCGAACGTCGT 1821 2 1531 .......................................................................................... 1620 P Q S Y L R E T L W S D A P V S H I V V T P P V P S F P L N Query_1 1822 CCCCAGTCCTATTTGCGTGAAACGTTATGGAGTGATGCACCGGTATCCCATATTGTTGTGACGCCTCCTGTTCCTTCTTTTCCTCTGAAT 1911 2 1621 .......................................................................................... 1710 P D K A D W S V W D F P D V V D H W N F P G Y E G K K M T V Query_1 1912 CCGGATAAGGCGGATTGGAGTGTATGGGATTTTCCGGATGTTGTGGATCATTGGAATTTCCCGGGATATGAGGGGAAAAAGATGACAGTA 2001 2 1711 .......................................................................................... 1800 S V Y S N C E Q V E L F L N G E S L G K Q E N T A D K K N T Query_1 2002 TCTGTATACTCCAATTGTGAACAGGTTGAACTGTTCTTGAATGGGGAATCTTTAGGAAAACAAGAAAATACTGCCGATAAGAAAAATACG 2091 2 1801 .......................................................................................... 1890 L V W E V P Y A H G I L K A V S Y N K G G E V G T A T L E S Query_1 2092 CTTGTCTGGGAAGTACCTTATGCTCATGGAATATTGAAAGCCGTAAGTTATAATAAAGGCGGTGAAGTGGGCACTGCAACGTTGGAAAGT 2181 2 1891 .......................................................................................... 1980 A G K V E K I R L S A D R T E I V A D G N D L S Y I T L E L Query_1 2182 GCTGGTAAGGTTGAAAAGATCAGATTATCTGCGGACAGAACGGAAATCGTAGCTGATGGTAATGATCTAAGCTATATCACATTAGAATTG 2271 2 1981 .......................................................................................... 2070 V D S K G I R N Q L A E E L V A F S I E G D A T * H W Query_1 2272 GTAGATAGTAAAGGCATTAGAAATCAGTTGGCTGAAGAATTGGTAGCATTTTCTATAGAAGGAGATGCTACGTAACATTGGA 2353 2 2071 ........................................................................ 2142 2 658 ........... 668 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5325224 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 2,152 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115077.114 (APC115077.27-740.P(800).E426Q.pMCSG68)
REQSFDEAWLFHRGDIAEGEKQSLDDSQWRQINLPHDWSIEDIPGTNSPFTADAATEVAGGFTVGGTGWYRKHFYIDAAEKGKCIAVSFDGIYMNADIWVNDRHVANHVYGYTAFELDITDYVRFGAENLIAVRVKNEGMNCRWYTGSGIYRHTFLKITNPLHFETWGTFVTTPVATADKAEVHVQSVLANTEKVTGKVILETRIVDKNNHTVARKEQLVTLDNKEKTEVGHALEVLAPQLWSIDNPYLYQVVNRLLQDDKVIDEEYISIGIRNIAFSAENGFQLNGKSMKLKGGCIHHDNGLLGAKAFDRAEERKIELLKAAGFNALRLSHNPPSIALLNACDRLGMLVIDEAFDMWRYGHYQYDYAQYFDKLWKEDLHSMVARDRNHPSVIMWSIGNEIKNKETAEIVDICRELTGFVKTLDTTRPVTAGVNSIVDATDDFLAPLDVCGYNYCLNRYESDAKRHPDRIIYASESYASQAYDYWKGVEDHSWVIGDFIWTAFDYIGEASIGWCGYPLDKRIFPWNHANCGDLNLSGERRPQSYLRETLWSDAPVSHIVVTPPVPSFPLNPDKADWSVWDFPDVVDHWNFPGYEGKKMTVSVYSNCEQVELFLNGESLGKQENTADKKNTLVWEVPYAHGILKAVSYNKGGEVGTATLESAGKVEKIRLSADRTEIVADGNDLSYITLELVDSKGIRNQLAEELVAFSIEGDAT
CGGGAACAATCTTTCGATGAGGCATGGTTATTTCATCGTGGAGATATTGCCGAAGGAGAAAAGCAATCTTTAGATGACTCACAATGGCGTCAGATAAATCTTCCTCATGATTGGAGTATTGAAGATATTCCTGGAACCAATTCTCCTTTTACAGCGGATGCTGCAACGGAAGTTGCAGGTGGTTTTACTGTAGGTGGTACGGGATGGTATAGAAAGCACTTCTACATAGATGCGGCTGAAAAAGGTAAATGTATTGCTGTCTCTTTCGATGGAATTTATATGAATGCAGATATCTGGGTGAATGATCGCCATGTAGCCAATCATGTTTATGGATATACTGCATTTGAACTGGATATAACCGATTATGTACGTTTCGGAGCTGAAAATCTGATAGCTGTCCGTGTGAAGAATGAAGGTATGAATTGCCGTTGGTATACAGGTTCGGGTATTTACAGGCATACTTTCTTGAAGATAACCAATCCGCTTCATTTTGAAACTTGGGGAACGTTTGTCACGACTCCCGTTGCAACGGCGGATAAAGCAGAGGTACATGTACAGAGTGTTCTGGCAAATACTGAAAAAGTAACCGGAAAAGTGATTCTGGAAACGCGGATTGTAGATAAGAATAACCATACTGTAGCTCGGAAAGAGCAACTGGTAACATTGGATAACAAAGAAAAAACAGAGGTTGGCCATGCGTTGGAAGTGCTTGCTCCGCAATTATGGTCTATAGACAATCCTTACTTATATCAGGTTGTAAACCGTCTTCTGCAAGATGATAAAGTTATAGATGAGGAATATATTTCAATAGGTATACGCAATATTGCATTTAGTGCGGAGAATGGTTTCCAGCTGAATGGTAAATCCATGAAACTAAAAGGCGGATGTATCCATCATGACAATGGTCTTTTGGGTGCAAAGGCTTTTGACCGGGCAGAGGAAAGGAAAATAGAACTACTGAAAGCGGCTGGTTTCAATGCGCTGCGCTTGTCTCATAATCCTCCCTCAATCGCTTTACTCAATGCCTGCGACCGCTTAGGTATGCTGGTCATAGATGAGGCTTTTGATATGTGGCGCTATGGTCATTATCAGTATGATTATGCACAATACTTTGATAAATTGTGGAAAGAAGATTTGCATAGTATGGTTGCACGGGATAGGAATCATCCTAGTGTTATCATGTGGAGTATTGGTAATGAAATCAAGAACAAAGAAACTGCTGAAATTGTGGATATATGCAGGGAGTTGACAGGTTTTGTGAAGACGCTTGATACAACGCGGCCTGTTACGGCGGGAGTTAATTCTATTGTTGATGCAACGGATGATTTTCTGGCTCCTCTGGATGTTTGTGGTTATAATTACTGTTTAAACCGTTATGAATCGGATGCCAAACGTCATCCGGACCGTATTATCTATGCTTCGGAGTCCTACGCATCCCAGGCTTATGATTATTGGAAAGGAGTAGAAGATCATTCATGGGTGATCGGTGATTTTATCTGGACTGCTTTTGACTATATTGGTGAGGCAAGTATCGGCTGGTGTGGGTATCCGCTTGATAAACGTATTTTCCCTTGGAATCATGCCAATTGTGGTGATTTGAATCTTTCGGGCGAACGTCGTCCCCAGTCCTATTTGCGTGAAACGTTATGGAGTGATGCACCGGTATCCCATATTGTTGTGACGCCTCCTGTTCCTTCTTTTCCTCTGAATCCGGATAAGGCGGATTGGAGTGTATGGGATTTTCCGGATGTTGTGGATCATTGGAATTTCCCGGGATATGAGGGGAAAAAGATGACAGTATCTGTATACTCCAATTGTGAACAGGTTGAACTGTTCTTGAATGGGGAATCTTTAGGAAAACAAGAAAATACTGCCGATAAGAAAAATACGCTTGTCTGGGAAGTACCTTATGCTCATGGAATATTGAAAGCCGTAAGTTATAATAAAGGCGGTGAAGTGGGCACTGCAACGTTGGAAAGTGCTGGTAAGGTTGAAAAGATCAGATTATCTGCGGACAGAACGGAAATCGTAGCTGATGGTAATGATCTAAGCTATATCACATTAGAATTGGTAGATAGTAAAGGCATTAGAAATCAGTTGGCTGAAGAATTGGTAGCATTTTCTATAGAAGGAGATGCTACG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,472 total letters Query= APC115077.114 Length=2547 Score E Sequences producing significant alignments: (Bits) Value wt 3855 0.0 11A_A5_APC115077.114_A05.seq 1243 0.0 R E Q S F D E A W L F H R G D I A E G E K Q S L D D S Q W R Query_1 202 CGGGAACAATCTTTCGATGAGGCATGGTTATTTCATCGTGGAGATATTGCCGAAGGAGAAAAGCAATCTTTAGATGACTCACAATGGCGT 291 2 1 .......................................................................................... 90 Q I N L P H D W S I E D I P G T N S P F T A D A A T E V A G Query_1 292 CAGATAAATCTTCCTCATGATTGGAGTATTGAAGATATTCCTGGAACCAATTCTCCTTTTACAGCGGATGCTGCAACGGAAGTTGCAGGT 381 2 91 .......................................................................................... 180 G F T V G G T G W Y R K H F Y I D A A E K G K C I A V S F D Query_1 382 GGTTTTACTGTAGGTGGTACGGGATGGTATAGAAAGCACTTCTACATAGATGCGGCTGAAAAAGGTAAATGTATTGCTGTCTCTTTCGAT 471 2 181 .......................................................................................... 270 G I Y M N A D I W V N D R H V A N H V Y G Y T A F E L D I T Query_1 472 GGAATTTATATGAATGCAGATATCTGGGTGAATGATCGCCATGTAGCCAATCATGTTTATGGATATACTGCATTTGAACTGGATATAACC 561 2 271 .......................................................................................... 360 D Y V R F G A E N L I A V R V K N E G M N C R W Y T G S G I Query_1 562 GATTATGTACGTTTCGGAGCTGAAAATCTGATAGCTGTCCGTGTGAAGAATGAAGGTATGAATTGCCGTTGGTATACAGGTTCGGGTATT 651 2 361 .......................................................................................... 450 Y R H T F L K I T N P L H F E T W G T F V T T P V A T A D K Query_1 652 TACAGGCATACTTTCTTGAAGATAACCAATCCGCTTCATTTTGAAACTTGGGGAACGTTTGTCACGACTCCCGTTGCAACGGCGGATAAA 741 2 451 .......................................................................................... 540 2 532 ............ 521 A E V H V Q S V L A N T E K V T G K V I L E T R I V D Query_1 742 GCAGAGGTACATGTACAGAGTGTTCTGGCAAATACTGAAAAAGT-AACC--GGAAAAGTGATTCTGGAAACGC--GGATTGTA--GATAA 824 2 541 ............................................-....--......................--........--..... 623 1 967 ......NG....CG......N..N...G......C.CG..T.NN.NCG.T... 915 K N N H T V A R K E Q L V T L D N K E K T E V G H A Query_1 825 GAATAA--CCATA--CTGTAGCTCGGAAA--GAGC-AACT-GGTAACATTGG-ATAA-CAAAGAAAAAA--CAGAGGTT-GGCC-ATGCG 900 2 624 ......--.....--..............--....-....-...........-....-...........--........-....-..... 699 1 914 ....T.AC....GAC.NT..T..G.....ANN...C....T......N....G....A...N....N..AC.NN.....N....TN.... 825 L E V L A P Q L W S I D N P Y L Y Q V V N R L L Q D D Query_1 901 TTGGAAGT-GCTTGC--TCCGCAATTATGGTCTAT-AGACAATC-CTTACTTATATCAGGTTGTAAA-CCG-TCTTCTG-CAAG-ATGAT 981 2 700 ........-......--..................-........-......................-...-.......-....-..... 780 1 824 .......NN..N..GCT..........N.....N.T........N..N......N..G..GN.GN..G...N.......N....N..... 735 K V I D E E Y I S I G I R N I A F S A E N G F Q L N G K Query_1 982 AAAGT-TATAGATG-AGGAATATATTTCAATAGGTATACGCAATATTGCATTTAGTGCGGAGAATGGTTTCCAGCTGAAT--GGTAAATC 1067 2 781 .....-........-.................................................................--........ 866 1 734 .N..NN........G.....NG........................N....N.N.....................GN..ATG.....N.. 645 S M K L K G G C I H H D N G L L G A K A F D R A E E R K I E Query_1 1068 CATGAAACTAAAAGGCGGATGTATCCATCATG-ACAATGGTC-TTTTGGGTGCAAAGGCTTTTGACCGGGCAGAGGAAAGGAAAATAGAA 1155 2 867 ................................-.........-............................................... 954 1 644 ................................T.........C..N...........................N...............N 555 L L K A A G F N A L R L S H N P P S I A L L N A C D R L G Query_1 1156 CTACTGAAAGCGGCTGGTTTCAATGCGCTGCGCTTGTCTCATAATCCTCCCTCAATCG-CTTTACTCAATGCCTGCGACCGCTTAGGTAT 1244 2 955 ..........................................................-............................... 1043 1 554 ..........................................................G.N............................. 465 M L V I D E A F D M W R Y G H Y Q Y D Y A Q Y F D K L W K E Query_1 1245 GCTGGTCATAGATGAGGCTTTTGATATGTGGCGCTATGGTCATTATCAGTATGATTATGCACAATACTTTGATAAATTGTGGAAAGAAGA 1334 2 1044 .......................................................................................... 1133 1 464 .......................................................................................... 375 D L H S M V A R D R N H P S V I M W S I G N Q I K N K E T A Query_1 1335 TTTGCATAGTATGGTTGCACGGGATAGGAATCATCCTAGTGTTATCATGTGGAGTATTGGTAATCAGATCAAGAACAAAGAAACTGCTGA 1424 2 1134 ................................................................G.A....................... 1223 1 374 .......................................................................................... 285 E I V D I C R E L T G F V K T L D T T R P V T A G V N S I V Query_1 1425 AATTGTGGATATATGCAGGGAGTTGACAGGTTTTGTGAAGACGCTTGATACAACGCGGCCTGTTACGGCGGGAGTTAATTCTATTGTTGA 1514 2 1224 .......................................................................................... 1313 1 284 .......................................................................................... 195 D A T D D F L A P L D V C G Y N Y C L N R Y E S D A K R H P Query_1 1515 TGCAACGGATGATTTTCTGGCTCCTCTGGATGTTTGTGGTTATAATTACTGTTTAAACCGTTATGAATCGGATGCCAAACGTCATCCGGA 1604 2 1314 .......................................................................................... 1403 1 194 .......................................................................................... 105 D R I I Y A S E S Y A S Q A Y D Y W K G V E D H S W V I G D Query_1 1605 CCGTATTATCTATGCTTCGGAGTCCTACGCATCCCAGGCTTATGATTATTGGAAAGGAGTAGAAGATCATTCATGGGTGAT-CGGTGATT 1693 2 1404 .................................................................................-........ 1492 1 104 ...........................................A.............................-N...N..N......-. 17 F I W T A F D Y I G E A S I G W C G Y P L D K R I F P W N H Query_1 1694 TTATCTGGACTGCTTTTGACTATATTGGTGAGGCAAGTATCGGCTGGTGTGGGTATCCGCTTGATAAACGTATTTTCCCTTGGAATCATG 1783 2 1493 .......................................................................................... 1582 1 16 ........ 9 A N C G D L N L S G E R R P Q S Y L R E T L W S D A P V S H Query_1 1784 CCAATTGTGGTGATTTGAATCTTTCGGGCGAACGTCGTCCCCAGTCCTATTTGCGTGAAACGTTATGGAGTGATGCACCGGTATCCCATA 1873 2 1583 .......................................................................................... 1672 I V V T P P V P S F P L N P D K A D W S V W D F P D V V D H Query_1 1874 TTGTTGTGACGCCTCCTGTTCCTTCTTTTCCTCTGAATCCGGATAAGGCGGATTGGAGTGTATGGGATTTTCCGGATGTTGTGGATCATT 1963 2 1673 .......................................................................................... 1762 W N F P G Y E G K K M T V S V Y S N C E Q V E L F L N G E S Query_1 1964 GGAATTTCCCGGGATATGAGGGGAAAAAGATGACAGTATCTGTATACTCCAATTGTGAACAGGTTGAACTGTTCTTGAATGGGGAATCTT 2053 2 1763 .......................................................................................... 1852 L G K Q E N T A D K K N T L V W E V P Y A H G I L K A V S Y Query_1 2054 TAGGAAAACAAGAAAATACTGCCGATAAGAAAAATACGCTTGTCTGGGAAGTACCTTATGCTCATGGAATATTGAAAGCCGTAAGTTATA 2143 2 1853 .......................................................................................... 1942 N K G G E V G T A T L E S A G K V E K I R L S A D R T E I V Query_1 2144 ATAAAGGCGGTGAAGTGGGCACTGCAACGTTGGAAAGTGCTGGTAAGGTTGAAAAGATCAGATTATCTGCGGACAGAACGGAAATCGTAG 2233 2 1943 .......................................................................................... 2032 A D G N D L S Y I T L E L V D S K G I R N Q L A E E L V A F Query_1 2234 CTGATGGTAATGATCTAAGCTATATCACATTAGAATTGGTAGATAGTAAAGGCATTAGAAATCAGTTGGCTGAAGAATTGGTAGCATTTT 2323 2 2033 .......................................................................................... 2122 S I E G D A T * H W Query_1 2324 CTATAGAAGGAGATGCTACGTAACATTGGA 2353 2 2123 .................... 2142 2 658 ........... 668 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 8655130 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 3,472 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115077.115 (APC115077.27-740.P(800).E501Q.pMCSG68)
REQSFDEAWLFHRGDIAEGEKQSLDDSQWRQINLPHDWSIEDIPGTNSPFTADAATEVAGGFTVGGTGWYRKHFYIDAAEKGKCIAVSFDGIYMNADIWVNDRHVANHVYGYTAFELDITDYVRFGAENLIAVRVKNEGMNCRWYTGSGIYRHTFLKITNPLHFETWGTFVTTPVATADKAEVHVQSVLANTEKVTGKVILETRIVDKNNHTVARKEQLVTLDNKEKTEVGHALEVLAPQLWSIDNPYLYQVVNRLLQDDKVIDEEYISIGIRNIAFSAENGFQLNGKSMKLKGGCIHHDNGLLGAKAFDRAEERKIELLKAAGFNALRLSHNPPSIALLNACDRLGMLVIDEAFDMWRYGHYQYDYAQYFDKLWKEDLHSMVARDRNHPSVIMWSIGNEIKNKETAEIVDICRELTGFVKTLDTTRPVTAGVNSIVDATDDFLAPLDVCGYNYCLNRYESDAKRHPDRIIYASESYASQAYDYWKGVEDHSWVIGDFIWTAFDYIGEASIGWCGYPLDKRIFPWNHANCGDLNLSGERRPQSYLRETLWSDAPVSHIVVTPPVPSFPLNPDKADWSVWDFPDVVDHWNFPGYEGKKMTVSVYSNCEQVELFLNGESLGKQENTADKKNTLVWEVPYAHGILKAVSYNKGGEVGTATLESAGKVEKIRLSADRTEIVADGNDLSYITLELVDSKGIRNQLAEELVAFSIEGDAT
CGGGAACAATCTTTCGATGAGGCATGGTTATTTCATCGTGGAGATATTGCCGAAGGAGAAAAGCAATCTTTAGATGACTCACAATGGCGTCAGATAAATCTTCCTCATGATTGGAGTATTGAAGATATTCCTGGAACCAATTCTCCTTTTACAGCGGATGCTGCAACGGAAGTTGCAGGTGGTTTTACTGTAGGTGGTACGGGATGGTATAGAAAGCACTTCTACATAGATGCGGCTGAAAAAGGTAAATGTATTGCTGTCTCTTTCGATGGAATTTATATGAATGCAGATATCTGGGTGAATGATCGCCATGTAGCCAATCATGTTTATGGATATACTGCATTTGAACTGGATATAACCGATTATGTACGTTTCGGAGCTGAAAATCTGATAGCTGTCCGTGTGAAGAATGAAGGTATGAATTGCCGTTGGTATACAGGTTCGGGTATTTACAGGCATACTTTCTTGAAGATAACCAATCCGCTTCATTTTGAAACTTGGGGAACGTTTGTCACGACTCCCGTTGCAACGGCGGATAAAGCAGAGGTACATGTACAGAGTGTTCTGGCAAATACTGAAAAAGTAACCGGAAAAGTGATTCTGGAAACGCGGATTGTAGATAAGAATAACCATACTGTAGCTCGGAAAGAGCAACTGGTAACATTGGATAACAAAGAAAAAACAGAGGTTGGCCATGCGTTGGAAGTGCTTGCTCCGCAATTATGGTCTATAGACAATCCTTACTTATATCAGGTTGTAAACCGTCTTCTGCAAGATGATAAAGTTATAGATGAGGAATATATTTCAATAGGTATACGCAATATTGCATTTAGTGCGGAGAATGGTTTCCAGCTGAATGGTAAATCCATGAAACTAAAAGGCGGATGTATCCATCATGACAATGGTCTTTTGGGTGCAAAGGCTTTTGACCGGGCAGAGGAAAGGAAAATAGAACTACTGAAAGCGGCTGGTTTCAATGCGCTGCGCTTGTCTCATAATCCTCCCTCAATCGCTTTACTCAATGCCTGCGACCGCTTAGGTATGCTGGTCATAGATGAGGCTTTTGATATGTGGCGCTATGGTCATTATCAGTATGATTATGCACAATACTTTGATAAATTGTGGAAAGAAGATTTGCATAGTATGGTTGCACGGGATAGGAATCATCCTAGTGTTATCATGTGGAGTATTGGTAATGAAATCAAGAACAAAGAAACTGCTGAAATTGTGGATATATGCAGGGAGTTGACAGGTTTTGTGAAGACGCTTGATACAACGCGGCCTGTTACGGCGGGAGTTAATTCTATTGTTGATGCAACGGATGATTTTCTGGCTCCTCTGGATGTTTGTGGTTATAATTACTGTTTAAACCGTTATGAATCGGATGCCAAACGTCATCCGGACCGTATTATCTATGCTTCGGAGTCCTACGCATCCCAGGCTTATGATTATTGGAAAGGAGTAGAAGATCATTCATGGGTGATCGGTGATTTTATCTGGACTGCTTTTGACTATATTGGTGAGGCAAGTATCGGCTGGTGTGGGTATCCGCTTGATAAACGTATTTTCCCTTGGAATCATGCCAATTGTGGTGATTTGAATCTTTCGGGCGAACGTCGTCCCCAGTCCTATTTGCGTGAAACGTTATGGAGTGATGCACCGGTATCCCATATTGTTGTGACGCCTCCTGTTCCTTCTTTTCCTCTGAATCCGGATAAGGCGGATTGGAGTGTATGGGATTTTCCGGATGTTGTGGATCATTGGAATTTCCCGGGATATGAGGGGAAAAAGATGACAGTATCTGTATACTCCAATTGTGAACAGGTTGAACTGTTCTTGAATGGGGAATCTTTAGGAAAACAAGAAAATACTGCCGATAAGAAAAATACGCTTGTCTGGGAAGTACCTTATGCTCATGGAATATTGAAAGCCGTAAGTTATAATAAAGGCGGTGAAGTGGGCACTGCAACGTTGGAAAGTGCTGGTAAGGTTGAAAAGATCAGATTATCTGCGGACAGAACGGAAATCGTAGCTGATGGTAATGATCTAAGCTATATCACATTAGAATTGGTAGATAGTAAAGGCATTAGAAATCAGTTGGCTGAAGAATTGGTAGCATTTTCTATAGAAGGAGATGCTACG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,761 total letters Query= APC115077.115 Length=2547 Score E Sequences producing significant alignments: (Bits) Value wt 3858 0.0 05B_B5_APC115077.115_B05.seq 1721 0.0 11B_B5_APC115077.115_B05.seq 1131 0.0 R E Q S F D E A W L F H R G D I A E G E K Q S L D D S Q W R Query_1 202 CGGGAACAATCTTTCGATGAGGCATGGTTATTTCATCGTGGAGATATTGCCGAAGGAGAAAAGCAATCTTTAGATGACTCACAATGGCGT 291 2 1 .......................................................................................... 90 1 7 .. 8 Q I N L P H D W S I E D I P G T N S P F T A D A A T E V A G Query_1 292 CAGATAAATCTTCCTCATGATTGGAGTATTGAAGATATTCCTGGAACCAATTCTCCTTTTACAGCGGATGCTGCAACGGAAGTTGCAGGT 381 2 91 .......................................................................................... 180 1 9 ......... 17 G F T V G G T G W Y R K H F Y I D A A E K G K C I A V S F D Query_1 382 GGTTTTACTGTAGGTGGTACGGGATGGTATAGAAAGCACTTCTACATAGATGCGGCTGAAAAAGGTAAATGTATTGCTGTCTCTTTCGAT 471 2 181 .......................................................................................... 270 G I Y M N A D I W V N D R H V A N H V Y G Y T A F E L D I T Query_1 472 GGAATTTATATGAATGCAGATATCTGGGTGAATGATCGCCATGTAGCCAATCATGTTTATGGATATACTGCATTTGAACTGGATATAACC 561 2 271 .......................................................................................... 360 D Y V R F G A E N L I A V R V K N E G M N C R W Y T G S G I Query_1 562 GATTATGTACGTTTCGGAGCTGAAAATCTGATAGCTGTCCGTGTGAAGAATGAAGGTATGAATTGCCGTTGGTATACAGGTTCGGGTATT 651 2 361 .......................................................................................... 450 Y R H T F L K I T N P L H F E T W G T F V T T P V A T A D K Query_1 652 TACAGGCATACTTTCTTGAAGATAACCAATCCGCTTCATTTTGAAACTTGGGGAACGTTTGTCACGACTCCCGTTGCAACGGCGGATAAA 741 2 451 .......................................................................................... 540 2 532 ............ 521 A E V H V Q S V L A N T E K V T G K V I L E T R I V D K N N Query_1 742 GCAGAGGTACATGTACAGAGTGTTCTGGCAAATACTGAAAAAGTAACCGGAAAAGTGATTCTGGAAACGCGGATTGTAGATAAGAATAAC 831 2 541 .......................................................................................... 630 H T V A R K E Q L V T L D N K E K T E V G H A L E V L A P Query_1 832 CATACTGTAGCTCGGAAAGAGCAACTGGTAACATTGGATAACAAAGAAAAAACAGAGGTTGGCCATGCGTTGGAAGTGC-TTGCT-CCGC 919 2 631 ...............................................................................-.....-.... 718 1 817 .......C.....N.... 800 Q L W S I D N P Y L Y Q V V N R L L Q D D K V I D E Query_1 920 AA-TTATGGT-CTAT-AGACAATCCTTAC-TTATAT--CAGGTTGTAAA--CCGTC-TTCTGCAAG-ATGATAAA-GTTATAGATGAGGA 998 2 719 ..-.......-....-.............-......--...........--.....-.........-........-.............. 797 1 799 ..G...N...N...NN.A........NG.N..N.NANC...N...NN.NTC.....G..N.N...NN.G.N....N..NN...N..G... 710 E Y I S I G I R N I A F S A E N G F Q L N G K S M K L K G G Query_1 999 ATATATTTCAATAGGTATACGCAATATTGCATTT-AGTGCGGAGAATGGTTTCCAGCTG-AATGGTAAATCCATGAAACTAAAAGGCGGA 1086 2 798 ..................................-........................-.............................. 885 1 709 ....NN................NN.G.N....NNN...N.............N....N.G...............GT..........N.. 620 C I H H D N G L L G A K A F D R A E E R K I E L L K A A G F Query_1 1087 TGTATCCATCATGACAATGGTCTTTTGGGTGCAAAGGCTTTTGACCGGGCAGAGGAAAGGAAAATAGAACTACTGAAAGCGGCTGGTTTC 1176 2 886 .......................................................................................... 975 1 619 .......G.....N...........N..............N............N.................................... 530 N A L R L S H N P P S I A L L N A C D R L G M L V I D E A F Query_1 1177 AATGCGCTGCGCTTGTCTCATAATCCTCCCTCAATCGCTTTACTCAATGCCTGCGACCGCTTAGGTATGCTGGTCATAGATGAGGCTTTT 1266 2 976 .......................................................................................... 1065 1 529 ..........................................................N.............................N. 440 D M W R Y G H Y Q Y D Y A Q Y F D K L W K E D L H S M V A R Query_1 1267 GATATGTGGCGCTATGGTCATTATCAGTATGATTATGCACAATACTTTGATAAATTGTGGAAAGAAGATTTGCATAGTATGGTTGCACGG 1356 2 1066 .......................................................................................... 1155 0 26 ..N.......-.................................... 71 1 439 ..............................................G........................................... 350 D R N H P S V I M W S I G N E I K N K E T A E I V D I C R E Query_1 1357 GATAGGAATCATCCTAGTGTTATCATGTGGAGTATTGGTAATGAAATCAAGAACAAAGAAACTGCTGAAATTGTGGATATATGCAGGGAG 1446 2 1156 .......................................................................................... 1245 0 72 .......................................................................................... 161 1 349 .........................................N................................................ 260 L T G F V K T L D T T R P V T A G V N S I V D A T D D F L A Query_1 1447 TTGACAGGTTTTGTGAAGACGCTTGATACAACGCGGCCTGTTACGGCGGGAGTTAATTCTATTGTTGATGCAACGGATGATTTTCTGGCT 1536 2 1246 .......................................................................................... 1335 0 162 .......................................................................................... 251 1 259 .......................................................................................... 170 P L D V C G Y N Y C L N R Y E S D A K R H P D R I I Y A S Q Query_1 1537 CCTCTGGATGTTTGTGGTTATAATTACTGTTTAAACCGTTATGAATCGGATGCCAAACGTCATCCGGACCGTATTATCTATGCTTCGCAG 1626 2 1336 .......................................................................................G.. 1425 0 252 .......................................................................................... 341 1 169 ............N...................................................................N......... 80 S Y A S Q A Y D Y W K G V E D H S W V I G D F I W T A F D Y Query_1 1627 TCCTACGCATCCCAGGCTTATGATTATTGGAAAGGAGTAGAAGATCATTCATGGGTGATCGGTGATTTTATCTGGACTGCTTTTGACTAT 1716 2 1426 .......................................................................................... 1515 0 342 .......................................................................................... 431 1 79 ............N...........N..N.......................N...-N....--.NA........ 9 I G E A S I G W C G Y P L D K R I F P W N H A N C G D L N L Query_1 1717 ATTGGTGAGGCAAGTATCGGCTGGTGTGGGTATCCGCTTGATAAACGTATTTTCCCTTGGAATCATGCCAATTGTGGTGATTTGAATCTT 1806 2 1516 .......................................................................................... 1605 0 432 .......................................................................................... 521 S G E R R P Q S Y L R E T L W S D A P V S H I V V T P P V P Query_1 1807 TCGGGCGAACGTCGTCCCCAGTCCTATTTGCGTGAAACGTTATGGAGTGATGCACCGGTATCCCATATTGTTGTGACGCCTCCTGTTCCT 1896 2 1606 .......................................................................................... 1695 0 522 .......................................................................................... 611 S F P L N P D K A D W S V W D F P D V V D H W N F P G Y E G Query_1 1897 TCTTTTCCTCTGAATCCGGATAAGGCGGATTGGAGTGTATGGGATTTTCCGGATGTTGTGGATCATTGGAATTTCCCGGGATATGAGGGG 1986 2 1696 .......................................................................................... 1785 0 612 .......................................................................................... 701 K K M T V S V Y S N C E Q V E L F L N G E S L G K Q E N T A Query_1 1987 AAAAAGATGACAGTATCTGTATACTCCAATTGTGAACAGGTTGAACTGTTCTTGAATGGGGAATCTTTAGGAAAACAAGAAAATACTGCC 2076 2 1786 .......................................................................................... 1875 0 702 .......................................................................................... 791 D K K N T L V W E V P Y A H G I L K A V S Y N K G G E V G Query_1 2077 GATAAGAAAAATACGCTTGTCTGGG-AAGTACCTTATGCTCATGGAATATTGAAAGCCGTAAGTTATAATAAAGGCGGTGAAGTGGGCAC 2165 2 1876 .........................-................................................................ 1964 0 792 .........................N..................................-......N..N................... 880 T A T L E S A G K V E K I R L S A D R T E I V A D G N D L S Query_1 2166 TGCAACGTTGGAAAGTGCTGGTAAGGTTGAAAAGATCAGATTATCTGCGGACAGAACGGAAATCGTAGCTGAT-GGTAATGATCTAAGCT 2254 2 1965 .........................................................................-................ 2053 0 881 ...N...N.......N.....GT..............N...................N.........N.....G..N.N..N...N.... 970 Y I T L E L V D S K G I R N Q L A E E L V A F S I E G D A Query_1 2255 ATATCACATTAGAATTGGTAGATAGTAAAGGCATT-AGAAATCAGTTGGCTGAAG-AATTGGTAGCATTTTCTATAGAAGGAG-ATGCTA 2341 2 2054 ...................................-...................-...........................-...... 2140 0 971 C.........TA..A.T......N...N.......N.............NNN...A........N.T.N.NNN..N......NA...... 1060 T * H W K W I T D P N S S S V D K L A A A Query_1 2342 CG-TAACATTGGAAGTGGATAACGG-ATCCGAATTCGAG-CTCCGTCGACAAGCTTGCGGC-CGCA 2403 2 2141 .. 2142 2 658 .-.......... 668 0 1061 ..N..N....-.C.N.N........N.AT..........N...GC....N.....N..T..N.... 1125 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11916300 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 4,761 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115077.118 (APC115077.27-740.P(800).E501A.pMCSG68)
REQSFDEAWLFHRGDIAEGEKQSLDDSQWRQINLPHDWSIEDIPGTNSPFTADAATEVAGGFTVGGTGWYRKHFYIDAAEKGKCIAVSFDGIYMNADIWVNDRHVANHVYGYTAFELDITDYVRFGAENLIAVRVKNEGMNCRWYTGSGIYRHTFLKITNPLHFETWGTFVTTPVATADKAEVHVQSVLANTEKVTGKVILETRIVDKNNHTVARKEQLVTLDNKEKTEVGHALEVLAPQLWSIDNPYLYQVVNRLLQDDKVIDEEYISIGIRNIAFSAENGFQLNGKSMKLKGGCIHHDNGLLGAKAFDRAEERKIELLKAAGFNALRLSHNPPSIALLNACDRLGMLVIDEAFDMWRYGHYQYDYAQYFDKLWKEDLHSMVARDRNHPSVIMWSIGNEIKNKETAEIVDICRELTGFVKTLDTTRPVTAGVNSIVDATDDFLAPLDVCGYNYCLNRYESDAKRHPDRIIYASESYASQAYDYWKGVEDHSWVIGDFIWTAFDYIGEASIGWCGYPLDKRIFPWNHANCGDLNLSGERRPQSYLRETLWSDAPVSHIVVTPPVPSFPLNPDKADWSVWDFPDVVDHWNFPGYEGKKMTVSVYSNCEQVELFLNGESLGKQENTADKKNTLVWEVPYAHGILKAVSYNKGGEVGTATLESAGKVEKIRLSADRTEIVADGNDLSYITLELVDSKGIRNQLAEELVAFSIEGDAT
CGGGAACAATCTTTCGATGAGGCATGGTTATTTCATCGTGGAGATATTGCCGAAGGAGAAAAGCAATCTTTAGATGACTCACAATGGCGTCAGATAAATCTTCCTCATGATTGGAGTATTGAAGATATTCCTGGAACCAATTCTCCTTTTACAGCGGATGCTGCAACGGAAGTTGCAGGTGGTTTTACTGTAGGTGGTACGGGATGGTATAGAAAGCACTTCTACATAGATGCGGCTGAAAAAGGTAAATGTATTGCTGTCTCTTTCGATGGAATTTATATGAATGCAGATATCTGGGTGAATGATCGCCATGTAGCCAATCATGTTTATGGATATACTGCATTTGAACTGGATATAACCGATTATGTACGTTTCGGAGCTGAAAATCTGATAGCTGTCCGTGTGAAGAATGAAGGTATGAATTGCCGTTGGTATACAGGTTCGGGTATTTACAGGCATACTTTCTTGAAGATAACCAATCCGCTTCATTTTGAAACTTGGGGAACGTTTGTCACGACTCCCGTTGCAACGGCGGATAAAGCAGAGGTACATGTACAGAGTGTTCTGGCAAATACTGAAAAAGTAACCGGAAAAGTGATTCTGGAAACGCGGATTGTAGATAAGAATAACCATACTGTAGCTCGGAAAGAGCAACTGGTAACATTGGATAACAAAGAAAAAACAGAGGTTGGCCATGCGTTGGAAGTGCTTGCTCCGCAATTATGGTCTATAGACAATCCTTACTTATATCAGGTTGTAAACCGTCTTCTGCAAGATGATAAAGTTATAGATGAGGAATATATTTCAATAGGTATACGCAATATTGCATTTAGTGCGGAGAATGGTTTCCAGCTGAATGGTAAATCCATGAAACTAAAAGGCGGATGTATCCATCATGACAATGGTCTTTTGGGTGCAAAGGCTTTTGACCGGGCAGAGGAAAGGAAAATAGAACTACTGAAAGCGGCTGGTTTCAATGCGCTGCGCTTGTCTCATAATCCTCCCTCAATCGCTTTACTCAATGCCTGCGACCGCTTAGGTATGCTGGTCATAGATGAGGCTTTTGATATGTGGCGCTATGGTCATTATCAGTATGATTATGCACAATACTTTGATAAATTGTGGAAAGAAGATTTGCATAGTATGGTTGCACGGGATAGGAATCATCCTAGTGTTATCATGTGGAGTATTGGTAATGAAATCAAGAACAAAGAAACTGCTGAAATTGTGGATATATGCAGGGAGTTGACAGGTTTTGTGAAGACGCTTGATACAACGCGGCCTGTTACGGCGGGAGTTAATTCTATTGTTGATGCAACGGATGATTTTCTGGCTCCTCTGGATGTTTGTGGTTATAATTACTGTTTAAACCGTTATGAATCGGATGCCAAACGTCATCCGGACCGTATTATCTATGCTTCGGAGTCCTACGCATCCCAGGCTTATGATTATTGGAAAGGAGTAGAAGATCATTCATGGGTGATCGGTGATTTTATCTGGACTGCTTTTGACTATATTGGTGAGGCAAGTATCGGCTGGTGTGGGTATCCGCTTGATAAACGTATTTTCCCTTGGAATCATGCCAATTGTGGTGATTTGAATCTTTCGGGCGAACGTCGTCCCCAGTCCTATTTGCGTGAAACGTTATGGAGTGATGCACCGGTATCCCATATTGTTGTGACGCCTCCTGTTCCTTCTTTTCCTCTGAATCCGGATAAGGCGGATTGGAGTGTATGGGATTTTCCGGATGTTGTGGATCATTGGAATTTCCCGGGATATGAGGGGAAAAAGATGACAGTATCTGTATACTCCAATTGTGAACAGGTTGAACTGTTCTTGAATGGGGAATCTTTAGGAAAACAAGAAAATACTGCCGATAAGAAAAATACGCTTGTCTGGGAAGTACCTTATGCTCATGGAATATTGAAAGCCGTAAGTTATAATAAAGGCGGTGAAGTGGGCACTGCAACGTTGGAAAGTGCTGGTAAGGTTGAAAAGATCAGATTATCTGCGGACAGAACGGAAATCGTAGCTGATGGTAATGATCTAAGCTATATCACATTAGAATTGGTAGATAGTAAAGGCATTAGAAATCAGTTGGCTGAAGAATTGGTAGCATTTTCTATAGAAGGAGATGCTACG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,898 total letters Query= APC115077.118 Length=2547 Score E Sequences producing significant alignments: (Bits) Value wt 3855 0.0 05C_C5_APC115077.118_C05.seq 1393 0.0 11C_C5_APC115077.118_C05.seq 1355 0.0 R E Q S F D E A W L F H R G D I A E G E K Q S L D D S Q W R Query_1 202 CGGGAACAATCTTTCGATGAGGCATGGTTATTTCATCGTGGAGATATTGCCGAAGGAGAAAAGCAATCTTTAGATGACTCACAATGGCGT 291 2 1 .......................................................................................... 90 Q I N L P H D W S I E D I P G T N S P F T A D A A T E V A G Query_1 292 CAGATAAATCTTCCTCATGATTGGAGTATTGAAGATATTCCTGGAACCAATTCTCCTTTTACAGCGGATGCTGCAACGGAAGTTGCAGGT 381 2 91 .......................................................................................... 180 G F T V G G T G W Y R K H F Y I D A A E K G K C I A V S F D Query_1 382 GGTTTTACTGTAGGTGGTACGGGATGGTATAGAAAGCACTTCTACATAGATGCGGCTGAAAAAGGTAAATGTATTGCTGTCTCTTTCGAT 471 2 181 .......................................................................................... 270 G I Y M N A D I W V N D R H V A N H V Y G Y T A F E L D I T Query_1 472 GGAATTTATATGAATGCAGATATCTGGGTGAATGATCGCCATGTAGCCAATCATGTTTATGGATATACTGCATTTGAACTGGATATAACC 561 2 271 .......................................................................................... 360 D Y V R F G A E N L I A V R V K N E G M N C R W Y T G S G I Query_1 562 GATTATGTACGTTTCGGAGCTGAAAATCTGATAGCTGTCCGTGTGAAGAATGAAGGTATGAATTGCCGTTGGTATACAGGTTCGGGTATT 651 2 361 .......................................................................................... 450 Y R H T F L K I T N P L H F E T W G T F V T T P V A T A D K Query_1 652 TACAGGCATACTTTCTTGAAGATAACCAATCCGCTTCATTTTGAAACTTGGGGAACGTTTGTCACGACTCCCGTTGCAACGGCGGATAAA 741 2 451 .......................................................................................... 540 2 532 ............ 521 A E V H V Q S V L A N T E K V T G K V I L E T R I V D K N Query_1 742 GCAGAGGTACATGTACAGAGTGTTCTGGCAAATACTGAAAAAGTAACCGGAAAAGTGATTCTGGAAACGCGGA-TTGTA-GATAA-GAAT 828 2 541 .........................................................................-.....-.....-.... 627 1 933 ....N....G.....T.....A...N 908 N H T V A R K E Q L V T L D N K E K T E V G H A L Query_1 829 AA--CCATAC-TGTAGC--TCGGAAAG--AGC-AACTGGTAA-CATTGGATAACAAAG----AAAAAACAG-AGGTTGGCC-ATGCGTTG 903 2 628 ..--......-......--........--...-.........-...............----.........-.........-........ 702 1 907 ..AC....T.CN....GCT.....N.NGA...N.........N.N.N.......N..NGNAA.G...C...G...G.....C.N...... 818 E V L A P Q L W S I D N P Y L Y Q V V N R L L Q D D Query_1 904 G-AAG-TGC-TTGCTCC-GCAATTAT-GGTCTATAGACAATCCTT--ACTT-ATATCA-GGTTGTAAA-CCGTCTTCT-GCAAGA-TGAT 981 2 703 .-...-...-.......-........-..................--....-......-.........-.........-......-.... 780 1 817 .G...N...C.......C........T....N...........N.TA....T.....NN.........A.......T.T......A.... 728 K V I D E E Y I S I G I R N I A F S A E N G F Q L N G K S Query_1 982 AAAGTTATAGATGA-GGAATATATTTCAATAGGTATACGCAATATT-GCATTTAGTGCGGAGAATGGTTTCCAGCTGAA-TGGTAAATCC 1068 2 781 ..............-...............................-................................-.......... 867 1 727 ..............A...............................N................................A.......... 638 M K L K G G C I H H D N G L L G A K A F D R A E E R K I E L Query_1 1069 ATGAAACTAAAAGGCGGATGTATCCATCATGACAATGGTCTTTTGGGTGCAAAGGCTTTTGACCGGGCAGAGGAAAGGAAAATAGAACTA 1158 2 868 .......................................................................................... 957 1 637 .......................................................................................... 548 L K A A G F N A L R L S H N P P S I A L L N A C D R L G M L Query_1 1159 CTGAAAGCGGCTGGTTTCAATGCGCTGCGCTTGTCTCATAATCCTCCCTCAATCGCTTTACTCAATGCCTGCGACCGCTTAGGTATGCTG 1248 2 958 .......................................................................................... 1047 1 547 .......................................................................................... 458 V I D E A F D M W R Y G H Y Q Y D Y A Q Y F D K L W K E D L Query_1 1249 GTCATAGATGAGGCTTTTGATATGTGGCGCTATGGTCATTATCAGTATGATTATGCACAATACTTTGATAAATTGTGGAAAGAAGATTTG 1338 2 1048 .......................................................................................... 1137 0 2 ......................... 26 1 457 .......................................................................................... 368 H S M V A R D R N H P S V I M W S I G N E I K N K E T A E I Query_1 1339 CATAGTATGGTTGCACGGGATAGGAATCATCCTAGTGTTATCATGTGGAGTATTGGTAATGAAATCAAGAACAAAGAAACTGCTGAAATT 1428 2 1138 .......................................................................................... 1227 0 27 .......................................................................................... 116 1 367 .......................................................................................... 278 V D I C R E L T G F V K T L D T T R P V T A G V N S I V D A Query_1 1429 GTGGATATATGCAGGGAGTTGACAGGTTTTGTGAAGACGCTTGATACAACGCGGCCTGTTACGGCGGGAGTTAATTCTATTGTTGATGCA 1518 2 1228 .......................................................................................... 1317 0 117 .......................................................................................... 206 1 277 .......................................................................................... 188 T D D F L A P L D V C G Y N Y C L N R Y E S D A K R H P D R Query_1 1519 ACGGATGATTTTCTGGCTCCTCTGGATGTTTGTGGTTATAATTACTGTTTAAACCGTTATGAATCGGATGCCAAACGTCATCCGGACCGT 1608 2 1318 .......................................................................................... 1407 0 207 .......................................................................................... 296 1 187 .......................................................................................... 98 I I Y A S A S Y A S Q A Y D Y W K G V E D H S W V I G D F Query_1 1609 ATTATCTATGCTTCGGCTTCCTACGCATCCCAGGCTTATGATTATTGGAAAGGAGTAGAAGATCATTCATGGGTGATC-GGTGATTTTAT 1697 2 1408 ................AG............................................................-........... 1496 0 297 ..............................................................................-........... 385 1 97 ..............................................................................C........-.. 9 I W T A F D Y I G E A S I G W C G Y P L D K R I F P W N H A Query_1 1698 CTGGACTGCTTTTGACTATATTGGTGAGGCAAGTATCGGCTGGTGTGGGTATCCGCTTGATAAACGTATTTTCCCTTGGAATCATGCCAA 1787 2 1497 .......................................................................................... 1586 0 386 .......................................................................................... 475 1 8 ...... 3 N C G D L N L S G E R R P Q S Y L R E T L W S D A P V S H I Query_1 1788 TTGTGGTGATTTGAATCTTTCGGGCGAACGTCGTCCCCAGTCCTATTTGCGTGAAACGTTATGGAGTGATGCACCGGTATCCCATATTGT 1877 2 1587 .......................................................................................... 1676 0 476 ..........................................................N............................... 565 V V T P P V P S F P L N P D K A D W S V W D F P D V V D H W Query_1 1878 TGTGACGCCTCCTGTTCCTTCTTTTCCTCTGAATCCGGATAAGGCGGATTGGAGTGTATGGGATTTTCCGGATGTTGTGGATCATTGGAA 1967 2 1677 .......................................................................................... 1766 0 566 ...............N...............................................................C.......... 655 N F P G Y E G K K M T V S V Y S N C E Q V E L F L N G E S L Query_1 1968 TTTCCCGGGATATGAGGGGAAAAAGATGACAGTATCTGTATACTCCAATTGTGAACAGGTTGAACTGTT-CTTGAAT-GGGGAATCTTTA 2055 2 1767 .....................................................................-.......-............ 1854 0 656 ...............N........................N.......N........N.....C.....C.......G............ 745 G K Q E N T A D K K N T L V W E V P Y A H G I L K A V Query_1 2056 GGA--AAACAAG-AAAATACTGCCGATAAG-AAAAATACGCTTGTCTGGGAAGTA-CCTTATGCTCAT-GGAATA--TTGAAAGCCGTAA 2137 2 1855 ...--.......-.................-........................-............-......--............. 1936 0 746 N..AN.......A....N...........NA........C....N..........C.....N......G......AT.G.......N... 835 S Y N K G G E V G T A T L E S A G K V E K I R L S A D Query_1 2138 GTTA-TAATAAAGGC--GGTGAAGTGG-GCACTG-CAACGTTGG--AAAGTGC-TGGTAAGGTTGAAAAGATCAGATTATCTGCGGACAG 2219 2 1937 ....-..........--..........-......-.........--.......-.................................... 2018 0 836 N...T..........CG..G...NC..CC.....T....C.N..GA...NN..NN.N.....N.N...... 906 R T E I V A D G N D L S Y I T L E L V D S K G I R N Q L A E Query_1 2220 AACGGAAATCGTAGCTGATGGTAATGATCTAAGCTATATCACATTAGAATTGGTAGATAGTAAAGGCATTAGAAATCAGTTGGCTGAAGA 2309 2 2019 .......................................................................................... 2108 E L V A F S I E G D A T * H W K W I T D P N S S S V D K L A Query_1 2310 ATTGGTAGCATTTTCTATAGAAGGAGATGCTACGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGC 2399 2 2109 .................................. 2142 2 658 ........... 668 A A L E H H H H H H * D P A A N K A R K E A E L A A A T A E Query_1 2400 CGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCA 2489 Q * L A * P L G A Query_1 2490 ATAACTAGCATAACCCCTTGGGGCCTC 2516 0 1223 ............. 1235 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12262910 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 4,898 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115077.119 (APC115077.27-740.P(800).Y480F.pMCSG68)
REQSFDEAWLFHRGDIAEGEKQSLDDSQWRQINLPHDWSIEDIPGTNSPFTADAATEVAGGFTVGGTGWYRKHFYIDAAEKGKCIAVSFDGIYMNADIWVNDRHVANHVYGYTAFELDITDYVRFGAENLIAVRVKNEGMNCRWYTGSGIYRHTFLKITNPLHFETWGTFVTTPVATADKAEVHVQSVLANTEKVTGKVILETRIVDKNNHTVARKEQLVTLDNKEKTEVGHALEVLAPQLWSIDNPYLYQVVNRLLQDDKVIDEEYISIGIRNIAFSAENGFQLNGKSMKLKGGCIHHDNGLLGAKAFDRAEERKIELLKAAGFNALRLSHNPPSIALLNACDRLGMLVIDEAFDMWRYGHYQYDYAQYFDKLWKEDLHSMVARDRNHPSVIMWSIGNEIKNKETAEIVDICRELTGFVKTLDTTRPVTAGVNSIVDATDDFLAPLDVCGYNYCLNRYESDAKRHPDRIIYASESYASQAYDYWKGVEDHSWVIGDFIWTAFDYIGEASIGWCGYPLDKRIFPWNHANCGDLNLSGERRPQSYLRETLWSDAPVSHIVVTPPVPSFPLNPDKADWSVWDFPDVVDHWNFPGYEGKKMTVSVYSNCEQVELFLNGESLGKQENTADKKNTLVWEVPYAHGILKAVSYNKGGEVGTATLESAGKVEKIRLSADRTEIVADGNDLSYITLELVDSKGIRNQLAEELVAFSIEGDAT
CGGGAACAATCTTTCGATGAGGCATGGTTATTTCATCGTGGAGATATTGCCGAAGGAGAAAAGCAATCTTTAGATGACTCACAATGGCGTCAGATAAATCTTCCTCATGATTGGAGTATTGAAGATATTCCTGGAACCAATTCTCCTTTTACAGCGGATGCTGCAACGGAAGTTGCAGGTGGTTTTACTGTAGGTGGTACGGGATGGTATAGAAAGCACTTCTACATAGATGCGGCTGAAAAAGGTAAATGTATTGCTGTCTCTTTCGATGGAATTTATATGAATGCAGATATCTGGGTGAATGATCGCCATGTAGCCAATCATGTTTATGGATATACTGCATTTGAACTGGATATAACCGATTATGTACGTTTCGGAGCTGAAAATCTGATAGCTGTCCGTGTGAAGAATGAAGGTATGAATTGCCGTTGGTATACAGGTTCGGGTATTTACAGGCATACTTTCTTGAAGATAACCAATCCGCTTCATTTTGAAACTTGGGGAACGTTTGTCACGACTCCCGTTGCAACGGCGGATAAAGCAGAGGTACATGTACAGAGTGTTCTGGCAAATACTGAAAAAGTAACCGGAAAAGTGATTCTGGAAACGCGGATTGTAGATAAGAATAACCATACTGTAGCTCGGAAAGAGCAACTGGTAACATTGGATAACAAAGAAAAAACAGAGGTTGGCCATGCGTTGGAAGTGCTTGCTCCGCAATTATGGTCTATAGACAATCCTTACTTATATCAGGTTGTAAACCGTCTTCTGCAAGATGATAAAGTTATAGATGAGGAATATATTTCAATAGGTATACGCAATATTGCATTTAGTGCGGAGAATGGTTTCCAGCTGAATGGTAAATCCATGAAACTAAAAGGCGGATGTATCCATCATGACAATGGTCTTTTGGGTGCAAAGGCTTTTGACCGGGCAGAGGAAAGGAAAATAGAACTACTGAAAGCGGCTGGTTTCAATGCGCTGCGCTTGTCTCATAATCCTCCCTCAATCGCTTTACTCAATGCCTGCGACCGCTTAGGTATGCTGGTCATAGATGAGGCTTTTGATATGTGGCGCTATGGTCATTATCAGTATGATTATGCACAATACTTTGATAAATTGTGGAAAGAAGATTTGCATAGTATGGTTGCACGGGATAGGAATCATCCTAGTGTTATCATGTGGAGTATTGGTAATGAAATCAAGAACAAAGAAACTGCTGAAATTGTGGATATATGCAGGGAGTTGACAGGTTTTGTGAAGACGCTTGATACAACGCGGCCTGTTACGGCGGGAGTTAATTCTATTGTTGATGCAACGGATGATTTTCTGGCTCCTCTGGATGTTTGTGGTTATAATTACTGTTTAAACCGTTATGAATCGGATGCCAAACGTCATCCGGACCGTATTATCTATGCTTCGGAGTCCTACGCATCCCAGGCTTATGATTATTGGAAAGGAGTAGAAGATCATTCATGGGTGATCGGTGATTTTATCTGGACTGCTTTTGACTATATTGGTGAGGCAAGTATCGGCTGGTGTGGGTATCCGCTTGATAAACGTATTTTCCCTTGGAATCATGCCAATTGTGGTGATTTGAATCTTTCGGGCGAACGTCGTCCCCAGTCCTATTTGCGTGAAACGTTATGGAGTGATGCACCGGTATCCCATATTGTTGTGACGCCTCCTGTTCCTTCTTTTCCTCTGAATCCGGATAAGGCGGATTGGAGTGTATGGGATTTTCCGGATGTTGTGGATCATTGGAATTTCCCGGGATATGAGGGGAAAAAGATGACAGTATCTGTATACTCCAATTGTGAACAGGTTGAACTGTTCTTGAATGGGGAATCTTTAGGAAAACAAGAAAATACTGCCGATAAGAAAAATACGCTTGTCTGGGAAGTACCTTATGCTCATGGAATATTGAAAGCCGTAAGTTATAATAAAGGCGGTGAAGTGGGCACTGCAACGTTGGAAAGTGCTGGTAAGGTTGAAAAGATCAGATTATCTGCGGACAGAACGGAAATCGTAGCTGATGGTAATGATCTAAGCTATATCACATTAGAATTGGTAGATAGTAAAGGCATTAGAAATCAGTTGGCTGAAGAATTGGTAGCATTTTCTATAGAAGGAGATGCTACG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,846 total letters Query= APC115077.119 Length=2547 Score E Sequences producing significant alignments: (Bits) Value wt 3855 0.0 11D_D5_APC115077.119_D05.seq 1535 0.0 05D_D5_APC115077.119_D05.seq 1420 0.0 R E Q S F D E A W L F H R G D I A E G E K Q S L D D S Q W R Query_1 202 CGGGAACAATCTTTCGATGAGGCATGGTTATTTCATCGTGGAGATATTGCCGAAGGAGAAAAGCAATCTTTAGATGACTCACAATGGCGT 291 2 1 .......................................................................................... 90 Q I N L P H D W S I E D I P G T N S P F T A D A A T E V A G Query_1 292 CAGATAAATCTTCCTCATGATTGGAGTATTGAAGATATTCCTGGAACCAATTCTCCTTTTACAGCGGATGCTGCAACGGAAGTTGCAGGT 381 2 91 .......................................................................................... 180 G F T V G G T G W Y R K H F Y I D A A E K G K C I A V S F D Query_1 382 GGTTTTACTGTAGGTGGTACGGGATGGTATAGAAAGCACTTCTACATAGATGCGGCTGAAAAAGGTAAATGTATTGCTGTCTCTTTCGAT 471 2 181 .......................................................................................... 270 G I Y M N A D I W V N D R H V A N H V Y G Y T A F E L D I T Query_1 472 GGAATTTATATGAATGCAGATATCTGGGTGAATGATCGCCATGTAGCCAATCATGTTTATGGATATACTGCATTTGAACTGGATATAACC 561 2 271 .......................................................................................... 360 D Y V R F G A E N L I A V R V K N E G M N C R W Y T G S G Query_1 562 GATTATGTACGTTTCGGAGCTGAAAATCTGATAGCTGTCCGTGTGAAGAATGAA-GGTATGAATTGCCG-TTGGTATACAGGTTC-GGGT 648 2 361 ......................................................-..............-...............-.... 447 1 1143 .....N......AA..A...NNA........N......NT.......N.... 1092 I Y R H T F L K I T N P L H F E T W G T F V T T P Query_1 649 ATT-TACAGGCATACTTTCTT--GAAGA-TAACCAATCCGC--TTCA-TTTTGAAA---CTTGGGG-AACGTTTGT-CACG-ACTCCC-- 723 2 448 ...-.................--.....-............--....-........---.......-.........-....-......-- 522 2 532 ..-- 531 1 1091 N..CN.A..NNN...N.....TNN.N..N..........C.NT....N........ANC..G....G.........T....G......CC 1002 V A T A D K A E V H V Q S V L A N T E K V T G K V Query_1 724 GTTGC--AACG-GCGGATAAAG-CAGAGGTAC--ATGTA--CAGAGTGTTCTGGC--AAATACTGAAAAAG--TAACCGGAAAA-GTGAT 800 2 523 .....--....-..........-.........--.....--..............--..............--...........-..... 599 2 530 .....--....-. 521 1 1001 ...NNCA....C.N..N...N.N........NCNNN...ACNG.N.N.......NCN...N.........AGT..N...A....A.N.N. 912 I L E T R I V D K N N H T V A R K E Q L V T L D N K E K Query_1 801 TC-TGGAAACG-CGGATTGTAG-ATAAGAATAACCATAC-TGTA-GCTCGGAAA--GAGCAACTGGTAAC-ATTGGATAACAAAGAAAAA 882 2 600 ..-........-..........-................-....-.........--..............-................... 681 1 911 ..C.......NT.......N..G................C..NNN......G..AG..............CN.........N...G.... 822 T E V G H A L E V L A P Q L W S I D N P Y L Y Q V V N R L Query_1 883 AC-AGAGGTTGGCCATGCGTTGGAAGTGCTTGCTCCGCAATTATGGTCTATAGACAATCCTTACTTATATCAGGTTGTAAACCGTCTTCT 971 2 682 ..-....................................................................................... 770 1 821 ..C.....................................N.......N...N........N........................N... 732 L Q D D K V I D E E Y I S I G I R N I A F S A E N G F Q L Query_1 972 -GCAAGATGATAAAGTTATAGATGAGG-AATATATTTC-AATAGGTATACGCAATATTGCATTTAGTGCGGAGAATGGTTTCCAGCTGAA 1058 2 771 -..........................-..........-................................................... 857 1 731 T..........................G..........C................................................... 642 N G K S M K L K G G C I H H D N G L L G A K A F D R A E E R Query_1 1059 TGGTAAATCCATGAAACTAAAAGGCGGATGTATCCATCATGACAATGGTCTTTTGGGTGCAAAGGCTTTTGACCGGGCAGAGGAAAGGAA 1148 2 858 .......................................................................................... 947 1 641 .......................................................................................... 552 K I E L L K A A G F N A L R L S H N P P S I A L L N A C D R Query_1 1149 AATAGAACTACTGAAAGCGGCTGGTTTCAATGCGCTGCGCTTGTCTCATAATCCTCCCTCAATCGCTTTACTCAATGCCTGCGACCGCTT 1238 2 948 .......................................................................................... 1037 1 551 .......................................................................................... 462 L G M L V I D E A F D M W R Y G H Y Q Y D Y A Q Y F D K L W Query_1 1239 AGGTATGCTGGTCATAGATGAGGCTTTTGATATGTGGCGCTATGGTCATTATCAGTATGATTATGCACAATACTTTGATAAATTGTGGAA 1328 2 1038 .......................................................................................... 1127 1 461 .......................................................................................... 372 0 8 ......... 16 K E D L H S M V A R D R N H P S V I M W S I G N E I K N K E Query_1 1329 AGAAGATTTGCATAGTATGGTTGCACGGGATAGGAATCATCCTAGTGTTATCATGTGGAGTATTGGTAATGAAATCAAGAACAAAGAAAC 1418 2 1128 .......................................................................................... 1217 1 371 .......................................................................................... 282 0 17 -..............................N.......................................................... 105 T A E I V D I C R E L T G F V K T L D T T R P V T A G V N S Query_1 1419 TGCTGAAATTGTGGATATATGCAGGGAGTTGACAGGTTTTGTGAAGACGCTTGATACAACGCGGCCTGTTACGGCGGGAGTTAATTCTAT 1508 2 1218 .......................................................................................... 1307 1 281 .......................................................................................... 192 0 106 .......................................................................................... 195 I V D A T D D F L A P L D V C G Y N F C L N R Y E S D A K R Query_1 1509 TGTTGATGCAACGGATGATTTTCTGGCTCCTCTGGATGTTTGTGGTTATAATTTTTGTTTAAACCGTTATGAATCGGATGCCAAACGTCA 1598 2 1308 .....................................................AC................................... 1397 1 191 .......................................................................................... 102 0 196 .......................................................................................... 285 H P D R I I Y A S E S Y A S Q A Y D Y W K G V E D H S W V I Query_1 1599 TCCGGACCGTATTATCTATGCTTCGGAGTCCTACGCATCCCAGGCTTATGATTATTGGAAAGGAGTAGAAGATCATTCATGGGTGATCGG 1688 2 1398 .......................................................................................... 1487 1 101 .................................................A....-................................... 13 0 286 .................................................A........................................ 375 G D F I W T A F D Y I G E A S I G W C G Y P L D K R I F P W Query_1 1689 TGATTTTATCTGGACTGCTTTTGACTATATTGGTGAGGCAAGTATCGGCTGGTGTGGGTATCCGCTTGATAAACGTATTTTCCCTTGGAA 1778 2 1488 .......................................................................................... 1577 1 12 -.... 9 0 376 .......................................................................................... 465 N H A N C G D L N L S G E R R P Q S Y L R E T L W S D A P V Query_1 1779 TCATGCCAATTGTGGTGATTTGAATCTTTCGGGCGAACGTCGTCCCCAGTCCTATTTGCGTGAAACGTTATGGAGTGATGCACCGGTATC 1868 2 1578 .......................................................................................... 1667 0 466 ...........................................................................N.............. 555 S H I V V T P P V P S F P L N P D K A D W S V W D F P D V V Query_1 1869 CCATATTGTTGTGACGCCTCCTGTTCCTTCTTTTCCTCTGAATCCGGATAAGGCGGATTGGAGTGTATGGGATTTTCCGGATGTTGTGGA 1958 2 1668 .......................................................................................... 1757 0 556 .................................................................N......................N. 645 D H W N F P G Y E G K K M T V S V Y S N C E Q V E L F L N G Query_1 1959 TCATTGGAATTTCCC-GGGATATGAGGGGAAAAAGATGACAGTATCTGTATACTCCAATTGTGAACAGGTTGAACTGTTCTTGAATGGGG 2047 2 1758 ...............-.......................................................................... 1846 0 646 ...............G....N....N................N.....N.A....................................... 735 E S L G K Q E N T A D K K N T L V W E V P Y A H G I L K A Query_1 2048 AATCTTTAGGAAAACAAGAAAAT-ACTGCCGATAAGAAAAATACGCTTGTCTGGG-AAGTACCTTATGCT-CATGGAATATT-GAAAGCC 2133 2 1847 .......................-...............................-..............-...........-....... 1932 0 736 ......N.N........N.....NN.....N..........N..N..........A..............C...........G....... 825 V S Y N K G G E V G T A T L E S A G K V E K I R L S A Query_1 2134 --GTAAGTTATAATAAAGGC--GGTGAAGTGGGCA-CTGCAACGTTGG--AAAGTGCTGGT-AAGGTTG-AAAAGATCAGATTATCTGCG 2214 2 1933 --..................--.............-............--...........-.......-.................... 2013 0 826 CGT.................NN.TN...N.....NC.NN..C....N.NA....C...N..A.......A....N.........N.N..C 915 D R T E I V A D G N D L S Y I T L E L V D S K G I R N Q L Query_1 2215 GACAGAA-CGGAAAT-CGT-AGCTGATGGTAATGATCTAAGCTATATCACATTAGAATTGGTAGATAGTAAAGGCATTAGAAATCAGTTG 2301 2 2014 .......-.......-...-...................................................................... 2100 0 916 .NNN...T...N...C...A.C..A....C..... 950 A E E L V A F S I E G D A T * H W Query_1 2302 GCTGAAGAATTGGTAGCATTTTCTATAGAAGGAGATGCTACGTAACATTGGA 2353 2 2101 .......................................... 2142 2 658 ........... 668 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12131350 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 4,846 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115086.107 (APC115086.29-766.P(766).D291A.pMCSG68)
KSPVDMDRFIDDLMKKMTLEEKIGQLNLPVTGEITTGQAKSSNVAKRIRAGEVGGLFNLKGVERIRDVQKQAVEESRLGIPLLFGMDVIHGYETVFPIPLGLSCTWNMTAIEESARIAAIEASADGICWTFSPMVDVSRDPRWGRVSEGNGEDPFLGAEIARAMVRGYQGKDMSSNDEIMACVKHFALYGASEAGRDYNTVDMSHQRMFNEYMLPYQAAVEEGVGSVMASFNEVDGVPATGNKWLMTDVLRKQWNFDGFVVTDYTGITEMTDHGMGDTQTVAALALNAGVDMDMVSDAFTSTLKKSLEEGKVSVKAVDAACRRILEAKYKLGLFDNPYKYCDITRPKKQIFTKEHRAIARKTASESFVLLKNENSVLPLAKKGTIAVVGPLADSRSNMPGTWSVAAVMNKYPSLIEGLKEVVGGKAKILTAKGSNLMSDAEYEERATMFGRTLHRDNRTDKELLDEALAVAAKSDVIVAALGESSEMSGESSCRTDLEMPDTQRVLLQELLKTGKPVVLVLFTGRPLVLNWEQENVPAILNVWFGGSEAALAIGDVLFGNVNPSGKLTTTFPKSVGQIPLFYNHKNTGRPLPQGAWFQKFRSNYLDVDNEPLYPFGYGLSYTTFSYSDITLDKSSMNINGEIMATVTVTNTGKYDGSEVVQLYIRDLIGSVTRPVKELKGFEKIFLKAGESKQVSFKLTADMLKFYNYNLDFVCEPGDFEVMIGGDSRDVNKALFSLQ
AAATCTCCTGTCGATATGGATCGCTTTATTGATGATCTGATGAAGAAGATGACTCTGGAAGAGAAAATCGGCCAGTTGAACTTGCCTGTTACGGGTGAAATAACCACCGGACAAGCCAAGAGTAGTAATGTGGCTAAGCGTATCCGTGCCGGTGAAGTGGGCGGACTCTTTAACTTGAAAGGCGTGGAGCGTATTCGTGACGTTCAGAAACAGGCAGTAGAAGAAAGTCGTCTGGGTATTCCTCTTTTATTTGGTATGGATGTAATTCATGGATACGAAACGGTATTTCCTATTCCTCTGGGATTATCCTGTACCTGGAACATGACAGCTATTGAAGAATCTGCACGTATTGCTGCTATCGAAGCCAGTGCTGATGGTATTTGCTGGACATTCAGTCCGATGGTGGATGTTTCCCGTGATCCCCGTTGGGGACGAGTTTCCGAAGGGAATGGTGAAGATCCCTTCTTGGGAGCGGAGATTGCGCGTGCTATGGTACGTGGTTATCAAGGGAAAGATATGAGTAGTAATGATGAAATTATGGCTTGCGTGAAGCACTTTGCGTTATATGGGGCATCAGAAGCCGGACGCGACTATAATACAGTGGATATGAGTCATCAACGTATGTTCAACGAATATATGTTACCTTATCAGGCTGCCGTGGAAGAAGGTGTGGGTAGTGTGATGGCTTCATTCAATGAAGTGGATGGTGTACCGGCTACCGGAAATAAGTGGCTGATGACCGATGTACTTCGTAAGCAGTGGAATTTTGATGGGTTCGTTGTGACGGACTATACCGGTATCACTGAAATGACCGATCATGGTATGGGTGATACACAAACAGTTGCAGCCCTGGCTCTGAATGCAGGTGTCGATATGGATATGGTGAGCGATGCTTTTACAAGCACACTTAAAAAATCTCTGGAAGAAGGAAAAGTTTCAGTAAAGGCTGTTGATGCTGCTTGTCGCCGTATTCTGGAAGCTAAGTATAAGCTGGGGCTTTTTGATAATCCCTATAAATATTGTGATATAACCCGTCCTAAAAAACAAATCTTTACAAAAGAACACCGCGCTATAGCCCGTAAGACAGCTTCGGAAAGCTTTGTTCTCTTGAAGAATGAGAATAGTGTACTCCCTCTGGCAAAGAAAGGTACCATTGCTGTAGTAGGTCCTTTGGCCGATAGCCGTAGCAATATGCCGGGCACGTGGAGTGTGGCCGCTGTGATGAACAAATATCCTTCTTTGATTGAAGGCTTGAAAGAAGTAGTGGGAGGCAAGGCTAAAATTCTTACGGCTAAAGGAAGTAATCTGATGAGTGATGCCGAATACGAAGAACGTGCTACTATGTTTGGCCGTACTCTGCATCGTGACAATCGTACAGATAAGGAACTGCTGGATGAGGCGCTTGCTGTAGCTGCCAAGTCTGACGTGATTGTTGCTGCTTTGGGTGAGTCTTCCGAGATGAGCGGTGAAAGTAGTTGCCGTACAGACCTCGAAATGCCGGATACGCAACGTGTACTTTTGCAGGAATTGTTGAAAACCGGCAAACCGGTGGTATTGGTGTTGTTTACCGGTCGTCCGTTAGTATTGAATTGGGAGCAGGAAAATGTACCTGCTATTCTGAATGTGTGGTTTGGTGGTAGTGAAGCTGCTCTTGCCATTGGTGATGTACTGTTTGGAAATGTAAATCCGAGTGGCAAACTTACTACTACTTTTCCGAAGAGTGTAGGACAGATTCCTTTGTTCTATAACCATAAGAATACTGGTCGTCCTTTGCCTCAAGGGGCCTGGTTCCAGAAGTTCCGTAGCAATTATCTGGATGTAGATAACGAACCGCTTTATCCGTTTGGATATGGCTTGAGCTATACTACTTTCTCTTATAGTGATATTACATTGGATAAATCGTCCATGAATATCAATGGAGAGATTATGGCAACTGTAACGGTAACCAATACAGGTAAGTATGACGGTTCGGAAGTAGTGCAGCTATATATCCGCGATCTTATAGGCAGTGTAACACGTCCGGTGAAAGAACTGAAAGGCTTTGAAAAAATCTTCTTGAAAGCCGGTGAATCCAAACAAGTGTCTTTCAAGTTAACAGCTGATATGTTGAAGTTCTACAATTACAATCTGGATTTTGTGTGCGAACCGGGTGACTTTGAAGTAATGATAGGTGGTGATAGCCGTGATGTGAATAAGGCCTTATTTTCGCTTCAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,103 total letters Query= APC115086.107 Length=2619 Score E Sequences producing significant alignments: (Bits) Value wt 3984 0.0 11E_E5_APC115086.107_E05.seq 1171 0.0 05E_E5_APC115086.107_E05.seq 316 5e-89 K S P V D M D R F I D D L M K K M T L E E K I G Q L N L Query_1 202 AAATCTCCTGTCGATATGGATCGCTTTATTGATGATCTGATGAAGAAGATGACTCTGGAAGAGAAAAT-CGGCC-AGTT-GAAC-TTGCC 287 2 1 ....................................................................-.....-....-....-..... 86 2 867 ............. 879 1 901 .......N..N..T.N...N....N...NN..N.T 867 1 59 ............. 47 P V T G E I T T G Q A K S S N V A K R I R A G E V G Query_1 288 TGTTA-CGGGTGAAA-TAACCA-CCGGAC--AAGCCAAGAG-TAGTAA-TGTGGCTA-AGCG-TAT--CCGTGCCGGTGAA-GTGGGC-- 363 2 87 .....-.........-......-......--..........-......-........-....-...--.............-......-- 162 2 519 ...-......-.. 529 1 866 .....G..N..N...A.....TG.NN.N.CN.NA.......N......A..NN....N....N...TC..T....A.....AN..N..TC 777 1 408 ...-......-.. 398 G L F N L K G V E R I R D V Q K Q A V E E S R L G I Query_1 364 GGACTCTTTAA-CTTGAAAGG-CGTGGA-GCGTATTC-GTGA-CGTTCAGAAA-CAGGCAGTAGAAG-AAAGTC-GTCTGGGT-ATTC-C 443 2 163 ...........-.........-......-........-....-..........-.............-......-........-....-. 242 2 2095 ....-........ 2084 1 776 .A......N..A..A......G......A.....N..C..A.N.T........A.....N.....N.N......C........N....N. 687 P L L F G M D V I H G Y E T V F P I P L G L S C T W N M T A Query_1 444 TCTTTTATTTGG-TATGGATGTAATTCATGGATACGAAACGGTATTTCCTATTCCTCTGGGATTATCCTGTACCTGGAACATGACAGCTA 532 2 243 ............-............................................................................. 331 1 686 .N.....N....G.......T.......NN........................................G................... 597 I E E S A R I A A I E A S A D G I C W T F S P M V D V S R D Query_1 533 TTGAAGAATCTGCACGTATTGCTGCTATCGAAGCCAGTG-CTGATGGTATTTGCTGGACATTCAGTCCGATGGTGGATGTTTCCCGTGAT 621 2 332 .......................................-.................................................. 420 1 596 ....................NN.......N........NA................N................................. 507 P R W G R V S E G N G E D P F L G A E I A R A M V R G Y Q G Query_1 622 CCCCGTTGGGGACGAGTTTCCGAAGGGAATGGTGAAGATCCCTTCTTGGGAGCGGAGATTGCGCGTGCTATGGTACGTGGTTATCAAGGG 711 2 421 .......................................................................................... 510 1 506 ......................N.....................................N............................. 417 K D M S S N D E I M A C V K H F A L Y G A S E A G R D Y N T Query_1 712 AAAGATATGAGTAGTAATGATGAAATTATGGCTTGCGTGAAGCACTTTGCGTTATATGGGGCATCAGAAGCCGGACGCGACTATAATACA 801 2 511 .......................................................................................... 600 2 120 ........... 130 1 416 ..............................N...............N........................................... 327 V D M S H Q R M F N E Y M L P Y Q A A V E E G V G S V M A S Query_1 802 GTGGATATGAGTCATCAACGTATGTTCAACGAATATATGTTACCTTATCAGGCTGCCGTGGAAGAAGGTGTGGGTAGTGTGATGGCTTCA 891 2 601 .......................................................................................... 690 1 326 .......................................................................................... 237 0 9 ........N.......ANN-. 28 F N E V D G V P A T G N K W L M T D V L R K Q W N F D G F Query_1 892 TTCAATGAAGTGGATGGTGTACCGGCTACCGGAAATAAGTGGCTGATGACCGATGTACTTCGTAAGCAGTGGAATT-TTGATGGGTTCGT 980 2 691 ............................................................................-............. 779 1 236 ............................................................................-............. 148 0 29 ............................................................................C............. 118 V V T A Y T G I T E M T D H G M G D T Q T V A A L A L N A Query_1 981 TGTGACGGCTTATACCGGTA-TCAC-TGAAATGACCGATCATGGTATGGGTGATACACAAACAGTTGCAGCCCTGGCTCTGAAT-GCAGG 1067 2 780 ........AC..........-....-..........................................................-..... 866 1 147 ....................-....-.........................................................NAA.... 60 0 119 ...................NC..N.N...............N......................N..N...N.N.N........-.NNN. 207 G V D M D M V S D A F T S T L K K S L E E G K V S V K A V D Query_1 1068 TGTCGATATGGATATGGTGAGCGATGCTTTTACAAGCACACTTAAAAAATCTCTGGAAGAAGGAAAAGTTTCAGTAAAGGCTGTTGATGC 1157 2 867 .......................................................................................... 956 2 9 ............. 21 1 59 .........................N.-.............. 19 0 208 N.....N...A.N....N.N....N.NAN......NTNN.GN...C..CN.C....N.N.........NN.NN.GN.G...C.N..N... 297 A A C R R I L E A K Y K L G L F D N P Y K Y C D I T R P K K Query_1 1158 TGCTTGTCGCCGTATTCTGGAAGCTAAGTATAAGCTGGGGCTTTTTGATAATCCCTATAAATATTGTGATATAACCCGTCCTAAAAAACA 1247 2 957 .......................................................................................... 1046 0 298 ... 300 Q I F T K E H R A I A R K T A S E S F V L L K N E N S V L P Query_1 1248 AATCTTTACAAAAGAACACCGCGCTATAGCCCGTAAGACAGCTTCGGAAAGCTTTGTTCTCTTGAAGAATGAGAATAGTGTACTCCCTCT 1337 2 1047 .......................................................................................... 1136 L A K K G T I A V V G P L A D S R S N M P G T W S V A A V M Query_1 1338 GGCAAAGAAAGGTACCATTGCTGTAGTAGGTCCTTTGGCCGATAGCCGTAGCAATATGCCGGGCACGTGGAGTGTGGCCGCTGTGATGAA 1427 2 1137 .......................................................................................... 1226 N K Y P S L I E G L K E V V G G K A K I L T A K G S N L M S Query_1 1428 CAAATATCCTTCTTTGATTGAAGGCTTGAAAGAAGTAGTGGGAGGCAAGGCTAAAATTCTTACGGCTAAAGGAAGTAATCTGATGAGTGA 1517 2 1227 .......................................................................................... 1316 D A E Y E E R A T M F G R T L H R D N R T D K E L L D E A L Query_1 1518 TGCCGAATACGAAGAACGTGCTACTATGTTTGGCCGTACTCTGCATCGTGACAATCGTACAGATAAGGAACTGCTGGATGAGGCGCTTGC 1607 2 1317 .......................................................................................... 1406 A V A A K S D V I V A A L G E S S E M S G E S S C R T D L E Query_1 1608 TGTAGCTGCCAAGTCTGACGTGATTGTTGCTGCTTTGGGTGAGTCTTCCGAGATGAGCGGTGAAAGTAGTTGCCGTACAGACCTCGAAAT 1697 2 1407 .......................................................................................... 1496 M P D T Q R V L L Q E L L K T G K P V V L V L F T G R P L V Query_1 1698 GCCGGATACGCAACGTGTACTTTTGCAGGAATTGTTGAAAACCGGCAAACCGGTGGTATTGGTGTTGTTTACCGGTCGTCCGTTAGTATT 1787 2 1497 .......................................................................................... 1586 L N W E Q E N V P A I L N V W F G G S E A A L A I G D V L F Query_1 1788 GAATTGGGAGCAGGAAAATGTACCTGCTATTCTGAATGTGTGGTTTGGTGGTAGTGAAGCTGCTCTTGCCATTGGTGATGTACTGTTTGG 1877 2 1587 .......................................................................................... 1676 G N V N P S G K L T T T F P K S V G Q I P L F Y N H K N T G Query_1 1878 AAATGTAAATCCGAGTGGCAAACTTACTACTACTTTTCCGAAGAGTGTAGGACAGATTCCTTTGTTCTATAACCATAAGAATACTGGTCG 1967 2 1677 .......................................................................................... 1766 R P L P Q G A W F Q K F R S N Y L D V D N E P L Y P F G Y G Query_1 1968 TCCTTTGCCTCAAGGGGCCTGGTTCCAGAAGTTCCGTAGCAATTATCTGGATGTAGATAACGAACCGCTTTATCCGTTTGGATATGGCTT 2057 2 1767 .......................................................................................... 1856 L S Y T T F S Y S D I T L D K S S M N I N G E I M A T V T V Query_1 2058 GAGCTATACTACTTTCTCTTATAGTGATATTACATTGGATAAATCGTCCATGAATATCAATGGAGAGATTATGGCAACTGTAACGGTAAC 2147 2 1857 .......................................................................................... 1946 T N T G K Y D G S E V V Q L Y I R D L I G S V T R P V K E L Query_1 2148 CAATACAGGTAAGTATGACGGTTCGGAAGTAGTGCAGCTATATATCCGCGATCTTATAGGCAGTGTAACACGTCCGGTGAAAGAACTGAA 2237 2 1947 .......................................................................................... 2036 K G F E K I F L K A G E S K Q V S F K L T A D M L K F Y N Y Query_1 2238 AGGCTTTGAAAAAATCTTCTTGAAAGCCGGTGAATCCAAACAAGTGTCTTTCAAGTTAACAGCTGATATGTTGAAGTTCTACAATTACAA 2327 2 2037 .......................................................................................... 2126 2 181 ............ 170 N L D F V C E P G D F E V M I G G D S R D V N K A L F S L Q Query_1 2328 TCTGGATTTTGTGTGCGAACCGGGTGACTTTGAAGTAATGATAGGTGGTGATAGCCGTGATGTGAATAAGGCCTTATTTTCGCTTCAA 2415 2 2127 ........................................................................................ 2214 2 2204 .............. 2191 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 13145304 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 5,103 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115086.108 (APC115086.29-766.P(766).E518A.pMCSG68)
