Clone ID: IDP00073.112 (IDP00073.1-182.F..pMCSG68)
MSKQLAAQVPAEPVVLGKMGSSYGIRGWLRVFSSTEDAESIFDYQPWFIQKAGQWQQVQLESWKHHNQDLIIKLKGVDDRDAANLLTNCEIVVDSSQLPALEEGDYYWKDLMGCQVVTAEGYDLGKVIDMMETGSNDVLVIKANLKDAFGIKERLVPFLDGQVIKKVDLATRTIEVDWDPGF
ATGAGCAAGCAACTCGCCGCACAAGTACCCGCTGAACCGGTTGTACTGGGGAAAATGGGTTCTTCATACGGTATCCGTGGTTGGCTCAGAGTGTTTTCCTCCACTGAAGACGCCGAAAGCATTTTTGACTATCAGCCCTGGTTTATCCAGAAGGCGGGTCAGTGGCAGCAGGTACAGCTGGAAAGCTGGAAGCACCACAATCAGGATCTGATCATCAAGCTGAAAGGCGTTGACGATCGAGATGCCGCGAATCTTCTGACCAATTGCGAAATTGTCGTGGATTCCTCGCAACTTCCTGCGCTTGAGGAGGGAGACTACTACTGGAAAGACCTGATGGGCTGCCAGGTAGTCACCGCTGAAGGCTACGATCTCGGTAAAGTTATCGACATGATGGAAACCGGGTCGAATGACGTTCTCGTCATTAAGGCGAACCTGAAAGATGCATTTGGTATCAAGGAACGACTAGTACCGTTCCTCGATGGGCAGGTTATCAAGAAAGTCGATCTCGCTACTCGTACTATCGAAGTAGACTGGGATCCTGGTTTT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 1,837 total letters Query= IDP00073.112 Length=951 Score E Sequences producing significant alignments: (Bits) Value 10C_C4_IDP00073.112_T7term.seq 1094 0.0 04C_C4_IDP00073.112_T7short.seq 1009 0.0 wt 985 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 60 CCCCTC-TAGAAAT-AATTTTGTTT-AACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTG 146 0 22 .....NN.......N..........TN............................................................... 111 W S H P Q F E K G T E N L Y F Q S N A M S K Q L A A Q V P A Query_1 147 GTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGAGCAA-GCAACTCGCCGCACAAGTACCCGCTG 235 1 685 .............A............N..........N.. 646 0 112 ...............................................................-.......................... 200 2 1 ........-.......................... 34 E P V V L G K M G S S Y G I R G W L R V F S S T E D A E S I Query_1 236 AACCGGTTGTACTGGGG-AAAATGGGTTCTTCATACGGTATCCGTGGTTGGCTCAGAGTGTTTTCCTCCACTGAAGACGCCGAAAGCATT 324 1 645 .................N........................................................................ 556 0 201 .................-........................................................................ 289 2 35 .................-........................................................................ 123 F D Y Q P W F I Q K A G Q W Q Q V Q L E S W K H H N Q D L I Query_1 325 TTTGACTATCAGCCCTGGTTTATCCAGAAGGCGGGTCAGTGGCAGCAGGTACAGCTGGAAAGCTGGAAGCACCACAATCAGGATCTGATC 414 1 555 .....N.................................N..............N................................... 466 0 290 .......................................................................................... 379 2 124 .......................................................................................... 213 I K L K G V D D R D A A N L L T N C E I V V D S S Q L P A L Query_1 415 ATCAAGCTGAAAGGCGTTGACGATCGAGATGCCGCGAATCTTCTGACCAATTGCGAAATTGTCGTGGATTCCTCGCAACTTCCTGCGCTT 504 1 465 .N.........................................N.............................N........N....... 376 0 380 ..........N...........................................N......................N............ 469 2 214 .......................................................................................... 303 E E G D Y Y W K D L M G C Q V V T A E G Y D L G K V I D M M Query_1 505 GAGGAGGGAGACTACTACTGGAAAGACCTGATGGGCTGCCAGGTAGTCACCGCTGAAGGCTACGATCTCGGTAAAGTTATCGACATGATG 594 1 375 .......................................................................................... 286 0 470 .......................................................................................... 559 2 304 .......................................................................................... 393 E T G S N D V L V I K A N L K D A F G I K E R L V P F L D G Query_1 595 GAAACCGGGTCGAATGACGTTCTCGTCATTAAGGCGAACCTGAAAGATGCATTTGGTATCAAGGAACGACTAGTACCGTTCCTCGATGGG 684 1 285 .......................................N.................................................. 196 0 560 ...........N................................... 606 2 394 .......................................................................................... 483 Q V I K K V D L A T R T I E V D W D P G F * H W K W I T D P Query_1 685 CAGGTTATCAAGAAAGTCGATCTCGCTACTCGTACTATCGAAGTAGACTGGGATCCTGGTTTTTAACATTGGAAGTGGATAACGGATCCG 774 1 195 ..............................................N................N.......................... 106 2 484 ............................................................... 546 N S S S V D K L A A A L E H H H H H H * D P A A N K A R Query_1 775 AATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCT-AACAAAGCCCGAAA 860 1 105 .......A...N.........AAA...N............................A....G.........NA..........A... 19 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1677312 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 1,837 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP00155.104 (IDP00155.1-575.F..pMCSG68)
MPINKLRKRLGDLLVEEGIVSEAQLEQALNAQKNTGRRLGDTLISLGFLSETQLLNFLAQQLSLPVIDLSRAHVDIDAVPLLPEVHARRLRALVIGRSGDTLRIAMSDPADLFAQEALLNQLPDYGFEFVIAPEKQLVDGFDRYYRRTKEIVSFAEQLHAEHKTNDSFDFEITDSDSDEVTVVKLINSLFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLHEVNIASALVLRLKLMANLDISEKRLPQDGRFNIRVKGQSVDIRMSTMPIQHGESVVMRLLNQSAGVRKLEESGIPPHLLLRLRHQLKRPHGMILVTGPTGSGKTTTLYGALNELNTSGKKIITAEDPVEYRISRINQVQVNPKINLDFSTLLRTFLRQDPDIILVGEMRDQETVEIGLRAALTGHLVLSTLHTNDAVDSALRLIDMGAPGYLVASAVRAVVAQRLVRKVCPDCSGHDEVDEARRQWLVTRFPNQAAAKFTRGRGCQNCNLTGYRGRIGVFEMLELDQPMMDCLRANDAVAFSKAARSNTDYKPLLASAMELALQGIVSLDEVMSLGEGDSSGLVEPIYL
GTGCCAATTAATAAACTGCGTAAACGGCTTGGTGACTTGCTGGTTGAAGAGGGGATTGTGAGCGAAGCGCAGCTTGAACAAGCGCTCAATGCCCAGAAAAATACCGGACGCCGCTTAGGGGATACCTTAATTTCGCTTGGCTTTTTAAGCGAAACCCAGTTGCTGAACTTTTTGGCGCAGCAATTGAGTTTGCCAGTGATCGATCTGAGCCGTGCCCATGTCGATATTGATGCTGTTCCTTTACTGCCTGAAGTTCATGCCCGCCGTTTGCGTGCGCTGGTGATAGGCCGCAGTGGTGATACGCTGCGCATAGCCATGAGTGATCCTGCGGATTTGTTTGCTCAAGAAGCCTTGCTCAACCAGTTGCCTGATTATGGTTTTGAGTTTGTCATCGCCCCTGAGAAGCAGTTGGTCGATGGCTTTGATCGTTACTATCGCCGCACCAAAGAAATTGTCTCGTTTGCCGAGCAATTGCATGCCGAACACAAAACCAATGATAGTTTTGATTTTGAGATCACCGATTCAGATAGCGATGAAGTGACGGTTGTTAAACTGATCAACTCGCTGTTTGAAGATGCGATTCAAGTGGGAGCCTCGGATATACATATTGAGCCGGATGCCAACGTACTGCGTTTGCGCCAACGGATCGATGGGGTATTGCATGAAACCTTGCTCCATGAAGTGAATATCGCCTCAGCCTTAGTGCTGCGCTTAAAATTAATGGCGAATCTGGATATTTCAGAAAAACGCCTTCCGCAAGATGGCCGCTTTAACATCCGTGTAAAAGGACAATCCGTCGATATCCGGATGTCAACCATGCCTATCCAGCACGGTGAATCGGTGGTGATGCGTTTGCTCAACCAATCTGCAGGGGTTAGAAAGCTCGAAGAGTCAGGTATTCCGCCTCATTTATTGCTGCGTTTGCGCCATCAGTTGAAACGCCCTCATGGCATGATTCTGGTGACTGGGCCTACCGGCTCGGGGAAAACCACCACCTTGTATGGCGCACTCAACGAGCTCAATACGTCCGGCAAAAAAATCATTACTGCGGAAGATCCGGTGGAATACCGGATTTCACGGATCAATCAGGTGCAAGTGAACCCGAAAATCAATCTCGATTTCTCGACGTTACTGCGCACTTTCTTGCGCCAAGACCCAGATATTATTTTGGTCGGTGAGATGCGTGACCAAGAGACGGTCGAAATTGGCCTGCGCGCTGCATTAACCGGTCACTTAGTCTTAAGTACTCTGCACACCAATGATGCCGTCGATAGCGCGCTGCGTTTGATTGATATGGGGGCGCCCGGTTATCTGGTTGCCAGTGCGGTACGTGCCGTGGTTGCGCAGCGTTTAGTGCGAAAAGTCTGTCCAGATTGTAGTGGACATGATGAGGTGGATGAAGCGCGTCGCCAATGGCTTGTGACGCGTTTCCCTAATCAAGCTGCCGCCAAATTCACTCGTGGACGCGGCTGCCAGAACTGTAACTTAACCGGTTATCGCGGCCGGATCGGTGTATTTGAAATGCTTGAGCTGGATCAGCCGATGATGGATTGCTTGCGGGCAAATGATGCTGTGGCCTTCTCTAAAGCCGCACGCAGTAACACAGATTATAAACCGCTACTGGCTTCGGCGATGGAGTTAGCGCTACAAGGCATCGTCAGTCTTGATGAAGTGATGTCGCTGGGTGAAGGTGATTCCTCTGGTTTGGTTGAACCGATTTATCTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,012 total letters Query= IDP00155.104 Length=2130 Score E Sequences producing significant alignments: (Bits) Value wt 3112 0.0 06C_C6_IDP00155.104_T7short.seq 1790 0.0 12C_C6_IDP00155.104_T7term.seq 1725 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 58 TTCCCCTCTAGAAATAATTTTGTTTAACTTTAAG-AAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTG 146 2 598 ........... 608 0 18 .............-....................T......G.-..G..... 67 0 665 ........... 675 W S H P Q F E K G T E N L Y F Q S N A V P I N K L R K R L G Query_1 147 GTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGTGCCAATTAATAAACTGCGTAAACGGCTTGGTGA 236 2 1 ................................... 35 0 68 ................................... 102 D L L V E E G I V S E A Q L E Q A L N A Q K N T G R R L G D Query_1 237 CTTGCTGGTTGAAGAGGGGATTGTGAGCGAAGCGCAGCTTGAACAAGCGCTCAATGCCCAGAAAAATACCGGACGCCGCTTAGGGGATAC 326 2 36 .......................................................................................... 125 0 103 .......................................................................................... 192 T L I S L G F L S E T Q L L N F L A Q Q L S L P V I D L S R Query_1 327 CTTAATTTCGCTTGGCTTTTTAAGCGAAACCCAGTTGCTGAACTTTTTGGCGCAGCAATTGAGTTTGCCAGTGATCGATCTGAGCCGTGC 416 2 126 .......................................................................................... 215 0 193 .......................................................................................... 282 A H V D I D A V P L L P E V H A R R L R A L V I G R S G D T Query_1 417 CCATGTCGATATTGATGCTGTTCCTTTACTGCCTGAAGTTCATGCCCGCCGTTTGCGTGCGCTGGTGATAGGCCGCAGTGGTGATACGCT 506 2 216 .......................................................................................... 305 0 283 .......................................................................................... 372 L R I A M S D P A D L F A Q E A L L N Q L P D Y G F E F V I Query_1 507 GCGCATAGCCATGAGTGATCCTGCGGATTTGTTTGCTCAAGAAGCCTTGCTCAACCAGTTGCCTGATTATGGTTTTGAGTTTGTCATCGC 596 2 306 .......................................................................................... 395 2 853 ........... 863 0 373 .......................................................................................... 462 A P E K Q L V D G F D R Y Y R R T K E I V S F A E Q L H A E Query_1 597 CCCTGAGAAGCAGTTGGTCGATGGCTTTGATCGTTACTATCGCCGCACCAAAGAAATTGTCTCGTTTGCCGAGCAATTGCATGCCGAACA 686 2 396 .......................................................................................... 485 0 463 .......................................................................................... 552 H K T N D S F D F E I T D S D S D E V T V V K L I N S L F E Query_1 687 CAAAACCAATGATAGTTTTGATTTTGAGATCACCGATTCAGATAGCGATGAAGTGACGGTTGTTAAACTGATCAACTCGCTGTTTGAAGA 776 2 486 .......................................................................................... 575 0 553 .......................................................................................... 642 D A I Q V G A S D I H I E P D A N V L R L R Q R I D G V L Query_1 777 TGCGATTCAAGTGGGAGCCTCGGATATACATATTGAGCCGGATGCCAACGTACTGCGTTT---GCGCCAACGGA--TCGATGGGGTATTG 861 2 576 ............................................................---...........--.............. 660 2 916 ........---...... 929 0 643 ............................................................---...........--.............. 727 0 980 ........---...... 993 1 1162 ......TGC..........GAT.............. 1127 H E T L L H E V N I A S A L V L R L K L M A N L D I S E Query_1 862 C--ATGAAACCTTGCTCCA-TGAAGTGAA-TATCGCCTCAGCCTTAGTGCTGCGCTTAAAATTAATGGCGAATCTGGATATTTCAGAAAA 947 2 661 .--................-.........-............................................................ 746 0 728 .--................-.........-........................................................-... 812 1 1126 NCN....NC.--....T..N.....N...A...........NNN-..AN..N.............N..G--..N..........N...N. 1042 K R L P Q D G R F N I R V K G Q S V D I R M S T M P I Q H G Query_1 948 ACGCCTTCCGC-AAGATGGCCGCTTTAACATCCGTGTAAAAGGACAATCCGTCGATATCCGGATGTCAACCATGCCTATCCAGCACGG-T 1035 2 747 ...........-............................................................................-. 834 0 813 ...........-............................................................................-. 900 1 1041 T.......N..N....A.................N...........N..-...........-..N.......................A. 954 E S V V M R L L N Q S A G V R K L E E S G I P P H L L L R L Query_1 1036 GAATCGGTGGTGATGCGTTTGCTCAACCAATCTGCAGGGGTTAGAAAGCTCGAAGAGTCAGGTATTCCGCCTCATTTATTGCTGCGTTTG 1125 2 835 .......................................................................................... 924 2 628 ......... 636 2 352 ........... 362 0 901 ........................-...........................-..................................... 988 0 695 ......... 703 0 419 ........... 429 1 953 ...................-.....N.-...N..................N............................-........-. 868 R H Q L K R P H G M I L V T G P T G S G K T T T L Y G A L Query_1 1126 CGCCATCAGTTGAAACGCCCTCATGGCATGATTCTGGTGACTGGGCCTACCGGCTCGGGGAAAACCACCA-CCTTGTATGGCGCACTCAA 1214 2 925 ......................................................................-................... 1013 2 637 ..... 641 0 989 .................-........................-..N...-.....TC...G.........C............NN..... 1075 0 704 ..... 708 1 867 ......................................................................-.T................. 779 N E L N T S G K K I I T A E D P V E Y R I S R I N Q V Q V Query_1 1215 CGAGCT-CAATAC-GTCCGGCaaaaaaaTCATTACT-GCGGAAGATCCGGTGGAATACCGGATTTCACGGATCAATCAGGTGCAAGTGAA 1301 2 1014 ......-......-......................-..................................................... 1100 0 1076 ...N..N......G..T.N.................N...... 1118 1 778 ......-......-......................-..................................................... 692 N P K I N L D F S T L L R T F L R Q D P D I I L V G E M R D Query_1 1302 CCCGAAAATCAATCTCGATTTCTCGACGTTACTGCGCACTTTCTTGCGCCAAGACCCAGATATTATTTTGGTCGGTGAGATGCGTGACCA 1391 2 1101 .......................................................................................... 1190 1 691 .......................................................................................... 602 Q E T V E I G L R A A L T G H L V L S T L H T N D A V D S A Query_1 1392 AGAGACGGTCGAAATTGGCCTGCGCGCTGCATTAACCGGTCACTTAGTCTTAAGTACTCTGCACACCAATGATGCCGTCGATAGCGCGCT 1481 2 1191 .......................................................................................... 1280 1 601 ......................................................................A................... 512 L R L I D M G A P G Y L V A S A V R A V V A Q R L V R K V C Query_1 1482 GCGTTTGATTGATATGGGGGCGCCCGGTTATCTGGTTGCCAGTGCGGTACGTGCCGTGGTTGCGCAGCGTTTAGTGCGAAAAGTCTGTCC 1571 2 1281 .......................................................................................... 1370 1 511 ......................................................T................................... 422 P D C S G H D E V D E A R R Q W L V T R F P N Q A A A K F T Query_1 1572 AGATTGTAGTGGACATGATGAGGTGGATGAAGCGCGTCGCCAATGGCTTGTGACGCGTTTCCCTAATCAAGCTGCCGCCAAATTCACTCG 1661 2 1371 .......................................................................................... 1460 1 421 .......................................................................................... 332 R G R G C Q N C N L T G Y R G R I G V F E M L E L D Q P M M Query_1 1662 TGGACGCGGCTGCCAGAACTGTAACTTAACCGGTTATCGCGGCCGGATCGGTGTATTTGAAATGCTTGAGCTGGATCAGCCGATGATGGA 1751 2 1461 .......................................................................................... 1550 1 331 .......................................................................................... 242 D C L R A N D A V A F S K A A R S N T D Y K P L L A S A M E Query_1 1752 TTGCTTGCGGGCAAATGATGCTGTGGCCTTCTCTAAAGCCGCACGCAGTAACACAGATTATAAACCGCTACTGGCTTCGGCGATGGAGTT 1841 2 1551 .......................................................................................... 1640 1 241 .......................................................................................... 152 L A L Q G I V S L D E V M S L G E G D S S G L V E P I Y L * Query_1 1842 AGCGCTACAAGGCATCGTCAGTCTTGATGAAGTGATGTCGCTGGGTGAAGGTGATTCCTCTGGTTTGGTTGAACCGATTTATCTGTAACA 1931 2 1641 ..................................................................................... 1725 1 151 ......................................................................-..............C.T.. 63 H W K W I T D P N S S S V D K L A A A L E H H H H H H * D P Query_1 1932 TTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGC 2021 1 62 . 62 1 68 ...T..T.....T.....T.A......... 39 A A N K A R K E Query_1 2022 TGCTAACAAAGCCCGAAAGGAA 2043 1 38 ..................A... 17 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 8369593 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 4,012 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP00510.102 (IDP00510.20-207.P(207)..pMCSG68)
VDQSNPYRLMDEAAKKTFTRLKNEQPKIKQNPDYLRTIVREELLPFVQIKYAGALVLGSYYKDATPAQREAYFNAFGKYLEQAYGQALALYHGQTYDVAPDQPLGDANIVAIRVTILDPSGRPPVRLDFQWRKNSQTGNWQAYDMIAEGVSMISTKQNEWASILRQKGVDGLTQQLLSAAKQPITLDK
GTCGATCAAAGCAACCCTTATCGTCTGATGGATGAGGCAGCGAAGAAAACCTTTACGCGTCTGAAAAATGAGCAGCCTAAGATTAAGCAAAATCCAGATTATCTCCGCACCATTGTACGCGAAGAGCTGTTGCCGTTTGTTCAGATCAAATACGCCGGCGCGTTAGTACTGGGTAGCTATTATAAAGATGCTACACCTGCACAGCGTGAAGCGTATTTCAATGCATTCGGTAAATATCTGGAGCAGGCATACGGGCAGGCATTGGCGTTGTATCACGGCCAAACTTACGATGTGGCACCAGACCAGCCTTTAGGGGATGCCAACATCGTTGCTATCCGCGTCACCATTCTCGATCCAAGTGGGCGTCCACCTGTCCGGTTAGATTTCCAATGGCGTAAAAATAGCCAAACCGGTAACTGGCAGGCTTATGACATGATCGCCGAAGGGGTGAGCATGATTAGCACCAAACAGAATGAATGGGCCTCTATCCTGCGCCAAAAGGGTGTGGATGGTTTGACCCAACAATTGCTGAGTGCGGCTAAACAGCCAATCACCTTAGATAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 1,291 total letters Query= IDP00510.102 Length=969 Score E Sequences producing significant alignments: (Bits) Value 10G_G4_IDP00510.102_T7term.seq 1177 0.0 wt 1018 0.0 L Y F Q S N A V D Q S N P Y R L M D E A A K K T F T R L K Query_1 179 ACCTGTACTT-CCAATCCAATGCCGTCGATCAAAGCAACCCTTATCGTCTGATGGATGAGGCAGCGAAGAAAACCTTTACGCGTCTGAAA 267 1 721 ..........T.....N...............................................N.......-................. 633 2 1 .................................................................. 66 N E Q P K I K Q N P D Y L R T I V R E E L L P F V Q I K Y A Query_1 268 AATGAGCAGCCTAAGATTAAGCAAAATCCAGATTATCTCCGCACCATTGTACGCGAAGAGCTGTTGCCGTTTGTTCAGATCAAATACGCC 357 1 632 ..................................................N.........N............................. 543 2 67 .......................................................................................... 156 G A L V L G S Y Y K D A T P A Q R E A Y F N A F G K Y L E Q Query_1 358 GGCGCGTTAGTACTGGGTAGCTATTATAAAGATGCTACACCTGCACAGCGTGAAGCGTATTTCAATGCATTCGGTAAATATCTGGAGCAG 447 1 542 ....................N.....................N............................................... 453 2 157 .......................................................................................... 246 A Y G Q A L A L Y H G Q T Y D V A P D Q P L G D A N I V A I Query_1 448 GCATACGGGCAGGCATTGGCGTTGTATCACGGCCAAACTTACGATGTGGCACCAGACCAGCCTTTAGGGGATGCCAACATCGTTGCTATC 537 1 452 ........N................................................................................. 363 2 247 .......................................................................................... 336 R V T I L D P S G R P P V R L D F Q W R K N S Q T G N W Q A Query_1 538 CGCGTCACCATTCTCGATCCAAGTGGGCGTCCACCTGTCCGGTTAGATTTCCAATGGCGTAAAAATAGCCAAACCGGTAACTGGCAGGCT 627 1 362 ............N..........................N..........N....................................... 273 2 337 .......................................................................................... 426 Y D M I A E G V S M I S T K Q N E W A S I L R Q K G V D G L Query_1 628 TATGACATGATCGCCGAAGGGGTGAGCATGATTAGCACCAAACAGAATGAATGGGCCTCTATCCTGCGCCAAAAGGGTGTGGATGGTTTG 717 1 272 ...............................................N..........N........................N...... 183 2 427 .......................................................................................... 516 T Q Q L L S A A K Q P I T L D K * H W K W I T D P N S S S V Query_1 718 ACCCAACAATTGCTGAGTGCGGCTAAACAGCCAATCACCTTAGATAAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTC 807 1 182 .........................................................................................N 93 2 517 ................................................ 564 D K L A A A L E H H H H H H * D P A A N K Query_1 808 GACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAA 870 1 92 ........N...............................................N...... 30 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1192795 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 1,291 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP01085.102 (IDP01085.1-206.F..pMCSG68)
MATPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIADALEGKHPAVPVDVVHRALREIHERADAVRRERFKAMAAEGVKYLEENREKDGVNSTESGLQFRVLTQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIAGWIEALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL
ATGGCCACCCCGACTTTTGACACTATTGAAGCGCAAGCGAGCTACGGCATTGGTTTGCAGGTAGGGCAGCAGCTCAGCGAATCCGGTCTTGAAGGGCTGTTGCCTGAAGCGCTGGTTGCAGGCATTGCTGATGCGCTGGAAGGTAAACACCCGGCGGTTCCTGTTGATGTGGTGCATCGTGCGCTGCGTGAAATTCATGAGCGTGCGGACGCGGTGCGTCGTGAGCGTTTCAAAGCGATGGCGGCCGAAGGCGTGAAATACCTGGAAGAAAACCGCGAAAAAGACGGTGTGAACAGTACTGAGTCTGGTTTACAGTTCCGCGTTCTGACGCAGGGCGAAGGCGCGATTCCGGCGCGTACCGACCGCGTACGCGTGCATTACACCGGTAAGCTTATTGATGGCACCGTATTTGACAGCTCCGTCGCGCGCGGCGAACCGGCTGAATTCCCGGTAAATGGCGTGATCGCCGGCTGGATTGAAGCGCTGACTCTGATGCCGGTAGGTTCCAAATGGGAACTGACTATCCCACAGGAGCTGGCCTATGGCGAGCGTGGGGCCGGGGCATCTATTCCGCCATTCAGCACCCTGGTGTTTGAAGTGGAACTGCTGGAAATTCTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,540 total letters Query= IDP01085.102 Length=1023 Score E Sequences producing significant alignments: (Bits) Value 10B_B4_IDP01085.102_T7term.seq 1543 0.0 wt 1115 0.0 04B_B4_IDP01085.102_T7short.seq 921 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V Query_1 1 TCTCGA-TCCCGCGAAA-TTAATACGACTCACTAT-AGGGGAATTGTGAGCGGATAACAA--TTCCCCTCTAGAA--ATAATTTTGTTTA 83 1 962 .....NN..........A............C....T.....................AT.AT.............--.W........... 875 1 916 ....--....... 926 0 14 ..N.N--..........N..TA..TT......... 46 * L * E G D I H M H H H H H H S S G V D L W S H P Q F E K Query_1 84 ACTTTAA-GAAGG-AGATATACATAT-GCACCATCATCATCATCATT-CTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAA-G 168 1 874 .......A.....G............-....................T....N................N..................A. 786 0 47 .......-.....-............T....................-........................................-. 132 G T E N L Y F Q S N A M A T P T F D T I E A Q A S Y G I G Query_1 169 GGTACCGAGAACCTGTACTTCCAATCCAA-TGCCATGGCCACCCCGACTTTTGACACTATTGAAGCGCAA-GCGAGCTACGGCATTGGTT 256 1 785 ...N.........................A........................................A................... 696 2 1 ....................................-................... 55 0 133 .............................-........................................-.....T............. 220 L Q V G Q Q L S E S G L E G L L P E A L V A G I A D A L E G Query_1 257 TGCAGGTAGGGCAGCAGCTCAGCGAATCCGGTCTTGAAGGGCTGTTGCCTGAA-GCGCTGGTTGCAGGCATTGCTGATGCGCTGGAAGGT 345 1 695 .....................................................A...................N................ 606 2 56 .....................................................-.................................... 144 0 221 .....................................................-.................................... 309 K H P A V P V D V V H R A L R E I H E R A D A V R R E R F K Query_1 346 AAACACCCGGCGGTTCCTGTTGATGTGGTGCATCGTGCGCTGCGTGAAATTCATGAGCGTGCGGACGCGGTGCGTCGTGAGCGTTTCAAA 435 1 605 .......................................................................................... 516 2 145 .......................................................................................... 234 0 310 .......................A................................N............N......N............. 399 A M A A E G V K Y L E E N R E K D G V N S T E S G L Q F R V Query_1 436 GCGATGGCGGCCGAAGGCGTGAAATACCTGGAAGAAAACCGCGAAAAAGACGGTGTGAACAGTACTGAGTCTGGTTTACAGTTCCGCGTT 525 1 515 ................................................................N......................... 426 2 235 .......................................................................................... 324 0 400 N.........................................N........N...................................... 489 L T Q G E G A I P A R T D R V R V H Y T G K L I D G T V F D Query_1 526 CTGACGCAGGGCGAAGGCGCGATTCCGGCGCGTACCGACCGCGTACGCGTGCATTACACCGGTAAGCTTATTGATGGCACCGTATTTGAC 615 1 425 .......................................................................................... 336 2 325 .......................................................................................... 414 0 490 ........N......N..............................N................................ 568 S S V A R G E P A E F P V N G V I A G W I E A L T L M P V G Query_1 616 AGCTCCGTCGCGCGCGGCGAACCGGCTGAATTCCCGGTAAATGGCGTGATCGCCGGCTGGATTGAAGCGCTGACTCTGATGCCGGTAGGT 705 1 335 .......................................................................................... 246 1 1137 ... 1135 2 415 .......................................................................................... 504 S K W E L T I P Q E L A Y G E R G A G A S I P P F S T L V F Query_1 706 TCCAAATGGGAACTGACTATCCCACAGGAGCTGGCCTATGGCGAGCGTGGGGCCGGGGCATCTATTCCGCCATTCAGCACCCTGGTGTTT 795 1 245 .......................................................................................... 156 1 1134 ........ 1127 2 505 .......................................................................................... 594 E V E L L E I L * H W K W I T D P N S S S V D K L A A A L E Query_1 796 GAAGTGGAACTGCTGGAAATTCTGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAG 885 1 155 ...........................-T.N.......................................C....-.............. 68 2 595 ........................ 618 H H H H H H * D P A A N K A R K E Query_1 886 caccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAA 936 1 67 .......................................N.......N... 17 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2514960 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 2,540 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP01091.102 (IDP01091.1-322.F..pMCSG68)
MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYGRGLDKQRLTQYQTKLGAAMVIVAAWCVEDYQVIRLAGSLTPRATRLAHEAQLDVAPLGKIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEKVAEVTERAMRGELDFTASLRSRVATLKGADADILRQVRGNLPLMPGLTQLVLKLEALGWKIAIASGGFTFFADYLRDQLRLTAAVANELEIMDGKFTGHVIGDIVDAEYKANTLLRLAQEHDIPLAQTVAIGDGANDLPMIKAAGLGIAFHAKPKVNEKTEITIRHADLMGVFCILSGSMNQK
ATGCCTAACATTACCTGGTGCGATCTGCCTGAAGATGTCTCTTTATGGCCTGGTTTGCCTCTTTCTTTAAGCGGCGACGAGGTGATGCCTCTGGATTACCATGCAGGCCGCAGCGGTTGGCTGCTGTATGGGCGCGGGCTGGATAAGCAGCGTTTAACTCAATATCAGACCAAACTGGGCGCCGCAATGGTGATTGTCGCCGCCTGGTGCGTGGAGGATTATCAGGTCATTCGTCTGGCGGGGTCGCTGACGCCGCGTGCGACGCGGCTGGCGCATGAGGCGCAGCTGGATGTCGCGCCGCTGGGTAAAATTCCGCATCTGCGCACGCCGGGGCTGCTGGTGATGGATATGGATTCCACGGCGATCCAAATTGAGTGTATCGATGAAATCGCGAAGCTGGCCGGTACCGGTGAGAAGGTGGCTGAAGTGACGGAGCGCGCGATGCGCGGCGAGCTTGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCGGATGCCGATATTTTGCGACAGGTGCGCGGGAATCTGCCGCTAATGCCAGGATTAACGCAGTTGGTGCTAAAACTGGAAGCGCTCGGCTGGAAAATCGCTATCGCCTCCGGCGGCTTCACTTTCTTCGCCGACTATCTGCGTGACCAGCTACGTCTGACGGCGGCAGTCGCCAATGAACTGGAGATCATGGACGGTAAATTTACCGGCCACGTTATCGGCGATATTGTGGATGCTGAGTATAAAGCCAATACCTTGCTTCGTCTGGCGCAAGAGCATGACATCCCGCTGGCGCAGACGGTGGCGATTGGCGACGGCGCTAACGATCTGCCAATGATCAAAGCGGCAGGGTTAGGTATTGCCTTCCATGCCAAGCCAAAAGTAAACGAAAAGACGGAGATTACGATCCGCCACGCTGACCTGATGGGCGTGTTTTGCATTCTCTCCGGCAGCATGAACCAGAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,504 total letters Query= IDP01091.102 Length=1371 Score E Sequences producing significant alignments: (Bits) Value wt 1743 0.0 12B_B6_IDP01091.102_T7term.seq 1716 0.0 06B_B6_IDP01091.102_T7short.seq 1642 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V Query_1 1 TCTCGAT-CCCGCGAAATTAATACGACTCACTAT-AGGGGAATT-GTGAGCGGAT--AACAATTCCCCTCTAG--AAATAATTTTGTTTA 83 1 1144 .......T...........T.NN......N....T.........N.........ATA...........N...AGA............... 1055 0 19 ...........--............... 44 * L * E G D I H M H H H H H H S S G V D L W S H P Q F E K G Query_1 84 ACTTTAAG-AAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTA 172 1 1054 ..-.....-N.....G.-..G.... 1033 0 45 ........T......G.-..G...... 70 T E N L Y F Q S N A M P N I T W C D L P E D V S L W P G L P Query_1 173 CCGAGAACCTGTACTTCCAATCCAATGCCATGCCTAACATTACCTGGTGCGATCTGCCTGAAGATGTCTCTTTATGGCCTGGTTTGCCTC 262 2 1 ............................................................. 61 1 1032 ............................................................ 973 0 67 ....................N........................................ 127 L S L S G D E V M P L D Y H A G R S G W L L Y G R G L D K Q Query_1 263 TTTCTTTAAGCGGCGACGAGGTGATGCCTCTGGATTACCATGCAGGCCGCAGCGGTTGGCTGCTGTATGGGCGCGGGCTGGATAAGCAGC 352 2 62 .......................................................................................... 151 1 972 ........................................N................................................. 883 0 128 .......................................................................................... 217 R L T Q Y Q T K L G A A M V I V A A W C V E D Y Q V I R L A Query_1 353 GTTTAACTCAATATCAGACC-AAACTGGGCGCCGCAATGGTGATTGTCGCCGCCTGGTGCGTGGAGGATTATCAGGTCATTCGTCTGGCG 441 2 152 ....................-..................................................................... 240 2 761 ........... 771 1 882 ...-................C........................A............................................ 794 1 273 ........... 263 0 218 ....................-........................A............................................ 306 0 829 ........... 839 G S L T P R A T R L A H E A Q L D V A P L G K I P H L R T P Query_1 442 GGGTCGCTGACGCCGCGTGCGACGCGGCTGGCGCATGAGGCGCAGCTGGATGTCGCGCCGCTGGGTAAAATTCCGCATCTGCGCACGCCG 531 2 241 .......................................................................................... 330 1 793 .......................................................................................... 704 0 307 .......................................................................................... 396 G L L V M D M D S T A I Q I E C I D E I A K L A G T G E K V Query_1 532 GGGCTGCTGGTGATGGATATGGATTCCACGGCGATCCAAATTGAGTGTATCGATGAAATCGCGAAGCTGGCCGGTACCGGTGAGAAGGTG 621 2 331 .......................................................................................... 420 1 703 .......................................................................................... 614 0 397 .......................................................................................... 486 A E V T E R A M R G E L D F T A S L R S R V A T L K G A D A Query_1 622 GCTGAAGTGACGGAGCGCGCGATGCGCGGCGAGCTTGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCGGATGCC 711 2 421 .......................................................................................... 510 1 613 .......................................................................................... 524 0 487 .......................................................................................... 576 D I L R Q V R G N L P L M P G L T Q L V L K L E A L G W K I Query_1 712 GATATTTTGCGACAGGTGCGCGGGAATCTGCCGCTAATGCCAGGATTAACGCAGTTGGTGCTAAAACTGGAAGCGCTCGGCTGGAAAATC 801 2 511 .......................................................................................... 600 1 523 .......................................................................................... 434 0 577 .......................................................................................... 666 A I A S G G F T F F A D Y L R D Q L R L T A A V A N E L E I Query_1 802 GCTATCGCCTCCGGCGGCTTCACTTTCTTCGCCGACTATCTGCGTGACCAGCTACGTCTGACGGCGGCAGTCGCCAATGAACTGGAGATC 891 2 601 .......................................................................................... 690 1 433 .......................................................................................... 344 0 667 .......................................................................................... 756 M D G K F T G H V I G D I V D A E Y K A N T L L R L A Q E Query_1 892 ATGGACGGTAAA-TTTACCGGCCACGTTATCGGCGATATTGTGGATGCTGAGTATAAAGCCAATACC-TTGCTTCGTCTGGCGCAAGAGC 979 2 691 ............-......................................................-...................... 778 2 713 ...C.......-.......T... 692 2 230 ........... 240 1 343 ............-......................................................-...................... 256 1 321 ...C.......-.......T... 342 1 804 ........... 794 0 757 ............T......................................................T...................... 846 0 296 ........... 306 H D I P L A Q T V A I G D G A N D L P M I K A A G L G I A Query_1 980 ATGACATCCCGCTGGCG-CAGACGGTGGCGATTGGCGACGGCGCTAACGATCTG-CCAATGATCAAAGCGGCAGGGTTAGGTATTGCC-T 1066 2 779 .................-....................................-.................................-. 865 1 255 .................-....................................-.................................-. 169 0 847 .................C...........................T........C...N.............................T. 936 F H A K P K V N E K T E I T I R H A D L M G V F C I L S Query_1 1067 TCCATGCCAAGCCAAAAGTAAACGAAAAG-ACGGAGATTACGA-TCCGCCACGCTG-ACCTGATGGG-CGTG-TTTTGC-ATTC-TCTCC 1149 2 866 .............................-.............-............-..........-....-......-....-..... 948 1 168 .............................-.............-............-..........-....-......-....-....N 86 0 937 ........N....................A.............T............A..........C....T......A....T....N 1026 G S M N Q K * H W K W I T D P N S S S V D K L A A A L E H H Query_1 1150 GGCAGCATGAACCAGAAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccac 1239 2 949 .................. 966 1 85 ..................C.T... 62 1 67 ..T..T 62 0 1027 ................. 1043 H H H H * D P A A N K A R K E A E L A A Query_1 1240 caccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG 1300 2 116 ........... 126 1 61 .....T.....T.A........................... 21 1 918 ........... 908 0 182 ........... 192 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4682880 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 3,504 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP02332.102 (IDP02332.1-258.F..pMCSG68)
MPINFHDANNKYTYAQRNAHISWGEMIKGITNIQNKQIIDIGCGGGIYTKELALMGAKSVVGLDFSKEILQAAKENCSGFSNISFIHGDAHSTPYPNDTFDIVISRAVIHHLQDIPTFLREASRILKKNGVLIVQDRTIEDCTIPGSPEHIRGYFFSIFPKLIEIESKRRPKTTTIQKELQKYFLHVFPTQTQWEVRKIHDSVEALLQDLSPRTGRSILYELTDYELSQLLQQVQTALQNVSPIIERDRWTIWSAMKL
ATGCCCATTAATTTTCACGATGCAAATAATAAATACACATACGCACAGCGAAATGCCCATATTTCTTGGGGAGAAATGATAAAAGGTATTACAAATATACAAAACAAGCAAATAATTGATATTGGCTGTGGTGGCGGGATTTATACGAAAGAACTTGCTCTTATGGGAGCAAAAAGTGTTGTTGGGCTTGATTTTTCAAAAGAAATATTACAGGCCGCAAAAGAAAATTGTAGTGGATTTTCAAATATTTCATTCATTCACGGTGATGCACATAGTACCCCATATCCTAATGATACATTCGACATTGTTATTTCGCGTGCGGTTATTCATCATTTGCAAGACATTCCTACATTTCTACGTGAAGCTTCTCGTATATTAAAGAAAAACGGTGTACTTATTGTACAAGACCGAACCATTGAAGATTGTACAATTCCTGGAAGCCCTGAACACATTCGTGGATATTTTTTCTCTATATTTCCGAAACTTATCGAAATAGAATCAAAAAGACGACCAAAAACCACCACAATACAAAAAGAATTACAAAAATATTTTCTTCACGTGTTCCCTACTCAAACACAATGGGAAGTACGAAAAATACATGATTCTGTAGAAGCATTATTGCAAGATTTATCCCCCCGAACAGGTCGATCCATTCTATACGAATTAACAGATTATGAACTTTCTCAACTTCTACAACAAGTACAGACTGCCTTACAAAACGTCTCTCCTATTATCGAAAGGGATCGGTGGACCATTTGGAGCGCCATGAAATTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,745 total letters Query= IDP02332.102 Length=1179 Score E Sequences producing significant alignments: (Bits) Value wt 1397 0.0 11F_F5_IDP02332.102_T7term.seq 1379 0.0 05F_F5_IDP02332.102_T7short.seq 1225 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAG-AAATAATTTTGTTTAACTTTA 89 1 949 ....................................................................A..................... 860 1 882 ...G...........T.T.C.C.C...........A... 920 0 21 ........N-..................... 50 * E G D I H M H H H H H H S S G V D L W S H P Q F E K G T Query_1 90 A--G-AAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGA 176 1 859 .--.T......G.-..G......-.... 836 0 51 .AGT-......G.-..G......-.... 75 E N L Y F Q S N A M P I N F H D A N N K Y T Y A Q R N A H I Query_1 177 GAACCTGTACTTCCAATCCAATGCCATGCCCATTAATTTTCACGATGCAAATAATAAATACACATACGCACAGCGAAATGCCCATATTTC 266 2 1 ................................................................. 65 1 843 ................................................................. 779 0 68 ........................N........................................ 132 S W G E M I K G I T N I Q N K Q I I D I G C G G G I Y T K E Query_1 267 TTGGGGAGAAATGATAAAAGG-TATTACAAATATACAAAACAAGCAAATAATTGATATTGGCTGTGGTGGCGGG-ATTTATACGAAAGAA 354 2 66 .....................-....................................................-............... 153 1 778 .....................G....................................................G............... 689 0 133 .....................-....................................................-............... 220 L A L M G A K S V V G L D F S K E I L Q A A K E N C S G F S Query_1 355 CTTGCTCTTATGGGAGCAAAAAGTGTTGTTGGGCTTGATTTTTCAAAAGAAATATTACAGGCCGCAAAAGAAAATTGTAGTGGATTTTCA 444 2 154 .......................................................................................... 243 1 688 .......................................................................................... 599 0 221 .......................................................................................... 310 N I S F I H G D A H S T P Y P N D T F D I V I S R A V I H H Query_1 445 AATATTTCATTCATTCACGGTGATGCACATAGTACCCCATATCCTAATGATACATTCGACATTGTTATTTCGCGTGCGGTTATTCATCAT 534 2 244 .......................................................................................... 333 1 598 .......................................................................................... 509 0 311 .......................................................................................... 400 L Q D I P T F L R E A S R I L K K N G V L I V Q D R T I E D Query_1 535 TTGCAAGACATTCCTACATTTCTACGTGAAGCTTCTCGTATATTAAAGAAAAACGGTGTACTTATTGTACAAGACCGAACCATTGAAGAT 624 2 334 .......................................................................................... 423 1 508 .......................................................................................... 419 0 401 ........................................................................N................. 490 C T I P G S P E H I R G Y F F S I F P K L I E I E S K R R P Query_1 625 TGTACAATTCCTGGAAGCCCTGAACACATTCGTGGATATTTTTTCTCTATATTTCCGAAACTTATCGaaatagaatcaaaaagacgacca 714 2 424 .......................................................................................... 513 1 418 .......................................................................................... 329 0 491 .......................................................................................... 580 K T T T I Q K E L Q K Y F L H V F P T Q T Q W E V R K I H Query_1 715 aaaaccaccacaatacaaaaagaattacaaaaatATTTTCTTCACGTGTTCCCTACTCAAACACAATGGGAAGTACGAAAAATACATGA- 803 2 514 .........................................................................................- 602 1 328 .........................................................................................- 240 0 581 .........................................................................................T 670 D S V E A L L Q D L S P R T G R S I L Y E L T D Y E L S Q Query_1 804 TTCTGTAGAAGCATTATTGCAAGATTTATCCCCCCGAACAGGTCGATCCA-TTCTATACG-AATTAACAGATTATGAACTTTCTCAAC-T 890 2 603 ..................................................-.........-...........................-. 689 1 239 ..................................................-.........-...........................-. 153 0 671 ..................................................T........NA...........................T. 760 L L Q Q V Q T A L Q N V S P I I E R D R W T I W S A M K L * Query_1 891 TCTACAACAAGTACAGACTGCCTTACAAAACGTCTCTCCTATTATCGAAAGGGATCGGTGGACCATTTGGAGCGCCATGAAATTGTAACA 980 2 690 ..................................................................................... 774 1 152 .N.................N.................................................................C.T.. 63 0 761 .......... 770 H W K W I T D P N S S S V D K L A A A L E H H H H H H * D P Query_1 981 TTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGC 1070 1 62 . 62 1 68 ...T..T.....T.....T.A......... 39 A A N K A R K E Query_1 1071 TGCTAACAAAGCC-CGAAAGGAA 1092 1 38 ....-...G....A.....A... 17 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3136611 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 2,745 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP02374.103 (IDP02374.1-868.F..pMCSG73)
MSSFVLDFQEIEKTQLSLVGGKGLNLGALSNMQGIQVPEGFCVTTAGYEKAIEQNEELQALLQQLTKLKREERVRIGEISKKIRENIMAVQIPSDVVEAVAHYLSRFGNEHAYAVRSSATAEDLPYASFAGQQDTYLNIIGENAILQHVRKCWASLFTDRAVMYRMQNGFEHNQVSICVVVQKMVFPQASGILFTADPITSNRKVLSIDASFGLGEALVSGLVSADNYKVKEGEIIDKMIATKKLAIYALKEGGTETKQNDPVQQKIQTLSDQQILQLVQIGRQIEAYFGCPQDIEWCLVDHTFYIVQSRPITTLYPIPEENDEGNHVYISVGHQQMMTDAMKPLGLSFFLLTTNAPMRKAGGRLFVDATQQLVLPASRDYLINTLGKSDPLVRDALTTIIERDDFITLLPDEEKEKSASKSKPPVSSQPEIENDPAIVTELIRNSEASLEELKQNMQLKSGVDVFDFILEDIQQLKKVLFNPQSIAVIMAGMNASTWINEKMEQWLGEKNAADMLSQSVQNNITSEMGLALMDVADAIRPYPEVITYLQHVEDDSFLDELVQYKGGEKARDTIVAFLNKYGMRCSGEIDITKTRWSEKPTTIIPMILNNIRDFEAGASKQKFEKGLQEALNKEEELLDRLQHLPKGKQKVEETKRMIRNIRNFIGYREYPKYGMINRYFIYKQALLKEAEQLVQSGVICEVDDIYYLTFEELHEVVRTNKLDYELIQKQKNDYKLYEKLTPPRVMTSDGEIITGKYKRENLPADAIVGLPVSSGVVEGRARVILNMEEANLEEGDILVTAFTDPGWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENATKRIKDGQRIRVHGTEGYIEML
