Clone ID: IDP00090.121 (IDP00090.1-133.F..pMCSG73)
MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNVKPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLREFHEKLRELLTTLNLKLEPANDFRDEPVKLTA
ATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCGAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGCTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGATACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGAATGATTTTCGTGATGAACCGGTGAAGTTAACGGCG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,110 total letters Query= IDP00090.121 Length=804 Score E Sequences producing significant alignments: (Bits) Value 07H_H9_IDP00090.121_T7term.seq 820 0.0 03H_H9_IDP00090.121_v73F.seq 774 0.0 wt 720 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 1 514 ...........A........G....------....................... 467 1 532 .....C........... 516 0 42 ...........A........G....------...................A... 89 A M T L P S G H P K S R L I K K F T A L G P Y I R E G K C E Query_1 199 GCCATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCGAA 288 1 466 .......................................................................................... 377 0 90 ............................................................N............................. 179 2 1 ....................................................................................... 87 D N R F F F D C L A V C V N V K P A P E V R E F W G W W M E Query_1 289 GATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAG 378 1 376 .......................................................................................... 287 0 180 .......N.............................................N.........N.......................... 269 2 88 .......................................................................................... 177 L E A Q E S R F T Y S Y Q F G L F D K A G D W K S V P V K D Query_1 379 CTGGAAGCGCAGGAATCCCGTTTTACCTACAGCTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGAT 468 1 286 .......................................................................................... 197 0 270 .......................................................................N.................. 359 2 178 .......................................................................................... 267 T E V V E R L E H T L R E F H E K L R E L L T T L N L K L E Query_1 469 ACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAA 558 1 196 .......................................................................................... 107 0 360 ......N...........................................T.................N..............N...... 449 2 268 .......................................................................................... 357 P A N D F R D E P V K L T A * H W K W I T D P N S S S V D K Query_1 559 CCGGCGAATGATTTTCGTGATGAACCGGTGAAGTTAACGGCGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAG 648 1 106 ......................T.......................................--..... 40 0 450 ....T....................................N..T.................--..... 516 2 358 .......................................... 399 L A A A L E H H H H H H * D P A A N K A R K E A E L A A A T Query_1 649 CTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACC 738 1 44 ........................... 18 0 512 .................N................N....T.......... 561 Query_1 739 G 739 0 562 . 562 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1629285 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:09 PM Number of letters in database: 2,110 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP00423.105 (IDP00423.25-962.P(962)..pMCSG73)
AIPAWQPLAETIYKSEHDLRKYQAIKLSNGMTVLLVSDTQAPKSLAALALPVGSLEDPDNQLGLAHYLEHMVLMGSKHFPEPGSFSEFLKKHGGSHNASTASYRTAFYLEIENDALAPAVERLADAIAEPLLDPINADRERNAVNAELTMARSRDGMRMAQVNAETLNPAHPSARFSGGNLETLRDKPDGKLHDELVSFYHRYYSANLMVGVLYSNQSLEQLAQLAADTFGRIPNRDAKVPTITVPVVTPDQTGIIIHYVPAQPRKQIKVDFRIANNSADFRSKTDTYISYLISNRSKNTLSDWLQKQGLADAINAGADPMLDRNGGVFSITVSLTDKGLAQRDVVVAAIFDYINMLHKEGIKKSYFDEIAHVLNLDFRYPSITRDMDYIEWLVDMMLRVPVAHTLDAPYLADQYDPKAIASRLAEMTPENARIWFVSPEEPHNKVAYFVDAPYQVDKIGVQRMKEWQQLGQKIALSLPALNPYIPDNFTLIKADKNITRPQNVADQPGLRVFYMPSQYFADEPKADITVAFRNPHALNSARHQVLFALTDYLAGLSLDQLSYQASIGGISFSTAPNNGLYVNAGGFTQRMPQLLTSLVSGYASFTPTEEQLVQAKSWYREQLDVAEKGKAYELAIQPAKLLSNVPYSERSERRKLLDSISVQDVLTYRDDLLKQSAIEVLAVGNMTAEQVTELTESLKKQLNLIGTTWWVGEDVIIEKTQLANMERVGSSSDAALAAVYVPTGYTEIAGMARSALLGQIIQPWFYDQLRTEEQLGYAVFSFPMSVGHQWGIGFLLQSNSKEPNYLYQRYLAFYPQAEKRLREMKPDDFEQYKQGLVNQLLQRPQTLDEEAERYRKDFNLNNFAFDSREKMIAQVKQLTANELADFFQQAVIKPQGLALLSQVKGQGQAGGFAVPEGWTTYPTTSALQATLPQKVLAP
GCAATTCCTGCGTGGCAACCTTTGGCGGAAACCATTTACAAAAGTGAACATGACTTACGTAAATATCAGGCAATAAAATTATCCAATGGTATGACGGTACTACTGGTTTCCGATACACAAGCCCCTAAATCTTTGGCGGCCTTAGCGCTACCGGTAGGGTCACTGGAAGACCCAGATAATCAGCTCGGCTTGGCCCACTACTTGGAGCATATGGTGCTCATGGGGTCCAAACATTTCCCTGAACCTGGCAGTTTTTCCGAATTCCTAAAAAAGCATGGGGGGAGCCATAATGCGAGCACGGCTTCTTACCGAACCGCTTTCTATCTTGAAATTGAAAATGATGCATTGGCACCTGCGGTTGAGCGGTTGGCGGATGCCATTGCTGAACCCTTGTTAGACCCCATTAATGCGGATCGTGAGCGTAATGCTGTCAATGCTGAATTGACGATGGCCCGTTCACGCGATGGTATGCGTATGGCGCAAGTTAATGCGGAAACGCTTAATCCGGCACATCCAAGCGCACGTTTTTCTGGTGGTAACCTTGAAACTCTTAGGGACAAGCCCGATGGCAAGCTACATGATGAATTGGTGAGCTTCTATCACCGCTACTATTCTGCCAATCTCATGGTCGGGGTGTTGTACAGCAACCAATCATTAGAGCAGCTAGCACAATTAGCCGCAGACACCTTCGGCCGTATCCCTAATAGGGATGCAAAAGTACCCACGATTACGGTCCCAGTCGTGACACCTGATCAAACGGGAATCATCATTCACTATGTTCCTGCTCAACCTCGTAAACAGATAAAAGTCGATTTCCGTATTGCGAACAATAGTGCGGATTTTCGTAGTAAAACAGATACCTATATTAGCTACTTGATCAGTAATCGCAGTAAAAACACCTTATCTGACTGGCTACAAAAACAGGGGCTGGCAGATGCAATCAATGCGGGTGCTGACCCAATGTTGGATAGAAATGGGGGGGTGTTCTCTATCACTGTCTCACTAACCGATAAGGGGCTGGCACAACGTGATGTTGTCGTCGCGGCAATTTTTGATTACATCAATATGCTGCATAAAGAGGGGATTAAAAAAAGCTATTTCGATGAAATTGCACATGTATTGAATCTCGATTTCCGTTATCCCTCTATCACCCGTGATATGGACTATATCGAATGGCTCGTGGATATGATGTTACGAGTTCCTGTTGCACATACGCTTGATGCGCCTTATCTGGCTGATCAGTATGATCCCAAAGCAATTGCATCCCGATTGGCCGAGATGACGCCTGAAAATGCCCGCATCTGGTTTGTCAGCCCTGAAGAACCGCATAACAAAGTAGCTTATTTTGTCGATGCACCTTATCAGGTCGATAAAATAGGCGTACAGCGGATGAAAGAATGGCAGCAACTGGGGCAAAAGATTGCGCTAAGTTTGCCAGCACTGAACCCGTACATTCCTGATAATTTCACTCTGATCAAAGCAGACAAGAACATTACCCGCCCACAGAACGTGGCAGACCAGCCAGGATTGCGGGTGTTTTACATGCCAAGTCAGTATTTTGCTGACGAACCCAAAGCCGACATTACGGTTGCTTTCCGCAACCCTCATGCATTGAACTCAGCTCGCCATCAGGTGCTTTTTGCCCTGACGGATTACCTTGCGGGTCTCTCACTTGATCAACTGAGTTATCAGGCCTCTATTGGTGGGATCAGTTTCTCTACCGCACCGAACAATGGCTTGTATGTTAATGCTGGTGGTTTTACACAGCGTATGCCGCAGTTACTGACATCTTTGGTTTCAGGCTATGCCAGTTTTACTCCGACAGAAGAGCAATTGGTACAGGCTAAATCTTGGTATCGTGAGCAATTAGACGTGGCAGAGAAAGGCAAAGCTTATGAGTTGGCTATTCAGCCCGCTAAGCTGCTATCGAATGTGCCTTATTCGGAGCGAAGTGAGCGACGTAAACTACTTGATAGCATCAGTGTGCAGGATGTGCTGACCTATAGAGATGATTTACTGAAACAGTCTGCGATAGAAGTTTTGGCCGTGGGCAATATGACAGCCGAACAAGTCACTGAACTCACTGAATCGCTGAAAAAACAGCTGAACTTAATCGGAACCACGTGGTGGGTCGGTGAAGATGTCATTATTGAGAAAACACAGTTGGCCAATATGGAACGGGTCGGCAGTAGTTCTGACGCGGCTTTGGCAGCAGTTTATGTCCCTACTGGCTATACAGAAATTGCTGGTATGGCACGTAGTGCCTTGTTAGGGCAGATCATTCAACCATGGTTCTATGATCAATTACGGACAGAAGAACAGCTTGGTTATGCTGTATTTTCTTTCCCAATGTCGGTTGGTCATCAATGGGGCATCGGCTTCTTACTGCAAAGTAATAGTAAGGAACCTAATTATCTTTACCAGCGATACCTCGCATTTTATCCGCAAGCTGAAAAACGCCTGCGCGAAATGAAGCCCGATGATTTTGAACAATATAAGCAGGGGCTGGTCAATCAACTATTGCAAAGGCCACAGACATTAGATGAAGAGGCAGAGCGCTACCGTAAAGACTTCAATCTCAATAATTTTGCATTTGATAGCCGTGAGAAGATGATTGCTCAAGTGAAACAGCTTACGGCTAATGAACTGGCGGATTTCTTCCAGCAAGCGGTCATTAAACCACAAGGTCTGGCACTGCTTTCTCAAGTTAAAGGTCAGGGGCAGGCCGGAGGATTCGCAGTACCGGAGGGATGGACGACTTATCCAACCACTTCCGCTTTACAGGCTACGCTGCCGCAGAAGGTATTGGCACCA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,582 total letters Query= IDP00423.105 Length=3219 Score E Sequences producing significant alignments: (Bits) Value wt 5075 0.0 04F_F10_IDP00423.105_v73F.seq 1557 0.0 08F_F10_IDP00423.105_T7term.seq 1348 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 0 43 ...........A........G....------...............N....... 90 0 27 ...C........... 41 0 28 .............. 41 A A I P A W Q P L A E T I Y K S E H D L R K Y Q A I K L S N Query_1 199 GCCGCAATTCCTGCGTGGCAACCTTTGGCGGAAACCATTTACAAAAGTGAACATGACTTACGTAAATATCAGGCAATAAAATTATCCAAT 288 2 1 ....................................................................................... 87 0 91 .......................................................................................... 180 G M T V L L V S D T Q A P K S L A A L A L P V G S L E D P D Query_1 289 GGTATGACGGTACTACTGGTTTCCGATACACAAGCCCCTAAATCTTTGGCGGCCTTAGCGCTACCGGTAGGGTCACTGGAAGACCCAGAT 378 2 88 .......................................................................................... 177 0 181 .......................................................................................... 270 N Q L G L A H Y L E H M V L M G S K H F P E P G S F S E F L Query_1 379 AATCAGCTCGGCTTGGCCCACTACTTGGAGCATATGGTGCTCATGGGGTCCAAACATTTCCCTGAACCTGGCAGTTTTTCCGAATTCCTA 468 2 178 .......................................................................................... 267 0 271 .......................................................................................... 360 K K H G G S H N A S T A S Y R T A F Y L E I E N D A L A P A Query_1 469 AAAAAGCATGGGGGGAGCCATAATGCGAGCACGGCTTCTTACCGAACCGCTTTCTATCTTGAAATTGAAAATGATGCATTGGCACCTGCG 558 2 268 .......................................................................................... 357 2 2376 ........... 2386 0 361 .......................................................................................... 450 1 504 ........... 494 V E R L A D A I A E P L L D P I N A D R E R N A V N A E L T Query_1 559 GTTGAGCGGTTGGCGGATGCCATTGCTGAACCCTTGTTAGACCCCATTAATGCGGATCGTGAGCGTAATGCTGTCAATGCTGAATTGACG 648 2 358 .......................................................................................... 447 0 451 .......................................................................................... 540 M A R S R D G M R M A Q V N A E T L N P A H P S A R F S G G Query_1 649 ATGGCCCGTTCACGCGATGGTATGCGTATGGCGCAAGTTAATGCGGAAACGCTTAATCCGGCACATCCAAGCGCACGTTTTTCTGGTGGT 738 2 448 .......................................................................................... 537 0 541 ......................................................................N................... 630 N L E T L R D K P D G K L H D E L V S F Y H R Y Y S A N L M Query_1 739 AACCTTGAAACTCTTAGGGACAAGCCCGATGGCAAGCTACATGATGAATTGGTGAGCTTCTATCACCGCTACTATTCTGCCAATCTCATG 828 2 538 .......................................................................................... 627 0 631 .......................................................................................... 720 V G V L Y S N Q S L E Q L A Q L A A D T F G R I P N R D A Query_1 829 GTCGGGGTGTTGTACAGCAACCAATCATTAGAGCAGCTAGCACAA-TTAGCCGCAGACACCTTCGGCCGTATCCCTAATAGGGATGCAAA 917 2 628 .............................................-............................................ 716 0 721 .............................................T....N....................................... 810 K V P T I T V P V V T P D Q T G I I I H Y V P A Q P R K Q Query_1 918 AGTACCCACGATTACGGTCCCAGTCGTGACACCTGATCAAACGGGAATCATCATTCACTATGTT-CCTGC-TCAACC-TCGTAAACAGAT 1004 2 717 ................................................................-.....-......-............ 803 0 811 ...............-......................-.........................C.....T......T............ 898 I K V D F R I A N N S A D F R S K T D T Y I S Y L I S N R S Query_1 1005 AAAAGTCGATTTCCGTATT-GCGAACAATAGTGCGG-ATTTTCGTAGTAAAACAGATACCTATATTAGCTACTTGATCAGTAATCGCAGT 1092 2 804 ...................-................-..................................................... 891 2 1127 ........... 1137 0 899 ...................G......N.........A.......................... 961 K N T L S D W L Q K Q G L A D A I N A G A D P M L D R N G G Query_1 1093 AAAAACACCTTATCTGACTGGCTACAAAAACAGGGGCTGGCAGATGCAATCAATGCGGGTGCTGACCCAATGTTGGATAGAAATgggggg 1182 2 892 .......................................................................................... 981 2 1013 ........... 1023 V F S I T V S L T D K G L A Q R D V V V A A I F D Y I N M L Query_1 1183 gTGTTCTCTATCACTGTCTCACTAACCGATAAGGGGCTGGCACAACGTGATGTTGTCGTCGCGGCAATTTTTGATTACATCAATATGCTG 1272 2 982 .......................................................................................... 1071 H K E G I K K S Y F D E I A H V L N L D F R Y P S I T R D M Query_1 1273 CATAAAGAGGGGATTaaaaaaaGCTATTTCGATGAAATTGCACATGTATTGAATCTCGATTTCCGTTATCCCTCTATCACCCGTGATATG 1362 2 1072 .......................................................................................... 1161 2 809 ........... 819 D Y I E W L V D M M L R V P V A H T L D A P Y L A D Q Y D P Query_1 1363 GACTATATCGAATGGCTCGTGGATATGATGTTACGAGTTCCTGTTGCACATACGCTTGATGCGCCTTATCTGGCTGATCAGTATGATCCC 1452 2 1162 .......................................................................................... 1251 K A I A S R L A E M T P E N A R I W F V S P E E P H N K V A Query_1 1453 AAAGCAATTGCATCCCGATTGGCCGAGATGACGCCTGAAAATGCCCGCATCTGGTTTGTCAGCCCTGAAGAACCGCATAACAAAGTAGCT 1542 2 1252 .......................................................................................... 1341 Y F V D A P Y Q V D K I G V Q R M K E W Q Q L G Q K I A L S Query_1 1543 TATTTTGTCGATGCACCTTATCAGGTCGATAAAATAGGCGTACAGCGGATGAAAGAATGGCAGCAACTGGGGCAAAAGATTGCGCTAAGT 1632 2 1342 .......................................................................................... 1431 L P A L N P Y I P D N F T L I K A D K N I T R P Q N V A D Q Query_1 1633 TTGCCAGCACTGAACCCGTACATTCCTGATAATTTCACTCTGATCAAAGCAGACAAGAACATTACCCGCCCACAGAACGTGGCAGACCAG 1722 2 1432 .......................................................................................... 1521 P G L R V F Y M P S Q Y F A D E P K A D I T V A F R N P H A Query_1 1723 CCAGGATTGCGGGTGTTTTACATGCCAAGTCAGTATTTTGCTGACGAACCCAAAGCCGACATTACGGTTGCTTTCCGCAACCCTCATGCA 1812 2 1522 .......................................................................................... 1611 L N S A R H Q V L F A L T D Y L A G L S L D Q L S Y Q A S I Query_1 1813 TTGAACTCAGCTCGCCATCAGGTGCTTTTTGCCCTGACGGATTACCTTGCGGGTCTCTCACTTGATCAACTGAGTTATCAGGCCTCTATT 1902 2 1612 .......................................................................................... 1701 G G I S F S T A P N N G L Y V N A G G F T Q R M P Q L L T S Query_1 1903 GGTGGGATCAGTTTCTCTACCGCACCGAACAATGGCTTGTATGTTAATGCTGGTGGTTTTACACAGCGTATGCCGCAGTTACTGACATCT 1992 2 1702 .......................................................................................... 1791 L V S G Y A S F T P T E E Q L V Q A K S W Y R E Q L D V A E Query_1 1993 TTGGTTTCAGGCTATGCCAGTTTTACTCCGACAGAAGAGCAATTGGTACAGGCTAAATCTTGGTATCGTGAGCAATTAGACGTGGCAGAG 2082 2 1792 .......................................................................................... 1881 K G K A Y E L A I Q P A K L L S N V P Y S E R S E R R K L L Query_1 2083 AAAGGCAAAGCTTATGAGTTGGCTATTCAGCCCGCTAAGCTGCTATCGAATGTGCCTTATTCGGAGCGAAGTGAGCGACGTAAACTACTT 2172 2 1882 .......................................................................................... 1971 D S I S V Q D V L T Y R D D L L K Q S A I E V L A V G N M T Query_1 2173 GATAGCATCAGTGTGCAGGATGTGCTGACCTATAGAGATGATTTACTGAAACAGTCTGCGATAGAAGTTTTGGCCGTGGGCAATATGACA 2262 2 1972 .......................................................................................... 2061 A E Q V T E L T E S L K K Q L N L I G T T W W V G E D V I Query_1 2263 GCCGAACAAGTCACTGAACTCACTGAATCGCTGAAAAAA-CAGCTGAACTTAA-TCGGAACCACGTGGTG-GGTCGGTGAAGATGTCATT 2349 2 2062 .......................................-.............-................-................... 2148 1 805 .......................A.............A................A................... 732 I E K T Q L A N M E R V G S S S D A A L A A V Y V P T G Y T Query_1 2350 ATTGAGAAAACACAGTTGGCCAATATGGAACGGGTCGGCAGTAGTTCTGACGCGGCTTTGGCAGCAGTTTATGTCCCTACTGGCTATACA 2439 2 2149 .......................................................................................... 2238 1 731 .......................................................................................... 642 E I A G M A R S A L L G Q I I Q P W F Y D Q L R T E E Q L G Query_1 2440 GAAATTGCTGGTATGGCACGTAGTGCCTTGTTAGGGCAGATCATTCAACCATGGTTCTATGATCAATTACGGACAGAAGAACAGCTTGGT 2529 2 2239 .......................................................................................... 2328 1 641 .......................................................................................... 552 Y A V F S F P M S V G H Q W G I G F L L Q S N S K E P N Y L Query_1 2530 TATGCTGTATTTTCTTTCCCAATGTCGGTTGGTCATCAATGGGGCATCGGCTTCTTACTGCAAAGTAATAGTAAGGAACCTAATTATCTT 2619 2 2329 .......................................................................................... 2418 0 392 ........... 402 1 551 .......................................................................................... 462 Y Q R Y L A F Y P Q A E K R L R E M K P D D F E Q Y K Q G L Query_1 2620 TACCAGCGATACCTCGCATTTTATCCGCAAGCTGAAAAACGCCTGCGCGAAATGAAGCCCGATGATTTTGAACAATATAAGCAGGGGCTG 2709 2 2419 .......................................................................................... 2508 1 461 .......................................................................................... 372 V N Q L L Q R P Q T L D E E A E R Y R K D F N L N N F A F D Query_1 2710 GTCAATCAACTATTGCAAAGGCCACAGACATTAGATGAAGAGGCAGAGCGCTACCGTAAAGACTTCAATCTCAATAATTTTGCATTTGAT 2799 2 2509 .......................................................................................... 2598 1 371 .......................................................................................... 282 S R E K M I A Q V K Q L T A N E L A D F F Q Q A V I K P Q G Query_1 2800 AGCCGTGAGAAGATGATTGCTCAAGTGAAACAGCTTACGGCTAATGAACTGGCGGATTTCTTCCAGCAAGCGGTCATTAAACCACAAGGT 2889 2 2599 .......................................................................................... 2688 1 281 .......................................................................................... 192 L A L L S Q V K G Q G Q A G G F A V P E G W T T Y P T T S A Query_1 2890 CTGGCACTGCTTTCTCAAGTTAAAGGTCAGGGGCAGGCCGGAGGATTCGCAGTACCGGAGGGATGGACGACTTATCCAACCACTTCCGCT 2979 2 2689 .......................................................................................... 2778 1 191 .......................................................................................... 102 L Q A T L P Q K V L A P * H W K W I T D P N S S S V D K L A Query_1 2980 TTACAGGCTACGCTGCCGCAGAAGGTATTGGCACCATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCG 3069 2 2779 .................................... 2814 1 101 ........................................................--..... 41 A A L E H H H H H H * D P A A N K A R K E Query_1 3070 GCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAG-CCCGAAAGGAA 3132 1 45 ..................C.......A... 16 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 14508262 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:09 PM Number of letters in database: 4,582 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP00510.103 (IDP00510.20-207.P(207)..pMCSG73)
VDQSNPYRLMDEAAKKTFTRLKNEQPKIKQNPDYLRTIVREELLPFVQIKYAGALVLGSYYKDATPAQREAYFNAFGKYLEQAYGQALALYHGQTYDVAPDQPLGDANIVAIRVTILDPSGRPPVRLDFQWRKNSQTGNWQAYDMIAEGVSMISTKQNEWASILRQKGVDGLTQQLLSAAKQPITLDK
GTCGATCAAAGCAACCCTTATCGTCTGATGGATGAGGCAGCGAAGAAAACCTTTACGCGTCTGAAAAATGAGCAGCCTAAGATTAAGCAAAATCCAGATTATCTCCGCACCATTGTACGCGAAGAGCTGTTGCCGTTTGTTCAGATCAAATACGCCGGCGCGTTAGTACTGGGTAGCTATTATAAAGATGCTACACCTGCACAGCGTGAAGCGTATTTCAATGCATTCGGTAAATATCTGGAGCAGGCATACGGGCAGGCATTGGCGTTGTATCACGGCCAAACTTACGATGTGGCACCAGACCAGCCTTTAGGGGATGCCAACATCGTTGCTATCCGCGTCACCATTCTCGATCCAAGTGGGCGTCCACCTGTCCGGTTAGATTTCCAATGGCGTAAAAATAGCCAAACCGGTAACTGGCAGGCTTATGACATGATCGCCGAAGGGGTGAGCATGATTAGCACCAAACAGAATGAATGGGCCTCTATCCTGCGCCAAAAGGGTGTGGATGGTTTGACCCAACAATTGCTGAGTGCGGCTAAACAGCCAATCACCTTAGATAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,807 total letters Query= IDP00510.103 Length=969 Score E Sequences producing significant alignments: (Bits) Value 04D_D10_IDP00510.103_v73F.seq 1122 0.0 08D_D10_IDP00510.103_T7term.seq 1117 0.0 wt 1018 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 0 41 ...........A........G....------....................... 88 0 24 ....C........... 39 0 26 .............. 39 1 680 ...........A........G....------....................... 633 1 698 .....C........... 682 A V D Q S N P Y R L M D E A A K K T F T R L K N E Q P K I K Query_1 199 GCCGTCGATCAAAGCAACCCTTATCGTCTGATGGATGAGGCAGCGAAGAAAACCTTTACGCGTCTGAAAAATGAGCAGCCTAAGATTAAG 288 0 89 .......................................................................................... 178 1 632 .....................................T.................................................... 543 2 1 ....................................................................................... 87 Q N P D Y L R T I V R E E L L P F V Q I K Y A G A L V L G S Query_1 289 CAAAATCCAGATTATCTCCGCACCATTGTACGCGAAGAGCTGTTGCCGTTTGTTCAGATCAAATACGCCGGCGCGTTAGTACTGGGTAGC 378 0 179 .......................................................................................... 268 1 542 .......................................................................................... 453 1 1025 ........... 1015 2 88 .......................................................................................... 177 Y Y K D A T P A Q R E A Y F N A F G K Y L E Q A Y G Q A L A Query_1 379 TATTATAAAGATGCTACACCTGCACAGCGTGAAGCGTATTTCAATGCATTCGGTAAATATCTGGAGCAGGCATACGGGCAGGCATTGGCG 468 0 269 .......................................................................................... 358 1 452 .......................................................................................... 363 2 178 .......................................................................................... 267 L Y H G Q T Y D V A P D Q P L G D A N I V A I R V T I L D P Query_1 469 TTGTATCACGGCCAAACTTACGATGTGGCACCAGACCAGCCTTTAGGGGATGCCAACATCGTTGCTATCCGCGTCACCATTCTCGATCCA 558 0 359 .......................................................................................... 448 1 362 .......................................................................................... 273 2 268 .......................................................................................... 357 S G R P P V R L D F Q W R K N S Q T G N W Q A Y D M I A E G Query_1 559 AGTGGGCGTCCACCTGTCCGGTTAGATTTCCAATGGCGTAAAAATAGCCAAACCGGTAACTGGCAGGCTTATGACATGATCGCCGAAGGG 648 0 449 .......................................................................................... 538 1 272 .......................................................................................... 183 2 358 .......................................................................................... 447 V S M I S T K Q N E W A S I L R Q K G V D G L T Q Q L L S A Query_1 649 GTGAGCATGATTAGCACCAAACAGAATGAATGGGCCTCTATCCTGCGCCAAAAGGGTGTGGATGGTTTGACCCAACAATTGCTGAGTGCG 738 0 539 .......................................................................................... 628 1 182 .......................................................................................... 93 2 448 .......................................................................................... 537 A K Q P I T L D K * H W K W I T D P N S S S V D K L A A A L Query_1 739 GCTAAACAGCCAATCACCTTAGATAAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTC 828 0 629 ...............................................--..... 680 1 92 ...............................................--..... 41 2 538 ........................... 564 E H H H H H H * D P A A N K A R K E A E L A A A T A E Q * Query_1 829 GAGcaccaccaccaccaccacTGAGATCCGGCTGCTAAC-AAAGCCC--GAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAA 915 0 676 ..............-.......--......................................... 737 1 45 ..............A.......CGA........ 13 L A * P L G A S K R V L R G F L L K Query_1 916 CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAA 969 0 738 ...................................................... 791 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2634948 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:09 PM Number of letters in database: 2,807 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP00563.103 (IDP00563.29-676.P(676)..pMCSG73)
ADTTTTQTSSKKHSTDTMVVTATGNERSSFEAPMMVTVIEGNAPTSQTAATAADMLRQVPGLTVTGSGRTNGQDVVMRGYGKQGVLTLVDGVRQGTDTGHLNSTFLDPALVKRIEIVRGPAALLYGSGALGGVIAYETVDAADMLQPGQNSGYRVYSSAATGDHSFGLGASAFGRTDDLDGILSFGTRDIGNIRQSNGFNAPNDETISNVLAKGTWQIDSIQSLSANLRYYNNSAIEPKNPQTSAPSSTNVMTNRSTIQRDAQLRYNIKPLDQEWLNATAQVYYSEVEINARPQGSAEEGREQTTEGVKLENRTRLFIESPASHLLTYGTETYKQEQTPGGATESFPQAKIRFSSGWLQDEITLRDLPVSILAGTRYDNYSGSSDGYADVDADKWSSRGAISITPTDWLMLFGSYAQAFRAPTMGEMYNDSKHFAIPIRPGLTLTNYWVPNPNLKPETNETQEYGFGLRFSDLLMAEDDLQFKVSYFDTKAKDYISTRVDMQAMTTTSVNIDQAKIWGWDASMSYKTALFNWDLAYNRTRGKNQNTDEWLDTINPDTVTSIVDVPVANSGFSVGWIGTFANRSSRVSSSTPQAGYGVNDFYVSYKGQEAFKGMTTTMLLGNVFEKEYYTPQGIPQDGRNVKFFVSYQW
GCTGATACCACAACCACTCAAACCAGCAGTAAAAAGCACAGCACCGACACCATGGTCGTCACAGCAACCGGTAATGAGCGTAGCAGTTTTGAAGCGCCAATGATGGTAACGGTCATCGAAGGGAATGCCCCTACCAGCCAAACCGCCGCGACGGCCGCAGATATGCTGCGACAAGTACCAGGGCTGACCGTCACTGGCAGCGGGCGTACCAACGGTCAAGATGTGGTGATGCGAGGTTACGGTAAACAAGGCGTGTTGACACTGGTTGATGGTGTTCGTCAGGGGACCGATACCGGTCACTTGAACTCCACTTTCCTCGATCCTGCGTTAGTCAAACGTATCGAAATCGTCCGTGGGCCAGCAGCACTGCTGTACGGCAGTGGCGCTCTCGGCGGGGTTATTGCCTATGAAACCGTGGATGCAGCGGACATGCTGCAACCGGGCCAAAACAGTGGTTATCGGGTTTACAGCTCTGCCGCAACGGGTGATCACAGCTTCGGTCTGGGAGCCAGCGCCTTTGGCCGCACCGATGATCTGGATGGTATCCTCTCTTTCGGTACCCGTGATATCGGCAACATTCGTCAAAGCAACGGTTTTAATGCCCCGAATGATGAAACTATCAGTAATGTATTGGCAAAAGGCACTTGGCAGATAGACTCGATTCAATCGTTAAGCGCCAATCTGCGTTATTACAACAACAGTGCAATAGAGCCAAAGAATCCGCAAACCAGCGCACCATCCAGTACCAATGTCATGACCAATCGTTCAACGATCCAGCGTGATGCCCAGCTCAGATATAATATTAAGCCTCTCGATCAAGAATGGTTGAATGCCACGGCACAGGTTTATTACTCCGAAGTGGAAATCAATGCCCGTCCACAAGGTTCAGCCGAAGAAGGCCGCGAGCAGACAACCGAAGGTGTGAAGCTGGAAAACCGTACCCGTCTGTTTATCGAAAGCCCAGCCTCCCACTTGCTGACCTATGGTACTGAAACCTATAAACAGGAACAGACCCCCGGTGGGGCAACGGAAAGTTTCCCACAGGCCAAAATTCGGTTTAGTTCGGGCTGGCTACAAGATGAAATCACCTTACGCGACCTGCCTGTCTCCATATTAGCCGGTACGCGTTATGACAATTACAGTGGTAGCAGCGATGGCTATGCCGATGTCGATGCCGATAAATGGTCATCACGTGGTGCAATCAGCATTACACCAACTGATTGGCTGATGCTGTTTGGCTCCTATGCTCAGGCTTTCCGCGCCCCAACCATGGGTGAGATGTATAACGATTCCAAACACTTTGCGATCCCTATCCGGCCTGGGTTGACACTCACCAACTATTGGGTCCCCAACCCAAATCTGAAACCAGAAACCAATGAGACCCAGGAATACGGTTTTGGCCTACGTTTCAGTGATTTACTGATGGCTGAAGATGACCTGCAATTTAAAGTCAGTTACTTCGATACCAAGGCTAAAGACTACATCAGTACCCGCGTTGACATGCAAGCCATGACCACCACATCGGTGAATATCGATCAGGCAAAAATCTGGGGTTGGGATGCCAGCATGAGCTATAAAACGGCCTTGTTTAACTGGGATCTGGCCTATAACCGCACCCGTGGTAAAAATCAGAATACCGATGAATGGCTCGACACCATCAACCCGGATACCGTCACCAGTATCGTTGATGTACCTGTCGCCAACAGTGGTTTCTCCGTCGGTTGGATTGGGACCTTCGCTAATCGCTCCAGCCGCGTTTCCAGTTCAACACCGCAAGCCGGTTATGGCGTCAATGATTTCTATGTGAGCTATAAAGGCCAGGAAGCATTCAAAGGCATGACCACAACAATGTTATTGGGTAATGTCTTCGAAAAAGAGTATTACACGCCACAAGGCATCCCACAAGATGGCCGTAACGTGAAATTTTTCGTGAGTTATCAGTGG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,517 total letters Query= IDP00563.103 Length=2349 Score E Sequences producing significant alignments: (Bits) Value wt 3507 0.0 04E_E10_IDP00563.103_v73F.seq 62.6 1e-12 08E_E10_IDP00563.103_T7term.seq 39.2 1e-05 W S H P Q F E K G T E N L Y F Q S N A A D T T T T Q T S S Query_1 145 TGGTCTCATCCG-CAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGCTGATACCACAACCACTCAAACCAGCAGTAA 233 2 1 ................................ 32 0 47 ...........CA........G....------.......................... 98 K K H S T D T M V V T A T G N E R S S F E A P M M V T V I E Query_1 234 AAAGCACAGCACCGACACCATGGTCGTCACAGCAACCGGTAATGAGCGTAGCAGTTTTGAAGCGCCAATGATGGTAACGGTCATCGAAGG 323 2 33 .......................................................................................... 122 G N A P T S Q T A A T A A D M L R Q V P G L T V T G S G R T Query_1 324 GAATGCCCCTACCAGCCAAACCGCCGCGACGGCCGCAGATATGCTGCGACAAGTACCAGGGCTGACCGTCACTGGCAGCGGGCGTACCAA 413 2 123 .......................................................................................... 212 N G Q D V V M R G Y G K Q G V L T L V D G V R Q G T D T G H Query_1 414 CGGTCAAGATGTGGTGATGCGAGGTTACGGTAAACAAGGCGTGTTGACACTGGTTGATGGTGTTCGTCAGGGGACCGATACCGGTCACTT 503 2 213 .......................................................................................... 302 2 1663 ............ 1652 L N S T F L D P A L V K R I E I V R G P A A L L Y G S G A L Query_1 504 GAACTCCACTTTCCTCGATCCTGCGTTAGTCAAACGTATCGAAATCGTCCGTGGGCCAGCAGCACTGCTGTACGGCAGTGGCGCTCTCGG 593 2 303 .......................................................................................... 392 G G V I A Y E T V D A A D M L Q P G Q N S G Y R V Y S S A A Query_1 594 CGGGGTTATTGCCTATGAAACCGTGGATGCAGCGGACATGCTGCAACCGGGCCAAAACAGTGGTTATCGGGTTTACAGCTCTGCCGCAAC 683 2 393 .......................................................................................... 482 T G D H S F G L G A S A F G R T D D L D G I L S F G T R D I Query_1 684 GGGTGATCACAGCTTCGGTCTGGGAGCCAGCGCCTTTGGCCGCACCGATGATCTGGATGGTATCCTCTCTTTCGGTACCCGTGATATCGG 773 2 483 .......................................................................................... 572 G N I R Q S N G F N A P N D E T I S N V L A K G T W Q I D S Query_1 774 CAACATTCGTCAAAGCAACGGTTTTAATGCCCCGAATGATGAAACTATCAGTAATGTATTGGCAAAAGGCACTTGGCAGATAGACTCGAT 863 2 573 .......................................................................................... 662 I Q S L S A N L R Y Y N N S A I E P K N P Q T S A P S S T N Query_1 864 TCAATCGTTAAGCGCCAATCTGCGTTATTACAACAACAGTGCAATAGAGCCAAAGAATCCGCAAACCAGCGCACCATCCAGTACCAATGT 953 2 663 .......................................................................................... 752 V M T N R S T I Q R D A Q L R Y N I K P L D Q E W L N A T A Query_1 954 CATGACCAATCGTTCAACGATCCAGCGTGATGCCCAGCTCAGATATAATATTAAGCCTCTCGATCAAGAATGGTTGAATGCCACGGCACA 1043 2 753 .......................................................................................... 842 Q V Y Y S E V E I N A R P Q G S A E E G R E Q T T E G V K L Query_1 1044 GGTTTATTACTCCGAAGTGGAAATCAATGCCCGTCCACAAGGTTCAGCCGAAGAAGGCCGCGAGCAGACAACCGAAGGTGTGAAGCTGGA 1133 2 843 .......................................................................................... 932 E N R T R L F I E S P A S H L L T Y G T E T Y K Q E Q T P G Query_1 1134 AAACCGTACCCGTCTGTTTATCGAAAGCCCAGCCTCCCACTTGCTGACCTATGGTACTGAAACCTATAAACAGGAACAGACCCCCGGTGG 1223 2 933 .......................................................................................... 1022 G A T E S F P Q A K I R F S S G W L Q D E I T L R D L P V S Query_1 1224 GGCAACGGAAAGTTTCCCACAGGCCAAAATTCGGTTTAGTTCGGGCTGGCTACAAGATGAAATCACCTTACGCGACCTGCCTGTCTCCAT 1313 2 1023 .......................................................................................... 1112 I L A G T R Y D N Y S G S S D G Y A D V D A D K W S S R G A Query_1 1314 ATTAGCCGGTACGCGTTATGACAATTACAGTGGTAGCAGCGATGGCTATGCCGATGTCGATGCCGATAAATGGTCATCACGTGGTGCAAT 1403 2 1113 .......................................................................................... 1202 I S I T P T D W L M L F G S Y A Q A F R A P T M G E M Y N D Query_1 1404 CAGCATTACACCAACTGATTGGCTGATGCTGTTTGGCTCCTATGCTCAGGCTTTCCGCGCCCCAACCATGGGTGAGATGTATAACGATTC 1493 2 1203 .......................................................................................... 1292 S K H F A I P I R P G L T L T N Y W V P N P N L K P E T N E Query_1 1494 CAAACACTTTGCGATCCCTATCCGGCCTGGGTTGACACTCACCAACTATTGGGTCCCCAACCCAAATCTGAAACCAGAAACCAATGAGAC 1583 2 1293 .......................................................................................... 1382 T Q E Y G F G L R F S D L L M A E D D L Q F K V S Y F D T K Query_1 1584 CCAGGAATACGGTTTTGGCCTACGTTTCAGTGATTTACTGATGGCTGAAGATGACCTGCAATTTAAAGTCAGTTACTTCGATACCAAGGC 1673 2 1383 .......................................................................................... 1472 A K D Y I S T R V D M Q A M T T T S V N I D Q A K I W G W D Query_1 1674 TAAAGACTACATCAGTACCCGCGTTGACATGCAAGCCATGACCACCACATCGGTGAATATCGATCAGGCAAAAATCTGGGGTTGGGATGC 1763 2 1473 .......................................................................................... 1562 A S M S Y K T A L F N W D L A Y N R T R G K N Q N T D E W L Query_1 1764 CAGCATGAGCTATAAAACGGCCTTGTTTAACTGGGATCTGGCCTATAACCGCACCCGTGGTAAAAATCAGAATACCGATGAATGGCTCGA 1853 2 1563 .......................................................................................... 1652 2 275 . 275 2 1805 ........... 1815 D T I N P D T V T S I V D V P V A N S G F S V G W I G T F A Query_1 1854 CACCATCAACCCGGATACCGTCACCAGTATCGTTGATGTACCTGTCGCCAACAGTGGTTTCTCCGTCGGTTGGATTGGGACCTTCGCTAA 1943 2 1653 .......................................................................................... 1742 2 274 ........... 264 N R S S R V S S S T P Q A G Y G V N D F Y V S Y K G Q E A F Query_1 1944 TCGCTCCAGCCGCGTTTCCAGTTCAACACCGCAAGCCGGTTATGGCGTCAATGATTTCTATGTGAGCTATAAAGGCCAGGAAGCATTCAA 2033 2 1743 .......................................................................................... 1832 2 1568 ........... 1578 K G M T T T M L L G N V F E K E Y Y T P Q G I P Q D G R N V Query_1 2034 AGGCATGACCACAACAATGTTATTGGGTAATGTCTTCGAAAAAGAGTATTACACGCCACAAGGCATCCCACAAGATGGCCGTAACGTGAA 2123 2 1833 .......................................................................................... 1922 K F F V S Y Q W * H W K W I T D P N S S S V D K L A A A L E Query_1 2124 ATTTTTCGTGAGTTATCAGTGGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGca 2213 2 1923 ...................... 1944 1 66 ................... 48 H H H H H H * D P A A N K A R Query_1 2214 ccaccaccaccaccacTGAGATCCGGCTGCTAACAAAG-CCCGAAA 2258 1 45 ..................C....... 20 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 8082712 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:09 PM Number of letters in database: 3,517 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP05031.103 (IDP05031.22-528.P(528)..pMCSG73)