KSPVDMDRFIDDLMKKMTLEEKIGQLNLPVTGEITTGQAKSSNVAKRIRAGEVGGLFNLKGVERIRDVQKQAVEESRLGIPLLFGMDVIHGYETVFPIPLGLSCTWNMTAIEESARIAAIEASADGICWTFSPMVDVSRDPRWGRVSEGNGEDPFLGAEIARAMVRGYQGKDMSSNDEIMACVKHFALYGASEAGRDYNTVDMSHQRMFNEYMLPYQAAVEEGVGSVMASFNEVDGVPATGNKWLMTDVLRKQWNFDGFVVTDYTGITEMTDHGMGDTQTVAALALNAGVDMDMVSDAFTSTLKKSLEEGKVSVKAVDAACRRILEAKYKLGLFDNPYKYCDITRPKKQIFTKEHRAIARKTASESFVLLKNENSVLPLAKKGTIAVVGPLADSRSNMPGTWSVAAVMNKYPSLIEGLKEVVGGKAKILTAKGSNLMSDAEYEERATMFGRTLHRDNRTDKELLDEALAVAAKSDVIVAALGESSEMSGESSCRTDLEMPDTQRVLLQELLKTGKPVVLVLFTGRPLVLNWEQENVPAILNVWFGGSEAALAIGDVLFGNVNPSGKLTTTFPKSVGQIPLFYNHKNTGRPLPQGAWFQKFRSNYLDVDNEPLYPFGYGLSYTTFSYSDITLDKSSMNINGEIMATVTVTNTGKYDGSEVVQLYIRDLIGSVTRPVKELKGFEKIFLKAGESKQVSFKLTADMLKFYNYNLDFVCEPGDFEVMIGGDSRDVNKALFSLQ
AAATCTCCTGTCGATATGGATCGCTTTATTGATGATCTGATGAAGAAGATGACTCTGGAAGAGAAAATCGGCCAGTTGAACTTGCCTGTTACGGGTGAAATAACCACCGGACAAGCCAAGAGTAGTAATGTGGCTAAGCGTATCCGTGCCGGTGAAGTGGGCGGACTCTTTAACTTGAAAGGCGTGGAGCGTATTCGTGACGTTCAGAAACAGGCAGTAGAAGAAAGTCGTCTGGGTATTCCTCTTTTATTTGGTATGGATGTAATTCATGGATACGAAACGGTATTTCCTATTCCTCTGGGATTATCCTGTACCTGGAACATGACAGCTATTGAAGAATCTGCACGTATTGCTGCTATCGAAGCCAGTGCTGATGGTATTTGCTGGACATTCAGTCCGATGGTGGATGTTTCCCGTGATCCCCGTTGGGGACGAGTTTCCGAAGGGAATGGTGAAGATCCCTTCTTGGGAGCGGAGATTGCGCGTGCTATGGTACGTGGTTATCAAGGGAAAGATATGAGTAGTAATGATGAAATTATGGCTTGCGTGAAGCACTTTGCGTTATATGGGGCATCAGAAGCCGGACGCGACTATAATACAGTGGATATGAGTCATCAACGTATGTTCAACGAATATATGTTACCTTATCAGGCTGCCGTGGAAGAAGGTGTGGGTAGTGTGATGGCTTCATTCAATGAAGTGGATGGTGTACCGGCTACCGGAAATAAGTGGCTGATGACCGATGTACTTCGTAAGCAGTGGAATTTTGATGGGTTCGTTGTGACGGACTATACCGGTATCACTGAAATGACCGATCATGGTATGGGTGATACACAAACAGTTGCAGCCCTGGCTCTGAATGCAGGTGTCGATATGGATATGGTGAGCGATGCTTTTACAAGCACACTTAAAAAATCTCTGGAAGAAGGAAAAGTTTCAGTAAAGGCTGTTGATGCTGCTTGTCGCCGTATTCTGGAAGCTAAGTATAAGCTGGGGCTTTTTGATAATCCCTATAAATATTGTGATATAACCCGTCCTAAAAAACAAATCTTTACAAAAGAACACCGCGCTATAGCCCGTAAGACAGCTTCGGAAAGCTTTGTTCTCTTGAAGAATGAGAATAGTGTACTCCCTCTGGCAAAGAAAGGTACCATTGCTGTAGTAGGTCCTTTGGCCGATAGCCGTAGCAATATGCCGGGCACGTGGAGTGTGGCCGCTGTGATGAACAAATATCCTTCTTTGATTGAAGGCTTGAAAGAAGTAGTGGGAGGCAAGGCTAAAATTCTTACGGCTAAAGGAAGTAATCTGATGAGTGATGCCGAATACGAAGAACGTGCTACTATGTTTGGCCGTACTCTGCATCGTGACAATCGTACAGATAAGGAACTGCTGGATGAGGCGCTTGCTGTAGCTGCCAAGTCTGACGTGATTGTTGCTGCTTTGGGTGAGTCTTCCGAGATGAGCGGTGAAAGTAGTTGCCGTACAGACCTCGAAATGCCGGATACGCAACGTGTACTTTTGCAGGAATTGTTGAAAACCGGCAAACCGGTGGTATTGGTGTTGTTTACCGGTCGTCCGTTAGTATTGAATTGGGAGCAGGAAAATGTACCTGCTATTCTGAATGTGTGGTTTGGTGGTAGTGAAGCTGCTCTTGCCATTGGTGATGTACTGTTTGGAAATGTAAATCCGAGTGGCAAACTTACTACTACTTTTCCGAAGAGTGTAGGACAGATTCCTTTGTTCTATAACCATAAGAATACTGGTCGTCCTTTGCCTCAAGGGGCCTGGTTCCAGAAGTTCCGTAGCAATTATCTGGATGTAGATAACGAACCGCTTTATCCGTTTGGATATGGCTTGAGCTATACTACTTTCTCTTATAGTGATATTACATTGGATAAATCGTCCATGAATATCAATGGAGAGATTATGGCAACTGTAACGGTAACCAATACAGGTAAGTATGACGGTTCGGAAGTAGTGCAGCTATATATCCGCGATCTTATAGGCAGTGTAACACGTCCGGTGAAAGAACTGAAAGGCTTTGAAAAAATCTTCTTGAAAGCCGGTGAATCCAAACAAGTGTCTTTCAAGTTAACAGCTGATATGTTGAAGTTCTACAATTACAATCTGGATTTTGTGTGCGAACCGGGTGACTTTGAAGTAATGATAGGTGGTGATAGCCGTGATGTGAATAAGGCCTTATTTTCGCTTCAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,973 total letters Query= APC115086.108 Length=2619 Score E Sequences producing significant alignments: (Bits) Value wt 3984 0.0 11F_F5_APC115086.108_F05.seq 1362 0.0 05F_F5_APC115086.108_F05.seq 1220 0.0 K S P V D M D R F I D D L M K K M T L E E K I G Q L N L P V Query_1 202 AAATCTCCTGTCGATATGGATCGCTTTATTGATGATCTGATGAAGAAGATGACTCTGGAAGAGAAAATCGGCCAGTTGAACTTGCCTGTT 291 2 1 .......................................................................................... 90 2 867 ............. 879 1 696 ............ 685 T G E I T T G Q A K S S N V A K R I R A G E V G G L F N L K Query_1 292 ACGGGTGAAATAACCACCGGACAAGCCAAGAGTAGTAATGTGGCTAAGCGTATCCGTGCCGGTGAAGTGGGCGGACTCTTTAACTTGAAA 381 2 91 .......................................................................................... 180 2 2095 ........... 2085 2 519 ........... 529 G V E R I R D V Q K Q A V E E S R L G I P L L F G M D V I H Query_1 382 GGCGTGGAGCGTATTCGTGACGTTCAGAAACAGGCAGTAGAAGAAAGTCGTCTGGGTATTCCTCTTTTATTTGGTATGGATGTAATTCAT 471 2 181 .......................................................................................... 270 2 2084 . 2084 G Y E T V F P I P L G L S C T W N M T A I E E S A R I A A I Query_1 472 GGATACGAAACGGTATTTCCTATTCCTCTGGGATTATCCTGTACCTGGAACATGACAGCTATTGAAGAATCTGCACGTATTGCTGCTATC 561 2 271 .......................................................................................... 360 E A S A D G I C W T F S P M V D V S R D P R W G R V S E G N Query_1 562 GAAGCCAGTGCTGATGGTATTTGCTGGACATTCAGTCCGATGGTGGATGTTTCCCGTGATCCCCGTTGGGGACGAGTTTCCGAAGGGAAT 651 2 361 .......................................................................................... 450 G E D P F L G A E I A R A M V R G Y Q G K D M S S N D E I M Query_1 652 GGTGAAGATCCCTTCTTGGGAGCGGAGATTGCGCGTGCTATGGTACGTGGTTATCAAGGGAAAGATATGAGTAGTAATGATGAAATTATG 741 2 451 .......................................................................................... 540 2 120 ........... 130 A C V K H F A L Y G A S E A G R D Y N T V D M S H Q R M F N Query_1 742 GCTTGCGTGAAGCACTTTGCGTTATATGGGGCATCAGAAGCCGGACGCGACTATAATACAGTGGATATGAGTCATCAACGTATGTTCAAC 831 2 541 .......................................................................................... 630 E Y M L P Y Q A A V E E G V G S V M A S F N E V D G V Query_1 832 GAATATATGTTACCTTATCAGGCTGCCGTGGAAG--AAGGTGTGGG--TAGTG-TGATGG-CTTCATTC-AATG-AAGTGGATGGT-GTA 912 2 631 ..................................--..........--.....-......-........-....-...........-... 711 1 929 ...G.....GA.N...T....GTN....N......G....C...C....N...........T..N 865 P A T G N K W L M T D V L R K Q W N F D G F V V T D Y Query_1 913 CC--GGCTA--CCGGAAATAAGTGGCTG-ATGACC-GATGTACTTCGTAA-GCAGTGGAATTTTGATGGG--TTCGTTGTGACGGACTAT 993 2 712 ..--.....--.................-......-..............-...................--.................. 792 1 864 ..CG.N..TTC.N.......N.......G.N....N..............AN........N......T..NT.................. 775 T G I T E M T D H G M G D T Q T V A A L A L N A G V D M Query_1 994 ACC-GGTATCAC--TGAAATGACCGATCATGGTATGGGTGATACACAAACAGTTGCAGCCC-TGGCTCTGAATGCAGGTGTCGATATGGA 1079 2 793 ...-........--...............................................-............................ 878 2 9 ............ 20 1 774 T..C.....TC.CT..............................N...N.......N....C............................ 685 D M V S D A F T S T L K K S L E E G K V S V K A V D A A C R Query_1 1080 -TATGGTGAGCGATGCTTTT-ACAAGCACACTTAAAAAATCTCTGGAAGAAGGAAAAGTTTCAGTAAAGGCTGTTGATGCTGCTTGTCGC 1167 2 879 -...................-..................................................................... 966 2 21 -. 21 1 684 A...................T..........................N.........N................................ 595 R I L E A K Y K L G L F D N P Y K Y C D I T R P K K Q I F T Query_1 1168 CGTATTCTGGAAGCTAAGTATAAGCTGGGGCTTTTTGATAATCCCTATAAATATTGTGATATAACCCGTCCTAAAAAACAAATCTTTACA 1257 2 967 .......................................................................................... 1056 1 594 .......................................................................................... 505 K E H R A I A R K T A S E S F V L L K N E N S V L P L A K K Query_1 1258 AAAGAACACCGCGCTATAGCCCGTAAGACAGCTTCGGAAAGCTTTGTTCTCTTGAAGAATGAGAATAGTGTACTCCCTCTGGCAAAGAAA 1347 2 1057 .......................................................................................... 1146 1 504 .......N.................................................................................. 415 0 4 ............ 15 G T I A V V G P L A D S R S N M P G T W S V A A V M N K Y Query_1 1348 GGTACCATTGCTGTAGTAG-GTCCTTTGGCCGATAGCCG-TAGCAATATGCCGGGCACGTGGAGTGTGGCCGCTGTGATGAACAAATATC 1435 2 1147 ...................-...................-.................................................. 1234 1 414 ...................-...................-.................................................. 327 0 16 ...................C...................N...N.............................................. 105 P S L I E G L K E V V G G K A K I L T A K G S N L M S D A E Query_1 1436 CTTCTTTGATTGAAGGCTTGAAAGAAGTAGTGGGAGGCAAGGCTAAAATTCTTACGGCTAAAGGAAGTAATCTGATGAGTGATGCCGAAT 1525 2 1235 .......................................................................................... 1324 1 326 .......................................................................................... 237 0 106 .......................................................................................... 195 Y E E R A T M F G R T L H R D N R T D K E L L D E A L A V A Query_1 1526 ACGAAGAACGTGCTACTATGTTTGGCCGTACTCTGCATCGTGACAATCGTACAGATAAGGAACTGCTGGATGAGGCGCTTGCTGTAGCTG 1615 2 1325 .......................................................................................... 1414 1 236 .......................................................................................... 147 0 196 .......................................................................................... 285 A K S D V I V A A L G E S S E M S G A S S C R T D L E M P D Query_1 1616 CCAAGTCTGACGTGATTGTTGCTGCTTTGGGTGAGTCTTCCGAGATGAGCGGTGCTAGTAGTTGCCGTACAGACCTCGAAATGCCGGATA 1705 2 1415 ......................................................AA.................................. 1504 1 146 ....................................AN......................................AA..GG........ 57 0 286 .......................................................................................... 375 T Q R V L L Q E L L K T G K P V V L V L F T G R P L V L N Query_1 1706 CGC-AACGTGTACTTTTGCAGGAATTGTTGAAAACCGGCAAA-CCGGTGGTATTGGTGTTGTTTACCGGTCGTCCGTTAG-TATTGAATT 1792 2 1505 ...-......................................-.....................................-......... 1591 1 56 ...A........................GA...T........N............. 1 0 376 ...-......................................-.....................................T......... 463 W E Q E N V P A I L N V W F G G S E A A L A I G D V L F G N Query_1 1793 GGGAGCAGGAAAATGTACCTGCTATTCTGAATGTGTGGTTTGGTGGTAGTGAAGCTGCTCTTGCCATTGGTGATGTACTGTTTGGAAATG 1882 2 1592 .......................................................................................... 1681 0 464 ..............................................N...................................N....... 553 V N P S G K L T T T F P K S V G Q I P L F Y N H K N T G R P Query_1 1883 TAAATCCGAGTGGCAAACTTACTACTACTTTTCCGAAGAGTGTAGGACAGATTCCTTTGTTCTATAACCATAAGAATACTGGTCGTCCTT 1972 2 1682 .......................................................................................... 1771 0 554 .N..........N...............................N...............N...............N.......N....N 643 L P Q G A W F Q K F R S N Y L D V D N E P L Y P F G Y G Query_1 1973 TGCCTCAAGGGGCCTGGTTCCAGAAGTTCCGTAG-CAATTATCTGGATGTAGA-TAACGAACCGCTTT-ATCCGTTT--GGAT-ATGGCT 2056 2 1772 ..................................-..................-..............-........--....-...... 1855 0 644 ................T........N.N.....NC........NN....CN..NN.............N........NG.N..N...... 733 L S Y T T F S Y S D I T L D K S S M N I N G E I M A T V T Query_1 2057 TGAGCTATACTAC-TTTCTCTTA-TAGT-GATATTACATTGGATAAATCGTCCATGAATATCAATGGAGAGATTATGGCAACTGTAACGG 2143 2 1856 .............-.........-....-............................................................. 1942 0 734 .......N.....T....N....NN...C........N......... 780 V T N T G K Y D G S E V V Q L Y I R D L I G S V T R P V K E Query_1 2144 TAACCAATACAGGTAAGTATGACGGTTCGGAAGTAGTGCAGCTATATATCCGCGATCTTATAGGCAGTGTAACACGTCCGGTGAAAGAAC 2233 2 1943 .......................................................................................... 2032 L K G F E K I F L K A G E S K Q V S F K L T A D M L K F Y N Query_1 2234 TGAAAGGCTTTGAAAAAATCTTCTTGAAAGCCGGTGAATCCAAACAAGTGTCTTTCAAGTTAACAGCTGATATGTTGAAGTTCTACAATT 2323 2 2033 .......................................................................................... 2122 2 181 ............ 170 Y N L D F V C E P G D F E V M I G G D S R D V N K A L F S L Query_1 2324 ACAATCTGGATTTTGTGTGCGAACCGGGTGACTTTGAAGTAATGATAGGTGGTGATAGCCGTGATGTGAATAAGGCCTTATTTTCGCTTC 2413 2 2123 .......................................................................................... 2212 2 2204 .............. 2191 Q Query_1 2414 AA 2415 2 2213 .. 2214 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12807044 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 4,973 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115086.109 (APC115086.29-766.P(766).W430A.pMCSG68)
KSPVDMDRFIDDLMKKMTLEEKIGQLNLPVTGEITTGQAKSSNVAKRIRAGEVGGLFNLKGVERIRDVQKQAVEESRLGIPLLFGMDVIHGYETVFPIPLGLSCTWNMTAIEESARIAAIEASADGICWTFSPMVDVSRDPRWGRVSEGNGEDPFLGAEIARAMVRGYQGKDMSSNDEIMACVKHFALYGASEAGRDYNTVDMSHQRMFNEYMLPYQAAVEEGVGSVMASFNEVDGVPATGNKWLMTDVLRKQWNFDGFVVTDYTGITEMTDHGMGDTQTVAALALNAGVDMDMVSDAFTSTLKKSLEEGKVSVKAVDAACRRILEAKYKLGLFDNPYKYCDITRPKKQIFTKEHRAIARKTASESFVLLKNENSVLPLAKKGTIAVVGPLADSRSNMPGTWSVAAVMNKYPSLIEGLKEVVGGKAKILTAKGSNLMSDAEYEERATMFGRTLHRDNRTDKELLDEALAVAAKSDVIVAALGESSEMSGESSCRTDLEMPDTQRVLLQELLKTGKPVVLVLFTGRPLVLNWEQENVPAILNVWFGGSEAALAIGDVLFGNVNPSGKLTTTFPKSVGQIPLFYNHKNTGRPLPQGAWFQKFRSNYLDVDNEPLYPFGYGLSYTTFSYSDITLDKSSMNINGEIMATVTVTNTGKYDGSEVVQLYIRDLIGSVTRPVKELKGFEKIFLKAGESKQVSFKLTADMLKFYNYNLDFVCEPGDFEVMIGGDSRDVNKALFSLQ
AAATCTCCTGTCGATATGGATCGCTTTATTGATGATCTGATGAAGAAGATGACTCTGGAAGAGAAAATCGGCCAGTTGAACTTGCCTGTTACGGGTGAAATAACCACCGGACAAGCCAAGAGTAGTAATGTGGCTAAGCGTATCCGTGCCGGTGAAGTGGGCGGACTCTTTAACTTGAAAGGCGTGGAGCGTATTCGTGACGTTCAGAAACAGGCAGTAGAAGAAAGTCGTCTGGGTATTCCTCTTTTATTTGGTATGGATGTAATTCATGGATACGAAACGGTATTTCCTATTCCTCTGGGATTATCCTGTACCTGGAACATGACAGCTATTGAAGAATCTGCACGTATTGCTGCTATCGAAGCCAGTGCTGATGGTATTTGCTGGACATTCAGTCCGATGGTGGATGTTTCCCGTGATCCCCGTTGGGGACGAGTTTCCGAAGGGAATGGTGAAGATCCCTTCTTGGGAGCGGAGATTGCGCGTGCTATGGTACGTGGTTATCAAGGGAAAGATATGAGTAGTAATGATGAAATTATGGCTTGCGTGAAGCACTTTGCGTTATATGGGGCATCAGAAGCCGGACGCGACTATAATACAGTGGATATGAGTCATCAACGTATGTTCAACGAATATATGTTACCTTATCAGGCTGCCGTGGAAGAAGGTGTGGGTAGTGTGATGGCTTCATTCAATGAAGTGGATGGTGTACCGGCTACCGGAAATAAGTGGCTGATGACCGATGTACTTCGTAAGCAGTGGAATTTTGATGGGTTCGTTGTGACGGACTATACCGGTATCACTGAAATGACCGATCATGGTATGGGTGATACACAAACAGTTGCAGCCCTGGCTCTGAATGCAGGTGTCGATATGGATATGGTGAGCGATGCTTTTACAAGCACACTTAAAAAATCTCTGGAAGAAGGAAAAGTTTCAGTAAAGGCTGTTGATGCTGCTTGTCGCCGTATTCTGGAAGCTAAGTATAAGCTGGGGCTTTTTGATAATCCCTATAAATATTGTGATATAACCCGTCCTAAAAAACAAATCTTTACAAAAGAACACCGCGCTATAGCCCGTAAGACAGCTTCGGAAAGCTTTGTTCTCTTGAAGAATGAGAATAGTGTACTCCCTCTGGCAAAGAAAGGTACCATTGCTGTAGTAGGTCCTTTGGCCGATAGCCGTAGCAATATGCCGGGCACGTGGAGTGTGGCCGCTGTGATGAACAAATATCCTTCTTTGATTGAAGGCTTGAAAGAAGTAGTGGGAGGCAAGGCTAAAATTCTTACGGCTAAAGGAAGTAATCTGATGAGTGATGCCGAATACGAAGAACGTGCTACTATGTTTGGCCGTACTCTGCATCGTGACAATCGTACAGATAAGGAACTGCTGGATGAGGCGCTTGCTGTAGCTGCCAAGTCTGACGTGATTGTTGCTGCTTTGGGTGAGTCTTCCGAGATGAGCGGTGAAAGTAGTTGCCGTACAGACCTCGAAATGCCGGATACGCAACGTGTACTTTTGCAGGAATTGTTGAAAACCGGCAAACCGGTGGTATTGGTGTTGTTTACCGGTCGTCCGTTAGTATTGAATTGGGAGCAGGAAAATGTACCTGCTATTCTGAATGTGTGGTTTGGTGGTAGTGAAGCTGCTCTTGCCATTGGTGATGTACTGTTTGGAAATGTAAATCCGAGTGGCAAACTTACTACTACTTTTCCGAAGAGTGTAGGACAGATTCCTTTGTTCTATAACCATAAGAATACTGGTCGTCCTTTGCCTCAAGGGGCCTGGTTCCAGAAGTTCCGTAGCAATTATCTGGATGTAGATAACGAACCGCTTTATCCGTTTGGATATGGCTTGAGCTATACTACTTTCTCTTATAGTGATATTACATTGGATAAATCGTCCATGAATATCAATGGAGAGATTATGGCAACTGTAACGGTAACCAATACAGGTAAGTATGACGGTTCGGAAGTAGTGCAGCTATATATCCGCGATCTTATAGGCAGTGTAACACGTCCGGTGAAAGAACTGAAAGGCTTTGAAAAAATCTTCTTGAAAGCCGGTGAATCCAAACAAGTGTCTTTCAAGTTAACAGCTGATATGTTGAAGTTCTACAATTACAATCTGGATTTTGTGTGCGAACCGGGTGACTTTGAAGTAATGATAGGTGGTGATAGCCGTGATGTGAATAAGGCCTTATTTTCGCTTCAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,224 total letters Query= APC115086.109 Length=2619 Score E Sequences producing significant alignments: (Bits) Value wt 3979 0.0 K S P V D M D R F I D D L M K K M T L E E K I G Q L N L P V Query_1 202 AAATCTCCTGTCGATATGGATCGCTTTATTGATGATCTGATGAAGAAGATGACTCTGGAAGAGAAAATCGGCCAGTTGAACTTGCCTGTT 291 2 1 .......................................................................................... 90 2 867 ............. 879 T G E I T T G Q A K S S N V A K R I R A G E V G G L F N L K Query_1 292 ACGGGTGAAATAACCACCGGACAAGCCAAGAGTAGTAATGTGGCTAAGCGTATCCGTGCCGGTGAAGTGGGCGGACTCTTTAACTTGAAA 381 2 91 .......................................................................................... 180 2 2095 ........... 2085 2 519 ........... 529 G V E R I R D V Q K Q A V E E S R L G I P L L F G M D V I H Query_1 382 GGCGTGGAGCGTATTCGTGACGTTCAGAAACAGGCAGTAGAAGAAAGTCGTCTGGGTATTCCTCTTTTATTTGGTATGGATGTAATTCAT 471 2 181 .......................................................................................... 270 2 2084 . 2084 G Y E T V F P I P L G L S C T W N M T A I E E S A R I A A I Query_1 472 GGATACGAAACGGTATTTCCTATTCCTCTGGGATTATCCTGTACCTGGAACATGACAGCTATTGAAGAATCTGCACGTATTGCTGCTATC 561 2 271 .......................................................................................... 360 E A S A D G I C W T F S P M V D V S R D P R W G R V S E G N Query_1 562 GAAGCCAGTGCTGATGGTATTTGCTGGACATTCAGTCCGATGGTGGATGTTTCCCGTGATCCCCGTTGGGGACGAGTTTCCGAAGGGAAT 651 2 361 .......................................................................................... 450 G E D P F L G A E I A R A M V R G Y Q G K D M S S N D E I M Query_1 652 GGTGAAGATCCCTTCTTGGGAGCGGAGATTGCGCGTGCTATGGTACGTGGTTATCAAGGGAAAGATATGAGTAGTAATGATGAAATTATG 741 2 451 .......................................................................................... 540 2 120 ........... 130 A C V K H F A L Y G A S E A G R D Y N T V D M S H Q R M F N Query_1 742 GCTTGCGTGAAGCACTTTGCGTTATATGGGGCATCAGAAGCCGGACGCGACTATAATACAGTGGATATGAGTCATCAACGTATGTTCAAC 831 2 541 .......................................................................................... 630 E Y M L P Y Q A A V E E G V G S V M A S F N E V D G V P A T Query_1 832 GAATATATGTTACCTTATCAGGCTGCCGTGGAAGAAGGTGTGGGTAGTGTGATGGCTTCATTCAATGAAGTGGATGGTGTACCGGCTACC 921 2 631 .......................................................................................... 720 G N K W L M T D V L R K Q W N F D G F V V T D Y T G I T E M Query_1 922 GGAAATAAGTGGCTGATGACCGATGTACTTCGTAAGCAGTGGAATTTTGATGGGTTCGTTGTGACGGACTATACCGGTATCACTGAAATG 1011 2 721 .......................................................................................... 810 T D H G M G D T Q T V A A L A L N A G V D M D M V S D A F T Query_1 1012 ACCGATCATGGTATGGGTGATACACAAACAGTTGCAGCCCTGGCTCTGAATGCAGGTGTCGATATGGATATGGTGAGCGATGCTTTTACA 1101 2 811 .......................................................................................... 900 2 9 ............. 21 S T L K K S L E E G K V S V K A V D A A C R R I L E A K Y K Query_1 1102 AGCACACTTAAAAAATCTCTGGAAGAAGGAAAAGTTTCAGTAAAGGCTGTTGATGCTGCTTGTCGCCGTATTCTGGAAGCTAAGTATAAG 1191 2 901 .......................................................................................... 990 L G L F D N P Y K Y C D I T R P K K Q I F T K E H R A I A R Query_1 1192 CTGGGGCTTTTTGATAATCCCTATAAATATTGTGATATAACCCGTCCTAAAAAACAAATCTTTACAAAAGAACACCGCGCTATAGCCCGT 1281 2 991 .......................................................................................... 1080 K T A S E S F V L L K N E N S V L P L A K K G T I A V V G P Query_1 1282 AAGACAGCTTCGGAAAGCTTTGTTCTCTTGAAGAATGAGAATAGTGTACTCCCTCTGGCAAAGAAAGGTACCATTGCTGTAGTAGGTCCT 1371 2 1081 .......................................................................................... 1170 L A D S R S N M P G T A S V A A V M N K Y P S L I E G L K E Query_1 1372 TTGGCCGATAGCCGTAGCAATATGCCGGGCACGGCTAGTGTGGCCGCTGTGATGAACAAATATCCTTCTTTGATTGAAGGCTTGAAAGAA 1461 2 1171 .................................TGG...................................................... 1260 V V G G K A K I L T A K G S N L M S D A E Y E E R A T M F G Query_1 1462 GTAGTGGGAGGCAAGGCTAAAATTCTTACGGCTAAAGGAAGTAATCTGATGAGTGATGCCGAATACGAAGAACGTGCTACTATGTTTGGC 1551 2 1261 .......................................................................................... 1350 R T L H R D N R T D K E L L D E A L A V A A K S D V I V A A Query_1 1552 CGTACTCTGCATCGTGACAATCGTACAGATAAGGAACTGCTGGATGAGGCGCTTGCTGTAGCTGCCAAGTCTGACGTGATTGTTGCTGCT 1641 2 1351 .......................................................................................... 1440 L G E S S E M S G E S S C R T D L E M P D T Q R V L L Q E L Query_1 1642 TTGGGTGAGTCTTCCGAGATGAGCGGTGAAAGTAGTTGCCGTACAGACCTCGAAATGCCGGATACGCAACGTGTACTTTTGCAGGAATTG 1731 2 1441 .......................................................................................... 1530 L K T G K P V V L V L F T G R P L V L N W E Q E N V P A I L Query_1 1732 TTGAAAACCGGCAAACCGGTGGTATTGGTGTTGTTTACCGGTCGTCCGTTAGTATTGAATTGGGAGCAGGAAAATGTACCTGCTATTCTG 1821 2 1531 .......................................................................................... 1620 N V W F G G S E A A L A I G D V L F G N V N P S G K L T T T Query_1 1822 AATGTGTGGTTTGGTGGTAGTGAAGCTGCTCTTGCCATTGGTGATGTACTGTTTGGAAATGTAAATCCGAGTGGCAAACTTACTACTACT 1911 2 1621 .......................................................................................... 1710 F P K S V G Q I P L F Y N H K N T G R P L P Q G A W F Q K F Query_1 1912 TTTCCGAAGAGTGTAGGACAGATTCCTTTGTTCTATAACCATAAGAATACTGGTCGTCCTTTGCCTCAAGGGGCCTGGTTCCAGAAGTTC 2001 2 1711 .......................................................................................... 1800 R S N Y L D V D N E P L Y P F G Y G L S Y T T F S Y S D I T Query_1 2002 CGTAGCAATTATCTGGATGTAGATAACGAACCGCTTTATCCGTTTGGATATGGCTTGAGCTATACTACTTTCTCTTATAGTGATATTACA 2091 2 1801 .......................................................................................... 1890 L D K S S M N I N G E I M A T V T V T N T G K Y D G S E V V Query_1 2092 TTGGATAAATCGTCCATGAATATCAATGGAGAGATTATGGCAACTGTAACGGTAACCAATACAGGTAAGTATGACGGTTCGGAAGTAGTG 2181 2 1891 .......................................................................................... 1980 Q L Y I R D L I G S V T R P V K E L K G F E K I F L K A G E Query_1 2182 CAGCTATATATCCGCGATCTTATAGGCAGTGTAACACGTCCGGTGAAAGAACTGAAAGGCTTTGAAAAAATCTTCTTGAAAGCCGGTGAA 2271 2 1981 .......................................................................................... 2070 S K Q V S F K L T A D M L K F Y N Y N L D F V C E P G D F E Query_1 2272 TCCAAACAAGTGTCTTTCAAGTTAACAGCTGATATGTTGAAGTTCTACAATTACAATCTGGATTTTGTGTGCGAACCGGGTGACTTTGAA 2361 2 2071 .......................................................................................... 2160 2 181 ............ 170 V M I G G D S R D V N K A L F S L Q Query_1 2362 GTAATGATAGGTGGTGATAGCCGTGATGTGAATAAGGCCTTATTTTCGCTTCAA 2415 2 2161 ...................................................... 2214 2 2204 .............. 2191 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5664128 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 2,224 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115086.112 (APC115086.29-766.P(766).R173A.pMCSG68)
KSPVDMDRFIDDLMKKMTLEEKIGQLNLPVTGEITTGQAKSSNVAKRIRAGEVGGLFNLKGVERIRDVQKQAVEESRLGIPLLFGMDVIHGYETVFPIPLGLSCTWNMTAIEESARIAAIEASADGICWTFSPMVDVSRDPRWGRVSEGNGEDPFLGAEIARAMVRGYQGKDMSSNDEIMACVKHFALYGASEAGRDYNTVDMSHQRMFNEYMLPYQAAVEEGVGSVMASFNEVDGVPATGNKWLMTDVLRKQWNFDGFVVTDYTGITEMTDHGMGDTQTVAALALNAGVDMDMVSDAFTSTLKKSLEEGKVSVKAVDAACRRILEAKYKLGLFDNPYKYCDITRPKKQIFTKEHRAIARKTASESFVLLKNENSVLPLAKKGTIAVVGPLADSRSNMPGTWSVAAVMNKYPSLIEGLKEVVGGKAKILTAKGSNLMSDAEYEERATMFGRTLHRDNRTDKELLDEALAVAAKSDVIVAALGESSEMSGESSCRTDLEMPDTQRVLLQELLKTGKPVVLVLFTGRPLVLNWEQENVPAILNVWFGGSEAALAIGDVLFGNVNPSGKLTTTFPKSVGQIPLFYNHKNTGRPLPQGAWFQKFRSNYLDVDNEPLYPFGYGLSYTTFSYSDITLDKSSMNINGEIMATVTVTNTGKYDGSEVVQLYIRDLIGSVTRPVKELKGFEKIFLKAGESKQVSFKLTADMLKFYNYNLDFVCEPGDFEVMIGGDSRDVNKALFSLQ
AAATCTCCTGTCGATATGGATCGCTTTATTGATGATCTGATGAAGAAGATGACTCTGGAAGAGAAAATCGGCCAGTTGAACTTGCCTGTTACGGGTGAAATAACCACCGGACAAGCCAAGAGTAGTAATGTGGCTAAGCGTATCCGTGCCGGTGAAGTGGGCGGACTCTTTAACTTGAAAGGCGTGGAGCGTATTCGTGACGTTCAGAAACAGGCAGTAGAAGAAAGTCGTCTGGGTATTCCTCTTTTATTTGGTATGGATGTAATTCATGGATACGAAACGGTATTTCCTATTCCTCTGGGATTATCCTGTACCTGGAACATGACAGCTATTGAAGAATCTGCACGTATTGCTGCTATCGAAGCCAGTGCTGATGGTATTTGCTGGACATTCAGTCCGATGGTGGATGTTTCCCGTGATCCCCGTTGGGGACGAGTTTCCGAAGGGAATGGTGAAGATCCCTTCTTGGGAGCGGAGATTGCGCGTGCTATGGTACGTGGTTATCAAGGGAAAGATATGAGTAGTAATGATGAAATTATGGCTTGCGTGAAGCACTTTGCGTTATATGGGGCATCAGAAGCCGGACGCGACTATAATACAGTGGATATGAGTCATCAACGTATGTTCAACGAATATATGTTACCTTATCAGGCTGCCGTGGAAGAAGGTGTGGGTAGTGTGATGGCTTCATTCAATGAAGTGGATGGTGTACCGGCTACCGGAAATAAGTGGCTGATGACCGATGTACTTCGTAAGCAGTGGAATTTTGATGGGTTCGTTGTGACGGACTATACCGGTATCACTGAAATGACCGATCATGGTATGGGTGATACACAAACAGTTGCAGCCCTGGCTCTGAATGCAGGTGTCGATATGGATATGGTGAGCGATGCTTTTACAAGCACACTTAAAAAATCTCTGGAAGAAGGAAAAGTTTCAGTAAAGGCTGTTGATGCTGCTTGTCGCCGTATTCTGGAAGCTAAGTATAAGCTGGGGCTTTTTGATAATCCCTATAAATATTGTGATATAACCCGTCCTAAAAAACAAATCTTTACAAAAGAACACCGCGCTATAGCCCGTAAGACAGCTTCGGAAAGCTTTGTTCTCTTGAAGAATGAGAATAGTGTACTCCCTCTGGCAAAGAAAGGTACCATTGCTGTAGTAGGTCCTTTGGCCGATAGCCGTAGCAATATGCCGGGCACGTGGAGTGTGGCCGCTGTGATGAACAAATATCCTTCTTTGATTGAAGGCTTGAAAGAAGTAGTGGGAGGCAAGGCTAAAATTCTTACGGCTAAAGGAAGTAATCTGATGAGTGATGCCGAATACGAAGAACGTGCTACTATGTTTGGCCGTACTCTGCATCGTGACAATCGTACAGATAAGGAACTGCTGGATGAGGCGCTTGCTGTAGCTGCCAAGTCTGACGTGATTGTTGCTGCTTTGGGTGAGTCTTCCGAGATGAGCGGTGAAAGTAGTTGCCGTACAGACCTCGAAATGCCGGATACGCAACGTGTACTTTTGCAGGAATTGTTGAAAACCGGCAAACCGGTGGTATTGGTGTTGTTTACCGGTCGTCCGTTAGTATTGAATTGGGAGCAGGAAAATGTACCTGCTATTCTGAATGTGTGGTTTGGTGGTAGTGAAGCTGCTCTTGCCATTGGTGATGTACTGTTTGGAAATGTAAATCCGAGTGGCAAACTTACTACTACTTTTCCGAAGAGTGTAGGACAGATTCCTTTGTTCTATAACCATAAGAATACTGGTCGTCCTTTGCCTCAAGGGGCCTGGTTCCAGAAGTTCCGTAGCAATTATCTGGATGTAGATAACGAACCGCTTTATCCGTTTGGATATGGCTTGAGCTATACTACTTTCTCTTATAGTGATATTACATTGGATAAATCGTCCATGAATATCAATGGAGAGATTATGGCAACTGTAACGGTAACCAATACAGGTAAGTATGACGGTTCGGAAGTAGTGCAGCTATATATCCGCGATCTTATAGGCAGTGTAACACGTCCGGTGAAAGAACTGAAAGGCTTTGAAAAAATCTTCTTGAAAGCCGGTGAATCCAAACAAGTGTCTTTCAAGTTAACAGCTGATATGTTGAAGTTCTACAATTACAATCTGGATTTTGTGTGCGAACCGGGTGACTTTGAAGTAATGATAGGTGGTGATAGCCGTGATGTGAATAAGGCCTTATTTTCGCTTCAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,604 total letters Query= APC115086.112 Length=2619 Score E Sequences producing significant alignments: (Bits) Value wt 3979 0.0 11H_H5_APC115086.112_H05.seq 883 0.0 K S P V D M D R F I D D L M K K M T L E E K I G Q L N L P Query_1 202 AAATCTCCTGTCGATATGGATCGCTTTATTGATGATCTGATGAAGAAGATGACTCTGGAAGAGAAAATCGGCC-AGTTG-AACTTGCCTG 289 2 1 .........................................................................-.....-.......... 88 2 867 ............. 879 1 581 ...........N..C.N...N.....N.N.. 551 V T G E I T T G Q A K S S N V A K R I R A G E V G G L F N L Query_1 290 TTACGG-GTGAAATAACCACCGGACAAGCCAAGAGTAGTAATGTGGCTAAGCGTATCCGTGCCGGTGAAGTGGGCGGACTCTTTAACTTG 378 2 89 ......-................................................................................... 177 2 2095 ........ 2088 2 519 ........... 529 1 550 ...N..T.NN.................N........N......N...........................T.............N..N. 461 1 118 ........... 108 K G V E R I R D V Q K Q A V E E S R L G I P L L F G M D V I Query_1 379 AAAGGCGTGGAGCGTATTCGTGACGTTCAGAAACAGGCAGTAGAAGAAAGTCGTCTGGGTATTCCTCTTTTATTTGGTATGGATGTAATT 468 2 178 .......................................................................................... 267 2 2087 .... 2084 1 460 .....N.........................................................................N.......... 371 H G Y E T V F P I P L G L S C T W N M T A I E E S A R I A A Query_1 469 CATGGATACGAAACGGTATTTCCTATTCCTCTGGGATTATCCTGTACCTGGAACATGACAGCTATTGAAGAATCTGCACGTATTGCTGCT 558 2 268 .......................................................................................... 357 1 370 .......................................................................................... 281 I E A S A D G I C W T F S P M V D V S R D P R W G A V S E G Query_1 559 ATCGAAGCCAGTGCTGATGGTATTTGCTGGACATTCAGTCCGATGGTGGATGTTTCCCGTGATCCCCGTTGGGGAGCTGTTTCCGAAGGG 648 2 358 ...........................................................................CGA............ 447 1 280 .......................................................................................... 191 N G E D P F L G A E I A R A M V R G Y Q G K D M S S N D E Query_1 649 AATGGTGAAGATCCCTTCTTGGGA-GCGGAGATTGCGCGTGCTATGGTACGTGGTTATCAAGGGAAAGATATGAGTAGTAATGATGAAAT 737 2 448 ........................-................................................................. 536 2 120 ........... 130 1 190 ........................T.........................................N....................... 101 I M A C V K H F A L Y G A S E A G R D Y N T V D M S H Q R Query_1 738 TATGGCTTGCGTGAAGCACTTTGCGTTATATGGGGCATCA-GAAG-CCGGACGCGACTATAATA-CAGTGGATATGAGTCATCAACGTAT 824 2 537 ........................................-....-..................-......................... 623 1 100 ........................................N....N..................N.G.N...N.NT....N.... 16 M F N E Y M L P Y Q A A V E E G V G S V M A S F N E V D G V Query_1 825 GTTCAACGAATATATGTTACCTTATCAGGCTGCCGTGGAAGAAGGTGTGGGTAGTGTGATGGCTTCATTCAATGAAGTGGATGGTGTACC 914 2 624 .......................................................................................... 713 P A T G N K W L M T D V L R K Q W N F D G F V V T D Y T G I Query_1 915 GGCTACCGGAAATAAGTGGCTGATGACCGATGTACTTCGTAAGCAGTGGAATTTTGATGGGTTCGTTGTGACGGACTATACCGGTATCAC 1004 2 714 .......................................................................................... 803 T E M T D H G M G D T Q T V A A L A L N A G V D M D M V S D Query_1 1005 TGAAATGACCGATCATGGTATGGGTGATACACAAACAGTTGCAGCCCTGGCTCTGAATGCAGGTGTCGATATGGATATGGTGAGCGATGC 1094 2 804 .......................................................................................... 893 2 9 ............. 21 A F T S T L K K S L E E G K V S V K A V D A A C R R I L E A Query_1 1095 TTTTACAAGCACACTTAAAAAATCTCTGGAAGAAGGAAAAGTTTCAGTAAAGGCTGTTGATGCTGCTTGTCGCCGTATTCTGGAAGCTAA 1184 2 894 .......................................................................................... 983 K Y K L G L F D N P Y K Y C D I T R P K K Q I F T K E H R A Query_1 1185 GTATAAGCTGGGGCTTTTTGATAATCCCTATAAATATTGTGATATAACCCGTCCTAAAAAACAAATCTTTACAAAAGAACACCGCGCTAT 1274 2 984 .......................................................................................... 1073 I A R K T A S E S F V L L K N E N S V L P L A K K G T I A V Query_1 1275 AGCCCGTAAGACAGCTTCGGAAAGCTTTGTTCTCTTGAAGAATGAGAATAGTGTACTCCCTCTGGCAAAGAAAGGTACCATTGCTGTAGT 1364 2 1074 .......................................................................................... 1163 V G P L A D S R S N M P G T W S V A A V M N K Y P S L I E G Query_1 1365 AGGTCCTTTGGCCGATAGCCGTAGCAATATGCCGGGCACGTGGAGTGTGGCCGCTGTGATGAACAAATATCCTTCTTTGATTGAAGGCTT 1454 2 1164 .......................................................................................... 1253 L K E V V G G K A K I L T A K G S N L M S D A E Y E E R A T Query_1 1455 GAAAGAAGTAGTGGGAGGCAAGGCTAAAATTCTTACGGCTAAAGGAAGTAATCTGATGAGTGATGCCGAATACGAAGAACGTGCTACTAT 1544 2 1254 .......................................................................................... 1343 M F G R T L H R D N R T D K E L L D E A L A V A A K S D V I Query_1 1545 GTTTGGCCGTACTCTGCATCGTGACAATCGTACAGATAAGGAACTGCTGGATGAGGCGCTTGCTGTAGCTGCCAAGTCTGACGTGATTGT 1634 2 1344 .......................................................................................... 1433 V A A L G E S S E M S G E S S C R T D L E M P D T Q R V L L Query_1 1635 TGCTGCTTTGGGTGAGTCTTCCGAGATGAGCGGTGAAAGTAGTTGCCGTACAGACCTCGAAATGCCGGATACGCAACGTGTACTTTTGCA 1724 2 1434 .......................................................................................... 1523 Q E L L K T G K P V V L V L F T G R P L V L N W E Q E N V P Query_1 1725 GGAATTGTTGAAAACCGGCAAACCGGTGGTATTGGTGTTGTTTACCGGTCGTCCGTTAGTATTGAATTGGGAGCAGGAAAATGTACCTGC 1814 2 1524 .......................................................................................... 1613 A I L N V W F G G S E A A L A I G D V L F G N V N P S G K L Query_1 1815 TATTCTGAATGTGTGGTTTGGTGGTAGTGAAGCTGCTCTTGCCATTGGTGATGTACTGTTTGGAAATGTAAATCCGAGTGGCAAACTTAC 1904 2 1614 .......................................................................................... 1703 T T T F P K S V G Q I P L F Y N H K N T G R P L P Q G A W F Query_1 1905 TACTACTTTTCCGAAGAGTGTAGGACAGATTCCTTTGTTCTATAACCATAAGAATACTGGTCGTCCTTTGCCTCAAGGGGCCTGGTTCCA 1994 2 1704 .......................................................................................... 1793 Q K F R S N Y L D V D N E P L Y P F G Y G L S Y T T F S Y S Query_1 1995 GAAGTTCCGTAGCAATTATCTGGATGTAGATAACGAACCGCTTTATCCGTTTGGATATGGCTTGAGCTATACTACTTTCTCTTATAGTGA 2084 2 1794 .......................................................................................... 1883 D I T L D K S S M N I N G E I M A T V T V T N T G K Y D G S Query_1 2085 TATTACATTGGATAAATCGTCCATGAATATCAATGGAGAGATTATGGCAACTGTAACGGTAACCAATACAGGTAAGTATGACGGTTCGGA 2174 2 1884 .......................................................................................... 1973 E V V Q L Y I R D L I G S V T R P V K E L K G F E K I F L K Query_1 2175 AGTAGTGCAGCTATATATCCGCGATCTTATAGGCAGTGTAACACGTCCGGTGAAAGAACTGAAAGGCTTTGAAAAAATCTTCTTGAAAGC 2264 2 1974 .......................................................................................... 2063 A G E S K Q V S F K L T A D M L K F Y N Y N L D F V C E P G Query_1 2265 CGGTGAATCCAAACAAGTGTCTTTCAAGTTAACAGCTGATATGTTGAAGTTCTACAATTACAATCTGGATTTTGTGTGCGAACCGGGTGA 2354 2 2064 .......................................................................................... 2153 2 181 ............ 170 D F E V M I G G D S R D V N K A L F S L Q Query_1 2355 CTTTGAAGTAATGATAGGTGGTGATAGCCGTGATGTGAATAAGGCCTTATTTTCGCTTCAA 2415 2 2154 ............................................................. 2214 2 2204 .............. 2191 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 9244906 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 3,604 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115086.114 (APC115086.29-766.P(766).H213A.pMCSG68)
KSPVDMDRFIDDLMKKMTLEEKIGQLNLPVTGEITTGQAKSSNVAKRIRAGEVGGLFNLKGVERIRDVQKQAVEESRLGIPLLFGMDVIHGYETVFPIPLGLSCTWNMTAIEESARIAAIEASADGICWTFSPMVDVSRDPRWGRVSEGNGEDPFLGAEIARAMVRGYQGKDMSSNDEIMACVKHFALYGASEAGRDYNTVDMSHQRMFNEYMLPYQAAVEEGVGSVMASFNEVDGVPATGNKWLMTDVLRKQWNFDGFVVTDYTGITEMTDHGMGDTQTVAALALNAGVDMDMVSDAFTSTLKKSLEEGKVSVKAVDAACRRILEAKYKLGLFDNPYKYCDITRPKKQIFTKEHRAIARKTASESFVLLKNENSVLPLAKKGTIAVVGPLADSRSNMPGTWSVAAVMNKYPSLIEGLKEVVGGKAKILTAKGSNLMSDAEYEERATMFGRTLHRDNRTDKELLDEALAVAAKSDVIVAALGESSEMSGESSCRTDLEMPDTQRVLLQELLKTGKPVVLVLFTGRPLVLNWEQENVPAILNVWFGGSEAALAIGDVLFGNVNPSGKLTTTFPKSVGQIPLFYNHKNTGRPLPQGAWFQKFRSNYLDVDNEPLYPFGYGLSYTTFSYSDITLDKSSMNINGEIMATVTVTNTGKYDGSEVVQLYIRDLIGSVTRPVKELKGFEKIFLKAGESKQVSFKLTADMLKFYNYNLDFVCEPGDFEVMIGGDSRDVNKALFSLQ
AAATCTCCTGTCGATATGGATCGCTTTATTGATGATCTGATGAAGAAGATGACTCTGGAAGAGAAAATCGGCCAGTTGAACTTGCCTGTTACGGGTGAAATAACCACCGGACAAGCCAAGAGTAGTAATGTGGCTAAGCGTATCCGTGCCGGTGAAGTGGGCGGACTCTTTAACTTGAAAGGCGTGGAGCGTATTCGTGACGTTCAGAAACAGGCAGTAGAAGAAAGTCGTCTGGGTATTCCTCTTTTATTTGGTATGGATGTAATTCATGGATACGAAACGGTATTTCCTATTCCTCTGGGATTATCCTGTACCTGGAACATGACAGCTATTGAAGAATCTGCACGTATTGCTGCTATCGAAGCCAGTGCTGATGGTATTTGCTGGACATTCAGTCCGATGGTGGATGTTTCCCGTGATCCCCGTTGGGGACGAGTTTCCGAAGGGAATGGTGAAGATCCCTTCTTGGGAGCGGAGATTGCGCGTGCTATGGTACGTGGTTATCAAGGGAAAGATATGAGTAGTAATGATGAAATTATGGCTTGCGTGAAGCACTTTGCGTTATATGGGGCATCAGAAGCCGGACGCGACTATAATACAGTGGATATGAGTCATCAACGTATGTTCAACGAATATATGTTACCTTATCAGGCTGCCGTGGAAGAAGGTGTGGGTAGTGTGATGGCTTCATTCAATGAAGTGGATGGTGTACCGGCTACCGGAAATAAGTGGCTGATGACCGATGTACTTCGTAAGCAGTGGAATTTTGATGGGTTCGTTGTGACGGACTATACCGGTATCACTGAAATGACCGATCATGGTATGGGTGATACACAAACAGTTGCAGCCCTGGCTCTGAATGCAGGTGTCGATATGGATATGGTGAGCGATGCTTTTACAAGCACACTTAAAAAATCTCTGGAAGAAGGAAAAGTTTCAGTAAAGGCTGTTGATGCTGCTTGTCGCCGTATTCTGGAAGCTAAGTATAAGCTGGGGCTTTTTGATAATCCCTATAAATATTGTGATATAACCCGTCCTAAAAAACAAATCTTTACAAAAGAACACCGCGCTATAGCCCGTAAGACAGCTTCGGAAAGCTTTGTTCTCTTGAAGAATGAGAATAGTGTACTCCCTCTGGCAAAGAAAGGTACCATTGCTGTAGTAGGTCCTTTGGCCGATAGCCGTAGCAATATGCCGGGCACGTGGAGTGTGGCCGCTGTGATGAACAAATATCCTTCTTTGATTGAAGGCTTGAAAGAAGTAGTGGGAGGCAAGGCTAAAATTCTTACGGCTAAAGGAAGTAATCTGATGAGTGATGCCGAATACGAAGAACGTGCTACTATGTTTGGCCGTACTCTGCATCGTGACAATCGTACAGATAAGGAACTGCTGGATGAGGCGCTTGCTGTAGCTGCCAAGTCTGACGTGATTGTTGCTGCTTTGGGTGAGTCTTCCGAGATGAGCGGTGAAAGTAGTTGCCGTACAGACCTCGAAATGCCGGATACGCAACGTGTACTTTTGCAGGAATTGTTGAAAACCGGCAAACCGGTGGTATTGGTGTTGTTTACCGGTCGTCCGTTAGTATTGAATTGGGAGCAGGAAAATGTACCTGCTATTCTGAATGTGTGGTTTGGTGGTAGTGAAGCTGCTCTTGCCATTGGTGATGTACTGTTTGGAAATGTAAATCCGAGTGGCAAACTTACTACTACTTTTCCGAAGAGTGTAGGACAGATTCCTTTGTTCTATAACCATAAGAATACTGGTCGTCCTTTGCCTCAAGGGGCCTGGTTCCAGAAGTTCCGTAGCAATTATCTGGATGTAGATAACGAACCGCTTTATCCGTTTGGATATGGCTTGAGCTATACTACTTTCTCTTATAGTGATATTACATTGGATAAATCGTCCATGAATATCAATGGAGAGATTATGGCAACTGTAACGGTAACCAATACAGGTAAGTATGACGGTTCGGAAGTAGTGCAGCTATATATCCGCGATCTTATAGGCAGTGTAACACGTCCGGTGAAAGAACTGAAAGGCTTTGAAAAAATCTTCTTGAAAGCCGGTGAATCCAAACAAGTGTCTTTCAAGTTAACAGCTGATATGTTGAAGTTCTACAATTACAATCTGGATTTTGTGTGCGAACCGGGTGACTTTGAAGTAATGATAGGTGGTGATAGCCGTGATGTGAATAAGGCCTTATTTTCGCTTCAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,904 total letters Query= APC115086.114 Length=2619 Score E Sequences producing significant alignments: (Bits) Value wt 3979 0.0 12A_A6_APC115086.114_A06.seq 1296 0.0 06A_A6_APC115086.114_A06.seq 1281 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F Query_1 1 TCTCGAT-CCCGCGAAA--TTAATA-CGAC-TCACTATAGGGG--AATTGTGA-GCGGATAAC-AATTCC-CCTC-TAGAA-ATAATTTT 78 1 855 .....N.N......G..AT.....NT..N.C............GA.N.....NN.........C......N....C.....TN..T.... 766 V * L * E G D I H M H H H H H H S S G V D L W S H P Q F Query_1 79 GTTTAAC-TTTAAGAAGG-AGATATACATATGCACC-ATCAT-CATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGA 164 1 765 .......C....G.....G..............N..C.....G....................N......N................... 676 E K G T E N L Y F Q S N A K S P V D M D R F I D D L M K K M Query_1 165 AAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCAAATCTCCTGTCGATATGGATCGCTTTATTGATGATCTGATGAAGAAGATGAC 254 2 1 ..................................................... 53 2 867 ............. 879 1 675 .......C.................................................................................. 586 0 382 ............. 394 T L E E K I G Q L N L P V T G E I T T G Q A K S S N V A K R Query_1 255 TCTGGAAGAGAAAATCGGCCAGTTGAACTTGCCTGTTACGGGTGAAATAACCACCGGACAAGCCAAGAGTAGTAATGTGGCTAAGCGTAT 344 2 54 .......................................................................................... 143 2 519 ........... 529 1 585 .......................................................................................... 496 1 120 ........... 110 0 34 ........... 44 I R A G E V G G L F N L K G V E R I R D V Q K Q A V E E S R Query_1 345 CCGTGCCGGTGAAGTGGGCGGACTCTTTAACTTGAAAGGCGTGGAGCGTATTCGTGACGTTCAGAAACAGGCAGTAGAAGAAAGTCGTCT 434 2 144 .......................................................................................... 233 2 2095 ............ 2084 1 495 .......................................................................................... 406 L G I P L L F G M D V I H G Y E T V F P I P L G L S C T W N Query_1 435 GGGTATTCCTCTTTTATTTGGTATGGATGTAATTCATGGATACGAAACGGTATTTCCTATTCCTCTGGGATTATCCTGTACCTGGAACAT 524 2 234 .......................................................................................... 323 1 405 .......................................................................................... 316 M T A I E E S A R I A A I E A S A D G I C W T F S P M V D V Query_1 525 GACAGCTATTGAAGAATCTGCACGTATTGCTGCTATCGAAGCCAGTGCTGATGGTATTTGCTGGACATTCAGTCCGATGGTGGATGTTTC 614 2 324 .......................................................................................... 413 1 315 .......................................................................................... 226 S R D P R W G R V S E G N G E D P F L G A E I A R A M V R G Query_1 615 CCGTGATCCCCGTTGGGGACGAGTTTCCGAAGGGAATGGTGAAGATCCCTTCTTGGGAGCGGAGATTGCGCGTGCTATGGTACGTGGTTA 704 2 414 .......................................................................................... 503 1 225 .......................................................................................... 136 0 1 .................. 18 Y Q G K D M S S N D E I M A C V K A F A L Y G A S E A G R Query_1 705 TCAAGGGAAAGATATGAGTAGTAATGATGAAATTATGGCTTGCGTGAAGGCTTTTGCGTTATA-TGGGGCATCAGAAGCCGGAC--GCGA 791 2 504 .................................................CAC...........-....................--.... 590 2 120 ........... 130 1 135 ...........................................................A.N.G....................CG.... 46 1 519 ........... 509 0 19 ...............................................................-....................--.... 105 D Y N T V D M S H Q R M F N E Y M L P Y Q A A V E E G V G S Query_1 792 -CTATAAT-ACAGTGGATATGAGTCATCAACGTATGTTCAACGAATATATGTTACCTTATCAGGCTGCCGTGGAAGAAGGTGTGGGTAGT 879 2 591 -.......-................................................................................. 678 1 45 C.......A..G................N... 14 0 106 -.......-................................................................................. 193 V M A S F N E V D G V P A T G N K W L M T D V L R K Q W N F Query_1 880 GTGATGGCTTCATTCAATGAAGTGGATGGTGTACCGGCTACCGGAAATAAGTGGCTGATGACCGATGTACTTCGTAAGCAGTGGAATTTT 969 2 679 .......................................................................................... 768 0 194 .......................................................................................... 283 D G F V V T D Y T G I T E M T D H G M G D T Q T V A A L A L Query_1 970 GATGGGTTCGTTGTGACGGACTATACCGGTATCACTGAAATGACCGATCATGGTATGGGTGATACACAAACAGTTGCAGCCCTGGCTCTG 1059 2 769 .......................................................................................... 858 0 284 .......................................................................................... 373 N A G V D M D M V S D A F T S T L K K S L E E G K V S V K A Query_1 1060 AATGCAGGTGTCGATATGGATATGGTGAGCGATGCTTTTACAAGCACACTTAAAAAATCTCTGGAAGAAGGAAAAGTTTCAGTAAAGGCT 1149 2 859 .......................................................................................... 948 2 9 ............. 21 1 630 ............. 618 0 374 .............................................................................N............ 463 V D A A C R R I L E A K Y K L G L F D N P Y K Y C D I T R Query_1 1150 GTTGATGCTGCTTGTCGCCGTATTCTGGAAG-CTAAGTATAAGCTGGGGCTTTTTGATAATCCCTATAAATATTGTGATATAACCCGTCC 1238 2 949 ...............................-.......................................................... 1037 0 464 ...............................C...............................................N.......... 553 P K K Q I F T K E H R A I A R K T A S E S F V L L K N E N S Query_1 1239 T-AAAAAACAAATCTTTACAAAAGAACA-CCGCGCTATAGCCCGTAAGACAGCTTCGGAAAGCTTTGTTCTCTTGAAGAATGAGAATAGT 1326 2 1038 .-..........................-............................................................. 1125 0 554 .A..........N..........N....C...........................C....................N..N..N...... 643 V L P L A K K G T I A V V G P L A D S R S N M P G T W S V Query_1 1327 GTACTCCCTCTGGCAAAGAAAGGTACCATTGCTGTAGTAGGTCCTTTGGCCGATAGCCGTAGCAATATGCCGGGCACGTGG-AGT-GTGG 1414 2 1126 .................................................................................-...-.... 1213 0 644 ..................................N....................N..N.........N........N..NT..NC.... 733 A A V M N K Y P S L I E G L K E V V G G K A K I L T A Query_1 1415 -CCGCTGTG-ATGAAC-AAATATCC--TTCTTTGATTGAAGGCTTGAAAGAA-GTAGTGGGAGGCAAGG-CTAAAA--TTCTTACGGCTA 1495 2 1214 -........-......-........--.........................-................-......--............ 1294 0 734 C..N..NN.C......N........CN.C......NC...............CNN..............C......AT.N.CN..C.... 823 K G S N L M S D A E Y E E R A T M F G R T L H R D N R T D K Query_1 1496 AAGGAAGTAATCTGATGAGTGATGCCGAATACGAAGAACGTGCTACTATGTTTGGCCGTACTCTGCATCGTGACAATCGTACAGATAAGG 1585 2 1295 .......................................................................................... 1384 0 824 .. 825 E L L D E A L A V A A K S D V I V A A L G E S S E M S G E S Query_1 1586 AACTGCTGGATGAGGCGCTTGCTGTAGCTGCCAAGTCTGACGTGATTGTTGCTGCTTTGGGTGAGTCTTCCGAGATGAGCGGTGAAAGTA 1675 2 1385 .......................................................................................... 1474 S C R T D L E M P D T Q R V L L Q E L L K T G K P V V L V L Query_1 1676 GTTGCCGTACAGACCTCGAAATGCCGGATACGCAACGTGTACTTTTGCAGGAATTGTTGAAAACCGGCAAACCGGTGGTATTGGTGTTGT 1765 2 1475 .......................................................................................... 1564 F T G R P L V L N W E Q E N V P A I L N V W F G G S E A A L Query_1 1766 TTACCGGTCGTCCGTTAGTATTGAATTGGGAGCAGGAAAATGTACCTGCTATTCTGAATGTGTGGTTTGGTGGTAGTGAAGCTGCTCTTG 1855 2 1565 .......................................................................................... 1654 A I G D V L F G N V N P S G K L T T T F P K S V G Q I P L F Query_1 1856 CCATTGGTGATGTACTGTTTGGAAATGTAAATCCGAGTGGCAAACTTACTACTACTTTTCCGAAGAGTGTAGGACAGATTCCTTTGTTCT 1945 2 1655 .......................................................................................... 1744 Y N H K N T G R P L P Q G A W F Q K F R S N Y L D V D N E P Query_1 1946 ATAACCATAAGAATACTGGTCGTCCTTTGCCTCAAGGGGCCTGGTTCCAGAAGTTCCGTAGCAATTATCTGGATGTAGATAACGAACCGC 2035 2 1745 .......................................................................................... 1834 L Y P F G Y G L S Y T T F S Y S D I T L D K S S M N I N G E Query_1 2036 TTTATCCGTTTGGATATGGCTTGAGCTATACTACTTTCTCTTATAGTGATATTACATTGGATAAATCGTCCATGAATATCAATGGAGAGA 2125 2 1835 .......................................................................................... 1924 I M A T V T V T N T G K Y D G S E V V Q L Y I R D L I G S V Query_1 2126 TTATGGCAACTGTAACGGTAACCAATACAGGTAAGTATGACGGTTCGGAAGTAGTGCAGCTATATATCCGCGATCTTATAGGCAGTGTAA 2215 2 1925 .......................................................................................... 2014 T R P V K E L K G F E K I F L K A G E S K Q V S F K L T A D Query_1 2216 CACGTCCGGTGAAAGAACTGAAAGGCTTTGAAAAAATCTTCTTGAAAGCCGGTGAATCCAAACAAGTGTCTTTCAAGTTAACAGCTGATA 2305 2 2015 .......................................................................................... 2104 2 181 ............ 170 1 458 ............ 469 M L K F Y N Y N L D F V C E P G D F E V M I G G D S R D V N Query_1 2306 TGTTGAAGTTCTACAATTACAATCTGGATTTTGTGTGCGAACCGGGTGACTTTGAAGTAATGATAGGTGGTGATAGCCGTGATGTGAATA 2395 2 2105 .......................................................................................... 2194 2 2204 .... 2201 K A L F S L Q Query_1 2396 AGGCCTTATTTTCGCTTCAA 2415 2 2195 .................... 2214 2 2200 .......... 2191 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12627506 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 4,904 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115086.115 (APC115086.29-766.P(766).F259A.pMCSG68)
KSPVDMDRFIDDLMKKMTLEEKIGQLNLPVTGEITTGQAKSSNVAKRIRAGEVGGLFNLKGVERIRDVQKQAVEESRLGIPLLFGMDVIHGYETVFPIPLGLSCTWNMTAIEESARIAAIEASADGICWTFSPMVDVSRDPRWGRVSEGNGEDPFLGAEIARAMVRGYQGKDMSSNDEIMACVKHFALYGASEAGRDYNTVDMSHQRMFNEYMLPYQAAVEEGVGSVMASFNEVDGVPATGNKWLMTDVLRKQWNFDGFVVTDYTGITEMTDHGMGDTQTVAALALNAGVDMDMVSDAFTSTLKKSLEEGKVSVKAVDAACRRILEAKYKLGLFDNPYKYCDITRPKKQIFTKEHRAIARKTASESFVLLKNENSVLPLAKKGTIAVVGPLADSRSNMPGTWSVAAVMNKYPSLIEGLKEVVGGKAKILTAKGSNLMSDAEYEERATMFGRTLHRDNRTDKELLDEALAVAAKSDVIVAALGESSEMSGESSCRTDLEMPDTQRVLLQELLKTGKPVVLVLFTGRPLVLNWEQENVPAILNVWFGGSEAALAIGDVLFGNVNPSGKLTTTFPKSVGQIPLFYNHKNTGRPLPQGAWFQKFRSNYLDVDNEPLYPFGYGLSYTTFSYSDITLDKSSMNINGEIMATVTVTNTGKYDGSEVVQLYIRDLIGSVTRPVKELKGFEKIFLKAGESKQVSFKLTADMLKFYNYNLDFVCEPGDFEVMIGGDSRDVNKALFSLQ
AAATCTCCTGTCGATATGGATCGCTTTATTGATGATCTGATGAAGAAGATGACTCTGGAAGAGAAAATCGGCCAGTTGAACTTGCCTGTTACGGGTGAAATAACCACCGGACAAGCCAAGAGTAGTAATGTGGCTAAGCGTATCCGTGCCGGTGAAGTGGGCGGACTCTTTAACTTGAAAGGCGTGGAGCGTATTCGTGACGTTCAGAAACAGGCAGTAGAAGAAAGTCGTCTGGGTATTCCTCTTTTATTTGGTATGGATGTAATTCATGGATACGAAACGGTATTTCCTATTCCTCTGGGATTATCCTGTACCTGGAACATGACAGCTATTGAAGAATCTGCACGTATTGCTGCTATCGAAGCCAGTGCTGATGGTATTTGCTGGACATTCAGTCCGATGGTGGATGTTTCCCGTGATCCCCGTTGGGGACGAGTTTCCGAAGGGAATGGTGAAGATCCCTTCTTGGGAGCGGAGATTGCGCGTGCTATGGTACGTGGTTATCAAGGGAAAGATATGAGTAGTAATGATGAAATTATGGCTTGCGTGAAGCACTTTGCGTTATATGGGGCATCAGAAGCCGGACGCGACTATAATACAGTGGATATGAGTCATCAACGTATGTTCAACGAATATATGTTACCTTATCAGGCTGCCGTGGAAGAAGGTGTGGGTAGTGTGATGGCTTCATTCAATGAAGTGGATGGTGTACCGGCTACCGGAAATAAGTGGCTGATGACCGATGTACTTCGTAAGCAGTGGAATTTTGATGGGTTCGTTGTGACGGACTATACCGGTATCACTGAAATGACCGATCATGGTATGGGTGATACACAAACAGTTGCAGCCCTGGCTCTGAATGCAGGTGTCGATATGGATATGGTGAGCGATGCTTTTACAAGCACACTTAAAAAATCTCTGGAAGAAGGAAAAGTTTCAGTAAAGGCTGTTGATGCTGCTTGTCGCCGTATTCTGGAAGCTAAGTATAAGCTGGGGCTTTTTGATAATCCCTATAAATATTGTGATATAACCCGTCCTAAAAAACAAATCTTTACAAAAGAACACCGCGCTATAGCCCGTAAGACAGCTTCGGAAAGCTTTGTTCTCTTGAAGAATGAGAATAGTGTACTCCCTCTGGCAAAGAAAGGTACCATTGCTGTAGTAGGTCCTTTGGCCGATAGCCGTAGCAATATGCCGGGCACGTGGAGTGTGGCCGCTGTGATGAACAAATATCCTTCTTTGATTGAAGGCTTGAAAGAAGTAGTGGGAGGCAAGGCTAAAATTCTTACGGCTAAAGGAAGTAATCTGATGAGTGATGCCGAATACGAAGAACGTGCTACTATGTTTGGCCGTACTCTGCATCGTGACAATCGTACAGATAAGGAACTGCTGGATGAGGCGCTTGCTGTAGCTGCCAAGTCTGACGTGATTGTTGCTGCTTTGGGTGAGTCTTCCGAGATGAGCGGTGAAAGTAGTTGCCGTACAGACCTCGAAATGCCGGATACGCAACGTGTACTTTTGCAGGAATTGTTGAAAACCGGCAAACCGGTGGTATTGGTGTTGTTTACCGGTCGTCCGTTAGTATTGAATTGGGAGCAGGAAAATGTACCTGCTATTCTGAATGTGTGGTTTGGTGGTAGTGAAGCTGCTCTTGCCATTGGTGATGTACTGTTTGGAAATGTAAATCCGAGTGGCAAACTTACTACTACTTTTCCGAAGAGTGTAGGACAGATTCCTTTGTTCTATAACCATAAGAATACTGGTCGTCCTTTGCCTCAAGGGGCCTGGTTCCAGAAGTTCCGTAGCAATTATCTGGATGTAGATAACGAACCGCTTTATCCGTTTGGATATGGCTTGAGCTATACTACTTTCTCTTATAGTGATATTACATTGGATAAATCGTCCATGAATATCAATGGAGAGATTATGGCAACTGTAACGGTAACCAATACAGGTAAGTATGACGGTTCGGAAGTAGTGCAGCTATATATCCGCGATCTTATAGGCAGTGTAACACGTCCGGTGAAAGAACTGAAAGGCTTTGAAAAAATCTTCTTGAAAGCCGGTGAATCCAAACAAGTGTCTTTCAAGTTAACAGCTGATATGTTGAAGTTCTACAATTACAATCTGGATTTTGTGTGCGAACCGGGTGACTTTGAAGTAATGATAGGTGGTGATAGCCGTGATGTGAATAAGGCCTTATTTTCGCTTCAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,575 total letters Query= APC115086.115 Length=2619 Score E Sequences producing significant alignments: (Bits) Value wt 3979 0.0 12B_B6_APC115086.115_B06.seq 1065 0.0 K S P V D M D R F I D D L M K K M T L E E K I G Q L N L P V Query_1 202 AAATCTCCTGTCGATATGGATCGCTTTATTGATGATCTGATGAAGAAGATGACTCTGGAAGAGAAAATCGGCCAGTTGAACTTGCCTGTT 291 2 1 .......................................................................................... 90 2 867 ............. 879 T G E I T T G Q A K S S N V A K R I R A G E V G G L F N Query_1 292 ACGGGTGAAATAACCACCGGACAAGCCAAGAGTAGT-AATGTGGCT-AAGCGTATCCGTG-CCGGTGAAG-TGGGC--GGACTCTTTAAC 375 2 91 ....................................-.........-.............-.........-.....--............ 174 2 2095 ..... 2091 2 519 .......-.... 529 1 837 ..A......N..G...N..T...N.A....NNAA....N....G.N...CG..CN....A... 775 1 419 .......-.... 409 L K G V E R I R D V Q K Q A V E E S R L G I P L L F Query_1 376 -TTGAAA-GGCGTGGA--GCGTATT-CGTGACGTTCAGAA--ACAGGC-AGTAGAAG-AAAGTCGTCTGGGTA-TTCCTCTTTTATTTGG 455 2 175 -......-........--.......-..............--......-........-...............-................ 254 2 2090 -......-. 2084 1 774 C......A...C...GNG.A...N.N.N..G.N...C.G.TA....C.N....N...N...N......NN...N.........G..AN.. 685 G M D V I H G Y E T V F P I P L G L S C T W N M T A I E E S Query_1 456 -TATGGATGTA-ATTCATGGATACGAAACGGTATTTCCTATTCCTCTGGGATTATCCTGTACCTGGAACATGACAGCTATTGAAGAATCT 543 2 255 -..........-.............................................................................. 342 1 684 AN.N......NN....................N..-....................................T.....N.C....N.... 596 A R I A A I E A S A D G I C W T F S P M V D V S R D P R W G Query_1 544 GCACGTATTGCTGCTATCGAAGCCAGTGCTGATGGTATTTGCTGGACATTCAGTCCGATGGTGGATGTTTCCCGTGATCCCCGTTGGGGA 633 2 343 .......................................................................................... 432 1 595 N.....N.....A.N..N...N....N...........N.....N............N.N.............................. 506 R V S E G N G E D P F L G A E I A R A M V R G Y Q G K D M S Query_1 634 CGAGTTTCCGAAGGGAATGGTGAAGATCCCTTCTTGGGAGCGGAGATTGCGCGTGCTATGGTACGTGGTTATCAAGGGAAAGATATGAGT 723 2 433 .......................................................................................... 522 2 120 .... 123 1 505 ..........N.NN.....A..............N.................N.............T.N..................... 416 S N D E I M A C V K H F A L Y G A S E A G R D Y N T V D M S Query_1 724 AGTAATGATGAAATTATGGCTTGCGTGAAGCACTTTGCGTTATATGGGGCATCAGAAGCCGGACGCGACTATAATACAGTGGATATGAGT 813 2 523 .......................................................................................... 612 2 124 ....... 130 1 415 ..................................N....................................................... 326 H Q R M F N E Y M L P Y Q A A V E E G V G S V M A S A N E V Query_1 814 CATCAACGTATGTTCAACGAATATATGTTACCTTATCAGGCTGCCGTGGAAGAAGGTGTGGGTAGTGTGATGGCTTCAGCTAATGAAGTG 903 2 613 ..............................................................................TTC......... 702 1 325 .......................................................................................... 236 D G V P A T G N K W L M T D V L R K Q W N F D G F V V T D Y Query_1 904 GATGGTGTACCGGCTACCGGAAATAAGTGGCTGATGACCGATGTACTTCGTAAGCAGTGGAATTTTGATGGGTTCGTTGTGACGGACTAT 993 2 703 .......................................................................................... 792 1 235 ................................................................N......................... 146 T G I T E M T D H G M G D T Q T V A A L A L N A G V D M D Query_1 994 ACCGGTATCACTGAAATGACCGATCATGGTATGGGTGATACACAAACAGTTGCAGCCCTGGCTCTGAATGCAGGTGTCGATA-TGGATAT 1082 2 793 ..................................................................................-....... 881 2 9 ........-..... 21 1 145 .....................................................................A....N.......N....... 56 M V S D A F T S T L K K S L E E G K V S V K A V D A A C R Query_1 1083 GGTGAG-CGATGCTTTTA-CAAGCA-CACTTAAAAAATCTCTGGAAGAAGGAAAAGTTTCAGTAAAGGCTGTTGATGCTGCTTGTCGCCG 1169 2 882 ......-...........-......-................................................................ 968 1 55 ......T........N.NG......C........N......-.N.GN...... 4 R I L E A K Y K L G L F D N P Y K Y C D I T R P K K Q I F T Query_1 1170 TATTCTGGAAGCTAAGTATAAGCTGGGGCTTTTTGATAATCCCTATAAATATTGTGATATAACCCGTCCTAAAAAACAAATCTTTACAAA 1259 2 969 .......................................................................................... 1058 K E H R A I A R K T A S E S F V L L K N E N S V L P L A K K Query_1 1260 AGAACACCGCGCTATAGCCCGTAAGACAGCTTCGGAAAGCTTTGTTCTCTTGAAGAATGAGAATAGTGTACTCCCTCTGGCAAAGAAAGG 1349 2 1059 .......................................................................................... 1148 G T I A V V G P L A D S R S N M P G T W S V A A V M N K Y P Query_1 1350 TACCATTGCTGTAGTAGGTCCTTTGGCCGATAGCCGTAGCAATATGCCGGGCACGTGGAGTGTGGCCGCTGTGATGAACAAATATCCTTC 1439 2 1149 .......................................................................................... 1238 S L I E G L K E V V G G K A K I L T A K G S N L M S D A E Y Query_1 1440 TTTGATTGAAGGCTTGAAAGAAGTAGTGGGAGGCAAGGCTAAAATTCTTACGGCTAAAGGAAGTAATCTGATGAGTGATGCCGAATACGA 1529 2 1239 .......................................................................................... 1328 E E R A T M F G R T L H R D N R T D K E L L D E A L A V A A Query_1 1530 AGAACGTGCTACTATGTTTGGCCGTACTCTGCATCGTGACAATCGTACAGATAAGGAACTGCTGGATGAGGCGCTTGCTGTAGCTGCCAA 1619 2 1329 .......................................................................................... 1418 K S D V I V A A L G E S S E M S G E S S C R T D L E M P D T Query_1 1620 GTCTGACGTGATTGTTGCTGCTTTGGGTGAGTCTTCCGAGATGAGCGGTGAAAGTAGTTGCCGTACAGACCTCGAAATGCCGGATACGCA 1709 2 1419 .......................................................................................... 1508 Q R V L L Q E L L K T G K P V V L V L F T G R P L V L N W E Query_1 1710 ACGTGTACTTTTGCAGGAATTGTTGAAAACCGGCAAACCGGTGGTATTGGTGTTGTTTACCGGTCGTCCGTTAGTATTGAATTGGGAGCA 1799 2 1509 .......................................................................................... 1598 Q E N V P A I L N V W F G G S E A A L A I G D V L F G N V N Query_1 1800 GGAAAATGTACCTGCTATTCTGAATGTGTGGTTTGGTGGTAGTGAAGCTGCTCTTGCCATTGGTGATGTACTGTTTGGAAATGTAAATCC 1889 2 1599 .......................................................................................... 1688 P S G K L T T T F P K S V G Q I P L F Y N H K N T G R P L P Query_1 1890 GAGTGGCAAACTTACTACTACTTTTCCGAAGAGTGTAGGACAGATTCCTTTGTTCTATAACCATAAGAATACTGGTCGTCCTTTGCCTCA 1979 2 1689 .......................................................................................... 1778 Q G A W F Q K F R S N Y L D V D N E P L Y P F G Y G L S Y T Query_1 1980 AGGGGCCTGGTTCCAGAAGTTCCGTAGCAATTATCTGGATGTAGATAACGAACCGCTTTATCCGTTTGGATATGGCTTGAGCTATACTAC 2069 2 1779 .......................................................................................... 1868 T F S Y S D I T L D K S S M N I N G E I M A T V T V T N T G Query_1 2070 TTTCTCTTATAGTGATATTACATTGGATAAATCGTCCATGAATATCAATGGAGAGATTATGGCAACTGTAACGGTAACCAATACAGGTAA 2159 2 1869 .......................................................................................... 1958 K Y D G S E V V Q L Y I R D L I G S V T R P V K E L K G F E Query_1 2160 GTATGACGGTTCGGAAGTAGTGCAGCTATATATCCGCGATCTTATAGGCAGTGTAACACGTCCGGTGAAAGAACTGAAAGGCTTTGAAAA 2249 2 1959 .......................................................................................... 2048 K I F L K A G E S K Q V S F K L T A D M L K F Y N Y N L D F Query_1 2250 AATCTTCTTGAAAGCCGGTGAATCCAAACAAGTGTCTTTCAAGTTAACAGCTGATATGTTGAAGTTCTACAATTACAATCTGGATTTTGT 2339 2 2049 .......................................................................................... 2138 2 181 ............ 170 V C E P G D F E V M I G G D S R D V N K A L F S L Q Query_1 2340 GTGCGAACCGGGTGACTTTGAAGTAATGATAGGTGGTGATAGCCGTGATGTGAATAAGGCCTTATTTTCGCTTCAA 2415 2 2139 ............................................................................ 2214 2 2204 .............. 2191 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 9169448 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 3,575 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115086.116 (APC115086.29-766.P(766).Y292A.pMCSG68)
KSPVDMDRFIDDLMKKMTLEEKIGQLNLPVTGEITTGQAKSSNVAKRIRAGEVGGLFNLKGVERIRDVQKQAVEESRLGIPLLFGMDVIHGYETVFPIPLGLSCTWNMTAIEESARIAAIEASADGICWTFSPMVDVSRDPRWGRVSEGNGEDPFLGAEIARAMVRGYQGKDMSSNDEIMACVKHFALYGASEAGRDYNTVDMSHQRMFNEYMLPYQAAVEEGVGSVMASFNEVDGVPATGNKWLMTDVLRKQWNFDGFVVTDYTGITEMTDHGMGDTQTVAALALNAGVDMDMVSDAFTSTLKKSLEEGKVSVKAVDAACRRILEAKYKLGLFDNPYKYCDITRPKKQIFTKEHRAIARKTASESFVLLKNENSVLPLAKKGTIAVVGPLADSRSNMPGTWSVAAVMNKYPSLIEGLKEVVGGKAKILTAKGSNLMSDAEYEERATMFGRTLHRDNRTDKELLDEALAVAAKSDVIVAALGESSEMSGESSCRTDLEMPDTQRVLLQELLKTGKPVVLVLFTGRPLVLNWEQENVPAILNVWFGGSEAALAIGDVLFGNVNPSGKLTTTFPKSVGQIPLFYNHKNTGRPLPQGAWFQKFRSNYLDVDNEPLYPFGYGLSYTTFSYSDITLDKSSMNINGEIMATVTVTNTGKYDGSEVVQLYIRDLIGSVTRPVKELKGFEKIFLKAGESKQVSFKLTADMLKFYNYNLDFVCEPGDFEVMIGGDSRDVNKALFSLQ