ATGAGTTCTTTCGTTCTCGATTTTCAGGAAATAGAAAAAACGCAGCTTTCACTCGTTGGTGGAAAAGGTTTGAATTTAGGGGCGTTATCCAACATGCAAGGGATACAAGTGCCAGAAGGTTTTTGTGTTACAACGGCAGGATATGAAAAGGCCATCGAGCAAAATGAGGAGCTTCAAGCTTTGTTGCAACAGCTAACAAAGTTGAAACGTGAAGAGCGAGTTCGAATTGGTGAAATCAGCAAGAAGATTAGAGAAAACATTATGGCGGTACAAATCCCTTCTGATGTAGTGGAAGCAGTAGCTCATTATCTCTCACGCTTTGGAAATGAACATGCGTATGCCGTACGCTCTAGTGCGACGGCTGAAGATTTACCGTATGCCTCGTTTGCTGGTCAACAAGATACGTATTTAAATATTATCGGAGAAAACGCAATTTTACAGCATGTAAGAAAGTGCTGGGCTTCTTTATTTACAGATCGTGCAGTTATGTACCGCATGCAAAATGGTTTTGAACATAATCAAGTTTCTATATGTGTTGTCGTTCAAAAAATGGTTTTTCCGCAGGCTTCAGGAATTTTATTTACCGCTGATCCGATTACTTCTAACCGGAAGGTGTTATCAATCGATGCCAGTTTTGGACTTGGCGAGGCATTAGTATCGGGACTCGTCTCTGCCGATAATTATAAAGTAAAAGAAGGCGAAATCATCGATAAGATGATTGCAACTAAAAAATTAGCTATTTATGCTTTAAAAGAGGGCGGAACTGAGACGAAACAGAATGATCCGGTTCAGCAAAAAATTCAAACACTATCTGACCAACAAATATTACAACTAGTACAGATTGGAAGGCAGATTGAAGCTTATTTTGGCTGTCCGCAAGATATTGAATGGTGTTTAGTCGATCACACATTTTATATTGTCCAAAGTCGTCCGATAACGACTTTATATCCAATTCCGGAAGAAAATGATGAGGGAAATCATGTGTATATATCAGTTGGTCATCAACAAATGATGACCGATGCGATGAAACCACTAGGGTTATCTTTTTTCTTGTTAACGACTAATGCTCCTATGCGTAAAGCGGGAGGCAGGCTGTTTGTAGATGCTACGCAACAATTAGTTTTACCTGCTAGCAGAGATTATTTAATAAACACTTTAGGGAAATCGGATCCACTTGTAAGAGATGCATTGACGACTATAATCGAGCGAGATGATTTTATTACATTATTACCGGATGAAGAAAAAGAAAAGAGTGCTAGCAAAAGTAAGCCACCAGTAAGTTCGCAACCAGAAATCGAAAATGATCCAGCAATCGTTACGGAATTAATAAGGAATAGTGAAGCATCACTTGAAGAGTTAAAACAAAATATGCAACTGAAATCAGGAGTGGATGTATTTGATTTTATTTTGGAAGACATCCAGCAATTAAAGAAAGTATTATTTAATCCGCAAAGTATAGCTGTCATCATGGCAGGTATGAATGCTTCAACATGGATTAATGAAAAAATGGAGCAGTGGCTAGGTGAAAAGAATGCAGCAGACATGCTTTCTCAATCGGTACAAAATAATATTACATCAGAAATGGGTTTAGCATTAATGGATGTTGCTGATGCAATTCGTCCGTATCCAGAAGTTATTACGTATTTACAACATGTAGAAGATGATAGCTTTTTAGATGAACTTGTTCAGTATAAAGGCGGAGAAAAAGCTCGAGATACAATCGTTGCTTTTCTAAATAAATACGGAATGAGATGTAGCGGCGAAATCGACATTACGAAAACGCGCTGGAGTGAGAAGCCAACAACCATTATCCCAATGATATTAAATAATATAAGAGACTTTGAAGCGGGTGCTAGTAAGCAGAAGTTTGAAAAAGGACTGCAGGAAGCTTTGAATAAAGAAGAAGAGTTATTAGACCGATTGCAACATTTGCCGAAGGGTAAACAAAAAGTAGAAGAAACGAAGCGAATGATTCGTAATATCCGTAATTTTATTGGCTATCGTGAATATCCAAAATACGGCATGATTAATCGTTACTTCATATATAAGCAGGCGTTATTGAAAGAAGCTGAGCAACTTGTACAAAGCGGTGTCATTTGTGAGGTCGATGATATATACTATCTAACTTTTGAAGAACTTCATGAAGTTGTTCGCACGAATAAACTAGATTACGAGCTTATCCAAAAACAAAAAAATGATTATAAATTGTATGAAAAACTAACGCCTCCCCGCGTTATGACGTCTGATGGAGAAATCATTACAGGTAAATATAAACGAGAAAATCTCCCAGCTGACGCGATTGTAGGCCTGCCTGTTTCTTCCGGAGTAGTTGAGGGGAGAGCCCGTGTTATTTTAAACATGGAAGAAGCGAATTTAGAAGAGGGAGATATATTAGTTACTGCTTTTACTGATCCTGGCTGGACACCGTTGTTTGTTTCTATAAAAGGGTTAGTGACCGAAGTTGGTGGGCTCATGACACACGGGGCAGTGATTGCACGTGAATATGGATTACCAGCAGTTGTTGGAGTAGAGAATGCTACGAAACGAATCAAAGATGGGCAACGAATTCGGGTACATGGAACAGAAGGGTATATTGAAATGTTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,859 total letters Query= IDP02374.103 Length=3009 Score E Sequences producing significant alignments: (Bits) Value wt 4697 0.0 12E_E6_IDP02374.103_T7term.seq 1270 0.0 06E_E6_IDP02374.103_T7short.seq 616 4e-179 M S S F V L D F Q E I E K T Q L S L V G G K G L N L G A L Query_1 202 ATGAGTTCTTTCGTTCTCGATTTTCAGGAAATAGAAAAAACGCAGCTTTCACTCGTTGGTG-GAAAAGGTTTGAATTTAGGGGCGTTATC 290 2 1 .............................................................-............................ 89 0 66 ................A-..NG.GA..................................NCC.....N.N.................... 154 S N M Q G I Q V P E G F C V T T A G Y E K A I E Q N E E L Q Query_1 291 CAACATGCAAGGGATACAAGTGCCAGAAGGTTTTTGTGTTACAACGGCAGGATATGAAAAGGCCATCGAGCAAAATGAGGAGCTTCAAGC 380 2 90 .......................................................................................... 179 0 155 ......................N................................................................... 244 A L L Q Q L T K L K R E E R V R I G E I S K K I R E N I M A Query_1 381 TTTGTTGCAACAGCTAACAAAGTTGAAACGTGAAGAGCGAGTTCGAATTGGTGAAATCAGCAAGAAGATTAGAGAAAACATTATGGCGGT 470 2 180 .......................................................................................... 269 0 245 ...........................................................N.............................. 334 V Q I P S D V V E A V A H Y L S R F G N E H A Y A V R S S A Query_1 471 ACAAATCCCTTCTGATGTAGTGGAAGCAGTAGCTCATTATCTCTCACGCTTTGGAAATGAACATGCGTATGCCGTACGCTCTAGTGCGAC 560 2 270 .......................................................................................... 359 0 335 ............................................N......................................N...... 424 T A E D L P Y A S F A G Q Q D T Y L N I I G E N A I L Q H V Query_1 561 GGCTGAAGATTTACCGTATGCCTCGTTTGCTGGTCAACAAGATACGTATTTAAATATTATCGGAGAAAACGCAATTTTACAGCATGTAAG 650 2 360 .......................................................................................... 449 0 425 ................... 443 R K C W A S L F T D R A V M Y R M Q N G F E H N Q V S I C V Query_1 651 AAAGTGCTGGGCTTCTTTATTTACAGATCGTGCAGTTATGTACCGCATGCAAAATGGTTTTGAACATAATCAAGTTTCTATATGTGTTGT 740 2 450 .......................................................................................... 539 1 776 ........... 786 V V Q K M V F P Q A S G I L F T A D P I T S N R K V L S I D Query_1 741 CGTTCAAAAAATGGTTTTTCCGCAGGCTTCAGGAATTTTATTTACCGCTGATCCGATTACTTCTAACCGGAAGGTGTTATCAATCGATGC 830 2 540 .......................................................................................... 629 A S F G L G E A L V S G L V S A D N Y K V K E G E I I D K M Query_1 831 CAGTTTTGGACTTGGCGAGGCATTAGTATCGGGACTCGTCTCTGCCGATAATTATAAAGTAAAAGAAGGCGAAATCATCGATAAGATGAT 920 2 630 .......................................................................................... 719 I A T K K L A I Y A L K E G G T E T K Q N D P V Q Q K I Q T Query_1 921 TGCAACTAAAAAATTAGCTATTTATGCTTTAAAAGAGGGCGGAACTGAGACGAAACAGAATGATCCGGTTCAGCAAAAAATTCAAACACT 1010 2 720 .......................................................................................... 809 L S D Q Q I L Q L V Q I G R Q I E A Y F G C P Q D I E W C L Query_1 1011 ATCTGACCAACAAATATTACAACTAGTACAGATTGGAAGGCAGATTGAAGCTTATTTTGGCTGTCCGCAAGATATTGAATGGTGTTTAGT 1100 2 810 .......................................................................................... 899 V D H T F Y I V Q S R P I T T L Y P I P E E N D E G N H V Y Query_1 1101 CGATCACACATTTTATATTGTCCAAAGTCGTCCGATAACGACTTTATATCCAATTCCGGAAGAAAATGATGAGGGAAATCATGTGTATAT 1190 2 900 .......................................................................................... 989 2 2325 ........... 2315 1 349 ........... 359 I S V G H Q Q M M T D A M K P L G L S F F L L T T N A P M R Query_1 1191 ATCAGTTGGTCATCAACAAATGATGACCGATGCGATGAAACCACTAGGGTTATCTTTTTTCTTGTTAACGACTAATGCTCCTATGCGTAA 1280 2 990 .......................................................................................... 1079 K A G G R L F V D A T Q Q L V L P A S R D Y L I N T L G K S Query_1 1281 AGCGGGAGGCAGGCTGTTTGTAGATGCTACGCAACAATTAGTTTTACCTGCTAGCAGAGATTATTTAATAAACACTTTAGGGAAATCGGA 1370 2 1080 .......................................................................................... 1169 2 1830 ........... 1820 D P L V R D A L T T I I E R D D F I T L L P D E E K E K S A Query_1 1371 TCCACTTGTAAGAGATGCATTGACGACTATAATCGAGCGAGATGATTTTATTACATTATTACCGGATGAAGAAAAAGAAAAGAGTGCTAG 1460 2 1170 .......................................................................................... 1259 S K S K P P V S S Q P E I E N D P A I V T E L I R N S E A S Query_1 1461 CAAAAGTAAGCCACCAGTAAGTTCGCAACCAGAAATCGAAAATGATCCAGCAATCGTTACGGAATTAATAAGGAATAGTGAAGCATCACT 1550 2 1260 .......................................................................................... 1349 L E E L K Q N M Q L K S G V D V F D F I L E D I Q Q L K K V Query_1 1551 TGAAGAGTTAAAACAAAATATGCAACTGAAATCAGGAGTGGATGTATTTGATTTTATTTTGGAAGACATCCAGCAATTAAAGAAAGTATT 1640 2 1350 .......................................................................................... 1439 2 1831 .... 1828 0 44 ........... 34 L F N P Q S I A V I M A G M N A S T W I N E K M E Q W L G E Query_1 1641 ATTTAATCCGCAAAGTATAGCTGTCATCATGGCAGGTATGAATGCTTCAACATGGATTAATGAAAAAATGGAGCAGTGGCTAGGTGAAAA 1730 2 1440 .......................................................................................... 1529 2 1827 ....... 1821 K N A A D M L S Q S V Q N N I T S E M G L A L M D V A D A I Query_1 1731 GAATGCAGCAGACATGCTTTCTCAATCGGTACAAAATAATATTACATCAGAAATGGGTTTAGCATTAATGGATGTTGCTGATGCAATTCG 1820 2 1530 .......................................................................................... 1619 R P Y P E V I T Y L Q H V E D D S F L D E L V Q Y K G G E K Query_1 1821 TCCGTATCCAGAAGTTATTACGTATTTACAACATGTAGAAGATGATAGCTTTTTAGATGAACTTGTTCAGTATAAAGGCGGAGAAAAAGC 1910 2 1620 .......................................................................................... 1709 A R D T I V A F L N K Y G M R C S G E I D I T K T R W S E K Query_1 1911 TCGAGATACAATCGTTGCTTTTCTAAATAAATACGGAATGAGATGTAGCGGCGAAATCGACATTACGAAAACGCGCTGGAGTGAGAAGCC 2000 2 1710 .......................................................................................... 1799 P T T I I P M I L N N I R D F E A G A S K Q K F E K G L Q E Query_1 2001 AACAACCATTATCCCAATGATATTAAATAATATAAGAGACTTTGAAGCGGGTGCTAGTAAGCAGAAGTTTGAAAAAGGACTGCAGGAAGC 2090 2 1800 .......................................................................................... 1889 2 1149 ........... 1139 2 1446 ........... 1436 1 810 ....N.............. 792 A L N K E E E L L D R L Q H L P K G K Q K V E E T K R M I Query_1 2091 TTTG--AATAAAGAAGAAGAGTTATTAGACCGATTGCAA-CATTTGCCGAAGGG-TAAACAAAAAGTAGAAGAAACGAAGCGA-ATGATT 2175 2 1890 ....--.................................-..............-............................-...... 1974 1 791 ...TGA.................................A.....N........G............................N...... 702 R N I R N F I G Y R E Y P K Y G M I N R Y F I Y K Q A L L Query_1 2176 -CGTAA-TATCCGTAATTTTATTGGCTATCGTGAATATCCAAAATACGGCATGATTAATCGTTACTTCATATATAAGCAGGCGTTATTGA 2263 2 1975 -.....-................................................................................... 2062 1 701 T.....A......................................................N............................ 612 K E A E Q L V Q S G V I C E V D D I Y Y L T F E E L H E V V Query_1 2264 AAGAAGCTGAGCAACTTGTACAAAGCGGTGTCATTTGTGAGGTCGATGATATATACTATCTAACTTTTGAAGAACTTCATGAAGTTGTTC 2353 2 2063 .......................................................................................... 2152 2 2152 ...C..............C... 2131 1 611 .......................................................................................... 522 1 522 ...C..............C... 543 R T N K L D Y E L I Q K Q K N D Y K L Y E K L T P P R V M T Query_1 2354 GCACGAATAAACTAGATTACGAGCTTATCCaaaaacaaaaaaaTGATTATAAATTGTATGAAAAACTAACGCCTCCCCGCGTTATGACGT 2443 2 2153 .......................................................................................... 2242 1 521 .......................................................................................... 432 S D G E I I T G K Y K R E N L P A D A I V G L P V S S G V V Query_1 2444 CTGATGGAGAAATCATTACAGGTAAATATAAACGAGAAAATCTCCCAGCTGACGCGATTGTAGGCCTGCCTGTTTCTTCCGGAGTAGTTG 2533 2 2243 .......................................................................................... 2332 2 964 ........... 954 1 431 .......................................................................................... 342 E G R A R V I L N M E E A N L E E G D I L V T A F T D P G W Query_1 2534 AGGGGAGAGCCCGTGTTATTTTAAACATGGAAGAAGCGAATTTAGAAGAGGGAGATATATTAGTTACTGCTTTTACTGATCCTGGCTGGA 2623 2 2333 .......................................................................................... 2422 1 341 .......................................................................................... 252 T P L F V S I K G L V T E V G G L M T H G A V I A R E Y G L Query_1 2624 CACCGTTGTTTGTTTCTATAAAAGGGTTAGTGACCGAAGTTGGTGGGCTCATGACACACGGGGCAGTGATTGCACGTGAATATGGATTAC 2713 2 2423 .......................................................................................... 2512 1 251 .......................................................................................... 162 P A V V G V E N A T K R I K D G Q R I R V H G T E G Y I E M Query_1 2714 CAGCAGTTGTTGGAGTAGAGAATGCTACGAAACGAATCAAAGATGGGCAACGAATTCGGGTACATGGAACAGAAGGGTATATTGAAATGT 2803 2 2513 .......................................................................................... 2602 1 161 .......................................................................................... 72 L * H W K W I T D P N S S S V D K L A A A L E H H H H H H * Query_1 2804 TGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAG 2893 2 2603 .. 2604 1 71 ..C.T... 64 1 70 ...T..T.....T.....T.A.. 48 D P A A N K A R K E A E L A A A T A E Q * L A * P L G A S K Query_1 2894 ATCCGGCTGCTAACAAAG-CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAA 2982 1 47 .......N..........C....... 22 R V L R G F L L Query_1 2983 CGGGTCTTGAGGGGTTTTTTGCTGAA 3008 2 799 ............ 788 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11393536 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 3,859 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP02490.102 (IDP02490.1-386.F..pMCSG68)
MNIHEYQGKAVLRSYGVSVPNGKVAFTVEEAVEAAKELGTDVCVVKAQIHAGGRGKAGGVKVAKNLDEVRTYAESILGTTLVTHQTGPEGKEVKRLLIEEGCDIKKEYYVGLVLDRATSQVVLMASEEGGTEIEEVAEKTPEKIFKEYIDPAVGLQGFQARRIAFNINIPKELVGQAVKFMMGLYRAFIEKDCSIAEINPLVTTGDGKVMALDAKLNFDSNALYRHKDILELRDLDEEDAKEIEASKYDLNYIPLDGNIGCMVNGAGLAMATMDIIKHYHGDPANFLDVGGGATAEKVTEAFKIILSDKNVKGIFVNIFGGIMKCDVIAEGVIEATKQVGLELPLVVRLEGTNVELGKKILNESGLNIVAAESMTDGAQKIVSLVG
ATGAATATCCATGAGTACCAAGGTAAGGCAGTCCTTAGAAGTTATGGGGTTAGCGTTCCGAACGGGAAGGTTGCATTTACAGTAGAAGAAGCTGTAGAAGCAGCGAAAGAATTAGGAACGGATGTATGTGTAGTAAAAGCACAAATTCACGCTGGTGGACGCGGCAAAGCTGGCGGAGTAAAAGTTGCAAAAAATTTAGATGAAGTTCGTACATATGCAGAGAGCATTTTAGGAACTACACTTGTGACACATCAAACAGGTCCTGAAGGTAAGGAAGTAAAACGCTTACTTATCGAAGAAGGTTGCGATATTAAAAAAGAATATTATGTAGGTCTTGTTTTAGATCGTGCAACTTCTCAAGTTGTTTTAATGGCGTCTGAAGAAGGCGGAACAGAAATTGAAGAAGTAGCAGAAAAAACACCAGAAAAAATCTTTAAAGAATATATTGATCCAGCAGTAGGTTTACAAGGCTTCCAAGCACGCCGGATTGCATTTAACATCAATATTCCAAAAGAGCTTGTAGGACAAGCTGTGAAGTTTATGATGGGCTTATACCGTGCGTTTATCGAAAAAGATTGTTCTATCGCAGAGATTAATCCACTTGTTACAACAGGTGACGGTAAAGTTATGGCATTAGATGCGAAATTAAACTTTGATTCTAACGCTTTATATCGCCATAAAGACATTTTAGAACTTCGTGATCTTGATGAAGAAGATGCAAAAGAAATCGAAGCTTCTAAATATGACTTAAACTACATTCCTTTAGATGGAAATATCGGTTGTATGGTTAATGGTGCAGGTTTAGCGATGGCTACGATGGATATCATTAAACATTACCATGGCGATCCAGCTAACTTCTTAGATGTTGGTGGCGGTGCGACAGCTGAAAAAGTTACAGAAGCATTTAAAATTATCCTTTCTGACAAAAATGTAAAAGGTATCTTCGTTAACATTTTTGGTGGCATTATGAAGTGTGATGTTATTGCAGAAGGTGTTATTGAAGCAACGAAACAAGTAGGTCTTGAATTGCCTTTAGTTGTACGTCTTGAAGGTACAAACGTTGAGTTAGGTAAGAAAATTTTAAATGAGTCTGGCTTAAATATTGTTGCAGCAGAATCTATGACAGACGGTGCACAAAAAATTGTTTCACTAGTGGGC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,540 total letters Query= IDP02490.102 Length=1563 Score E Sequences producing significant alignments: (Bits) Value wt 2089 0.0 11G_G5_IDP02490.102_T7term.seq 1638 0.0 05G_G5_IDP02490.102_T7short.seq 1442 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 58 TTCCCCTCTAGAAATAATTTTGTTTAACTTTAAG-AAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTG 146 0 16 ........CTAN......................T......G.-..G..... 66 W S H P Q F E K G T E N L Y F Q S N A M N I H E Y Q G K A V Query_1 147 GTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGAATATCCATGAGTACCAAGGTAAGGCAGTCCT 236 2 1 ................................... 35 0 64 ....................T.............. 98 L R S Y G V S V P N G K V A F T V E E A V E A A K E L G T D Query_1 237 TAGAAGTTATGGGGTTAGCGTTCCGAACGGGAAGGTTGCATTTACAGTAGAAGAAGCTGTAGAAGCAGCGAAAGAATTAGGAACGGATGT 326 2 36 .......................................................................................... 125 1 1106 .... 1103 0 99 ..N...C.................T....................C.N............T-..........N................. 187 V C V V K A Q I H A G G R G K A G G V K V A K N L D E V R Query_1 327 A-TGTGT-AGTAAAAG-CACAAATTCA-CGCTGGTGGACGCGGCAAAGCTGGCGGAGTAAAAGTTGCAAAAAATTTAGATGAAG-TTCGT 411 2 126 .-.....-........-..........-........................................................-..... 210 1 1102 .A.....T........G.T.C......N...CT.NT.N.NN..........CG...N....G--.N..........CA......G..... 1015 0 188 .-.....-........-..........-.T......................................................-..... 272 T Y A E S I L G T T L V T H Q T G P E G K E V K R L L I E Query_1 412 ACAT-ATGCAGAGAGCATTTTAGGAACTACACTTGTGACACATCAAACAGGTCCTGAAGGTAAGGAAGTAAAACGCTTACTTATCGAAGA 500 2 211 ....-..................................................................................... 299 1 1014 ....N.G..........................-.....-.....................N...G......-................. 928 0 273 ..T.-.....T.....................................NT.C......N...............C..........N.... 361 E G C D I K K E Y Y V G L V L D R A T S Q V V L M A S E E G Query_1 501 AGGTTGCGATATTAAAAAAGAATATTATGTAGGTCTTGTTTTAGATCGTGCAACTTCTCAAGTTGTTTTAATGGCGTCTGAAGAAGGCGG 590 2 300 .......................................................................................... 389 1 927 .....N.........................N.......................................................... 838 1 517 ........AT.C 506 0 362 .C.....N.......................N......N.............N............N........................ 451 G T E I E E V A E K T P E K I F K E Y I D P A V G L Q G F Query_1 591 AACAGAAATTGAAGAAGTAGCAGAAAAAACACCAGAAAAA-ATCTTTAAAGAATATA-TTGATCCAGCAGTAGGTTTACAAGGCTT-CCA 677 2 390 ........................................-................-............................-... 476 1 837 ............................-...........N................N........................N...T... 749 1 505 ..A......N.... 492 0 452 .............N..T..N.......N............-................-...........N....N...........-... 538 Q A R R I A F N I N I P K E L V G Q A V K F M M G L Y R A F Query_1 678 AGCACGCCGGATTGCATTTAACATCAATATTCCAAAAGAGCTTGTAGGACAAGCTGTGAAGTTTATGATGGGCTTATACCGTGCGTTTAT 767 2 477 .......................................................................................... 566 1 748 .........................N................................................................ 659 0 539 .....................................N.................................................... 628 I E K D C S I A E I N P L V T T G D G K V M A L D A K L N F Query_1 768 CGAAAAAGATTGTTCTATCGCAGAGATTAATCCACTTGTTACAACAGGTGACGGTAAAGTTATGGCATTAGATGCGAAATTAAACTTTGA 857 2 567 .......................................................................................... 656 1 658 .......................................................................................... 569 0 629 .............................................NN.........N..NN.........N....N.............. 718 D S N A L Y R H K D I L E L R D L D E E D A K E I E A S K Y Query_1 858 TTCTAACGCTTTATAT-CGCCATAAAGACATTTTAGAACTTCGTGATCTTGATGAAGAAGATGCAAAAGAAATCGAAGCTTCTAAATATG 946 2 657 ................-......................................................................... 745 1 568 ................-..............N.........................................N................ 480 0 719 ................N....N...............N..................T....N..NNT.N..................... 808 D L N Y I P L D G N I G C M V N G A G L A M A T M D I I K H Query_1 947 ACTTAAACTACATTCCTTTAGATGGAAATATCGGTTGTATGGTTAATGGTGCAGGTTTAG-CGATGGCTACGATGGATATCATTAAACAT 1035 2 746 ............................................................-............................. 834 1 479 ............................................................-...N......................... 391 0 809 ............................N........N...........C..C.......C..........N..............N.N. 898 Y H G D P A N F L D V G G G A T A E K V T E A F K I I L S Query_1 1036 TACCATGGCGATCCAGCTAACTTCTTAGATGTTGG-TGGCGGTGCGACAGCTGAAAAAGTTACAGAAGCATTTAAAATTATCCTTTCTGA 1124 2 835 ...................................-...................................................... 923 2 1093 ....CT...........T.. 1074 1 390 .G.................................-...................................................... 302 1 132 ....CT.....N.....T.. 151 0 899 ...............T.NT........N.......N...............N.....GN.AC.T.........T.N..CN.....C.... 988 D K N V K G I F V N I F G G I M K C D V I A E G V I E A T Query_1 1125 -CAAAAATGTAAAAGGTATCTTCGTTAACA-TTTTTGGTGG-CATTATGAAGTGT-GATGTTATTGCAGAAGGTGTT-ATTGAAGCAACG 1209 2 924 -.............................-..........-.............-.....................-............ 1008 1 301 -.............................-..........-............N-.....................-............ 217 0 989 C..N.N.......C.......G....N...C.N........C...AT.....N..C....C.TA...N..T.T....A......N.-..T 1077 K Q V G L E L P L V V R L E G T N V E L G K K I L N E S Query_1 1210 AAACAAGTAGGT-CTTGAATT---GCCTTTAGTTGTACGTC--TTGAAGGTACAAACGTTGAGTTAGGTAAGAAAATTTTAAATGAGTCT 1293 2 1009 ............-........---.................--............................................... 1092 2 915 ..T...........CT... 897 1 216 ............-.N......---.................--....................................N.......... 133 1 310 ..T...........CT... 328 0 1078 ....C.......C......N.TNGC.......C...NT...CT.G.....NN.C... 1134 G L N I V A A E S M T D G A Q K I V S L V G * H W K W I T Query_1 1294 GGCTTAAATATTGTTGCAGCAGAATCTATGACAGACGGTGCACAAAAAATTGTTTC-ACTAGTGGGCTAACATTGGAAGTGGATAACGGA 1382 2 1093 ........................................................-.......... 1158 2 896 . 896 1 132 ..N.....................................................G.N........C.T... 60 1 329 . 329 D P N S S S V D K L A A A L E H H H H H H * D P A A N K A R Query_1 1383 TCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAA 1472 1 65 ..T..T.....T.....T.A......................A.... 19 K E Query_1 1473 GGAA 1476 1 18 A... 15 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5402124 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 3,540 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP04614.102 (IDP04614.1-366.F..pMCSG68)
MITLQRTPLFDVYAKYGGKTIDFGGWELPVQFSSIKEEHEAVRTAAGLFDVSHMGEVEVKGVDSLAFLQRVVTNDVSTLKVGGAQYTAMCYENGGTVDDLLIYKRGEEDYLLVINASNIEKDYEWLASHVIGDATVVNVSSEVAQLAIQGPKAEGILQKVVSEDLKEIKFFKFKNDILVDGIPALVSRTGYTGEDGFEIYCKSEDAAKLWEKLLEVGAEEGLKACGLGARDTLRFEATLPLYGQELSKDITPIEAGIGFAVKTNKEADFFGKATLKEQKENGAPRKLVGIEVIERGIPRTHYPVFIGEEKIGEVTSGTQSPTLKKSIGLALIDVKYAAVDTEVEIEIRNKRVKAVVVPTPFYKRSK
ATGATTACATTACAACGTACACCGTTATTTGATGTATACGCGAAGTATGGTGGGAAAACAATCGACTTCGGTGGTTGGGAGTTACCAGTTCAATTTTCAAGCATTAAAGAAGAACATGAAGCTGTACGTACAGCGGCGGGTTTGTTCGATGTGTCTCATATGGGAGAAGTTGAGGTAAAAGGTGTAGATAGTTTAGCATTTTTACAACGTGTTGTTACAAATGACGTATCTACCTTAAAGGTAGGAGGCGCACAATATACAGCAATGTGCTACGAAAATGGTGGTACAGTAGATGATTTATTAATCTACAAACGTGGTGAAGAAGACTATTTATTAGTTATCAACGCATCAAATATCGAGAAAGATTACGAATGGTTAGCTAGCCATGTAATTGGCGATGCGACAGTAGTCAATGTTTCTAGCGAAGTTGCACAGCTTGCAATTCAAGGACCAAAAGCAGAAGGCATTTTACAAAAAGTTGTGTCAGAAGATTTAAAAGAAATTAAGTTCTTTAAATTTAAAAACGATATTCTTGTAGATGGAATTCCAGCACTTGTATCTCGTACAGGTTACACAGGTGAAGATGGATTTGAAATTTACTGTAAGAGTGAAGATGCAGCAAAACTTTGGGAGAAACTTCTTGAAGTTGGAGCAGAAGAAGGCTTAAAAGCATGTGGTTTAGGTGCTCGTGATACACTTCGCTTCGAAGCAACATTACCACTTTATGGTCAAGAATTATCAAAAGATATTACACCGATTGAAGCTGGAATTGGCTTTGCGGTAAAAACAAATAAAGAAGCAGACTTCTTTGGAAAAGCAACGTTAAAAGAGCAAAAAGAAAACGGTGCGCCTCGCAAATTAGTCGGCATCGAAGTAATTGAACGTGGAATTCCTCGTACGCATTACCCTGTATTTATTGGAGAAGAAAAGATCGGGGAAGTAACAAGTGGTACACAATCTCCAACGTTAAAGAAAAGCATTGGTTTAGCACTAATTGATGTAAAATACGCAGCAGTTGATACAGAAGTAGAAATTGAAATTCGTAATAAACGCGTCAAAGCAGTAGTTGTTCCAACACCATTTTATAAACGTTCAAAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,586 total letters Query= IDP04614.102 Length=1503 Score E Sequences producing significant alignments: (Bits) Value wt 1981 0.0 11H_H5_IDP04614.102_T7term.seq 1813 0.0 05H_H5_IDP04614.102_T7short.seq 1786 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 60 CCCCTC-TAGAAATAATTTTGTTTAACTTTAAG-AAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGG 147 0 21 ......C..N.......................T......G.-..G..... 70 S H P Q F E K G T E N L Y F Q S N A M I T L Q R T P L F D V Query_1 148 TCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGATTACATTACAACGTACACCGTTATTTGATGTA 237 2 1 .................................... 36 1 1132 .......N 1125 0 68 .................................... 103 Y A K Y G G K T I D F G G W E L P V Q F S S I K E E H E A V Query_1 238 TACGCGAAGTATGGTGGGAAAACAATCGACTTCGGTGGTTGGGAGTTACCAGTTCAATTTTCAAGCATTAAAGAAGAACATGAAGCTGTA 327 2 37 .......................................................................................... 126 2 134 ...... 129 1 1124 NT..N...............NN--....NN.....N.-.........NN-....N.-..............-.........N........ 1041 1 1036 ... 1038 0 104 .......................................................................................... 193 0 201 ...... 196 R T A A G L F D V S H M G E V E V K G V D S L A F L Q R V Query_1 328 CGTACA-GCGGCGGGTTTGTT-CGATGT-GTCTCATATGGGAGAAGTTGAGGTAAAAGGTGTAGATAGTTTAGCATTTTTACAACGTGTT 414 2 127 ......-..............-......-............................................................. 213 2 128 ......-.. 121 1 1040 .....NN......N.......T......N............................................................. 951 1 1039 ......-.. 1046 0 194 ......-..............-......-............................................................. 280 0 195 ......-.. 188 V T N D V S T L K V G G A Q Y T A M C Y E N G G T V D D L L Query_1 415 GTTACAAATGACGTATCTACCTTAAAGGTAGGAGGCGCACAATATACAGCAATGTGCTACGAAAATGGTGGTACAGTAGATGATTTATTA 504 2 214 .......................................................................................... 303 1 950 .......................................................................................... 861 0 281 .......................................................................................... 370 I Y K R G E E D Y L L V I N A S N I E K D Y E W L A S H V I Query_1 505 ATCTACAAACGTGGTGAAGAAGACTATTTATTAGTTATCAACGCATCAAATATCGAGAAAGATTACGAATGGTTAGCTAGCCATGTAATT 594 2 304 .......................................................................................... 393 1 860 .......................................................................................... 771 0 371 .......................................................................................... 460 G D A T V V N V S S E V A Q L A I Q G P K A E G I L Q K V V Query_1 595 GGCGATGCGACAGTAGTCAATGTTTCTAGCGAAGTTGCACAGCTTGCAATTCAAGGACCAAAAGCAGAAGGCATTTTACAAAAAGTTGTG 684 2 394 .......................................................................................... 483 1 770 .......................................................................................... 681 0 461 .......................................................................................... 550 S E D L K E I K F F K F K N D I L V D G I P A L V S R T G Y Query_1 685 TCAGAAGATTTAAAAGAAATTAAGTTCTTTAAATTTAAAAACGATATTCTTGTAGATGGAATTCCAGCACTTGTATCTCGTACAGGTTAC 774 2 484 .......................................................................................... 573 2 522 ............ 511 1 680 .......................................................................................... 591 1 642 ............ 653 0 551 .......................................................................................... 640 0 589 ............ 578 T G E D G F E I Y C K S E D A A K L W E K L L E V G A E E G Query_1 775 ACAGGTGAAGATGGATTTGAAATTTACTGTAAGAGTGAAGATGCAGCAAAACTTTGGGAGAAACTTCTTGAAGTTGGAGCAGAAGAAGGC 864 2 574 .......................................................................................... 663 1 590 .......................................................................................... 501 0 641 .......................................................................................... 730 L K A C G L G A R D T L R F E A T L P L Y G Q E L S K D I Query_1 865 TTAAAAGCATGTGGTTTAGGTGCTCGTGATACACTTCGCTTCGAAGCAACATT--ACCAC-TTTATGGTCAAGAATTATCAAAAGATATT 951 2 664 .....................................................--.....-............................. 750 1 500 .....................................................--.....-............................. 414 0 731 .....................................................TAC....T............................. 820 T P I E A G I G F A V K T N K E A D F F G K A T L K E Q K Query_1 952 ACACCGATTGAAGCTGGAA-TTGGCTTTGCGGTaaaaacaaataaagaagcagacttctttggaaaagcaacgttaaaagagcaaaaaga 1040 2 751 ...................-...................................................................... 839 1 413 ...................-...................................................................... 325 0 821 ...................N.............-........................................................ 909 E N G A P R K L V G I E V I E R G I P R T H Y P V F I G E E Query_1 1041 aaaCGGTGCGCCTCGCAAATTAGTCGGCATCGAAGTAATTGAACGTGGAA-TTCCTCGTACGCATTACCCTGTATTTATTGGAGAAGAAA 1129 2 840 ..................................................-....................................... 928 1 324 ..................................................-....................................... 236 0 910 ..................................................N....................................... 999 K I G E V T S G T Q S P T L K K S I G L A L I D V K Y A Query_1 1130 AGATCGGGG-AAGTAACAAG-TGGTACACAATC-TCCAACG-TTAAAGAAAAGCATTGG-TTTAG-CACTAATTGATGTAAAA-TACGCA 1212 2 929 .........-..........-............-.......-.................-.....-.................-...... 1011 1 235 .........-..........-............-.......-.................-.....-.................-...... 153 0 1000 .........T..........N..T......T..N.......T.................C.....C.................T...... 1089 A V D T E V E I E I R N K R V K A V V V P T P F Y K R S Query_1 1213 GCAGTTGATACAGAAGTAGAAATT-GAAA-TTCGTAA-TAAACGCG-TCAAA-GCAGTAGTTGTTCCAACACCATTTTATAAACGTTCAA 1297 2 1012 ........................-....-.......-........-.....-..................................... 1096 1 152 ........................-....-.......-........-.....-..................................... 68 0 1090 .............-.N........N....T.......N...C....N.....G....N....-N.N....... 1160 K * H W K W I T D P N S S S V D K L A A A L E H H H H H H * Query_1 1298 AGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAG 1387 2 1097 .. 1098 1 67 ..C.T... 60 1 67 ....T..T.....T.....T.A.. 44 D P A A N K A R K E A E Query_1 1388 ATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTT 1424 1 43 ..........................A...GNN.... 7 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5261006 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 3,586 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP05031.102 (IDP05031.22-528.P(528)..pMCSG68)
SGEKDSKQAGTSKSGIPKDGGTLTIGVSDNPETMNPLYANDRVSLTVQQALYAPLYHMEDGKKKFVLAESFTPSEDQLTWTLKLKDNLKWHDGKKITSDDIAFTFQSILDEKQNSSSRENFVFKGKPLEVKKVDELTTQFVLPQVSASFEGVMNDFFPIPKHVFEGEADLAKSKKNLQPVGSGPFKFKEYKSDEYVALDRFDDYVGGKAKLDSIVYRVVKDRNTANVSLQNGQINMKMIEPQDFKKLDSTGNFSMVTFPEGRLFYLSYNMNTDLMKKKEVRQAIAHALDKKEMINSAFVSSQFAEPANSILTPDAMYYAKDIKEYKYDKKEAKDLLAKAGVKDKEKVRVMYVTNNKIMESLALYTQQKLQEVGLEVELNALDASAASDKSLDKENKEYDITFGGYIMGPEPDSYKSLFLSNAEYNYARYKNADFDKLWEEAAVETDKTKRAELYHKIQETAREDLPYLPIAYPKAVIAVDKKFDGLKEAKAIPVTMFEDVSKIYEVK
TCAGGGGAAAAAGATTCGAAGCAAGCAGGAACTAGTAAATCAGGGATTCCGAAAGATGGAGGAACGTTAACGATTGGTGTTAGCGATAACCCAGAGACGATGAATCCGCTTTATGCGAATGATCGTGTTTCGTTAACGGTGCAGCAAGCTTTATATGCACCGCTGTATCATATGGAAGACGGTAAGAAGAAGTTTGTTCTTGCTGAGAGCTTTACGCCTTCAGAGGATCAATTAACATGGACTTTAAAACTAAAGGATAATTTGAAATGGCATGACGGTAAGAAGATTACATCAGACGACATAGCATTTACATTCCAATCTATTTTAGATGAGAAGCAAAATAGCTCAAGTCGTGAAAACTTTGTTTTTAAAGGAAAACCGCTTGAGGTGAAAAAGGTAGATGAGTTAACAACTCAATTCGTTTTACCACAAGTAAGTGCATCTTTCGAAGGTGTGATGAATGATTTCTTCCCAATTCCGAAACATGTATTTGAAGGGGAAGCAGATTTAGCGAAGAGTAAGAAAAACTTACAGCCTGTAGGATCAGGTCCGTTTAAGTTTAAAGAGTATAAGTCTGATGAGTACGTTGCATTAGATCGATTTGATGATTATGTAGGTGGTAAAGCTAAATTAGATTCTATCGTATACCGCGTTGTAAAAGATCGTAATACAGCAAATGTTTCACTACAAAATGGTCAAATCAACATGAAGATGATTGAGCCACAAGACTTTAAGAAATTAGATAGTACAGGGAACTTCTCAATGGTGACATTCCCAGAAGGTAGATTATTCTACTTATCTTATAACATGAATACAGATCTGATGAAGAAAAAAGAAGTGCGCCAAGCAATTGCACATGCGTTAGATAAAAAAGAAATGATTAACTCAGCATTCGTTTCTAGTCAATTTGCAGAACCAGCAAATTCAATTTTAACGCCAGACGCTATGTATTATGCAAAAGACATTAAAGAGTATAAGTATGATAAAAAAGAAGCGAAAGATTTATTAGCGAAAGCTGGCGTGAAGGATAAAGAAAAAGTACGCGTTATGTATGTAACGAATAATAAAATTATGGAAAGCTTAGCGCTTTATACACAACAAAAATTACAAGAAGTTGGCTTAGAAGTTGAACTGAATGCATTAGATGCTAGTGCAGCAAGTGACAAAAGCTTAGATAAAGAGAATAAAGAATATGACATTACATTCGGTGGTTACATAATGGGACCTGAGCCAGATTCATATAAGAGCTTATTCTTAAGTAATGCTGAATACAACTATGCACGTTATAAAAACGCTGATTTCGATAAGTTATGGGAAGAAGCTGCAGTTGAAACAGATAAAACAAAACGCGCAGAACTATATCATAAAATTCAAGAGACAGCTAGAGAAGACTTACCTTATCTACCAATTGCGTATCCGAAAGCAGTTATTGCAGTAGATAAAAAGTTTGATGGATTGAAAGAAGCAAAAGCAATTCCTGTCACAATGTTTGAAGATGTATCTAAGATTTATGAAGTGAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,922 total letters Query= IDP05031.102 Length=1926 Score E Sequences producing significant alignments: (Bits) Value wt 2744 0.0 07H_H1_IDP05031.102_T7term.seq 1941 0.0 01H_H1_IDP05031.102_T7short.seq 1797 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 57 ATTCCCCT-CT-AGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTG 144 0 16 ........T..N......N....................................................................... 105 W S H P Q F E K G T E N L Y F Q S N A S G E K D S K Q A G T Query_1 145 TGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCTCAGGGGAAAAAGATTCGAAGCAAGCAGGAACT 234 2 1 ................................. 33 0 106 .......................................................................................... 195 S K S G I P K D G G T L T I G V S D N P E T M N P L Y A N D Query_1 235 AGTAAATCAGGGATTCCGAAAGATGGAGGAACGTTAACGATTGGTGTTAGCGATAACCCAGAGACGATGAATCCGCTTTATGCGAATGAT 324 2 34 .......................................................................................... 123 0 196 .......................................................................................... 285 R V S L T V Q Q A L Y A P L Y H M E D G K K K F V L A E S F Query_1 325 CGTGTTTCGTTAACGGTGCAGCAAGCTTTATATGCACCGCTGTATCATATGGAAGACGGTAAGAAGAAGTTTGTTCTTGCTGAGAGCTTT 414 2 124 .......................................................................................... 213 2 274 ............. 286 0 286 .......................................................................................... 375 0 436 ............. 448 T P S E D Q L T W T L K L K D N L K W H D G K K I T S D D I Query_1 415 ACGCCTTCAGAGGATCAATTAACATGGACTTTAAAACTAAAGGATAATTTGAAATGGCATGACGGTAAGAAGATTACATCAGACGACATA 504 2 214 .......................................................................................... 303 2 178 ............. 190 0 376 .......................................................................................... 465 0 340 ............. 352 A F T F Q S I L D E K Q N S S S R E N F V F K G K P L E V K Query_1 505 GCATTTACATTCCAATCTATTTTAGATGAGAAGCAAAATAGCTCAAGTCGTGAAAACTTTGTTTTTAAAGGAAAACCGCTTGAGGTGAAA 594 2 304 .......................................................................................... 393 0 466 .......................................................................................... 555 K V D E L T T Q F V L P Q V S A S F E G V M N D F F P I P Query_1 595 AAGGTAGATGAGTTAACAACTCAATTCGTTTTACCACAAGTAAGTGCATCTTTCGAAGGTGTGATGAATGATTTCTT-CCCAATTCCGAA 683 2 394 .............................................................................-............ 482 1 1200 .....N.NG.N.......... 1180 0 556 .............................................................................-............ 644 K H V F E G E A D L A K S K K N L Q P V G S G P F K F K E Query_1 684 ACATGTATTTGAAGGGGAAGCAGATTTAGCGAAG-AGTAAG-AAAAACTTACAGCCTGTAGGAT-CAGGTCC-GTTTAAG-TTTAAAGAG 768 2 483 ..................................-......-......................-.......-.......-......... 567 2 965 ........ 972 1 1179 ..NN.........A............-..N....G......G.......NN.............T.......C...N...-..-...... 1093 1 690 ........ 683 0 645 ..................................-......-......................-.......-.......T......... 730 Y K S D E Y V A L D R F D D Y V G G K A K L D S I V Y R V Query_1 769 TATAAG--TCTGATGAGTACGTTGCATTAGATCGATTTGATGATTATGTAGGTGGTAAAG-CTAAATTAGATTCTATCGTATACCGCGTT 855 2 568 ......--....................................................-............................. 654 2 973 ......--.A.... 984 1 1092 .....AGT.N......N.-..N......................................N.....................N....... 1004 1 682 ......--.A.... 671 0 731 ......--....................................................-............................. 817 V K D R N T A N V S L Q N G Q I N M K M I E P Q D F K K L Query_1 856 GTAAAAGATCGTAATAC-AGCAAATGTTTCACTACAAAA-TGGTCAAATCAACATGAAGATGATTGAGCCACAA-GACTTTAAGAAATTA 942 2 655 .................-.....................-..................................-............... 741 1 1003 ...............N.C.....................A.......N...-......................A............... 915 0 818 .-N..............-............N........-.....N............................-............... 903 D S T G N F S M V T F P E G R L F Y L S Y N M N T D L M K Query_1 943 GATAGTACAGGGAACTTCTCAATGGTGACATTCCCAGAAGGTAGATTA-TTCTACTTATCTTATAACATGAATACAGA-TCTGATGAAGA 1030 2 742 ................................................-.............................-........... 829 1 914 ................................................-.............................-........... 827 0 904 .............................N..................T........................NNC..NN........-N 992 K K E V R Q A I A H A L D K K E M I N S A F V S S Q F A E Query_1 1031 AAAAAGAAGTGCGCCAAGCAATTGCACAT-GCGTTAGATAAAAAAGAAATGATTAAC-TCAGCATTCGTTTCTAGTC-AATTTGCAGAA- 1116 2 830 .............................-...........................-...................-...........- 915 2 982 ............ 993 1 826 .............................-...........................-...................-...........- 741 1 673 ............ 662 0 993 .............................G.........-..............T..T..N......CN....NN..T.......C...C 1081 P A N S I L T P D A M Y Y A K D I K E Y K Y D K K E A K Query_1 1117 CCAGCAAA-TTCAATTTTAAC-GCCAGACG-CTATG-TATTATG--CAAAAGACATTAAAGAGTATAAGTATGATAAAAAAGAAGCGAAA 1200 2 916 ........-............-........-.....-.......--............................................ 999 2 560 ...............C.... 579 2 865 ............ 876 1 740 ........A............-........-.....-.......--............................................ 656 1 1100 ............. 1088 1 791 ............ 780 0 1082 .....N..T.........G..N...G....C..T..N......NGC....... 1134 0 723 ...............C.... 742 D L L A K A G V K D K E K V R V M Y V T N N K I M E S L A L Query_1 1201 GATTTATTAGCGAAAGCTGGCGTGAAGGATAAAGAAAAAGTACGCGTTATGTATGTAACGAATAATAAAATTATGGAAAGCTTAGCGCTT 1290 2 1000 .......................................................................................... 1089 1 655 .......................................................................................... 566 Y T Q Q K L Q E V G L E V E L N A L D A S A A S D K S L D K Query_1 1291 TATACACAACAAAAATTACAAGAAGTTGGCTTAGAAGTTGAACTGAATGCATTAGATGCTAGTGCAGCAAGTGACAAAAGCTTAGATAAA 1380 2 1090 .......................................................................................... 1179 1 565 .......................................................................................... 476 E N K E Y D I T F G G Y I M G P E P D S Y K S L F L S N A E Query_1 1381 GAGAATAAAGAATATGACATTACATTCGGTGGTTACATAATGGGACCTGAGCCAGATTCATATAAGAGCTTATTCTTAAGTAATGCTGAA 1470 2 1180 .......................................................................................... 1269 1 475 .......................................................................................... 386 Y N Y A R Y K N A D F D K L W E E A A V E T D K T K R A E L Query_1 1471 TACAACTATGCACGTTATAAAAACGCTGATTTCGATAAGTTATGGGAAGAAGCTGCAGTTGAAACAGATAAAACAAAACGCGCAGAACTA 1560 2 1270 .......................................................................................... 1359 1 385 .......................................................................................... 296 Y H K I Q E T A R E D L P Y L P I A Y P K A V I A V D K K F Query_1 1561 TATCATAAAATTCAAGAGACAGCTAGAGAAGACTTACCTTATCTACCAATTGCGTATCCGAAAGCAGTTATTGCAGTAGATAAAAAGTTT 1650 2 1360 .......................................................................................... 1449 1 295 .......................................................................................... 206 0 1027 ........... 1037 D G L K E A K A I P V T M F E D V S K I Y E V K * H W K W I Query_1 1651 GATGGATTGAAAGAAGCAAAAGCAATTCCTGTCACAATGTTTGAAGATGTATCTAAGATTTATGAAGTGAAATAACATTGGAAGTGGATA 1740 2 1450 ........................................................................ 1521 1 205 .......................................................................................... 116 T D P N S S S V D K L A A A L E H H H H H H * D P A A N K Query_1 1741 ACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAG-C 1829 1 115 ........................................................................................C. 26 A R Query_1 1830 CCGAAA 1835 1 25 ...... 20 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 7389739 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 3,922 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP05180.102 (IDP05180.24-568.P(568)..pMCSG68)
ACSGSGGEKKSTTTSSGGGEEKKSEIKYAAKQVLNRTENQEIPTMDVSKSTDTLGSQILGNTMEGLYRLDKDNKPIPAAAESSTKSEDGKKYTFKLRKDAKWSNGDPVTAKDFVYAWQRLLDKNTAAEYAFIAYYIKNAEAINKGEKPLTDLGAKAVDDYTLEVELEKPVPYFLNLMAFPSYYPLNEKFVKEKGDKFGLEADTTLYNGPFVMASWKHEQGWQLKKNDKYWDNKTVKLEEINYSVVKEVATKVNLYDTGSIDFTLLSGEFVDKYKSNKEEYGEYSEASTFFLRLNQKRNGQDTPLKSKKLREAIALSIDKKGLATVILNNGSKATDQLVPKGLATGPDGKDYQDTFKNGLKYDPKKGAAAWEEAKKELGKDQVTIELLSYDDGTAKKIADYFKDQIEKNLKGVTVNTKIQPFKQKLKLESAQDYEVSFAGWSPDYSDPMTFIDMFESKSPYNQMSYSNPKYDEMVAKAGNELLSDPKKRWETLGKAEKLFLEEDAGLVPLYQTGRAYVMKPNVKGIVKHNISPEYSFKWAYVTEGK
GCTTGTTCTGGATCTGGTGGGGAGAAGAAATCAACTACGACGTCTAGTGGTGGTGGGGAAGAGAAAAAGTCTGAAATTAAATACGCAGCGAAACAAGTGTTAAATCGTACAGAGAATCAAGAGATTCCGACGATGGATGTTTCAAAATCTACCGATACATTAGGTTCTCAAATTTTAGGGAACACGATGGAAGGTTTATATCGATTAGATAAAGATAATAAGCCAATCCCAGCTGCAGCAGAATCTAGTACGAAAAGCGAGGATGGCAAAAAATATACATTTAAATTACGTAAAGATGCAAAATGGTCAAATGGTGATCCTGTAACAGCGAAAGATTTCGTATATGCATGGCAGCGCTTACTTGATAAAAATACAGCGGCAGAATATGCATTTATTGCTTACTATATTAAAAACGCAGAGGCAATTAATAAAGGTGAAAAACCACTAACAGATTTAGGAGCAAAAGCGGTAGATGATTATACGCTAGAAGTAGAATTAGAGAAACCAGTACCATATTTCTTGAATTTAATGGCATTCCCATCTTACTATCCTTTAAATGAAAAGTTCGTAAAAGAAAAAGGAGATAAATTCGGTTTAGAAGCAGATACAACGTTGTATAACGGACCGTTCGTTATGGCTTCATGGAAACATGAACAAGGATGGCAGCTAAAGAAAAATGATAAGTACTGGGATAATAAGACTGTAAAATTAGAAGAAATTAACTATAGTGTAGTAAAAGAAGTTGCGACGAAAGTAAACTTATATGATACAGGATCAATTGATTTCACGTTATTATCAGGAGAATTCGTTGATAAATATAAATCGAACAAAGAAGAGTACGGCGAGTATTCGGAAGCAAGTACATTCTTCTTACGTTTAAATCAAAAGCGTAACGGACAAGATACACCGTTAAAGAGCAAAAAACTTCGTGAAGCGATCGCATTATCAATTGATAAAAAAGGATTAGCAACCGTTATTTTAAATAACGGTTCAAAAGCAACAGATCAATTAGTACCAAAAGGGCTTGCGACAGGACCAGACGGTAAAGACTACCAAGATACGTTTAAAAATGGTCTAAAATATGATCCGAAAAAAGGTGCAGCAGCTTGGGAAGAAGCGAAAAAAGAACTTGGAAAAGATCAAGTGACAATTGAATTACTAAGCTATGATGATGGAACTGCGAAAAAAATTGCTGACTACTTTAAAGATCAAATTGAGAAAAACTTAAAAGGTGTAACGGTTAACACGAAAATTCAACCGTTCAAACAAAAACTAAAATTAGAGTCAGCACAAGATTATGAAGTTTCGTTTGCAGGTTGGAGTCCAGATTATTCGGATCCAATGACATTTATTGATATGTTTGAATCGAAGAGCCCATATAACCAAATGAGTTATTCGAATCCAAAATATGATGAAATGGTAGCGAAAGCAGGTAATGAATTACTGTCTGATCCGAAGAAGCGTTGGGAAACGTTAGGAAAAGCAGAGAAATTATTCCTTGAAGAAGATGCAGGATTAGTTCCTTTATATCAAACAGGAAGAGCGTATGTAATGAAACCGAATGTAAAAGGAATTGTGAAACATAACATTAGTCCAGAATATAGCTTTAAGTGGGCTTATGTAACGGAAGGTAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,408 total letters Query= IDP05180.102 Length=2040 Score E Sequences producing significant alignments: (Bits) Value wt 2949 0.0 08A_A2_IDP05180.102_T7term.seq 1287 0.0 A C S G S G G E K K S T T T S S G G G E E K K S E I K Y A A Query_1 202 GCTTGTTCTGGATCTGGTGGGGAGAAGAAATCAACTACGACGTCTAGTGGTGGTGGGGAAGAGAAAAAGTCTGAAATTAAATACGCAGCG 291 2 1 .......................................................................................... 90 K Q V L N R T E N Q E I P T M D V S K S T D T L G S Q I L G Query_1 292 AAACAAGTGTTAAATCGTACAGAGAATCAAGAGATTCCGACGATGGATGTTTCAAAATCTACCGATACATTAGGTTCTCAAATTTTAGGG 381 2 91 .......................................................................................... 180 N T M E G L Y R L D K D N K P I P A A A E S S T K S E D G K Query_1 382 AACACGATGGAAGGTTTATATCGATTAGATAAAGATAATAAGCCAATCCCAGCTGCAGCAGAATCTAGTACGAAAAGCGAGGATGGCAAA 471 2 181 .......................................................................................... 270 K Y T F K L R K D A K W S N G D P V T A K D F V Y A W Q R L Query_1 472 AAATATACATTTAAATTACGTAAAGATGCAAAATGGTCAAATGGTGATCCTGTAACAGCGAAAGATTTCGTATATGCATGGCAGCGCTTA 561 2 271 .......................................................................................... 360 L D K N T A A E Y A F I A Y Y I K N A E A I N K G E K P L T Query_1 562 CTTGATAAAAATACAGCGGCAGAATATGCATTTATTGCTTACTATATTAAAAACGCAGAGGCAATTAATAAAGGTGAAAAACCACTAACA 651 2 361 .......................................................................................... 450 D L G A K A V D D Y T L E V E L E K P V P Y F L N L M A F P Query_1 652 GATTTAGGAGCAAAAGCGGTAGATGATTATACGCTAGAAGTAGAATTAGAGAAACCAGTACCATATTTCTTGAATTTAATGGCATTCCCA 741 2 451 .......................................................................................... 540 S Y Y P L N E K F V K E K G D K F G L E A D T T L Y N G P F Query_1 742 TCTTACTATCCTTTAAATGAAAAGTTCGTAAAAGAAAAAGGAGATAAATTCGGTTTAGAAGCAGATACAACGTTGTATAACGGACCGTTC 831 2 541 .......................................................................................... 630 2 618 .............. 605 V M A S W K H E Q G W Q L K K N D K Y W D N K T V K L E E I Query_1 832 GTTATGGCTTCATGGAAACATGAACAAGGATGGCAGCTAAAGAAAAATGATAAGTACTGGGATAATAAGACTGTAAAATTAGAAGAAATT 921 2 631 .......................................................................................... 720 N Y S V V K E V A T K V N L Y D T G S I D F T L L S G E F V Query_1 922 AACTATAGTGTAGTAAAAGAAGTTGCGACGAAAGTAAACTTATATGATACAGGATCAATTGATTTCACGTTATTATCAGGAGAATTCGTT 1011 2 721 .......................................................................................... 810 D K Y K S N K E E Y G E Y S E A S T F F L R L N Q K R N G Q Query_1 1012 GATAAATATAAATCGAACAAAGAAGAGTACGGCGAGTATTCGGAAGCAAGTACATTCTTCTTACGTTTAAATCAAAAGCGTAACGGACAA 1101 2 811 .......................................................................................... 900 D T P L K S K K L R E A I A L S I D K K G L A T V I L N N G Query_1 1102 GATACACCGTTAAAGAGCAAAAAACTTCGTGAAGCGATCGCATTATCAATTGATAAAAAAGGATTAGCAACCGTTATTTTAAATAACGGT 1191 2 901 .......................................................................................... 990 2 991 ...........A.......... 970 2 956 .............. 943 S K A T D Q L V P K G L A T G P D G K D Y Q D T F K N G L Query_1 1192 TCAAAAGCAACAGATCAATTAGTACCAAAAGGG-CTTGCGACAGGACCAGACGGTAAAGACTACCAAGATACGTTTAAAAATGGTCTAAA 1280 2 991 .................................-........................................................ 1079 2 969 . 969 1 768 .....N.............G....N.....................................T........A.... 693 K Y D P K K G A A A W E E A K K E L G K D Q V T I E L L S Y Query_1 1281 ATATGATCCGAAAAAAGGTGCAGCAGCTTGGGAAGAAGCGAAAAAA-GAACTTGGAAAAGATCAAGTGACAATTGAATTACTAAGCTATG 1369 2 1080 ..............................................-........................................... 1168 1 692 ........N.....................................A.........................................N. 603 D D G T A K K I A D Y F K D Q I E K N L K G V T V N T K I Q Query_1 1370 ATGATGGAACTGCGaaaaaaaTTGCTGACTACTTTAAAGATCAAATTGAGAAAAACTTAAAAGGTGTAACGGTTAACACGAAAATTCAAC 1459 2 1169 .......................................................................................... 1258 1 602 .N........................................................................................ 513 P F K Q K L K L E S A Q D Y E V S F A G W S P D Y S D P M T Query_1 1460 CGTTCAAACAAAAACTAAAATTAGAGTCAGCACAAGATTATGAAGTTTCGTTTGCAGGTTGGAGTCCAGATTATTCGGATCCAATGACAT 1549 2 1259 .......................................................................................... 1348 1 512 .......................................................................................... 423 F I D M F E S K S P Y N Q M S Y S N P K Y D E M V A K A G N Query_1 1550 TTATTGATATGTTTGAATCGAAGAGCCCATATAACCAAATGAGTTATTCGAATCCAAAATATGATGAAATGGTAGCGAAAGCAGGTAATG 1639 2 1349 .......................................................................................... 1438 1 422 .......................................................................................... 333 E L L S D P K K R W E T L G K A E K L F L E E D A G L V P L Query_1 1640 AATTACTGTCTGATCCGAAGAAGCGTTGGGAAACGTTAGGAAAAGCAGAGAAATTATTCCTTGAAGAAGATGCAGGATTAGTTCCTTTAT 1729 2 1439 .......................................................................................... 1528 1 332 .......................................................................................... 243 Y Q T G R A Y V M K P N V K G I V K H N I S P E Y S F K W A Query_1 1730 ATCAAACAGGAAGAGCGTATGTAATGAAACCGAATGTAAAAGGAATTGTGAAACATAACATTAGTCCAGAATATAGCTTTAAGTGGGCTT 1819 2 1529 .......................................................................................... 1618 1 242 ..................................N....................................................... 153 Y V T E G K * H W K W I T D P N S S S V D K L A A A L E H H Query_1 1820 ATGTAACGGAAGGTAAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccacc 1909 2 1619 ................. 1635 1 152 .......................................................................................... 63 H H H H * D P A A N K A R K E Query_1 1910 accaccaccacTGAGATCCGGCTGCTAACAAA--GCCCGAAAGGAA 1953 1 62 ..........N.....................AGN.......A... 17 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4776640 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 2,408 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP05387.102 (IDP05387.21-553.P(553)..pMCSG68)
GCGNGEKASTTKKEAGKGAADKQVLNLLETAEIPSMDTSKSTDSVSFRAFVNAMEGLYRLDKDNKPTPGMAKDVKISEDKKIYTFELRDAKWSNGDPVRAQDFVYAWQRTLDKATAAEYAFILFDVKNAQKVNKGELPLDQLGVKAKDDKTLVVELEQPAPYFLELTSFPTFFPLNEKYVKEQGDKYALEADKLLYNGPFVMSEWKHERSYQLKKNPNYWDKDTVKLEEINVNIVKETSTGVNLYDSDQADRVILSSEFVDKYKKNKPDEFKTKLDPRMYFLRFNQKNATFKNQKVREAIDLAYDKKGIANVILNDGSLPAYFLVPEKFTTGPDGKDFRSVNKNFRDSKDNAAKAKKLWEEAKKELGQDTITVELLNDDNGSRKKVGDFIKEELEKNLPGIKVNLKQQPYKQKLDLEKKFEYEFSLSAWSPDYPDPMTYIDMFVTGSSFNEMDYSNPKYDELVAKSKGELLKDDKARWNALAEAEKILIGQDAAISPTYQQSTAYLEKPYVKGIANHTFGGDFSYKWAYITKK