SGEKDSKQAGTSKSGIPKDGGTLTIGVSDNPETMNPLYANDRVSLTVQQALYAPLYHMEDGKKKFVLAESFTPSEDQLTWTLKLKDNLKWHDGKKITSDDIAFTFQSILDEKQNSSSRENFVFKGKPLEVKKVDELTTQFVLPQVSASFEGVMNDFFPIPKHVFEGEADLAKSKKNLQPVGSGPFKFKEYKSDEYVALDRFDDYVGGKAKLDSIVYRVVKDRNTANVSLQNGQINMKMIEPQDFKKLDSTGNFSMVTFPEGRLFYLSYNMNTDLMKKKEVRQAIAHALDKKEMINSAFVSSQFAEPANSILTPDAMYYAKDIKEYKYDKKEAKDLLAKAGVKDKEKVRVMYVTNNKIMESLALYTQQKLQEVGLEVELNALDASAASDKSLDKENKEYDITFGGYIMGPEPDSYKSLFLSNAEYNYARYKNADFDKLWEEAAVETDKTKRAELYHKIQETAREDLPYLPIAYPKAVIAVDKKFDGLKEAKAIPVTMFEDVSKIYEVK
TCAGGGGAAAAAGATTCGAAGCAAGCAGGAACTAGTAAATCAGGGATTCCGAAAGATGGAGGAACGTTAACGATTGGTGTTAGCGATAACCCAGAGACGATGAATCCGCTTTATGCGAATGATCGTGTTTCGTTAACGGTGCAGCAAGCTTTATATGCACCGCTGTATCATATGGAAGACGGTAAGAAGAAGTTTGTTCTTGCTGAGAGCTTTACGCCTTCAGAGGATCAATTAACATGGACTTTAAAACTAAAGGATAATTTGAAATGGCATGACGGTAAGAAGATTACATCAGACGACATAGCATTTACATTCCAATCTATTTTAGATGAGAAGCAAAATAGCTCAAGTCGTGAAAACTTTGTTTTTAAAGGAAAACCGCTTGAGGTGAAAAAGGTAGATGAGTTAACAACTCAATTCGTTTTACCACAAGTAAGTGCATCTTTCGAAGGTGTGATGAATGATTTCTTCCCAATTCCGAAACATGTATTTGAAGGGGAAGCAGATTTAGCGAAGAGTAAGAAAAACTTACAGCCTGTAGGATCAGGTCCGTTTAAGTTTAAAGAGTATAAGTCTGATGAGTACGTTGCATTAGATCGATTTGATGATTATGTAGGTGGTAAAGCTAAATTAGATTCTATCGTATACCGCGTTGTAAAAGATCGTAATACAGCAAATGTTTCACTACAAAATGGTCAAATCAACATGAAGATGATTGAGCCACAAGACTTTAAGAAATTAGATAGTACAGGGAACTTCTCAATGGTGACATTCCCAGAAGGTAGATTATTCTACTTATCTTATAACATGAATACAGATCTGATGAAGAAAAAAGAAGTGCGCCAAGCAATTGCACATGCGTTAGATAAAAAAGAAATGATTAACTCAGCATTCGTTTCTAGTCAATTTGCAGAACCAGCAAATTCAATTTTAACGCCAGACGCTATGTATTATGCAAAAGACATTAAAGAGTATAAGTATGATAAAAAAGAAGCGAAAGATTTATTAGCGAAAGCTGGCGTGAAGGATAAAGAAAAAGTACGCGTTATGTATGTAACGAATAATAAAATTATGGAAAGCTTAGCGCTTTATACACAACAAAAATTACAAGAAGTTGGCTTAGAAGTTGAACTGAATGCATTAGATGCTAGTGCAGCAAGTGACAAAAGCTTAGATAAAGAGAATAAAGAATATGACATTACATTCGGTGGTTACATAATGGGACCTGAGCCAGATTCATATAAGAGCTTATTCTTAAGTAATGCTGAATACAACTATGCACGTTATAAAAACGCTGATTTCGATAAGTTATGGGAAGAAGCTGCAGTTGAAACAGATAAAACAAAACGCGCAGAACTATATCATAAAATTCAAGAGACAGCTAGAGAAGACTTACCTTATCTACCAATTGCGTATCCGAAAGCAGTTATTGCAGTAGATAAAAAGTTTGATGGATTGAAAGAAGCAAAAGCAATTCCTGTCACAATGTTTGAAGATGTATCTAAGATTTATGAAGTGAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 1,531 total letters Query= IDP05031.103 Length=1926 Score E Sequences producing significant alignments: (Bits) Value wt 2744 0.0 S G E K D S K Q A G T S K S G I P K D G G T L T I G V S D N Query_1 202 TCAGGGGAAAAAGATTCGAAGCAAGCAGGAACTAGTAAATCAGGGATTCCGAAAGATGGAGGAACGTTAACGATTGGTGTTAGCGATAAC 291 2 1 .......................................................................................... 90 P E T M N P L Y A N D R V S L T V Q Q A L Y A P L Y H M E D Query_1 292 CCAGAGACGATGAATCCGCTTTATGCGAATGATCGTGTTTCGTTAACGGTGCAGCAAGCTTTATATGCACCGCTGTATCATATGGAAGAC 381 2 91 .......................................................................................... 180 2 274 ... 276 G K K K F V L A E S F T P S E D Q L T W T L K L K D N L K W Query_1 382 GGTAAGAAGAAGTTTGTTCTTGCTGAGAGCTTTACGCCTTCAGAGGATCAATTAACATGGACTTTAAAACTAAAGGATAATTTGAAATGG 471 2 181 .......................................................................................... 270 2 277 .......... 286 H D G K K I T S D D I A F T F Q S I L D E K Q N S S S R E N Query_1 472 CATGACGGTAAGAAGATTACATCAGACGACATAGCATTTACATTCCAATCTATTTTAGATGAGAAGCAAAATAGCTCAAGTCGTGAAAAC 561 2 271 .......................................................................................... 360 2 178 ............. 190 F V F K G K P L E V K K V D E L T T Q F V L P Q V S A S F E Query_1 562 TTTGTTTTTAAAGGAAAACCGCTTGAGGTGAAAAAGGTAGATGAGTTAACAACTCAATTCGTTTTACCACAAGTAAGTGCATCTTTCGAA 651 2 361 .......................................................................................... 450 G V M N D F F P I P K H V F E G E A D L A K S K K N L Q P V Query_1 652 GGTGTGATGAATGATTTCTTCCCAATTCCGAAACATGTATTTGAAGGGGAAGCAGATTTAGCGAAGAGTAAGAAAAACTTACAGCCTGTA 741 2 451 .......................................................................................... 540 G S G P F K F K E Y K S D E Y V A L D R F D D Y V G G K A K Query_1 742 GGATCAGGTCCGTTTAAGTTTAAAGAGTATAAGTCTGATGAGTACGTTGCATTAGATCGATTTGATGATTATGTAGGTGGTAAAGCTAAA 831 2 541 .......................................................................................... 630 2 965 ...............A.... 984 L D S I V Y R V V K D R N T A N V S L Q N G Q I N M K M I E Query_1 832 TTAGATTCTATCGTATACCGCGTTGTAAAAGATCGTAATACAGCAAATGTTTCACTACAAAATGGTCAAATCAACATGAAGATGATTGAG 921 2 631 .......................................................................................... 720 P Q D F K K L D S T G N F S M V T F P E G R L F Y L S Y N M Query_1 922 CCACAAGACTTTAAGAAATTAGATAGTACAGGGAACTTCTCAATGGTGACATTCCCAGAAGGTAGATTATTCTACTTATCTTATAACATG 1011 2 721 .......................................................................................... 810 N T D L M K K K E V R Q A I A H A L D K K E M I N S A F V S Query_1 1012 AATACAGATCTGATGAAGAAAAAAGAAGTGCGCCAAGCAATTGCACATGCGTTAGATAAAAAAGAAATGATTAACTCAGCATTCGTTTCT 1101 2 811 .......................................................................................... 900 2 982 ............ 993 S Q F A E P A N S I L T P D A M Y Y A K D I K E Y K Y D K K Query_1 1102 AGTCAATTTGCAGAACCAGCAAATTCAATTTTAACGCCAGACGCTATGTATTATGCAAAAGACATTAAAGAGTATAAGTATGATAAAAAA 1191 2 901 .......................................................................................... 990 2 560 ...............C.... 579 2 865 ......... 873 E A K D L L A K A G V K D K E K V R V M Y V T N N K I M E S Query_1 1192 GAAGCGAAAGATTTATTAGCGAAAGCTGGCGTGAAGGATAAAGAAAAAGTACGCGTTATGTATGTAACGAATAATAAAATTATGGAAAGC 1281 2 991 .......................................................................................... 1080 2 874 ... 876 L A L Y T Q Q K L Q E V G L E V E L N A L D A S A A S D K S Query_1 1282 TTAGCGCTTTATACACAACAAAAATTACAAGAAGTTGGCTTAGAAGTTGAACTGAATGCATTAGATGCTAGTGCAGCAAGTGACAAAAGC 1371 2 1081 .......................................................................................... 1170 L D K E N K E Y D I T F G G Y I M G P E P D S Y K S L F L S Query_1 1372 TTAGATAAAGAGAATAAAGAATATGACATTACATTCGGTGGTTACATAATGGGACCTGAGCCAGATTCATATAAGAGCTTATTCTTAAGT 1461 2 1171 .......................................................................................... 1260 N A E Y N Y A R Y K N A D F D K L W E E A A V E T D K T K R Query_1 1462 AATGCTGAATACAACTATGCACGTTATAAAAACGCTGATTTCGATAAGTTATGGGAAGAAGCTGCAGTTGAAACAGATAAAACAAAACGC 1551 2 1261 .......................................................................................... 1350 A E L Y H K I Q E T A R E D L P Y L P I A Y P K A V I A V D Query_1 1552 GCAGAACTATATCATAAAATTCAAGAGACAGCTAGAGAAGACTTACCTTATCTACCAATTGCGTATCCGAAAGCAGTTATTGCAGTAGAT 1641 2 1351 .......................................................................................... 1440 K K F D G L K E A K A I P V T M F E D V S K I Y E V K Query_1 1642 AAAAAGTTTGATGGATTGAAAGAAGCAAAAGCAATTCCTGTCACAATGTTTGAAGATGTATCTAAGATTTATGAAGTGAAA 1722 2 1441 ................................................................................. 1521 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2839746 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:09 PM Number of letters in database: 1,531 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP05157.103 (IDP05157.23-543.P(543)..pMCSG73)
YQKEDNKAGAKEKSSNKQVLNLTETAEIPTMDTTLSTDATSSNIMNNTMEGLYRLGKEDKLVPGVAKSYEKSEDGKKYVFKLREDAKWSNGEPVTAKDFVYSWRRAVDSNTGAKFAYILFDVKNAEKVNKKELPVEELGVKAIDDHTLEVELDNPVPYFVSLTVYPTLYPLNEKFVTEQGAKFGLESNTTLYNGPFVLNEWKHEQSFQLKKNPSYWENKEVKLEEINFNIVKDRSTAINLYETKAIDRVVLTSEFVDKYKSDADFKTIKKPSTQFIRLNEKNKFLANKNIRKAIAMSFERENIGKVILNDGSEGIYGFVPKGLAKGPNGKDFREENGKLIKEDMKEAQKYWEAGKKELGVDKVELELLNFDTDDAKKIGEYLKGQFEKNLPGLTVPTKMQPFAQKLKLEASGDYAMSYAGWSPDYMDPMSFLEMYTTGNAQNKVHYANPAYDDLIKKAKTEVDVQARWDALLQAEKQLLEDAAIAPVYQPGKAYLQRGSITGLLEHKYGGEFSYKWVELKN
TACCAAAAAGAGGATAATAAAGCAGGCGCAAAAGAAAAATCCTCAAACAAGCAAGTGCTAAATCTAACTGAAACAGCTGAAATTCCAACTATGGATACGACGTTATCAACAGATGCAACATCTTCTAACATCATGAATAACACAATGGAAGGGTTATATCGTCTGGGGAAAGAGGATAAACTTGTTCCAGGTGTCGCTAAATCTTATGAGAAATCAGAGGATGGCAAAAAGTATGTGTTTAAGTTACGTGAAGATGCGAAATGGTCTAATGGTGAACCGGTAACAGCGAAAGACTTCGTTTATTCTTGGCGAAGAGCTGTAGATTCAAACACGGGTGCCAAGTTTGCTTACATACTATTTGATGTTAAAAATGCGGAGAAAGTTAACAAAAAAGAATTACCAGTTGAAGAACTTGGTGTAAAGGCAATTGATGATCATACACTTGAAGTGGAATTAGATAACCCTGTTCCTTATTTTGTAAGTTTAACAGTCTATCCAACATTGTATCCTTTGAATGAGAAGTTTGTAACAGAACAAGGAGCAAAATTTGGATTAGAATCCAATACGACACTTTATAATGGTCCATTCGTATTAAATGAATGGAAGCATGAACAAAGTTTCCAACTTAAGAAGAATCCATCGTATTGGGAAAATAAAGAAGTAAAACTTGAGGAAATAAACTTCAATATTGTTAAGGATCGTTCAACTGCTATAAATTTATATGAAACAAAAGCAATTGATCGTGTAGTCTTAACATCAGAGTTTGTAGATAAATACAAATCAGATGCTGACTTTAAAACGATTAAGAAACCATCTACACAATTTATTCGCTTAAATGAAAAGAATAAATTTTTAGCAAATAAAAATATCCGAAAAGCAATTGCAATGTCCTTTGAACGTGAAAACATTGGAAAAGTGATCTTAAACGATGGTTCAGAGGGTATATACGGTTTTGTTCCGAAAGGCTTAGCAAAAGGTCCGAATGGAAAAGACTTCCGTGAAGAAAACGGAAAGCTTATAAAAGAGGACATGAAAGAAGCACAAAAATACTGGGAAGCTGGTAAAAAAGAACTTGGTGTAGACAAAGTAGAGTTAGAGCTATTAAACTTTGATACTGATGATGCTAAAAAAATTGGAGAGTATTTAAAAGGCCAATTTGAAAAGAATTTACCAGGTTTAACAGTTCCAACAAAAATGCAGCCATTTGCACAAAAATTAAAACTGGAAGCAAGTGGAGATTATGCAATGTCATATGCGGGATGGAGTCCAGATTATATGGATCCAATGTCATTCCTTGAAATGTATACGACAGGTAATGCGCAAAACAAAGTACATTACGCAAACCCAGCGTATGATGATTTAATTAAAAAAGCAAAAACAGAAGTTGATGTACAAGCTCGCTGGGATGCATTATTACAAGCGGAAAAACAACTGCTTGAAGATGCAGCGATTGCTCCGGTATATCAACCTGGAAAAGCATATTTACAACGTGGCTCAATTACTGGCCTATTAGAACATAAATATGGCGGAGAATTTAGCTATAAGTGGGTTGAACTTAAAAAC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,393 total letters Query= IDP05157.103 Length=1968 Score E Sequences producing significant alignments: (Bits) Value wt 2819 0.0 02E_E8_IDP05157.103_v73F.seq 1590 0.0 06E_E8_IDP05157.103_T7term.seq 1364 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 0 41 ...........A........G....------......................N 88 0 23 .....C........... 39 A Y Q K E D N K A G A K E K S S N K Q V L N L T E T A E I P Query_1 199 GCCTACCAAAAAGAGGATAATAAAGCAGGCGCAAAAGAAAAATCCTCAAACAAGCAAGTGCTAAATCTAACTGAAACAGCTGAAATTCCA 288 2 1 ....................................................................................... 87 2 169 ........... 179 0 89 .......................................................................................... 178 0 260 ........... 270 T M D T T L S T D A T S S N I M N N T M E G L Y R L G K E D Query_1 289 ACTATGGATACGACGTTATCAACAGATGCAACATCTTCTAACATCATGAATAACACAATGGAAGGGTTATATCGTCTGGGGAAAGAGGAT 378 2 88 .......................................................................................... 177 2 7 ......... 15 0 179 .......................................................................................... 268 0 98 ......... 106 K L V P G V A K S Y E K S E D G K K Y V F K L R E D A K W S Query_1 379 AAACTTGTTCCAGGTGTCGCTAAATCTTATGAGAAATCAGAGGATGGCAAAAAGTATGTGTTTAAGTTACGTGAAGATGCGAAATGGTCT 468 2 178 .......................................................................................... 267 2 16 .. 17 0 269 ................................................................................N......... 358 0 107 .. 108 N G E P V T A K D F V Y S W R R A V D S N T G A K F A Y I L Query_1 469 AATGGTGAACCGGTAACAGCGAAAGACTTCGTTTATTCTTGGCGAAGAGCTGTAGATTCAAACACGGGTGCCAAGTTTGCTTACATACTA 558 2 268 .......................................................................................... 357 0 359 .......................................................................................... 448 F D V K N A E K V N K K E L P V E E L G V K A I D D H T L E Query_1 559 TTTGATGTTAAAAATGCGGAGAAAGTTAACAAAAAAGAATTACCAGTTGAAGAACTTGGTGTAAAGGCAATTGATGATCATACACTTGAA 648 2 358 .......................................................................................... 447 2 1067 .............. 1080 0 449 .......................................................................................... 538 1 565 .............. 552 V E L D N P V P Y F V S L T V Y P T L Y P L N E K F V T E Q Query_1 649 GTGGAATTAGATAACCCTGTTCCTTATTTTGTAAGTTTAACAGTCTATCCAACATTGTATCCTTTGAATGAGAAGTTTGTAACAGAACAA 738 2 448 .......................................................................................... 537 0 539 .......................................................................................... 628 G A K F G L E S N T T L Y N G P F V L N E W K H E Q S F Q L Query_1 739 GGAGCAAAATTTGGATTAGAATCCAATACGACACTTTATAATGGTCCATTCGTATTAAATGAATGGAAGCATGAACAAAGTTTCCAACTT 828 2 538 .......................................................................................... 627 0 629 .......................................................................................... 718 K K N P S Y W E N K E V K L E E I N F N I V K D R S T A I N Query_1 829 AAGAAGAATCCATCGTATTGGGAAAATAAAGAAGTAAAACTTGAGGAAATAAACTTCAATATTGTTAAGGATCGTTCAACTGCTATAAAT 918 2 628 .......................................................................................... 717 2 722 ....... 716 0 719 .......................................................................................... 808 0 813 ....... 807 L Y E T K A I D R V V L T S E F V D K Y K S D A D F K T I K Query_1 919 TTATATGAAACAAAAGCAATTGATCGTGTAGTCTTAACATCAGAGTTTGTAGATAAATACAAATCAGATGCTGACTTTAAAACGATTAAG 1008 2 718 .......................................................................................... 807 2 715 ..... 711 2 873 ........... 883 0 809 ...................................-.......................................-.........-.... 895 0 806 ..... 802 1 844 ................... 826 1 759 ........... 749 K P S T Q F I R L N E K N K F L A N K N I R K A I A M S F Query_1 1009 AAACCATCTACACAATTT-ATTCGCTTAAATGAAAAGAATAAATTTTTAGCAAATAAAAATATCCGAAAA-GCAATTGCAATGTCCTTTG 1096 2 808 ..................-...................................................-................... 895 2 729 ....-....... 739 0 896 ...........N.-....-......-......-.......-..............--.........-...-..-.....-........C. 974 0 820 ....-....... 830 1 825 ..-...............T...................NN...A....................N.....A................... 737 E R E N I G K V I L N D G S E G I Y G F V P K G L A K G P N Query_1 1097 AACGTGAAAACATTGGAAAAGTGATCTTAAACGATGGTTCAGA-GGGTATATACGGTTTTGTTCCGAAAGGCTTAGCAAAAGGTCCGAAT 1185 2 896 ...........................................-.............................................. 984 0 975 ......... 983 1 736 ..............................-............A.............................................. 648 G K D F R E E N G K L I K E D M K E A Q K Y W E A G K K E L Query_1 1186 GGAAAAGACTTCCGTGAAGAAAACGGAAAGCTTATAAAAGAGGACATGAAAGAAGCACAAAAATACTGGGAAGCTGGTAAAAAAGAACTT 1275 2 985 .......................................................................................... 1074 2 407 ........ 414 0 498 ........ 505 1 647 ................................................................G......................... 558 G V D K V E L E L L N F D T D D A K K I G E Y L K G Q F E K Query_1 1276 GGTGTAGACAAAGTAGAGTTAGAGCTATTAAACTTTGATACTGATGATGCTaaaaaaaTTGGAGAGTATTTAAAAGGCCAATTTGAAAAG 1365 2 1075 .......................................................................................... 1164 2 415 ...... 420 0 506 ...... 511 1 557 ........................................N................................................. 468 N L P G L T V P T K M Q P F A Q K L K L E A S G D Y A M S Y Query_1 1366 AATTTACCAGGTTTAACAGTTCCAACAAAAATGCAGCCATTTGCACAAAAATTAAAACTGGAAGCAAGTGGAGATTATGCAATGTCATAT 1455 2 1165 .......................................................................................... 1254 1 467 ...................................N....N................................................. 378 A G W S P D Y M D P M S F L E M Y T T G N A Q N K V H Y A N Query_1 1456 GCGGGATGGAGTCCAGATTATATGGATCCAATGTCATTCCTTGAAATGTATACGACAGGTAATGCGCAAAACAAAGTACATTACGCAAAC 1545 2 1255 .......................................................................................... 1344 1 377 .......................................................................................... 288 P A Y D D L I K K A K T E V D V Q A R W D A L L Q A E K Q L Query_1 1546 CCAGCGTATGATGATTTAATTaaaaaagcaaaaaCAGAAGTTGATGTACAAGCTCGCTGGGATGCATTATTACAAGCGGAAAAACAACTG 1635 2 1345 .......................................................................................... 1434 1 287 .......................................................................................... 198 L E D A A I A P V Y Q P G K A Y L Q R G S I T G L L E H K Y Query_1 1636 CTTGAAGATGCAGCGATTGCTCCGGTATATCAACCTGGAAAAGCATATTTACAACGTGGCTCAATTACTGGCCTATTAGAACATAAATAT 1725 2 1435 .......................................................................................... 1524 1 197 .......................................................................................... 108 G G E F S Y K W V E L K N * H W K W I T D P N S S S V D K L Query_1 1726 GGCGGAGAATTTAGCTATAAGTGGGTTGAACTTAAAAACTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTT 1815 2 1525 ....................................... 1563 1 107 ................T..........................................--..... 44 A A A L E H H H H H H * D P A A N K A R K E Query_1 1816 GCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAAC-AAAGCCC--GAAAGGAA 1881 1 48 ..............A.......CN....N... 17 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 6529440 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 3,393 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP05160.103 (IDP05160.25-256.P(256)..pMCSG73)
KAHTNKVAIVIDDFGNNMKGTDKMLSLPIPLTVAVMPFLPSTKEDAIAAHKKGHEVIIHMPMEPIKGKKEWLGPKAITTDLSDEEINNRLEQAIQEVPHAIGMNNHMGSKVTADERIVRLILAACKKHGLFYLDSKTNPKSVVPKIGKELGVPIIENQLFFDDVYTAAHISKQAQLLIKKLQEKPIMVAIGHVGPPGEITSRVIETSIPKIREHADFIFLSDLALSPPPVSK
AAGGCCCATACAAACAAAGTAGCTATTGTCATTGATGACTTCGGCAATAATATGAAGGGGACTGATAAAATGTTATCACTTCCTATTCCCCTAACTGTTGCTGTTATGCCTTTTCTTCCTTCCACAAAGGAAGATGCGATAGCGGCTCATAAGAAAGGACACGAAGTTATTATACATATGCCAATGGAACCCATTAAAGGTAAAAAAGAGTGGCTTGGACCAAAAGCAATTACAACTGATTTAAGCGACGAAGAAATAAACAATCGACTCGAACAAGCAATTCAAGAAGTACCACATGCAATTGGGATGAATAATCATATGGGATCAAAAGTGACTGCAGACGAAAGAATTGTACGACTTATACTTGCAGCTTGTAAAAAACACGGCTTATTTTATTTAGATAGCAAAACAAACCCTAAAAGTGTCGTCCCAAAAATTGGGAAAGAATTAGGGGTGCCAATTATTGAAAATCAATTATTTTTTGATGATGTGTATACTGCGGCACATATTTCAAAACAAGCCCAATTACTCATAAAAAAACTTCAAGAAAAACCGATTATGGTGGCTATTGGACATGTTGGCCCTCCAGGTGAAATTACATCACGTGTTATAGAAACTTCTATTCCTAAAATTCGTGAACACGCAGATTTTATTTTTTTATCTGATTTAGCATTATCTCCACCTCCTGTTTCGAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 1,104 total letters Query= IDP05160.103 Length=1101 Score E Sequences producing significant alignments: (Bits) Value wt 1256 0.0 06A_A8_IDP05160.103_T7term.seq 627 0.0 I H M H H H H H H S S G V D L W S H P Q F E K G T E N L Y Query_1 99 TATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTT 188 2 171 ........... 181 F Q S N A K A H T N K V A I V I D D F G N N M K G T D K M L Query_1 189 CCAATCCAATGCCAAGGCCCATACAAACAAAGTAGCTATTGTCATTGATGACTTCGGCAATAATATGAAGGGGACTGATAAAATGTTATC 278 2 1 ............................................................................. 77 S L P I P L T V A V M P F L P S T K E D A I A A H K K G H E Query_1 279 ACTTCCTATTCCCCTAACTGTTGCTGTTATGCCTTTTCTTCCTTCCACAAAGGAAGATGCGATAGCGGCTCATAAGAAAGGACACGAAGT 368 2 78 .......................................................................................... 167 V I I H M P M E P I K G K K E W L G P K A I T T D L S D E E Query_1 369 TATTATACATATGCCAATGGAACCCATTAAAGGTAAAAAAGAGTGGCTTGGACCAAAAGCAATTACAACTGATTTAAGCGACGAAGAAAT 458 2 168 .......................................................................................... 257 I N N R L E Q A I Q E V P H A I G M N N H M G S K V T A D E Query_1 459 AAACAATCGACTCGAACAAGCAATTCAAGAAGTACCACATGCAATTGGGATGAATAATCATATGGGATCAAAAGTGACTGCAGACGAAAG 548 2 258 .......................................................................................... 347 R I V R L I L A A C K K H G L F Y L D S K T N P K S V V P K Query_1 549 AATTGTACGACTTATACTTGCAGCTTGTAAAAAACACGGCTTATTTTATTTAGATAGCAAAACAAACCCTAAAAGTGTCGTCCCAAAAAT 638 2 348 .......................................................................................... 437 1 403 ................................................................................. 323 I G K E L G V P I I E N Q L F F D D V Y T A A H I S K Q A Q Query_1 639 TGGGAAAGAATTAGGGGTGCCAATTATTGAAAATCAATTATTTTTTGATGATGTGTATACTGCGGCACATATTTCAAAACAAGCCCAATT 728 2 438 .......................................................................................... 527 1 322 .......................................................................................... 233 L L I K K L Q E K P I M V A I G H V G P P G E I T S R V I E Query_1 729 ACTCATaaaaaaaCTTCAAGAAAAACCGATTATGGTGGCTATTGGACATGTTGGCCCTCCAGGTGAAATTACATCACGTGTTATAGAAAC 818 2 528 .......................................................................................... 617 1 232 .......................................................................................... 143 T S I P K I R E H A D F I F L S D L A L S P P P V S K * H W Query_1 819 TTCTATTCCTAAAATTCGTGAACACGCAGATTTTAtttttttATCTGATTTAGCATTATCTCCACCTCCTGTTTCGAAATAACATTGGAA 908 2 618 ............................................................................... 696 1 142 ................N...................................N..A.-G............................... 54 K W I T D P N S S S V D K L A A A L E H H H H H H * D P A A Query_1 909 GTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAA 998 1 53 .........--..... 40 1 44 ............. 32 N K A R K E Query_1 999 CAAAGCCCGAAAGGAAG 1015 1 31 .......N....A.... 15 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1154394 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 1,104 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP05180.103 (IDP05180.24-568.P(568)..pMCSG73)
ACSGSGGEKKSTTTSSGGGEEKKSEIKYAAKQVLNRTENQEIPTMDVSKSTDTLGSQILGNTMEGLYRLDKDNKPIPAAAESSTKSEDGKKYTFKLRKDAKWSNGDPVTAKDFVYAWQRLLDKNTAAEYAFIAYYIKNAEAINKGEKPLTDLGAKAVDDYTLEVELEKPVPYFLNLMAFPSYYPLNEKFVKEKGDKFGLEADTTLYNGPFVMASWKHEQGWQLKKNDKYWDNKTVKLEEINYSVVKEVATKVNLYDTGSIDFTLLSGEFVDKYKSNKEEYGEYSEASTFFLRLNQKRNGQDTPLKSKKLREAIALSIDKKGLATVILNNGSKATDQLVPKGLATGPDGKDYQDTFKNGLKYDPKKGAAAWEEAKKELGKDQVTIELLSYDDGTAKKIADYFKDQIEKNLKGVTVNTKIQPFKQKLKLESAQDYEVSFAGWSPDYSDPMTFIDMFESKSPYNQMSYSNPKYDEMVAKAGNELLSDPKKRWETLGKAEKLFLEEDAGLVPLYQTGRAYVMKPNVKGIVKHNISPEYSFKWAYVTEGK
GCTTGTTCTGGATCTGGTGGGGAGAAGAAATCAACTACGACGTCTAGTGGTGGTGGGGAAGAGAAAAAGTCTGAAATTAAATACGCAGCGAAACAAGTGTTAAATCGTACAGAGAATCAAGAGATTCCGACGATGGATGTTTCAAAATCTACCGATACATTAGGTTCTCAAATTTTAGGGAACACGATGGAAGGTTTATATCGATTAGATAAAGATAATAAGCCAATCCCAGCTGCAGCAGAATCTAGTACGAAAAGCGAGGATGGCAAAAAATATACATTTAAATTACGTAAAGATGCAAAATGGTCAAATGGTGATCCTGTAACAGCGAAAGATTTCGTATATGCATGGCAGCGCTTACTTGATAAAAATACAGCGGCAGAATATGCATTTATTGCTTACTATATTAAAAACGCAGAGGCAATTAATAAAGGTGAAAAACCACTAACAGATTTAGGAGCAAAAGCGGTAGATGATTATACGCTAGAAGTAGAATTAGAGAAACCAGTACCATATTTCTTGAATTTAATGGCATTCCCATCTTACTATCCTTTAAATGAAAAGTTCGTAAAAGAAAAAGGAGATAAATTCGGTTTAGAAGCAGATACAACGTTGTATAACGGACCGTTCGTTATGGCTTCATGGAAACATGAACAAGGATGGCAGCTAAAGAAAAATGATAAGTACTGGGATAATAAGACTGTAAAATTAGAAGAAATTAACTATAGTGTAGTAAAAGAAGTTGCGACGAAAGTAAACTTATATGATACAGGATCAATTGATTTCACGTTATTATCAGGAGAATTCGTTGATAAATATAAATCGAACAAAGAAGAGTACGGCGAGTATTCGGAAGCAAGTACATTCTTCTTACGTTTAAATCAAAAGCGTAACGGACAAGATACACCGTTAAAGAGCAAAAAACTTCGTGAAGCGATCGCATTATCAATTGATAAAAAAGGATTAGCAACCGTTATTTTAAATAACGGTTCAAAAGCAACAGATCAATTAGTACCAAAAGGGCTTGCGACAGGACCAGACGGTAAAGACTACCAAGATACGTTTAAAAATGGTCTAAAATATGATCCGAAAAAAGGTGCAGCAGCTTGGGAAGAAGCGAAAAAAGAACTTGGAAAAGATCAAGTGACAATTGAATTACTAAGCTATGATGATGGAACTGCGAAAAAAATTGCTGACTACTTTAAAGATCAAATTGAGAAAAACTTAAAAGGTGTAACGGTTAACACGAAAATTCAACCGTTCAAACAAAAACTAAAATTAGAGTCAGCACAAGATTATGAAGTTTCGTTTGCAGGTTGGAGTCCAGATTATTCGGATCCAATGACATTTATTGATATGTTTGAATCGAAGAGCCCATATAACCAAATGAGTTATTCGAATCCAAAATATGATGAAATGGTAGCGAAAGCAGGTAATGAATTACTGTCTGATCCGAAGAAGCGTTGGGAAACGTTAGGAAAAGCAGAGAAATTATTCCTTGAAGAAGATGCAGGATTAGTTCCTTTATATCAAACAGGAAGAGCGTATGTAATGAAACCGAATGTAAAAGGAATTGTGAAACATAACATTAGTCCAGAATATAGCTTTAAGTGGGCTTATGTAACGGAAGGTAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,310 total letters Query= IDP05180.103 Length=2040 Score E Sequences producing significant alignments: (Bits) Value wt 2949 0.0 02C_C8_IDP05180.103_v73F.seq 693 0.0 06C_C8_IDP05180.103_T7term.seq 192 5e-52 W S H P Q F E K G T E N L Y F Q S N A A C S G S G G E K K S Query_1 145 TGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGCTTGTTCTGGATCTGGTGGGGAGAAGAAATCA 234 2 1 ................................. 33 0 36 ...........A.N......G....------....................TT.........N........................... 119 T T T S S G G G E E K K S E I K Y A A K Q V L N R T E N Q E Query_1 235 ACTACGACGTCTAGTGGTGGTGGGGAAGAGAAAAAGTCTGAAATTAAATACGCAGCGAAACAAGTGTTAAATCGTACAGAGAATCAAGAG 324 2 34 .......................................................................................... 123 0 120 ........................................................T................................. 209 I P T M D V S K S T D T L G S Q I L G N T M E G L Y R L D K Query_1 325 ATTCCGACGATGGATGTTTCAAAATCTACCGATACATTAGGTTCTCAAATTTTAGGGAACACGATGGAAGGTTTATATCGATTAGATAAA 414 2 124 .......................................................................................... 213 0 210 .......................................N..............N...........T.................T....N 299 D N K P I P A A A E S S T K S E D G K K Y T F K L R K D A K Query_1 415 GATAATAAGCCAATCCCAGCTGCAGCAGAATCTAGTACGAAAAGCGAGGATGGCAAAAAATATACATTTAAATTACGTAAAGATGCAAAA 504 2 214 .......................................................................................... 303 0 300 ........................T........NN..................NN..T..................T............. 389 W S N G D P V T A K D F V Y A W Q R L L D K N T A A E Y A F Query_1 505 TGGTCAAATGGTGATCCTGTAACAGCGAAAGATTTCGTATATGCATGGCAGCGCTTACTTGATAAAAATACAGCGGCAGAATATGCATTT 594 2 304 .......................................................................................... 393 0 390 .......................NN.N.............T.......N.......................N................. 479 I A Y Y I K N A E A I N K G E K P L T D L G A K A V D D Y T Query_1 595 ATTGCTTACTATATTAAAAACGCAGAGGCAATTAATAAAGGTGAAAAACCACTAACAGATTTAGGAGCAAAAGCGGTAGATGATTATACG 684 2 394 .......................................................................................... 483 0 480 .... 483 L E V E L E K P V P Y F L N L M A F P S Y Y P L N E K F V K Query_1 685 CTAGAAGTAGAATTAGAGAAACCAGTACCATATTTCTTGAATTTAATGGCATTCCCATCTTACTATCCTTTAAATGAAAAGTTCGTAAAA 774 2 484 .......................................................................................... 573 E K G D K F G L E A D T T L Y N G P F V M A S W K H E Q G W Query_1 775 GAAAAAGGAGATAAATTCGGTTTAGAAGCAGATACAACGTTGTATAACGGACCGTTCGTTATGGCTTCATGGAAACATGAACAAGGATGG 864 2 574 .......................................................................................... 663 2 618 .............. 605 Q L K K N D K Y W D N K T V K L E E I N Y S V V K E V A T K Query_1 865 CAGCTAAAGAAAAATGATAAGTACTGGGATAATAAGACTGTAAAATTAGAAGAAATTAACTATAGTGTAGTAAAAGAAGTTGCGACGAAA 954 2 664 .......................................................................................... 753 V N L Y D T G S I D F T L L S G E F V D K Y K S N K E E Y G Query_1 955 GTAAACTTATATGATACAGGATCAATTGATTTCACGTTATTATCAGGAGAATTCGTTGATAAATATAAATCGAACAAAGAAGAGTACGGC 1044 2 754 .......................................................................................... 843 E Y S E A S T F F L R L N Q K R N G Q D T P L K S K K L R E Query_1 1045 GAGTATTCGGAAGCAAGTACATTCTTCTTACGTTTAAATCAAAAGCGTAACGGACAAGATACACCGTTAAAGAGCAAAAAACTTCGTGAA 1134 2 844 .......................................................................................... 933 A I A L S I D K K G L A T V I L N N G S K A T D Q L V P K G Query_1 1135 GCGATCGCATTATCAATTGATAAAAAAGGATTAGCAACCGTTATTTTAAATAACGGTTCAAAAGCAACAGATCAATTAGTACCAAAAGGG 1224 2 934 .......................................................................................... 1023 2 991 ...........A........... 969 2 956 .............. 943 L A T G P D G K D Y Q D T F K N G L K Y D P K K G A A A W E Query_1 1225 CTTGCGACAGGACCAGACGGTAAAGACTACCAAGATACGTTTAAAAATGGTCTAAAATATGATCCGAAAAAAGGTGCAGCAGCTTGGGAA 1314 2 1024 .......................................................................................... 1113 E A K K E L G K D Q V T I E L L S Y D D G T A K K I A D Y F Query_1 1315 GAAGCGAAAAAAGAACTTGGAAAAGATCAAGTGACAATTGAATTACTAAGCTATGATGATGGAACTGCGaaaaaaaTTGCTGACTACTTT 1404 2 1114 .......................................................................................... 1203 K D Q I E K N L K G V T V N T K I Q P F K Q K L K L E S A Q Query_1 1405 AAAGATCAAATTGAGAAAAACTTAAAAGGTGTAACGGTTAACACGAAAATTCAACCGTTCAAACAAAAACTAAAATTAGAGTCAGCACAA 1494 2 1204 .......................................................................................... 1293 D Y E V S F A G W S P D Y S D P M T F I D M F E S K S P Y N Query_1 1495 GATTATGAAGTTTCGTTTGCAGGTTGGAGTCCAGATTATTCGGATCCAATGACATTTATTGATATGTTTGAATCGAAGAGCCCATATAAC 1584 2 1294 .......................................................................................... 1383 Q M S Y S N P K Y D E M V A K A G N E L L S D P K K R W E T Query_1 1585 CAAATGAGTTATTCGAATCCAAAATATGATGAAATGGTAGCGAAAGCAGGTAATGAATTACTGTCTGATCCGAAGAAGCGTTGGGAAACG 1674 2 1384 .......................................................................................... 1473 L G K A E K L F L E E D A G L V P L Y Q T G R A Y V M K P N Query_1 1675 TTAGGAAAAGCAGAGAAATTATTCCTTGAAGAAGATGCAGGATTAGTTCCTTTATATCAAACAGGAAGAGCGTATGTAATGAAACCGAAT 1764 2 1474 .......................................................................................... 1563 1 192 .............................................................-.......... 122 V K G I V K H N I S P E Y S F K W A Y V T E G K * H W K Query_1 1765 GTAAAAGGAATTGTGAAACATAACATTAGTCCAGAATATAGCTTTAAGTGGGCTTATGTAACGGAAGGTAAATAACATTGGAAG 1848 2 1564 ........................................................................ 1635 1 121 ..........-N.........-...-.................A.-.T.....--NA................-...-...... 46 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4578288 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 2,310 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP05319.103 (IDP05319.24-540.P(540)..pMCSG73)
KEKTTTKPKDEKKVLQLLETGEIPSLHSGKVTDAVSFNVLNNVMEGLFRLSKNDEVIEAGAQKYEVSKDGKTYTFHLRDAKWSNGDPVTAQDYVYAWKQLINPDTASQYAYIAYDVKNAEKINKKQLGLDELGVKAKDDKTFVVELEHPVPYFTKLLILPSFYPINEKYAKEQGDKYGLEANKAVYNGPFTLSDWKHEASFTMKKNDKYWDKKEVKLDEVNYQIVKEISTAVNLYETDKVDRAVISTEFVDKYKNNKELKQYTDPVMYFFRFNENVPILKNKNARLALSTVFDKKGLATSFLNDGSVAANYYVPKGFLKGPDKKDFRSTAGEFNKTDVKQAKEYWEKAKQETGTNEVTLELLNYDLENFKKVGEYIKEQLEKNLPGLKVNVKLQPHTQKLALEKKKEYEMSLSRWLPDYPDPMTYLEVFLSGSSVNNTEYANPEYDALIKKIKTELGNDEKARWKALQDAEKMLLDDAVIAPVFQRGLSYLQKPYVKDLYVHQFGPATSLKWADVQK