AAATCTCCTGTCGATATGGATCGCTTTATTGATGATCTGATGAAGAAGATGACTCTGGAAGAGAAAATCGGCCAGTTGAACTTGCCTGTTACGGGTGAAATAACCACCGGACAAGCCAAGAGTAGTAATGTGGCTAAGCGTATCCGTGCCGGTGAAGTGGGCGGACTCTTTAACTTGAAAGGCGTGGAGCGTATTCGTGACGTTCAGAAACAGGCAGTAGAAGAAAGTCGTCTGGGTATTCCTCTTTTATTTGGTATGGATGTAATTCATGGATACGAAACGGTATTTCCTATTCCTCTGGGATTATCCTGTACCTGGAACATGACAGCTATTGAAGAATCTGCACGTATTGCTGCTATCGAAGCCAGTGCTGATGGTATTTGCTGGACATTCAGTCCGATGGTGGATGTTTCCCGTGATCCCCGTTGGGGACGAGTTTCCGAAGGGAATGGTGAAGATCCCTTCTTGGGAGCGGAGATTGCGCGTGCTATGGTACGTGGTTATCAAGGGAAAGATATGAGTAGTAATGATGAAATTATGGCTTGCGTGAAGCACTTTGCGTTATATGGGGCATCAGAAGCCGGACGCGACTATAATACAGTGGATATGAGTCATCAACGTATGTTCAACGAATATATGTTACCTTATCAGGCTGCCGTGGAAGAAGGTGTGGGTAGTGTGATGGCTTCATTCAATGAAGTGGATGGTGTACCGGCTACCGGAAATAAGTGGCTGATGACCGATGTACTTCGTAAGCAGTGGAATTTTGATGGGTTCGTTGTGACGGACTATACCGGTATCACTGAAATGACCGATCATGGTATGGGTGATACACAAACAGTTGCAGCCCTGGCTCTGAATGCAGGTGTCGATATGGATATGGTGAGCGATGCTTTTACAAGCACACTTAAAAAATCTCTGGAAGAAGGAAAAGTTTCAGTAAAGGCTGTTGATGCTGCTTGTCGCCGTATTCTGGAAGCTAAGTATAAGCTGGGGCTTTTTGATAATCCCTATAAATATTGTGATATAACCCGTCCTAAAAAACAAATCTTTACAAAAGAACACCGCGCTATAGCCCGTAAGACAGCTTCGGAAAGCTTTGTTCTCTTGAAGAATGAGAATAGTGTACTCCCTCTGGCAAAGAAAGGTACCATTGCTGTAGTAGGTCCTTTGGCCGATAGCCGTAGCAATATGCCGGGCACGTGGAGTGTGGCCGCTGTGATGAACAAATATCCTTCTTTGATTGAAGGCTTGAAAGAAGTAGTGGGAGGCAAGGCTAAAATTCTTACGGCTAAAGGAAGTAATCTGATGAGTGATGCCGAATACGAAGAACGTGCTACTATGTTTGGCCGTACTCTGCATCGTGACAATCGTACAGATAAGGAACTGCTGGATGAGGCGCTTGCTGTAGCTGCCAAGTCTGACGTGATTGTTGCTGCTTTGGGTGAGTCTTCCGAGATGAGCGGTGAAAGTAGTTGCCGTACAGACCTCGAAATGCCGGATACGCAACGTGTACTTTTGCAGGAATTGTTGAAAACCGGCAAACCGGTGGTATTGGTGTTGTTTACCGGTCGTCCGTTAGTATTGAATTGGGAGCAGGAAAATGTACCTGCTATTCTGAATGTGTGGTTTGGTGGTAGTGAAGCTGCTCTTGCCATTGGTGATGTACTGTTTGGAAATGTAAATCCGAGTGGCAAACTTACTACTACTTTTCCGAAGAGTGTAGGACAGATTCCTTTGTTCTATAACCATAAGAATACTGGTCGTCCTTTGCCTCAAGGGGCCTGGTTCCAGAAGTTCCGTAGCAATTATCTGGATGTAGATAACGAACCGCTTTATCCGTTTGGATATGGCTTGAGCTATACTACTTTCTCTTATAGTGATATTACATTGGATAAATCGTCCATGAATATCAATGGAGAGATTATGGCAACTGTAACGGTAACCAATACAGGTAAGTATGACGGTTCGGAAGTAGTGCAGCTATATATCCGCGATCTTATAGGCAGTGTAACACGTCCGGTGAAAGAACTGAAAGGCTTTGAAAAAATCTTCTTGAAAGCCGGTGAATCCAAACAAGTGTCTTTCAAGTTAACAGCTGATATGTTGAAGTTCTACAATTACAATCTGGATTTTGTGTGCGAACCGGGTGACTTTGAAGTAATGATAGGTGGTGATAGCCGTGATGTGAATAAGGCCTTATTTTCGCTTCAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,224 total letters Query= APC115086.116 Length=2619 Score E Sequences producing significant alignments: (Bits) Value wt 3984 0.0 K S P V D M D R F I D D L M K K M T L E E K I G Q L N L P V Query_1 202 AAATCTCCTGTCGATATGGATCGCTTTATTGATGATCTGATGAAGAAGATGACTCTGGAAGAGAAAATCGGCCAGTTGAACTTGCCTGTT 291 2 1 .......................................................................................... 90 2 867 ............. 879 T G E I T T G Q A K S S N V A K R I R A G E V G G L F N L K Query_1 292 ACGGGTGAAATAACCACCGGACAAGCCAAGAGTAGTAATGTGGCTAAGCGTATCCGTGCCGGTGAAGTGGGCGGACTCTTTAACTTGAAA 381 2 91 .......................................................................................... 180 2 2095 ........... 2085 2 519 ........... 529 G V E R I R D V Q K Q A V E E S R L G I P L L F G M D V I H Query_1 382 GGCGTGGAGCGTATTCGTGACGTTCAGAAACAGGCAGTAGAAGAAAGTCGTCTGGGTATTCCTCTTTTATTTGGTATGGATGTAATTCAT 471 2 181 .......................................................................................... 270 2 2084 . 2084 G Y E T V F P I P L G L S C T W N M T A I E E S A R I A A I Query_1 472 GGATACGAAACGGTATTTCCTATTCCTCTGGGATTATCCTGTACCTGGAACATGACAGCTATTGAAGAATCTGCACGTATTGCTGCTATC 561 2 271 .......................................................................................... 360 E A S A D G I C W T F S P M V D V S R D P R W G R V S E G N Query_1 562 GAAGCCAGTGCTGATGGTATTTGCTGGACATTCAGTCCGATGGTGGATGTTTCCCGTGATCCCCGTTGGGGACGAGTTTCCGAAGGGAAT 651 2 361 .......................................................................................... 450 G E D P F L G A E I A R A M V R G Y Q G K D M S S N D E I M Query_1 652 GGTGAAGATCCCTTCTTGGGAGCGGAGATTGCGCGTGCTATGGTACGTGGTTATCAAGGGAAAGATATGAGTAGTAATGATGAAATTATG 741 2 451 .......................................................................................... 540 2 120 ........... 130 A C V K H F A L Y G A S E A G R D Y N T V D M S H Q R M F N Query_1 742 GCTTGCGTGAAGCACTTTGCGTTATATGGGGCATCAGAAGCCGGACGCGACTATAATACAGTGGATATGAGTCATCAACGTATGTTCAAC 831 2 541 .......................................................................................... 630 E Y M L P Y Q A A V E E G V G S V M A S F N E V D G V P A T Query_1 832 GAATATATGTTACCTTATCAGGCTGCCGTGGAAGAAGGTGTGGGTAGTGTGATGGCTTCATTCAATGAAGTGGATGGTGTACCGGCTACC 921 2 631 .......................................................................................... 720 G N K W L M T D V L R K Q W N F D G F V V T D A T G I T E M Query_1 922 GGAAATAAGTGGCTGATGACCGATGTACTTCGTAAGCAGTGGAATTTTGATGGGTTCGTTGTGACGGACGCTACCGGTATCACTGAAATG 1011 2 721 .....................................................................TA................... 810 T D H G M G D T Q T V A A L A L N A G V D M D M V S D A F T Query_1 1012 ACCGATCATGGTATGGGTGATACACAAACAGTTGCAGCCCTGGCTCTGAATGCAGGTGTCGATATGGATATGGTGAGCGATGCTTTTACA 1101 2 811 .......................................................................................... 900 2 9 ............. 21 S T L K K S L E E G K V S V K A V D A A C R R I L E A K Y K Query_1 1102 AGCACACTTAAAAAATCTCTGGAAGAAGGAAAAGTTTCAGTAAAGGCTGTTGATGCTGCTTGTCGCCGTATTCTGGAAGCTAAGTATAAG 1191 2 901 .......................................................................................... 990 L G L F D N P Y K Y C D I T R P K K Q I F T K E H R A I A R Query_1 1192 CTGGGGCTTTTTGATAATCCCTATAAATATTGTGATATAACCCGTCCTAAAAAACAAATCTTTACAAAAGAACACCGCGCTATAGCCCGT 1281 2 991 .......................................................................................... 1080 K T A S E S F V L L K N E N S V L P L A K K G T I A V V G P Query_1 1282 AAGACAGCTTCGGAAAGCTTTGTTCTCTTGAAGAATGAGAATAGTGTACTCCCTCTGGCAAAGAAAGGTACCATTGCTGTAGTAGGTCCT 1371 2 1081 .......................................................................................... 1170 L A D S R S N M P G T W S V A A V M N K Y P S L I E G L K E Query_1 1372 TTGGCCGATAGCCGTAGCAATATGCCGGGCACGTGGAGTGTGGCCGCTGTGATGAACAAATATCCTTCTTTGATTGAAGGCTTGAAAGAA 1461 2 1171 .......................................................................................... 1260 V V G G K A K I L T A K G S N L M S D A E Y E E R A T M F G Query_1 1462 GTAGTGGGAGGCAAGGCTAAAATTCTTACGGCTAAAGGAAGTAATCTGATGAGTGATGCCGAATACGAAGAACGTGCTACTATGTTTGGC 1551 2 1261 .......................................................................................... 1350 R T L H R D N R T D K E L L D E A L A V A A K S D V I V A A Query_1 1552 CGTACTCTGCATCGTGACAATCGTACAGATAAGGAACTGCTGGATGAGGCGCTTGCTGTAGCTGCCAAGTCTGACGTGATTGTTGCTGCT 1641 2 1351 .......................................................................................... 1440 L G E S S E M S G E S S C R T D L E M P D T Q R V L L Q E L Query_1 1642 TTGGGTGAGTCTTCCGAGATGAGCGGTGAAAGTAGTTGCCGTACAGACCTCGAAATGCCGGATACGCAACGTGTACTTTTGCAGGAATTG 1731 2 1441 .......................................................................................... 1530 L K T G K P V V L V L F T G R P L V L N W E Q E N V P A I L Query_1 1732 TTGAAAACCGGCAAACCGGTGGTATTGGTGTTGTTTACCGGTCGTCCGTTAGTATTGAATTGGGAGCAGGAAAATGTACCTGCTATTCTG 1821 2 1531 .......................................................................................... 1620 N V W F G G S E A A L A I G D V L F G N V N P S G K L T T T Query_1 1822 AATGTGTGGTTTGGTGGTAGTGAAGCTGCTCTTGCCATTGGTGATGTACTGTTTGGAAATGTAAATCCGAGTGGCAAACTTACTACTACT 1911 2 1621 .......................................................................................... 1710 F P K S V G Q I P L F Y N H K N T G R P L P Q G A W F Q K F Query_1 1912 TTTCCGAAGAGTGTAGGACAGATTCCTTTGTTCTATAACCATAAGAATACTGGTCGTCCTTTGCCTCAAGGGGCCTGGTTCCAGAAGTTC 2001 2 1711 .......................................................................................... 1800 R S N Y L D V D N E P L Y P F G Y G L S Y T T F S Y S D I T Query_1 2002 CGTAGCAATTATCTGGATGTAGATAACGAACCGCTTTATCCGTTTGGATATGGCTTGAGCTATACTACTTTCTCTTATAGTGATATTACA 2091 2 1801 .......................................................................................... 1890 L D K S S M N I N G E I M A T V T V T N T G K Y D G S E V V Query_1 2092 TTGGATAAATCGTCCATGAATATCAATGGAGAGATTATGGCAACTGTAACGGTAACCAATACAGGTAAGTATGACGGTTCGGAAGTAGTG 2181 2 1891 .......................................................................................... 1980 Q L Y I R D L I G S V T R P V K E L K G F E K I F L K A G E Query_1 2182 CAGCTATATATCCGCGATCTTATAGGCAGTGTAACACGTCCGGTGAAAGAACTGAAAGGCTTTGAAAAAATCTTCTTGAAAGCCGGTGAA 2271 2 1981 .......................................................................................... 2070 S K Q V S F K L T A D M L K F Y N Y N L D F V C E P G D F E Query_1 2272 TCCAAACAAGTGTCTTTCAAGTTAACAGCTGATATGTTGAAGTTCTACAATTACAATCTGGATTTTGTGTGCGAACCGGGTGACTTTGAA 2361 2 2071 .......................................................................................... 2160 2 181 ............ 170 V M I G G D S R D V N K A L F S L Q Query_1 2362 GTAATGATAGGTGGTGATAGCCGTGATGTGAATAAGGCCTTATTTTCGCTTCAA 2415 2 2161 ...................................................... 2214 2 2204 .............. 2191 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5664128 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 2,224 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115086.118 (APC115086.29-766.P(766).W430F.pMCSG68)
KSPVDMDRFIDDLMKKMTLEEKIGQLNLPVTGEITTGQAKSSNVAKRIRAGEVGGLFNLKGVERIRDVQKQAVEESRLGIPLLFGMDVIHGYETVFPIPLGLSCTWNMTAIEESARIAAIEASADGICWTFSPMVDVSRDPRWGRVSEGNGEDPFLGAEIARAMVRGYQGKDMSSNDEIMACVKHFALYGASEAGRDYNTVDMSHQRMFNEYMLPYQAAVEEGVGSVMASFNEVDGVPATGNKWLMTDVLRKQWNFDGFVVTDYTGITEMTDHGMGDTQTVAALALNAGVDMDMVSDAFTSTLKKSLEEGKVSVKAVDAACRRILEAKYKLGLFDNPYKYCDITRPKKQIFTKEHRAIARKTASESFVLLKNENSVLPLAKKGTIAVVGPLADSRSNMPGTWSVAAVMNKYPSLIEGLKEVVGGKAKILTAKGSNLMSDAEYEERATMFGRTLHRDNRTDKELLDEALAVAAKSDVIVAALGESSEMSGESSCRTDLEMPDTQRVLLQELLKTGKPVVLVLFTGRPLVLNWEQENVPAILNVWFGGSEAALAIGDVLFGNVNPSGKLTTTFPKSVGQIPLFYNHKNTGRPLPQGAWFQKFRSNYLDVDNEPLYPFGYGLSYTTFSYSDITLDKSSMNINGEIMATVTVTNTGKYDGSEVVQLYIRDLIGSVTRPVKELKGFEKIFLKAGESKQVSFKLTADMLKFYNYNLDFVCEPGDFEVMIGGDSRDVNKALFSLQ
AAATCTCCTGTCGATATGGATCGCTTTATTGATGATCTGATGAAGAAGATGACTCTGGAAGAGAAAATCGGCCAGTTGAACTTGCCTGTTACGGGTGAAATAACCACCGGACAAGCCAAGAGTAGTAATGTGGCTAAGCGTATCCGTGCCGGTGAAGTGGGCGGACTCTTTAACTTGAAAGGCGTGGAGCGTATTCGTGACGTTCAGAAACAGGCAGTAGAAGAAAGTCGTCTGGGTATTCCTCTTTTATTTGGTATGGATGTAATTCATGGATACGAAACGGTATTTCCTATTCCTCTGGGATTATCCTGTACCTGGAACATGACAGCTATTGAAGAATCTGCACGTATTGCTGCTATCGAAGCCAGTGCTGATGGTATTTGCTGGACATTCAGTCCGATGGTGGATGTTTCCCGTGATCCCCGTTGGGGACGAGTTTCCGAAGGGAATGGTGAAGATCCCTTCTTGGGAGCGGAGATTGCGCGTGCTATGGTACGTGGTTATCAAGGGAAAGATATGAGTAGTAATGATGAAATTATGGCTTGCGTGAAGCACTTTGCGTTATATGGGGCATCAGAAGCCGGACGCGACTATAATACAGTGGATATGAGTCATCAACGTATGTTCAACGAATATATGTTACCTTATCAGGCTGCCGTGGAAGAAGGTGTGGGTAGTGTGATGGCTTCATTCAATGAAGTGGATGGTGTACCGGCTACCGGAAATAAGTGGCTGATGACCGATGTACTTCGTAAGCAGTGGAATTTTGATGGGTTCGTTGTGACGGACTATACCGGTATCACTGAAATGACCGATCATGGTATGGGTGATACACAAACAGTTGCAGCCCTGGCTCTGAATGCAGGTGTCGATATGGATATGGTGAGCGATGCTTTTACAAGCACACTTAAAAAATCTCTGGAAGAAGGAAAAGTTTCAGTAAAGGCTGTTGATGCTGCTTGTCGCCGTATTCTGGAAGCTAAGTATAAGCTGGGGCTTTTTGATAATCCCTATAAATATTGTGATATAACCCGTCCTAAAAAACAAATCTTTACAAAAGAACACCGCGCTATAGCCCGTAAGACAGCTTCGGAAAGCTTTGTTCTCTTGAAGAATGAGAATAGTGTACTCCCTCTGGCAAAGAAAGGTACCATTGCTGTAGTAGGTCCTTTGGCCGATAGCCGTAGCAATATGCCGGGCACGTGGAGTGTGGCCGCTGTGATGAACAAATATCCTTCTTTGATTGAAGGCTTGAAAGAAGTAGTGGGAGGCAAGGCTAAAATTCTTACGGCTAAAGGAAGTAATCTGATGAGTGATGCCGAATACGAAGAACGTGCTACTATGTTTGGCCGTACTCTGCATCGTGACAATCGTACAGATAAGGAACTGCTGGATGAGGCGCTTGCTGTAGCTGCCAAGTCTGACGTGATTGTTGCTGCTTTGGGTGAGTCTTCCGAGATGAGCGGTGAAAGTAGTTGCCGTACAGACCTCGAAATGCCGGATACGCAACGTGTACTTTTGCAGGAATTGTTGAAAACCGGCAAACCGGTGGTATTGGTGTTGTTTACCGGTCGTCCGTTAGTATTGAATTGGGAGCAGGAAAATGTACCTGCTATTCTGAATGTGTGGTTTGGTGGTAGTGAAGCTGCTCTTGCCATTGGTGATGTACTGTTTGGAAATGTAAATCCGAGTGGCAAACTTACTACTACTTTTCCGAAGAGTGTAGGACAGATTCCTTTGTTCTATAACCATAAGAATACTGGTCGTCCTTTGCCTCAAGGGGCCTGGTTCCAGAAGTTCCGTAGCAATTATCTGGATGTAGATAACGAACCGCTTTATCCGTTTGGATATGGCTTGAGCTATACTACTTTCTCTTATAGTGATATTACATTGGATAAATCGTCCATGAATATCAATGGAGAGATTATGGCAACTGTAACGGTAACCAATACAGGTAAGTATGACGGTTCGGAAGTAGTGCAGCTATATATCCGCGATCTTATAGGCAGTGTAACACGTCCGGTGAAAGAACTGAAAGGCTTTGAAAAAATCTTCTTGAAAGCCGGTGAATCCAAACAAGTGTCTTTCAAGTTAACAGCTGATATGTTGAAGTTCTACAATTACAATCTGGATTTTGTGTGCGAACCGGGTGACTTTGAAGTAATGATAGGTGGTGATAGCCGTGATGTGAATAAGGCCTTATTTTCGCTTCAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,590 total letters Query= APC115086.118 Length=2619 Score E Sequences producing significant alignments: (Bits) Value wt 3984 0.0 12D_D6_APC115086.118_D06.seq 847 0.0 K S P V D M D R F I D D L M K K M T L E E K I G Q L N L P V Query_1 202 AAATCTCCTGTCGATATGGATCGCTTTATTGATGATCTGATGAAGAAGATGACTCTGGAAGAGAAAATCGGCCAGTTGAACTTGCCTGTT 291 2 1 .......................................................................................... 90 2 867 ............. 879 T G E I T T G Q A K S S N V A K R I R A G E V G G L F N L K Query_1 292 ACGGGTGAAATAACCACCGGACAAGCCAAGAGTAGTAATGTGGCTAAGCGTATCCGTGCCGGTGAAGTGGGCGGACTCTTTAACTTGAAA 381 2 91 .......................................................................................... 180 2 2095 ........... 2085 2 519 ........... 529 G V E R I R D V Q K Q A V E E S R L G I P L L F G M D V I H Query_1 382 GGCGTGGAGCGTATTCGTGACGTTCAGAAACAGGCAGTAGAAGAAAGTCGTCTGGGTATTCCTCTTTTATTTGGTATGGATGTAATTCAT 471 2 181 .......................................................................................... 270 2 2084 . 2084 G Y E T V F P I P L G L S C T W N M T A I E E S A R I A A I Query_1 472 GGATACGAAACGGTATTTCCTATTCCTCTGGGATTATCCTGTACCTGGAACATGACAGCTATTGAAGAATCTGCACGTATTGCTGCTATC 561 2 271 .......................................................................................... 360 E A S A D G I C W T F S P M V D V S R D P R W G R V S E G N Query_1 562 GAAGCCAGTGCTGATGGTATTTGCTGGACATTCAGTCCGATGGTGGATGTTTCCCGTGATCCCCGTTGGGGACGAGTTTCCGAAGGGAAT 651 2 361 .......................................................................................... 450 G E D P F L G A E I A R A M V R G Y Q G K D M S S N D E I M Query_1 652 GGTGAAGATCCCTTCTTGGGAGCGGAGATTGCGCGTGCTATGGTACGTGGTTATCAAGGGAAAGATATGAGTAGTAATGATGAAATTATG 741 2 451 .......................................................................................... 540 2 120 ........... 130 A C V K H F A L Y G A S E A G R D Y N T V D M S H Q R M F N Query_1 742 GCTTGCGTGAAGCACTTTGCGTTATATGGGGCATCAGAAGCCGGACGCGACTATAATACAGTGGATATGAGTCATCAACGTATGTTCAAC 831 2 541 .......................................................................................... 630 E Y M L P Y Q A A V E E G V G S V M A S F N E V D G V P A T Query_1 832 GAATATATGTTACCTTATCAGGCTGCCGTGGAAGAAGGTGTGGGTAGTGTGATGGCTTCATTCAATGAAGTGGATGGTGTACCGGCTACC 921 2 631 .......................................................................................... 720 G N K W L M T D V L R K Q W N F D G F V V T D Y T G I T E M Query_1 922 GGAAATAAGTGGCTGATGACCGATGTACTTCGTAAGCAGTGGAATTTTGATGGGTTCGTTGTGACGGACTATACCGGTATCACTGAAATG 1011 2 721 .......................................................................................... 810 T D H G M G D T Q T V A A L A L N A G V D M D M V S D A F Query_1 1012 ACCGATCATGGTATGGGTGATACACAAACAGTTGCAGCCCTGGCTCTGAATGCAGGTGTCGATATGGATATGGTGAGCG-ATGCTTTTAC 1100 2 811 ...............................................................................-.......... 899 2 9 ............. 21 1 693 ...N..-.....G...N....N. 672 T S T L K K S L E E G K V S V K A V D A A C R R I L E A K Query_1 1101 AAGCACACTTAAA-AAATCTCTGGAAGAAGGAAAAGTTTCAGTAAAGGCTGTTGATGCTGCTTGTCGCCGTATTCTGGAAG-CTAAGT-A 1187 2 900 .............-...................................................................-......-. 986 1 671 ....N.TNN.NTNG....N...N.N.....N.N............N...NA.N..N.G.A.N....C.N............N......T. 582 Y K L G L F D N P Y K Y C D I T R P K K Q I F T K E H R A Query_1 1188 TAAGCTGGGGCTTTTTG-ATAATCCCTATAAATATTGTGATATAACCCGTCC-TAAAAAACAAATCTTTACAAAAGAACACCGCGCT-AT 1274 2 987 .................-..................................-..................................-.. 1073 1 581 .......N.....N...N...N...N.N..N........N....N......NN....N.N..N....GN.....N.NN......N.NG.. 492 I A R K T A S E S F V L L K N E N S V L P L A K K G T I A V Query_1 1275 A-GCCCGTAAGACAGCTTCGGAAAGCTTTGTTCTCTTGAAGAA-TGAGAATAGTGTACTCCCTCTGGCAAAGAAAGGTACCATTGCTGTA 1362 2 1074 .-.........................................-.............................................. 1161 1 491 .N.N..............G.........N......N.......A..................................N....NN..N.. 402 V G P L A D S R S N M P G T F S V A A V M N K Y P S L I E Query_1 1363 GTAGGTCCTTTGGCCGATAG-CCGTAGCAATATGCCGGGCACGTTTAGTGTGGCCGCTGTGATGAACAAATATCCTTCTTTGATTGAAGG 1451 2 1162 ....................-.......................GG............................................ 1250 1 401 ...........NN.......N...........................N..............................N.........N 312 G L K E V V G G K A K I L T A K G S N L M S D A E Y E E R A Query_1 1452 CTTGAAAGAAGTAGTGGGAGGCAAGGCTAAAATTCTTACGGCTAAAGGAAGTAATCTGATGAGTGATGCCGAATACGAAGAACGTGCTAC 1541 2 1251 .......................................................................................... 1340 1 311 ..N..NN.............N..................................................N.................. 222 T M F G R T L H R D N R T D K E L L D E A L A V A A K S D V Query_1 1542 TATGTTTGGCCGTACTCTGCATCGTGACAATCGTACAGATAAGGAACTGCTGGATGAGGCGCTTGCTGTAGCTGCCAAGTCTGACGTGAT 1631 2 1341 .......................................................................................... 1430 1 221 .........................................N..................N............................. 132 I V A A L G E S S E M S G E S S C R T D L E M P D T Q R V L Query_1 1632 TGTTGCTGCTTTGGGTGAGTCTTCCGAGATGA-GCGGTGAAAGTAGTTGCCGTACAGACCTCGAAATGCCGGATACGCAA-CGTGTACTT 1719 2 1431 ................................-...............................................-......... 1518 1 131 ................................N......................N......A.................G..N...... 42 L Q E L L K T G K P V V L V L F T G R P L V L N W E Q E N V Query_1 1720 TTGCAGGAATTGTTGAAAACCGGCAAACCGGTGGTATTGGTGTTGTTTACCGGTCGTCCGTTAGTATTGAATTGGGAGCAGGAAAATGTA 1809 2 1519 .......................................................................................... 1608 1 41 ..N....N..N.-.........N... 17 P A I L N V W F G G S E A A L A I G D V L F G N V N P S G K Query_1 1810 CCTGCTATTCTGAATGTGTGGTTTGGTGGTAGTGAAGCTGCTCTTGCCATTGGTGATGTACTGTTTGGAAATGTAAATCCGAGTGGCAAA 1899 2 1609 .......................................................................................... 1698 L T T T F P K S V G Q I P L F Y N H K N T G R P L P Q G A W Query_1 1900 CTTACTACTACTTTTCCGAAGAGTGTAGGACAGATTCCTTTGTTCTATAACCATAAGAATACTGGTCGTCCTTTGCCTCAAGGGGCCTGG 1989 2 1699 .......................................................................................... 1788 F Q K F R S N Y L D V D N E P L Y P F G Y G L S Y T T F S Y Query_1 1990 TTCCAGAAGTTCCGTAGCAATTATCTGGATGTAGATAACGAACCGCTTTATCCGTTTGGATATGGCTTGAGCTATACTACTTTCTCTTAT 2079 2 1789 .......................................................................................... 1878 S D I T L D K S S M N I N G E I M A T V T V T N T G K Y D G Query_1 2080 AGTGATATTACATTGGATAAATCGTCCATGAATATCAATGGAGAGATTATGGCAACTGTAACGGTAACCAATACAGGTAAGTATGACGGT 2169 2 1879 .......................................................................................... 1968 S E V V Q L Y I R D L I G S V T R P V K E L K G F E K I F L Query_1 2170 TCGGAAGTAGTGCAGCTATATATCCGCGATCTTATAGGCAGTGTAACACGTCCGGTGAAAGAACTGAAAGGCTTTGAAAAAATCTTCTTG 2259 2 1969 .......................................................................................... 2058 K A G E S K Q V S F K L T A D M L K F Y N Y N L D F V C E P Query_1 2260 AAAGCCGGTGAATCCAAACAAGTGTCTTTCAAGTTAACAGCTGATATGTTGAAGTTCTACAATTACAATCTGGATTTTGTGTGCGAACCG 2349 2 2059 .......................................................................................... 2148 2 181 ............ 170 G D F E V M I G G D S R D V N K A L F S L Q Query_1 2350 GGTGACTTTGAAGTAATGATAGGTGGTGATAGCCGTGATGTGAATAAGGCCTTATTTTCGCTTCAA 2415 2 2149 .................................................................. 2214 2 2204 .............. 2191 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 9208478 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 3,590 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115086.119 (APC115086.29-766.P(766).Y292F.pMCSG68)
KSPVDMDRFIDDLMKKMTLEEKIGQLNLPVTGEITTGQAKSSNVAKRIRAGEVGGLFNLKGVERIRDVQKQAVEESRLGIPLLFGMDVIHGYETVFPIPLGLSCTWNMTAIEESARIAAIEASADGICWTFSPMVDVSRDPRWGRVSEGNGEDPFLGAEIARAMVRGYQGKDMSSNDEIMACVKHFALYGASEAGRDYNTVDMSHQRMFNEYMLPYQAAVEEGVGSVMASFNEVDGVPATGNKWLMTDVLRKQWNFDGFVVTDYTGITEMTDHGMGDTQTVAALALNAGVDMDMVSDAFTSTLKKSLEEGKVSVKAVDAACRRILEAKYKLGLFDNPYKYCDITRPKKQIFTKEHRAIARKTASESFVLLKNENSVLPLAKKGTIAVVGPLADSRSNMPGTWSVAAVMNKYPSLIEGLKEVVGGKAKILTAKGSNLMSDAEYEERATMFGRTLHRDNRTDKELLDEALAVAAKSDVIVAALGESSEMSGESSCRTDLEMPDTQRVLLQELLKTGKPVVLVLFTGRPLVLNWEQENVPAILNVWFGGSEAALAIGDVLFGNVNPSGKLTTTFPKSVGQIPLFYNHKNTGRPLPQGAWFQKFRSNYLDVDNEPLYPFGYGLSYTTFSYSDITLDKSSMNINGEIMATVTVTNTGKYDGSEVVQLYIRDLIGSVTRPVKELKGFEKIFLKAGESKQVSFKLTADMLKFYNYNLDFVCEPGDFEVMIGGDSRDVNKALFSLQ
AAATCTCCTGTCGATATGGATCGCTTTATTGATGATCTGATGAAGAAGATGACTCTGGAAGAGAAAATCGGCCAGTTGAACTTGCCTGTTACGGGTGAAATAACCACCGGACAAGCCAAGAGTAGTAATGTGGCTAAGCGTATCCGTGCCGGTGAAGTGGGCGGACTCTTTAACTTGAAAGGCGTGGAGCGTATTCGTGACGTTCAGAAACAGGCAGTAGAAGAAAGTCGTCTGGGTATTCCTCTTTTATTTGGTATGGATGTAATTCATGGATACGAAACGGTATTTCCTATTCCTCTGGGATTATCCTGTACCTGGAACATGACAGCTATTGAAGAATCTGCACGTATTGCTGCTATCGAAGCCAGTGCTGATGGTATTTGCTGGACATTCAGTCCGATGGTGGATGTTTCCCGTGATCCCCGTTGGGGACGAGTTTCCGAAGGGAATGGTGAAGATCCCTTCTTGGGAGCGGAGATTGCGCGTGCTATGGTACGTGGTTATCAAGGGAAAGATATGAGTAGTAATGATGAAATTATGGCTTGCGTGAAGCACTTTGCGTTATATGGGGCATCAGAAGCCGGACGCGACTATAATACAGTGGATATGAGTCATCAACGTATGTTCAACGAATATATGTTACCTTATCAGGCTGCCGTGGAAGAAGGTGTGGGTAGTGTGATGGCTTCATTCAATGAAGTGGATGGTGTACCGGCTACCGGAAATAAGTGGCTGATGACCGATGTACTTCGTAAGCAGTGGAATTTTGATGGGTTCGTTGTGACGGACTATACCGGTATCACTGAAATGACCGATCATGGTATGGGTGATACACAAACAGTTGCAGCCCTGGCTCTGAATGCAGGTGTCGATATGGATATGGTGAGCGATGCTTTTACAAGCACACTTAAAAAATCTCTGGAAGAAGGAAAAGTTTCAGTAAAGGCTGTTGATGCTGCTTGTCGCCGTATTCTGGAAGCTAAGTATAAGCTGGGGCTTTTTGATAATCCCTATAAATATTGTGATATAACCCGTCCTAAAAAACAAATCTTTACAAAAGAACACCGCGCTATAGCCCGTAAGACAGCTTCGGAAAGCTTTGTTCTCTTGAAGAATGAGAATAGTGTACTCCCTCTGGCAAAGAAAGGTACCATTGCTGTAGTAGGTCCTTTGGCCGATAGCCGTAGCAATATGCCGGGCACGTGGAGTGTGGCCGCTGTGATGAACAAATATCCTTCTTTGATTGAAGGCTTGAAAGAAGTAGTGGGAGGCAAGGCTAAAATTCTTACGGCTAAAGGAAGTAATCTGATGAGTGATGCCGAATACGAAGAACGTGCTACTATGTTTGGCCGTACTCTGCATCGTGACAATCGTACAGATAAGGAACTGCTGGATGAGGCGCTTGCTGTAGCTGCCAAGTCTGACGTGATTGTTGCTGCTTTGGGTGAGTCTTCCGAGATGAGCGGTGAAAGTAGTTGCCGTACAGACCTCGAAATGCCGGATACGCAACGTGTACTTTTGCAGGAATTGTTGAAAACCGGCAAACCGGTGGTATTGGTGTTGTTTACCGGTCGTCCGTTAGTATTGAATTGGGAGCAGGAAAATGTACCTGCTATTCTGAATGTGTGGTTTGGTGGTAGTGAAGCTGCTCTTGCCATTGGTGATGTACTGTTTGGAAATGTAAATCCGAGTGGCAAACTTACTACTACTTTTCCGAAGAGTGTAGGACAGATTCCTTTGTTCTATAACCATAAGAATACTGGTCGTCCTTTGCCTCAAGGGGCCTGGTTCCAGAAGTTCCGTAGCAATTATCTGGATGTAGATAACGAACCGCTTTATCCGTTTGGATATGGCTTGAGCTATACTACTTTCTCTTATAGTGATATTACATTGGATAAATCGTCCATGAATATCAATGGAGAGATTATGGCAACTGTAACGGTAACCAATACAGGTAAGTATGACGGTTCGGAAGTAGTGCAGCTATATATCCGCGATCTTATAGGCAGTGTAACACGTCCGGTGAAAGAACTGAAAGGCTTTGAAAAAATCTTCTTGAAAGCCGGTGAATCCAAACAAGTGTCTTTCAAGTTAACAGCTGATATGTTGAAGTTCTACAATTACAATCTGGATTTTGTGTGCGAACCGGGTGACTTTGAAGTAATGATAGGTGGTGATAGCCGTGATGTGAATAAGGCCTTATTTTCGCTTCAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,568 total letters Query= APC115086.119 Length=2619 Score E Sequences producing significant alignments: (Bits) Value wt 3988 0.0 12E_E6_APC115086.119_E06.seq 1260 0.0 K S P V D M D R F I D D L M K K M T L E E K I G Q L N L P V Query_1 202 AAATCTCCTGTCGATATGGATCGCTTTATTGATGATCTGATGAAGAAGATGACTCTGGAAGAGAAAATCGGCCAGTTGAACTTGCCTGTT 291 2 1 .......................................................................................... 90 2 867 ............. 879 1 61 ............. 49 T G E I T T G Q A K S S N V A K R I R A G E V G G Query_1 292 ACGGGTGAA-ATAACC-ACCGG-ACAAGCCAAG--AGTAG-TAATGT-GGCTAA-GCGTATCCGTG----CCGGTG--AAGTGGGCGG-A 366 2 91 .........-......-.....-..........--.....-......-......-...........----......--..........-. 165 2 519 .--.....-..... 529 1 860 .......N......C.....GN...T.....GAN..N.G..N...T..N...A..N.....CC.TTNC......GA..........GN 773 1 410 .--.....-..... 400 L F N L K G V E R I R D V Q K Q A V E E S R L G I P L Query_1 367 CTCTTTAA-CTTGAAA--GGCGTGG-AGC-GTATTCGTGACGTTCAGAAACAGGCAGTAGAAGAAA-GTCGTC-TGGGTATTCCTCTTTT 449 2 166 ........-.......--.......-...-....................................-......-................ 248 2 2095 ....-.......--. 2084 1 772 ........N....N..AN.N.N...G..NA.......................N....N.......A......C................ 683 L F G M D V I H G Y E T V F P I P L G L S C T W N M T A I E Query_1 450 -ATTTGGTATGGATGTAATTCATGGATACGAAA-CGGTATTTCCTATTCCTCTGGGATTATCCTGTACCTGGAACATGACAGCTATTGAA 537 2 249 -................................-........................................................ 336 1 682 T..N...N.....N...................A....................A..............................N.... 593 E S A R I A A I E A S A D G I C W T F S P M V D V S R D P R Query_1 538 GAATCTGCACGTATTGCTGCTATCGAAGCCAGTGCTGATGGTATTTGCTGGACATTCAGTCCGATGGTGGATGTTTCCCGTGATCCCCGT 627 2 337 .......................................................................................... 426 1 592 ....N..................................................................................... 503 W G R V S E G N G E D P F L G A E I A R A M V R G Y Q G K D Query_1 628 TGGGGACGAGTTTCCGAAGGGAATGGTGAAGATCCCTTCTTGGGAGCGGAGATTGCGCGTGCTATGGTACGTGGTTATCAAGGGAAAGAT 717 2 427 .......................................................................................... 516 1 502 ............................................................N............................. 413 M S S N D E I M A C V K H F A L Y G A S E A G R D Y N T V D Query_1 718 ATGAGTAGTAATGATGAAATTATGGCTTGCGTGAAGCACTTTGCGTTATATGGGGCATCAGAAGCCGGACGCGACTATAATACAGTGGAT 807 2 517 .......................................................................................... 606 2 120 ........... 130 1 412 .......................................................................................... 323 M S H Q R M F N E Y M L P Y Q A A V E E G V G S V M A S F N Query_1 808 ATGAGTCATCAACGTATGTTCAACGAATATATGTTACCTTATCAGGCTGCCGTGGAAGAAGGTGTGGGTAGTGTGATGGCTTCATTCAAT 897 2 607 .......................................................................................... 696 1 322 .......................................................................................... 233 E V D G V P A T G N K W L M T D V L R K Q W N F D G F V V T Query_1 898 GAAGTGGATGGTGTACCGGCTACCGGAAATAAGTGGCTGATGACCGATGTACTTCGTAAGCAGTGGAATTTTGATGGGTTCGTTGTGACG 987 2 697 .......................................................................................... 786 1 232 .......................................................................................... 143 D F T G I T E M T D H G M G D T Q T V A A L A L N A G V D Query_1 988 GACTTTACCGGTATCACTGAAATGACCGATCATGGTATGGGTGATACACAAACAGTTGCAGCCCTGGCTCTGAAT-GCAGGTGTCGATAT 1076 2 787 ....A......................................................................-.............. 875 2 9 ......... 17 1 142 ..........................................................................NAA............. 53 M D M V S D A F T S T L K K S L E E G K V S V K A V D A A C Query_1 1077 GGATATGGTGAGCGATGCTTTTACAAGCACACTTAAAAAATCTCTGGAAGAAGGAAAAGTTTCAGTAAAGGCTGTTGATGCTGCTTGTCG 1166 2 876 .......................................................................................... 965 2 18 .... 21 1 52 ................................-...........NA...A.. 2 R R I L E A K Y K L G L F D N P Y K Y C D I T R P K K Q I F Query_1 1167 CCGTATTCTGGAAGCTAAGTATAAGCTGGGGCTTTTTGATAATCCCTATAAATATTGTGATATAACCCGTCCTAAAAAACAAATCTTTAC 1256 2 966 .......................................................................................... 1055 T K E H R A I A R K T A S E S F V L L K N E N S V L P L A K Query_1 1257 AAAAGAACACCGCGCTATAGCCCGTAAGACAGCTTCGGAAAGCTTTGTTCTCTTGAAGAATGAGAATAGTGTACTCCCTCTGGCAAAGAA 1346 2 1056 .......................................................................................... 1145 K G T I A V V G P L A D S R S N M P G T W S V A A V M N K Y Query_1 1347 AGGTACCATTGCTGTAGTAGGTCCTTTGGCCGATAGCCGTAGCAATATGCCGGGCACGTGGAGTGTGGCCGCTGTGATGAACAAATATCC 1436 2 1146 .......................................................................................... 1235 P S L I E G L K E V V G G K A K I L T A K G S N L M S D A E Query_1 1437 TTCTTTGATTGAAGGCTTGAAAGAAGTAGTGGGAGGCAAGGCTAAAATTCTTACGGCTAAAGGAAGTAATCTGATGAGTGATGCCGAATA 1526 2 1236 .......................................................................................... 1325 Y E E R A T M F G R T L H R D N R T D K E L L D E A L A V A Query_1 1527 CGAAGAACGTGCTACTATGTTTGGCCGTACTCTGCATCGTGACAATCGTACAGATAAGGAACTGCTGGATGAGGCGCTTGCTGTAGCTGC 1616 2 1326 .......................................................................................... 1415 A K S D V I V A A L G E S S E M S G E S S C R T D L E M P D Query_1 1617 CAAGTCTGACGTGATTGTTGCTGCTTTGGGTGAGTCTTCCGAGATGAGCGGTGAAAGTAGTTGCCGTACAGACCTCGAAATGCCGGATAC 1706 2 1416 .......................................................................................... 1505 T Q R V L L Q E L L K T G K P V V L V L F T G R P L V L N W Query_1 1707 GCAACGTGTACTTTTGCAGGAATTGTTGAAAACCGGCAAACCGGTGGTATTGGTGTTGTTTACCGGTCGTCCGTTAGTATTGAATTGGGA 1796 2 1506 .......................................................................................... 1595 E Q E N V P A I L N V W F G G S E A A L A I G D V L F G N V Query_1 1797 GCAGGAAAATGTACCTGCTATTCTGAATGTGTGGTTTGGTGGTAGTGAAGCTGCTCTTGCCATTGGTGATGTACTGTTTGGAAATGTAAA 1886 2 1596 .......................................................................................... 1685 N P S G K L T T T F P K S V G Q I P L F Y N H K N T G R P L Query_1 1887 TCCGAGTGGCAAACTTACTACTACTTTTCCGAAGAGTGTAGGACAGATTCCTTTGTTCTATAACCATAAGAATACTGGTCGTCCTTTGCC 1976 2 1686 .......................................................................................... 1775 P Q G A W F Q K F R S N Y L D V D N E P L Y P F G Y G L S Y Query_1 1977 TCAAGGGGCCTGGTTCCAGAAGTTCCGTAGCAATTATCTGGATGTAGATAACGAACCGCTTTATCCGTTTGGATATGGCTTGAGCTATAC 2066 2 1776 .......................................................................................... 1865 T T F S Y S D I T L D K S S M N I N G E I M A T V T V T N T Query_1 2067 TACTTTCTCTTATAGTGATATTACATTGGATAAATCGTCCATGAATATCAATGGAGAGATTATGGCAACTGTAACGGTAACCAATACAGG 2156 2 1866 .......................................................................................... 1955 G K Y D G S E V V Q L Y I R D L I G S V T R P V K E L K G F Query_1 2157 TAAGTATGACGGTTCGGAAGTAGTGCAGCTATATATCCGCGATCTTATAGGCAGTGTAACACGTCCGGTGAAAGAACTGAAAGGCTTTGA 2246 2 1956 .......................................................................................... 2045 E K I F L K A G E S K Q V S F K L T A D M L K F Y N Y N L D Query_1 2247 AAAAATCTTCTTGAAAGCCGGTGAATCCAAACAAGTGTCTTTCAAGTTAACAGCTGATATGTTGAAGTTCTACAATTACAATCTGGATTT 2336 2 2046 .......................................................................................... 2135 2 181 ............ 170 F V C E P G D F E V M I G G D S R D V N K A L F S L Q Query_1 2337 TGTGTGCGAACCGGGTGACTTTGAAGTAATGATAGGTGGTGATAGCCGTGATGTGAATAAGGCCTTATTTTCGCTTCAA 2415 2 2136 ............................................................................... 2214 2 2204 .............. 2191 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 9151234 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 3,568 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115086.120 (APC115086.29-766.P(766).E518Q.pMCSG68)
KSPVDMDRFIDDLMKKMTLEEKIGQLNLPVTGEITTGQAKSSNVAKRIRAGEVGGLFNLKGVERIRDVQKQAVEESRLGIPLLFGMDVIHGYETVFPIPLGLSCTWNMTAIEESARIAAIEASADGICWTFSPMVDVSRDPRWGRVSEGNGEDPFLGAEIARAMVRGYQGKDMSSNDEIMACVKHFALYGASEAGRDYNTVDMSHQRMFNEYMLPYQAAVEEGVGSVMASFNEVDGVPATGNKWLMTDVLRKQWNFDGFVVTDYTGITEMTDHGMGDTQTVAALALNAGVDMDMVSDAFTSTLKKSLEEGKVSVKAVDAACRRILEAKYKLGLFDNPYKYCDITRPKKQIFTKEHRAIARKTASESFVLLKNENSVLPLAKKGTIAVVGPLADSRSNMPGTWSVAAVMNKYPSLIEGLKEVVGGKAKILTAKGSNLMSDAEYEERATMFGRTLHRDNRTDKELLDEALAVAAKSDVIVAALGESSEMSGESSCRTDLEMPDTQRVLLQELLKTGKPVVLVLFTGRPLVLNWEQENVPAILNVWFGGSEAALAIGDVLFGNVNPSGKLTTTFPKSVGQIPLFYNHKNTGRPLPQGAWFQKFRSNYLDVDNEPLYPFGYGLSYTTFSYSDITLDKSSMNINGEIMATVTVTNTGKYDGSEVVQLYIRDLIGSVTRPVKELKGFEKIFLKAGESKQVSFKLTADMLKFYNYNLDFVCEPGDFEVMIGGDSRDVNKALFSLQ
AAATCTCCTGTCGATATGGATCGCTTTATTGATGATCTGATGAAGAAGATGACTCTGGAAGAGAAAATCGGCCAGTTGAACTTGCCTGTTACGGGTGAAATAACCACCGGACAAGCCAAGAGTAGTAATGTGGCTAAGCGTATCCGTGCCGGTGAAGTGGGCGGACTCTTTAACTTGAAAGGCGTGGAGCGTATTCGTGACGTTCAGAAACAGGCAGTAGAAGAAAGTCGTCTGGGTATTCCTCTTTTATTTGGTATGGATGTAATTCATGGATACGAAACGGTATTTCCTATTCCTCTGGGATTATCCTGTACCTGGAACATGACAGCTATTGAAGAATCTGCACGTATTGCTGCTATCGAAGCCAGTGCTGATGGTATTTGCTGGACATTCAGTCCGATGGTGGATGTTTCCCGTGATCCCCGTTGGGGACGAGTTTCCGAAGGGAATGGTGAAGATCCCTTCTTGGGAGCGGAGATTGCGCGTGCTATGGTACGTGGTTATCAAGGGAAAGATATGAGTAGTAATGATGAAATTATGGCTTGCGTGAAGCACTTTGCGTTATATGGGGCATCAGAAGCCGGACGCGACTATAATACAGTGGATATGAGTCATCAACGTATGTTCAACGAATATATGTTACCTTATCAGGCTGCCGTGGAAGAAGGTGTGGGTAGTGTGATGGCTTCATTCAATGAAGTGGATGGTGTACCGGCTACCGGAAATAAGTGGCTGATGACCGATGTACTTCGTAAGCAGTGGAATTTTGATGGGTTCGTTGTGACGGACTATACCGGTATCACTGAAATGACCGATCATGGTATGGGTGATACACAAACAGTTGCAGCCCTGGCTCTGAATGCAGGTGTCGATATGGATATGGTGAGCGATGCTTTTACAAGCACACTTAAAAAATCTCTGGAAGAAGGAAAAGTTTCAGTAAAGGCTGTTGATGCTGCTTGTCGCCGTATTCTGGAAGCTAAGTATAAGCTGGGGCTTTTTGATAATCCCTATAAATATTGTGATATAACCCGTCCTAAAAAACAAATCTTTACAAAAGAACACCGCGCTATAGCCCGTAAGACAGCTTCGGAAAGCTTTGTTCTCTTGAAGAATGAGAATAGTGTACTCCCTCTGGCAAAGAAAGGTACCATTGCTGTAGTAGGTCCTTTGGCCGATAGCCGTAGCAATATGCCGGGCACGTGGAGTGTGGCCGCTGTGATGAACAAATATCCTTCTTTGATTGAAGGCTTGAAAGAAGTAGTGGGAGGCAAGGCTAAAATTCTTACGGCTAAAGGAAGTAATCTGATGAGTGATGCCGAATACGAAGAACGTGCTACTATGTTTGGCCGTACTCTGCATCGTGACAATCGTACAGATAAGGAACTGCTGGATGAGGCGCTTGCTGTAGCTGCCAAGTCTGACGTGATTGTTGCTGCTTTGGGTGAGTCTTCCGAGATGAGCGGTGAAAGTAGTTGCCGTACAGACCTCGAAATGCCGGATACGCAACGTGTACTTTTGCAGGAATTGTTGAAAACCGGCAAACCGGTGGTATTGGTGTTGTTTACCGGTCGTCCGTTAGTATTGAATTGGGAGCAGGAAAATGTACCTGCTATTCTGAATGTGTGGTTTGGTGGTAGTGAAGCTGCTCTTGCCATTGGTGATGTACTGTTTGGAAATGTAAATCCGAGTGGCAAACTTACTACTACTTTTCCGAAGAGTGTAGGACAGATTCCTTTGTTCTATAACCATAAGAATACTGGTCGTCCTTTGCCTCAAGGGGCCTGGTTCCAGAAGTTCCGTAGCAATTATCTGGATGTAGATAACGAACCGCTTTATCCGTTTGGATATGGCTTGAGCTATACTACTTTCTCTTATAGTGATATTACATTGGATAAATCGTCCATGAATATCAATGGAGAGATTATGGCAACTGTAACGGTAACCAATACAGGTAAGTATGACGGTTCGGAAGTAGTGCAGCTATATATCCGCGATCTTATAGGCAGTGTAACACGTCCGGTGAAAGAACTGAAAGGCTTTGAAAAAATCTTCTTGAAAGCCGGTGAATCCAAACAAGTGTCTTTCAAGTTAACAGCTGATATGTTGAAGTTCTACAATTACAATCTGGATTTTGTGTGCGAACCGGGTGACTTTGAAGTAATGATAGGTGGTGATAGCCGTGATGTGAATAAGGCCTTATTTTCGCTTCAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,923 total letters Query= APC115086.120 Length=2619 Score E Sequences producing significant alignments: (Bits) Value wt 3984 0.0 12F_F6_APC115086.120_F06.seq 1355 0.0 06F_F6_APC115086.120_F06.seq 1274 0.0 K S P V D M D R F I D D L M K K M T L E E K I G Q L N L P V Query_1 202 AAATCTCCTGTCGATATGGATCGCTTTATTGATGATCTGATGAAGAAGATGACTCTGGAAGAGAAAATCGGCCAGTTGAACTTGCCTGTT 291 2 1 .......................................................................................... 90 2 867 ............. 879 1 700 ............. 688 T G E I T T G Q A K S S N V A K R I R A G E V G G L F N L K Query_1 292 ACGGGTGAAATAACCACCGGACAAGCCAAGAGTAGTAATGTGGCTAAGCGTATCCGTGCCGGTGAAGTGGGCGGACTCTTTAACTTGAAA 381 2 91 .......................................................................................... 180 2 2095 ........... 2085 2 519 ........... 529 G V E R I R D V Q K Q A V E E S R L G I P L L F G M D V I H Query_1 382 GGCGTGGAGCGTATTCGTGACGTTCAGAAACAGGCAGTAGAAGAAAGTCGTCTGGGTATTCCTCTTTTATTTGGTATGGATGTAATTCAT 471 2 181 .......................................................................................... 270 2 2084 . 2084 G Y E T V F P I P L G L S C T W N M T A I E E S A R I A A I Query_1 472 GGATACGAAACGGTATTTCCTATTCCTCTGGGATTATCCTGTACCTGGAACATGACAGCTATTGAAGAATCTGCACGTATTGCTGCTATC 561 2 271 .......................................................................................... 360 E A S A D G I C W T F S P M V D V S R D P R W G R V S E G Query_1 562 GAAGCCAGTGCTGATGGTATTTGCTGGACATTCAGTCCGATGGTGGATGTTTCCCGTGAT--CCCCGTTGGGGACGAGTTTCCGAAGGGA 649 2 361 ............................................................--............................ 448 1 1246 .....N..N...ATC.....N...... 1220 N G E D P F L G A E I A R A M V R G Y Q G K D M S S N D E I Query_1 650 ATGGTGAAGATCCCTTCTTGGGAGCGGAGATTGCGCGTGCTATGGTACGTGGTTATCAAGGGAAAGATATGAGTAGTAATGATGAAATTA 739 2 449 .......................................................................................... 538 2 120 ........... 130 M A C V K H F A L Y G A S E A G R D Y N T V D M S H Q R M F Query_1 740 TGGCTTGCGTGAAGCACTTTGCGTTATATGGGGCATCAGAAGCCGGACGCGACTATAATACAGTGGATATGAGTCATCAACGTATGTTCA 829 2 539 .......................................................................................... 628 N E Y M L P Y Q A A V E E G V G S V M A S F N E V D G V P Query_1 830 ACGAATATATGTTACCTTATCAGGCTGCCGTGGAAGAAGGTGTGGGTAGTGTGATGGCTTCATTCAATGAAGTGGATG-GTGTACC--GG 916 2 629 ..............................................................................-.......--.. 715 1 886 .....C....T..CG.. 870 A T G N K W L M T D V L R K Q W N F D G F V V T D Y Query_1 917 CTACCGGAAATAAGTGG-CTGATGA--CCGATGT-ACTT--CGTAAGC-AGTGGAATTTT--GATGGGTTCG-TTGTG-ACGGACTA-TA 994 2 716 .................-.......--.......-....--.......-...........--..........-.....-........-.. 793 1 869 ..T..............GNN....NAC.......T....TC.....N.C...N.N.N...TN..........G.....G.......NN.. 780 T G I T E M T D H G M G D T Q T V A A L A L N A G V D M Query_1 995 CC-GGTATC-ACTGAAA-TGACC-GATCATGGTATGGGTGATACACAAACAGTTGCAGCCC-TGGCTCTGAATGCAGG-TGTCGATATGG 1078 2 794 ..-......-.......-.....-.....................................-................-........... 877 2 9 ........... 19 1 779 ..C......C.......A.....C..................N..................C................G........... 690 D M V S D A F T S T L K K S L E E G K V S V K A V D A A C R Query_1 1079 ATATGGTGAGCGATGCTTTTACAAGCACACTTAAAAAATCTCTGGAAGAAGGAAAAGTTTCAGTAAAGGCTGTTGATGCTGCTTGTCGCC 1168 2 878 .......................................................................................... 967 2 20 .. 21 1 689 .......................................................................................N.. 600 R I L E A K Y K L G L F D N P Y K Y C D I T R P K K Q I F T Query_1 1169 GTATTCTGGAAGCTAAGTATAAGCTGGGGCTTTTTGATAATCCCTATAAATATTGTGATATAACCCGTCCTAAAAAACAAATCTTTACAA 1258 2 968 .......................................................................................... 1057 1 599 .......................................................................................... 510 K E H R A I A R K T A S E S F V L L K N E N S V L P L A K K Query_1 1259 AAGAACACCGCGCTATAGCCCGTAAGACAGCTTCGGAAAGCTTTGTTCTCTTGAAGAATGAGAATAGTGTACTCCCTCTGGCAAAGAAAG 1348 2 1058 .......................................................................................... 1147 1 509 .......................................................................................... 420 0 5 ....... 11 G T I A V V G P L A D S R S N M P G T W S V A A V M N K Y P Query_1 1349 GTACCATTGCTGTAGTAGGTCCTTTGGCCGATAGCCGTAGCAATATGCCGGGCACGTGGAGTGTGGCCGCTGTGATGAACAAATATCCTT 1438 2 1148 .......................................................................................... 1237 1 419 .......................................................................................... 330 0 12 ......................................TN.................................................. 101 S L I E G L K E V V G G K A K I L T A K G S N L M S D A E Y Query_1 1439 CTTTGATTGAAGGCTTGAAAGAAGTAGTGGGAGGCAAGGCTAAAATTCTTACGGCTAAAGGAAGTAATCTGATGAGTGATGCCGAATACG 1528 2 1238 .......................................................................................... 1327 1 329 .......................................................................................... 240 0 102 .......................................................................................... 191 E E R A T M F G R T L H R D N R T D K E L L D E A L A V A A Query_1 1529 AAGAACGTGCTACTATGTTTGGCCGTACTCTGCATCGTGACAATCGTACAGATAAGGAACTGCTGGATGAGGCGCTTGCTGTAGCTGCCA 1618 2 1328 .......................................................................................... 1417 1 239 .......................................................................................... 150 0 192 .......................................................................................... 281 K S D V I V A A L G E S S E M S G Q S S C R T D L E M P D T Query_1 1619 AGTCTGACGTGATTGTTGCTGCTTTGGGTGAGTCTTCCGAGATGAGCGGTCAGAGTAGTTGCCGTACAGACCTCGAAATGCCGGATACGC 1708 2 1418 ..................................................G.A..................................... 1507 1 149 ..........................................................................N............... 60 0 282 .......................................................................................... 371 Q R V L L Q E L L K T G K P V V L V L F T G R P L V L N Query_1 1709 AACGTGTACTTTTGCAGG-AATTGTTGAAAA--CCGGCAAA--CCGGTGGTATTGGTGTTGTTTACCGGTCGTCCGTTAGTATTGAATTG 1793 2 1508 ..................-............--........--............................................... 1592 1 59 ..................A............NC........CC.......... 7 0 372 ..................-............--........--............................................... 456 W E Q E N V P A I L N V W F G G S E A A L A I G D V L F G N Query_1 1794 GGAGCAGGAAAATGTACCTGCTATTCTGAATGTGTGGTTTGGTGGTAGTGAAGCTGCTCTTGCCATTGGTGATGTACTGTTTGGAAATGT 1883 2 1593 .......................................................................................... 1682 0 457 .........................................N................................................ 546 V N P S G K L T T T F P K S V G Q I P L F Y N H K N T G R P Query_1 1884 AAATCCGAGTGGCAAACTTACTACTAC-TTTTCCGAAGAGTGTAGGACAGATTCCTTTGTTCTATAACCATAAGAATACTGGTCGTCCTT 1972 2 1683 ...........................-.............................................................. 1771 0 547 ......N....................T................N............................N................ 636 L P Q G A W F Q K F R S N Y L D V D N E P L Y P F G Y G L Query_1 1973 TGCCTCAAGGGGCCTGGTTCCAGAAGTTCCGT-AGCAATTATCTGGATGTAGATAACGAACCGC-TTTATCCGTTTGGATATGGCTTG-A 2059 2 1772 ................................-...............................-.......................-. 1858 0 637 ..........N.....................A.N..................N...N......T..............N........A. 726 S Y T T F S Y S D I T L D K S S M N I N G E I M A T Query_1 2060 GCTATA-CTACTTTCT-CTTATAG-TGAT-ATTACATT-GGAT-AAATC-GTCCATGAATATCAA-TGGA-GAGATTATGG-CAACTG-- 2137 2 1859 ......-.........-.......-....-........-....-.....-...............-....-..........-......-- 1936 0 727 N.....C.........C......NC....A.....N..N...NA.....NN...N.......NN.T....T.......N.NC....CNGC 816 V T V T N T G K Y D G S E V V Q L Y I R D L I G S V T R P V Query_1 2138 TAACGG-TAACCAATACAGGTAAGTATGACGGTTCGGAAGTAGTGCAGCTATATATCCGCGATCTTATAGGCAGTGTAACACGTCCGGTG 2226 2 1937 ......-................................................................................... 2025 0 817 ......NN......A..... 836 K E L K G F E K I F L K A G E S K Q V S F K L T A D M L K F Query_1 2227 AAAGAACTGAAAGGCTTTGAAAAAATCTTCTTGAAAGCCGGTGAATCCAAACAAGTGTCTTTCAAGTTAACAGCTGATATGTTGAAGTTC 2316 2 2026 .......................................................................................... 2115 2 181 ............ 170 Y N Y N L D F V C E P G D F E V M I G G D S R D V N K A L F Query_1 2317 TACAATTACAATCTGGATTTTGTGTGCGAACCGGGTGACTTTGAAGTAATGATAGGTGGTGATAGCCGTGATGTGAATAAGGCCTTATTT 2406 2 2116 .......................................................................................... 2205 2 2204 .............. 2191 S L Q Query_1 2407 TCGCTTCAA 2415 2 2206 ......... 2214 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12676944 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 4,923 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200018.105 (CMR200018.20-949.P(949).E413A.pMCSG68)
QTLPQTERQYLSGHGCDDTVEWDFFCTDGRNSGRWTKIGVPSCWELQGFGTYQYGISFYGKAFPEGIAGEKGMYKYEFEVPEEFRGKQVSLVFEASMTDTEVKVNGRKAGSKHQGAFYCFSYNVTDLLKYGKKNQLEVTVSKESENASVNLAERRADYWNFGGIFRPVFLEVKPAVNLRHIAIDAQMDGSFRANCYTNISGDGMSIRAQILDGKGKKLADTTVPLKAGSDWTTLQLNVSAPALWTAETPNLYKAQFSLLDKGGKVLHHETETFGFRTIEVRESDGLYVNGVRINVRGVNRHSFRPESGRTLSKAKNIEDVLLMKGMNMNSVRLSHYPADPEFLEACDSLGLYVMDELGGWHGKYDTPTGVRLIEGMIERDVNHPSIIWWSNGNEKGWNIELDGEFHKYDLQKRPVIHPQGNFSGFETMHYRSYGESQNYMRLPEIFMPTEFLHGLYDGGHGAGLYDYWEMMRKHPRCIGGFLWVLADEGVKRVDMDGFIDNQGNFGADGIVGPHHEKEGSYYTIKQLWSPVQVMNTAIDRNFDGKLSVENRYDYLNLNTCRFIWQQVKFPSVTDASNTTTRILKQGEVQGSDVAAHGVGVVDIKTSILPEADALFLTVIDKYGYELWRWTFPVDKLNRETEQFSASSGRVSYTETEKGITVKANGRTFVFSKKDGQLKDVSVNNRKISFANGPRFIGARRADRSLDQFYNHDDEKAKAKDRTYSEFTDAAVFTKLDVKEEGGNLILTANYKLGNLDKAQWTIHPDGMATLDYTYNFSGVVDLMGICFDYPEEQVLSKRWLGAGPYRVWQNRIHGTQYDIWENDYNDPIPGETFTYPEFKGYFGSVSWMSIRTKEGTISLTNETPDSYIGVYQPRDGRDRLLYTLPESGISVLNVIPPVRNKVNSTDLCGPSSQPKWVDGSQTGRLVIRFE
CAGACACTTCCGCAGACAGAGCGGCAATACCTCTCCGGCCACGGATGCGACGACACAGTAGAATGGGACTTTTTCTGTACCGACGGACGTAACTCCGGTCGATGGACGAAAATAGGCGTCCCCTCTTGCTGGGAGTTGCAGGGTTTTGGTACCTATCAGTATGGAATTAGTTTTTATGGTAAAGCCTTTCCCGAAGGCATTGCCGGTGAGAAAGGAATGTATAAATATGAGTTTGAAGTTCCCGAGGAATTTCGTGGCAAGCAGGTCAGCCTTGTGTTCGAAGCATCCATGACCGATACGGAAGTTAAGGTTAACGGACGTAAGGCAGGATCGAAACACCAGGGAGCCTTCTATTGCTTTTCATATAATGTCACGGATTTACTGAAATATGGCAAGAAGAATCAGCTGGAAGTAACAGTTTCCAAGGAGAGTGAGAATGCCAGTGTGAATCTTGCCGAACGGCGCGCCGATTATTGGAACTTTGGCGGTATCTTCCGCCCGGTATTTCTGGAAGTAAAACCTGCCGTCAATCTCCGTCATATTGCTATTGATGCACAAATGGACGGATCATTCCGTGCCAATTGCTACACGAATATCTCCGGTGACGGAATGAGTATCCGTGCACAGATTTTGGACGGTAAAGGGAAGAAACTGGCAGATACCACCGTACCCCTAAAAGCCGGAAGCGACTGGACTACTTTACAATTGAACGTTTCTGCCCCTGCCTTATGGACGGCAGAAACTCCGAATCTTTATAAAGCTCAATTTTCACTGTTGGATAAAGGAGGTAAAGTCCTGCATCATGAGACCGAGACATTCGGTTTCCGTACTATCGAAGTTCGTGAAAGTGACGGATTGTACGTGAACGGGGTGCGTATCAACGTGCGTGGTGTCAACCGTCATAGTTTCCGTCCCGAAAGCGGTCGTACCCTAAGTAAAGCGAAGAATATTGAAGATGTACTTCTGATGAAGGGCATGAATATGAATTCTGTCCGTCTGAGCCACTATCCGGCGGACCCGGAATTTCTGGAAGCATGCGACTCTCTTGGACTCTATGTTATGGATGAACTGGGTGGCTGGCATGGCAAGTACGACACCCCTACGGGAGTACGTCTGATTGAAGGCATGATAGAACGTGATGTGAACCATCCGTCCATTATCTGGTGGAGCAATGGTAATGAAAAAGGCTGGAACATTGAACTGGACGGAGAATTCCATAAATACGATCTGCAGAAACGCCCGGTCATCCATCCGCAAGGTAACTTCTCCGGTTTCGAAACCATGCACTATCGTTCGTATGGAGAAAGCCAGAACTACATGCGCCTGCCGGAAATCTTTATGCCTACTGAATTCCTGCATGGTTTGTACGACGGAGGTCATGGTGCCGGCCTGTATGATTACTGGGAAATGATGCGTAAACATCCGCGTTGTATCGGTGGTTTCCTGTGGGTATTGGCGGATGAAGGCGTGAAGCGCGTGGATATGGACGGGTTCATAGACAATCAGGGAAATTTCGGAGCTGACGGAATTGTAGGCCCTCACCATGAAAAGGAAGGCAGCTATTACACTATCAAGCAGCTATGGAGCCCGGTGCAGGTTATGAATACCGCTATCGACCGGAATTTCGACGGTAAACTCTCTGTGGAGAACCGTTATGATTATCTGAACCTGAACACCTGTCGTTTTATCTGGCAGCAAGTGAAGTTCCCGTCGGTAACGGATGCTTCCAATACAACTACACGGATTCTGAAACAAGGTGAAGTGCAAGGAAGCGATGTAGCAGCCCATGGAGTGGGAGTGGTGGATATCAAGACTTCTATTCTTCCCGAAGCGGATGCTCTTTTCCTGACAGTTATAGATAAATATGGGTATGAACTTTGGCGCTGGACTTTCCCCGTAGATAAACTGAATCGGGAAACAGAACAGTTTTCTGCATCATCCGGCCGTGTATCCTATACGGAAACAGAAAAAGGTATTACGGTAAAAGCAAACGGGCGTACTTTTGTCTTTTCAAAGAAAGACGGGCAGCTGAAAGATGTATCCGTCAATAACCGTAAGATTAGTTTTGCTAACGGTCCCCGTTTTATCGGTGCACGTCGTGCAGACCGTTCCCTAGATCAGTTCTATAATCATGATGACGAAAAAGCCAAGGCAAAGGACCGTACTTACAGTGAATTTACCGATGCGGCAGTCTTCACGAAACTGGATGTGAAAGAAGAGGGGGGGAATCTGATCCTCACCGCTAATTATAAACTGGGTAATTTAGATAAAGCTCAGTGGACAATTCATCCGGACGGCATGGCTACTCTTGATTATACCTACAACTTCTCCGGTGTGGTAGACCTGATGGGTATTTGCTTTGATTACCCTGAAGAACAAGTGCTCAGCAAGCGTTGGTTGGGAGCAGGTCCGTATCGTGTATGGCAGAATCGTATTCATGGCACGCAGTATGATATCTGGGAGAATGATTATAACGATCCTATTCCGGGTGAGACATTCACCTATCCTGAATTCAAGGGATATTTTGGCAGTGTCTCTTGGATGAGTATTCGCACGAAAGAGGGAACCATCAGCCTGACGAATGAAACACCTGATTCCTATATCGGAGTATATCAACCCCGTGATGGTCGTGACCGGTTACTGTATACACTTCCCGAAAGCGGAATTTCTGTTTTGAATGTAATTCCTCCGGTGCGTAATAAAGTAAATTCCACGGACTTGTGCGGTCCTTCTTCACAACCAAAATGGGTGGATGGCTCGCAAACGGGACGCCTTGTTATCCGGTTTGAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,549 total letters Query= CMR200018.105 Length=3195 Score E Sequences producing significant alignments: (Bits) Value wt 5023 0.0 06G_G6_CMR200018.105_G06.seq 948 0.0 12G_G6_CMR200018.105_G06.seq 827 0.0 Q T L P Q T E R Q Y L S G H G C D D T V E W D F F C T D G R Query_1 202 CAGACACTTCCGCAGACAGAGCGGCAATACCTCTCCGGCCACGGATGCGACGACACAGTAGAATGGGACTTTTTCTGTACCGACGGACGT 291 2 1 .......................................................................................... 90 N S G R W T K I G V P S C W E L Q G F G T Y Q Y G I S F Y G Query_1 292 AACTCCGGTCGATGGACGAAAATAGGCGTCCCCTCTTGCTGGGAGTTGCAGGGTTTTGGTACCTATCAGTATGGAATTAGTTTTTATGGT 381 2 91 .......................................................................................... 180 K A F P E G I A G E K G M Y K Y E F E V P E E F R G K Q V S Query_1 382 AAAGCCTTTCCCGAAGGCATTGCCGGTGAGAAAGGAATGTATAAATATGAGTTTGAAGTTCCCGAGGAATTTCGTGGCAAGCAGGTCAGC 471 2 181 .......................................................................................... 270 L V F E A S M T D T E V K V N G R K A G S K H Q G A F Y C F Query_1 472 CTTGTGTTCGAAGCATCCATGACCGATACGGAAGTTAAGGTTAACGGACGTAAGGCAGGATCGAAACACCAGGGAGCCTTCTATTGCTTT 561 2 271 .......................................................................................... 360 S Y N V T D L L K Y G K K N Q L E V T V S K E S E N A S V N Query_1 562 TCATATAATGTCACGGATTTACTGAAATATGGCAAGAAGAATCAGCTGGAAGTAACAGTTTCCAAGGAGAGTGAGAATGCCAGTGTGAAT 651 2 361 .......................................................................................... 450 L A E R R A D Y W N F G G I F R P V F L E V K P A V N L R H Query_1 652 CTTGCCGAACGGCGCGCCGATTATTGGAACTTTGGCGGTATCTTCCGCCCGGTATTTCTGGAAGTAAAACCTGCCGTCAATCTCCGTCAT 741 2 451 .......................................................................................... 540 2 1017 .....A........... 1033 2 1873 ........... 1883 1 426 ...N.A........... 410 I A I D A Q M D G S F R A N C Y T N I S G D G M S I R A Q I Query_1 742 ATTGCTATTGATGCACAAATGGACGGATCATTCCGTGCCAATTGCTACACGAATATCTCCGGTGACGGAATGAGTATCCGTGCACAGATT 831 2 541 .......................................................................................... 630 1 284 ........... 294 L D G K G K K L A D T T V P L K A G S D W T T L Q L N V S A Query_1 832 TTGGACGGTAAAGGGAAGAAACTGGCAGATACCACCGTACCCCTAAAAGCCGGAAGCGACTGGACTACTTTACAATTGAACGTTTCTGCC 921 2 631 .......................................................................................... 720 P A L W T A E T P N L Y K A Q F S L L D K G G K V L H Query_1 922 CCTGCCTTATGGACGGCAG-AAACTCC-GAATCTTTATAAA-GCTCAATTTTCACTGTTG-GATAAA-GGAGGTA-AAGT-CCTGCA-TC 1003 2 721 ...................-.......-.............-..................-......-.......-....-......-.. 802 1 721 .....G.N...N.N.....N.......A.N.....GN..G.A.G..TN....NT.......N....N.....NN.. 646 H E T E T F G F R T I E V R E S D G L Y V N G V R I N V Query_1 1004 ATGAGACCGA-GACATTCGGTTTCCG--TACTATCGAAGTTCGTGAAAGTGACGGATTGT--ACGTGAACGGGGTGCGTATCAACGTGCG 1088 2 803 ..........-...............--................................--............................ 887 1 645 .N..NNT.NNC............G.NNN......A...T..GN.N....N.N...NAG.GNA.........A..............N..N 556 R G V N R H S F R P E S G R T L S K A K N I E D V L L M K G Query_1 1089 TGGTGTCAACCGTCATAGTTTCCGTCCCGAAAGCGGTCGTACCCTAAGTAAAGCGAAGAATATTGAAGATGTACTTCTGATGAAGGGCAT 1178 2 888 .......................................................................................... 977 2 2652 ............ 2663 1 555 N......NN................T.N............N..N.................N...NG.......N.G....N........ 466 M N M N S V R L S H Y P A D P E F L E A C D S L G L Y V M D Query_1 1179 GAATATGAATTCTGTCCGTCTGAGCCACTATCCGGCGGACCCGGAATTTCTGGAAGCATGCGACTCTCTTGGACTCTAT-GTTATGGATG 1267 2 978 ...............................................................................-.......... 1066 2 498 .....T........... 514 2 2661 ........... 2671 1 465 .............N............G.G.........N...N....................................C.......... 376 E L G G W H G K Y D T P T G V R L I E G M I E R D V N H P S Query_1 1268 AACTGGGTGGCTGGCATGGCAAGTACGACACCCCTACGGGAGTACGTCTGATTGAAGGCATGATAGAACGTGATGTGAACCATCCGTCCA 1357 2 1067 .......................................................................................... 1156 2 568 ......... 560 1 375 ...N..............N..........................N............................................ 286 I I W W S N G N A K G W N I E L D G E F H K Y D L Q K R P V Query_1 1358 TTATCTGGTGGAGCAATGGTAATGCTAAAGGCTGGAACATTGAACTGGACGGAGAATTCCATAAATACGATCTGCAGAAACGCCCGGT-C 1446 2 1157 ........................AA..............................................................-. 1245 2 559 .. 558 0 1 ........N........................................NT.TN 54 1 285 ..........N................................N..N........................N................-. 197 I H P Q G N F S G F E T M H Y R S Y G E S Q N Y M R L P E Query_1 1447 ATCCATCCGCAAGGTAACTTCTCCGGTTTCGAAACCATGCACTATCGT-TCGTATGGAGAAAGCCAGAACTACATGCGCCTGCCGGAAAT 1535 2 1246 ................................................-......................................... 1334 2 1281 ............ 1270 2 2323 ............ 2334 0 55 ..............A.............................N...C......................................... 144 1 196 .............................N..........N.......-...................C...............NN..N. 108 I F M P T E F L H G L Y D G G H G A G L Y D Y W E M M R K Query_1 1536 CTTTATGCCTAC-TGAATTCCTGCA-TGGTTTGTA-CGACGGAGGT-CATGGTGCC-GGCCTGTATGATTACTGGGAAATGATGCGTAAA 1620 2 1335 ............-............-.........-..........-.........-................................. 1419 0 145 ............-............-.........-..........-.....N...-................................. 229 1 107 ..........N.G........N...N.........G.N........A.....C...G...N.....ANN.T...NN...CN......... 18 H P R C I G G F L W V L A D E G V K R V D M D G F I D N Q G Query_1 1621 CATCCGCGTTGTATCGGTGGTTTCCTGTGGGTATTGGCGGATGAAGGCGTGAAGCGCGTGGATATGGACGGGTTCATAGACAATCAGGGA 1710 2 1420 .......................................................................................... 1509 0 230 .......................................................................................... 319 1 17 .-..... 12 N F G A D G I V G P H H E K E G S Y Y T I K Q L W S P V Q V Query_1 1711 AATTTCGGAGCTGACGGAATTGTAGGCCCTCACCATGAAAAGGAAGGCAGCTATTACACTATCAAGCAGCTATGGAGCCCGGTGCAGGTT 1800 2 1510 .......................................................................................... 1599 0 320 ...................N..............................N......N.....N......................N... 409 M N T A I D R N F D G K L S V E N R Y D Y L N L N T C R F Query_1 1801 ATGAATACCGCTATCGACCGGAATTTCGACGGTAAACTCTCTGTGGAGAACCG-TTATGATTATCTGAACCTGAACACCTGTCGTTTTAT 1889 2 1600 .....................................................-.................................... 1688 0 410 .....................................................NN..C.......................C..N..... 499 I W Q Q V K F P S V T D A S N T T T R I L K Q G E V Q G S D Query_1 1890 CTGGCAGCAAGTGAAG-TTCCCGTCGGTAACGGATGCTTCCAATACAACTACACGGATTCTGAAACAAGGTG-AAGTGCAAGGAAGCGAT 1977 2 1689 ................-.......................................................-................. 1776 2 234 .....-...... 244 0 500 ....N...........T......N.N..N.....N......N...NC.....NN.........N..NN....A..N...N.......... 589 V A A H G V G V V D I K T S I L P E A D A L F L T V Query_1 1978 -GTAGCAGCCCATGG-AGTGGGAGTGGTGGATATC-AAGAC-TTCTATT-CTTCCC--GAAG-CGG-ATG-CTC-TTTTCCTGA-CAGTT 2055 2 1777 -..............-...................-.....-.......-......--....-...-...-...-.........-..... 1854 0 590 NNN.T..T.N..C..A..N.NN.....N.......N.....T...N...C......CG...NA...C...C...N..N...C.NC....N 679 I D K Y G Y E L W R W T F P V D K L N R E T E Q F S A Query_1 2056 -ATAGATAAATATGGG---TATGAAC-TTTGGCGCT-GGACTTTCC-CCGTAGATAAACTGAATCGGGAAACAGAACAGTTTTCTGCATC 2138 2 1855 -...............---.......-.........-.........-........................................... 1937 2 477 ....-....... 487 0 680 A............N..TGT.......T........NG..C......N..N.... 733 S S G R V S Y T E T E K G I T V K A N G R T F V F S K K D G Query_1 2139 ATCCGGCCGTGTATCCTATACGGAAACAGAAAAAGGTATTACGGTAAAAGCAAACGGGCGTACTTTTGTCTTTTCAAAGAAAGACGGGCA 2228 2 1938 .......................................................................................... 2027 Q L K D V S V N N R K I S F A N G P R F I G A R R A D R S L Query_1 2229 GCTGAAAGATGTATCCGTCAATAACCGTAAGATTAGTTTTGCTAACGGTCCCCGTTTTATCGGTGCACGTCGTGCAGACCGTTCCCTAGA 2318 2 2028 .......................................................................................... 2117 D Q F Y N H D D E K A K A K D R T Y S E F T D A A V F T K L Query_1 2319 TCAGTTCTATAATCATGATGACGAAAAAGCCAAGGCAAAGGACCGTACTTACAGTGAATTTACCGATGCGGCAGTCTTCACGAAACTGGA 2408 2 2118 .......................................................................................... 2207 D V K E E G G N L I L T A N Y K L G N L D K A Q W T I H P D Query_1 2409 TGTGAAAGAAGAgggggggAATCTGATCCTCACCGCTAATTATAAACTGGGTAATTTAGATAAAGCTCAGTGGACAATTCATCCGGACGG 2498 2 2208 .......................................................................................... 2297 G M A T L D Y T Y N F S G V V D L M G I C F D Y P E E Q V L Query_1 2499 CATGGCTACTCTTGATTATACCTACAACTTCTCCGGTGTGGTAGACCTGATGGGTATTTGCTTTGATTACCCTGAAGAACAAGTGCTCAG 2588 2 2298 .......................................................................................... 2387 2 1261 ............ 1272 0 70 ............ 81 1 181 ............ 170 S K R W L G A G P Y R V W Q N R I H G T Q Y D I W E N D Y N Query_1 2589 CAAGCGTTGGTTGGGAGCAGGTCCGTATCGTGTATGGCAGAATCGTATTCATGGCACGCAGTATGATATCTGGGAGAATGATTATAACGA 2678 2 2388 .......................................................................................... 2477 D P I P G E T F T Y P E F K G Y F G S V S W M S I R T K E G Query_1 2679 TCCTATTCCGGGTGAGACATTCACCTATCCTGAATTCAAGGGATATTTTGGCAGTGTCTCTTGGATGAGTATTCGCACGAAAGAGGGAAC 2768 2 2478 .......................................................................................... 2567 T I S L T N E T P D S Y I G V Y Q P R D G R D R L L Y T L P Query_1 2769 CATCAGCCTGACGAATGAAACACCTGATTCCTATATCGGAGTATATCAACCCCGTGATGGTCGTGACCGGTTACTGTATACACTTCCCGA 2858 2 2568 .......................................................................................... 2657 2 912 ...... 917 E S G I S V L N V I P P V R N K V N S T D L C G P S S Q P K Query_1 2859 AAGCGGAATTTCTGTTTTGAATGTAATTCCTCCGGTGCGTAATAAAGTAAATTCCACGGACTTGTGCGGTCCTTCTTCACAACCAAAATG 2948 2 2658 .......................................................................................... 2747 2 918 ...... 923 2 1019 ........... 1029 W V D G S Q T G R L V I R F E Query_1 2949 GGTGGATGGCTCGCAAACGGGACGCCTTGTTATCCGGTTTGAA 2991 2 2748 ........................................... 2790 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 17457615 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 5,549 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200018.107 (CMR200018.20-949.P(949).E469A.pMCSG68)
QTLPQTERQYLSGHGCDDTVEWDFFCTDGRNSGRWTKIGVPSCWELQGFGTYQYGISFYGKAFPEGIAGEKGMYKYEFEVPEEFRGKQVSLVFEASMTDTEVKVNGRKAGSKHQGAFYCFSYNVTDLLKYGKKNQLEVTVSKESENASVNLAERRADYWNFGGIFRPVFLEVKPAVNLRHIAIDAQMDGSFRANCYTNISGDGMSIRAQILDGKGKKLADTTVPLKAGSDWTTLQLNVSAPALWTAETPNLYKAQFSLLDKGGKVLHHETETFGFRTIEVRESDGLYVNGVRINVRGVNRHSFRPESGRTLSKAKNIEDVLLMKGMNMNSVRLSHYPADPEFLEACDSLGLYVMDELGGWHGKYDTPTGVRLIEGMIERDVNHPSIIWWSNGNEKGWNIELDGEFHKYDLQKRPVIHPQGNFSGFETMHYRSYGESQNYMRLPEIFMPTEFLHGLYDGGHGAGLYDYWEMMRKHPRCIGGFLWVLADEGVKRVDMDGFIDNQGNFGADGIVGPHHEKEGSYYTIKQLWSPVQVMNTAIDRNFDGKLSVENRYDYLNLNTCRFIWQQVKFPSVTDASNTTTRILKQGEVQGSDVAAHGVGVVDIKTSILPEADALFLTVIDKYGYELWRWTFPVDKLNRETEQFSASSGRVSYTETEKGITVKANGRTFVFSKKDGQLKDVSVNNRKISFANGPRFIGARRADRSLDQFYNHDDEKAKAKDRTYSEFTDAAVFTKLDVKEEGGNLILTANYKLGNLDKAQWTIHPDGMATLDYTYNFSGVVDLMGICFDYPEEQVLSKRWLGAGPYRVWQNRIHGTQYDIWENDYNDPIPGETFTYPEFKGYFGSVSWMSIRTKEGTISLTNETPDSYIGVYQPRDGRDRLLYTLPESGISVLNVIPPVRNKVNSTDLCGPSSQPKWVDGSQTGRLVIRFE