GGTTGCGGAAATGGGGAGAAGGCATCTACAACGAAGAAAGAAGCAGGTAAGGGAGCAGCGGATAAGCAAGTATTAAACTTACTAGAAACAGCAGAAATTCCTTCAATGGATACGTCAAAATCAACAGACTCTGTATCATTCCGTGCCTTTGTAAATGCAATGGAAGGATTATATCGTTTAGATAAGGATAATAAACCAACTCCAGGTATGGCAAAAGATGTGAAAATTAGTGAAGATAAAAAAATATATACATTTGAATTACGAGACGCAAAGTGGTCTAATGGCGATCCAGTTCGAGCGCAAGATTTTGTATATGCATGGCAACGTACATTAGATAAAGCAACGGCAGCTGAGTATGCATTTATTTTATTTGATGTAAAGAATGCACAGAAAGTCAATAAAGGAGAATTACCGTTAGATCAATTAGGGGTGAAAGCGAAGGATGATAAAACATTAGTAGTAGAATTAGAACAACCAGCACCATATTTCCTTGAGCTTACTTCATTCCCAACATTTTTCCCTTTAAATGAAAAATATGTGAAAGAACAAGGGGATAAATATGCATTAGAAGCAGATAAACTATTATACAATGGCCCATTTGTAATGAGTGAATGGAAACATGAACGAAGTTATCAGTTAAAGAAAAATCCTAATTATTGGGATAAAGATACTGTGAAATTAGAAGAAATTAATGTGAATATTGTAAAGGAAACGAGCACGGGAGTAAATTTATATGATAGTGATCAAGCAGACCGAGTTATTTTAAGTTCTGAATTTGTTGATAAATATAAAAAGAATAAACCAGATGAATTTAAAACGAAGTTAGATCCGCGTATGTATTTCTTACGCTTTAACCAGAAGAATGCAACGTTTAAAAACCAAAAAGTTCGTGAAGCGATTGATCTGGCATATGATAAAAAAGGAATTGCAAATGTTATTTTAAATGATGGATCATTGCCAGCATACTTCCTAGTACCTGAAAAATTTACGACAGGACCAGATGGGAAAGATTTCCGTTCTGTAAATAAAAATTTCCGTGACAGTAAAGATAATGCAGCAAAAGCAAAAAAACTATGGGAAGAAGCGAAGAAAGAACTTGGTCAAGATACAATCACAGTAGAATTACTAAATGATGACAACGGTAGTCGGAAAAAAGTAGGAGACTTTATTAAAGAAGAGTTAGAGAAAAACTTACCTGGTATTAAAGTGAATTTAAAACAACAGCCATATAAGCAAAAGTTAGATTTAGAAAAGAAATTTGAATATGAATTCTCTTTATCTGCATGGAGTCCAGATTATCCAGATCCAATGACTTATATTGATATGTTCGTTACGGGAAGCTCATTTAATGAAATGGATTATTCTAATCCGAAGTATGATGAGTTAGTAGCGAAATCAAAAGGAGAGCTATTAAAAGATGATAAAGCACGCTGGAATGCACTAGCAGAAGCTGAAAAAATCTTAATTGGTCAAGATGCTGCCATTTCTCCTACGTACCAACAAAGTACCGCATACTTAGAGAAACCATACGTAAAAGGTATTGCAAATCATACGTTTGGTGGGGACTTCAGTTATAAATGGGCATATATTACAAAAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,165 total letters Query= IDP05387.102 Length=2004 Score E Sequences producing significant alignments: (Bits) Value wt 2884 0.0 01G_G1_IDP05387.102_T7short.seq 69.8 8e-15 07G_G1_IDP05387.102_T7term.seq 39.2 1e-05 Q F P S R N N F V * L * E G D I H M H H H H H H S S G V D Query_1 55 CAATTCCCCT-CTAGAAATAATTTTGTTTAACTTT-AAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATC 142 2 865 ............ 854 0 15 ..T.......T..N...-.................T....................A.. 72 L W S H P Q F E K G T E N L Y F Q S N A G C G N G E K A S T Query_1 143 TGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGGTTGCGGAAATGGGGAGAAGGCATCTACAA 232 2 1 ............................... 31 T K K E A G K G A A D K Q V L N L L E T A E I P S M D T S K Query_1 233 CGAAGAAAGAAGCAGGTAAGGGAGCAGCGGATAAGCAAGTATTAAACTTACTAGAAACAGCAGAAATTCCTTCAATGGATACGTCAAAAT 322 2 32 .......................................................................................... 121 2 1085 ........... 1095 S T D S V S F R A F V N A M E G L Y R L D K D N K P T P G M Query_1 323 CAACAGACTCTGTATCATTCCGTGCCTTTGTAAATGCAATGGAAGGATTATATCGTTTAGATAAGGATAATAAACCAACTCCAGGTATGG 412 2 122 .......................................................................................... 211 A K D V K I S E D K K I Y T F E L R D A K W S N G D P V R A Query_1 413 CAAAAGATGTGAAAATTAGTGAAGATaaaaaaaTATATACATTTGAATTACGAGACGCAAAGTGGTCTAATGGCGATCCAGTTCGAGCGC 502 2 212 .......................................................................................... 301 Q D F V Y A W Q R T L D K A T A A E Y A F I L F D V K N A Q Query_1 503 AAGATTTTGTATATGCATGGCAACGTACATTAGATAAAGCAACGGCAGCTGAGTATGCATTTATTTTATTTGATGTAAAGAATGCACAGA 592 2 302 .......................................................................................... 391 K V N K G E L P L D Q L G V K A K D D K T L V V E L E Q P A Query_1 593 AAGTCAATAAAGGAGAATTACCGTTAGATCAATTAGGGGTGAAAGCGAAGGATGATAAAACATTAGTAGTAGAATTAGAACAACCAGCAC 682 2 392 .......................................................................................... 481 P Y F L E L T S F P T F F P L N E K Y V K E Q G D K Y A L E Query_1 683 CATATTTCCTTGAGCTTACTTCATTCCCAACATTTTTCCCTTTAAATGAAAAATATGTGAAAGAACAAGGGGATAAATATGCATTAGAAG 772 2 482 .......................................................................................... 571 1 318 ...........C.. 305 A D K L L Y N G P F V M S E W K H E R S Y Q L K K N P N Y W Query_1 773 CAGATAAACTATTATACAATGGCCCATTTGTAATGAGTGAATGGAAACATGAACGAAGTTATCAGTTAAAGAAAAATCCTAATTATTGGG 862 2 572 .......................................................................................... 661 D K D T V K L E E I N V N I V K E T S T G V N L Y D S D Q A Query_1 863 ATAAAGATACTGTGAAATTAGAAGAAATTAATGTGAATATTGTAAAGGAAACGAGCACGGGAGTAAATTTATATGATAGTGATCAAGCAG 952 2 662 .......................................................................................... 751 D R V I L S S E F V D K Y K K N K P D E F K T K L D P R M Y Query_1 953 ACCGAGTTATTTTAAGTTCTGAATTTGTTGATAAATATAAAAAGAATAAACCAGATGAATTTAAAACGAAGTTAGATCCGCGTATGTATT 1042 2 752 .......................................................................................... 841 2 1208 ............ 1219 F L R F N Q K N A T F K N Q K V R E A I D L A Y D K K G I A Query_1 1043 TCTTACGCTTTAACCAGAAGAATGCAACGTTTAAAAACCAAAAAGTTCGTGAAGCGATTGATCTGGCATATGATAAAAAAGGAATTGCAA 1132 2 842 .......................................................................................... 931 N V I L N D G S L P A Y F L V P E K F T T G P D G K D F R S Query_1 1133 ATGTTATTTTAAATGATGGATCATTGCCAGCATACTTCCTAGTACCTGAAAAATTTACGACAGGACCAGATGGGAAAGATTTCCGTTCTG 1222 2 932 .......................................................................................... 1021 V N K N F R D S K D N A A K A K K L W E E A K K E L G Q D T Query_1 1223 TAAATAAAAATTTCCGTGACAGTAAAGATAATGCAGCAAAAGCaaaaaaaCTATGGGAAGAAGCGAAGAAAGAACTTGGTCAAGATACAA 1312 2 1022 .......................................................................................... 1111 2 32 ........... 42 2 1466 ........... 1476 I T V E L L N D D N G S R K K V G D F I K E E L E K N L P G Query_1 1313 TCACAGTAGAATTACTAAATGATGACAACGGTAGTCGGAAAAAAGTAGGAGACTTTATTAAAGAAGAGTTAGAGAAAAACTTACCTGGTA 1402 2 1112 .......................................................................................... 1201 I K V N L K Q Q P Y K Q K L D L E K K F E Y E F S L S A W S Query_1 1403 TTAAAGTGAATTTAAAACAACAGCCATATAAGCAAAAGTTAGATTTAGAAAAGAAATTTGAATATGAATTCTCTTTATCTGCATGGAGTC 1492 2 1202 .......................................................................................... 1291 2 807 ............ 818 P D Y P D P M T Y I D M F V T G S S F N E M D Y S N P K Y D Query_1 1493 CAGATTATCCAGATCCAATGACTTATATTGATATGTTCGTTACGGGAAGCTCATTTAATGAAATGGATTATTCTAATCCGAAGTATGATG 1582 2 1292 .......................................................................................... 1381 E L V A K S K G E L L K D D K A R W N A L A E A E K I L I G Query_1 1583 AGTTAGTAGCGAAATCAAAAGGAGAGCTATTAAAAGATGATAAAGCACGCTGGAATGCACTAGCAGAAGCTGAAAAAATCTTAATTGGTC 1672 2 1382 .......................................................................................... 1471 2 1097 ...... 1102 Q D A A I S P T Y Q Q S T A Y L E K P Y V K G I A N H T F G Query_1 1673 AAGATGCTGCCATTTCTCCTACGTACCAACAAAGTACCGCATACTTAGAGAAACCATACGTAAAAGGTATTGCAAATCATACGTTTGGTG 1762 2 1472 .......................................................................................... 1561 2 1103 ..... 1107 G D F S Y K W A Y I T K K * H W K W I T D P N S S S V D K L Query_1 1763 GGGACTTCAGTTATAAATGGGCATATATTACaaaaaaaTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTG 1852 2 1562 ...................................... 1599 1 67 ...................--..... 44 A A A L E H H H H H H * D P A A N K A R Query_1 1853 CGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAG-CCCGAAA 1913 1 48 ..................C....... 23 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 8174518 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 4,165 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP05404.102 (IDP05404.22-314.P(314)..pMCSG68)
ACSNKEEKKADAKDAKTEERTVQHAKGEIKIPANPKKIADLSGSTEELLIFGMKPIITANTSQEKIDAHIEKKLKDVKPVGSAWGDKINIEAVAAAKPDLILVNNRQEKIYDQLSKIAPTVMLKTPLDQWRPKFEEVGQIFGKEKETKEWFKQYDEKASKLHDKIVAKTGDAKFMKMAAYPNAFRVYGDYGYGSVIFNDLKLPAVKGTPTDKPLVQVQKEALIDYNPDYLFVFTTGDGSQRLKEFQEESIWKNMNAVKNNHVFTIKNEELNKGYFPLGKEMILDEVAEFVLGK
GCTTGTTCTAACAAAGAAGAAAAGAAAGCAGATGCGAAGGATGCGAAGACTGAAGAGCGCACTGTACAACATGCAAAAGGGGAAATTAAAATCCCTGCAAATCCAAAGAAAATTGCTGACCTTAGTGGTTCAACAGAAGAATTATTAATCTTTGGTATGAAACCAATTATTACTGCTAATACATCTCAAGAAAAAATTGATGCACATATTGAAAAGAAATTAAAAGACGTAAAACCAGTTGGTTCTGCTTGGGGCGATAAAATTAACATCGAAGCAGTAGCTGCTGCAAAACCAGACTTAATTCTTGTAAACAATCGTCAAGAAAAGATTTATGATCAATTATCTAAAATTGCACCAACAGTTATGTTAAAAACTCCATTAGACCAATGGCGTCCAAAATTCGAAGAAGTAGGTCAAATCTTCGGTAAAGAGAAAGAAACAAAAGAATGGTTCAAACAGTATGATGAAAAAGCAAGCAAATTACATGACAAAATCGTTGCGAAAACTGGCGATGCTAAATTCATGAAAATGGCTGCATATCCAAATGCTTTCCGTGTATATGGTGACTACGGTTACGGTAGCGTAATCTTTAATGATTTAAAATTACCAGCAGTTAAAGGTACACCAACTGATAAACCGTTAGTACAAGTGCAAAAAGAAGCTTTAATTGATTACAACCCAGATTACTTATTCGTATTTACGACTGGTGACGGTTCTCAGCGCCTAAAAGAATTCCAAGAAGAATCAATTTGGAAAAATATGAACGCTGTTAAAAACAACCACGTATTTACAATTAAAAACGAAGAATTAAACAAAGGTTACTTCCCACTAGGTAAAGAAATGATCTTAGATGAAGTTGCTGAGTTCGTTTTAGGGAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,450 total letters Query= IDP05404.102 Length=1284 Score E Sequences producing significant alignments: (Bits) Value wt 1586 0.0 08C_C2_IDP05404.102_T7term.seq 1548 0.0 02C_C2_IDP05404.102_T7short.seq 985 0.0 G * Q F P S R N N F V * L * E G D I H M H H H H H H S Query_1 47 GCGGATAACAATT-CCCCTCT--AGAAATAATTTTG--TTTAACTTT-AAGAAGGAGATATACATATGCACCATCATCATCATCATTCTT 130 0 7 ....-.....T..T.....T.TANN....N.....TGT.........T..N........................---............ 92 S G V D L W S H P Q F E K G T E N L Y F Q S N A A C S N K E Query_1 131 CTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGCTTGTTCTAACAAAGAAG 220 2 1 ................... 19 0 93 ......................................................................................N... 182 E K K A D A K D A K T E E R T V Q H A K G E I K I P A N P K Query_1 221 AAAAGAAAGCAGATGCGAAGGATGCGAAGACTGAAGAGCGCACTGTACAACATGCAAAAGGGGAAATTAAAATCCCTGCAAATCCAAA-G 309 2 20 ........................................................................................-. 108 1 964 .....N.......C..................N..-.........TC.....A. 912 0 183 ........................................................................................-. 271 0 27 ........... 17 K I A D L S G S T E E L L I F G M K P I I T A N T S Q E Query_1 310 AAAATTGCTGACCTTAGTGGTTCAAC-AGAA-GAA-TTATTAATCTTTGGTATGAAACCAATTATT-ACTGCTAATACATCTCAAGAA-A 394 2 109 ..........................-....-...-..............................-.....................-. 193 1 911 ............N.N.....N.....C....A...N...........N.-....N..N........T...................G.G. 823 0 272 ..........................-....-...-..............................-.....................-. 356 K I D A H I E K K L K D V K P V G S A W G D K I N I E A V A Query_1 395 AAATTGATGCACATATTGAAAAGAAATTAAAAGACGTAAAACCAGTTGGTTCTGCTTGGGGCGATAAAATTAACATCGAAGCAGTAGCTG 484 2 194 .......................................................................................... 283 1 822 ...N...........N.................N.................N...................................... 733 0 357 .......................................................................................... 446 A A K P D L I L V N N R Q E K I Y D Q L S K I A P T V M L K Query_1 485 CTGCAAAACCAGACTTAATTCTTGTAAACAATCGTCAAGAAAAGATTTATGATCAATTATCTAAAATTGCACCAACAGTTATGTTAAAAA 574 2 284 .......................................................................................... 373 1 732 ....................N..................................................................... 643 0 447 .........................................................................NN............... 536 T P L D Q W R P K F E E V G Q I F G K E K E T K E W F K Q Y Query_1 575 CTCCATTAGACCAATGGCGTCCAAAATTCGAAGAAGTAGGTCAAATCTTCGGTAAAGAGAAAGAAACAAAAGAATGGTTCAAACAGTATG 664 2 374 .......................................................................................... 463 1 642 .......................................................................................... 553 0 537 .............................N......................................... 607 D E K A S K L H D K I V A K T G D A K F M K M A A Y P N A F Query_1 665 ATGAAAAAGCAAGCAAATTACATGACAAAATCGTTGCGAAAACTGGCGATGCTAAATTCATGAAAATGGCTGCATATCCAAATGCTTTCC 754 2 464 .......................................................................................... 553 1 552 .........................................................................................N 463 R V Y G D Y G Y G S V I F N D L K L P A V K G T P T D K P Query_1 755 GTGTATATGGTGACTACGGTTACGGTAGCGTAATCTTTAATGATTTAAAATT--ACCAGCAGTTAAAGGTACACCAACTGATAAACCGTT 842 2 554 ....................................................--.................................... 641 1 462 .............N......................................--.................................... 375 0 501 ...T..C.......GC...NN...... 527 L V Q V Q K E A L I D Y N P D Y L F V F T T G D G S Q R L K Query_1 843 AGTACAAGTGCAAAAAGAAGCTTTAATTGATTACAACCCAGATTACTTATTCGTATTTACGACTGGTGACGGTTCTCAGCGCCTAAAAGA 932 2 642 .......................................................................................... 731 1 374 .......................................................................................... 285 0 561 . 561 E F Q E E S I W K N M N A V K N N H V F T I K N E E L N K G Query_1 933 ATTCCAAGAAGAATCAATTTGGAAAAATATGAACGCTGTTAAAAACAACCACGTATTTACAATTAAAAACGAAGAATTAAACAAAGGTTA 1022 2 732 .......................................................................................... 821 1 284 .......................................................................................... 195 0 562 ....N...... 572 Y F P L G K E M I L D E V A E F V L G K * H W K W I T D P N Query_1 1023 CTTCCCACTAGGTAAAGAAATGATCTTAGATGAAGTTGCTGAGTTCGTTTTAGGGAAATAACATTGGAAGTGGATAACGGATCCGAATTC 1112 2 822 .......................................................... 879 1 194 .......................................................................................... 105 S S S V D K L A A A L E H H H H H H * D P A A N K A R Query_1 1113 GAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTG-AGA-TCCGGCTGCTAACAAAGCCCGAAA 1193 1 104 ......................................................A...N...................N.... 22 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3051945 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 2,450 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP05599.102 (IDP05599.28-568.P(568)..pMCSG68)
SSGGEKKSTTTSNNGGEEKKSDIKYAAKQVLNRTETNEIPTMDTSKNTDTLGSQILGNTMEGLYRLDKNNKPIPAVAESSTKSEDGKKYTFKLRKDAKWSNGDPVTAKDFVFAWQRLVDPKKAAEYAFIAYYIKNAETINQGKGEVSTLGVKAVDDYTLEVELERPVPYFLNLMAFASYYPLNEKFVKEKGNKFGLESDTTLYNGPFVLTDWKHEQGWKLKKNEQYWDKKTVKLEEINYSVVKEPATRVNLYDTGAIDFTLLSGEFVDKYRNNKEEFGAYSETSTFYLRLNQKRGGQDTPLKSKKLREAIALSIDKKALTNVILNDGSKPVDYLVPKGLASGPDGKDFAETFKNGLKQDSKKAAAAWEEAKKELGKDQVTIELLNYDTGNAKKVGEYVKDQVEKNLKGVTVNIKLQPFKQKLKLESDQDYDFSYGGWNPDYADPMTYLDMFETKNSQNQMSYSNSKYDDIITKSKTEWMADAKKRWTELGKAEKLLLEEDVALVPLYQTARSYVMKPNIKGIVKHNISPEYSFKWAYVEEK
TCTTCTGGTGGGGAGAAGAAATCGACTACAACGTCTAATAATGGTGGGGAAGAGAAGAAGTCTGATATTAAATATGCGGCGAAGCAAGTGTTAAATCGTACAGAAACGAATGAAATTCCGACGATGGATACTTCCAAAAATACAGATACACTTGGCTCACAAATTTTAGGGAATACAATGGAAGGGTTATATCGCCTTGATAAAAACAATAAGCCAATCCCAGCTGTAGCAGAATCTAGCACAAAAAGCGAGGATGGTAAAAAATATACATTTAAACTACGTAAAGATGCAAAATGGTCAAACGGTGATCCAGTAACAGCGAAAGATTTCGTATTTGCATGGCAACGTCTAGTAGATCCAAAAAAAGCTGCTGAGTATGCATTTATCGCTTACTATATTAAAAATGCGGAAACAATTAATCAAGGAAAAGGAGAAGTTTCTACATTAGGTGTAAAAGCGGTAGATGATTATACGCTTGAAGTGGAACTAGAAAGACCAGTACCATATTTCTTGAACTTAATGGCATTTGCGTCTTACTATCCATTAAATGAAAAGTTTGTGAAAGAAAAAGGGAATAAATTCGGTTTAGAGTCTGATACAACACTTTATAATGGACCATTCGTGCTTACTGATTGGAAGCATGAGCAAGGTTGGAAATTAAAGAAAAATGAGCAGTATTGGGACAAAAAGACTGTCAAACTAGAAGAAATCAATTATAGTGTAGTAAAAGAACCAGCTACTAGAGTAAATTTATATGACACAGGTGCGATTGATTTCACACTTTTATCAGGTGAATTTGTTGATAAGTATAGAAATAATAAAGAAGAATTTGGTGCATATTCGGAAACAAGTACGTTTTATTTACGTCTAAACCAAAAACGTGGTGGGCAAGATACACCGTTAAAGAGCAAAAAACTACGTGAAGCAATTGCATTATCAATTGATAAAAAAGCTTTAACGAATGTTATTTTAAATGATGGTTCAAAACCAGTGGATTATTTAGTACCAAAAGGTTTAGCGAGTGGACCAGACGGTAAAGATTTCGCAGAAACGTTCAAAAATGGTTTAAAACAAGACTCCAAAAAGGCAGCGGCAGCCTGGGAAGAAGCGAAAAAAGAACTTGGAAAAGATCAAGTCACAATTGAACTGTTAAACTATGATACTGGTAATGCGAAAAAAGTTGGGGAGTATGTAAAAGACCAAGTTGAAAAGAATTTAAAAGGTGTAACAGTAAATATTAAACTGCAGCCATTTAAGCAAAAACTAAAATTAGAATCAGACCAAGATTATGATTTCTCATATGGCGGCTGGAATCCAGATTATGCGGATCCAATGACATACCTTGATATGTTTGAAACAAAAAATTCGCAGAACCAGATGAGCTACTCAAATTCAAAATATGATGACATTATTACTAAAAGTAAGACAGAATGGATGGCTGATGCGAAAAAACGTTGGACAGAGTTAGGGAAAGCAGAAAAATTGTTACTTGAAGAAGATGTAGCGCTTGTGCCTTTATATCAAACTGCTAGATCATATGTTATGAAACCAAATATAAAGGGAATTGTGAAACATAATATTAGTCCGGAATATAGCTTTAAATGGGCGTATGTAGAAGAGAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,279 total letters Query= IDP05599.102 Length=2028 Score E Sequences producing significant alignments: (Bits) Value wt 2928 0.0 08B_B2_IDP05599.102_T7term.seq 1521 0.0 02B_B2_IDP05599.102_T7short.seq 1223 0.0 G I V S G * Q F P S R N N F V * L * E G D I H M H H H H H Query_1 36 GGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTT--GTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCAT 123 2 1560 ........... 1570 1 203 ........... 193 0 21 ...T.T.N.....N.....TT....N........................................ 86 H S S G V D L W S H P Q F E K G T E N L Y F Q S N A S S G G Query_1 124 CATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCTCTTCTGGTGGG 213 2 1 ............ 12 0 87 .......................................................................................... 176 E K K S T T T S N N G G E E K K S D I K Y A A K Q V L N R T Query_1 214 GAGAAGAAATCGACTACAACGTCTAATAATGGTGGGGAAGAGAAGAAGTCTGATATTAAATATGCGGCGAAGCAAGTGTTAAATCGTACA 303 2 13 .......................................................................................... 102 0 177 .......................................................................................... 266 E T N E I P T M D T S K N T D T L G S Q I L G N T M E G L Y Query_1 304 GAAACGAATGAAATTCCGACGATGGATACTTCCAAAAATACAGATACACTTGGCTCACAAATTTTAGGGAATACAATGGAAGGGTTATAT 393 2 103 .......................................................................................... 192 0 267 .......................................................................................... 356 R L D K N N K P I P A V A E S S T K S E D G K K Y T F K L R Query_1 394 CGCCTTGATAAAAACAATAAGCCAATCCCAGCTGTAGCAGAATCTAGCACAAAAAGCGAGGATGGTAAAAAATATACATTTAAACTACGT 483 2 193 .......................................................................................... 282 0 357 .......................................................................................... 446 K D A K W S N G D P V T A K D F V F A W Q R L V D P K K A A Query_1 484 AAAGATGCAAAATGGTCAAACGGTGATCCAGTAACAGCGAAAGATTTCGTATTTGCATGGCAACGTCTAGTAGATCCaaaaaaaGCTGCT 573 2 283 .......................................................................................... 372 0 447 .......................................................................................... 536 E Y A F I A Y Y I K N A E T I N Q G K G E V S T L G V K A V Query_1 574 GAGTATGCATTTATCGCTTACTATATTAAAAATGCGGAAACAATTAATCAAGGAAAAGGAGAAGTTTCTACATTAGGTGTAAAAGCGGTA 663 2 373 .......................................................................................... 462 0 537 .......................................................................................... 626 D D Y T L E V E L E R P V P Y F L N L M A F A S Y Y P L N Query_1 664 GATGATTATACGCTTGAAGTGGAACTAGAAAGACCAGTACCATATTTCTTGAACTTAATGGCATTTGCGTC-TTACTAT-CCATTAAATG 751 2 463 .......................................................................-.......-.......... 550 0 627 .......................................................................T.......C.......... 716 E K F V K E K G N K F G L E S D T T L Y N G P F V L T D W K Query_1 752 AAAAGTTTGTGAAAGAAAAAGGGAATAAATTCGGTTTAGAGTCTGATACAACACTTTATAATGGACCATTCGTGCTTACTGATTGGAAGC 841 2 551 .......................................................................................... 640 0 717 ......... 725 H E Q G W K L K K N E Q Y W D K K T V K L E E I N Y S V V K Query_1 842 ATGAGCAAGGTTGGAAATTAAAGAAAAATGAGCAGTATTGGGACAAAAAGACTGTCAAACTAGAAGAAATCAATTATAGTGTAGTAAAAG 931 2 641 .......................................................................................... 730 E P A T R V N L Y D T G A I D F T L L S G E F V D K Y R N N Query_1 932 AACCAGCTACTAGAGTAAATTTATATGACACAGGTGCGATTGATTTCACACTTTTATCAGGTGAATTTGTTGATAAGTATAGAAATAATA 1021 2 731 .......................................................................................... 820 K E E F G A Y S E T S T F Y L R L N Q K R G G Q D T P L K S Query_1 1022 AAGAAGAATTTGGTGCATATTCGGAAACAA-GTACGTTTTATTTACGTCTAAACCAAAAACGTGGTGGGCAAGATACACCGTTAAAGAGC 1110 2 821 ..............................-........................................................... 909 1 929 .....A........A............N-.......C.A.....N................N.....N...... 857 K K L R E A I A L S I D K K A L T N V I L N D G S K P V D Query_1 1111 AAAAAACTACGTGAAGCAATTGCATTATCAATTGATAAAAAAGCTTTAACGAA-TGTTATTTT-AAATGATGGTTCAAAACCAGTGGATT 1198 2 910 .....................................................-.........-.......................... 997 2 947 .............. 934 1 856 ......A.T........................................N...A.........T.......................... 767 1 819 .............. 832 Y L V P K G L A S G P D G K D F A E T F K N G L K Q D S K K Query_1 1199 ATTTAGTACCAAAAGGTTTAGCGAGTGGACCAGACGGTAAAGATTTCGCAGAAACGTTCAAAAA-TGGTTTAAAACAAGACTCCAAAAAG 1287 2 998 ................................................................-......................... 1086 1 766 ............................N...................................A......................... 677 A A A A W E E A K K E L G K D Q V T I E L L N Y D T G N A K Query_1 1288 GCAGCGGCAGCCTGGGAAGAAGCGAAAAAAGAACTTGGAAAAGATCAAGTCACAATTGAACTGTTAAACTATGATACTGGTAATGCGAAA 1377 2 1087 .......................................................................................... 1176 2 1442 ........ 1449 1 676 .......................................................................................... 587 1 321 ........ 314 K V G E Y V K D Q V E K N L K G V T V N I K L Q P F K Q K Query_1 1378 AAA-GTTGGGGAGTATGTAAAAGACCAAGTTGAAAAGAATTTAAAAGGTGTAACAGTAAATATTAAACTGCAGCCATTTAAGCAAAAACT 1466 2 1177 ...-...................................................................................... 1265 2 1450 ...C..... 1458 1 586 ...-...................................................................................... 498 1 313 ...C..... 305 L K L E S D Q D Y D F S Y G G W N P D Y A D P M T Y L D M F Query_1 1467 AAAATTAGAATCAGACCAAGATTATGATTTCTCATATGGCGGCTGGAATCCAGATTATGCGGATCCAATGACATACCTTGATATGTTTGA 1556 2 1266 .......................................................................................... 1355 1 497 .......................................................................................... 408 0 45 . 45 E T K N S Q N Q M S Y S N S K Y D D I I T K S K T E W M A D Query_1 1557 AACAAAAAATTCGCAGAACCAGATGAGCTACTCAAATTCAAAATATGATGACATTATTACTAAAAGTAAGACAGAATGGATGGCTGATGC 1646 2 1356 .......................................................................................... 1445 2 1169 .... 1172 1 407 .......................................................................................... 318 1 594 .... 591 0 44 .......... 35 A K K R W T E L G K A E K L L L E E D V A L V P L Y Q T A R Query_1 1647 GAAAAAACGTTGGACAGAGTTAGGGAAAGCAGAAAAATTGTTACTTGAAGAAGATGTAGCGCTTGTGCCTTTATATCAAACTGCTAGATC 1736 2 1446 .......................................................................................... 1535 2 1173 .......-..... 1184 1 317 .......................................................................................... 228 1 590 .......-..... 579 S Y V M K P N I K G I V K H N I S P E Y S F K W A Y V E E K Query_1 1737 ATATGTTATGAAACCAAATATAAAGGGAATTGTGAAACATAATATTAGTCCGGAATATAGCTTTAAATGGGCGTATGTAGAAGAGAAATA 1826 2 1536 ........................................................................................ 1623 1 227 .......................................................................................... 138 * H W K W I T D P N S S S V D K L A A A L E H H H H H H * D Query_1 1827 ACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAG-ATC 1915 1 137 ......................................................................................N... 48 P A A N K Query_1 1916 CGGCTGCTAACAAA 1929 1 47 .......N...... 34 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 6500772 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 3,279 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP05600.102 (IDP05600.20-562.P(562)..pMCSG68)
ACSTSGGDKKTSTNSSSGGDSKSEEKLAAKQVFNKTENQEIPTMDTSKSTDTLGSQILGNTMEGLYRLDKENKPIPAAAESSTKSEDGKKYTFKLRKDAKWSNGDSVTAKDFVFAWQRLLDPKTAAEYAFIAFPIKNAEAVNKGEKPVTELGVKAVDDLTLEVELEQAVPYFLNLVAFPSYYPLNEKFVKEKGDKYGLESDTTVYNGPFVLTDWKHEQGWKLKKNDQYWDKKTVKLDEINYSVVKEPATNVNLYDSGQIDFSLLTGEFVDKYRNNKEEYGVYNEPSTFFIRLNQKRGGQDTPLKSKKLREAIALSIDKKNLTNVILNDGSKPADYLVPKGLAAGPDGKDFQETFKNGVKPDAKKAAAAWEEAKKELGKDQVTIEFLNYDTGNAKKVGEYVKDQIEKNLKGVTVNIKLQPFKQKLKLESDQDYDISYGGWSPDYADPMTYLDMFESKHSHNQMSFSDQKYDEIIKKAGGELMTDAKKRWEELGKAEELLLEEDVALVPLYQSARSYVMKPHVKGVVKHNISPEYSYKWAYVTEK
GCATGTTCTACATCAGGTGGAGATAAGAAGACAAGCACAAATTCTAGCTCTGGCGGAGATAGCAAATCAGAAGAAAAATTAGCAGCGAAACAAGTGTTCAATAAAACAGAAAATCAGGAAATTCCAACAATGGATACGTCGAAGAGTACAGATACTTTGGGCTCTCAAATTTTAGGGAACACAATGGAAGGCTTATACCGTTTAGATAAAGAGAATAAACCAATCCCAGCTGCGGCAGAATCAAGCACGAAGAGTGAGGATGGTAAAAAATATACATTTAAATTACGTAAAGATGCAAAATGGTCAAATGGTGATTCTGTAACAGCGAAAGATTTCGTATTTGCATGGCAACGTTTGTTAGATCCAAAAACAGCTGCTGAGTATGCATTCATTGCATTCCCAATTAAAAATGCAGAAGCGGTTAATAAAGGGGAAAAACCTGTAACAGAACTAGGAGTTAAAGCAGTAGATGACCTTACTTTAGAAGTTGAATTAGAACAAGCAGTACCATACTTCTTAAACTTAGTAGCATTCCCATCATACTATCCATTAAATGAGAAATTCGTAAAAGAAAAAGGGGATAAATACGGTTTAGAATCTGATACAACTGTATATAACGGACCGTTCGTTCTTACTGATTGGAAGCATGAGCAAGGCTGGAAACTGAAGAAGAATGATCAATATTGGGATAAAAAGACTGTAAAATTAGATGAAATTAACTATAGTGTTGTAAAAGAGCCAGCAACTAATGTGAATTTATATGATAGTGGTCAAATTGATTTCTCCTTATTAACAGGTGAATTTGTTGACAAATATAGAAATAATAAAGAGGAATATGGGGTGTATAACGAACCAAGTACGTTCTTTATACGTTTAAACCAAAAACGTGGTGGACAAGATACACCATTAAAGAGTAAAAAATTACGTGAAGCAATTGCGCTATCAATTGATAAAAAGAATTTAACAAATGTAATTTTAAATGATGGGTCAAAACCTGCAGATTATTTAGTGCCGAAAGGATTAGCAGCTGGACCTGATGGAAAAGACTTCCAAGAAACGTTTAAAAACGGTGTTAAACCAGATGCGAAAAAAGCAGCTGCTGCATGGGAAGAAGCGAAAAAAGAACTTGGAAAAGATCAAGTTACAATTGAGTTTTTAAACTATGATACTGGTAATGCGAAAAAAGTTGGGGAATATGTAAAAGATCAAATTGAGAAAAACTTAAAAGGTGTAACAGTAAATATTAAACTGCAACCGTTTAAACAAAAACTTAAATTAGAATCAGATCAAGATTATGATATTTCATATGGTGGATGGAGCCCAGATTATGCGGATCCAATGACATATTTAGATATGTTTGAAAGTAAGCACTCTCATAACCAAATGAGTTTCTCTGATCAGAAATATGATGAAATAATCAAAAAAGCTGGTGGAGAATTAATGACTGATGCGAAGAAACGCTGGGAGGAGCTAGGAAAAGCAGAGGAATTACTTCTTGAAGAAGATGTTGCCCTTGTACCTTTATATCAAAGTGCTAGATCTTATGTTATGAAACCGCATGTAAAAGGTGTTGTTAAGCATAATATTAGCCCAGAATACAGCTATAAGTGGGCATATGTTACTGAAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,866 total letters Query= IDP05600.102 Length=2034 Score E Sequences producing significant alignments: (Bits) Value wt 2938 0.0 01F_F1_IDP05600.102_T7short.seq 1896 0.0 07F_F1_IDP05600.102_T7term.seq 1654 0.0 Q F P S R N N F V * L * E G D I H M H H H H H H S S G V D Query_1 55 CAATTCCCC--TCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATC 142 2 678 ........... 668 0 13 .........CT............................................................................... 102 0 840 ........... 830 L W S H P Q F E K G T E N L Y F Q S N A A C S T S G G D K K Query_1 143 TGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGCATGTTCTACATCAGGTGGAGATAAGAAGA 232 2 1 ............................... 31 0 103 .......................................................................................... 192 T S T N S S S G G D S K S E E K L A A K Q V F N K T E N Q E Query_1 233 CAAGCACAAATTCTAGCTCTGGCGGAGATAGCAAATCAGAAGAAAAATTAGCAGCGAAACAAGTGTTCAATAAAACAGAAAATCAGGAAA 322 2 32 .......................................................................................... 121 0 193 .......................................................................................... 282 I P T M D T S K S T D T L G S Q I L G N T M E G L Y R L D K Query_1 323 TTCCAACAATGGATACGTCGAAGAGTACAGATACTTTGGGCTCTCAAATTTTAGGGAACACAATGGAAGGCTTATACCGTTTAGATAAAG 412 2 122 .......................................................................................... 211 0 283 .......................................................................................... 372 E N K P I P A A A E S S T K S E D G K K Y T F K L R K D A K Query_1 413 AGAATAAACCAATCCCAGCTGCGGCAGAATCAAGCACGAAGAGTGAGGATGGTAAAAAATATACATTTAAATTACGTAAAGATGCAAAAT 502 2 212 .......................................................................................... 301 0 373 .......................................................................................... 462 W S N G D S V T A K D F V F A W Q R L L D P K T A A E Y A F Query_1 503 GGTCAAATGGTGATTCTGTAACAGCGAAAGATTTCGTATTTGCATGGCAACGTTTGTTAGATCCAAAAACAGCTGCTGAGTATGCATTCA 592 2 302 .......................................................................................... 391 0 463 .......................................................................................... 552 I A F P I K N A E A V N K G E K P V T E L G V K A V D D L T Query_1 593 TTGCATTCCCAATTAAAAATGCAGAAGCGGTTAATAAAGGGGAAAAACCTGTAACAGAACTAGGAGTTAAAGCAGTAGATGACCTTACTT 682 2 392 .......................................................................................... 481 0 553 .......................................................................................... 642 L E V E L E Q A V P Y F L N L V A F P S Y Y P L N E K F V K Query_1 683 TAGAAGTTGAATTAGAACAAGCAGTACCATACTTCTT-AAACTTAGTAGCATTCCCATCATACTATCCATTAAATGAGAAATTCGTAAAA 771 2 482 .....................................-.................................................... 570 0 643 .....................................A.................................................... 732 E K G D K Y G L E S D T T V Y N G P F V L T D W K H E Q G W Query_1 772 GAAAAAGGGGATAAATACGGTTTAGAATCTGATACAACTGTATATAACGGACCGTTCGTTCTTACTGATTGGAAGCATGAGCAAGGCTGG 861 2 571 .......................................................................................... 660 0 733 .......................................................................................... 822 K L K K N D Q Y W D K K T V K L D E I N Y S V V K E P A T N Query_1 862 AAACTGAAGAAGAATGATCAATATTGGGATAAAAAGACTGTAAAATTAGATGAAATTAACTATAGTGTTGTAAAAGAGCCAGCAACTAAT 951 2 661 .......................................................................................... 750 0 823 ....................................................-..................................... 911 0 92 ........... 82 V N L Y D S G Q I D F S L L T G E F V D K Y R N N K E E Y Query_1 952 GTGAATTTATATGATAGTGGTCAAATTGATTTCTCCTTATTAAC-AGGTGAATTTGTTG-ACAAATATAGAAATAATAAAGAGGAATATG 1039 2 751 ............................................-..............-.............................. 838 0 912 ............................................-..............-..-.......C................... 998 1 994 ........................N......GN.N.N.KN...............-........G..N.. 926 G V Y N E P S T F F I R L N Q K R G G Q D T P L K S K K Query_1 1040 GGGTGTATAACGAACCAAGTACGTTC-TTTATACG-TTTAAACCAAAAACGTGGTGG-ACAA-GATACACCATTAAAGAG-TAAAAAATT 1124 2 839 ..........................-........-.....................-....-.................-......... 923 0 999 ........-.....A.C.........T...T....T..................N..A....-.....-N.N........T......... 1085 1 925 .............C............-........-.....C......-........-....A.............-...-........- 843 L R E A I A L S I D K K N L T N V I L N D G S K P A D Y L V Query_1 1125 ACGTGAAGCAATT-GCGCTATCAATTGATAAAAAGAATTTAA-CAAATGTAATTTTAAATGATGGGTCAAAACCTGCAGATTATTTAGTG 1212 2 924 .............-............................-............................................... 1011 2 952 ............ 941 0 1086 .............G.....N..-....-NN...........N-NN...........-..N.......... 1151 1 842 .............-............................A............................................... 754 1 814 ............ 825 P K G L A A G P D G K D F Q E T F K N G V K P D A K K A A Query_1 1213 CCGAAAGGATTAGCAGCTGGACCTGATGGAAAAGACTT-CCAAGAAACGTTTAAAAACGGTGTTAAACCAGATGCGAAAAAAGCAGCTGC 1301 2 1012 ......................................-................................................... 1100 2 1175 ............ 1186 1 753 ......................................T................................................... 664 1 589 ............ 578 A A W E E A K K E L G K D Q V T I E F L N Y D T G N A K K V Query_1 1302 TGCATGGGAAGAAGCGAAAAAAGAACTTGGAAAAGATCAAGTTACAATTGAGTTTTTAAACTATGATACTGGTAATGCGAAAAAAGTTGG 1391 2 1101 .......................................................................................... 1190 2 1082 ............ 1093 1 663 .......................................................................................... 574 1 682 ............ 671 G E Y V K D Q I E K N L K G V T V N I K L Q P F K Q K L K L Query_1 1392 GGAATATGTAAAAGATCAAATTGAGAAAAACTTAAAAGGTGTAACAGTAAATATTAAACTGCAACCGTTTAAACAAAAACTTAAATTAGA 1481 2 1191 .......................................................................................... 1280 1 573 .......................................................................................... 484 E S D Q D Y D I S Y G G W S P D Y A D P M T Y L D M F E S K Query_1 1482 ATCAGATCAAGATTATGATATTTCATATGGTGGATGGAGCCCAGATTATGCGGATCCAATGACATATTTAGATATGTTTGAAAGTAAGCA 1571 2 1281 .......................................................................................... 1370 1 483 .......................................................................................... 394 H S H N Q M S F S D Q K Y D E I I K K A G G E L M T D A K K Query_1 1572 CTCTCATAACCAAATGAGTTTCTCTGATCAGAAATATGATGAAATAATCAAAAAAGCTGGTGGAGAATTAATGACTGATGCGAAGAAACG 1661 2 1371 .......................................................................................... 1460 1 393 .......................................................................................... 304 R W E E L G K A E E L L L E E D V A L V P L Y Q S A R S Y V Query_1 1662 CTGGGAGGAGCTAGGAAAAGCAGAGGAATTACTTCTTGAAGAAGATGTTGCCCTTGTACCTTTATATCAAAGTGCTAGATCTTATGTTAT 1751 2 1461 .......................................................................................... 1550 1 303 .......................................................................................... 214 M K P H V K G V V K H N I S P E Y S Y K W A Y V T E K * H W Query_1 1752 GAAACCGCATGTAAAAGGTGTTGTTAAGCATAATATTAGCCCAGAATACAGCTATAAGTGGGCATATGTTACTGAAAAATAACATTGGAA 1841 2 1551 ............................................................................... 1629 1 213 .......................................................................................... 124 K W I T D P N S S S V D K L A A A L E H H H H H H * D P A A Query_1 1842 GTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAA 1931 1 123 .......................................................................................... 34 N K A R Query_1 1932 CAAAGCCCGAAA 1943 1 33 ............ 22 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 7694855 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 3,866 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP05634.102 (IDP05634.1-270.F..pMCSG68)
MRRRDMIKRNIIKCLVAVLSLTFVITGCSKKEEVKNEKVITVASSNSADPYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMSKERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVATSVNKDNLFQEKVYGDGTSGLQDLDLGRVSAWIDAELTIVGDAKKANMKVRVLDKKLSATNIAYFFKKNDDKSKTIKEDVNKAIEEMLADGTVKKISEKWFGMDVSADIQK
GTGAGGAGAAGAGATATGATAAAAAGAAATATTATAAAATGTTTAGTGGCAGTTTTATCGTTAACGTTTGTAATTACTGGTTGTTCAAAAAAAGAGGAAGTAAAGAATGAAAAAGTTATAACTGTAGCTTCATCTAATTCAGCTGACCCATATTCTTATGTTGATAATGGTGAGCATAAGGGTTTTGAAGTTGACATGTGGAAAGAGATTGGTAAAAGAAGTGGATATAAGATAAAAATGGAACCAACAGGTTTTAGTTCTATATTTGGTATGTTAGATTCTGGTAAAGTTGATGTGGCAGCCAACTTCTTTGGAATGTCTAAAGAAAGATTAGAAAAATACGATGCTTCTATACCTTATGGTTCTGATTCTGTGTGTATAGCTGTAAAAGATGGAAATAACGAAATTAATAAGCTTGAAGATTTAAAGGGAAAAGTTGTAGCAGTTTCAGAAGGTTCTCAAGGTCAACAGGTTGCAACTAGTGTTAATAAAGATAATTTGTTTCAAGAAAAGGTTTACGGTGATGGAACTAGTGGTCTTCAAGATTTAGATTTGGGAAGAGTTAGTGCATGGATTGATGCAGAGCTTACTATTGTGGGAGACGCTAAAAAAGCTAATATGAAAGTAAGAGTATTAGATAAAAAATTATCAGCTACTAATATAGCATACTTCTTTAAGAAAAACGATGATAAATCTAAAACGATAAAAGAAGATGTAAACAAAGCAATAGAAGAAATGCTTGCAGATGGAACAGTGAAAAAAATATCTGAAAAATGGTTTGGAATGGATGTAAGTGCTGATATACAAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,908 total letters Query= IDP05634.102 Length=1215 Score E Sequences producing significant alignments: (Bits) Value wt 1462 0.0 12A_A6_IDP05634.102_T7term.seq 1456 0.0 06A_A6_IDP05634.102_T7short.seq 1416 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * Query_1 1 TCTCGATCCC-GCGAAATT-AATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCC-TCTAGAAATAATTTT-GTTTAAC- 85 2 402 .......-.... 412 1 993 ..........C........N......................N......................-...............-.......- 907 1 924 ...G...........T.T.C.C.C...N......-.A... 962 1 481 .......-.... 471 0 10 ...T..C..N......C....N..........T.......T 50 L * E G D I H M H H H H H H S S G V D L W S H P Q F E K G T Query_1 86 TTTAAG-AAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACC 174 1 906 ..N.GT-......G.TN.G..... 884 0 51 ......T......G.-..G..... 73 E N L Y F Q S N A V R R R D M I K R N I I K C L V A V L S Query_1 175 GAGAACCTGTACTTCCAATCCAATGCCGTGAGGAGAAGAGATATGATAAAAA-GAAATATTATAAAATGTTTAGTGGCAGTTTTATCGTT 263 2 1 .........................-..................................... 62 1 883 .........................A..................................... 821 0 74 .........................-..................................... 135 L T F V I T G C S K K E E V K N E K V I T V A S S N S A D P Query_1 264 AACGTTTGTAATTACTGGTTGTTCaaaaaaaGAGGAAGTAAAGAATGAAAAAGTTATAACTGTAGCTTCATCTAATTCAGCTGACCCATA 353 2 63 .......................................................................................... 152 1 820 .......................................................................................... 731 0 136 .......................................................................................... 225 Y S Y V D N G E H K G F E V D M W K E I G K R S G Y K I K M Query_1 354 TTCTTATGTTGATAATGGTGAGCATAAGGGTTTTGAAGTTGACATGTGGAAAGAGATTGGTAAAAGAAGTGGATATAAGATAAAAATGGA 443 2 153 .......................................................................................... 242 1 730 .......................................................................................... 641 0 226 .......................................................................................... 315 E P T G F S S I F G M L D S G K V D V A A N F F G M S K E R Query_1 444 ACCAACAGGTTTTAGTTCTATATTTGGTATGTTAGATTCTGGTAAAGTTGATGTGGCAGCCAACTTCTTTGGAATGTCTAAAGAAAGATT 533 2 243 .......................................................................................... 332 1 640 .......................................................................................... 551 0 316 .......................................................................................... 405 L E K Y D A S I P Y G S D S V C I A V K D G N N E I N K L E Query_1 534 AGAAAAATACGATGCTTCTATACCTTATGGTTCTGATTCTGTGTGTATAGCTGTAAAAGATGGAAATAACGAAATTAATAAGCTTGAAGA 623 2 333 .......................................................................................... 422 1 550 .......................................................................................... 461 0 406 ......................................................................N................... 495 D L K G K V V A V S E G S Q G Q Q V A T S V N K D N L F Q E Query_1 624 TTTAAAGGGAAAAGTTGTAGCAGTTTCAGAAGGTTCTCAAGGTCAACAGGTTGCAACTAGTGTTAATAAAGATAATTTGTTTCAAGAAAA 713 2 423 .......................................................................................... 512 1 460 .......................................................................................... 371 0 496 .......................................................................................... 585 K V Y G D G T S G L Q D L D L G R V S A W I D A E L T I V G Query_1 714 GGTTTACGGTGATGGAACTAGTGGTCTTCAAGATTTAGATTTGGGAAGAGTTAGTGCATGGATTGATGCAGAGCTTACTATTGTGGGAGA 803 2 513 .......................................................................................... 602 1 370 .......................................................................................... 281 0 586 .......................................................................................... 675 D A K K A N M K V R V L D K K L S A T N I A Y F F K K N D D Query_1 804 CGCTAAAAAAGCTAATATGAAAGTAAGAGTATTAGATAAAAAATTATCAGCTACTAATATAGCATACTTC-TTTAAGAAAAACGATGATA 892 2 603 ......................................................................-................... 691 1 280 ......................................................................-................... 192 0 676 ................................................N.....................T................... 765 K S K T I K E D V N K A I E E M L A D G T V K K I S E K W Query_1 893 AATCT-AAAACGATAAAAGAAGATGTAAACAAAGCAATAGAAGAAATGCTTGCAGATGGAACAGTGaaaaaaaTATCTG-AAAAATGGTT 980 2 692 .....-.........................................................................-.......... 779 1 191 .....-.........................................................................-.......... 104 0 766 .....A...................N.....................................................A.......... 855 F G M D V S A D I Q K * H W K W I T D P N S S S V D K L A A Query_1 981 TGGAATGGATGTAAGTGC-TGATATACAAAAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCG 1069 2 780 ..................-............. 810 1 103 ..................-.............C.T... 67 0 856 .T............N...N............. 887 A L E H H H H H H * D P A A N K A R K E A E L A A A Query_1 1070 CACTCGAGcaccaccaccaccaccacTGAG-A-TCC-GGCTGCT-AAC--AAAGCCC--GAAAGGAAGC-TGAGTTGGCTG--CTGCCAC 1148 1 72 ..T..T.....T.....T.A..-.A...-.......-...AA.......CGA....... 17 0 891 ..T.....T....CT.T..G.-...C.......T...--.......C-..C.......CN..A......TGC.....N. 965 T A E Q * L A * P L G A S K R V L R G F L L K Query_1 1149 CG-CTGAGCAATAACTAGCATAACCCCTTGGGGCCT-CTAAACGGGTCTTGAGGGGTTTTTT-GCTGAAA 1215 0 966 ..N.............NN..NT..............N........ANN.N............G....... 1035 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3429140 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 2,908 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP05634.105 (IDP05634.31-270.P(270)..pMCSG73)
KEEVKNEKVITVASSNSADPYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMSKERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVATSVNKDNLFQEKVYGDGTSGLQDLDLGRVSAWIDAELTIVGDAKKANMKVRVLDKKLSATNIAYFFKKNDDKSKTIKEDVNKAIEEMLADGTVKKISEKWFGMDVSADIQK
AAAGAGGAAGTAAAGAATGAAAAAGTTATAACTGTAGCTTCATCTAATTCAGCTGACCCATATTCTTATGTTGATAATGGTGAGCATAAGGGTTTTGAAGTTGACATGTGGAAAGAGATTGGTAAAAGAAGTGGATATAAGATAAAAATGGAACCAACAGGTTTTAGTTCTATATTTGGTATGTTAGATTCTGGTAAAGTTGATGTGGCAGCCAACTTCTTTGGAATGTCTAAAGAAAGATTAGAAAAATACGATGCTTCTATACCTTATGGTTCTGATTCTGTGTGTATAGCTGTAAAAGATGGAAATAACGAAATTAATAAGCTTGAAGATTTAAAGGGAAAAGTTGTAGCAGTTTCAGAAGGTTCTCAAGGTCAACAGGTTGCAACTAGTGTTAATAAAGATAATTTGTTTCAAGAAAAGGTTTACGGTGATGGAACTAGTGGTCTTCAAGATTTAGATTTGGGAAGAGTTAGTGCATGGATTGATGCAGAGCTTACTATTGTGGGAGACGCTAAAAAAGCTAATATGAAAGTAAGAGTATTAGATAAAAAATTATCAGCTACTAATATAGCATACTTCTTTAAGAAAAACGATGATAAATCTAAAACGATAAAAGAAGATGTAAACAAAGCAATAGAAGAAATGCTTGCAGATGGAACAGTGAAAAAAATATCTGAAAAATGGTTTGGAATGGATGTAAGTGCTGATATACAAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,150 total letters Query= IDP05634.105 Length=1125 Score E Sequences producing significant alignments: (Bits) Value wt 1299 0.0 12F_F6_IDP05634.105_T7term.seq 1141 0.0 06F_F6_IDP05634.105_T7short.seq 60.8 1e-12 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAA 90 2 312 ........... 322 1 887 .......N...N...T.--...N.............................................-...N..NN.....N....... 801 1 822 ...G...........T.T.C.C.C...........A... 860 1 472 .......... 463 0 11 ..A.........G..--..................... 46 E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E Query_1 91 G-AAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAA 179 1 800 .N...... 793 0 47 .-..N..A..T..-G....... 66 0 80 ..N.. 84 N L Y F Q S N A K E E V K N E K V I T V A S S N S A D P Y S Query_1 180 CCTGTACTTCCAATCCAATGCCAAAGAGGAAGTAAAGAATGAAAAAGTTATAACTGTAGCTTCATCTAATTCAGCTGACCCATATTCTTA 269 2 1 .................................................................... 68 1 782 .......N....N...N..........................A....................N... 715 0 85 ......... 93 Y V D N G E H K G F E V D M W K E I G K R S G Y K I K M E P Query_1 270 TGTTGATAATGGTGAGCATAAGGGTTTTGAAGTTGACATGTGGAAAGAGATTGGTAAAAGAAGTGGATATAAGATAAAAATGGAACCAAC 359 2 69 .......................................................................................... 158 1 714 ...........N.........N.....-...........................N............................-..... 627 T G F S S I F G M L D S G K V D V A A N F F G M S K E R L E Query_1 360 AGGTTTTAGTTCTATATTTGGTATGTTAGATTCTGGTAAAGTTGATGTGGCAGCCAACTTCTTTGGAATGTCTAAAGAAAGATTAGAAAA 449 2 159 .......................................................................................... 248 1 626 ....N..................................................................................... 537 K Y D A S I P Y G S D S V C I A V K D G N N E I N K L E D L Query_1 450 ATACGATGCTTCTATACCTTATGGTTCTGATTCTGTGTGTATAGCTGTAAAAGATGGAAATAACGAAATTAATAAGCTTGAAGATTTAAA 539 2 249 .......................................................................................... 338 1 536 ................................N....N...N.....................N.......................... 447 K G K V V A V S E G S Q G Q Q V A T S V N K D N L F Q E K V Query_1 540 GGGAAAAGTTGTAGCAGTTTCAGAAGGTTCTCAAGGTCAACAGGTTGCAACTAGTGTTAATAAAGATAATTTGTTTCAAGAAAAGGTTTA 629 2 339 .......................................................................................... 428 1 446 .......................N.....N.........G.................................................N 357 Y G D G T S G L Q D L D L G R V S A W I D A E L T I V G D A Query_1 630 CGGTGATGGAACTAGTGGTCTTCAAGATTTAGATTTGGGAAGAGTTAGTGCATGGATTGATGCAGAGCTTACTATTGTGGGAGACGCTAA 719 2 429 .......................................................................................... 518 1 356 ................................................A...........N..............N..A....G...... 267 K K A N M K V R V L D K K L S A T N I A Y F F K K N D D K S Query_1 720 AAAAGCTAATATGAAAGTAAGAGTATTAGATAAAAAATTATCAGCTACTAATATAGCATACTTCTTTAAGAAAAACGATGATAAATCTAA 809 2 519 .......................................................................................... 608 1 266 .....N.....N..................................N........................................... 177 K T I K E D V N K A I E E M L A D G T V K K I S E K W F G M Query_1 810 AACGATAAAAGAAGATGTAAACAAAGCAATAGAAGAAATGCTTGCAGATGGAACAGTGaaaaaaaTATCTGAAAAATGGTTTGGAATGGA 899 2 609 .......................................................................................... 698 1 176 NN..............-A..N...................................G........A........................ 88 D V S A D I Q K * H Query_1 900 TGTAAGTGCTGATATACAAAAATAACAT 927 2 699 ...................... 720 1 87 ........G....-G.......C.T... 61 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2336550 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 2,150 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP05678.102 (IDP05678.20-256.P(256)..pMCSG68)
EDTPEKVQTTTIHISAAASLKDSIDDVKPLFEKANPTIKLSFDFGGSGQIRERVESGAPIDGVLLASKKDADTLIKQNLAEKTKEFAGNELVLIEPKNVDQKTEANLEQLLNDASKIAIGDPESVPAGAYAKQTLENLNLYNAEKAKLVLATDVRQVLSYVEAGNADAGFVYQTDALLSKKVQVKAKIDEKLHDPIAYYSAQVSDSDKKEETATFLDFMNKSEAQKILEKYGFKAAN