AAAGAAAAAACAACGACAAAGCCGAAAGATGAGAAGAAAGTATTACAGTTACTAGAAACGGGTGAAATTCCATCGTTACATTCAGGGAAAGTAACAGATGCAGTATCATTTAACGTTTTAAATAACGTAATGGAAGGATTATTCCGTTTATCGAAAAATGATGAAGTGATTGAGGCTGGAGCACAAAAATATGAAGTGAGTAAAGATGGAAAAACATATACATTCCATTTGCGTGATGCGAAATGGTCTAACGGAGATCCAGTAACAGCGCAAGATTACGTATATGCTTGGAAGCAGCTTATTAACCCGGATACAGCTTCTCAATATGCATACATTGCGTACGATGTGAAAAATGCAGAGAAAATAAATAAGAAACAACTAGGGTTAGATGAATTAGGAGTAAAAGCAAAGGATGATAAAACGTTCGTTGTAGAGTTAGAACATCCTGTACCATATTTTACGAAACTACTTATTTTACCGTCCTTCTATCCAATTAACGAGAAATATGCGAAGGAGCAAGGTGATAAATATGGATTAGAAGCAAATAAAGCGGTATATAATGGGCCATTTACATTATCTGATTGGAAGCATGAAGCTAGTTTTACGATGAAGAAAAACGATAAGTATTGGGATAAAAAAGAAGTGAAGCTAGATGAAGTAAACTATCAAATTGTTAAAGAAATTTCAACTGCGGTAAATTTATATGAAACAGATAAAGTTGATAGAGCTGTCATTTCAACTGAATTCGTAGATAAATATAAAAATAATAAAGAGCTTAAACAATATACAGATCCGGTTATGTACTTCTTCCGATTCAATGAAAATGTACCGATTCTTAAAAATAAAAATGCACGTCTTGCACTAAGTACAGTGTTTGATAAGAAAGGACTTGCGACTTCATTTTTAAATGATGGTTCGGTCGCTGCAAACTATTACGTACCAAAAGGATTTTTAAAGGGTCCAGATAAAAAAGATTTTAGAAGTACGGCAGGTGAATTTAATAAAACTGATGTAAAACAGGCGAAAGAATATTGGGAAAAGGCGAAGCAAGAGACTGGTACAAATGAAGTGACGCTTGAGTTATTAAACTATGATTTAGAAAACTTCAAAAAAGTAGGAGAATATATTAAAGAGCAGCTTGAGAAAAACTTACCGGGCTTAAAGGTAAATGTGAAATTACAACCACACACGCAAAAATTAGCGCTAGAGAAGAAGAAAGAATATGAAATGTCGTTATCACGCTGGTTACCAGATTATCCAGATCCAATGACATACTTAGAAGTATTCCTTTCTGGAAGTAGTGTGAACAATACAGAATATGCAAATCCGGAATATGATGCGTTAATTAAGAAGATTAAAACCGAATTAGGTAATGATGAAAAGGCTCGCTGGAAAGCACTACAAGATGCTGAAAAAATGCTACTTGATGATGCAGTTATTGCTCCAGTATTCCAGCGCGGATTATCTTACTTACAAAAACCATACGTGAAAGATTTATATGTACATCAATTTGGTCCAGCTACAAGTTTAAAATGGGCAGATGTACAAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,917 total letters Query= IDP05319.103 Length=1956 Score E Sequences producing significant alignments: (Bits) Value wt 2798 0.0 05H_H7_IDP05319.103_T7term.seq 1186 0.0 01H_H7_IDP05319.103_v73F.seq 1005 0.0 W S H P Q F E K G T E N L Y F Q S N A K E K T T T K P K D E Query_1 145 TGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCAAAGAAAAAACAACGACAAAGCCGAAAGATGAG 234 2 1 ................................. 33 0 42 ...........A........G....------................CA.-...A................................... 124 K K V L Q L L E T G E I P S L H S G K V T D A V S F N V L N Query_1 235 AAGAAAGTATTACAGTTACTAGAAACGGGTGAAATTCCATCGTTACATTCAGGGAAAGTAACAGATGCAGTATCATTTAACGTTTTAAAT 324 2 34 .......................................................................................... 123 0 125 .......................................................................................... 214 N V M E G L F R L S K N D E V I E A G A Q K Y E V S K D G K Query_1 325 AACGTAATGGAAGGATTATTCCGTTTATCGAAAAATGATGAAGTGATTGAGGCTGGAGCACAAAAATATGAAGTGAGTAAAGATGGAAAA 414 2 124 .......................................................................................... 213 0 215 ......N...........................................N..............N........................ 304 T Y T F H L R D A K W S N G D P V T A Q D Y V Y A W K Q L I Query_1 415 ACATATACATTCCATTTGCGTGATGCGAAATGGTCTAACGGAGATCCAGTAACAGCGCAAGATTACGTATATGCTTGGAAGCAGCTTATT 504 2 214 .......................................................................................... 303 0 305 .......................................................................................... 394 N P D T A S Q Y A Y I A Y D V K N A E K I N K K Q L G L D E Query_1 505 AACCCGGATACAGCTTCTCAATATGCATACATTGCGTACGATGTGAAAAATGCAGAGAAAATAAATAAGAAACAACTAGGGTTAGATGAA 594 2 304 .......................................................................................... 393 0 395 ....................................N...............................N........T......N..... 484 L G V K A K D D K T F V V E L E H P V P Y F T K L L I L P S Query_1 595 TTAGGAGTAAAAGCAAAGGATGATAAAACGTTCGTTGTAGAGTTAGAACATCCTGTACCATATTTTACGAAACTACTTATTTTACCGTCC 684 2 394 .......................................................................................... 483 0 485 ...N...................................N.................................................. 574 F Y P I N E K Y A K E Q G D K Y G L E A N K A V Y N G P F T Query_1 685 TTCTATCCAATTAACGAGAAATATGCGAAGGAGCAAGGTGATAAATATGGATTAGAAGCAAATAAAGCGGTATATAATGGGCCATTTACA 774 2 484 .......................................................................................... 573 0 575 ................................................................... 641 L S D W K H E A S F T M K K N D K Y W D K K E V K L D E V N Query_1 775 TTATCTGATTGGAAGCATGAAGCTAGTTTTACGATGAAGAAAAACGATAAGTATTGGGATAAAAAAGAAGTGAAGCTAGATGAAGTAAAC 864 2 574 .......................................................................................... 663 2 964 ........... 974 1 655 ........... 645 Y Q I V K E I S T A V N L Y E T D K V D R A V I S T E F V D Query_1 865 TATCAAATTGTTAAAGAAATTTCAACTGCGGTAAATTTATATGAAACAGATAAAGTTGATAGAGCTGTCATTTCAACTGAATTCGTAGAT 954 2 664 .......................................................................................... 753 K Y K N N K E L K Q Y T D P V M Y F F R F N E N V P I L K N Query_1 955 AAATATAAAAATAATAAAGAGCTTAAACAATATACAGATCCGGTTATGTACTTCTTCCGATTCAATGAAAATGTACCGATTCTTAAAAAT 1044 2 754 .......................................................................................... 843 K N A R L A L S T V F D K K G L A T S F L N D G S V A A N Y Query_1 1045 AAAAATGCACGTCTTGCACTAAGTACAGTGTTTGATAAGAAAGGACTTGCGACTTCATTTTTAAATGATGGTTCGGTCGCTGCAAACTAT 1134 2 844 .......................................................................................... 933 1 725 ..........................N........N... 687 Y V P K G F L K G P D K K D F R S T A G E F N K T D V K Q Query_1 1135 TACGTACCAAAAGG-ATTTTTAAAGGGTCCAGATAAAAAAGATTTTAGAAGTACGGCAGGTGAATTTAATAAAACTGATGTAAAACAGGC 1223 2 934 ..............-........................................................................... 1022 2 631 ........... 641 1 686 ..............N........................................................................... 597 A K E Y W E K A K Q E T G T N E V T L E L L N Y D L E N F K Query_1 1224 GAAAGAATATTGGGAAAAGGCGAAGCAAGAGACTGGTACAAATGAAGTGACGCTTGAGTTATTAAACTATGATTTAGAAAACTTCAAAAA 1313 2 1023 .......................................................................................... 1112 1 596 ..................................................................N..N.................... 507 K V G E Y I K E Q L E K N L P G L K V N V K L Q P H T Q K L Query_1 1314 AGTAGGAGAATATATTAAAGAGCAGCTTGAGAAAAACTTACCGGGCTTAAAGGTAAATGTGAAATTACAACCACACACGCAAAAATTAGC 1403 2 1113 .......................................................................................... 1202 1 506 .......................................................................................... 417 A L E K K K E Y E M S L S R W L P D Y P D P M T Y L E V F L Query_1 1404 GCTAGAGAAGAAGAAAGAATATGAAATGTCGTTATCACGCTGGTTACCAGATTATCCAGATCCAATGACATACTTAGAAGTATTCCTTTC 1493 2 1203 .......................................................................................... 1292 1 416 .......................................................................................... 327 S G S S V N N T E Y A N P E Y D A L I K K I K T E L G N D E Query_1 1494 TGGAAGTAGTGTGAACAATACAGAATATGCAAATCCGGAATATGATGCGTTAATTAAGAAGATTAAAACCGAATTAGGTAATGATGAAAA 1583 2 1293 .......................................................................................... 1382 1 326 .......................................................................................... 237 K A R W K A L Q D A E K M L L D D A V I A P V F Q R G L S Y Query_1 1584 GGCTCGCTGGAAAGCACTACAAGATGCTGAAAAAATGCTACTTGATGATGCAGTTATTGCTCCAGTATTCCAGCGCGGATTATCTTACTT 1673 2 1383 .......................................................................................... 1472 1 236 .......................................................................................... 147 L Q K P Y V K D L Y V H Q F G P A T S L K W A D V Q K * H W Query_1 1674 ACAAAAACCATACGTGAAAGATTTATATGTACATCAATTTGGTCCAGCTACAAGTTTAAAATGGGCAGATGTACAAAAATAACATTGGAA 1763 2 1473 ............................................................................... 1551 1 146 ...........................................................T..-........................... 58 K W I T D P N S S S V D K L A A A L E H H H H H H * D P A A Query_1 1764 GTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAA 1853 1 57 ........ 50 1 47 ............. 35 N K A R K E A E L A A A T A E Q * L A Query_1 1854 C-AAAGCCCG-AAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCAT 1909 2 1444 ...........-.... 1430 1 34 .A........A....... 17 1 175 ...........-.... 189 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5565860 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 2,917 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP05404.103 (IDP05404.22-314.P(314)..pMCSG73)
ACSNKEEKKADAKDAKTEERTVQHAKGEIKIPANPKKIADLSGSTEELLIFGMKPIITANTSQEKIDAHIEKKLKDVKPVGSAWGDKINIEAVAAAKPDLILVNNRQEKIYDQLSKIAPTVMLKTPLDQWRPKFEEVGQIFGKEKETKEWFKQYDEKASKLHDKIVAKTGDAKFMKMAAYPNAFRVYGDYGYGSVIFNDLKLPAVKGTPTDKPLVQVQKEALIDYNPDYLFVFTTGDGSQRLKEFQEESIWKNMNAVKNNHVFTIKNEELNKGYFPLGKEMILDEVAEFVLGK
GCTTGTTCTAACAAAGAAGAAAAGAAAGCAGATGCGAAGGATGCGAAGACTGAAGAGCGCACTGTACAACATGCAAAAGGGGAAATTAAAATCCCTGCAAATCCAAAGAAAATTGCTGACCTTAGTGGTTCAACAGAAGAATTATTAATCTTTGGTATGAAACCAATTATTACTGCTAATACATCTCAAGAAAAAATTGATGCACATATTGAAAAGAAATTAAAAGACGTAAAACCAGTTGGTTCTGCTTGGGGCGATAAAATTAACATCGAAGCAGTAGCTGCTGCAAAACCAGACTTAATTCTTGTAAACAATCGTCAAGAAAAGATTTATGATCAATTATCTAAAATTGCACCAACAGTTATGTTAAAAACTCCATTAGACCAATGGCGTCCAAAATTCGAAGAAGTAGGTCAAATCTTCGGTAAAGAGAAAGAAACAAAAGAATGGTTCAAACAGTATGATGAAAAAGCAAGCAAATTACATGACAAAATCGTTGCGAAAACTGGCGATGCTAAATTCATGAAAATGGCTGCATATCCAAATGCTTTCCGTGTATATGGTGACTACGGTTACGGTAGCGTAATCTTTAATGATTTAAAATTACCAGCAGTTAAAGGTACACCAACTGATAAACCGTTAGTACAAGTGCAAAAAGAAGCTTTAATTGATTACAACCCAGATTACTTATTCGTATTTACGACTGGTGACGGTTCTCAGCGCCTAAAAGAATTCCAAGAAGAATCAATTTGGAAAAATATGAACGCTGTTAAAAACAACCACGTATTTACAATTAAAAACGAAGAATTAAACAAAGGTTACTTCCCACTAGGTAAAGAAATGATCTTAGATGAAGTTGCTGAGTTCGTTTTAGGGAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,436 total letters Query= IDP05404.103 Length=1284 Score E Sequences producing significant alignments: (Bits) Value wt 1586 0.0 02D_D8_IDP05404.103_v73F.seq 1550 0.0 06D_D8_IDP05404.103_T7term.seq 930 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 0 41 ...........N........G....------....................T.. 88 0 25 ...C........... 39 0 26 .............. 39 A A C S N K E E K K A D A K D A K T E E R T V Q H A K G E I Query_1 199 GCCGCTTGTTCTAACAAAGAAGAAAAGAAAGCAGATGCGAAGGATGCGAAGACTGAAGAGCGCACTGTACAACATGCAAAAGGGGAAATT 288 2 1 ....................................................................................... 87 0 89 .................-........................................................................ 177 K I P A N P K K I A D L S G S T E E L L I F G M K P I I T A Query_1 289 AAAATCCCTGCAAATCCAAAGAAAATTGCTGACCTTAGTGGTTCAACAGAAGAATTATTAATCTTTGGTATGAAACCAATTATTACTGCT 378 2 88 .......................................................................................... 177 0 178 .......................................................................................... 267 N T S Q E K I D A H I E K K L K D V K P V G S A W G D K I N Query_1 379 AATACATCTCAAGAAAAAATTGATGCACATATTGAAAAGAAATTAAAAGACGTAAAACCAGTTGGTTCTGCTTGGGGCGATAAAATTAAC 468 2 178 .......................................................................................... 267 0 268 .......................................................................................... 357 I E A V A A A K P D L I L V N N R Q E K I Y D Q L S K I A P Query_1 469 ATCGAAGCAGTAGCTGCTGCAAAACCAGACTTAATTCTTGTAAACAATCGTCAAGAAAAGATTTATGATCAATTATCTAAAATTGCACCA 558 2 268 .......................................................................................... 357 0 358 .......................................................................................... 447 T V M L K T P L D Q W R P K F E E V G Q I F G K E K E T K Query_1 559 ACAGTTATGTTAAAAACTCCATTAGACCAATGGCGTCCAAAATTCGAAGAAGTAGGTCAAA-TCTTCGGTAAAGAGAAAGAAACAAAAGA 647 2 358 .............................................................-............................ 446 0 448 ..................................N................-.........-............................ 535 1 567 .......................................A............................ 500 E W F K Q Y D E K A S K L H D K I V A K T G D A K F M K M A Query_1 648 ATGGTTCAAACAGTATGATGAAAAAGCAAGCAAATTACATGACAAAATCGTTGCGAAAACTGGCGATGCTAAATTCATGAAAATGGCTGC 737 2 447 .......................................................................................... 536 0 536 ...........................N.............................................................. 625 1 499 ........................................N................................................. 410 A Y P N A F R V Y G D Y G Y G S V I F N D L K L P A V K G T Query_1 738 ATATCCAAATGCTTTCCGTGTATATGGTGACTACGGTTACGGTAGCGTAATCTTTAATGATTTAAAATTACCAGCAGTTAAAGGTACACC 827 2 537 .......................................................................................... 626 0 626 .......................................................................................... 715 1 409 .......................................................................................... 320 P T D K P L V Q V Q K E A L I D Y N P D Y L F V F T T G D G Query_1 828 AACTGATAAACCGTTAGTACAAGTGCAAAAAGAAGC-TTTAATTGATTACAACCCAGATTACTTATTCGTATTTACGACTGGTGACGGTT 916 2 627 ....................................-..................................................... 715 0 716 ..................N.................T.............-....T.................................. 804 1 319 ....................................-..................................................... 231 S Q R L K E F Q E E S I W K N M N A V K N N H V F T I K N E Query_1 917 CTCAGCGCCTAAAAGAATTCCAAGAAGAATCAATTTGGAAAAATATGAACGCTGTTAAAAACAACCACGTATTTACAATTAAAAACGAAG 1006 2 716 .......................................................................................... 805 0 805 .........................N..................................C.......N.................N..N 894 1 230 .......................................................................................... 141 E L N K G Y F P L G K E M I L D E V A E F V L G K * H W Query_1 1007 AATTAAACAAAGGTTACTT-CCCACTAGGTAAAGAAA-TGATCTTAGATGAAGTTG-CTGAGTTCGTTTTAGGG-AAATAACATTGG-AA 1091 2 806 ...................-.................-..................-.................-... 879 0 895 ....-...C..........C..........C.N....T..................C.................A.....N.N....N.. 983 1 140 ...................-.................-..................-.................-............-.. 56 K W I T D P N S S S V D K L A A A L E H H H H H H * D P A A Query_1 1092 G-TGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTA 1180 0 984 .N..... 990 1 55 .-........--..... 42 1 46 ............ 35 N K A R K E Query_1 1181 A--CAAAGCCCG-AAAGGAA 1197 1 34 .ACA........A....... 15 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3034179 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 2,436 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP05678.103 (IDP05678.20-256.P(256)..pMCSG73)
EDTPEKVQTTTIHISAAASLKDSIDDVKPLFEKANPTIKLSFDFGGSGQIRERVESGAPIDGVLLASKKDADTLIKQNLAEKTKEFAGNELVLIEPKNVDQKTEANLEQLLNDASKIAIGDPESVPAGAYAKQTLENLNLYNAEKAKLVLATDVRQVLSYVEAGNADAGFVYQTDALLSKKVQVKAKIDEKLHDPIAYYSAQVSDSDKKEETATFLDFMNKSEAQKILEKYGFKAAN
GAGGATACGCCTGAGAAAGTCCAAACAACGACTATACATATTTCAGCAGCAGCAAGTTTAAAAGATTCGATAGACGACGTAAAACCACTTTTTGAAAAAGCCAATCCAACGATTAAATTATCTTTTGATTTTGGTGGTTCTGGACAAATTCGCGAACGTGTCGAAAGCGGGGCACCAATTGACGGCGTCCTTTTAGCGAGTAAAAAAGATGCAGATACACTGATTAAGCAAAATTTAGCAGAAAAAACGAAAGAATTTGCGGGAAATGAACTCGTTTTAATTGAACCTAAAAACGTGGATCAGAAGACAGAGGCGAACTTAGAACAATTACTAAATGACGCATCCAAAATCGCCATCGGGGACCCAGAATCAGTACCAGCAGGTGCTTATGCCAAACAAACGCTCGAAAACCTAAATCTATATAATGCAGAAAAAGCAAAGCTTGTTCTTGCAACCGATGTTCGTCAAGTCTTATCCTACGTGGAAGCCGGAAATGCAGATGCAGGCTTCGTTTACCAGACAGATGCTCTATTAAGCAAGAAAGTACAAGTGAAGGCAAAAATCGATGAGAAACTCCATGATCCAATCGCTTACTACAGCGCACAAGTCAGTGATTCAGATAAAAAAGAAGAAACCGCTACATTTCTTGATTTTATGAATAAATCAGAAGCGCAAAAAATACTGGAAAAATACGGATTCAAAGCAGCAAAC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,392 total letters Query= IDP05678.103 Length=1116 Score E Sequences producing significant alignments: (Bits) Value 07F_F9_IDP05678.103_T7term.seq 1371 0.0 03F_F9_IDP05678.103_v73F.seq 1314 0.0 wt 1283 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 1 828 ...........A........G....------....................... 781 1 846 .....C........... 830 0 42 ...........A........G....------....................... 89 A E D T P E K V Q T T T I H I S A A A S L K D S I D D V K P Query_1 199 GCCGAGGATACGCCTGAGAAAGTCCAAACAACGACTATACATATTTCAGCAGCAGCAAGTTTAAAAGATTCGATAGACGACGTAAAACCA 288 1 780 .......................................................................................... 691 0 90 .......................................................................................... 179 2 1 ....................................................................................... 87 L F E K A N P T I K L S F D F G G S G Q I R E R V E S G A Query_1 289 -CTTTTTGAAAAAGCCAATCCAACGATTAAATT-ATCTTTTGATTTTGGTGGTTCTGGACAAATTCGCGAACGTGTCGAAAGCGGGGCAC 376 1 690 A................................T............N........................................... 601 0 180 -................................-........................................................ 267 2 88 -................................-........................................................ 175 P I D G V L L A S K K D A D T L I K Q N L A E K T K E F A G Query_1 377 CAATTGACGGCGTCCTTTTAGCGAGTAAAAAAGATGCAGATACACTGATTAAGCAAAATTTAGCAGAAAAAACGAAAGAATTTGCGGGAA 466 1 600 .......................................................................................... 511 0 268 .......................................................................................... 357 2 176 .......................................................................................... 265 N E L V L I E P K N V D Q K T E A N L E Q L L N D A S K I A Query_1 467 ATGAACTCGTTTTAATTGAACCTAAAAACGTGGATCAGAAGACAGAGGCGAACTTAGAACAATTACTAAATGACGCATCCAAAATCGCCA 556 1 510 .......................................................................................... 421 0 358 .................................................N........................................ 447 2 266 .......................................................................................... 355 I G D P E S V P A G A Y A K Q T L E N L N L Y N A E K A K L Query_1 557 TCGGGGACCCAGAATCAGTACCAGCAGGTGCTTATGCCAAACAAACGCTCGAAAACCTAAATCTATATAATGCAGAAAAAGCAAAGCTTG 646 1 420 .......................................................................................... 331 0 448 .......................................................................................... 537 2 356 .......................................................................................... 445 V L A T D V R Q V L S Y V E A G N A D A G F V Y Q T D A L L Query_1 647 TTCTTGCAACCGATGTTCGTCAAGTCTTATCCTACGTGGAAGCCGGAAATGCAGATGCAGGCTTCGTTTACCAGACAGATGCTCTATTAA 736 1 330 .......................................................................................... 241 0 538 .....................................................N.................................... 627 2 446 .......................................................................................... 535 S K K V Q V K A K I D E K L H D P I A Y Y S A Q V S D S D K Query_1 737 GCAAGAAAGTACAAGTGAAGGCAAAAATCGATGAGAAACTCCATGATCCAATCGCTTACTACAGCGCACAAGTCAGTGATTCAGATAAAA 826 1 240 .......................................................................................... 151 0 628 ..................................................................................N....... 717 2 536 .......................................................................................... 625 K E E T A T F L D F M N K S E A Q K I L E K Y G F K A A N Query_1 827 AAGAAGAAACCGCTACATTTC-TTGATTTTATGAATAAATCAGAAGCGCAAAAAA-TACTGGAAAAATACGGATTCAAAGCAGCAAACTA 914 1 150 .....................-.................................-.................................. 63 0 718 .....................T.................................T....................-........ 801 2 626 .....................-.................................-................................ 711 * H W K W I T D P N S S S V D K L A A A L E H H H H H H * D Query_1 915 ACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCC 1004 1 62 ..................--..... 40 1 44 .... 41 P A A N K A R K E Query_1 1005 GGCTGCTAACAAAGCCCGAAAGGAA 1029 1 40 ......................... 16 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2584047 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 2,392 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP05684.103 (IDP05684.1-288.F..pMCSG73)
MTKSRFFSDVAETSSFVFAVAGADDEVVLETIRLALKQKLGKFLLFGKKEDKTLTANESVTWIQTDTAEAAAQGAILAVKNKEADILVKGFIPTATLMHHVLKKENGLRTDQLLSQIAIFDIPTYHKPLLITDCAMNVAPKTKEKIAITENALAVAHQIGITNPKIALLSAVEEVTAKMPSTLEAQEVVQHFGNQISVSGPLALDVAISKEAALHKGITDSSAGEADILIAPNIETGNALYKSLVYFAGAKVGSAVVGAKVPIVISSRNDSPENKLASFILTVRLVEK
ATGACAAAAAGCAGATTTTTTTCAGATGTAGCAGAAACGAGTTCATTTGTTTTTGCAGTTGCCGGCGCAGATGATGAGGTGGTATTAGAAACCATTCGTCTTGCATTAAAACAAAAACTAGGTAAATTTCTTTTATTTGGTAAGAAAGAAGACAAAACCTTAACAGCCAACGAAAGTGTTACGTGGATTCAAACTGATACCGCAGAAGCTGCAGCGCAGGGTGCCATTTTAGCTGTAAAAAATAAAGAAGCAGACATTTTAGTAAAAGGATTCATCCCAACAGCTACACTGATGCACCACGTATTAAAAAAAGAAAATGGCCTTCGAACTGACCAATTATTAAGTCAAATTGCTATTTTCGACATTCCGACGTATCACAAGCCATTATTAATAACTGATTGTGCCATGAACGTAGCACCAAAAACAAAGGAAAAAATTGCTATCACAGAAAATGCCTTAGCCGTAGCTCATCAGATTGGTATCACCAACCCTAAAATCGCTTTACTTAGTGCAGTAGAAGAAGTGACTGCAAAAATGCCATCTACATTGGAGGCGCAGGAAGTAGTACAGCATTTTGGAAACCAAATATCCGTCTCAGGTCCGCTAGCTCTCGATGTAGCTATTTCCAAAGAAGCGGCTCTCCATAAAGGAATTACGGATAGTTCAGCGGGAGAAGCAGACATATTAATTGCTCCTAATATTGAAACAGGAAATGCACTTTATAAATCATTAGTCTACTTTGCTGGAGCTAAAGTAGGTAGTGCAGTGGTCGGAGCAAAAGTACCTATTGTTATTTCCTCAAGAAATGATTCACCGGAAAACAAATTAGCTTCATTCATACTAACTGTAAGACTAGTTGAGAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,006 total letters Query= IDP05684.103 Length=1269 Score E Sequences producing significant alignments: (Bits) Value 09E_B11_IDP05684.103_v73F.seq 1582 0.0 wt 1559 0.0 09B_B11_IDP05684.103_T7term.seq 1398 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 1 44 ...........A........G....------...................N... 91 1 28 ............ 39 A M T K S R F F S D V A E T S S F V F A V A G A D D E V V L Query_1 199 GCCATGACAAAAAGCAGAtttttttCAGATGTAGCAGAAACGAGTTCATTTGTTTTTGCAGTTGCCGGCGCAGATGATGAGGTGGTATTA 288 1 92 .......................................................................................... 181 2 1 ....................................................................................... 87 0 843 . 843 E T I R L A L K Q K L G K F L L F G K K E D K T L T A N E Query_1 289 GAA-ACCATTCGTCTTGCATTAAAACAAAAACTAGGTAAATTTCTTTTATTTGGTAAGAAAGAAGACAAAACCTTAACAGCCAACGAAAG 377 1 182 ...-...................................................................................... 270 2 88 ...-...................................................................................... 176 0 842 ...N....................................A................................................. 753 S V T W I Q T D T A E A A A Q G A I L A V K N K E A D I L V Query_1 378 TGTTACGTGGATTCAAACTGATACCGCAGAAGCTGCAGCGCAGGGTGCCATTTTAGCTGTAAAAAAT-AAAGAAGCAGACATTTTAGTAA 466 1 271 ...................................................................-...................... 359 1 767 ............A... 782 2 177 ...................................................................-...................... 265 2 672 ............A... 687 0 752 ...................................................................G..........N........... 663 0 256 ............A... 241 K G F I P T A T L M H H V L K K E N G L R T D Q L L S Q I A Query_1 467 AAGGATTCATCCCAACAGCTACACTGATGCACCACGTATTaaaaaaaGAAAATGGCCTTCGAACTGACCAATTATTAAGTCAAATTGCTA 556 1 360 .......................................................................................... 449 2 266 .......................................................................................... 355 0 662 .......................................................................................... 573 I F D I P T Y H K P L L I T D C A M N V A P K T K E K I A I Query_1 557 TTTTCGACATTCCGACGTATCACAAGCCATTATTAATAACTGATTGTGCCATGAACGTAGCACCAAAAACAAAGGAAAAAATTGCTATCA 646 1 450 .......................................................................................... 539 2 356 .......................................................................................... 445 0 572 .......................................................................................... 483 T E N A L A V A H Q I G I T N P K I A L L S A V E E V T A K Query_1 647 CAGAAAATGCCTTAGCCGTAGCTCATCAGATTGGTATCACCAACCCTAAAATCGCTTTACTTAGTGCAGTAGAAGAAGTGACTGCAAAAA 736 1 540 .......................................................................................... 629 2 446 .......................................................................................... 535 0 482 .......................................................................................... 393 M P S T L E A Q E V V Q H F G N Q I S V S G P L A L D V A I Query_1 737 TGCCATCTACATTGGAGGCGCAGGAAGTAGTACAGCATTTTGGAAACCAAATATCCGTCTCAGGT-CCGCTAGCTCTCGATGTAGCTATT 825 1 630 .................................................................C........................ 719 2 536 .................................................................-........................ 624 0 392 .................................................................-........................ 304 S K E A A L H K G I T D S S A G E A D I L I A P N I E T G N Query_1 826 TCCAAAGAAGCGGCTCTCCATAAAGGAATTACGGATAGTTCAGCGGGAGAAGCAGACATATTAATTGCTCCTAATATTGAAACAGGAAAT 915 1 720 .......................................................................................... 809 1 340 ............T... 355 2 625 .......................................................................................... 714 2 246 ............T... 261 0 303 .......................................................................................... 214 A L Y K S L V Y F A G A K V G S A V V G A K V P I V I S S Query_1 916 GCACTTTATAAATCATTAGTCTACTTTGCTGGAGCTAAAGTAGGTAGTGCAGTGGTCGGAGCAAAAGTACCTA-TTGTTA-TTTCCTCAA 1003 1 810 .........................................................................T......N........G 899 2 715 .........................................................................-......-......... 802 0 213 .........................................................................-......-......... 126 R N D S P E N K L A S F I L T V R L V E K * H W K W I T Query_1 1004 GAAATGATTCA-CCGGAAAACAAATTAGC-TTCA-TTCATACTAAC-TGTAAGACTAGTTGAGAAATAACATTGGAA-GTGGATAACGGA 1088 1 900 A..........C.................T....T...N.......T.......NN.....................N...... 983 2 803 ...........-.................-....-...........-................... 864 0 125 ...........-.................-....-.....N..TT.-.-............................-.........--. 44 D P N S S S V D K L A A A L E H H H H H H * D P A A N K A R Query_1 1089 TCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCG--A 1176 1 988 .....................NGA. 1012 0 43 .... 40 0 44 .....................N--. 22 K E A E L A A A T A E Q * L A * P L G A S K R V L R Query_1 1177 AAGGAAGCTGAG-TTGGCTGCT-GCCACCGC-TGAGCAATAACTAGC-ATAACCCC--TTGGGG--CCTCTAAACGGGT-CTTG-AGGGG 1256 1 1013 ......N.....T.........N........T.N.N.N...C.....NN.......CT......GCT............N....N..... 1102 0 21 ..A... 16 G F Query_1 1257 TTTTTT 1262 1 1103 ...... 1108 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3706374 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 3,006 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP09018.106 (IDP09018.1-369.F..pMCSG68)
MSQQVTQLNPTQQTTQSAFLATTVITAQCHAILNTQFTPPTVKPDWFDDLSKKLDSAKLVAKQWIDDLGPQVSASIPSSVINFDATFQASIDAIHELYKADPTASGKDNTTVQQASQIMTALSSQVSGIEATVKGMNKELSDWGVKMQAAHDDLVNGATNIQKTIIDLQTDIESMNNAIDNNRAAIEKLNKDLVYAQVAVGVGIFMLVAGVALTVATAGTAAAVSGGIAAVGAASIIAGGVTWGVLQNQIDDDYDSIAQEQKQKAEDQQQIIALQGLSNASSAVVSAIETSTSVLSDFETTWTVFGNELDDVVTKLNNGASMQSIIMEKVMSDAAKNEWDDAVELAKQLASAKIAIETKELAPAVKQAA
ATGTCACAACAAGTTACTCAGTTGAACCCAACACAGCAAACCACTCAATCTGCATTTTTGGCAACCACAGTCATCACAGCGCAGTGCCATGCCATTTTAAACACACAATTTACGCCACCTACGGTAAAACCAGATTGGTTTGATGACCTGAGTAAAAAGTTAGACTCAGCAAAATTGGTTGCTAAGCAATGGATTGACGATTTGGGACCACAAGTATCAGCATCGATTCCCTCCAGCGTAATTAATTTTGACGCTACTTTTCAAGCATCAATCGATGCCATTCATGAGTTATATAAAGCGGATCCAACAGCAAGTGGCAAAGACAATACAACGGTTCAGCAAGCTTCACAGATTATGACCGCCCTATCTTCTCAAGTCAGCGGTATCGAAGCCACAGTCAAAGGGATGAATAAGGAACTCTCTGATTGGGGGGTGAAAATGCAAGCAGCACATGATGACTTAGTCAATGGTGCGACCAATATTCAAAAAACAATTATTGATTTACAAACAGATATTGAAAGTATGAATAACGCAATAGACAACAATCGTGCAGCCATTGAGAAACTGAATAAAGATCTGGTCTATGCACAAGTTGCGGTTGGTGTTGGCATCTTTATGCTCGTCGCAGGCGTTGCGCTTACCGTCGCAACCGCTGGCACCGCAGCTGCAGTCTCCGGCGGTATCGCAGCCGTCGGCGCGGCATCGATTATTGCTGGTGGCGTTACATGGGGCGTATTACAAAATCAAATTGATGATGACTACGACAGCATCGCGCAAGAACAAAAGCAAAAAGCTGAAGATCAACAACAGATTATCGCTTTACAGGGGCTTTCCAATGCCAGTAGCGCCGTTGTTTCAGCGATTGAAACATCGACATCAGTATTATCTGATTTTGAAACAACGTGGACTGTCTTTGGCAACGAGTTAGATGATGTAGTAACAAAGCTTAATAATGGTGCATCCATGCAGAGCATTATAATGGAAAAAGTGATGTCTGACGCAGCGAAAAACGAATGGGATGATGCGGTTGAACTTGCTAAACAGCTAGCTAGCGCAAAAATAGCTATCGAAACGAAAGAGTTAGCACCTGCAGTAAAACAAGCAGCT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 2 sequences; 2,371 total letters Query= IDP09018.106 Length=1512 Score E Sequences producing significant alignments: (Bits) Value wt 1997 0.0 09F_D5_IDP09018.106_T7short.seq 1889 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 58 TTCCCCTC-TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTG 146 0 18 ........C...........................................................N..................... 107 W S H P Q F E K G T E N L Y F Q S N A M S Q Q V T Q L N P T Query_1 147 GTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGTCACAACAAGTTACTCAGTTGAACCCAACACA 236 1 1 ................................... 35 0 108 .......................................................................................... 197 Q Q T T Q S A F L A T T V I T A Q C H A I L N T Q F T P P T Query_1 237 GCAAACCACTCAATCTGCATTTTTGGCAACCACAGTCATCACAGCGCAGTGCCATGCCATTTTAAACACACAATTTACGCCACCTACGGT 326 1 36 .......................................................................................... 125 0 198 .......................................................................................... 287 V K P D W F D D L S K K L D S A K L V A K Q W I D D L G P Q Query_1 327 AAAACCAGATTGGTTTGATGACCTGAGTAAAAAGTTAGACTCAGCAAAATTGGTTGCTAAGCAATGGATTGACGATTTGGGACCACAAGT 416 1 126 .......................................................................................... 215 0 288 .......................................................................................... 377 V S A S I P S S V I N F D A T F Q A S I D A I H E L Y K A D Query_1 417 ATCAGCATCGATTCCCTCCAGCGTAATTAATTTTGACGCTACTTTTCAAGCATCAATCGATGCCATTCATGAGTTATATAAAGCGGATCC 506 1 216 .......................................................................................... 305 0 378 .......................................................................................... 467 P T A S G K D N T T V Q Q A S Q I M T A L S S Q V S G I E A Query_1 507 AACAGCAAGTGGCAAAGACAATACAACGGTTCAGCAAGCTTCACAGATTATGACCGCCCTATCTTCTCAAGTCAGCGGTATCGAAGCCAC 596 1 306 .......................................................................................... 395 0 468 .......................................................................................... 557 T V K G M N K E L S D W G V K M Q A A H D D L V N G A T N I Query_1 597 AGTCAAAGGGATGAATAAGGAACTCTCTGATTGGGGGGTGAAAATGCAAGCAGCACATGATGACTTAGTCAATGGTGCGACCAATATTCA 686 1 396 .......................................................................................... 485 0 558 ..................................................T....................................... 647 Q K T I I D L Q T D I E S M N N A I D N N R A A I E K L N K Query_1 687 AAAAACAATTATTGATTTACAAACAGATATTGAAAGTATGAATAACGCAATAGACAACAATCGTGCAGCCATTGAGAAACTGAATAAAGA 776 1 486 .......................................................................................... 575 0 648 .......................................................................................... 737 D L V Y A Q V A V G V G I F M L V A G V A L T V A T A G T A Query_1 777 TCTGGTCTATGCACAAGTTGCGGTTGGTGTTGGCATCTTTATGCTCGTCGCAGGCGTTGCGCTTACCGTCGCAACCGCTGGCACCGCAGC 866 1 576 .......................................................................................... 665 0 738 .......................................................................................... 827 A A V S G G I A A V G A A S I I A G G V T W G V L Q N Q I D Query_1 867 TGCAGTCTCCGGCGGTATCGCAGCCGTCGGCGCGGCATCGATTATTGCTGGTGGCGTTACAT-GGGGCGTATTACAAAATCAAATTGATG 955 1 666 ..............................................................-........................... 754 0 828 ..............................................................G........................... 917 D D Y D S I A Q E Q K Q K A E D Q Q Q I I A L Q G L S N A Query_1 956 ATGACTACGACAGCATCGCGCAAGAACAAAAGCAAAAAGCTG-AAGATCAACAACAGATTATCGCTTTA-CAGGGGCTTTCCAATGCCAG 1043 1 755 ..........................................-..........................-.................... 842 0 918 .................................-N.......A..........................C............N....... 1006 S S A V V S A I E T S T S V L S D F E T T W T V F G N E L D Query_1 1044 TAGCGCCGTTGTTTCAGCGATTGAAACATCGACATCAGTATTATCTGATTTTGAAACAACGTGG-ACTGTCTTTGGCAACGAGTTAGATG 1132 1 843 ................................................................-......................... 931 0 1007 .........N.-..N........-........................-...........N...A............-....AG.N.... 1092 D V V T K L N N G A S M Q S I I M E K V M S D A A K N E W Query_1 1133 ATGTAGTAACAAAGCTTAATAAT-GGTGCATCCATG--CAGAGCATTATAA-TGGAAAAAGTGATGTCTGACGCAGCGAAAAACGAATGG 1218 1 932 .......................-............--.............-...................................... 1017 0 1093 .......................N.........N..NC.GA......N...T..NN...C.N..... 1159 D D A V E L A K Q L A S A K I A I E T K E L A P A V K Q A A Query_1 1219 GATGATGCGGTTGAACTTGCTAAACAGCTAGCTAGCGCAAAAATAGCTATCGAAACGAAAGAGTTAGCACCTGCAGTAAAACAAGCAGCT 1308 1 1018 .......................................................................................... 1107 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3499144 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 2,371 Number of sequences in database: 2 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP90822.103 (IDP90822.1-321.F..pMCSG73)