CAGACACTTCCGCAGACAGAGCGGCAATACCTCTCCGGCCACGGATGCGACGACACAGTAGAATGGGACTTTTTCTGTACCGACGGACGTAACTCCGGTCGATGGACGAAAATAGGCGTCCCCTCTTGCTGGGAGTTGCAGGGTTTTGGTACCTATCAGTATGGAATTAGTTTTTATGGTAAAGCCTTTCCCGAAGGCATTGCCGGTGAGAAAGGAATGTATAAATATGAGTTTGAAGTTCCCGAGGAATTTCGTGGCAAGCAGGTCAGCCTTGTGTTCGAAGCATCCATGACCGATACGGAAGTTAAGGTTAACGGACGTAAGGCAGGATCGAAACACCAGGGAGCCTTCTATTGCTTTTCATATAATGTCACGGATTTACTGAAATATGGCAAGAAGAATCAGCTGGAAGTAACAGTTTCCAAGGAGAGTGAGAATGCCAGTGTGAATCTTGCCGAACGGCGCGCCGATTATTGGAACTTTGGCGGTATCTTCCGCCCGGTATTTCTGGAAGTAAAACCTGCCGTCAATCTCCGTCATATTGCTATTGATGCACAAATGGACGGATCATTCCGTGCCAATTGCTACACGAATATCTCCGGTGACGGAATGAGTATCCGTGCACAGATTTTGGACGGTAAAGGGAAGAAACTGGCAGATACCACCGTACCCCTAAAAGCCGGAAGCGACTGGACTACTTTACAATTGAACGTTTCTGCCCCTGCCTTATGGACGGCAGAAACTCCGAATCTTTATAAAGCTCAATTTTCACTGTTGGATAAAGGAGGTAAAGTCCTGCATCATGAGACCGAGACATTCGGTTTCCGTACTATCGAAGTTCGTGAAAGTGACGGATTGTACGTGAACGGGGTGCGTATCAACGTGCGTGGTGTCAACCGTCATAGTTTCCGTCCCGAAAGCGGTCGTACCCTAAGTAAAGCGAAGAATATTGAAGATGTACTTCTGATGAAGGGCATGAATATGAATTCTGTCCGTCTGAGCCACTATCCGGCGGACCCGGAATTTCTGGAAGCATGCGACTCTCTTGGACTCTATGTTATGGATGAACTGGGTGGCTGGCATGGCAAGTACGACACCCCTACGGGAGTACGTCTGATTGAAGGCATGATAGAACGTGATGTGAACCATCCGTCCATTATCTGGTGGAGCAATGGTAATGAAAAAGGCTGGAACATTGAACTGGACGGAGAATTCCATAAATACGATCTGCAGAAACGCCCGGTCATCCATCCGCAAGGTAACTTCTCCGGTTTCGAAACCATGCACTATCGTTCGTATGGAGAAAGCCAGAACTACATGCGCCTGCCGGAAATCTTTATGCCTACTGAATTCCTGCATGGTTTGTACGACGGAGGTCATGGTGCCGGCCTGTATGATTACTGGGAAATGATGCGTAAACATCCGCGTTGTATCGGTGGTTTCCTGTGGGTATTGGCGGATGAAGGCGTGAAGCGCGTGGATATGGACGGGTTCATAGACAATCAGGGAAATTTCGGAGCTGACGGAATTGTAGGCCCTCACCATGAAAAGGAAGGCAGCTATTACACTATCAAGCAGCTATGGAGCCCGGTGCAGGTTATGAATACCGCTATCGACCGGAATTTCGACGGTAAACTCTCTGTGGAGAACCGTTATGATTATCTGAACCTGAACACCTGTCGTTTTATCTGGCAGCAAGTGAAGTTCCCGTCGGTAACGGATGCTTCCAATACAACTACACGGATTCTGAAACAAGGTGAAGTGCAAGGAAGCGATGTAGCAGCCCATGGAGTGGGAGTGGTGGATATCAAGACTTCTATTCTTCCCGAAGCGGATGCTCTTTTCCTGACAGTTATAGATAAATATGGGTATGAACTTTGGCGCTGGACTTTCCCCGTAGATAAACTGAATCGGGAAACAGAACAGTTTTCTGCATCATCCGGCCGTGTATCCTATACGGAAACAGAAAAAGGTATTACGGTAAAAGCAAACGGGCGTACTTTTGTCTTTTCAAAGAAAGACGGGCAGCTGAAAGATGTATCCGTCAATAACCGTAAGATTAGTTTTGCTAACGGTCCCCGTTTTATCGGTGCACGTCGTGCAGACCGTTCCCTAGATCAGTTCTATAATCATGATGACGAAAAAGCCAAGGCAAAGGACCGTACTTACAGTGAATTTACCGATGCGGCAGTCTTCACGAAACTGGATGTGAAAGAAGAGGGGGGGAATCTGATCCTCACCGCTAATTATAAACTGGGTAATTTAGATAAAGCTCAGTGGACAATTCATCCGGACGGCATGGCTACTCTTGATTATACCTACAACTTCTCCGGTGTGGTAGACCTGATGGGTATTTGCTTTGATTACCCTGAAGAACAAGTGCTCAGCAAGCGTTGGTTGGGAGCAGGTCCGTATCGTGTATGGCAGAATCGTATTCATGGCACGCAGTATGATATCTGGGAGAATGATTATAACGATCCTATTCCGGGTGAGACATTCACCTATCCTGAATTCAAGGGATATTTTGGCAGTGTCTCTTGGATGAGTATTCGCACGAAAGAGGGAACCATCAGCCTGACGAATGAAACACCTGATTCCTATATCGGAGTATATCAACCCCGTGATGGTCGTGACCGGTTACTGTATACACTTCCCGAAAGCGGAATTTCTGTTTTGAATGTAATTCCTCCGGTGCGTAATAAAGTAAATTCCACGGACTTGTGCGGTCCTTCTTCACAACCAAAATGGGTGGATGGCTCGCAAACGGGACGCCTTGTTATCCGGTTTGAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,444 total letters Query= CMR200018.107 Length=3195 Score E Sequences producing significant alignments: (Bits) Value wt 5023 0.0 06H_H6_CMR200018.107_H06.seq 1406 0.0 12H_H6_CMR200018.107_H06.seq 1328 0.0 Q T L P Q T E R Q Y L S G H G C D D T V E W D F F C T D G R Query_1 202 CAGACACTTCCGCAGACAGAGCGGCAATACCTCTCCGGCCACGGATGCGACGACACAGTAGAATGGGACTTTTTCTGTACCGACGGACGT 291 2 1 .......................................................................................... 90 N S G R W T K I G V P S C W E L Q G F G T Y Q Y G I S F Y G Query_1 292 AACTCCGGTCGATGGACGAAAATAGGCGTCCCCTCTTGCTGGGAGTTGCAGGGTTTTGGTACCTATCAGTATGGAATTAGTTTTTATGGT 381 2 91 .......................................................................................... 180 K A F P E G I A G E K G M Y K Y E F E V P E E F R G K Q V S Query_1 382 AAAGCCTTTCCCGAAGGCATTGCCGGTGAGAAAGGAATGTATAAATATGAGTTTGAAGTTCCCGAGGAATTTCGTGGCAAGCAGGTCAGC 471 2 181 .......................................................................................... 270 0 510 ........... 520 L V F E A S M T D T E V K V N G R K A G S K H Q G A F Y C F Query_1 472 CTTGTGTTCGAAGCATCCATGACCGATACGGAAGTTAAGGTTAACGGACGTAAGGCAGGATCGAAACACCAGGGAGCCTTCTATTGCTTT 561 2 271 .......................................................................................... 360 S Y N V T D L L K Y G K K N Q L E V T V S K E S E N A S V N Query_1 562 TCATATAATGTCACGGATTTACTGAAATATGGCAAGAAGAATCAGCTGGAAGTAACAGTTTCCAAGGAGAGTGAGAATGCCAGTGTGAAT 651 2 361 .......................................................................................... 450 L A E R R A D Y W N F G G I F R P V F L E V K P A V N L R H Query_1 652 CTTGCCGAACGGCGCGCCGATTATTGGAACTTTGGCGGTATCTTCCGCCCGGTATTTCTGGAAGTAAAACCTGCCGTCAATCTCCGTCAT 741 2 451 .......................................................................................... 540 2 1017 .....A........... 1033 2 1873 ........... 1883 1 424 .....A........... 408 I A I D A Q M D G S F R A N C Y T N I S G D G M S I Query_1 742 ATTGCTATTGATGCACAAATGGACGGATCATTCC-GTGCC-AATTGCTACAC---GAATATCTCCGG-TGAC--GGAAT-GAGTAT--CC 820 2 541 ..................................-.....-...........---............-....--.....-......--.. 619 1 901 .......C.....C.....T.NNN.AGG....N.......GN.N.CG.....T......TC.. 839 R A Q I L D G K G K K L A D T T V P L K A G S D W T T Query_1 821 GTGCACAGATTTTGG--ACGGTAAAGGGAAGAAA-CTGG-CAGATACCACCGTACCCCT-AAAAGCCGG-AAGCG-ACT-GGACTACTTT 902 2 620 ...............--.................-....-...................-.........-.....-...-.......... 701 1 838 .N.............GAN.............N..N....T.....N.........N...T.........G...N.G...T.......... 749 L Q L N V S A P A L W T A E T P N L Y K A Q F S L L D Query_1 903 ACAATTGAA-CGTTT-CTGCCC-CTGCC-TTATGGAC--GGCAGAAACTCCG-AATCTTT-ATAAAGCTCAATTTT-CACTGTTGGATAA 983 2 702 .........-.....-......-.....-........--.............-.......-...............-............. 782 1 748 .........A.....T......N.....G.......ACG............NN.......T...............T............. 659 K G G K V L H H E T E T F G F R T I E V R E S D G L Y V N G Query_1 984 AGGAGGTAAAGTCCTGCATCATGAGACCGAGACATTCGGTTTCCGTACTATCGAAGTTCGTGAAAGTGACGGATTGTACGTGAACGGGGT 1073 2 783 .......................................................................................... 872 1 658 .......................................................................................... 569 V R I N V R G V N R H S F R P E S G R T L S K A K N I E D V Query_1 1074 GCGTATCAACGTGCGTGGTGTCAACCGTCATAGTTTCCGTCCCGAAAGCGGTCGTACCCTAAGTAAAGCGAAGAATATTGAAGATGTACT 1163 2 873 .......................................................................................... 962 2 2652 ............ 2663 1 568 .................................................................................G........ 479 L L M K G M N M N S V R L S H Y P A D P E F L E A C D S L G Query_1 1164 TCTGATGAAGGGCATGAATATGAATTCTGTCCGTCTGAGCCACTATCCGGCGGACCCGGAATTTCTGGAAGCATGCGACTCTCTTGGACT 1253 2 963 .......................................................................................... 1052 2 498 .....T........... 514 2 2661 ........... 2671 1 478 .......................................................................................... 389 L Y V M D E L G G W H G K Y D T P T G V R L I E G M I E R D Query_1 1254 CTATGTTATGGATGAACTGGGTGGCTGGCATGGCAAGTACGACACCCCTACGGGAGTACGTCTGATTGAAGGCATGATAGAACGTGATGT 1343 2 1053 .......................................................................................... 1142 1 388 .......................................................................................... 299 V N H P S I I W W S N G N E K G W N I E L D G E F H K Y D L Query_1 1344 GAACCATCCGTCCATTATCTGGTGGAGCAATGGTAATGAAAAAGGCTGGAACATTGAACTGGACGGAGAATTCCATAAATACGATCTGCA 1433 2 1143 .......................................................................................... 1232 2 568 ........... 558 0 3 ........................................ 42 1 298 .......................................................................................... 209 Q K R P V I H P Q G N F S G F E T M H Y R S Y G E S Q N Y M Query_1 1434 GAAACGCCCGGTCATCCATCCGCAAGGTAACTTCTCCGGTTTCGAAACCATGCACTATCGTTCGTATGGAGAAAGCCAGAACTACATGCG 1523 2 1233 .......................................................................................... 1322 2 1281 ............ 1270 2 2323 ............ 2334 0 43 .......................................................................................... 132 0 91 ............ 80 1 208 .......................................................................................... 119 1 160 ............ 171 R L P E I F M P T A F L H G L Y D G G H G A G L Y D Y W E M Query_1 1524 CCTGCCGGAAATCTTTATGCCTACTGCTTTCCTGCATGGTTTGTACG-ACGGAGGTCATGGTGCCGGCCTGTATGATTACTGGGAAATGA 1612 2 1323 ..........................AA...................-.......................................... 1411 0 133 ...............................................-.......................................... 221 1 118 ...............................................A.......................................... 29 M R K H P R C I G G F L W V L A D E G V K R V D M D G F I D Query_1 1613 TGCGTAAACATCCGCGTTGTATCGGTGGTTTCCTGTGGGTATTGGCGGATGAAGGCGTGAAGCGCGTGGATATGGACGGGTTCATAGACA 1702 2 1412 .......................................................................................... 1501 0 222 .......................................................................................... 311 1 28 .........-....... 13 N Q G N F G A D G I V G P H H E K E G S Y Y T I K Q L W S P Query_1 1703 ATCAGGGAAATTTCGGAGCTGACGGAATTGTAGGCCCTCACCATGAAAAGGAAGGCAGCTATTACACTATCAAGCAGCTATGGAGCCCGG 1792 2 1502 .......................................................................................... 1591 0 312 .......................................................................................... 401 V Q V M N T A I D R N F D G K L S V E N R Y D Y L N L N T C Query_1 1793 TGCAGGTTATGAATACCGCTATCGACCGGAATTTCGACGGTAAACTCTCTGTGGAGAACCGTTATGATTATCTGAACCTGAACACCTGTC 1882 2 1592 .......................................................................................... 1681 0 402 .......................................................................................... 491 R F I W Q Q V K F P S V T D A S N T T T R I L K Q G E V Q G Query_1 1883 GTTTTATCTGGCAGCAAGTGAAGTTCCCGTCGGTAACGGATGCTTCCAATACAACTACACGGATTCTGAAACAAGGTGAAGTGCAAGGAA 1972 2 1682 .......................................................................................... 1771 2 234 ........... 244 0 492 ................................................N......................................... 581 S D V A A H G V G V V D I K T S I L P E A D A L F L T V I D Query_1 1973 GCGATGTAGCAGCCCATGGAGTGGGAGTGGTGGATATCAAGACTTCTATTCTTCCCGAAGCGGATGCTCTTTTCCTGACAGTTATAGATA 2062 2 1772 .......................................................................................... 1861 0 582 ........N..............N.................................................................. 671 K Y G Y E L W R W T F P V D K L N R E T E Q F S A S S G R Query_1 2063 AATATGGGTATGAACTTTGGCGCTGGACTT-TCCCCGTAGATAAA-CTGAATCG-GGAAACAGAACAGTTTT-CTGCATCATCCGGCCGT 2148 2 1862 ..............................-..............-........-.................-................. 1947 2 477 ........... 487 0 672 ..............................N......N.N.....C........TN................C....NN........... 761 V S Y T E T E K G I T V K A N G R T F V F S K K D G Q Query_1 2149 GTAT-CCTATAC--GGAAACAG-AAAAAGGTATTACGG-TAAAAGCAAACGGG--CGTACTTTT--GTCTTTTCAAAGAAAGACGGGCAG 2229 2 1948 ....-.......--........-...............-..............--.........--........................ 2028 0 762 ....C.......CG.A......A.....N.........N.....N.....N..TCNN.......TG.CT..................... 851 L K D V S V N N R K I S F A N G P R F I G A R R A D R Query_1 2230 CTGAAA-GATGTA-TCCGTCAATAACC-GTAAG-ATTAGTTTTGCTAA-CGGT-CCCCGTTT-TATCGGTGCACGTCGTGCAGACCGTTC 2312 2 2029 ......-......-.............-.....-..............-....-........-........................... 2111 0 852 N.N...N...N..T..NNC........NNN...A..N.T.........C.N..C........N...... 920 S L D Q F Y N H D D E K A K A K D R T Y S E F T D A A V F T Query_1 2313 CCTAGATCAGTTCTATAATCATGATGACGAAAAAGCCAAGGCAAAGGACCGTACTTACAGTGAATTTACCGATGCGGCAGTCTTCACGAA 2402 2 2112 .......................................................................................... 2201 0 835 ........... 825 K L D V K E E G G N L I L T A N Y K L G N L D K A Q W T I H Query_1 2403 ACTGGATGTGAAAGAAGAgggggggAATCTGATCCTCACCGCTAATTATAAACTGGGTAATTTAGATAAAGCTCAGTGGACAATTCATCC 2492 2 2202 .......................................................................................... 2291 P D G M A T L D Y T Y N F S G V V D L M G I C F D Y P E E Q Query_1 2493 GGACGGCATGGCTACTCTTGATTATACCTACAACTTCTCCGGTGTGGTAGACCTGATGGGTATTTGCTTTGATTACCCTGAAGAACAAGT 2582 2 2292 .......................................................................................... 2381 2 1261 ............ 1272 0 71 ............ 82 1 180 ............ 169 V L S K R W L G A G P Y R V W Q N R I H G T Q Y D I W E N D Query_1 2583 GCTCAGCAAGCGTTGGTTGGGAGCAGGTCCGTATCGTGTATGGCAGAATCGTATTCATGGCACGCAGTATGATATCTGGGAGAATGATTA 2672 2 2382 .......................................................................................... 2471 Y N D P I P G E T F T Y P E F K G Y F G S V S W M S I R T K Query_1 2673 TAACGATCCTATTCCGGGTGAGACATTCACCTATCCTGAATTCAAGGGATATTTTGGCAGTGTCTCTTGGATGAGTATTCGCACGAAAGA 2762 2 2472 .......................................................................................... 2561 E G T I S L T N E T P D S Y I G V Y Q P R D G R D R L L Y T Query_1 2763 GGGAACCATCAGCCTGACGAATGAAACACCTGATTCCTATATCGGAGTATATCAACCCCGTGATGGTCGTGACCGGTTACTGTATACACT 2852 2 2562 .......................................................................................... 2651 L P E S G I S V L N V I P P V R N K V N S T D L C G P S S Q Query_1 2853 TCCCGAAAGCGGAATTTCTGTTTTGAATGTAATTCCTCCGGTGCGTAATAAAGTAAATTCCACGGACTTGTGCGGTCCTTCTTCACAACC 2942 2 2652 .......................................................................................... 2741 2 912 ............ 923 2 1019 ........... 1029 1 529 ............ 518 1 422 ........... 412 P K W V D G S Q T G R L V I R F E Query_1 2943 AAAATGGGTGGATGGCTCGCAAACGGGACGCCTTGTTATCCGGTTTGAA 2991 2 2742 ................................................. 2790 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 17124030 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 5,444 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200018.108 (CMR200018.20-949.P(949).E469Q.pMCSG68)
QTLPQTERQYLSGHGCDDTVEWDFFCTDGRNSGRWTKIGVPSCWELQGFGTYQYGISFYGKAFPEGIAGEKGMYKYEFEVPEEFRGKQVSLVFEASMTDTEVKVNGRKAGSKHQGAFYCFSYNVTDLLKYGKKNQLEVTVSKESENASVNLAERRADYWNFGGIFRPVFLEVKPAVNLRHIAIDAQMDGSFRANCYTNISGDGMSIRAQILDGKGKKLADTTVPLKAGSDWTTLQLNVSAPALWTAETPNLYKAQFSLLDKGGKVLHHETETFGFRTIEVRESDGLYVNGVRINVRGVNRHSFRPESGRTLSKAKNIEDVLLMKGMNMNSVRLSHYPADPEFLEACDSLGLYVMDELGGWHGKYDTPTGVRLIEGMIERDVNHPSIIWWSNGNEKGWNIELDGEFHKYDLQKRPVIHPQGNFSGFETMHYRSYGESQNYMRLPEIFMPTEFLHGLYDGGHGAGLYDYWEMMRKHPRCIGGFLWVLADEGVKRVDMDGFIDNQGNFGADGIVGPHHEKEGSYYTIKQLWSPVQVMNTAIDRNFDGKLSVENRYDYLNLNTCRFIWQQVKFPSVTDASNTTTRILKQGEVQGSDVAAHGVGVVDIKTSILPEADALFLTVIDKYGYELWRWTFPVDKLNRETEQFSASSGRVSYTETEKGITVKANGRTFVFSKKDGQLKDVSVNNRKISFANGPRFIGARRADRSLDQFYNHDDEKAKAKDRTYSEFTDAAVFTKLDVKEEGGNLILTANYKLGNLDKAQWTIHPDGMATLDYTYNFSGVVDLMGICFDYPEEQVLSKRWLGAGPYRVWQNRIHGTQYDIWENDYNDPIPGETFTYPEFKGYFGSVSWMSIRTKEGTISLTNETPDSYIGVYQPRDGRDRLLYTLPESGISVLNVIPPVRNKVNSTDLCGPSSQPKWVDGSQTGRLVIRFE
CAGACACTTCCGCAGACAGAGCGGCAATACCTCTCCGGCCACGGATGCGACGACACAGTAGAATGGGACTTTTTCTGTACCGACGGACGTAACTCCGGTCGATGGACGAAAATAGGCGTCCCCTCTTGCTGGGAGTTGCAGGGTTTTGGTACCTATCAGTATGGAATTAGTTTTTATGGTAAAGCCTTTCCCGAAGGCATTGCCGGTGAGAAAGGAATGTATAAATATGAGTTTGAAGTTCCCGAGGAATTTCGTGGCAAGCAGGTCAGCCTTGTGTTCGAAGCATCCATGACCGATACGGAAGTTAAGGTTAACGGACGTAAGGCAGGATCGAAACACCAGGGAGCCTTCTATTGCTTTTCATATAATGTCACGGATTTACTGAAATATGGCAAGAAGAATCAGCTGGAAGTAACAGTTTCCAAGGAGAGTGAGAATGCCAGTGTGAATCTTGCCGAACGGCGCGCCGATTATTGGAACTTTGGCGGTATCTTCCGCCCGGTATTTCTGGAAGTAAAACCTGCCGTCAATCTCCGTCATATTGCTATTGATGCACAAATGGACGGATCATTCCGTGCCAATTGCTACACGAATATCTCCGGTGACGGAATGAGTATCCGTGCACAGATTTTGGACGGTAAAGGGAAGAAACTGGCAGATACCACCGTACCCCTAAAAGCCGGAAGCGACTGGACTACTTTACAATTGAACGTTTCTGCCCCTGCCTTATGGACGGCAGAAACTCCGAATCTTTATAAAGCTCAATTTTCACTGTTGGATAAAGGAGGTAAAGTCCTGCATCATGAGACCGAGACATTCGGTTTCCGTACTATCGAAGTTCGTGAAAGTGACGGATTGTACGTGAACGGGGTGCGTATCAACGTGCGTGGTGTCAACCGTCATAGTTTCCGTCCCGAAAGCGGTCGTACCCTAAGTAAAGCGAAGAATATTGAAGATGTACTTCTGATGAAGGGCATGAATATGAATTCTGTCCGTCTGAGCCACTATCCGGCGGACCCGGAATTTCTGGAAGCATGCGACTCTCTTGGACTCTATGTTATGGATGAACTGGGTGGCTGGCATGGCAAGTACGACACCCCTACGGGAGTACGTCTGATTGAAGGCATGATAGAACGTGATGTGAACCATCCGTCCATTATCTGGTGGAGCAATGGTAATGAAAAAGGCTGGAACATTGAACTGGACGGAGAATTCCATAAATACGATCTGCAGAAACGCCCGGTCATCCATCCGCAAGGTAACTTCTCCGGTTTCGAAACCATGCACTATCGTTCGTATGGAGAAAGCCAGAACTACATGCGCCTGCCGGAAATCTTTATGCCTACTGAATTCCTGCATGGTTTGTACGACGGAGGTCATGGTGCCGGCCTGTATGATTACTGGGAAATGATGCGTAAACATCCGCGTTGTATCGGTGGTTTCCTGTGGGTATTGGCGGATGAAGGCGTGAAGCGCGTGGATATGGACGGGTTCATAGACAATCAGGGAAATTTCGGAGCTGACGGAATTGTAGGCCCTCACCATGAAAAGGAAGGCAGCTATTACACTATCAAGCAGCTATGGAGCCCGGTGCAGGTTATGAATACCGCTATCGACCGGAATTTCGACGGTAAACTCTCTGTGGAGAACCGTTATGATTATCTGAACCTGAACACCTGTCGTTTTATCTGGCAGCAAGTGAAGTTCCCGTCGGTAACGGATGCTTCCAATACAACTACACGGATTCTGAAACAAGGTGAAGTGCAAGGAAGCGATGTAGCAGCCCATGGAGTGGGAGTGGTGGATATCAAGACTTCTATTCTTCCCGAAGCGGATGCTCTTTTCCTGACAGTTATAGATAAATATGGGTATGAACTTTGGCGCTGGACTTTCCCCGTAGATAAACTGAATCGGGAAACAGAACAGTTTTCTGCATCATCCGGCCGTGTATCCTATACGGAAACAGAAAAAGGTATTACGGTAAAAGCAAACGGGCGTACTTTTGTCTTTTCAAAGAAAGACGGGCAGCTGAAAGATGTATCCGTCAATAACCGTAAGATTAGTTTTGCTAACGGTCCCCGTTTTATCGGTGCACGTCGTGCAGACCGTTCCCTAGATCAGTTCTATAATCATGATGACGAAAAAGCCAAGGCAAAGGACCGTACTTACAGTGAATTTACCGATGCGGCAGTCTTCACGAAACTGGATGTGAAAGAAGAGGGGGGGAATCTGATCCTCACCGCTAATTATAAACTGGGTAATTTAGATAAAGCTCAGTGGACAATTCATCCGGACGGCATGGCTACTCTTGATTATACCTACAACTTCTCCGGTGTGGTAGACCTGATGGGTATTTGCTTTGATTACCCTGAAGAACAAGTGCTCAGCAAGCGTTGGTTGGGAGCAGGTCCGTATCGTGTATGGCAGAATCGTATTCATGGCACGCAGTATGATATCTGGGAGAATGATTATAACGATCCTATTCCGGGTGAGACATTCACCTATCCTGAATTCAAGGGATATTTTGGCAGTGTCTCTTGGATGAGTATTCGCACGAAAGAGGGAACCATCAGCCTGACGAATGAAACACCTGATTCCTATATCGGAGTATATCAACCCCGTGATGGTCGTGACCGGTTACTGTATACACTTCCCGAAAGCGGAATTTCTGTTTTGAATGTAATTCCTCCGGTGCGTAATAAAGTAAATTCCACGGACTTGTGCGGTCCTTCTTCACAACCAAAATGGGTGGATGGCTCGCAAACGGGACGCCTTGTTATCCGGTTTGAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,220 total letters Query= CMR200018.108 Length=3195 Score E Sequences producing significant alignments: (Bits) Value wt 5023 0.0 10A_A7_CMR200018.108_A07.seq 1104 0.0 Q T L P Q T E R Q Y L S G H G C D D T V E W D F F C T D G R Query_1 202 CAGACACTTCCGCAGACAGAGCGGCAATACCTCTCCGGCCACGGATGCGACGACACAGTAGAATGGGACTTTTTCTGTACCGACGGACGT 291 2 1 .......................................................................................... 90 N S G R W T K I G V P S C W E L Q G F G T Y Q Y G I S F Y G Query_1 292 AACTCCGGTCGATGGACGAAAATAGGCGTCCCCTCTTGCTGGGAGTTGCAGGGTTTTGGTACCTATCAGTATGGAATTAGTTTTTATGGT 381 2 91 .......................................................................................... 180 K A F P E G I A G E K G M Y K Y E F E V P E E F R G K Q V S Query_1 382 AAAGCCTTTCCCGAAGGCATTGCCGGTGAGAAAGGAATGTATAAATATGAGTTTGAAGTTCCCGAGGAATTTCGTGGCAAGCAGGTCAGC 471 2 181 .......................................................................................... 270 L V F E A S M T D T E V K V N G R K A G S K H Q G A F Y C F Query_1 472 CTTGTGTTCGAAGCATCCATGACCGATACGGAAGTTAAGGTTAACGGACGTAAGGCAGGATCGAAACACCAGGGAGCCTTCTATTGCTTT 561 2 271 .......................................................................................... 360 S Y N V T D L L K Y G K K N Q L E V T V S K E S E N A S V N Query_1 562 TCATATAATGTCACGGATTTACTGAAATATGGCAAGAAGAATCAGCTGGAAGTAACAGTTTCCAAGGAGAGTGAGAATGCCAGTGTGAAT 651 2 361 .......................................................................................... 450 L A E R R A D Y W N F G G I F R P V F L E V K P A V N L R H Query_1 652 CTTGCCGAACGGCGCGCCGATTATTGGAACTTTGGCGGTATCTTCCGCCCGGTATTTCTGGAAGTAAAACCTGCCGTCAATCTCCGTCAT 741 2 451 .......................................................................................... 540 2 1017 .....A........... 1033 2 1873 ........... 1883 I A I D A Q M D G S F R A N C Y T N I S G D G M S I R A Q I Query_1 742 ATTGCTATTGATGCACAAATGGACGGATCATTCCGTGCCAATTGCTACACGAATATCTCCGGTGACGGAATGAGTATCCGTGCACAGATT 831 2 541 .......................................................................................... 630 L D G K G K K L A D T T V P L K A G S D W T T L Q L N V S A Query_1 832 TTGGACGGTAAAGGGAAGAAACTGGCAGATACCACCGTACCCCTAAAAGCCGGAAGCGACTGGACTACTTTACAATTGAACGTTTCTGCC 921 2 631 .......................................................................................... 720 P A L W T A E T P N L Y K A Q F S L L D K G G K V L H H E T Query_1 922 CCTGCCTTATGGACGGCAGAAACTCCGAATCTTTATAAAGCTCAATTTTCACTGTTGGATAAAGGAGGTAAAGTCCTGCATCATGAGACC 1011 2 721 .......................................................................................... 810 E T F G F R T I E V R E S D G L Y V N G V R I N V R G V N R Query_1 1012 GAGACATTCGGTTTCCGTACTATCGAAGTTCGTGAAAGTGACGGATTGTACGTGAACGGGGTGCGTATCAACGTGCGTGGTGTCAACCGT 1101 2 811 .......................................................................................... 900 H S F R P E S G R T L S K A K N I E D V L L M K G M N M N S Query_1 1102 CATAGTTTCCGTCCCGAAAGCGGTCGTACCCTAAGTAAAGCGAAGAATATTGAAGATGTACTTCTGATGAAGGGCATGAATATGAATTCT 1191 2 901 .......................................................................................... 990 2 2652 ............ 2663 V R L S H Y P A D P E F L E A C D S L G L Y V M D E L G G W Query_1 1192 GTCCGTCTGAGCCACTATCCGGCGGACCCGGAATTTCTGGAAGCATGCGACTCTCTTGGACTCTATGTTATGGATGAACTGGGTGGCTGG 1281 2 991 .......................................................................................... 1080 2 498 .....T........... 514 2 2661 ........... 2671 H G K Y D T P T G V R L I E G M I E R D V N H P S I I W W S Query_1 1282 CATGGCAAGTACGACACCCCTACGGGAGTACGTCTGATTGAAGGCATGATAGAACGTGATGTGAACCATCCGTCCATTATCTGGTGGAGC 1371 2 1081 .......................................................................................... 1170 2 568 ........... 558 N G N E K G W N I E L D G E F H K Y D L Q K R P V I Query_1 1372 AATGGTAATGAAAAAGGC-TGG-AACATTGAAC-TGGACGGAG-AATTCCAT--AAATACGAT-CTGCAGAAA--CGCCC-GGTCATCC- 1450 2 1171 ..................-...-..........-.........-........--.........-.........--.....-........- 1249 1 1050 ....N...N......NN...N...N..N...C.....N.N.N....N.C.TA....NN...T.G.......ACN....C....N.N.N 963 H P Q G N F S G F E T M H Y R S Y G E S Q N Y M R L P Query_1 1451 ATCCGC-AAGGTAACTTCTCCGG--TTTCGA-AACCATGCACTATCG--TTCGTATGGAGAAAGCCAGAACTACATGCGCC-TG-CCGGA 1532 2 1250 ......-................--......-...............--................................-..-..... 1331 2 1281 ..--......-.... 1270 2 2323 ...........--. 2334 1 962 ..N.C.N.......N..A.T.N.NA....N.G.....N..NN.T.NNGT..N...N...N....N....TG.......C..C..N....N 873 E I F M P T Q F L H G L Y D G G H G A G L Y D Y W E Query_1 1533 AAT--CTTTATGCCTACTC--AGTTCCT-GCATGGTTT-GTACGACGG-AGGTCATGG-TGCCGG--CCTGTATGATTA-CTGGG--AAA 1609 2 1332 ...--.............G--.A.....-.........-.........-.........-......--............-.....--... 1408 1 872 ...TCN..N.G..G...NNGA.T.N..NA..C.NANN.AN..NN....G..N......G.N..N.GC............N.....GA... 783 M M R K H P R C I G G F L W V L A D E G V K R V D M D G Query_1 1610 TGATGCGTAAACATCC-GCGTTGTATC-GGTGGTTTCCTGT-GGGTATTGGCGGATGAA-GGCGTGAA-GCGCGTGGATATGGACGGGTT 1694 2 1409 ................-..........-.............-.................-........-..................... 1493 1 782 ................N.....NN...C.............T.N...............N........C.N................N.. 693 F I D N Q G N F G A D G I V G P H H E K E G S Y Y T I K Q L Query_1 1695 CATAGACAATCA-GGGAAATTTCGGAGCTGACGGAA-TTGTAGGCCCTCACCATGAAAAGGAAGGCAGCTATTACACTATCAAGCAGCTA 1782 2 1494 ............-.......................-..................................................... 1581 1 692 .....N......T.......................A..................................................... 603 W S P V Q V M N T A I D R N F D G K L S V E N R Y D Y L N L Query_1 1783 TGGAGCCCGGTGCAGGTTATGAATACCGCTATCGACCGGAATTTCGACGGTAAACTCTCTGTGGAGAACCGTTATGATTATCTGAACCTG 1872 2 1582 .......................................................................................... 1671 1 602 .......................................................................................... 513 N T C R F I W Q Q V K F P S V T D A S N T T T R I L K Q G Query_1 1873 AACACCTGTCGTTTTATCTGGCAGCAAGTGAAGTTCCCGTCGGTAACGGATGCTTCCAATACAA-CTACACGGATTCTGAAACAAGGTGA 1961 2 1672 ................................................................-......................... 1760 2 234 ........... 244 1 512 ......................................N.........................T......................... 423 E V Q G S D V A A H G V G V V D I K T S I L P E A D A L F L Query_1 1962 AGTGCAAGGAAGCGATGTAGCAGCCCATGGAGTGGGAGTGGTGGATATCAAGACTTCTATTCTTCCCGAAGCGGATGCTCTTTTCCTGAC 2051 2 1761 .......................................................................................... 1850 1 422 .......................................................................................... 333 T V I D K Y G Y E L W R W T F P V D K L N R E T E Q F S A S Query_1 2052 AGTTATAGATAAATATGGGTATGAACTTTGGCGCTGGACTTTCCCCGTAGATAAACTGAATCGGGAAACAGAACAGTTTTCTGCATCATC 2141 2 1851 .......................................................................................... 1940 2 477 ........... 487 1 332 .......................................................................................... 243 S G R V S Y T E T E K G I T V K A N G R T F V F S K K D G Q Query_1 2142 CGGCCGTGTATCCTATACGGAAACAGAAAAAGGTATTACGGTAAAAGCAAACGGGCGTACTTTTGTCTTTTCAAAGAAAGACGGGCAGCT 2231 2 1941 .......................................................................................... 2030 1 242 ..............................................................................------------ 165 L K D V S V N N R K I S F A N G P R Query_1 2232 GAAAGATGTATCCGTCAATAACCGTA--------------------------------------AGATTAGTTTTGCTAACGGTCCCCGT 2283 2 2031 ..........................--------------------------------------.......................... 2082 1 164 --------------------------AGACGGGCAGCTGAAAGATGTATCCGTCAATAACCGTA.......................... 101 F I G A R R A D R S L D Q F Y N H D D E K A K A K D R T Y S Query_1 2284 TTTATCGGTGCACGTCGTGCAGACCGTTCCCTAGATCAGTTCTATAATCATGATGACGAAAAAGCCAAGGCAAAGGACCGTACTTACAGT 2373 2 2083 .......................................................................................... 2172 1 100 ............AC.......................................................N..........-..-.....- 14 E F T D A A V F T K L D V K E E G G N L I L T A N Y K L G N Query_1 2374 GAATTTACCGATGCGGCAGTCTTCACGAAACTGGATGTGAAAGAAGAgggggggAATCTGATCCTCACCGCTAATTATAAACTGGGTAAT 2463 2 2173 .......................................................................................... 2262 1 13 ..... 9 L D K A Q W T I H P D G M A T L D Y T Y N F S G V V D L M G Query_1 2464 TTAGATAAAGCTCAGTGGACAATTCATCCGGACGGCATGGCTACTCTTGATTATACCTACAACTTCTCCGGTGTGGTAGACCTGATGGGT 2553 2 2263 .......................................................................................... 2352 2 1261 ............ 1272 I C F D Y P E E Q V L S K R W L G A G P Y R V W Q N R I H G Query_1 2554 ATTTGCTTTGATTACCCTGAAGAACAAGTGCTCAGCAAGCGTTGGTTGGGAGCAGGTCCGTATCGTGTATGGCAGAATCGTATTCATGGC 2643 2 2353 .......................................................................................... 2442 T Q Y D I W E N D Y N D P I P G E T F T Y P E F K G Y F G S Query_1 2644 ACGCAGTATGATATCTGGGAGAATGATTATAACGATCCTATTCCGGGTGAGACATTCACCTATCCTGAATTCAAGGGATATTTTGGCAGT 2733 2 2443 .......................................................................................... 2532 V S W M S I R T K E G T I S L T N E T P D S Y I G V Y Q P R Query_1 2734 GTCTCTTGGATGAGTATTCGCACGAAAGAGGGAACCATCAGCCTGACGAATGAAACACCTGATTCCTATATCGGAGTATATCAACCCCGT 2823 2 2533 .......................................................................................... 2622 D G R D R L L Y T L P E S G I S V L N V I P P V R N K V N S Query_1 2824 GATGGTCGTGACCGGTTACTGTATACACTTCCCGAAAGCGGAATTTCTGTTTTGAATGTAATTCCTCCGGTGCGTAATAAAGTAAATTCC 2913 2 2623 .......................................................................................... 2712 2 912 ............ 923 2 1019 ........... 1029 T D L C G P S S Q P K W V D G S Q T G R L V I R F E Query_1 2914 ACGGACTTGTGCGGTCCTTCTTCACAACCAAAATGGGTGGATGGCTCGCAAACGGGACGCCTTGTTATCCGGTTTGAA 2991 2 2713 .............................................................................. 2790 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 13249082 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 4,220 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200018.109 (CMR200018.20-949.P(949).R718T.pMCSG68)
QTLPQTERQYLSGHGCDDTVEWDFFCTDGRNSGRWTKIGVPSCWELQGFGTYQYGISFYGKAFPEGIAGEKGMYKYEFEVPEEFRGKQVSLVFEASMTDTEVKVNGRKAGSKHQGAFYCFSYNVTDLLKYGKKNQLEVTVSKESENASVNLAERRADYWNFGGIFRPVFLEVKPAVNLRHIAIDAQMDGSFRANCYTNISGDGMSIRAQILDGKGKKLADTTVPLKAGSDWTTLQLNVSAPALWTAETPNLYKAQFSLLDKGGKVLHHETETFGFRTIEVRESDGLYVNGVRINVRGVNRHSFRPESGRTLSKAKNIEDVLLMKGMNMNSVRLSHYPADPEFLEACDSLGLYVMDELGGWHGKYDTPTGVRLIEGMIERDVNHPSIIWWSNGNEKGWNIELDGEFHKYDLQKRPVIHPQGNFSGFETMHYRSYGESQNYMRLPEIFMPTEFLHGLYDGGHGAGLYDYWEMMRKHPRCIGGFLWVLADEGVKRVDMDGFIDNQGNFGADGIVGPHHEKEGSYYTIKQLWSPVQVMNTAIDRNFDGKLSVENRYDYLNLNTCRFIWQQVKFPSVTDASNTTTRILKQGEVQGSDVAAHGVGVVDIKTSILPEADALFLTVIDKYGYELWRWTFPVDKLNRETEQFSASSGRVSYTETEKGITVKANGRTFVFSKKDGQLKDVSVNNRKISFANGPRFIGARRADRSLDQFYNHDDEKAKAKDRTYSEFTDAAVFTKLDVKEEGGNLILTANYKLGNLDKAQWTIHPDGMATLDYTYNFSGVVDLMGICFDYPEEQVLSKRWLGAGPYRVWQNRIHGTQYDIWENDYNDPIPGETFTYPEFKGYFGSVSWMSIRTKEGTISLTNETPDSYIGVYQPRDGRDRLLYTLPESGISVLNVIPPVRNKVNSTDLCGPSSQPKWVDGSQTGRLVIRFE
CAGACACTTCCGCAGACAGAGCGGCAATACCTCTCCGGCCACGGATGCGACGACACAGTAGAATGGGACTTTTTCTGTACCGACGGACGTAACTCCGGTCGATGGACGAAAATAGGCGTCCCCTCTTGCTGGGAGTTGCAGGGTTTTGGTACCTATCAGTATGGAATTAGTTTTTATGGTAAAGCCTTTCCCGAAGGCATTGCCGGTGAGAAAGGAATGTATAAATATGAGTTTGAAGTTCCCGAGGAATTTCGTGGCAAGCAGGTCAGCCTTGTGTTCGAAGCATCCATGACCGATACGGAAGTTAAGGTTAACGGACGTAAGGCAGGATCGAAACACCAGGGAGCCTTCTATTGCTTTTCATATAATGTCACGGATTTACTGAAATATGGCAAGAAGAATCAGCTGGAAGTAACAGTTTCCAAGGAGAGTGAGAATGCCAGTGTGAATCTTGCCGAACGGCGCGCCGATTATTGGAACTTTGGCGGTATCTTCCGCCCGGTATTTCTGGAAGTAAAACCTGCCGTCAATCTCCGTCATATTGCTATTGATGCACAAATGGACGGATCATTCCGTGCCAATTGCTACACGAATATCTCCGGTGACGGAATGAGTATCCGTGCACAGATTTTGGACGGTAAAGGGAAGAAACTGGCAGATACCACCGTACCCCTAAAAGCCGGAAGCGACTGGACTACTTTACAATTGAACGTTTCTGCCCCTGCCTTATGGACGGCAGAAACTCCGAATCTTTATAAAGCTCAATTTTCACTGTTGGATAAAGGAGGTAAAGTCCTGCATCATGAGACCGAGACATTCGGTTTCCGTACTATCGAAGTTCGTGAAAGTGACGGATTGTACGTGAACGGGGTGCGTATCAACGTGCGTGGTGTCAACCGTCATAGTTTCCGTCCCGAAAGCGGTCGTACCCTAAGTAAAGCGAAGAATATTGAAGATGTACTTCTGATGAAGGGCATGAATATGAATTCTGTCCGTCTGAGCCACTATCCGGCGGACCCGGAATTTCTGGAAGCATGCGACTCTCTTGGACTCTATGTTATGGATGAACTGGGTGGCTGGCATGGCAAGTACGACACCCCTACGGGAGTACGTCTGATTGAAGGCATGATAGAACGTGATGTGAACCATCCGTCCATTATCTGGTGGAGCAATGGTAATGAAAAAGGCTGGAACATTGAACTGGACGGAGAATTCCATAAATACGATCTGCAGAAACGCCCGGTCATCCATCCGCAAGGTAACTTCTCCGGTTTCGAAACCATGCACTATCGTTCGTATGGAGAAAGCCAGAACTACATGCGCCTGCCGGAAATCTTTATGCCTACTGAATTCCTGCATGGTTTGTACGACGGAGGTCATGGTGCCGGCCTGTATGATTACTGGGAAATGATGCGTAAACATCCGCGTTGTATCGGTGGTTTCCTGTGGGTATTGGCGGATGAAGGCGTGAAGCGCGTGGATATGGACGGGTTCATAGACAATCAGGGAAATTTCGGAGCTGACGGAATTGTAGGCCCTCACCATGAAAAGGAAGGCAGCTATTACACTATCAAGCAGCTATGGAGCCCGGTGCAGGTTATGAATACCGCTATCGACCGGAATTTCGACGGTAAACTCTCTGTGGAGAACCGTTATGATTATCTGAACCTGAACACCTGTCGTTTTATCTGGCAGCAAGTGAAGTTCCCGTCGGTAACGGATGCTTCCAATACAACTACACGGATTCTGAAACAAGGTGAAGTGCAAGGAAGCGATGTAGCAGCCCATGGAGTGGGAGTGGTGGATATCAAGACTTCTATTCTTCCCGAAGCGGATGCTCTTTTCCTGACAGTTATAGATAAATATGGGTATGAACTTTGGCGCTGGACTTTCCCCGTAGATAAACTGAATCGGGAAACAGAACAGTTTTCTGCATCATCCGGCCGTGTATCCTATACGGAAACAGAAAAAGGTATTACGGTAAAAGCAAACGGGCGTACTTTTGTCTTTTCAAAGAAAGACGGGCAGCTGAAAGATGTATCCGTCAATAACCGTAAGATTAGTTTTGCTAACGGTCCCCGTTTTATCGGTGCACGTCGTGCAGACCGTTCCCTAGATCAGTTCTATAATCATGATGACGAAAAAGCCAAGGCAAAGGACCGTACTTACAGTGAATTTACCGATGCGGCAGTCTTCACGAAACTGGATGTGAAAGAAGAGGGGGGGAATCTGATCCTCACCGCTAATTATAAACTGGGTAATTTAGATAAAGCTCAGTGGACAATTCATCCGGACGGCATGGCTACTCTTGATTATACCTACAACTTCTCCGGTGTGGTAGACCTGATGGGTATTTGCTTTGATTACCCTGAAGAACAAGTGCTCAGCAAGCGTTGGTTGGGAGCAGGTCCGTATCGTGTATGGCAGAATCGTATTCATGGCACGCAGTATGATATCTGGGAGAATGATTATAACGATCCTATTCCGGGTGAGACATTCACCTATCCTGAATTCAAGGGATATTTTGGCAGTGTCTCTTGGATGAGTATTCGCACGAAAGAGGGAACCATCAGCCTGACGAATGAAACACCTGATTCCTATATCGGAGTATATCAACCCCGTGATGGTCGTGACCGGTTACTGTATACACTTCCCGAAAGCGGAATTTCTGTTTTGAATGTAATTCCTCCGGTGCGTAATAAAGTAAATTCCACGGACTTGTGCGGTCCTTCTTCACAACCAAAATGGGTGGATGGCTCGCAAACGGGACGCCTTGTTATCCGGTTTGAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,957 total letters Query= CMR200018.109 Length=3195 Score E Sequences producing significant alignments: (Bits) Value wt 5023 0.0 10B_B7_CMR200018.109_B07.seq 1862 0.0 Q T L P Q T E R Q Y L S G H G C D D T V E W D F F C T D G R Query_1 202 CAGACACTTCCGCAGACAGAGCGGCAATACCTCTCCGGCCACGGATGCGACGACACAGTAGAATGGGACTTTTTCTGTACCGACGGACGT 291 2 1 .......................................................................................... 90 N S G R W T K I G V P S C W E L Q G F G T Y Q Y G I S F Y G Query_1 292 AACTCCGGTCGATGGACGAAAATAGGCGTCCCCTCTTGCTGGGAGTTGCAGGGTTTTGGTACCTATCAGTATGGAATTAGTTTTTATGGT 381 2 91 .......................................................................................... 180 K A F P E G I A G E K G M Y K Y E F E V P E E F R G K Q V S Query_1 382 AAAGCCTTTCCCGAAGGCATTGCCGGTGAGAAAGGAATGTATAAATATGAGTTTGAAGTTCCCGAGGAATTTCGTGGCAAGCAGGTCAGC 471 2 181 .......................................................................................... 270 L V F E A S M T D T E V K V N G R K A G S K H Q G A F Y C Query_1 472 CTTGTGTTCGAAGCATCCATGACCGATACGGAAGTTAAGGTTAACGGACG--TAAGGCAGGATCGAAACACCAGGGAGCCTTCTATTGCT 559 2 271 ..................................................--...................................... 358 1 1151 .....AG........N.N.NANNT..........N....N...........T....N....C 1090 F S Y N V T D L L K Y G K K N Q L E V T V S K E S E N A Query_1 560 TTT--CATATAATGTCACGGATTTACT-GAAATATGGCAAGAAGAATCAGCTGGAA-GTAACAGTTTCCAAGGAG-AGTGAGAATGCCAG 644 2 359 ...--......................-............................-..................-.............. 443 1 1089 ...TA.N......NCNN..........N................N...........N.........-..T.....G....N......... 1001 S V N L A E R R A D Y W N F G G I F R P V F L E V K P A V N Query_1 645 TGTGAATCTTGCCGAACGGCGCGCCGATTATTGGAACTTTGGCGGTATCTTCCGCCCGGTATTTCTGGAAGTAAAACCTGCCGTCAATCT 734 2 444 .......................................................................................... 533 2 1017 .....A........... 1033 2 1873 ........... 1883 1 1000 ............................A--.........T..........................N...................... 913 1 429 .....A........... 413 L R H I A I D A Q M D G S F R A N C Y T N I S G D G M S I R Query_1 735 CCGTCATATTGCTATTGATGCACAAATGGACGGATCATTCCGTGCCAATTGCTACACGAATATCTCCGGTGACGGAATGAGTATCCGTGC 824 2 534 .......................................................................................... 623 1 912 ...............-.......................................................................... 824 1 288 ........... 298 A Q I L D G K G K K L A D T T V P L K A G S D W T T L Q L N Query_1 825 ACAGATTTTGGACGGTAAA-GGGAAGAAACTGGCAGATACCACCGTACCCCTAAAAGCCGGAAGCGACTGGACTACTTTACAATTGAACG 913 2 624 ...................-...................................................................... 712 1 823 ...........N.......A...................................................................... 734 V S A P A L W T A E T P N L Y K A Q F S L L D K G G K V L H Query_1 914 TTTCTGCCCCTGCCTTATGGACGGCAGAAACTCCGAATCTTTATAAAGCTCAATTTTCACTGTTGGATAAAGGAGGTAAAGTCCTGCATC 1003 2 713 .......................................................................................... 802 1 733 .......................................................................................... 644 H E T E T F G F R T I E V R E S D G L Y V N G V R I N V R G Query_1 1004 ATGAGACCGAGACATTCGGTTTCCGTACTATCGAAGTTCGTGAAAGTGACGGATTGTACGTGAACGGGGTGCGTATCAACGTGCGTGGTG 1093 2 803 .......................................................................................... 892 1 643 .......................................................................................... 554 V N R H S F R P E S G R T L S K A K N I E D V L L M K G M N Query_1 1094 TCAACCGTCATAGTTTCCGTCCCGAAAGCGGTCGTACCCTAAGTAAAGCGAAGAATATTGAAGATGTACTTCTGATGAAGGGCATGAATA 1183 2 893 .......................................................................................... 982 2 2652 ............ 2663 1 553 .............................................................G............................ 464 M N S V R L S H Y P A D P E F L E A C D S L G L Y V M D E L Query_1 1184 TGAATTCTGTCCGTCTGAGCCACTATCCGGCGGACCCGGAATTTCTGGAAGCATGCGACTCTCTTGGACTCTATGTTATGGATGAACTGG 1273 2 983 .......................................................................................... 1072 2 498 .....T........... 514 2 2661 ........... 2671 1 463 .......................................................................................... 374 G G W H G K Y D T P T G V R L I E G M I E R D V N H P S I I Query_1 1274 GTGGCTGGCATGGCAAGTACGACACCCCTACGGGAGTACGTCTGATTGAAGGCATGATAGAACGTGATGTGAACCATCCGTCCATTATCT 1363 2 1073 .......................................................................................... 1162 2 568 ........... 558 1 373 .......................................................................................... 284 1 879 ........... 889 W W S N G N E K G W N I E L D G E F H K Y D L Q K R P V I H Query_1 1364 GGTGGAGCAATGGTAATGAAAAAGGCTGGAACATTGAACTGGACGGAGAATTCCATAAATACGATCTGCAGAAACGCCCGGTCATCCATC 1453 2 1163 .......................................................................................... 1252 1 283 .................C.G...................................................................... 194 P Q G N F S G F E T M H Y R S Y G E S Q N Y M R L P E I F M Query_1 1454 CGCAAGGTAACTTCTCCGGTTTCGAAACCATGCACTATCGTTCGTATGGAGAAAGCCAGAACTACATGCGCCTGCCGGAAATCTTTATGC 1543 2 1253 .......................................................................................... 1342 2 1281 ............ 1270 2 2323 ............ 2334 1 193 .......................................................................................... 104 1 165 ............ 176 P T E F L H G L Y D G G H G A G L Y D Y W E M M R K H P R C Query_1 1544 CTACTGAATTCCTGCATGGTTTGTACGACGGAGGTCATGGTGCCGGCCTGTATGATTACTGGGAAATGATGCGTAAACATCCGCGTTGTA 1633 2 1343 .......................................................................................... 1432 1 103 ....................................................................................... 17 I G G F L W V L A D E G V K R V D M D G F I D N Q G N F G A Query_1 1634 TCGGTGGTTTCCTGTGGGTATTGGCGGATGAAGGCGTGAAGCGCGTGGATATGGACGGGTTCATAGACAATCAGGGAAATTTCGGAGCTG 1723 2 1433 .......................................................................................... 1522 D G I V G P H H E K E G S Y Y T I K Q L W S P V Q V M N T A Query_1 1724 ACGGAATTGTAGGCCCTCACCATGAAAAGGAAGGCAGCTATTACACTATCAAGCAGCTATGGAGCCCGGTGCAGGTTATGAATACCGCTA 1813 2 1523 .......................................................................................... 1612 I D R N F D G K L S V E N R Y D Y L N L N T C R F I W Q Q V Query_1 1814 TCGACCGGAATTTCGACGGTAAACTCTCTGTGGAGAACCGTTATGATTATCTGAACCTGAACACCTGTCGTTTTATCTGGCAGCAAGTGA 1903 2 1613 .......................................................................................... 1702 2 234 ... 236 K F P S V T D A S N T T T R I L K Q G E V Q G S D V A A H G Query_1 1904 AGTTCCCGTCGGTAACGGATGCTTCCAATACAACTACACGGATTCTGAAACAAGGTGAAGTGCAAGGAAGCGATGTAGCAGCCCATGGAG 1993 2 1703 .......................................................................................... 1792 2 237 ........ 244 V G V V D I K T S I L P E A D A L F L T V I D K Y G Y E L W Query_1 1994 TGGGAGTGGTGGATATCAAGACTTCTATTCTTCCCGAAGCGGATGCTCTTTTCCTGACAGTTATAGATAAATATGGGTATGAACTTTGGC 2083 2 1793 .......................................................................................... 1882 2 477 .......... 486 R W T F P V D K L N R E T E Q F S A S S G R V S Y T E T E K Query_1 2084 GCTGGACTTTCCCCGTAGATAAACTGAATCGGGAAACAGAACAGTTTTCTGCATCATCCGGCCGTGTATCCTATACGGAAACAGAAAAAG 2173 2 1883 .......................................................................................... 1972 2 487 . 487 G I T V K A N G R T F V F S K K D G Q L K D V S V N N R K I Query_1 2174 GTATTACGGTAAAAGCAAACGGGCGTACTTTTGTCTTTTCAAAGAAAGACGGGCAGCTGAAAGATGTATCCGTCAATAACCGTAAGATTA 2263 2 1973 .......................................................................................... 2062 S F A N G P R F I G A T R A D R S L D Q F Y N H D D E K A K Query_1 2264 GTTTTGCTAACGGTCCCCGTTTTATCGGTGCAACTCGTGCAGACCGTTCCCTAGATCAGTTCTATAATCATGATGACGAAAAAGCCAAGG 2353 2 2063 ................................CG........................................................ 2152 A K D R T Y S E F T D A A V F T K L D V K E E G G N L I L T Query_1 2354 CAAAGGACCGTACTTACAGTGAATTTACCGATGCGGCAGTCTTCACGAAACTGGATGTGAAAGAAGAgggggggAATCTGATCCTCACCG 2443 2 2153 .......................................................................................... 2242 A N Y K L G N L D K A Q W T I H P D G M A T L D Y T Y N F S Query_1 2444 CTAATTATAAACTGGGTAATTTAGATAAAGCTCAGTGGACAATTCATCCGGACGGCATGGCTACTCTTGATTATACCTACAACTTCTCCG 2533 2 2243 .......................................................................................... 2332 2 1261 .......... 1270 1 185 .......... 176 G V V D L M G I C F D Y P E E Q V L S K R W L G A G P Y R V Query_1 2534 GTGTGGTAGACCTGATGGGTATTTGCTTTGATTACCCTGAAGAACAAGTGCTCAGCAAGCGTTGGTTGGGAGCAGGTCCGTATCGTGTAT 2623 2 2333 .......................................................................................... 2422 2 1271 .. 1272 1 175 .. 174 W Q N R I H G T Q Y D I W E N D Y N D P I P G E T F T Y P E Query_1 2624 GGCAGAATCGTATTCATGGCACGCAGTATGATATCTGGGAGAATGATTATAACGATCCTATTCCGGGTGAGACATTCACCTATCCTGAAT 2713 2 2423 .......................................................................................... 2512 F K G Y F G S V S W M S I R T K E G T I S L T N E T P D S Y Query_1 2714 TCAAGGGATATTTTGGCAGTGTCTCTTGGATGAGTATTCGCACGAAAGAGGGAACCATCAGCCTGACGAATGAAACACCTGATTCCTATA 2803 2 2513 .......................................................................................... 2602 I G V Y Q P R D G R D R L L Y T L P E S G I S V L N V I P P Query_1 2804 TCGGAGTATATCAACCCCGTGATGGTCGTGACCGGTTACTGTATACACTTCCCGAAAGCGGAATTTCTGTTTTGAATGTAATTCCTCCGG 2893 2 2603 .......................................................................................... 2692 2 912 ............ 923 2 1019 ........... 1029 1 534 ............ 523 1 427 ........... 417 V R N K V N S T D L C G P S S Q P K W V D G S Q T G R L V I Query_1 2894 TGCGTAATAAAGTAAATTCCACGGACTTGTGCGGTCCTTCTTCACAACCAAAATGGGTGGATGGCTCGCAAACGGGACGCCTTGTTATCC 2983 2 2693 .......................................................................................... 2782 R F E Query_1 2984 GGTTTGAA 2991 2 2783 ........ 2790 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12413268 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 3,957 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200018.111 (CMR200018.20-949.P(949).E413Q.pMCSG68)
QTLPQTERQYLSGHGCDDTVEWDFFCTDGRNSGRWTKIGVPSCWELQGFGTYQYGISFYGKAFPEGIAGEKGMYKYEFEVPEEFRGKQVSLVFEASMTDTEVKVNGRKAGSKHQGAFYCFSYNVTDLLKYGKKNQLEVTVSKESENASVNLAERRADYWNFGGIFRPVFLEVKPAVNLRHIAIDAQMDGSFRANCYTNISGDGMSIRAQILDGKGKKLADTTVPLKAGSDWTTLQLNVSAPALWTAETPNLYKAQFSLLDKGGKVLHHETETFGFRTIEVRESDGLYVNGVRINVRGVNRHSFRPESGRTLSKAKNIEDVLLMKGMNMNSVRLSHYPADPEFLEACDSLGLYVMDELGGWHGKYDTPTGVRLIEGMIERDVNHPSIIWWSNGNEKGWNIELDGEFHKYDLQKRPVIHPQGNFSGFETMHYRSYGESQNYMRLPEIFMPTEFLHGLYDGGHGAGLYDYWEMMRKHPRCIGGFLWVLADEGVKRVDMDGFIDNQGNFGADGIVGPHHEKEGSYYTIKQLWSPVQVMNTAIDRNFDGKLSVENRYDYLNLNTCRFIWQQVKFPSVTDASNTTTRILKQGEVQGSDVAAHGVGVVDIKTSILPEADALFLTVIDKYGYELWRWTFPVDKLNRETEQFSASSGRVSYTETEKGITVKANGRTFVFSKKDGQLKDVSVNNRKISFANGPRFIGARRADRSLDQFYNHDDEKAKAKDRTYSEFTDAAVFTKLDVKEEGGNLILTANYKLGNLDKAQWTIHPDGMATLDYTYNFSGVVDLMGICFDYPEEQVLSKRWLGAGPYRVWQNRIHGTQYDIWENDYNDPIPGETFTYPEFKGYFGSVSWMSIRTKEGTISLTNETPDSYIGVYQPRDGRDRLLYTLPESGISVLNVIPPVRNKVNSTDLCGPSSQPKWVDGSQTGRLVIRFE
CAGACACTTCCGCAGACAGAGCGGCAATACCTCTCCGGCCACGGATGCGACGACACAGTAGAATGGGACTTTTTCTGTACCGACGGACGTAACTCCGGTCGATGGACGAAAATAGGCGTCCCCTCTTGCTGGGAGTTGCAGGGTTTTGGTACCTATCAGTATGGAATTAGTTTTTATGGTAAAGCCTTTCCCGAAGGCATTGCCGGTGAGAAAGGAATGTATAAATATGAGTTTGAAGTTCCCGAGGAATTTCGTGGCAAGCAGGTCAGCCTTGTGTTCGAAGCATCCATGACCGATACGGAAGTTAAGGTTAACGGACGTAAGGCAGGATCGAAACACCAGGGAGCCTTCTATTGCTTTTCATATAATGTCACGGATTTACTGAAATATGGCAAGAAGAATCAGCTGGAAGTAACAGTTTCCAAGGAGAGTGAGAATGCCAGTGTGAATCTTGCCGAACGGCGCGCCGATTATTGGAACTTTGGCGGTATCTTCCGCCCGGTATTTCTGGAAGTAAAACCTGCCGTCAATCTCCGTCATATTGCTATTGATGCACAAATGGACGGATCATTCCGTGCCAATTGCTACACGAATATCTCCGGTGACGGAATGAGTATCCGTGCACAGATTTTGGACGGTAAAGGGAAGAAACTGGCAGATACCACCGTACCCCTAAAAGCCGGAAGCGACTGGACTACTTTACAATTGAACGTTTCTGCCCCTGCCTTATGGACGGCAGAAACTCCGAATCTTTATAAAGCTCAATTTTCACTGTTGGATAAAGGAGGTAAAGTCCTGCATCATGAGACCGAGACATTCGGTTTCCGTACTATCGAAGTTCGTGAAAGTGACGGATTGTACGTGAACGGGGTGCGTATCAACGTGCGTGGTGTCAACCGTCATAGTTTCCGTCCCGAAAGCGGTCGTACCCTAAGTAAAGCGAAGAATATTGAAGATGTACTTCTGATGAAGGGCATGAATATGAATTCTGTCCGTCTGAGCCACTATCCGGCGGACCCGGAATTTCTGGAAGCATGCGACTCTCTTGGACTCTATGTTATGGATGAACTGGGTGGCTGGCATGGCAAGTACGACACCCCTACGGGAGTACGTCTGATTGAAGGCATGATAGAACGTGATGTGAACCATCCGTCCATTATCTGGTGGAGCAATGGTAATGAAAAAGGCTGGAACATTGAACTGGACGGAGAATTCCATAAATACGATCTGCAGAAACGCCCGGTCATCCATCCGCAAGGTAACTTCTCCGGTTTCGAAACCATGCACTATCGTTCGTATGGAGAAAGCCAGAACTACATGCGCCTGCCGGAAATCTTTATGCCTACTGAATTCCTGCATGGTTTGTACGACGGAGGTCATGGTGCCGGCCTGTATGATTACTGGGAAATGATGCGTAAACATCCGCGTTGTATCGGTGGTTTCCTGTGGGTATTGGCGGATGAAGGCGTGAAGCGCGTGGATATGGACGGGTTCATAGACAATCAGGGAAATTTCGGAGCTGACGGAATTGTAGGCCCTCACCATGAAAAGGAAGGCAGCTATTACACTATCAAGCAGCTATGGAGCCCGGTGCAGGTTATGAATACCGCTATCGACCGGAATTTCGACGGTAAACTCTCTGTGGAGAACCGTTATGATTATCTGAACCTGAACACCTGTCGTTTTATCTGGCAGCAAGTGAAGTTCCCGTCGGTAACGGATGCTTCCAATACAACTACACGGATTCTGAAACAAGGTGAAGTGCAAGGAAGCGATGTAGCAGCCCATGGAGTGGGAGTGGTGGATATCAAGACTTCTATTCTTCCCGAAGCGGATGCTCTTTTCCTGACAGTTATAGATAAATATGGGTATGAACTTTGGCGCTGGACTTTCCCCGTAGATAAACTGAATCGGGAAACAGAACAGTTTTCTGCATCATCCGGCCGTGTATCCTATACGGAAACAGAAAAAGGTATTACGGTAAAAGCAAACGGGCGTACTTTTGTCTTTTCAAAGAAAGACGGGCAGCTGAAAGATGTATCCGTCAATAACCGTAAGATTAGTTTTGCTAACGGTCCCCGTTTTATCGGTGCACGTCGTGCAGACCGTTCCCTAGATCAGTTCTATAATCATGATGACGAAAAAGCCAAGGCAAAGGACCGTACTTACAGTGAATTTACCGATGCGGCAGTCTTCACGAAACTGGATGTGAAAGAAGAGGGGGGGAATCTGATCCTCACCGCTAATTATAAACTGGGTAATTTAGATAAAGCTCAGTGGACAATTCATCCGGACGGCATGGCTACTCTTGATTATACCTACAACTTCTCCGGTGTGGTAGACCTGATGGGTATTTGCTTTGATTACCCTGAAGAACAAGTGCTCAGCAAGCGTTGGTTGGGAGCAGGTCCGTATCGTGTATGGCAGAATCGTATTCATGGCACGCAGTATGATATCTGGGAGAATGATTATAACGATCCTATTCCGGGTGAGACATTCACCTATCCTGAATTCAAGGGATATTTTGGCAGTGTCTCTTGGATGAGTATTCGCACGAAAGAGGGAACCATCAGCCTGACGAATGAAACACCTGATTCCTATATCGGAGTATATCAACCCCGTGATGGTCGTGACCGGTTACTGTATACACTTCCCGAAAGCGGAATTTCTGTTTTGAATGTAATTCCTCCGGTGCGTAATAAAGTAAATTCCACGGACTTGTGCGGTCCTTCTTCACAACCAAAATGGGTGGATGGCTCGCAAACGGGACGCCTTGTTATCCGGTTTGAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,229 total letters Query= CMR200018.111 Length=3195 Score E Sequences producing significant alignments: (Bits) Value wt 5023 0.0 07C_C7_CMR200018.111_C07.seq 1519 0.0 10C_C7_CMR200018.111_C07.seq 24.7 0.59 I V S G * Q F P S R N N F V * L * E G D I H M H H H H H H S Query_1 40 ATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCT 129 1 522 ........... 532 S G V D L W S H P Q F E K G T E N L Y F Q S N A Q T L P Q T Query_1 130 TCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAGACACTTCCGCAGACA 219 2 1 .................. 18 E R Q Y L S G H G C D D T V E W D F F C T D G R N S G R W T Query_1 220 GAGCGGCAATACCTCTCCGGCCACGGATGCGACGACACAGTAGAATGGGACTTTTTCTGTACCGACGGACGTAACTCCGGTCGATGGACG 309 2 19 .......................................................................................... 108 K I G V P S C W E L Q G F G T Y Q Y G I S F Y G K A F P E G Query_1 310 AAAATAGGCGTCCCCTCTTGCTGGGAGTTGCAGGGTTTTGGTACCTATCAGTATGGAATTAGTTTTTATGGTAAAGCCTTTCCCGAAGGC 399 2 109 .......................................................................................... 198 I A G E K G M Y K Y E F E V P E E F R G K Q V S L V F E A S Query_1 400 ATTGCCGGTGAGAAAGGAATGTATAAATATGAGTTTGAAGTTCCCGAGGAATTTCGTGGCAAGCAGGTCAGCCTTGTGTTCGAAGCATCC 489 2 199 .......................................................................................... 288 0 522 ........... 532 M T D T E V K V N G R K A G S K H Q G A F Y C F S Y N V T D Query_1 490 ATGACCGATACGGAAGTTAAGGTTAACGGACGTAAGGCAGGATCGAAACACCAGGGAGCCTTCTATTGCTTTTCATATAATGTCACGGAT 579 2 289 .......................................................................................... 378 L L K Y G K K N Q L E V T V S K E S E N A S V N L A E R R A Query_1 580 TTACTGAAATATGGCAAGAAGAATCAGCTGGAAGTAACAGTTTCCAAGGAGAGTGAGAATGCCAGTGTGAATCTTGCCGAACGGCGCGCC 669 2 379 .......................................................................................... 468 D Y W N F G G I F R P V F L E V K P A V N L R H I A I D A Q Query_1 670 GATTATTGGAACTTTGGCGGTATCTTCCGCCCGGTATTTCTGGAAGTAAAACCTGCCGTCAATCTCCGTCATATTGCTATTGATGCACAA 759 2 469 .......................................................................................... 558 2 1017 .....A........... 1033 2 1873 ........... 1883 M D G S F R A N C Y T N I S G D G M S I R A Q I L D G K G K Query_1 760 ATGGACGGATCATTCCGTGCCAATTGCTACACGAATATCTCCGGTGACGGAATGAGTATCCGTGCACAGATTTTGGACGGTAAAGGGAAG 849 2 559 .......................................................................................... 648 K L A D T T V P L K A G S D W T T L Q L N V S A P A L W T A Query_1 850 AAACTGGCAGATACCACCGTACCCCTAAAAGCCGGAAGCGACTGGACTACTTTACAATTGAACGTTTCTGCCCCTGCCTTATGGACGGCA 939 2 649 .......................................................................................... 738 E T P N L Y K A Q F S L L D K G G K V L H H E T E T F G F R Query_1 940 GAAACTCCGAATCTTTATAAAGCTCAATTTTCACTGTTGGATAAAGGAGGTAAAGTCCTGCATCATGAGACCGAGACATTCGGTTTCCGT 1029 2 739 .......................................................................................... 828 T I E V R E S D G L Y V N G V R I N V R G V N R H S F R P E Query_1 1030 ACTATCGAAGTTCGTGAAAGTGACGGATTGTACGTGAACGGGGTGCGTATCAACGTGCGTGGTGTCAACCGTCATAGTTTCCGTCCCGAA 1119 2 829 .......................................................................................... 918 2 2652 ....... 2658 S G R T L S K A K N I E D V L L M K G M N M N S V R L S H Y Query_1 1120 AGCGGTCGTACCCTAAGTAAAGCGAAGAATATTGAAGATGTACTTCTGATGAAGGGCATGAATATGAATTCTGTCCGTCTGAGCCACTAT 1209 2 919 .......................................................................................... 1008 2 2659 ..... 2663 P A D P E F L E A C D S L G L Y V M D E L G G W H G K Y D T Query_1 1210 CCGGCGGACCCGGAATTTCTGGAAGCATGCGACTCTCTTGGACTCTATGTTATGGATGAACTGGGTGGCTGGCATGGCAAGTACGACACC 1299 2 1009 .......................................................................................... 1098 2 498 .....T........... 514 2 2661 ........... 2671 P T G V R L I E G M I E R D V N H P S I I W W S N G N Q K G Query_1 1300 CCTACGGGAGTACGTCTGATTGAAGGCATGATAGAACGTGATGTGAACCATCCGTCCATTATCTGGTGGAGCAATGGTAATCAGAAAGGC 1389 2 1099 .................................................................................G.A...... 1188 2 568 ........... 558 0 6 ..A.. 10 W N I E L D G E F H K Y D L Q K R P V I H P Q G N F S G F E Query_1 1390 TGGAACATTGAACTGGACGGAGAATTCCATAAATACGATCTGCAGAAACGCCCGGTCATCCATCCGCAAGGTAACTTCTCCGGTTTCGAA 1479 2 1189 .......................................................................................... 1278 2 1281 ......... 1273 2 2323 ............ 2334 0 11 .....-.................................................................................... 99 T M H Y R S Y G E S Q N Y M R L P E I F M P T E F L H G L Query_1 1480 ACCATGCACTATCGTTCG-TATGGAGAAAGCCAGAACTACATGCGCCTGCCGGAAATCTTTATGCCTACTGAATTCCTGCATGGTTTGTA 1568 2 1279 ..................-....................................................................... 1367 2 1272 ... 1270 0 100 ..................A....................................................................... 189 Y D G G H G A G L Y D Y W E M M R K H P R C I G G F L W V L Query_1 1569 CGACGGAGGTCATGGTGCCGGCCTGTATGATTACTGGGAAATGATGCGTAAACATCCGCGTTGTATCGGTGGTTTCCTGTGGGTATTGGC 1658 2 1368 .......................................................................................... 1457 0 190 .......................................................................................... 279 A D E G V K R V D M D G F I D N Q G N F G A D G I V G P H H Query_1 1659 GGATGAAGGCGTGAAGCGCGTGGATATGGACGGGTTCATAGACAATCAGGGAAATTTCGGAGCTGACGGAATTGTAGGCCCTCACCATGA 1748 2 1458 .......................................................................................... 1547 0 280 .......................................................................................... 369 E K E G S Y Y T I K Q L W S P V Q V M N T A I D R N F D G K Query_1 1749 AAAGGAAGGCAGCTATTACACTATCAAGCAGCTATGGAGCCCGGTGCAGGTTATGAATACCGCTATCGACCGGAATTTCGACGGTAAACT 1838 2 1548 .......................................................................................... 1637 0 370 .......................................................................................... 459 L S V E N R Y D Y L N L N T C R F I W Q Q V K F P S V T D A Query_1 1839 CTCTGTGGAGAACCGTTATGATTATCTGAACCTGAACACCTGTCGTTTTATCTGGCAGCAAGTGAAGTTCCCGTCGGTAACGGATGCTTC 1928 2 1638 .......................................................................................... 1727 2 234 ........... 244 0 460 .......................................................................................... 549 S N T T T R I L K Q G E V Q G S D V A A H G V G V V D I K T Query_1 1929 CAATACAACTACACGGATTCTGAAACAAGGTGAAGTGCAAGGAAGCGATGTAGCAGCCCATGGAGTGGGAGTGGTGGATATCAAGACTTC 2018 2 1728 .......................................................................................... 1817 0 550 .......................................................................................... 639 S I L P E A D A L F L T V I D K Y G Y E L W R W T F P V D K Query_1 2019 TATTCTTCCCGAAGCGGATGCTCTTTTCCTGACAGTTATAGATAAATATGGGTATGAACTTTGGCGCTGGACTTTCCCCGTAGATAAA-C 2107 2 1818 ........................................................................................-. 1906 2 477 ........... 487 0 640 ..................................................................................N.....N. 729 L N R E T E Q F S A S S G R V S Y T E T E K G I T V K A Query_1 2108 TG-AATCGGGAAACAGAACAGTTTTCTGCATC-ATCCGGCCGTGTATCCTATACGG-AAACAGAAAAAGG-TATTACGG-TAAAAGC-AA 2191 2 1907 ..-.............................-.......................-.............-........-.......-.. 1990 0 730 ..A.................N...........N.......................A.....N.......T...N....T.......A.. 819 N G R T F V F S K K D G Q L K D V S V N N R K I S F A N Query_1 2192 ACGGGCGTACTTTTGTC-TTTTCAAAGAAAGAC-GGGCAGCT--GAAAGATGTA-TCCG-TCAATAACCGTAAGATTAGTTTTGCT-AAC 2274 2 1991 .................-...............-........--..........-....-..........................-... 2073 0 820 ....N...........NT...............G...N....TGA......N..T....T..........C..N....N.......A... 909 1 863 ....C........... 848 G P R F I G A R R A D R S L D Q F Y N H D D E K A K A Query_1 2275 GGTCCCCGTTTTATCGGTGCACG-TCGTGC--AGACCGTT-CCCTAG-ATCAGTT-CTATAATC--ATGATGA-CGAAAAAGCCAAGGCA 2355 2 2074 .......................-......--........-......-.......-........--.......-................ 2154 0 910 .AN...GN........N......T..N..NCA.A......N...N..A.......N..N.....CANNA....C.A.....N..N..... 999 K D R T Y S E F T D A A V F T K L D V K E E G G N L I Query_1 2356 AA--GGACCGTACTTA-CAGT-GAATTTACCGATGCGGCAGT--CTTCACGAAACTGGATG-TGAAAGAAGAgggggggAATCTGATCCT 2438 2 2155 ..--............-....-....................--.................-............................ 2237 0 1000 ..AG.N...N......N....N..T........A..C..N.NNCN...C.N...T..TN..N.A...A..N.A...... 1078 L T A N Y K L G N L D K A Q W T I H P D G M A T L D Y T Y N Query_1 2439 CACCGCTAATTATAAACTGGGTAATTTAGATAAAGCTCAGTGGACAATTCATCCGGACGGCATGGCTACTCTTGATTATACCTACAACTT 2528 2 2238 .......................................................................................... 2327 2 1261 ..... 1265 0 82 ..... 86 F S G V V D L M G I C F D Y P E E Q V L S K R W L G A G P Y Query_1 2529 CTCCGGTGTGGTAGACCTGATGGGTATTTGCTTTGATTACCCTGAAGAACAAGTGCTCAGCAAGCGTTGGTTGGGAGCAGGTCCGTATCG 2618 2 2328 .......................................................................................... 2417 2 1266 ....... 1272 0 87 ....... 93 1 919 ......... 911 R V W Q N R I H G T Q Y D I W E N D Y N D P I P G E T F T Y Query_1 2619 TGTATGGCAGAATCGTATTCATGGCACGCAGTATGATATCTGGGAGAATGATTATAACGATCCTATTCCGGGTGAGACATTCACCTATCC 2708 2 2418 .......................................................................................... 2507 1 910 .. 909 P E F K G Y F G S V S W M S I R T K E G T I S L T N E T P D Query_1 2709 TGAATTCAAGGGATATTTTGGCAGTGTCTCTTGGATGAGTATTCGCACGAAAGAGGGAACCATCAGCCTGACGAATGAAACACCTGATTC 2798 2 2508 .......................................................................................... 2597 S Y I G V Y Q P R D G R D R L L Y T L P E S G I S V L N V I Query_1 2799 CTATATCGGAGTATATCAACCCCGTGATGGTCGTGACCGGTTACTGTATACACTTCCCGAAAGCGGAATTTCTGTTTTGAATGTAATTCC 2888 2 2598 .......................................................................................... 2687 2 912 ............ 923 2 1019 ........... 1029 P P V R N K V N S T D L C G P S S Q P K W V D G S Q T G R L Query_1 2889 TCCGGTGCGTAATAAAGTAAATTCCACGGACTTGTGCGGTCCTTCTTCACAACCAAAATGGGTGGATGGCTCGCAAACGGGACGCCTTGT 2978 2 2688 .......................................................................................... 2777 V I R F E Query_1 2979 TATCCGGTTTGAA 2991 2 2778 ............. 2790 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 16440975 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 5,229 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200027.105 (CMR200027.20-824.P(824).E426A.pMCSG68)
QRSEYLLEKNWKFMKGEAPEAMKPEFDDRKWETVTVPHDWAIFGPFDRSNDLQEVAVTQNFEKKASVKTGRTGGLPYVGIGWYRTRFDAPVNQQTTLVFDGAMSEARVYVNGQEACFWPFGYNSFHCDVTGLLNKDGKNNTLAVRLENKPQSSRWYPGAGLYRNVRVVSTDKVHVPVWGTQLTTPHVSDEYASVRLLTTIANDEERDIRIVTEIISPDGKVVATKDNTRKINHGQPFEQNFLVNAPCLWSPETPYLYKAVSKIYADGKQTDEYTTRFGIRSIEIIADKGFFLNGKHRKFQGVCNHHDLGPLGAAINVAALRRQLTMLKDMGCDAIRTAHNMPAPELVQLCDEMGFMMMLEPFDEWDIAKCENGYHRYFNEWAERDMINMLHQFRNNPCVVMWSIGNEVPTQCSPVGYKVASFLQDICHREDPTRPVTCGMDQVTCVLANGFAAMIDVPGFNYRAHRYLEAYELLPQNIVLGSETSSTVSSRGVYKFPVEKRGDAKYDDHQSSGYDLEHCAWSNVPDEDFALADDYDWTIGQFVWTGFDYLGEPSPYDTDAWPSHSSLFGIIDLASLPKDRYYLYRSLWNKNVNTLHILPHWTWPGREGENTPVFVYTNYPAAELFVNGKSYGKQHKLTAEESKAIQDKDTLALQRRYRLMWMDVPYEPGEVKVVAYDASGKPAEEKVVRTSGKPHHLEVIADRDQLTADGKDLAYITVRVVDKDGNLCPADNRLVNFTVKGAGRYRAAANGDATSLDLFHLPKMPAFSGQLTAIVQMTEQPGEIIFEAKAKGVKSGKLVLRSVRE