GAGGATACGCCTGAGAAAGTCCAAACAACGACTATACATATTTCAGCAGCAGCAAGTTTAAAAGATTCGATAGACGACGTAAAACCACTTTTTGAAAAAGCCAATCCAACGATTAAATTATCTTTTGATTTTGGTGGTTCTGGACAAATTCGCGAACGTGTCGAAAGCGGGGCACCAATTGACGGCGTCCTTTTAGCGAGTAAAAAAGATGCAGATACACTGATTAAGCAAAATTTAGCAGAAAAAACGAAAGAATTTGCGGGAAATGAACTCGTTTTAATTGAACCTAAAAACGTGGATCAGAAGACAGAGGCGAACTTAGAACAATTACTAAATGACGCATCCAAAATCGCCATCGGGGACCCAGAATCAGTACCAGCAGGTGCTTATGCCAAACAAACGCTCGAAAACCTAAATCTATATAATGCAGAAAAAGCAAAGCTTGTTCTTGCAACCGATGTTCGTCAAGTCTTATCCTACGTGGAAGCCGGAAATGCAGATGCAGGCTTCGTTTACCAGACAGATGCTCTATTAAGCAAGAAAGTACAAGTGAAGGCAAAAATCGATGAGAAACTCCATGATCCAATCGCTTACTACAGCGCACAAGTCAGTGATTCAGATAAAAAAGAAGAAACCGCTACATTTCTTGATTTTATGAATAAATCAGAAGCGCAAAAAATACTGGAAAAATACGGATTCAAAGCAGCAAAC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,938 total letters Query= IDP05678.102 Length=1116 Score E Sequences producing significant alignments: (Bits) Value 09G_G3_IDP05678.102_T7term.seq 1750 0.0 03G_G3_IDP05678.102_T7short.seq 1572 0.0 wt 1283 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCT-CTAG-AAATAATTTTGTTT-AAC-- 85 1 1040 ...................-........N...NN...-...N...........-........T.-..N.G.....--.......-...-- 960 0 21 .....N..N.-..TN..........T...CT 50 L * E G D I H M H H H H H H S S G V D L W S H P Q F E K G T Query_1 86 TTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCG 175 1 959 ..-.........................................-............................................. 872 0 51 .......................................................................................... 140 E N L Y F Q S N A E D T P E K V Q T T T I H I S A A A S L K Query_1 176 AGAACCTGTACTTCCAATCCAATGCCGAGGATACGCCTGAGAAAGTCCAAACAACGACTATACATATTTCAGCAGCAGCAAGTTTAAAAG 265 1 871 ...................................................................................N...... 782 0 141 .......................................................................................... 230 2 1 ................................................................ 64 D S I D D V K P L F E K A N P T I K L S F D F G G S G Q I R Query_1 266 ATTCGATAGACGACGTAAAACCACTTTTTGAAAAAGCCAATCCAACGATTAAATTATCTTTTGATTTT-GGTGGTTCTGGACAAATTCGC 354 1 781 ............................-.......................................T..................... 693 0 231 ....................................................................-..................... 319 2 65 ....................................................................-..................... 153 E R V E S G A P I D G V L L A S K K D A D T L I K Q N L A E Query_1 355 GAACGTGTCGAAAGCGGGGCACCAATTGACGGCGTCCTTTTAGCGAGTAAAAAAGATGCAGATACACTGATTAAGCAAAATTTAGCAGAA 444 1 692 .......................................................................................... 603 0 320 .......................................................................................... 409 2 154 .......................................................................................... 243 K T K E F A G N E L V L I E P K N V D Q K T E A N L E Q L L Query_1 445 AAAACGAAAGAATTTGCGGGAAATGAACTCGTTTTAATTGAACCTAAAAACGTGGATCAGAAGACAGAGGCGAACTTAGAACAATTACTA 534 1 602 .......................................................................................... 513 0 410 .......................................................................................... 499 2 244 .......................................................................................... 333 N D A S K I A I G D P E S V P A G A Y A K Q T L E N L N L Y Query_1 535 AATGACGCATCCAAAATCGCCATCGGGGACCCAGAATCAGTACCAGCAGGTGCTTATGCCAAACAAACGCTCGAAAACCTAAATCTATAT 624 1 512 .......................................................................................... 423 0 500 .......................................................................................... 589 2 334 .......................................................................................... 423 N A E K A K L V L A T D V R Q V L S Y V E A G N A D A G F Query_1 625 AATGCAGAAAAAGCAAAGCTTGTTCTTGCAACCGATGTTCGTCAAGTCTTATCCTACGTGGAAGCCGGAAATGCAGATGCAGGCTTCG-T 713 1 422 ........................................................................................-. 334 0 590 ........................................................................................T. 679 2 424 ........................................................................................-. 512 V Y Q T D A L L S K K V Q V K A K I D E K L H D P I A Y Y S Query_1 714 TTACCAGACAGATGCTCTATTAAGCAAGAAAGTACAAGTGAA-GGCAAAAATCGATGAGAAACTCCATGATCCAATCGCTTACTACAGCG 802 1 333 ..........................................-............................................... 245 0 680 ..........................................N............................................... 769 2 513 ..........................................-............................................... 601 A Q V S D S D K K E E T A T F L D F M N K S E A Q K I L E K Query_1 803 CACAAGTCAGTGATTCAGATAAAAAAGAAGAAACCGCTACATTTCTTGATTTTATGAATAAATCAGAAGCGCAAAAAATAC-TGGAAAAA 891 1 244 .................................................................................-........ 156 0 770 ................................N................................................T........ 859 2 602 .................................................................................-........ 690 Y G F K A A N * H W K W I T D P N S S S V D K L A A A L Query_1 892 TAC-GGATTCAAAGCAGCAAACTAACATT-GGAAGTGGATAAC-GGAT-CCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGA 977 1 155 ...-.........................-.............-....-......................................... 70 0 860 ...G.......N...........N..N..G.............G....N......................N.................. 949 2 691 ...-.................. 711 E H H H H H H * D P A A N K A R Query_1 978 -Gca-cca-ccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAA 1025 1 69 -...-...-.......................................... 22 0 950 N...N...C.....NA........ 973 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3179000 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 2,938 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP05684.102 (IDP05684.1-288.F..pMCSG68)
MTKSRFFSDVAETSSFVFAVAGADDEVVLETIRLALKQKLGKFLLFGKKEDKTLTANESVTWIQTDTAEAAAQGAILAVKNKEADILVKGFIPTATLMHHVLKKENGLRTDQLLSQIAIFDIPTYHKPLLITDCAMNVAPKTKEKIAITENALAVAHQIGITNPKIALLSAVEEVTAKMPSTLEAQEVVQHFGNQISVSGPLALDVAISKEAALHKGITDSSAGEADILIAPNIETGNALYKSLVYFAGAKVGSAVVGAKVPIVISSRNDSPENKLASFILTVRLVEK
ATGACAAAAAGCAGATTTTTTTCAGATGTAGCAGAAACGAGTTCATTTGTTTTTGCAGTTGCCGGCGCAGATGATGAGGTGGTATTAGAAACCATTCGTCTTGCATTAAAACAAAAACTAGGTAAATTTCTTTTATTTGGTAAGAAAGAAGACAAAACCTTAACAGCCAACGAAAGTGTTACGTGGATTCAAACTGATACCGCAGAAGCTGCAGCGCAGGGTGCCATTTTAGCTGTAAAAAATAAAGAAGCAGACATTTTAGTAAAAGGATTCATCCCAACAGCTACACTGATGCACCACGTATTAAAAAAAGAAAATGGCCTTCGAACTGACCAATTATTAAGTCAAATTGCTATTTTCGACATTCCGACGTATCACAAGCCATTATTAATAACTGATTGTGCCATGAACGTAGCACCAAAAACAAAGGAAAAAATTGCTATCACAGAAAATGCCTTAGCCGTAGCTCATCAGATTGGTATCACCAACCCTAAAATCGCTTTACTTAGTGCAGTAGAAGAAGTGACTGCAAAAATGCCATCTACATTGGAGGCGCAGGAAGTAGTACAGCATTTTGGAAACCAAATATCCGTCTCAGGTCCGCTAGCTCTCGATGTAGCTATTTCCAAAGAAGCGGCTCTCCATAAAGGAATTACGGATAGTTCAGCGGGAGAAGCAGACATATTAATTGCTCCTAATATTGAAACAGGAAATGCACTTTATAAATCATTAGTCTACTTTGCTGGAGCTAAAGTAGGTAGTGCAGTGGTCGGAGCAAAAGTACCTATTGTTATTTCCTCAAGAAATGATTCACCGGAAAACAAATTAGCTTCATTCATACTAACTGTAAGACTAGTTGAGAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,989 total letters Query= IDP05684.102 Length=1269 Score E Sequences producing significant alignments: (Bits) Value 11E_E5_IDP05684.102_T7term.seq 1819 0.0 wt 1559 0.0 05E_E5_IDP05684.102_T7short.seq 1532 0.0 R E I N T T H Y R G I V S G * Q F P S R N N F V * Query_1 8 CCCGC-GAAATTA-ATA-CGA-CTCACTAT--AGGGGAATTGTG--AGCGGATAACAATTCCCCTCTAGAAA--TAA-TTTTGTTTAACT 86 1 1208 .....A....N..G...G...T...-....NA.......G.NN.GA......AT.NC...N.N..NNN.N..AT...-.N.........C 1121 0 16 ..............--...T............ 45 L * E G D I H M H H H H H H S S G V D L W S H P Q F E K G Query_1 87 TTAAGAAGGAGATATA-CATATGCACCATCATCATC-ATCATTCTTCTGG-TGTAGATCTGTGGTCTCATCCGCAGTTCGAAAA-GGGTA 172 1 1120 ..T.....AGN.....T...................C..........N..G..AN....-.N......................A..... 1032 0 46 ..-.............-...................-N..T.........-.................................-..... 130 T E N L Y F Q S N A M T K S R F F S D V A E T S S F V F A Query_1 173 CCGAGAACCTGTACTTCCAAT-CCAATGCCATGACAAAAAGC-AGAtttttttCAGATGTAGCAGAAACGAGTTCATTTGTTTTTGCAGT 260 1 1031 .....................T......G.............C.........................................-..... 943 2 1 ............-............................................... 59 0 131 .....................-....................-.....................N......................... 218 V A G A D D E V V L E T I R L A L K Q K L G K F L L F G K K Query_1 261 TGCCGGCGCAGATGATGAGGTGGTATTAGAAACCATTCGTCTTGCATTAAAACAAAAA-CTAGGTAAATTTCTTTTATTTGGTAAGAAAG 349 1 942 -..............................-..........................A............................... 855 2 60 ..........................................................-............................... 148 0 219 ..........................................................-............................... 307 E D K T L T A N E S V T W I Q T D T A E A A A Q G A I L A Query_1 350 AA-GACAAAACCTTAACAGCCAACGAAAGTGTTACGTGGATTCAAACTGATACCGCAGAA-GCTGCAGCGCAGGGTGCCATTTTAGCTGT 437 1 854 ..A................................................N........A............................. 765 2 149 ..-.........................................................-............................. 236 0 308 ..-.........................................................-............................. 395 V K N K E A D I L V K G F I P T A T L M H H V L K K E N G L Query_1 438 AAAAAATAAAGAA-GCAGACATTTTAGTAAAAGGATTCATCCCAACAGCTACACTGATGCACC-ACGTATTaaaaaaaGAAAATGGCCTT 525 1 764 .............A.................................................C.......................... 675 1 326 ....-........A... 311 2 237 .............-.................................................-.......................... 324 2 672 ....-........A... 687 0 396 .............-.................................................-.......................... 483 0 835 ....-........A... 850 R T D Q L L S Q I A I F D I P T Y H K P L L I T D C A M N Query_1 526 CGAA-CTGACCAATTATTAAGTCAAATTGCTATTTTCGACATTCCGACGTATCACAAGCCATTATTAATAACTGATTGTGCCATGAACGT 614 1 674 ....A..................................................................................... 585 2 325 ....-..................................................................................... 413 0 484 ....-..................................................................................... 572 V A P K T K E K I A I T E N A L A V A H Q I G I T N P K I A Query_1 615 AGCACCAAAAACAAAGGAAAAAATTGCTATCACAGAAAATGCCTTAGCCGTAGCTCATCAGATTGGTATCACCAACCCTAAAATCGC-TT 703 1 584 .......................................................................................-.. 496 2 414 .......................................................................................-.. 502 0 573 ..................................N....................................................T.. 662 L L S A V E E V T A K M P S T L E A Q E V V Q H F G N Q I S Query_1 704 TACTTAGTGCAGTAGAAGAAGTGACTGCAAAAATGCCATCTACATTGGAGGCGCAGGAAGTAGTACAGCA-TTTTGGAAACCAAATATCC 792 1 495 ......................................................................-................... 407 2 503 ......................................................................-................... 591 0 663 ......................................................................T................... 752 V S G P L A L D V A I S K E A A L H K G I T D S S A G E A Query_1 793 GTCTCAGGTCCGCTAGCTCTCGATGTAGCTA-TTTCCAAAGAAGCGGCTCTCCATAAAGGAATT-ACGGATAGTTCAGCGGGAGAAGCAG 880 1 406 ...............................-................................-......................... 319 2 592 ...............................-................................-......................... 679 2 246 ........ 253 0 753 ..............N............N...T............................N...N........N................ 842 0 405 ........ 412 D I L I A P N I E T G N A L Y K S L V Y F A G A K V G S A V Query_1 881 ACATATTAATTGCTCCTAATATTGAAACAGGAAATGCACTTTATAAATCATTAGTCTAC-TTTGCTGGAGCTAAAGTAGGTAGTGCAGTG 969 1 318 ...........................................................-.............................. 230 2 680 ...........................................................-.............................. 768 2 254 ....T... 261 0 843 ............................................-........N.....T................ 917 0 413 ....T... 420 V G A K V P I V I S S R N D S P E N K L A S F I L T V R L V Query_1 970 GTCGGAGCAAAAGTACCTATTGTTATTTCCTCAAGAAATGATTCACCGGAAAACAAATTAGCTTCATTCATACTAACTGTAAGACTAGTT 1059 1 229 .......................................................................................... 140 2 769 .......................................................................................... 858 E K * H W K W I T D P N S S S V D K L A A A L E H H H H H H Query_1 1060 GAGAAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccac 1149 1 139 ......................................................NNN................................. 50 2 859 ...... 864 * D P A A N K A R K E Query_1 1150 TGAGATCCGGCTGCTAACAAA--GCCCGAAAGGAA 1182 1 49 ............N........AGC........A.. 15 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3685073 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 2,989 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP09018.106 (IDP09018.1-369.F..pMCSG68)
MSQQVTQLNPTQQTTQSAFLATTVITAQCHAILNTQFTPPTVKPDWFDDLSKKLDSAKLVAKQWIDDLGPQVSASIPSSVINFDATFQASIDAIHELYKADPTASGKDNTTVQQASQIMTALSSQVSGIEATVKGMNKELSDWGVKMQAAHDDLVNGATNIQKTIIDLQTDIESMNNAIDNNRAAIEKLNKDLVYAQVAVGVGIFMLVAGVALTVATAGTAAAVSGGIAAVGAASIIAGGVTWGVLQNQIDDDYDSIAQEQKQKAEDQQQIIALQGLSNASSAVVSAIETSTSVLSDFETTWTVFGNELDDVVTKLNNGASMQSIIMEKVMSDAAKNEWDDAVELAKQLASAKIAIETKELAPAVKQAA
ATGTCACAACAAGTTACTCAGTTGAACCCAACACAGCAAACCACTCAATCTGCATTTTTGGCAACCACAGTCATCACAGCGCAGTGCCATGCCATTTTAAACACACAATTTACGCCACCTACGGTAAAACCAGATTGGTTTGATGACCTGAGTAAAAAGTTAGACTCAGCAAAATTGGTTGCTAAGCAATGGATTGACGATTTGGGACCACAAGTATCAGCATCGATTCCCTCCAGCGTAATTAATTTTGACGCTACTTTTCAAGCATCAATCGATGCCATTCATGAGTTATATAAAGCGGATCCAACAGCAAGTGGCAAAGACAATACAACGGTTCAGCAAGCTTCACAGATTATGACCGCCCTATCTTCTCAAGTCAGCGGTATCGAAGCCACAGTCAAAGGGATGAATAAGGAACTCTCTGATTGGGGGGTGAAAATGCAAGCAGCACATGATGACTTAGTCAATGGTGCGACCAATATTCAAAAAACAATTATTGATTTACAAACAGATATTGAAAGTATGAATAACGCAATAGACAACAATCGTGCAGCCATTGAGAAACTGAATAAAGATCTGGTCTATGCACAAGTTGCGGTTGGTGTTGGCATCTTTATGCTCGTCGCAGGCGTTGCGCTTACCGTCGCAACCGCTGGCACCGCAGCTGCAGTCTCCGGCGGTATCGCAGCCGTCGGCGCGGCATCGATTATTGCTGGTGGCGTTACATGGGGCGTATTACAAAATCAAATTGATGATGACTACGACAGCATCGCGCAAGAACAAAAGCAAAAAGCTGAAGATCAACAACAGATTATCGCTTTACAGGGGCTTTCCAATGCCAGTAGCGCCGTTGTTTCAGCGATTGAAACATCGACATCAGTATTATCTGATTTTGAAACAACGTGGACTGTCTTTGGCAACGAGTTAGATGATGTAGTAACAAAGCTTAATAATGGTGCATCCATGCAGAGCATTATAATGGAAAAAGTGATGTCTGACGCAGCGAAAAACGAATGGGATGATGCGGTTGAACTTGCTAAACAGCTAGCTAGCGCAAAAATAGCTATCGAAACGAAAGAGTTAGCACCTGCAGTAAAACAAGCAGCT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 2 sequences; 2,345 total letters Query= IDP09018.106 Length=1512 Score E Sequences producing significant alignments: (Bits) Value wt 1997 0.0 11D_D5_IDP09018.106_T7term.seq 1894 0.0 M S Q Q V T Q L N P T Q Q T T Q S A F L A T T V I T A Q C Query_1 202 ATGTCACAACAAGTTACTCAGTTGAACCCAACACAGCAAACCACTCAATCTGCATTTTTGGCAACCACAGTCA-TCACAGCGCAGTGCCA 290 1 1 .........................................................................-................ 89 0 1170 .......NNN.N......A..NN..GC..NNN.-. 1137 H A I L N T Q F T P P T V K P D W F D D L S K K L D S A K L Query_1 291 TGCCATTTTAAACACACAATTTACGCCACCTACGGTAAAACCAGATTGGTTTGATGACCTGAGTAAAAAGTTAGACTCAGCAAAATTGGT 380 1 90 .......................................................................................... 179 0 1136 ....N...-......C.C.A.......N.-....T--..N........--......N.-............-...........N-..... 1056 V A K Q W I D D L G P Q V S A S I P S S V I N F D A T F Q A Query_1 381 TGCTAAGCAATGGATTGACGATTTGGGACCACAAGTATCAGCATCGATTCCCTCCAGCGTAATTAATTTTGACGCTACTTTTCAAGCATC 470 1 180 .......................................................................................... 269 0 1055 .....-.........-.......................................................N.........-........ 969 S I D A I H E L Y K A D P T A S G K D N T T V Q Q A S Q I M Query_1 471 AATCGATGCCATTCATGAGTTATATAAAGCGGATCCAACAGCAAGTGGCAAAGACAATACAACGGTTCAGCAAGCTTCACAGATTATGAC 560 1 270 .......................................................................................... 359 0 968 .....................................C.................................................... 879 T A L S S Q V S G I E A T V K G M N K E L S D W G V K M Q A Query_1 561 CGCCCTATCTTCTCAAGTCAGCGGTATCGAAGCCACAGTCAAAGGGATGAATAAGGAACTCTCTGATTGGGGGGTGAAAATGCAAGCAGC 650 1 360 .......................................................................................... 449 0 878 ......................................................................................T... 789 A H D D L V N G A T N I Q K T I I D L Q T D I E S M N N A I Query_1 651 ACATGATGACTTAGTCAATGGTGCGACCAATATTCAAAAAACAATTATTGATTTACAAACAGATATTGAAAGTATGAATAACGCAATAGA 740 1 450 .......................................................................................... 539 0 788 .......................................................................................... 699 D N N R A A I E K L N K D L V Y A Q V A V G V G I F M L V A Query_1 741 CAACAATCGTGCAGCCATTGAGAAACTGAATAAAGATCTGGTCTATGCACAAGTTGCGGTTGGTGTTGGCATCTTTATGCTCGTCGCAGG 830 1 540 .......................................................................................... 629 0 698 .......................................................................................... 609 G V A L T V A T A G T A A A V S G G I A A V G A A S I I A G Query_1 831 CGTTGCGCTTACCGTCGCAACCGCTGGCACCGCAGCTGCAGTCTCCGGCGGTATCGCAGCCGTCGGCGCGGCATCGATTATTGCTGGTGG 920 1 630 .......................................................................................... 719 0 608 .......................................................................................... 519 G V T W G V L Q N Q I D D D Y D S I A Q E Q K Q K A E D Q Q Query_1 921 CGTTACATGGGGCGTATTACAAAATCAAATTGATGATGACTACGACAGCATCGCGCAAGAACAAAAGCAAAAAGCTGAAGATCAACAACA 1010 1 720 .......................................................................................... 809 0 518 .......................................................................................... 429 Q I I A L Q G L S N A S S A V V S A I E T S T S V L S D F E Query_1 1011 GATTATCGCTTTACAGGGGCTTTCCAATGCCAGTAGCGCCGTTGTTTCAGCGATTGAAACATCGACATCAGTATTATCTGATTTTGAAAC 1100 1 810 .......................................................................................... 899 0 428 .......................................................................................... 339 T T W T V F G N E L D D V V T K L N N G A S M Q S I I M E K Query_1 1101 AACGTGGACTGTCTTTGGCAACGAGTTAGATGATGTAGTAACAAAGCTTAATAATGGTGCATCCATGCAGAGCATTATAATGGAAAAAGT 1190 1 900 .......................................................................................... 989 0 338 .......................................................................................... 249 V M S D A A K N E W D D A V E L A K Q L A S A K I A I E T K Query_1 1191 GATGTCTGACGCAGCGAAAAACGAATGGGATGATGCGGTTGAACTTGCTAAACAGCTAGCTAGCGCAAAAATAGCTATCGAAACGAAAGA 1280 1 990 .......................................................................................... 1079 0 248 .......................................................................................... 159 E L A P A V K Q A A * H W K W I T D P N S S S V D K L A A A Query_1 1281 GTTAGCACCTGCAGTAAAACAAGCAGCTTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACT 1370 1 1080 ............................ 1107 0 158 .......................................................................................... 69 L E H H H H H H * D P A A N K A R K E Query_1 1371 CGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAA--GGAAG 1426 0 68 ....---............................................AG..... 14 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3460248 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 2,345 Number of sequences in database: 2 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP90634.105 (IDP90634.1-126.F..pMCSG68)
MIRTMLQGKLHRVKVTQADLHYEGSCAIDQDFLEAAGILEYEAIDIYNVDNGQRFSTYAIAAERGSRIISVNGAAARCACVGDKLIICSYVQMSYAAARLHHPKVAYFEGENQLQRKAKAVPVQVA
ATGATACGCACTATGTTGCAAGGCAAACTGCACCGGGTCAAAGTCACTCAAGCGGATTTGCACTATGAAGGCTCCTGCGCCATCGATCAGGATTTTCTGGAAGCCGCCGGTATTCTGGAATACGAAGCTATTGATATTTATAACGTTGATAACGGTCAGCGTTTTTCAACCTATGCGATTGCTGCTGAGCGTGGTTCGCGGATTATCTCCGTGAATGGTGCCGCAGCACGCTGCGCTTGTGTCGGTGATAAGCTCATTATCTGTTCTTATGTACAGATGTCATATGCTGCTGCCCGTTTGCACCACCCTAAAGTGGCTTATTTTGAAGGTGAAAATCAGCTACAACGCAAAGCTAAGGCAGTGCCAGTTCAGGTCGCT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,867 total letters Query= IDP90634.105 Length=783 Score E Sequences producing significant alignments: (Bits) Value 12D_D6_IDP90634.105_T7term.seq 684 0.0 06D_D6_IDP90634.105_T7short.seq 684 0.0 wt 682 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTC-TAGAAATAATTTTGTTTAAC-TTT 88 1 553 .................................................................-....................-... 466 1 486 ...G...........T.T.C.C.C...........A-... 524 0 17 ..T........C.N..................T... 52 * E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E Query_1 89 AAG-AAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAG 177 1 465 ...T......G.-..G..... 446 0 53 ...T......G.-..G..... 72 N L Y F Q S N A M I R T M L Q G K L H R V K V T Q A D L H Y Query_1 178 AACCTGTACTTCCAATCCAATGCCATGATACGCACTATGTTGCAAGGCAAACTGCACCGGGTCAAAGTCACTCAAGCGGATTTGCACTAT 267 1 448 .................................................................. 383 0 70 .................................................................. 135 2 1 .................................................................. 66 E G S C A I D Q D F L E A A G I L E Y E A I D I Y N V D N G Query_1 268 GAAGGCTCCTGCGCCATCGATCAGGATTTTCTGGAAGCCGCCGGTATTCTGGAATACGAAGCTATTGATATTTATAACGTTGATAACGGT 357 1 382 .......................................................................................... 293 0 136 .......................................................................................... 225 2 67 .......................................................................................... 156 Q R F S T Y A I A A E R G S R I I S V N G A A A R C A C V G Query_1 358 CAGCGTTTTTCAACCTATGCGATTGCTGCTGAGCGTGGTTCGCGGATTATCTCCGTGAATGGTGCCGCAGCACGCTGCGCTTGTGTCGGT 447 1 292 .......................................................................................... 203 0 226 .......................................................................................... 315 2 157 .......................................................................................... 246 D K L I I C S Y V Q M S Y A A A R L H H P K V A Y F E G E N Query_1 448 GATAAGCTCATTATCTGTTCTTATGTACAGATGTCATATGCTGCTGCCCGTTTGCACCACCCTAAAGTGGCTTATTTTGAAGGTGAAAAT 537 1 202 .......................................................................................... 113 0 316 .......................................................................................... 405 2 247 .......................................................................................... 336 Q L Q R K A K A V P V Q V A * H W K W I T D P N S S S V D K Query_1 538 CAGCTACAACGCAAAGCTAAGGCAGTGCCAGTTCAGGTCGCTTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAG 627 1 112 ..........................................C.T... 65 0 406 ..........................................C.T... 453 2 337 .......................................... 378 L A A A L E H H H H H H * D P A A N K A R K E A E L A A A Query_1 628 CTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCG--AAAGGAAGCTGAGTTGGCTGCTGCCA 715 1 70 ..T..T.....T.....T.A.......................NGA....... 18 0 448 ..T..T.....T.....T.A........................--.......................... 517 T A E Q * L A * P L G A S K R V L R G F L L K Query_1 716 CCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAA 783 0 518 .................................................................... 585 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2167296 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 2,867 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP90677.102 (IDP90677.1-164.F..pMCSG68)
MNFVSILMGSKSDYETMKEAAKTLESFGVKYELIISSAHRSPKRTKEYIANAEEKGVKVFIAAAGMAAHLAGAVAAYTTKPVLGVPMSGSNLASMDSLFSTVQMPSGIPVGTLAIGKAGAINAAYLAMQILAIYDVDLAQKLKEDRLEKEKKLVSDSKEVEVLL
ATGAATTTTGTTTCTATTTTAATGGGAAGCAAGAGTGATTATGAGACTATGAAAGAGGCTGCTAAAACTTTAGAAAGTTTTGGTGTAAAGTATGAACTTATCATCTCATCAGCTCATCGTAGTCCAAAAAGAACAAAAGAGTATATTGCAAATGCTGAAGAAAAAGGTGTGAAGGTTTTTATAGCAGCAGCTGGAATGGCAGCGCATTTAGCTGGAGCTGTTGCAGCTTATACAACAAAACCTGTTTTAGGAGTGCCTATGTCAGGAAGCAATCTTGCAAGTATGGATTCTTTGTTTTCTACGGTGCAAATGCCTAGTGGAATTCCTGTAGGAACTTTAGCTATTGGTAAGGCTGGAGCAATCAATGCTGCTTATTTGGCTATGCAAATTTTAGCTATTTATGATGTTGATTTAGCACAAAAGCTTAAAGAAGATCGTCTTGAAAAAGAAAAAAAACTTGTATCTGATTCAAAAGAAGTAGAAGTTTTATTA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,000 total letters Query= IDP90677.102 Length=897 Score E Sequences producing significant alignments: (Bits) Value 02D_D2_IDP90677.102_T7short.seq 1501 0.0 08D_D2_IDP90677.102_T7term.seq 1443 0.0 wt 888 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCC--TCTAGAAATAATTTTGTTTAACTTT 88 0 14 .........CT...N...-................. 48 1 828 ...............................................................--......................... 741 1 761 ...G...........T.T.C.C.C..........--.A... 799 * E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E Query_1 89 AAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGA 178 0 49 .......................................................................................... 138 1 740 .......................................................................................... 651 N L Y F Q S N A M N F V S I L M G S K S D Y E T M K E A A K Query_1 179 ACCTGTACTTCCAATCCAATGCCATGAATTTTGTTTCTATTTTAATGGGAAGCAAGAGTGATTATGAGACTATGAAAGAGGCTGCTAAAA 268 0 139 .......................................................................................... 228 1 650 .......................................................................................... 561 2 1 ................................................................... 67 T L E S F G V K Y E L I I S S A H R S P K R T K E Y I A N A Query_1 269 CTTTAGAAAGTTTTGGTGTAAAGTATGAACTTATCATCTCATCAGCTCATCGTAGTCCAAAAAGAACAAAAGAGTATATTGCAAATGCTG 358 0 229 .......................................................................................... 318 1 560 .......................................................................................... 471 2 68 .......................................................................................... 157 E E K G V K V F I A A A G M A A H L A G A V A A Y T T K P V Query_1 359 AAGAAAAAGGTGTGAAGGTTTTTATAGCAGCAGCTGGAATGGCAGCGCATTTAGCTGGAGCTGTTGCAGCTTATACAACAAAACCTGTTT 448 0 319 .......................................................................................... 408 1 470 .......................................................................................... 381 2 158 .......................................................................................... 247 L G V P M S G S N L A S M D S L F S T V Q M P S G I P V G T Query_1 449 TAGGAGTGCCTATGTCAGGAAGCAATCTTGCAAGTATGGATTCTTTGTTTTCTACGGTGCAAATGCCTAGTGGAATTCCTGTAGGAACTT 538 0 409 .......................................................................................... 498 1 380 .......................................................................................... 291 2 248 .......................................................................................... 337 L A I G K A G A I N A A Y L A M Q I L A I Y D V D L A Q K L Query_1 539 TAGCTATTGGTAAGGCTGGAGCAATCAATGCTGCTTATTTGGCTATGCAAATTTTAGCTATTTATGATGTTGATTTAGCACAAAAGCTTA 628 0 499 .......................................................................................... 588 1 290 .......................................................................................... 201 2 338 .......................................................................................... 427 K E D R L E K E K K L V S D S K E V E V L L * H W K W I T D Query_1 629 AAGAAGATCGTCTTGaaaaagaaaaaaaaCTTGTATCTGATTCAAAAGAAGTAGAAGTTTTATTATAACATTGGAAGTGGATAACGGATC 718 0 589 .......................................................................................... 678 1 200 .......................................................................................... 111 2 428 ................................................................. 492 P N S S S V D K L A A A L E H H H H H H * D P A A N K A R Query_1 719 CGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccacc-accacTGAGATCCGGCTGCTAAC-AAAGCCCGAAA 806 0 679 ......................................................-.......................-........... 766 1 110 ......................................................A.......................A........... 21 K E A E L A A A T A E Q * L A * P L G A S K R V L R G F L L Query_1 807 GGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAA 896 0 767 .......................................................................................... 856 1 20 A... 17 K Query_1 897 A 897 0 857 . 857 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2606310 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 3,000 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP90822.102 (IDP90822.1-321.F..pMCSG68)
MHVLIVKTSSMGDVLHTLPALTDAMNAIPGIRFDWVVEEGFSQIPSWHPAVDKVIPVAIRRWRKNWFGSDTRQERCDFKRVVQQRSYDRVIDAQGLIKSAALITRIAKGTKHGPDCKSAREPFASWFYNCRHEIDTKQHAVERIRQLFAKSLGYDKPESYGDYAIAQRFLNQLPADAGQYLVFLHATTRDNKHWPESHWVQLIELVQPTGLKIKLPWGAEHEHQRALRLAEHFPHVEVLPKLSLQQVAEVLAGAKAVVSVDTGLSHLTAALDRPNITLFGPTDPGLIGGYGKNQIAVISEQKKMDSLSAPTIMARLETLIS
ATGCACGTATTAATCGTTAAAACCTCGTCAATGGGCGATGTTTTACATACCTTACCCGCGCTGACGGATGCCATGAATGCCATACCCGGTATTCGCTTCGATTGGGTGGTAGAAGAAGGGTTCAGCCAAATCCCCAGTTGGCATCCGGCGGTGGATAAAGTGATCCCGGTGGCCATCCGCCGTTGGCGTAAAAACTGGTTTGGTAGTGATACCCGGCAGGAACGCTGTGATTTTAAGCGTGTGGTACAACAACGCAGTTATGATCGGGTCATTGATGCCCAAGGGTTGATTAAAAGCGCCGCGCTGATAACCCGGATTGCGAAAGGCACGAAGCACGGGCCGGATTGTAAAAGTGCCCGTGAGCCGTTTGCCAGTTGGTTTTATAACTGCCGCCATGAAATTGATACCAAACAACATGCCGTAGAACGTATACGTCAACTGTTTGCTAAAAGCCTCGGCTATGATAAACCGGAAAGTTATGGCGACTATGCCATCGCACAACGCTTTTTAAATCAGTTACCGGCCGATGCAGGTCAGTACTTGGTTTTCCTACATGCGACGACCCGTGACAACAAGCACTGGCCCGAGAGCCATTGGGTACAATTGATTGAGTTAGTGCAACCCACAGGTTTAAAAATCAAACTACCGTGGGGCGCAGAACATGAGCATCAGCGCGCTTTACGATTAGCGGAACATTTCCCACACGTAGAGGTTCTGCCTAAGCTCAGTCTTCAGCAGGTCGCCGAGGTTCTGGCGGGTGCAAAAGCCGTAGTCTCTGTCGATACTGGCCTCAGCCATCTGACCGCCGCCCTGGATCGCCCAAACATTACGCTGTTTGGCCCCACTGACCCCGGTTTAATCGGCGGCTACGGGAAAAACCAAATTGCCGTGATTTCCGAGCAAAAAAAGATGGATAGCCTCTCTGCGCCCACTATTATGGCACGATTAGAAACACTCATTTCA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,553 total letters Query= IDP90822.102 Length=1368 Score E Sequences producing significant alignments: (Bits) Value wt 1737 0.0 04F_F4_IDP90822.102_T7short.seq 1609 0.0 10F_F4_IDP90822.102_T7term.seq 798 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 60 CCCCTCT-AGAAATAATTTTGTTT-AACTTTAA-GAAGGAGAT-ATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGT 145 0 20 .......T.N..............T........A.........N.............................................. 109 W S H P Q F E K G T E N L Y F Q S N A M H V L I V K T S S M Query_1 146 GGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGCACGTATTAATCGTTAAAACCTCGTCAATGG 235 2 1 .................................. 34 0 110 .......................................................................................... 199 G D V L H T L P A L T D A M N A I P G I R F D W V V E E G F Query_1 236 GCGATGTTTTACATACCTTACCCGCGCTGACGGATGCCATGAATGCCATACCCGGTATTCGCTTCGATTGGGTGGTAGAAGAAGGGTTCA 325 2 35 .......................................................................................... 124 0 200 .......................................................................................... 289 S Q I P S W H P A V D K V I P V A I R R W R K N W F G S D T Query_1 326 GCCAAATCCCCAGTTGGCATCCGGCGGTGGATAAAGTGATCCCGGTGGCCATCCGCCGTTGGCGTAAAAACTGGTTTGGTAGTGATACCC 415 2 125 .......................................................................................... 214 0 290 .......................................................................................... 379 R Q E R C D F K R V V Q Q R S Y D R V I D A Q G L I K S A A Query_1 416 GGCAGGAACGCTGTGATTTTAAGCGTGTGGTACAACAACGCAGTTATGATCGGGTCATTGATGCCCAAGGGTTGATTAAAAGCGCCGCGC 505 2 215 .......................................................................................... 304 0 380 .......................................................................................... 469 L I T R I A K G T K H G P D C K S A R E P F A S W F Y N C R Query_1 506 TGATAACCCGGATTGCGAAAGGCACGAAGCACGGGCCGGATTGTAAAAGTGCCCGTGAGCCGTTTGCCAGTTGGTTTTATAACTGCCGCC 595 2 305 .......................................................................................... 394 0 470 ................N....................................................N.................... 559 H E I D T K Q H A V E R I R Q L F A K S L G Y D K P E S Y G Query_1 596 ATGAAATTGATACCAAACAACATGCCGTAGAACGTATACGTCAACTGTTTGCTAAAAGCCTCGGCTATGATAAACCGGAAAGTTATGGCG 685 2 395 .......................................................................................... 484 0 560 ..............T........................................................................... 649 D Y A I A Q R F L N Q L P A D A G Q Y L V F L H A T T R D N Query_1 686 ACTATGCCATCGCACAACGCTTTTTAAATCAGTTACCGGCCGATGCAGGTCAGTACTTGGTTTTCCTACATGCGACGACCCGTGACAACA 775 2 485 .......................................................................................... 574 0 650 .........................................................................................N 739 K H W P E S H W V Q L I E L V Q P T G L K I K L P W G A E H Query_1 776 AGCACTGGCCCGAGAGCCATTGGGTACAATTGATTGAGTTAGTGC-AACCCACAGGTTTAAAAATCAAACTACCGTGGGGCGCAGAACAT 864 2 575 .............................................-............................................ 663 0 740 .............................................A....T........-..............N.........N..... 828 1 479 ........................-............................................ 412 E H Q R A L R L A E H F P H V E V L P K L S L Q Q V A E V Query_1 865 GAGCATCAGCGCGC-TTTACGATTAGCGGAAC-ATTTCCCACACGTAGAGGTTCTGCCTAAGCTCAGTCTTCAGC-AGGTCGCCGAGGTT 951 2 664 ..............-.................-..........................................-.............. 750 0 829 ..............T.....N...........A..............A...-..............N......NNA....T...T..... 917 1 411 ..............-.................-..........................................-.............. 325 L A G A K A V V S V D T G L S H L T A A L D R P N I T L F Query_1 952 CTGGCG-GGTGCAAAAGCCGTAGTCTCTGTCGATACTGGCCTCAGCCATCTGACCG-CCGCCCTGGATCGCCCAAACATTACG-CTGTTT 1038 2 751 ......-.................................................-..........................-...... 837 0 918 ....G.N.N...........-.A........N........................T.NN......GAN......NTT....NC.G...G 1006 1 324 .N....-.................................................-.....N.............T......-N..... 238 G P T D P G L I G G Y G K N Q I A V I S E Q K K M D S L Query_1 1039 GGCCCCACTGACCCCGGTTTAATCGG-CGGCTACGGG-AAAAACCAAATTGCCG-TGATTTCCGA-GCaaaaaaaGATGGATA--GCCTC 1122 2 838 ..........................-..........-................-..........-.................--..... 921 0 1007 .....NN..N.......G..T.....NN.NN......A...........G....NN.....T...NN..T.....N..N.N..AGC.... 1096 1 237 ..........................-..........-................-..........-.................--..... 154 S A P T I M A R L E T L I S * H W K W I T D P N S S S V D Query_1 1123 TC-TGCGCCCACTATTATGGCACGATTAGAAACACTCATTTCATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAA 1211 2 922 ..-........................................ 963 0 1097 ..T.N..... 1106 1 153 .N-N...................................................................................... 65 K L A A A L E H H H H H H * D P A Query_1 1212 GCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTG-AGAT-CCGGCT 1260 1 64 ...N...................................A....C...... 14 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3386760 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 2,553 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP90994.102 (IDP90994.43-211.P(211)..pMCSG68)
QGNQNTAMTEASSAYQKVSTALSTNSAGNVSADSIALAEKFVQSNKNNYGVLVGLELAHDFVEQNAFDKAAQQLSQALGQTKDENLLSLINLRLARVQLQLKKPDEALKTLDAVQGDGWTAMAQDVRGDALLSKGDTAGARAAYSKAIESNASQALQALLRMKLNNLSS
CAGGGTAATCAGAATACTGCCATGACGGAAGCCTCCTCTGCCTATCAGAAAGTCAGTACTGCTCTGTCAACTAACAGTGCGGGTAATGTTAGTGCTGATAGCATTGCTCTCGCTGAAAAGTTTGTTCAGAGCAATAAAAATAACTACGGCGTACTCGTCGGGTTAGAACTGGCTCATGATTTTGTTGAACAAAATGCATTCGATAAAGCGGCACAGCAACTCAGTCAAGCCTTGGGCCAGACGAAAGATGAAAATCTGTTGTCGCTGATTAATCTGCGCCTTGCGCGTGTGCAACTGCAACTGAAAAAACCCGATGAAGCATTGAAAACGCTGGATGCAGTACAAGGTGATGGCTGGACAGCGATGGCTCAAGATGTTCGTGGCGATGCATTATTAAGCAAAGGTGATACCGCAGGTGCGCGCGCTGCTTATAGTAAAGCTATCGAATCTAATGCTTCTCAGGCGCTGCAAGCATTGTTGCGTATGAAACTGAATAACTTGTCCAGC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,979 total letters Query= IDP90994.102 Length=912 Score E Sequences producing significant alignments: (Bits) Value 10H_H4_IDP90994.102_T7term.seq 1472 0.0 04H_H4_IDP90994.102_T7short.seq 1460 0.0 wt 915 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L Query_1 1 TCTCGATCCCGCGAAA-TTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTT-AACTTT 88 1 842 ................N..................................................................-...... 754 1 774 ...G...........T.T.C.C.C...........A... 812 0 42 ........T...... 56 * E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E Query_1 89 AAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGA 178 1 753 .......................................................................................... 664 0 57 .......................................................................................... 146 N L Y F Q S N A Q G N Q N T A M T E A S S A Y Q K V S T A L Query_1 179 ACCTGTACTTCCAATCCAATGCCCAGGGTAATCAGAATACTGCCATGACGGAAGCCTCCTCTGCCTATCAGAAAGTCAGTACTGCTCTGT 268 1 663 .......................................................................................... 574 1 999 ........ 992 0 147 .......................................................................................... 236 2 1 ................................................................... 67 S T N S A G N V S A D S I A L A E K F V Q S N K N N Y G V L Query_1 269 CAACTAACAGTGCGGGTAATGTTAGTGCTGATAGCATTGCTCTCGCTGAAAAGTTTGTTCAGAGCAATAAAAATAACTACGGCGTACTCG 358 1 573 .......................................................................................... 484 1 991 ... 989 0 237 ......................................................................................N... 326 2 68 .......................................................................................... 157 V G L E L A H D F V E Q N A F D K A A Q Q L S Q A L G Q T K Query_1 359 TCGGGTTAGAACTGGCTCATGATTTTGTTGAACAAAATGCATTCGATAAAGCGGCACAGCAACTCAGTCAAGCCTTGGGCCAGACGAAAG 448 1 483 .......................................................................................... 394 0 327 .......................................................................................... 416 2 158 .......................................................................................... 247 D E N L L S L I N L R L A R V Q L Q L K K P D E A L K T L D Query_1 449 ATGAAAATCTGTTGTCGCTGATTAATCTGCGCCTTGCGCGTGTGCAACTGCAACTGAAAAAACCCGATGAAGCATTGAAAACGCTGGATG 538 1 393 .......................................................................................... 304 0 417 ...........................................................................N.............. 506 2 248 .......................................................................................... 337 A V Q G D G W T A M A Q D V R G D A L L S K G D T A G A R A Query_1 539 CAGTACAAGGTGATGGCTGGACAGCGATGGCTCAAGATGTTCGTGGCGATGCATTATTAAGCAAAGGTGATACCGCAGGTGCGCGCGCTG 628 1 303 .......................................................................................... 214 0 507 .......................................................................................... 596 2 338 .......................................................................................... 427 A Y S K A I E S N A S Q A L Q A L L R M K L N N L S S * H W Query_1 629 CTTATAGTAAAGCTATCGAATCTAATGCTTCTCAGGCGCTGCAAGCATTGTTGCGTATGAAACTGAATAACTTGTCCAGCTAACATTGGA 718 1 213 .......................................................................................... 124 0 597 .......................................................................................... 686 2 428 ................................................................................ 507 K W I T D P N S S S V D K L A A A L E H H H H H H * D P A Query_1 719 AGTGGATAACGGATCCGAATTCGAGCTCC--GTCGACAAGC-TTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTG 805 1 123 .............................--..........-................................................ 37 0 687 .............................CGT.........T................................................ 776 A N K A R K E A E L A A A T A E Q * L A * P L G A S K R V L Query_1 806 CT-AACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTG 894 1 36 ..A...............A... 15 0 777 ..A.......................N.....................................................N......... 866 R G F L L K Query_1 895 AGGGG-TTTTTTGCTGAAA 912 0 867 .....T............. 885 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2631798 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 2,979 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP91025.102 (IDP91025.1-263.F..pMCSG68)
MENNMKNQYGKVSYWVAGAVLLTAFSGPAFAADKITVFAAASLTNALQDIAVQYKQEKQVDVVASYASSSTLARQIEQGAPADLFISADQQWMDYAIDKQQIVANTRYTLLGNELVLIAPQDSQIDKVEIDKKTDWKKLLEGGRLAVGDPDHVPAGIYAKESLENLGAWSTLAPEMARANNVRSAMALVERAEAPLGIVYGSDAVASKKVKVVGIFPEASHKPVEYPMAIVKGHDNPTVTAFYDYLKSPAAAVIFKNYGFTPR
GTGGAGAACAACATGAAGAATCAATACGGTAAAGTGAGTTACTGGGTAGCCGGTGCGGTATTGCTGACGGCTTTTTCCGGCCCAGCCTTCGCTGCCGATAAGATTACCGTGTTTGCCGCGGCCTCACTGACCAATGCGCTACAGGATATTGCTGTCCAGTATAAACAAGAAAAGCAGGTCGATGTGGTGGCTTCCTATGCGTCATCTTCCACGCTGGCACGGCAGATTGAGCAGGGGGCACCGGCCGATCTGTTTATTTCCGCTGACCAACAATGGATGGATTATGCCATTGATAAACAGCAAATTGTCGCCAATACTCGCTACACCCTATTAGGGAACGAACTGGTATTGATTGCGCCGCAAGACAGCCAGATTGATAAAGTTGAGATCGATAAGAAAACCGACTGGAAAAAGTTGCTGGAGGGGGGCCGCTTGGCGGTTGGTGATCCTGACCACGTACCGGCGGGTATTTATGCCAAAGAGTCCCTGGAAAACTTAGGGGCCTGGTCAACGTTAGCCCCGGAAATGGCCCGTGCCAATAATGTGAGAAGCGCAATGGCGCTGGTCGAACGGGCAGAAGCGCCGCTGGGCATTGTTTATGGTTCAGATGCGGTAGCCAGTAAGAAAGTTAAAGTTGTTGGCATATTCCCGGAGGCAAGTCATAAGCCGGTTGAATACCCAATGGCGATTGTTAAAGGCCATGACAATCCAACGGTAACGGCTTTTTATGATTACCTGAAAAGCCCGGCAGCCGCGGTGATTTTCAAAAACTATGGATTCACCCCACGC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,247 total letters Query= IDP91025.102 Length=1194 Score E Sequences producing significant alignments: (Bits) Value 05A_A5_IDP91025.102_T7short.seq 1902 0.0 11A_A5_IDP91025.102_T7term.seq 1900 0.0 wt 1424 0.0 R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L Query_1 3 TCGATCCC--GCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCT-CTAGAAATAATTTTGTTTAACTTTA 89 0 15 ..T.......T......................... 50 1 1120 .....N..CN..............N...N..............G.........-.....T....-...N.....N...-........... 1034 * E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E Query_1 90 AGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTG-TGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGA 178 0 51 .......................................................-.................................. 139 1 1033 ........................................-..............N.................................. 945 N L Y F Q S N A V E N N M K N Q Y G K V S Y W V A G A V L L Query_1 179 ACCTGTACTTCCAATCCAATGCCGTGGAGAACAACATGAA-GAATCAATACGGTAAAGTGAGTTACTGGGTAGCCGGTGCGGTATTGCTG 267 0 140 ........................................-................................................. 228 1 944 ........................................A................................................. 855 2 1 .................-................................................. 66 T A F S G P A F A A D K I T V F A A A S L T N A L Q D I A V Query_1 268 ACGGCTTTTTCCGGCCCAGCCTTCGCTGCCGATAAGATTACCGTGTTTGCCGCGGCCTCACTGACCAATGCGCTACAGGATATTGCTGTC 357 0 229 .......................................................................................... 318 1 854 .......................................................................................... 765 2 67 .......................................................................................... 156 Q Y K Q E K Q V D V V A S Y A S S S T L A R Q I E Q G A P A Query_1 358 CAGTATAAACAAGAAAAGCAGGTCGATGTGGTGGCTTCCTATGCGTCATCTTCCACGCTGGCACGGCAGATTGAGCAGGGGGCACCGGCC 447 0 319 .......................................................................................... 408 1 764 .......................................................................................... 675 2 157 .......................................................................................... 246 D L F I S A D Q Q W M D Y A I D K Q Q I V A N T R Y T L L G Query_1 448 GATCTGTTTATTTCCGCTGACCAACAATGGATGGATTATGCCATTGATAAACAGCAAATTGTCGCCAATACTCGCTACACCCTATTAGGG 537 0 409 .......................................................................................... 498 1 674 .......................................................................................... 585 2 247 .......................................................................................... 336 N E L V L I A P Q D S Q I D K V E I D K K T D W K K L L E G Query_1 538 AACGAACTGGTATTGATTGCGCCGCAAGACAGCCAGATTGATAAAGTTGAGATCGATAAGAAAACCGACTGGAAAAAGTTGCTGGAGGGG 627 0 499 .......................................................................................... 588 1 584 .......................................................................................... 495 2 337 .......................................................................................... 426 G R L A V G D P D H V P A G I Y A K E S L E N L G A W S T L Query_1 628 GGCCGCTTGGCGGTTGGTGATCCTGACCACGTACCGGCGGGTATTTATGCCAAAGAGTCCCTGGAAAACTTAGGGGCCTGGTCAACGTTA 717 0 589 .......................................................................................... 678 1 494 .......................................................................................... 405 2 427 .......................................................................................... 516 A P E M A R A N N V R S A M A L V E R A E A P L G I V Y G S Query_1 718 GCCCCGGAAATGGCCCGTGCCAATAATGTGAGAAGCGCAATGGCGCTGGTCGAACGGGCAGAAGCGCCGCTGGGCATTGTTTATGGTTCA 807 0 679 .......................................................................................... 768 1 404 .......................................................................................... 315 2 517 .......................................................................................... 606 D A V A S K K V K V V G I F P E A S H K P V E Y P M A I V K Query_1 808 GATGCGGTAGCCAGTAAGAAAGTTAAAGTTGTTGGCATATTCCCGGAGGCAAGTCATAAGCCGGTTGAATACCCAATGGCGATTGTTAAA 897 0 769 .......................................................................................... 858 1 314 .......................................................................................... 225 2 607 .......................................................................................... 696 G H D N P T V T A F Y D Y L K S P A A A V I F K N Y G F T P Query_1 898 GGCCATGACAATCCAACGGTAACGGCTTTTTATGATTACCTGAAAAGCCCGGCAGCCGCGGTGATTTTCAAAAACTATGGATTCACCCCA 987 0 859 ...................-N..............................................N...................... 947 1 224 .......................................................................................... 135 2 697 .......................................................................................... 786 R * H W K W I T D P N S S S V D K L A A A L E H H H H H H Query_1 988 CGCTAACATTGGAAG-TGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTT-GCGGCC-GCACTCGAGcaccaccaccaccaccac 1074 0 948 ...............T.......................................G......G.........---............... 1034 1 134 ...............-.......................................-......-.........---............... 51 2 787 ... 789 * D P A A N K A R K E A E L A A A T A E Q * L A * P L G A Query_1 1075 TGAGATCCGGCTGCTAACAAAGCCCG-AAAGGAAGCTGAG-TTGGCTGCTGCCACCGCTG-AGCAATAACTAGCATAACCCCTTGGGGCC 1161 0 1035 ..........................-..N..........T........CTG.NN.....A.NN.............N...T.G...... 1123 1 50 ..........................A....... 17 S K R V L R G F L L K Query_1 1162 TCTAAACGGGTCTT-GAGGGGTTTTTTGCTGAAA 1194 0 1124 ........NNNN..G.NN........GC...... 1157 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3768422 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 3,247 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP91025.103 (IDP91025.32-263.P(263)..pMCSG68)