MHVLIVKTSSMGDVLHTLPALTDAMNAIPGIRFDWVVEEGFSQIPSWHPAVDKVIPVAIRRWRKNWFGSDTRQERCDFKRVVQQRSYDRVIDAQGLIKSAALITRIAKGTKHGPDCKSAREPFASWFYNCRHEIDTKQHAVERIRQLFAKSLGYDKPESYGDYAIAQRFLNQLPADAGQYLVFLHATTRDNKHWPESHWVQLIELVQPTGLKIKLPWGAEHEHQRALRLAEHFPHVEVLPKLSLQQVAEVLAGAKAVVSVDTGLSHLTAALDRPNITLFGPTDPGLIGGYGKNQIAVISEQKKMDSLSAPTIMARLETLIS
ATGCACGTATTAATCGTTAAAACCTCGTCAATGGGCGATGTTTTACATACCTTACCCGCGCTGACGGATGCCATGAATGCCATACCCGGTATTCGCTTCGATTGGGTGGTAGAAGAAGGGTTCAGCCAAATCCCCAGTTGGCATCCGGCGGTGGATAAAGTGATCCCGGTGGCCATCCGCCGTTGGCGTAAAAACTGGTTTGGTAGTGATACCCGGCAGGAACGCTGTGATTTTAAGCGTGTGGTACAACAACGCAGTTATGATCGGGTCATTGATGCCCAAGGGTTGATTAAAAGCGCCGCGCTGATAACCCGGATTGCGAAAGGCACGAAGCACGGGCCGGATTGTAAAAGTGCCCGTGAGCCGTTTGCCAGTTGGTTTTATAACTGCCGCCATGAAATTGATACCAAACAACATGCCGTAGAACGTATACGTCAACTGTTTGCTAAAAGCCTCGGCTATGATAAACCGGAAAGTTATGGCGACTATGCCATCGCACAACGCTTTTTAAATCAGTTACCGGCCGATGCAGGTCAGTACTTGGTTTTCCTACATGCGACGACCCGTGACAACAAGCACTGGCCCGAGAGCCATTGGGTACAATTGATTGAGTTAGTGCAACCCACAGGTTTAAAAATCAAACTACCGTGGGGCGCAGAACATGAGCATCAGCGCGCTTTACGATTAGCGGAACATTTCCCACACGTAGAGGTTCTGCCTAAGCTCAGTCTTCAGCAGGTCGCCGAGGTTCTGGCGGGTGCAAAAGCCGTAGTCTCTGTCGATACTGGCCTCAGCCATCTGACCGCCGCCCTGGATCGCCCAAACATTACGCTGTTTGGCCCCACTGACCCCGGTTTAATCGGCGGCTACGGGAAAAACCAAATTGCCGTGATTTCCGAGCAAAAAAAGATGGATAGCCTCTCTGCGCCCACTATTATGGCACGATTAGAAACACTCATTTCA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 973 total letters Query= IDP90822.103 Length=1368 Score E Sequences producing significant alignments: (Bits) Value wt 1737 0.0 M H V L I V K T S S M G D V L H T L P A L T D A M N A I P G Query_1 202 ATGCACGTATTAATCGTTAAAACCTCGTCAATGGGCGATGTTTTACATACCTTACCCGCGCTGACGGATGCCATGAATGCCATACCCGGT 291 2 1 .......................................................................................... 90 I R F D W V V E E G F S Q I P S W H P A V D K V I P V A I R Query_1 292 ATTCGCTTCGATTGGGTGGTAGAAGAAGGGTTCAGCCAAATCCCCAGTTGGCATCCGGCGGTGGATAAAGTGATCCCGGTGGCCATCCGC 381 2 91 .......................................................................................... 180 R W R K N W F G S D T R Q E R C D F K R V V Q Q R S Y D R V Query_1 382 CGTTGGCGTAAAAACTGGTTTGGTAGTGATACCCGGCAGGAACGCTGTGATTTTAAGCGTGTGGTACAACAACGCAGTTATGATCGGGTC 471 2 181 .......................................................................................... 270 I D A Q G L I K S A A L I T R I A K G T K H G P D C K S A R Query_1 472 ATTGATGCCCAAGGGTTGATTAAAAGCGCCGCGCTGATAACCCGGATTGCGAAAGGCACGAAGCACGGGCCGGATTGTAAAAGTGCCCGT 561 2 271 .......................................................................................... 360 E P F A S W F Y N C R H E I D T K Q H A V E R I R Q L F A K Query_1 562 GAGCCGTTTGCCAGTTGGTTTTATAACTGCCGCCATGAAATTGATACCAAACAACATGCCGTAGAACGTATACGTCAACTGTTTGCTAAA 651 2 361 .......................................................................................... 450 S L G Y D K P E S Y G D Y A I A Q R F L N Q L P A D A G Q Y Query_1 652 AGCCTCGGCTATGATAAACCGGAAAGTTATGGCGACTATGCCATCGCACAACGCTTTTTAAATCAGTTACCGGCCGATGCAGGTCAGTAC 741 2 451 .......................................................................................... 540 L V F L H A T T R D N K H W P E S H W V Q L I E L V Q P T G Query_1 742 TTGGTTTTCCTACATGCGACGACCCGTGACAACAAGCACTGGCCCGAGAGCCATTGGGTACAATTGATTGAGTTAGTGCAACCCACAGGT 831 2 541 .......................................................................................... 630 L K I K L P W G A E H E H Q R A L R L A E H F P H V E V L P Query_1 832 TTAAAAATCAAACTACCGTGGGGCGCAGAACATGAGCATCAGCGCGCTTTACGATTAGCGGAACATTTCCCACACGTAGAGGTTCTGCCT 921 2 631 .......................................................................................... 720 K L S L Q Q V A E V L A G A K A V V S V D T G L S H L T A A Query_1 922 AAGCTCAGTCTTCAGCAGGTCGCCGAGGTTCTGGCGGGTGCAAAAGCCGTAGTCTCTGTCGATACTGGCCTCAGCCATCTGACCGCCGCC 1011 2 721 .......................................................................................... 810 L D R P N I T L F G P T D P G L I G G Y G K N Q I A V I S E Query_1 1012 CTGGATCGCCCAAACATTACGCTGTTTGGCCCCACTGACCCCGGTTTAATCGGCGGCTACGGGAAAAACCAAATTGCCGTGATTTCCGAG 1101 2 811 .......................................................................................... 900 Q K K M D S L S A P T I M A R L E T L I S Query_1 1102 CaaaaaaaGATGGATAGCCTCTCTGCGCCCACTATTATGGCACGATTAGAAACACTCATTTCA 1164 2 901 ............................................................... 963 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1260574 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 973 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP90994.103 (IDP90994.43-211.P(211)..pMCSG73)
QGNQNTAMTEASSAYQKVSTALSTNSAGNVSADSIALAEKFVQSNKNNYGVLVGLELAHDFVEQNAFDKAAQQLSQALGQTKDENLLSLINLRLARVQLQLKKPDEALKTLDAVQGDGWTAMAQDVRGDALLSKGDTAGARAAYSKAIESNASQALQALLRMKLNNLSS
CAGGGTAATCAGAATACTGCCATGACGGAAGCCTCCTCTGCCTATCAGAAAGTCAGTACTGCTCTGTCAACTAACAGTGCGGGTAATGTTAGTGCTGATAGCATTGCTCTCGCTGAAAAGTTTGTTCAGAGCAATAAAAATAACTACGGCGTACTCGTCGGGTTAGAACTGGCTCATGATTTTGTTGAACAAAATGCATTCGATAAAGCGGCACAGCAACTCAGTCAAGCCTTGGGCCAGACGAAAGATGAAAATCTGTTGTCGCTGATTAATCTGCGCCTTGCGCGTGTGCAACTGCAACTGAAAAAACCCGATGAAGCATTGAAAACGCTGGATGCAGTACAAGGTGATGGCTGGACAGCGATGGCTCAAGATGTTCGTGGCGATGCATTATTAAGCAAAGGTGATACCGCAGGTGCGCGCGCTGCTTATAGTAAAGCTATCGAATCTAATGCTTCTCAGGCGCTGCAAGCATTGTTGCGTATGAAACTGAATAACTTGTCCAGC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 1,793 total letters Query= IDP90994.103 Length=912 Score E Sequences producing significant alignments: (Bits) Value 04G_G10_IDP90994.103_v73F.seq 1011 0.0 wt 915 0.0 08G_G10_IDP90994.103_T7term.seq 737 0.0 W S H P Q F E K G T E N L Y F Q S N A Q G N Q N T A M T E A Query_1 145 TGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAGGGTAATCAGAATACTGCCATGACGGAAGCC 234 0 43 ...........A........G....------....................T...................................... 126 2 1 ................................. 33 S S A Y Q K V S T A L S T N S A G N V S A D S I A L A E K F Query_1 235 TCCTCTGCCTATCAGAAAGTCAGTACTGCTCTGTCAACTAACAGTGCGGGTAATGTTAGTGCTGATAGCATTGCTCTCGCTGAAAAGTTT 324 0 127 .......................................................................................... 216 2 34 .......................................................................................... 123 1 486 .......NN................N.......... 451 V Q S N K N N Y G V L V G L E L A H D F V E Q N A F D K A A Query_1 325 GTTCAGAGCAATAAAAATAACTACGGCGTACTCGTCGGGTTAGAACTGGCTCATGATTTTGTTGAACAAAATGCATTCGATAAAGCGGCA 414 0 217 .......................................................................................... 306 2 124 .......................................................................................... 213 1 450 ....................N.....N.....N......................................N....NN............ 361 Q Q L S Q A L G Q T K D E N L L S L I N L R L A R V Q L Q L Query_1 415 CAGCAACTCAGTCAAGCCTTGGGCCAGACGAAAGATGAAAATCTGTTGTCGCTGATTAATCTGCGCCTTGCGCGTGTGCAACTGCAACTG 504 0 307 .......................................................................................... 396 2 214 .......................................................................................... 303 1 360 .................N..........N...............................N............................. 271 K K P D E A L K T L D A V Q G D G W T A M A Q D V R G D A L Query_1 505 AAAAAACCCGATGAAGCATTGAAAACGCTGGATGCAGTACAAGGTGATGGCTGGACAGCGATGGCTCAAGATGTTCGTGGCGATGCATTA 594 0 397 .......................................................................................... 486 2 304 .......................................................................................... 393 1 270 .......................................................................................... 181 L S K G D T A G A R A A Y S K A I E S N A S Q A L Q A L L R Query_1 595 TTAAGCAAAGGTGATACCGCAGGTGCGCGCGCTGCTTATAGTAAAGCTATCGAATCTAATGCTTCTCAGGCGCTGCAAGCATTGTTGCGT 684 0 487 .......................................................................................... 576 2 394 .......................................................................................... 483 1 180 ....................................N...........................N......................... 91 M K L N N L S S * H W K W I T D P N S S S V D K L A A A L E Query_1 685 ATGAAACTGAATAACTTGTCCAGCTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAG 774 0 577 .......................N....................--..... 625 2 484 ........................ 507 1 90 T.....N.....................................--..... 42 H H H H H H * D P A A N K A R K E A E L A A A T A E Q * L Query_1 775 caccaccaccaccaccacTGAGATCCGGCTGCTAAC-AAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGC 863 0 621 ..............-.N.............N.................N.............N..... 687 1 46 ..............A......N.... 21 A * P L G A S K R V L R G F L L Query_1 864 ATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGA 910 0 688 ............................................... 734 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1567956 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 1,793 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP91062.103 (IDP91062.26-266.P(266)..pMCSG73)
ADATSPVVTNSAFKNNDQQSAYALGASLGRYMDNSLKEQEKLGIKLDKDQLIAGVQDAFANKSKLTDEEIEKTLQNFEARVKASAQAKMEQDAKENADKGAKYRDTFAKEKGVKKTASGLLYKVENAGTGDAPKDSDTVVVNYKGTLADGTEFDNSYKRGEPLSFRLDGVIPGWTEGLKQIKKGGKITLVIPPELAYGKAGVPGIPANSTLIFDVELLDVKAAAKADAQEQQPAVDTKAKK
GCTGACGCAACCTCACCAGTAGTAACCAATAGCGCATTTAAAAATAATGATCAGCAATCTGCTTATGCATTAGGTGCGTCTTTGGGCCGCTATATGGATAATTCCCTGAAAGAACAAGAAAAATTGGGGATTAAGTTGGATAAAGACCAGTTGATCGCTGGTGTGCAAGATGCTTTTGCTAACAAAAGCAAACTGACTGACGAAGAGATCGAAAAAACCCTGCAAAATTTTGAAGCTCGGGTAAAAGCGTCTGCTCAAGCCAAAATGGAGCAAGATGCCAAAGAAAACGCGGATAAAGGCGCTAAGTACCGCGATACCTTTGCTAAAGAAAAAGGTGTGAAGAAAACCGCATCTGGCCTGCTGTACAAGGTAGAGAACGCCGGTACGGGTGATGCACCAAAAGACAGCGATACTGTTGTCGTTAACTACAAAGGCACTTTGGCTGACGGAACTGAGTTTGATAACTCATACAAACGTGGCGAGCCATTGTCTTTCCGTCTGGATGGCGTTATCCCAGGCTGGACTGAAGGCCTGAAGCAAATCAAAAAAGGTGGCAAAATCACCTTGGTTATCCCGCCAGAACTGGCTTACGGCAAAGCAGGCGTCCCTGGTATTCCTGCCAACTCTACTCTGATATTCGATGTAGAACTGCTGGATGTGAAAGCCGCAGCTAAAGCCGATGCTCAAGAGCAACAGCCTGCGGTTGATACAAAAGCTAAGAAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 1,089 total letters Query= IDP91062.103 Length=1128 Score E Sequences producing significant alignments: (Bits) Value wt 1305 0.0 08B_B10_IDP91062.103_T7term.seq 477 2e-138 A D A T S P V V T N S A F K N N D Q Q S A Y A L G A S L G R Query_1 202 GCTGACGCAACCTCACCAGTAGTAACCAATAGCGCATTTAAAAATAATGATCAGCAATCTGCTTATGCATTAGGTGCGTCTTTGGGCCGC 291 2 1 .......................................................................................... 90 Y M D N S L K E Q E K L G I K L D K D Q L I A G V Q D A F A Query_1 292 TATATGGATAATTCCCTGAAAGAACAAGAAAAATTGGGGATTAAGTTGGATAAAGACCAGTTGATCGCTGGTGTGCAAGATGCTTTTGCT 381 2 91 .......................................................................................... 180 N K S K L T D E E I E K T L Q N F E A R V K A S A Q A K M E Query_1 382 AACAAAAGCAAACTGACTGACGAAGAGATCGAAAAAACCCTGCAAAATTTTGAAGCTCGGGTAAAAGCGTCTGCTCAAGCCAAAATGGAG 471 2 181 .......................................................................................... 270 Q D A K E N A D K G A K Y R D T F A K E K G V K K T A S G L Query_1 472 CAAGATGCCAAAGAAAACGCGGATAAAGGCGCTAAGTACCGCGATACCTTTGCTAAAGAAAAAGGTGTGAAGAAAACCGCATCTGGCCTG 561 2 271 .......................................................................................... 360 L Y K V E N A G T G D A P K D S D T V V V N Y K G T L A D G Query_1 562 CTGTACAAGGTAGAGAACGCCGGTACGGGTGATGCACCAAAAGACAGCGATACTGTTGTCGTTAACTACAAAGGCACTTTGGCTGACGGA 651 2 361 .......................................................................................... 450 1 361 ...N..................N...... 333 T E F D N S Y K R G E P L S F R L D G V I P G W T E G L K Q Query_1 652 ACTGAGTTTGATAACTCATACAAACGTGGCGAGCCATTGTCTTTCCGTCTGGATGGCGTTATCCCAGGCTGGACTGAAGGCCTGAAGCAA 741 2 451 .......................................................................................... 540 1 332 N....................................A.......A..N..............N...A.N.................... 243 I K K G G K I T L V I P P E L A Y G K A G V P G I P A N S T Query_1 742 ATCAAAAAAGGTGGCAAAATCACCTTGGTTATCCCGCCAGAACTGGCTTACGGCAAAGCAGGCGTCCCTGGTATTCCTGCCAACTCTACT 831 2 541 .......................................................................................... 630 1 242 .....................G......................................................N........N.... 153 L I F D V E L L D V K A A A K A D A Q E Q Q P A V D T K A K Query_1 832 CTGATATTCGATGTAGAACTGCTGGATGTGAAAGCCGCAGCTAAAGCCGATGCTCAAGAGCAACAGCCTGCGGTTGATACAAAAGCTAAG 921 2 631 .......................................................................................... 720 1 152 A...................N..........................N.................A..CC.T...-.........N.... 64 K * H W K W I T D P Query_1 922 AAGTAACATTGGAAGTGGATAACGGATCCG 951 2 721 ... 723 1 63 ........-......-.......--..... 38 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1166358 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 1,089 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP91528.103 (IDP91528.24-261.P(261)..pMCSG73)
ADTASASLSDAQKKEIEKVIHDYLINNPEVLLEASQALQQKQQQNMQQQAQAAIQENAEQVFQGKLTTVGNPKGNVTLVEFFDYQCIHCKKMASTIENLVKKDSGLRVIYKEFPIFGKTSDLASRVALAAGMQGKYQAMHNALITIDKRLDEKTVMDAAKSIGLDMQKLKKDMDSQEVTDILDANRQLAEKLHLMGTPAFIIGSTPDGQYKKGSEISFIPGATSEQSLRELIKKAAGN
GCTGATACGGCTAGCGCTTCTTTATCAGATGCTCAAAAAAAGGAAATTGAAAAAGTCATTCATGATTATCTGATTAATAACCCGGAAGTTTTATTGGAGGCTTCGCAAGCATTGCAACAAAAACAGCAACAAAATATGCAACAGCAAGCTCAAGCCGCAATTCAGGAAAATGCTGAACAAGTTTTTCAAGGCAAATTAACTACTGTTGGTAATCCTAAAGGGAATGTAACACTGGTTGAGTTTTTTGATTATCAATGCATTCATTGCAAAAAAATGGCATCTACCATTGAAAATCTGGTTAAAAAAGACAGCGGATTACGAGTTATATACAAAGAATTTCCAATCTTTGGCAAGACTTCTGATCTGGCTTCCAGAGTTGCTCTGGCGGCTGGCATGCAAGGCAAATATCAGGCTATGCATAATGCTTTGATTACGATAGACAAACGTTTGGATGAAAAAACAGTGATGGATGCCGCAAAATCCATAGGTTTGGATATGCAAAAGCTGAAAAAAGATATGGATAGTCAGGAAGTGACCGATATCCTGGATGCGAATCGCCAATTGGCTGAAAAATTACATTTGATGGGTACACCAGCCTTTATAATTGGCTCAACACCAGATGGTCAATATAAAAAGGGCAGTGAAATATCATTTATACCAGGCGCTACAAGCGAGCAATCACTACGTGAGTTAATTAAAAAGGCGGCTGGCAAT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,404 total letters Query= IDP91528.103 Length=1119 Score E Sequences producing significant alignments: (Bits) Value wt 1288 0.0 07D_D9_IDP91528.103_T7term.seq 1283 0.0 03D_D9_IDP91528.103_v73F.seq 1196 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAA- 197 1 834 ....T......A........G....AG.-----................N...A 786 0 45 ....T......A........G....------......................- 91 0 27 .....C........... 43 N A A D T A S A S L S D A Q K K E I E K V I H D Y L I N N Query_1 198 TGCCGCTGATACGGCTAGCGCTTCTTTATCAGA-TGCTCaaaaaaaGGAAA-TTGAAAAA--GTCATTCATGATTATCTGATTAATAACC 283 2 1 .............................-.................-........--............................ 82 1 785 .................................A...C.............N........AA...............N............ 696 0 92 .................................-...C.............-........--............................ 177 P E V L L E A S Q A L Q Q K Q Q Q N M Q Q Q A Q A A I Q E N Query_1 284 CGGAAGTTTTATTGGAGG-CTTCGCAAGCATTGCAACAAAAACAGCAACAAAATATGCAACAGCAAGCTCAAGCCGCAATTCAGGAAAAT 372 2 83 ..................-....................................................................... 171 1 695 ..................G..A..........A..............G..........................T............... 606 0 178 ..................-.............A..............G..........................T............... 266 A E Q V F Q G K L T T V G N P K G N V T L V E F F D Y Q C I Query_1 373 GCTGAACAAGTTTTTCAAGGCAAATTAACTACTGTTGGTAATCCTAAAGGGAATGTAACACTGGTTGAGTTTTTTGATTATCAATGCATT 462 2 172 .......................................................................................... 261 1 605 ........G..........................A...................................................... 516 0 267 ........G..........................A...................................................... 356 H C K K M A S T I E N L V K K D S G L R V I Y K E F P I F G Query_1 463 CATTGCaaaaaaaTGGCATCTACCATTGAAAATCTGGTTAAAAAAGACAGCGGATTACGAGTTATATACAAAGAATTTCCAATCTTTGGC 552 2 262 .......................................................................................... 351 1 515 .......................................................................................... 426 0 357 .......................................................................................... 446 K T S D L A S R V A L A A G M Q G K Y Q A M H N A L I T I D Query_1 553 AAGACTTCTGATCTGGCTTCCAGAGTTGCTCTGGCGGCTGGCATGCAAGGCAAATATCAGGCTATGCATAATGCTTTGATTACGATAGAC 642 2 352 .......................................................................................... 441 2 702 ........... 712 1 425 ..................................................A....................................... 336 1 75 ........... 65 0 447 ..................................................A....................................... 536 K R L D E K T V M D A A K S I G L D M Q K L K K D M D S Q E Query_1 643 AAACGTTTGGATGAAAAAACAGTGATGGATGCCGCAAAATCCATAGGTTTGGATATGCAAAAGCTGAAAAAAGATATGGATAGTCAGGAA 732 2 442 .......................................................................................... 531 1 335 ...................................C............C.............A........................... 246 0 537 ...................................C............C.............A........................... 626 V T D I L D A N R Q L A E K L H L M G T P A F I I G S T P D Query_1 733 GTGACCGATATCCTGGATGCGAATCGCCAATTGGCTGAAAAATTACATTTGATGGGTACACCAGCCTTTATAATTGGCTCAACACCAGAT 822 2 532 .......................................................................................... 621 1 245 ..............................C........................................................... 156 0 627 ..............................C..................................................N........ 716 G Q Y K K G S E I S F I P G A T S E Q S L R E L I K K A A Query_1 823 -GGTCAATATAAAAAGGGCAGTGAAATATCATTTATA-CCAGGCGCTACAAGCGAGCAATCACTACGTGAGTTAATTAAAAAGGCGGCTG 910 2 622 -....................................-.................................................... 709 2 384 ........ 391 1 155 -..G.................................-.................................................... 68 1 393 ........ 386 0 717 G..N.................................C.......... 764 0 479 ........ 486 G N * H W K W I T D P N S S S V D K L A A A L E H H H H H H Query_1 911 GCAATTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacT 1000 2 710 ..... 714 2 392 ... 394 1 67 .........................--..... 38 1 385 ... 383 0 487 ... 489 * D P A A N K A R K E Query_1 1001 GAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGC 1034 1 42 ............................... 12 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2604336 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 2,404 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95771.107 (IDP95771.19-382.P(628)..pMCSG73)
AKKLAKKYKKDKDVNITLIDRHSYHTMMTELHEVAGGRVEPTAIQYDLRRLFNRTKVNLVTDNVTHVDHDKKVVTTEHGSYPFDYLVLGMGGEPNDFGTPGVGENGFTLWSWEDSVKLRNHIEETVTKASREQDVEKRKAMLTFVVCGSGFTGIEMVGELLEWKDRLAKDNKIDASEIKLVVVEAAPTILNMLERRDADKAERYMVKKGIEIMKNAAIVEVKPESIVLKSGEELPTSTLIWTAGVRANSDTKDYGMESARAGRLKVNQYMEAEGLKDVYVVGDLAYFEDEEGKPTPQIVEGAEQTALTAAKSIIVEMSGTGEKEPFQGKYHGVMVSIGAKYGVAHLGGMHLSGWFAILMKHMVN
GCTAAGAAATTAGCTAAGAAATACAAGAAAGACAAAGACGTTAATATTACATTAATCGATCGTCATTCGTACCATACAATGATGACTGAACTACATGAGGTTGCTGGTGGTCGTGTTGAACCAACTGCAATTCAATATGATTTACGTCGTTTGTTTAATAGAACAAAAGTTAATCTTGTAACTGATAACGTGACACATGTAGATCATGATAAAAAAGTTGTAACAACAGAGCACGGTAGTTATCCGTTTGATTACCTAGTACTTGGTATGGGCGGAGAACCTAATGATTTCGGAACTCCTGGTGTTGGCGAAAATGGCTTTACACTTTGGTCTTGGGAAGATTCTGTTAAGTTACGCAATCATATTGAAGAAACTGTAACGAAAGCATCTCGCGAACAAGACGTTGAAAAACGTAAAGCGATGTTAACATTCGTTGTTTGTGGATCTGGATTTACTGGTATCGAAATGGTTGGGGAACTTTTAGAATGGAAAGATCGTCTTGCTAAAGATAACAAAATTGACGCATCTGAAATTAAACTAGTTGTAGTAGAAGCTGCTCCAACAATCCTAAACATGCTTGAAAGAAGAGACGCTGACAAAGCAGAACGTTACATGGTTAAAAAAGGTATTGAAATCATGAAAAATGCTGCTATCGTTGAAGTTAAACCTGAAAGCATCGTACTTAAATCTGGCGAAGAACTTCCAACAAGCACATTAATTTGGACTGCTGGTGTTCGCGCTAACTCCGATACAAAAGATTACGGTATGGAATCTGCTCGTGCAGGCCGTTTGAAAGTAAACCAATATATGGAAGCAGAAGGTCTTAAAGACGTTTATGTTGTTGGTGACCTTGCTTACTTTGAAGATGAAGAAGGCAAACCAACTCCGCAAATCGTTGAAGGTGCTGAACAAACTGCACTAACTGCAGCGAAAAGTATCATCGTTGAAATGAGCGGTACTGGCGAGAAAGAACCATTCCAAGGTAAATACCATGGTGTAATGGTTTCTATTGGAGCTAAATATGGTGTTGCTCATCTTGGTGGCATGCATCTTTCTGGTTGGTTCGCTATTTTAATGAAACATATGGTTAAC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,767 total letters Query= IDP95771.107 Length=1497 Score E Sequences producing significant alignments: (Bits) Value wt 1970 0.0 03E_E9_IDP95771.107_v73F.seq 1436 0.0 07E_E9_IDP95771.107_T7term.seq 1128 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 0 41 ...........A........G....------................-.....G 87 0 25 ...C........... 39 0 26 .............. 39 A A K K L A K K Y K K D K D V N I T L I D R H S Y H T M M T Query_1 199 GCCGCTAAGAAATTAGCTAAGAAATACAAGAAAGACAAAGACGTTAATATTACATTAATCGATCGTCATTCGTACCATACAATGATGACT 288 2 1 ....................................................................................... 87 0 88 ......................................N...N............................................... 177 E L H E V A G G R V E P T A I Q Y D L R R L F N R T K V N L Query_1 289 GAACTACATGAGGTTGCTGGTGGTCGTGTTGAACCAACTGCAATTCAATATGATTTACGTCGTTTGTTTAATAGAACAAAAGTTAATCTT 378 2 88 .......................................................................................... 177 2 369 ............ 358 0 178 .......................................................................................... 267 0 459 ........... 449 V T D N V T H V D H D K K V V T T E H G S Y P F D Y L V L G Query_1 379 GTAACTGATAACGTGACACATGTAGATCATGATAAAAAAGTTGTAACAACAGAGCACGGTAGTTATCCGTTTGATTACCTAGTACTTGGT 468 2 178 .......................................................................................... 267 0 268 .......................................................................................... 357 M G G E P N D F G T P G V G E N G F T L W S W E D S V K L R Query_1 469 ATGGGCGGAGAACCTAATGATTTCGGAACTCCTGGTGTTGGCGAAAATGGCTTTACACTTTGGTCTTGGGAAGATTCTGTTAAGTTACGC 558 2 268 .......................................................................................... 357 0 358 .......................................................................................... 447 N H I E E T V T K A S R E Q D V E K R K A M L T F V V C G S Query_1 559 AATCATATTGAAGAAACTGTAACGAAAGCATCTCGCGAACAAGACGTTGAAAAACGTAAAGCGATGTTAACATTCGTTGTTTGTGGATCT 648 2 358 .......................................................................................... 447 2 142 ............ 131 0 448 N.........................N........N...................................................... 537 0 232 ............ 221 G F T G I E M V G E L L E W K D R L A K D N K I D A S E I Query_1 649 GGATTTACTGGTATCGAAATGGTTGGGGAACTTTTAGAATGGAAAGATCGTCTTGCTAAAGATAACAAAATTGACGCATCTGAAA-TTAA 737 2 448 .....................................................................................-.... 536 0 538 .....................................................................................T.... 627 1 681 ...............................................N..........-.... 620 K L V V V E A A P T I L N M L E R R D A D K A E R Y M V K K Query_1 738 ACTAGTTGTAGTAGAAGCTGCTCCAACAATCCTAAACATGCTTGAAAGAAGAGACGCTGACAAAGCAGAACG-TTACATGGTTAAAAAAG 826 2 537 ........................................................................-................. 625 0 628 ........................-......................N.....................N..T................. 716 1 619 ........................................................................-................. 531 G I E I M K N A A I V E V K P E S I V L K S G E E L P T S Query_1 827 GTATTGAAATCATGAAAAATGCTGCTATCGTTGAAGTTAAA-CCTGAAAGCATCGTAC-TTAAATCTGGCGAAGAACTTCCAACAAGCAC 914 2 626 .........................................-................-............................... 713 0 717 ..........................T..N.NA.....N..T................T....T......N.T..CT........N.... 806 1 530 .........................................-................-............................... 443 T L I W T A G V R A N S D T K D Y G M E S A R A G R L K V N Query_1 915 ATTAATTTGGACTGCTGGTGTTCGCGCTAACT-CCGATACAAAAGATTACGGTATGGAA-TCTGCTCGTGCAGGCCGTTTGAAAGTAAAC 1002 2 714 ................................-..........................-.............................. 801 0 807 ..N....................N........N..............A....-......N........N........-........N... 894 1 442 ................................-..........................-....N....N.................... 355 Q Y M E A E G L K D V Y V V G D L A Y F E D E E G K P T P Query_1 1003 CAATATA-TGGAAGCAGAAGGTCTTAAAGACGTTTATGTTGTTGGTGACCTTGCTTACTTTGAAGATGAAGAAGGCAAACCAACTCCGCA 1091 2 802 .......-.................................................................................. 890 0 895 ..T....T..T....T..T......-..N.........-........................N.N..-.N....TN...NNCT...... 981 1 354 .......-.................................................................................. 266 Q I V E G A E Q T A L T A A K S I I V E M S G T G E K E P F Query_1 1092 AATCGTTGAAGGTGCTGAACAAACTGCACTAACTGCAGCGAAAAGTATCATCGTTGAAATGAGCGGTACTGGCGAGAAAGAACCATTCCA 1181 2 891 .......................................................................................... 980 0 982 ..CN......... 994 1 265 ................................N......................................................... 176 Q G K Y H G V M V S I G A K Y G V A H L G G M H L S G W F A Query_1 1182 AGGTAAATACCATGGTGTAATGGTTTCTATTGGAGCTAAATATGGTGTTGCTCATCTTGGTGGCATGCATCTTTCTGGTTGGTTCGCTAT 1271 2 981 .......................................................................................... 1070 1 175 .........................................................................................A 86 I L M K H M V N * H W K W I T D P N S S S V D K L A A A L E Query_1 1272 TTTAATGAAACATATGGTTAACTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGca 1361 2 1071 ...................... 1092 1 85 .....G....................................--..... 39 H H H H H H * D P A A N K A R K E Query_1 1362 ccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCG-AAAGGAA 1410 1 43 ......................A....... 14 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4026839 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 2,767 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95802.103 (IDP95802.1-354.F..pMCSG73)
MLSPNVDTALNMLTDVYTDFLGISNYDATCNSLFIGHVAKDPNWLVEVRSRTEILRAVMNEFMQQKPKIFAQIITSFINYQTTFDACAQNSKAITSTKQWIECLQLLQKTLKQNITLTNEAQQVFTKSYNQAKNAEELLASSIQDGWNELASEEQAMVRIATEIGSLSQSIASLGANVTAAQLRAGKAYIQSMVTISYGVVMGATTSVPFLSFAGALFTVGYSAYSTISSAKEVQQDLDKLTQLQTLASEEAQAAAITKAIIQTLSNMSEEFLKIDDSLPALSLLWQDELDKVNELINALQSGSDPALLTDLQTIKIASASWKTISEFVQLISLPPNVGKPVLVNTLNNTIQEQ
ATGTTAAGCCCAAATGTTGATACCGCATTAAATATGCTGACCGACGTATATACTGATTTTTTAGGTATTAGTAATTACGATGCAACGTGCAACTCTCTCTTTATTGGCCATGTAGCAAAAGATCCAAACTGGCTCGTAGAAGTTCGAAGCAGGACTGAGATCTTAAGAGCGGTAATGAATGAGTTTATGCAGCAAAAGCCTAAAATTTTTGCGCAAATTATTACATCGTTTATTAATTATCAGACCACGTTCGATGCCTGCGCACAAAACAGTAAGGCGATAACAAGTACGAAGCAATGGATTGAATGCTTACAATTATTGCAAAAAACGTTAAAGCAAAATATCACGTTAACAAATGAAGCTCAGCAAGTTTTCACGAAATCTTACAACCAAGCGAAAAATGCAGAGGAGCTACTCGCATCCAGCATTCAAGATGGCTGGAATGAACTTGCGAGCGAAGAACAAGCAATGGTACGCATTGCAACAGAGATAGGCTCTCTATCGCAATCCATTGCCAGTTTAGGAGCCAATGTCACGGCGGCCCAATTAAGAGCAGGAAAAGCTTACATACAAAGTATGGTCACTATTTCCTACGGTGTCGTAATGGGAGCAACGACGTCTGTGCCATTCCTTTCCTTTGCTGGTGCGCTATTCACGGTTGGTTACAGTGCATACAGCACAATATCGAGCGCGAAAGAGGTACAACAAGATTTAGACAAACTCACTCAATTACAAACACTAGCCAGTGAAGAAGCACAAGCGGCTGCCATTACTAAAGCCATTATTCAAACCCTCAGCAACATGAGCGAAGAATTCCTAAAGATTGATGATAGCTTGCCAGCACTTTCATTGTTATGGCAAGACGAATTAGACAAGGTAAACGAACTGATCAACGCCTTACAAAGCGGTTCAGACCCTGCCCTATTGACTGATTTGCAAACCATCAAGATTGCTTCAGCCTCATGGAAAACCATTTCAGAATTTGTGCAACTCATTAGTTTACCGCCAAACGTTGGAAAACCCGTTTTAGTCAACACACTAAATAACACCATTCAAGAACAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,982 total letters Query= IDP95802.103 Length=1467 Score E Sequences producing significant alignments: (Bits) Value wt 1916 0.0 08H_H10_IDP95802.103_T7term.seq 1817 0.0 04H_H10_IDP95802.103_v73F.seq 1285 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 0 40 ...........A........G....------.................C.T... 87 0 24 ...C........... 38 0 25 .............. 38 A M L S P N V D T A L N M L T D V Y T D F L G I S N Y D A Query_1 199 GCCATGTTAAGCCCAAATGTTGATACCGCATTAAATAT-GCTGACC-GACGTATATACTGATTTTTTAGG-TATTAGTAATTACGATGCA 285 2 1 ...................................-.......-.......................-................... 84 1 1116 ........N....N.N...N.......N.......C.......N..........--...AN..N............... 1040 0 88 ......................................-.......-.......................-................... 174 T C N S L F I G H V A K D P N W L V E V R S R T E I L R A Query_1 286 ACG-TGCAACTCTCTCTTTATTGGCCATGTAGCAAAAGAT-CCAAACTGGCTCGTAGAAGTTCGAAGCAGGACTGAGATCTTAAGAGCGG 373 2 85 ...-....................................-................................................. 172 1 1039 ...G.............A...-..........NC......T....G.................................N.......... 951 0 175 ...-....................................-................................................. 262 V M N E F M Q Q K P K I F A Q I I T S F I N Y Q T T F D A C Query_1 374 TAATGAATGAGTTTATGCAGCAAAAGCCTAAAATTTTTGCGCAAATTATTACATCGTTTATTAATTATCAGACCACGTTCGATGCCTGCG 463 2 173 .......................................................................................... 262 1 950 .........................-................................-............................... 863 0 263 .......................................................................................... 352 A Q N S K A I T S T K Q W I E C L Q L L Q K T L K Q N I T L Query_1 464 CACAAAACAGTAAGGCGATAACAAGTACGAAGCAATGGATTGAATGCTTACAATTATTGCAAAAAACGTTAAAGCAAAATATCACGTTAA 553 2 263 .......................................................................................... 352 2 515 ........... 505 1 862 ........................................-................................................. 774 1 611 ........... 621 0 353 .......................................................................................... 442 T N E A Q Q V F T K S Y N Q A K N A E E L L A S S I Q D G W Query_1 554 CAAATGAAGCTCAGCAAGTTTTCACGAAATCTTACAACCAAGCGAAAAATGCAGAGGAGCTACTCGCATCCAGCATTCAAGATGGCTGGA 643 2 353 .......................................................................................... 442 1 773 .......................................................................................... 684 0 443 .......................................................................................... 532 N E L A S E E Q A M V R I A T E I G S L S Q S I A S L G A N Query_1 644 ATGAACTTGCGAGCGAAGAACAAGCAATGGTACGCATTGCAACAGAGATAGGCTCTCTATCGCAATCCA-TTGCCAGTTTAGGAGCCAAT 732 2 443 .....................................................................-.................... 531 2 304 .......-.... 294 1 683 .....................................................................-.................... 595 0 533 .....................................................................T.................... 622 0 394 .......-.... 384 V T A A Q L R A G K A Y I Q S M V T I S Y G V V M G A T T Query_1 733 GTCACGGCGGCCCAA-TTAAGAGCAGGAAAAGCTTACATACAAAGTA-TGGTCACTATTTCCTACGGTGTCGTAATGGGAGCAACGACGT 820 2 532 ...............-...............................-.......................................... 619 1 594 ...............-...............................-.......................................... 507 0 623 ...............T.........N.....................T........................-N................ 711 S V P F L S F A G A L F T V G Y S A Y S T I S S A K E V Q Q Query_1 821 CTGTGCCATTCCTTTCCTTTGCTGGTGCGCTATTCACGGTTGGTTACAGTGCATACAGCACAATATCGAGCGCGAAAGAGGTACAACAAG 910 2 620 .......................................................................................... 709 1 506 .......................................................................................... 417 0 712 ...............................................N...............................N.G.... 797 D L D K L T Q L Q T L A S E E A Q A A A I T K A I I Q T L S Query_1 911 ATTTAGACAAACTCACTCAATTACAAACACTAGCCAGTGAAGAAGCACAAGCGGCTGCCATTACTAAAGCCATTATTCAAACCCTCAGCA 1000 2 710 .......................................................................................... 799 1 416 .......................................................................................... 327 N M S E E F L K I D D S L P A L S L L W Q D E L D K V N E L Query_1 1001 ACATGAGCGAAGAATTCCTAAAGATTGATGATAGCTTGCCAGCACTTTCATTGTTATGGCAAGACGAATTAGACAAGGTAAACGAACTGA 1090 2 800 .......................................................................................... 889 1 326 .......................................................................................... 237 I N A L Q S G S D P A L L T D L Q T I K I A S A S W K T I S Query_1 1091 TCAACGCCTTACAAAGCGGTTCAGACCCTGCCCTATTGACTGATTTGCAAACCATCAAGATTGCTTCAGCCTCATGGAAAACCATTTCAG 1180 2 890 .......................................................................................... 979 1 236 .......................................................................................... 147 E F V Q L I S L P P N V G K P V L V N T L N N T I Q E Q * H Query_1 1181 AATTTGTGCAACTCATTAGTTTACCGCCAAACGTTGGAAAACCCGTTTTAGTCAACACACTAAATAACACCATTCAAGAACAGTAACATT 1270 2 980 ................................................................................... 1062 1 146 .......................................................................................... 57 W K W I T D P N S S S V D K L A A A L E H H H H H H * D P A Query_1 1271 GGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTG 1360 1 56 .............--..... 39 1 43 ......... 35 A N K A R K E Query_1 1361 CTAAC-AAAGCCCGAAAGGAAG 1381 1 34 .....A............A... 13 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4257234 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 2,982 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95803.103 (IDP95803.1-353.F..pMCSG73)