CAGCGCAGTGAGTATCTACTTGAAAAGAACTGGAAGTTCATGAAGGGGGAAGCTCCGGAAGCCATGAAGCCGGAATTTGACGACCGGAAGTGGGAAACCGTAACCGTGCCTCACGACTGGGCCATTTTTGGTCCCTTCGATCGCAGCAACGATTTGCAGGAAGTGGCGGTAACGCAGAACTTCGAGAAGAAAGCTTCCGTCAAGACCGGACGTACCGGTGGACTTCCTTATGTTGGCATCGGATGGTATCGTACTAGGTTCGATGCCCCCGTCAATCAACAGACGACACTTGTCTTTGATGGTGCCATGAGCGAAGCCCGTGTATATGTCAATGGACAAGAAGCATGCTTCTGGCCATTTGGTTATAATTCTTTCCATTGTGATGTCACCGGACTTTTGAATAAAGACGGTAAAAACAATACGCTTGCCGTGCGTTTGGAAAATAAACCACAATCTTCCCGTTGGTATCCTGGCGCAGGACTTTATCGCAATGTGCGTGTAGTGAGTACCGATAAAGTACATGTTCCTGTATGGGGTACTCAGCTGACTACTCCTCATGTTTCTGATGAGTATGCTTCAGTACGTCTGTTGACCACTATTGCCAATGATGAAGAAAGAGATATCCGTATCGTGACAGAGATAATCTCTCCCGATGGGAAAGTCGTTGCAACGAAGGATAATACCCGTAAGATTAATCATGGTCAGCCTTTTGAACAAAACTTCCTGGTGAATGCTCCTTGCTTGTGGTCGCCGGAGACACCTTATTTATATAAAGCTGTTTCTAAAATCTATGCCGATGGCAAGCAAACGGATGAATACACTACTCGTTTCGGCATCCGCAGCATAGAAATCATTGCCGACAAAGGATTTTTCCTGAACGGTAAGCATCGCAAGTTCCAGGGGGTGTGCAATCACCACGATCTTGGTCCGTTAGGCGCTGCCATCAATGTTGCTGCATTGCGCCGTCAACTTACGATGCTGAAAGATATGGGTTGTGATGCCATCCGCACCGCTCACAATATGCCGGCACCGGAGTTAGTGCAACTTTGTGATGAAATGGGTTTTATGATGATGCTGGAACCTTTCGACGAATGGGACATTGCCAAATGTGAGAATGGCTATCACCGTTATTTCAACGAGTGGGCAGAACGTGATATGATAAATATGTTGCATCAGTTCCGCAACAATCCTTGTGTCGTAATGTGGAGTATCGGTAATGAAGTTCCTACCCAATGTAGTCCCGTAGGCTATAAAGTCGCTTCTTTCTTGCAGGATATCTGTCATCGTGAAGATCCGACACGTCCTGTTACTTGCGGCATGGATCAGGTGACTTGTGTTCTTGCTAATGGTTTTGCCGCCATGATTGATGTGCCCGGTTTTAATTATCGCGCACACCGTTATCTGGAAGCTTATGAACTGTTGCCGCAGAATATAGTACTTGGTTCTGAAACATCCTCTACCGTTAGTTCTCGTGGCGTATATAAATTTCCTGTAGAGAAACGCGGGGATGCGAAGTACGATGATCACCAGTCTTCCGGATATGACTTGGAGCATTGTGCCTGGTCTAATGTTCCAGATGAAGATTTTGCTTTAGCGGATGATTATGACTGGACTATCGGTCAATTCGTTTGGACAGGATTCGATTATCTGGGTGAGCCTTCTCCTTATGATACGGATGCATGGCCAAGTCATAGCTCTTTGTTTGGTATCATTGACCTTGCCAGTTTGCCAAAAGACCGCTACTATCTGTACCGTAGTCTTTGGAATAAGAATGTGAATACACTCCATATACTTCCTCACTGGACATGGCCGGGTAGGGAAGGAGAGAATACTCCTGTCTTTGTTTACACAAACTATCCTGCTGCCGAACTTTTCGTTAATGGAAAAAGCTATGGTAAACAGCATAAACTGACAGCCGAAGAGAGTAAAGCTATTCAGGACAAAGATACACTTGCCCTCCAGCGTCGTTACCGCCTGATGTGGATGGACGTTCCTTATGAGCCGGGTGAAGTGAAAGTGGTGGCTTACGATGCTTCCGGCAAACCTGCTGAAGAAAAAGTAGTTCGTACTTCCGGCAAACCTCATCATCTGGAAGTCATTGCTGACCGTGACCAACTCACTGCCGATGGTAAAGATTTGGCATACATCACTGTTCGTGTGGTTGATAAAGACGGAAACCTTTGTCCTGCTGATAATCGTCTTGTAAACTTTACGGTGAAAGGCGCGGGGCGTTATCGTGCTGCCGCTAATGGAGATGCAACTTCACTTGATTTATTCCACTTGCCGAAGATGCCCGCTTTCAGTGGTCAGCTGACAGCCATTGTTCAAATGACCGAACAGCCCGGTGAAATTATTTTCGAGGCTAAGGCTAAAGGGGTGAAATCTGGTAAGCTTGTGCTGAGGTCTGTTAGAGAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,570 total letters Query= CMR200027.105 Length=2820 Score E Sequences producing significant alignments: (Bits) Value wt 4347 0.0 07D_D7_CMR200027.105_D07.seq 1665 0.0 10D_D7_CMR200027.105_D07.seq 1575 0.0 I V S G * Q F P S R N N F V * L * E G D I H M H H H H H H S Query_1 40 ATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCT 129 2 1020 ........... 1010 S G V D L W S H P Q F E K G T E N L Y F Q S N A Q R S E Y L Query_1 130 TCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAGCGCAGTGAGTATCTA 219 2 1 .................. 18 L E K N W K F M K G E A P E A M K P E F D D R K W E T V T V Query_1 220 CTTGAAAAGAACTGGAAGTTCATGAAGGGGGAAGCTCCGGAAGCCATGAAGCCGGAATTTGACGACCGGAAGTGGGAAACCGTAACCGTG 309 2 19 .......................................................................................... 108 P H D W A I F G P F D R S N D L Q E V A V T Q N F E K K A S Query_1 310 CCTCACGACTGGGCCATTTTTGGTCCCTTCGATCGCAGCAACGATTTGCAGGAAGTGGCGGTAACGCAGAACTTCGAGAAGAAAGCTTCC 399 2 109 .......................................................................................... 198 V K T G R T G G L P Y V G I G W Y R T R F D A P V N Q Q T T Query_1 400 GTCAAGACCGGACGTACCGGTGGACTTCCTTATGTTGGCATCGGATGGTATCGTACTAGGTTCGATGCCCCCGTCAATCAACAGACGACA 489 2 199 .......................................................................................... 288 L V F D G A M S E A R V Y V N G Q E A C F W P F G Y N S F Query_1 490 CTTGTCTTTGATGGTGCCATGAGCGAAGCCCGTGTATATGTCAATGGACAAGAAGCATGCTTC-TGGCCATTTGGTTATAATTCTTTCCA 578 2 289 ...............................................................-.......................... 377 2 359 .....C..-............T..T..... 331 H C D V T G L L N K D G K N N T L A V R L E N K P Q S S R W Query_1 579 TTGTGATGTCACCGGACTTTTGAATAAAGACGGTAAAAACAATACGCTTGCCGTGCGTTTGGAAAATAAACCACAATCTTCCCGTTGGTA 668 2 378 .......................................................................................... 467 Y P G A G L Y R N V R V V S T D K V H V P V W G T Q L T T P Query_1 669 TCCTGGCGCAGGACTTTATCGCAATGTGCGTGTAGTGAGTACCGATAAAGTACATGTTCCTGTATGGGGTACTCAGCTGACTACTCCTCA 758 2 468 .......................................................................................... 557 H V S D E Y A S V R L L T T I A N D E E R D I R I V T E I I Query_1 759 TGTTTCTGATGAGTATGCTTCAGTACGTCTGTTGACCACT-ATTGCCAATGATGAAGAAAGAGATATCCGTATCGTGACAGAGATAATCT 847 2 558 ........................................-................................................. 646 1 961 ....N........N..N..W..K.....................N........................ 893 S P D G K V V A T K D N T R K I N H G Q P F E Q N F L V N A Query_1 848 CTCCCGATGGGAAAGTCGTTGCAACGAAGGATAATACCCGTAAGATTAATCATGGTCAGCCTTTTGAACAAAACTTCCT-GGTGAATGCT 936 2 647 ...............................................................................-.......... 735 2 673 ............ 662 1 892 ..........-........-.....................................................N.....N........N. 805 P C L W S P E T P Y L Y K A V S K I Y A D G K Q T D E Y T T Query_1 937 CCTTGCTTGTGGTCGCCGGAGACACCTTATTTATATAAAGCTGTTTCTAAAATCTATGCCGATGGCAAGCAAACGGATGAATACACTACT 1026 2 736 .......................................................................................... 825 1 804 .................A........................................................................ 715 R F G I R S I E I I A D K G F F L N G K H R K F Q G V C N H Query_1 1027 CGTTTCGGCATCCGCAGCATAGAAATCATTGCCGACAAAGGATTTTTCCTGAACGGTAAGCATCGCAAGTTCCAGGGGGTGTGCAATCAC 1116 2 826 .......................................................................................... 915 1 714 .......................................................................................... 625 H D L G P L G A A I N V A A L R R Q L T M L K D M G C D A I Query_1 1117 CACGATCTTGGTCCGTTAGGCGCTGCCATCAATGTTGCTGCATTGCGCCGTCAACTTACGATGCTGAAAGATATGGGTTGTGATGCCATC 1206 2 916 .......................................................................................... 1005 1 624 .......................................................................................... 535 R T A H N M P A P E L V Q L C D E M G F M M M L E P F D E W Query_1 1207 CGCACCGCTCACAATATGCCGGCACCGGAGTTAGTGCAACTTTGTGATGAAATGGGTTTTATGATGATGCTGGAACCTTTCGACGAATGG 1296 2 1006 .......................................................................................... 1095 1 534 .......................................................................................... 445 D I A K C E N G Y H R Y F N E W A E R D M I N M L H Q F R Query_1 1297 GACATTGCCAAATGTGAGAATGGCTATCACCG-TTATTTCAACGAGTGGGCAGAACGTGATATGATAAATATGTTGCATCAGTTCCGCAA 1385 2 1096 ................................-......................................................... 1184 0 18 ....N...N.......N-................................................ 82 1 444 ................................-......................................................... 356 N N P C V V M W S I G N A V P T Q C S P V G Y K V A S F L Q Query_1 1386 CAATCCTTGTGTCGTAATGTGGAGTATCGGTAATGCTGTTCCTACCCAATGTAGTCCCGTAGGCTATAAAGTCGCTTCTTTCTTGCAGGA 1475 2 1185 ...................................AA..................................................... 1274 0 83 .......................................................................................... 172 1 355 ..................................CAG..................................................... 266 D I C H R E D P T R P V T C G M D Q V T C V L A N G F A A M Query_1 1476 TATCTGTCATCGTGAAGATCCGACACGTCCTGTTACTTGCGGCATGGATCAGGTGACTTGTGTTCTTGCTAATGGTTTTGCCGCCATGAT 1565 2 1275 .......................................................................................... 1364 0 173 .......................................................................................... 262 1 265 ....................................................................N..................... 176 I D V P G F N Y R A H R Y L E A Y E L L P Q N I V L G S E T Query_1 1566 TGATGTGCCCGGTTTTAATTATCGCGCACACCGTTATCTGGAAGCTTATGAACTGTTGCCGCAGAATATAGTACTTGGTTCTGAAACATC 1655 2 1365 .......................................................................................... 1454 0 263 .......................................................................................... 352 1 175 .................................................N.N....N...............................NT 86 S S T V S S R G V Y K F P V E K R G D A K Y D D H Q S S G Y Query_1 1656 CTCTACCGTTAGTTCTCGTGGCGTATATAAATTTCCTGTAGAGAAACGCGGGGATGCGAAGTACGATGATCACCAGTCTTCCGGATATGA 1745 2 1455 .......................................................................................... 1544 0 353 .......................................................................................... 442 1 85 TC.GT..T...................................................... 24 D L E H C A W S N V P D E D F A L A D D Y D W T I G Q F V W Query_1 1746 CTTGGAGCATTGTGCCTGGTCTAATGTTCCAGATGAAGATTTTGCTTTAGCGGATGATTATGACTGGACTATCGGTCAATTCGTTTGGAC 1835 2 1545 .......................................................................................... 1634 0 443 .......................................................................................... 532 T G F D Y L G E P S P Y D T D A W P S H S S L F G I I D L A Query_1 1836 AGGATTCGATTATCTGGGTGAGCCTTCTCCTTATGATACGGATGCATGGCCAAGTCATAGCTCTTTGTTTGGTATCATTGACCTTGCCAG 1925 2 1635 .......................................................................................... 1724 0 533 .......................................................................................... 622 S L P K D R Y Y L Y R S L W N K N V N T L H I L P H W T W P Query_1 1926 TTTGCCAAAAGACCGCTACTATCTGTACCGTAGTCTTTGGAATAAGAATGTGAATACACT-CCATATACTTCCTCACTGGACATGGCCGG 2014 2 1725 ............................................................-............................. 1813 0 623 ............................................................C............................. 712 G R E G E N T P V F V Y T N Y P A A E L F V N G K S Y G K Query_1 2015 G-TAGGGAAGGAGAGAATACTCCTGTCTTTG-TTTACACAAACTAT-CCTGCTGCCGAAC-TTTTCGTTAATGGAAAAAGCTATGGTAAA 2100 2 1814 .-.............................-..............-.............-............................. 1899 0 713 .N.............................T..............C.............T..........................N.. 802 Q H K L T A E E S K A I Q D K D T L A L Q R R Y R L M W Query_1 2101 CAGCATAAACTGACAGCCGAAGAGAG-TAAAGC-TA-TTCAGGAC-AAAGATACACTTGCCCTCCAGCGTCGTTACCGCCTGATGTGGAT 2186 2 1900 ..........................-......-..-........-............................................ 1985 0 803 .N.......................NT......Y..T........N.................-.NN....N.................. 891 M D V P Y E P G E V K V V A Y D A S G K P A E E K V V R T S Query_1 2187 GGACGTTCCTTATGAGCCGGGTGAAGTGAAAGTGGTGGCTTACGATG-CTTCCGGCAAACCTGCTGAAGAAAAAGTAGTTCGTAC-TTCC 2274 2 1986 ...............................................-.....................................-.... 2073 2 970 .........-.. 980 0 892 ....N.N.T.NN......-..N....N....N...............NN............................-N......T.... 979 G K P H H L E V I A D R D Q L T A D G K D L A Y I T V R Query_1 2275 GGCAAA-CCTC-ATCA-TCTGGAAGTCATTGCTGACCGTGACCAACTCACTGCCGATGGTAAAGATTTGGCATACAT-CACTGTTCGTGT 2360 2 2074 ......-....-....-............................................................-............ 2159 0 980 ......NN...N....T.......N....G.....-..N....-....C...G.......-.........NN.....C......N....G 1066 V V D K D G N L C P A D N R L V N F T V K G A G R Y R A Query_1 2361 GGTTGATAAAGACGGAAA-CCTTTGTCCTGCTGATAAT-CGTCTTGTAAAC--TTTAC-GGTGAAA-GGCGCG-GGGCGTTATCGT-GCT 2442 2 2160 ..................-...................-............--.....-.......-......-............-... 2241 0 1067 T.G...A...N..N....N.........G.....N...C....N......MAN.....N..N....G..N.N.N.......N.NNNG... 1156 A A N G D A T S L D L F H L P K M P A F S G Q L T A I V Q M Query_1 2443 GCCGCTAATGGAGATGCAACTTCACTTGATTTATTCCACTTGCCGAAGATGCCCGCTTTCAGTGGTCAGCTGACAGCCATTGTTCAAATG 2532 2 2242 .......................................................................................... 2331 0 1157 ...... 1162 T E Q P G E I I F E A K A K G V K S G K L V L R S V R E Query_1 2533 ACCGAACAGCCCGGTGAAATTATTTTCGAGGCTAAGGCTAAAGGGGTGAAATCTGGTAAGCTTGTGCTGAGGTCTGTTAGAGAG 2616 2 2332 .................................................................................... 2415 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12666757 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 4,570 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200027.106 (CMR200027.20-824.P(824).E426Q.pMCSG68)
QRSEYLLEKNWKFMKGEAPEAMKPEFDDRKWETVTVPHDWAIFGPFDRSNDLQEVAVTQNFEKKASVKTGRTGGLPYVGIGWYRTRFDAPVNQQTTLVFDGAMSEARVYVNGQEACFWPFGYNSFHCDVTGLLNKDGKNNTLAVRLENKPQSSRWYPGAGLYRNVRVVSTDKVHVPVWGTQLTTPHVSDEYASVRLLTTIANDEERDIRIVTEIISPDGKVVATKDNTRKINHGQPFEQNFLVNAPCLWSPETPYLYKAVSKIYADGKQTDEYTTRFGIRSIEIIADKGFFLNGKHRKFQGVCNHHDLGPLGAAINVAALRRQLTMLKDMGCDAIRTAHNMPAPELVQLCDEMGFMMMLEPFDEWDIAKCENGYHRYFNEWAERDMINMLHQFRNNPCVVMWSIGNEVPTQCSPVGYKVASFLQDICHREDPTRPVTCGMDQVTCVLANGFAAMIDVPGFNYRAHRYLEAYELLPQNIVLGSETSSTVSSRGVYKFPVEKRGDAKYDDHQSSGYDLEHCAWSNVPDEDFALADDYDWTIGQFVWTGFDYLGEPSPYDTDAWPSHSSLFGIIDLASLPKDRYYLYRSLWNKNVNTLHILPHWTWPGREGENTPVFVYTNYPAAELFVNGKSYGKQHKLTAEESKAIQDKDTLALQRRYRLMWMDVPYEPGEVKVVAYDASGKPAEEKVVRTSGKPHHLEVIADRDQLTADGKDLAYITVRVVDKDGNLCPADNRLVNFTVKGAGRYRAAANGDATSLDLFHLPKMPAFSGQLTAIVQMTEQPGEIIFEAKAKGVKSGKLVLRSVRE
CAGCGCAGTGAGTATCTACTTGAAAAGAACTGGAAGTTCATGAAGGGGGAAGCTCCGGAAGCCATGAAGCCGGAATTTGACGACCGGAAGTGGGAAACCGTAACCGTGCCTCACGACTGGGCCATTTTTGGTCCCTTCGATCGCAGCAACGATTTGCAGGAAGTGGCGGTAACGCAGAACTTCGAGAAGAAAGCTTCCGTCAAGACCGGACGTACCGGTGGACTTCCTTATGTTGGCATCGGATGGTATCGTACTAGGTTCGATGCCCCCGTCAATCAACAGACGACACTTGTCTTTGATGGTGCCATGAGCGAAGCCCGTGTATATGTCAATGGACAAGAAGCATGCTTCTGGCCATTTGGTTATAATTCTTTCCATTGTGATGTCACCGGACTTTTGAATAAAGACGGTAAAAACAATACGCTTGCCGTGCGTTTGGAAAATAAACCACAATCTTCCCGTTGGTATCCTGGCGCAGGACTTTATCGCAATGTGCGTGTAGTGAGTACCGATAAAGTACATGTTCCTGTATGGGGTACTCAGCTGACTACTCCTCATGTTTCTGATGAGTATGCTTCAGTACGTCTGTTGACCACTATTGCCAATGATGAAGAAAGAGATATCCGTATCGTGACAGAGATAATCTCTCCCGATGGGAAAGTCGTTGCAACGAAGGATAATACCCGTAAGATTAATCATGGTCAGCCTTTTGAACAAAACTTCCTGGTGAATGCTCCTTGCTTGTGGTCGCCGGAGACACCTTATTTATATAAAGCTGTTTCTAAAATCTATGCCGATGGCAAGCAAACGGATGAATACACTACTCGTTTCGGCATCCGCAGCATAGAAATCATTGCCGACAAAGGATTTTTCCTGAACGGTAAGCATCGCAAGTTCCAGGGGGTGTGCAATCACCACGATCTTGGTCCGTTAGGCGCTGCCATCAATGTTGCTGCATTGCGCCGTCAACTTACGATGCTGAAAGATATGGGTTGTGATGCCATCCGCACCGCTCACAATATGCCGGCACCGGAGTTAGTGCAACTTTGTGATGAAATGGGTTTTATGATGATGCTGGAACCTTTCGACGAATGGGACATTGCCAAATGTGAGAATGGCTATCACCGTTATTTCAACGAGTGGGCAGAACGTGATATGATAAATATGTTGCATCAGTTCCGCAACAATCCTTGTGTCGTAATGTGGAGTATCGGTAATGAAGTTCCTACCCAATGTAGTCCCGTAGGCTATAAAGTCGCTTCTTTCTTGCAGGATATCTGTCATCGTGAAGATCCGACACGTCCTGTTACTTGCGGCATGGATCAGGTGACTTGTGTTCTTGCTAATGGTTTTGCCGCCATGATTGATGTGCCCGGTTTTAATTATCGCGCACACCGTTATCTGGAAGCTTATGAACTGTTGCCGCAGAATATAGTACTTGGTTCTGAAACATCCTCTACCGTTAGTTCTCGTGGCGTATATAAATTTCCTGTAGAGAAACGCGGGGATGCGAAGTACGATGATCACCAGTCTTCCGGATATGACTTGGAGCATTGTGCCTGGTCTAATGTTCCAGATGAAGATTTTGCTTTAGCGGATGATTATGACTGGACTATCGGTCAATTCGTTTGGACAGGATTCGATTATCTGGGTGAGCCTTCTCCTTATGATACGGATGCATGGCCAAGTCATAGCTCTTTGTTTGGTATCATTGACCTTGCCAGTTTGCCAAAAGACCGCTACTATCTGTACCGTAGTCTTTGGAATAAGAATGTGAATACACTCCATATACTTCCTCACTGGACATGGCCGGGTAGGGAAGGAGAGAATACTCCTGTCTTTGTTTACACAAACTATCCTGCTGCCGAACTTTTCGTTAATGGAAAAAGCTATGGTAAACAGCATAAACTGACAGCCGAAGAGAGTAAAGCTATTCAGGACAAAGATACACTTGCCCTCCAGCGTCGTTACCGCCTGATGTGGATGGACGTTCCTTATGAGCCGGGTGAAGTGAAAGTGGTGGCTTACGATGCTTCCGGCAAACCTGCTGAAGAAAAAGTAGTTCGTACTTCCGGCAAACCTCATCATCTGGAAGTCATTGCTGACCGTGACCAACTCACTGCCGATGGTAAAGATTTGGCATACATCACTGTTCGTGTGGTTGATAAAGACGGAAACCTTTGTCCTGCTGATAATCGTCTTGTAAACTTTACGGTGAAAGGCGCGGGGCGTTATCGTGCTGCCGCTAATGGAGATGCAACTTCACTTGATTTATTCCACTTGCCGAAGATGCCCGCTTTCAGTGGTCAGCTGACAGCCATTGTTCAAATGACCGAACAGCCCGGTGAAATTATTTTCGAGGCTAAGGCTAAAGGGGTGAAATCTGGTAAGCTTGTGCTGAGGTCTGTTAGAGAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,885 total letters Query= CMR200027.106 Length=2820 Score E Sequences producing significant alignments: (Bits) Value wt 4347 0.0 10E_E7_CMR200027.106_E07.seq 1703 0.0 07E_E7_CMR200027.106_E07.seq 1476 0.0 I V S G * Q F P S R N N F V * L * E G D I H M H H H H H H S Query_1 40 ATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCT 129 2 1020 ........... 1010 S G V D L W S H P Q F E K G T E N L Y F Q S N A Q R S E Y L Query_1 130 TCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAGCGCAGTGAGTATCTA 219 2 1 .................. 18 L E K N W K F M K G E A P E A M K P E F D D R K W E T V T V Query_1 220 CTTGAAAAGAACTGGAAGTTCATGAAGGGGGAAGCTCCGGAAGCCATGAAGCCGGAATTTGACGACCGGAAGTGGGAAACCGTAACCGTG 309 2 19 .......................................................................................... 108 P H D W A I F G P F D R S N D L Q E V A V T Q N F E K K A S Query_1 310 CCTCACGACTGGGCCATTTTTGGTCCCTTCGATCGCAGCAACGATTTGCAGGAAGTGGCGGTAACGCAGAACTTCGAGAAGAAAGCTTCC 399 2 109 .......................................................................................... 198 V K T G R T G G L P Y V G I G W Y R T R F D A P V N Q Q T T Query_1 400 GTCAAGACCGGACGTACCGGTGGACTTCCTTATGTTGGCATCGGATGGTATCGTACTAGGTTCGATGCCCCCGTCAATCAACAGACGACA 489 2 199 .......................................................................................... 288 L V F D G A M S E A R V Y V N G Q E A C F W P F G Y N S F Query_1 490 CTTGTCTTTGATGGTGCCATGAGCGAAGCCCGTGTATATGTCAATGGACAAGAAGCATGCTTC-TGGCCATTTGGTTATAATTCTTTCCA 578 2 289 ...............................................................-.......................... 377 2 359 .....C..-............T..T..... 331 H C D V T G L L N K D G K N N T L A V R L E N K P Q S S R W Query_1 579 TTGTGATGTCACCGGACTTTTGAATAAAGACGGTAAAAACAATACGCTTGCCGTGCGTTTGGAAAATAAACCACAATCTTCCCGTTGGTA 668 2 378 .......................................................................................... 467 Y P G A G L Y R N V R V V S T D K V H V P V W G T Q L T T P Query_1 669 TCCTGGCGCAGGACTTTATCGCAATGTGCGTGTAGTGAGTACCGATAAA-GTAC-ATGTTCCTGTATGGGGTACTCAGCTGACTACTCCT 756 2 468 .................................................-....-................................... 555 1 1045 ...NT.......N....N.N.............N-.......N........-. 995 H V S D E Y A S V R L L T T I A N D E E R D I R I V T E I Query_1 757 CATGTTTCTGATGAGTATGCTTCAGTACGTCTGTTGACC-ACTATTGCCAATGATGAAGAAA-GAGATATCCGTATCGTGACAGAGATAA 844 2 556 .......................................-......................-........................... 643 1 994 ................................N......C......................A........................... 905 I S P D G K V V A T K D N T R K I N H G Q P F E Q N F L V Query_1 845 TCTCTCCCGATGGGAAA-GTCGTTGCAACGAAGGATAATACCCGTAAGATTAATCATGGTCAGCCTTTTGAACAAAACTTCCTGGTGAA- 932 2 644 .................-.......................................................................- 731 2 673 ............ 662 1 904 .................A....G..................................................................A 815 N A P C L W S P E T P Y L Y K A V S K I Y A D G K Q T D E Y Query_1 933 TGCTCCTTGCTTGTGGTCGCCGG-AGACACCTTATTTATATAAAGCTGTTTCTAAAA-TCTATGCCGATGGCAAGCAAACGGATGAATAC 1020 2 732 .......................-.................................-................................ 819 1 814 ......C................G.................................A................................ 725 T T R F G I R S I E I I A D K G F F L N G K H R K F Q G V Query_1 1021 ACTACTCGTTTCGGCATCCGCAGCATAGAAA-TCATTGCCGACAAAGGATTTTTCCTGAACGGTAAGCATCGCAAGTTCCAGGGGGTGTG 1109 2 820 ...............................-.......................................................... 908 1 724 ...............................A.......................................................... 635 C N H H D L G P L G A A I N V A A L R R Q L T M L K D M G C Query_1 1110 CAATCACCACGATCTTGGTCCGTTAGGCGCTGCCATCAATGTTGCTGCATTGCGCCGTCAACTTACGATGCTGAAAGATATGGGTTGTGA 1199 2 909 .......................................................................................... 998 1 634 .......................................................................................... 545 D A I R T A H N M P A P E L V Q L C D E M G F M M M L E P F Query_1 1200 TGCCATCCGCACCGCTCACAATATGCCGGCACCGGAGTTAGTGCAACTTTGTGATGAAATGGGTTTTATGATGATGCTGGAACCTTTCGA 1289 2 999 .......................................................................................... 1088 1 544 .......................................................................................... 455 D E W D I A K C E N G Y H R Y F N E W A E R D M I N M L H Q Query_1 1290 CGAATGGGACATTGCCAAATGTGAGAATGGCTATCACCGTTATTTCAACGAGTGGGCAGAACGTGATATGATAAATATGTTGCATCAG-T 1378 2 1089 ........................................................................................-. 1177 1 454 ........................................................................................-. 366 0 9 .................................................A. 59 F R N N P C V V M W S I G N Q V P T Q C S P V G Y K V A S F Query_1 1379 TCCGCAACAATCCTTGTGTCGTAATGTGGAGTATCGGTAATCAGGTTCCTACCCAATGTAGTCCCGTAGGCTATAAAGTCGCTTCTTTCT 1468 2 1178 .........................................G.A.............................................. 1267 1 365 .........................................G.A.............................................. 276 0 60 ...N.TTTN.........................................................N....................... 149 L Q D I C H R E D P T R P V T C G M D Q V T C V L A N G F A Query_1 1469 TGCAGGATATCTGTCATCGTGAAGATCCGACACGTCCTGTTACTTGCGGCATGGATCAGGTGACTTGTGTTCTTGCTAATGGTTTTGCCG 1558 2 1268 .......................................................................................... 1357 1 275 .......................................................................................... 186 0 150 .......................................................................................... 239 A M I D V P G F N Y R A H R Y L E A Y E L L P Q N I V L G S Query_1 1559 CCATGATTGATGTGCCCGGTTTTAATTATCGCGCACACCGTTATCTGGAAGCTTATGAACTGTTGCCGCAGAATATAGTACTTGGTTCTG 1648 2 1358 .......................................................................................... 1447 1 185 .......................................................................................... 96 0 240 .......................................................................................... 329 E T S S T V S S R G V Y K F P V E K R G D A K Y D D H Q S S Query_1 1649 AAACATCCTCTACCGTTAGTTCTCGTGGCGTATATAAATTTCCTGTAGAGAAACGCGGGGATGCGAAGTAC-GATGATCACCAGTCTTCC 1737 2 1448 .......................................................................-.................. 1536 1 95 CT..........................................................N.N........N...NN..... 14 0 330 .......................................................................-.................. 418 G Y D L E H C A W S N V P D E D F A L A D D Y D W T I G Q F Query_1 1738 GGATATGACTTGGAGCATTGTGCCTGGTCTAATGTTCCAGATGAAGATTTTGCTTTAGCGGATGATTATGACTGGACTATCGGTCAATTC 1827 2 1537 .......................................................................................... 1626 0 419 .......................................................................................... 508 V W T G F D Y L G E P S P Y D T D A W P S H S S L F G I I D Query_1 1828 GTTTGGACAGGATTCGATTATCTGGGTGAGCCTTCTCCTTATGATACGGATGCATGGCCAAGTCATAGCTCTTTGTTTGGTATCATTGAC 1917 2 1627 .......................................................................................... 1716 0 509 .........................................................N................................ 598 L A S L P K D R Y Y L Y R S L W N K N V N T L H I L P H W T Query_1 1918 CTTGCCAGTTTGCCAAAAGACCGCTACTATCTGTACCGTAGTCTTTGGAATAAGAATGTGAATACACTCCATATACTTCCTCACTGGACA 2007 2 1717 .......................................................................................... 1806 0 599 .......................................................................................... 688 W P G R E G E N T P V F V Y T N Y P A A E L F V N G K S Y G Query_1 2008 TGGCCGGGTAGGGAAGGAGAGAATACTCCTGTCTTTGTTTACACAAACTATCCTGCTGCCGAACTTTTCGTTAATGGAAAAAGCTATGGT 2097 2 1807 .......................................................................................... 1896 0 689 .....N.......C........................N...................................N....N.......... 778 K Q H K L T A E E S K A I Q D K D T L A L Q R R Y R L M Query_1 2098 AAACAGC-ATAAACTGACAGCCGAAGAGAGT-AAAG-CTATT-CAGGACAAAGATACAC-TTGCCCTCCAGCGTC-GTTACCGCCTGATG 2181 2 1897 .......-.......................-....-.....-................-...............-.............. 1980 0 779 .......N.........N.............N....C.N...C.NC........N..C.T...............N..AN.......... 868 W M D V P Y E P G E V K V V A Y D A S G K P A E E K V Query_1 2182 TGGATGG-ACGTTCCTTATGAGC-CGGGTGAAGTG-AAAGTGGTGGCTTA-CGATGCTTCC--GGCAAACCTGC-TGAAGAAAAAGTAGT 2264 2 1981 .......-...............-...........-..............-..........--...........-............... 2063 2 970 ....-....... 980 0 869 N......A..N.N...N..N.N.N....N......A...CN..N......C...A......CA.CN.....N.NT....A.....N.... 958 V R T S G K P H H L E V I A D R D Q L T A D G K D L A Y I T Query_1 2265 TCG-TACTTCCGGCAAACCTCATCATCTGGAAGTCATTGCTGACCGTGACCAACTCACTGCCGATGGTAAAGATTTGGCATACATCACTG 2353 2 2064 ...-...................................................................................... 2152 0 959 ...N...C...C.....N.. 978 V R V V D K D G N L C P A D N R L V N F T V K G A G R Y R A Query_1 2354 TTCGTGTGGTTGATAAAGACGGAAACCTTTGTCCTGCTGATAATCGTCTTGTAAACTTTACGGTGAAAGGCGCGGGGCGTTATCGTGCTG 2443 2 2153 .......................................................................................... 2242 A A N G D A T S L D L F H L P K M P A F S G Q L T A I V Q M Query_1 2444 CCGCTAATGGAGATGCAACTTCACTTGATTTATTCCACTTGCCGAAGATGCCCGCTTTCAGTGGTCAGCTGACAGCCATTGTTCAAATGA 2533 2 2243 .......................................................................................... 2332 T E Q P G E I I F E A K A K G V K S G K L V L R S V R E Query_1 2534 CCGAACAGCCCGGTGAAATTATTTTCGAGGCTAAGGCTAAAGGGGTGAAATCTGGTAAGCTTGTGCTGAGGTCTGTTAGAGAG 2616 2 2333 ................................................................................... 2415 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 13549702 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 4,885 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200027.107 (CMR200027.20-824.P(824).E502A.pMCSG68)
QRSEYLLEKNWKFMKGEAPEAMKPEFDDRKWETVTVPHDWAIFGPFDRSNDLQEVAVTQNFEKKASVKTGRTGGLPYVGIGWYRTRFDAPVNQQTTLVFDGAMSEARVYVNGQEACFWPFGYNSFHCDVTGLLNKDGKNNTLAVRLENKPQSSRWYPGAGLYRNVRVVSTDKVHVPVWGTQLTTPHVSDEYASVRLLTTIANDEERDIRIVTEIISPDGKVVATKDNTRKINHGQPFEQNFLVNAPCLWSPETPYLYKAVSKIYADGKQTDEYTTRFGIRSIEIIADKGFFLNGKHRKFQGVCNHHDLGPLGAAINVAALRRQLTMLKDMGCDAIRTAHNMPAPELVQLCDEMGFMMMLEPFDEWDIAKCENGYHRYFNEWAERDMINMLHQFRNNPCVVMWSIGNEVPTQCSPVGYKVASFLQDICHREDPTRPVTCGMDQVTCVLANGFAAMIDVPGFNYRAHRYLEAYELLPQNIVLGSETSSTVSSRGVYKFPVEKRGDAKYDDHQSSGYDLEHCAWSNVPDEDFALADDYDWTIGQFVWTGFDYLGEPSPYDTDAWPSHSSLFGIIDLASLPKDRYYLYRSLWNKNVNTLHILPHWTWPGREGENTPVFVYTNYPAAELFVNGKSYGKQHKLTAEESKAIQDKDTLALQRRYRLMWMDVPYEPGEVKVVAYDASGKPAEEKVVRTSGKPHHLEVIADRDQLTADGKDLAYITVRVVDKDGNLCPADNRLVNFTVKGAGRYRAAANGDATSLDLFHLPKMPAFSGQLTAIVQMTEQPGEIIFEAKAKGVKSGKLVLRSVRE
CAGCGCAGTGAGTATCTACTTGAAAAGAACTGGAAGTTCATGAAGGGGGAAGCTCCGGAAGCCATGAAGCCGGAATTTGACGACCGGAAGTGGGAAACCGTAACCGTGCCTCACGACTGGGCCATTTTTGGTCCCTTCGATCGCAGCAACGATTTGCAGGAAGTGGCGGTAACGCAGAACTTCGAGAAGAAAGCTTCCGTCAAGACCGGACGTACCGGTGGACTTCCTTATGTTGGCATCGGATGGTATCGTACTAGGTTCGATGCCCCCGTCAATCAACAGACGACACTTGTCTTTGATGGTGCCATGAGCGAAGCCCGTGTATATGTCAATGGACAAGAAGCATGCTTCTGGCCATTTGGTTATAATTCTTTCCATTGTGATGTCACCGGACTTTTGAATAAAGACGGTAAAAACAATACGCTTGCCGTGCGTTTGGAAAATAAACCACAATCTTCCCGTTGGTATCCTGGCGCAGGACTTTATCGCAATGTGCGTGTAGTGAGTACCGATAAAGTACATGTTCCTGTATGGGGTACTCAGCTGACTACTCCTCATGTTTCTGATGAGTATGCTTCAGTACGTCTGTTGACCACTATTGCCAATGATGAAGAAAGAGATATCCGTATCGTGACAGAGATAATCTCTCCCGATGGGAAAGTCGTTGCAACGAAGGATAATACCCGTAAGATTAATCATGGTCAGCCTTTTGAACAAAACTTCCTGGTGAATGCTCCTTGCTTGTGGTCGCCGGAGACACCTTATTTATATAAAGCTGTTTCTAAAATCTATGCCGATGGCAAGCAAACGGATGAATACACTACTCGTTTCGGCATCCGCAGCATAGAAATCATTGCCGACAAAGGATTTTTCCTGAACGGTAAGCATCGCAAGTTCCAGGGGGTGTGCAATCACCACGATCTTGGTCCGTTAGGCGCTGCCATCAATGTTGCTGCATTGCGCCGTCAACTTACGATGCTGAAAGATATGGGTTGTGATGCCATCCGCACCGCTCACAATATGCCGGCACCGGAGTTAGTGCAACTTTGTGATGAAATGGGTTTTATGATGATGCTGGAACCTTTCGACGAATGGGACATTGCCAAATGTGAGAATGGCTATCACCGTTATTTCAACGAGTGGGCAGAACGTGATATGATAAATATGTTGCATCAGTTCCGCAACAATCCTTGTGTCGTAATGTGGAGTATCGGTAATGAAGTTCCTACCCAATGTAGTCCCGTAGGCTATAAAGTCGCTTCTTTCTTGCAGGATATCTGTCATCGTGAAGATCCGACACGTCCTGTTACTTGCGGCATGGATCAGGTGACTTGTGTTCTTGCTAATGGTTTTGCCGCCATGATTGATGTGCCCGGTTTTAATTATCGCGCACACCGTTATCTGGAAGCTTATGAACTGTTGCCGCAGAATATAGTACTTGGTTCTGAAACATCCTCTACCGTTAGTTCTCGTGGCGTATATAAATTTCCTGTAGAGAAACGCGGGGATGCGAAGTACGATGATCACCAGTCTTCCGGATATGACTTGGAGCATTGTGCCTGGTCTAATGTTCCAGATGAAGATTTTGCTTTAGCGGATGATTATGACTGGACTATCGGTCAATTCGTTTGGACAGGATTCGATTATCTGGGTGAGCCTTCTCCTTATGATACGGATGCATGGCCAAGTCATAGCTCTTTGTTTGGTATCATTGACCTTGCCAGTTTGCCAAAAGACCGCTACTATCTGTACCGTAGTCTTTGGAATAAGAATGTGAATACACTCCATATACTTCCTCACTGGACATGGCCGGGTAGGGAAGGAGAGAATACTCCTGTCTTTGTTTACACAAACTATCCTGCTGCCGAACTTTTCGTTAATGGAAAAAGCTATGGTAAACAGCATAAACTGACAGCCGAAGAGAGTAAAGCTATTCAGGACAAAGATACACTTGCCCTCCAGCGTCGTTACCGCCTGATGTGGATGGACGTTCCTTATGAGCCGGGTGAAGTGAAAGTGGTGGCTTACGATGCTTCCGGCAAACCTGCTGAAGAAAAAGTAGTTCGTACTTCCGGCAAACCTCATCATCTGGAAGTCATTGCTGACCGTGACCAACTCACTGCCGATGGTAAAGATTTGGCATACATCACTGTTCGTGTGGTTGATAAAGACGGAAACCTTTGTCCTGCTGATAATCGTCTTGTAAACTTTACGGTGAAAGGCGCGGGGCGTTATCGTGCTGCCGCTAATGGAGATGCAACTTCACTTGATTTATTCCACTTGCCGAAGATGCCCGCTTTCAGTGGTCAGCTGACAGCCATTGTTCAAATGACCGAACAGCCCGGTGAAATTATTTTCGAGGCTAAGGCTAAAGGGGTGAAATCTGGTAAGCTTGTGCTGAGGTCTGTTAGAGAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,868 total letters Query= CMR200027.107 Length=2820 Score E Sequences producing significant alignments: (Bits) Value wt 4347 0.0 07F_F7_CMR200027.107_F07.seq 1442 0.0 I V S G * Q F P S R N N F V * L * E G D I H M H H H H H H S Query_1 40 ATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCT 129 2 1020 ........... 1010 S G V D L W S H P Q F E K G T E N L Y F Q S N A Q R S E Y L Query_1 130 TCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAGCGCAGTGAGTATCTA 219 2 1 .................. 18 L E K N W K F M K G E A P E A M K P E F D D R K W E T V T V Query_1 220 CTTGAAAAGAACTGGAAGTTCATGAAGGGGGAAGCTCCGGAAGCCATGAAGCCGGAATTTGACGACCGGAAGTGGGAAACCGTAACCGTG 309 2 19 .......................................................................................... 108 P H D W A I F G P F D R S N D L Q E V A V T Q N F E K K A S Query_1 310 CCTCACGACTGGGCCATTTTTGGTCCCTTCGATCGCAGCAACGATTTGCAGGAAGTGGCGGTAACGCAGAACTTCGAGAAGAAAGCTTCC 399 2 109 .......................................................................................... 198 V K T G R T G G L P Y V G I G W Y R T R F D A P V N Q Q T T Query_1 400 GTCAAGACCGGACGTACCGGTGGACTTCCTTATGTTGGCATCGGATGGTATCGTACTAGGTTCGATGCCCCCGTCAATCAACAGACGACA 489 2 199 .......................................................................................... 288 L V F D G A M S E A R V Y V N G Q E A C F W P F G Y N S F Query_1 490 CTTGTCTTTGATGGTGCCATGAGCGAAGCCCGTGTATATGTCAATGGACAAGAAGCATGCTTC-TGGCCATTTGGTTATAATTCTTTCCA 578 2 289 ...............................................................-.......................... 377 2 359 .....C..-............T..T..... 331 H C D V T G L L N K D G K N N T L A V R L E N K P Q S S R W Query_1 579 TTGTGATGTCACCGGACTTTTGAATAAAGACGGTAAAAACAATACGCTTGCCGTGCGTTTGGAAAATAAACCACAATCTTCCCGTTGGTA 668 2 378 .......................................................................................... 467 Y P G A G L Y R N V R V V S T D K V H V P V W G T Q L T T P Query_1 669 TCCTGGCGCAGGACTTTATCGCAATGTGCGTGTAGTGAGTACCGATAAAGTACATGTTCCTGTATGGGGTACTCAGCTGACTACTCCTCA 758 2 468 .......................................................................................... 557 H V S D E Y A S V R L L T T I A N D E E R D I R I V T E I I Query_1 759 TGTTTCTGATGAGTATGCTTCAGTACGTCTGTTGACCACTATTGCCAATGATGAAGAAAGAGATATCCGTATCGTGACAGAGATAATCTC 848 2 558 .......................................................................................... 647 S P D G K V V A T K D N T R K I N H G Q P F E Q N F L V N A Query_1 849 TCCCGATGGGAAAGTCGTTGCAACGAAGGATAATACCCGTAAGATTAATCATGGTCAGCCTTTTGAACAAAACTTCCTGGTGAATGCTCC 938 2 648 .......................................................................................... 737 2 673 ............ 662 P C L W S P E T P Y L Y K A V S K I Y A D G K Q T D E Y T T Query_1 939 TTGCTTGTGGTCGCCGGAGACACCTTATTTATATAAAGCTGTTTCTAAAATCTATGCCGATGGCAAGCAAACGGATGAATACACTACTCG 1028 2 738 .......................................................................................... 827 R F G I R S I E I I A D K G F F L N G K H R K F Q G V C N H Query_1 1029 TTTCGGCATCCGCAGCATAGAAATCATTGCCGACAAAGGATTTTTCCTGAACGGTAAGCATCGCAAGTTCCAGGGGGTGTGCAATCACCA 1118 2 828 .......................................................................................... 917 H D L G P L G A A I N V A A L R R Q L T M L K D M G C D A I Query_1 1119 CGATCTTGGTCCGTTAGGCGCTGCCATCAATGTTGCTGCATTGCGCCGTCAACTTACGATGCTGAAAGATATGGGTTGTGATGCCATCCG 1208 2 918 .......................................................................................... 1007 2 2024 ........... 2034 R T A H N M P A P E L V Q L C D E M G F M M M L E P F D E W Query_1 1209 CACCGCTCACAATATGCCGGCACCGGAGTTAGTGCAACTTTGTGATGAAATGGGTTTTATGATGATGCTGGAACCTTTCGACGAATGGGA 1298 2 1008 .......................................................................................... 1097 D I A K C E N G Y H R Y F N E W A E R D M I N M L H Q F R N Query_1 1299 CATTGCCAAATGTGAGAATGGCTATCACCGTTATTTCAACGAGTGGGCAGAACGTGATATGATAAATATGTTGCATCAG-TTCCGCAACA 1387 2 1098 ...............................................................................-.......... 1186 0 5 .....N.N...-........N......N..N.....................N.......... 66 N P C V V M W S I G N E V P T Q C S P V G Y K V A S F L Q Query_1 1388 ATCCTTGTGTCGTAATGTGGAGTATCGGTAATGAAGTTCCTACCCAATGTAGTCCCGT-AGGC-TATAAAGTCGCTTCTTTCTTGCAGGA 1475 2 1187 ..........................................................-....-.......................... 1274 0 67 ............N.............................................G....A.......................N.. 156 D I C H R E D P T R P V T C G M D Q V T C V L A N G F A A M Query_1 1476 TATCTGTCATCGTGAAGATCCGACACGTCCTGTTACTTGCGGCATGGATCAGGTGACTTGTGTTCTTGCTAATGGTTTTGCCGCCATGAT 1565 2 1275 .......................................................................................... 1364 0 157 .......................................................................................... 246 I D V P G F N Y R A H R Y L E A Y E L L P Q N I V L G S A T Query_1 1566 TGATGTGCCCGGTTTTAATTATCGCGCACACCGTTATCTGGAAGCTTATGAACTGTTGCCGCAGAATATAGTACTTGGTTCTGCTACATC 1655 2 1365 ...................................................................................AA..... 1454 0 247 .......................................................................A.................. 336 S S T V S S R G V Y K F P V E K R G D A K Y D D H Q S S G Y Query_1 1656 CTCTACCGTTAGTTCTCGTGGCGTATATAAATTTCCTGTAGAGAAACGCGGGGATGCGAAGTACGATGATCACCAGTCTTCCGGATATGA 1745 2 1455 .......................................................................................... 1544 0 337 .......................................................................................... 426 D L E H C A W S N V P D E D F A L A D D Y D W T I G Q F V W Query_1 1746 CTTGGAGCATTGTGCCTGGTCTAATGTTCCAGATGAAGATTTTGCTTTAGCGGATGATTATGACTGGACTATCGGTCAATTCGTTTGGAC 1835 2 1545 .......................................................................................... 1634 0 427 .................................................N........................................ 516 T G F D Y L G E P S P Y D T D A W P S H S S L F G I I D L A Query_1 1836 AGGATTCGATTATCTGGGTGAGCCTTCTCCTTATGATACGGATGCATGGCCAAGTCATAGCTCTTTGTTTGGTATCATTGACCTTGCCAG 1925 2 1635 .......................................................................................... 1724 0 517 .......................................................................................... 606 S L P K D R Y Y L Y R S L W N K N V N T L H I L P H W T W P Query_1 1926 TTTGCCAAAAGACCGCTACTATCTGTACCGTAGTCTTTGGAATAAGAATGTGAATACACTCCATATACTTCCTCACTGGACATGGCCGGG 2015 2 1725 .......................................................................................... 1814 0 607 .......................................................................................... 696 G R E G E N T P V F V Y T N Y P A A E L F V N G K S Y G K Q Query_1 2016 TAGGGAAGGAGAGAATACTCCTGTCTTTG-TTTACACAAACTATCCTGCTGCCGAACTT-TTCGTTAATGGAAAAAGCTATGGTAAACAG 2103 2 1815 .............................-.............................-.............................. 1902 0 697 .............................T.............................N.N.N.............N.N.......N.. 786 H K L T A E E S K A I Q D K D T L A L Q R R Y R L M W M Query_1 2104 CATAAAC-TGACAGCCGAAGAGAGTAAAGCTATTCAGGACAAA--GATACACTTG-CCCTCCAGCG-TCGTTA-CCGCCTGATGTGGATG 2187 2 1903 .......-...................................--..........-..........-......-................ 1986 0 787 .......T....G...N........N.................AGA.........C..........T..N...C........N.....N. 876 D V P Y E P G E V K V V A Y D A S G K P A E E K V Query_1 2188 GA--CGTTCCTT-ATGA--GCCGGGTGAAGTG--AAAGTGGT-GGCTT-ACGATGCTT-CCGGC--AAACCTGC-TGAAGAAA-AAGTAG 2263 2 1987 ..--........-....--.............--........-.....-.........-.....--........-........-...... 2062 2 970 ...-........ 980 0 877 ..ACNT......A.N..ACC....N.N.....AA........G.....N..N......N.N...CN.....G..NN.......N.....T 966 V R T S G K P H H L E V I A D R D Q L T A D G K D L A Y I Query_1 2264 TT-CGTACTT--CCGGCAAACCTCATCATC-TGGAAGTCATTGCTGACCGTGACCAACTCACTGCCGATGGTAAAGATTTGGCATACATC 2349 2 2063 ..-.......--..................-........................................................... 2148 0 967 ..N..N.NC.NC...C.......NC.N..NT.....N...... 1009 T V R V V D K D G N L C P A D N R L V N F T V K G A G R Y R Query_1 2350 ACTGTTCGTGTGGTTGATAAAGACGGAAACCTTTGTCCTGCTGATAATCGTCTTGTAAACTTTACGGTGAAAGGCGCGGGGCGTTATCGT 2439 2 2149 .......................................................................................... 2238 A A A N G D A T S L D L F H L P K M P A F S G Q L T A I V Q Query_1 2440 GCTGCCGCTAATGGAGATGCAACTTCACTTGATTTATTCCACTTGCCGAAGATGCCCGCTTTCAGTGGTCAGCTGACAGCCATTGTTCAA 2529 2 2239 .......................................................................................... 2328 M T E Q P G E I I F E A K A K G V K S G K L V L R S V R E Query_1 2530 ATGACCGAACAGCCCGGTGAAATTATTTTCGAGGCTAAGGCTAAAGGGGTGAAATCTGGTAAGCTTGTGCTGAGGTCTGTTAGAGAG 2616 2 2329 ....................................................................................... 2415 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 10699051 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 3,868 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200027.110 (CMR200027.20-824.P(824).Y481F.pMCSG68)
QRSEYLLEKNWKFMKGEAPEAMKPEFDDRKWETVTVPHDWAIFGPFDRSNDLQEVAVTQNFEKKASVKTGRTGGLPYVGIGWYRTRFDAPVNQQTTLVFDGAMSEARVYVNGQEACFWPFGYNSFHCDVTGLLNKDGKNNTLAVRLENKPQSSRWYPGAGLYRNVRVVSTDKVHVPVWGTQLTTPHVSDEYASVRLLTTIANDEERDIRIVTEIISPDGKVVATKDNTRKINHGQPFEQNFLVNAPCLWSPETPYLYKAVSKIYADGKQTDEYTTRFGIRSIEIIADKGFFLNGKHRKFQGVCNHHDLGPLGAAINVAALRRQLTMLKDMGCDAIRTAHNMPAPELVQLCDEMGFMMMLEPFDEWDIAKCENGYHRYFNEWAERDMINMLHQFRNNPCVVMWSIGNEVPTQCSPVGYKVASFLQDICHREDPTRPVTCGMDQVTCVLANGFAAMIDVPGFNYRAHRYLEAYELLPQNIVLGSETSSTVSSRGVYKFPVEKRGDAKYDDHQSSGYDLEHCAWSNVPDEDFALADDYDWTIGQFVWTGFDYLGEPSPYDTDAWPSHSSLFGIIDLASLPKDRYYLYRSLWNKNVNTLHILPHWTWPGREGENTPVFVYTNYPAAELFVNGKSYGKQHKLTAEESKAIQDKDTLALQRRYRLMWMDVPYEPGEVKVVAYDASGKPAEEKVVRTSGKPHHLEVIADRDQLTADGKDLAYITVRVVDKDGNLCPADNRLVNFTVKGAGRYRAAANGDATSLDLFHLPKMPAFSGQLTAIVQMTEQPGEIIFEAKAKGVKSGKLVLRSVRE
CAGCGCAGTGAGTATCTACTTGAAAAGAACTGGAAGTTCATGAAGGGGGAAGCTCCGGAAGCCATGAAGCCGGAATTTGACGACCGGAAGTGGGAAACCGTAACCGTGCCTCACGACTGGGCCATTTTTGGTCCCTTCGATCGCAGCAACGATTTGCAGGAAGTGGCGGTAACGCAGAACTTCGAGAAGAAAGCTTCCGTCAAGACCGGACGTACCGGTGGACTTCCTTATGTTGGCATCGGATGGTATCGTACTAGGTTCGATGCCCCCGTCAATCAACAGACGACACTTGTCTTTGATGGTGCCATGAGCGAAGCCCGTGTATATGTCAATGGACAAGAAGCATGCTTCTGGCCATTTGGTTATAATTCTTTCCATTGTGATGTCACCGGACTTTTGAATAAAGACGGTAAAAACAATACGCTTGCCGTGCGTTTGGAAAATAAACCACAATCTTCCCGTTGGTATCCTGGCGCAGGACTTTATCGCAATGTGCGTGTAGTGAGTACCGATAAAGTACATGTTCCTGTATGGGGTACTCAGCTGACTACTCCTCATGTTTCTGATGAGTATGCTTCAGTACGTCTGTTGACCACTATTGCCAATGATGAAGAAAGAGATATCCGTATCGTGACAGAGATAATCTCTCCCGATGGGAAAGTCGTTGCAACGAAGGATAATACCCGTAAGATTAATCATGGTCAGCCTTTTGAACAAAACTTCCTGGTGAATGCTCCTTGCTTGTGGTCGCCGGAGACACCTTATTTATATAAAGCTGTTTCTAAAATCTATGCCGATGGCAAGCAAACGGATGAATACACTACTCGTTTCGGCATCCGCAGCATAGAAATCATTGCCGACAAAGGATTTTTCCTGAACGGTAAGCATCGCAAGTTCCAGGGGGTGTGCAATCACCACGATCTTGGTCCGTTAGGCGCTGCCATCAATGTTGCTGCATTGCGCCGTCAACTTACGATGCTGAAAGATATGGGTTGTGATGCCATCCGCACCGCTCACAATATGCCGGCACCGGAGTTAGTGCAACTTTGTGATGAAATGGGTTTTATGATGATGCTGGAACCTTTCGACGAATGGGACATTGCCAAATGTGAGAATGGCTATCACCGTTATTTCAACGAGTGGGCAGAACGTGATATGATAAATATGTTGCATCAGTTCCGCAACAATCCTTGTGTCGTAATGTGGAGTATCGGTAATGAAGTTCCTACCCAATGTAGTCCCGTAGGCTATAAAGTCGCTTCTTTCTTGCAGGATATCTGTCATCGTGAAGATCCGACACGTCCTGTTACTTGCGGCATGGATCAGGTGACTTGTGTTCTTGCTAATGGTTTTGCCGCCATGATTGATGTGCCCGGTTTTAATTATCGCGCACACCGTTATCTGGAAGCTTATGAACTGTTGCCGCAGAATATAGTACTTGGTTCTGAAACATCCTCTACCGTTAGTTCTCGTGGCGTATATAAATTTCCTGTAGAGAAACGCGGGGATGCGAAGTACGATGATCACCAGTCTTCCGGATATGACTTGGAGCATTGTGCCTGGTCTAATGTTCCAGATGAAGATTTTGCTTTAGCGGATGATTATGACTGGACTATCGGTCAATTCGTTTGGACAGGATTCGATTATCTGGGTGAGCCTTCTCCTTATGATACGGATGCATGGCCAAGTCATAGCTCTTTGTTTGGTATCATTGACCTTGCCAGTTTGCCAAAAGACCGCTACTATCTGTACCGTAGTCTTTGGAATAAGAATGTGAATACACTCCATATACTTCCTCACTGGACATGGCCGGGTAGGGAAGGAGAGAATACTCCTGTCTTTGTTTACACAAACTATCCTGCTGCCGAACTTTTCGTTAATGGAAAAAGCTATGGTAAACAGCATAAACTGACAGCCGAAGAGAGTAAAGCTATTCAGGACAAAGATACACTTGCCCTCCAGCGTCGTTACCGCCTGATGTGGATGGACGTTCCTTATGAGCCGGGTGAAGTGAAAGTGGTGGCTTACGATGCTTCCGGCAAACCTGCTGAAGAAAAAGTAGTTCGTACTTCCGGCAAACCTCATCATCTGGAAGTCATTGCTGACCGTGACCAACTCACTGCCGATGGTAAAGATTTGGCATACATCACTGTTCGTGTGGTTGATAAAGACGGAAACCTTTGTCCTGCTGATAATCGTCTTGTAAACTTTACGGTGAAAGGCGCGGGGCGTTATCGTGCTGCCGCTAATGGAGATGCAACTTCACTTGATTTATTCCACTTGCCGAAGATGCCCGCTTTCAGTGGTCAGCTGACAGCCATTGTTCAAATGACCGAACAGCCCGGTGAAATTATTTTCGAGGCTAAGGCTAAAGGGGTGAAATCTGGTAAGCTTGTGCTGAGGTCTGTTAGAGAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,622 total letters Query= CMR200027.110 Length=2820 Score E Sequences producing significant alignments: (Bits) Value wt 4351 0.0 10G_G7_CMR200027.110_G07.seq 1876 0.0 I V S G * Q F P S R N N F V * L * E G D I H M H H H H H H S Query_1 40 ATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCT 129 2 1020 ........... 1010 1 519 ........... 529 S G V D L W S H P Q F E K G T E N L Y F Q S N A Q R S E Y L Query_1 130 TCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAGCGCAGTGAGTATCTA 219 2 1 .................. 18 L E K N W K F M K G E A P E A M K P E F D D R K W E T V T V Query_1 220 CTTGAAAAGAACTGGAAGTTCATGAAGGGGGAAGCTCCGGAAGCCATGAAGCCGGAATTTGACGACCGGAAGTGGGAAACCGTAACCGTG 309 2 19 .......................................................................................... 108 P H D W A I F G P F D R S N D L Q E V A V T Q N F E K K A S Query_1 310 CCTCACGACTGGGCCATTTTTGGTCCCTTCGATCGCAGCAACGATTTGCAGGAAGTGGCGGTAACGCAGAACTTCGAGAAGAAAGCTTCC 399 2 109 .......................................................................................... 198 V K T G R T G G L P Y V G I G W Y R T R F D A P V N Q Q T T Query_1 400 GTCAAGACCGGACGTACCGGTGGACTTCCTTATGTTGGCATCGGATGGTATCGTACTAGGTTCGATGCCCCCGTCAATCAACAGACGACA 489 2 199 .......................................................................................... 288 L V F D G A M S E A R V Y V N G Q E A C F W P F G Y N S F Query_1 490 CTTGTCTTTGATGGTGCCATGAGCGAAGCCCGTGTATATGTCAATGGACAAGAAGCATGCTTC-TGGCCATTTGGTTATAATTCTTTCCA 578 2 289 ...............................................................-.......................... 377 2 359 .....C..-............T..T..... 331 H C D V T G L L N K D G K N N T L A V R L E N K P Q S S R W Query_1 579 TTGTGATGTCACCGGACTTTTGAATAAAGACGGTAAAAACAATACGCTTGCCGTGCGTTTGGAAAATAAACCACAATCTTCCCGTTGGTA 668 2 378 .......................................................................................... 467 Y P G A G L Y R N V R V V S T D K V H V P V W G T Q L T T P Query_1 669 TCCTGGCGCAGGACTTTATCGCAATGTGCGTGTAGTGAGTACCGATAAAGTACATGTTCCTGTATGGGGTACTCAGCTGACTACTCCTCA 758 2 468 .......................................................................................... 557 1 1064 ..................................T................................................ 982 H V S D E Y A S V R L L T T I A N D E E R D I R I V T E I I Query_1 759 TGTTTCTGATGAGTATGCTTCAGTACGTCTGTTGACCACTATTGCCAATGATGAAGAAAGAGATATCCGTATCGTGACAGAGATAATCTC 848 2 558 .......................................................................................... 647 1 981 .......................................................................................... 892 S P D G K V V A T K D N T R K I N H G Q P F E Q N F L V N A Query_1 849 TCCCGATGGGAAAGTCGTTGCAACGAAGGATAATACCCGTAAGATTAATCATGGTCAGCCTTTTGAACAAAACTTCCTGGTGAATGCTCC 938 2 648 .......................................................................................... 737 2 673 ............ 662 1 891 .......................................................................................... 802 1 866 ............ 877 P C L W S P E T P Y L Y K A V S K I Y A D G K Q T D E Y T T Query_1 939 TTGCTTGTGGTCGCCGGAGACACCTTATTTATATAAAGCTGTTTCTAAAATCTATGCCGATGGCAAGCAAACGGATGAATACACTACTCG 1028 2 738 .......................................................................................... 827 1 801 .......................................................................................... 712 R F G I R S I E I I A D K G F F L N G K H R K F Q G V C N H Query_1 1029 TTTCGGCATCCGCAGCATAGAAATCATTGCCGACAAAGGATTTTTCCTGAACGGTAAGCATCGCAAGTTCCAGGGGGTGTGCAATCACCA 1118 2 828 .......................................................................................... 917 1 711 .......................................................................................... 622 H D L G P L G A A I N V A A L R R Q L T M L K D M G C D A I Query_1 1119 CGATCTTGGTCCGTTAGGCGCTGCCATCAATGTTGCTGCATTGCGCCGTCAACTTACGATGCTGAAAGATATGGGTTGTGATGCCATCCG 1208 2 918 .......................................................................................... 1007 2 2024 ........... 2034 1 621 .......................................................................................... 532 R T A H N M P A P E L V Q L C D E M G F M M M L E P F D E W Query_1 1209 CACCGCTCACAATATGCCGGCACCGGAGTTAGTGCAACTTTGTGATGAAATGGGTTTTATGATGATGCTGGAACCTTTCGACGAATGGGA 1298 2 1008 .......................................................................................... 1097 1 531 .......................................................................................... 442 D I A K C E N G Y H R Y F N E W A E R D M I N M L H Q F R N Query_1 1299 CATTGCCAAATGTGAGAATGGCTATCACCGTTATTTCAACGAGTGGGCAGAACGTGATATGATAAATATGTTGCATCAGTTCCGCAACAA 1388 2 1098 .......................................................................................... 1187 1 441 .......................................................................................... 352 N P C V V M W S I G N E V P T Q C S P V G Y K V A S F L Q D Query_1 1389 TCCTTGTGTCGTAATGTGGAGTATCGGTAATGAAGTTCCTACCCAATGTAGTCCCGTAGGCTATAAAGTCGCTTCTTTCTTGCAGGATAT 1478 2 1188 .......................................................................................... 1277 1 351 .......................................................................................... 262 I C H R E D P T R P V T C G M D Q V T C V L A N G F A A M I Query_1 1479 CTGTCATCGTGAAGATCCGACACGTCCTGTTACTTGCGGCATGGATCAGGTGACTTGTGTTCTTGCTAATGGTTTTGCCGCCATGATTGA 1568 2 1278 .......................................................................................... 1367 1 261 .......................................................................................... 172 D V P G F N F R A H R Y L E A Y E L L P Q N I V L G S E T S Query_1 1569 TGTGCCCGGTTTTAATTTTCGCGCACACCGTTATCTGGAAGCTTATGAACTGTTGCCGCAGAATATAGTACTTGGTTCTGAAACATCCTC 1658 2 1368 .................A........................................................................ 1457 1 171 .................A.............................................................C.G........ 82 S T V S S R G V Y K F P V E K R G D A K Y D D H Q S S G Y D Query_1 1659 TACCGTTAGTTCTCGTGGCGTATATAAATTTCCTGTAGAGAAACGCGGGGATGCGAAGTACGATGATCACCAGTCTTCCGGATATGACTT 1748 2 1458 .......................................................................................... 1547 1 81 .................................................................NN....NT... 6 L E H C A W S N V P D E D F A L A D D Y D W T I G Q F V W T Query_1 1749 GGAGCATTGTGCCTGGTCTAATGTTCCAGATGAAGATTTTGCTTTAGCGGATGATTATGACTGGACTATCGGTCAATTCGTTTGGACAGG 1838 2 1548 .......................................................................................... 1637 G F D Y L G E P S P Y D T D A W P S H S S L F G I I D L A S Query_1 1839 ATTCGATTATCTGGGTGAGCCTTCTCCTTATGATACGGATGCATGGCCAAGTCATAGCTCTTTGTTTGGTATCATTGACCTTGCCAGTTT 1928 2 1638 .......................................................................................... 1727 L P K D R Y Y L Y R S L W N K N V N T L H I L P H W T W P G Query_1 1929 GCCAAAAGACCGCTACTATCTGTACCGTAGTCTTTGGAATAAGAATGTGAATACACTCCATATACTTCCTCACTGGACATGGCCGGGTAG 2018 2 1728 .......................................................................................... 1817 R E G E N T P V F V Y T N Y P A A E L F V N G K S Y G K Q H Query_1 2019 GGAAGGAGAGAATACTCCTGTCTTTGTTTACACAAACTATCCTGCTGCCGAACTTTTCGTTAATGGAAAAAGCTATGGTAAACAGCATAA 2108 2 1818 .......................................................................................... 1907 K L T A E E S K A I Q D K D T L A L Q R R Y R L M W M D V P Query_1 2109 ACTGACAGCCGAAGAGAGTAAAGCTATTCAGGACAAAGATACACTTGCCCTCCAGCGTCGTTACCGCCTGATGTGGATGGACGTTCCTTA 2198 2 1908 .......................................................................................... 1997 Y E P G E V K V V A Y D A S G K P A E E K V V R T S G K P H Query_1 2199 TGAGCCGGGTGAAGTGAAAGTGGTGGCTTACGATGCTTCCGGCAAACCTGCTGAAGAAAAAGTAGTTCGTACTTCCGGCAAACCTCATCA 2288 2 1998 .......................................................................................... 2087 2 970 ........... 980 1 569 ........... 559 H L E V I A D R D Q L T A D G K D L A Y I T V R V V D K D G Query_1 2289 TCTGGAAGTCATTGCTGACCGTGACCAACTCACTGCCGATGGTAAAGATTTGGCATACATCACTGTTCGTGTGGTTGATAAAGACGGAAA 2378 2 2088 .......................................................................................... 2177 1 1126 ............T... 1111 N L C P A D N R L V N F T V K G A G R Y R A A A N G D A T S Query_1 2379 CCTTTGTCCTGCTGATAATCGTCTTGTAAACTTTACGGTGAAAGGCGCGGGGCGTTATCGTGCTGCCGCTAATGGAGATGCAACTTCACT 2468 2 2178 .......................................................................................... 2267 L D L F H L P K M P A F S G Q L T A I V Q M T E Q P G E I I Query_1 2469 TGATTTATTCCACTTGCCGAAGATGCCCGCTTTCAGTGGTCAGCTGACAGCCATTGTTCAAATGACCGAACAGCCCGGTGAAATTATTTT 2558 2 2268 .......................................................................................... 2357 F E A K A K G V K S G K L V L R S V R E Query_1 2559 CGAGGCTAAGGCTAAAGGGGTGAAATCTGGTAAGCTTGTGCTGAGGTCTGTTAGAGAG 2616 2 2358 .......................................................... 2415 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 10009513 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 3,622 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200027.114 (CMR200027.20-824.P(824).E502Q.pMCSG68)
QRSEYLLEKNWKFMKGEAPEAMKPEFDDRKWETVTVPHDWAIFGPFDRSNDLQEVAVTQNFEKKASVKTGRTGGLPYVGIGWYRTRFDAPVNQQTTLVFDGAMSEARVYVNGQEACFWPFGYNSFHCDVTGLLNKDGKNNTLAVRLENKPQSSRWYPGAGLYRNVRVVSTDKVHVPVWGTQLTTPHVSDEYASVRLLTTIANDEERDIRIVTEIISPDGKVVATKDNTRKINHGQPFEQNFLVNAPCLWSPETPYLYKAVSKIYADGKQTDEYTTRFGIRSIEIIADKGFFLNGKHRKFQGVCNHHDLGPLGAAINVAALRRQLTMLKDMGCDAIRTAHNMPAPELVQLCDEMGFMMMLEPFDEWDIAKCENGYHRYFNEWAERDMINMLHQFRNNPCVVMWSIGNEVPTQCSPVGYKVASFLQDICHREDPTRPVTCGMDQVTCVLANGFAAMIDVPGFNYRAHRYLEAYELLPQNIVLGSETSSTVSSRGVYKFPVEKRGDAKYDDHQSSGYDLEHCAWSNVPDEDFALADDYDWTIGQFVWTGFDYLGEPSPYDTDAWPSHSSLFGIIDLASLPKDRYYLYRSLWNKNVNTLHILPHWTWPGREGENTPVFVYTNYPAAELFVNGKSYGKQHKLTAEESKAIQDKDTLALQRRYRLMWMDVPYEPGEVKVVAYDASGKPAEEKVVRTSGKPHHLEVIADRDQLTADGKDLAYITVRVVDKDGNLCPADNRLVNFTVKGAGRYRAAANGDATSLDLFHLPKMPAFSGQLTAIVQMTEQPGEIIFEAKAKGVKSGKLVLRSVRE
CAGCGCAGTGAGTATCTACTTGAAAAGAACTGGAAGTTCATGAAGGGGGAAGCTCCGGAAGCCATGAAGCCGGAATTTGACGACCGGAAGTGGGAAACCGTAACCGTGCCTCACGACTGGGCCATTTTTGGTCCCTTCGATCGCAGCAACGATTTGCAGGAAGTGGCGGTAACGCAGAACTTCGAGAAGAAAGCTTCCGTCAAGACCGGACGTACCGGTGGACTTCCTTATGTTGGCATCGGATGGTATCGTACTAGGTTCGATGCCCCCGTCAATCAACAGACGACACTTGTCTTTGATGGTGCCATGAGCGAAGCCCGTGTATATGTCAATGGACAAGAAGCATGCTTCTGGCCATTTGGTTATAATTCTTTCCATTGTGATGTCACCGGACTTTTGAATAAAGACGGTAAAAACAATACGCTTGCCGTGCGTTTGGAAAATAAACCACAATCTTCCCGTTGGTATCCTGGCGCAGGACTTTATCGCAATGTGCGTGTAGTGAGTACCGATAAAGTACATGTTCCTGTATGGGGTACTCAGCTGACTACTCCTCATGTTTCTGATGAGTATGCTTCAGTACGTCTGTTGACCACTATTGCCAATGATGAAGAAAGAGATATCCGTATCGTGACAGAGATAATCTCTCCCGATGGGAAAGTCGTTGCAACGAAGGATAATACCCGTAAGATTAATCATGGTCAGCCTTTTGAACAAAACTTCCTGGTGAATGCTCCTTGCTTGTGGTCGCCGGAGACACCTTATTTATATAAAGCTGTTTCTAAAATCTATGCCGATGGCAAGCAAACGGATGAATACACTACTCGTTTCGGCATCCGCAGCATAGAAATCATTGCCGACAAAGGATTTTTCCTGAACGGTAAGCATCGCAAGTTCCAGGGGGTGTGCAATCACCACGATCTTGGTCCGTTAGGCGCTGCCATCAATGTTGCTGCATTGCGCCGTCAACTTACGATGCTGAAAGATATGGGTTGTGATGCCATCCGCACCGCTCACAATATGCCGGCACCGGAGTTAGTGCAACTTTGTGATGAAATGGGTTTTATGATGATGCTGGAACCTTTCGACGAATGGGACATTGCCAAATGTGAGAATGGCTATCACCGTTATTTCAACGAGTGGGCAGAACGTGATATGATAAATATGTTGCATCAGTTCCGCAACAATCCTTGTGTCGTAATGTGGAGTATCGGTAATGAAGTTCCTACCCAATGTAGTCCCGTAGGCTATAAAGTCGCTTCTTTCTTGCAGGATATCTGTCATCGTGAAGATCCGACACGTCCTGTTACTTGCGGCATGGATCAGGTGACTTGTGTTCTTGCTAATGGTTTTGCCGCCATGATTGATGTGCCCGGTTTTAATTATCGCGCACACCGTTATCTGGAAGCTTATGAACTGTTGCCGCAGAATATAGTACTTGGTTCTGAAACATCCTCTACCGTTAGTTCTCGTGGCGTATATAAATTTCCTGTAGAGAAACGCGGGGATGCGAAGTACGATGATCACCAGTCTTCCGGATATGACTTGGAGCATTGTGCCTGGTCTAATGTTCCAGATGAAGATTTTGCTTTAGCGGATGATTATGACTGGACTATCGGTCAATTCGTTTGGACAGGATTCGATTATCTGGGTGAGCCTTCTCCTTATGATACGGATGCATGGCCAAGTCATAGCTCTTTGTTTGGTATCATTGACCTTGCCAGTTTGCCAAAAGACCGCTACTATCTGTACCGTAGTCTTTGGAATAAGAATGTGAATACACTCCATATACTTCCTCACTGGACATGGCCGGGTAGGGAAGGAGAGAATACTCCTGTCTTTGTTTACACAAACTATCCTGCTGCCGAACTTTTCGTTAATGGAAAAAGCTATGGTAAACAGCATAAACTGACAGCCGAAGAGAGTAAAGCTATTCAGGACAAAGATACACTTGCCCTCCAGCGTCGTTACCGCCTGATGTGGATGGACGTTCCTTATGAGCCGGGTGAAGTGAAAGTGGTGGCTTACGATGCTTCCGGCAAACCTGCTGAAGAAAAAGTAGTTCGTACTTCCGGCAAACCTCATCATCTGGAAGTCATTGCTGACCGTGACCAACTCACTGCCGATGGTAAAGATTTGGCATACATCACTGTTCGTGTGGTTGATAAAGACGGAAACCTTTGTCCTGCTGATAATCGTCTTGTAAACTTTACGGTGAAAGGCGCGGGGCGTTATCGTGCTGCCGCTAATGGAGATGCAACTTCACTTGATTTATTCCACTTGCCGAAGATGCCCGCTTTCAGTGGTCAGCTGACAGCCATTGTTCAAATGACCGAACAGCCCGGTGAAATTATTTTCGAGGCTAAGGCTAAAGGGGTGAAATCTGGTAAGCTTGTGCTGAGGTCTGTTAGAGAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,899 total letters Query= CMR200027.114 Length=2820 Score E Sequences producing significant alignments: (Bits) Value wt 4347 0.0 07H_H7_CMR200027.114_H07.seq 1898 0.0 10H_H7_CMR200027.114_H07.seq 1887 0.0 I V S G * Q F P S R N N F V * L * E G D I H M H H H H H H S Query_1 40 ATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCT 129 2 1020 ........... 1010 1 522 ........... 532 S G V D L W S H P Q F E K G T E N L Y F Q S N A Q R S E Y L Query_1 130 TCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAGCGCAGTGAGTATCTA 219 2 1 .................. 18 L E K N W K F M K G E A P E A M K P E F D D R K W E T V T V Query_1 220 CTTGAAAAGAACTGGAAGTTCATGAAGGGGGAAGCTCCGGAAGCCATGAAGCCGGAATTTGACGACCGGAAGTGGGAAACCGTAACCGTG 309 2 19 .......................................................................................... 108 P H D W A I F G P F D R S N D L Q E V A V T Q N F E K K A S Query_1 310 CCTCACGACTGGGCCATTTTTGGTCCCTTCGATCGCAGCAACGATTTGCAGGAAGTGGCGGTAACGCAGAACTTCGAGAAGAAAGCTTCC 399 2 109 .......................................................................................... 198 V K T G R T G G L P Y V G I G W Y R T R F D A P V N Q Q T T Query_1 400 GTCAAGACCGGACGTACCGGTGGACTTCCTTATGTTGGCATCGGATGGTATCGTACTAGGTTCGATGCCCCCGTCAATCAACAGACGACA 489 2 199 .......................................................................................... 288 L V F D G A M S E A R V Y V N G Q E A C F W P F G Y N S F Query_1 490 CTTGTCTTTGATGGTGCCATGAGCGAAGCCCGTGTATATGTCAATGGACAAGAAGCATGCTTC-TGGCCATTTGGTTATAATTCTTTCCA 578 2 289 ...............................................................-.......................... 377 2 359 .....C..-............T..T..... 331 1 1171 ...N...N...AN 1159 H C D V T G L L N K D G K N N T L A V R L E N K P Q S S R W Query_1 579 TTGT-GA-TGTCACCGGACTTTTGAATAAAGACGGTAAAAACAATACGCTTGCCGTGCGTTTGGAAAATAAACCACAATCTTCCCGTTGG 666 2 378 ....-..-.................................................................................. 465 1 1158 ...NA..N...NN..C.....--......-.....N.......-......NNN--...N..-..N....N...NCN........N--... 1078 Y P G A G L Y R N V R V V S T D K V H V P V W G T Q L T T Query_1 667 TATCCTGGCGC-AGGACTTTATCGCAATGTGCGTGTAGTGAGTACCGATAAAGTACATGTTCCTGTATGGGGTACTCAGCTGACTACTCC 755 2 466 ...........-.............................................................................. 554 1 1077 ........N..C.......-........................T............................................. 989 P H V S D E Y A S V R L L T T I A N D E E R D I R I V T E I Query_1 756 TCATGTTTCTGATGAGTATGCTTCAGTACGTCTGTTGACCACTATTGCCAATGATGAAGAAAGAGATATCCGTATCGTGACAGAGATAAT 845 2 555 .......................................................................................... 644 1 988 ......................C................................................................... 899 I S P D G K V V A T K D N T R K I N H G Q P F E Q N F L V N Query_1 846 CTCTCCCGATGGGAAAGTCGTTGCAACGAAGGATAATACCCGTAAGATTAATCATGGTCAGCCTTTTGAACAAAACTTCCTGGTGAATGC 935 2 645 .......................................................................................... 734 2 673 ............ 662 1 898 .......................................................................................... 809 1 870 ............ 881 A P C L W S P E T P Y L Y K A V S K I Y A D G K Q T D E Y T Query_1 936 TCCTTGCTTGTGGTCGCCGGAGACACCTTATTTATATAAAGCTGTTTCTAAAA-TCTATGCCGATGGCAAGCAAACGGATGAATACACTA 1024 2 735 .....................................................-.................................... 823 1 808 .....................................................A.................................... 719 T R F G I R S I E I I A D K G F F L N G K H R K F Q G V C N Query_1 1025 CTCGTTTCGGCATCCGCAGCATAGAAATCATTGCCGACAAAGGATTTTTCCTGAACGGTAAGCATCGCAAGTTCCAGGGGGTGTGCAATC 1114 2 824 .......................................................................................... 913 1 718 .......................................................................................... 629 H H D L G P L G A A I N V A A L R R Q L T M L K D M G C D A Query_1 1115 ACCACGATCTTGGTCCGTTAGGCGCTGCCATCAATGTTGCTGCATTGCGCCGTCAACTTACGATGCTGAAAGATATGGGTTGTGATGCCA 1204 2 914 .......................................................................................... 1003 1 628 .......................................................................................... 539 I R T A H N M P A P E L V Q L C D E M G F M M M L E P F D E Query_1 1205 TCCGCACCGCTCACAATATGCCGGCACCGGAGTTAGTGCAACTTTGTGATGAAATGGGTTTTATGATGATGCTGGAACCTTTCGACGAAT 1294 2 1004 .......................................................................................... 1093 1 538 .......................................................................................... 449 W D I A K C E N G Y H R Y F N E W A E R D M I N M L H Q F R Query_1 1295 GGGACATTGCCAAATGTGAGAATGGCTATCACCGTTATTTCAACGAGTGGGCAGAACGTGATATGATAAATATGTTGCATCAGTTCCGCA 1384 2 1094 .......................................................................................... 1183 0 14 ......N-...........-............................................... 78 1 448 .......................................................................................... 359 N N P C V V M W S I G N E V P T Q C S P V G Y K V A S F L Q Query_1 1385 ACAATCCTTGTGTCGTAATGTGGAGTATCGGTAATGAAGTTCCTACCCAATGTAGTCCCGTAGGCTATAAAGTCGCTTCTTTCTTGCAGG 1474 2 1184 .......................................................................................... 1273 0 79 .......................................................................................... 168 1 358 .......................................................................................... 269 D I C H R E D P T R P V T C G M D Q V T C V L A N G F A A M Query_1 1475 ATATCTGTCATCGTGAAGATCCGACACGTCCTGTTACTTGCGGCATGGATCAGGTGACTTGTGTTCTTGCTAATGGTTTTGCCGCCATGA 1564 2 1274 .......................................................................................... 1363 0 169 .......................................................................................... 258 1 268 .......................................................................................... 179 I D V P G F N Y R A H R Y L E A Y E L L P Q N I V L G S Q T Query_1 1565 TTGATGTGCCCGGTTTTAATTATCGCGCACACCGTTATCTGGAAGCTTATGAACTGTTGCCGCAGAATATAGTACTTGGTTCTCAGACAT 1654 2 1364 ...................................................................................G.A.... 1453 0 259 .......................................................................................... 348 1 178 ....................GC.............................................................G.A.... 89 S S T V S S R G V Y K F P V E K R G D A K Y D D H Q S S G Y Query_1 1655 CCTCTACCGTTAGTTCTCGTGGCGTATATAAATTTCCTGTAGAGAAACGCGGGGATGCGAAGTACGATGATCACCAGTCTTCCGGATATG 1744 2 1454 .......................................................................................... 1543 0 349 .......................................................................................... 438 1 88 ................................................................. 24 D L E H C A W S N V P D E D F A L A D D Y D W T I G Q F V W Query_1 1745 ACTTGGAGCATTGTGCCTGGTCTAATGTTCCAGATGAAGATTTTGCTTTAGCGGATGATTATGACTGGACTATCGGTCAATTCGTTTGGA 1834 2 1544 .......................................................................................... 1633 0 439 .......................................................................................... 528 T G F D Y L G E P S P Y D T D A W P S H S S L F G I I D L A Query_1 1835 CAGGATTCGATTATCTGGGTGAGCCTTCTCCTTATGATACGGATGCATGGCCAAGTCATAGCTCTTTGTTTGGTATCATTGACCTTGCCA 1924 2 1634 .......................................................................................... 1723 0 529 .......................................................................................... 618 S L P K D R Y Y L Y R S L W N K N V N T L H I L P H W T W P Query_1 1925 GTTTGCCAAAAGACCGCTACTATCTGTACCGTAGTCTTTGGAATAAGAATGTGAATACACTCCATATACTTCCTCACTGGACATGGCCGG 2014 2 1724 .......................................................................................... 1813 0 619 .......................................................................................... 708 G R E G E N T P V F V Y T N Y P A A E L F V N G K S Y G K Q Query_1 2015 GTAGGGAAGGAGAGAATACTCCTGTCTTTGTTTACACAAACTATCCTGCTGCCGAACTTTTCGTTAATGGAAAAAGCTATGGTAAACAGC 2104 2 1814 .......................................................................................... 1903 0 709 .......................................................................................... 798 H K L T A E E S K A I Q D K D T L A L Q R R Y R L M W M D V Query_1 2105 ATAAACTGACAGCCGAAGAGAGTAAAGCTATTCAGGACAAAGATACACTTGCCCTCCAGCGTCGTTACCGCCTGATGTGGATGGACGTTC 2194 2 1904 .......................................................................................... 1993 0 799 .......................................................................................... 888 P Y E P G E V K V V A Y D A S G K P A E E K V V R T S G K P Query_1 2195 CTTATGAGCCGGGTGAAGTGAAAGTGGTGGCTTACGATGCTTCCGGCAAACCTGCTGAAGAAAAAGTAGTTCGTACTTCCGGCAAACCTC 2284 2 1994 .......................................................................................... 2083 2 970 ........... 980 0 889 ......................................................................................-... 977 1 572 ........... 562 H H L E V I A D R D Q L T A D G K D L A Y I T V R V V D K D Query_1 2285 ATCATCTGGAAGTCATTGCTGACCGTGACCAACTCACTGCCGATGGTAAAGATTTGGCATACATCACTGTTCGTGTGGTTGATAAAGACG 2374 2 2084 .......................................................................................... 2173 0 978 ..............................................-N.N............N..........N....N....-...... 1065 G N L C P A D N R L V N F T V K G A G R Y R A A A N G D A T Query_1 2375 GAAACCTTTGTCCTGCTGATAATCGTCTTGTAAACTTTACGGTGAAAGGCGCGGGGCGTTATC-GTGCTGCCGCTAATGGAGATGCAACT 2463 2 2174 ...............................................................-.......................... 2262 0 1066 N..--.N.............-......................NN.N.--NNNN.NNN.AN..NN.N...-....-.............. 1148 S L D L F H L P K M P A F S G Q L T A I V Q M T E Q P G E I Query_1 2464 TCACTTGATTTATTCCACTTGCCGAAGATGCCCGCTTTCAGTGGTCAGCTGACAGCCATTGTTCAAATGACCGAACAGCCCGGTGAAATT 2553 2 2263 .......................................................................................... 2352 0 1149 ..N.-..... 1157 I F E A K A K G V K S G K L V L R S V R E Query_1 2554 ATTTTCGAGGCTAAGGCTAAAGGGGTGAAATCTGGTAAGCTTGTGCTGAGGTCTGTTAGAGAG 2616 2 2353 ............................................................... 2415 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 13588944 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 4,899 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200027.115 (CMR200027.20-824.P(824).Y481A.pMCSG68)