ADKITVFAAASLTNALQDIAVQYKQEKQVDVVASYASSSTLARQIEQGAPADLFISADQQWMDYAIDKQQIVANTRYTLLGNELVLIAPQDSQIDKVEIDKKTDWKKLLEGGRLAVGDPDHVPAGIYAKESLENLGAWSTLAPEMARANNVRSAMALVERAEAPLGIVYGSDAVASKKVKVVGIFPEASHKPVEYPMAIVKGHDNPTVTAFYDYLKSPAAAVIFKNYGFTPR
GCCGATAAGATTACCGTGTTTGCCGCGGCCTCACTGACCAATGCGCTACAGGATATTGCTGTCCAGTATAAACAAGAAAAGCAGGTCGATGTGGTGGCTTCCTATGCGTCATCTTCCACGCTGGCACGGCAGATTGAGCAGGGGGCACCGGCCGATCTGTTTATTTCCGCTGACCAACAATGGATGGATTATGCCATTGATAAACAGCAAATTGTCGCCAATACTCGCTACACCCTATTAGGGAACGAACTGGTATTGATTGCGCCGCAAGACAGCCAGATTGATAAAGTTGAGATCGATAAGAAAACCGACTGGAAAAAGTTGCTGGAGGGGGGCCGCTTGGCGGTTGGTGATCCTGACCACGTACCGGCGGGTATTTATGCCAAAGAGTCCCTGGAAAACTTAGGGGCCTGGTCAACGTTAGCCCCGGAAATGGCCCGTGCCAATAATGTGAGAAGCGCAATGGCGCTGGTCGAACGGGCAGAAGCGCCGCTGGGCATTGTTTATGGTTCAGATGCGGTAGCCAGTAAGAAAGTTAAAGTTGTTGGCATATTCCCGGAGGCAAGTCATAAGCCGGTTGAATACCCAATGGCGATTGTTAAAGGCCATGACAATCCAACGGTAACGGCTTTTTATGATTACCTGAAAAGCCCGGCAGCCGCGGTGATTTTCAAAAACTATGGATTCACCCCACGC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,187 total letters Query= IDP91025.103 Length=1101 Score E Sequences producing significant alignments: (Bits) Value 11B_B5_IDP91025.103_T7term.seq 1811 0.0 05B_B5_IDP91025.103_T7short.seq 1784 0.0 wt 1256 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAA 90 1 1031 .......................................................................................... 942 1 964 ...G...........T.T.C.C.C...........A... 1002 0 23 ............................... 53 E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E N Query_1 91 GAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAAC 180 1 941 ..............................................................................A........... 852 0 54 .......................................................................................... 143 L Y F Q S N A A D K I T V F A A A S L T N A L Q D I A V Q Y Query_1 181 CTGTACTTCCAATCCAATGCCGCCGATAAGATTACCGTGTTTGCCGCGGCCTCACTGACCAATGCGCTACAGGATATTGCTGTCCAGTAT 270 1 851 .......................................................................................... 762 0 144 .......................................................................................... 233 2 1 ..................................................................... 69 K Q E K Q V D V V A S Y A S S S T L A R Q I E Q G A P A D L Query_1 271 AAACAAGAAAAGCAGGTCGATGTGGTGGCTTCCTATGCGTCATCTTCCACGCTGGCACGGCAGATTGAGCAGGGGGCACCGGCCGATCTG 360 1 761 .......................................................................................... 672 0 234 .......................................................................................... 323 2 70 .......................................................................................... 159 F I S A D Q Q W M D Y A I D K Q Q I V A N T R Y T L L G N E Query_1 361 TTTATTTCCGCTGACCAACAATGGATGGATTATGCCATTGATAAACAGCAAATTGTCGCCAATACTCGCTACACCCTATTAGGGAACGAA 450 1 671 .......................................................................................... 582 0 324 .......................................................................................... 413 2 160 .......................................................................................... 249 L V L I A P Q D S Q I D K V E I D K K T D W K K L L E G G R Query_1 451 CTGGTATTGATTGCGCCGCAAGACAGCCAGATTGATAAAGTTGAGATCGATAAGAAAACCGACTGGAAAAAGTTGCTGGAGGGGGGCCGC 540 1 581 .......................................................................................... 492 0 414 .......................................................................................... 503 2 250 .......................................................................................... 339 L A V G D P D H V P A G I Y A K E S L E N L G A W S T L A P Query_1 541 TTGGCGGTTGGTGATCCTGACCACGTACCGGCGGGTATTTATGCCAAAGAGTCCCTGGAAAACTTAGGGGCCTGGTCAACGTTAGCCCCG 630 1 491 .......................................................................................... 402 0 504 .......................................................................................... 593 2 340 .......................................................................................... 429 E M A R A N N V R S A M A L V E R A E A P L G I V Y G S D Query_1 631 GAAATGGCCCGTGCCAATAATGTGAGAAGCGCAATGGCGCTGGTCGAACGGGCAGAAGCGCCGCTGGGCATT--GTTTATGGTTCAGATG 718 1 401 ........................................................................--................ 314 0 594 ........................................................................TGT............... 683 2 430 ........................................................................--................ 517 A V A S K K V K V V G I F P E A S H K P V E Y P M A I V K G Query_1 719 CGGTAGCCAGTAAGAAAGTTAAAGTTGTTGGCATATTCCCGGAGGCAAGTCATAAGCCGGTTGAATACCCAATGGCGATTGTTAAAGGCC 808 1 313 .......................................................................................... 224 0 684 ...................................................................................-...... 772 2 518 .......................................................................................... 607 H D N P T V T A F Y D Y L K S P A A A V I F K N Y G F T P R Query_1 809 ATGACAATCCAACGGTAACGGCTTTTTATGATTACCTGAAAAGCCCGGCAGCCGCGGTGATTTTCAAAAACTATGGATTCACCCCACGCT 898 1 223 .......................................................................................... 134 0 773 .......................................................................................... 862 2 608 ......................................................................................... 696 * H W K W I T D P N S S S V D K L A A A L E H H H H H H * Query_1 899 AACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGC-TTGCGGCCGCACTCGAGcaccaccaccaccaccacTGA--G 985 1 133 ................................................-......................................AGA 45 0 863 ................................................T......................................--. 950 D P A A N K A R K E A E L A A A T A E Q * L A * P L G A S K Query_1 986 ATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAAC 1075 1 44 ............................. 16 0 951 .....................................A.........-N...........-........-.T..........N...N..A 1037 R V L R G F L L K Query_1 1076 GGGTCTTGAGGGGTTTTTTGCTGAAA 1101 0 1038 C..NN....N...G............ 1063 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3405815 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 3,187 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP91062.102 (IDP91062.26-266.P(266)..pMCSG68)
ADATSPVVTNSAFKNNDQQSAYALGASLGRYMDNSLKEQEKLGIKLDKDQLIAGVQDAFANKSKLTDEEIEKTLQNFEARVKASAQAKMEQDAKENADKGAKYRDTFAKEKGVKKTASGLLYKVENAGTGDAPKDSDTVVVNYKGTLADGTEFDNSYKRGEPLSFRLDGVIPGWTEGLKQIKKGGKITLVIPPELAYGKAGVPGIPANSTLIFDVELLDVKAAAKADAQEQQPAVDTKAKK
GCTGACGCAACCTCACCAGTAGTAACCAATAGCGCATTTAAAAATAATGATCAGCAATCTGCTTATGCATTAGGTGCGTCTTTGGGCCGCTATATGGATAATTCCCTGAAAGAACAAGAAAAATTGGGGATTAAGTTGGATAAAGACCAGTTGATCGCTGGTGTGCAAGATGCTTTTGCTAACAAAAGCAAACTGACTGACGAAGAGATCGAAAAAACCCTGCAAAATTTTGAAGCTCGGGTAAAAGCGTCTGCTCAAGCCAAAATGGAGCAAGATGCCAAAGAAAACGCGGATAAAGGCGCTAAGTACCGCGATACCTTTGCTAAAGAAAAAGGTGTGAAGAAAACCGCATCTGGCCTGCTGTACAAGGTAGAGAACGCCGGTACGGGTGATGCACCAAAAGACAGCGATACTGTTGTCGTTAACTACAAAGGCACTTTGGCTGACGGAACTGAGTTTGATAACTCATACAAACGTGGCGAGCCATTGTCTTTCCGTCTGGATGGCGTTATCCCAGGCTGGACTGAAGGCCTGAAGCAAATCAAAAAAGGTGGCAAAATCACCTTGGTTATCCCGCCAGAACTGGCTTACGGCAAAGCAGGCGTCCCTGGTATTCCTGCCAACTCTACTCTGATATTCGATGTAGAACTGCTGGATGTGAAAGCCGCAGCTAAAGCCGATGCTCAAGAGCAACAGCCTGCGGTTGATACAAAAGCTAAGAAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,834 total letters Query= IDP91062.102 Length=1128 Score E Sequences producing significant alignments: (Bits) Value 10E_E4_IDP91062.102_T7term.seq 1752 0.0 04E_E4_IDP91062.102_T7short.seq 1624 0.0 wt 1305 0.0 R S R E I N T T H Y R G I V S G * Q F P S R N N F V * Query_1 3 TCGAT-CCCGCGAAATTAATACGACTCA--CTATAGGGG-AATTGTGAGCGGATAAC-AATTCCCCTC-TAGAAA-TAATTTTGTTTAAC 85 1 1064 .....T.....................CAC.........G.................C..........-......-............C- 978 0 22 ......C......N.............A 49 L * E G D I H M H H H H H H S S G V D L W S H P Q F E K G Query_1 86 --TTTAAGAAGGAGATAT-ACATATGCACC-ATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGT 171 1 977 ---...............N...........M..............................N............................ 891 0 50 CT................-...........-........................................................... 137 T E N L Y F Q S N A A D A T S P V V T N S A F K N N D Q Q Query_1 172 A-CCGAGAACCTGTACTTCCAA-TCCAATGCCGCTGACGCAACCTCACCAGTAGTAACCAATAGCGCATTTAAAAATAATGATCAGCAAT 259 1 890 .A....................N.................................N................................. 801 0 138 .-....................-................................................................... 225 2 1 .......................................................... 58 S A Y A L G A S L G R Y M D N S L K E Q E K L G I K L D K Query_1 260 CTGCTTATGCATT-AGGTGCGTCTTTGGGCCGCTATATGGATAATTCCCTGAAAGAACAA-GAAAAATTGGGGATTAAGTTGGATAAA-G 346 1 800 .............T...........-..................................A...........................A. 712 0 226 .............-..............................................-...........................-. 312 2 59 .............-..............................................-...........................-. 145 D Q L I A G V Q D A F A N K S K L T D E E I E K T L Q N F E Query_1 347 ACCAGTTGATCGCTGGTGTGCAAGATGCTTTTGCTAACAAAAGCAAACTGACTGACGAAGAGATCGAAAAAACCCTGCAAAATTTTGAAG 436 1 711 .......................................................................................... 622 0 313 .......................................................................................... 402 2 146 .......................................................................................... 235 A R V K A S A Q A K M E Q D A K E N A D K G A K Y R D T F A Query_1 437 CTCGGGTAAAAGCGTCTGCTCAAGCCAAAATGGAGCAAGATGCCAAAGAAAACGCGGATAAAGGCGCTAAGTACCGCGATACCTTTGCTA 526 1 621 .......................................................................................... 532 0 403 .......................................................................................... 492 2 236 .......................................................................................... 325 K E K G V K K T A S G L L Y K V E N A G T G D A P K D S D T Query_1 527 AAGAAAAAGGTGTGAAGAAAACCGCATCTGGCCTGCTGTACAAGGTAGAGAACGCCGGTACGGGTGATGCACCAAAAGACAGCGATACTG 616 1 531 .......................................................................................... 442 0 493 .......................................................................................... 582 2 326 .......................................................................................... 415 V V V N Y K G T L A D G T E F D N S Y K R G E P L S F R L D Query_1 617 TTGTCGTTAACTACAAAGGCACTTTGGCTGACGGAACTGAGTTTGATAACTCATACAAACGTGGCGAGCCATTGTCTTTCCGTCTGGATG 706 1 441 .......................................................................................... 352 0 583 .......................................................................................... 672 2 416 .......................................................................................... 505 G V I P G W T E G L K Q I K K G G K I T L V I P P E L A Y Query_1 707 GCGTTATCCCAGGCTGGACTGAAGGCCTGAAGCAAATCAAAAAAGGTGGC-AAAATCACCTTGGTT-ATCCCGCCAG-AACTGGCTTACG 793 1 351 ..................................................-...............-..........-............ 265 0 673 ..................N...............................A...............A..........A............ 762 2 506 ..................................................-...............-..........-............ 592 G K A G V P G I P A N S T L I F D V E L L D V K A A A K A Query_1 794 GCAAAGCAGGCGTCCCTGGTATTCCTGCCAAC-TCTACTCTGATATTCGATGTAGAACTGC-TGGATGTGAAAGCCGCAGC-TAAAGCCG 880 1 264 ................................-............................-...................-........ 178 0 763 ........-.......................T....................T...N...T................N..T........ 851 2 593 ................................-............................-...................-........ 679 D A Q E Q Q P A V D T K A K K * H W K W I T D P N S S S V D Query_1 881 ATGCTCAAGAGCAACAGCCTG-CGGTTGATACAAAAGCTAAGAAGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGAC 969 1 177 .....................-.................................................................... 89 0 852 .................T...C..T..............N.....C....-....................................... 940 2 680 .....................-....................... 723 K L A A A L E H H H H H H * D P A A N K A R K E A E L A Query_1 970 AAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccac-TGAGATCCGGC-TGCTAACAAAGCCC--GAAAGGAAGCTGAG-TTGGCTG 1054 1 88 .....N.................................-...........-..............CGA........ 14 0 941 ..................N.......T........T...T...A.......T......-T..TT..--..............T....... 1027 A Query_1 1055 CTG 1057 0 1028 ... 1030 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3104157 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 2,834 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP91528.102 (IDP91528.24-261.P(261)..pMCSG68)
ADTASASLSDAQKKEIEKVIHDYLINNPEVLLEASQALQQKQQQNMQQQAQAAIQENAEQVFQGKLTTVGNPKGNVTLVEFFDYQCIHCKKMASTIENLVKKDSGLRVIYKEFPIFGKTSDLASRVALAAGMQGKYQAMHNALITIDKRLDEKTVMDAAKSIGLDMQKLKKDMDSQEVTDILDANRQLAEKLHLMGTPAFIIGSTPDGQYKKGSEISFIPGATSEQSLRELIKKAAGN
GCTGATACGGCTAGCGCTTCTTTATCAGATGCTCAAAAAAAGGAAATTGAAAAAGTCATTCATGATTATCTGATTAATAACCCGGAAGTTTTATTGGAGGCTTCGCAAGCATTGCAACAAAAACAGCAACAAAATATGCAACAGCAAGCTCAAGCCGCAATTCAGGAAAATGCTGAACAAGTTTTTCAAGGCAAATTAACTACTGTTGGTAATCCTAAAGGGAATGTAACACTGGTTGAGTTTTTTGATTATCAATGCATTCATTGCAAAAAAATGGCATCTACCATTGAAAATCTGGTTAAAAAAGACAGCGGATTACGAGTTATATACAAAGAATTTCCAATCTTTGGCAAGACTTCTGATCTGGCTTCCAGAGTTGCTCTGGCGGCTGGCATGCAAGGCAAATATCAGGCTATGCATAATGCTTTGATTACGATAGACAAACGTTTGGATGAAAAAACAGTGATGGATGCCGCAAAATCCATAGGTTTGGATATGCAAAAGCTGAAAAAAGATATGGATAGTCAGGAAGTGACCGATATCCTGGATGCGAATCGCCAATTGGCTGAAAAATTACATTTGATGGGTACACCAGCCTTTATAATTGGCTCAACACCAGATGGTCAATATAAAAAGGGCAGTGAAATATCATTTATACCAGGCGCTACAAGCGAGCAATCACTACGTGAGTTAATTAAAAAGGCGGCTGGCAAT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,814 total letters Query= IDP91528.102 Length=1119 Score E Sequences producing significant alignments: (Bits) Value 03E_E3_IDP91528.102_T7short.seq 1609 0.0 09E_E3_IDP91528.102_T7term.seq 1463 0.0 wt 1288 0.0 Q F P S R N N F V * L * E G D I H M H H H H H H S S G V D Query_1 55 CAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGAT-CT 143 0 14 ..T.....T.......-...................................................C...A..............-.. 101 1 919 ....A.. 913 L W S H P Q F E K G T E N L Y F Q S N A A D T A S A S L S D Query_1 144 GTGGTCTCATCCGCAGTTCGAAAA--GGGTACCGAGAACCTGTACTTCCAATCCAATGCCGCTGATACGGCTAGCGCTTCTTTATCAGAT 231 0 102 ........................--................................................................ 189 1 912 .N...N.......N..........AG................N....N........N............N.........N.......... 823 2 1 .............................. 30 A Q K K E I E K V I H D Y L I N N P E V L L E A S Q A L Q Query_1 232 GCTCaaaaaaa-GGAAATTGAAAAAGTCATTCATGATTATCTGATTAATAACCCGGAA-GTTTTATTGG-AGGCTTCGCAAGCATTGCAA 318 0 190 ..C........-..............................................-..........-................A... 276 1 822 ..C........A..............................................A..........G...N............A... 733 2 31 ...........-..............................................-..........-.................... 117 Q K Q Q Q N M Q Q Q A Q A A I Q E N A E Q V F Q G K L T T Query_1 319 CAAAAACAGCAACAAAA-TATGCAACAGCAAGCTCAAGCCGCAATTCAGGAAAATGCTGAACAAGTTTTTCAAGGCAAATTAACTACTGT 407 0 277 ...........G.....-.....................T.......................G.......................... 365 1 732 ...........G.....A.....................T.......................G.......................... 643 2 118 .................-........................................................................ 206 V G N P K G N V T L V E F F D Y Q C I H C K K M A S T I E N Query_1 408 TGGTAATCCTAAAGGGAATGTAACACTGGTTGAGTTTTTTGATTATCAATGCATTCATTGCaaaaaaaTGGCATCTACCATTGAAAATCT 497 0 366 A......................................................................................... 455 1 642 A......................................................................................... 553 2 207 .......................................................................................... 296 L V K K D S G L R V I Y K E F P I F G K T S D L A S R V A L Query_1 498 GGTTAAAAAAGACAGCGGATTACGAGTTATATACAAAGAATTTCCAATCTTTGGCAAGACTTCTGATCTGGCTTCCAGAGTTGCTCTGGC 587 0 456 .......................................................................................... 545 1 552 .......................................................................................... 463 1 147 ... 145 2 297 .......................................................................................... 386 2 702 ... 704 A A G M Q G K Y Q A M H N A L I T I D K R L D E K T V M D A Query_1 588 GGCTGGCATGCAAGGCAAATATCAGGCTATGCATAATGCTTTGATTACGATAGACAAACGTTTGGATGAAAAAACAGTGATGGATGCCGC 677 0 546 ...............A.......................................................................... 635 1 462 ...............A.......................................................................... 373 1 144 ........ 137 2 387 .......................................................................................... 476 2 705 ........ 712 A K S I G L D M Q K L K K D M D S Q E V T D I L D A N R Q L Query_1 678 AAAATCCATAGGTTTGGATATGCAAAAGCTGAAAAAAGATATGGATAGTCAGGAAGTGACCGATATCCTGGATGCGAATCGCCAATTGGC 767 0 636 C............C.............A.....T...................................................C.... 725 1 372 C............C.............A.........................................................C.... 283 2 477 .......................................................................................... 566 A E K L H L M G T P A F I I G S T P D G Q Y K K G S E I Query_1 768 TG-AAAAATTACATTTGATGGGTACACCAGCC--TTTATAATTGGCTCAACACCAGATGGTC-AATATAAAAA--GGGCAGTG-AAATAT 850 0 726 ..A..........................NN.CT..........................G.A..........AG..C.....A...A.. 815 1 282 ..-.............................--..........................G.-..........--........-...... 200 2 567 ..-.............................--............................-..........--........-...... 649 S F I P G A T S E Q S L R E L I K K A A G N * H W K W I T Query_1 851 CA-TTTATACCAGGCGC-TACAAGCGAGC-AATCACTACGTGAGTTAATT-AAAAAGGCGGCTGGCAATTAACATTGGAAGTGGATAACG 936 0 816 ..T..............T...N.......A....................A.........N............N.......N........ 905 0 543 ........... 553 1 199 ..-..............-...........-....................-....................................... 114 1 465 ........... 455 2 650 ..-..............-...........-....................-.................. 714 2 384 ........... 394 D P N S S S V D K L A A A L E H H H H H H * D P A A N K A Query_1 937 G-ATCCG-AATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCC 1024 0 906 .A.....A...............A.N.....N............A..T..........N.........A.........NA.......... 995 1 113 .-.....-......................N....................................................... 30 1 75 .......... 84 R K E A E L A A A T A E Q * L A * P L G A S K R V L R G Query_1 1025 GAAAGGAAGCTG-AGTTGGCTGCTGCCACCGCTGAGCAATAACTA-GCATAACCCC---TTGGGGCCTCTAAACGGGTCTTGAGGGGTTT 1109 0 996 .........T..A.T.....C......N.......A.....N...NN.....N...CTN.G........NN.......NA.....N.... 1085 F L L Query_1 1110 TTTG-CTGAA 1118 0 1086 ....N..... 1095 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3056976 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 2,814 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95771.103 (IDP95771.19-628.P(628)..pMCSG68)
AKKLAKKYKKDKDVNITLIDRHSYHTMMTELHEVAGGRVEPTAIQYDLRRLFNRTKVNLVTDNVTHVDHDKKVVTTEHGSYPFDYLVLGMGGEPNDFGTPGVGENGFTLWSWEDSVKLRNHIEETVTKASREQDVEKRKAMLTFVVCGSGFTGIEMVGELLEWKDRLAKDNKIDASEIKLVVVEAAPTILNMLERRDADKAERYMVKKGIEIMKNAAIVEVKPESIVLKSGEELPTSTLIWTAGVRANSDTKDYGMESARAGRLKVNQYMEAEGLKDVYVVGDLAYFEDEEGKPTPQIVEGAEQTALTAAKSIIVEMSGTGEKEPFQGKYHGVMVSIGAKYGVAHLGGMHLSGWFAILMKHMVNLYYFFGIRSGYYMWQYIMHEFFHIKDHRNIFRGWTSRYGNVLWVLPLRVYLGWFWIDEALSKIYGETTWDKVSITNLKPLFNGIGSDSWLTATTSKMPFEWLQTAATSGASQAAGDAAGAAATNVTTPILSHMPGWFQWIMELLMPNLDVALVMQKVVPFVELAIGLAMVVGLFTWLVSIGSAGFLVMFTLSAMLGWDKFWALPASIALLNGAGRTFGLDYWAVPWFQKHLGHWWYGKPRSVYRDK
GCTAAGAAATTAGCTAAGAAATACAAGAAAGACAAAGACGTTAATATTACATTAATCGATCGTCATTCGTACCATACAATGATGACTGAACTACATGAGGTTGCTGGTGGTCGTGTTGAACCAACTGCAATTCAATATGATTTACGTCGTTTGTTTAATAGAACAAAAGTTAATCTTGTAACTGATAACGTGACACATGTAGATCATGATAAAAAAGTTGTAACAACAGAGCACGGTAGTTATCCGTTTGATTACCTAGTACTTGGTATGGGCGGAGAACCTAATGATTTCGGAACTCCTGGTGTTGGCGAAAATGGCTTTACACTTTGGTCTTGGGAAGATTCTGTTAAGTTACGCAATCATATTGAAGAAACTGTAACGAAAGCATCTCGCGAACAAGACGTTGAAAAACGTAAAGCGATGTTAACATTCGTTGTTTGTGGATCTGGATTTACTGGTATCGAAATGGTTGGGGAACTTTTAGAATGGAAAGATCGTCTTGCTAAAGATAACAAAATTGACGCATCTGAAATTAAACTAGTTGTAGTAGAAGCTGCTCCAACAATCCTAAACATGCTTGAAAGAAGAGACGCTGACAAAGCAGAACGTTACATGGTTAAAAAAGGTATTGAAATCATGAAAAATGCTGCTATCGTTGAAGTTAAACCTGAAAGCATCGTACTTAAATCTGGCGAAGAACTTCCAACAAGCACATTAATTTGGACTGCTGGTGTTCGCGCTAACTCCGATACAAAAGATTACGGTATGGAATCTGCTCGTGCAGGCCGTTTGAAAGTAAACCAATATATGGAAGCAGAAGGTCTTAAAGACGTTTATGTTGTTGGTGACCTTGCTTACTTTGAAGATGAAGAAGGCAAACCAACTCCGCAAATCGTTGAAGGTGCTGAACAAACTGCACTAACTGCAGCGAAAAGTATCATCGTTGAAATGAGCGGTACTGGCGAGAAAGAACCATTCCAAGGTAAATACCATGGTGTAATGGTTTCTATTGGAGCTAAATATGGTGTTGCTCATCTTGGTGGCATGCATCTTTCTGGTTGGTTCGCTATTTTAATGAAACATATGGTTAACTTGTACTACTTCTTCGGTATCCGTAGTGGTTACTACATGTGGCAATATATTATGCATGAATTCTTCCACATTAAAGATCACCGTAATATCTTCCGTGGTTGGACTTCTCGTTACGGTAACGTACTTTGGGTTCTTCCTTTACGTGTATATCTAGGTTGGTTCTGGATTGACGAAGCTCTTTCTAAAATCTACGGTGAAACTACATGGGATAAAGTAAGTATCACAAATTTAAAACCTTTATTTAACGGTATCGGTTCTGACTCTTGGTTAACTGCAACTACCTCTAAAATGCCATTCGAATGGTTACAAACTGCTGCTACATCTGGTGCTAGTCAAGCTGCCGGTGATGCTGCTGGGGCTGCTGCAACAAACGTAACTACTCCAATTCTTAGTCATATGCCTGGTTGGTTCCAGTGGATTATGGAACTTCTAATGCCAAATCTTGATGTTGCTCTAGTAATGCAAAAAGTTGTACCTTTTGTTGAACTTGCAATCGGTCTTGCTATGGTAGTAGGTCTATTCACTTGGCTTGTAAGTATCGGAAGTGCTGGATTCCTAGTAATGTTTACACTAAGCGCTATGCTTGGTTGGGACAAATTCTGGGCGTTACCAGCTTCTATCGCACTTCTAAATGGCGCTGGGCGTACATTTGGTCTTGATTATTGGGCTGTTCCTTGGTTCCAAAAACATCTTGGTCATTGGTGGTACGGTAAGCCGAGATCCGTTTACAGAGACAAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,355 total letters Query= IDP95771.103 Length=2235 Score E Sequences producing significant alignments: (Bits) Value wt 3301 0.0 09F_F3_IDP95771.103_T7term.seq 141 1e-36 A K K L A K K Y K K D K D V N I T L I D R H S Y H T M M T E Query_1 202 GCTAAGAAATTAGCTAAGAAATACAAGAAAGACAAAGACGTTAATATTACATTAATCGATCGTCATTCGTACCATACAATGATGACTGAA 291 2 1 .......................................................................................... 90 L H E V A G G R V E P T A I Q Y D L R R L F N R T K V N L V Query_1 292 CTACATGAGGTTGCTGGTGGTCGTGTTGAACCAACTGCAATTCAATATGATTTACGTCGTTTGTTTAATAGAACAAAAGTTAATCTTGTA 381 2 91 .......................................................................................... 180 2 369 ............ 358 T D N V T H V D H D K K V V T T E H G S Y P F D Y L V L G M Query_1 382 ACTGATAACGTGACACATGTAGATCATGATAAAAAAGTTGTAACAACAGAGCACGGTAGTTATCCGTTTGATTACCTAGTACTTGGTATG 471 2 181 .......................................................................................... 270 2 1556 ........... 1566 G G E P N D F G T P G V G E N G F T L W S W E D S V K L R N Query_1 472 GGCGGAGAACCTAATGATTTCGGAACTCCTGGTGTTGGCGAAAATGGCTTTACACTTTGGTCTTGGGAAGATTCTGTTAAGTTACGCAAT 561 2 271 .......................................................................................... 360 2 142 ... 140 H I E E T V T K A S R E Q D V E K R K A M L T F V V C G S G Query_1 562 CATATTGAAGAAACTGTAACGAAAGCATCTCGCGAACAAGACGTTGAAAAACGTAAAGCGATGTTAACATTCGTTGTTTGTGGATCTGGA 651 2 361 .......................................................................................... 450 2 139 ......... 131 F T G I E M V G E L L E W K D R L A K D N K I D A S E I K L Query_1 652 TTTACTGGTATCGAAATGGTTGGGGAACTTTTAGAATGGAAAGATCGTCTTGCTAAAGATAACAAAATTGACGCATCTGAAATTAAACTA 741 2 451 .......................................................................................... 540 V V V E A A P T I L N M L E R R D A D K A E R Y M V K K G I Query_1 742 GTTGTAGTAGAAGCTGCTCCAACAATCCTAAACATGCTTGAAAGAAGAGACGCTGACAAAGCAGAACGTTACATGGTTAAAAAAGGTATT 831 2 541 .......................................................................................... 630 E I M K N A A I V E V K P E S I V L K S G E E L P T S T L I Query_1 832 GAAATCATGAAAAATGCTGCTATCGTTGAAGTTAAACCTGAAAGCATCGTACTTAAATCTGGCGAAGAACTTCCAACAAGCACATTAATT 921 2 631 .......................................................................................... 720 W T A G V R A N S D T K D Y G M E S A R A G R L K V N Q Y M Query_1 922 TGGACTGCTGGTGTTCGCGCTAACTCCGATACAAAAGATTACGGTATGGAATCTGCTCGTGCAGGCCGTTTGAAAGTAAACCAATATATG 1011 2 721 .......................................................................................... 810 E A E G L K D V Y V V G D L A Y F E D E E G K P T P Q I V E Query_1 1012 GAAGCAGAAGGTCTTAAAGACGTTTATGTTGTTGGTGACCTTGCTTACTTTGAAGATGAAGAAGGCAAACCAACTCCGCAAATCGTTGAA 1101 2 811 .......................................................................................... 900 G A E Q T A L T A A K S I I V E M S G T G E K E P F Q G K Y Query_1 1102 GGTGCTGAACAAACTGCACTAACTGCAGCGAAAAGTATCATCGTTGAAATGAGCGGTACTGGCGAGAAAGAACCATTCCAAGGTAAATAC 1191 2 901 .......................................................................................... 990 1 236 .......... 227 H G V M V S I G A K Y G V A H L G G M H L S G W F A I L M K Query_1 1192 CATGGTGTAATGGTTTCTATTGGAGCTAAATATGGTGTTGCTCATCTTGGTGGCATGCATCTTTCTGGTTGGTTCGCTATTTTAATGAAA 1281 2 991 .......................................................................................... 1080 2 1493 ........... 1503 H M V N L Y Y F F G I R S G Y Y M W Q Y I M H E F F H I K D Query_1 1282 CATATGGTTAACTTGTACTACTTCTTCGGTATCCGTAGTGGTTACTACATGTGGCAATATATTATGCATGAATTCTTCCACATTAAAGAT 1371 2 1081 .......................................................................................... 1170 H R N I F R G W T S R Y G N V L W V L P L R V Y L G W F W I Query_1 1372 CACCGTAATATCTTCCGTGGTTGGACTTCTCGTTACGGTAACGTACTTTGGGTTCTTCCTTTACGTGTATATCTAGGTTGGTTCTGGATT 1461 2 1171 .......................................................................................... 1260 D E A L S K I Y G E T T W D K V S I T N L K P L F N G I G S Query_1 1462 GACGAAGCTCTTTCTAAAATCTACGGTGAAACTACATGGGATAAAGTAAGTATCACAAATTTAAAACCTTTATTTAACGGTATCGGTTCT 1551 2 1261 .......................................................................................... 1350 D S W L T A T T S K M P F E W L Q T A A T S G A S Q A A G D Query_1 1552 GACTCTTGGTTAACTGCAACTACCTCTAAAATGCCATTCGAATGGTTACAAACTGCTGCTACATCTGGTGCTAGTCAAGCTGCCGGTGAT 1641 2 1351 .......................................................................................... 1440 2 1395 ............ 1384 A A G A A A T N V T T P I L S H M P G W F Q W I M E L L M P Query_1 1642 GCTGCTGGGGCTGCTGCAACAAACGTAACTACTCCAATTCTTAGTCATATGCCTGGTTGGTTCCAGTGGATTATGGAACTTCTAATGCCA 1731 2 1441 .......................................................................................... 1530 2 1055 ........... 1065 N L D V A L V M Q K V V P F V E L A I G L A M V V G L F T W Query_1 1732 AATCTTGATGTTGCTCTAGTAATGCAAAAAGTTGTACCTTTTGTTGAACTTGCAATCGGTCTTGCTATGGTAGTAGGTCTATTCACTTGG 1821 2 1531 .......................................................................................... 1620 2 212 ........... 222 1 209 ........... 199 L V S I G S A G F L V M F T L S A M L G W D K F W A L P A S Query_1 1822 CTTGTAAGTATCGGAAGTGCTGGATTCCTAGTAATGTTTACACTAAGCGCTATGCTTGGTTGGGACAAATTCTGGGCGTTACCAGCTTCT 1911 2 1621 .......................................................................................... 1710 I A L L N G A G R T F G L D Y W A V P W F Q K H L G H W W Y Query_1 1912 ATCGCACTTCTAAATGGCGCTGGGCGTACATTTGGTCTTGATTATTGGGCTGTTCCTTGGTTCCAAAAACATCTTGGTCATTGGTGGTAC 2001 2 1711 .......................................................................................... 1800 G K P R S V Y R D K * H W K W I T D P N S S S V D K L A A A Query_1 2002 GGTAAGCCGAGATCCGTTTACAGAGACAAGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCA 2091 2 1801 .............................. 1830 1 128 ..........................................TT.TT...G......-.. 70 L E H H H H H H * D P A A N K A R K E Query_1 2092 CTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAA 2148 1 69 ......---...........A..N.....................N..NN...A... 16 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5119233 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 2,355 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95803.102 (IDP95803.1-353.F..pMCSG68)
MNQSASEQLQTDIPASISAMVLLNSACQGVVETYIDQGNAEHWYAQVEQNLNAVQKLVRQWRLSGNLYFSNDIMDSVLSIANTFKDSNVQILTLFKALETRFDTAQLQQLTSLILTLQNPIQSLTSNIKRYDEGLNAWARQVEDAHNTLQQTIAQIQQEEVSIQAEIIATNAQIDLMKQQIAAFKTAIANAQSQRKKGIFETIFGVVLAPFTLGGSLILAGFGVSSIVEAQSEISSLQSDIQSSLNTINHDQQTLSQDQQQIASLNALLLSVDQVNNDCAAISRSLDTLQTTVLSLYNETNNVVSNLTKAQDSQAVILEQVWYQSAYNEWQDILEVASTLNNAQPQITKAQIK
ATGAATCAATCAGCCAGCGAGCAACTTCAAACCGACATTCCTGCATCTATTAGCGCTATGGTGTTACTAAATTCTGCATGCCAAGGCGTTGTTGAAACTTATATTGATCAAGGCAATGCAGAACATTGGTATGCTCAGGTAGAACAAAATTTAAATGCGGTGCAAAAACTGGTACGACAATGGCGTTTGAGTGGTAATTTATATTTTTCAAATGACATTATGGACAGTGTTCTTTCCATTGCGAATACATTCAAAGATTCTAATGTTCAAATTTTAACGTTATTCAAAGCACTAGAAACCCGTTTTGATACCGCCCAACTACAGCAGCTCACTTCATTAATCTTAACATTACAAAATCCGATTCAAAGTCTTACATCAAATATAAAACGTTATGATGAGGGATTGAATGCTTGGGCTCGCCAAGTGGAAGATGCTCATAACACATTGCAACAAACTATTGCACAGATCCAGCAAGAAGAAGTTTCAATCCAAGCAGAAATTATCGCCACCAACGCCCAAATTGATCTAATGAAACAGCAGATAGCAGCATTTAAAACCGCCATTGCGAACGCTCAATCGCAGCGTAAGAAAGGGATATTTGAAACGATTTTTGGCGTGGTGCTTGCTCCGTTTACACTGGGAGGTTCTTTGATATTGGCAGGGTTTGGTGTTAGCTCTATTGTAGAAGCACAAAGTGAAATATCTAGCCTGCAAAGCGATATCCAATCATCTTTAAATACCATCAACCACGATCAACAAACGCTCTCTCAAGATCAACAACAAATCGCGTCTCTCAATGCCCTATTGCTAAGTGTCGATCAAGTTAACAACGATTGTGCGGCGATCAGCCGATCCTTAGATACACTACAAACCACGGTGCTCAGTTTATACAATGAAACCAACAATGTCGTTTCTAACCTTACCAAAGCACAAGATTCACAAGCGGTCATTCTGGAGCAAGTTTGGTACCAATCAGCTTACAATGAGTGGCAAGATATTCTGGAGGTAGCATCAACGCTCAATAATGCACAACCTCAAATAACAAAAGCTCAAATCAAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,902 total letters Query= IDP95803.102 Length=1464 Score E Sequences producing significant alignments: (Bits) Value wt 1911 0.0 11C_C5_IDP95803.102_T7term.seq 1510 0.0 05C_C5_IDP95803.102_T7short.seq 1494 0.0 V S G * Q F P S R N N F V * L * E G D I H M H H H H H H Query_1 42 TGTGAGCGGATAACAATT--CCCCTCTAGAAATAATTTT-GTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTC 128 1 734 ....N....-... 745 0 3 ......G...--.N....TC.......TN..........T..................N............................... 90 S S G V D L W S H P Q F E K G T E N L Y F Q S N A M N Q S A Query_1 129 TTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGAATCAATCAGCCAG 218 2 1 ................. 17 0 91 .......................................................................................... 180 S E Q L Q T D I P A S I S A M V L L N S A C Q G V V E T Y I Query_1 219 CGAGCAACTTCAAACCGACATTCCTGCATCTATTAGCGCTATGGTGTTACTAAATTCTGCATGCCAAGGCGTTGTTGAAACTTATATTGA 308 2 18 .......................................................................................... 107 0 181 .......................................................................................... 270 D Q G N A E H W Y A Q V E Q N L N A V Q K L V R Q W R L S G Query_1 309 TCAAGGCAATGCAGAACATTGGTATGCTCAGGTAGAACAAAATTTAAATGCGGTGCAAAAACTGGTACGACAATGGCGTTTGAGTGGTAA 398 2 108 .......................................................................................... 197 0 271 .............N.....................................................................N...... 360 N L Y F S N D I M D S V L S I A N T F K D S N V Q I L T L F Query_1 399 TTTATATTTTTCAAATGACATTATGGACAGTGTTCTTTCCATTGCGAATACATTCAAAGATTCTAATGTTCAAATTTTAACGTTATTCAA 488 2 198 .......................................................................................... 287 1 926 ........-...... 913 0 361 .........................................................................................N 450 K A L E T R F D T A Q L Q Q L T S L I L T L Q N P I Q S L Query_1 489 A-GCACTAGAAA-CCCGTTTTGATACCGCCCAACTACAGCAGCTCACTT-CATTAATCTTAACATTACAAAA-TCCGATTCAAAGTCTTA 574 2 288 .-..........-....................................-......................-................. 373 1 912 .A..........A.......N............................T.......N..............A................. 823 0 451 .-..........-....................................-......................-................. 536 T S N I K R Y D E G L N A W A R Q V E D A H N T L Q Q T I Query_1 575 CATCAAA-TATAAAACGTTATGATGAGGGATTGAATGCTTGGGCTCGCCAAGTGGAAGATG-CTCATAACACATTGCAACAAA-CTATTG 661 2 374 .......-.....................................................-.....................-...... 460 1 822 .......A......N.....N..........N.......G.....................G.....................AN..... 733 0 537 .......-.........................................N...........-.....................-...... 623 A Q I Q Q E E V S I Q A E I I A T N A Q I D L M K Q Q I Query_1 662 CACAGATCCAGCAAGAA-GAAGTTT-CAATCCAAGCAGAAATTATCGCCA-CCAACGCCCAAA-TTGATCTAATGAAA-CAGCAGATAGC 746 2 461 .................-.......-........................-............-..............-........... 545 1 732 .................A.......T........................-............A.....-........A........... 645 0 624 .................-.......-........................C...........N-..............-........... 709 A A F K T A I A N A Q S Q R K K G I F E T I F G V V L A P F Query_1 747 AGCATTTAAAACCGCCATTGCGAACGCTCAATCGCAGCGTAAGAAAGGGATATTTGAAACGATTTTTGGCGTGGTGCTTGCTCCGTTTAC 836 2 546 .......................................................................................... 635 1 644 .......................................................................................... 555 0 710 .....................N..............N.........................-..........N................ 798 T L G G S L I L A G F G V S S I V E A Q S E I S S L Q S D I Query_1 837 ACTGGGAGGTTCTTTGATATTGGCAGGGTTTGGTGTTAGCTCTATTGTAGAAGCACAAAGTGAAATATCTAG-CCTGCAAAGCGATATCC 925 2 636 ........................................................................-................. 724 1 554 ........................................................................-................. 466 0 799 .........-.............-.......-...-.....................N.N............C................. 884 Q S S L N T I N H D Q Q T L S Q D Q Q Q I A S L N A L L L Query_1 926 AATCAT-CTTTAAATACCAT-CAACCACGATCAACAAACGCTCTCTCAAGATCAACAACAAATCGCGTCTCTCAATGCCCTATTGCTAAG 1013 2 725 ......-.............-..................................................................... 812 1 465 ......-.............-..................................................................... 378 0 885 ......C...........N.C......... 914 S V D Q V N N D C A A I S R S L D T L Q T T V L S L Y N E T Query_1 1014 TGTCGATCAAGTTAACAACGATTGTGCGGCGATCAGCCGATCCTTAGATACACTACAAACCACGGTGCTCAGTTTATACAATGAAACCAA 1103 2 813 .......................................................................................... 902 1 377 .......................................................................................... 288 N N V V S N L T K A Q D S Q A V I L E Q V W Y Q S A Y N E W Query_1 1104 CAATGTCGTTTCTAACCTTACCAAAGCACAAGATTCACAAGCGGTCATTCTGGAGCAAGTTTGGTACCAATCAGCTTACAATGAGTGGCA 1193 2 903 .......................................................................................... 992 1 287 .......................................................................................... 198 Q D I L E V A S T L N N A Q P Q I T K A Q I K * H W K W I T Query_1 1194 AGATATTCTGGAGGTAGCATCAACGCTCAATAATGCACAACCTCAAATAACAAAAGCTCAAATCAAGTAACATTGGAAGTGGATAACGGA 1283 2 993 ................................................................... 1059 1 197 .......................................................................................... 108 0 732 .......... 741 D P N S S S V D K L A A A L E H H H H H H * D P A A N K A R Query_1 1284 TCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAA 1373 1 107 ........................A................................................................. 18 K E Query_1 1374 GGAA 1377 1 17 A... 14 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4132592 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 2,902 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95810.102 (IDP95810.1-377.F..pMCSG68)
MMEFTIKRDYFITQLNDTLKAISPRTTLPILTGIKIDAKEHEVILTGSDSEISIEITIPKTVDGEDIVNISETGSVVLPGRFFVDIIKKLPGKDVKLSTNEQFQTLITSGHSEFNLSGLDPDQYPLLPQVSRDDAIQLSVKVLKNVIAQTNFAVSTSETRPVLTGVNWLIQENELICTATDSHRLAVRKLQLEDVSENKNVIIPGKALAELNKIMSDNEEDIDIFFASNQVLFKVGNVNFISRLLEGHYPDTTRLFPENYEIKLSIDNGEFYHAIDRASLLAREGGNNVIKLSTGDDVVELSSTSPEIGTVKEEVDANDVEGGSLKISFNSKYMMDALKAIDNDEVEVEFFGTMKPFILKPKGDDSVTQLILPIRTY
ATGATGGAATTCACTATTAAAAGAGATTATTTTATTACACAATTAAATGACACATTAAAAGCTATTTCACCAAGAACAACATTACCTATATTAACTGGTATCAAAATCGATGCGAAAGAACATGAAGTTATATTAACTGGTTCAGACTCTGAAATTTCAATAGAAATCACTATTCCTAAAACTGTAGATGGCGAAGATATTGTCAATATTTCAGAAACAGGCTCAGTAGTACTTCCTGGACGATTCTTTGTTGATATTATAAAAAAATTACCTGGTAAAGATGTTAAATTATCTACAAATGAACAATTCCAGACATTAATTACATCAGGTCATTCTGAATTTAATTTAAGTGGCTTAGATCCAGATCAATATCCTTTATTACCTCAAGTTTCTAGAGATGACGCAATTCAATTGTCGGTAAAAGTGCTTAAAAACGTGATTGCACAAACAAATTTTGCAGTGTCCACCTCAGAAACACGCCCAGTACTAACTGGTGTGAACTGGCTTATACAAGAAAATGAATTAATATGCACAGCGACTGACTCACACCGCTTGGCTGTAAGAAAGTTGCAGTTAGAAGATGTTTCTGAAAACAAAAATGTCATCATTCCAGGTAAGGCTTTAGCTGAATTAAATAAAATTATGTCTGACAATGAAGAAGACATTGATATCTTCTTTGCTTCAAACCAAGTTTTATTTAAAGTTGGAAATGTGAACTTTATTTCTCGATTATTAGAAGGACATTATCCTGATACAACACGTTTATTCCCTGAAAACTATGAAATTAAATTAAGTATAGACAATGGGGAGTTTTATCATGCGATTGATCGTGCCTCTTTATTAGCGCGTGAAGGTGGTAATAACGTTATTAAATTAAGTACAGGTGATGACGTTGTTGAATTGTCTTCTACATCACCAGAAATTGGTACTGTAAAAGAAGAAGTTGATGCAAACGATGTTGAAGGTGGTAGCCTGAAAATTTCATTCAACTCTAAATATATGATGGATGCTTTAAAAGCAATCGATAATGATGAGGTTGAAGTTGAATTCTTCGGTACAATGAAACCATTTATTCTAAAACCAAAAGGTGACGACTCGGTAACGCAATTAATTTTACCAATCAGAACTTAC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,283 total letters Query= IDP95810.102 Length=1536 Score E Sequences producing significant alignments: (Bits) Value wt 2040 0.0 10D_D4_IDP95810.102_T7term.seq 1831 0.0 M M E F T I K R D Y F I T Q L N D T L K A I S P R T T L P I Query_1 202 ATGATGGAATTCACTATTAAAAGAGATTATTTTATTACACAATTAAATGACACATTAAAAGCTATTTCACCAAGAACAACATTACCTATA 291 2 1 .......................................................................................... 90 L T G I K I D A K E H E V I L T G S D S E I S I E I T I P Query_1 292 TTAACTGGTATCAAAATCGATGCGAAAG-AACATGAAGTTATATTAACTGGTTCAGACTCTGAAATTTCAATAGAAATCACTATTCCTAA 380 2 91 ............................-............................................................. 179 1 1147 .......A....N.....N.-......CNTNNN...-...N..........-..............N.-....N 1078 K T V D G E D I V N I S E T G S V V L P G R F F V D I I K K Query_1 381 AACTGTAGATGGCGAAGATATTGTCAATATTTCAGAAACAGGCTCAGTAGTACTTCCTGGACGATTCTTTGTTGATATTATaaaaaaaTT 470 2 180 .......................................................................................... 269 2 592 ........... 582 1 1077 ...........-...G....N....C...........-...-...........................G........ATA.......A. 991 1 674 ........... 684 L P G K D V K L S T N E Q F Q T L I T S G H S E F N L S G L Query_1 471 ACCTGGTAAAGATGTTAAATTATCTACAAATGAACAATTCCAGACATTAATTACATCAGGTCATTCTGAATTTAATTTAAGTGGCTTAGA 560 2 270 .......................................................................................... 359 1 990 .........-..N.......-........-.................-.......................................... 905 D P D Q Y P L L P Q V S R D D A I Q L S V K V L K N V I A Q Query_1 561 TCCAGATCAATATCCTTTATTACCTCAAGTTTCTAGAGATGACGCAATTCAATTGTCGGTAAAAGTGCTTAAAAACGTGATTGCACAAAC 650 2 360 .......................................................................................... 449 1 904 .................-......................................N........................-........ 817 T N F A V S T S E T R P V L T G V N W L I Q E N E L I C T A Query_1 651 AAATTTTGCAGTGTCCACCTCAGAAACACGCCCAGTACTAACTGGTGTGAACTGGCTTATACAAGAAAATGAATTAATATGCACAGCGAC 740 2 450 .......................................................................................... 539 1 816 G......................................................................................... 727 T D S H R L A V R K L Q L E D V S E N K N V I I P G K A L A Query_1 741 TGACTCACACCGCTTGGCTGTAAGAAAGTTGCAGTTAGAAGATGTTTCTGAAAACAAAAATGTCATCATTCCAGGTAAGGCTTTAGCTGA 830 2 540 .......................................................................................... 629 2 219 ........... 209 1 726 .......................................................................................... 637 E L N K I M S D N E E D I D I F F A S N Q V L F K V G N V N Query_1 831 ATTAAATAAAATTATGTCTGACAATGAAGAAGACATTGATATCTTCTTTGCTTCAAACCAAGTTTTATTTAAAGTTGGAAATGTGAACTT 920 2 630 .......................................................................................... 719 1 636 .......................................................................................... 547 1 943 ........... 953 F I S R L L E G H Y P D T T R L F P E N Y E I K L S I D N G Query_1 921 TATTTCTCGATTATTAGAAGGACATTATCCTGATACAACACGTTTATTCCCTGAAAACTATGAAATTAAATTAAGTATAGACAATGGGGA 1010 2 720 .......................................................................................... 809 2 868 .............C.. 883 1 546 .......................................................................................... 457 1 398 .............C.. 383 E F Y H A I D R A S L L A R E G G N N V I K L S T G D D V V Query_1 1011 GTTTTATCATGCGATTGATCGTGCCTCTTTATTAGCGCGTGAAGGTGGTAATAACGTTATTAAATTAAGTACAGGTGATGACGTTGTTGA 1100 2 810 .......................................................................................... 899 2 784 .............T.. 799 2 871 .............. 858 2 960 ........... 970 1 456 ....................................A..................................................... 367 1 482 .............T.. 467 1 395 .............. 408 1 306 ........... 296 E L S S T S P E I G T V K E E V D A N D V E G G S L K I S F Query_1 1101 ATTGTCTTCTACATCACCAGAAATTGGTACTGTAAAAGAAGAAGTTGATGCAAACGATGTTGAAGGTGGTAGCCTGAAAATTTCATTCAA 1190 2 900 .......................................................................................... 989 2 849 ........... 859 1 366 ...A...................................................................................... 277 1 417 ........... 407 N S K Y M M D A L K A I D N D E V E V E F F G T M K P F I L Query_1 1191 CTCTAAATATATGATGGATGCTTTAAAAGCAATCGATAATGATGAGGTTGAAGTTGAATTCTTCGGTACAATGAAACCATTTATTCTAAA 1280 2 990 .......................................................................................... 1079 1 276 .......................................................................................... 187 K P K G D D S V T Q L I L P I R T Y * H W K W I T D P N S S Query_1 1281 ACCAAAAGGTGACGACTCGGTAACGCAATTAATTTTACCAATCAGAACTTACTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTC 1370 2 1080 .................................................... 1131 1 186 .......................................................................................... 97 S V D K L A A A L E H H H H H H * D P A A N K A Query_1 1371 CGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCC 1441 1 96 ..........................................................A............ 26 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3397200 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 2,283 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95851.103 (IDP95851.49-269.P(399)..pMCSG68)
NGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVVLQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGDQIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGNITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGL
AACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,735 total letters Query= IDP95851.103 Length=1068 Score E Sequences producing significant alignments: (Bits) Value 03D_D3_IDP95851.103_T7short.seq 1599 0.0 09D_D3_IDP95851.103_T7term.seq 1281 0.0 wt 1196 0.0 Q F P S R N N F V * L * E G D I H M H H H H H H S S G V Query_1 55 CAATTCCCCT-CT-AGAAATAATTTTG--TTTAACTTT-AAGAA-GGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTA 138 0 16 ..T.......T..N....NN......TGT.........T.....N............................................. 105 D L W S H P Q F E K G T E N L Y F Q S N A N G F E R E L Q N Query_1 139 GATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCAACGGCTTTGAGCGCGAGCTGCAAAAC 228 0 106 .......................................................................................... 195 2 1 ........................... 27 N I L G L M P Q A I L S S E H G S L N P Q Q L P E T A V K L Query_1 229 AACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTG 318 0 196 .......................................................................................... 285 1 759 ..N..................N.A.T.T.N..T.................................................... 675 2 28 .......................................................................................... 117 D G V N R V A P I T T G D V V L Q S A R S V A V G V M L G I Query_1 319 GACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATC 408 0 286 .......................................................................................... 375 0 487 ............. 499 1 674 .......................................................................................... 585 1 473 ............. 461 2 118 .......................................................................................... 207 2 319 ............. 331 D P A Q K D P L T P Y L V N V K Q T D L E P G K Y N V I L G Query_1 409 GACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGC 498 0 376 .......................................................................................... 465 1 584 ...........................................................N.............................. 495 2 208 .......................................................................................... 297 E Q L A S Q L G V N R G D Q I R V M V P S A S Q F T P M G R Query_1 499 GAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGT 588 0 466 .......................................................................................... 555 0 289 ............. 301 0 527 ............ 516 1 494 .......................................................................................... 405 1 671 ............. 659 1 433 ............ 444 2 298 .......................................................................................... 387 2 121 ............. 133 2 359 ............ 348 I P S Q R L F N V I G T F A A N S E V D G Y E M L V N I E D Query_1 589 ATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGAT 678 0 556 .......................................................................................... 645 1 404 ...................................................................................N...... 315 2 388 .......................................................................................... 477 A S R L M R Y P A G N I T G W R L W L D E P L K V D S L S Q Query_1 679 GCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAG 768 0 646 .......................................................................................... 735 1 314 .......................................................................................... 225 2 478 .......................................................................................... 567 Q K L P E G S K W Q D W R D R K G E L F Q A V R M E K N M Query_1 769 CAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTG-TTCCAGGCCGTACGCATGGaaaaaaaTATGAT 857 0 736 .....................N...............................A...T................................ 825 1 224 .........................................................-................................ 136 2 568 .........................................................-................................ 656 M G L * H W K W I T D P N S S S V D K L A A A L E H H H Query_1 858 GGG--TTTATAACATTGGAAGTGGATAACGGA-TCCGAATTCGAGCTCCGTCG-ACAAGC-TTGC-GGCCGCACT-CGAGcaccaccacc 940 0 826 ...GT........................N..T...................NA......T....G.........C.............- 914 1 135 ...--...........................-....................-......-....-.........-....---....... 56 2 657 ...--.... 663 H H H * D P A A N K A R K E A E L A A A T A E Q * L A * P Query_1 941 accacc-acTG-AGATCCGGCTGCTAACAAAG-CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTG-AGCAATAACTAGCATAACCC 1026 0 915 -.....TNN..A....................C...........N....A.............T.....A.N..N....N.CAT..T... 1003 1 55 ......-....-....................-.. 24 L G A S K R V L R G F Query_1 1027 CTT-GGGGCCTCTAAACGGGTCTTG-AGGGGTTTTTT 1061 0 1004 ...G............T........A......G.... 1040 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2832570 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 2,735 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95852.102 (IDP95852.1-422.F..pMCSG68)
MENKSIPQATSPLAAWLSYLENLHSKTIDMGLERVSQVAARLDVLKPAPFVFTVAGTNGKGTTCRTLESVLMAAGYKVGVYSSPHLVRYTERVRVQNTELAESAHTASFAAIEAARGETSLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNMVDADVAVVTSIALDHTDWLGPDRESIGREKAGIFRANKPAVVGEPDMPHTIAEVAKEKGARLLRRGVDWQYEVQDNGWRFSDAQGVLESLPLPQVPQPNAATALAALRASGLAVSEQAIRDGIQNALLPGRFQIVSESPRLILDVAHNPHAAAYLAGRLKSLPKTGRVLAVIGMLHDKDIGGTLACMESVVDSWYCAPLEGPRGATAEQLMEHLGKGEIYSSVVSAWRAAMAEAKPEDTVLVCGSFHTVAHVMEVMDAGRTGGE
ATGGAAAATAAAAGCATTCCTCAAGCCACGTCGCCCCTGGCCGCGTGGCTTTCTTATCTGGAAAACCTGCACAGTAAAACTATCGACATGGGCCTTGAGCGCGTAAGCCAGGTTGCCGCGCGTCTTGACGTGCTGAAACCCGCGCCTTTCGTGTTTACCGTCGCCGGCACTAACGGTAAAGGCACCACCTGCCGCACGCTGGAATCTGTCCTGATGGCGGCGGGGTACAAAGTTGGCGTTTACAGCTCGCCGCATCTGGTGCGCTATACCGAGCGCGTGCGGGTGCAAAACACCGAGCTGGCGGAATCGGCCCATACGGCGTCGTTTGCCGCTATCGAAGCGGCGCGCGGCGAAACCTCATTAACCTATTTCGAATATGGCACCCTGTCGGCGCTGTGGCTGTTTAAGCAGGCGCAGCTGGACGTGGTGATCCTGGAAGTGGGGCTGGGCGGGCGTCTGGATGCCACCAATATGGTGGATGCGGATGTCGCCGTCGTCACCAGCATCGCGCTGGATCACACCGACTGGCTGGGCCCGGACCGCGAGAGCATTGGCCGTGAGAAGGCCGGTATTTTCCGGGCCAATAAGCCTGCCGTGGTAGGCGAACCGGACATGCCACATACCATCGCTGAGGTAGCGAAAGAGAAGGGCGCTCGTCTGCTGCGTCGCGGCGTGGACTGGCAGTATGAGGTGCAGGACAACGGCTGGCGCTTCAGCGATGCGCAGGGCGTGCTGGAGAGTCTGCCCCTGCCGCAGGTGCCACAGCCTAACGCGGCGACTGCGCTGGCAGCCCTGCGCGCCAGCGGATTAGCGGTAAGCGAGCAGGCAATCCGCGATGGCATTCAGAACGCACTGTTACCAGGACGTTTTCAGATTGTGAGTGAGTCACCGCGTCTGATTCTGGATGTTGCACATAACCCGCATGCGGCGGCGTACCTCGCAGGACGTCTCAAATCGTTACCAAAAACCGGGCGCGTGCTGGCGGTTATCGGTATGCTTCATGATAAAGATATCGGCGGCACGCTGGCCTGCATGGAGAGTGTGGTCGATAGCTGGTATTGTGCTCCTCTGGAAGGGCCGCGCGGCGCGACTGCTGAGCAGTTGATGGAACATCTCGGCAAAGGCGAAATCTACAGCAGTGTGGTTTCGGCCTGGCGTGCCGCGATGGCGGAGGCTAAACCAGAAGATACCGTGCTGGTGTGTGGGTCATTCCACACGGTGGCACATGTCATGGAAGTGATGGACGCGGGGAGAACCGGTGGCGAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,432 total letters Query= IDP95852.102 Length=1671 Score E Sequences producing significant alignments: (Bits) Value wt 2284 0.0 08E_E2_IDP95852.102_T7term.seq 1279 0.0 02E_E2_IDP95852.102_T7short.seq 1095 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 58 TTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGG 147 0 18 ..................................................................T.A-.................... 106 S H P Q F E K G T E N L Y F Q S N A M E N K S I P Q A T S P Query_1 148 TCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGGAAAATAAAAGCATTCCTCAAGCCACGTCGCCC 237 2 1 .................................... 36 0 107 .......................................................................................... 196 L A A W L S Y L E N L H S K T I D M G L E R V S Q V A A R L Query_1 238 CTGGCCGCGTGGCTTTCTTATCTGGAAAACCTGCACAGTAAAACTATCGACATGGGCCTTGAGCGCGTAAGCCAGGTTGCCGCGCGTCTT 327 2 37 .......................................................................................... 126 1 462 ........... 452 0 197 ......A...................G.................C..T..T.....G.....A.................T........C 286 D V L K P A P F V F T V A G T N G K G T T C R T L E S V L M Query_1 328 GACGTGCTGAAACCCGCGCCTTTCGTGTTTACCGTCGCCGGCACTAACGGTAAAGGCACCACCTGCCGCACGCTGGAATCTGTCCTGATG 417 2 127 .......................................................................................... 216 0 287 ..T...........T.....G..T..T..............A..C.......................T.........G........... 376 A A G Y K V G V Y S S P H L V R Y T E R V R V Q N T E L A E Query_1 418 GCGGCGGGGTACAAAGTTGGCGTTTACAGCTCGCCGCATCTGGTGCGCTATACCGAGCGCGTGCGGGTGCAAAACACCGAGCTGGCGGAA 507 2 217 .......................................................................................... 306 0 377 ..T..C..T.....G.....T..C........T.....C.....A..T...........G..T..C..........G...A...C.A..G 466 S A H T A S F A A I E A A R G E T S L T Y F E Y G T L S A Query_1 508 TCGGCCCATACGGCGTCGTTTGCCGCTATCGAA-GCGGCGCGCGGCGAAACCTCATTAACCTATTTCGAATATGGC-ACCCTGTCGGCGC 595 2 307 .................................-..........................................-............. 394 1 1094 ...T......C..G.AA..N...A.......N..NGN.G.TN......-.............C...N...........A. 1016 0 467 ..T..G..............C..G.AA..T...-...........T..G.T.....................C...-........N..A. 554 L W L F K Q A Q L D V V I L E V G L G G R L D A T N M V D Query_1 596 TGTGGCTGTTTAAGCAGGCGCAGCTGGA-CGTGGTGATCCTGGAAGTGGGGCT-GGGCGGGCGTCTGGATGCCA-CCAATATGGTGGATG 682 2 395 ............................-........................-....................-............... 481 1 1015 .......NG....A......N.-.....A.........T...........C..C..NN................N............... 927 0 555 .............A........A.....-.........T...........C..-C...................-............... 641 A D V A V V T S I A L D H T D W L G P D R E S I G R E K Query_1 683 CG-GATGTCGCCG-TCGTCACC--AGCATCGCGCTGGATCACACCGACTGGCTGGGCCCGGACCGCGAGA-GCATTGGCCGTGAGAA-GG 766 2 482 ..-..........-........--..............................................-................-.. 565 1 926 .CC.....G.G..G.G......CA..T..T..C........T.................C..T.....A.A.T..............A.. 837 0 642 .C-.....G..G.-.G......--..T..T..C........T.................C..T.....A.-.T..............-.. 725 A G I F R A N K P A V V G E P D M P H T I A E V A K E K G A Query_1 767 CCGGTATTTTCCGGGCCAATAAGCCTGCCGTGGTAGGCGAACCGGACATGCCACATACCATCGCTGAGGTAGCGAAAGAGAAGGGCGCTC 856 2 566 .......................................................................................... 655 1 836 .............CA.G........C..G.....T.....G.....T........C.....T..C..C..G................... 747 0 726 .............CA.G........C..GT....T.....G...N.T........C.....T..C..C..-.GNN............... 814 R L L R R G V D W Q Y E V Q D N G W R F S D A Q G V L E S L Query_1 857 GTCTGCTGCGTCGCGGCGTGGACTGGCAGTATGAGGTGCAGGACAACGGCTGGCGCTTCAGCGATGCGCAGGGCGTGCTGGAGAGTCTGC 946 2 656 .......................................................................................... 745 1 746 AC........C..G..T....................T...A...............ATC.............AAC........A..... 657 0 815 A.......N.C..G..T.........N..........T..-..TC..N......N..ATC..... 878 P L P Q V P Q P N A A T A L A A L R A S G L A V S E Q A I R Query_1 947 CCCTGCCGCAGGTGCCACAGCCTAACGCGGCGACTGCGCTGGCAGCCCTGCGCGCCAGCGGATTAGCGGTAAGCGAGCAGGCAATCCGCG 1036 2 746 .......................................................................................... 835 1 656 .............A..G..A.....T..C..C..C..C.....G..A........A..T..GC.G.....G.....A.....C....... 567 D G I Q N A L L P G R F Q I V S E S P R L I L D V A H N P H Query_1 1037 ATGGCATTCAGAACGCACTGTTACCAGGACGTTTTCAGATTGTGAGTGAGTCACCGCGTCTGATTCTGGATGTTGCACATAACCCGCATG 1126 2 836 .......................................................................................... 925 1 566 .C..T........T..GA.TC.G..C..G..............C..C..CG.T.....G.....A........C................ 477 A A A Y L A G R L K S L P K T G R V L A V I G M L H D K D I Query_1 1127 CGGCGGCGTACCTCGCAGGACGTCTCAAATCGTTACCAAAAACCGGGCGCGTGCTGGCGGTTATCGGTATGCTTCATGATAAAGATATCG 1216 2 926 .......................................................................................... 1015 1 476 .T........T.....C.CG...................................................................... 387 G G T L A C M E S V V D S W Y C A P L E G P R G A T A E Q L Query_1 1217 GCGGCACGCTGGCCTGCATGGAGAGTGTGGTCGATAGCTGGTATTGTGCTCCTCTGGAAGGGCCGCGCGGCGCGACTGCTGAGCAGTTGA 1306 2 1016 .......................................................................................... 1105 1 386 ....T.....................................................G...........T.....G..C........AC 297 M E H L G K G E I Y S S V V S A W R A A M A E A K P E D T V Query_1 1307 TGGAACATCTCGGCAAAGGCGAAATCTACAGCAGTGTGGTTTCGGCCTGGCGTGCCGCGATGGCGGAGGCTAAACCAGAAGATACCGTGC 1396 2 1106 .......................................................................................... 1195 1 296 .....................C........AT..C....C.CA...A....AA..............T...................... 207 L V C G S F H T V A H V M E V M D A G R T G G E * H W K W I Query_1 1397 TGGTGTGTGGGTCATTCCACACGGTGGCACATGTCATGGAAGTGATGGACGCGGGGAGAACCGGTGGCGAGTAACATTGGAAGTGGATAA 1486 2 1196 ....................................................................... 1266 1 206 ..........T..G............................................................................ 117 T D P N S S S V D K L A A A L E H H H H H H * D P A A N K Query_1 1487 CGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAA--CAAAGC 1574 1 116 ..................................................................................ACA..... 27 A R K E Query_1 1575 CCGAAAGGAA 1584 1 26 ......A... 17 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5600520 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 3,432 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95861.102 (IDP95861.1-267.F..pMCSG68)
MIDKSAVIHPSSIIEEGAVIGANVRIGPFCVIGSHVEIGEGTDIKSHVVINGHTRIGRDNQIYQFASIGEVNQDLKYRGEPTQVIIGDRNLIRESVTIHRGTTQGGNITKIGNDNLLMINTHVAHDCIIGDRCIIANNGTLGGHVTLGDYVIIGGMSAVHQFCQIGSHVMVGGCSGVAQDVPPFVIAQGNHATPYGLNIEGLKRRGFAKEDLHAIRNAYKILYRNGKTLEEAREEIAQLAADNNNQYVKIFSDFLENSAKSNRGIIR
ATGATTGATAAATCCGCAGTAATTCACCCTTCTTCAATTATTGAAGAGGGTGCGGTAATTGGTGCTAATGTTCGCATTGGCCCTTTTTGCGTTATTGGTAGCCATGTTGAGATTGGTGAAGGTACAGATATCAAATCTCATGTGGTTATTAATGGCCATACACGCATTGGCCGTGATAATCAGATTTACCAATTTGCCTCTATCGGTGAAGTAAACCAAGATCTGAAATATCGTGGTGAGCCGACCCAAGTCATTATTGGTGATCGCAATCTTATCCGCGAAAGTGTCACTATTCATCGTGGTACGACACAAGGCGGTAATATTACTAAAATTGGCAATGATAACTTATTGATGATCAACACCCATGTTGCTCATGATTGTATCATTGGCGATCGCTGTATTATTGCAAATAACGGTACTTTAGGTGGACATGTCACTTTAGGTGATTATGTGATAATCGGTGGTATGAGTGCGGTTCACCAATTTTGTCAGATTGGTTCACATGTCATGGTGGGCGGCTGTTCCGGTGTAGCACAAGATGTTCCTCCTTTTGTGATTGCACAAGGAAACCATGCCACCCCTTATGGTTTGAATATTGAAGGATTAAAACGTCGCGGTTTTGCAAAAGAAGATCTGCATGCCATTCGTAACGCTTACAAGATACTTTATCGTAATGGTAAAACGCTTGAAGAAGCGCGAGAAGAGATTGCTCAATTAGCCGCAGACAATAATAATCAGTATGTAAAAATATTCAGTGATTTTCTGGAAAACTCAGCAAAATCAAACCGCGGTATTATTCGC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,274 total letters Query= IDP95861.102 Length=1206 Score E Sequences producing significant alignments: (Bits) Value 09H_H3_IDP95861.102_T7term.seq 1893 0.0 03H_H3_IDP95861.102_T7short.seq 1772 0.0 wt 1445 0.0 E I N T T H Y R G I V S G * Q F P S R N N F V * L * E G D Query_1 13 GAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCT-CTAGAAATAATTTTGTTTAAC-TTTAAGAAGGAGATA 100 1 1131 .......T........C.NTANN................NN...........T........N..........N.N.........A..... 1042 1 1080 ...........NNT.C.C.C......... 1108 0 33 .....N............-............... 65 I H M H H H H H H S S G V D L W S H P Q F E K G T E N L Y Query_1 101 TACAT-ATGCA-CCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACC-GAGAACCTGTACT 187 1 1041 .....T.....N................................................................C............. 952 0 66 .....-.....-................................................................-............. 152 F Q S N A M I D K S A V I H P S S I I E E G A V I G A N V R Query_1 188 TCCAATCCAATGCCATGATTGATAAATCCGCAGTAATTCACCCTTCTTCAATTATTGAAGAGGGTGCGGTAATTGGTGCTAATGTTCGCA 277 1 951 .T.........................................................................-.............. 863 0 153 .......................................................................................... 242 2 1 ............................................................................ 76 I G P F C V I G S H V E I G E G T D I K S H V V I N G H T R Query_1 278 TTGGCCCTTTTTGCGTTATTGGTAGCCATGTTGAGATTGGTGAAGGTACAGATATCAAATCTCATGTGGTTATTAATGGCCATACACGCA 367 1 862 .......................................................................................... 773 0 243 .......................................................................................... 332 2 77 .......................................................................................... 166 I G R D N Q I Y Q F A S I G E V N Q D L K Y R G E P T Q V I Query_1 368 TTGGCCGTGATAATCAGATTTACCAATTTGCCTCTATCGGTGAAGTAAACCAAGATCTGAAATATCGTGGTGAGCCGACCCAAGTCATTA 457 1 772 .......................................................................................... 683 0 333 .......................................................................................... 422 2 167 .......................................................................................... 256 I G D R N L I R E S V T I H R G T T Q G G N I T K I G N D N Query_1 458 TTGGTGATCGCAATCTTATCCGCGAAAGTGTCACTATTCATCGTGGTACGACACAAGGCGGTAATATTACTAAAATTGGCAATGATAACT 547 1 682 .......................................................................................... 593 0 423 .......................................................................................... 512 2 257 .......................................................................................... 346 L L M I N T H V A H D C I I G D R C I I A N N G T L G G H V Query_1 548 TATTGATGATCAACACCCATGTTGCTCATGATTGTATCATTGGCGATCGCTGTATTATTGCAAATAACGGTACTTTAGGTGGACATGTCA 637 1 592 .......................................................................................... 503 0 513 .......................................................................................... 602 2 347 .......................................................................................... 436 T L G D Y V I I G G M S A V H Q F C Q I G S H V M V G G C S Query_1 638 CTTTAGGTGATTATGTGATAATCGGTGGTATGAGTGCGGTTCACCAATTTTGTCAGATTGGTTCACATGTCATGGTGGGCGGCTGTTCCG 727 1 502 .......................................................................................... 413 0 603 .......................................................................................... 692 2 437 .......................................................................................... 526 G V A Q D V P P F V I A Q G N H A T P Y G L N I E G L K R R Query_1 728 GTGTAGCACAAGATGTTCCTCCTTTTGTGATTGCACAAGGAAACCATGCCACCCCTTATGGTTTGAATA-TTGAAGGATTAAAACGTCGC 816 1 412 .....................................................................-.................... 324 0 693 .....................................................................T.................... 782 2 527 .....................................................................-.................... 615 G F A K E D L H A I R N A Y K I L Y R N G K T L E E A R E E Query_1 817 GGTTTTGCAAAAGAAGATCTGCATGCCATTCGTAACGCTTACAAGATACTTTATCGTAATGGTAAAACGCTTGAAGAAGCGCGAGAAGAG 906 1 323 .......................................................................................... 234 0 783 ........-.................................-............................................... 870 2 616 .......................................................................................... 705 I A Q L A A D N N N Q Y V K I F S D F L E N S A K S N R G I Query_1 907 ATTGCTCAATTAGCCGCAGACAATAATAATCAGTATGTAAAAATATTCAGTGATTTTCTGGAAAACTCAGCAAAATCAAACCGCGGTATT 996 1 233 .......................................................................................... 144 0 871 ..........--AN..................-N...........................-.......--C.....-N....T...... 953 2 706 .......................................................................................... 795 I R * H W K W I T D P N S S S V D K L A A A L E H H H H H Query_1 997 ATTCGCTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCA-CTCGAGcaccaccaccaccacca 1085 1 143 ..................................................................-....................... 55 0 954 ...................-.......................-....N.........NC.....NG...........-...T...NN.. 1040 2 796 ...... 801 H * D P A A N K A R K E A E L A A A T A E Q * L A * P L G Query_1 1086 cTGAGATCCGGCTGCTAACAAAGCCC---GAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGC 1172 1 54 ..........................CNGA........ 17 0 1041 N...A..............-......---.-....NGCT...T............T.......NN.--T.............T.G..A.. 1123 A S K R V L R G F L Query_1 1173 -CTCTAAACGGGTC-TTGAG-GGGTTTTTTGCT 1202 0 1124 TN.....C......T.....T..A......... 1156 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3838940 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 3,274 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95866.102 (IDP95866.1-307.F..pMCSG68)
MSMADRDGVIWYDGELVQWRDATTHVLTHTLHYGMGVFEGVRAYDTPQGTAIFRLQAHTDRLFDSAHIMNMQIPYSRDEINEATAPPCARTTWKAPISARWCSTGSEGMGLRASGLKVHVIIASWSWGAYMGEEALQQGIKVRTSSFTRHHVNISMTRAKSNGAYINSMLALQEAISGGADEAMMLDPEGYVAEGSGENIFIIKDGVIYTPEVTACLNGITRNTILTLAAEHGFKLVEKRITRDEVYIADEAFFTGTAAEVTPIREVDGRKIGAGRRGPVTEKLQKAYFDLVSGKTEAHAEWRTLVK
ATGTCGATGGCCGATCGTGATGGCGTGATCTGGTATGACGGTGAACTGGTGCAGTGGCGCGACGCGACCACGCACGTGCTGACCCATACCCTGCACTATGGAATGGGCGTGTTCGAGGGCGTGCGCGCCTACGACACCCCGCAGGGCACGGCGATCTTCCGCCTGCAGGCGCATACCGACCGGCTGTTCGACTCCGCGCACATCATGAACATGCAGATCCCGTACAGCCGCGACGAGATCAACGAGGCGACCGCGCCGCCGTGCGCGAGAACAACCTGGAAAGCGCCTATATCCGCCCGATGGTGTTCTACGGGAAGCGAAGGCATGGGCCTGCGCGCCAGCGGCCTGAAGGTCCATGTGATCATCGCCTCCTGGAGCTGGGGCGCCTACATGGGCGAGGAAGCCCTGCAGCAAGGCATCAAGGTGCGCACCAGTTCCTTCACCCGCCACCACGTCAACATCTCGATGACCCGTGCCAAGTCCAACGGCGCCTACATCAACTCGATGCTGGCCCTCCAGGAAGCGATCTCCGGCGGCGCCGACGAGGCCATGATGCTCGATCCGGAAGGCTACGTGGCCGAAGGCTCCGGCGAGAACATCTTCATCATCAAGGATGGCGTGATCTACACCCCGGAAGTCACCGCCTGCCTGAACGGCATCACTCGTAACACTATCCTGACCCTGGCCGCCGAACACGGTTTTAAACTGGTCGAGAAGCGCATCACCCGCGACGAGGTGTACATCGCCGACGAGGCCTTCTTCACTGGCACTGCCGCGGAAGTCACGCCGATCCGCGAAGTGGACGGTCGCAAGATCGGCGCCGGCCGCCGTGGCCCGGTCACCGAAAAGCTGCAGAAAGCCTATTTCGACCTGGTCAGCGGCAAGACCGAGGCCCACGCCGAGTGGCGTACCCTGGTCAAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,736 total letters Query= IDP95866.102 Length=1326 Score E Sequences producing significant alignments: (Bits) Value wt 1662 0.0 04A_A4_IDP95866.102_T7short.seq 1517 0.0 10A_A4_IDP95866.102_T7term.seq 1388 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 60 CCCCTCT-AGAAATAATTTT--GTTT--AACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTG 144 0 22 .......T............TT....TA.............................................................. 111 W S H P Q F E K G T E N L Y F Q S N A M S M A D R D G V I W Query_1 145 TGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGTCGATGGCCGATCGTGATGGCGTGATCTGG 234 2 1 ................................. 33 2 613 ............. 625 0 112 .......................................................................................... 201 0 780 ............. 792 1 445 ............. 433 Y D G E L V Q W R D A T T H V L T H T L H Y G M G V F E G V Query_1 235 TATGACGGTGAACTGGTGCAGTGGCGCGACGCGACCACGCACGTGCTGACCCATACCCTGCACTATGGAATGGGCGTGTTCGAGGGCGTG 324 2 34 .......................................................................................... 123 0 202 .........................................................A................................ 291 R A Y D T P Q G T A I F R L Q A H T D R L F D S A H I M N M Query_1 325 CGCGCCTACGACACCCCGCAGGGCACGGCGATCTTCCGCCTGCAGGCGCATACCGACCGGCTGTTCGACTCCGCGCACATCATGAACATG 414 2 124 .......................................................................................... 213 2 171 ............ 160 0 292 .......................................................................................... 381 0 339 ............ 328 Q I P Y S R D E I N E A T A P P C A R T T W K A P I S Query_1 415 CAGATCCCGTACA-GCCGCGACGAGATCAACGAGG-CGA-CC--GCGCCGCCGTGCG-CGAGAACAA-CCTGGAAAGCGCCTATATCCGC 497 2 214 .............-.....................-...-..--.............-.........-...................... 296 0 382 .............-.....................-...C..--.............-......T..-...................... 465 1 848 ............A.....................G..G-..CC.............N......T..A................TC.... 761 A R W C S T G S E G M G L R A S G L K V H V I I A S W S W G Query_1 498 CCGATGGTGTTCTACGGGAAGCGAAGGCATGGGCCTGCGCGCCAGCGGCCTGAAGGTCCATGTGATCATCGCCTCCTGGAGCTGGGGCGC 587 2 297 .......................................................................................... 386 2 487 ..... 491 0 466 ...............-.........N.N.............................................G................ 554 0 655 ..... 659 1 760 .................-.......................................................G................ 672 1 571 ..... 567 A Y M G E E A L Q Q G I K V R T S S F T R H H V N I S M T R Query_1 588 CTACATGGGCGAGGAAGCCCTGCAGCAAGGCATCAAGGTGCGCACCAGTTCCTTCACCCGCCACCACGTCAACATCTCGATGACCCGTGC 677 2 387 .......................................................................................... 476 2 492 ...... 497 0 555 .................................................................N.....................C.. 644 0 660 ...... 665 1 671 .......................................................................................C.. 582 1 566 ...... 561 A K S N G A Y I N S M L A L Q E A I S G G A D E A M M L D P Query_1 678 CAAGTCCAACGGCGCCTACATCAACTCGATGCTGGCCCTCCAGGAAGCGATCTCCGGCGGCGCCGACGAGGCCATGATGCTCGATCCGGA 767 2 477 .......................................................................................... 566 2 744 ............. 756 2 382 ........... 392 0 645 ...........................N..............N.................N............................. 734 0 550 ........... 560 0 915 .......... 924 1 581 .......................................................................................... 492 1 314 ............. 302 1 676 ........... 666 E G Y V A E G S G E N I F I I K D G V I Y T P E V T A C L N Query_1 768 AGGCTACGTGGCCGAAGGCTCCGGCGAGAACATCTTCATCATCAAGGATGGCGTGATC-TACACCCCGGAAGTCACCGCCTGCCTGAA-C 855 2 567 ..........................................................-.............................-. 654 2 19 ............-. 31 0 735 .-................N.......................................T.........N...................N. 823 0 187 ............-. 199 1 491 ...N......................................................-.............................-. 404 G I T R N T I L T L A A E H G F K L V E K R I T R D E V Y I Query_1 856 GGCATCACTCGTAACACTATCCTGACCCTGGCCGCCGAACACGGTTTTAAACTGGTCGAGAAGCGCATCACCCGCGACGAGGTGTACATC 945 2 655 .......................................................................................... 744 2 537 . 537 0 824 ...............N...............A................-......N.................N...............N 912 1 403 .......................................................................................... 314 1 521 . 521 A D E A F F T G T A A E V T P I R E V D G R K I G A G R R Query_1 946 GCCGACGAGGCCTTCTTCACTGGCACTGCCGCGGAA-GTCACGCCGATCCGCGAAGTGGACGGTCGCAAGATCGGCGCCGGCCGCCGTGG 1034 2 745 ....................................-..................................................... 833 2 538 ............ 549 0 913 CN.................N...N.....N......T.....N.. 957 0 706 ............ 717 1 313 ....................................-..................................................... 225 1 520 ............ 509 G P V T E K L Q K A Y F D L V S G K T E A H A E W R T L V K Query_1 1035 CCCGGTCACCGAAAAGCTGCAGAAAGCCTATTTCGACCTGGTCAGCGGCAAGACCGAGGCCCACGCCGAGTGGCGTACCCTGGTCAAGTA 1124 2 834 ........................................................................................ 921 1 224 .......................................................................................... 135 * H W K W I T D P N S S S V D K L A A A L E H H H H H H * D Query_1 1125 ACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTG-AGATC 1213 1 134 ....................................................................................A..... 45 P A A N K A R Query_1 1214 CGGCTGCTAACAAA--GCCCGAAA 1235 1 44 ..............AGC....... 21 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3521280 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 2,736 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95886.102 (IDP95886.1-159.F..pMCSG68)
MTLAYIAIGSNLASPLEQVNAAVQALGEIPQSKIVAVSSFYRTPPLGPQDQPDYLNAAVILETELGPEELLDNTQRIELQQGRVRKAERWGPRTLDLDIMLFGHETINTERLTVPHYDMKNRGFMLWPLFEVAPDLIFPDGTPLKSILDNLNAERPARW
ATGACCCTTGCGTATATCGCCATCGGCAGCAATTTAGCCTCTCCGCTGGAGCAGGTGAATGCTGCCGTACAGGCGCTGGGTGAGATCCCGCAAAGCAAGATTGTGGCTGTCTCCTCGTTCTACCGCACTCCGCCGTTGGGTCCGCAGGATCAGCCTGATTATCTGAACGCCGCCGTGATCCTTGAGACTGAACTGGGCCCAGAAGAGCTGCTGGACAATACCCAGCGCATCGAACTGCAGCAGGGCCGCGTTCGCAAAGCCGAGCGCTGGGGACCGCGCACGCTGGACCTCGACATTATGCTGTTTGGTCACGAGACCATTAACACTGAACGTCTCACCGTTCCGCATTACGACATGAAAAATCGCGGGTTTATGCTCTGGCCGCTGTTTGAAGTCGCCCCCGATCTTATCTTCCCGGATGGCACCCCACTTAAGTCCATTCTGGACAACCTCAACGCGGAAAGACCCGCTCGCTGG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,985 total letters Query= IDP95886.102 Length=882 Score E Sequences producing significant alignments: (Bits) Value 02F_F2_IDP95886.102_T7short.seq 1148 0.0 08F_F2_IDP95886.102_T7term.seq 1081 0.0 wt 861 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAA 90 0 16 ..............-................... 48 1 816 .......................................................................................... 727 1 749 ...G...........T.T.C.C.C...........A... 787 E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E N Query_1 91 GAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAAC 180 0 49 .......................................................................................... 138 1 726 .......................................................................................... 637 L Y F Q S N A M T L A Y I A I G S N L A S P L E Q V N A A V Query_1 181 CTGTACTTCCAATCCAATGCCATGACCCTTGCGTATATCGCCATCGGCAGCAATTTAGCCTCTCCGCTGGAGCAGGTGAATGCTGCCGTA 270 0 139 ......................................................C.G........T...........T...........T 228 1 636 ......................................................C.G........T...........T...........T 547 2 1 ..................................................................... 69 Q A L G E I P Q S K I V A V S S F Y R T P P L G P Q D Q P D Query_1 271 CAGGCGCTGGGTGAGATCCCGCAAAGCAAGATTGTGGCTGTCTCCTCGTTCTACCGCACTCCGCCGTTGGGTCCGCAGGATCAGCCTGAT 360 0 229 ....................C..G...CGC........G...........T.....T..A......C.C..C.................. 318 1 546 ....................C..G...CGC........G...........T.....T..A......C.C..C.................. 457 2 70 .......................................................................................... 159 Y L N A A V I L E T E L G P E E L L D N T Q R I E L Q Q G R Query_1 361 TATCTGAACGCCGCCGTGATCCTTGAGACTGAACTGGGCCCAGAAGAGCTGCTGGACAATACCCAGCGCATCGAACTGCAGCAGGGCCGC 450 0 319 ..C.....T.........G.G..G..C..C.CG....ATG.C...AC.T....T..T..C...........T..G.....A.....T... 408 1 456 ..C.....T.........G.G..G..C..C.CG....ATG.C...AC.T....T..T..C...........T..G.....A.....T... 367 2 160 .......................................................................................... 249 V R K A E R W G P R T L D L D I M L F G H E T I N T E R L T Query_1 451 GTTCGCAAAGCCGAGCGCTGGGGACCGCGCACGCTGGACCTCGACATTATGCTGTTTGGTCACGAGACCATTAACACTGAACGTCTCACC 540 0 409 ..G..T.....A...........G..A..T..C..T...........C...........CA.....GTG.........TC...C..G..G 498 1 366 ..G..T.....A...........G..A..T..C..T...........C...........CA.....GTG.........TC...C..G..G 277 2 250 .......................................................................................... 339 V P H Y D M K N R G F M L W P L F E V A P D L I F P D G T P Query_1 541 GTTCCGCATTACGACATGAAAAATCGCGGGTTTATGCTCTGGCCGCTGTTTGAAGTCGCCCCCGATCTTATCTTCCCGGATGGCACCCCA 630 0 499 ..G..........................A......................................C.C.........C....T...G 588 1 276 ..G..........................A......................................C.C.........C....T...G 187 2 340 .......................................................................................... 429 L K S I L D N L N A E R P A R W * H W K W I T D P N S S S V Query_1 631 CTTAAGTCCATTCTGGACAACCTCAACGCGGAAAGACCCGCTCGCTGGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTC 720 0 589 ..C.G.GAG..C............C....A............................................................ 678 1 186 ..C.G.GAG..C............C....A............................................................ 97 2 430 ................................................ 477 D K L A A A L E H H H H H H * D P A A N K A R K E A E L Query_1 721 GACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAAC-AAAGCCC---GAAAGGAAGCTGAGTTGGC 806 0 679 ............................................................-.......---................... 764 1 96 ............................................................A.......CGAA........ 17 A A A T A E Q * L A * P L G A S K R V L R G F L L K Query_1 807 TGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAA 882 0 765 ............................................................................ 840 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2548980 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 2,985 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95890.102 (IDP95890.1-315.F..pMCSG68)
MSNNNDYDISDSKDCEKFANQFIQEFPIRSAEIGQGTVIKRALPSRQKRMIGAWCFLDHAGPVTFPAGNGLDVGPHPHIGLQTFTWMIEGTMMHTDSLGSKQLIRPKQVNLMTAGHGISHTEVAPDTETQMHAAQLWIALPDHKRNMDPKFEHYPDLPVVEKDGLEFTVLVGEYLETTSPVVVHTPLVGVDLIATQDTKTRIPLNPEFEYGFMALDGVAHVNGHELTADNMVVLDTGLNEIEIEVKKGNRVLLIGGEPFETPILLWWNFVARTMDDLKEAREQWVNHDVRFGEIPDYVGARLEAPVLPDQMRASK
ATGAGCAATAATAACGATTACGATATTTCGGATTCAAAGGATTGCGAAAAATTTGCCAATCAATTTATTCAGGAATTTCCAATTCGCTCTGCCGAAATTGGACAGGGGACGGTGATTAAGCGGGCTTTACCTAGTCGACAAAAAAGAATGATTGGGGCATGGTGCTTTTTAGATCATGCAGGACCAGTAACATTTCCTGCCGGCAATGGTTTAGATGTAGGACCTCATCCACATATTGGTTTGCAAACCTTCACTTGGATGATTGAAGGGACCATGATGCATACCGATAGTTTAGGGTCAAAGCAATTAATTCGCCCTAAACAGGTAAACTTGATGACAGCAGGACATGGTATTTCTCATACCGAGGTTGCACCTGATACTGAAACTCAAATGCATGCGGCACAGCTCTGGATTGCATTGCCGGACCATAAGCGCAACATGGATCCGAAATTTGAACATTATCCCGATCTTCCTGTTGTTGAAAAAGACGGCCTGGAATTTACTGTACTGGTGGGTGAATATTTAGAGACGACTTCTCCAGTTGTGGTTCATACACCGTTAGTAGGGGTTGATCTCATTGCTACCCAAGATACTAAGACACGTATTCCACTAAATCCTGAATTTGAATATGGTTTTATGGCATTAGATGGCGTAGCTCATGTGAATGGCCATGAACTTACTGCTGACAATATGGTTGTATTGGATACAGGTTTGAACGAAATTGAGATTGAAGTAAAGAAAGGGAACCGTGTGCTATTAATCGGTGGTGAACCTTTTGAAACTCCGATCTTATTATGGTGGAATTTCGTTGCACGTACGATGGACGATTTAAAAGAAGCACGTGAGCAATGGGTTAATCATGACGTGCGTTTTGGTGAAATTCCAGATTATGTGGGTGCCCGCTTAGAAGCACCAGTTCTTCCAGATCAAATGAGAGCATCAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,433 total letters Query= IDP95890.102 Length=1350 Score E Sequences producing significant alignments: (Bits) Value wt 1705 0.0 07A_A1_IDP95890.102_T7term.seq 1314 0.0 01A_A1_IDP95890.102_T7short.seq 1196 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 58 TTCCCCT-CTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTG 146 0 18 .....N.N...-..............N......................................N..N..................... 106 W S H P Q F E K G T E N L Y F Q S N A M S N N N D Y D I S D Query_1 147 GTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGAGCAATAATAACGATTACGATATTTCGGATTC 236 2 1 ................................... 35 0 107 .......................................................N.................................. 196 S K D C E K F A N Q F I Q E F P I R S A E I G Q G T V I K R Query_1 237 AAAGGATTGCGAAAAATTTGCCAATCAATTTATTCAGGAATTTCCAATTCGCTCTGCCGAAATTGGACAGGGGACGGTGATTAAGCGGGC 326 2 36 .......................................................................................... 125 0 197 .......................................................................................... 286 A L P S R Q K R M I G A W C F L D H A G P V T F P A G N G L Query_1 327 TTTACCTAGTCGACAAAAAAGAATGATTGGGGCATGGTGCTTTTTAGATCATGCAGGACCAGTAACATTTCCTGCCGGCAATGGTTTAGA 416 2 126 .......................................................................................... 215 0 287 .......................................................N.......................G.......... 376 D V G P H P H I G L Q T F T W M I E G T M M H T D S L G S K Query_1 417 TGTAGGACCTCATCCACATATTGGTTTGCAAACCTTCACTTGGATGATTGAAGGGACCATGATGCATACCGATAGTTTAGGGTCAAAGCA 506 2 216 .......................................................................................... 305 2 262 .......AG.GAA............AAT.TG....... 225 0 377 ...................................................................N...................... 466 0 423 .......AG.GAA............AAT.TG....... 386 Q L I R P K Q V N L M T A G H G I S H T E V A P D T E T Q M Query_1 507 ATTAATTCGCCCTAAACAGGTAAACTTGATGACAGCAGGACATGG-TATTTCTCATACCGAGGTTGCACCTGATACTGAAACTCAAATGC 595 2 306 .............................................-............................................ 394 1 762 ................................N............................................ 686 0 467 .............................................-.....................N...................... 555 H A A Q L W I A L P D H K R N M D P K F E H Y P D L P V V E Query_1 596 ATGCGGCACAGCTCTGGATTGCATTGCCGGACCATAAGCGCAACATGGATCCGAAATTTGAACATTATCCCGATCTTCCTGTTGTTGAAA 685 2 395 .......................................................................................... 484 1 685 .......................................................................................... 596 0 556 .....................N.................................................N.................. 645 K D G L E F T V L V G E Y L E T T S P V V V H T P L V G V D Query_1 686 AAGACGGCCTGGAATTTACTGTACTGGTGGGTGAATATTTAGAGACGACTTCT-CCAGTTGTGGTTCATACACCGTTAGTAGGGGTTGAT 774 2 485 .....................................................-.................................... 573 1 595 .....................................................-.................................... 507 0 646 .....................................-...............C................T...... 721 L I A T Q D T K T R I P L N P E F E Y G F M A L D G V A H V Query_1 775 CTCATTGCTACCCAAGATACTAAGACACGTATTCCACTAAATCCTGAATTTGAATATGGTTTTATGGCATTAGATGGCGTAGCTCATGTG 864 2 574 .......................................................................................... 663 1 506 .......................................................................................... 417 N G H E L T A D N M V V L D T G L N E I E I E V K K G N R V Query_1 865 AATGGCCATGAACTTACTGCTGACAATATGGTTGTATTGGATACAGGTTTGAACGAAATTGAGATTGAAGTAAAGAAAGGGAACCGTGTG 954 2 664 .......................................................................................... 753 1 416 .......................................................................................... 327 L L I G G E P F E T P I L L W W N F V A R T M D D L K E A R Query_1 955 CTATTAATCGGTGGTGAACCTTTTGAAACTCCGATCTTATTATGGTGGAATTTCGTTGCACGTACGATGGACGATTTAAAAGAAGCACGT 1044 2 754 .......................................................................................... 843 1 326 .......................................................................................... 237 E Q W V N H D V R F G E I P D Y V G A R L E A P V L P D Q M Query_1 1045 GAGCAATGGGTTAATCATGACGTGCGTTTTGGTGAAATTCCAGATTATGTGGGTGCCCGCTTAGAAGCACCAGTTCTTCCAGATCAAATG 1134 2 844 .......................................................................................... 933 1 236 .......................................................................................... 147 R A S K * H W K W I T D P N S S S V D K L A A A L E H H H H Query_1 1135 AGAGCATCAAAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccac 1224 2 934 ............ 945 1 146 ...........................................................ANN..N......................... 57 H H * D P A A N K A R K E Query_1 1225 caccacTGA-GATCCGGCTGCTAACAAAGCCCGAAAGGAA 1263 1 56 .........N.............................. 17 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3181590 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 2,433 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95890.103 (IDP95890.1-315.F..pMCSG73)
MSNNNDYDISDSKDCEKFANQFIQEFPIRSAEIGQGTVIKRALPSRQKRMIGAWCFLDHAGPVTFPAGNGLDVGPHPHIGLQTFTWMIEGTMMHTDSLGSKQLIRPKQVNLMTAGHGISHTEVAPDTETQMHAAQLWIALPDHKRNMDPKFEHYPDLPVVEKDGLEFTVLVGEYLETTSPVVVHTPLVGVDLIATQDTKTRIPLNPEFEYGFMALDGVAHVNGHELTADNMVVLDTGLNEIEIEVKKGNRVLLIGGEPFETPILLWWNFVARTMDDLKEAREQWVNHDVRFGEIPDYVGARLEAPVLPDQMRASK
ATGAGCAATAATAACGATTACGATATTTCGGATTCAAAGGATTGCGAAAAATTTGCCAATCAATTTATTCAGGAATTTCCAATTCGCTCTGCCGAAATTGGACAGGGGACGGTGATTAAGCGGGCTTTACCTAGTCGACAAAAAAGAATGATTGGGGCATGGTGCTTTTTAGATCATGCAGGACCAGTAACATTTCCTGCCGGCAATGGTTTAGATGTAGGACCTCATCCACATATTGGTTTGCAAACCTTCACTTGGATGATTGAAGGGACCATGATGCATACCGATAGTTTAGGGTCAAAGCAATTAATTCGCCCTAAACAGGTAAACTTGATGACAGCAGGACATGGTATTTCTCATACCGAGGTTGCACCTGATACTGAAACTCAAATGCATGCGGCACAGCTCTGGATTGCATTGCCGGACCATAAGCGCAACATGGATCCGAAATTTGAACATTATCCCGATCTTCCTGTTGTTGAAAAAGACGGCCTGGAATTTACTGTACTGGTGGGTGAATATTTAGAGACGACTTCTCCAGTTGTGGTTCATACACCGTTAGTAGGGGTTGATCTCATTGCTACCCAAGATACTAAGACACGTATTCCACTAAATCCTGAATTTGAATATGGTTTTATGGCATTAGATGGCGTAGCTCATGTGAATGGCCATGAACTTACTGCTGACAATATGGTTGTATTGGATACAGGTTTGAACGAAATTGAGATTGAAGTAAAGAAAGGGAACCGTGTGCTATTAATCGGTGGTGAACCTTTTGAAACTCCGATCTTATTATGGTGGAATTTCGTTGCACGTACGATGGACGATTTAAAAGAAGCACGTGAGCAATGGGTTAATCATGACGTGCGTTTTGGTGAAATTCCAGATTATGTGGGTGCCCGCTTAGAAGCACCAGTTCTTCCAGATCAAATGAGAGCATCAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,697 total letters Query= IDP95890.103 Length=1350 Score E Sequences producing significant alignments: (Bits) Value wt 1705 0.0 12H_H6_IDP95890.103_T7_term.seq 1620 0.0 06H_H6_IDP95890.103_v73F.seq 958 0.0 L W S H P Q F E K G T E N L Y F Q S N A M S N N N D Y D I Query_1 141 TCTGTGGTCT-CATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGAGCAATAATAACGATTACGATATTT 229 2 1 ............................ 28 1 1037 ..........................-......C......-.....T....N... 985 0 35 ...C......T.....A-.......G..N.------.N.............T..T....................-.............. 116 S D S K D C E K F A N Q F I Q E F P I R S A E I G Q G T V I Query_1 230 CGGATTCAAA-GGATTGCGAAAAATTTGCCAATCAATTTATTCAGGAATTTCCAATTCGCTCTGCCGAAATTGGACAGGGGACGGTGATT 318 2 29 ..........-............................................................................... 117 1 984 ..........A.N.............N....-......N.A....-...........................N-.............N. 898 0 117 .........--........................N........N............................................. 204 K R A L P S R Q K R M I G A W C F L D H A G P V T F P A G Query_1 319 AAGCGGGCTTTACCTAGTCGACAAAAAAG-AATGATTGGGGCATGGTGCTTTTTAGATCATGCA-GGACCAGT-AACATTTCCTGCCGGC 405 2 118 .............................-..................................-........-................ 204 1 897 ..N........-......T..A......NN..................................N......A.N................ 809 0 205 ..N................N.....T..N-.......C.T........................-...T....-................ 291 N G L D V G P H P H I G L Q T F T W M I E G T M M H T D S L Query_1 406 AATGGTTTAGATGTAGGACCTCATCCACATATTGGTTTGCAAACCTTCACTTGGATGATTGAAGGGACCATGATGCATACCGATAGTTTA 495 2 205 .......................................................................................... 294 2 262 .......AG.GAA............AAT.TG....... 225 1 808 .......N................N................................................................. 719 1 751 .......AG.GAA............ 775 0 292 .........N............T...........N...........................N........................... 381 G S K Q L I R P K Q V N L M T A G H G I S H T E V A P D T Query_1 496 GGGTCAAA-GCAATTAATTCGCCCTAAACAGGTAAACTTGATGACAGCAGGACATGGTATTTCTCATACCGAGGTTGCACCTGATACTGA 584 2 295 ........-................................................................................. 383 1 718 ........A................N................................................................ 629 0 382 ...C....-......N....N...........N...N.......TT............................................ 470 E T Q M H A A Q L W I A L P D H K R N M D P K F E H Y P D L Query_1 585 AACTCAAATGCATGCGGCACAGCTCTGGA-TTGCATTGCCGGACCATAAGCGCAACATGGATCCGAAATTTGAACATTATCCCGATCTTC 673 2 384 .............................-............................................................ 472 1 628 .............................-............................................................ 540 0 471 .................N...NT......T...................N........................................ 560 P V V E K D G L E F T V L V G E Y L E T T S P V V V H T P L Query_1 674 CTGTTGTTGAAAAAGACGGCCTGGAATTTACTGTACTGGTGGGTGAATATTTAGAGACGACTTCTCCAGTTGTGGTTCATACACCGTTAG 763 2 473 .......................................................................................... 562 1 539 ......................................N................................................... 450 0 561 ........N..............N...................N.......................N...................... 650 V G V D L I A T Q D T K T R I P L N P E F E Y G F M A L D Query_1 764 TAGGGGTTGATCTCA-TTGCTA-CCCAAGATACTAAGACACGTATTCCACTAAATCCTGAATTTGAATATGGTTTTATGGCATTAGATGG 851 2 563 ...............-......-................................................................... 650 1 449 ...............-......-................................................................... 362 0 651 ............N..T......C........ 681 G V A H V N G H E L T A D N M V V L D T G L N E I E I E V K Query_1 852 CGTAGCTCATGTGAATGGCCATGAACTTACTGCTGACAATATGGTTGTATTGGATACAGGTTTGAACGAAATTGAGATTGAAGTAAAGAA 941 2 651 .......................................................................................... 740 1 361 ..................................................................N....................... 272 K G N R V L L I G G E P F E T P I L L W W N F V A R T M D D Query_1 942 AGGGAACCGTGTGCTATTAATCGGTGGTGAACCTTTTGAAACTCCGATCTTATTATGGTGGAATTTCGTTGCACGTACGATGGACGATTT 1031 2 741 .......................................................................................... 830 1 271 .......................................................................................... 182 L K E A R E Q W V N H D V R F G E I P D Y V G A R L E A P V Query_1 1032 AAAAGAAGCACGTGAGCAATGGGTTAATCATGACGTGCGTTTTGGTGAAATTCCAGATTATGTGGGTGCCCGCTTAGAAGCACCAGTTCT 1121 2 831 .......................................................................................... 920 1 181 ........................................................................................N. 92 L P D Q M R A S K * H W K W I T D P N S S S V D K L A A A L Query_1 1122 TCCAGATCAAATGAGAGCATCAAAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGA 1211 2 921 ......................... 945 1 91 ..............................-............N.--..... 43 E H H H H H H * D P A A N K A R K E Query_1 1212 GcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAG 1264 1 47 .........G-...............A... 19 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3533766 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 2,697 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95891.102 (IDP95891.1-286.F..pMCSG68)