MNQSASEQLQTDIPASISAMVLLNSACQGVVETYIDQGNAEHWYAQVEQNLNAVQKLVRQWRLSGNLYFSNDIMDSVLSIANTFKDSNVQILTLFKALETRFDTAQLQQLTSLILTLQNPIQSLTSNIKRYDEGLNAWARQVEDAHNTLQQTIAQIQQEEVSIQAEIIATNAQIDLMKQQIAAFKTAIANAQSQRKKGIFETIFGVVLAPFTLGGSLILAGFGVSSIVEAQSEISSLQSDIQSSLNTINHDQQTLSQDQQQIASLNALLLSVDQVNNDCAAISRSLDTLQTTVLSLYNETNNVVSNLTKAQDSQAVILEQVWYQSAYNEWQDILEVASTLNNAQPQITKAQIK
ATGAATCAATCAGCCAGCGAGCAACTTCAAACCGACATTCCTGCATCTATTAGCGCTATGGTGTTACTAAATTCTGCATGCCAAGGCGTTGTTGAAACTTATATTGATCAAGGCAATGCAGAACATTGGTATGCTCAGGTAGAACAAAATTTAAATGCGGTGCAAAAACTGGTACGACAATGGCGTTTGAGTGGTAATTTATATTTTTCAAATGACATTATGGACAGTGTTCTTTCCATTGCGAATACATTCAAAGATTCTAATGTTCAAATTTTAACGTTATTCAAAGCACTAGAAACCCGTTTTGATACCGCCCAACTACAGCAGCTCACTTCATTAATCTTAACATTACAAAATCCGATTCAAAGTCTTACATCAAATATAAAACGTTATGATGAGGGATTGAATGCTTGGGCTCGCCAAGTGGAAGATGCTCATAACACATTGCAACAAACTATTGCACAGATCCAGCAAGAAGAAGTTTCAATCCAAGCAGAAATTATCGCCACCAACGCCCAAATTGATCTAATGAAACAGCAGATAGCAGCATTTAAAACCGCCATTGCGAACGCTCAATCGCAGCGTAAGAAAGGGATATTTGAAACGATTTTTGGCGTGGTGCTTGCTCCGTTTACACTGGGAGGTTCTTTGATATTGGCAGGGTTTGGTGTTAGCTCTATTGTAGAAGCACAAAGTGAAATATCTAGCCTGCAAAGCGATATCCAATCATCTTTAAATACCATCAACCACGATCAACAAACGCTCTCTCAAGATCAACAACAAATCGCGTCTCTCAATGCCCTATTGCTAAGTGTCGATCAAGTTAACAACGATTGTGCGGCGATCAGCCGATCCTTAGATACACTACAAACCACGGTGCTCAGTTTATACAATGAAACCAACAATGTCGTTTCTAACCTTACCAAAGCACAAGATTCACAAGCGGTCATTCTGGAGCAAGTTTGGTACCAATCAGCTTACAATGAGTGGCAAGATATTCTGGAGGTAGCATCAACGCTCAATAATGCACAACCTCAAATAACAAAAGCTCAAATCAAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,548 total letters Query= IDP95803.103 Length=1464 Score E Sequences producing significant alignments: (Bits) Value wt 1911 0.0 09A_A11_IDP95803.103_T7term.seq 1885 0.0 09D_A11_IDP95803.103_v73F.seq 1815 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 0 1164 ........G.....-----............N.--..N.C.. 1130 1 43 ...........A........G....------....................... 90 1 25 .....C........... 41 A M N Q S A S E Q L Q T D I P A S I S A M V L L N S A C Q Query_1 199 GCCATGAATCAATCAGCCAG-CGAGCAACTTCAAACCGACATTCCTGCATCTATTAG-CGCTATGGTG-TTACTAAATTCTGCATGCCAA 285 2 1 .................-....................................-..........-..................... 84 0 1129 .G...NG..TC.........GNNN..........N.G....................G..........G...........N....N.... 1040 1 91 ....................-....................................-..........-..................... 177 G V V E T Y I D Q G N A E H W Y A Q V E Q N L N A V Q K L Query_1 286 GGCGTTGTTGAAACTTATATTGATCAAGGCAATGCAGAACATTGGTATGCTCAGGTAGAACAAAATTTAAATGCGGTGCAAAAA-CTGGT 374 2 85 ....................................................................................-..... 173 0 1039 ..................N.-.................................................-.............A..... 952 1 178 ....................................................................................-..... 266 V R Q W R L S G N L Y F S N D I M D S V L S I A N T F K D S Query_1 375 ACGACAATGGCGTTTGAGTGGTAATTTATATTTTTCAAATGACATTATGGACAGTGTTCTTTCCATTGCGAATACATTCAAAGATTCTAA 464 2 174 .......................................................................................... 263 0 951 .......................................................................................... 862 1 267 .......................................................................................... 356 N V Q I L T L F K A L E T R F D T A Q L Q Q L T S L I L T L Query_1 465 TGTTCAAATTTTAACGTTATTCAAAGCACTAGAAACCCGTTTTGATACCGCCCAACTACAGCAGCTCACTTCATTAATCTTAACATTACA 554 2 264 .......................................................................................... 353 0 861 .......................................................................................... 772 1 357 .......................................................................................... 446 Q N P I Q S L T S N I K R Y D E G L N A W A R Q V E D A H N Query_1 555 AAATCCGATTCAAAGTCTTACATCAAATATAAAACGTTATGATGAGGGATTGAATGCTTGGGCTCGCCAAGTGGAAGATGCTCATAACAC 644 2 354 .......................................................................................... 443 0 771 .......................................................................................... 682 1 447 .......................................................................................... 536 T L Q Q T I A Q I Q Q E E V S I Q A E I I A T N A Q I D L M Query_1 645 ATTGCAACAAACTATTGCACAGATCCAGCAAGAAGAAGTTTCAATCCAAGCAGAAATTATCGCCACCAACGCCCAAATTGATCTAATGAA 734 2 444 .......................................................................................... 533 0 681 .......................................................................................... 592 1 537 .......................................................................................... 626 K Q Q I A A F K T A I A N A Q S Q R K K G I F E T I F G V V Query_1 735 ACAGCAGATAGCAGCATTTAAAACCGCCATTGCGAACGCTCAATCGCAGCGTAAGAAAGGGATATTTGAAACGAT-TTTTGGCGTGGTGC 823 2 534 ...........................................................................-.............. 622 0 591 ...........................................................................-.............. 503 1 627 ...........................................................................C.............. 716 L A P F T L G G S L I L A G F G V S S I V E A Q S E I S S L Query_1 824 TTGCTCCGTTTACACTGGGAGGTTCTTTGATATTGGCAGGGTTTGGTGTTAGCTCTATTGTAGAAGCACAAAGTGAAATATCTAGCCTGC 913 2 623 .......................................................................................... 712 0 502 .......................................................................................... 413 1 717 .....................................................................-.................... 805 Q S D I Q S S L N T I N H D Q Q T L S Q D Q Q Q I A S L N A Query_1 914 AAAGCGATATCCAATCATCTTTAAATACCATCAACCACGATCAACAAACGCTCTCTCAAGATCAACAACAAATCGCGTCTCTCAATGCCC 1003 2 713 .......................................................................................... 802 0 412 .......................................................................................... 323 1 806 .......................................................................................... 895 L L L S V D Q V N N D C A A I S R S L D T L Q T T V L S L Y Query_1 1004 TATTGCTAAGTGTCGATCAAGTTAACAACGATTGTGCGGCGATCAGCCGATCCTTAGATACACTACAAACCACGGTGCTCAGTTTATACA 1093 2 803 .......................................................................................... 892 0 322 .......................................................................................... 233 1 896 ........................................................N.........................-....... 984 N E T N N V V S N L T K A Q D S Q A V I L E Q V W Y Q S A Y Query_1 1094 ATGAAACCAACAATGTCGTTTCTAACCTTACCAAAGCACAAGATTCACAAGCGGTCATTCTGGAGCAAGTTTGGTACCAATCAGCTTACA 1183 2 893 .......................................................................................... 982 0 232 .......................................................................................... 143 1 985 ...-...........N................-......-............-..............--G........C.N....C...- 1067 N E W Q D I L E V A S T L N N A Q P Q I T K A Q I K * H W K Query_1 1184 ATGAGTGGCAAGATATTCTGGAGGTAGCATCAACGCTCAATAATGCACAACCTCAAA-TAACAAAAGCTCAAATCAAGTAACATTGGAAG 1272 2 983 .........................................................-.................... 1059 0 142 .........................................................-................................ 54 1 1068 ....-AN...GN.N.........................--N......-NN......N.................-....NN..... 1149 W I T D P N S S S V D K L A A A L E H H H H H H * D P A A N Query_1 1273 TGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAAC 1362 0 53 ........--..... 41 0 45 .............. 32 K A R K E Query_1 1363 AAAGCCCG-AAAGGAAG 1378 0 31 ........A........ 15 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5068000 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 3,548 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95810.103 (IDP95810.1-377.F..pMCSG73)
MMEFTIKRDYFITQLNDTLKAISPRTTLPILTGIKIDAKEHEVILTGSDSEISIEITIPKTVDGEDIVNISETGSVVLPGRFFVDIIKKLPGKDVKLSTNEQFQTLITSGHSEFNLSGLDPDQYPLLPQVSRDDAIQLSVKVLKNVIAQTNFAVSTSETRPVLTGVNWLIQENELICTATDSHRLAVRKLQLEDVSENKNVIIPGKALAELNKIMSDNEEDIDIFFASNQVLFKVGNVNFISRLLEGHYPDTTRLFPENYEIKLSIDNGEFYHAIDRASLLAREGGNNVIKLSTGDDVVELSSTSPEIGTVKEEVDANDVEGGSLKISFNSKYMMDALKAIDNDEVEVEFFGTMKPFILKPKGDDSVTQLILPIRTY
ATGATGGAATTCACTATTAAAAGAGATTATTTTATTACACAATTAAATGACACATTAAAAGCTATTTCACCAAGAACAACATTACCTATATTAACTGGTATCAAAATCGATGCGAAAGAACATGAAGTTATATTAACTGGTTCAGACTCTGAAATTTCAATAGAAATCACTATTCCTAAAACTGTAGATGGCGAAGATATTGTCAATATTTCAGAAACAGGCTCAGTAGTACTTCCTGGACGATTCTTTGTTGATATTATAAAAAAATTACCTGGTAAAGATGTTAAATTATCTACAAATGAACAATTCCAGACATTAATTACATCAGGTCATTCTGAATTTAATTTAAGTGGCTTAGATCCAGATCAATATCCTTTATTACCTCAAGTTTCTAGAGATGACGCAATTCAATTGTCGGTAAAAGTGCTTAAAAACGTGATTGCACAAACAAATTTTGCAGTGTCCACCTCAGAAACACGCCCAGTACTAACTGGTGTGAACTGGCTTATACAAGAAAATGAATTAATATGCACAGCGACTGACTCACACCGCTTGGCTGTAAGAAAGTTGCAGTTAGAAGATGTTTCTGAAAACAAAAATGTCATCATTCCAGGTAAGGCTTTAGCTGAATTAAATAAAATTATGTCTGACAATGAAGAAGACATTGATATCTTCTTTGCTTCAAACCAAGTTTTATTTAAAGTTGGAAATGTGAACTTTATTTCTCGATTATTAGAAGGACATTATCCTGATACAACACGTTTATTCCCTGAAAACTATGAAATTAAATTAAGTATAGACAATGGGGAGTTTTATCATGCGATTGATCGTGCCTCTTTATTAGCGCGTGAAGGTGGTAATAACGTTATTAAATTAAGTACAGGTGATGACGTTGTTGAATTGTCTTCTACATCACCAGAAATTGGTACTGTAAAAGAAGAAGTTGATGCAAACGATGTTGAAGGTGGTAGCCTGAAAATTTCATTCAACTCTAAATATATGATGGATGCTTTAAAAGCAATCGATAATGATGAGGTTGAAGTTGAATTCTTCGGTACAATGAAACCATTTATTCTAAAACCAAAAGGTGACGACTCGGTAACGCAATTAATTTTACCAATCAGAACTTAC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,397 total letters Query= IDP95810.103 Length=1536 Score E Sequences producing significant alignments: (Bits) Value wt 2040 0.0 08A_A10_IDP95810.103_T7term.seq 1835 0.0 M M E F T I K R D Y F I T Q L N D T L K A I S P R T T L P Query_1 202 ATGATGGAA-TTCACTATTAAAAGAGATTATTTTATTACACAATTAAATGAC-ACATTAAAAGCTATTTCACCAAGAACAACATTACCT- 288 2 1 .........-..........................................-....................................- 87 1 1205 .......A..NC...........NN.......TA..ANNCN....TA...CN...........NGNNN.T....N............T 1118 I L T G I K I D A K E H E V I L T G S D S E I S I E I T Query_1 289 ATATTAAC-TGGTAT-CAAAAT-CGATGCGAAAGAACATGAAGTTATATTAACTGGTTCAG-ACTCTGAAATTTCAATAGAAATCACTAT 374 2 88 ........-......-......-......................................-............................ 173 1 1117 .N...T..CN.....N......T..N........N.N....-......C.NN.........G...........-.N....G......... 1030 I P K T V D G E D I V N I S E T G S V V L P G R F F V D I Query_1 375 TCCTAAAA-CTGTAGAT-GGCGAAGATATTGTCAATATTTCAGAAACAGGCTCAGTAGTACTTCCTGGACGA-TTCTTTGTTGATATTAT 461 2 174 ........-........-......................................................-................. 260 2 592 ........... 582 1 1029 ...NN...N........T......................................................N................. 940 1 606 ........... 616 I K K L P G K D V K L S T N E Q F Q T L I T S G H S E F N L Query_1 462 aaaaaaaTTACCTGGTAAAGATGTTAAATTATCTACAAATGAACAATTCCAGACATTAA-TTACATCAGGTCATTCTGAATTTAATTTAA 550 2 261 ...........................................................-.............................. 349 1 939 ...........................................................A.............................. 850 S G L D P D Q Y P L L P Q V S R D D A I Q L S V K V L K N V Query_1 551 GTGGCTTAGATCCAGATCAA-TATCCTTTATTACCTCAAGTTTCTAGAGATGACGCAATTCAATTGTCGGTAAAAGTGCTTAAAAACGTG 639 2 350 ....................-..................................................................... 438 1 849 ....................A..................................................................... 760 I A Q T N F A V S T S E T R P V L T G V N W L I Q E N E L I Query_1 640 ATTGCACAAACAAATTTTGCAGTGTCCACCTCAGAAACACGCCCAGTACTAACTGGTGTGAACTGGCTTATACAAGAAAATGAATTAATA 729 2 439 .......................................................................................... 528 1 759 ...........G.............................................................................. 670 C T A T D S H R L A V R K L Q L E D V S E N K N V I I P G K Query_1 730 TGCACAGCGACTGACTCACACCGCTTGGCTGTAAGAAAGTTGCAGTTAGAAGATGTTTCTGAAAACAAAAATGTCATCATTCCAGGTAAG 819 2 529 .......................................................................................... 618 2 219 ........... 209 1 669 .......................................................................................... 580 1 982 ........... 992 A L A E L N K I M S D N E E D I D I F F A S N Q V L F K V G Query_1 820 GCTTTAGCTGAATTAAATAAAATTATGTCTGACAATGAAGAAGACATTGATATCTTCTTTGCTTCAAACCAAGTTTTATTTAAAGTTGGA 909 2 619 .......................................................................................... 708 1 579 .......................................................................................... 490 N V N F I S R L L E G H Y P D T T R L F P E N Y E I K L S I Query_1 910 AATGTGAACTTTATTTCTCGATTATTAGAAGGACATTATCCTGATACAACACGTTTATTCCCTGAAAACTATGAAATTAAATTAAGTATA 999 2 709 .......................................................................................... 798 2 868 .............C. 882 1 489 .......................................................................................... 400 1 330 .............C. 316 D N G E F Y H A I D R A S L L A R E G G N N V I K L S T G D Query_1 1000 GACAATGGGGAGTTTTATCATGCGATTGATCGTGCCTCTTTATTAGCGCGTGAAGGTGGTAATAACGTTATTAAATTAAGTACAGGTGAT 1089 2 799 .......................................................................................... 888 2 784 .............T.. 799 2 883 . 883 2 871 .............. 858 2 960 ........... 970 1 399 ...............................................A.......................................... 310 1 315 . 315 1 414 .............T.. 399 1 327 .............. 340 1 238 ........... 228 D V V E L S S T S P E I G T V K E E V D A N D V E G G S L K Query_1 1090 GACGTTGTTGAATTGTCTTCTACATCACCAGAAATTGGTACTGTAAAAGAAGAAGTTGATGCAAACGATGTTGAAGGTGGTAGCCTGAAA 1179 2 889 .......................................................................................... 978 2 849 ........... 859 1 309 ..............A........................................................................... 220 1 349 ........... 339 I S F N S K Y M M D A L K A I D N D E V E V E F F G T M K P Query_1 1180 ATTTCATTCAACTCTAAATATATGATGGATGCTTTAAAAGCAATCGATAATGATGAGGTTGAAGTTGAATTCTTCGGTACAATGAAACCA 1269 2 979 .......................................................................................... 1068 1 219 .......................................................................................... 130 F I L K P K G D D S V T Q L I L P I R T Y * H W K W I T D P Query_1 1270 TTTATTCTAAAACCAAAAGGTGACGACTCGGTAACGCAATTAATTTTACCAATCAGAACTTACTAACATTGGAAGTGGATAACGGATCCG 1359 2 1069 ............................................................... 1131 1 129 ...................................................................................--..... 42 N S S S V D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 1360 AATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAA 1449 1 46 .........................A.N. 18 Query_1 1450 G 1450 1 17 . 17 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3570480 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 2,397 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95831.103 (IDP95831.1-428.F..pMCSG73)
MEKDPIPQATSPLATWLSYLEHLHSKTIDLGLARVSEVAGQMDIVKPAPFVFTVAGTNGKGTTCRTLETILMAAGYKVGVYSSPHLVRYTERVRIQGAELPEAAHTASFAEIEAARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVDADVAVVTSIALDHTDWLGPDRESIGREKAGIFRAGKPAVVGEPDMPLTIAEVASEKGALLQRRGVDWRYEVEGETWSFRDTAGALSHLPLPQVPLPNAATAVAALRASGLAVDDAILRAGIRDAMLPGRFQIISDAPRVILDVAHNPHAAAYLAGRLKTLAKTGRVLAVIGMLHDKDIAGTLANLAPEVDAWYCAPLEGPRGATAEQLVEHLRCGTVYSSVAQAWRAAMADAKVEDTVLVCGSFHTVAQVMEEIDAGXNRWRVSFRTV
ATGGAAAAAGATCCTATTCCTCAGGCCACGTCGCCCCTGGCTACGTGGCTTTCTTATCTGGAACATCTGCACAGTAAAACCATCGACCTTGGGCTGGCGCGCGTCAGCGAGGTCGCCGGGCAGATGGATATCGTTAAACCGGCGCCGTTCGTTTTTACCGTTGCGGGCACCAATGGCAAAGGCACCACCTGCCGCACGCTGGAAACCATCCTGATGGCGGCCGGTTATAAAGTGGGGGTCTACAGCTCTCCGCATCTGGTGCGCTACACCGAACGCGTGCGCATTCAGGGGGCTGAACTGCCGGAGGCGGCGCATACAGCTTCGTTTGCTGAAATCGAAGCGGCGCGGGGTGACATCTCGCTGACCTATTTTGAATACGGCACGCTCTCGGCGCTGTGGCTGTTTAAGCAGGCGCAGCTGGATGTGGTGATCCTTGAGGTCGGGCTCGGCGGGCGTCTCGACGCCACCAACATCGTGGATGCCGACGTCGCGGTGGTCACCAGCATCGCCCTCGATCATACCGACTGGCTGGGCCCGGATCGCGAAAGTATCGGCCGGGAAAAAGCGGGCATCTTTCGCGCCGGTAAACCGGCGGTGGTGGGGGAGCCGGATATGCCGCTCACCATCGCGGAAGTGGCTAGCGAGAAGGGCGCGCTGCTCCAGCGCCGCGGCGTCGACTGGCGCTATGAGGTGGAGGGCGAAACGTGGTCCTTCCGCGATACGGCAGGGGCGTTAAGCCACCTGCCTCTGCCTCAGGTGCCGTTGCCGAATGCGGCGACGGCCGTGGCGGCGCTGCGCGCCAGCGGGCTTGCCGTGGATGACGCTATCCTGCGCGCAGGGATCCGCGATGCGATGCTCCCGGGACGCTTTCAGATAATCAGCGACGCCCCGCGAGTGATCCTCGATGTGGCGCACAACCCGCACGCGGCGGCCTATCTCGCCGGGCGTCTCAAAACGTTAGCGAAAACGGGACGCGTGCTGGCGGTTATTGGTATGCTGCATGATAAGGATATCGCCGGCACGTTGGCCAATCTGGCCCCGGAAGTCGATGCCTGGTACTGCGCGCCGCTGGAAGGCCCGCGCGGCGCGACGGCAGAGCAGCTGGTGGAACATTTGCGCTGCGGTACAGTCTATAGCAGCGTGGCGCAGGCGTGGCGTGCGGCCATGGCTGACGCTAAAGTGGAAGATACCGTGCTGGTGTGTGGTTCGTTTCACACCGTAGCGCAGGTTATGGAAGAGATAGACGCGGGGNAGAATCGGTGGCGAGTAAGTTTCAGAACCGTT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,034 total letters Query= IDP95831.103 Length=1689 Score E Sequences producing significant alignments: (Bits) Value wt 2313 0.0 07C_C9_IDP95831.103_T7term.seq 1451 0.0 03C_C9_IDP95831.103_v73F.seq 690 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 0 40 ...........N........G....------................-...... 86 0 24 ...C........... 38 0 25 .............. 38 A M E K D P I P Q A T S P L A T W L S Y L E H L H S K T I D Query_1 199 GCCATGGAAAAAGATCCTATTCCTCAGGCCACGTCGCCCCTGGCTACGTGGCTTTCTTATCTGGAACATCTGCACAGTAAAACCATCGAC 288 2 1 ....................................................................................... 87 0 87 .N........................................................................................ 176 L G L A R V S E V A G Q M D I V K P A P F V F T V A G T N G Query_1 289 CTTGGGCTGGCGCGCGTCAGCGAGGTCGCCGGGCAGATGGATATCGTTAAACCGGCGCCGTTCGTTTTTACCGTTGCGGGCACCAATGGC 378 2 88 .......................................................................................... 177 0 177 ..G.......N........N.........GC................C.................C.....G..C..............N 266 K G T T C R T L E T I L M A A G Y K V G V Y S S P H L Query_1 379 AAAGGCACCACC--TGCCGCAC--GCTGGAAACCATCCTGATGGCGGCCGGTTATAAAG-TGGGGGTCTACAG-CTC-TCCGCATCTGGT 461 2 178 ............--........--...................................-.............-...-............ 260 1 1181 ........CT..A...NNNG.......C.GT...N.............N..T...G....NN.AAT...G..NN..G....TN..N 1096 0 267 ............--........--..........G................C.......-.............-...-............ 349 V R Y T E R V R I Q G A E L P E A A H T A S F A E I E A Query_1 462 GCGCTA-CACCG-AACGC-GTGCGCATTCAGGG-GGCTGAACTGCCGGAGGCGGCGC-ATACAGCTTCGTTTGCTGAAATCGAAGCGG-C 545 2 261 ......-.....-.....-..............-.......................-..............................-. 344 1 1095 ..NG..A.....G....NN...GN.........A...G.........N.A...A...C....G..C....N-..G..G..-.......A. 1008 0 350 ...T..-.....-.....-..............-A..G................N..-....G.NC........G..G..T.......-. 433 A R G D I S L T Y F E Y G T L S A L W L F K Q A Q L D V V I Query_1 546 GCGGGGTGACATCTCGCTGACCTATTTTGAATACGGCACGCTCTCGGCGCTGTGGCTGTTT-AAGCAGGCGCAGCTGGATGTGGTGATCC 634 2 345 .............................................................-............................ 433 1 1007 ......C.................C..G.G-.N..-.........T.....A...T..N..N.................C.......... 920 0 434 ...N..C.................C.....G..............T..N..A...T.....-...N.......N.....CN....... 520 L E V G L G G R L D A T N I V D A D V A V V T S I A L D H T Query_1 635 TTGAGGTCGGGCTCGGCGGGCGTCT-CGACGCCACCAACATCGTGGATGCCGACGTCGCGGTGGTCACCAGCATCGCCCTCGATCATACC 723 2 434 .........................-................................................................ 522 1 919 .-....NG...........C.....A.........T..T..............T...N.CN....T...........T............ 831 D W L G P D R E S I G R E K A G I F R A G K P A V V G E P Query_1 724 GACTGGCTGGGCCCGGATCGCGAAA-GTATCGGCCGGGAAAAAGCGGGCATCTTTCGCGCCGGTAAACCGGCGGTGGTGGGGGAGCCGGA 812 2 523 .........................-................................................................ 611 1 830 ...........A.............A.............G.....C..T.....C..T.....A...........A..A........... 741 D M P L T I A E V A S E K G A L L Q R R G V D W R Y E V E G Query_1 813 TATGCCGCTCACCATCGCGGAAGTGGCTAGCGAGAAGGGCGCGCTGCTCCAGCGCCGCGGCGTCGACTGGCGCTATGAGGTGGAGGGCGA 902 2 612 .......................................................................................... 701 1 740 ........A......N..T........G..............C.........................................A..... 651 E T W S F R D T A G A L S H L P L P Q V P L P N A A T A V A Query_1 903 AACGTGGTCCTTCCGCGATACGGCAGGGGCGTTAAGCCACCTGCCTCTGCCTCAGGTGCCGTTGCCGAATGCGGCGACGGCCGTGGCGGC 992 2 702 .......................................................................................... 791 1 650 G..A.....T........CG...GC............................................C..C..C.............. 561 1 561 ..C..C.............. 580 A L R A S G L A V D D A I L R A G I R D A M L P G R F Q I I Query_1 993 GCTGCGCGCCAGCGGGCTTGCCGTGGATGACGCTATCCTGCGCGCAGGGATCCGCGATGCGATGCTCCCGGGACGCTTTCAGATAATCAG 1082 2 792 .......................................................................................... 881 2 839 ............ 828 1 560 ...............A.................A...........G...........C........G....................... 471 1 581 . 581 S D A P R V I L D V A H N P H A A A Y L A G R L K T L A K T Query_1 1083 CGACGCCCCGCGAGTGATCCTCGATGTGGCGCACAACCCGCACGCGGCGGCCTATCTCGCCGGGCGTCTCAAAACGTTAGCGAAAACGGG 1172 2 882 .......................................................................................... 971 1 470 ............G....................T.................T...................................... 381 G R V L A V I G M L H D K D I A G T L A N L A P E V D A W Y Query_1 1173 ACGCGTGCTGGCGGTTATTGGTATGCTGCATGATAAGGATATCGCCGGCACGTTGGCCAATCTGGCCCCGGAAGTCGATGCCTGGTACTG 1262 2 972 .......................................................................................... 1061 1 380 .......................................C..T...................................C........... 291 C A P L E G P R G A T A E Q L V E H L R C G T V Y S S V A Q Query_1 1263 CGCGCCGCTGGAAGGCCCGCGCGGCGCGACGGCAGAGCAGCTGGTGGAACATTTGCGCTGCGGTACAGTCTATAGCAGCGTGGCGCAGGC 1352 2 1062 .......................................................................................... 1151 1 290 ...............G........G........G.......................TG....C.......................... 201 A W R A A M A D A K V E D T V L V C G S F H T V A Q V M E E Query_1 1353 GTGGCGTGCGGCCATGGCTGACGCTAAAGTGGAAGATACCGTGCTGGTGTGTGGTTCGTTTCACACCGTAGCGCAGGTTATGGAAGAGAT 1442 2 1152 .......................................................................................... 1241 1 200 .....A............................................................G..C..A................. 111 1 775 ........... 785 I D A G # N R W R V S F R T V * H W K W I T D P N S S S V D Query_1 1443 AGACGCGGGGNAGAATCGGTGGCGAGTAAGTTTCAGAACCGTTTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAA 1532 2 1242 ........................................... 1284 1 110 ..........-................................................... 50 K L A A A L E H H H H H H * D P A A N K A R K E Query_1 1533 GCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAA--GCCCGAAAGGAA 1602 1 47 .................AGC.......A... 17 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4995578 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 3,034 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95851.105 (IDP95851.49-269.P(399)..pMCSG73)
NGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVVLQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGDQIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGNITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGL
AACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,687 total letters Query= IDP95851.105 Length=1068 Score E Sequences producing significant alignments: (Bits) Value 07B_B9_IDP95851.105_T7term.seq 1270 0.0 03B_B9_IDP95851.105_v73F.seq 1225 0.0 wt 1196 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAA-TCCAA 197 1 784 ...........A........G....------.................A..... 737 1 802 .....C........... 786 0 43 ...........A........G....------.................-..... 89 0 27 ...C........... 41 N A N G F E R E L Q N N I L G L M P Q A I L S S E H G S L N Query_1 198 TGCCAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCC 287 1 736 .......................................................................................... 647 0 90 .......................................................................................... 179 2 1 ...................................................................................... 86 P Q Q L P E T A V K L D G V N R V A P I T T G D V V L Q S A Query_1 288 GCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCG 377 1 646 .......................................................................................... 557 1 414 ............. 402 0 180 .......................................................................................... 269 0 412 ............. 424 2 87 .......................................................................................... 176 2 319 ............. 331 R S V A V G V M L G I D P A Q K D P L T P Y L V N V K Q T D Query_1 378 CAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCT 467 1 556 .......................................................................................... 467 0 270 .......................................................................................... 359 2 177 .......................................................................................... 266 L E P G K Y N V I L G E Q L A S Q L G V N R G D Q I R V M V Query_1 468 CGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACC 557 1 466 .......................................................................................... 377 1 612 ............. 600 1 374 ......... 382 0 360 .......................................................................................... 449 0 214 ............. 226 0 452 ......... 444 2 267 .......................................................................................... 356 2 121 ............. 133 2 359 ......... 351 P S A S Q F T P M G R I P S Q R L F N V I G T F A A N S E V Query_1 558 ATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGA 647 1 376 .......................................T.................................................. 287 1 383 ... 385 0 450 .......................................T.................................................. 539 0 443 ... 441 2 357 .......................................................................................... 446 2 350 ... 348 D G Y E M L V N I E D A S R L M R Y P A G N I T G W R L W L Query_1 648 TGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGA 737 1 286 ....A..................................................................................... 197 0 540 ....A.....................................................N............................... 629 2 447 .......................................................................................... 536 D E P L K V D S L S Q Q K L P E G S K W Q D W R D R K G E L Query_1 738 TGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGT-AAAGGCGAG-TTG 825 1 196 ............................................................................-.........-... 109 0 630 ............................N.............................T.N...............A.........T... 719 2 537 ............................................................................-.........-... 624 F Q A V R M E K N M M G L * H W K W I T D P N S S S V D K Query_1 826 TTCCAGGCCGTACGCATGGaaaaaaaTATGA-TGGGTTTATAAC-ATTGG-AAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAG 912 1 108 ...............................-............A.....A..........ACA..... 41 0 720 .............N.................T...N........-... 766 2 625 ...............................-........ 663 L A A A L E H H H H H H * D P A A N K A R K E Query_1 913 CTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCG-AAAGGAAG 982 1 45 ......................A........ 15 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2782026 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 2,687 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95866.103 (IDP95866.1-307.F..pMCSG73)
MSMADRDGVIWYDGELVQWRDATTHVLTHTLHYGMGVFEGVRAYDTPQGTAIFRLQAHTDRLFDSAHIMNMQIPYSRDEINEATAPPCARTTWKAPISARWCSTGSEGMGLRASGLKVHVIIASWSWGAYMGEEALQQGIKVRTSSFTRHHVNISMTRAKSNGAYINSMLALQEAISGGADEAMMLDPEGYVAEGSGENIFIIKDGVIYTPEVTACLNGITRNTILTLAAEHGFKLVEKRITRDEVYIADEAFFTGTAAEVTPIREVDGRKIGAGRRGPVTEKLQKAYFDLVSGKTEAHAEWRTLVK