QRSEYLLEKNWKFMKGEAPEAMKPEFDDRKWETVTVPHDWAIFGPFDRSNDLQEVAVTQNFEKKASVKTGRTGGLPYVGIGWYRTRFDAPVNQQTTLVFDGAMSEARVYVNGQEACFWPFGYNSFHCDVTGLLNKDGKNNTLAVRLENKPQSSRWYPGAGLYRNVRVVSTDKVHVPVWGTQLTTPHVSDEYASVRLLTTIANDEERDIRIVTEIISPDGKVVATKDNTRKINHGQPFEQNFLVNAPCLWSPETPYLYKAVSKIYADGKQTDEYTTRFGIRSIEIIADKGFFLNGKHRKFQGVCNHHDLGPLGAAINVAALRRQLTMLKDMGCDAIRTAHNMPAPELVQLCDEMGFMMMLEPFDEWDIAKCENGYHRYFNEWAERDMINMLHQFRNNPCVVMWSIGNEVPTQCSPVGYKVASFLQDICHREDPTRPVTCGMDQVTCVLANGFAAMIDVPGFNYRAHRYLEAYELLPQNIVLGSETSSTVSSRGVYKFPVEKRGDAKYDDHQSSGYDLEHCAWSNVPDEDFALADDYDWTIGQFVWTGFDYLGEPSPYDTDAWPSHSSLFGIIDLASLPKDRYYLYRSLWNKNVNTLHILPHWTWPGREGENTPVFVYTNYPAAELFVNGKSYGKQHKLTAEESKAIQDKDTLALQRRYRLMWMDVPYEPGEVKVVAYDASGKPAEEKVVRTSGKPHHLEVIADRDQLTADGKDLAYITVRVVDKDGNLCPADNRLVNFTVKGAGRYRAAANGDATSLDLFHLPKMPAFSGQLTAIVQMTEQPGEIIFEAKAKGVKSGKLVLRSVRE
CAGCGCAGTGAGTATCTACTTGAAAAGAACTGGAAGTTCATGAAGGGGGAAGCTCCGGAAGCCATGAAGCCGGAATTTGACGACCGGAAGTGGGAAACCGTAACCGTGCCTCACGACTGGGCCATTTTTGGTCCCTTCGATCGCAGCAACGATTTGCAGGAAGTGGCGGTAACGCAGAACTTCGAGAAGAAAGCTTCCGTCAAGACCGGACGTACCGGTGGACTTCCTTATGTTGGCATCGGATGGTATCGTACTAGGTTCGATGCCCCCGTCAATCAACAGACGACACTTGTCTTTGATGGTGCCATGAGCGAAGCCCGTGTATATGTCAATGGACAAGAAGCATGCTTCTGGCCATTTGGTTATAATTCTTTCCATTGTGATGTCACCGGACTTTTGAATAAAGACGGTAAAAACAATACGCTTGCCGTGCGTTTGGAAAATAAACCACAATCTTCCCGTTGGTATCCTGGCGCAGGACTTTATCGCAATGTGCGTGTAGTGAGTACCGATAAAGTACATGTTCCTGTATGGGGTACTCAGCTGACTACTCCTCATGTTTCTGATGAGTATGCTTCAGTACGTCTGTTGACCACTATTGCCAATGATGAAGAAAGAGATATCCGTATCGTGACAGAGATAATCTCTCCCGATGGGAAAGTCGTTGCAACGAAGGATAATACCCGTAAGATTAATCATGGTCAGCCTTTTGAACAAAACTTCCTGGTGAATGCTCCTTGCTTGTGGTCGCCGGAGACACCTTATTTATATAAAGCTGTTTCTAAAATCTATGCCGATGGCAAGCAAACGGATGAATACACTACTCGTTTCGGCATCCGCAGCATAGAAATCATTGCCGACAAAGGATTTTTCCTGAACGGTAAGCATCGCAAGTTCCAGGGGGTGTGCAATCACCACGATCTTGGTCCGTTAGGCGCTGCCATCAATGTTGCTGCATTGCGCCGTCAACTTACGATGCTGAAAGATATGGGTTGTGATGCCATCCGCACCGCTCACAATATGCCGGCACCGGAGTTAGTGCAACTTTGTGATGAAATGGGTTTTATGATGATGCTGGAACCTTTCGACGAATGGGACATTGCCAAATGTGAGAATGGCTATCACCGTTATTTCAACGAGTGGGCAGAACGTGATATGATAAATATGTTGCATCAGTTCCGCAACAATCCTTGTGTCGTAATGTGGAGTATCGGTAATGAAGTTCCTACCCAATGTAGTCCCGTAGGCTATAAAGTCGCTTCTTTCTTGCAGGATATCTGTCATCGTGAAGATCCGACACGTCCTGTTACTTGCGGCATGGATCAGGTGACTTGTGTTCTTGCTAATGGTTTTGCCGCCATGATTGATGTGCCCGGTTTTAATTATCGCGCACACCGTTATCTGGAAGCTTATGAACTGTTGCCGCAGAATATAGTACTTGGTTCTGAAACATCCTCTACCGTTAGTTCTCGTGGCGTATATAAATTTCCTGTAGAGAAACGCGGGGATGCGAAGTACGATGATCACCAGTCTTCCGGATATGACTTGGAGCATTGTGCCTGGTCTAATGTTCCAGATGAAGATTTTGCTTTAGCGGATGATTATGACTGGACTATCGGTCAATTCGTTTGGACAGGATTCGATTATCTGGGTGAGCCTTCTCCTTATGATACGGATGCATGGCCAAGTCATAGCTCTTTGTTTGGTATCATTGACCTTGCCAGTTTGCCAAAAGACCGCTACTATCTGTACCGTAGTCTTTGGAATAAGAATGTGAATACACTCCATATACTTCCTCACTGGACATGGCCGGGTAGGGAAGGAGAGAATACTCCTGTCTTTGTTTACACAAACTATCCTGCTGCCGAACTTTTCGTTAATGGAAAAAGCTATGGTAAACAGCATAAACTGACAGCCGAAGAGAGTAAAGCTATTCAGGACAAAGATACACTTGCCCTCCAGCGTCGTTACCGCCTGATGTGGATGGACGTTCCTTATGAGCCGGGTGAAGTGAAAGTGGTGGCTTACGATGCTTCCGGCAAACCTGCTGAAGAAAAAGTAGTTCGTACTTCCGGCAAACCTCATCATCTGGAAGTCATTGCTGACCGTGACCAACTCACTGCCGATGGTAAAGATTTGGCATACATCACTGTTCGTGTGGTTGATAAAGACGGAAACCTTTGTCCTGCTGATAATCGTCTTGTAAACTTTACGGTGAAAGGCGCGGGGCGTTATCGTGCTGCCGCTAATGGAGATGCAACTTCACTTGATTTATTCCACTTGCCGAAGATGCCCGCTTTCAGTGGTCAGCTGACAGCCATTGTTCAAATGACCGAACAGCCCGGTGAAATTATTTTCGAGGCTAAGGCTAAAGGGGTGAAATCTGGTAAGCTTGTGCTGAGGTCTGTTAGAGAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,121 total letters Query= CMR200027.115 Length=2820 Score E Sequences producing significant alignments: (Bits) Value wt 4347 0.0 08A_A8_CMR200027.115_A08.seq 1837 0.0 I V S G * Q F P S R N N F V * L * E G D I H M H H H H H H S Query_1 40 ATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCT 129 2 1020 ........... 1010 S G V D L W S H P Q F E K G T E N L Y F Q S N A Q R S E Y L Query_1 130 TCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAGCGCAGTGAGTATCTA 219 2 1 .................. 18 L E K N W K F M K G E A P E A M K P E F D D R K W E T V T V Query_1 220 CTTGAAAAGAACTGGAAGTTCATGAAGGGGGAAGCTCCGGAAGCCATGAAGCCGGAATTTGACGACCGGAAGTGGGAAACCGTAACCGTG 309 2 19 .......................................................................................... 108 P H D W A I F G P F D R S N D L Q E V A V T Q N F E K K A S Query_1 310 CCTCACGACTGGGCCATTTTTGGTCCCTTCGATCGCAGCAACGATTTGCAGGAAGTGGCGGTAACGCAGAACTTCGAGAAGAAAGCTTCC 399 2 109 .......................................................................................... 198 V K T G R T G G L P Y V G I G W Y R T R F D A P V N Q Q T T Query_1 400 GTCAAGACCGGACGTACCGGTGGACTTCCTTATGTTGGCATCGGATGGTATCGTACTAGGTTCGATGCCCCCGTCAATCAACAGACGACA 489 2 199 .......................................................................................... 288 L V F D G A M S E A R V Y V N G Q E A C F W P F G Y N S F Query_1 490 CTTGTCTTTGATGGTGCCATGAGCGAAGCCCGTGTATATGTCAATGGACAAGAAGCATGCTTC-TGGCCATTTGGTTATAATTCTTTCCA 578 2 289 ...............................................................-.......................... 377 2 359 .....C..-............T..T..... 331 H C D V T G L L N K D G K N N T L A V R L E N K P Q S S R W Query_1 579 TTGTGATGTCACCGGACTTTTGAATAAAGACGGTAAAAACAATACGCTTGCCGTGCGTTTGGAAAATAAACCACAATCTTCCCGTTGGTA 668 2 378 .......................................................................................... 467 Y P G A G L Y R N V R V V S T D K V H V P V W G T Q L T T P Query_1 669 TCCTGGCGCAGGACTTTATCGCAATGTGCGTGTAGTGAGTACCGATAAAGTACATGTTCCTGTATGGGGTACTCAGCTGACTACTCCTCA 758 2 468 .......................................................................................... 557 H V S D E Y A S V R L L T T I A N D E E R D I R I V T E I I Query_1 759 TGTTTCTGATGAGTATGCTTCAGTACGTCTGTTGACCACTATTGCCAATGATGAAGAAAGAGATATCCGTATCGTGACAGAGATAATCTC 848 2 558 .......................................................................................... 647 S P D G K V V A T K D N T R K I N H G Q P F E Q N F L V N A Query_1 849 TCCCGATGGGAAAGTCGTTGCAACGAAGGATAATACCCGTAAGATTAATCATGGTCAGCCTTTTGAACAAAACTTCCTGGTGAATGCTCC 938 2 648 .......................................................................................... 737 2 673 ............ 662 P C L W S P E T P Y L Y K A V S K I Y A D G K Q T D E Y T T Query_1 939 TTGCTTGTGGTCGCCGGAGACACCTTATTTATATAAAGCTGTTTCTAAAATCTATGCCGATGGCAAGCAAACGGATGAATACACTACTCG 1028 2 738 .......................................................................................... 827 R F G I R S I E I I A D K G F F L N G K H R K F Q G V C N H Query_1 1029 TTTCGGCATCCGCAGCATAGAAATCATTGCCGACAAAGGATTTTTCCTGAACGGTAAGCATCGCAAGTTCCAGGGGGTGTGCAATCACCA 1118 2 828 .......................................................................................... 917 H D L G P L G A A I N V A A L R R Q L T M L K D M G C D A I Query_1 1119 CGATCTTGGTCCGTTAGGCGCTGCCATCAATGTTGCTGCATTGCGCCGTCAACTTACGATGCTGAAAGATATGGGTTGTGATGCCATCCG 1208 2 918 .......................................................................................... 1007 R T A H N M P A P E L V Q L C D E M G F M M M L E P F D E W Query_1 1209 CACCGCTCACAATATGCCGGCACCGGAGTTAGTGCAACTTTGTGATGAAATGGGTTTTATGATGATGCTGGAACCTTTCGACGAATGGGA 1298 2 1008 .......................................................................................... 1097 D I A K C E N G Y H R Y F N E W A E R D M I N M L H Q F R N Query_1 1299 CATTGCCAAATGTGAGAATGGCTATCACCGTTATTTCAACGAGTGGGCAGAACGTGATATGATAAATATGTTGCATCAGTTCCGCAACAA 1388 2 1098 .......................................................................................... 1187 0 27 ........-................................................... 85 N P C V V M W S I G N E V P T Q C S P V G Y K V A S F L Q D Query_1 1389 TCCTTGTGTCGTAATGTGGAGTATCGGTAATGAAGTTCCTACCCAATGTAGTCCCGTAGGCTATAAAGTCGCTTCTTTCTTGCAGGATAT 1478 2 1188 .......................................................................................... 1277 0 86 .......................................................................................... 175 I C H R E D P T R P V T C G M D Q V T C V L A N G F A A M I Query_1 1479 CTGTCATCGTGAAGATCCGACACGTCCTGTTACTTGCGGCATGGATCAGGTGACTTGTGTTCTTGCTAATGGTTTTGCCGCCATGATTGA 1568 2 1278 .......................................................................................... 1367 0 176 .......................................................................................... 265 D V P G F N A R A H R Y L E A Y E L L P Q N I V L G S E T S Query_1 1569 TGTGCCCGGTTTTAATGCTCGCGCACACCGTTATCTGGAAGCTTATGAACTGTTGCCGCAGAATATAGTACTTGGTTCTGAAACATCCTC 1658 2 1368 ................TA........................................................................ 1457 0 266 .......................................................................................... 355 S T V S S R G V Y K F P V E K R G D A K Y D D H Q S S G Y D Query_1 1659 TACCGTTAGTTCTCGTGGCGTATATAAATTTCCTGTAGAGAAACGCGGGGATGCGAAGTACGATGATCACCAGTCTTCCGGATATGACTT 1748 2 1458 .......................................................................................... 1547 0 356 .......................................................................................... 445 L E H C A W S N V P D E D F A L A D D Y D W T I G Q F V W T Query_1 1749 GGAGCATTGTGCCTGGTCTAATGTTCCAGATGAAGATTTTGCTTTAGCGGATGATTATGACTGGACTATCGGTCAATTCGTTTGGACAGG 1838 2 1548 .......................................................................................... 1637 0 446 .......................................................................................... 535 G F D Y L G E P S P Y D T D A W P S H S S L F G I I D L A S Query_1 1839 ATTCGATTATCTGGGTGAGCCTTCTCCTTATGATACGGATGCATGGCCAAGTCATAGCTCTTTGTTTGGTATCATTGACCTTGCCAGTTT 1928 2 1638 .......................................................................................... 1727 0 536 .......................................................................................... 625 L P K D R Y Y L Y R S L W N K N V N T L H I L P H W T W P G Query_1 1929 GCCAAAAGACCGCTACTATCTGTACCGTAGTCTTTGGAATAAGAATGTGAATACACTCCATATACTTCCTCACTGGACATGGCCGGGTAG 2018 2 1728 .......................................................................................... 1817 0 626 .......................................................................................... 715 R E G E N T P V F V Y T N Y P A A E L F V N G K S Y G K Q H Query_1 2019 GGAAGGAGAGAATACTCCTGTCTTTGTTTACAC-AAACTATCCTGCTGCCGAACTTTTCGTTAATGGAAAAAGCTATGGTAAACAGCATA 2107 2 1818 .................................-........................................................ 1906 0 716 .................................A........................................................ 805 K L T A E E S K A I Q D K D T L A L Q R R Y R L M W M D V P Query_1 2108 AACTGACAGCCGAAGAGAGTAAAGCTATTCAGGACAAAGATACACTTGCCCTCCAGCGTCGTTACCGCCTGATGTGGATGGACGTTCCTT 2197 2 1907 .......................................................................................... 1996 0 806 .......................................................................................... 895 Y E P G E V K V V A Y D A S G K P A E E K V V R T S G K Query_1 2198 ATGAGCC-GGGTGAAGTG-AAAGTGGTGGC-TTACGATG-CTTCCGGCAAACCTGCTGAAGAAAAAGTAG-TTCGTACTT-CCGGCAAAC 2281 2 1997 .......-..........-...........-........-..............................-.........-......... 2080 2 970 .-........-.. 980 0 896 .......G..........A...........T........NN.................N...........T.........N......... 985 P H H L E V I A D R D Q L T A D G K D L A Y I T V R V V Query_1 2282 C-TCATCATCTGG-AAGTCATT-GCTGACCGTGACCAACTCACTGCCGAT-GGTAAAGATTTGGCATACATCACTGTTCGTGTGG-TTGA 2366 2 2081 .-...........-........-...........................-..................................-.... 2165 0 986 .T.....N.....A........NN..........................G..N..............................NT.... 1075 D K D G N L C P A D N R L V N F T V K G A G R Y R A A A N G Query_1 2367 TAAAGACGGAAACCTTTG-TCCTGCT-GATAATCGTCTTGTAAACTTTACGGTGAAAGGCGCGGGGCGTTATCGTGCTGCCGCTAATGGA 2454 2 2166 ..................-.......-............................................................... 2253 0 1076 AT.....N..........T.......NN...N....N.......-N.............-NNN...NNN.NN..N....-........AG 1162 D A T S L D L F H L P K M P A F S G Q L T A I V Q M T E Q Query_1 2455 GATGCAAC-TTCACTTGATTTATTCCACTTGCCGAAGATGCCCGCTTTCAGTGGTCAGCTGACAGCCATTGTTCAAATGACCGAACAGCC 2543 2 2254 ........-................................................................................. 2342 0 1163 A....N..T.....-....NNN...ACA........ 1197 P G E I I F E A K A K G V K S G K L V L R S V R E Query_1 2544 CGGTGAAATTATTTTCGAGGCTAAGGCTAAAGGGGTGAAATCTGGTAAGCTTGTGCTGAGGTCTGTTAGAGAG 2616 2 2343 ......................................................................... 2415 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 14211210 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 5,121 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200122.106 (CMR200122.20-750.P(750).D282A.pMCSG68)
TQVANKGSDAATEKKVESLLSRMTLEEKIGQMNQITSYGNIEDMSSLIKKGEVGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQIAKDGARVAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKDVLRGEWGFDGLVVTDWASASEMISHGFAADSKEVAMKSVNAGVDMEMVSYTFVKELPALIKEGKVKESTIDEAVRNILRVKYRLGLFDVPYVDEKQPSVMYDPSHLKVAKQAAVESAILLKNDKEVLPLQESLKTIAVVGPMANAPYEQLGTWIFDGEKAHTQTPLNAIKEIVGDKVQVIYEPGLAYSREKNPAGVAKAAAVAARADVILAFVGEEAILSGEAHCLADLNLQGDQSALITALAKTGKPVVTIVMAGRPLTIGQEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKTPVTFPKMVGQIPVYYAHNNTGRPATRNEVLLDDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNVKLSSASLKKDDVLTVTFDLENTGKYKGTEVAQLYIQDKVGSVTRPVKELKRFTRVTLKPGEKKNVSFELPVSELAFWNIDMVKVVEPGDFGLWVATDSQSGEEVFFKVVD
ACACAAGTGGCAAATAAAGGTAGCGATGCGGCAACCGAGAAAAAAGTAGAGTCTCTTTTATCCAGAATGACCCTTGAAGAGAAAATCGGTCAGATGAACCAGATTACCTCTTACGGGAATATTGAGGATATGAGTAGTTTAATTAAGAAAGGTGAAGTCGGGTCTATCCTGAATGAGGTGGATCCGGTACGTATTAATGCGTTGCAACGCGTAGCGATGGAGGAGTCCCGGTTGGGAATCCCTTTGTTGATAGCTCGCGATGTTATTCACGGGTTTAAAACCATTTTTCCCATCCCATTGGGACAAGCGGCTTCGTTCAATCCGCAGATTGCGAAAGACGGTGCACGGGTAGCGGCTATTGAGGCTTCTTCCGTAGGTATCCGTTGGACTTTTGCACCGATGATCGACATTGCCCGTGATCCTCGCTGGGGGCGCATTGCCGAAGGATGTGGTGAAGACACTTACCTGACTTCTGTAATGGGAGCTGCCATGGTAGAAGGTTTTCAGGGAGATTCTTTGAATAGTCCCACTTCCATAGCTGCCTGTCCTAAACATTTTGTGGGCTATGGTGCAGCTGAAGGCGGACGTGACTATAATTCGACATTTATTCCTGAACGTCGCCTGCGTAATGTTTACTTGCCACCGTTTGAAGCGGCAACGAAAGCGGGTGCAGCTACGTTTATGACTTCCTTTAATGATAATGATGGGATACCCTCTACCGGAAATGCTTTCATATTGAAAGATGTGCTTCGTGGCGAGTGGGGATTTGATGGTTTGGTAGTGACAGACTGGGCTTCTGCCAGCGAAATGATAAGTCATGGTTTTGCTGCCGATTCTAAAGAGGTAGCCATGAAATCAGTGAATGCTGGGGTGGATATGGAAATGGTAAGTTATACCTTTGTAAAAGAATTGCCTGCATTGATAAAAGAAGGAAAGGTGAAAGAAAGCACCATTGATGAAGCCGTTCGTAATATATTGCGCGTCAAGTATCGTCTGGGATTGTTTGATGTTCCTTATGTAGATGAAAAGCAACCCTCTGTCATGTATGATCCTTCTCATCTGAAAGTAGCTAAGCAGGCTGCTGTAGAATCGGCTATCCTGTTGAAGAATGATAAAGAAGTACTGCCGTTACAGGAGTCTCTGAAAACCATTGCTGTGGTAGGACCTATGGCCAATGCGCCTTATGAACAATTGGGTACCTGGATCTTTGATGGTGAGAAAGCTCATACTCAGACACCACTGAATGCTATTAAGGAAATAGTTGGCGACAAAGTACAGGTGATTTATGAACCCGGATTAGCTTATAGCCGTGAGAAAAATCCGGCAGGCGTAGCAAAAGCTGCTGCTGTTGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAAGCCATTCTTTCGGGTGAAGCACACTGTCTGGCAGATTTGAATCTTCAGGGTGATCAAAGTGCTTTGATTACGGCTTTGGCTAAGACAGGTAAACCTGTAGTAACCATTGTGATGGCAGGTCGTCCGTTGACTATCGGTCAGGAAGTGGAAGAATCAACAGCTGTTCTTTATTCATTCCATCCGGGTACGATGGGTGGACCGGCATTGGCCGATCTGCTGTGGGGTAAGGCGGTTCCAAGTGGAAAAACACCGGTTACTTTCCCGAAGATGGTAGGACAAATTCCGGTATATTATGCTCATAACAATACCGGGCGGCCGGCTACACGTAATGAGGTGTTGCTGGATGATATTGCTGTTGAGGCTGGACAAACTTCATTGGGATGTACTTCTTTCTATATGGATGCCGGTTTTGATCCTTTATTCCCATTTGGCTATGGCTTGTCGTATACAACGTTCAAGTATAGTAATGTCAAACTTTCATCAGCGTCATTGAAGAAAGATGATGTATTGACTGTGACATTTGATCTGGAAAATACAGGTAAATATAAGGGGACGGAAGTTGCTCAATTGTATATACAAGATAAGGTTGGTTCTGTAACTCGTCCGGTGAAAGAACTGAAACGTTTTACTCGGGTAACCTTGAAACCGGGCGAGAAAAAGAATGTTTCGTTTGAACTACCCGTTAGTGAACTTGCATTTTGGAACATCGATATGGTGAAAGTTGTGGAACCCGGAGACTTTGGACTTTGGGTGGCAACAGACAGCCAATCGGGAGAAGAAGTTTTCTTTAAGGTGGTAGAT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,138 total letters Query= CMR200122.106 Length=2598 Score E Sequences producing significant alignments: (Bits) Value wt 3947 0.0 11B_B8_CMR200122.106_B08.seq 699 0.0 08B_B8_CMR200122.106_B08.seq 446 4e-128 Q F E K G T E N L Y F Q S N A T Q V A N K G S D A A T E K Query_1 157 CAGTTCG--AAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCACACAAGTGGCAAATAAAGGTAGCGATGCGGCAACCGAGAAAA 244 2 1 ........................................... 43 1 249 ............ 260 1 917 ..N....NA........... 898 K V E S L L S R M T L E E K I G Q M N Q I T S Y G N I E Query_1 245 AAGTAGAGTCTCTTTTATCCAGAATGACCCTTGAAGAGAAAATCGG-TCAGA-TGAA-CCAG-ATTACCT-CTTACGGGAATATT-GAGG 328 2 44 ..............................................-.....-....-....-.......-..............-.... 127 1 823 .........G.....A....N....N...T...N.....N.T......C.... 771 D M S S L I K K G E V G S I L N E V D P V R I N A L Q R Query_1 329 ATATGAGTAGTTTA-ATTAAGAAAGGTGAAGT-CGGGT-CTATCCTGAATGAGGTGGATC-CGGTACGTATTAATGCGTTGCAACGC-GT 413 2 128 ..............-.................-.....-.....................-..........................-.. 212 2 210 ............ 199 1 770 ...A..CNTN...NG...............A.T...N.T.C...NG........N.....N....GN.........N.C.A..G...CN. 681 V A M E E S R L G I P L L I A R D V I H G F K T I F P I P L Query_1 414 AGCGATGGAGGAGTCCCGGTTGGGAATCCCTTTGTTGATAGCTCGCGATGTTATTCACGGGTTTAAAACCATTTTTCCCATCCCATTGGG 503 2 213 .......................................................................................... 302 1 680 N..A.....A..A....NT.....T..T.....A........G..N.....C.................A.....C..T..T..CN.... 591 0 106 ........... 116 G Q A A S F N P Q I A K D G A R V A A I E A S S V G I R W T Query_1 504 ACAAGCGGCTTCGTTCAATCCGCAGATTGCGAAAGACGGTGCACGGG-TAGCGGCTATTGAGGCTTCTTCCGTAGGTATCCGTTGGACTT 592 2 303 ...............................................-.......................................... 391 1 590 .......N.................G.A.................A.A....A........A.....G..TN..........G....... 501 F A P M I D I A R D P R W G R I A E G C G E D T Y L T S V M Query_1 593 TTGCACCGATGATCGACATTGCCCGTGATCCTCGCTGGGGGCGCATTGCCGAAGGATGTGGTGAAGACACTTACCTGACTTCTGTAATGG 682 2 392 .......................................................................................... 481 1 500 .......A........T........C..............A..T.....N.....G...........T..G.....T..N..C....... 411 G A A M V E G F Q G D S L N S P T S I A A C P K H F V G Y G Query_1 683 GAGCTGCCATGGTAGAAGGTTTTCAGGGAGATTCTTTGAATAGTCCCACTTCCATAGCTGCCTGTCCTAAACATTTTGTGGGCTATGGTG 772 2 482 .......................................................................................... 571 1 410 ....A..T....N.....................GC..........T.....A..TN.A..T.NC..............A..N.TC.... 321 A A E G G R D Y N S T F I P E R R L R N V Y L P P F E A A Query_1 773 CAGCTGAAGGCGGACGTGACTATAATTCGACATTTATTCCTGAACGTCGCCTGCGTAATGTTTACTTGCCACCGT--TTGAAGCGGCAAC 860 2 572 ...........................................................................--............. 659 1 320 ....C.....A........T........C..G..C.....C........T.....C........T........T.--.......T..C.. 233 0 11 ...A.A...C.....CN.T.N...G..C.CC..NNN..T..C.. 54 T K A G A A T F M T S F N D N D G I P S T G N A F I L Query_1 861 GAAAGCGGG-TGCAG-CTACG-TTTATGACTTCC-TTTAATG-ATAATGATGGGATACCCT-CT-ACCGGA-AAT-GCTTTCATATTGAA 941 2 660 .........-.....-.....-............-.......-..................-..-......-...-.............. 740 1 232 C........-.....-.CN..-.N.........A-.......-..........A..C..T.-..A.....G-...C.....T..T..... 150 0 55 C.N..N...GN....T.CN..C..N.....N.N.N.......C...N......A..C..T.T..-.....GC...-.....T..T...N. 142 K D V L R G E W G F D G L V V T A W A S A S E M I S Query_1 942 AGATGTGCTT-CG-TG-GCGA-GTGGGGA-TTTGA-TGGTTTGGT-AGTGA-CAG-CTTGGGCTTCTGCC-AGCG--AAATGATAA-GT- 1017 2 741 ..........-..-..-....-.......-.....-.........-.....-...-AC............-....--.........-..- 816 1 149 GA....A..NC..-..AT...C.......CN....C........NA.....G...T...........N..A....AT..GN.....C..C 61 0 143 GA....A..C-..N..-A...-.......-.....-...C.....-..N..-...-..............-....--..N....C.-.C- 219 H G F A A D S K E V A M K S V N A G V D M E M V S Y T F V K Query_1 1018 CATGGTTTTGCTGCCGATTCTAAAGAGGTAGCCATGAAATCAGTGAATGCTGGGGTGGATATGGAAATGGTAAGTTATACCTTTGTAAAA 1107 2 817 .......................................................................................... 906 1 60 ........... 50 0 220 ...........C....G...A.....A..G..A....N...T........N..A..A..............G.....C..T.....G..N 309 E L P A L I K E G K V K E S T I D E A V R N I L R V K Y R L Query_1 1108 GAATTGCCTGCATTGATAAAAGAAGGAAAGGTGAAAGAAAGCACCATTGATGAAGCCGTTCGTAATATATTGCGCGTCAAGTATCGTCTG 1197 2 907 .......................................................................................... 996 1 750 ........... 740 0 310 ...C....G.A...AG.GN....N...........N........T..C.....G..T...........T.N...TA.A........NT.A 399 G L F D V P Y V D E K Q P S V M Y D P S H L K V A K Q A A Query_1 1198 GGATTGTTTGATGTTCCTTATGTAGATGAAAAGCAACCCTCTGTCATGTATGATCCTTCTCATCTGAAAGTAGC-TAAGCAGGCTGCTGT 1286 2 997 ..........................................................................-............... 1085 0 400 ............AC................C.A.N.A.A.............C....N.....T..G..NC.N.N...N..A..CN...A 489 V E S A I L L K N D K E V L P L Q E S L K T I A V V G P M Query_1 1287 AGAATCG-GCTATCCTGTTGAAGAATGATAAAGAAGTACTGCCGTTACAG--GAGTCTCTGAAAACCAT-TGCTGTGGTAGGACCTATGG 1372 2 1086 .......-..........................................--.................-.................... 1171 0 490 T.....NCA....T...........N.....G...N.GT...........CCN.TCN.G.......NG.C...NNN..C.C....N.... 579 A N A P Y E Q L G T W I F D G E K A H T Q T P L N A I Query_1 1373 CCAATGCGCCTTATGAAC-AATTGGGTACCT-GGATCTTTGATGGTGAGA-AAGCTCATACTCA-GACACC-ACTGAA-TGCTA--TTAA 1454 2 1172 ..................-............-..................-.............-......-......-.....--.... 1253 0 580 .T..A..A.........NTNNNA.N....T.N....NN.......N..N.N..N...GNTA...N.N.T..GTN....CCN...NC.A.. 669 K E I V G D K V Q V I Y E P G L A Y S R E K N P A G V A K Query_1 1455 GGAAATAGTTGG--CGACAAAGTACAGGT-GATTTATGAACCCGGATTAGCTTATAGCCGTGAGAAAAATCCGGCAGGCGTAGCAAAAGC 1541 2 1254 ............--...............-............................................................ 1340 0 670 .A....G...C.NC.N........N.N..N.............. 713 A A A V A A R A D V I L A F V G E E A I L S G E A H C L A D Query_1 1542 TGCTGCTGTTGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAAGCCATTCTTTCGGGTGAAGCACACTGTCTGGCAGATTT 1631 2 1341 .......................................................................................... 1430 2 1363 ............ 1352 L N L Q G D Q S A L I T A L A K T G K P V V T I V M A G R P Query_1 1632 GAATCTTCAGGGTGATCAAAGTGCTTTGATTACGGCTTTGGCTAAGACAGGTAAACCTGTAGTAACCATTGTGATGGCAGGTCGTCCGTT 1721 2 1431 .......................................................................................... 1520 L T I G Q E V E E S T A V L Y S F H P G T M G G P A L A D L Query_1 1722 GACTATCGGTCAGGAAGTGGAAGAATCAACAGCTGTTCTTTATTCATTCCATCCGGGTACGATGGGTGGACCGGCATTGGCCGATCTGCT 1811 2 1521 .......................................................................................... 1610 L W G K A V P S G K T P V T F P K M V G Q I P V Y Y A H N N Query_1 1812 GTGGGGTAAGGCGGTTCCAAGTGGAAAAACACCGGTTACTTTCCCGAAGATGGTAGGACAAATTCCGGTATATTATGCTCATAACAATAC 1901 2 1611 .......................................................................................... 1700 T G R P A T R N E V L L D D I A V E A G Q T S L G C T S F Y Query_1 1902 CGGGCGGCCGGCTACACGTAATGAGGTGTTGCTGGATGATATTGCTGTTGAGGCTGGACAAACTTCATTGGGATGTACTTCTTTCTATAT 1991 2 1701 .......................................................................................... 1790 M D A G F D P L F P F G Y G L S Y T T F K Y S N V K L S S A Query_1 1992 GGATGCCGGTTTTGATCCTTTATTCCCATTTGGCTATGGCTTGTCGTATACAACGTTCAAGTATAGTAATGTCAAACTTTCATCAGCGTC 2081 2 1791 .......................................................................................... 1880 S L K K D D V L T V T F D L E N T G K Y K G T E V A Q L Y I Query_1 2082 ATTGAAGAAAGATGATGTATTGACTGTGACATTTGATCTGGAAAATACAGGTAAATATAAGGGGACGGAAGTTGCTCAATTGTATATACA 2171 2 1881 .......................................................................................... 1970 2 1971 ........... 1961 Q D K V G S V T R P V K E L K R F T R V T L K P G E K K N V Query_1 2172 AGATAAGGTTGGTTCTGTAACTCGTCCGGTGAAAGAACTGAAACGTTTTACTCGGGTAACCTTGAAACCGGGCGAGAAAAAGAATGTTTC 2261 2 1971 .......................................................................................... 2060 2 1960 . 1960 S F E L P V S E L A F W N I D M V K V V E P G D F G L W V A Query_1 2262 GTTTGAACTACCCGTTAGTGAACTTGCATTTTGGAACATCGATATGGTGAAAGTTGTGGAACCCGGAGACTTTGGACTTTGGGTGGCAAC 2351 2 2061 .......................................................................................... 2150 T D S Q S G E E V F F K V V D Query_1 2352 AGACAGCCAATCGGGAGAAGAAGTTTTCTTTAAGGTGGTAGAT 2394 2 2151 ........................................... 2193 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 13129547 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 5,138 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200122.107 (CMR200122.20-750.P(750).D282A.pMCSG68)
TQVANKGSDAATEKKVESLLSRMTLEEKIGQMNQITSYGNIEDMSSLIKKGEVGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQIAKDGARVAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKDVLRGEWGFDGLVVTDWASASEMISHGFAADSKEVAMKSVNAGVDMEMVSYTFVKELPALIKEGKVKESTIDEAVRNILRVKYRLGLFDVPYVDEKQPSVMYDPSHLKVAKQAAVESAILLKNDKEVLPLQESLKTIAVVGPMANAPYEQLGTWIFDGEKAHTQTPLNAIKEIVGDKVQVIYEPGLAYSREKNPAGVAKAAAVAARADVILAFVGEEAILSGEAHCLADLNLQGDQSALITALAKTGKPVVTIVMAGRPLTIGQEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKTPVTFPKMVGQIPVYYAHNNTGRPATRNEVLLDDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNVKLSSASLKKDDVLTVTFDLENTGKYKGTEVAQLYIQDKVGSVTRPVKELKRFTRVTLKPGEKKNVSFELPVSELAFWNIDMVKVVEPGDFGLWVATDSQSGEEVFFKVVD
ACACAAGTGGCAAATAAAGGTAGCGATGCGGCAACCGAGAAAAAAGTAGAGTCTCTTTTATCCAGAATGACCCTTGAAGAGAAAATCGGTCAGATGAACCAGATTACCTCTTACGGGAATATTGAGGATATGAGTAGTTTAATTAAGAAAGGTGAAGTCGGGTCTATCCTGAATGAGGTGGATCCGGTACGTATTAATGCGTTGCAACGCGTAGCGATGGAGGAGTCCCGGTTGGGAATCCCTTTGTTGATAGCTCGCGATGTTATTCACGGGTTTAAAACCATTTTTCCCATCCCATTGGGACAAGCGGCTTCGTTCAATCCGCAGATTGCGAAAGACGGTGCACGGGTAGCGGCTATTGAGGCTTCTTCCGTAGGTATCCGTTGGACTTTTGCACCGATGATCGACATTGCCCGTGATCCTCGCTGGGGGCGCATTGCCGAAGGATGTGGTGAAGACACTTACCTGACTTCTGTAATGGGAGCTGCCATGGTAGAAGGTTTTCAGGGAGATTCTTTGAATAGTCCCACTTCCATAGCTGCCTGTCCTAAACATTTTGTGGGCTATGGTGCAGCTGAAGGCGGACGTGACTATAATTCGACATTTATTCCTGAACGTCGCCTGCGTAATGTTTACTTGCCACCGTTTGAAGCGGCAACGAAAGCGGGTGCAGCTACGTTTATGACTTCCTTTAATGATAATGATGGGATACCCTCTACCGGAAATGCTTTCATATTGAAAGATGTGCTTCGTGGCGAGTGGGGATTTGATGGTTTGGTAGTGACAGACTGGGCTTCTGCCAGCGAAATGATAAGTCATGGTTTTGCTGCCGATTCTAAAGAGGTAGCCATGAAATCAGTGAATGCTGGGGTGGATATGGAAATGGTAAGTTATACCTTTGTAAAAGAATTGCCTGCATTGATAAAAGAAGGAAAGGTGAAAGAAAGCACCATTGATGAAGCCGTTCGTAATATATTGCGCGTCAAGTATCGTCTGGGATTGTTTGATGTTCCTTATGTAGATGAAAAGCAACCCTCTGTCATGTATGATCCTTCTCATCTGAAAGTAGCTAAGCAGGCTGCTGTAGAATCGGCTATCCTGTTGAAGAATGATAAAGAAGTACTGCCGTTACAGGAGTCTCTGAAAACCATTGCTGTGGTAGGACCTATGGCCAATGCGCCTTATGAACAATTGGGTACCTGGATCTTTGATGGTGAGAAAGCTCATACTCAGACACCACTGAATGCTATTAAGGAAATAGTTGGCGACAAAGTACAGGTGATTTATGAACCCGGATTAGCTTATAGCCGTGAGAAAAATCCGGCAGGCGTAGCAAAAGCTGCTGCTGTTGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAAGCCATTCTTTCGGGTGAAGCACACTGTCTGGCAGATTTGAATCTTCAGGGTGATCAAAGTGCTTTGATTACGGCTTTGGCTAAGACAGGTAAACCTGTAGTAACCATTGTGATGGCAGGTCGTCCGTTGACTATCGGTCAGGAAGTGGAAGAATCAACAGCTGTTCTTTATTCATTCCATCCGGGTACGATGGGTGGACCGGCATTGGCCGATCTGCTGTGGGGTAAGGCGGTTCCAAGTGGAAAAACACCGGTTACTTTCCCGAAGATGGTAGGACAAATTCCGGTATATTATGCTCATAACAATACCGGGCGGCCGGCTACACGTAATGAGGTGTTGCTGGATGATATTGCTGTTGAGGCTGGACAAACTTCATTGGGATGTACTTCTTTCTATATGGATGCCGGTTTTGATCCTTTATTCCCATTTGGCTATGGCTTGTCGTATACAACGTTCAAGTATAGTAATGTCAAACTTTCATCAGCGTCATTGAAGAAAGATGATGTATTGACTGTGACATTTGATCTGGAAAATACAGGTAAATATAAGGGGACGGAAGTTGCTCAATTGTATATACAAGATAAGGTTGGTTCTGTAACTCGTCCGGTGAAAGAACTGAAACGTTTTACTCGGGTAACCTTGAAACCGGGCGAGAAAAAGAATGTTTCGTTTGAACTACCCGTTAGTGAACTTGCATTTTGGAACATCGATATGGTGAAAGTTGTGGAACCCGGAGACTTTGGACTTTGGGTGGCAACAGACAGCCAATCGGGAGAAGAAGTTTTCTTTAAGGTGGTAGAT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,148 total letters Query= CMR200122.107 Length=2598 Score E Sequences producing significant alignments: (Bits) Value wt 3947 0.0 08C_C8_CMR200122.107_C08.seq 760 0.0 11C_C8_CMR200122.107_C08.seq 376 7e-107 T Q V A N K G S D A A T E K K V E S L L S R M T L E E K I G Query_1 202 ACACAAGTGGCAAATAAAGGTAGCGATGCGGCAACCGAGAAAAAAGTAGAGTCTCTTTTATCCAGAATGACCCTTGAAGAGAAAATCGGT 291 2 1 .......................................................................................... 90 Q M N Q I T S Y G N I E D M S S L I K K G E V G S I L N E V Query_1 292 CAGATGAACCAGATTACCTCTTACGGGAATATTGAGGATATGAGTAGTTTAATTAAGAAAGGTGAAGTCGGGTCTATCCTGAATGAGGTG 381 2 91 .......................................................................................... 180 1 729 ..T..C...NC..NN.N.N.A 709 D P V R I N A L Q R V A M E E S R L G I P L L I A R D V I Query_1 382 GATCCGGTACGTATTAATGCGTTGCAACGCGTAGCGATGGAGGAGTCCCGGTTGGGAATCCCTTTGT-TGATAGCTCGCG-ATGTTATTC 469 2 181 ...................................................................-............-......... 268 2 210 ............ 199 1 708 .......NNGA......N...C.A..N...ANGN.AN....A..A.....N...N.T.CN....NA.AN.N.N..G..N.N.N..C.... 619 H G F K T I F P I P L G Q A A S F N P Q I A K D G A R V A Query_1 470 ACGGGTTTAAAACCATTTTTCCCATCCCATTGGGACAAGCGGCTTCGTT--CAATCCGCAGATTGCGAAAGACG-GTGCACGGGTAGCGG 556 2 269 .................................................--.......................-............... 355 1 618 .............A.....C..N..TN.C.........NN.NN...NN.TG..........G.A.......C.AA.NN.....A....A. 529 A I E A S S V G I R W T F A P M I D I A R D P R W G R I A Query_1 557 CTATTGAGGCTTCTTCCGTAGGTATC-CGTTGGACTTTTGCACCGA-TGATCGACATTGCCCGTGAT-CCTCGCTGGGGGCGCATTGCCG 643 2 356 ..........................-...................-....................-...................... 442 1 528 .......A.A...G.NT..N...N..G..G.......A......A.A..N.TA.T.AA.N...C...G...........A..N.....N. 439 E G C G E D T Y L T S V M G A A M V E G F Q G D S L N S P T Query_1 644 AAGGATGTGGTGAAGACACTTACCTGACTTCTGTAATGGGAGCTGCCATGGTAGAAGGTTTTCAGGGAGATTCTTTGAATAGTCCCACTT 733 2 443 .......................................................................................... 532 1 438 ....N...........T..G.....T.....C.........N.N..T................N.........GN..........T.... 349 S I A A C P K H F V G Y G A A E G G R D Y N S T F I P E R R Query_1 734 CCATAGCTGCCTGTCCTAAACATTTTGTGGGCTATGGTGCAGCTGAAGGCGGACGTGACTATAATTCGACATTTATTCCTGAACGTCGCC 823 2 533 .......................................................................................... 622 1 348 .A.NT..A..A.NC...NN......A..AN.T..C.NN...N.C.....A.....N..N........CG.G..C.....C..N.N...T. 259 L R N V Y L P P F E A A T K A G A A T F M T S F N D N D G Query_1 824 TGCGTAATGTTTACTTGCCACCGTTTGAAGCGGCAACGAAAG-CGGG-TGCAGCT-ACG-TTTATGACTTCCTTTAATGATAATGATGGG 909 2 623 ..........................................-....-.......-...-.............................. 708 0 44 ........T..C..C....G....C......CC...-...........A.................A 109 1 258 ....C..GN.N..TN....T..T.AN.....T..C..C.C..-....-......C-...CA...N.N....AN.N........N..N..A 172 I P S T G N A F I L K D V L R G E W G F D G L V V T Query_1 910 ATACCCT-CTACCGGAAATGC-TTTCATAT-TGAA-AGATGTGCT-TCGTG-GCGA-GTGGGG-ATTTGA-TGGTTTGGT-AGTGACAG- 988 2 709 .......-.............-........-....-.........-.....-....-......-......-.........-........- 787 0 110 ..C....T.......G.....A...T..T.-....-GA....A..-C....-A...-......-......-.........-........- 190 1 171 ..C..T.-..N...NG...N.-.C.T.NN.G....G.AN......GC...NAC...T.....NA...N..C...NN....N......N.A 84 A W A S A S E M I S H G F A A D S K E V A M K S V N A G V Query_1 989 -CTTGGGCTTCTGCCAGCGAAATGA-TAAGTCATGGTTTTGCTGCCGATTCTAAAGAGGTAGCCATGAAATCAGTGAATGCTGGGGTGGA 1076 2 788 -AC......................-................................................................ 875 0 191 -........................-....C...........C....G...A.....A..G..A........T........A..A..A.. 278 1 83 A...-T.N...AN............C.......C.N...G.. 43 D M E M V S Y T F V K E L P A L I K E G K V K E S T I D E A Query_1 1077 TATGGAAATGGTAAGTTATACCTTTGTAAAAGAATTGCCTGCATTGATAAAAGAAGGAAAGGTGAAAGAAAGCACCATTGATGAAGCCGT 1166 2 876 .......................................................................................... 965 0 279 ............G.....C..T.....G..G...C....G.A...AG.G.....G...........G........T..C.....G..T.. 368 V R N I L R V K Y R L G L F D V P Y V D E K Q P S V M Y D P Query_1 1167 TCGTAATATATTGCGCGTCAAGTATCGTCTGGGATTGTTTGATGTTCCTTATGTAGATGAAAAGCAACCCTCTGTCATGTATGATCCTTC 1256 2 966 .......................................................................................... 1055 0 369 .........T.....TA.A........NT.A............ACA...............C.A...A.A.............C...... 458 S H L K V A K Q A A V E S A I L L K N D K E V L P L Q E S L Query_1 1257 TCATCTGAAAGTAGCTAAGCAGGCTGCTGTAGAATCGGCTATCCTGTTGAAGAATGATAAAGAAGTACTGCCGTTACAGGAGTCTCTGAA 1346 2 1056 .......................................................................................... 1145 0 459 ....T..G...C.........A..C.....T......N....T.................G.....GT...........CCA...G.... 548 K T I A V V G P M A N A P Y E Q L G T W I F D G E K A H T Q Query_1 1347 AACCATTGCTGTGGTAGGACCTATGGCCAATGCGCCTTATGAACAATTGGGTACCTGGATCTTTGATGGTGAGAAAGCTCATACTCAGAC 1436 2 1146 .......................................................................................... 1235 0 549 ...TG....N......N..........T.....A........N.NG..A.....N.....A........N..........G.....N... 638 T P L N A I K E I V G D K V Q V I Y E P G L A Y S R E K N P Query_1 1437 ACCACTGAATGCTATTAAGGAAATAGTTGGCGACAAAGTACAGGTGATTTATGA-ACCCGGATTAGCTTATAGCCGTGAGAAAAATCCGG 1525 2 1236 ......................................................-................................... 1324 0 639 T..GT....C........A.....G......N.T....................N...G...C....A.....N..N............. 728 A G V A K A A A V A A R A D V I L A F V G E E A I L S Query_1 1526 --CAGGCGTAGC--AAAAGCTGCTG-CTGTTGCTGCACGTG-CAGATGTCATTCTTGCTTTTGT-GGGTG-AAGAAGCCATTCTTTCG-G 1606 2 1325 --..........--...........-...............-......................-.....-.................-. 1405 2 1363 .........-... 1352 0 729 GCA.ANT..G..NTN...N.NA...C.NCCC.........NC.T.................C..NN..N.A.....T.A.....C...N. 818 G E A H C L A D L N L Q G D Q S A L I T A L A K T G K P V V Query_1 1607 GTGAAGCACACTGTCTGGCAGATTTGAATCTTCAGGGTGATCAAAGTGCTTTGATTACGGCTTTGGCTAAGACAGGTAAACCTGTAGTAA 1696 2 1406 .......................................................................................... 1495 0 819 N..... 824 T I V M A G R P L T I G Q E V E E S T A V L Y S F H P G T M Query_1 1697 CCATTGTGATGGCAGGTCGTCCGTTGACTATCGGTCAGGAAGTGGAAGAATCAACAGCTGTTCTTTATTCATTCCATCCGGGTACGATGG 1786 2 1496 .......................................................................................... 1585 G G P A L A D L L W G K A V P S G K T P V T F P K M V G Q I Query_1 1787 GTGGACCGGCATTGGCCGATCTGCTGTGGGGTAAGGCGGTTCCAAGTGGAAAAACACCGGTTACTTTCCCGAAGATGGTAGGACAAATTC 1876 2 1586 .......................................................................................... 1675 P V Y Y A H N N T G R P A T R N E V L L D D I A V E A G Q T Query_1 1877 CGGTATATTATGCTCATAACAATACCGGGCGGCCGGCTACACGTAATGAGGTGTTGCTGGATGATATTGCTGTTGAGGCTGGACAAACTT 1966 2 1676 .......................................................................................... 1765 S L G C T S F Y M D A G F D P L F P F G Y G L S Y T T F K Y Query_1 1967 CATTGGGATGTACTTCTTTCTATATGGATGCCGGTTTTGATCCTTTATTCCCATTTGGCTATGGCTTGTCGTATACAACGTTCAAGTATA 2056 2 1766 .......................................................................................... 1855 S N V K L S S A S L K K D D V L T V T F D L E N T G K Y K G Query_1 2057 GTAATGTCAAACTTTCATCAGCGTCATTGAAGAAAGATGATGTATTGACTGTGACATTTGATCTGGAAAATACAGGTAAATATAAGGGGA 2146 2 1856 .......................................................................................... 1945 T E V A Q L Y I Q D K V G S V T R P V K E L K R F T R V T L Query_1 2147 CGGAAGTTGCTCAATTGTATATACAAGATAAGGTTGGTTCTGTAACTCGTCCGGTGAAAGAACTGAAACGTTTTACTCGGGTAACCTTGA 2236 2 1946 .......................................................................................... 2035 2 1971 ............ 1960 K P G E K K N V S F E L P V S E L A F W N I D M V K V V E P Query_1 2237 AACCGGGCGAGAAAAAGAATGTTTCGTTTGAACTACCCGTTAGTGAACTTGCATTTTGGAACATCGATATGGTGAAAGTTGTGGAACCCG 2326 2 2036 .......................................................................................... 2125 G D F G L W V A T D S Q S G E E V F F K V V D Query_1 2327 GAGACTTTGGACTTTGGGTGGCAACAGACAGCCAATCGGGAGAAGAAGTTTTCTTTAAGGTGGTAGAT 2394 2 2126 .................................................................... 2193 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 13155357 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 5,148 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200122.113 (CMR200122.20-750.P(750).W283F.pMCSG68)
TQVANKGSDAATEKKVESLLSRMTLEEKIGQMNQITSYGNIEDMSSLIKKGEVGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQIAKDGARVAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKDVLRGEWGFDGLVVTDWASASEMISHGFAADSKEVAMKSVNAGVDMEMVSYTFVKELPALIKEGKVKESTIDEAVRNILRVKYRLGLFDVPYVDEKQPSVMYDPSHLKVAKQAAVESAILLKNDKEVLPLQESLKTIAVVGPMANAPYEQLGTWIFDGEKAHTQTPLNAIKEIVGDKVQVIYEPGLAYSREKNPAGVAKAAAVAARADVILAFVGEEAILSGEAHCLADLNLQGDQSALITALAKTGKPVVTIVMAGRPLTIGQEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKTPVTFPKMVGQIPVYYAHNNTGRPATRNEVLLDDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNVKLSSASLKKDDVLTVTFDLENTGKYKGTEVAQLYIQDKVGSVTRPVKELKRFTRVTLKPGEKKNVSFELPVSELAFWNIDMVKVVEPGDFGLWVATDSQSGEEVFFKVVD
ACACAAGTGGCAAATAAAGGTAGCGATGCGGCAACCGAGAAAAAAGTAGAGTCTCTTTTATCCAGAATGACCCTTGAAGAGAAAATCGGTCAGATGAACCAGATTACCTCTTACGGGAATATTGAGGATATGAGTAGTTTAATTAAGAAAGGTGAAGTCGGGTCTATCCTGAATGAGGTGGATCCGGTACGTATTAATGCGTTGCAACGCGTAGCGATGGAGGAGTCCCGGTTGGGAATCCCTTTGTTGATAGCTCGCGATGTTATTCACGGGTTTAAAACCATTTTTCCCATCCCATTGGGACAAGCGGCTTCGTTCAATCCGCAGATTGCGAAAGACGGTGCACGGGTAGCGGCTATTGAGGCTTCTTCCGTAGGTATCCGTTGGACTTTTGCACCGATGATCGACATTGCCCGTGATCCTCGCTGGGGGCGCATTGCCGAAGGATGTGGTGAAGACACTTACCTGACTTCTGTAATGGGAGCTGCCATGGTAGAAGGTTTTCAGGGAGATTCTTTGAATAGTCCCACTTCCATAGCTGCCTGTCCTAAACATTTTGTGGGCTATGGTGCAGCTGAAGGCGGACGTGACTATAATTCGACATTTATTCCTGAACGTCGCCTGCGTAATGTTTACTTGCCACCGTTTGAAGCGGCAACGAAAGCGGGTGCAGCTACGTTTATGACTTCCTTTAATGATAATGATGGGATACCCTCTACCGGAAATGCTTTCATATTGAAAGATGTGCTTCGTGGCGAGTGGGGATTTGATGGTTTGGTAGTGACAGACTGGGCTTCTGCCAGCGAAATGATAAGTCATGGTTTTGCTGCCGATTCTAAAGAGGTAGCCATGAAATCAGTGAATGCTGGGGTGGATATGGAAATGGTAAGTTATACCTTTGTAAAAGAATTGCCTGCATTGATAAAAGAAGGAAAGGTGAAAGAAAGCACCATTGATGAAGCCGTTCGTAATATATTGCGCGTCAAGTATCGTCTGGGATTGTTTGATGTTCCTTATGTAGATGAAAAGCAACCCTCTGTCATGTATGATCCTTCTCATCTGAAAGTAGCTAAGCAGGCTGCTGTAGAATCGGCTATCCTGTTGAAGAATGATAAAGAAGTACTGCCGTTACAGGAGTCTCTGAAAACCATTGCTGTGGTAGGACCTATGGCCAATGCGCCTTATGAACAATTGGGTACCTGGATCTTTGATGGTGAGAAAGCTCATACTCAGACACCACTGAATGCTATTAAGGAAATAGTTGGCGACAAAGTACAGGTGATTTATGAACCCGGATTAGCTTATAGCCGTGAGAAAAATCCGGCAGGCGTAGCAAAAGCTGCTGCTGTTGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAAGCCATTCTTTCGGGTGAAGCACACTGTCTGGCAGATTTGAATCTTCAGGGTGATCAAAGTGCTTTGATTACGGCTTTGGCTAAGACAGGTAAACCTGTAGTAACCATTGTGATGGCAGGTCGTCCGTTGACTATCGGTCAGGAAGTGGAAGAATCAACAGCTGTTCTTTATTCATTCCATCCGGGTACGATGGGTGGACCGGCATTGGCCGATCTGCTGTGGGGTAAGGCGGTTCCAAGTGGAAAAACACCGGTTACTTTCCCGAAGATGGTAGGACAAATTCCGGTATATTATGCTCATAACAATACCGGGCGGCCGGCTACACGTAATGAGGTGTTGCTGGATGATATTGCTGTTGAGGCTGGACAAACTTCATTGGGATGTACTTCTTTCTATATGGATGCCGGTTTTGATCCTTTATTCCCATTTGGCTATGGCTTGTCGTATACAACGTTCAAGTATAGTAATGTCAAACTTTCATCAGCGTCATTGAAGAAAGATGATGTATTGACTGTGACATTTGATCTGGAAAATACAGGTAAATATAAGGGGACGGAAGTTGCTCAATTGTATATACAAGATAAGGTTGGTTCTGTAACTCGTCCGGTGAAAGAACTGAAACGTTTTACTCGGGTAACCTTGAAACCGGGCGAGAAAAAGAATGTTTCGTTTGAACTACCCGTTAGTGAACTTGCATTTTGGAACATCGATATGGTGAAAGTTGTGGAACCCGGAGACTTTGGACTTTGGGTGGCAACAGACAGCCAATCGGGAGAAGAAGTTTTCTTTAAGGTGGTAGAT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,625 total letters Query= CMR200122.113 Length=2598 Score E Sequences producing significant alignments: (Bits) Value wt 3947 0.0 T Q V A N K G S D A A T E K K V E S L L S R M T L E E K I G Query_1 202 ACACAAGTGGCAAATAAAGGTAGCGATGCGGCAACCGAGAAAAAAGTAGAGTCTCTTTTATCCAGAATGACCCTTGAAGAGAAAATCGGT 291 2 1 .......................................................................................... 90 Q M N Q I T S Y G N I E D M S S L I K K G E V G S I L N E V Query_1 292 CAGATGAACCAGATTACCTCTTACGGGAATATTGAGGATATGAGTAGTTTAATTAAGAAAGGTGAAGTCGGGTCTATCCTGAATGAGGTG 381 2 91 .......................................................................................... 180 D P V R I N A L Q R V A M E E S R L G I P L L I A R D V I H Query_1 382 GATCCGGTACGTATTAATGCGTTGCAACGCGTAGCGATGGAGGAGTCCCGGTTGGGAATCCCTTTGTTGATAGCTCGCGATGTTATTCAC 471 2 181 .......................................................................................... 270 2 210 ............ 199 G F K T I F P I P L G Q A A S F N P Q I A K D G A R V A A I Query_1 472 GGGTTTAAAACCATTTTTCCCATCCCATTGGGACAAGCGGCTTCGTTCAATCCGCAGATTGCGAAAGACGGTGCACGGGTAGCGGCTATT 561 2 271 .......................................................................................... 360 E A S S V G I R W T F A P M I D I A R D P R W G R I A E G C Query_1 562 GAGGCTTCTTCCGTAGGTATCCGTTGGACTTTTGCACCGATGATCGACATTGCCCGTGATCCTCGCTGGGGGCGCATTGCCGAAGGATGT 651 2 361 .......................................................................................... 450 G E D T Y L T S V M G A A M V E G F Q G D S L N S P T S I A Query_1 652 GGTGAAGACACTTACCTGACTTCTGTAATGGGAGCTGCCATGGTAGAAGGTTTTCAGGGAGATTCTTTGAATAGTCCCACTTCCATAGCT 741 2 451 .......................................................................................... 540 A C P K H F V G Y G A A E G G R D Y N S T F I P E R R L R N Query_1 742 GCCTGTCCTAAACATTTTGTGGGCTATGGTGCAGCTGAAGGCGGACGTGACTATAATTCGACATTTATTCCTGAACGTCGCCTGCGTAAT 831 2 541 .......................................................................................... 630 V Y L P P F E A A T K A G A A T F M T S F N D N D G I P S T Query_1 832 GTTTACTTGCCACCGTTTGAAGCGGCAACGAAAGCGGGTGCAGCTACGTTTATGACTTCCTTTAATGATAATGATGGGATACCCTCTACC 921 2 631 .......................................................................................... 720 G N A F I L K D V L R G E W G F D G L V V T D F A S A S E M Query_1 922 GGAAATGCTTTCATATTGAAAGATGTGCTTCGTGGCGAGTGGGGATTTGATGGTTTGGTAGTGACAGACTTTGCTTCTGCCAGCGAAATG 1011 2 721 ......................................................................GG.................. 810 I S H G F A A D S K E V A M K S V N A G V D M E M V S Y T F Query_1 1012 ATAAGTCATGGTTTTGCTGCCGATTCTAAAGAGGTAGCCATGAAATCAGTGAATGCTGGGGTGGATATGGAAATGGTAAGTTATACCTTT 1101 2 811 .......................................................................................... 900 V K E L P A L I K E G K V K E S T I D E A V R N I L R V K Y Query_1 1102 GTAAAAGAATTGCCTGCATTGATAAAAGAAGGAAAGGTGAAAGAAAGCACCATTGATGAAGCCGTTCGTAATATATTGCGCGTCAAGTAT 1191 2 901 .......................................................................................... 990 R L G L F D V P Y V D E K Q P S V M Y D P S H L K V A K Q A Query_1 1192 CGTCTGGGATTGTTTGATGTTCCTTATGTAGATGAAAAGCAACCCTCTGTCATGTATGATCCTTCTCATCTGAAAGTAGCTAAGCAGGCT 1281 2 991 .......................................................................................... 1080 A V E S A I L L K N D K E V L P L Q E S L K T I A V V G P M Query_1 1282 GCTGTAGAATCGGCTATCCTGTTGAAGAATGATAAAGAAGTACTGCCGTTACAGGAGTCTCTGAAAACCATTGCTGTGGTAGGACCTATG 1371 2 1081 .......................................................................................... 1170 A N A P Y E Q L G T W I F D G E K A H T Q T P L N A I K E I Query_1 1372 GCCAATGCGCCTTATGAACAATTGGGTACCTGGATCTTTGATGGTGAGAAAGCTCATACTCAGACACCACTGAATGCTATTAAGGAAATA 1461 2 1171 .......................................................................................... 1260 V G D K V Q V I Y E P G L A Y S R E K N P A G V A K A A A V Query_1 1462 GTTGGCGACAAAGTACAGGTGATTTATGAACCCGGATTAGCTTATAGCCGTGAGAAAAATCCGGCAGGCGTAGCAAAAGCTGCTGCTGTT 1551 2 1261 .......................................................................................... 1350 A A R A D V I L A F V G E E A I L S G E A H C L A D L N L Q Query_1 1552 GCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAAGCCATTCTTTCGGGTGAAGCACACTGTCTGGCAGATTTGAATCTTCAG 1641 2 1351 .......................................................................................... 1440 2 1363 ............ 1352 G D Q S A L I T A L A K T G K P V V T I V M A G R P L T I G Query_1 1642 GGTGATCAAAGTGCTTTGATTACGGCTTTGGCTAAGACAGGTAAACCTGTAGTAACCATTGTGATGGCAGGTCGTCCGTTGACTATCGGT 1731 2 1441 .......................................................................................... 1530 Q E V E E S T A V L Y S F H P G T M G G P A L A D L L W G K Query_1 1732 CAGGAAGTGGAAGAATCAACAGCTGTTCTTTATTCATTCCATCCGGGTACGATGGGTGGACCGGCATTGGCCGATCTGCTGTGGGGTAAG 1821 2 1531 .......................................................................................... 1620 A V P S G K T P V T F P K M V G Q I P V Y Y A H N N T G R P Query_1 1822 GCGGTTCCAAGTGGAAAAACACCGGTTACTTTCCCGAAGATGGTAGGACAAATTCCGGTATATTATGCTCATAACAATACCGGGCGGCCG 1911 2 1621 .......................................................................................... 1710 A T R N E V L L D D I A V E A G Q T S L G C T S F Y M D A G Query_1 1912 GCTACACGTAATGAGGTGTTGCTGGATGATATTGCTGTTGAGGCTGGACAAACTTCATTGGGATGTACTTCTTTCTATATGGATGCCGGT 2001 2 1711 .......................................................................................... 1800 F D P L F P F G Y G L S Y T T F K Y S N V K L S S A S L K K Query_1 2002 TTTGATCCTTTATTCCCATTTGGCTATGGCTTGTCGTATACAACGTTCAAGTATAGTAATGTCAAACTTTCATCAGCGTCATTGAAGAAA 2091 2 1801 .......................................................................................... 1890 D D V L T V T F D L E N T G K Y K G T E V A Q L Y I Q D K V Query_1 2092 GATGATGTATTGACTGTGACATTTGATCTGGAAAATACAGGTAAATATAAGGGGACGGAAGTTGCTCAATTGTATATACAAGATAAGGTT 2181 2 1891 .......................................................................................... 1980 2 1971 ............ 1960 G S V T R P V K E L K R F T R V T L K P G E K K N V S F E L Query_1 2182 GGTTCTGTAACTCGTCCGGTGAAAGAACTGAAACGTTTTACTCGGGTAACCTTGAAACCGGGCGAGAAAAAGAATGTTTCGTTTGAACTA 2271 2 1981 .......................................................................................... 2070 P V S E L A F W N I D M V K V V E P G D F G L W V A T D S Q Query_1 2272 CCCGTTAGTGAACTTGCATTTTGGAACATCGATATGGTGAAAGTTGTGGAACCCGGAGACTTTGGACTTTGGGTGGCAACAGACAGCCAA 2361 2 2071 .......................................................................................... 2160 S G E E V F F K V V D Query_1 2362 TCGGGAGAAGAAGTTTTCTTTAAGGTGGTAGAT 2394 2 2161 ................................. 2193 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 9224494 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 3,625 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200122.116 (CMR200122.20-750.P(750).F250W.pMCSG68)
TQVANKGSDAATEKKVESLLSRMTLEEKIGQMNQITSYGNIEDMSSLIKKGEVGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQIAKDGARVAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKDVLRGEWGFDGLVVTDWASASEMISHGFAADSKEVAMKSVNAGVDMEMVSYTFVKELPALIKEGKVKESTIDEAVRNILRVKYRLGLFDVPYVDEKQPSVMYDPSHLKVAKQAAVESAILLKNDKEVLPLQESLKTIAVVGPMANAPYEQLGTWIFDGEKAHTQTPLNAIKEIVGDKVQVIYEPGLAYSREKNPAGVAKAAAVAARADVILAFVGEEAILSGEAHCLADLNLQGDQSALITALAKTGKPVVTIVMAGRPLTIGQEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKTPVTFPKMVGQIPVYYAHNNTGRPATRNEVLLDDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNVKLSSASLKKDDVLTVTFDLENTGKYKGTEVAQLYIQDKVGSVTRPVKELKRFTRVTLKPGEKKNVSFELPVSELAFWNIDMVKVVEPGDFGLWVATDSQSGEEVFFKVVD
ACACAAGTGGCAAATAAAGGTAGCGATGCGGCAACCGAGAAAAAAGTAGAGTCTCTTTTATCCAGAATGACCCTTGAAGAGAAAATCGGTCAGATGAACCAGATTACCTCTTACGGGAATATTGAGGATATGAGTAGTTTAATTAAGAAAGGTGAAGTCGGGTCTATCCTGAATGAGGTGGATCCGGTACGTATTAATGCGTTGCAACGCGTAGCGATGGAGGAGTCCCGGTTGGGAATCCCTTTGTTGATAGCTCGCGATGTTATTCACGGGTTTAAAACCATTTTTCCCATCCCATTGGGACAAGCGGCTTCGTTCAATCCGCAGATTGCGAAAGACGGTGCACGGGTAGCGGCTATTGAGGCTTCTTCCGTAGGTATCCGTTGGACTTTTGCACCGATGATCGACATTGCCCGTGATCCTCGCTGGGGGCGCATTGCCGAAGGATGTGGTGAAGACACTTACCTGACTTCTGTAATGGGAGCTGCCATGGTAGAAGGTTTTCAGGGAGATTCTTTGAATAGTCCCACTTCCATAGCTGCCTGTCCTAAACATTTTGTGGGCTATGGTGCAGCTGAAGGCGGACGTGACTATAATTCGACATTTATTCCTGAACGTCGCCTGCGTAATGTTTACTTGCCACCGTTTGAAGCGGCAACGAAAGCGGGTGCAGCTACGTTTATGACTTCCTTTAATGATAATGATGGGATACCCTCTACCGGAAATGCTTTCATATTGAAAGATGTGCTTCGTGGCGAGTGGGGATTTGATGGTTTGGTAGTGACAGACTGGGCTTCTGCCAGCGAAATGATAAGTCATGGTTTTGCTGCCGATTCTAAAGAGGTAGCCATGAAATCAGTGAATGCTGGGGTGGATATGGAAATGGTAAGTTATACCTTTGTAAAAGAATTGCCTGCATTGATAAAAGAAGGAAAGGTGAAAGAAAGCACCATTGATGAAGCCGTTCGTAATATATTGCGCGTCAAGTATCGTCTGGGATTGTTTGATGTTCCTTATGTAGATGAAAAGCAACCCTCTGTCATGTATGATCCTTCTCATCTGAAAGTAGCTAAGCAGGCTGCTGTAGAATCGGCTATCCTGTTGAAGAATGATAAAGAAGTACTGCCGTTACAGGAGTCTCTGAAAACCATTGCTGTGGTAGGACCTATGGCCAATGCGCCTTATGAACAATTGGGTACCTGGATCTTTGATGGTGAGAAAGCTCATACTCAGACACCACTGAATGCTATTAAGGAAATAGTTGGCGACAAAGTACAGGTGATTTATGAACCCGGATTAGCTTATAGCCGTGAGAAAAATCCGGCAGGCGTAGCAAAAGCTGCTGCTGTTGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAAGCCATTCTTTCGGGTGAAGCACACTGTCTGGCAGATTTGAATCTTCAGGGTGATCAAAGTGCTTTGATTACGGCTTTGGCTAAGACAGGTAAACCTGTAGTAACCATTGTGATGGCAGGTCGTCCGTTGACTATCGGTCAGGAAGTGGAAGAATCAACAGCTGTTCTTTATTCATTCCATCCGGGTACGATGGGTGGACCGGCATTGGCCGATCTGCTGTGGGGTAAGGCGGTTCCAAGTGGAAAAACACCGGTTACTTTCCCGAAGATGGTAGGACAAATTCCGGTATATTATGCTCATAACAATACCGGGCGGCCGGCTACACGTAATGAGGTGTTGCTGGATGATATTGCTGTTGAGGCTGGACAAACTTCATTGGGATGTACTTCTTTCTATATGGATGCCGGTTTTGATCCTTTATTCCCATTTGGCTATGGCTTGTCGTATACAACGTTCAAGTATAGTAATGTCAAACTTTCATCAGCGTCATTGAAGAAAGATGATGTATTGACTGTGACATTTGATCTGGAAAATACAGGTAAATATAAGGGGACGGAAGTTGCTCAATTGTATATACAAGATAAGGTTGGTTCTGTAACTCGTCCGGTGAAAGAACTGAAACGTTTTACTCGGGTAACCTTGAAACCGGGCGAGAAAAAGAATGTTTCGTTTGAACTACCCGTTAGTGAACTTGCATTTTGGAACATCGATATGGTGAAAGTTGTGGAACCCGGAGACTTTGGACTTTGGGTGGCAACAGACAGCCAATCGGGAGAAGAAGTTTTCTTTAAGGTGGTAGAT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,102 total letters Query= CMR200122.116 Length=2598 Score E Sequences producing significant alignments: (Bits) Value wt 3947 0.0 08E_E8_CMR200122.116_E08.seq 401 2e-114 11E_E8_CMR200122.116_E08.seq 22.9 1.6 T Q V A N K G S D A A T E K K V E S L L S R M T L E E K I G Query_1 202 ACACAAGTGGCAAATAAAGGTAGCGATGCGGCAACCGAGAAAAAAGTAGAGTCTCTTTTATCCAGAATGACCCTTGAAGAGAAAATCGGT 291 2 1 .......................................................................................... 90 Q M N Q I T S Y G N I E D M S S L I K K G E V G S I L N E V Query_1 292 CAGATGAACCAGATTACCTCTTACGGGAATATTGAGGATATGAGTAGTTTAATTAAGAAAGGTGAAGTCGGGTCTATCCTGAATGAGGTG 381 2 91 .......................................................................................... 180 D P V R I N A L Q R V A M E E S R L G I P L L I A R D V I H Query_1 382 GATCCGGTACGTATTAATGCGTTGCAACGCGTAGCGATGGAGGAGTCCCGGTTGGGAATCCCTTTGTTGATAGCTCGCGATGTTATTCAC 471 2 181 .......................................................................................... 270 2 210 ............ 199 G F K T I F P I P L G Q A A S F N P Q I A K D G A R V A A I Query_1 472 GGGTTTAAAACCATTTTTCCCATCCCATTGGGACAAGCGGCTTCGTTCAATCCGCAGATTGCGAAAGACGGTGCACGGGTAGCGGCTATT 561 2 271 .......................................................................................... 360 E A S S V G I R W T F A P M I D I A R D P R W G R I A E G C Query_1 562 GAGGCTTCTTCCGTAGGTATCCGTTGGACTTTTGCACCGATGATCGACATTGCCCGTGATCCTCGCTGGGGGCGCATTGCCGAAGGATGT 651 2 361 .......................................................................................... 450 G E D T Y L T S V M G A A M V E G F Q G D S L N S P T S I A Query_1 652 GGTGAAGACACTTACCTGACTTCTGTAATGGGAGCTGCCATGGTAGAAGGTTTTCAGGGAGATTCTTTGAATAGTCCCACTTCCATAGCT 741 2 451 .......................................................................................... 540 A C P K H F V G Y G A A E G G R D Y N S T F I P E R R L R N Query_1 742 GCCTGTCCTAAACATTTTGTGGGCTATGGTGCAGCTGAAGGCGGACGTGACTATAATTCGACATTTATTCCTGAACGTCGCCTGCGTAAT 831 2 541 .......................................................................................... 630 V Y L P P F E A A T K A G A A T F M T S W N D N D G I P Query_1 832 GTTTACTTGCCACCGTTTGAAGCGGCAACGAAAGCGGGTGCAGCTACGTTTATGAC-TTC-CTGGA-ATGATAA-TGATGGGA-TACCC- 915 2 631 ........................................................-...-..TT.-.......-........-.....- 714 0 86 .......N...T....CT......CG........A.....C 126 1 555 .....-...-.... 566 S T G N A F I L K D V L R G E W G F D G L V V T D W A S A Query_1 916 TCTACCGGAAATGCTTTCATATTGAAAGATGTGCTTC-GTGGCGA-GTGGGGATTTGATGGTTTGGTAGTGACAGACTGGGCTTCTGCCA 1003 2 715 .....................................-.......-............................................ 802 0 127 ........G.......NT..T....NNA....N.A..C...A...N.C.......C........N..T.....G...............T 216 S E M I S H G F A A D S K E V A M K S V N A G V D M E M V S Query_1 1004 GCGAAATGATAAGTCATGGTTTTGCTGCCGATTCTAAAGAGGTAGCCATGAAATCAGTGAATGCTGGGGTGGATATGGAAATGGTAAGTT 1093 2 803 .......................................................................................... 892 0 217 ......N...N..C...........C....G...AN....A..GC.A........T........A..A..A..............G.... 306 Y T F V K E L P A L I K E G K V K E S T I D E A V R N I L R Query_1 1094 ATACCTTTGTAAAAGAATTGCCTGCATTGATAAAAGAAGGAAAGGTGAAAGAAAGCACCATTGATGAAGCCGTTCGTAATATATTGCGCG 1183 2 893 .......................................................................................... 982 0 307 .C..T.....G..G...C....N.A...NG.G..N..G.......G...G........T..CN....G..T....N...N.NT.....NA 396 V K Y R L G L F D V P Y V D E K Q P S V M Y D P S H L K V A Query_1 1184 TCAAGTATCGTCTGGGATTGTTTGATGTTCCTTATGTAGATGAAAAGCAACCCTCTGTCATGTATGATCCTTCTCATCTGAAAGTAGCTA 1273 2 983 .......................................................................................... 1072 0 397 .A.........T.A............ACA........N......C.A...A.A..........N..CN.....N...N..G...C.A... 486 K Q A A V E S A I L L K N D K E V L P L Q E S L K T I A V Query_1 1274 AGCAGGCTGCTGTAGAATCGGCTATCCTGTTGAAGAATGATAAA-GAAGTACTGCCGTTACAGGAGTCTCTGAAAAC-CATTGC-TGTGG 1360 2 1073 ............................................-................................-......-..... 1159 0 487 ...NA..CN....T.....N.....T.N.N....T........NC.....GT.N..N.......CA..NG.......TGT....ANAN.. 576 V G P M A N A P Y E Q L G T W I F D G E K A H T Q T P L Query_1 1361 TAGGACCTATGGCCAATGC-GCCTTATGAACA-ATTGGGTAC-CTGGATCTTTGATGGTGAGAAAGC-TCATACTCAGACACCACTGAA- 1445 2 1160 ...................-............-.........-........................-.....................- 1244 0 577 .......N.....TN.N.NTA..N........CT..AN.N..TT..N..A........A.....N..T.NG....TNN..C..GT...NC 666 N A I K E I V G D K V Q V I Y E P G L A Y S R E K N P A G V Query_1 1446 TGCTATTAAGGAAATAGTTGGC-GACAAAGTACAGGTGATTTATGAACC-CGGATTAGCTTATAGCCGTGAGAAAAATCCGGCAGGCGTA 1533 2 1245 ......................-..........................-........................................ 1332 0 667 NAN......A.....GN..N..TA.T..NA.NNCN.........N.N..G....C.. 723 A K A A A V A A R A D V I L A F V G E E A I L S G E A H C L Query_1 1534 GCAAAAGCTGCTGCTGTTGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAAGCCATTCTTTCGGGTGAAGCACACTGTCTG 1623 2 1333 .......................................................................................... 1422 2 1363 ............ 1352 A D L N L Q G D Q S A L I T A L A K T G K P V V T I V M A G Query_1 1624 GCAGATTTGAATCTTCAGGGTGATCAAAGTGCTTTGATTACGGCTTTGGCTAAGACAGGTAAACCTGTAGTAACCATTGTGATGGCAGGT 1713 2 1423 .......................................................................................... 1512 R P L T I G Q E V E E S T A V L Y S F H P G T M G G P A L A Query_1 1714 CGTCCGTTGACTATCGGTCAGGAAGTGGAAGAATCAACAGCTGTTCTTTATTCATTCCATCCGGGTACGATGGGTGGACCGGCATTGGCC 1803 2 1513 .......................................................................................... 1602 D L L W G K A V P S G K T P V T F P K M V G Q I P V Y Y A H Query_1 1804 GATCTGCTGTGGGGTAAGGCGGTTCCAAGTGGAAAAACACCGGTTACTTTCCCGAAGATGGTAGGACAAATTCCGGTATATTATGCTCAT 1893 2 1603 .......................................................................................... 1692 N N T G R P A T R N E V L L D D I A V E A G Q T S L G C T S Query_1 1894 AACAATACCGGGCGGCCGGCTACACGTAATGAGGTGTTGCTGGATGATATTGCTGTTGAGGCTGGACAAACTTCATTGGGATGTACTTCT 1983 2 1693 .......................................................................................... 1782 F Y M D A G F D P L F P F G Y G L S Y T T F K Y S N V K L S Query_1 1984 TTCTATATGGATGCCGGTTTTGATCCTTTATTCCCATTTGGCTATGGCTTGTCGTATACAACGTTCAAGTATAGTAATGTCAAACTTTCA 2073 2 1783 .......................................................................................... 1872 S A S L K K D D V L T V T F D L E N T G K Y K G T E V A Q L Query_1 2074 TCAGCGTCATTGAAGAAAGATGATGTATTGACTGTGACATTTGATCTGGAAAATACAGGTAAATATAAGGGGACGGAAGTTGCTCAATTG 2163 2 1873 .......................................................................................... 1962 2 1971 ... 1969 Y I Q D K V G S V T R P V K E L K R F T R V T L K P G E K K Query_1 2164 TATATACAAGATAAGGTTGGTTCTGTAACTCGTCCGGTGAAAGAACTGAAACGTTTTACTCGGGTAACCTTGAAACCGGGCGAGAAAAAG 2253 2 1963 .......................................................................................... 2052 2 1968 ......... 1960 N V S F E L P V S E L A F W N I D M V K V V E P G D F G L W Query_1 2254 AATGTTTCGTTTGAACTACCCGTTAGTGAACTTGCATTTTGGAACATCGATATGGTGAAAGTTGTGGAACCCGGAGACTTTGGACTTTGG 2343 2 2053 .......................................................................................... 2142 V A T D S Q S G E E V F F K V V D Query_1 2344 GTGGCAACAGACAGCCAATCGGGAGAAGAAGTTTTCTTTAAGGTGGTAGAT 2394 2 2143 ................................................... 2193 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 13036631 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 5,102 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200130.105 (CMR200130.32-851.P(851).E440A.pMCSG68)