MKSLAFIHRNDTRFAVGDFYPVLSVFSYHELGNTLSPFLLLDHSGPGKIAPSMKRRGVNDHPHRGFETVTLVYAGELEHKDSSGGGGFISAGDVQWMTAASGVIHRELFSEEYSRSGGPFEMIQLWVNLPAANKMNSPRYQSLKKAEIPMVKLENEAGFVRIIAGQFNHVIGPALTHTPITVLDVELHAGQQAAFPAQSGETALVYLRSGRAQFQKDEDVLEEQGLAVMSNQGEHFSITALQSCKLLILTGQPITEPINGHGPFVMNTYDEILQAYDDIKNGRFAK
ATGAAAAGTCTGGCTTTTATTCATCGTAACGATACTCGCTTTGCGGTCGGTGATTTTTATCCAGTTCTTTCTGTTTTTTCCTACCATGAGTTAGGCAATACCCTCTCACCCTTCTTACTACTCGACCATTCAGGTCCCGGAAAAATTGCACCTTCTATGAAACGTAGAGGAGTTAATGATCATCCCCATCGTGGATTCGAAACGGTAACACTTGTTTATGCAGGTGAACTAGAACACAAAGATTCATCTGGTGGAGGTGGATTTATAAGTGCTGGTGATGTGCAATGGATGACCGCAGCCTCTGGTGTGATTCATCGGGAATTGTTTAGTGAAGAGTATTCTCGATCTGGTGGTCCTTTTGAAATGATTCAACTTTGGGTCAATTTACCAGCAGCAAATAAAATGAACTCTCCGCGCTATCAAAGTTTAAAGAAAGCTGAAATACCGATGGTGAAACTGGAAAATGAGGCTGGATTTGTACGTATTATTGCAGGGCAGTTTAACCATGTTATAGGTCCTGCACTTACTCACACTCCTATTACCGTATTAGATGTAGAGCTCCATGCAGGTCAACAAGCCGCCTTCCCTGCTCAATCTGGTGAAACAGCTTTAGTTTATTTACGTTCAGGTCGGGCACAATTTCAAAAAGATGAAGACGTTTTAGAGGAACAAGGCCTAGCAGTCATGTCAAACCAAGGAGAGCATTTCTCTATTACTGCCCTGCAAAGTTGTAAATTACTTATTTTGACAGGACAACCCATCACAGAACCGATTAATGGTCATGGTCCATTTGTAATGAATACTTATGATGAGATTTTACAAGCCTATGATGATATTAAAAATGGAAGATTTGCCAAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,615 total letters Query= IDP95891.102 Length=1263 Score E Sequences producing significant alignments: (Bits) Value 07B_B1_IDP95891.102_T7term.seq 1651 0.0 wt 1548 0.0 01B_B1_IDP95891.102_T7short.seq 1232 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 58 TTCCCCTCTAGAAATAATTTTGTTTAACTTT-AAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTG 146 0 19 .............TA.T..............T.......................................................... 108 W S H P Q F E K G T E N L Y F Q S N A M K S L A F I H R N D Query_1 147 GTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAA-TGCCATGAAAAGTCTGGCTTTTATTCATCGTAACGATA 235 1 1028 ...A..NN...........-.N.......A...A............................T...-.A... 959 2 1 .................................. 34 0 109 ...................................................-.................N.................... 197 T R F A V G D F Y P V L S V F S Y H E L G N T L S P F L L Query_1 236 CTCGCTTTGCGGTCGGTGATTTTTATCCA-GTTCTTTCTGTTTTTTCCTACCATGAGTTAGGCAA-TACCCTCTCACCCTTCTTACTACT 323 1 958 ........N..-.................AN......N.............A.G...........A....T.T..N..N......N.... 870 2 35 .............................-...................................-........................ 122 0 198 .............................-...................................-........................ 285 L D H S G P G K I A P S M K R R G V N D H P H R G F E T V T Query_1 324 CGACCATTCAGGTCCCGGAAAAATTGCACCTTCTATGAAACGTAGA-GGAGTTAATGATCATCCCCATCGTGGATTCGAAACGGTAACAC 412 1 869 T..........................-............N.....A...............N.................-......... 782 2 123 ..............................................-........................................... 211 0 286 ............................................N.-........................................... 374 L V Y A G E L E H K D S S G G G G F I S A G D V Q W M T A A Query_1 413 TTGTTTATGCAGGTGAACTAGAACACAAAGATTCATCTGGTGGAGGTGGATTTATAAGTGCTGGTGATGTGCAATGGATGACCGCAGCCT 502 1 781 .......................................................................................... 692 2 212 .......................................................................................... 301 0 375 .......................................................................................... 464 S G V I H R E L F S E E Y S R S G G P F E M I Q L W V N L P Query_1 503 CTGGTGTGATTCATCGGGAATTGTTTAGTGAAGAGTATTCTCGATCTGGTGGTCCTTTTGAAATGATTCAACTTTGGGTCAATTTACCAG 592 1 691 .......................................................................................... 602 2 302 .......................................................................................... 391 0 465 .......................................................................................... 554 A A N K M N S P R Y Q S L K K A E I P M V K L E N E A G F V Query_1 593 CAGCAAATAAAATGAACTCTCCGCGCTATCAAAGTTTAAAGAAAGCTGAAATACCGATGGTGAAACTGGAAAATGAGGCTGGA-TTTGTA 681 1 601 ...................................................................................-...... 513 2 392 ...................................................................................-...... 480 0 555 ..............................N....................................................T...... 644 R I I A G Q F N H V I G P A L T H T P I T V L D V E L H A G Query_1 682 CGTATTATTGCAGGGCAGTTTAACCATGTTATAGGTCCTGCACTTACTCACACTCCTATTACCGTATTAGATGTAGAGCTCCATGCAGGT 771 1 512 .......................................................................................... 423 1 267 ............ 278 2 481 .......................................................................................... 570 2 726 ............ 715 0 645 .................N................................................................ 726 Q Q A A F P A Q S G E T A L V Y L R S G R A Q F Q K D E D V Query_1 772 CAACAAGCCGCCTTCCCTGCTCAATCTGGTGAAACAGCTTTAGTTTATTTACGTTCAGGTCGGGCACAATTTCAAAAAGATGAAGACGTT 861 1 422 .......................................................................................... 333 2 571 .......................................................................................... 660 L E E Q G L A V M S N Q G E H F S I T A L Q S C K L L I L T Query_1 862 TTAGAGGAACAAGGCCTAGCAGTCATGTCAAACCAAGGAGAGCATTTCTCTATTACTGCCCTGCAAAGTTGTAAATTACTTATTTTGACA 951 1 332 .......................................................................................... 243 1 494 ............ 505 2 661 .......................................................................................... 750 2 499 ............ 488 G Q P I T E P I N G H G P F V M N T Y D E I L Q A Y D D I K Query_1 952 GGACAACCCATCACAGAACCGATTAATGGTCATGGTCCATTTGTAATGAATACTTATGATGAGATTTTACAAGCCTATGATGATATTAAA 1041 1 242 .......................................................................................... 153 2 751 .......................................................................................... 840 N G R F A K * H W K W I T D P N S S S V D K L A A A L E H H Query_1 1042 AATGGAAGATTTGCCAAGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccac 1131 1 152 .................................................................N.N...................... 63 2 841 .................. 858 H H H H * D P A A N K A R Query_1 1132 caccaccaccacTGAGATCCGGCTGCTAACAAA-GCCCGAAA 1172 1 62 .................................N...NN... 21 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3201049 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 2,615 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95892.103 (IDP95892.30-549.P(549)..pMCSG68)
QIINYQRAQEYRESLTDGISYVISEGVARQPGKQQKIDWISDASDLLELPIYYTDASKVELSRTEKKRIEAQKSVVRYDASNSIAYVIIGLRDDPQHYLSIKVDKITERQMKALPIFVLDYLMFYPGQEQEYLAKIQKHFSYPINIQNIQDVNLDSEQIGRLRQDQSVMLYKDSATVRGTTISIVSPIPNHPAQVLVLGPVPMFNWMPLQLSAGITLFSLFLLSLGVYGLILPLERKIRQVRYALNRMKSGDLSLRVPIEGSDEMANLASSYNNMSDHIQRLIEAQRELMRAVSHELRTPVARIRFGTEMLAEEDDYNHRMHQVDMIDKDIEALNTLIDEIMTYAKLEQGTPSLDFAEIVLFEVLDQVAVETEALKTQKEIELIPPPLYVKVDAERRYLHRVVQNLVGNAVRYCDNKVRITGGIHNDGMAFVCVEDDGPGIPEQDRKRVFEAFARLDDSRTRASGGYGLGLSIVSRIAYWFGGEIKVDESPSLGGARFIMTWPAHRFKQPPLKTNKKAPA
CAAATTATCAACTACCAACGCGCACAAGAATACCGAGAATCTTTAACTGATGGTATTTCTTATGTCATTAGTGAAGGAGTCGCTCGACAACCTGGGAAGCAGCAAAAAATAGATTGGATTTCTGACGCATCTGATTTGCTCGAACTTCCAATTTATTATACCGATGCAAGTAAGGTTGAGCTGTCTCGGACCGAGAAAAAGCGGATAGAAGCACAAAAATCTGTAGTTCGTTACGATGCAAGCAATAGCATTGCATATGTCATTATTGGCTTACGTGACGACCCACAACATTACTTATCTATAAAAGTCGACAAAATTACTGAACGCCAAATGAAGGCTCTTCCTATTTTTGTGCTCGACTATTTAATGTTTTATCCGGGGCAGGAACAAGAATATCTTGCCAAGATTCAAAAGCATTTCTCATATCCGATCAATATTCAAAATATTCAAGATGTAAATCTGGATTCTGAACAGATTGGACGTTTGCGCCAAGACCAAAGTGTCATGTTGTACAAAGATAGCGCAACAGTGCGCGGTACAACCATTTCGATTGTGTCTCCAATACCGAATCATCCTGCGCAAGTGTTAGTGCTGGGTCCGGTTCCAATGTTTAACTGGATGCCTTTACAGCTTTCGGCGGGTATTACCTTATTTAGCTTATTCTTATTAAGTCTAGGTGTTTACGGTTTGATTTTGCCGTTAGAGCGCAAAATCCGACAGGTGCGTTATGCATTAAACCGTATGAAATCGGGTGATTTGTCACTGCGTGTTCCTATTGAGGGAAGCGATGAAATGGCAAACTTGGCTTCAAGTTATAACAATATGTCTGACCATATTCAGCGTTTGATTGAGGCTCAACGTGAGTTAATGAGAGCAGTATCTCATGAACTCAGAACACCTGTGGCACGTATTCGCTTTGGTACAGAAATGTTAGCCGAAGAAGATGATTACAATCATCGTATGCATCAGGTCGACATGATTGATAAAGATATTGAAGCGCTCAATACCTTAATTGATGAAATCATGACTTATGCAAAACTAGAGCAGGGTACACCTTCATTAGACTTTGCAGAAATTGTACTTTTTGAAGTGTTGGATCAGGTTGCAGTTGAAACTGAGGCTTTAAAAACACAGAAAGAAATTGAACTTATTCCACCGCCTTTATATGTGAAAGTAGATGCAGAGCGTCGTTACCTTCACCGTGTCGTGCAAAATCTAGTAGGTAATGCAGTTCGTTATTGTGATAACAAAGTTCGGATTACGGGCGGTATTCATAATGATGGCATGGCTTTTGTCTGTGTCGAAGACGACGGACCGGGTATTCCTGAGCAAGACCGCAAGCGTGTATTTGAAGCCTTTGCCCGTTTAGATGATAGCCGTACACGTGCATCTGGCGGTTATGGTTTGGGTCTCTCTATCGTAAGTCGTATTGCTTACTGGTTTGGTGGGGAAATTAAGGTAGATGAAAGTCCAAGTTTGGGAGGCGCACGCTTTATTATGACTTGGCCAGCACATCGCTTTAAACAACCGCCATTAAAGACCAATAAAAAAGCACCTGCA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,169 total letters Query= IDP95892.103 Length=1965 Score E Sequences producing significant alignments: (Bits) Value wt 2814 0.0 01C_C1_IDP95892.103_T7short.seq 1819 0.0 07C_C1_IDP95892.103_T7term.seq 1788 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 60 CCCCTCTAGAAATAATTTT-GTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGT 148 0 26 ...T...N....A......T...................................................................... 115 S H P Q F E K G T E N L Y F Q S N A Q I I N Y Q R A Q E Y R Query_1 149 CTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAAATTATCAACTACCAACGCGCACAAGAATACCGAG 238 2 1 ..................................... 37 0 116 .......................................................................................... 205 E S L T D G I S Y V I S E G V A R Q P G K Q Q K I D W I S D Query_1 239 AATCTTTAACTGATGGTATTTCTTATGTCATTAGTGAAGGAGTCGCTCGACAACCTGGGAAGCAGCAAAAAATAGATTGGATTTCTGACG 328 2 38 .......................................................................................... 127 0 206 .......................................................................................... 295 A S D L L E L P I Y Y T D A S K V E L S R T E K K R I E A Q Query_1 329 CATCTGATTTGCTCGAACTTCCAATTTATTATACCGATGCAAGTAAGGTTGAGCTGTCTCGGACCGAGAAAAAGCGGATAGAAGCACAAA 418 2 128 .......................................................................................... 217 2 356 ........ 349 0 296 ............................................................................N............. 385 0 524 ........ 517 K S V V R Y D A S N S I A Y V I I G L R D D P Q H Y L S I K Query_1 419 AATCTGTAGTTCGTTACGATGCAAGCAATAGCATTGCATATGTCATTATTGGCTTACGTGACGACCCACAACATTACTTATCTATAAAAG 508 2 218 .......................................................................................... 307 2 348 ... 346 0 386 .......................................................................................... 475 0 516 ... 514 V D K I T E R Q M K A L P I F V L D Y L M F Y P G Q E Q E Y Query_1 509 TCGACAAAATTACTGAACGCCAAATGAAGGCTCTTCCTATTTTTGTGCTCGACTATTTAATGTTTTATCCGGGGCAGGAACAAGAATATC 598 2 308 .......................................................................................... 397 2 220 ........... 210 0 476 .......................................................................................... 565 0 388 ........... 378 L A K I Q K H F S Y P I N I Q N I Q D V N L D S E Q I G R L Query_1 599 TTGCCAAGATTCAAAAGCATTTCTCATATCCGATCAATATTCAAAATATTCAAGATGTAAATCTGGATTCTGAACAGATTGGACGTTTGC 688 2 398 .......................................................................................... 487 0 566 .......................................................................................... 655 R Q D Q S V M L Y K D S A T V R G T T I S I V S P I P Query_1 689 GCCAAGACCAAAGTGTCATGTTGTACAAA-GATAGCGCAACA----GTGCGCGGTACAA--CCATTTCGATT-GTGTCT-CCAATACCGA 769 2 488 .............................-............----.............--...........-......-.......... 568 0 656 .............................-............----.............--...........-......-.......... 736 1 1214 .....N..........NCNGGT.C......N.NC.NN.N....N...NN.N....T...N...... 1149 N H P A Q V L V L G P V P M F N W M P L Q L S A G I Query_1 770 AT-CATCCTG-CGCAAGT-GTTAGTGCTGGG-TCCGG-TTCCAATG-TTTAACTGG-ATGCC-TTTACAG-CTTTCGG-CGGGTA-TTAC 848 2 569 ..-.......-.......-............-.....-........-.........-.....-.......-.......-......-.... 647 0 737 ..-.......-.......-............-.....-........-.........-.....-.......-.......-......-.... 815 1 1148 ..T.......GN.....NN..NN...N....G.....GNN..N.N.N.........G.....C.......G.......G....N.A.... 1059 T L F S L F L L S L G V Y G L I L P L E R K I R Q V R Query_1 849 CTTA-TTTAG-CTTA-TTCTTATTAAGTCTAGG-TGTTTACGGTTTGATTTTGCCGTTA-GAGCGCAAAA-T-CCG-ACAGG-TGCGTTA 929 2 648 ....-.....-....-.................-.........................-..........-.-...-.....-....... 728 0 816 ....-.....-....-.................-.........................N..........-.C...-.....T....... 899 1 1058 .G..A.....N..N.N.................G.....N...G...N...........-..........A.-..NN...N.-....... 972 Y A L N R M K S G D L S L R V P I E G S D E M A N L A S S Query_1 930 TGCATTAAACCGTATGAAATCGGGTGATTTGTCACTGCGTGTTCCTATTGAGGGAAGCGATGAAATGGC--AAACTTGGCTTCAAGTTA- 1016 2 729 .....................................................................--..................- 815 0 900 ..................N..................................................--..N....NN.........- 986 1 971 ........-...................................................N.......GCA..................A 883 Y N N M S D H I Q R L I E A Q R E L M R A V S H E L R T Query_1 1017 TAACAATATGTCTGACCATATT-CAGCGTTTGATTGAGGCTCAACGT-GAGTTAATGAGAG-CAGTATCTCATGAA-CTCAG-AACACC- 1100 2 816 ......................-........................-.............-..............-.....-......- 899 0 987 .......N..............C...................N....G......T......N.........NG...C...N.N......C 1076 1 882 ......................-........................-.............-..............-.....-......- 799 P V A R I R F G T E M L A E E D D Y N H R M H Q V D M Query_1 1101 -TGTGGC-ACGTATT-CGCTTTGGTACAGAAA--TGTTAGCCGAAGAAGATGATTACAAT-CA-TCGTATG-CATCAGGTC-GACATGAT 1181 2 900 -......-.......-................--..........................-..-.......-.........-........ 980 0 1077 TG.....NN......N.....G...N.-....NNNN..C..............A...C..N..T....N..C.N.N.....N...NNN.A 1165 1 798 -......-.N.....-................--..........................-..-.......-.........-........ 718 I D K D I E A L N T L I D E I M T Y A K L E Q G T P S L Query_1 1182 TG--ATAAAGATATT-GAAGCGCT-CAATACC-TTAA-TTGATGAAATCATGACTTATGCAAAACTAGAGCAGGGTACACCTTCATTAGA 1265 2 981 ..--...........-........-.......-....-.................................................... 1064 0 1166 ..GAN...G......G..NN...NC..T....T....-............. 1215 1 717 ..--...........-........-.......-....A.................................................... 633 D F A E I V L F E V L D Q V A V E T E A L K T Q K E I E L I Query_1 1266 CTTTGCAGAAATTGTACTTTTTGAAGTGTTGGATCAGGTTGCAGTTGAAACTGAGGCTTTAAAAACACAGAAAGAAATTGAACTTATTCC 1355 2 1065 .......................................................................................... 1154 1 632 .......................................................................................... 543 P P P L Y V K V D A E R R Y L H R V V Q N L V G N A V R Y C Query_1 1356 ACCGCCTTTATATGTGAAAGTAGATGCAGAGCGTCGTTACCTTCACCGTGTCGTGCAAAATCTAGTAGGTAATGCAGTTCGTTATTGTGA 1445 2 1155 .......................................................................................... 1244 1 542 .......................................................................................... 453 D N K V R I T G G I H N D G M A F V C V E D D G P G I P E Q Query_1 1446 TAACAAAGTTCGGATTACGGGCGGTATTCATAATGATGGCATGGCTTTTGTCTGTGTCGAAGACGACGGACCGGGTATTCCTGAGCAAGA 1535 2 1245 .......................................................................................... 1334 1 452 .......................................................................................... 363 D R K R V F E A F A R L D D S R T R A S G G Y G L G L S I V Query_1 1536 CCGCAAGCGTGTATTTGAAGCCTTTGCCCGTTTAGATGATAGCCGTACACGTGCATCTGGCGGTTATGGTTTGGGTCTCTCTATCGTAAG 1625 2 1335 .......................................................................................... 1424 1 362 .......................................................................................... 273 S R I A Y W F G G E I K V D E S P S L G G A R F I M T W P A Query_1 1626 TCGTATTGCTTACTGGTTTGGTGGGGAAATTAAGGTAGATGAAAGTCCAAGTTTGGGAGGCGCACGCTTTATTATGACTTGGCCAGCACA 1715 2 1425 .......................................................................................... 1514 1 272 .......................................................................................... 183 H R F K Q P P L K T N K K A P A * H W K W I T D P N S S S V Query_1 1716 TCGCTTTAAACAACCGCCATTAAAGACCAATAAAAAAGCACCTGCATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGA 1805 2 1515 .............................................. 1560 1 182 .......................................................................................... 93 D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 1806 CAAGCTTGCGGCCGCACTCGAGcaccaccaccaccacc-acTGAGATCCGGCTGCT-AACAAAG-CCCGAAAGGAA 1878 1 92 ......................................A.................A.......C.......A... 17 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 8021864 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 4,169 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95892.105 (IDP95892.260-549.P(549)..pMCSG68)
ILPLERKIRQVRYALNRMKSGDLSLRVPIEGSDEMANLASSYNNMSDHIQRLIEAQRELMRAVSHELRTPVARIRFGTEMLAEEDDYNHRMHQVDMIDKDIEALNTLIDEIMTYAKLEQGTPSLDFAEIVLFEVLDQVAVETEALKTQKEIELIPPPLYVKVDAERRYLHRVVQNLVGNAVRYCDNKVRITGGIHNDGMAFVCVEDDGPGIPEQDRKRVFEAFARLDDSRTRASGGYGLGLSIVSRIAYWFGGEIKVDESPSLGGARFIMTWPAHRFKQPPLKTNKKAPA
ATTTTGCCGTTAGAGCGCAAAATCCGACAGGTGCGTTATGCATTAAACCGTATGAAATCGGGTGATTTGTCACTGCGTGTTCCTATTGAGGGAAGCGATGAAATGGCAAACTTGGCTTCAAGTTATAACAATATGTCTGACCATATTCAGCGTTTGATTGAGGCTCAACGTGAGTTAATGAGAGCAGTATCTCATGAACTCAGAACACCTGTGGCACGTATTCGCTTTGGTACAGAAATGTTAGCCGAAGAAGATGATTACAATCATCGTATGCATCAGGTCGACATGATTGATAAAGATATTGAAGCGCTCAATACCTTAATTGATGAAATCATGACTTATGCAAAACTAGAGCAGGGTACACCTTCATTAGACTTTGCAGAAATTGTACTTTTTGAAGTGTTGGATCAGGTTGCAGTTGAAACTGAGGCTTTAAAAACACAGAAAGAAATTGAACTTATTCCACCGCCTTTATATGTGAAAGTAGATGCAGAGCGTCGTTACCTTCACCGTGTCGTGCAAAATCTAGTAGGTAATGCAGTTCGTTATTGTGATAACAAAGTTCGGATTACGGGCGGTATTCATAATGATGGCATGGCTTTTGTCTGTGTCGAAGACGACGGACCGGGTATTCCTGAGCAAGACCGCAAGCGTGTATTTGAAGCCTTTGCCCGTTTAGATGATAGCCGTACACGTGCATCTGGCGGTTATGGTTTGGGTCTCTCTATCGTAAGTCGTATTGCTTACTGGTTTGGTGGGGAAATTAAGGTAGATGAAAGTCCAAGTTTGGGAGGCGCACGCTTTATTATGACTTGGCCAGCACATCGCTTTAAACAACCGCCATTAAAGACCAATAAAAAAGCACCTGCA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,501 total letters Query= IDP95892.105 Length=1275 Score E Sequences producing significant alignments: (Bits) Value 01D_D1_IDP95892.105_T7short.seq 1651 0.0 wt 1570 0.0 07D_D1_IDP95892.105_T7term.seq 838 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 60 CCCCTCT-AGAAATAATTTT-GTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGG 147 0 23 .......TN...........T..................................................................... 112 S H P Q F E K G T E N L Y F Q S N A I L P L E R K I R Q V R Query_1 148 TCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATTTTGCCGTTAGAGCGCAAAATCCGACAGGTGCGT 237 0 113 .......................................................................................... 202 2 1 .................................... 36 Y A L N R M K S G D L S L R V P I E G S D E M A N L A S S Y Query_1 238 TATGCATTAAACCGTATGAAATCGGGTGATTTGTCACTGCGTGTTCCTATTGAGGGAAGCGATGAAATGGCAAACTTGGCTTCAAGTTAT 327 0 203 .......................................................................................... 292 2 37 .......................................................................................... 126 N N M S D H I Q R L I E A Q R E L M R A V S H E L R T P V A Query_1 328 AACAATATGTCTGACCATATTCAGCGTTTGATTGAGGCTCAACGTGAGTTAATGAGAGCAGTATCTCATGAACTCAGAACACCTGTGGCA 417 0 293 .......................................................................................... 382 2 127 .......................................................................................... 216 R I R F G T E M L A E E D D Y N H R M H Q V D M I D K D I E Query_1 418 CGTATTCGCTTTGGTACAGAAATGTTAGCCGAAGAAGATGATTACAATCATCGTATGCATCAGGTCGACATGATTGATAAAGATATTGAA 507 0 383 .......................................................................................... 472 2 217 .......................................................................................... 306 A L N T L I D E I M T Y A K L E Q G T P S L D F A E I V L F Query_1 508 GCGCTCAATACCTTAATTGATGAAATCATGACTTATGCAAAACTAGAGCAGGGTACACCTTCATTAGACTTTGCAGAAATTGTACTTTTT 597 0 473 .......................................................................................... 562 2 307 .......................................................................................... 396 E V L D Q V A V E T E A L K T Q K E I E L I P P P L Y V K Query_1 598 GAAGTGTTGGATCAGGTTGCAGTTGAAACTGAGGCTTTAAAAACACAGAAAGAAA-TTGAACTTATTCCACCGCCTTTATATGTGAAAGT 686 0 563 ..............................................N........T.................................. 652 2 397 .......................................................-.................................. 485 1 517 ..................... 497 V D A E R R Y L H R V V Q N L V G N A V R Y C D N K V R I T Query_1 687 AGATGCAGAGCGTCGTTACCTTCACCGTGTCGTGCAAAATCTAGTAGGTAATGCAGTTCGTTATTGTGATAAC-AAAGTTCGGATTACGG 775 0 653 ..........................CG.............................................A................ 742 2 486 .........................................................................-................ 574 1 496 ..............................N....................N..............N......-................ 408 G G I H N D G M A F V C V E D D G P G I P E Q D R K R V F Query_1 776 GCGGTA-TTCATAATGAT-GGCATGGC-TTTTGTCTGTGTCGAAGACGACGGACCGGGTATTCCTGAGCAAGACCGCAAGCGTGTATTTG 862 0 743 ......T...........G........T...................N...................-.........N.......TA... 831 2 575 ......-...........-........-.............................................................. 661 1 407 ......-...........-........-......N........................................A.............. 321 E A F A R L D D S R T R A S G G Y G L G L S I V S R I A Y Query_1 863 AAGCC-TTTGCCCGTTTAGATGATAGCCGTACACGTG-CATCTGGC-GGTTATGGTTTGGGTCTCTCTATCGTAAGTCGTATTGCTTACT 949 0 832 .....T...............................N........G.......T..G.........C....-..............N.. 920 2 662 .....-...............................-........-........................................... 748 1 320 .....-....N..........................-........-..................................N......N. 234 W F G G E I K V D E S P S L G G A R F I M T W P A H R F K Query_1 950 GGTTTGGTGGGGAAATTAAGGTAGATGAAAGTCCAAGTTTGGG-AGGCGCACGCTTTATTATGACTTGGCCAGCA-CATCGCTTTAAACA 1037 0 921 ..............................N.-..........A........N..................C.G.N.TN......-N... 1008 2 749 ...........................................-...............................-.............. 836 1 233 ...........................................-....................N..........-.............. 146 Q P P L K T N K K A P A * H W K W I T D P N S S S V D K Query_1 1038 ACCGCCATTAAAGACCAAT-AAAAAAGCA-CCTGCAT-AACATTGGAAGTGGATA-ACGG-ATCCGAA--TTCGAGCTCCGTCG-ACAAG 1119 0 1009 ...TG.C....G.......N.........C.......A.....G...........T....A.......AT.N.ANN....N...A.N... 1098 2 837 ...................-.........-...... 870 1 145 ...................-.........-.......-.................-....-...N...--..............-....N 64 L A A A L E H H H H H H * D P Query_1 1120 C-TTGCGGCCGCACTCGAGcaccaccaccaccaccacT-GAGATCCGG 1165 0 1099 N-......G..G..... 1114 1 63 NA.N...N..............................G......... 16 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3094560 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 2,501 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95895.103 (IDP95895.25-394.P(394)..pMCSG68)
APVVKEKLSPLHPDSPESEESYAEVPIESIQQFVQIYGIVRDNYVDEKSDDALFLQAIKGLVSGLDRYSRYLSAEEYRQLIQYTEGDLASVDFVLSPESHVHKWMIRDLKTGSDSYKLGLRNGQTILKIDNQELKNLTHDQVLGLLYGSIGSTLQVQTEESNSPISLVRNKKIETDIEPVMLHNQVLVLKIRVFQQDTANEIKRLIEENSSSRLKAVLIDLRNNPGGLLSAAVESADLFLNHGIIVSTKSRSEGNQQFQALPGNDFQNIKVGILINHRSASAAEVFTAAMKEHQRAWVMGEKSYGKGVVQKLFPLPSGAALQMTVSHYYTPNGNMIEGQGIQPNQTYPLPPEMKEEVYLDRVADLLLKRK
GCTCCTGTTGTAAAAGAAAAATTATCGCCTCTGCATCCTGATTCCCCGGAATCAGAAGAAAGTTATGCAGAAGTGCCTATTGAGTCTATTCAACAGTTTGTACAAATATATGGGATTGTCCGTGATAATTATGTCGATGAAAAATCGGACGATGCTTTATTTTTACAGGCAATTAAAGGTTTAGTCAGTGGTCTAGACCGTTACTCCCGCTATTTGTCAGCTGAAGAATACCGTCAACTCATTCAATATACTGAAGGGGATCTTGCTTCAGTTGATTTTGTACTAAGCCCCGAATCACATGTTCATAAATGGATGATTCGTGACCTTAAGACGGGTTCTGACTCATATAAACTAGGCTTAAGAAATGGCCAAACTATTTTAAAGATTGATAATCAGGAACTTAAAAATCTGACACATGATCAGGTGCTAGGTTTGCTTTATGGATCGATAGGCTCGACGCTTCAAGTACAAACAGAAGAGTCAAATAGTCCAATTAGTTTAGTCCGTAATAAAAAGATTGAAACCGATATTGAGCCTGTGATGTTGCACAATCAGGTGTTGGTATTAAAAATTAGAGTATTTCAACAAGATACCGCCAATGAGATTAAGCGTTTAATTGAAGAAAATAGTTCTTCGCGTTTAAAAGCGGTGTTAATTGATTTAAGAAATAACCCAGGCGGCTTGTTATCTGCTGCTGTTGAGTCAGCCGATTTATTTTTAAATCATGGCATTATTGTCTCTACAAAAAGTCGTTCAGAAGGCAATCAGCAATTTCAGGCTTTGCCAGGTAACGATTTTCAAAATATAAAAGTTGGTATTTTAATTAATCACCGATCTGCCTCGGCAGCAGAAGTTTTTACTGCTGCAATGAAAGAGCATCAACGTGCTTGGGTCATGGGTGAAAAAAGTTATGGAAAGGGTGTTGTGCAAAAGCTTTTCCCTCTACCAAGTGGGGCAGCGCTACAAATGACAGTCTCACATTACTACACACCAAATGGTAATATGATTGAGGGGCAAGGTATTCAGCCTAACCAGACTTATCCCTTACCCCCAGAAATGAAAGAAGAAGTTTATCTAGATCGTGTTGCCGATCTCCTTCTAAAAAGAAAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,313 total letters Query= IDP95895.103 Length=1515 Score E Sequences producing significant alignments: (Bits) Value wt 2003 0.0 01E_E1_IDP95895.103_T7short.seq 1171 0.0 07E_E1_IDP95895.103_T7term.seq 792 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 58 TTCCCCTCTAGAAATAATTTT--GTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGT 145 0 18 .....T...............TT..............................................N.................... 107 W S H P Q F E K G T E N L Y F Q S N A A P V V K E K L S P L Query_1 146 GGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGCTCCTGTTGTAAAAGAAAAATTATCGCCTCTGC 235 2 1 .................................. 34 0 108 .......................................................................................... 197 H P D S P E S E E S Y A E V P I E S I Q Q F V Q I Y G I V R Query_1 236 ATCCTGATTCCCCGGAATCAGAAGAAAGTTATGCAGAAGTGCCTATTGAGTCTATTCAACAGTTTGTACAAATATATGGGATTGTCCGTG 325 2 35 .......................................................................................... 124 0 198 ...................................N......................................CG.............. 287 D N Y V D E K S D D A L F L Q A I K G L V S G L D R Y S R Y Query_1 326 ATAATTATGTCGATGAAAAATCGGACGATGCTTTATTTTTACAGGCAATTAAAGGTTTAGTCAGTGGTCTAGACCGTTACTCCCGCTATT 415 2 125 .......................................................................................... 214 0 288 .......................................................................................... 377 L S A E E Y R Q L I Q Y T E G D L A S V D F V L S P E S H V Query_1 416 TGTCAGCTGAAGAATACCGTCAACTCATTCAATATACTGAAGGGGATCTTGCTTCAGTTGATTTTGTACTAAGCCCCGAATCACATGTTC 505 2 215 .......................................................................................... 304 0 378 .....N.........................N.........N..N...........................N................. 467 H K W M I R D L K T G S D S Y K L G L R N G Q T I L K I D N Query_1 506 ATAAATGGATGATTCGTGACCTTAAGACGGGTTCTGACTCATATAAACTAGGCTTAAGAAATGGCCAAACTATTTTAAAGATTGATAATC 595 2 305 .......................................................................................... 394 0 468 .........................N..............N..........................N...................... 557 Q E L K N L T H D Q V L G L L Y G S I G S T L Q V Q T E E S Query_1 596 AGGAACTTAAAAATCTGACACATGATCAGGTGCTAGGTTTGCTTTATGGATCGATAGGCTCGACGCTTCAAGTACAAACAGAAGAGTCAA 685 2 395 .......................................................................................... 484 0 558 ...........................................................................N...N.......... 647 N S P I S L V R N K K I E T D I E P V M L H N Q V L V L K Query_1 686 ATAGTCCAATTAGTTTAGTCCGTAATAAAAAGATTGAAACCGATATTG-AGCCTGTGATGTTGCACA-ATCAGGTGTTGGTATTAAAAAT 773 2 485 ................................................-..................-...................... 572 0 648 ...N...CCN.......................N..............A...............T..T............ 727 I R V F Q Q D T A N E I K R L I E E N S S S R L K A V L I D Query_1 774 TAGAGTATTTCAACAAGATACCGCCAATGAGATTAAGCGTTTAATTGAAGAAAATAGTTCTTCGCGTTTAAAAGCGGTGTTAATTGATTT 863 2 573 .......................................................................................... 662 L R N N P G G L L S A A V E S A D L F L N H G I I V S T K S Query_1 864 AAGAAATAACCCAGGCGGCTTGTTATCTGCTGCTGTTGAGTCAGCCGATTTATTTTTAAATCATGGCATTATTGTCTCTACAAAAAGTCG 953 2 663 .......................................................................................... 752 1 476 .......N. 468 R S E G N Q Q F Q A L P G N D F Q N I K V G I L I N H R S A Query_1 954 TTCAGAAGGCAATCAGCAATTTCAGGCTTTGCCAGGTAACGATTTTCAAAATATAAAAGTTGGTATTTTAATTAATCACCGATCTGCCTC 1043 2 753 .......................................................................................... 842 1 467 ..............................N........................................................... 378 S A A E V F T A A M K E H Q R A W V M G E K S Y G K G V V Q Query_1 1044 GGCAGCAGAAGTTTTTACTGCTGCAATGAAAGAGCATCAACGTGCTTGGGTCATGGGTGAAAAAAGTTATGGAAAGGGTGTTGTGCAAAA 1133 2 843 .......................................................................................... 932 1 377 .N............................................N.....................................N..... 288 K L F P L P S G A A L Q M T V S H Y Y T P N G N M I E G Q G Query_1 1134 GCTTTTCCCTCTACCAAGTGGGGCAGCGCTACAAATGACAGTCTCACATTACTACACACCAAATGGTAATATGATTGAGGGGCAAGGTAT 1223 2 933 .......................................................................................... 1022 1 287 .............N.............N.............................................................. 198 I Q P N Q T Y P L P P E M K E E V Y L D R V A D L L L K R K Query_1 1224 TCAGCCTAACCAGACTTATCCCTTACCCCCAGAAATGAAAGAAGAAGTTTATCTAGATCGTGTTGCCGATCTCCTTCTAAAAAGAAAGTA 1313 2 1023 ........................................................................................ 1110 1 197 ....................................................N.......N...N...........N............. 108 * H W K W I T D P N S S S V D K L A A A L E H H H H H H * D Query_1 1314 ACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCC 1403 1 107 .......................................................................................... 18 P Query_1 1404 GG 1405 1 17 .. 16 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3395235 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 2,313 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96090.102 (IDP96090.1-504.F..pMCSG68)
MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGAGKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAENIYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALARNAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKDGRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPISLAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMMLCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPGAEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGVAVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA
ATGCAACAGTCTACCCCGTATCTCTCATTTCGCGGCATCGGTAAAACGTTTCCCGGCGTTAAGGCGCTGACGGATATTAGTTTTGACTGCTATGCCGGTCAGGTTCATGCGTTGATGGGTGAAAATGGCGCAGGAAAATCAACTCTCTTAAAAATCCTCAGCGGCAACTATGCGCCAACCACGGGTTCTGTAGTGATTAATGGGCAGGAAATGTCCTTTTCCGACACGACCGCAGCACTTAACGCGGGCGTGGCGATTATTTACCAGGAACTGCATCTCGTGCCGGAAATGACCGTCGCGGAAAACATCTATCTCGGCCAGCTGCCGCATAAAGGCGGCATTGTGAATCGCTCATTGCTGAATTATGAGGCGGGTTTACAACTTAAACATCTTGGTATGGATATTGACCCGGACACGCCGCTGAAATATCTCTCCATTGGTCAGTGGCAGATGGTTGAAATCGCCAAAGCGCTGGCGCGTAACGCCAAAATTATCGCCTTTGATGAGCCAACCAGCTCCCTCTCTGCCCGTGAAATCGACAATCTTTTCCGCGTTATTCGTGAACTGCGAAAAGAGGGGCGGGTAATCTTATACGTTTCTCACCGTATGGAAGAAATATTTGCCCTCAGCGATGCCATTACTGTCTTTAAAGATGGACGTTATGTCAAAACCTTTACCGATATGCAGCAGGTTGACCACGACGCGCTGGTGCAGGCGATGGTCGGGCGCGACATTGGCGATATCTACGGCTGGCAACCGCGTAGTTATGGCGAGGAGCGCCTACGTCTTGATGCTGTGAAAGCACCAGGCGTGCGTACGCCAATAAGTCTGGCGGTTCGCAGTGGTGAAATTGTTGGGCTGTTTGGTCTGGTAGGGGCGGGGCGTAGCGAATTAATGAAAGGCATGTTTGGCGGGACGCAAATCACCGCCGGTCAGGTTTATATCGACCAACAGCCGATCGATATTCGTAAACCGAGCCACGCCATTGCCGCAGGCATGATGCTCTGCCCGGAAGATCGCAAAGCGGAAGGCATTATTCCCGTGCACTCCGTTCGCGACAATATCAACATCAGTGCCAGACGTAAACATGTGCTCGGCGGTTGTGTAATCAACAACGGTTGGGAAGAAAACAATGCCGATCACCACATTCGTTCGCTCAACATCAAAACGCCGGGCGCGGAGCAACTGATCATGAATCTCTCAGGCGGAAATCAGCAAAAAGCCATTCTGGGCCGCTGGTTATCGGAAGAGATGAAGGTCATTTTGCTGGATGAACCTACGCGCGGCATTGATGTTGGCGCTAAGCACGAAATATATAACGTAATTTATGCGCTGGCGGCGCAGGGCGTGGCGGTGCTGTTTGCCTCCAGCGACTTACCTGAAGTCCTCGGCGTTGCCGACCGGATTGTGGTGATGCGGGAAGGTGAAATCGCCGGTGAATTGTTACACGAGCAGGCAGATGAGCGTCAGGCACTGAGCCTTGCGATGCCTAAAGTCAGCCAGGCTGTTGCC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,565 total letters Query= IDP96090.102 Length=1917 Score E Sequences producing significant alignments: (Bits) Value wt 2727 0.0 02G_G2_IDP96090.102_T7short.seq 1649 0.0 08G_G2_IDP96090.102_T7term.seq 1544 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 60 CCCCTC-TAGAAATAATTTTGTTT-AACTTT-AAGAA-GGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGT 145 0 23 ......N.....-...........T......T.....N.................................................... 111 W S H P Q F E K G T E N L Y F Q S N A M Q Q S T P Y L S F R Query_1 146 GGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGCAACAGTCTACCCCGTATCTCTCATTTCGCG 235 2 1 .................................. 34 0 112 .......................................................................................... 201 G I G K T F P G V K A L T D I S F D C Y A G Q V H A L M G E Query_1 236 GCATCGGTAAAACGTTTCCCGGCGTTAAGGCGCTGACGGATATTAGTTTTGACTGCTATGCCGGTCAGGTTCATGCGTTGATGGGTGAAA 325 2 35 .......................................................................................... 124 2 928 ............T.. 942 0 202 .......................................................................................... 291 1 693 ............W.. 679 N G A G K S T L L K I L S G N Y A P T T G S V V I N G Q E M Query_1 326 ATGGCGCAGGAAAATCAACTCTCTTAAAAATCCTCAGCGGCAACTATGCGCCAACCACGGGTTCTGTAGTGATTAATGGGCAGGAAATGT 415 2 125 .......................................................................................... 214 0 292 .......................................................................................... 381 S F S D T T A A L N A G V A I I Y Q E L H L V P E M T V A E Query_1 416 CCTTTTCCGACACGACCGCAGCACTTAACGCGGGCGTGGCGATTATTTACCAGGAACTGCATCTCGTGCCGGAAATGACCGTCGCGGAAA 505 2 215 .......................................................................................... 304 0 382 ...........N.............................................................................. 471 N I Y L G Q L P H K G G I V N R S L L N Y E A G L Q L K H L Query_1 506 ACATCTATCTCGGCCAGCTGCCGCATAAAGGCGGCATTGTGAATCGCTCATTGCTGAATTATGAGGCGGGTTTACAACTTAAACATCTTG 595 2 305 .......................................................................................... 394 0 472 .......................................................................................... 561 G M D I D P D T P L K Y L S I G Q W Q M V E I A K A L A R N Query_1 596 GTATGGATATTGACCCGGACACGCCGCTGAAATATCTCTCCA-TTGGTCAGTGGCAGATGGTTGAAATCGCCAAAGCGCTGGCGCGTAAC 684 2 395 ..........................................-............................................... 483 0 562 ..........................................T............................................... 651 A K I I A F D E P T S S L S A R E I D N L F R V I R E L R Query_1 685 GCCAAAA-TTATCGCCTTTGATGAGCCAACCAGCTCCCTCTCTGCCCGTGAAATCGACAATC-TTTTCCGCGTTATTCGTGAACTGCGAA 772 2 484 .......-......................................................-........................... 571 0 652 .......T..........................................-...........T........................... 740 K E G R V I L Y V S H R M E E I F A L S D A I T V F K D G R Query_1 773 AAGAGGGGCGGGTAATCTTATACGTTTCTCACCGTATGGAAGAAATATTTGCCCTCAGCGATGCCATTACTGTCTTTAAAGATGGACGTT 862 2 572 .......................................................................................... 661 0 741 N.........................................................................-.............-. 828 Y V K T F T D M Q Q V D H D A L V Q A M V G R D I G D I Y Query_1 863 ATGTCAAAACCTTTACCGATATGCAGCAGGTTGACCACGACGCGCTGGTGCAGGC--GATGGTCGGGCGCGACATTGGCGATATCTACGG 950 2 662 .......................................................--................................. 749 0 829 ........C.T..............N..-...................N...N..--.........................N....... 915 1 918 .........GCG................................. 874 G W Q P R S Y G E E R L R L D A V K A P G V R T P I S L A V Query_1 951 CTGGCAACCGCGTAGTTATGGCGAGGAGCGCCTACGTCTTGATGCTGTGAAAGCACCAGGCGTGCGTACGCC-AATAAGTCTGGCGGTTC 1039 2 750 ........................................................................-................. 838 0 916 ....N..T..Y...N.........................................T.......N.-N....A................. 1004 1 873 ..........N..........N............................................N.....-................. 785 R S G E I V G L F G L V G A G R S E L M K G M F G G T Query_1 1040 GCAGTGGT-GAAATTGTT-GGG-CTGTTTGGTCTGGT-AGGGGC-GGGG-CGTAGCGAATTAATGAAAG--GCATG-TTTGGCG-GGACG 1119 2 839 ........-.........-...-..............-......-....-...................--.....-.......-..... 918 0 1005 ........N.......N.A..N-..NG........C.N......T..T.-N..TT.NT.......NN..CNNNN..T...T...T.T... 1092 1 784 ........-....A..N.-...G.........N...N-......-....G...................--.....-.......-..... 703 Q I T A G Q V Y I D Q Q P I D I R K P S H A I A A G M M L Query_1 1120 -CAAATCACCGCCGGTC--AGGTTTATATCGACCAACAGCCGATCGATATTCGTAAACCGAGCCACGCCATTGCCGCAGGCATGATGCTC 1206 2 919 -................--....................................................................... 1005 2 94 .......--.....C.. 108 0 1093 N...NN.NN........NN...... 1117 0 261 .......--.....C.. 275 1 702 -................--.....W................................................................. 616 C P E D R K A E G I I P V H S V R D N I N I S A R R K H V L Query_1 1207 TGCCCGGAAGATCGCAAAGCGGAAGGCATTATTCCCGTGCACTCCGTTCGCGACAATATCAACATCAGTGCCAGACGTAAACATGTGCTC 1296 2 1006 .......................................................................................... 1095 1 615 .......................................................................................... 526 G G C V I N N G W E E N N A D H H I R S L N I K T P G A E Q Query_1 1297 GGCGGTTGTGTAATCAACAACGGTTGGGAAGAAAACAATGCCGATCACCACATTCGTTCGCTCAACATCAAAACGCCGGGCGCGGAGCAA 1386 2 1096 .......................................................................................... 1185 1 525 .......................................................................................... 436 L I M N L S G G N Q Q K A I L G R W L S E E M K V I L L D E Query_1 1387 CTGATCATGAATCTCTCAGGCGGAAATCAGCAAAAAGCCATTCTGGGCCGCTGGTTATCGGAAGAGATGAAGGTCATTTTGCTGGATGAA 1476 2 1186 .......................................................................................... 1275 1 435 .......................................................................................... 346 P T R G I D V G A K H E I Y N V I Y A L A A Q G V A V L F A Query_1 1477 CCTACGCGCGGCATTGATGTTGGCGCTAAGCACGAAATATATAACGTAATTTATGCGCTGGCGGCGCAGGGCGTGGCGGTGCTGTTTGCC 1566 2 1276 .......................................................................................... 1365 1 345 .......................................................................................... 256 S S D L P E V L G V A D R I V V M R E G E I A G E L L H E Q Query_1 1567 TCCAGCGACTTACCTGAAGTCCTCGGCGTTGCCGACCGGATTGTGGTGATGCGGGAAGGTGAAATCGCCGGTGAATTGTTACACGAGCAG 1656 2 1366 .......................................................................................... 1455 1 255 .......................................................................................... 166 A D E R Q A L S L A M P K V S Q A V A * H W K W I T D P N S Query_1 1657 GCAGATGAGCGTCAGGCACTGAGCCTTGCGATGCCTAAAGTCAGCCAGGCTGTTGCCTAACATTGGAAGTGGATAACGGATCCGAATTCG 1746 2 1456 ......................................................... 1512 1 165 .......................................................................................... 76 S S V D K L A A A L E H H H H H H * D P A Query_1 1747 AGCTCCGTCGAC-AAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCC-GGCTGC 1811 1 75 G...........A.....................---.......................G...... 12 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 6685817 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 3,565 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96096.102 (IDP96096.1-1411.F..pMCSG68)
MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVTSGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADIRLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGRTQTFHREAAGEFSGEITGVTDGAWRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAVVYDRSGKQVRSFTYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLDRREVLHTQGEAGLKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHNQLTSATGPDGLELRREYDELGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQLIAVKDTQGHETRYEYNIAGDLTAVIAPDGSRNGTQYDAWGKAVRTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRLTHRTVKGETAERWQYDERGWLTDISHISEGHRVAVHYRYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGYLAGMKLGDTPLVEYTRDRLHRETLRSFGRYELTTAYTPAGQLQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLTGVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDRHGRLTEKTDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYEEPLVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRSRIQTIYQPGSFTPLIRVETATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLPLALISTEGATAWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYGYQLNPISDIDPLGLSMWEDAKSGACTNGLCGTLSAMIGPDKFDSIDSTAYDALNKINSQSICEDKEFAGLICKDNSGRYFSTAPNRGERKGSYPFNSPCPNGTEKVSAYHTHGADSHGEYWDEIFSGKDEKIVKSKDNNIKSFYLGTPSGNFKAIDNHGKEITNRKGLPNVCRVHGNM
ATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATAACACACTGATACTCAGTGATAACGGCGGCAGAAGCCTGTATTTTGAGCACCTGTTTCCCGGTGAGGACGGTTACAGCCGCAGCGAGTCACTGTGGCTGGTGCGCGGCGGCGTGGCGAAACTGGATGAAGGTCACCGGCTGGCCGCACTCTGGCAGGCGCTGCCGGAAGAACTCCGCTTAAGTCCGCATCGTTATCTGGCGACAAACAGTCCGCAGGGGCCGTGGTGGCTGCTCGGTTGGTGTGAGCGGGTGCCGGAAGCGGATGAGGTGCTGCCTGCGCCGCTGCCGCCGTACCGGGTACTGACCGGGCTGGTGGACCGCTTCGGGCGCACACAGACGTTCCACCGCGAAGCCGCCGGTGAATTCAGCGGCGAAATCACCGGCGTGACGGATGGTGCCTGGCGTCACTTCCGGCTGGTACTGACCACGCAGGCGCAGCGGGCAGAAGAAGCCCGGCAGCAGGCCATTTCCGGCGGGACGGAACCGTCCGCTTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCACGCGGCGAACTGGCGGTGGTGTATGACCGTAGTGGCAAACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGGCCGGAAATCCGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACGCAGAGTCAGTTTGACGCCGTGGGCAGGCTCAGGGCACAGACGGATGCCGCAGGCAGGACAACAGAGTACAGCCCGGATGTGGTGACGGGCCTCATCACGCGCATAACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAACCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAATTGCGCCGGGAATATGATGAATTGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACAGTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACCGACTGTTCCGGTTATGTAACCCGTTATGACCATGACCGCTTCGGGCAGATGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTACCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTGACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCACCTTCCGTTACGATGTACTTGACCGGCTGATACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTATCCGCAGCGAGGATGAGGGTCTGGTCACCCACTGGCACTATGACGAAGCAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTTATACCCCTGCCGGGCAGTTACAGAGCCAGCACCTGAACAGCCTGCTGTCTGACCGCGATTACACCTGGAACGACAACGGCGAACTCATCCGCATCAGCAGCCCGCGCCAGACCCGGAGTTACAGCTACAGCACCACCGGCAGGCTGACCGGCGTTCACACCACCGCAGCGAATCTGGATATCCGCATCCCGTATGCCACAGACCCGGCAGGTAACCGCCTGCCCGACCCGGAGCTGCACCCGGACAGCACCCTCAGCATGTGGCCGGATAACCGTATCGCCCGTGACGCGCACTATCTTTACCGGTATGACCGTCACGGCAGGCTGACAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGGACTCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGAAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGGCGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATGGATATCAATTGAATCCGATATCAGACATCGACCCCCTGGGTTTATCTATGTGGGAGGATGCAAAATCGGGGGCATGTACTAATGGTCTTTGCGGCACACTATCCGCTATGATAGGTCCAGATAAATTTGATTCTATAGATAGCACCGCATATGACGCCTTAAATAAAATAAATAGCCAATCTATTTGCGAAGATAAAGAGTTCGCTGGTTTAATATGTAAGGATAATAGTGGCAGATATTTCTCAACAGCACCTAACCGAGGAGAAAGAAAAGGATCATATCCATTCAATAGCCCTTGCCCTAATGGTACTGAGAAAGTATCAGCTTATCATACTCATGGTGCAGATAGTCATGGAGAATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 6,008 total letters Query= IDP96096.102 Length=4638 Score E Sequences producing significant alignments: (Bits) Value wt 7634 0.0 02H_H2_IDP96096.102_T7short.seq 1483 0.0 08H_H2_IDP96096.102_T7term.seq 1411 0.0 R N N F V * L * E G D I H M H H H H H H S S G V D L W S Query_1 65 CTAGAAATAATTTT-GTTTAAC--TTTAAGAA-GGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCT 150 0 28 ..............T.......NT........A......................................................... 117 H P Q F E K G T E N L Y F Q S N A M S G K P A A R Q G D M T Query_1 151 CATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACG 240 2 1 ....................................... 39 0 118 .......................................................................................... 207 Q Y G G S I V Q G S A G V R I G A P T G V A C S V C P G G V Query_1 241 CAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTG 330 2 40 .......................................................................................... 129 0 208 .......................................................................................... 297 T S G H P V N P L L G A K V L P G E T D I A L P G P L P F I Query_1 331 ACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATC 420 2 130 .......................................................................................... 219 0 298 .......................................................................................... 387 L S R T Y S S Y R T K T P A P V G S L G P G W K M P A D I R Query_1 421 CTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGC 510 2 220 .......................................................................................... 309 2 3275 ....G.T..T....CA..TG....A..--.........G.. 3313 2 309 ............ 298 0 388 ..............................................C........................................... 477 0 477 ............ 466 L Q L R D N T L I L S D N G G R S L Y F E H L F P G E D G Y Query_1 511 TTACAGCTGCGCGATAACACACTGATACTCAGTGATAACGGCGGCAGAAGCCTGTATTTTGAGCACCTGTTTCCCGGTGAGGACGGTTAC 600 2 310 .......................................................................................... 399 0 478 ..........N............................................................................... 567 S R S E S L W L V R G G V A K L D E G H R L A A L W Q A L P Query_1 601 AGCCGCAGCGAGTCACTGTGGCTGGTGCGCGGCGGCGTGGCGAAACTGGATGAAGGTCACCGGCTGGCCGCACTCTGGCAGGCGCTGCCG 690 2 400 .......................................................................................... 489 0 568 .......................................................................................... 657 E E L R L S P H R Y L A T N S P Q G P W W L L G W C E R V P Query_1 691 GAAGAACTCCGCTTAAGTCCGCATCGTTATCTGGCGACAAACAGTCCGCAGGGGCCGTGGTGGCTGCTCGGTTGGTGTGAGCGGGTGCCG 780 2 490 .......................................................................................... 579 0 658 ......................................N................................................... 747 E A D E V L P A P L P P Y R V L T G L V D R F G R T Q T F Query_1 781 GAAGCGGATGAGGTGCTGCCTGCGCCGCTGCCGCCGTACCGGGTACTGACCGGGCTGGTGGACCGC-TTCGGGCGCACACAGACGTTCCA 869 2 580 ..................................................................-....................... 668 2 1663 ......-....... 1675 0 748 .....N.....N...............................N..................N...T..............T........ 837 H R E A A G E F S G E I T G V T D G A W R H F R L V L T T Query_1 870 CCGCGAAGCCG-CCGGTGAA-TTCAGCGGCGAAATCACCGGCGTGACGGA-TGGTGCCTGGCGTCAC--TTCCGGCTGGTACTGACCACG 954 2 669 ...........-........-.............................-................--..................... 753 0 838 ...........C........T...T.................T....N..T...............NTT...T..T..N...... 922 Q A Q R A E E A R Q Q A I S G G T E P S A F P D T L P G Y T Query_1 955 CAGGCGCAGCGGGCAGAAGAAGCCCGGCAGCAGGCCATTTCCGGCGGGACGGAACCGTCCGCTTTTCCTGATACCCTGCCGGGTTACACC 1044 2 754 .......................................................................................... 843 E Y G R D N G I R L S A V W L T H D P E Y P E N L P A A P L Query_1 1045 GAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTG 1134 2 844 .......................................................................................... 933 2 1312 .............. 1299 V R Y G W T P R G E L A V V Y D R S G K Q V R S F T Y D D K Query_1 1135 GTGCGCTATGGCTGGACGCCACGCGGCGAACTGGCGGTGGTGTATGACCGTAGTGGCAAACAGGTGCGCAGCTTTACTTACGATGATAAA 1224 2 934 .......................................................................................... 1023 Y R G R M V A H R H T G R P E I R Y R Y D S D G R V T E Q L Query_1 1225 TACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGGCCGGAAATCCGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTA 1314 2 1024 .......................................................................................... 1113 N P A G L S Y T Y Q Y E K D R I T I T D S L D R R E V L H T Query_1 1315 AACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACG 1404 2 1114 .......................................................................................... 1203 Q G E A G L K R V V K K E H A D G S V T Q S Q F D A V G R L Query_1 1405 CAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACGCAGAGTCAGTTTGACGCCGTGGGCAGGCTC 1494 2 1204 .......................................................................................... 1293 R A Q T D A A G R T T E Y S P D V V T G L I T R I T T P D G Query_1 1495 AGGGCACAGACGGATGCCGCAGGCAGGACAACAGAGTACAGCCCGGATGTGGTGACGGGCCTCATCACGCGCATAACCACGCCGGATGGC 1584 2 1294 .......................................................................................... 1383 2 874 .............. 861 R A S A F Y Y N H H N Q L T S A T G P D G L E L R R E Y D E Query_1 1585 AGGGCATCGGCGTTTTACTATAACCACCACAACCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAATTGCGCCGGGAATATGATGAA 1674 2 1384 .......................................................................................... 1473 2 3027 ..G..A.............. 3046 2 3505 ............ 3516 L G R L I Q E T A P D G D I T R Y R Y D N P H S D L P C A T Query_1 1675 TTGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACAGTGACTTACCCTGCGCAACG 1764 2 1474 .......................................................................................... 1563 2 3517 .G.... 3522 E D A T G S R K T M T W S R Y G Q L L S F T D C S G Y V T R Query_1 1765 GAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACCGACTGTTCCGGTTATGTAACCCGT 1854 2 1564 .......................................................................................... 1653 2 3581 ....-............T.. 3563 Y D H D R F G Q M T A V H R E E G L S Q Y R A Y D S R G Q L Query_1 1855 TATGACCATGACCGCTTCGGGCAGATGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTACCGCGCATACGACAGCCGTGGACAGTTA 1944 2 1654 .......................................................................................... 1743 2 640 ............. 652 2 1695 ............ 1684 I A V K D T Q G H E T R Y E Y N I A G D L T A V I A P D G S Query_1 1945 ATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGC 2034 2 1744 .......................................................................................... 1833 R N G T Q Y D A W G K A V R T T Q G G L T R S M E Y D A A G Query_1 2035 AGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTGACGCGCAGTATGGAATACGATGCTGCCGGA 2124 2 1834 .......................................................................................... 1923 R V I R L T S E N G S H T T F R Y D V L D R L I Q E T G F D Query_1 2125 CGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCACCTTCCGTTACGATGTACTTGACCGGCTGATACAGGAAACCGGCTTTGAC 2214 2 1924 .......................................................................................... 2013 G R T Q R Y H H D L T G K L I R S E D E G L V T H W H Y D E Query_1 2215 GGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTATCCGCAGCGAGGATGAGGGTCTGGTCACCCACTGGCACTATGACGAA 2304 2 2014 .......................................................................................... 2103 A D R L T H R T V K G E T A E R W Q Y D E R G W L T D I S H Query_1 2305 GCAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCAT 2394 2 2104 .......................................................................................... 2193 I S E G H R V A V H Y R Y D E K G R L T G E R Q T V H H P Q Query_1 2395 ATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAG 2484 2 2194 .......................................................................................... 2283 T E A L L W Q H E T R H A Y N A Q G L A N R C I P D S L P A Query_1 2485 ACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCC 2574 2 2284 .......................................................................................... 2373 V E W L T Y G S G Y L A G M K L G D T P L V E Y T R D R L H Query_1 2575 GTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCAC 2664 2 2374 .......................................................................................... 2463 R E T L R S F G R Y E L T T A Y T P A G Q L Q S Q H L N S L Query_1 2665 CGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTTATACCCCTGCCGGGCAGTTACAGAGCCAGCACCTGAACAGCCTG 2754 2 2464 .......................................................................................... 2553 L S D R D Y T W N D N G E L I R I S S P R Q T R S Y S Y S T Query_1 2755 CTGTCTGACCGCGATTACACCTGGAACGACAACGGCGAACTCATCCGCATCAGCAGCCCGCGCCAGACCCGGAGTTACAGCTACAGCACC 2844 2 2554 .......................................................................................... 2643 T G R L T G V H T T A A N L D I R I P Y A T D P A G N R L P Query_1 2845 ACCGGCAGGCTGACCGGCGTTCACACCACCGCAGCGAATCTGGATATCCGCATCCCGTATGCCACAGACCCGGCAGGTAACCGCCTGCCC 2934 2 2644 .......................................................................................... 2733 2 2826 ............ 2837 D P E L H P D S T L S M W P D N R I A R D A H Y L Y R Y D R Query_1 2935 GACCCGGAGCTGCACCCGGACAGCACCCTCAGCATGTGGCCGGATAACCGTATCGCCCGTGACGCGCACTATCTTTACCGGTATGACCGT 3024 2 2734 .......................................................................................... 2823 H G R L T E K T D L I P E G V I R T D D E R T H R Y H Y D S Query_1 3025 CACGGCAGGCTGACAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGGACTCACCGGTACCATTACGACAGT 3114 2 2824 .......................................................................................... 2913 2 2646 ............ 2657 Q H R L V H Y T R T Q Y E E P L V E S R Y L Y D P L G R R V Query_1 3115 CAGCACCGGCTGGTGCACTACACGCGGACACAATATGAAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTG 3204 2 2914 .......................................................................................... 3003 A K R V W R R E R D L T G W M S L S R K P Q V T W Y G W D G Query_1 3205 GCAAAACGGGTGTGGCGGCGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGC 3294 2 3004 .......................................................................................... 3093 2 1432 ..C..G.............. 1451 D R L T T I Q N D R S R I Q T I Y Q P G S F T P L I R V E T Query_1 3295 GACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACT 3384 2 3094 .......................................................................................... 3183 A T G E L A K T Q R R S L A D A L Q Q S G G E D G G S V V F Query_1 3385 GCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTC 3474 2 3184 .......................................................................................... 3273 P P V L V Q M L D R L E S E I L A D R V S E E S R R W L A Query_1 3475 CCGCCGGTGCTGGTGCAGATGCTCGACC--GGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCAT 3562 2 3274 ............................--............................................................ 3361 2 254 ....C.C..C....GG..GC....G..CC.........A.. 294 S C G L T V E Q M Q N Q M D P V Y T P A R K I H L Y H C D H Query_1 3563 CGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATC 3652 2 3362 .......................................................................................... 3451 R G L P L A L I S T E G A T A W C A E Y D E W G N L L N E E Query_1 3653 GCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGA 3742 2 3452 .......................................................................................... 3541 2 1462 .............T.... 1479 1 848 ...N.............AG 830 N P H Q L Q Q L I R L P G Q Q Y D E E S G L Y Y N R H R Y Query_1 3743 ACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGG-GGCAGCAGTATGATGAGGA-GTCCGGCCTGTATTACAACCGCCACCGCTATTA 3830 2 3542 ....................................-...................-................................. 3629 2 1588 ..T............T.... 1569 1 829 ...........-..............N.........-...................A.......N......................... 742 Y D P L Q G R Y I T Q D P I G L K G G W N L Y G Y Q L N P I Query_1 3831 TGACCCGCTGCAGGGG-CGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATGGATATCAATTGAATCCGATAT 3919 2 3630 ................-......................................................................... 3718 1 741 ................G..........N.............................N................................ 652 S D I D P L G L S M W E D A K S G A C T N G L C G T L S A M Query_1 3920 CAGACATCGACCCCCTGGGTTTATCTATGTGGGAGGATGCAAAATCGGGGGCATGTACTAATGGTCTTTGCGGCACACTATCCGCTATGA 4009 2 3719 .......................................................................................... 3808 1 651 ..............N..............N............................................................ 562 I G P D K F D S I D S T A Y D A L N K I N S Q S I C E D K E Query_1 4010 TAGGTCCAGATAAATTTGATTCTATAGATAGCACCGCATATGACGCCTTAAATAAAATAAATAGCCAATCTATTTGCGAAGATAAAGAGT 4099 2 3809 .......................................................................................... 3898 1 561 ...............................................................N.......................... 472 F A G L I C K D N S G R Y F S T A P N R G E R K G S Y P F N Query_1 4100 TCGCTGGTTTAATATGTAAGGATAATAGTGGCAGATATTTCTCAACAGCACCTAACCGAGGAGAAAGAAAAGGATCATATCCATTCAATA 4189 2 3899 .......................................................................................... 3988 1 471 .......................................................................................... 382 S P C P N G T E K V S A Y H T H G A D S H G E Y W D E I F S Query_1 4190 GCCCTTGCCCTAATGGTACTGAGAAAGTATCAGCTTATCATACTCATGGTGCAGATAGTCATGGAGAATATTGGGACGAAATATTTTCAG 4279 2 3989 .......................................................................................... 4078 1 381 ......N................................................................................... 292 G K D E K I V K S K D N N I K S F Y L G T P S G N F K A I D Query_1 4280 GTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATA 4369 2 4079 .......................................................................................... 4168 1 291 .......................................................................................... 202 N H G K E I T N R K G L P N V C R V H G N M * H W K W I T D Query_1 4370 ACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAACATTGGAAGTGGATAACGGATC 4459 2 4169 ................................................................. 4233 1 201 .......................................................................................... 112 P N S S S V D K L A A A L E H H H H H H * D P A A N K A R Query_1 4460 CGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGA-GATCCGGCTGCTAAC-AAAGCCCGAAA 4547 1 111 ..............................................................G...............A........... 22 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 27507480 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 6,008 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96111.102 (IDP96111.1-809.F..pMCSG68)
MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAHPGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEGVLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLDNDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLFLPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAETSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLHLIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRLLQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMGNSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDGRYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALLVDIDELVQRCPQLVQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVLNWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLDIYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATNPTIAQGRVPLVLELLSPAQRPLQITRDLSDFWKGAYREVQKEMKGRYPKHVWPDDPANTAPTRRTKKYS
GTGTCGTCGTTGCCCGTTGCTGCCGTCTTACCTGAATTACTTACCGCTCTCGATTGTGCGCCGCAGGTATTATTAAGTGCGCCGACCGGGGCCGGGAAATCAACCTGGCTGCCGCTGCAACTGCTGGCGCATCCCGGCATTAACGGGAAAATTATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTACCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAGCGGTGTTGGACTGGTGATCCTTGATGAATTTCATGAGCGCAGCTTGCAGGCGGATTTGGCGTTGGCGCTGTTACTCGATGTGCAACAAGGTCTGCGTGATGACCTTAAACTGCTGATTATGTCGGCTACCCTGGACAACGACCGCTTGCAGCAAATGCTGCCAGAAGCGCCTGTCGTCATCTCAGAAGGGCGCTCGTTTCCGGTTGAACGCCGTTATTTACCGCTGCCCGCGCATCAGCGTTTTGACGATGCCGTTGCGGTAGCCACCGCTGAAATGCTGCGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCAATGCTGGTTAGCGCGAAGAACGACGACGAAGCTGCTACCGCGGCAAAAATTGCCGCCATTCTCGAAGAGCCGCCACGGATGGGCAATAGTGACCTGGGCGTGGCGTTTTCGCGCAATCAACCAGCCTGGCAGCAACGTAGTCAGCAACTGTTAAAACGCTTAAACGTACGTGGCGGTGAGGCAGACAGTTCGCTTATCGCGCCGCTACTTGCCGGGGCGTTTGCCGATCGCATTGCTCGTCGCCGTGGGCAAGATGGACGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGGGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGACGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAGCGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGCCAGCGGTTGATGATGAAAGTTTATTGGCAGCGCTGGAAACGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,795 total letters Query= IDP96111.102 Length=2832 Score E Sequences producing significant alignments: (Bits) Value wt 4378 0.0 09A_A3_IDP96111.102_T7term.seq 1943 0.0 03A_A3_IDP96111.102_T7short.seq 1673 0.0 Q F P S R N N F V * L * E G D I H M H H H H H H S S G V D Query_1 55 CAATTCCCCTC-TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCT 143 0 16 ..T........C.............................................................................. 105 L W S H P Q F E K G T E N L Y F Q S N A V S S L P V A A V L Query_1 144 GTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGTGTCGTCGTTGCCCGTTGCTGCCGTCTTACC 233 2 1 ................................ 32 0 106 .......................................................................................... 195 P E L L T A L D C A P Q V L L S A P T G A G K S T W L P L Q Query_1 234 TGAATTACTTACCGCTCTCGATTGTGCGCCGCAGGTATTATTAAGTGCGCCGACCGGGGCCGGGAAATCAACCTGGCTGCCGCTGCAACT 323 2 33 .......................................................................................... 122 0 196 .......................................................................................... 285 L L A H P G I N G K I I L L E P R R L A A R N V A Q R L A E Query_1 324 GCTGGCGCATCCCGGCATTAACGGGAAAATTATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCT 413 2 123 .......................................................................................... 212 0 286 .......................................................................................... 375 L L N E K P G D T V G Y R M R A Q N C V G P N T R L E V V T Query_1 414 GCTTAACGAAAAGCCAGGCGATACCGTTGGCTACCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGA 503 2 213 .......................................................................................... 302 0 376 .......................................................................................... 465 E G V L T R M I Q R D P E L S G V G L V I L D E F H E R S L Query_1 504 AGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAGCGGTGTTGGACTGGTGATCCTTGATGAATTTCATGAGCGCAGCTTGCA 593 2 303 .......................................................................................... 392 0 466 .......................................................................................... 555 Q A D L A L A L L L D V Q Q G L R D D L K L L I M S A T L D Query_1 594 GGCGGATTTGGCGTTGGCGCTGTTACTCGATGTGCAACAAGGTCTGCGTGATGACCTTAAACTGCTGATTATGTCGGCTACCCTGGACAA 683 2 393 .......................................................................................... 482 0 556 .......................................................................................... 645 N D R L Q Q M L P E A P V V I S E G R S F P V E R R Y L P L Query_1 684 CGACCGCTTGCAGCAAATGCTGCCAGAAGCGCCTGTCGTCATCTCAGAAGGGCGCTCGTTTCCGGTTGAACGCCGTTATTTACCGCTGCC 773 2 483 .......................................................................................... 572 0 646 .......................................................................................... 735 P A H Q R F D D A V A V A T A E M L R Q E S G S L L L F Query_1 774 CGCGCATCAGCG-TTTTGACGATG-CCGTTGCGGTAGCCACCGCTGAAATGC-TGCGTCAGGAAAGC-GGATCATTAC-TGTTA-TTTTT 857 2 573 ............-...........-...........................-..............-..........-.....-..... 656 0 736 ............T...........C..................N........T..............G..........T.....T..... 825 L P G V G E I Q R V Q E Q L A S R I G S D V L L C P L Y G Query_1 858 ACCTGGCGTCGGAG-AAATTCAGCGTGTGCAGG-AACAACTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTG-CCCGC-TGTATGGCG 943 2 657 ..............-..................-........................................-.....-......... 742 1 808 ........... 798 0 826 ..............A..................A.........................N..............C.....T.......N. 915 A L S L N D Q R K A I L P A P Q G M R K V V L A T N I A Query_1 944 CG-TTGTCGCTGAACGATCAG-CGAAAAGCGATCC-TCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGG-CGACCAATA-TTGCTGA 1028 2 743 ..-..................-.............-....................................-.........-....... 827 1 639 ........... 629 0 916 ..T.....N............N.N....N......T.............................N......N.........N.N..... 1005 E T S L T I E G I R L V V D C A Q E R V A R F D P R T G L Query_1 1029 AACCAGTTTAACCATTGAAGGTATTCGTCTGG--TGGTGGATT-GTGCCCAGGAGCGTGTGG-CGCGTTTTGATCCGCGCACGGGGCTTA 1114 2 828 ................................--.........-..................-........................... 913 0 1006 ..-.N.........N......N.N.....N..GTG..N...N.G........A...NG....N........ 1075 T R L I T Q R V S Q A S M T Q R A G R A G R L E P G I S L H Query_1 1115 CGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATT 1204 2 914 .......................................................................................... 1003 L I A K E Q A E R A A A Q S E P E I L Q S D L S G L L M E L Query_1 1205 TAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATGGAATTAC 1294 2 1004 .......................................................................................... 1093 2 1039 ............ 1028 L Q W G C S D P A Q M S W L D Q P P V V N L L A A K R L L Q Query_1 1295 TGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAA 1384 2 1094 .......................................................................................... 1183 M L G A L E G E R L S A Q G Q K M A A L G N D P R L A A M L Query_1 1385 TGCTGGGGGCACTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCAATGCTGG 1474 2 1184 .......................................................................................... 1273 2 1973 ............ 1984 1 586 ............ 575 V S A K N D D E A A T A A K I A A I L E E P P R M G N S D L Query_1 1475 TTAGCGCGAAGAACGACGACGAAGCTGCTACCGCGGCAAAAATTGCCGCCATTCTCGAAGAGCCGCCACGGATGGGCAATAGTGACCTGG 1564 2 1274 .......................................................................................... 1363 G V A F S R N Q P A W Q Q R S Q Q L L K R L N V R G G E A Query_1 1565 GCGTGGCGTTTTCGCGCAAT-CAACCAGCCTGGCAGCAACGTA-GTCAGCAACTGTTAAAACGCTTAAACGTA-CGTGG-CGGTGAGGCA 1650 2 1364 ....................-......................-.............................-.....-.......... 1449 1 1181 .....T..TN.............-....A.....GCNN.N.N.NN--...A..C....A.....G.......... 1110 D S S L I A P L L A G A F A D R I A R R R G Q D G R Y Q L Query_1 1651 GACAGTTCGCTTATCGCGCCGCTACTTGCCGG-GGCGTTTGCCGATCGCATTGCTCGTCGCCGTGGGCAAGATGGACGCTATCAACTGGC 1739 2 1450 ................................-......................................................... 1538 1 1109 .N..AG....NNTNN......N..........N......G.............................-.................... 1021 A N G M G A M L D A N D A L S R H E W L I A P L L L Q G S A Query_1 1740 AAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGGGCAGCGCCTC 1829 2 1539 .......................................................................................... 1628 1 1020 ...........A.............................................................................. 931 S P D A R I L L A L L V D I D E L V Q R C P Q L V Q Q S D T Query_1 1830 GCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGT 1919 2 1629 .......................................................................................... 1718 1 930 .......................................................................................... 841 V E W D D A Q G T L K A W R R L Q I G Q L T V K V Q P L A K Query_1 1920 GGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACC 2009 2 1719 .......................................................................................... 1808 2 658 ........... 668 1 840 .......................................................................................... 751 P S E D E L H Q A M L N G I R D K G L S V L N W T A E A E Q Query_1 2010 GTCAGAAGACGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAGCGGAACAGCT 2099 2 1809 .......................................................................................... 1898 1 750 .......................................................................................... 661 L R L R L L C A A K W L P E Y D W P A V D D E S L L A A L E Query_1 2100 ACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGCCAGCGGTTGATGATGAAAGTTTATTGGCAGCGCTGGAAAC 2189 2 1899 .......................................................................................... 1988 2 1232 ............ 1243 1 660 .......................................................................................... 571 1 556 ........... 546 T W L L P H M T G V H S L R G L K S L D I Y Q A L R G L L D Query_1 2190 GTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTG 2279 2 1989 .......................................................................................... 2078 2 1938 ........... 1948 1 570 .......................................................................................... 481 W G M Q Q R L D S E L P A H Y T V P T G S R I A I R Y H E D Query_1 2280 GGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAA 2369 2 2079 .......................................................................................... 2168 1 480 .......................................................................................... 391 N P P A L A V R M Q E M F G E A T N P T I A Q G R V P L V L Query_1 2370 CCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGA 2459 2 2169 .......................................................................................... 2258 1 390 .......................................................................................... 301 E L L S P A Q R P L Q I T R D L S D F W K G A Y R E V Q K E Query_1 2460 GTTGCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGAT 2549 2 2259 .......................................................................................... 2348 1 300 .......................................................................................... 211 M K G R Y P K H V W P D D P A N T A P T R R T K K Y S * H W Query_1 2550 GAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAACATTGGAA 2639 2 2349 ............................................................................... 2427 1 210 .......................................................................................... 121 K W I T D P N S S S V D K L A A A L E H H H H H H * D P A A Query_1 2640 GTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGA-GATCCGGCTGCTA 2728 1 120 ............................................................................G............. 31 N K A R K E A E L A A A T A Query_1 2729 ACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGA 2771 2 1990 ............. 2002 2 607 ........... 617 1 30 .............A.... 13 1 569 ............. 557 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 13354360 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 4,795 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96135.102 (IDP96135.1-516.F..pMCSG68)
MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQLPAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLLTILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHANIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQMRFSQ
ATGCCCACTTCTCATGAAAATGCACTGCAACAACGTTGCCAGCAAATTGTCACCAGCCCAGTGCTTAGCCCGGAGCAGAAGCGCCATTTTCTGGCACTGGAAGCAGAAAACAATCTGCCTTACCCACAGCTTCCTGCCGAAGCCCGCCGCGCGCTGGATGAAGGTGTAATCTGCGATATGTTTGAAGGTCATGCGCCGTACAAACCGCGCTATGTCTTACCCGATTACGCCCGTTTTCTGGCGAACGGTTCCGAATGGCTGGAGCTGGAAGGCGCGAAAGATCTTGATGACGCACTCTCTCTGCTGACCATTCTTTACCACCACGTACCGTCGGTCACATCGATGCCGGTCTACCTGGGGCAACTGGATGCGTTGTTGCAACCGTATGTTAGAATTCTAACACAAGACGAGATCGATGTTCGAATAAAACGTTTCTGGCGTTACCTCGACAGAACCCTGCCAGACGCCTTTATGCACGCCAATATCGGCCCGTCTGATTCGCCCATTACCCGCGCGATCTTACGTGCAGATGCAGAGTTGAAGCAGGTTTCACCGAACCTGACCTTTATCTACGATCCTGAAATCACCCCTGATGACCTGCTGCTGGAAGTGGCGAAGAACATCTGTGAATGTAGCAAACCGCACATCGCCAACGGTCCGGTGCATGATAAAATTTTCACAAAAGGGGGCTACGGGATTGTGAGCTGTTACAACTCACTGCCGCTGGCGGGTGGTGGCAGCACGCTGGTACGCCTTAACCTGAAAGCCATTGCCGAGCGCAGCGAATCGCTGGATGACTTCTTTACGCGCACTCTACCGCACTACTGCCAGCAGCAGATCGCCATCATCGATGCGCGGTGTGAATTCCTCTATCAACAATCACACTTCTTTGAGAATAGCTTCCTGGTGAAAGAAGGGCTGATTAACCCTGAACGTTTTGTGCCAATGTTTGGCATGTATGGGCTGGCGGAAGCGGTTAACTTGCTGTGTGAAAAAGAAGGGATTGCCGCGCGCTACGGTAAAGAAGCCGCCGCAAATGAAGTAGGTTATCGCATCAGCGCGCAACTGGCGGAGTTTGTCGCCAATACCCCCGTGAAATATGGCTGGCAAAAACGCGCCATGTTACACGCACAGTCGGGGATCAGTTCCGATATCGGCACCACGCCGGGCGCGCGTTTGCCGTATGGCGATGAGCCAGATCCGATCACCCATCTGCAAACTGTCGCCCCGCATCATGCTTATTATTATTCCGGCATCAGCGACATTCTGACGCTCGACGAAACCATCAAACGTAACCCGCAGGCACTGGTACAGCTTTGCCTCGGTGCCTTTAAAGCCGGAATGCGTGAATTTACCGCCAATGTCAGCGGTAACGATCTGGTTCGCGTTACCGGTTATATGGTGCGTTTGTCGGATTTAGAAAAATATCGCGCCGAAGGTTCACGCACCAACACCACCTGGCTGGGCGAAGAAGCCGCACGCAACACTCGTATTCTGGAACGCCAGCCGCGCGTGATAAGCCATGAACAGCAGATGCGCTTTAGTCAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,103 total letters Query= IDP96135.102 Length=1953 Score E Sequences producing significant alignments: (Bits) Value wt 2792 0.0 09B_B3_IDP96135.102_T7term.seq 1873 0.0 03B_B3_IDP96135.102_T7short.seq 1804 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 60 CCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTC 149 0 24 ...T.......-.............................................................................. 112 S H P Q F E K G T E N L Y F Q S N A M P T S H E N A L Q Q R Query_1 150 TCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGCCCACTTCTCATGAAAATGCACTGCAACAACGTTG 239 2 1 ...................................... 38 1 1146 ........... 1136 0 113 .......................................................................................... 202 C Q Q I V T S P V L S P E Q K R H F L A L E A E N N L P Y P Query_1 240 CCAGCAAATTGTCACCAGCCCAGTGCTTAGCCCGGAGCAGAAGCGCCATTTTCTGGCACTGGAAGCAGAAAACAATCTGCCTTACCCACA 329 2 39 .......................................................................................... 128 0 203 .......................................................................................... 292 Q L P A E A R R A L D E G V I C D M F E G H A P Y K P R Y V Query_1 330 GCTTCCTGCCGAAGCCCGCCGCGCGCTGGATGAAGGTGTAATCTGCGATATGTTTGAAGGTCATGCGCCGTACAAACCGCGCTATGTCTT 419 2 129 .......................................................................................... 218 0 293 .......................................................................................... 382 0 956 ........... 966 L P D Y A R F L A N G S E W L E L E G A K D L D D A L S L L Query_1 420 ACCCGATTACGCCCGTTTTCTGGCGAACGGTTCCGAATGGCTGGAGCTGGAAGGCGCGAAAGATCTTGATGACGCACTCTCTCTGCTGAC 509 2 219 .......................................................................................... 308 0 383 .......................................................................................... 472 T I L Y H H V P S V T S M P V Y L G Q L D A L L Q P Y V R I Query_1 510 CATTCTTTACCACCACGTACCGTCGGTCACATCGATGCCGGTCTACCTGGGGCAACTGGATGCGTTGTTGCAACCGTATGTTAGAATTCT 599 2 309 .......................................................................................... 398 2 402 ............ 391 0 473 .......................................................................................... 562 0 566 ............ 555 L T Q D E I D V R I K R F W R Y L D R T L P D A F M H A N I Query_1 600 AACACAAGACGAGATCGATGTTCGAATAAAACGTTTCTGGCGTTACCTCGACAGAACCCTGCCAGACGCCTTTATGCACGCCAATATCGG 689 2 399 .......................................................................................... 488 2 390 .... 387 0 563 .......................................................................................... 652 0 554 .... 551 G P S D S P I T R A I L R A D A E L K Q V S P N L T F Query_1 690 CCCGTCTGATTCGCCC-ATTACCCGCGCGA---TCTTACGTGCAGATGCAGA-GTTGAAG-CAGGTTTCACCGA--ACCTGACC-TTTAT 770 2 489 ................-.............---...................-.......-.............--........-..... 569 1 1215 ....NNN...N....C.NNN......N.NNTT.......N...N.......A.......G...N....N....GA........C..... 1127 0 653 ................-.............---...................-.......-.............--........-..... 733 I Y D P E I T P D D L L L E V A K N I C E C S K P H Query_1 771 -CTACGAT-CCTG-AAA--TCA-CCCCTGATGACC-TGCTGCTGGAA-GTGGCG--AAGAACA--TCTGTGAATGT-AGCAAACCGCACA 847 2 570 -.......-....-...--...-............-...........-......--.......--...........-............. 646 1 1126 TN....NNN....-G..AT...N.........N..C...........A....NNGA......CAT...........N.......G..... 1038 0 734 -.......-....A...--...-............-...........-......A-.......--...........-............. 812 I A N G P V H D K I F T K G G Y G I V S C Y N S L P L A Query_1 848 T-CGCCAACGG-TCC-GGTGCATGATAAAATTTTCACAAAA-GGGGGCTACGGG-ATTGTGAGCTGTTACAACTCACTG-CCGCTGGCGG 931 2 647 .-.........-...-.........................-............-........................-.......... 730 1 1037 .T.........N...-.................N.......A............G........................-.......... 950 0 813 .-.........-...N.........................-............-........................C.......N.. 898 G G G S T L V R L N L K A I A E R S E S L D D F F T R T L Query_1 932 GTGGTGGCAGCACGCTGGTACGCCTTAACCTG-AAAGCCATTGCCGAGCGCAGCGAATCGCTGG-ATG-ACTTCTTTACGCGCACTCTAC 1018 2 731 ................................-...............................-...-..................... 817 1 949 ................................-........-......................G...-..................... 863 0 899 ................................A..........-........N...TN......-...A............NAN...... 986 P H Y C Q Q Q I A I I D A R C E F L Y Q Q S H F F E N S F Query_1 1019 CGCACTACTGCCAGCAGCAG-ATCGCCATCATCGATGCGCGGT-GTGAA--TTCCTCTATCAACAATCACACTTCTTTGAGAATAGCTTC 1104 2 818 ....................-......................-.....--....................................... 903 1 862 .......T........A...-......................-.....--....................................... 777 0 987 .......T........ATN.A......N...............G.....AT.C...........-...N..N.................. 1075 L V K E G L I N P E R F V P M F G M Y G L A E A V N L L Query_1 1105 C--TGGTGAAAG-AAGGGCTGATTAACCCTGAACGTTTTGTGCCAATGTTTGGCATGTATGGGCTGGCGGAAGCGGTTAACTT--GCTGT 1189 2 904 .--.........-......................................................................--..... 988 1 776 .--.........-......................................................................--..... 692 0 1076 .CTG........N...N.........................N.-.....G.NNN..N......CN..C.GNNN.........TGC...G 1164 C E K E G I A A R Y G K E A A A N E V G Y R I S A Q L A E F Query_1 1190 GTGAAAAAGAAGGGATTGCCGCGCGCTACGGTAAAGAAGCCGCCGCAAATGAAGTAGGTTATCGCATCAGCGCGCAACTGGCGGAGTTTG 1279 2 989 .......................................................................................... 1078 1 691 .......................................................................................... 602 0 1165 C.......A..A.. 1178 V A N T P V K Y G W Q K R A M L H A Q S G I S S D I G T T P Query_1 1280 TCGCCAATACCCCCGTGAAATATGGCTGGCAAAAACGCGCCATGTTACACGCACAGTCGGGGATCAGTTCCGATATCGGCACCACGCCGG 1369 2 1079 .......................................................................................... 1168 1 601 .......................................................................................... 512 G A R L P Y G D E P D P I T H L Q T V A P H H A Y Y Y S G I Query_1 1370 GCGCGCGTTTGCCGTATGGCGATGAGCCAGATCCGATCACCCATCTGCAAACTGTCGCCCCGCATCATGCTTATTATTATTCCGGCATCA 1459 2 1169 .......................................................................................... 1258 1 511 .......................................................................................... 422 S D I L T L D E T I K R N P Q A L V Q L C L G A F K A G M R Query_1 1460 GCGACATTCTGACGCTCGACGAAACCATCAAACGTAACCCGCAGGCACTGGTACAGCTTTGCCTCGGTGCCTTTAAAGCCGGAATGCGTG 1549 2 1259 .......................................................................................... 1348 1 421 .......................................................................................... 332 E F T A N V S G N D L V R V T G Y M V R L S D L E K Y R A E Query_1 1550 AATTTACCGCCAATGTCAGCGGTAACGATCTGGTTCGCGTTACCGGTTATATGGTGCGTTTGTCGGATTTAGAAAAATATCGCGCCGAAG 1639 2 1349 .......................................................................................... 1438 1 331 .......................................................................................... 242 G S R T N T T W L G E E A A R N T R I L E R Q P R V I S H E Query_1 1640 GTTCACGCACCAACACCACCTGGCTGGGCGAAGAAGCCGCACGCAACACTCGTATTCTGGAACGCCAGCCGCGCGTGATAAGCCATGAAC 1729 2 1439 .......................................................................................... 1528 1 241 .......................................................................................... 152 Q Q M R F S Q * H W K W I T D P N S S S V D K L A A A L E H Query_1 1730 AGCAGATGCGCTTTAGTCAGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcacc 1819 2 1529 .................... 1548 1 151 .....................................................G.................................... 62 H H H H H * D P A A N K A R K E Query_1 1820 accaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAA 1866 1 61 ...........................................A... 15 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 7844672 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 4,103 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96147.102 (IDP96147.1-481.F..pMCSG68)
MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLASALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTPGENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGVFDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMTTGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAKIAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQATLTSNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDVSWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFLDSGLLQECQQEHQQVTDTQPYHCPIPKNVTPSPLK
ATGCAGGAAATCTATCGTTTTATCGACGATGCGATTGAAGCCGATCGCCAACGTTATACCGATATTGCCGATCAAATCTGGGATCATCCAGAAACACGTTTTGAAGAGTTCTGGTCAGCGGAGCATCTGGCTTCGGCGCTGGAATCTGCAGGCTTCACCGTTACCCGCAACGTAGGCAATATCCCAAATGCCTTTATTGCTTCGTTTGGTCAAGGCAAACCGGTTATCGCCCTGCTGGGAGAATATGACGCCCTGGCAGGTTTAAGTCAGCAAGCAGGTTGCGCGCAACCTACATCCGTGACGCCCGGTGAAAATGGTCACGGTTGCGGACACAATTTGCTGGGAACCGCCGCCTTTGCCGCTGCAATAGCCGTCAAGAAATGGCTGGAACAATATGGGCAAGGCGGCACGGTGCGCTTTTATGGTTGTCCTGGCGAAGAAGGCGGCTCGGGTAAAACGTTCATGGTTCGCGAGGGGGTATTTGATGATGTGGATGCGGCACTCACCTGGCACCCGGAAGCCTTTGCCGGTATGTTCAATACCCGCACGCTGGCAAACATTCAGGCATCATGGCGCTTTAAAGGGATCGCAGCACATGCCGCGAATTCCCCTCATTTGGGACGCAGCGCCCTTGATGCCGTAACGTTGATGACCACTGGCACCAACTTCCTCAACGAACATATTATTGAAAAAGCGCGCGTACACTATGCCATCACAAATAGCGGCGGGATCTCGCCCAACGTGGTCCAGGCGCAGGCAGAAGTGCTTTATCTTATCCGCGCCCCCGAAATGACCGACGTGCAGCATATTTATGATCGGGTCGCCAAAATCGCCGAAGGTGCGGCATTGATGACCGAAACCACGGTTGAATGCCGCTTCGACAAAGCCTGTTCCAGTTATCTCCCGAATCGCACCTTAGAAAATGCCATGTACCAGGCCCTATCCCATTTTGGTACCCCGGAATGGAACTCCGAAGAACTGGCTTTTGCGAAACAAATTCAGGCTACGCTCACCTCCAACGATCGGCAAAACAGTCTGAATAATATCGCCGCAACCGGTGGCGAAAACGGCAAGGTTTTTGCACTACGTCATCGTGAAACGGTACTGGCGAATGAAGTCGCTCCATATGCCGCCACCGATAACGTGCTTGCGGCATCGACTGATGTCGGCGACGTCAGTTGGAAACTGCCTGTTGCCCAGTGTTTCAGCCCCTGTTTTGCCGTCGGTACACCGCTACATACGTGGCAACTGGTTAGCCAGGGGCGAACATCTATTGCTCATAAAGGAATGCTGCTGGCGGCGAAAACTATGGCAGCAACCACAGTCAATCTCTTCCTTGATTCAGGGCTATTGCAAGAATGCCAACAAGAGCATCAGCAAGTAACGGACACGCAACCGTATCACTGCCCTATCCCGAAAAACGTGACACCGTCACCTTTAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 2 sequences; 2,617 total letters Query= IDP96147.102 Length=1848 Score E Sequences producing significant alignments: (Bits) Value wt 2603 0.0 03C_C3_IDP96147.102_T7short.seq 1788 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 58 TTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGG 147 0 19 .....T.......T............................................................................ 108 S H P Q F E K G T E N L Y F Q S N A M Q E I Y R F I D D A I Query_1 148 TCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGCAGGAAATCTATCGTTTTATCGACGATGCGATT 237 1 1 .................................... 36 0 109 .......................................................................................... 198 E A D R Q R Y T D I A D Q I W D H P E T R F E E F W S A E H Query_1 238 GAAGCCGATCGCCAACGTTATACCGATATTGCCGATCAAATCTGGGATCATCCAGAAACACGTTTTGAAGAGTTCTGGTCAGCGGAGCAT 327 1 37 .......................................................................................... 126 0 199 .......................................................................................... 288 L A S A L E S A G F T V T R N V G N I P N A F I A S F G Q G Query_1 328 CTGGCTTCGGCGCTGGAATCTGCAGGCTTCACCGTTACCCGCAACGTAGGCAATATCCCAAATGCCTTTATTGCTTCGTTTGGTCAAGGC 417 1 127 .......................................................................................... 216 0 289 ................................................N......................................... 378 K P V I A L L G E Y D A L A G L S Q Q A G C A Q P T S V T P Query_1 418 AAACCGGTTATCGCCCTGCTGGGAGAATATGACGCCCTGGCAGGTTTAAGTCAGCAAGCAGGTTGCGCGCAACCTACATCCGTGACGCCC 507 1 217 .......................................................................................... 306 1 291 ................ 276 0 379 .......................................................................................... 468 0 453 ................ 438 G E N G H G C G H N L L G T A A F A A A I A V K K W L E Q Y Query_1 508 GGTGAAAATGGTCACGGTTGCGGACACAATTTGCTGGGAACCGCCGCCTTTGCCGCTGCAATAGCCGTCAAGAAATGGCTGGAACAATAT 597 1 307 .......................................................................................... 396 0 469 .......................................................................................... 558 G Q G G T V R F Y G C P G E E G G S G K T F M V R E G V F D Query_1 598 GGGCAAGGCGGCACGGTGCGCTTTTATGGTTGTCCTGGCGAAGAAGGCGGCTCGGGTAAAACGTTCATGGTTCGCGAGGGGGTATTTGAT 687 1 397 .......................................................................................... 486 0 559 .......................................................................................... 648 D V D A A L T W H P E A F A G M F N T R T L A N I Q A S W R Query_1 688 GATGTGGATGCGGCACTCACCTGGCACCCGGAAGCCTTTGCCGGTATGTTCAATACCCGCACGCTGGCAAACATTCAGGCATCATGGCGC 777 1 487 .......................................................................................... 576 0 649 .......................................................................T.................. 738 F K G I A A H A A N S P H L G R S A L D A V T L M T T G T Query_1 778 TTTAAAGGGATCGCAGCACATGCCGCGAATTCCCCTCATTTGGGACGCAGCGCCCTTGATGCCGTAACGTTGATGACCACTGGCACC-AA 866 1 577 .......................................................................................-.. 665 0 739 ........N...........................Y..................................................N.. 828 N F L N E H I I E K A R V H Y A I T N S G G I S P N V V Q A Query_1 867 CTTCCTCAACGAACATATTATTGAAAAAGCGCGCGTACACTATGCCATCACAAATAGCGGCGGGA-TCTCGCCCAACGTGGTCCAGGCGC 955 1 666 .................................................................-........................ 754 0 829 .................................................................T....A................... 918 Q A E V L Y L I R A P E M T D V Q H I Y D R V A K I A E G Query_1 956 AGGCAGAAGTGC-TTTATC-TTATCCGCGCCCCCGAAATGACCGACGTGCAGCATATTTATGATCGGGTCGCCAAAATCGCCGAAGGTGC 1043 1 755 ............-......-...................................................................... 842 0 919 .N..........T......T........N.............G........N.N....NTGA........N..-............N... 1007 A A L M T E T T V E C R F D K A C S S Y L P N R T L E N A M Query_1 1044 GGCATTGATGACCGAAACCACGGTTGAATGCCGCTTCGACAAAGCCTGTTCCAGTTATCTCCCGAATCGCACCTTAGAAAATGCCATGTA 1133 1 843 .......................................................................................... 932 0 1008 -.N.............--.C.C....................--G...-.N................NCN..N..........G...TN. 1091 Y Q A L S H F G T P E W N S E E L A F A K Q I Q A T L T Query_1 1134 CCAGGCCCTATCCCATTTTGG-TACCCCGGAATGGAACTCCGAAGAA--CTGGCTTTTGCG---AAACAAATTCAGGCTACGCTCACCTC 1217 1 933 .....................-.........................--............---.......................... 1016 0 1092 ..-.NN.....N.........N..--.NN...........NN..A..AN.N..........CGA....... 1159 S N D R Q N S L N N I A A T G G E N G K V F A L R H R E T V Query_1 1218 CAACGATCGGCAAAACAGTCTGAATAATATCGCCGCAACCGGTGGCGAAAACGGCAAGGTTTTTGCACTACGTCATCGTGAAACGGTACT 1307 1 1017 .......................................................................................... 1106 1 1222 ........... 1212 L A N E V A P Y A A T D N V L A A S T D V G D V S W K L P V Query_1 1308 GGCGAATGAAGTCGCTCCATATGCCGCCACCGATAACGTGCTTGCGGCATCGACTGATGTCGGCGACGTCAGTTGGAAACTGCCTGTTGC 1397 1 1107 .......................................................................................... 1196 A Q C F S P C F A V G T P L H T W Q L V S Q G R T S I A H K Query_1 1398 CCAGTGTTTCAGCCCCTGTTTTGCCGTCGGTACACCGCTACATACGTGGCAACTGGTTAGCCAGGGGCGAACATCTATTGCTCATAAAGG 1487 1 1197 .......................................................................................... 1286 1 1034 ........... 1024 G M L L A A K T M A A T T V N L F L D S G L L Q E C Q Q E H Query_1 1488 AATGCTGCTGGCGGCGAAAACTATGGCAGCAACCACAGTCAATCTCTTCCTTGATTCAGGGCTATTGCAAGAATGCCAACAAGAGCATCA 1577 1 1287 .......................................................................................... 1376 Q Q V T D T Q P Y H C P I P K N V T P S P L K Query_1 1578 GCAAGTAACGGACACGCAACCGTATCACTGCCCTATCCCGAAAAACGTGACACCGTCACCTTTAAAA 1644 1 1377 ................................................................... 1443 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4735720 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:08 PM Number of letters in database: 2,617 Number of sequences in database: 2 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2