ATGTCGATGGCCGATCGTGATGGCGTGATCTGGTATGACGGTGAACTGGTGCAGTGGCGCGACGCGACCACGCACGTGCTGACCCATACCCTGCACTATGGAATGGGCGTGTTCGAGGGCGTGCGCGCCTACGACACCCCGCAGGGCACGGCGATCTTCCGCCTGCAGGCGCATACCGACCGGCTGTTCGACTCCGCGCACATCATGAACATGCAGATCCCGTACAGCCGCGACGAGATCAACGAGGCGACCGCGCCGCCGTGCGCGAGAACAACCTGGAAAGCGCCTATATCCGCCCGATGGTGTTCTACGGGAAGCGAAGGCATGGGCCTGCGCGCCAGCGGCCTGAAGGTCCATGTGATCATCGCCTCCTGGAGCTGGGGCGCCTACATGGGCGAGGAAGCCCTGCAGCAAGGCATCAAGGTGCGCACCAGTTCCTTCACCCGCCACCACGTCAACATCTCGATGACCCGTGCCAAGTCCAACGGCGCCTACATCAACTCGATGCTGGCCCTCCAGGAAGCGATCTCCGGCGGCGCCGACGAGGCCATGATGCTCGATCCGGAAGGCTACGTGGCCGAAGGCTCCGGCGAGAACATCTTCATCATCAAGGATGGCGTGATCTACACCCCGGAAGTCACCGCCTGCCTGAACGGCATCACTCGTAACACTATCCTGACCCTGGCCGCCGAACACGGTTTTAAACTGGTCGAGAAGCGCATCACCCGCGACGAGGTGTACATCGCCGACGAGGCCTTCTTCACTGGCACTGCCGCGGAAGTCACGCCGATCCGCGAAGTGGACGGTCGCAAGATCGGCGCCGGCCGCCGTGGCCCGGTCACCGAAAAGCTGCAGAAAGCCTATTTCGACCTGGTCAGCGGCAAGACCGAGGCCCACGCCGAGTGGCGTACCCTGGTCAAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,522 total letters Query= IDP95866.103 Length=1326 Score E Sequences producing significant alignments: (Bits) Value wt 1662 0.0 07G_G9_IDP95866.103_T7term.seq 1389 0.0 03G_G9_IDP95866.103_v73F.seq 1162 0.0 W S H P Q F E K G T E N L Y F Q S N A M S M A D R D G V I W Query_1 145 TGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGTCGATGGCCGATCGTGATGGCGTGATCTGG 234 2 1 ................................. 33 2 613 ............. 625 1 374 ............. 362 0 43 ...........A..N.....G....------........................................................... 126 0 707 ............. 719 Y D G E L V Q W R D A T T H V L T H T L H Y G M G V F E G V Query_1 235 TATGACGGTGAACTGGTGCAGTGGCGCGACGCGACCACGCACGTGCTGACCCATACCCTGCACTATGGAATGGGCGTGTTCGAGGGCGTG 324 2 34 .......................................................................................... 123 0 127 .......................................................................................... 216 R A Y D T P Q G T A I F R L Q A H T D R L F D S A H I M N M Query_1 325 CGCGCCTACGACACCCCGCAGGGCACGGCGATCTTCCGCCTGCAGGCGCATACCGACCGGCTGTTCGACTCCGCGCACATCATGAACATG 414 2 124 .......................................................................................... 213 2 171 ............ 160 1 836 .........................................N.............N....... 774 1 816 ............ 827 0 217 ...........................................N.............................................. 306 0 264 ....N....... 253 Q I P Y S R D E I N E A T A P P C A R T T W K A P I S A R Query_1 415 CAGATCCCGTACAGCCGCGACGAGATCAACGAGGCGA-CC-GCGCCGCCGTGCGCGAGAACAACCTGGAAAGCGCCTATATCCGCCCGAT 502 2 214 .....................................-..-................................................. 301 1 773 .................N...................-..C................................................. 685 0 307 .....................N...............C..-................................................. 395 W C S T G S E G M G L R A S G L K V H V I I A S W S W G A Y Query_1 503 GGTGTTCTACGGGAAGCGAAGGCATGGGCCTGCGCGCCAGCGGCCTGAAGGTCCATGTGATCATCGCCTCCTGGAGCTGGGGCGCCTACA 592 2 302 .......................................................................................... 391 2 487 .......... 496 1 684 ............-.......................................................G..................... 596 1 500 .......... 491 0 396 ..........-.........................................................G....................N 484 0 580 .......... 589 M G E E A L Q Q G I K V R T S S F T R H H V N I S M T R A K Query_1 593 TGGGCGAGGAAGCCCTGCAGCAAGGCATCAAGGTGCGCACCAGTTCCTTCACCCGCCACCACGTCAACATCTCGATGACCCGTGCCAAGT 682 2 392 .......................................................................................... 481 2 497 . 497 1 595 ..................................................................................C....... 506 1 490 . 490 0 485 ...................N.........T.......T............................................C....... 574 0 590 . 590 S N G A Y I N S M L A L Q E A I S G G A D E A M M L D P E G Query_1 683 CCAACGGCGCCTACATCAACTCGATGCTGGCCCTCCAGGAAGCGATCTCCGGCG-GCGCCGACGAGGCCATGATGCTCGATCCGGAAGGC 771 2 482 ......................................................-................................... 570 2 744 ............. 756 2 382 ........... 392 1 505 ......................................................-................................... 417 1 243 ............. 231 1 605 ........... 595 0 575 ................N.....................................T............N...................... 664 Y V A E G S G E N I F I I K D G V I Y T P E V T A C L N G I Query_1 772 TACGTGGCCGAAGGCTCCGGCGAGAACATCTTCATCATCAAGGATGGCGTGATCTACACCCCGGAAGTCACCGCCTGCCTGAACGGCATC 861 2 571 .......................................................................................... 660 2 19 ............. 31 1 416 .......................................................................................... 327 0 665 ......................................T.............................T....T...NT...T.T.T... 754 0 112 ............. 124 T R N T I L T L A A E H G F K L V E K R I T R D E V Y I A D Query_1 862 ACTCGTAACACTATCCTGACCCTGGCCGCCGAACACGGTTTTAAACTGGTCGAGAAGCGCATCACCCGCGACGAGGTGTACATCGCCGAC 951 2 661 .......................................................................................... 750 2 537 ....... 543 1 326 .......................................................................................... 237 1 450 ....... 444 0 755 T... 758 0 631 ....... 637 E A F F T G T A A E V T P I R E V D G R K I G A G R R G P V Query_1 952 GAGGCCTTCTTCACTGGCACTGCCGCGGAAGTCACGCCGATCCGCGAAGTGGACGGTCGCAAGATCGGCGCCGGCCGCCGTGGCCCGGTC 1041 2 751 .......................................................................................... 840 2 544 ...... 549 1 236 .......................................................................................... 147 1 443 ...... 438 0 638 .... 641 T E K L Q K A Y F D L V S G K T E A H A E W R T L V K * H W Query_1 1042 ACCGAAAAGCTGCAGAAAGCCTATTTCGACCTGGTCAGCGGCAAGACCGAGGCCCACGCCGAGTGGCGTACCCTGGTCAAGTAACATTGG 1131 2 841 ................................................................................. 921 1 146 .............................................................G............................ 57 K W I T D P N S S S V D K L A A A L E H H H H H H * D P A A Query_1 1132 AAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCT 1221 1 56 ...........--..... 41 1 45 ........... 35 N K A R Query_1 1222 AACAAAGCCCGAAA 1235 1 34 .............. 21 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3240940 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 2,522 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95891.103 (IDP95891.1-286.F..pMCSG73)
MKSLAFIHRNDTRFAVGDFYPVLSVFSYHELGNTLSPFLLLDHSGPGKIAPSMKRRGVNDHPHRGFETVTLVYAGELEHKDSSGGGGFISAGDVQWMTAASGVIHRELFSEEYSRSGGPFEMIQLWVNLPAANKMNSPRYQSLKKAEIPMVKLENEAGFVRIIAGQFNHVIGPALTHTPITVLDVELHAGQQAAFPAQSGETALVYLRSGRAQFQKDEDVLEEQGLAVMSNQGEHFSITALQSCKLLILTGQPITEPINGHGPFVMNTYDEILQAYDDIKNGRFAK
ATGAAAAGTCTGGCTTTTATTCATCGTAACGATACTCGCTTTGCGGTCGGTGATTTTTATCCAGTTCTTTCTGTTTTTTCCTACCATGAGTTAGGCAATACCCTCTCACCCTTCTTACTACTCGACCATTCAGGTCCCGGAAAAATTGCACCTTCTATGAAACGTAGAGGAGTTAATGATCATCCCCATCGTGGATTCGAAACGGTAACACTTGTTTATGCAGGTGAACTAGAACACAAAGATTCATCTGGTGGAGGTGGATTTATAAGTGCTGGTGATGTGCAATGGATGACCGCAGCCTCTGGTGTGATTCATCGGGAATTGTTTAGTGAAGAGTATTCTCGATCTGGTGGTCCTTTTGAAATGATTCAACTTTGGGTCAATTTACCAGCAGCAAATAAAATGAACTCTCCGCGCTATCAAAGTTTAAAGAAAGCTGAAATACCGATGGTGAAACTGGAAAATGAGGCTGGATTTGTACGTATTATTGCAGGGCAGTTTAACCATGTTATAGGTCCTGCACTTACTCACACTCCTATTACCGTATTAGATGTAGAGCTCCATGCAGGTCAACAAGCCGCCTTCCCTGCTCAATCTGGTGAAACAGCTTTAGTTTATTTACGTTCAGGTCGGGCACAATTTCAAAAAGATGAAGACGTTTTAGAGGAACAAGGCCTAGCAGTCATGTCAAACCAAGGAGAGCATTTCTCTATTACTGCCCTGCAAAGTTGTAAATTACTTATTTTGACAGGACAACCCATCACAGAACCGATTAATGGTCATGGTCCATTTGTAATGAATACTTATGATGAGATTTTACAAGCCTATGATGATATTAAAAATGGAAGATTTGCCAAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,051 total letters Query= IDP95891.103 Length=1263 Score E Sequences producing significant alignments: (Bits) Value wt 1548 0.0 05A_A7_IDP95891.103_T7term.seq 933 0.0 01A_A7_IDP95891.103_v73F.seq 888 0.0 E N L Y F Q S N A M K S L A F I H R N D T R F A V G D F Y Query_1 175 GAGAACCT-GTACTTCCAATCCAATGCCATGAAAAGTCTGGCTTTTATTCATCGTAACGATACTCGCTTTGCGGTCGGTGATTTTTATCC 263 2 1 .............................................................. 62 0 13 ........T.........N...GTAANNN................................N..TTN....................... 102 P V L S V F S Y H E L G N T L S P F L L L D H S G P G K I A Query_1 264 AGTTCTTTCTGTTTTTTCCTACCATGAGTTAGGCAATACCCTCTCACCCTTCTTACTACTCGACCATTCAGGTCCCGGAAAAATTGCACC 353 2 63 .......................................................................................... 152 0 103 ......................................................................T................... 192 P S M K R R G V N D H P H R G F E T V T L V Y A G E L E H K Query_1 354 TTCTATGAAACGTAGAGGAGTTAATGATCATCCCCATCGTGGATTCGAAACGGTAACACTTGTTTATGCAGGTGAACTAGAACACAAAGA 443 2 153 .......................................................................................... 242 0 193 ...N..........N...............................T......................N.........N....T..... 282 D S S G G G G F I S A G D V Q W M T A A S G V I H R E L F S Query_1 444 TTCATCTGGTGGAGGTGGATTTATAAGTGCTGGTGATGTGCAATGGATGACCGCAGCCTCTGGTGTGATTCATCGGGAATTGTTTAGTGA 533 2 243 .......................................................................................... 332 1 603 ............. 591 0 283 ..........................N.............N..............N...............N....N............. 372 E E Y S R S G G P F E M I Q L W V N L P A A N K M N S P R Y Query_1 534 AGAGTATTCTCGATCTGGTGGTCCTTTTGAAATGATTCAACTTTGGGTCAATTTACCAGCAGCAAATAAAATGAACTCTCCGCGCTATCA 623 2 333 .......................................................................................... 422 1 590 ...........A............................-......................................N.......... 502 0 373 ...............................................N.............NT........................... 462 Q S L K K A E I P M V K L E N E A G F V R I I A G Q F N H V Query_1 624 AAGTTTAAAGAAAGCTGAAATACCGATGGTGAAACTGGAAAATGAGGCTGGATTTGTACGTATTATTGCAGGGCAGTTTAACCATGTTAT 713 2 423 .......................................................................................... 512 2 726 ............ 715 1 501 .......................NN.........N............NN......................................... 412 1 198 ............ 209 0 463 ..................N..........................T.............T.....N....N..T......N..N...... 552 I G P A L T H T P I T V L D V E L H A G Q Q A A F P A Q S G Query_1 714 AGGTCCTGCAC-TTACTCACACTCCTATTACCGTATTAGATGTAGAGCTCCATGCAGGTCAACAAGCCGCCTTCCCTGCTCAATCTGGTG 802 2 513 ...........-.............................................................................. 601 1 411 ...........-......G.................................N..................................... 323 1 582 ............ 571 0 553 ...........T.......................... 590 E T A L V Y L R S G R A Q F Q K D E D V L E E Q G L A V M S Query_1 803 AAACAGCTTTAGTTTATTTACGTTCAGGTCGGGCACAATTTCAAAAAGATGAAGACGTTTTAGAGGAACAAGGCCTAGCAGTCATGTCAA 892 2 602 .......................................................................................... 691 1 322 ......................................N..........N........................N............... 233 N Q G E H F S I T A L Q S C K L L I L T G Q P I T E P I N G Query_1 893 ACCAAGGAGAGCATTTCTCTATTACTGCCCTGCAAAGTTGTAAATTACTTATTTTGACAGGACAACCCATCACAGAACCGATTAATGGTC 982 2 692 .......................................................................................... 781 2 499 ............ 488 1 232 .......................................................................................... 143 1 425 ............ 436 H G P F V M N T Y D E I L Q A Y D D I K N G R F A K * H W K Query_1 983 ATGGTCCATTTGTAATGAATACTTATGATGAGATTTTACAAGCCTATGATGATATTAAAAATGGAAGATTTGCCAAGTAACATTGGAAGT 1072 2 782 ............................................................................. 858 1 142 ....................................................A..AG..........................G.A.... 53 W I T D P N S S S V D K L A A A L E H H H H H H * D P A A N Query_1 1073 GGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACA 1162 1 52 .......--..... 41 1 45 ............... 31 K A R K E Query_1 1163 AAG-CCCGA-AAGGAAG 1177 1 30 ...C.....G....... 14 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2503488 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 2,051 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95892.106 (IDP95892.1-549.F..pMCSG73)
MFKHSIFLRIYAGLVILVVLVAVFGYLLVQIINYQRAQEYRESLTDGISYVISEGVARQPGKQQKIDWISDASDLLELPIYYTDASKVELSRTEKKRIEAQKSVVRYDASNSIAYVIIGLRDDPQHYLSIKVDKITERQMKALPIFVLDYLMFYPGQEQEYLAKIQKHFSYPINIQNIQDVNLDSEQIGRLRQDQSVMLYKDSATVRGTTISIVSPIPNHPAQVLVLGPVPMFNWMPLQLSAGITLFSLFLLSLGVYGLILPLERKIRQVRYALNRMKSGDLSLRVPIEGSDEMANLASSYNNMSDHIQRLIEAQRELMRAVSHELRTPVARIRFGTEMLAEEDDYNHRMHQVDMIDKDIEALNTLIDEIMTYAKLEQGTPSLDFAEIVLFEVLDQVAVETEALKTQKEIELIPPPLYVKVDAERRYLHRVVQNLVGNAVRYCDNKVRITGGIHNDGMAFVCVEDDGPGIPEQDRKRVFEAFARLDDSRTRASGGYGLGLSIVSRIAYWFGGEIKVDESPSLGGARFIMTWPAHRFKQPPLKTNKKAPA
GTGTTTAAACACAGTATATTCCTGCGAATATATGCGGGGCTGGTAATTCTTGTTGTTTTGGTAGCTGTTTTTGGGTATTTACTGGTACAAATTATCAACTACCAACGCGCACAAGAATACCGAGAATCTTTAACTGATGGTATTTCTTATGTCATTAGTGAAGGAGTCGCTCGACAACCTGGGAAGCAGCAAAAAATAGATTGGATTTCTGACGCATCTGATTTGCTCGAACTTCCAATTTATTATACCGATGCAAGTAAGGTTGAGCTGTCTCGGACCGAGAAAAAGCGGATAGAAGCACAAAAATCTGTAGTTCGTTACGATGCAAGCAATAGCATTGCATATGTCATTATTGGCTTACGTGACGACCCACAACATTACTTATCTATAAAAGTCGACAAAATTACTGAACGCCAAATGAAGGCTCTTCCTATTTTTGTGCTCGACTATTTAATGTTTTATCCGGGGCAGGAACAAGAATATCTTGCCAAGATTCAAAAGCATTTCTCATATCCGATCAATATTCAAAATATTCAAGATGTAAATCTGGATTCTGAACAGATTGGACGTTTGCGCCAAGACCAAAGTGTCATGTTGTACAAAGATAGCGCAACAGTGCGCGGTACAACCATTTCGATTGTGTCTCCAATACCGAATCATCCTGCGCAAGTGTTAGTGCTGGGTCCGGTTCCAATGTTTAACTGGATGCCTTTACAGCTTTCGGCGGGTATTACCTTATTTAGCTTATTCTTATTAAGTCTAGGTGTTTACGGTTTGATTTTGCCGTTAGAGCGCAAAATCCGACAGGTGCGTTATGCATTAAACCGTATGAAATCGGGTGATTTGTCACTGCGTGTTCCTATTGAGGGAAGCGATGAAATGGCAAACTTGGCTTCAAGTTATAACAATATGTCTGACCATATTCAGCGTTTGATTGAGGCTCAACGTGAGTTAATGAGAGCAGTATCTCATGAACTCAGAACACCTGTGGCACGTATTCGCTTTGGTACAGAAATGTTAGCCGAAGAAGATGATTACAATCATCGTATGCATCAGGTCGACATGATTGATAAAGATATTGAAGCGCTCAATACCTTAATTGATGAAATCATGACTTATGCAAAACTAGAGCAGGGTACACCTTCATTAGACTTTGCAGAAATTGTACTTTTTGAAGTGTTGGATCAGGTTGCAGTTGAAACTGAGGCTTTAAAAACACAGAAAGAAATTGAACTTATTCCACCGCCTTTATATGTGAAAGTAGATGCAGAGCGTCGTTACCTTCACCGTGTCGTGCAAAATCTAGTAGGTAATGCAGTTCGTTATTGTGATAACAAAGTTCGGATTACGGGCGGTATTCATAATGATGGCATGGCTTTTGTCTGTGTCGAAGACGACGGACCGGGTATTCCTGAGCAAGACCGCAAGCGTGTATTTGAAGCCTTTGCCCGTTTAGATGATAGCCGTACACGTGCATCTGGCGGTTATGGTTTGGGTCTCTCTATCGTAAGTCGTATTGCTTACTGGTTTGGTGGGGAAATTAAGGTAGATGAAAGTCCAAGTTTGGGAGGCGCACGCTTTATTATGACTTGGCCAGCACATCGCTTTAAACAACCGCCATTAAAGACCAATAAAAAAGCACCTGCA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 1,657 total letters Query= IDP95892.106 Length=2052 Score E Sequences producing significant alignments: (Bits) Value wt 2971 0.0 V F K H S I F L R I Y A G L V I L V V L V A V F G Y L L V Q Query_1 202 GTGTTTAAACACAGTATATTCCTGCGAATATATGCGGGGCTGGTAATTCTTGTTGTTTTGGTAGCTGTTTTTGGGTATTTACTGGTACAA 291 2 1 .......................................................................................... 90 2 12 ............ 1 I I N Y Q R A Q E Y R E S L T D G I S Y V I S E G V A R Q P Query_1 292 ATTATCAACTACCAACGCGCACAAGAATACCGAGAATCTTTAACTGATGGTATTTCTTATGTCATTAGTGAAGGAGTCGCTCGACAACCT 381 2 91 .......................................................................................... 180 G K Q Q K I D W I S D A S D L L E L P I Y Y T D A S K V E L Query_1 382 GGGAAGCAGCAAAAAATAGATTGGATTTCTGACGCATCTGATTTGCTCGAACTTCCAATTTATTATACCGATGCAAGTAAGGTTGAGCTG 471 2 181 .......................................................................................... 270 S R T E K K R I E A Q K S V V R Y D A S N S I A Y V I I G L Query_1 472 TCTCGGACCGAGAAAAAGCGGATAGAAGCACAAAAATCTGTAGTTCGTTACGATGCAAGCAATAGCATTGCATATGTCATTATTGGCTTA 561 2 271 .......................................................................................... 360 2 443 ........... 433 R D D P Q H Y L S I K V D K I T E R Q M K A L P I F V L D Y Query_1 562 CGTGACGACCCACAACATTACTTATCTATAAAAGTCGACAAAATTACTGAACGCCAAATGAAGGCTCTTCCTATTTTTGTGCTCGACTAT 651 2 361 .......................................................................................... 450 2 307 ........... 297 L M F Y P G Q E Q E Y L A K I Q K H F S Y P I N I Q N I Q D Query_1 652 TTAATGTTTTATCCGGGGCAGGAACAAGAATATCTTGCCAAGATTCAAAAGCATTTCTCATATCCGATCAATATTCAAAATATTCAAGAT 741 2 451 .......................................................................................... 540 V N L D S E Q I G R L R Q D Q S V M L Y K D S A T V R G T T Query_1 742 GTAAATCTGGATTCTGAACAGATTGGACGTTTGCGCCAAGACCAAAGTGTCATGTTGTACAAAGATAGCGCAACAGTGCGCGGTACAACC 831 2 541 .......................................................................................... 630 I S I V S P I P N H P A Q V L V L G P V P M F N W M P L Q L Query_1 832 ATTTCGATTGTGTCTCCAATACCGAATCATCCTGCGCAAGTGTTAGTGCTGGGTCCGGTTCCAATGTTTAACTGGATGCCTTTACAGCTT 921 2 631 .......................................................................................... 720 S A G I T L F S L F L L S L G V Y G L I L P L E R K I R Q V Query_1 922 TCGGCGGGTATTACCTTATTTAGCTTATTCTTATTAAGTCTAGGTGTTTACGGTTTGATTTTGCCGTTAGAGCGCAAAATCCGACAGGTG 1011 2 721 .......................................................................................... 810 R Y A L N R M K S G D L S L R V P I E G S D E M A N L A S S Query_1 1012 CGTTATGCATTAAACCGTATGAAATCGGGTGATTTGTCACTGCGTGTTCCTATTGAGGGAAGCGATGAAATGGCAAACTTGGCTTCAAGT 1101 2 811 .......................................................................................... 900 Y N N M S D H I Q R L I E A Q R E L M R A V S H E L R T P V Query_1 1102 TATAACAATATGTCTGACCATATTCAGCGTTTGATTGAGGCTCAACGTGAGTTAATGAGAGCAGTATCTCATGAACTCAGAACACCTGTG 1191 2 901 .......................................................................................... 990 A R I R F G T E M L A E E D D Y N H R M H Q V D M I D K D I Query_1 1192 GCACGTATTCGCTTTGGTACAGAAATGTTAGCCGAAGAAGATGATTACAATCATCGTATGCATCAGGTCGACATGATTGATAAAGATATT 1281 2 991 .......................................................................................... 1080 E A L N T L I D E I M T Y A K L E Q G T P S L D F A E I V L Query_1 1282 GAAGCGCTCAATACCTTAATTGATGAAATCATGACTTATGCAAAACTAGAGCAGGGTACACCTTCATTAGACTTTGCAGAAATTGTACTT 1371 2 1081 .......................................................................................... 1170 F E V L D Q V A V E T E A L K T Q K E I E L I P P P L Y V K Query_1 1372 TTTGAAGTGTTGGATCAGGTTGCAGTTGAAACTGAGGCTTTAAAAACACAGAAAGAAATTGAACTTATTCCACCGCCTTTATATGTGAAA 1461 2 1171 .......................................................................................... 1260 V D A E R R Y L H R V V Q N L V G N A V R Y C D N K V R I T Query_1 1462 GTAGATGCAGAGCGTCGTTACCTTCACCGTGTCGTGCAAAATCTAGTAGGTAATGCAGTTCGTTATTGTGATAACAAAGTTCGGATTACG 1551 2 1261 .......................................................................................... 1350 G G I H N D G M A F V C V E D D G P G I P E Q D R K R V F E Query_1 1552 GGCGGTATTCATAATGATGGCATGGCTTTTGTCTGTGTCGAAGACGACGGACCGGGTATTCCTGAGCAAGACCGCAAGCGTGTATTTGAA 1641 2 1351 .......................................................................................... 1440 A F A R L D D S R T R A S G G Y G L G L S I V S R I A Y W F Query_1 1642 GCCTTTGCCCGTTTAGATGATAGCCGTACACGTGCATCTGGCGGTTATGGTTTGGGTCTCTCTATCGTAAGTCGTATTGCTTACTGGTTT 1731 2 1441 .......................................................................................... 1530 G G E I K V D E S P S L G G A R F I M T W P A H R F K Q P P Query_1 1732 GGTGGGGAAATTAAGGTAGATGAAAGTCCAAGTTTGGGAGGCGCACGCTTTATTATGACTTGGCCAGCACATCGCTTTAAACAACCGCCA 1821 2 1531 .......................................................................................... 1620 L K T N K K A P A Query_1 1822 TTAAAGACCAATAAAAAAGCACCTGCA 1848 2 1621 ........................... 1647 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3275924 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 1,657 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95892.107 (IDP95892.30-549.P(549)..pMCSG73)
QIINYQRAQEYRESLTDGISYVISEGVARQPGKQQKIDWISDASDLLELPIYYTDASKVELSRTEKKRIEAQKSVVRYDASNSIAYVIIGLRDDPQHYLSIKVDKITERQMKALPIFVLDYLMFYPGQEQEYLAKIQKHFSYPINIQNIQDVNLDSEQIGRLRQDQSVMLYKDSATVRGTTISIVSPIPNHPAQVLVLGPVPMFNWMPLQLSAGITLFSLFLLSLGVYGLILPLERKIRQVRYALNRMKSGDLSLRVPIEGSDEMANLASSYNNMSDHIQRLIEAQRELMRAVSHELRTPVARIRFGTEMLAEEDDYNHRMHQVDMIDKDIEALNTLIDEIMTYAKLEQGTPSLDFAEIVLFEVLDQVAVETEALKTQKEIELIPPPLYVKVDAERRYLHRVVQNLVGNAVRYCDNKVRITGGIHNDGMAFVCVEDDGPGIPEQDRKRVFEAFARLDDSRTRASGGYGLGLSIVSRIAYWFGGEIKVDESPSLGGARFIMTWPAHRFKQPPLKTNKKAPA
CAAATTATCAACTACCAACGCGCACAAGAATACCGAGAATCTTTAACTGATGGTATTTCTTATGTCATTAGTGAAGGAGTCGCTCGACAACCTGGGAAGCAGCAAAAAATAGATTGGATTTCTGACGCATCTGATTTGCTCGAACTTCCAATTTATTATACCGATGCAAGTAAGGTTGAGCTGTCTCGGACCGAGAAAAAGCGGATAGAAGCACAAAAATCTGTAGTTCGTTACGATGCAAGCAATAGCATTGCATATGTCATTATTGGCTTACGTGACGACCCACAACATTACTTATCTATAAAAGTCGACAAAATTACTGAACGCCAAATGAAGGCTCTTCCTATTTTTGTGCTCGACTATTTAATGTTTTATCCGGGGCAGGAACAAGAATATCTTGCCAAGATTCAAAAGCATTTCTCATATCCGATCAATATTCAAAATATTCAAGATGTAAATCTGGATTCTGAACAGATTGGACGTTTGCGCCAAGACCAAAGTGTCATGTTGTACAAAGATAGCGCAACAGTGCGCGGTACAACCATTTCGATTGTGTCTCCAATACCGAATCATCCTGCGCAAGTGTTAGTGCTGGGTCCGGTTCCAATGTTTAACTGGATGCCTTTACAGCTTTCGGCGGGTATTACCTTATTTAGCTTATTCTTATTAAGTCTAGGTGTTTACGGTTTGATTTTGCCGTTAGAGCGCAAAATCCGACAGGTGCGTTATGCATTAAACCGTATGAAATCGGGTGATTTGTCACTGCGTGTTCCTATTGAGGGAAGCGATGAAATGGCAAACTTGGCTTCAAGTTATAACAATATGTCTGACCATATTCAGCGTTTGATTGAGGCTCAACGTGAGTTAATGAGAGCAGTATCTCATGAACTCAGAACACCTGTGGCACGTATTCGCTTTGGTACAGAAATGTTAGCCGAAGAAGATGATTACAATCATCGTATGCATCAGGTCGACATGATTGATAAAGATATTGAAGCGCTCAATACCTTAATTGATGAAATCATGACTTATGCAAAACTAGAGCAGGGTACACCTTCATTAGACTTTGCAGAAATTGTACTTTTTGAAGTGTTGGATCAGGTTGCAGTTGAAACTGAGGCTTTAAAAACACAGAAAGAAATTGAACTTATTCCACCGCCTTTATATGTGAAAGTAGATGCAGAGCGTCGTTACCTTCACCGTGTCGTGCAAAATCTAGTAGGTAATGCAGTTCGTTATTGTGATAACAAAGTTCGGATTACGGGCGGTATTCATAATGATGGCATGGCTTTTGTCTGTGTCGAAGACGACGGACCGGGTATTCCTGAGCAAGACCGCAAGCGTGTATTTGAAGCCTTTGCCCGTTTAGATGATAGCCGTACACGTGCATCTGGCGGTTATGGTTTGGGTCTCTCTATCGTAAGTCGTATTGCTTACTGGTTTGGTGGGGAAATTAAGGTAGATGAAAGTCCAAGTTTGGGAGGCGCACGCTTTATTATGACTTGGCCAGCACATCGCTTTAAACAACCGCCATTAAAGACCAATAAAAAAGCACCTGCA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 1,721 total letters Query= IDP95892.107 Length=1965 Score E Sequences producing significant alignments: (Bits) Value wt 2814 0.0 01C_C7_IDP95892.107_v73F.seq 24.7 0.12 L Y F Q S N A Q I I N Y Q R A Q E Y R E S L T D G I S Y V Query_1 179 ACCTGTACTTCCAATCCAATGCCCAAATTATCAACTACCAACGCGCACAAGAATACCGAGAATCTTTAACTGATGGTATTTCTTATGTCA 268 2 1 ................................................................... 67 0 64 ............. 76 I S E G V A R Q P G K Q Q K I D W I S D A S D L L E L P I Y Query_1 269 TTAGTGAAGGAGTCGCTCGACAACCTGGGAAGCAGCAAAAAATAGATTGGATTTCTGACGCATCTGATTTGCTCGAACTTCCAATTTATT 358 2 68 .......................................................................................... 157 Y T D A S K V E L S R T E K K R I E A Q K S V V R Y D A S N Query_1 359 ATACCGATGCAAGTAAGGTTGAGCTGTCTCGGACCGAGAAAAAGCGGATAGAAGCACAAAAATCTGTAGTTCGTTACGATGCAAGCAATA 448 2 158 .......................................................................................... 247 2 356 ........... 346 S I A Y V I I G L R D D P Q H Y L S I K V D K I T E R Q M K Query_1 449 GCATTGCATATGTCATTATTGGCTTACGTGACGACCCACAACATTACTTATCTATAAAAGTCGACAAAATTACTGAACGCCAAATGAAGG 538 2 248 .......................................................................................... 337 A L P I F V L D Y L M F Y P G Q E Q E Y L A K I Q K H F S Y Query_1 539 CTCTTCCTATTTTTGTGCTCGACTATTTAATGTTTTATCCGGGGCAGGAACAAGAATATCTTGCCAAGATTCAAAAGCATTTCTCATATC 628 2 338 .......................................................................................... 427 2 220 ........... 210 P I N I Q N I Q D V N L D S E Q I G R L R Q D Q S V M L Y K Query_1 629 CGATCAATATTCAAAATATTCAAGATGTAAATCTGGATTCTGAACAGATTGGACGTTTGCGCCAAGACCAAAGTGTCATGTTGTACAAAG 718 2 428 .......................................................................................... 517 D S A T V R G T T I S I V S P I P N H P A Q V L V L G P V P Query_1 719 ATAGCGCAACAGTGCGCGGTACAACCATTTCGATTGTGTCTCCAATACCGAATCATCCTGCGCAAGTGTTAGTGCTGGGTCCGGTTCCAA 808 2 518 .......................................................................................... 607 M F N W M P L Q L S A G I T L F S L F L L S L G V Y G L I L Query_1 809 TGTTTAACTGGATGCCTTTACAGCTTTCGGCGGGTATTACCTTATTTAGCTTATTCTTATTAAGTCTAGGTGTTTACGGTTTGATTTTGC 898 2 608 .......................................................................................... 697 P L E R K I R Q V R Y A L N R M K S G D L S L R V P I E G S Query_1 899 CGTTAGAGCGCAAAATCCGACAGGTGCGTTATGCATTAAACCGTATGAAATCGGGTGATTTGTCACTGCGTGTTCCTATTGAGGGAAGCG 988 2 698 .......................................................................................... 787 D E M A N L A S S Y N N M S D H I Q R L I E A Q R E L M R A Query_1 989 ATGAAATGGCAAACTTGGCTTCAAGTTATAACAATATGTCTGACCATATTCAGCGTTTGATTGAGGCTCAACGTGAGTTAATGAGAGCAG 1078 2 788 .......................................................................................... 877 V S H E L R T P V A R I R F G T E M L A E E D D Y N H R M H Query_1 1079 TATCTCATGAACTCAGAACACCTGTGGCACGTATTCGCTTTGGTACAGAAATGTTAGCCGAAGAAGATGATTACAATCATCGTATGCATC 1168 2 878 .......................................................................................... 967 Q V D M I D K D I E A L N T L I D E I M T Y A K L E Q G T P Query_1 1169 AGGTCGACATGATTGATAAAGATATTGAAGCGCTCAATACCTTAATTGATGAAATCATGACTTATGCAAAACTAGAGCAGGGTACACCTT 1258 2 968 .......................................................................................... 1057 S L D F A E I V L F E V L D Q V A V E T E A L K T Q K E I E Query_1 1259 CATTAGACTTTGCAGAAATTGTACTTTTTGAAGTGTTGGATCAGGTTGCAGTTGAAACTGAGGCTTTAAAAACACAGAAAGAAATTGAAC 1348 2 1058 .......................................................................................... 1147 L I P P P L Y V K V D A E R R Y L H R V V Q N L V G N A V R Query_1 1349 TTATTCCACCGCCTTTATATGTGAAAGTAGATGCAGAGCGTCGTTACCTTCACCGTGTCGTGCAAAATCTAGTAGGTAATGCAGTTCGTT 1438 2 1148 .......................................................................................... 1237 Y C D N K V R I T G G I H N D G M A F V C V E D D G P G I P Query_1 1439 ATTGTGATAACAAAGTTCGGATTACGGGCGGTATTCATAATGATGGCATGGCTTTTGTCTGTGTCGAAGACGACGGACCGGGTATTCCTG 1528 2 1238 .......................................................................................... 1327 E Q D R K R V F E A F A R L D D S R T R A S G G Y G L G L S Query_1 1529 AGCAAGACCGCAAGCGTGTATTTGAAGCCTTTGCCCGTTTAGATGATAGCCGTACACGTGCATCTGGCGGTTATGGTTTGGGTCTCTCTA 1618 2 1328 .......................................................................................... 1417 I V S R I A Y W F G G E I K V D E S P S L G G A R F I M T W Query_1 1619 TCGTAAGTCGTATTGCTTACTGGTTTGGTGGGGAAATTAAGGTAGATGAAAGTCCAAGTTTGGGAGGCGCACGCTTTATTATGACTTGGC 1708 2 1418 .......................................................................................... 1507 P A H R F K Q P P L K T N K K A P A Query_1 1709 CAGCACATCGCTTTAAACAACCGCCATTAAAGACCAATAAAAAAGCACCTGCA 1761 2 1508 ..................................................... 1560 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3260677 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 1,721 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95892.108 (IDP95892.30-240.P(549)..pMCSG73)
QIINYQRAQEYRESLTDGISYVISEGVARQPGKQQKIDWISDASDLLELPIYYTDASKVELSRTEKKRIEAQKSVVRYDASNSIAYVIIGLRDDPQHYLSIKVDKITERQMKALPIFVLDYLMFYPGQEQEYLAKIQKHFSYPINIQNIQDVNLDSEQIGRLRQDQSVMLYKDSATVRGTTISIVSPIPNHPAQVLVLGPVPMFNWMPLQL
CAAATTATCAACTACCAACGCGCACAAGAATACCGAGAATCTTTAACTGATGGTATTTCTTATGTCATTAGTGAAGGAGTCGCTCGACAACCTGGGAAGCAGCAAAAAATAGATTGGATTTCTGACGCATCTGATTTGCTCGAACTTCCAATTTATTATACCGATGCAAGTAAGGTTGAGCTGTCTCGGACCGAGAAAAAGCGGATAGAAGCACAAAAATCTGTAGTTCGTTACGATGCAAGCAATAGCATTGCATATGTCATTATTGGCTTACGTGACGACCCACAACATTACTTATCTATAAAAGTCGACAAAATTACTGAACGCCAAATGAAGGCTCTTCCTATTTTTGTGCTCGACTATTTAATGTTTTATCCGGGGCAGGAACAAGAATATCTTGCCAAGATTCAAAAGCATTTCTCATATCCGATCAATATTCAAAATATTCAAGATGTAAATCTGGATTCTGAACAGATTGGACGTTTGCGCCAAGACCAAAGTGTCATGTTGTACAAAGATAGCGCAACAGTGCGCGGTACAACCATTTCGATTGTGTCTCCAATACCGAATCATCCTGCGCAAGTGTTAGTGCTGGGTCCGGTTCCAATGTTTAACTGGATGCCTTTACAGCTT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,392 total letters Query= IDP95892.108 Length=1038 Score E Sequences producing significant alignments: (Bits) Value 09H_D7_IDP95892.108_T7term.seq 1198 0.0 wt 1142 0.0 01D_D7_IDP95892.108_v73F.seq 901 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTT-CGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAA 197 1 755 ...........A.....T...G....------...................... 708 1 773 .....C........... 757 0 44 ...........A.....-...G....------...............N....N. 90 0 28 ...C........... 42 0 29 .............. 42 N A Q I I N Y Q R A Q E Y R E S L T D G I S Y V I S E G V A Query_1 198 TGCCCAAATTATCAACTACCAACGCGCACAAGAATACCGAGAATCTTTAACTG-ATGGTATTTCTTATGTCATTAGTGAAGGAGTCGCTC 286 1 707 .....................................................N.................................... 618 2 1 .................................................-.................................... 85 0 91 .....................................................-.................................... 179 R Q P G K Q Q K I D W I S D A S D L L E L P I Y Y T D A S K Query_1 287 GACAACCTGGG-AAGCAGCAAAAAATAGATTGGATTTCTGACGCATCTGATTTGCTCGAACTTCCAATTTATTATACCGATGCAAGTAAG 375 1 617 ...........G...............................................................N.............. 528 2 86 ...........-.............................................................................. 174 0 180 ...........-.............................................................................. 268 V E L S R T E K K R I E A Q K S V V R Y D A S N S I A Y V I Query_1 376 GTTGAGCTGTCTCGGACCGAGAAAAAGCGGATAGAAGCACAAAAATCTGTAGTTCGTTACGATGCAAGCAATAGCATTGCATATGTCATT 465 1 527 .......................................................................................... 438 1 346 ........... 356 2 175 .......................................................................................... 264 2 356 ........... 346 0 269 .......................................................................................... 358 0 450 ........... 440 I G L R D D P Q H Y L S I K V D K I T E R Q M K A L P I F V Query_1 466 ATTGGCTTACGTGACGACCCACAACATTACTTATCTATAAAAGTCGACAAAATTACTGAACGCCAAATGAAGGCTCTTCCTATTTTTGTG 555 1 437 .......................................................................................... 348 1 482 ......... 490 2 265 .......................................................................................... 354 2 220 ......... 212 0 359 .......................................................................................... 448 0 314 ......... 306 L D Y L M F Y P G Q E Q E Y L A K I Q K H F S Y P I N I Q N Query_1 556 CTCGACTATTTAATGTTTTATCCGGGGCAGGAACAAGAATATCTTGCCAAGATTCAAAAGCATTTCTCATATCCGATCAATATTCAAAAT 645 1 347 ...........................G.............................................................. 258 1 491 .. 492 2 355 .......................................................................................... 444 2 211 .. 210 0 449 ...........................G.............................................................. 538 0 305 .. 304 I Q D V N L D S E Q I G R L R Q D Q S V M L Y K D S A Query_1 646 A-TTCAAGATGTAAA--TCTGGATT-CTGAA-CAGATTGGA-CGTTTGCGCCAAG-ACCAAAGTGTCATGTTGTACAAAGATAGCGCAAC 728 1 257 .-.............--........-.....-.........-.............-.................................. 175 2 445 .-.............--........-.....-.........-.............-.................................. 527 0 539 .T.............ATC.......C.....C..AT.....C.T...........A....... 601 T V R G T T I S I V S P I P N H P A Q V L V L G P V P M F N Query_1 729 AGTGCGCGGTACAACCATTTCGATTGTGTCTCCAATACCGAATCATCCTGCGCAAGTGTTAGTGCTGGGTCCGGTTCCAATGTTTAACTG 818 1 174 ........................................................................AC...........T.N.A 85 2 528 .......................................................................................... 617 W M P L Q L * H W K W I T D P N S S S V D K L A A A L E H H Query_1 819 GATGCCTTTACAGCTTTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccacca 908 1 84 ....................................--..... 44 1 91 ............... 105 2 618 ................ 633 H H H H * D P A A N K A R Query_1 909 ccaccaccacTGAGATCCGGCTGCTAAC-AAAGCCCGAAA 947 1 48 ..............A........... 23 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2400981 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 2,392 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95893.103 (IDP95893.1-290.F..pMCSG73)
MKKFLGAYQNNHMHWVGDGFPVYNLFSYDRLGQTLSPFLLLDYAAPYNFSPTTEQQGVGSHPHRGFETVTIAYQGEVTHKDSSGGGGTIKTGDVQWMTAGAGVLHEEFHSPEFAEHGGLFEMVQLWVNLPSHSKMTPGKYQAIEAKDIPDIALDEHGSHLRVIAGEYADAKGAATTFSPLNVWDGKLVKGQKHTLYVPEGHTTLVVVLEGAVVVNDTNRLEGKTVAILSREGVEFSLNAEEDTKFLVLTGQPLNEPIEGYGPFVMNTKAEIMEAINDFNRGKFGSIMQEG