SDSNVDFNKDWKFVLKDSAHYSYTSYVPGDEWKKVNLPHDWSVGLPYDSISGEGCVAFLQGGIGWYSKSFPTTISANQKCYIVFDGVYNNSEYWINGKKLGYHLSGYAPFYFDVTDYLNPNEDNRMTVRVDHSHYADSRWYTGSGIYRDVKMIVTDRLHIPVWGTFVTTPVVTDKYAKVNNQITVRNSYSEPRTAVVEIVYKDNKGNIAAFEVFSIKLNAGEEKIIDIVSEIKQPDLWSVEIPVLYTAETRIKNGDEVISENTVRFGIRTFHFDADKGFFLNGKNMKIKGVCLHHDAGIVGTAMIRDVWYRRLKTLKEGGCNAIRLSHNPGADEFLSLCDEIGLLVQEEFFDEWDYPKDKRLNMKETVEDYPTHGYCEHFQEWAERDLKNVMRRSRNHACIFQWSIGNEIEWTYTGCREATGFFGADSNGNYFWNQPPYSKEKIREMWKIQPKQAYDIGRTAQKLAAWTRQMDTTRVVTANCILPSISFETGYIDALDVAGFSYRRVMYDYAKKNYPDKPIMGTENLGQWHEWKAVIERDFVPGMFIWTGVDYLGESGSRLSRWPQKSIGCGLLDMCGYVKPSYDMMKSLWTDKPFIAIYSQTPDKSSYLQVKDGFTDKKGHEWDRRLWVWDDVNSHWNYQKGDSVIVEIYSNCDEVELFVNGKSMGKKYIDDFEDHIYKWAVQYKPGTITAKGKNKLGNTTTAIRTSGKEHSILLAVDKQSIAANGKDVLHVTAQLTDKKGNPVKTTEQMLKFNIDGEYRLLGIDNGNVKNVSPYQSKEIMTYQGRCMLMLQSTEKTSVLNISAETSELQSNKLTINIK
TCTGATTCAAATGTTGATTTCAATAAAGATTGGAAATTCGTACTGAAAGATTCTGCTCATTATTCATATACTTCTTATGTCCCTGGTGATGAATGGAAGAAAGTGAACCTGCCACACGACTGGAGTGTTGGTCTGCCTTACGACTCCATCTCTGGCGAAGGGTGTGTAGCTTTCCTTCAGGGAGGAATAGGATGGTATAGCAAATCATTTCCCACAACAATCAGCGCAAATCAGAAATGCTATATAGTGTTCGATGGAGTATATAATAATTCTGAGTATTGGATAAATGGCAAAAAACTTGGATATCATCTTTCGGGATATGCTCCTTTTTATTTTGATGTCACAGACTATCTCAATCCCAATGAGGATAACCGCATGACTGTAAGGGTCGACCACAGCCATTATGCCGACAGCAGATGGTACACCGGTTCAGGTATATACAGGGATGTGAAAATGATTGTAACCGACAGACTGCATATTCCGGTTTGGGGAACATTTGTCACTACTCCCGTGGTTACTGATAAATATGCTAAAGTAAACAACCAAATTACCGTGCGCAACAGTTACTCTGAACCCAGAACAGCTGTTGTTGAGATAGTGTATAAAGATAATAAAGGCAATATCGCAGCCTTTGAGGTCTTCAGTATAAAACTGAATGCTGGTGAGGAGAAAATTATCGACATCGTATCGGAGATAAAACAGCCGGATTTGTGGAGCGTCGAGATACCAGTCCTCTATACAGCCGAGACCCGTATTAAGAATGGCGATGAAGTCATTTCTGAAAACACTGTCAGGTTCGGTATACGAACATTCCACTTTGATGCAGACAAAGGTTTCTTCCTTAACGGAAAAAATATGAAGATAAAAGGAGTATGCCTGCATCATGATGCCGGTATAGTTGGCACAGCAATGATACGCGATGTGTGGTACCGACGTCTGAAAACCCTTAAGGAAGGAGGATGTAACGCCATCCGCCTTTCGCACAATCCGGGAGCGGATGAGTTTCTGTCTTTGTGCGATGAGATAGGTCTTCTGGTCCAGGAAGAGTTCTTCGATGAGTGGGATTATCCCAAAGATAAAAGGCTCAATATGAAGGAAACGGTAGAAGACTATCCTACTCATGGTTATTGTGAGCATTTCCAGGAATGGGCTGAAAGGGATTTGAAAAACGTAATGAGGAGAAGCCGTAATCATGCCTGTATCTTCCAGTGGAGTATAGGTAATGAAATAGAATGGACTTATACCGGATGCCGTGAGGCAACAGGTTTCTTTGGAGCCGATTCCAACGGTAATTACTTCTGGAACCAGCCTCCATACTCTAAAGAAAAAATCAGAGAAATGTGGAAAATCCAGCCTAAACAAGCATACGACATTGGTCGTACAGCGCAAAAATTAGCAGCATGGACACGCCAGATGGATACTACACGAGTGGTTACCGCCAACTGCATCCTGCCTTCCATAAGTTTTGAGACAGGATATATCGATGCACTTGATGTGGCTGGTTTCAGCTACAGACGCGTGATGTATGATTATGCTAAGAAGAATTATCCTGACAAACCTATAATGGGTACAGAAAATCTTGGTCAGTGGCACGAATGGAAGGCGGTGATTGAAAGAGATTTCGTTCCGGGTATGTTTATATGGACAGGAGTCGATTATCTGGGAGAAAGTGGAAGCCGCCTTTCAAGATGGCCTCAAAAGTCAATAGGATGTGGTCTCCTGGATATGTGCGGCTATGTGAAGCCTTCGTACGACATGATGAAATCATTGTGGACTGACAAGCCTTTTATTGCTATATATTCACAGACTCCAGACAAATCTTCGTATCTCCAGGTAAAAGATGGCTTTACTGATAAGAAAGGACATGAATGGGATAGAAGATTATGGGTTTGGGATGATGTAAACTCTCACTGGAATTATCAGAAAGGTGACTCGGTAATAGTAGAAATATATTCCAATTGTGATGAAGTGGAACTTTTCGTTAACGGCAAGTCGATGGGAAAGAAGTATATAGACGATTTTGAGGATCATATCTATAAATGGGCAGTTCAGTACAAGCCTGGCACTATTACCGCAAAAGGAAAAAATAAGTTAGGTAATACCACTACAGCTATAAGGACTTCAGGCAAAGAACATTCGATATTGCTAGCGGTTGACAAACAAAGTATCGCAGCAAATGGAAAGGATGTTCTGCATGTCACAGCCCAGCTTACAGACAAAAAAGGTAATCCTGTAAAGACAACAGAACAGATGCTTAAGTTCAACATCGATGGAGAGTACCGTCTGTTGGGTATAGACAATGGAAATGTAAAGAACGTATCTCCATATCAAAGCAAGGAGATTATGACATATCAGGGAAGATGTATGCTGATGCTTCAGTCAACAGAAAAAACATCGGTACTGAATATCAGTGCAGAAACAAGTGAATTACAGTCGAATAAACTAACAATTAATATAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,404 total letters Query= CMR200130.105 Length=2865 Score E Sequences producing significant alignments: (Bits) Value wt 4428 0.0 08F_F8_CMR200130.105_F08.seq 933 0.0 S D S N V D F N K D W K F V L K D S A H Y S Y T S Y V P G D Query_1 202 TCTGATTCAAATGTTGATTTCAATAAAGATTGGAAATTCGTACTGAAAGATTCTGCTCATTATTCATATACTTCTTATGTCCCTGGTGAT 291 2 1 .......................................................................................... 90 2 2012 ........... 2002 E W K K V N L P H D W S V G L P Y D S I S G E G C V A F L Q Query_1 292 GAATGGAAGAAAGTGAACCTGCCACACGACTGGAGTGTTGGTCTGCCTTACGACTCCATCTCTGGCGAAGGGTGTGTAGCTTTCCTTCAG 381 2 91 .......................................................................................... 180 G G I G W Y S K S F P T T I S A N Q K C Y I V F D G V Y N N Query_1 382 GGAGGAATAGGATGGTATAGCAAATCATTTCCCACAACAATCAGCGCAAATCAGAAATGCTATATAGTGTTCGATGGAGTATATAATAAT 471 2 181 .......................................................................................... 270 S E Y W I N G K K L G Y H L S G Y A P F Y F D V T D Y L N P Query_1 472 TCTGAGTATTGGATAAATGGCAAAAAACTTGGATATCATCTTTCGGGATATGCTCCTTTTTATTTTGATGTCACAGACTATCTCAATCCC 561 2 271 .......................................................................................... 360 N E D N R M T V R V D H S H Y A D S R W Y T G S G I Y R D V Query_1 562 AATGAGGATAACCGCATGACTGTAAGGGTCGACCACAGCCATTATGCCGACAGCAGATGGTACACCGGTTCAGGTATATACAGGGATGTG 651 2 361 .......................................................................................... 450 K M I V T D R L H I P V W G T F V T T P V V T D K Y A K V N Query_1 652 AAAATGATTGTAACCGACAGACTGCATATTCCGGTTTGGGGAACATTTGTCACTACTCCCGTGGTTACTGATAAATATGCTAAAGTAAAC 741 2 451 .......................................................................................... 540 N Q I T V R N S Y S E P R T A V V E I V Y K D N K G N I A A Query_1 742 AACCAAATTACCGTGCGCAACAGTTACTCTGAACCCAGAACAGCTGTTGTTGAGATAGTGTATAAAGATAATAAAGGCAATATCGCAGCC 831 2 541 .......................................................................................... 630 F E V F S I K L N A G E E K I I D I V S E I K Q P D L W S V Query_1 832 TTTGAGGTCTTCAGTATAAAACTGAATGCTGGTGAGGAGAAAATTATCGACATCGTATCGGAGATAAAACAGCCGGATTTGTGGAGCGTC 921 2 631 .......................................................................................... 720 E I P V L Y T A E T R I K N G D E V I S E N T V R F G I R T Query_1 922 GAGATACCAGTCCTCTATACAGCCGAGACCCGTATTAAGAATGGCGATGAAGTCATTTCTGAAAACACTGTCAGGTTCGGTATACGAACA 1011 2 721 .......................................................................................... 810 F H F D A D K G F F L N G K N M K I K G V C L H H D A G I V Query_1 1012 TTCCACTTTGATGCAGACAAAGGTTTCTTCCTTAACGGAAAAAATATGAAGATAAAAGGAGTATGCCTGCATCATGATGCCGGTATAGTT 1101 2 811 .......................................................................................... 900 2 897 ...A..G............A..C... 872 2 1073 ........... 1083 G T A M I R D V W Y R R L K T L K E G G C N A I R L S H N P Query_1 1102 GGCACAGCAATGATACGCGATGTGTGGTACCGACGTCTGAAAACCCTTAAGGAAGGAGGATGTAACGCCATCCGCCTTTCGCACAATCCG 1191 2 901 .......................................................................................... 990 G A D E F L S L C D E I G L L V Q E E F F D E W D Y P K D K Query_1 1192 GGAGCGGATGAGTTTCTGTCTTTGTGCGATGAGATAGGTCTTCTGGTCCAGGAAGAGTTCTTCGATGAGTGGGATTATCCCAAAGATAAA 1281 2 991 .......................................................................................... 1080 R L N M K E T V E D Y P T H G Y C E H F Q E W A E R D L K N Query_1 1282 AGGCTCAATATGAAGGAAACGGTAGAAGACTATCCTACTCATGGTTATTGTGAGCATTTCCAGGAATGGGCTGAAAGGGATTTGAAAAAC 1371 2 1081 .......................................................................................... 1170 V M R R S R N H A C I F Q W S I G N A I E W T Y T G C R E A Query_1 1372 GTAATGAGGAGAAGCCGTAATCATGCCTGTATCTTCCAGTGGAGTATAGGTAATGCTATAGAATGGACTTATACCGGATGCCGTGAGGCA 1461 2 1171 .......................................................AA................................. 1260 T G F F G A D S N G N Y F W N Q P P Y S K E K I R E M W K I Query_1 1462 ACAGGTTTCTTTGGAGCCGATTCCAACGGTAATTACTTCTGGAACCAGCCTCCATACTCTAAAGAAAAAATCAGAGAAATGTGGAAAATC 1551 2 1261 .......................................................................................... 1350 Q P K Q A Y D I G R T A Q K L A A W T R Q M D T T R V V T A Query_1 1552 CAGCCTAAACAAGCATACGACATTGGTCGTACAGCGCAAAAATTAGCAGCATGGACACGCCAGATGGATACTACACGAGTGGTTACCGCC 1641 2 1351 .......................................................................................... 1440 0 28 .........N..................... 58 N C I L P S I S F E T G Y I D A L D V A G F S Y R R V M Y Query_1 1642 AACTGCATCCTGCCTTCCATAAGTTTTGAGACAGGATATATCGATGCACTTGATGTGGCTGGTTT-CAGCTACAGACGCG-TGATGTATG 1729 2 1441 .................................................................-..............-......... 1528 0 59 .........................................................N.......T....GCT.......C......... 148 D Y A K K N Y P D K P I M G T E N L G Q W H E W K A V I E R Query_1 1730 ATTATGCTAAGAAGAATTATCCTGACAAACCTATAATGGGTACAGAAAATCTTGGTCAGTGGCACGAATGGAAGGCGGTGATTGAAAGAG 1819 2 1529 .......................................................................................... 1618 0 149 .......................................................................................... 238 D F V P G M F I W T G V D Y L G E S G S R L S R W P Q K S I Query_1 1820 ATTTCGTTCCGGGTATGTTTATATGGACAGGAGTCGATTATCTGGGAGAAAGTGGAAGCCGCCTTTCAAGATGGCCTCAAAAGTCAATAG 1909 2 1619 .......................................................................................... 1708 0 239 ......N......C...................N..................................N..........N.......... 328 G C G L L D M C G Y V K P S Y D M M K S L W T D K P F I A I Query_1 1910 GATGTGGTCTCCTGGATATGTGCGGCTATGTGAAGCCTTCGTACGACATGATGAAATCATTGTGGACTGACAAGCC-TTTTATTGCTATA 1998 2 1709 ............................................................................-............. 1797 0 329 ....C.......................................................................T............. 418 Y S Q T P D K S S Y L Q V K D G F T D K K G H E W D R R L Query_1 1999 TATTCACAGACTCCAGACAAATCT-TCGTATCTCCAGGTAAAAGATGGCTTTACTGATAAGAAAGGACATG-AATGGGATAGAAGATTAT 2086 2 1798 ........................-..............................................-.................. 1885 0 419 ........................C..N....N..N................N.....N.T..........TN....N......N..... 508 W V W D D V N S H W N Y Q K G D S V I V E I Y S N C D E V Query_1 2087 GGGTTTGGGATGATGTAAACTCTCACTGGAATTAT--CAGAAAGGTGACTCGGT-AATAGTAG-AAATATATTCCAATTGTGATGAAGTG 2172 2 1886 ...................................--.................-........-.......................... 1971 0 509 ...C...........N.................NNTC..N.NNN.....N....C.N......N......N................C.. 598 E L F V N G K S M G K K Y I D D F E D H I Y K W A V Q Y Query_1 2173 GAACTTTTCGTTAA-CGGCAAGTCGATGGG-AAAG-AAGTATATAGACGATTTTGAGGATCATATC-TATAAATGGGCAGTTCAGTACAA 2258 2 1972 ..............-...............-....-..............................-....................... 2057 2 76 ...-........ 66 0 599 N........T....C.N..........N.NC....C.NN....N.N..N.................T...N........N....N..NN. 688 K P G T I T A K G K N K L G N T T T A I R T S G K E H S I L Query_1 2259 GCCTGGCACTAT-TACCGCAAAAGGAAAAAATAAGTTAGGTAATACCACTACAGCTATAAGGACTTCAGGCAAAGAACATTCGATATTGC 2347 2 2058 ............-............................................................................. 2146 0 689 N.N....T....N.N......... 712 L A V D K Q S I A A N G K D V L H V T A Q L T D K K G N P V Query_1 2348 TAGCGGTTGACAAACAAAGTATCGCAGCAAATGGAAAGGATGTTCTGCATGTCACAGCCCAGCTTACAGACAAAAAAGGTAATCCTGTAA 2437 2 2147 .......................................................................................... 2236 K T T E Q M L K F N I D G E Y R L L G I D N G N V K N V S P Query_1 2438 AGACAACAGAACAGATGCTTAAGTTCAACATCGATGGAGAGTACCGTCTGTTGGGTATAGACAATGGAAATGTAAAGAACGTATCTCCAT 2527 2 2237 .......................................................................................... 2326 Y Q S K E I M T Y Q G R C M L M L Q S T E K T S V L N I S A Query_1 2528 ATCAAAGCAAGGAGATTATGACATATCAGGGAAGATGTATGCTGATGCTTCAGTCAACAGAAAAAACATCGGTACTGAATATCAGTGCAG 2617 2 2327 .......................................................................................... 2416 E T S E L Q S N K L T I N I K Query_1 2618 AAACAAGTGAATTACAGTCGAATAAACTAACAATTAATATAAAA 2661 2 2417 ............................................ 2460 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 15231450 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 5,404 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200130.107 (CMR200130.32-851.P(851).Y535A.pMCSG68)
SDSNVDFNKDWKFVLKDSAHYSYTSYVPGDEWKKVNLPHDWSVGLPYDSISGEGCVAFLQGGIGWYSKSFPTTISANQKCYIVFDGVYNNSEYWINGKKLGYHLSGYAPFYFDVTDYLNPNEDNRMTVRVDHSHYADSRWYTGSGIYRDVKMIVTDRLHIPVWGTFVTTPVVTDKYAKVNNQITVRNSYSEPRTAVVEIVYKDNKGNIAAFEVFSIKLNAGEEKIIDIVSEIKQPDLWSVEIPVLYTAETRIKNGDEVISENTVRFGIRTFHFDADKGFFLNGKNMKIKGVCLHHDAGIVGTAMIRDVWYRRLKTLKEGGCNAIRLSHNPGADEFLSLCDEIGLLVQEEFFDEWDYPKDKRLNMKETVEDYPTHGYCEHFQEWAERDLKNVMRRSRNHACIFQWSIGNEIEWTYTGCREATGFFGADSNGNYFWNQPPYSKEKIREMWKIQPKQAYDIGRTAQKLAAWTRQMDTTRVVTANCILPSISFETGYIDALDVAGFSYRRVMYDYAKKNYPDKPIMGTENLGQWHEWKAVIERDFVPGMFIWTGVDYLGESGSRLSRWPQKSIGCGLLDMCGYVKPSYDMMKSLWTDKPFIAIYSQTPDKSSYLQVKDGFTDKKGHEWDRRLWVWDDVNSHWNYQKGDSVIVEIYSNCDEVELFVNGKSMGKKYIDDFEDHIYKWAVQYKPGTITAKGKNKLGNTTTAIRTSGKEHSILLAVDKQSIAANGKDVLHVTAQLTDKKGNPVKTTEQMLKFNIDGEYRLLGIDNGNVKNVSPYQSKEIMTYQGRCMLMLQSTEKTSVLNISAETSELQSNKLTINIK
TCTGATTCAAATGTTGATTTCAATAAAGATTGGAAATTCGTACTGAAAGATTCTGCTCATTATTCATATACTTCTTATGTCCCTGGTGATGAATGGAAGAAAGTGAACCTGCCACACGACTGGAGTGTTGGTCTGCCTTACGACTCCATCTCTGGCGAAGGGTGTGTAGCTTTCCTTCAGGGAGGAATAGGATGGTATAGCAAATCATTTCCCACAACAATCAGCGCAAATCAGAAATGCTATATAGTGTTCGATGGAGTATATAATAATTCTGAGTATTGGATAAATGGCAAAAAACTTGGATATCATCTTTCGGGATATGCTCCTTTTTATTTTGATGTCACAGACTATCTCAATCCCAATGAGGATAACCGCATGACTGTAAGGGTCGACCACAGCCATTATGCCGACAGCAGATGGTACACCGGTTCAGGTATATACAGGGATGTGAAAATGATTGTAACCGACAGACTGCATATTCCGGTTTGGGGAACATTTGTCACTACTCCCGTGGTTACTGATAAATATGCTAAAGTAAACAACCAAATTACCGTGCGCAACAGTTACTCTGAACCCAGAACAGCTGTTGTTGAGATAGTGTATAAAGATAATAAAGGCAATATCGCAGCCTTTGAGGTCTTCAGTATAAAACTGAATGCTGGTGAGGAGAAAATTATCGACATCGTATCGGAGATAAAACAGCCGGATTTGTGGAGCGTCGAGATACCAGTCCTCTATACAGCCGAGACCCGTATTAAGAATGGCGATGAAGTCATTTCTGAAAACACTGTCAGGTTCGGTATACGAACATTCCACTTTGATGCAGACAAAGGTTTCTTCCTTAACGGAAAAAATATGAAGATAAAAGGAGTATGCCTGCATCATGATGCCGGTATAGTTGGCACAGCAATGATACGCGATGTGTGGTACCGACGTCTGAAAACCCTTAAGGAAGGAGGATGTAACGCCATCCGCCTTTCGCACAATCCGGGAGCGGATGAGTTTCTGTCTTTGTGCGATGAGATAGGTCTTCTGGTCCAGGAAGAGTTCTTCGATGAGTGGGATTATCCCAAAGATAAAAGGCTCAATATGAAGGAAACGGTAGAAGACTATCCTACTCATGGTTATTGTGAGCATTTCCAGGAATGGGCTGAAAGGGATTTGAAAAACGTAATGAGGAGAAGCCGTAATCATGCCTGTATCTTCCAGTGGAGTATAGGTAATGAAATAGAATGGACTTATACCGGATGCCGTGAGGCAACAGGTTTCTTTGGAGCCGATTCCAACGGTAATTACTTCTGGAACCAGCCTCCATACTCTAAAGAAAAAATCAGAGAAATGTGGAAAATCCAGCCTAAACAAGCATACGACATTGGTCGTACAGCGCAAAAATTAGCAGCATGGACACGCCAGATGGATACTACACGAGTGGTTACCGCCAACTGCATCCTGCCTTCCATAAGTTTTGAGACAGGATATATCGATGCACTTGATGTGGCTGGTTTCAGCTACAGACGCGTGATGTATGATTATGCTAAGAAGAATTATCCTGACAAACCTATAATGGGTACAGAAAATCTTGGTCAGTGGCACGAATGGAAGGCGGTGATTGAAAGAGATTTCGTTCCGGGTATGTTTATATGGACAGGAGTCGATTATCTGGGAGAAAGTGGAAGCCGCCTTTCAAGATGGCCTCAAAAGTCAATAGGATGTGGTCTCCTGGATATGTGCGGCTATGTGAAGCCTTCGTACGACATGATGAAATCATTGTGGACTGACAAGCCTTTTATTGCTATATATTCACAGACTCCAGACAAATCTTCGTATCTCCAGGTAAAAGATGGCTTTACTGATAAGAAAGGACATGAATGGGATAGAAGATTATGGGTTTGGGATGATGTAAACTCTCACTGGAATTATCAGAAAGGTGACTCGGTAATAGTAGAAATATATTCCAATTGTGATGAAGTGGAACTTTTCGTTAACGGCAAGTCGATGGGAAAGAAGTATATAGACGATTTTGAGGATCATATCTATAAATGGGCAGTTCAGTACAAGCCTGGCACTATTACCGCAAAAGGAAAAAATAAGTTAGGTAATACCACTACAGCTATAAGGACTTCAGGCAAAGAACATTCGATATTGCTAGCGGTTGACAAACAAAGTATCGCAGCAAATGGAAAGGATGTTCTGCATGTCACAGCCCAGCTTACAGACAAAAAAGGTAATCCTGTAAAGACAACAGAACAGATGCTTAAGTTCAACATCGATGGAGAGTACCGTCTGTTGGGTATAGACAATGGAAATGTAAAGAACGTATCTCCATATCAAAGCAAGGAGATTATGACATATCAGGGAAGATGTATGCTGATGCTTCAGTCAACAGAAAAAACATCGGTACTGAATATCAGTGCAGAAACAAGTGAATTACAGTCGAATAAACTAACAATTAATATAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,903 total letters Query= CMR200130.107 Length=2865 Score E Sequences producing significant alignments: (Bits) Value wt 4423 0.0 08G_G8_CMR200130.107_G08.seq 937 0.0 S D S N V D F N K D W K F V L K D S A H Y S Y T S Y V P G D Query_1 202 TCTGATTCAAATGTTGATTTCAATAAAGATTGGAAATTCGTACTGAAAGATTCTGCTCATTATTCATATACTTCTTATGTCCCTGGTGAT 291 2 1 .......................................................................................... 90 2 2012 ........... 2002 E W K K V N L P H D W S V G L P Y D S I S G E G C V A F L Q Query_1 292 GAATGGAAGAAAGTGAACCTGCCACACGACTGGAGTGTTGGTCTGCCTTACGACTCCATCTCTGGCGAAGGGTGTGTAGCTTTCCTTCAG 381 2 91 .......................................................................................... 180 G G I G W Y S K S F P T T I S A N Q K C Y I V F D G V Y N N Query_1 382 GGAGGAATAGGATGGTATAGCAAATCATTTCCCACAACAATCAGCGCAAATCAGAAATGCTATATAGTGTTCGATGGAGTATATAATAAT 471 2 181 .......................................................................................... 270 S E Y W I N G K K L G Y H L S G Y A P F Y F D V T D Y L N P Query_1 472 TCTGAGTATTGGATAAATGGCAAAAAACTTGGATATCATCTTTCGGGATATGCTCCTTTTTATTTTGATGTCACAGACTATCTCAATCCC 561 2 271 .......................................................................................... 360 N E D N R M T V R V D H S H Y A D S R W Y T G S G I Y R D V Query_1 562 AATGAGGATAACCGCATGACTGTAAGGGTCGACCACAGCCATTATGCCGACAGCAGATGGTACACCGGTTCAGGTATATACAGGGATGTG 651 2 361 .......................................................................................... 450 K M I V T D R L H I P V W G T F V T T P V V T D K Y A K V N Query_1 652 AAAATGATTGTAACCGACAGACTGCATATTCCGGTTTGGGGAACATTTGTCACTACTCCCGTGGTTACTGATAAATATGCTAAAGTAAAC 741 2 451 .......................................................................................... 540 N Q I T V R N S Y S E P R T A V V E I V Y K D N K G N I A A Query_1 742 AACCAAATTACCGTGCGCAACAGTTACTCTGAACCCAGAACAGCTGTTGTTGAGATAGTGTATAAAGATAATAAAGGCAATATCGCAGCC 831 2 541 .......................................................................................... 630 F E V F S I K L N A G E E K I I D I V S E I K Q P D L W S V Query_1 832 TTTGAGGTCTTCAGTATAAAACTGAATGCTGGTGAGGAGAAAATTATCGACATCGTATCGGAGATAAAACAGCCGGATTTGTGGAGCGTC 921 2 631 .......................................................................................... 720 E I P V L Y T A E T R I K N G D E V I S E N T V R F G I R T Query_1 922 GAGATACCAGTCCTCTATACAGCCGAGACCCGTATTAAGAATGGCGATGAAGTCATTTCTGAAAACACTGTCAGGTTCGGTATACGAACA 1011 2 721 .......................................................................................... 810 F H F D A D K G F F L N G K N M K I K G V C L H H D A G I V Query_1 1012 TTCCACTTTGATGCAGACAAAGGTTTCTTCCTTAACGGAAAAAATATGAAGATAAAAGGAGTATGCCTGCATCATGATGCCGGTATAGTT 1101 2 811 .......................................................................................... 900 2 897 ...A..G............A..C... 872 2 1073 ........... 1083 G T A M I R D V W Y R R L K T L K E G G C N A I R L S H N P Query_1 1102 GGCACAGCAATGATACGCGATGTGTGGTACCGACGTCTGAAAACCCTTAAGGAAGGAGGATGTAACGCCATCCGCCTTTCGCACAATCCG 1191 2 901 .......................................................................................... 990 G A D E F L S L C D E I G L L V Q E E F F D E W D Y P K D K Query_1 1192 GGAGCGGATGAGTTTCTGTCTTTGTGCGATGAGATAGGTCTTCTGGTCCAGGAAGAGTTCTTCGATGAGTGGGATTATCCCAAAGATAAA 1281 2 991 .......................................................................................... 1080 2 859 ........ 866 R L N M K E T V E D Y P T H G Y C E H F Q E W A E R D L K N Query_1 1282 AGGCTCAATATGAAGGAAACGGTAGAAGACTATCCTACTCATGGTTATTGTGAGCATTTCCAGGAATGGGCTGAAAGGGATTTGAAAAAC 1371 2 1081 .......................................................................................... 1170 2 867 ... 869 0 1403 ..G............ 1389 V M R R S R N H A C I F Q W S I G N E I E W T Y T G C R E A Query_1 1372 GTAATGAGGAGAAGCCGTAATCATGCCTGTATCTTCCAGTGGAGTATAGGTAATGAAATAGAATGGACTTATACCGGATGCCGTGAGGCA 1461 2 1171 .......................................................................................... 1260 T G F F G A D S N G N Y F W N Q P P Y S K E K I R E M W K I Query_1 1462 ACAGGTTTCTTTGGAGCCGATTCCAACGGTAATTACTTCTGGAACCAGCCTCCATACTCTAAAGAAAAAATCAGAGAAATGTGGAAAATC 1551 2 1261 .......................................................................................... 1350 Q P K Q A Y D I G R T A Q K L A A W T R Q M D T T R V V T Query_1 1552 CAGCCTAAACAAGCATACGACATTGGTCGTACAGCGCAAAAATTAGCAGCATGG-ACACGCCAGA-TGG-ATACTACACGAGTGGTTACC 1638 2 1351 ......................................................-..........-...-.................... 1437 0 6 ..A..A........T....N.NC..A...C.................... 55 A N C I L P S I S F E T G Y I D A L D V A G F S A R R V M Y Query_1 1639 GCCAACTGCATCCTGCCTTCCATAAGTTTTGAGACAGGATATATCGATGCACTTGATGTGGCTGGTTTCAGCGCTAGACGCGTGATGTAT 1728 2 1438 ........................................................................TAC............... 1527 0 56 .N.....N.C.TT..N.......................N................................NAC.......N....... 145 D Y A K K N Y P D K P I M G T E N L G Q W H E W K A V I E R Query_1 1729 GATTATGCTAAGAAGAATTATCCTGACAAACCTATAATGGGTACAGAAAATCTTGGTCAGTGGCACGAATGGAAGGCGGTGATTGAAAGA 1818 2 1528 .......................................................................................... 1617 0 146 .........NN..................................C.G.......................................... 235 D F V P G M F I W T G V D Y L G E S G S R L S R W P Q K S I Query_1 1819 GATTTCGTTCCGGGTATGTTTATATGGACAGGAGTCGATTATCTGGGAGAAAGTGGAAGCCGCCTTTCAAGATGGCCTCAAAAGTCAATA 1908 2 1618 .......................................................................................... 1707 0 236 ........N................................N..........................................N..... 325 G C G L L D M C G Y V K P S Y D M M K S L W T D K P F I A I Query_1 1909 GGATGTGGTCTCCTGGATATGTGCGGCTATGTGAAGCCTTCGTACGACATGATGAAATCATTGTGGACTGACAAGCCTTTTATTGCTATA 1998 2 1708 .......................................................................................... 1797 0 326 .............N...N..................................N..................................... 415 Y S Q T P D K S S Y L Q V K D G F T D K K G H E W D R R L Query_1 1999 TATTCACAGACTCCAGACAAATCTTCGTATCTCCAGGTAAAAGATGGCT-TTA-CTGATAAGAAAGGACATGAATGGGATAGAAGAT-TA 2085 2 1798 .................................................-...-.................................-.. 1884 0 416 ...........N........................N............C...C....N..N.........................CN. 505 W V W D D V N S H W N Y Q K G D S V I V E I Y S N C D E Query_1 2086 TGGGTTTGGGATGATGTAAACTCTCACTGGAATTATCA-GAAAGGTGACTCGGTAATAGTAGAA-ATATATTCC--AATTGTGATGAA-G 2170 2 1885 ......................................-.........................-.........--............-. 1969 0 506 ....N.............................N...CT...................N....C......N..CA.....A..N...N. 595 V E L F V N G K S M G K K Y I D D F E D H I Y K W A Query_1 2171 TGGAACTTT-TCG-TTAACGGCAAG-TCG-ATGGGAAA--GAA-GTATATA-GACGATT-TTGA-GGATCATATCTA--TAAATGGGC-A 2247 2 1970 .........-...-...........-...-........--...-.......-.......-....-............--.........-. 2046 2 76 ..--...-...... 66 0 596 ..N......N...NN........C.CN..A.N......AN...NN.N....G.N.C...N.N..G..........NNAT..N....N.N. 685 V Q Y K P G T I T A K G K N K L G N T T T A I R T S Query_1 2248 G-TTCAGTACAAGCCTGGCACTA-TTACCGC-AAAAGGAAAAAAT-AAGTT-AGGTA-ATA-CCACTACAG-CTAT-AAGGACTT-CAGG 2327 2 2047 .-.....................-.......-.............-.....-.....-...-.........-....-........-.... 2126 0 686 .T.....................T.......N.............N.....CN..N.N...C..C....N.N....A.......NC.CN. 775 G K E H S I L L A V D K Q S I A A N G K D V L H V T Query_1 2328 C---AAAGAACATT-CGATATTGCT-AGC-GGTTG-ACAA---ACAAAGTATCGCAGCAAATGG-AAAGGAT-GTTCTGCATGTCACAGC 2405 2 2127 .---..........-..........-...-.....-....---.....................-.......-................. 2204 0 776 .CNA..NN.N....C.N........C..NG.....C....ANC.N..N.A.N....N..N...NT.......G.N...N..N........ 865 A Q L T D K K G N P V K T T E Q M L K F N I D G E Y R L L G Query_1 2406 CCAG-CTTACAGA-CAAAAAAGGTAATCCTGTAAAGACAACAGAACAGATGCTTAAGTTCAACATCGATGGAGAGTACCGTCTGTTGGGT 2493 2 2205 ....-........-............................................................................ 2292 0 866 N..NT.C...CN.N....N....N..... 894 I D N G N V K N V S P Y Q S K E I M T Y Q G R C M L M L Q S Query_1 2494 ATAGACAATGGAAATGTAAAGAACGTATCTCCATATCAAAGCAAGGAGATTATGACATATCAGGGAAGATGTATGCTGATGCTTCAGTCA 2583 2 2293 .......................................................................................... 2382 T E K T S V L N I S A E T S E L Q S N K L T I N I K Query_1 2584 ACAGAAAAAACATCGGTACTGAATATCAGTGCAGAAACAAGTGAATTACAGTCGAATAAACTAACAATTAATATAAAA 2661 2 2383 .............................................................................. 2460 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 10970496 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 3,903 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200130.109 (CMR200130.32-851.P(851).E556Q.pMCSG68)
SDSNVDFNKDWKFVLKDSAHYSYTSYVPGDEWKKVNLPHDWSVGLPYDSISGEGCVAFLQGGIGWYSKSFPTTISANQKCYIVFDGVYNNSEYWINGKKLGYHLSGYAPFYFDVTDYLNPNEDNRMTVRVDHSHYADSRWYTGSGIYRDVKMIVTDRLHIPVWGTFVTTPVVTDKYAKVNNQITVRNSYSEPRTAVVEIVYKDNKGNIAAFEVFSIKLNAGEEKIIDIVSEIKQPDLWSVEIPVLYTAETRIKNGDEVISENTVRFGIRTFHFDADKGFFLNGKNMKIKGVCLHHDAGIVGTAMIRDVWYRRLKTLKEGGCNAIRLSHNPGADEFLSLCDEIGLLVQEEFFDEWDYPKDKRLNMKETVEDYPTHGYCEHFQEWAERDLKNVMRRSRNHACIFQWSIGNEIEWTYTGCREATGFFGADSNGNYFWNQPPYSKEKIREMWKIQPKQAYDIGRTAQKLAAWTRQMDTTRVVTANCILPSISFETGYIDALDVAGFSYRRVMYDYAKKNYPDKPIMGTENLGQWHEWKAVIERDFVPGMFIWTGVDYLGESGSRLSRWPQKSIGCGLLDMCGYVKPSYDMMKSLWTDKPFIAIYSQTPDKSSYLQVKDGFTDKKGHEWDRRLWVWDDVNSHWNYQKGDSVIVEIYSNCDEVELFVNGKSMGKKYIDDFEDHIYKWAVQYKPGTITAKGKNKLGNTTTAIRTSGKEHSILLAVDKQSIAANGKDVLHVTAQLTDKKGNPVKTTEQMLKFNIDGEYRLLGIDNGNVKNVSPYQSKEIMTYQGRCMLMLQSTEKTSVLNISAETSELQSNKLTINIK
TCTGATTCAAATGTTGATTTCAATAAAGATTGGAAATTCGTACTGAAAGATTCTGCTCATTATTCATATACTTCTTATGTCCCTGGTGATGAATGGAAGAAAGTGAACCTGCCACACGACTGGAGTGTTGGTCTGCCTTACGACTCCATCTCTGGCGAAGGGTGTGTAGCTTTCCTTCAGGGAGGAATAGGATGGTATAGCAAATCATTTCCCACAACAATCAGCGCAAATCAGAAATGCTATATAGTGTTCGATGGAGTATATAATAATTCTGAGTATTGGATAAATGGCAAAAAACTTGGATATCATCTTTCGGGATATGCTCCTTTTTATTTTGATGTCACAGACTATCTCAATCCCAATGAGGATAACCGCATGACTGTAAGGGTCGACCACAGCCATTATGCCGACAGCAGATGGTACACCGGTTCAGGTATATACAGGGATGTGAAAATGATTGTAACCGACAGACTGCATATTCCGGTTTGGGGAACATTTGTCACTACTCCCGTGGTTACTGATAAATATGCTAAAGTAAACAACCAAATTACCGTGCGCAACAGTTACTCTGAACCCAGAACAGCTGTTGTTGAGATAGTGTATAAAGATAATAAAGGCAATATCGCAGCCTTTGAGGTCTTCAGTATAAAACTGAATGCTGGTGAGGAGAAAATTATCGACATCGTATCGGAGATAAAACAGCCGGATTTGTGGAGCGTCGAGATACCAGTCCTCTATACAGCCGAGACCCGTATTAAGAATGGCGATGAAGTCATTTCTGAAAACACTGTCAGGTTCGGTATACGAACATTCCACTTTGATGCAGACAAAGGTTTCTTCCTTAACGGAAAAAATATGAAGATAAAAGGAGTATGCCTGCATCATGATGCCGGTATAGTTGGCACAGCAATGATACGCGATGTGTGGTACCGACGTCTGAAAACCCTTAAGGAAGGAGGATGTAACGCCATCCGCCTTTCGCACAATCCGGGAGCGGATGAGTTTCTGTCTTTGTGCGATGAGATAGGTCTTCTGGTCCAGGAAGAGTTCTTCGATGAGTGGGATTATCCCAAAGATAAAAGGCTCAATATGAAGGAAACGGTAGAAGACTATCCTACTCATGGTTATTGTGAGCATTTCCAGGAATGGGCTGAAAGGGATTTGAAAAACGTAATGAGGAGAAGCCGTAATCATGCCTGTATCTTCCAGTGGAGTATAGGTAATGAAATAGAATGGACTTATACCGGATGCCGTGAGGCAACAGGTTTCTTTGGAGCCGATTCCAACGGTAATTACTTCTGGAACCAGCCTCCATACTCTAAAGAAAAAATCAGAGAAATGTGGAAAATCCAGCCTAAACAAGCATACGACATTGGTCGTACAGCGCAAAAATTAGCAGCATGGACACGCCAGATGGATACTACACGAGTGGTTACCGCCAACTGCATCCTGCCTTCCATAAGTTTTGAGACAGGATATATCGATGCACTTGATGTGGCTGGTTTCAGCTACAGACGCGTGATGTATGATTATGCTAAGAAGAATTATCCTGACAAACCTATAATGGGTACAGAAAATCTTGGTCAGTGGCACGAATGGAAGGCGGTGATTGAAAGAGATTTCGTTCCGGGTATGTTTATATGGACAGGAGTCGATTATCTGGGAGAAAGTGGAAGCCGCCTTTCAAGATGGCCTCAAAAGTCAATAGGATGTGGTCTCCTGGATATGTGCGGCTATGTGAAGCCTTCGTACGACATGATGAAATCATTGTGGACTGACAAGCCTTTTATTGCTATATATTCACAGACTCCAGACAAATCTTCGTATCTCCAGGTAAAAGATGGCTTTACTGATAAGAAAGGACATGAATGGGATAGAAGATTATGGGTTTGGGATGATGTAAACTCTCACTGGAATTATCAGAAAGGTGACTCGGTAATAGTAGAAATATATTCCAATTGTGATGAAGTGGAACTTTTCGTTAACGGCAAGTCGATGGGAAAGAAGTATATAGACGATTTTGAGGATCATATCTATAAATGGGCAGTTCAGTACAAGCCTGGCACTATTACCGCAAAAGGAAAAAATAAGTTAGGTAATACCACTACAGCTATAAGGACTTCAGGCAAAGAACATTCGATATTGCTAGCGGTTGACAAACAAAGTATCGCAGCAAATGGAAAGGATGTTCTGCATGTCACAGCCCAGCTTACAGACAAAAAAGGTAATCCTGTAAAGACAACAGAACAGATGCTTAAGTTCAACATCGATGGAGAGTACCGTCTGTTGGGTATAGACAATGGAAATGTAAAGAACGTATCTCCATATCAAAGCAAGGAGATTATGACATATCAGGGAAGATGTATGCTGATGCTTCAGTCAACAGAAAAAACATCGGTACTGAATATCAGTGCAGAAACAAGTGAATTACAGTCGAATAAACTAACAATTAATATAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,040 total letters Query= CMR200130.109 Length=2865 Score E Sequences producing significant alignments: (Bits) Value wt 4428 0.0 11H_H8_CMR200130.109_H08.seq 24.7 0.51 08H_H8_CMR200130.109_H08.seq 24.7 0.51 S D S N V D F N K D W K F V L K D S A H Y S Y T S Y V P G D Query_1 202 TCTGATTCAAATGTTGATTTCAATAAAGATTGGAAATTCGTACTGAAAGATTCTGCTCATTATTCATATACTTCTTATGTCCCTGGTGAT 291 2 1 .......................................................................................... 90 2 2012 ........... 2002 E W K K V N L P H D W S V G L P Y D S I S G E G C V A F L Q Query_1 292 GAATGGAAGAAAGTGAACCTGCCACACGACTGGAGTGTTGGTCTGCCTTACGACTCCATCTCTGGCGAAGGGTGTGTAGCTTTCCTTCAG 381 2 91 .......................................................................................... 180 G G I G W Y S K S F P T T I S A N Q K C Y I V F D G V Y N N Query_1 382 GGAGGAATAGGATGGTATAGCAAATCATTTCCCACAACAATCAGCGCAAATCAGAAATGCTATATAGTGTTCGATGGAGTATATAATAAT 471 2 181 .......................................................................................... 270 S E Y W I N G K K L G Y H L S G Y A P F Y F D V T D Y L N P Query_1 472 TCTGAGTATTGGATAAATGGCAAAAAACTTGGATATCATCTTTCGGGATATGCTCCTTTTTATTTTGATGTCACAGACTATCTCAATCCC 561 2 271 .......................................................................................... 360 N E D N R M T V R V D H S H Y A D S R W Y T G S G I Y R D V Query_1 562 AATGAGGATAACCGCATGACTGTAAGGGTCGACCACAGCCATTATGCCGACAGCAGATGGTACACCGGTTCAGGTATATACAGGGATGTG 651 2 361 .......................................................................................... 450 K M I V T D R L H I P V W G T F V T T P V V T D K Y A K V N Query_1 652 AAAATGATTGTAACCGACAGACTGCATATTCCGGTTTGGGGAACATTTGTCACTACTCCCGTGGTTACTGATAAATATGCTAAAGTAAAC 741 2 451 .......................................................................................... 540 N Q I T V R N S Y S E P R T A V V E I V Y K D N K G N I A A Query_1 742 AACCAAATTACCGTGCGCAACAGTTACTCTGAACCCAGAACAGCTGTTGTTGAGATAGTGTATAAAGATAATAAAGGCAATATCGCAGCC 831 2 541 .......................................................................................... 630 F E V F S I K L N A G E E K I I D I V S E I K Q P D L W S V Query_1 832 TTTGAGGTCTTCAGTATAAAACTGAATGCTGGTGAGGAGAAAATTATCGACATCGTATCGGAGATAAAACAGCCGGATTTGTGGAGCGTC 921 2 631 .......................................................................................... 720 E I P V L Y T A E T R I K N G D E V I S E N T V R F G I R T Query_1 922 GAGATACCAGTCCTCTATACAGCCGAGACCCGTATTAAGAATGGCGATGAAGTCATTTCTGAAAACACTGTCAGGTTCGGTATACGAACA 1011 2 721 .......................................................................................... 810 F H F D A D K G F F L N G K N M K I K G V C L H H D A G I V Query_1 1012 TTCCACTTTGATGCAGACAAAGGTTTCTTCCTTAACGGAAAAAATATGAAGATAAAAGGAGTATGCCTGCATCATGATGCCGGTATAGTT 1101 2 811 .......................................................................................... 900 2 897 ...A..G............A..C... 872 2 1073 ........... 1083 G T A M I R D V W Y R R L K T L K E G G C N A I R L S H N P Query_1 1102 GGCACAGCAATGATACGCGATGTGTGGTACCGACGTCTGAAAACCCTTAAGGAAGGAGGATGTAACGCCATCCGCCTTTCGCACAATCCG 1191 2 901 .......................................................................................... 990 G A D E F L S L C D E I G L L V Q E E F F D E W D Y P K D K Query_1 1192 GGAGCGGATGAGTTTCTGTCTTTGTGCGATGAGATAGGTCTTCTGGTCCAGGAAGAGTTCTTCGATGAGTGGGATTATCCCAAAGATAAA 1281 2 991 .......................................................................................... 1080 R L N M K E T V E D Y P T H G Y C E H F Q E W A E R D L K N Query_1 1282 AGGCTCAATATGAAGGAAACGGTAGAAGACTATCCTACTCATGGTTATTGTGAGCATTTCCAGGAATGGGCTGAAAGGGATTTGAAAAAC 1371 2 1081 .......................................................................................... 1170 V M R R S R N H A C I F Q W S I G N E I E W T Y T G C R E A Query_1 1372 GTAATGAGGAGAAGCCGTAATCATGCCTGTATCTTCCAGTGGAGTATAGGTAATGAAATAGAATGGACTTATACCGGATGCCGTGAGGCA 1461 2 1171 .......................................................................................... 1260 1 639 ............A.. 625 T G F F G A D S N G N Y F W N Q P P Y S K E K I R E M W K I Query_1 1462 ACAGGTTTCTTTGGAGCCGATTCCAACGGTAATTACTTCTGGAACCAGCCTCCATACTCTAAAGAAAAAATCAGAGAAATGTGGAAAATC 1551 2 1261 .......................................................................................... 1350 Q P K Q A Y D I G R T A Q K L A A W T R Q M D T T R V V T A Query_1 1552 CAGCCTAAACAAGCATACGACATTGGTCGTACAGCGCAAAAATTAGCAGCATGGACACGCCAGATGGATACTACACGAGTGGTTACCGCC 1641 2 1351 .......................................................................................... 1440 N C I L P S I S F E T G Y I D A L D V A G F S Y R R V M Y D Query_1 1642 AACTGCATCCTGCCTTCCATAAGTTTTGAGACAGGATATATCGATGCACTTGATGTGGCTGGTTTCAGCTACAGACGCGTGATGTATGAT 1731 2 1441 .......................................................................................... 1530 Y A K K N Y P D K P I M G T Q N L G Q W H E W K A V I E R D Query_1 1732 TATGCTAAGAAGAATTATCCTGACAAACCTATAATGGGTACACAGAATCTTGGTCAGTGGCACGAATGGAAGGCGGTGATTGAAAGAGAT 1821 2 1531 ..........................................G.A............................................. 1620 F V P G M F I W T G V D Y L G E S G S R L S R W P Q K S I Query_1 1822 TTCGTTCCGGGTATGTTTATATGGACAGGAGTCGATTATCTGGGA-GAAAGTGGAAGCCGCCTTTCAAGATGGCCTCAAAAGTCAATAGG 1910 2 1621 .............................................-............................................ 1709 1 150 ..........G....... 133 0 28 ..........G....... 45 G C G L L D M C G Y V K P S Y D M M K S L W T D K P F I A I Query_1 1911 ATGTGGTCTCCTGGATATGTGCGGCTATGTGAAGCCTTCGTACGACATGATGAAATCATTGTGGACTGACAAGCCTTTTATTGCTATATA 2000 2 1710 .......................................................................................... 1799 Y S Q T P D K S S Y L Q V K D G F T D K K G H E W D R R L W Query_1 2001 TTCACAGACTCCAGACAAATCTTCGTATCTCCAGGTAAAAGATGGCTTTACTGATAAGAAAGGACATGAATGGGATAGAAGATTATGGGT 2090 2 1800 .......................................................................................... 1889 1 212 ............ 223 V W D D V N S H W N Y Q K G D S V I V E I Y S N C D E V E L Query_1 2091 TTGGGATGATGTAAACTCTCACTGGAATTATCAGAAAGGTGACTCGGTAATAGTAGAAATATATTCCAATTGTGATGAAGTGGAACTTTT 2180 2 1890 .......................................................................................... 1979 F V N G K S M G K K Y I D D F E D H I Y K W A V Q Y K P G T Query_1 2181 CGTTAACGGCAAGTCGATGGGAAAGAAGTATATAGACGATTTTGAGGATCATATCTATAAATGGGCAGTTCAGTACAAGCCTGGCACTAT 2270 2 1980 .......................................................................................... 2069 2 76 ........... 66 I T A K G K N K L G N T T T A I R T S G K E H S I L L A V D Query_1 2271 TACCGCAAAAGGAAAAAATAAGTTAGGTAATACCACTACAGCTATAAGGACTTCAGGCAAAGAACATTCGATATTGCTAGCGGTTGACAA 2360 2 2070 .......................................................................................... 2159 K Q S I A A N G K D V L H V T A Q L T D K K G N P V K T T E Query_1 2361 ACAAAGTATCGCAGCAAATGGAAAGGATGTTCTGCATGTCACAGCCCAGCTTACAGACAAAAAAGGTAATCCTGTAAAGACAACAGAACA 2450 2 2160 .......................................................................................... 2249 Q M L K F N I D G E Y R L L G I D N G N V K N V S P Y Q S K Query_1 2451 GATGCTTAAGTTCAACATCGATGGAGAGTACCGTCTGTTGGGTATAGACAATGGAAATGTAAAGAACGTATCTCCATATCAAAGCAAGGA 2540 2 2250 .......................................................................................... 2339 E I M T Y Q G R C M L M L Q S T E K T S V L N I S A E T S E Query_1 2541 GATTATGACATATCAGGGAAGATGTATGCTGATGCTTCAGTCAACAGAAAAAACATCGGTACTGAATATCAGTGCAGAAACAAGTGAATT 2630 2 2340 .......................................................................................... 2429 L Q S N K L T I N I K Query_1 2631 ACAGTCGAATAAACTAACAATTAATATAAAA 2661 2 2430 ............................... 2460 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 14208672 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 5,040 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200137.105 (CMR200137.19-931.P(931).E435A.pMCSG68)
QTDKIDLAGSWTFSTDSMDWSRVIELPGSMASNGFGEDIAVGTDWTGGIVDSSYFFKPSYAKYREAGNIKVPFWLQPVKYYKGKAWYQKEVVIPDSWEGKDISLFLERCHWESRLYIDGKEIGMQNALGAPHRYDLTGKLSAGKHVLMLCVDNRVKNIDPGENSHSISDHTQGNWNGVVGDMFLEVKPEVNVSSVKIMPERLAKKVSVSASLMNRYEKDANVVLEMTVGNEKVQQQCTLKPGENQVMMSLAMKGDIKCWDEFSPSLYDLKLSVKDADSGETDVYAERFGFRDVKVKDGKLTINDRRLFLRGTLDCAVFPKTGFPPTDVESWKKIYTTCRQHGLNHVRFHSWCPPEAAFAAADGMGMYLEIECSSWANQSTTIGDGGDLDRFIWEESERIVREFGNHPSFCMMMYGNEPAGEGSNAYLTNFVTTWKERDARRLYCSGAGWPNLPVNDFLSDSNPRIQAWGQGVKSIINAQAPRTDYDWSEYIGRFQQPMVSHEIGQWCVYPNFKEMAKYDGVMRPRNFEIFQETLAENGMAHLADSFLLASGKLQALCYKADIEAALRTKDFGGFQLLGLSDFPGQGTALVGVLDAFWEEKGYIRPEEYRRFCNSTVPLLRLPKLIYTNQETVKGSLEVAHFGAAPLEVTSTVWTLKTKEGKTIASGTLAHQPVGIGNCIPLGQLEIPLDKVDVPSCLTLEATLGDYANSWHIWVYPAAVQKVADEAQLLMTDRLDAKALQRLQEGGNVLLSLRKGSLPAEAGGEVVIGFSSIFWNTAWTLGQAPHTLGILCNPAHPALSEFPTEYYSDYQWWDAMSHSGAIEVVKIDKNLQPIVRVIDDWFTNRPLALLFEVKVGKGKLLVSGIDFWQDMDKRTEARQLLYSLKKYMCGNRFNPSSEVDAKDLSILFSIKNQK
CAGACTGATAAGATTGACCTGGCCGGCTCGTGGACATTTTCTACGGACAGCATGGACTGGAGCCGGGTGATTGAACTGCCGGGTTCAATGGCTTCCAATGGTTTTGGGGAAGATATTGCCGTGGGTACTGATTGGACGGGCGGTATTGTGGATTCTTCTTATTTCTTTAAACCTTCGTATGCCAAATACCGTGAGGCAGGAAATATCAAGGTACCTTTCTGGCTTCAGCCGGTAAAATATTACAAGGGTAAGGCGTGGTATCAGAAAGAGGTGGTGATTCCGGACAGTTGGGAAGGAAAGGACATTTCTCTCTTTTTGGAACGATGCCATTGGGAGAGCCGTTTGTATATAGACGGAAAGGAAATCGGCATGCAAAATGCTTTGGGGGCGCCCCATCGTTATGACCTGACAGGCAAGCTTTCAGCAGGGAAACATGTGTTGATGCTGTGTGTAGACAATCGGGTGAAAAACATTGATCCGGGGGAGAACTCACATAGTATTTCCGACCATACACAAGGAAACTGGAACGGGGTGGTAGGCGATATGTTCCTGGAAGTAAAGCCGGAAGTGAATGTGTCTTCCGTCAAGATTATGCCGGAGCGTCTGGCTAAGAAAGTCAGTGTGTCGGCTTCCTTGATGAACCGTTATGAAAAAGATGCCAATGTGGTACTGGAGATGACGGTAGGTAATGAAAAAGTACAGCAACAATGTACGTTGAAGCCGGGCGAAAATCAAGTGATGATGTCGCTGGCCATGAAGGGAGACATTAAGTGCTGGGATGAGTTTTCTCCATCCTTATATGATTTGAAGCTGAGTGTGAAGGATGCGGATAGCGGTGAAACGGATGTCTATGCGGAACGTTTTGGTTTCCGTGATGTGAAGGTGAAAGACGGCAAACTCACCATCAACGACCGCCGTTTGTTCCTGCGTGGTACGCTGGATTGTGCCGTATTTCCGAAGACCGGTTTCCCGCCCACGGATGTAGAATCCTGGAAAAAGATTTATACCACCTGTCGGCAGCACGGACTGAACCATGTGCGCTTCCATTCCTGGTGTCCGCCCGAAGCTGCTTTTGCAGCTGCCGATGGGATGGGTATGTACCTGGAGATAGAATGTTCTTCCTGGGCTAACCAGTCGACTACCATTGGCGATGGAGGCGATCTGGACCGCTTTATCTGGGAGGAAAGTGAACGCATCGTCCGTGAGTTTGGTAACCATCCTTCTTTCTGCATGATGATGTACGGTAACGAACCGGCTGGTGAGGGAAGTAATGCCTATCTGACTAATTTTGTTACTACCTGGAAAGAGCGCGATGCCCGCCGTTTATATTGTTCGGGTGCCGGATGGCCCAATTTGCCGGTTAACGACTTCTTGAGCGATTCCAATCCTCGTATTCAGGCGTGGGGACAAGGTGTGAAGAGTATTATCAACGCACAGGCTCCGCGTACCGACTATGACTGGTCAGAATACATCGGACGTTTCCAGCAGCCGATGGTGAGCCACGAAATCGGGCAGTGGTGTGTATATCCCAACTTCAAGGAAATGGCCAAATACGACGGGGTGATGCGCCCGCGTAATTTTGAGATATTCCAGGAAACACTGGCTGAAAACGGTATGGCACATTTGGCTGACAGCTTCCTGCTGGCTTCCGGAAAATTGCAGGCGTTGTGTTATAAGGCCGATATCGAAGCTGCTTTGCGTACAAAAGACTTCGGTGGATTCCAGTTACTGGGCTTGTCTGATTTCCCGGGGCAGGGTACGGCTTTGGTAGGAGTGCTCGATGCGTTCTGGGAAGAAAAAGGCTACATCCGTCCGGAAGAATACCGTCGTTTCTGTAATAGTACGGTACCATTACTGCGCTTGCCGAAGTTGATTTATACCAACCAGGAAACGGTGAAAGGAAGTCTGGAAGTGGCACATTTCGGAGCTGCTCCGCTGGAGGTGACTTCTACTGTCTGGACCCTGAAAACAAAAGAAGGAAAGACAATTGCTTCGGGCACGCTGGCACACCAGCCGGTAGGTATCGGCAATTGTATTCCGTTGGGGCAGCTGGAGATTCCATTGGATAAGGTGGACGTCCCTTCATGTCTGACACTGGAAGCTACATTGGGAGATTACGCCAACAGCTGGCACATCTGGGTATATCCTGCTGCGGTACAGAAAGTAGCTGATGAAGCACAATTGCTGATGACCGACCGTCTGGATGCAAAAGCTTTGCAACGTCTTCAGGAAGGTGGCAACGTACTGCTTTCTTTACGGAAAGGCTCCTTGCCTGCCGAAGCGGGAGGCGAAGTAGTGATAGGTTTCTCTAGCATCTTCTGGAACACGGCCTGGACGCTGGGACAAGCACCGCACACACTGGGTATCCTGTGTAACCCCGCTCATCCGGCACTTTCAGAGTTCCCTACAGAGTATTACAGTGATTATCAGTGGTGGGATGCCATGAGCCATTCCGGTGCCATCGAAGTGGTCAAGATTGATAAAAACTTGCAGCCGATTGTACGAGTTATCGACGACTGGTTTACGAACCGTCCGCTGGCTTTGTTGTTCGAAGTGAAGGTGGGTAAGGGTAAATTGCTTGTGTCAGGAATTGATTTCTGGCAGGATATGGACAAGCGTACGGAAGCCCGTCAGTTACTCTACAGCTTGAAGAAATATATGTGCGGTAATCGCTTCAATCCCTCTTCTGAAGTCGATGCGAAAGATTTAAGTATTTTGTTTTCCATTAAAAATCAAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,749 total letters Query= CMR200137.105 Length=3144 Score E Sequences producing significant alignments: (Bits) Value wt 4931 0.0 Q T D K I D L A G S W T F S T D S M D W S R V I E L P G S M Query_1 202 CAGACTGATAAGATTGACCTGGCCGGCTCGTGGACATTTTCTACGGACAGCATGGACTGGAGCCGGGTGATTGAACTGCCGGGTTCAATG 291 2 1 .......................................................................................... 90 A S N G F G E D I A V G T D W T G G I V D S S Y F F K P S Y Query_1 292 GCTTCCAATGGTTTTGGGGAAGATATTGCCGTGGGTACTGATTGGACGGGCGGTATTGTGGATTCTTCTTATTTCTTTAAACCTTCGTAT 381 2 91 .......................................................................................... 180 A K Y R E A G N I K V P F W L Q P V K Y Y K G K A W Y Q K E Query_1 382 GCCAAATACCGTGAGGCAGGAAATATCAAGGTACCTTTCTGGCTTCAGCCGGTAAAATATTACAAGGGTAAGGCGTGGTATCAGAAAGAG 471 2 181 .......................................................................................... 270 V V I P D S W E G K D I S L F L E R C H W E S R L Y I D G K Query_1 472 GTGGTGATTCCGGACAGTTGGGAAGGAAAGGACATTTCTCTCTTTTTGGAACGATGCCATTGGGAGAGCCGTTTGTATATAGACGGAAAG 561 2 271 .......................................................................................... 360 E I G M Q N A L G A P H R Y D L T G K L S A G K H V L M L C Query_1 562 GAAATCGGCATGCAAAATGCTTTGGGGGCGCCCCATCGTTATGACCTGACAGGCAAGCTTTCAGCAGGGAAACATGTGTTGATGCTGTGT 651 2 361 .......................................................................................... 450 V D N R V K N I D P G E N S H S I S D H T Q G N W N G V V G Query_1 652 GTAGACAATCGGGTGAAAAACATTGATCCGGGGGAGAACTCACATAGTATTTCCGACCATACACAAGGAAACTGGAACGGGGTGGTAGGC 741 2 451 .......................................................................................... 540 D M F L E V K P E V N V S S V K I M P E R L A K K V S V S A Query_1 742 GATATGTTCCTGGAAGTAAAGCCGGAAGTGAATGTGTCTTCCGTCAAGATTATGCCGGAGCGTCTGGCTAAGAAAGTCAGTGTGTCGGCT 831 2 541 .......................................................................................... 630 S L M N R Y E K D A N V V L E M T V G N E K V Q Q Q C T L K Query_1 832 TCCTTGATGAACCGTTATGAAAAAGATGCCAATGTGGTACTGGAGATGACGGTAGGTAATGAAAAAGTACAGCAACAATGTACGTTGAAG 921 2 631 .......................................................................................... 720 P G E N Q V M M S L A M K G D I K C W D E F S P S L Y D L K Query_1 922 CCGGGCGAAAATCAAGTGATGATGTCGCTGGCCATGAAGGGAGACATTAAGTGCTGGGATGAGTTTTCTCCATCCTTATATGATTTGAAG 1011 2 721 .......................................................................................... 810 L S V K D A D S G E T D V Y A E R F G F R D V K V K D G K L Query_1 1012 CTGAGTGTGAAGGATGCGGATAGCGGTGAAACGGATGTCTATGCGGAACGTTTTGGTTTCCGTGATGTGAAGGTGAAAGACGGCAAACTC 1101 2 811 .......................................................................................... 900 T I N D R R L F L R G T L D C A V F P K T G F P P T D V E S Query_1 1102 ACCATCAACGACCGCCGTTTGTTCCTGCGTGGTACGCTGGATTGTGCCGTATTTCCGAAGACCGGTTTCCCGCCCACGGATGTAGAATCC 1191 2 901 .......................................................................................... 990 W K K I Y T T C R Q H G L N H V R F H S W C P P E A A F A A Query_1 1192 TGGAAAAAGATTTATACCACCTGTCGGCAGCACGGACTGAACCATGTGCGCTTCCATTCCTGGTGTCCGCCCGAAGCTGCTTTTGCAGCT 1281 2 991 .......................................................................................... 1080 2 1686 ............ 1697 2 1872 ........... 1882 A D G M G M Y L E I E C S S W A N Q S T T I G D G G D L D R Query_1 1282 GCCGATGGGATGGGTATGTACCTGGAGATAGAATGTTCTTCCTGGGCTAACCAGTCGACTACCATTGGCGATGGAGGCGATCTGGACCGC 1371 2 1081 .......................................................................................... 1170 F I W E E S E R I V R E F G N H P S F C M M M Y G N A P A G Query_1 1372 TTTATCTGGGAGGAAAGTGAACGCATCGTCCGTGAGTTTGGTAACCATCCTTCTTTCTGCATGATGATGTACGGTAACGCTCCGGCTGGT 1461 2 1171 ...............................................................................AA......... 1260 E G S N A Y L T N F V T T W K E R D A R R L Y C S G A G W P Query_1 1462 GAGGGAAGTAATGCCTATCTGACTAATTTTGTTACTACCTGGAAAGAGCGCGATGCCCGCCGTTTATATTGTTCGGGTGCCGGATGGCCC 1551 2 1261 .......................................................................................... 1350 N L P V N D F L S D S N P R I Q A W G Q G V K S I I N A Q A Query_1 1552 AATTTGCCGGTTAACGACTTCTTGAGCGATTCCAATCCTCGTATTCAGGCGTGGGGACAAGGTGTGAAGAGTATTATCAACGCACAGGCT 1641 2 1351 .......................................................................................... 1440 P R T D Y D W S E Y I G R F Q Q P M V S H E I G Q W C V Y P Query_1 1642 CCGCGTACCGACTATGACTGGTCAGAATACATCGGACGTTTCCAGCAGCCGATGGTGAGCCACGAAATCGGGCAGTGGTGTGTATATCCC 1731 2 1441 .......................................................................................... 1530 N F K E M A K Y D G V M R P R N F E I F Q E T L A E N G M A Query_1 1732 AACTTCAAGGAAATGGCCAAATACGACGGGGTGATGCGCCCGCGTAATTTTGAGATATTCCAGGAAACACTGGCTGAAAACGGTATGGCA 1821 2 1531 .......................................................................................... 1620 H L A D S F L L A S G K L Q A L C Y K A D I E A A L R T K D Query_1 1822 CATTTGGCTGACAGCTTCCTGCTGGCTTCCGGAAAATTGCAGGCGTTGTGTTATAAGGCCGATATCGAAGCTGCTTTGCGTACAAAAGAC 1911 2 1621 .......................................................................................... 1710 2 1062 ............ 1073 F G G F Q L L G L S D F P G Q G T A L V G V L D A F W E E K Query_1 1912 TTCGGTGGATTCCAGTTACTGGGCTTGTCTGATTTCCCGGGGCAGGGTACGGCTTTGGTAGGAGTGCTCGATGCGTTCTGGGAAGAAAAA 2001 2 1711 .......................................................................................... 1800 G Y I R P E E Y R R F C N S T V P L L R L P K L I Y T N Q E Query_1 2002 GGCTACATCCGTCCGGAAGAATACCGTCGTTTCTGTAATAGTACGGTACCATTACTGCGCTTGCCGAAGTTGATTTATACCAACCAGGAA 2091 2 1801 .......................................................................................... 1890 2 999 ........... 1009 T V K G S L E V A H F G A A P L E V T S T V W T L K T K E G Query_1 2092 ACGGTGAAAGGAAGTCTGGAAGTGGCACATTTCGGAGCTGCTCCGCTGGAGGTGACTTCTACTGTCTGGACCCTGAAAACAAAAGAAGGA 2181 2 1891 .......................................................................................... 1980 K T I A S G T L A H Q P V G I G N C I P L G Q L E I P L D K Query_1 2182 AAGACAATTGCTTCGGGCACGCTGGCACACCAGCCGGTAGGTATCGGCAATTGTATTCCGTTGGGGCAGCTGGAGATTCCATTGGATAAG 2271 2 1981 .......................................................................................... 2070 2 1261 ........... 1251 V D V P S C L T L E A T L G D Y A N S W H I W V Y P A A V Q Query_1 2272 GTGGACGTCCCTTCATGTCTGACACTGGAAGCTACATTGGGAGATTACGCCAACAGCTGGCACATCTGGGTATATCCTGCTGCGGTACAG 2361 2 2071 .......................................................................................... 2160 K V A D E A Q L L M T D R L D A K A L Q R L Q E G G N V L L Query_1 2362 AAAGTAGCTGATGAAGCACAATTGCTGATGACCGACCGTCTGGATGCAAAAGCTTTGCAACGTCTTCAGGAAGGTGGCAACGTACTGCTT 2451 2 2161 .......................................................................................... 2250 S L R K G S L P A E A G G E V V I G F S S I F W N T A W T L Query_1 2452 TCTTTACGGAAAGGCTCCTTGCCTGCCGAAGCGGGAGGCGAAGTAGTGATAGGTTTCTCTAGCATCTTCTGGAACACGGCCTGGACGCTG 2541 2 2251 .......................................................................................... 2340 G Q A P H T L G I L C N P A H P A L S E F P T E Y Y S D Y Q Query_1 2542 GGACAAGCACCGCACACACTGGGTATCCTGTGTAACCCCGCTCATCCGGCACTTTCAGAGTTCCCTACAGAGTATTACAGTGATTATCAG 2631 2 2341 .......................................................................................... 2430 W W D A M S H S G A I E V V K I D K N L Q P I V R V I D D W Query_1 2632 TGGTGGGATGCCATGAGCCATTCCGGTGCCATCGAAGTGGTCAAGATTGATAAAAACTTGCAGCCGATTGTACGAGTTATCGACGACTGG 2721 2 2431 .......................................................................................... 2520 F T N R P L A L L F E V K V G K G K L L V S G I D F W Q D M Query_1 2722 TTTACGAACCGTCCGCTGGCTTTGTTGTTCGAAGTGAAGGTGGGTAAGGGTAAATTGCTTGTGTCAGGAATTGATTTCTGGCAGGATATG 2811 2 2521 .......................................................................................... 2610 D K R T E A R Q L L Y S L K K Y M C G N R F N P S S E V D A Query_1 2812 GACAAGCGTACGGAAGCCCGTCAGTTACTCTACAGCTTGAAGAAATATATGTGCGGTAATCGCTTCAATCCCTCTTCTGAAGTCGATGCG 2901 2 2611 .......................................................................................... 2700 K D L S I L F S I K N Q K Query_1 2902 AAAGATTTAAGTATTTTGTTTTCCATTAAAAATCAAAAA 2940 2 2701 ....................................... 2739 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 8436646 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 2,749 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200137.106 (CMR200137.19-931.P(931).E435Q.pMCSG68)
QTDKIDLAGSWTFSTDSMDWSRVIELPGSMASNGFGEDIAVGTDWTGGIVDSSYFFKPSYAKYREAGNIKVPFWLQPVKYYKGKAWYQKEVVIPDSWEGKDISLFLERCHWESRLYIDGKEIGMQNALGAPHRYDLTGKLSAGKHVLMLCVDNRVKNIDPGENSHSISDHTQGNWNGVVGDMFLEVKPEVNVSSVKIMPERLAKKVSVSASLMNRYEKDANVVLEMTVGNEKVQQQCTLKPGENQVMMSLAMKGDIKCWDEFSPSLYDLKLSVKDADSGETDVYAERFGFRDVKVKDGKLTINDRRLFLRGTLDCAVFPKTGFPPTDVESWKKIYTTCRQHGLNHVRFHSWCPPEAAFAAADGMGMYLEIECSSWANQSTTIGDGGDLDRFIWEESERIVREFGNHPSFCMMMYGNEPAGEGSNAYLTNFVTTWKERDARRLYCSGAGWPNLPVNDFLSDSNPRIQAWGQGVKSIINAQAPRTDYDWSEYIGRFQQPMVSHEIGQWCVYPNFKEMAKYDGVMRPRNFEIFQETLAENGMAHLADSFLLASGKLQALCYKADIEAALRTKDFGGFQLLGLSDFPGQGTALVGVLDAFWEEKGYIRPEEYRRFCNSTVPLLRLPKLIYTNQETVKGSLEVAHFGAAPLEVTSTVWTLKTKEGKTIASGTLAHQPVGIGNCIPLGQLEIPLDKVDVPSCLTLEATLGDYANSWHIWVYPAAVQKVADEAQLLMTDRLDAKALQRLQEGGNVLLSLRKGSLPAEAGGEVVIGFSSIFWNTAWTLGQAPHTLGILCNPAHPALSEFPTEYYSDYQWWDAMSHSGAIEVVKIDKNLQPIVRVIDDWFTNRPLALLFEVKVGKGKLLVSGIDFWQDMDKRTEARQLLYSLKKYMCGNRFNPSSEVDAKDLSILFSIKNQK
CAGACTGATAAGATTGACCTGGCCGGCTCGTGGACATTTTCTACGGACAGCATGGACTGGAGCCGGGTGATTGAACTGCCGGGTTCAATGGCTTCCAATGGTTTTGGGGAAGATATTGCCGTGGGTACTGATTGGACGGGCGGTATTGTGGATTCTTCTTATTTCTTTAAACCTTCGTATGCCAAATACCGTGAGGCAGGAAATATCAAGGTACCTTTCTGGCTTCAGCCGGTAAAATATTACAAGGGTAAGGCGTGGTATCAGAAAGAGGTGGTGATTCCGGACAGTTGGGAAGGAAAGGACATTTCTCTCTTTTTGGAACGATGCCATTGGGAGAGCCGTTTGTATATAGACGGAAAGGAAATCGGCATGCAAAATGCTTTGGGGGCGCCCCATCGTTATGACCTGACAGGCAAGCTTTCAGCAGGGAAACATGTGTTGATGCTGTGTGTAGACAATCGGGTGAAAAACATTGATCCGGGGGAGAACTCACATAGTATTTCCGACCATACACAAGGAAACTGGAACGGGGTGGTAGGCGATATGTTCCTGGAAGTAAAGCCGGAAGTGAATGTGTCTTCCGTCAAGATTATGCCGGAGCGTCTGGCTAAGAAAGTCAGTGTGTCGGCTTCCTTGATGAACCGTTATGAAAAAGATGCCAATGTGGTACTGGAGATGACGGTAGGTAATGAAAAAGTACAGCAACAATGTACGTTGAAGCCGGGCGAAAATCAAGTGATGATGTCGCTGGCCATGAAGGGAGACATTAAGTGCTGGGATGAGTTTTCTCCATCCTTATATGATTTGAAGCTGAGTGTGAAGGATGCGGATAGCGGTGAAACGGATGTCTATGCGGAACGTTTTGGTTTCCGTGATGTGAAGGTGAAAGACGGCAAACTCACCATCAACGACCGCCGTTTGTTCCTGCGTGGTACGCTGGATTGTGCCGTATTTCCGAAGACCGGTTTCCCGCCCACGGATGTAGAATCCTGGAAAAAGATTTATACCACCTGTCGGCAGCACGGACTGAACCATGTGCGCTTCCATTCCTGGTGTCCGCCCGAAGCTGCTTTTGCAGCTGCCGATGGGATGGGTATGTACCTGGAGATAGAATGTTCTTCCTGGGCTAACCAGTCGACTACCATTGGCGATGGAGGCGATCTGGACCGCTTTATCTGGGAGGAAAGTGAACGCATCGTCCGTGAGTTTGGTAACCATCCTTCTTTCTGCATGATGATGTACGGTAACGAACCGGCTGGTGAGGGAAGTAATGCCTATCTGACTAATTTTGTTACTACCTGGAAAGAGCGCGATGCCCGCCGTTTATATTGTTCGGGTGCCGGATGGCCCAATTTGCCGGTTAACGACTTCTTGAGCGATTCCAATCCTCGTATTCAGGCGTGGGGACAAGGTGTGAAGAGTATTATCAACGCACAGGCTCCGCGTACCGACTATGACTGGTCAGAATACATCGGACGTTTCCAGCAGCCGATGGTGAGCCACGAAATCGGGCAGTGGTGTGTATATCCCAACTTCAAGGAAATGGCCAAATACGACGGGGTGATGCGCCCGCGTAATTTTGAGATATTCCAGGAAACACTGGCTGAAAACGGTATGGCACATTTGGCTGACAGCTTCCTGCTGGCTTCCGGAAAATTGCAGGCGTTGTGTTATAAGGCCGATATCGAAGCTGCTTTGCGTACAAAAGACTTCGGTGGATTCCAGTTACTGGGCTTGTCTGATTTCCCGGGGCAGGGTACGGCTTTGGTAGGAGTGCTCGATGCGTTCTGGGAAGAAAAAGGCTACATCCGTCCGGAAGAATACCGTCGTTTCTGTAATAGTACGGTACCATTACTGCGCTTGCCGAAGTTGATTTATACCAACCAGGAAACGGTGAAAGGAAGTCTGGAAGTGGCACATTTCGGAGCTGCTCCGCTGGAGGTGACTTCTACTGTCTGGACCCTGAAAACAAAAGAAGGAAAGACAATTGCTTCGGGCACGCTGGCACACCAGCCGGTAGGTATCGGCAATTGTATTCCGTTGGGGCAGCTGGAGATTCCATTGGATAAGGTGGACGTCCCTTCATGTCTGACACTGGAAGCTACATTGGGAGATTACGCCAACAGCTGGCACATCTGGGTATATCCTGCTGCGGTACAGAAAGTAGCTGATGAAGCACAATTGCTGATGACCGACCGTCTGGATGCAAAAGCTTTGCAACGTCTTCAGGAAGGTGGCAACGTACTGCTTTCTTTACGGAAAGGCTCCTTGCCTGCCGAAGCGGGAGGCGAAGTAGTGATAGGTTTCTCTAGCATCTTCTGGAACACGGCCTGGACGCTGGGACAAGCACCGCACACACTGGGTATCCTGTGTAACCCCGCTCATCCGGCACTTTCAGAGTTCCCTACAGAGTATTACAGTGATTATCAGTGGTGGGATGCCATGAGCCATTCCGGTGCCATCGAAGTGGTCAAGATTGATAAAAACTTGCAGCCGATTGTACGAGTTATCGACGACTGGTTTACGAACCGTCCGCTGGCTTTGTTGTTCGAAGTGAAGGTGGGTAAGGGTAAATTGCTTGTGTCAGGAATTGATTTCTGGCAGGATATGGACAAGCGTACGGAAGCCCGTCAGTTACTCTACAGCTTGAAGAAATATATGTGCGGTAATCGCTTCAATCCCTCTTCTGAAGTCGATGCGAAAGATTTAAGTATTTTGTTTTCCATTAAAAATCAAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,174 total letters Query= CMR200137.106 Length=3144 Score E Sequences producing significant alignments: (Bits) Value wt 4931 0.0 12B_B9_CMR200137.106_B09.seq 1874 0.0 09B_B9_CMR200137.106_B09.seq 1788 0.0 Q T D K I D L A G S W T F S T D S M D W S R V I E L P G S M Query_1 202 CAGACTGATAAGATTGACCTGGCCGGCTCGTGGACATTTTCTACGGACAGCATGGACTGGAGCCGGGTGATTGAACTGCCGGGTTCAATG 291 2 1 .......................................................................................... 90 A S N G F G E D I A V G T D W T G G I V D S S Y F F K P S Y Query_1 292 GCTTCCAATGGTTTTGGGGAAGATATTGCCGTGGGTACTGATTGGACGGGCGGTATTGTGGATTCTTCTTATTTCTTTAAACCTTCGTAT 381 2 91 .......................................................................................... 180 A K Y R E A G N I K V P F W L Q P V K Y Y K G K A W Y Q K E Query_1 382 GCCAAATACCGTGAGGCAGGAAATATCAAGGTACCTTTCTGGCTTCAGCCGGTAAAATATTACAAGGGTAAGGCGTGGTATCAGAAAGAG 471 2 181 .......................................................................................... 270 V V I P D S W E G K D I S L F L E R C H W E S R L Y I D G K Query_1 472 GTGGTGATTCCGGACAGTTGGGAAGGAAAGGACATTTCTCTCTTTTTGGAACGATGCCATTGGGAGAGCCGTTTGTATATAGACGGAAAG 561 2 271 .......................................................................................... 360 E I G M Q N A L G A P H R Y D L T G K L S A G K H V L M L C Query_1 562 GAAATCGGCATGCAAAATGCTTTGGGGGCGCCCCATCGTTATGACCTGACAGGCAAGCTTTCAGCAGGGAAACATGTGTTGATGCTGTGT 651 2 361 .......................................................................................... 450 V D N R V K N I D P G E N S H S I S D H T Q G N W N G V V G Query_1 652 GTAGACAATCGGGTGAAAAACATTGATCCGGGGGAGAACTCACATAGTATTTCCGACCATACACAAGGAAACTGGAACGGGGTGGTAGGC 741 2 451 .......................................................................................... 540 1 1165 ....N....N.-. 1154 D M F L E V K P E V N V S S V K I M P E R L A K K V S V S Query_1 742 GATATGTTCCTGGAAGTAAAGCCGGAAGTG-AATGTGTCTTCCGTCAAGATTATGCCGGAGCGTCTGGCTAAGAAAGTCAGTGTGTCGGC 830 2 541 ..............................-........................................................... 629 1 1153 ....N...NNN...N.N..-.N.NN.....G...NNN...-..........................CT...............N..... 1066 A S L M N R Y E K D A N V V L E M T V G N E K V Q Q Q C T L Query_1 831 -TTCCTTGATGAACCGTTATGAAAAAGATGCCAATGTGGTACTGGAGATGACGGTAGGTAATGAAAAAGTACAGCAACAATGTACGTTGA 919 2 630 -......................................................................................... 718 1 1065 C...T.........G....N--...........N......................N................................. 978 K P G E N Q V M M S L A M K G D I K C W D E F S P S L Y D Query_1 920 AGCCGGGCGAAAATCAAGTGATGATGTCGCT-GGCCATGAAGGGAGACATTAAGTGCTGGGATGAGTTTTCTCC-ATCCTTATATGATTT 1007 2 719 ...............................-..........................................-............... 806 1 977 ...............................N.........................N................C............... 888 L K L S V K D A D S G E T D V Y A E R F G F R D V K V K D G Query_1 1008 GAAGCTGAGTGTGAAGGATGCGGATAGCGGTGAAACGGATGTCTATGCGGAACGTTTTGGTTTCCGTGATGTGAAGGTGAAAGACGGCAA 1097 2 807 .......................................................................................... 896 1 887 ................N............-.............................N.............................. 799 K L T I N D R R L F L R G T L D C A V F P K T G F P P T D V Query_1 1098 ACTCACCATCAACGACCGCCGTTTGTTCCTGCGTGGTACGCTGGATTGTGCCGTATTTCCGAAGACCGGTTTCCCGCCCACGGATGTAGA 1187 2 897 .......................................................................................... 986 1 798 .......................................................................................... 709 E S W K K I Y T T C R Q H G L N H V R F H S W C P P E A A F Query_1 1188 ATCCTGGAAAAAGATTTATACCACCTGTCGGCAGCACGGACTGAACCATGTGCGCTTCCATTCCTGGTGTCCGCCCGAAGCTGCTTTTGC 1277 2 987 .......................................................................................... 1076 2 1686 ............ 1697 2 1872 ........... 1882 1 708 .......................................................................................... 619 0 544 ............ 555 0 731 ........... 741 A A A D G M G M Y L E I E C S S W A N Q S T T I G D G G D L Query_1 1278 AGCTGCCGATGGGATGGGTATGTACCTGGAGATAGAATGTTCTTCCTGGGCTAACCAGTCGACTACCATTGGCGATGGAGGCGATCTGGA 1367 2 1077 .......................................................................................... 1166 1 618 .......................................................................................... 529 0 14 ....N..N...G 25 D R F I W E E S E R I V R E F G N H P S F C M M M Y G N Q P Query_1 1368 CCGCTTTATCTGGGAGGAAAGTGAACGCATCGTCCGTGAGTTTGGTAACCATCCTTCTTTCTGCATGATGATGTACGGTAACCAGCCGGC 1457 2 1167 ..................................................................................G.A..... 1256 1 528 ..................................................................................G.A..... 439 0 26 N......N-.........................................................................G.A..... 114 A G E G S N A Y L T N F V T T W K E R D A R R L Y C S G A G Query_1 1458 TGGTGAGGGAAGTAATGCCTATCTGACTAATTTTGTTACTACCTGGAAAGAGCGCGATGCCCGCCGTTTATATTGTTCGGGTGCCGGATG 1547 2 1257 .......................................................................................... 1346 1 438 .......................................................................................... 349 0 115 .......................................................................................... 204 W P N L P V N D F L S D S N P R I Q A W G Q G V K S I I N A Query_1 1548 GCCCAATTTGCCGGTTAACGACTTCTTGAGCGATTCCAATCCTCGTATTCAGGCGTGGGGACAAGGTGTGAAGAGTATTATCAACGCACA 1637 2 1347 .......................................................................................... 1436 1 348 .......................................................................................... 259 0 205 .......................................................................................... 294 Q A P R T D Y D W S E Y I G R F Q Q P M V S H E I G Q W C V Query_1 1638 GGCTCCGCGTACCGACTATGACTGGTCAGAATACATCGGACGTTTCCAGCAGCCGATGGTGAGCCACGAAATCGGGCAGTGGTGTGTATA 1727 2 1437 .......................................................................................... 1526 1 258 .......................................................................................... 169 0 295 .......................................................................................... 384 Y P N F K E M A K Y D G V M R P R N F E I F Q E T L A E N G Query_1 1728 TCCCAACTTCAAGGAAATGGCCAAATACGACGGGGTGATGCGCCCGCGTAATTTTGAGATATTCCAGGAAACACTGGCTGAAAACGGTAT 1817 2 1527 .......................................................................................... 1616 1 168 ...................................................................C.G.................... 79 0 385 ...................................................................C.N.................... 474 M A H L A D S F L L A S G K L Q A L C Y K A D I E A A L R T Query_1 1818 GGCACATTTGGCTGACAGCTTCCTGCTGGCTTCCGGAAAATTGCAGGCGTTGTGTTATAAGGCCGATATCGAAGCTGCTTTGCGTACAAA 1907 2 1617 .......................................................................................... 1706 2 1062 ............ 1073 1 78 ........................................-............N..-N...... 17 1 633 ............ 622 0 475 .......................................................................................... 564 K D F G G F Q L L G L S D F P G Q G T A L V G V L D A F W E Query_1 1908 AGACTTCGGTGGATTCCAGTTACTGGGCTTGTCTGATTTCCCGGGGCAGGGTACGGCTTTGGTAGGAGTGCTCGATGCGTTCTGGGAAGA 1997 2 1707 .......................................................................................... 1796 0 565 .......................................................................................... 654 E K G Y I R P E E Y R R F C N S T V P L L R L P K L I Y T N Query_1 1998 AAAAGGCTACATCCGTCCGGAAGAATACCGTCGTTTCTGTAATAGTACGGTACCA-TTACTGCGCTTGCCGAAGTTGATTTATACCAACC 2086 2 1797 .......................................................-.................................. 1885 2 999 ........... 1009 1 696 ........... 686 0 655 ............................................N..........T.................................. 744 Q E T V K G S L E V A H F G A A P L E V T S T V W T L K T K Query_1 2087 AGGAAACGGTGAAAGGAAGTCTGGAAGTGGCACATTTCGGAGCTGCTCCGCTGGAGGTGACTTCTACTGTCTGGACCCTGAAAACAAAAG 2176 2 1886 .......................................................................................... 1975 0 745 .....................................................................................-.... 833 E G K T I A S G T L A H Q P V G I G N C I P L G Q L E I P L Query_1 2177 AAGGAAAGACAATTGCTTCGGGCACGCTGGCACACCAGCCGGTAGGTATCGGCAATTGTATTCCGTTGGGGCAGCTGGAGATTCCATTGG 2266 2 1976 .......................................................................................... 2065 1 434 ........... 444 0 834 ...................-.....................................................N..............-. 921 0 119 ........... 109 D K V D V P S C L T L E A T L G D Y A N S W H I W V Y P A A Query_1 2267 ATAAGGTGGACGTCCCTTCATGTCTGACACTGGAAGCTACATTGGGAGATTACGCCAACAGCTGGCACATCTGGGTATATCCTGCTGCGG 2356 2 2066 .......................................................................................... 2155 0 922 ....-........T...............N.-......N..................C..........N.........N..T......-. 1008 V Q K V A D E A Q L L M T D R L D A K A L Q R L Q E G G N V Query_1 2357 TACAGAAAGTAGCTGATGAAGCACAATTGCTGATGACCGACCGTCTGG-ATGCAAAAGCTTTGCAACGTCTTCAGGAAGGTGGCAACGTA 2445 2 2156 ................................................-......................................... 2244 0 1009 .....-................NN.T......................A.....T........-N........N.--.N..T..N..... 1094 L L S L R K G S L P A E A G G E V V I G F S S I F W N T A W Query_1 2446 CTGCTTTCTTTACGGAAAGGCTCCTTGCCTGCCGAAGCGGGAGGCGAAGTAGTGATAGGTTTCTCTAGCATCTTCTGGAACACGGCCTGG 2535 2 2245 .......................................................................................... 2334 0 1095 ........-.....--....N...A..-.N...... 1126 T L G Q A P H T L G I L C N P A H P A L S E F P T E Y Y S D Query_1 2536 ACGCTGGGACAAGCACCGCACACACTGGGTATCCTGTGTAACCCCGCTCATCCGGCACTTTCAGAGTTCCCTACAGAGTATTACAGTGAT 2625 2 2335 .......................................................................................... 2424 Y Q W W D A M S H S G A I E V V K I D K N L Q P I V R V I D Query_1 2626 TATCAGTGGTGGGATGCCATGAGCCATTCCGGTGCCATCGAAGTGGTCAAGATTGATAAAAACTTGCAGCCGATTGTACGAGTTATCGAC 2715 2 2425 .......................................................................................... 2514 D W F T N R P L A L L F E V K V G K G K L L V S G I D F W Q Query_1 2716 GACTGGTTTACGAACCGTCCGCTGGCTTTGTTGTTCGAAGTGAAGGTGGGTAAGGGTAAATTGCTTGTGTCAGGAATTGATTTCTGGCAG 2805 2 2515 .......................................................................................... 2604 D M D K R T E A R Q L L Y S L K K Y M C G N R F N P S S E V Query_1 2806 GATATGGACAAGCGTACGGAAGCCCGTCAGTTACTCTACAGCTTGAAGAAATATATGTGCGGTAATCGCTTCAATCCCTCTTCTGAAGTC 2895 2 2605 .......................................................................................... 2694 D A K D L S I L F S I K N Q K Query_1 2896 GATGCGAAAGATTTAAGTATTTTGTTTTCCATTAAAAATCAAAAA 2940 2 2695 ............................................. 2739 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 16005120 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 5,174 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200137.108 (CMR200137.19-931.P(931).E550Q.pMCSG68)
QTDKIDLAGSWTFSTDSMDWSRVIELPGSMASNGFGEDIAVGTDWTGGIVDSSYFFKPSYAKYREAGNIKVPFWLQPVKYYKGKAWYQKEVVIPDSWEGKDISLFLERCHWESRLYIDGKEIGMQNALGAPHRYDLTGKLSAGKHVLMLCVDNRVKNIDPGENSHSISDHTQGNWNGVVGDMFLEVKPEVNVSSVKIMPERLAKKVSVSASLMNRYEKDANVVLEMTVGNEKVQQQCTLKPGENQVMMSLAMKGDIKCWDEFSPSLYDLKLSVKDADSGETDVYAERFGFRDVKVKDGKLTINDRRLFLRGTLDCAVFPKTGFPPTDVESWKKIYTTCRQHGLNHVRFHSWCPPEAAFAAADGMGMYLEIECSSWANQSTTIGDGGDLDRFIWEESERIVREFGNHPSFCMMMYGNEPAGEGSNAYLTNFVTTWKERDARRLYCSGAGWPNLPVNDFLSDSNPRIQAWGQGVKSIINAQAPRTDYDWSEYIGRFQQPMVSHEIGQWCVYPNFKEMAKYDGVMRPRNFEIFQETLAENGMAHLADSFLLASGKLQALCYKADIEAALRTKDFGGFQLLGLSDFPGQGTALVGVLDAFWEEKGYIRPEEYRRFCNSTVPLLRLPKLIYTNQETVKGSLEVAHFGAAPLEVTSTVWTLKTKEGKTIASGTLAHQPVGIGNCIPLGQLEIPLDKVDVPSCLTLEATLGDYANSWHIWVYPAAVQKVADEAQLLMTDRLDAKALQRLQEGGNVLLSLRKGSLPAEAGGEVVIGFSSIFWNTAWTLGQAPHTLGILCNPAHPALSEFPTEYYSDYQWWDAMSHSGAIEVVKIDKNLQPIVRVIDDWFTNRPLALLFEVKVGKGKLLVSGIDFWQDMDKRTEARQLLYSLKKYMCGNRFNPSSEVDAKDLSILFSIKNQK
CAGACTGATAAGATTGACCTGGCCGGCTCGTGGACATTTTCTACGGACAGCATGGACTGGAGCCGGGTGATTGAACTGCCGGGTTCAATGGCTTCCAATGGTTTTGGGGAAGATATTGCCGTGGGTACTGATTGGACGGGCGGTATTGTGGATTCTTCTTATTTCTTTAAACCTTCGTATGCCAAATACCGTGAGGCAGGAAATATCAAGGTACCTTTCTGGCTTCAGCCGGTAAAATATTACAAGGGTAAGGCGTGGTATCAGAAAGAGGTGGTGATTCCGGACAGTTGGGAAGGAAAGGACATTTCTCTCTTTTTGGAACGATGCCATTGGGAGAGCCGTTTGTATATAGACGGAAAGGAAATCGGCATGCAAAATGCTTTGGGGGCGCCCCATCGTTATGACCTGACAGGCAAGCTTTCAGCAGGGAAACATGTGTTGATGCTGTGTGTAGACAATCGGGTGAAAAACATTGATCCGGGGGAGAACTCACATAGTATTTCCGACCATACACAAGGAAACTGGAACGGGGTGGTAGGCGATATGTTCCTGGAAGTAAAGCCGGAAGTGAATGTGTCTTCCGTCAAGATTATGCCGGAGCGTCTGGCTAAGAAAGTCAGTGTGTCGGCTTCCTTGATGAACCGTTATGAAAAAGATGCCAATGTGGTACTGGAGATGACGGTAGGTAATGAAAAAGTACAGCAACAATGTACGTTGAAGCCGGGCGAAAATCAAGTGATGATGTCGCTGGCCATGAAGGGAGACATTAAGTGCTGGGATGAGTTTTCTCCATCCTTATATGATTTGAAGCTGAGTGTGAAGGATGCGGATAGCGGTGAAACGGATGTCTATGCGGAACGTTTTGGTTTCCGTGATGTGAAGGTGAAAGACGGCAAACTCACCATCAACGACCGCCGTTTGTTCCTGCGTGGTACGCTGGATTGTGCCGTATTTCCGAAGACCGGTTTCCCGCCCACGGATGTAGAATCCTGGAAAAAGATTTATACCACCTGTCGGCAGCACGGACTGAACCATGTGCGCTTCCATTCCTGGTGTCCGCCCGAAGCTGCTTTTGCAGCTGCCGATGGGATGGGTATGTACCTGGAGATAGAATGTTCTTCCTGGGCTAACCAGTCGACTACCATTGGCGATGGAGGCGATCTGGACCGCTTTATCTGGGAGGAAAGTGAACGCATCGTCCGTGAGTTTGGTAACCATCCTTCTTTCTGCATGATGATGTACGGTAACGAACCGGCTGGTGAGGGAAGTAATGCCTATCTGACTAATTTTGTTACTACCTGGAAAGAGCGCGATGCCCGCCGTTTATATTGTTCGGGTGCCGGATGGCCCAATTTGCCGGTTAACGACTTCTTGAGCGATTCCAATCCTCGTATTCAGGCGTGGGGACAAGGTGTGAAGAGTATTATCAACGCACAGGCTCCGCGTACCGACTATGACTGGTCAGAATACATCGGACGTTTCCAGCAGCCGATGGTGAGCCACGAAATCGGGCAGTGGTGTGTATATCCCAACTTCAAGGAAATGGCCAAATACGACGGGGTGATGCGCCCGCGTAATTTTGAGATATTCCAGGAAACACTGGCTGAAAACGGTATGGCACATTTGGCTGACAGCTTCCTGCTGGCTTCCGGAAAATTGCAGGCGTTGTGTTATAAGGCCGATATCGAAGCTGCTTTGCGTACAAAAGACTTCGGTGGATTCCAGTTACTGGGCTTGTCTGATTTCCCGGGGCAGGGTACGGCTTTGGTAGGAGTGCTCGATGCGTTCTGGGAAGAAAAAGGCTACATCCGTCCGGAAGAATACCGTCGTTTCTGTAATAGTACGGTACCATTACTGCGCTTGCCGAAGTTGATTTATACCAACCAGGAAACGGTGAAAGGAAGTCTGGAAGTGGCACATTTCGGAGCTGCTCCGCTGGAGGTGACTTCTACTGTCTGGACCCTGAAAACAAAAGAAGGAAAGACAATTGCTTCGGGCACGCTGGCACACCAGCCGGTAGGTATCGGCAATTGTATTCCGTTGGGGCAGCTGGAGATTCCATTGGATAAGGTGGACGTCCCTTCATGTCTGACACTGGAAGCTACATTGGGAGATTACGCCAACAGCTGGCACATCTGGGTATATCCTGCTGCGGTACAGAAAGTAGCTGATGAAGCACAATTGCTGATGACCGACCGTCTGGATGCAAAAGCTTTGCAACGTCTTCAGGAAGGTGGCAACGTACTGCTTTCTTTACGGAAAGGCTCCTTGCCTGCCGAAGCGGGAGGCGAAGTAGTGATAGGTTTCTCTAGCATCTTCTGGAACACGGCCTGGACGCTGGGACAAGCACCGCACACACTGGGTATCCTGTGTAACCCCGCTCATCCGGCACTTTCAGAGTTCCCTACAGAGTATTACAGTGATTATCAGTGGTGGGATGCCATGAGCCATTCCGGTGCCATCGAAGTGGTCAAGATTGATAAAAACTTGCAGCCGATTGTACGAGTTATCGACGACTGGTTTACGAACCGTCCGCTGGCTTTGTTGTTCGAAGTGAAGGTGGGTAAGGGTAAATTGCTTGTGTCAGGAATTGATTTCTGGCAGGATATGGACAAGCGTACGGAAGCCCGTCAGTTACTCTACAGCTTGAAGAAATATATGTGCGGTAATCGCTTCAATCCCTCTTCTGAAGTCGATGCGAAAGATTTAAGTATTTTGTTTTCCATTAAAAATCAAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,246 total letters Query= CMR200137.108 Length=3144 Score E Sequences producing significant alignments: (Bits) Value wt 4931 0.0 12C_C9_CMR200137.108_C09.seq 1849 0.0 09C_C9_CMR200137.108_C09.seq 1817 0.0 Q T D K I D L A G S W T F S T D S M D W S R V I E L P G S M Query_1 202 CAGACTGATAAGATTGACCTGGCCGGCTCGTGGACATTTTCTACGGACAGCATGGACTGGAGCCGGGTGATTGAACTGCCGGGTTCAATG 291 2 1 .......................................................................................... 90 A S N G F G E D I A V G T D W T G G I V D S S Y F F K P S Y Query_1 292 GCTTCCAATGGTTTTGGGGAAGATATTGCCGTGGGTACTGATTGGACGGGCGGTATTGTGGATTCTTCTTATTTCTTTAAACCTTCGTAT 381 2 91 .......................................................................................... 180 A K Y R E A G N I K V P F W L Q P V K Y Y K G K A W Y Q K E Query_1 382 GCCAAATACCGTGAGGCAGGAAATATCAAGGTACCTTTCTGGCTTCAGCCGGTAAAATATTACAAGGGTAAGGCGTGGTATCAGAAAGAG 471 2 181 .......................................................................................... 270 V V I P D S W E G K D I S L F L E R C H W E S R L Y I D G K Query_1 472 GTGGTGATTCCGGACAGTTGGGAAGGAAAGGACATTTCTCTCTTTTTGGAACGATGCCATTGGGAGAGCCGTTTGTATATAGACGGAAAG 561 2 271 .......................................................................................... 360 E I G M Q N A L G A P H R Y D L T G K L S A G K H V L M L C Query_1 562 GAAATCGGCATGCAAAATGCTTTGGGGGCGCCCCATCGTTATGACCTGACAGGCAAGCTTTCAGCAGGGAAACATGTGTTGATGCTGTGT 651 2 361 .......................................................................................... 450 V D N R V K N I D P G E N S H S I S D H T Q G N W N G V V Query_1 652 GTAGACAATCGGGTGAAAAACATTGATCCGGGGGAGAACTCACATAGTATTTCCGACCATACACAAGGAAACTGGAACGGGGTGGT-AGG 740 2 451 ......................................................................................-... 539 1 1167 .......N... 1157 G D M F L E V K P E V N V S S V K I M P E R L A K K V S V Query_1 741 CGATAT--GTTCCTGGAAGTAAAGCCGGAAGTGAATGTGTCTTCCGTCAAGATTATGCC-GGAGCGTCTGGCTAAGAAAGTCAGTGTG-T 826 2 540 ......--...................................................-............................-. 625 1 1156 ......AGN........-....G....A.NN..G.A-..NN.NN.....N.........C....N...................GN..G. 1069 S A S L M N R Y E K D A N V V L E M T V G N E K V Q Q Q C Query_1 827 CGGCTTCCTTGATGAACCGTTATGAAAAAG-ATG-CCAATGTGGTACTGGAGATGACGG-TAGGTAATGAAAAAGTACAGCAACAATGTA 913 2 626 ..............................-...-........................-.............................. 712 1 1068 ..N...............NG..........G...G........-.....NN........N.............-................ 981 T L K P G E N Q V M M S L A M K G D I K C W D E F S P S L Y Query_1 914 CGTTGAAGCCGGGCGAAAATCAAGTGATGATGTCGCTGGCC-ATGAAGGGAGACATTAAGTGCTGGGATGAGTTTTCTCCATCCTTATAT 1002 2 713 .........................................-................................................ 801 1 980 ...-..........................N..........C................................................ 892 D L K L S V K D A D S G E T D V Y A E R F G F R D V K V K D Query_1 1003 GATTTGAAGCTGAGTGTGAAGGATGCGGATAGCGGTGAAACGGATGTCTATGCGGAACGTTTTGGTTTCCGTGATGTGAAGGTGAAAGAC 1092 2 802 .......................................................................................... 891 1 891 ..............................................................N.-......................... 803 G K L T I N D R R L F L R G T L D C A V F P K T G F P P T Query_1 1093 GGCAAACTCACCATCAACGACCGCCGTTT-GTTCCTGCGTGGTACGCTGGATTGTGCCGTATTTCCGAAGACCGGTTTCCCGCCCACGGA 1181 2 892 .............................-............................................................ 980 1 802 .............................T............................................................ 713 D V E S W K K I Y T T C R Q H G L N H V R F H S W C P P E A Query_1 1182 TGTAGAATCCTGGAAAAAGATTTATACCACCTGTCGGCAGCACGGACTGAACCATGTGCGCTTCCATTCCTGGTGTCCGCCCGAAGCTGC 1271 2 981 .......................................................................................... 1070 2 1686 ......... 1694 2 1872 ........... 1882 1 712 .......................................................................................... 623 0 544 ......... 552 0 730 ........... 740 A F A A A D G M G M Y L E I E C S S W A N Q S T T I G D G G Query_1 1272 TTTTGCAGCTGCCGATGGGATGGGTATGTACCTGGAGATAGAATGTTCTTCCTGGGCTAACCAGTCGACTACCATTGGCGATGGAGGCGA 1361 2 1071 .......................................................................................... 1160 2 1695 ... 1697 1 622 .......................................................................................... 533 0 15 .... 18 0 553 ... 555 D L D R F I W E E S E R I V R E F G N H P S F C M M M Y G N Query_1 1362 TCTGGACCGCTTTATCTGGGAGGAAAGTGAACGCATCGTCCGTGAGTTTGGTAACCATCCTTCTTTCTGCATGATGATGTACGGTAACGA 1451 2 1161 .......................................................................................... 1250 1 532 .......................................................................................... 443 0 19 .......................................................................................... 108 E P A G E G S N A Y L T N F V T T W K E R D A R R L Y C S G Query_1 1452 ACCGGCTGGTGAGGGAAGTAATGCCTATCTGACTAATTTTGTTACTACCTGGAAAGAGCGCGATGCCCGCCGTTTATATTGTTCGGGTGC 1541 2 1251 .......................................................................................... 1340 1 442 .......................................................................................... 353 0 109 .......................................................................................... 198 0 877 ........... 867 A G W P N L P V N D F L S D S N P R I Q A W G Q G V K S I I Query_1 1542 CGGATGGCCCAATTTGCCGGTTAACGACTTCTTGAGCGATTCCAATCCTCGTATTCAGGCGTGGGGACAAGGTGTGAAGAGTATTATCAA 1631 2 1341 .......................................................................................... 1430 1 352 .......................................................................................... 263 0 199 .......................................................................................... 288 N A Q A P R T D Y D W S E Y I G R F Q Q P M V S H E I G Q W Query_1 1632 CGCACAGGCTCCGCGTACCGACTATGACTGGTCAGAATACATCGGACGTTTCCAGCAGCCGATGGTGAGCCACGAAATCGGGCAGTGGTG 1721 2 1431 .......................................................................................... 1520 1 262 .......................................................................................... 173 0 289 .......................................................................................... 378 C V Y P N F K E M A K Y D G V M R P R N F E I F Q Q T L A E Query_1 1722 TGTATATCCCAACTTCAAGGAAATGGCCAAATACGACGGGGTGATGCGCCCGCGTAATTTTGAGATATTCCAGCAGACACTGGCTGAAAA 1811 2 1521 .........................................................................G.A.............. 1610 1 172 .........................................................................GCT.............. 83 0 379 .........................................................................GCT.............. 468 N G M A H L A D S F L L A S G K L Q A L C Y K A D I E A A L Query_1 1812 CGGTATGGCACATTTGGCTGACAGCTTCCTGCTGGCTTCCGGAAAATTGCAGGCGTTGTGTTATAAGGCCGATATCGAAGCTGCTTTGCG 1901 2 1611 .......................................................................................... 1700 2 1062 ............ 1073 1 82 .......................................................... 25 1 631 ............ 620 0 469 .......................................................................................... 558 R T K D F G G F Q L L G L S D F P G Q G T A L V G V L D A F Query_1 1902 TACAAAAGACTTCGGTGGATTCCAGTTACTGGGCTTGTCTGATTTCCCGGGGCAGGGTACGGCTTTGGTAGGAGTGCTCGATGCGTTCTG 1991 2 1701 .......................................................................................... 1790 0 559 .......................................................................................... 648 W E E K G Y I R P E E Y R R F C N S T V P L L R L P K L I Y Query_1 1992 GGAAGAAAAAGGCTACATCCGTCCGGAAGAATACCGTCGTTTCTGTAATAGTACGGTACCATTACTGCGCTTGCCGAAGTTGATTTATAC 2081 2 1791 .......................................................................................... 1880 2 999 ......... 1007 1 694 ......... 686 0 649 .......................................................................................... 738 T N Q E T V K G S L E V A H F G A A P L E V T S T V W T L K Query_1 2082 CAACCAGGAAACGGTGAAAGG-AAGTCTGGAAGTGGCACATTTCGGAGCTGCTCCGCTGGAGGTGACTTCTACTGTCTGGACCCTGAAAA 2170 2 1881 .....................-.................................................................... 1969 2 1008 .. 1009 1 685 .. 684 0 739 .....................A.......................................N...........................N 828 T K E G K T I A S G T L A H Q P V G I G N C I P L G Q L E I Query_1 2171 CAAAAGAAGGAAAGACAATTGCTTCGGGCACGCTGGCACACCAGCCGGTAGGTATCGGCAATTGTATT-CCGTTGGGGCAGCTGGAGATT 2259 2 1970 ....................................................................-..................... 2058 0 829 ........N.........................................A.................C..................... 918 0 119 ........... 109 P L D K V D V P S C L T L E A T L G D Y A N S W H I W V Y Query_1 2260 CCATTGGATAAGGTGGACGTCCCTTCATGTCTGACACTGGAAG-CTACATT-GGGAGATTACGCCAACAGCTGGCACATCTGGGTATATC 2347 2 2059 ...........................................-.......-...................................... 2146 0 919 ............TG.A..N......NN............T...N.......N..............N..N...................- 1007 P A A V Q K V A D E A Q L L M T D R L D A K A L Q R L Q E Query_1 2348 CTGCTGCGGTACAGAAAGTAGCTGATGAAGCACAATTGCTGATGACCGACCGTCTGGATGCAAAAGCTTTGCAACGTCTTCAGGAA--GG 2435 2 2147 ......................................................................................--.. 2234 0 1008 .......N.........N...........N....T.................N.....AN.....N.......NNNNN...N....AN.. 1097 G G N V L L S L R K G S L P A E A G G E V V I G F S S I F Query_1 2436 TGGCAACGTACTG-CTTTCTTTACGGAAAGG--CTCCTTGCCTG-CCGAAGCGGGAGGCGAAGTAGTGATAGGTTTCTCTAGCATCTTCT 2521 2 2235 .............-.................--...........-............................................. 2320 0 1098 N.C........N.NN..............ANCT.....N.N...N..... 1147 W N T A W T L G Q A P H T L G I L C N P A H P A L S E F P T Query_1 2522 GGAACACGGCCTGGACGCTGGGACAAGCACCGCACACACTGGGTATCCTGTGTAACCCCGCTCATCCGGCACTTTCAGAGTTCCCTACAG 2611 2 2321 .......................................................................................... 2410 E Y Y S D Y Q W W D A M S H S G A I E V V K I D K N L Q P I Query_1 2612 AGTATTACAGTGATTATCAGTGGTGGGATGCCATGAGCCATTCCGGTGCCATCGAAGTGGTCAAGATTGATAAAAACTTGCAGCCGATTG 2701 2 2411 .......................................................................................... 2500 V R V I D D W F T N R P L A L L F E V K V G K G K L L V S G Query_1 2702 TACGAGTTATCGACGACTGGTTTACGAACCGTCCGCTGGCTTTGTTGTTCGAAGTGAAGGTGGGTAAGGGTAAATTGCTTGTGTCAGGAA 2791 2 2501 .......................................................................................... 2590 I D F W Q D M D K R T E A R Q L L Y S L K K Y M C G N R F N Query_1 2792 TTGATTTCTGGCAGGATATGGACAAGCGTACGGAAGCCCGTCAGTTACTCTACAGCTTGAAGAAATATATGTGCGGTAATCGCTTCAATC 2881 2 2591 .......................................................................................... 2680 P S S E V D A K D L S I L F S I K N Q K Query_1 2882 CCTCTTCTGAAGTCGATGCGAAAGATTTAAGTATTTTGTTTTCCATTAAAAATCAAAAA 2940 2 2681 ........................................................... 2739 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 16230192 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 5,246 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200137.112 (CMR200137.19-931.P(931).E550A.pMCSG68)
QTDKIDLAGSWTFSTDSMDWSRVIELPGSMASNGFGEDIAVGTDWTGGIVDSSYFFKPSYAKYREAGNIKVPFWLQPVKYYKGKAWYQKEVVIPDSWEGKDISLFLERCHWESRLYIDGKEIGMQNALGAPHRYDLTGKLSAGKHVLMLCVDNRVKNIDPGENSHSISDHTQGNWNGVVGDMFLEVKPEVNVSSVKIMPERLAKKVSVSASLMNRYEKDANVVLEMTVGNEKVQQQCTLKPGENQVMMSLAMKGDIKCWDEFSPSLYDLKLSVKDADSGETDVYAERFGFRDVKVKDGKLTINDRRLFLRGTLDCAVFPKTGFPPTDVESWKKIYTTCRQHGLNHVRFHSWCPPEAAFAAADGMGMYLEIECSSWANQSTTIGDGGDLDRFIWEESERIVREFGNHPSFCMMMYGNEPAGEGSNAYLTNFVTTWKERDARRLYCSGAGWPNLPVNDFLSDSNPRIQAWGQGVKSIINAQAPRTDYDWSEYIGRFQQPMVSHEIGQWCVYPNFKEMAKYDGVMRPRNFEIFQETLAENGMAHLADSFLLASGKLQALCYKADIEAALRTKDFGGFQLLGLSDFPGQGTALVGVLDAFWEEKGYIRPEEYRRFCNSTVPLLRLPKLIYTNQETVKGSLEVAHFGAAPLEVTSTVWTLKTKEGKTIASGTLAHQPVGIGNCIPLGQLEIPLDKVDVPSCLTLEATLGDYANSWHIWVYPAAVQKVADEAQLLMTDRLDAKALQRLQEGGNVLLSLRKGSLPAEAGGEVVIGFSSIFWNTAWTLGQAPHTLGILCNPAHPALSEFPTEYYSDYQWWDAMSHSGAIEVVKIDKNLQPIVRVIDDWFTNRPLALLFEVKVGKGKLLVSGIDFWQDMDKRTEARQLLYSLKKYMCGNRFNPSSEVDAKDLSILFSIKNQK
CAGACTGATAAGATTGACCTGGCCGGCTCGTGGACATTTTCTACGGACAGCATGGACTGGAGCCGGGTGATTGAACTGCCGGGTTCAATGGCTTCCAATGGTTTTGGGGAAGATATTGCCGTGGGTACTGATTGGACGGGCGGTATTGTGGATTCTTCTTATTTCTTTAAACCTTCGTATGCCAAATACCGTGAGGCAGGAAATATCAAGGTACCTTTCTGGCTTCAGCCGGTAAAATATTACAAGGGTAAGGCGTGGTATCAGAAAGAGGTGGTGATTCCGGACAGTTGGGAAGGAAAGGACATTTCTCTCTTTTTGGAACGATGCCATTGGGAGAGCCGTTTGTATATAGACGGAAAGGAAATCGGCATGCAAAATGCTTTGGGGGCGCCCCATCGTTATGACCTGACAGGCAAGCTTTCAGCAGGGAAACATGTGTTGATGCTGTGTGTAGACAATCGGGTGAAAAACATTGATCCGGGGGAGAACTCACATAGTATTTCCGACCATACACAAGGAAACTGGAACGGGGTGGTAGGCGATATGTTCCTGGAAGTAAAGCCGGAAGTGAATGTGTCTTCCGTCAAGATTATGCCGGAGCGTCTGGCTAAGAAAGTCAGTGTGTCGGCTTCCTTGATGAACCGTTATGAAAAAGATGCCAATGTGGTACTGGAGATGACGGTAGGTAATGAAAAAGTACAGCAACAATGTACGTTGAAGCCGGGCGAAAATCAAGTGATGATGTCGCTGGCCATGAAGGGAGACATTAAGTGCTGGGATGAGTTTTCTCCATCCTTATATGATTTGAAGCTGAGTGTGAAGGATGCGGATAGCGGTGAAACGGATGTCTATGCGGAACGTTTTGGTTTCCGTGATGTGAAGGTGAAAGACGGCAAACTCACCATCAACGACCGCCGTTTGTTCCTGCGTGGTACGCTGGATTGTGCCGTATTTCCGAAGACCGGTTTCCCGCCCACGGATGTAGAATCCTGGAAAAAGATTTATACCACCTGTCGGCAGCACGGACTGAACCATGTGCGCTTCCATTCCTGGTGTCCGCCCGAAGCTGCTTTTGCAGCTGCCGATGGGATGGGTATGTACCTGGAGATAGAATGTTCTTCCTGGGCTAACCAGTCGACTACCATTGGCGATGGAGGCGATCTGGACCGCTTTATCTGGGAGGAAAGTGAACGCATCGTCCGTGAGTTTGGTAACCATCCTTCTTTCTGCATGATGATGTACGGTAACGAACCGGCTGGTGAGGGAAGTAATGCCTATCTGACTAATTTTGTTACTACCTGGAAAGAGCGCGATGCCCGCCGTTTATATTGTTCGGGTGCCGGATGGCCCAATTTGCCGGTTAACGACTTCTTGAGCGATTCCAATCCTCGTATTCAGGCGTGGGGACAAGGTGTGAAGAGTATTATCAACGCACAGGCTCCGCGTACCGACTATGACTGGTCAGAATACATCGGACGTTTCCAGCAGCCGATGGTGAGCCACGAAATCGGGCAGTGGTGTGTATATCCCAACTTCAAGGAAATGGCCAAATACGACGGGGTGATGCGCCCGCGTAATTTTGAGATATTCCAGGAAACACTGGCTGAAAACGGTATGGCACATTTGGCTGACAGCTTCCTGCTGGCTTCCGGAAAATTGCAGGCGTTGTGTTATAAGGCCGATATCGAAGCTGCTTTGCGTACAAAAGACTTCGGTGGATTCCAGTTACTGGGCTTGTCTGATTTCCCGGGGCAGGGTACGGCTTTGGTAGGAGTGCTCGATGCGTTCTGGGAAGAAAAAGGCTACATCCGTCCGGAAGAATACCGTCGTTTCTGTAATAGTACGGTACCATTACTGCGCTTGCCGAAGTTGATTTATACCAACCAGGAAACGGTGAAAGGAAGTCTGGAAGTGGCACATTTCGGAGCTGCTCCGCTGGAGGTGACTTCTACTGTCTGGACCCTGAAAACAAAAGAAGGAAAGACAATTGCTTCGGGCACGCTGGCACACCAGCCGGTAGGTATCGGCAATTGTATTCCGTTGGGGCAGCTGGAGATTCCATTGGATAAGGTGGACGTCCCTTCATGTCTGACACTGGAAGCTACATTGGGAGATTACGCCAACAGCTGGCACATCTGGGTATATCCTGCTGCGGTACAGAAAGTAGCTGATGAAGCACAATTGCTGATGACCGACCGTCTGGATGCAAAAGCTTTGCAACGTCTTCAGGAAGGTGGCAACGTACTGCTTTCTTTACGGAAAGGCTCCTTGCCTGCCGAAGCGGGAGGCGAAGTAGTGATAGGTTTCTCTAGCATCTTCTGGAACACGGCCTGGACGCTGGGACAAGCACCGCACACACTGGGTATCCTGTGTAACCCCGCTCATCCGGCACTTTCAGAGTTCCCTACAGAGTATTACAGTGATTATCAGTGGTGGGATGCCATGAGCCATTCCGGTGCCATCGAAGTGGTCAAGATTGATAAAAACTTGCAGCCGATTGTACGAGTTATCGACGACTGGTTTACGAACCGTCCGCTGGCTTTGTTGTTCGAAGTGAAGGTGGGTAAGGGTAAATTGCTTGTGTCAGGAATTGATTTCTGGCAGGATATGGACAAGCGTACGGAAGCCCGTCAGTTACTCTACAGCTTGAAGAAATATATGTGCGGTAATCGCTTCAATCCCTCTTCTGAAGTCGATGCGAAAGATTTAAGTATTTTGTTTTCCATTAAAAATCAAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,368 total letters Query= CMR200137.112 Length=3144 Score E Sequences producing significant alignments: (Bits) Value wt 4931 0.0 12D_D9_CMR200137.112_D09.seq 1824 0.0 09D_D9_CMR200137.112_D09.seq 1440 0.0 Q T D K I D L A G S W T F S T D S M D W S R V I E L P G S M Query_1 202 CAGACTGATAAGATTGACCTGGCCGGCTCGTGGACATTTTCTACGGACAGCATGGACTGGAGCCGGGTGATTGAACTGCCGGGTTCAATG 291 2 1 .......................................................................................... 90 A S N G F G E D I A V G T D W T G G I V D S S Y F F K P S Y Query_1 292 GCTTCCAATGGTTTTGGGGAAGATATTGCCGTGGGTACTGATTGGACGGGCGGTATTGTGGATTCTTCTTATTTCTTTAAACCTTCGTAT 381 2 91 .......................................................................................... 180 A K Y R E A G N I K V P F W L Q P V K Y Y K G K A W Y Q K E Query_1 382 GCCAAATACCGTGAGGCAGGAAATATCAAGGTACCTTTCTGGCTTCAGCCGGTAAAATATTACAAGGGTAAGGCGTGGTATCAGAAAGAG 471 2 181 .......................................................................................... 270 V V I P D S W E G K D I S L F L E R C H W E S R L Y I D G K Query_1 472 GTGGTGATTCCGGACAGTTGGGAAGGAAAGGACATTTCTCTCTTTTTGGAACGATGCCATTGGGAGAGCCGTTTGTATATAGACGGAAAG 561 2 271 .......................................................................................... 360 E I G M Q N A L G A P H R Y D L T G K L S A G K H V L M L C Query_1 562 GAAATCGGCATGCAAAATGCTTTGGGGGCGCCCCATCGTTATGACCTGACAGGCAAGCTTTCAGCAGGGAAACATGTGTTGATGCTGTGT 651 2 361 .......................................................................................... 450 V D N R V K N I D P G E N S H S I S D H T Q G N W N G V V G Query_1 652 GTAGACAATCGGGTGAAAAACATTGATCCGGGGGAGAACTCACATAGTATTTCCGACCATACACAAGGAAACTGGAACGGGGTGGTAGGC 741 2 451 .......................................................................................... 540 D M F L E V K P E V N V S S V K I M P E R L A K K V S V S Query_1 742 GATATGTTCCTGGAAGTAAAGCCGGAAGTGAA-TG-TGTCTTCCGTCAAGATTATGCCGGAGCGTCTGGCTAAGAAAGTCAGTGTGTCGG 829 2 541 ................................-..-...................................................... 628 1 1149 .........A....N......AN...A..N.T.........N..N...NN...............T.....NN.N...NT.... 1066 A S L M N R Y E K D A N V V L E M T V G N E K V Q Q Q C T L Query_1 830 CTTCCTTGATGAACCGTTATGAAAAAGATGCCAATGTGGTACTGGAGATGACGGTAGGTAATGAAAAAGTACAGCAACAATGTACGTTGA 919 2 629 .......................................................................................... 718 1 1065 .......N.................-.N.N...N......N.N.............................N...N..-.......... 978 K P G E N Q V M M S L A M K G D I K C W D E F S P S L Y D Query_1 920 AGCCGGGCGAAAATCAAGTGATGATGTCGCTGGCCATGAAGGG-AGACATTAA-GTGCTGGGATGAGTTTTCTCCATCCTTATATGATTT 1007 2 719 ...........................................-.........-.................................... 806 1 977 ..G.C......................................G.....N...A.................................... 888 L K L S V K D A D S G E T D V Y A E R F G F R D V K V K D G Query_1 1008 GAA-GCTGAGTGTGAAGGATGCGGATAGCGGTGAAACGGATGTCTATGCGGAACGTTTTGGTTTCCGTGATGTGAAGGTGAAAGACGGCA 1096 2 807 ...-...................................................................................... 895 1 887 ...A......N.............................................................NA................ 798 K L T I N D R R L F L R G T L D C A V F P K T G F P P T D V Query_1 1097 AA-CTCACCATCAACGACCGCCGTTTGTTCCTGCGTGGTACGCTGGATTGTGCCGTATTTCCGAAGACCGGTTTCCCGCCCACGGATGTA 1185 2 896 ..-....................................................................................... 984 1 797 ..A....................................................................................... 708 E S W K K I Y T T C R Q H G L N H V R F H S W C P P E A A F Query_1 1186 GAATCCTGGAAAAAGATTTATACCACCTGTCGGCAGCACGGACTGAACCATGTGCGCTTCCATTCCTGGTGTCCGCCCGAAGCTGCTTTT 1275 2 985 .......................................................................................... 1074 2 1686 ............ 1697 2 1872 ........... 1882 1 707 .......................................................................................... 618 0 540 ............ 551 0 728 ........... 738 A A A D G M G M Y L E I E C S S W A N Q S T T I G D G G D L Query_1 1276 GCAGCTGCCGATGGGATGGGTATGTACCTGGAGATAGAATGTTCTTCCTGGGCTAACCAGTCGACTACCATTGGCGATGGAGGCGATCTG 1365 2 1075 .......................................................................................... 1164 1 617 .......................................................................................... 528 0 11 ..... 15 D R F I W E E S E R I V R E F G N H P S F C M M M Y G N E Query_1 1366 GACCGCTTTATCTGGGAGGAAAGTGAACGCATCG-TCCGTGAGTTTGG-TAACCATCCTTCTTTCTGCATGATGATGTACGGT-AACGAA 1452 2 1165 ..................................-.............-..................................-...... 1251 1 527 ..................................-.............-..................................-...... 441 0 16 ...........................N......C.............C.....N............................T...... 105 P A G E G S N A Y L T N F V T T W K E R D A R R L Y C S G A Query_1 1453 CCGGCTGGTGAGGGAAGTAATGCCTATCTGACTAATTTTGTTACTACCTGGAAAGAGCGCGATGCCCGCCGTTTATATTGTTCGGGTGCC 1542 2 1252 .......................................................................................... 1341 1 440 .......................................................................................... 351 0 106 .......................................................................................... 195 G W P N L P V N D F L S D S N P R I Q A W G Q G V K S I I N Query_1 1543 GGATGGCCCAATTTGCCGGTTAACGACTTCTTGAGCGATTCCAATCCTCGTATTCAGGCGTGGGGACAAGGTGTGAAGAGTATTATCAAC 1632 2 1342 .......................................................................................... 1431 1 350 .......................................................................................... 261 0 196 .......................................................................................... 285 A Q A P R T D Y D W S E Y I G R F Q Q P M V S H E I G Q W C Query_1 1633 GCACAGGCTCCGCGTACCGACTATGACTGGTCAGAATACATCGGACGTTTCCAGCAGCCGATGGTGAGCCACGAAATCGGGCAGTGGTGT 1722 2 1432 .......................................................................................... 1521 1 260 ......................T................................................................... 171 0 286 ......................T................................................................... 375 V Y P N F K E M A K Y D G V M R P R N F E I F Q A T L A E N Query_1 1723 GTATATCCCAACTTCAAGGAAATGGCCAAATACGACGGGGTGATGCGCCCGCGTAATTTTGAGATATTCCAGGCTACACTGGCTGAAAAC 1812 2 1522 .........................................................................AA............... 1611 1 170 .........................................................................AA............... 81 0 376 .................................N.......................................AA............... 465 G M A H L A D S F L L A S G K L Q A L C Y K A D I E A A L R Query_1 1813 GGTATGGCACATTTGGCTGACAGCTTCCTGCTGGCTTCCGGAAAATTGCAGGCGTTGTGTTATAAGGCCGATATCGAAGCTGCTTTGCGT 1902 2 1612 .......................................................................................... 1701 2 1062 ............ 1073 1 80 .............................................. 35 0 466 .......................................................................................... 555 T K D F G G F Q L L G L S D F P G Q G T A L V G V L D A F W Query_1 1903 ACAAAAGACTTCGGTGGATTCCAGTTACTGGGCTTGTCTGATTTCCCGGGGCAGGGTACGGCTTTGGTAGGAGTGCTCGATGCGTTCTGG 1992 2 1702 .......................................................................................... 1791 0 556 .......................................................................................... 645 E E K G Y I R P E E Y R R F C N S T V P L L R L P K L I Y Query_1 1993 GAAGA-AAAAGGCTACAT-CCGTCCGGAAGAATACCGTCGTTTCTGTAATAGTACGGTACCATTACTGCGCTTGCCGAAGTTGATTTATA 2080 2 1792 .....-............-....................................................................... 1879 2 999 ........ 1006 1 693 ........ 686 0 646 .....N....N.......C..........N............................................................ 735 T N Q E T V K G S L E V A H F G A A P L E V T S T V W T Query_1 2081 CCAACCAGGAAACGGTG-AAAGGAAGTCTGGAAGTGGCACATTTCGG-AGCTGCT-CCGCTGGAGGTGACTTCTA-CTGT-CTGGA-CCC 2164 2 1880 .................-.............................-.......-...................-....-.....-... 1963 2 1007 ... 1009 1 685 ... 683 0 736 ........N........A...............A......N....NNA.......C..N................N....C.....C... 825 L K T K E G K T I A S G T L A H Q P V G I G N C I P L Query_1 2165 TGAAAACAAAAGAA--GGAAAGACAATTGCTTCGGGCA-CGCT-GGCACA-CCAGCCGGTAGGTAT-CGGCAATT-GTATTCCGTT--GG 2245 2 1964 ..............--......................-....-......-...............-........-..........--.. 2044 1 431 ..-......... 441 0 826 ....N.........AG.A..........N.......N.C.N..N......C...N...........C........G.A......N.NG.. 915 0 115 ..-......... 105 G Q L E I P L D K V D V P S C L T L E A T L G D Y A N Query_1 2246 GGCAGCTGG-AGATTCCATTGG-ATAAGGTGGACGTCCC-TTCATGTCTG-ACACTGGAA-GCTACATT--GGGAGATTACGCCAACAG- 2327 2 2045 .........-............-................-..........-.........-........--..................- 2126 0 916 .N..A....A............T...N..N...NN....T...N.N...NA..N......NN..NN...TG..A....NCNC.N...N.N 1005 S W H I W V Y P A A V Q K V A D E A Q L L M T D R L D A Query_1 2328 CTGG-CACAT-CTGGGTATA-TCCT-GCTG-CGGTACAGAAAGTAGCTGATGAAGCACAATTGCTGA--TGACCGACCGTCTGGATGCAA 2410 2 2127 ....-.....-.........-....-....-....................................--..................... 2209 0 1006 ....T.N.N.C.....A...T....G.N.NC..N.T..A..NTA.C...NA...A.C..........ANNNC..NT..CC...N.ACN.. 1095 K A L Q R L Q E G G N V L L S L R K G S L P A E A G G E V V Query_1 2411 AAGCTTTGCAACGTCTTCAGGAAGGTGGCAACGTACTGCTTTCTTTACGGAAAGGCTCCTTGCCTGCCGAAGCGGGAGGCGAAGTAGTGA 2500 2 2210 .......................................................................................... 2299 0 1096 .NCN...C....TN.....N...A.GN..C..N.T.NNN..NN.....-A.....TN....-..... 1160 I G F S S I F W N T A W T L G Q A P H T L G I L C N P A H P Query_1 2501 TAGGTTTCTCTAGCATCTTCTGGAACACGGCCTGGACGCTGGGACAAGCACCGCACACACTGGGTATCCTGTGTAACCCCGCTCATCCGG 2590 2 2300 .......................................................................................... 2389 A L S E F P T E Y Y S D Y Q W W D A M S H S G A I E V V K I Query_1 2591 CACTTTCAGAGTTCCCTACAGAGTATTACAGTGATTATCAGTGGTGGGATGCCATGAGCCATTCCGGTGCCATCGAAGTGGTCAAGATTG 2680 2 2390 .......................................................................................... 2479 D K N L Q P I V R V I D D W F T N R P L A L L F E V K V G K Query_1 2681 ATAAAAACTTGCAGCCGATTGTACGAGTTATCGACGACTGGTTTACGAACCGTCCGCTGGCTTTGTTGTTCGAAGTGAAGGTGGGTAAGG 2770 2 2480 .......................................................................................... 2569 G K L L V S G I D F W Q D M D K R T E A R Q L L Y S L K K Y Query_1 2771 GTAAATTGCTTGTGTCAGGAATTGATTTCTGGCAGGATATGGACAAGCGTACGGAAGCCCGTCAGTTACTCTACAGCTTGAAGAAATATA 2860 2 2570 .......................................................................................... 2659 M C G N R F N P S S E V D A K D L S I L F S I K N Q K Query_1 2861 TGTGCGGTAATCGCTTCAATCCCTCTTCTGAAGTCGATGCGAAAGATTTAAGTATTTTGTTTTCCATTAAAAATCAAAAA 2940 2 2660 ................................................................................ 2739 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 16611564 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 5,368 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200137.114 (CMR200137.19-931.P(931).Y503F.pMCSG68)
QTDKIDLAGSWTFSTDSMDWSRVIELPGSMASNGFGEDIAVGTDWTGGIVDSSYFFKPSYAKYREAGNIKVPFWLQPVKYYKGKAWYQKEVVIPDSWEGKDISLFLERCHWESRLYIDGKEIGMQNALGAPHRYDLTGKLSAGKHVLMLCVDNRVKNIDPGENSHSISDHTQGNWNGVVGDMFLEVKPEVNVSSVKIMPERLAKKVSVSASLMNRYEKDANVVLEMTVGNEKVQQQCTLKPGENQVMMSLAMKGDIKCWDEFSPSLYDLKLSVKDADSGETDVYAERFGFRDVKVKDGKLTINDRRLFLRGTLDCAVFPKTGFPPTDVESWKKIYTTCRQHGLNHVRFHSWCPPEAAFAAADGMGMYLEIECSSWANQSTTIGDGGDLDRFIWEESERIVREFGNHPSFCMMMYGNEPAGEGSNAYLTNFVTTWKERDARRLYCSGAGWPNLPVNDFLSDSNPRIQAWGQGVKSIINAQAPRTDYDWSEYIGRFQQPMVSHEIGQWCVYPNFKEMAKYDGVMRPRNFEIFQETLAENGMAHLADSFLLASGKLQALCYKADIEAALRTKDFGGFQLLGLSDFPGQGTALVGVLDAFWEEKGYIRPEEYRRFCNSTVPLLRLPKLIYTNQETVKGSLEVAHFGAAPLEVTSTVWTLKTKEGKTIASGTLAHQPVGIGNCIPLGQLEIPLDKVDVPSCLTLEATLGDYANSWHIWVYPAAVQKVADEAQLLMTDRLDAKALQRLQEGGNVLLSLRKGSLPAEAGGEVVIGFSSIFWNTAWTLGQAPHTLGILCNPAHPALSEFPTEYYSDYQWWDAMSHSGAIEVVKIDKNLQPIVRVIDDWFTNRPLALLFEVKVGKGKLLVSGIDFWQDMDKRTEARQLLYSLKKYMCGNRFNPSSEVDAKDLSILFSIKNQK
CAGACTGATAAGATTGACCTGGCCGGCTCGTGGACATTTTCTACGGACAGCATGGACTGGAGCCGGGTGATTGAACTGCCGGGTTCAATGGCTTCCAATGGTTTTGGGGAAGATATTGCCGTGGGTACTGATTGGACGGGCGGTATTGTGGATTCTTCTTATTTCTTTAAACCTTCGTATGCCAAATACCGTGAGGCAGGAAATATCAAGGTACCTTTCTGGCTTCAGCCGGTAAAATATTACAAGGGTAAGGCGTGGTATCAGAAAGAGGTGGTGATTCCGGACAGTTGGGAAGGAAAGGACATTTCTCTCTTTTTGGAACGATGCCATTGGGAGAGCCGTTTGTATATAGACGGAAAGGAAATCGGCATGCAAAATGCTTTGGGGGCGCCCCATCGTTATGACCTGACAGGCAAGCTTTCAGCAGGGAAACATGTGTTGATGCTGTGTGTAGACAATCGGGTGAAAAACATTGATCCGGGGGAGAACTCACATAGTATTTCCGACCATACACAAGGAAACTGGAACGGGGTGGTAGGCGATATGTTCCTGGAAGTAAAGCCGGAAGTGAATGTGTCTTCCGTCAAGATTATGCCGGAGCGTCTGGCTAAGAAAGTCAGTGTGTCGGCTTCCTTGATGAACCGTTATGAAAAAGATGCCAATGTGGTACTGGAGATGACGGTAGGTAATGAAAAAGTACAGCAACAATGTACGTTGAAGCCGGGCGAAAATCAAGTGATGATGTCGCTGGCCATGAAGGGAGACATTAAGTGCTGGGATGAGTTTTCTCCATCCTTATATGATTTGAAGCTGAGTGTGAAGGATGCGGATAGCGGTGAAACGGATGTCTATGCGGAACGTTTTGGTTTCCGTGATGTGAAGGTGAAAGACGGCAAACTCACCATCAACGACCGCCGTTTGTTCCTGCGTGGTACGCTGGATTGTGCCGTATTTCCGAAGACCGGTTTCCCGCCCACGGATGTAGAATCCTGGAAAAAGATTTATACCACCTGTCGGCAGCACGGACTGAACCATGTGCGCTTCCATTCCTGGTGTCCGCCCGAAGCTGCTTTTGCAGCTGCCGATGGGATGGGTATGTACCTGGAGATAGAATGTTCTTCCTGGGCTAACCAGTCGACTACCATTGGCGATGGAGGCGATCTGGACCGCTTTATCTGGGAGGAAAGTGAACGCATCGTCCGTGAGTTTGGTAACCATCCTTCTTTCTGCATGATGATGTACGGTAACGAACCGGCTGGTGAGGGAAGTAATGCCTATCTGACTAATTTTGTTACTACCTGGAAAGAGCGCGATGCCCGCCGTTTATATTGTTCGGGTGCCGGATGGCCCAATTTGCCGGTTAACGACTTCTTGAGCGATTCCAATCCTCGTATTCAGGCGTGGGGACAAGGTGTGAAGAGTATTATCAACGCACAGGCTCCGCGTACCGACTATGACTGGTCAGAATACATCGGACGTTTCCAGCAGCCGATGGTGAGCCACGAAATCGGGCAGTGGTGTGTATATCCCAACTTCAAGGAAATGGCCAAATACGACGGGGTGATGCGCCCGCGTAATTTTGAGATATTCCAGGAAACACTGGCTGAAAACGGTATGGCACATTTGGCTGACAGCTTCCTGCTGGCTTCCGGAAAATTGCAGGCGTTGTGTTATAAGGCCGATATCGAAGCTGCTTTGCGTACAAAAGACTTCGGTGGATTCCAGTTACTGGGCTTGTCTGATTTCCCGGGGCAGGGTACGGCTTTGGTAGGAGTGCTCGATGCGTTCTGGGAAGAAAAAGGCTACATCCGTCCGGAAGAATACCGTCGTTTCTGTAATAGTACGGTACCATTACTGCGCTTGCCGAAGTTGATTTATACCAACCAGGAAACGGTGAAAGGAAGTCTGGAAGTGGCACATTTCGGAGCTGCTCCGCTGGAGGTGACTTCTACTGTCTGGACCCTGAAAACAAAAGAAGGAAAGACAATTGCTTCGGGCACGCTGGCACACCAGCCGGTAGGTATCGGCAATTGTATTCCGTTGGGGCAGCTGGAGATTCCATTGGATAAGGTGGACGTCCCTTCATGTCTGACACTGGAAGCTACATTGGGAGATTACGCCAACAGCTGGCACATCTGGGTATATCCTGCTGCGGTACAGAAAGTAGCTGATGAAGCACAATTGCTGATGACCGACCGTCTGGATGCAAAAGCTTTGCAACGTCTTCAGGAAGGTGGCAACGTACTGCTTTCTTTACGGAAAGGCTCCTTGCCTGCCGAAGCGGGAGGCGAAGTAGTGATAGGTTTCTCTAGCATCTTCTGGAACACGGCCTGGACGCTGGGACAAGCACCGCACACACTGGGTATCCTGTGTAACCCCGCTCATCCGGCACTTTCAGAGTTCCCTACAGAGTATTACAGTGATTATCAGTGGTGGGATGCCATGAGCCATTCCGGTGCCATCGAAGTGGTCAAGATTGATAAAAACTTGCAGCCGATTGTACGAGTTATCGACGACTGGTTTACGAACCGTCCGCTGGCTTTGTTGTTCGAAGTGAAGGTGGGTAAGGGTAAATTGCTTGTGTCAGGAATTGATTTCTGGCAGGATATGGACAAGCGTACGGAAGCCCGTCAGTTACTCTACAGCTTGAAGAAATATATGTGCGGTAATCGCTTCAATCCCTCTTCTGAAGTCGATGCGAAAGATTTAAGTATTTTGTTTTCCATTAAAAATCAAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,004 total letters Query= CMR200137.114 Length=3144 Score E Sequences producing significant alignments: (Bits) Value wt 4935 0.0 12E_E9_CMR200137.114_E09.seq 22.9 1.6 Q T D K I D L A G S W T F S T D S M D W S R V I E L P G S M Query_1 202 CAGACTGATAAGATTGACCTGGCCGGCTCGTGGACATTTTCTACGGACAGCATGGACTGGAGCCGGGTGATTGAACTGCCGGGTTCAATG 291 2 1 .......................................................................................... 90 A S N G F G E D I A V G T D W T G G I V D S S Y F F K P S Y Query_1 292 GCTTCCAATGGTTTTGGGGAAGATATTGCCGTGGGTACTGATTGGACGGGCGGTATTGTGGATTCTTCTTATTTCTTTAAACCTTCGTAT 381 2 91 .......................................................................................... 180 A K Y R E A G N I K V P F W L Q P V K Y Y K G K A W Y Q K E Query_1 382 GCCAAATACCGTGAGGCAGGAAATATCAAGGTACCTTTCTGGCTTCAGCCGGTAAAATATTACAAGGGTAAGGCGTGGTATCAGAAAGAG 471 2 181 .......................................................................................... 270 V V I P D S W E G K D I S L F L E R C H W E S R L Y I D G K Query_1 472 GTGGTGATTCCGGACAGTTGGGAAGGAAAGGACATTTCTCTCTTTTTGGAACGATGCCATTGGGAGAGCCGTTTGTATATAGACGGAAAG 561 2 271 .......................................................................................... 360 E I G M Q N A L G A P H R Y D L T G K L S A G K H V L M L C Query_1 562 GAAATCGGCATGCAAAATGCTTTGGGGGCGCCCCATCGTTATGACCTGACAGGCAAGCTTTCAGCAGGGAAACATGTGTTGATGCTGTGT 651 2 361 .......................................................................................... 450 V D N R V K N I D P G E N S H S I S D H T Q G N W N G V V G Query_1 652 GTAGACAATCGGGTGAAAAACATTGATCCGGGGGAGAACTCACATAGTATTTCCGACCATACACAAGGAAACTGGAACGGGGTGGTAGGC 741 2 451 .......................................................................................... 540 1 204 ........... 194 D M F L E V K P E V N V S S V K I M P E R L A K K V S V S A Query_1 742 GATATGTTCCTGGAAGTAAAGCCGGAAGTGAATGTGTCTTCCGTCAAGATTATGCCGGAGCGTCTGGCTAAGAAAGTCAGTGTGTCGGCT 831 2 541 .......................................................................................... 630 1 373 ............ 384 S L M N R Y E K D A N V V L E M T V G N E K V Q Q Q C T L K Query_1 832 TCCTTGATGAACCGTTATGAAAAAGATGCCAATGTGGTACTGGAGATGACGGTAGGTAATGAAAAAGTACAGCAACAATGTACGTTGAAG 921 2 631 .......................................................................................... 720 P G E N Q V M M S L A M K G D I K C W D E F S P S L Y D L K Query_1 922 CCGGGCGAAAATCAAGTGATGATGTCGCTGGCCATGAAGGGAGACATTAAGTGCTGGGATGAGTTTTCTCCATCCTTATATGATTTGAAG 1011 2 721 .......................................................................................... 810 L S V K D A D S G E T D V Y A E R F G F R D V K V K D G K L Query_1 1012 CTGAGTGTGAAGGATGCGGATAGCGGTGAAACGGATGTCTATGCGGAACGTTTTGGTTTCCGTGATGTGAAGGTGAAAGACGGCAAACTC 1101 2 811 .......................................................................................... 900 T I N D R R L F L R G T L D C A V F P K T G F P P T D V E S Query_1 1102 ACCATCAACGACCGCCGTTTGTTCCTGCGTGGTACGCTGGATTGTGCCGTATTTCCGAAGACCGGTTTCCCGCCCACGGATGTAGAATCC 1191 2 901 .......................................................................................... 990 W K K I Y T T C R Q H G L N H V R F H S W C P P E A A F A A Query_1 1192 TGGAAAAAGATTTATACCACCTGTCGGCAGCACGGACTGAACCATGTGCGCTTCCATTCCTGGTGTCCGCCCGAAGCTGCTTTTGCAGCT 1281 2 991 .......................................................................................... 1080 2 1686 ............ 1697 2 1872 ........... 1882 A D G M G M Y L E I E C S S W A N Q S T T I G D G G D L D R Query_1 1282 GCCGATGGGATGGGTATGTACCTGGAGATAGAATGTTCTTCCTGGGCTAACCAGTCGACTACCATTGGCGATGGAGGCGATCTGGACCGC 1371 2 1081 .......................................................................................... 1170 F I W E E S E R I V R E F G N H P S F C M M M Y G N E P A G Query_1 1372 TTTATCTGGGAGGAAAGTGAACGCATCGTCCGTGAGTTTGGTAACCATCCTTCTTTCTGCATGATGATGTACGGTAACGAACCGGCTGGT 1461 2 1171 .......................................................................................... 1260 E G S N A Y L T N F V T T W K E R D A R R L Y C S G A G W P Query_1 1462 GAGGGAAGTAATGCCTATCTGACTAATTTTGTTACTACCTGGAAAGAGCGCGATGCCCGCCGTTTATATTGTTCGGGTGCCGGATGGCCC 1551 2 1261 .......................................................................................... 1350 N L P V N D F L S D S N P R I Q A W G Q G V K S I I N A Q A Query_1 1552 AATTTGCCGGTTAACGACTTCTTGAGCGATTCCAATCCTCGTATTCAGGCGTGGGGACAAGGTGTGAAGAGTATTATCAACGCACAGGCT 1641 2 1351 .......................................................................................... 1440 P R T D F D W S E Y I G R F Q Q P M V S H E I G Q W C V Y P Query_1 1642 CCGCGTACCGACTTTGACTGGTCAGAATACATCGGACGTTTCCAGCAGCCGATGGTGAGCCACGAAATCGGGCAGTGGTGTGTATATCCC 1731 2 1441 .............A............................................................................ 1530 N F K E M A K Y D G V M R P R N F E I F Q E T L A E N G M A Query_1 1732 AACTTCAAGGAAATGGCCAAATACGACGGGGTGATGCGCCCGCGTAATTTTGAGATATTCCAGGAAACACTGGCTGAAAACGGTATGGCA 1821 2 1531 .......................................................................................... 1620 H L A D S F L L A S G K L Q A L C Y K A D I E A A L R T K D Query_1 1822 CATTTGGCTGACAGCTTCCTGCTGGCTTCCGGAAAATTGCAGGCGTTGTGTTATAAGGCCGATATCGAAGCTGCTTTGCGTACAAAAGAC 1911 2 1621 .......................................................................................... 1710 2 1062 ............ 1073 F G G F Q L L G L S D F P G Q G T A L V G V L D A F W E E K Query_1 1912 TTCGGTGGATTCCAGTTACTGGGCTTGTCTGATTTCCCGGGGCAGGGTACGGCTTTGGTAGGAGTGCTCGATGCGTTCTGGGAAGAAAAA 2001 2 1711 .......................................................................................... 1800 G Y I R P E E Y R R F C N S T V P L L R L P K L I Y T N Q E Query_1 2002 GGCTACATCCGTCCGGAAGAATACCGTCGTTTCTGTAATAGTACGGTACCATTACTGCGCTTGCCGAAGTTGATTTATACCAACCAGGAA 2091 2 1801 .......................................................................................... 1890 2 999 ........... 1009 T V K G S L E V A H F G A A P L E V T S T V W T L K T K E G Query_1 2092 ACGGTGAAAGGAAGTCTGGAAGTGGCACATTTCGGAGCTGCTCCGCTGGAGGTGACTTCTACTGTCTGGACCCTGAAAACAAAAGAAGGA 2181 2 1891 .......................................................................................... 1980 K T I A S G T L A H Q P V G I G N C I P L G Q L E I P L D K Query_1 2182 AAGACAATTGCTTCGGGCACGCTGGCACACCAGCCGGTAGGTATCGGCAATTGTATTCCGTTGGGGCAGCTGGAGATTCCATTGGATAAG 2271 2 1981 .......................................................................................... 2070 V D V P S C L T L E A T L G D Y A N S W H I W V Y P A A V Q Query_1 2272 GTGGACGTCCCTTCATGTCTGACACTGGAAGCTACATTGGGAGATTACGCCAACAGCTGGCACATCTGGGTATATCCTGCTGCGGTACAG 2361 2 2071 .......................................................................................... 2160 K V A D E A Q L L M T D R L D A K A L Q R L Q E G G N V L L Query_1 2362 AAAGTAGCTGATGAAGCACAATTGCTGATGACCGACCGTCTGGATGCAAAAGCTTTGCAACGTCTTCAGGAAGGTGGCAACGTACTGCTT 2451 2 2161 .......................................................................................... 2250 S L R K G S L P A E A G G E V V I G F S S I F W N T A W T L Query_1 2452 TCTTTACGGAAAGGCTCCTTGCCTGCCGAAGCGGGAGGCGAAGTAGTGATAGGTTTCTCTAGCATCTTCTGGAACACGGCCTGGACGCTG 2541 2 2251 .......................................................................................... 2340 G Q A P H T L G I L C N P A H P A L S E F P T E Y Y S D Y Q Query_1 2542 GGACAAGCACCGCACACACTGGGTATCCTGTGTAACCCCGCTCATCCGGCACTTTCAGAGTTCCCTACAGAGTATTACAGTGATTATCAG 2631 2 2341 .......................................................................................... 2430 W W D A M S H S G A I E V V K I D K N L Q P I V R V I D D W Query_1 2632 TGGTGGGATGCCATGAGCCATTCCGGTGCCATCGAAGTGGTCAAGATTGATAAAAACTTGCAGCCGATTGTACGAGTTATCGACGACTGG 2721 2 2431 .......................................................................................... 2520 F T N R P L A L L F E V K V G K G K L L V S G I D F W Q D M Query_1 2722 TTTACGAACCGTCCGCTGGCTTTGTTGTTCGAAGTGAAGGTGGGTAAGGGTAAATTGCTTGTGTCAGGAATTGATTTCTGGCAGGATATG 2811 2 2521 .......................................................................................... 2610 D K R T E A R Q L L Y S L K K Y M C G N R F N P S S E V D A Query_1 2812 GACAAGCGTACGGAAGCCCGTCAGTTACTCTACAGCTTGAAGAAATATATGTGCGGTAATCGCTTCAATCCCTCTTCTGAAGTCGATGCG 2901 2 2611 .......................................................................................... 2700 K D L S I L F S I K N Q K Query_1 2902 AAAGATTTAAGTATTTTGTTTTCCATTAAAAATCAAAAA 2940 2 2701 ....................................... 2739 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12361031 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 4,004 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200148.109 (CMR200148.28-761.P(761).E513A.pMCSG68)
KDAEMDRFISDLMGRMTLQEKLGQLNLPAGNDLVSGAVKNSKMAEAIRAGEVGGFFNVKGVDKIYQMQRMAVEETRLGIPLIVGADVIHGYETIFPIPLALSCSWDTAAVTRMARISATEASADGISWTFSPMVDICRDARWGRIAEGSGEDPYLGALMAGAYVRGYQGDGMKQNNEIMACVKHFALYGASESGRDYNSVDMSRNLMYNVYLAPYKGAVEAGVGSVMSSFNTINGVPATADKWLLTDLLRNEWGFTGFVVTDYNSIGEMKTHGVADLKEASARALNAGTDMDMVAHGFLHTLEASLKEKAVTQERIDEACRRVLEAKYKLGLFENPYKYCDTLRGRKELFTEANRKAAREIAAETFVLLKNEGKLLPLQKKGRIALIGPMADAQNNMCGTWNMDCQTDRHVTMYEAFRRAVGDKATVSYAKGSNVYYSEHIEKGAVEPRPLTRGDDRQLRAEALRVAASADVIVAALGESAEMSGESSSRTDIQIPDAQKDLLKALIATGKPVVLALFTGRPLDLCWESEHVPAILNVWFAGSEAGDAIADVMFGEVSPSGKLTTSFPRAVGQLPLYYNHLNTGRPDTDDTTFNRYGSNYIDQSNEPLYPFGYGLSYTTFRYGNLQLSAERMAKGGQLKVTVPVTNSGECDGVEIVQLYLHDVYAEISRPVKELKAFRRVALKKGETQNVEFVLDEDDLKYYNSRLEYGYEPGEFEVMVGPDSRNVQHATFVAE
AAGGATGCGGAGATGGACCGCTTTATCAGTGACCTGATGGGAAGGATGACCTTGCAGGAAAAGTTAGGACAGTTGAATCTGCCGGCTGGGAATGACCTGGTGTCGGGAGCAGTGAAGAACAGCAAGATGGCAGAAGCTATCCGAGCTGGTGAGGTCGGCGGCTTTTTCAATGTGAAGGGAGTGGATAAGATTTACCAGATGCAGCGTATGGCGGTGGAGGAAACTCGTCTGGGAATTCCTTTGATAGTGGGTGCCGATGTGATTCACGGGTACGAAACAATCTTCCCGATTCCGTTGGCCCTGTCTTGTAGCTGGGATACGGCGGCGGTGACACGTATGGCACGTATTTCTGCCACGGAAGCCAGTGCCGATGGAATCAGCTGGACCTTCAGTCCGATGGTAGACATCTGTCGGGATGCCCGCTGGGGACGTATTGCAGAAGGAAGTGGAGAGGACCCGTACCTCGGGGCGTTGATGGCTGGAGCCTATGTGCGCGGTTATCAGGGTGACGGCATGAAGCAGAACAATGAAATCATGGCCTGTGTGAAGCACTTTGCGCTGTATGGAGCTTCGGAATCGGGACGTGACTACAATTCGGTGGATATGAGTCGAAACCTGATGTATAATGTGTACCTGGCTCCTTATAAAGGGGCGGTGGAAGCCGGAGTGGGTTCGGTGATGAGCTCGTTCAATACCATCAACGGGGTACCTGCTACAGCTGACAAATGGCTGCTGACGGATTTGCTCCGCAATGAGTGGGGGTTCACGGGGTTTGTGGTGACCGACTACAATTCGATTGGTGAGATGAAGACTCATGGGGTGGCCGACTTGAAGGAGGCTTCTGCACGGGCGTTGAATGCAGGAACGGACATGGATATGGTGGCACATGGTTTCTTGCATACGCTGGAAGCTTCATTGAAGGAGAAGGCCGTGACGCAGGAGCGGATTGACGAGGCTTGTCGTCGGGTATTGGAAGCCAAGTATAAGTTAGGATTGTTTGAAAATCCTTATAAGTATTGTGATACGCTTCGGGGACGCAAGGAATTGTTTACGGAGGCGAATCGTAAAGCGGCACGTGAGATTGCGGCTGAAACGTTTGTGCTGTTGAAGAACGAGGGTAAGTTGTTGCCTTTGCAGAAAAAAGGACGCATTGCATTGATTGGGCCGATGGCTGATGCGCAGAACAATATGTGCGGCACGTGGAACATGGATTGTCAGACAGACCGTCATGTGACGATGTACGAAGCTTTCCGTCGTGCGGTAGGTGATAAGGCTACGGTTTCTTATGCCAAGGGAAGTAATGTGTATTATAGTGAGCATATTGAGAAAGGGGCGGTCGAACCTCGTCCGCTGACACGTGGCGATGACCGTCAGTTGCGGGCTGAGGCTTTGCGCGTGGCGGCTTCTGCCGATGTGATTGTGGCCGCATTAGGTGAGAGTGCTGAGATGAGCGGAGAGTCTTCTTCTCGTACAGATATTCAGATTCCGGATGCGCAGAAAGATTTGTTGAAGGCATTGATAGCTACCGGAAAGCCGGTGGTACTGGCTTTGTTTACCGGTCGTCCGCTGGATTTATGCTGGGAGTCTGAGCATGTTCCGGCTATCCTGAACGTGTGGTTTGCCGGCAGTGAAGCGGGTGATGCCATTGCCGATGTGATGTTTGGAGAAGTATCTCCTTCGGGTAAGCTGACTACGAGTTTCCCACGTGCGGTGGGACAGTTGCCGCTTTATTATAATCACCTGAATACGGGTCGTCCGGATACGGATGACACTACTTTCAATCGTTATGGCAGCAATTACATCGACCAGAGTAATGAACCGCTTTATCCTTTTGGCTATGGTTTGAGTTATACCACTTTCCGTTACGGTAATTTGCAGTTGAGTGCGGAGCGTATGGCCAAGGGTGGGCAGTTGAAGGTAACCGTGCCTGTAACCAATTCCGGCGAGTGTGACGGAGTAGAGATTGTGCAGTTGTATCTTCACGATGTGTATGCAGAAATCTCCCGTCCGGTGAAGGAGCTGAAAGCTTTCCGCCGTGTGGCCCTTAAAAAGGGAGAGACACAGAATGTAGAGTTTGTACTCGATGAGGATGATTTGAAGTATTATAATTCTCGTCTGGAATATGGATATGAACCGGGAGAGTTTGAAGTGATGGTGGGTCCGGACAGCCGGAATGTGCAGCACGCGACTTTTGTGGCTGAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,884 total letters Query= CMR200148.109 Length=2607 Score E Sequences producing significant alignments: (Bits) Value wt 3963 0.0 09F_F9_CMR200148.109_F09.seq 21.1 5.5 K D A E M D R F I S D L M G R M T L Q E K L G Q L N L P A G Query_1 202 AAGGATGCGGAGATGGACCGCTTTATCAGTGACCTGATGGGAAGGATGACCTTGCAGGAAAAGTTAGGACAGTTGAATCTGCCGGCTGGG 291 2 1 .......................................................................................... 90 2 1814 ...A........... 1828 N D L V S G A V K N S K M A E A I R A G E V G G F F N V K G Query_1 292 AATGACCTGGTGTCGGGAGCAGTGAAGAACAGCAAGATGGCAGAAGCTATCCGAGCTGGTGAGGTCGGCGGCTTTTTCAATGTGAAGGGA 381 2 91 .......................................................................................... 180 V D K I Y Q M Q R M A V E E T R L G I P L I V G A D V I H G Query_1 382 GTGGATAAGATTTACCAGATGCAGCGTATGGCGGTGGAGGAAACTCGTCTGGGAATTCCTTTGATAGTGGGTGCCGATGTGATTCACGGG 471 2 181 .......................................................................................... 270 2 1407 ............. 1419 Y E T I F P I P L A L S C S W D T A A V T R M A R I S A T E Query_1 472 TACGAAACAATCTTCCCGATTCCGTTGGCCCTGTCTTGTAGCTGGGATACGGCGGCGGTGACACGTATGGCACGTATTTCTGCCACGGAA 561 2 271 .......................................................................................... 360 2 582 ........... 572 A S A D G I S W T F S P M V D I C R D A R W G R I A E G S G Query_1 562 GCCAGTGCCGATGGAATCAGCTGGACCTTCAGTCCGATGGTAGACATCTGTCGGGATGCCCGCTGGGGACGTATTGCAGAAGGAAGTGGA 651 2 361 .......................................................................................... 450 E D P Y L G A L M A G A Y V R G Y Q G D G M K Q N N E I M A Query_1 652 GAGGACCCGTACCTCGGGGCGTTGATGGCTGGAGCCTATGTGCGCGGTTATCAGGGTGACGGCATGAAGCAGAACAATGAAATCATGGCC 741 2 451 .......................................................................................... 540 C V K H F A L Y G A S E S G R D Y N S V D M S R N L M Y N V Query_1 742 TGTGTGAAGCACTTTGCGCTGTATGGAGCTTCGGAATCGGGACGTGACTACAATTCGGTGGATATGAGTCGAAACCTGATGTATAATGTG 831 2 541 .......................................................................................... 630 2 784 ............ 795 Y L A P Y K G A V E A G V G S V M S S F N T I N G V P A T A Query_1 832 TACCTGGCTCCTTATAAAGGGGCGGTGGAAGCCGGAGTGGGTTCGGTGATGAGCTCGTTCAATACCATCAACGGGGTACCTGCTACAGCT 921 2 631 .......................................................................................... 720 2 1327 ...........C... 1341 D K W L L T D L L R N E W G F T G F V V T D Y N S I G E M K Query_1 922 GACAAATGGCTGCTGACGGATTTGCTCCGCAATGAGTGGGGGTTCACGGGGTTTGTGGTGACCGACTACAATTCGATTGGTGAGATGAAG 1011 2 721 .......................................................................................... 810 2 586 ............ 597 T H G V A D L K E A S A R A L N A G T D M D M V A H G F L H Query_1 1012 ACTCATGGGGTGGCCGACTTGAAGGAGGCTTCTGCACGGGCGTTGAATGCAGGAACGGACATGGATATGGTGGCACATGGTTTCTTGCAT 1101 2 811 .......................................................................................... 900 T L E A S L K E K A V T Q E R I D E A C R R V L E A K Y K L Query_1 1102 ACGCTGGAAGCTTCATTGAAGGAGAAGGCCGTGACGCAGGAGCGGATTGACGAGGCTTGTCGTCGGGTATTGGAAGCCAAGTATAAGTTA 1191 2 901 .......................................................................................... 990 G L F E N P Y K Y C D T L R G R K E L F T E A N R K A A R E Query_1 1192 GGATTGTTTGAAAATCCTTATAAGTATTGTGATACGCTTCGGGGACGCAAGGAATTGTTTACGGAGGCGAATCGTAAAGCGGCACGTGAG 1281 2 991 .......................................................................................... 1080 I A A E T F V L L K N E G K L L P L Q K K G R I A L I G P M Query_1 1282 ATTGCGGCTGAAACGTTTGTGCTGTTGAAGAACGAGGGTAAGTTGTTGCCTTTGCAGAAAAAAGGACGCATTGCATTGATTGGGCCGATG 1371 2 1081 .......................................................................................... 1170 0 790 ........... 800 A D A Q N N M C G T W N M D C Q T D R H V T M Y E A F R R A Query_1 1372 GCTGATGCGCAGAACAATATGTGCGGCACGTGGAACATGGATTGTCAGACAGACCGTCATGTGACGATGTACGAAGCTTTCCGTCGTGCG 1461 2 1171 .......................................................................................... 1260 2 1489 ...........AG..T... 1507 V G D K A T V S Y A K G S N V Y Y S E H I E K G A V E P R P Query_1 1462 GTAGGTGATAAGGCTACGGTTTCTTATGCCAAGGGAAGTAATGTGTATTATAGTGAGCATATTGAGAAAGGGGCGGTCGAACCTCGTCCG 1551 2 1261 .......................................................................................... 1350 2 646 ...........G... 660 L T R G D D R Q L R A E A L R V A A S A D V I V A A L G E S Query_1 1552 CTGACACGTGGCGATGACCGTCAGTTGCGGGCTGAGGCTTTGCGCGTGGCGGCTTCTGCCGATGTGATTGTGGCCGCATTAGGTGAGAGT 1641 2 1351 .......................................................................................... 1440 2 252 ............. 264 A E M S G A S S S R T D I Q I P D A Q K D L L K A L I A T G Query_1 1642 GCTGAGATGAGCGGAGCTTCTTCTTCTCGTACAGATATTCAGATTCCGGATGCGCAGAAAGATTTGTTGAAGGCATTGATAGCTACCGGA 1731 2 1441 ................AG........................................................................ 1530 2 1174 ...........CA..A... 1192 K P V V L A L F T G R P L D L C W E S E H V P A I L N V W F Query_1 1732 AAGCCGGTGGTACTGGCTTTGTTTACCGGTCGTCCGCTGGATTTATGCTGGGAGTCTGAGCATGTTCCGGCTATCCTGAACGTGTGGTTT 1821 2 1531 .......................................................................................... 1620 A G S E A G D A I A D V M F G E V S P S G K L T T S F P R A Query_1 1822 GCCGGCAGTGAAGCGGGTGATGCCATTGCCGATGTGATGTTTGGAGAAGTATCTCCTTCGGGTAAGCTGACTACGAGTTTCCCACGTGCG 1911 2 1621 .......................................................................................... 1710 2 252 ............ 263 V G Q L P L Y Y N H L N T G R P D T D D T T F N R Y G S N Y Query_1 1912 GTGGGACAGTTGCCGCTTTATTATAATCACCTGAATACGGGTCGTCCGGATACGGATGACACTACTTTCAATCGTTATGGCAGCAATTAC 2001 2 1711 .......................................................................................... 1800 I D Q S N E P L Y P F G Y G L S Y T T F R Y G N L Q L S A E Query_1 2002 ATCGACCAGAGTAATGAACCGCTTTATCCTTTTGGCTATGGTTTGAGTTATACCACTTTCCGTTACGGTAATTTGCAGTTGAGTGCGGAG 2091 2 1801 .......................................................................................... 1890 2 13 ...G........... 27 R M A K G G Q L K V T V P V T N S G E C D G V E I V Q L Y L Query_1 2092 CGTATGGCCAAGGGTGGGCAGTTGAAGGTAACCGTGCCTGTAACCAATTCCGGCGAGTGTGACGGAGTAGAGATTGTGCAGTTGTATCTT 2181 2 1891 .......................................................................................... 1980 H D V Y A E I S R P V K E L K A F R R V A L K K G E T Q N V Query_1 2182 CACGATGTGTATGCAGAAATCTCCCGTCCGGTGAAGGAGCTGAAAGCTTTCCGCCGTGTGGCCCTTAAAAAGGGAGAGACACAGAATGTA 2271 2 1981 .......................................................................................... 2070 E F V L D E D D L K Y Y N S R L E Y G Y E P G E F E V M V G Query_1 2272 GAGTTTGTACTCGATGAGGATGATTTGAAGTATTATAATTCTCGTCTGGAATATGGATATGAACCGGGAGAGTTTGAAGTGATGGTGGGT 2361 2 2071 .......................................................................................... 2160 P D S R N V Q H A T F V A E Query_1 2362 CCGGACAGCCGGAATGTGCAGCACGCGACTTTTGTGGCTGAA 2403 2 2161 .......................................... 2202 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12517470 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:07 PM Number of letters in database: 4,884 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2