ATGAAGAAATTTCTTGGTGCATATCAAAATAACCACATGCATTGGGTTGGTGATGGTTTCCCTGTATATAACCTGTTCTCTTATGACCGTTTAGGCCAAACCCTAAGCCCATTTTTGTTACTGGATTATGCAGCGCCATATAACTTCTCACCAACCACCGAACAACAAGGTGTGGGTTCACATCCTCACCGTGGCTTCGAAACAGTTACTATTGCCTATCAAGGTGAAGTGACTCATAAAGACTCAAGTGGTGGTGGCGGAACCATTAAAACCGGTGACGTGCAATGGATGACTGCGGGTGCAGGGGTGTTACATGAAGAGTTCCATTCTCCTGAATTTGCAGAGCATGGCGGTCTTTTTGAAATGGTGCAATTGTGGGTAAATTTACCTTCGCATTCAAAAATGACCCCTGGAAAATATCAAGCCATTGAAGCTAAAGATATTCCGGACATTGCACTAGACGAGCACGGTAGTCATTTGCGTGTGATCGCAGGTGAATATGCAGATGCAAAAGGTGCAGCAACTACATTTAGTCCATTAAATGTATGGGACGGTAAATTGGTAAAAGGCCAAAAACATACCCTGTATGTACCTGAAGGGCATACTACCCTTGTTGTTGTGCTAGAGGGTGCGGTTGTGGTCAATGATACAAACCGTCTTGAAGGCAAAACCGTTGCGATTTTATCTCGTGAGGGCGTTGAGTTTAGCTTAAATGCTGAAGAAGACACTAAATTCTTAGTGTTGACTGGCCAACCACTAAATGAGCCAATCGAAGGTTATGGTCCATTTGTCATGAATACCAAAGCCGAGATTATGGAAGCGATTAATGATTTTAATCGTGGAAAATTCGGTTCAATCATGCAAGAAGGA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 1,637 total letters Query= IDP95893.103 Length=1275 Score E Sequences producing significant alignments: (Bits) Value wt 1570 0.0 01E_E7_IDP95893.103_v73F.seq 738 0.0 05E_E7_IDP95893.103_T7term.seq 329 1e-93 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 0 68 ..........T....A..CG---. 88 0 28 ...C........... 42 0 29 .............. 42 A M K K F L G A Y Q N N H M H W V G D G F P V Y N L F S Y D Query_1 199 GCCATGAAGAAATTTCTTGGTGCATATCAAAATAACCACATGCATTGGGTTGGTGATGGTTTCCCTGTATATAACCTGTTCTCTTATGAC 288 2 1 ....................................................................................... 87 2 15 ............ 4 0 89 .....................................N.T..N............................................... 178 0 106 ............ 95 R L G Q T L S P F L L L D Y A A P Y N F S P T T E Q Q G V G Query_1 289 CGTTTAGGCCAAACCCTAAGCCCATTTTTGTTACTGGATTATGCAGCGCCATATAACTTCTCACCAACCACCGAACAACAAGGTGTGGGT 378 2 88 .......................................................................................... 177 0 179 .............................................N............................................ 268 S H P H R G F E T V T I A Y Q G E V T H K D S S G G G G T I Query_1 379 TCACATCCTCACCGTGGCTTCGAAACAGTTACTATTGCCTATCAAGGTGAAGTGACTCATAAAGACTCAAGTGGTGGTGGCGGAACCATT 468 2 178 .......................................................................................... 267 0 269 .................N.................................N..............................N....... 358 K T G D V Q W M T A G A G V L H E E F H S P E F A E H G G L Query_1 469 AAAACCGGTGACGTGCAATGGATGACTGCGGGTGCAGGGGTGTTACATGAAGAGTTCCATTCTCCTGAATTTGCAGAGCATGGCGGTCTT 558 2 268 .......................................................................................... 357 0 359 ...................................NN......................................N......T....... 448 F E M V Q L W V N L P S H S K M T P G K Y Q A I E A K D I P Query_1 559 TTTGAAATGGTGCAATTGTGGGTAAATTTACCTTCGCATTCAAAAATGACCCCTGGAAAATATCAAGCCATTGAAGCTAAAGATATTCCG 648 2 358 .......................................................................................... 447 2 389 ............ 378 0 449 ...................................T............................N....T.... 522 0 480 ............ 469 D I A L D E H G S H L R V I A G E Y A D A K G A A T T F S P Query_1 649 GACATTGCACTAGACGAGCACGGTAGTCATTTGCGTGTGATCGCAGGTGAATATGCAGATGCAAAAGGTGCAGCAACTACATTTAGTCCA 738 2 448 .......................................................................................... 537 L N V W D G K L V K G Q K H T L Y V P E G H T T L V V V L E Query_1 739 TTAAATGTATGGGACGGTAAATTGGTAAAAGGCCAAAAACATACCCTGTATGTACCTGAAGGGCATACTACCCTTGTTGTTGTGCTAGAG 828 2 538 .......................................................................................... 627 G A V V V N D T N R L E G K T V A I L S R E G V E F S L N A Query_1 829 GGTGCGGTTGTGGTCAATGATACAAACCGTCTTGAAGGCAAAACCGTTGCGATTTTATCTCGTGAGGGCGTTGAGTTTAGCTTAAATGCT 918 2 628 .......................................................................................... 717 1 245 ............................. 217 E E D T K F L V L T G Q P L N E P I E G Y G P F V M N T K A Query_1 919 GAAGAAGACACTAAATTCTTAGTGTTGACTGGCCAACCACTAAATGAGCCAATCGAAGGTTATGGTCCATTTGTCATGAATACCAAAGCC 1008 2 718 .......................................................................................... 807 1 216 .................A.......N............NN...............................................A.. 127 E I M E A I N D F N R G K F G S I M Q E G * H W K W I T D P Query_1 1009 GAGATTATGGAAGCGATTAATGATTTTAATCGTGGAAAATTCGGTTCAATCATGCAAGAAGGATAACATTGGAAGTGGATAACGGATCCG 1098 2 808 ............................................................... 870 1 126 ......................................CA.T...........................N.............--..... 39 N S S S V D K L A A A L E H H H H H H * D P A A N K A R K Query_1 1099 AATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAA-AGCCCGAAAGGA 1187 1 43 ......N......A..N....A....A.. 15 E Query_1 1188 A 1188 1 14 . 14 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2005920 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 1,637 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95895.104 (IDP95895.1-394.F..pMCSG73)
MLQHNIYKAFFVSVLMCCSQLVFSAPVVKEKLSPLHPDSPESEESYAEVPIESIQQFVQIYGIVRDNYVDEKSDDALFLQAIKGLVSGLDRYSRYLSAEEYRQLIQYTEGDLASVDFVLSPESHVHKWMIRDLKTGSDSYKLGLRNGQTILKIDNQELKNLTHDQVLGLLYGSIGSTLQVQTEESNSPISLVRNKKIETDIEPVMLHNQVLVLKIRVFQQDTANEIKRLIEENSSSRLKAVLIDLRNNPGGLLSAAVESADLFLNHGIIVSTKSRSEGNQQFQALPGNDFQNIKVGILINHRSASAAEVFTAAMKEHQRAWVMGEKSYGKGVVQKLFPLPSGAALQMTVSHYYTPNGNMIEGQGIQPNQTYPLPPEMKEEVYLDRVADLLLKRK
ATGCTGCAACACAATATATATAAAGCTTTTTTTGTTAGCGTATTGATGTGTTGCAGCCAGCTCGTTTTTTCAGCTCCTGTTGTAAAAGAAAAATTATCGCCTCTGCATCCTGATTCCCCGGAATCAGAAGAAAGTTATGCAGAAGTGCCTATTGAGTCTATTCAACAGTTTGTACAAATATATGGGATTGTCCGTGATAATTATGTCGATGAAAAATCGGACGATGCTTTATTTTTACAGGCAATTAAAGGTTTAGTCAGTGGTCTAGACCGTTACTCCCGCTATTTGTCAGCTGAAGAATACCGTCAACTCATTCAATATACTGAAGGGGATCTTGCTTCAGTTGATTTTGTACTAAGCCCCGAATCACATGTTCATAAATGGATGATTCGTGACCTTAAGACGGGTTCTGACTCATATAAACTAGGCTTAAGAAATGGCCAAACTATTTTAAAGATTGATAATCAGGAACTTAAAAATCTGACACATGATCAGGTGCTAGGTTTGCTTTATGGATCGATAGGCTCGACGCTTCAAGTACAAACAGAAGAGTCAAATAGTCCAATTAGTTTAGTCCGTAATAAAAAGATTGAAACCGATATTGAGCCTGTGATGTTGCACAATCAGGTGTTGGTATTAAAAATTAGAGTATTTCAACAAGATACCGCCAATGAGATTAAGCGTTTAATTGAAGAAAATAGTTCTTCGCGTTTAAAAGCGGTGTTAATTGATTTAAGAAATAACCCAGGCGGCTTGTTATCTGCTGCTGTTGAGTCAGCCGATTTATTTTTAAATCATGGCATTATTGTCTCTACAAAAAGTCGTTCAGAAGGCAATCAGCAATTTCAGGCTTTGCCAGGTAACGATTTTCAAAATATAAAAGTTGGTATTTTAATTAATCACCGATCTGCCTCGGCAGCAGAAGTTTTTACTGCTGCAATGAAAGAGCATCAACGTGCTTGGGTCATGGGTGAAAAAAGTTATGGAAAGGGTGTTGTGCAAAAGCTTTTCCCTCTACCAAGTGGGGCAGCGCTACAAATGACAGTCTCACATTACTACACACCAAATGGTAATATGATTGAGGGGCAAGGTATTCAGCCTAACCAGACTTATCCCTTACCCCCAGAAATGAAAGAAGAAGTTTATCTAGATCGTGTTGCCGATCTCCTTCTAAAAAGAAAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,386 total letters Query= IDP95895.104 Length=1587 Score E Sequences producing significant alignments: (Bits) Value wt 2132 0.0 01F_F7_IDP95895.104_v73F.seq 1187 0.0 05F_F7_IDP95895.104_T7term.seq 706 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 0 40 ...........A........G....------....................... 87 0 24 ...C........... 38 0 25 .............. 38 A M L Q H N I Y K A F F V S V L M C C S Q L V F S A P V V K Query_1 199 GCCATGCTGCAACACAATATATATAAAGCtttttttGTTAGCGTATTGATGTGTTGCAGCCAGCTCGTTTTTTCAGCTCCTGTTGTAAAA 288 2 1 ....................................................................................... 87 2 13 ........... 3 2 57 ........... 47 0 88 .......................................................................................... 177 0 103 ........... 93 0 147 ........... 137 E K L S P L H P D S P E S E E S Y A E V P I E S I Q Q F V Q Query_1 289 GAAAAATTATCGCCTCTGCATCCTGATTCCCCGGAATCAGAAGAAAGTTATGCAGAAGTGCCTATTGAGTCTATTCAACAGTTTGTACAA 378 2 88 .......................................................................................... 177 0 178 .......................................................................................... 267 I Y G I V R D N Y V D E K S D D A L F L Q A I K G L V S G L Query_1 379 ATATATGGGATTGTCCGTGATAATTATGTCGATGAAAAATCGGACGATGCTTTATTTTTACAGGCAATTAAAGGTTTAGTCAGTGGTCTA 468 2 178 .......................................................................................... 267 0 268 .......................................................................................... 357 D R Y S R Y L S A E E Y R Q L I Q Y T E G D L A S V D F V L Query_1 469 GACCGTTACTCCCGCTATTTGTCAGCTGAAGAATACCGTCAACTCATTCAATATACTGAAGGGGATCTTGCTTCAGTTGATTTTGTACTA 558 2 268 .......................................................................................... 357 0 358 .......................................................................................... 447 S P E S H V H K W M I R D L K T G S D S Y K L G L R N G Q T Query_1 559 AGCCCCGAATCACATGTTCATAAATGGATGATTCGTGACCTTAAGACGGGTTCTGACTCATATAAACTAGGCTTAAGAAATGGCCAAACT 648 2 358 .......................................................................................... 447 0 448 .......................................................................................... 537 I L K I D N Q E L K N L T H D Q V L G L L Y G S I G S T L Q Query_1 649 ATTTTAAAGATTGATAATCAGGAACTTAAAAATCTGACACATGATCAGGTGCTAGGTTTGCTTTATGGATCGATAGGCTCGACGCTTCAA 738 2 448 .......................................................................................... 537 0 538 .......................................................................................... 627 V Q T E E S N S P I S L V R N K K I E T D I E P V M L H N Query_1 739 GTACAAACAGAAGAGTCAAATAGTCCAATTAGTTTAGTCCGTAATAAAAAGATTG-AAACCGATATTGAGCCTGTGATGTTGCACAATCA 827 2 538 .......................................................-.................................. 626 0 628 ........N...........................N..................A.................................. 717 Q V L V L K I R V F Q Q D T A N E I K R L I E E N S S S R L Query_1 828 GGTGTTGGTATTAAAAATTAGAGTATTTCAACAAGATACCGCCAATGAGATTAAGCGTTTAATTGAAGAAAATAGTTCTTCGCGTTTAAA 917 2 627 .......................................................................................... 716 0 718 .. 719 K A V L I D L R N N P G G L L S A A V E S A D L F L N H G I Query_1 918 AGCGGTGTTAATTGATTTAAGAAATAACCCAGGCGGCTTGTTATCTGCTGCTGTTGAGTCAGCCGATTTATTTTTAAATCATGG-CATTA 1006 2 717 ....................................................................................-..... 805 1 478 ..A...............N.......AG.AN.A..... 441 I V S T K S R S E G N Q Q F Q A L P G N D F Q N I K V G I L Query_1 1007 TTGTCTCTACAAAAAGTCGTTCAGAAGGCAATCAGCAATTTCAGGCTTTGCCAGGTAACGATTTTCAAAATATAAAAGTTGGTATTTTAA 1096 2 806 .......................................................................................... 895 1 440 ....N.....................A............................................................... 351 I N H R S A S A A E V F T A A M K E H Q R A W V M G E K S Y Query_1 1097 TTAATCACCGATCTGCCTCGGCAGCAGAAGTTTTTACTGCTGCAATGAAAGAGCATCAACGTGCTTGGGTCATGGGTGAAAAAAGTTATG 1186 2 896 .......................................................................................... 985 1 350 ....................................N..................................................... 261 G K G V V Q K L F P L P S G A A L Q M T V S H Y Y T P N G N Query_1 1187 GAAAGGGTGTTGTGCAAAAGCTTTTCCCTCTACCAAGTGGGGCAGCGCTACAAATGACAGTCTCACATTACTACACACCAAATGGTAATA 1276 2 986 .......................................................................................... 1075 1 260 .......................................................................................... 171 M I E G Q G I Q P N Q T Y P L P P E M K E E V Y L D R V A D Query_1 1277 TGATTGAGGGGCAAGGTATTCAGCCTAACCAGACTTATCCCTTACCCCCAGAAATGAAAGAAGAAGTTTATCTAGATCGTGTTGCCGA-T 1365 2 1076 ........................................................................................-. 1164 1 170 ..................................................................................--.TT.T. 83 L L L K R K * H W K W I T D P N S S S V D K L A A A L E H H Query_1 1366 CTCCTTCTAAAAAGAAAGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccac 1455 2 1165 .................. 1182 1 82 .......................-..............--..... 41 H H H H * D P A A N K A R K E Query_1 1456 caccaccaccacTGAGATCCGGCTGCTAAC-AAAGCCCG--AAAGGAAG 1501 1 45 ..............A......N.AA........ 13 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3672998 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 2,386 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95895.105 (IDP95895.25-394.P(394)..pMCSG73)
APVVKEKLSPLHPDSPESEESYAEVPIESIQQFVQIYGIVRDNYVDEKSDDALFLQAIKGLVSGLDRYSRYLSAEEYRQLIQYTEGDLASVDFVLSPESHVHKWMIRDLKTGSDSYKLGLRNGQTILKIDNQELKNLTHDQVLGLLYGSIGSTLQVQTEESNSPISLVRNKKIETDIEPVMLHNQVLVLKIRVFQQDTANEIKRLIEENSSSRLKAVLIDLRNNPGGLLSAAVESADLFLNHGIIVSTKSRSEGNQQFQALPGNDFQNIKVGILINHRSASAAEVFTAAMKEHQRAWVMGEKSYGKGVVQKLFPLPSGAALQMTVSHYYTPNGNMIEGQGIQPNQTYPLPPEMKEEVYLDRVADLLLKRK
GCTCCTGTTGTAAAAGAAAAATTATCGCCTCTGCATCCTGATTCCCCGGAATCAGAAGAAAGTTATGCAGAAGTGCCTATTGAGTCTATTCAACAGTTTGTACAAATATATGGGATTGTCCGTGATAATTATGTCGATGAAAAATCGGACGATGCTTTATTTTTACAGGCAATTAAAGGTTTAGTCAGTGGTCTAGACCGTTACTCCCGCTATTTGTCAGCTGAAGAATACCGTCAACTCATTCAATATACTGAAGGGGATCTTGCTTCAGTTGATTTTGTACTAAGCCCCGAATCACATGTTCATAAATGGATGATTCGTGACCTTAAGACGGGTTCTGACTCATATAAACTAGGCTTAAGAAATGGCCAAACTATTTTAAAGATTGATAATCAGGAACTTAAAAATCTGACACATGATCAGGTGCTAGGTTTGCTTTATGGATCGATAGGCTCGACGCTTCAAGTACAAACAGAAGAGTCAAATAGTCCAATTAGTTTAGTCCGTAATAAAAAGATTGAAACCGATATTGAGCCTGTGATGTTGCACAATCAGGTGTTGGTATTAAAAATTAGAGTATTTCAACAAGATACCGCCAATGAGATTAAGCGTTTAATTGAAGAAAATAGTTCTTCGCGTTTAAAAGCGGTGTTAATTGATTTAAGAAATAACCCAGGCGGCTTGTTATCTGCTGCTGTTGAGTCAGCCGATTTATTTTTAAATCATGGCATTATTGTCTCTACAAAAAGTCGTTCAGAAGGCAATCAGCAATTTCAGGCTTTGCCAGGTAACGATTTTCAAAATATAAAAGTTGGTATTTTAATTAATCACCGATCTGCCTCGGCAGCAGAAGTTTTTACTGCTGCAATGAAAGAGCATCAACGTGCTTGGGTCATGGGTGAAAAAAGTTATGGAAAGGGTGTTGTGCAAAAGCTTTTCCCTCTACCAAGTGGGGCAGCGCTACAAATGACAGTCTCACATTACTACACACCAAATGGTAATATGATTGAGGGGCAAGGTATTCAGCCTAACCAGACTTATCCCTTACCCCCAGAAATGAAAGAAGAAGTTTATCTAGATCGTGTTGCCGATCTCCTTCTAAAAAGAAAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,075 total letters Query= IDP95895.105 Length=1515 Score E Sequences producing significant alignments: (Bits) Value wt 2003 0.0 05G_G7_IDP95895.105_T7term.seq 1003 0.0 01G_G7_IDP95895.105_v73F.seq 448 3e-129 N L Y F Q S N A A P V V K E K L S P L H P D S P E S E E Query_1 176 AGAACCT-GTACTTCCA-ATCC-AAT-GCCGCTCCTGTTGTAAAAGAAAAATTATCGCCTCTGCATCCTGATTCCCCGGAATCAGAAGAA 261 2 1 ............................................................ 60 0 17 .......T........CC....C...N..............................T.........................T-..... 105 S Y A E V P I E S I Q Q F V Q I Y G I V R D N Y V D E K S D Query_1 262 AGTTATGCAGAAGTGCCTATTGAGTCTATTCAACAGTTTGTACAAATATATGGGATTGTCCGTGATAATTATGTCGATGAAAAATCGGAC 351 2 61 .......................................................................................... 150 0 106 ........N...N.........................................T..........................T...TN... 195 D A L F L Q A I K G L V S G L D R Y S R Y L S A E E Y R Q L Query_1 352 GATGCTTTATTTTTACAGGCAATTAAAGGTTTAGTCAGTGGTCTAGACCGTTACTCCCGCTATTTGTCAGCTGAAGAATACCGTCAACTC 441 2 151 .......................................................................................... 240 0 196 .............................................N.T.......................................... 285 I Q Y T E G D L A S V D F V L S P E S H V H K W M I R D L K Query_1 442 ATTCAATATACTGAAGGGGATCTTGCTTCAGTTGATTTTGTACTAAGCCCCGAATCACATGTTCATAAATGGATGATTCGTGACCTTAAG 531 2 241 .......................................................................................... 330 0 286 ....N............NA...........T.... 320 T G S D S Y K L G L R N G Q T I L K I D N Q E L K N L T H D Query_1 532 ACGGGTTCTGACTCATATAAACTAGGCTTAAGAAATGGCCAAACTATTTTAAAGATTGATAATCAGGAACTTAAAAATCTGACACATGAT 621 2 331 .......................................................................................... 420 Q V L G L L Y G S I G S T L Q V Q T E E S N S P I S L V R N Query_1 622 CAGGTGCTAGGTTTGCTTTATGGATCGATAGGCTCGACGCTTCAAGTACAAACAGAAGAGTCAAATAGTCCAATTAGTTTAGTCCGTAAT 711 2 421 .......................................................................................... 510 K K I E T D I E P V M L H N Q V L V L K I R V F Q Q D T A Query_1 712 AAAAAGATTGAAACCGATATTGAGCCTGTGATGTTGCACAATCA-GGTGTTGGTATTAAAAATTAGAGTATTTCAACAAGATACCGCCAA 800 2 511 ............................................-............................................. 599 1 645 .......AN....NAN........A....................................N........ 576 N E I K R L I E E N S S S R L K A V L I D L R N N P G G L L Query_1 801 TGAGATTAAGCGTTTAATTGAAGAAAATAGTTCTTCGCGTTTAAAAGCGGTGTTAATTGATTTAAGAAATAACCCAGGCGGCTTGTTATC 890 2 600 .......................................................................................... 689 1 575 ..................N..............................................................N.N.....N 486 S A A V E S A D L F L N H G I I V S T K S R S E G N Q Q F Q Query_1 891 TGCTGCTGTTGAGTCAGCCGATTTATTTTTAAATCATGGCATTATTGTCTCTACAAAAAGTCGTTCAGAAGGCAATCAGCAATTTCAGGC 980 2 690 .......................................................................................... 779 1 485 .......................................................................................... 396 A L P G N D F Q N I K V G I L I N H R S A S A A E V F T A A Query_1 981 TTTGCCAGGTAACGATTTTCAAAATATAAAAGTTGGTATTTTAATTAATCACCGATCTGCCTCGGCAGCAGAAGTTTTTACTGCTGCAAT 1070 2 780 .......................................................................................... 869 1 395 ........................................................N.......................N......... 306 M K E H Q R A W V M G E K S Y G K G V V Q K L F P L P S G A Query_1 1071 GAAAGAGCATCAACGTGCTTGGGTCATGGGTGAAAAAAGTTATGGAAAGGGTGTTGTGCAAAAGCTTTTCCCTCTACCAAGTGGGGCAGC 1160 2 870 .......................................................................................... 959 1 305 ...................N...................................................................... 216 A L Q M T V S H Y Y T P N G N M I E G Q G I Q P N Q T Y P L Query_1 1161 GCTACAAATGACAGTCTCACATTACTACACACCAAATGGTAATATGATTGAGGGGCAAGGTATTCAGCCTAACCAGACTTATCCCTTACC 1250 2 960 .......................................................................................... 1049 1 215 ....................................................................N..................... 126 P P E M K E E V Y L D R V A D L L L K R K * H W K W I T D P Query_1 1251 CCCAGAAATGAAAGAAGAAGTTTATCTAGATCGTGTTGCCGATCTCCTTCTAAAAAGAAAGTAACATTGGAAGTGGATAACGGATCCGAA 1340 2 1050 ............................................................. 1110 1 125 .........................N...........T..TT.A.....................................--..... 40 N S S S V D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 1341 TTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCC--GAAAGGAA 1428 1 44 .....................CGA....... 14 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3045000 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 2,075 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96090.103 (IDP96090.1-504.F..pMCSG73)
MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGAGKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAENIYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALARNAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKDGRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPISLAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMMLCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPGAEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGVAVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA
ATGCAACAGTCTACCCCGTATCTCTCATTTCGCGGCATCGGTAAAACGTTTCCCGGCGTTAAGGCGCTGACGGATATTAGTTTTGACTGCTATGCCGGTCAGGTTCATGCGTTGATGGGTGAAAATGGCGCAGGAAAATCAACTCTCTTAAAAATCCTCAGCGGCAACTATGCGCCAACCACGGGTTCTGTAGTGATTAATGGGCAGGAAATGTCCTTTTCCGACACGACCGCAGCACTTAACGCGGGCGTGGCGATTATTTACCAGGAACTGCATCTCGTGCCGGAAATGACCGTCGCGGAAAACATCTATCTCGGCCAGCTGCCGCATAAAGGCGGCATTGTGAATCGCTCATTGCTGAATTATGAGGCGGGTTTACAACTTAAACATCTTGGTATGGATATTGACCCGGACACGCCGCTGAAATATCTCTCCATTGGTCAGTGGCAGATGGTTGAAATCGCCAAAGCGCTGGCGCGTAACGCCAAAATTATCGCCTTTGATGAGCCAACCAGCTCCCTCTCTGCCCGTGAAATCGACAATCTTTTCCGCGTTATTCGTGAACTGCGAAAAGAGGGGCGGGTAATCTTATACGTTTCTCACCGTATGGAAGAAATATTTGCCCTCAGCGATGCCATTACTGTCTTTAAAGATGGACGTTATGTCAAAACCTTTACCGATATGCAGCAGGTTGACCACGACGCGCTGGTGCAGGCGATGGTCGGGCGCGACATTGGCGATATCTACGGCTGGCAACCGCGTAGTTATGGCGAGGAGCGCCTACGTCTTGATGCTGTGAAAGCACCAGGCGTGCGTACGCCAATAAGTCTGGCGGTTCGCAGTGGTGAAATTGTTGGGCTGTTTGGTCTGGTAGGGGCGGGGCGTAGCGAATTAATGAAAGGCATGTTTGGCGGGACGCAAATCACCGCCGGTCAGGTTTATATCGACCAACAGCCGATCGATATTCGTAAACCGAGCCACGCCATTGCCGCAGGCATGATGCTCTGCCCGGAAGATCGCAAAGCGGAAGGCATTATTCCCGTGCACTCCGTTCGCGACAATATCAACATCAGTGCCAGACGTAAACATGTGCTCGGCGGTTGTGTAATCAACAACGGTTGGGAAGAAAACAATGCCGATCACCACATTCGTTCGCTCAACATCAAAACGCCGGGCGCGGAGCAACTGATCATGAATCTCTCAGGCGGAAATCAGCAAAAAGCCATTCTGGGCCGCTGGTTATCGGAAGAGATGAAGGTCATTTTGCTGGATGAACCTACGCGCGGCATTGATGTTGGCGCTAAGCACGAAATATATAACGTAATTTATGCGCTGGCGGCGCAGGGCGTGGCGGTGCTGTTTGCCTCCAGCGACTTACCTGAAGTCCTCGGCGTTGCCGACCGGATTGTGGTGATGCGGGAAGGTGAAATCGCCGGTGAATTGTTACACGAGCAGGCAGATGAGCGTCAGGCACTGAGCCTTGCGATGCCTAAAGTCAGCCAGGCTGTTGCC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,693 total letters Query= IDP96090.103 Length=1917 Score E Sequences producing significant alignments: (Bits) Value wt 2727 0.0 02F_F8_IDP96090.103_v73F.seq 1802 0.0 06F_F8_IDP96090.103_T7term.seq 1535 0.0 W S H P Q F E K G T E N L Y F Q S N A M Q Q S T P Y L S F R Query_1 145 TGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGCAACAGTCTACCCCGTATCTCTCATTTCGC 234 2 1 ................................. 33 0 41 ...........A........G....------........................................................... 124 G I G K T F P G V K A L T D I S F D C Y A G Q V H A L M G E Query_1 235 GGCATCGGTAAAACGTTTCCCGGCGTTAAGGCGCTGACGGATATTAGTTTTGACTGCTATGCCGGTCAGGTTCATGCGTTGATGGGTGAA 324 2 34 .......................................................................................... 123 2 928 ............T.. 942 0 125 .......................................................................................... 214 0 1017 ............T.. 1031 N G A G K S T L L K I L S G N Y A P T T G S V V I N G Q E M Query_1 325 AATGGCGCAGGAAAATCAACTCTCTTAAAAATCCTCAGCGGCAACTATGCGCCAACCACGGGTTCTGTAGTGATTAATGGGCAGGAAATG 414 2 124 .......................................................................................... 213 0 215 .......................................................................................... 304 S F S D T T A A L N A G V A I I Y Q E L H L V P E M T V A E Query_1 415 TCCTTTTCCGACACGACCGCAGCACTTAACGCGGGCGTGGCGATTATTTACCAGGAACTGCATCTCGTGCCGGAAATGACCGTCGCGGAA 504 2 214 .......................................................................................... 303 0 305 .......................................................................................... 394 N I Y L G Q L P H K G G I V N R S L L N Y E A G L Q L K H L Query_1 505 AACATCTATCTCGGCCAGCTGCCGCATAAAGGCGGCATTGTGAATCGCTCATTGCTGAATTATGAGGCGGGTTTACAACTTAAACATCTT 594 2 304 .......................................................................................... 393 0 395 .......................................................................................... 484 G M D I D P D T P L K Y L S I G Q W Q M V E I A K A L A R N Query_1 595 GGTATGGATATTGACCCGGACACGCCGCTGAAATATCTCTCCATTGGTCAGTGGCAGATGGTTGAAATCGCCAAAGCGCTGGCGCGTAAC 684 2 394 .......................................................................................... 483 0 485 .......................................................................................... 574 A K I I A F D E P T S S L S A R E I D N L F R V I R E L R K Query_1 685 GCCAAAATTATCGCCTTTGATGAGCCAACCAGCTCCCTCTCTGCCCGTGAAATCGACAATCTTTTCCGCGTTATTCGTGAACTGCGAAAA 774 2 484 .......................................................................................... 573 0 575 .......................................................................................... 664 E G R V I L Y V S H R M E E I F A L S D A I T V F K D G R Query_1 775 GAGGGGCGGGTAATCTTATACGTTTCTCACCGTATGGAAGAAATATTTGCCCTCAGCGATGCCATTACTGTC-TTTAAAGATGGACGTTA 863 2 574 ........................................................................-................. 662 0 665 ........................................................................T................. 754 1 920 .. 919 Y V K T F T D M Q Q V D H D A L V Q A M V G R D I G D I Y G Query_1 864 TGTCAAAACCTTTACCGATATGCAGCAGGTTGACCACGACGCGCT-GGTGCAGGCGATGGTCGGGCGCGACATTGGCGATATCTACGGCT 952 2 663 .............................................-............................................ 751 0 755 .............................................-............................................ 843 1 918 .......C.N..........N..........TGA...........T............................................ 829 W Q P R S Y G E E R L R L D A V K A P G V R T P I S L A V R Query_1 953 GGCAACCGCGTAGTTATGGCGAGGAGCGCCTACGTCTTGATGCTGTGAAAGCACCAGGCGTGCGTACGCCAA-TAAGTCTGGCGGTTCGC 1041 2 752 ........................................................................-................. 840 0 844 .................................................................N....N.T................. 933 1 828 ........................................................................-................. 740 S G E I V G L F G L V G A G R S E L M K G M F G G T Q I T A Query_1 1042 AGTGGTGAAATTGTTGGGCTGTTTGGTCTGGTAGGGGCGGGGCGTAGCGAATTAATGAAAGGCATGTTTGGCGGGACGCAAATCACCGCC 1131 2 841 .......................................................................................... 930 2 94 ... 96 0 934 .........T.GT......................T..-.......-.......N..-..............-........T..N..... 1019 0 185 ... 187 1 739 .......................................................................................... 650 G Q V Y I D Q Q P I D I R K P S H A I A A G M M L C P E Query_1 1132 GGTCAGG-TTTATATCGACCAACAGCCGATCGATATTCGTAAACCGAG-CCACGCCATTGCCG-CAGGCA-TGATGCT--CTGCCCGGAA 1215 2 931 .......-........................................-..............-......-.......--.......... 1014 2 97 .......-..C.. 108 0 1020 .......-..........................N.........N.N.C..N..T........C......T.......TCN......... 1108 0 188 .......-..C.. 199 1 649 .......G........................................-..............-......-.......--.......... 565 D R K A E G I I P V H S V R D N I N I S A R R K H V Query_1 1216 GA--TCGCAAAGCGGAAGGCA-TTATTCCCGTGC--ACTCCGTTCGC-GACAATATCAACATCA-GTG-CCAGA-CGTAAACATGT-GCT 1295 2 1015 ..--.................-............--...........-................-...-.....-...........-... 1094 0 1109 ..AT...N..GCT........N.....T...N..CAC....NN.NN.G...C.......N..N.T...C.....N.......NN..G.N. 1198 1 564 ..--.................-............--...........-................-...-.....-...........-... 485 L G G C V I N N G W E E N N A D H H I R S L N I K T P G A E Query_1 1296 CGGCGGTTGTGTAATCAACAACGGTTGGGAAGAAAACAATGCCGATCACCACATTCGTTCGCTCAACATCAAAACGCCGGGCGCGGAGCA 1385 2 1095 .......................................................................................... 1184 0 1199 .N........ 1208 1 484 .......................................................................................... 395 Q L I M N L S G G N Q Q K A I L G R W L S E E M K V I L L D Query_1 1386 ACTGATCATGAATCTCTCAGGCGGAAATCAGCAAAAAGCCATTCTGGGCCGCTGGTTATCGGAAGAGATGAAGGTCATTTTGCTGGATGA 1475 2 1185 .......................................................................................... 1274 1 394 .......................................................................................... 305 E P T R G I D V G A K H E I Y N V I Y A L A A Q G V A V L F Query_1 1476 ACCTACGCGCGGCATTGATGTTGGCGCTAAGCACGAAATATATAACGTAATTTATGCGCTGGCGGCGCAGGGCGTGGCGGTGCTGTTTGC 1565 2 1275 .......................................................................................... 1364 1 304 .......................................................................................... 215 A S S D L P E V L G V A D R I V V M R E G E I A G E L L H E Query_1 1566 CTCCAGCGACTTACCTGAAGTCCTCGGCGTTGCCGACCGGATTGTGGTGATGCGGGAAGGTGAAATCGCCGGTGAATTGTTACACGAGCA 1655 2 1365 .......................................................................................... 1454 0 937 ........... 947 1 214 .......................................................................................... 125 Q A D E R Q A L S L A M P K V S Q A V A * H W K W I T D P N Query_1 1656 GGCAGATGAGCGTCAGGCACTGAGCCTTGCGATGCCTAAAGTCAGCCAGGCTGTTGCCTAACATTGGAAGTGGATAACGGATCCGAATTC 1745 2 1455 .......................................................... 1512 1 124 ..............................................................................--..... 42 S S S V D K L A A A L E H H H H H H * D P A A N K A R Query_1 1746 GAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAA 1826 1 46 ......................... 22 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 6919800 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 3,693 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96111.104 (IDP96111.1-809.F..pMCSG73)
MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAHPGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEGVLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLDNDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLFLPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAETSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLHLIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRLLQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMGNSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDGRYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALLVDIDELVQRCPQLVQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVLNWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLDIYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATNPTIAQGRVPLVLELLSPAQRPLQITRDLSDFWKGAYREVQKEMKGRYPKHVWPDDPANTAPTRRTKKYS
GTGTCGTCGTTGCCCGTTGCTGCCGTCTTACCTGAATTACTTACCGCTCTCGATTGTGCGCCGCAGGTATTATTAAGTGCGCCGACCGGGGCCGGGAAATCAACCTGGCTGCCGCTGCAACTGCTGGCGCATCCCGGCATTAACGGGAAAATTATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTACCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAGCGGTGTTGGACTGGTGATCCTTGATGAATTTCATGAGCGCAGCTTGCAGGCGGATTTGGCGTTGGCGCTGTTACTCGATGTGCAACAAGGTCTGCGTGATGACCTTAAACTGCTGATTATGTCGGCTACCCTGGACAACGACCGCTTGCAGCAAATGCTGCCAGAAGCGCCTGTCGTCATCTCAGAAGGGCGCTCGTTTCCGGTTGAACGCCGTTATTTACCGCTGCCCGCGCATCAGCGTTTTGACGATGCCGTTGCGGTAGCCACCGCTGAAATGCTGCGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCAATGCTGGTTAGCGCGAAGAACGACGACGAAGCTGCTACCGCGGCAAAAATTGCCGCCATTCTCGAAGAGCCGCCACGGATGGGCAATAGTGACCTGGGCGTGGCGTTTTCGCGCAATCAACCAGCCTGGCAGCAACGTAGTCAGCAACTGTTAAAACGCTTAAACGTACGTGGCGGTGAGGCAGACAGTTCGCTTATCGCGCCGCTACTTGCCGGGGCGTTTGCCGATCGCATTGCTCGTCGCCGTGGGCAAGATGGACGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGGGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGACGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAGCGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGCCAGCGGTTGATGATGAAAGTTTATTGGCAGCGCTGGAAACGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,591 total letters Query= IDP96111.104 Length=2832 Score E Sequences producing significant alignments: (Bits) Value wt 4378 0.0 02G_G8_IDP96111.104_v73F.seq 919 0.0 06G_G8_IDP96111.104_T7term.seq 892 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 0 42 ...........A........G....------.............GA.---.CG. 86 0 26 ...C........... 40 0 27 .............. 40 A V S S L P V A A V L P E L L T A L D C A P Q V L L S A P T Query_1 199 GCCGTGTCGTCGTTGCCCGTTGCTGCCGTCTTACCTGAATTACTTACCGCTCTCGATTGTGCGCCGCAGGTATTATTAAGTGCGCCGACC 288 2 1 ....................................................................................... 87 0 87 ...................................N................................N..................... 176 G A G K S T W L P L Q L L A H P G I N G K I I L L E P R R L Query_1 289 GGGGCCGGGAAATCAACCTGGCTGCCGCTGCAACTGCTGGCGCATCCCGGCATTAACGGGAAAATTATCCTGCTGGAGCCGCGTCGTCTG 378 2 88 .......................................................................................... 177 0 177 .....................N...............................................................N.... 266 A A R N V A Q R L A E L L N E K P G D T V G Y R M R A Q N C Query_1 379 GCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTACCGGATGCGTGCGCAAAACTGC 468 2 178 .......................................................................................... 267 0 267 .....N.........N.......................................................................... 356 V G P N T R L E V V T E G V L T R M I Q R D P E L S G V G L Query_1 469 GTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAGCGGTGTTGGACTG 558 2 268 .......................................................................................... 357 0 357 ...........................................................N.............................. 446 V I L D E F H E R S L Q A D L A L A L L L D V Q Q G L R D D Query_1 559 GTGATCCTTGATGAATTTCATGAGCGCAGCTTGCAGGCGGATTTGGCGTTGGCGCTGTTACTCGATGTGCAACAAGGTCTGCGTGATGAC 648 2 358 .......................................................................................... 447 0 447 ............................N......N..............................................N....... 536 L K L L I M S A T L D N D R L Q Q M L P E A P V V I S E G R Query_1 649 CTTAAACTGCTGATTATGTCGGCTACCCTGGACAACGACCGCTTGCAGCAAATGCTGCCAGAAGCGCCTGTCGTCATCTCAGAAGGGCGC 738 2 448 .......................................................................................... 537 0 537 ................................................N................ 601 S F P V E R R Y L P L P A H Q R F D D A V A V A T A E M L R Query_1 739 TCGTTTCCGGTTGAACGCCGTTATTTACCGCTGCCCGCGCATCAGCGTTTTGACGATGCCGTTGCGGTAGCCACCGCTGAAATGCTGCGT 828 2 538 .......................................................................................... 627 Q E S G S L L L F L P G V G E I Q R V Q E Q L A S R I G S D Query_1 829 CAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTGGCTTCGCGCATCGGCAGTGAT 918 2 628 .......................................................................................... 717 2 1751 ........... 1761 V L L C P L Y G A L S L N D Q R K A I L P A P Q G M R K V V Query_1 919 GTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTG 1008 2 718 .......................................................................................... 807 2 1920 ........... 1930 L A T N I A E T S L T I E G I R L V V D C A Q E R V A R F D Query_1 1009 CTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGAT 1098 2 808 .......................................................................................... 897 P R T G L T R L I T Q R V S Q A S M T Q R A G R A G R L E P Query_1 1099 CCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCG 1188 2 898 .......................................................................................... 987 G I S L H L I A K E Q A E R A A A Q S E P E I L Q S D L S G Query_1 1189 GGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGT 1278 2 988 .......................................................................................... 1077 2 1039 ............ 1028 L L M E L L Q W G C S D P A Q M S W L D Q P P V V N L L A A Query_1 1279 TTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCG 1368 2 1078 .......................................................................................... 1167 K R L L Q M L G A L E G E R L S A Q G Q K M A A L G N D P R Query_1 1369 AAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGT 1458 2 1168 .......................................................................................... 1257 2 1973 ............ 1984 1 518 ............ 507 L A A M L V S A K N D D E A A T A A K I A A I L E E P P R M Query_1 1459 TTAGCGGCAATGCTGGTTAGCGCGAAGAACGACGACGAAGCTGCTACCGCGGCAAAAATTGCCGCCATTCTCGAAGAGCCGCCACGGATG 1548 2 1258 .......................................................................................... 1347 G N S D L G V A F S R N Q P A W Q Q R S Q Q L L K R L N V R Query_1 1549 GGCAATAGTGACCTGGGCGTGGCGTTTTCGCGCAATCAACCAGCCTGGCAGCAACGTAGTCAGCAACTGTTAAAACGCTTAAACGTACGT 1638 2 1348 .......................................................................................... 1437 G G E A D S S L I A P L L A G A F A D R I A R R R G Q D G R Query_1 1639 GGCGGTGAGGCAGACAGTTCGCTTATCGCGCCGCTACTTGCCGGGGCGTTTGCCGATCGCATTGCTCGTCGCCGTGGGCAAGATGGACGC 1728 2 1438 .......................................................................................... 1527 Y Q L A N G M G A M L D A N D A L S R H E W L I A P L L L Q Query_1 1729 TATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAG 1818 2 1528 .......................................................................................... 1617 G S A S P D A R I L L A L L V D I D E L V Q R C P Q L V Q Q Query_1 1819 GGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAG 1908 2 1618 .......................................................................................... 1707 S D T V E W D D A Q G T L K A W R R L Q I G Q L T V K V Q P Query_1 1909 TCTGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCG 1998 2 1708 .......................................................................................... 1797 2 658 ........... 668 L A K P S E D E L H Q A M L N G I R D K G L S V L N W T A E Query_1 1999 CTGGCGAAACCGTCAGAAGACGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAA 2088 2 1798 .......................................................................................... 1887 A E Q L R L R L L C A A K W L P E Y D W P A V D D E S L L A Query_1 2089 GCGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGCCAGCGGTTGATGATGAAAGTTTATTGGCA 2178 2 1888 .......................................................................................... 1977 2 1232 ..... 1236 2 796 ........... 806 2 2003 ........... 2013 1 563 .................................................. 514 A L E T W L L P H M T G V H S L R G L K S L D I Y Q A L R G Query_1 2179 GCGCTGGAAACGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGA 2268 2 1978 .......................................................................................... 2067 2 1237 ....... 1243 2 1938 ........... 1948 1 513 ................................C....NG..........................N...............N........ 424 1 553 ........... 543 L L D W G M Q Q R L D S E L P A H Y T V P T G S R I A I R Y Query_1 2269 TTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTAT 2358 2 2068 .......................................................................................... 2157 1 423 .....N.................................................................................... 334 H E D N P P A L A V R M Q E M F G E A T N P T I A Q G R V P Query_1 2359 CATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCG 2448 2 2158 .......................................................................................... 2247 1 333 ..................................N...G...........T...................................N..N 244 L V L E L L S P A Q R P L Q I T R D L S D F W K G A Y R E V Query_1 2449 CTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTG 2538 2 2248 .......................................................................................... 2337 1 243 .......................................................................................... 154 Q K E M K G R Y P K H V W P D D P A N T A P T R R T K K Y S Query_1 2539 CAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCG 2628 2 2338 .......................................................................................... 2427 1 153 .....................................................................N.................... 64 * H W K W I T D P N S S S V D K L A A A L E H H H H H H * D Query_1 2629 TAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGAT 2718 1 63 ....................--..... 39 1 43 .. 42 P A A N K A R K E A E L A A A T A Query_1 2719 CCGGCTGCTAAC-AAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGA 2771 2 1990 ............. 2002 2 607 ........... 617 1 41 ............A...........A... 14 1 501 ............. 489 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 9965100 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 3,591 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96135.103 (IDP96135.1-516.F..pMCSG73)
MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQLPAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLLTILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHANIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQMRFSQ
ATGCCCACTTCTCATGAAAATGCACTGCAACAACGTTGCCAGCAAATTGTCACCAGCCCAGTGCTTAGCCCGGAGCAGAAGCGCCATTTTCTGGCACTGGAAGCAGAAAACAATCTGCCTTACCCACAGCTTCCTGCCGAAGCCCGCCGCGCGCTGGATGAAGGTGTAATCTGCGATATGTTTGAAGGTCATGCGCCGTACAAACCGCGCTATGTCTTACCCGATTACGCCCGTTTTCTGGCGAACGGTTCCGAATGGCTGGAGCTGGAAGGCGCGAAAGATCTTGATGACGCACTCTCTCTGCTGACCATTCTTTACCACCACGTACCGTCGGTCACATCGATGCCGGTCTACCTGGGGCAACTGGATGCGTTGTTGCAACCGTATGTTAGAATTCTAACACAAGACGAGATCGATGTTCGAATAAAACGTTTCTGGCGTTACCTCGACAGAACCCTGCCAGACGCCTTTATGCACGCCAATATCGGCCCGTCTGATTCGCCCATTACCCGCGCGATCTTACGTGCAGATGCAGAGTTGAAGCAGGTTTCACCGAACCTGACCTTTATCTACGATCCTGAAATCACCCCTGATGACCTGCTGCTGGAAGTGGCGAAGAACATCTGTGAATGTAGCAAACCGCACATCGCCAACGGTCCGGTGCATGATAAAATTTTCACAAAAGGGGGCTACGGGATTGTGAGCTGTTACAACTCACTGCCGCTGGCGGGTGGTGGCAGCACGCTGGTACGCCTTAACCTGAAAGCCATTGCCGAGCGCAGCGAATCGCTGGATGACTTCTTTACGCGCACTCTACCGCACTACTGCCAGCAGCAGATCGCCATCATCGATGCGCGGTGTGAATTCCTCTATCAACAATCACACTTCTTTGAGAATAGCTTCCTGGTGAAAGAAGGGCTGATTAACCCTGAACGTTTTGTGCCAATGTTTGGCATGTATGGGCTGGCGGAAGCGGTTAACTTGCTGTGTGAAAAAGAAGGGATTGCCGCGCGCTACGGTAAAGAAGCCGCCGCAAATGAAGTAGGTTATCGCATCAGCGCGCAACTGGCGGAGTTTGTCGCCAATACCCCCGTGAAATATGGCTGGCAAAAACGCGCCATGTTACACGCACAGTCGGGGATCAGTTCCGATATCGGCACCACGCCGGGCGCGCGTTTGCCGTATGGCGATGAGCCAGATCCGATCACCCATCTGCAAACTGTCGCCCCGCATCATGCTTATTATTATTCCGGCATCAGCGACATTCTGACGCTCGACGAAACCATCAAACGTAACCCGCAGGCACTGGTACAGCTTTGCCTCGGTGCCTTTAAAGCCGGAATGCGTGAATTTACCGCCAATGTCAGCGGTAACGATCTGGTTCGCGTTACCGGTTATATGGTGCGTTTGTCGGATTTAGAAAAATATCGCGCCGAAGGTTCACGCACCAACACCACCTGGCTGGGCGAAGAAGCCGCACGCAACACTCGTATTCTGGAACGCCAGCCGCGCGTGATAAGCCATGAACAGCAGATGCGCTTTAGTCAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,326 total letters Query= IDP96135.103 Length=1953 Score E Sequences producing significant alignments: (Bits) Value wt 2792 0.0 02H_H8_IDP96135.103_v73F.seq 1878 0.0 06H_H8_IDP96135.103_T7term.seq 827 0.0 H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 111 CCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGC 200 0 42 ...........A........G....------......................... 91 0 29 ............ 40 0 30 ........... 40 A M P T S H E N A L Q Q R C Q Q I V T S P V L S P E Q K R H Query_1 201 CATGCCCACTTCTCATGAAAATGCACTGCAACAACGTTGCCAGCAAATTGTCACCAGCCCAGTGCTTAGCCCGGAGCAGAAGCGCCATTT 290 2 1 ......................................................................................... 89 0 92 .......................................................................................... 181 F L A L E A E N N L P Y P Q L P A E A R R A L D E G V I C D Query_1 291 TCTGGCACTGGAAGCAGAAAACAATCTGCCTTACCCACAGCTTCCTGCCGAAGCCCGCCGCGCGCTGGATGAAGGTGTAATCTGCGATAT 380 2 90 .......................................................................................... 179 0 182 .......................................................................................... 271 M F E G H A P Y K P R Y V L P D Y A R F L A N G S E W L E L Query_1 381 GTTTGAAGGTCATGCGCCGTACAAACCGCGCTATGTCTTACCCGATTACGCCCGTTTTCTGGCGAACGGTTCCGAATGGCTGGAGCTGGA 470 2 180 .......................................................................................... 269 0 272 .......................................................................................... 361 E G A K D L D D A L S L L T I L Y H H V P S V T S M P V Y L Query_1 471 AGGCGCGAAAGATCTTGATGACGCACTCTCTCTGCTGACCATTCTTTACCACCACGTACCGTCGGTCACATCGATGCCGGTCTACCTGGG 560 2 270 .......................................................................................... 359 0 362 .......................................................................................... 451 G Q L D A L L Q P Y V R I L T Q D E I D V R I K R F W R Y L Query_1 561 GCAACTGGATGCGTTGTTGCAACCGTATGTTAGAATTCTAACACAAGACGAGATCGATGTTCGAATAAAACGTTTCTGGCGTTACCTCGA 650 2 360 .......................................................................................... 449 2 402 ................ 387 0 452 .......................................................................................... 541 0 494 ................ 479 D R T L P D A F M H A N I G P S D S P I T R A I L R A D A E Query_1 651 CAGAACCCTGCCAGACGCCTTTATGCACGCCAATATCGGCCCGTCTGATTCGCCCATTACCCGCGCGATCTTACGTGCAGATGCAGAGTT 740 2 450 .......................................................................................... 539 0 542 .......................................................................................... 631 L K Q V S P N L T F I Y D P E I T P D D L L L E V A K N I C Query_1 741 GAAGCAGGTTTCACCGAACCTGACCTTTATCTACGATCCTGAAATCACCCCTGATGACCTGCTGCTGGAAGTGGCGAAGAACATCTGTGA 830 2 540 .......................................................................................... 629 0 632 .......................................................................................... 721 E C S K P H I A N G P V H D K I F T K G G Y G I V S C Y N S Query_1 831 ATGTAGCAAACCGCACATCGCCAACGGTCCGGTGCATGATAAAATTTTCACAAAAGGGGGCTACGGGATTGTGAGCTGTTACAACTCACT 920 2 630 .......................................................................................... 719 0 722 .......................................................................................... 811 L P L A G G G S T L V R L N L K A I A E R S E S L D D F F T Query_1 921 GCCGCTGGCGGGTGGTGGCAGCACGCTGGTACGCCTTAACCTGAAAGCCATTGCCGAGCGCAGCGAATCGCTGGATGACTTCTTTACGCG 1010 2 720 .......................................................................................... 809 0 812 .......................................................................................... 901 R T L P H Y C Q Q Q I A I I D A R C E F L Y Q Q S H F F E N Query_1 1011 CACTCTACCGCACTACTGCCAGCAGCAGATCGCCATCATCGATGCGCGGTG-TGAATTCCTCTATCAACAATCACACTTCTTTGAGAATA 1099 2 810 ...................................................-...................................... 898 0 902 ...................................................T...................................... 991 S F L V K E G L I N P E R F V P M F G M Y G L A E A V N L Query_1 1100 GCTTCCTGGTGAAAGAAGGGCTGATTAACCCTGAACGTTTTG-TGCCAATGTTTGGCATGTATGGG-CTGGCGGAAGCGGTTAA-CTTGC 1186 2 899 ..........................................-.......................-.................-..... 985 0 992 ...........................--.N.TG........T...........-.N.........C.....C...........C..... 1078 L C E K E G I A A R Y G K E A A A N E V G Y R I S A Q L Query_1 1187 TG-TGT-GAAAAAGAAGGGATTGC---CGCGCGCTACGGTAAAGAAGCCGCCGCAAATGAAGTAGGTTATCGCATCAGCGCGCAACTGGC 1271 2 986 ..-...-.................---............................................................... 1070 0 1079 ..NN..N........N..N..G..NNT...NGC....-......-....N......T..-................N......C...NNN 1165 A E F V A N T P V K Y G W Q K R A M L H A Q S G I S S D I G Query_1 1272 GGAGTTTGTCGCCAATACCCCCGTGAAA-TATGGCTGGCAAAAACGCGCCATGTTACACGCACAGTCGGGGATCAGTTCCGATATCGGCA 1360 2 1071 ............................-............................................................. 1159 0 1166 ................-......NN...-....... 1199 1 524 ........A............................................................. 455 T T P G A R L P Y G D E P D P I T H L Q T V A P H H A Y Y Y Query_1 1361 CCACGCCGGGCGCGCGTTTGCCGTATGGCGATGAGCCAGATCCGATCACCCATCTGCAAACTGTCGCCCCGCATCATGCTTATTATTATT 1450 2 1160 .......................................................................................... 1249 1 454 ..................................................................N....................... 365 S G I S D I L T L D E T I K R N P Q A L V Q L C L G A F K A Query_1 1451 CCGGCATCAGCGACATTCTGACGCTCGACGAAACCATCAAACGTAACCCGCAGGCACTGGTACAGCTTTGCCTCGGTGCCTTTAAAGCCG 1540 2 1250 .......................................................................................... 1339 1 364 ..........N......N.......................N................................................ 275 G M R E F T A N V S G N D L V R V T G Y M V R L S D L E K Y Query_1 1541 GAATGCGTGAATTTACCGCCAATGTCAGCGGTAACGATCTGGTTCGCGTTACCGGTTATATGGTGCGTTTGTCGGATTTAGAAAAATATC 1630 2 1340 .......................................................................................... 1429 1 274 ................N...........................N............................................. 185 R A E G S R T N T T W L G E E A A R N T R I L E R Q P R V I Query_1 1631 GCGCCGAAGGTTCACGCACCAACACCACCTGGCTGGGCGAAGAAGCCGCACGCAACACTCGTATTCTGGAACGCCAGCCGCGCGTGATAA 1720 2 1430 .......................................................................................... 1519 1 184 .......................................................................................... 95 S H E Q Q M R F S Q * H W K W I T D P N S S S V D K L A A A Query_1 1721 GCCATGAACAGCAGATGCGCTTTAGTCAGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCAC 1810 2 1520 ............................. 1548 1 94 .........T......N................................--..... 41 L E H H H H H H * D P A A N K A R Query_1 1811 TCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAA 1862 1 45 .............A......N.G.. 21 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 6349486 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 3,326 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96147.102 (IDP96147.1-481.F..pMCSG68)
MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLASALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTPGENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGVFDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMTTGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAKIAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQATLTSNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDVSWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFLDSGLLQECQQEHQQVTDTQPYHCPIPKNVTPSPLK
ATGCAGGAAATCTATCGTTTTATCGACGATGCGATTGAAGCCGATCGCCAACGTTATACCGATATTGCCGATCAAATCTGGGATCATCCAGAAACACGTTTTGAAGAGTTCTGGTCAGCGGAGCATCTGGCTTCGGCGCTGGAATCTGCAGGCTTCACCGTTACCCGCAACGTAGGCAATATCCCAAATGCCTTTATTGCTTCGTTTGGTCAAGGCAAACCGGTTATCGCCCTGCTGGGAGAATATGACGCCCTGGCAGGTTTAAGTCAGCAAGCAGGTTGCGCGCAACCTACATCCGTGACGCCCGGTGAAAATGGTCACGGTTGCGGACACAATTTGCTGGGAACCGCCGCCTTTGCCGCTGCAATAGCCGTCAAGAAATGGCTGGAACAATATGGGCAAGGCGGCACGGTGCGCTTTTATGGTTGTCCTGGCGAAGAAGGCGGCTCGGGTAAAACGTTCATGGTTCGCGAGGGGGTATTTGATGATGTGGATGCGGCACTCACCTGGCACCCGGAAGCCTTTGCCGGTATGTTCAATACCCGCACGCTGGCAAACATTCAGGCATCATGGCGCTTTAAAGGGATCGCAGCACATGCCGCGAATTCCCCTCATTTGGGACGCAGCGCCCTTGATGCCGTAACGTTGATGACCACTGGCACCAACTTCCTCAACGAACATATTATTGAAAAAGCGCGCGTACACTATGCCATCACAAATAGCGGCGGGATCTCGCCCAACGTGGTCCAGGCGCAGGCAGAAGTGCTTTATCTTATCCGCGCCCCCGAAATGACCGACGTGCAGCATATTTATGATCGGGTCGCCAAAATCGCCGAAGGTGCGGCATTGATGACCGAAACCACGGTTGAATGCCGCTTCGACAAAGCCTGTTCCAGTTATCTCCCGAATCGCACCTTAGAAAATGCCATGTACCAGGCCCTATCCCATTTTGGTACCCCGGAATGGAACTCCGAAGAACTGGCTTTTGCGAAACAAATTCAGGCTACGCTCACCTCCAACGATCGGCAAAACAGTCTGAATAATATCGCCGCAACCGGTGGCGAAAACGGCAAGGTTTTTGCACTACGTCATCGTGAAACGGTACTGGCGAATGAAGTCGCTCCATATGCCGCCACCGATAACGTGCTTGCGGCATCGACTGATGTCGGCGACGTCAGTTGGAAACTGCCTGTTGCCCAGTGTTTCAGCCCCTGTTTTGCCGTCGGTACACCGCTACATACGTGGCAACTGGTTAGCCAGGGGCGAACATCTATTGCTCATAAAGGAATGCTGCTGGCGGCGAAAACTATGGCAGCAACCACAGTCAATCTCTTCCTTGATTCAGGGCTATTGCAAGAATGCCAACAAGAGCATCAGCAAGTAACGGACACGCAACCGTATCACTGCCCTATCCCGAAAAACGTGACACCGTCACCTTTAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 2 sequences; 1,929 total letters Query= IDP96147.102 Length=1848 Score E Sequences producing significant alignments: (Bits) Value wt 2603 0.0 09G_C3_IDP96147.102_T7term.seq 764 0.0 M Q E I Y R F I D D A I E A D R Q R Y T D I A D Q I W D H P Query_1 202 ATGCAGGAAATCTATCGTTTTATCGACGATGCGATTGAAGCCGATCGCCAACGTTATACCGATATTGCCGATCAAATCTGGGATCATCCA 291 1 1 .......................................................................................... 90 E T R F E E F W S A E H L A S A L E S A G F T V T R N V G N Query_1 292 GAAACACGTTTTGAAGAGTTCTGGTCAGCGGAGCATCTGGCTTCGGCGCTGGAATCTGCAGGCTTCACCGTTACCCGCAACGTAGGCAAT 381 1 91 .......................................................................................... 180 I P N A F I A S F G Q G K P V I A L L G E Y D A L A G L S Q Query_1 382 ATCCCAAATGCCTTTATTGCTTCGTTTGGTCAAGGCAAACCGGTTATCGCCCTGCTGGGAGAATATGACGCCCTGGCAGGTTTAAGTCAG 471 1 181 .......................................................................................... 270 Q A G C A Q P T S V T P G E N G H G C G H N L L G T A A F A Query_1 472 CAAGCAGGTTGCGCGCAACCTACATCCGTGACGCCCGGTGAAAATGGTCACGGTTGCGGACACAATTTGCTGGGAACCGCCGCCTTTGCC 561 1 271 .......................................................................................... 360 1 291 ................ 276 A A I A V K K W L E Q Y G Q G G T V R F Y G C P G E E G G S Query_1 562 GCTGCAATAGCCGTCAAGAAATGGCTGGAACAATATGGGCAAGGCGGCACGGTGCGCTTTTATGGTTGTCCTGGCGAAGAAGGCGGCTCG 651 1 361 .......................................................................................... 450 G K T F M V R E G V F D D V D A A L T W H P E A F A G M F N Query_1 652 GGTAAAACGTTCATGGTTCGCGAGGGGGTATTTGATGATGTGGATGCGGCACTCACCTGGCACCCGGAAGCCTTTGCCGGTATGTTCAAT 741 1 451 .......................................................................................... 540 T R T L A N I Q A S W R F K G I A A H A A N S P H L G R S A Query_1 742 ACCCGCACGCTGGCAAACATTCAGGCATCATGGCGCTTTAAAGGGATCGCAGCACATGCCGCGAATTCCCCTCATTTGGGACGCAGCGCC 831 1 541 .......................................................................................... 630 L D A V T L M T T G T N F L N E H I I E K A R V H Y A I T N Query_1 832 CTTGATGCCGTAACGTTGATGACCACTGGCACCAACTTCCTCAACGAACATATTATTGAAAAAGCGCGCGTACACTATGCCATCACAAAT 921 1 631 .......................................................................................... 720 S G G I S P N V V Q A Q A E V L Y L I R A P E M T D V Q H I Query_1 922 AGCGGCGGGATCTCGCCCAACGTGGTCCAGGCGCAGGCAGAAGTGCTTTATCTTATCCGCGCCCCCGAAATGACCGACGTGCAGCATATT 1011 1 721 .......................................................................................... 810 Y D R V A K I A E G A A L M T E T T V E C R F D K A C S S Y Query_1 1012 TATGATCGGGTCGCCAAAATCGCCGAAGGTGCGGCATTGATGACCGAAACCACGGTTGAATGCCGCTTCGACAAAGCCTGTTCCAGTTAT 1101 1 811 .......................................................................................... 900 L P N R T L E N A M Y Q A L S H F G T P E W N S E E L A F A Query_1 1102 CTCCCGAATCGCACCTTAGAAAATGCCATGTACCAGGCCCTATCCCATTTTGGTACCCCGGAATGGAACTCCGAAGAACTGGCTTTTGCG 1191 1 901 .......................................................................................... 990 K Q I Q A T L T S N D R Q N S L N N I A A T G G E N G K V F Query_1 1192 AAACAAATTCAGGCTACGCTCACCTCCAACGATCGGCAAAACAGTCTGAATAATATCGCCGCAACCGGTGGCGAAAACGGCAAGGTTTTT 1281 1 991 .......................................................................................... 1080 1 1222 ........... 1212 0 353 ........... 363 A L R H R E T V L A N E V A P Y A A T D N V L A A S T D V G Query_1 1282 GCACTACGTCATCGTGAAACGGTACTGGCGAATGAAGTCGCTCCATATGCCGCCACCGATAACGTGCTTGCGGCATCGACTGATGTCGGC 1371 1 1081 .......................................................................................... 1170 0 486 ...........N...................................N...............A....N...A......... 405 D V S W K L P V A Q C F S P C F A V G T P L H T W Q L V S Q Query_1 1372 GACGTCAGTTGGAAACTGCCTGTTGCCCAGTGTTTCAGCCCCTGTTTTGCCGTCGGTACACCGCTACATACGTGGCAACTGGTTAGCCAG 1461 1 1171 .......................................................................................... 1260 1 1034 ........... 1024 0 404 ..........................N............................................................... 315 G R T S I A H K G M L L A A K T M A A T T V N L F L D S G L Query_1 1462 GGGCGAACATCTATTGCTCATAAAGGAATGCTGCTGGCGGCGAAAACTATGGCAGCAACCACAGTCAATCTCTTCCTTGATTCAGGGCTA 1551 1 1261 .......................................................................................... 1350 0 314 ..........N....................N........N.....N........................................... 225 L Q E C Q Q E H Q Q V T D T Q P Y H C P I P K N V T P S P L Query_1 1552 TTGCAAGAATGCCAACAAGAGCATCAGCAAGTAACGGACACGCAACCGTATCACTGCCCTATCCCGAAAAACGTGACACCGTCACCTTTA 1641 1 1351 .......................................................................................... 1440 0 224 .........................................................................N................ 135 K * H W K W I T D P N S S S V D K L A A A L E H H H H H H * Query_1 1642 AAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGA 1731 1 1441 ... 1443 0 134 ..............................................T.T.A......N...........---.................. 48 D P A A N K A R Query_1 1732 GATCCGGCTGCTAAC-AAAGCCCGAAA 1757 0 47 ...............A........... 21 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3475304 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 1,929 Number of sequences in database: 2 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96147.103 (IDP96147.1-481.F..pMCSG73)
MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLASALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTPGENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGVFDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMTTGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAKIAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQATLTSNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDVSWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFLDSGLLQECQQEHQQVTDTQPYHCPIPKNVTPSPLK
ATGCAGGAAATCTATCGTTTTATCGACGATGCGATTGAAGCCGATCGCCAACGTTATACCGATATTGCCGATCAAATCTGGGATCATCCAGAAACACGTTTTGAAGAGTTCTGGTCAGCGGAGCATCTGGCTTCGGCGCTGGAATCTGCAGGCTTCACCGTTACCCGCAACGTAGGCAATATCCCAAATGCCTTTATTGCTTCGTTTGGTCAAGGCAAACCGGTTATCGCCCTGCTGGGAGAATATGACGCCCTGGCAGGTTTAAGTCAGCAAGCAGGTTGCGCGCAACCTACATCCGTGACGCCCGGTGAAAATGGTCACGGTTGCGGACACAATTTGCTGGGAACCGCCGCCTTTGCCGCTGCAATAGCCGTCAAGAAATGGCTGGAACAATATGGGCAAGGCGGCACGGTGCGCTTTTATGGTTGTCCTGGCGAAGAAGGCGGCTCGGGTAAAACGTTCATGGTTCGCGAGGGGGTATTTGATGATGTGGATGCGGCACTCACCTGGCACCCGGAAGCCTTTGCCGGTATGTTCAATACCCGCACGCTGGCAAACATTCAGGCATCATGGCGCTTTAAAGGGATCGCAGCACATGCCGCGAATTCCCCTCATTTGGGACGCAGCGCCCTTGATGCCGTAACGTTGATGACCACTGGCACCAACTTCCTCAACGAACATATTATTGAAAAAGCGCGCGTACACTATGCCATCACAAATAGCGGCGGGATCTCGCCCAACGTGGTCCAGGCGCAGGCAGAAGTGCTTTATCTTATCCGCGCCCCCGAAATGACCGACGTGCAGCATATTTATGATCGGGTCGCCAAAATCGCCGAAGGTGCGGCATTGATGACCGAAACCACGGTTGAATGCCGCTTCGACAAAGCCTGTTCCAGTTATCTCCCGAATCGCACCTTAGAAAATGCCATGTACCAGGCCCTATCCCATTTTGGTACCCCGGAATGGAACTCCGAAGAACTGGCTTTTGCGAAACAAATTCAGGCTACGCTCACCTCCAACGATCGGCAAAACAGTCTGAATAATATCGCCGCAACCGGTGGCGAAAACGGCAAGGTTTTTGCACTACGTCATCGTGAAACGGTACTGGCGAATGAAGTCGCTCCATATGCCGCCACCGATAACGTGCTTGCGGCATCGACTGATGTCGGCGACGTCAGTTGGAAACTGCCTGTTGCCCAGTGTTTCAGCCCCTGTTTTGCCGTCGGTACACCGCTACATACGTGGCAACTGGTTAGCCAGGGGCGAACATCTATTGCTCATAAAGGAATGCTGCTGGCGGCGAAAACTATGGCAGCAACCACAGTCAATCTCTTCCTTGATTCAGGGCTATTGCAAGAATGCCAACAAGAGCATCAGCAAGTAACGGACACGCAACCGTATCACTGCCCTATCCCGAAAAACGTGACACCGTCACCTTTAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,185 total letters Query= IDP96147.103 Length=1848 Score E Sequences producing significant alignments: (Bits) Value wt 2603 0.0 07A_A9_IDP96147.103_T7term.seq 1763 0.0 03A_A9_IDP96147.103_v73F.seq 735 0.0 W S H P Q F E K G T E N L Y F Q S N A M Q E I Y R F I D D A Query_1 145 TGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGCAGGAAATCTATCGTTTTATCGACGATGCG 234 2 1 ................................. 33 0 44 .......N...A........G....------..................T.T...N.................................. 127 I E A D R Q R Y T D I A D Q I W D H P E T R F E E F W S A E Query_1 235 ATTGAAGCCGATCGCCAACGTTATACCGATATTGCCGATCAAATCTGGGATCATCCAGAAACACGTTTTGAAGAGTTCTGGTCAGCGGAG 324 2 34 .......................................................................................... 123 0 128 .........................................................N................N............... 217 H L A S A L E S A G F T V T R N V G N I P N A F I A S F G Q Query_1 325 CATCTGGCTTCGGCGCTGGAATCTGCAGGCTTCACCGTTACCCGCAACGTAGGCAATATCCCAAATGCCTTTATTGCTTCGTTTGGTCAA 414 2 124 .......................................................................................... 213 0 218 ...........T.......................................N...................................... 307 G K P V I A L L G E Y D A L A G L S Q Q A G C A Q P T S V T Query_1 415 GGCAAACCGGTTATCGCCCTGCTGGGAGAATATGACGCCCTGGCAGGTTTAAGTCAGCAAGCAGGTTGCGCGCAACCTACATCCGTGACG 504 2 214 .......................................................................................... 303 2 291 ................ 276 0 308 ...............................................................T.......................... 397 0 385 .............. 372 P G E N G H G C G H N L L G T A A F A A A I A V K K W L Query_1 505 CCCGGTGAAAATGGTCACGGTTGCGGACACAAT-TTGCTGGGAACCGCCGCCTTTG-CCGCTGCAA--TAGCCGTC-AAGAAAT-GGCTG 588 2 304 .................................-......................-.........--........-.......-..... 387 1 1205 ....A........GNN.....N.C.N.A.N.TN....AA....NN..CN.....NA..... 1145 0 398 .................................-......................-.........--........-.......-..... 481 E Q Y G Q G G T V R F Y G C P G E E G G S G K T F M Query_1 589 GAACAATAT---GGGCAAGGCGG--CACGGTG-CGCTTTT-ATGGTTGTCCTGG-CGAAGAA--GGCGGCTCGGGTAAAACGTT-CATGG 667 2 388 .........---...........--.......-.......-.............-.......--....................-..... 466 1 1144 A...C....ATA...........GC..N....N.......N.............A.......AG.NN.AT....TA........N..... 1055 0 482 ... 484 V R E G V F D D V D A A L T W H P E A F A G M F N T R T L Query_1 668 TTCGCGAGGGGG-TATTTGATGATGTGGATGCGGC-ACTCACCTGGCACCCGG-AAGCCTTTGCCGGTATGTTCAATACCCGCACGCTGG 754 2 467 ............-......................-.................-.................................... 553 1 1054 ............G......................N.N...............G........A........G..............N... 965 A N I Q A S W R F K G I A A H A A N S P H L G R S A L D A Query_1 755 CAAACATTCAGGCATCATGGCGCTTTAAAGGGATCGCAGCACATGCCGCGAA-TTCCCCTCATTTGGG-ACGCAGCGCCCTTG-ATGCCG 841 2 554 ....................................................-...............-..............-...... 640 1 964 ...........................................NN.......A...............N...........N..N...... 875 V T L M T T G T N F L N E H I I E K A R V H Y A I T N S G Query_1 842 TAACGTTGATGACCACTGGCACCAACTTCC--TCAACGAA-CATATTATTGAAAAAGCGCGCGTACACTATGCCATCACAAA-TAGCGGC 927 2 641 ..............................--........-.........................................-....... 726 1 874 ............................T.CT........A.........................................A....... 785 G I S P N V V Q A Q A E V L Y L I R A P E M T D V Q H I Y Query_1 928 GGGATCTCGCCCAACGTGGTCCAGGCGCAGGCAGAA-GTGCTTTATCTTATCCGCGCCCCCGAAATGACCGACGTGCAGCATATTTATGA 1016 2 727 ....................................-..................................................... 815 1 784 ....................................A..................................................... 695 D R V A K I A E G A A L M T E T T V E C R F D K A C S S Y L Query_1 1017 TCGGGTCGCCAAAATCGCCGAA-GGTGCGGCATTGATGACCGAAACCACGGTTGAATGCCGCTTCGACAAAGCCTGTTCCAGTTATCTCC 1105 2 816 ......................-................................................................... 904 1 694 ......................A................................................................... 605 P N R T L E N A M Y Q A L S H F G T P E W N S E E L A F A K Query_1 1106 CGAATCGCACCTTAGAAAATGCCATGTACCAGGCCCTATCCCATTTTGGTACCCCGGAATGGAACTCCGAAGAACTGGCTTTTGCGAAAC 1195 2 905 .......................................................................................... 994 1 604 .......................................................................................... 515 Q I Q A T L T S N D R Q N S L N N I A A T G G E N G K V F A Query_1 1196 AAATTCAGGCTACGCTCACCTCCAACGATCGGCAAAACAGTCTGAATAATATCGCCGCAACCGGTGGCGAAAACGGCAAGGTTTTTGCAC 1285 2 995 .......................................................................................... 1084 2 1222 ........... 1212 1 514 .......................................................................................... 425 1 287 ........... 297 L R H R E T V L A N E V A P Y A A T D N V L A A S T D V G D Query_1 1286 TACGTCATCGTGAAACGGTACTGGCGAATGAAGTCGCTCCATATGCCGCCACCGATAACGTGCTTGCGGCATCGACTGATGTCGGCGACG 1375 2 1085 .......................................................................................... 1174 1 424 .......................................................................................... 335 V S W K L P V A Q C F S P C F A V G T P L H T W Q L V S Q G Query_1 1376 TCAGTTGGAAACTGCCTGTTGCCCAGTGTTTCAGCCCCTGTTTTGCCGTCGGTACACCGCTACATACGTGGCAACTGGTTAGCCAGGGGC 1465 2 1175 .......................................................................................... 1264 2 1034 ........... 1024 1 334 .......................................................................................... 245 1 475 ........... 485 R T S I A H K G M L L A A K T M A A T T V N L F L D S G L L Query_1 1466 GAACATCTATTGCTCATAAAGGAATGCTGCTGGCGGCGAAAACTATGGCAGCAACCACAGTCAATCTCTTCCTTGATTCAGGGCTATTGC 1555 2 1265 .......................................................................................... 1354 1 244 .......................................................................................... 155 Q E C Q Q E H Q Q V T D T Q P Y H C P I P K N V T P S P L K Query_1 1556 AAGAATGCCAACAAGAGCATCAGCAAGTAACGGACACGCAACCGTATCACTGCCCTATCCCGAAAAACGTGACACCGTCACCTTTAAAAT 1645 2 1355 ......................................................................................... 1443 1 154 ...........................N.............................................................. 65 * H W K W I T D P N S S S V D K L A A A L E H H H H H H * D Query_1 1646 AACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATC 1735 1 64 ...................--..... 41 1 45 ... 43 P A A N K A R Query_1 1736 CGGCTGCTAACAAAGCCCGAAA 1757 1 42 ...................... 21 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5746984 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 19, 2017 10:10 PM Number of letters in database: 3,185 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2