Clone ID: APC113179.120 (APC113179.3-243.P(247).S191P.pMCSG68)
VDQYKKEIGMMNLEKLIQDREGISSKKFPIEDKELLFRYEQLYTGAVNDVMREFCLLEQALPGRIKPLREYHSVAGFAFTVKSAPNVKIKGEMEYRTQMLDEMQEDHFVVWDTSRDDKATLWGGVMTATAKGKKLKAACIDGGIRDTHQILNADFPVFYEYRISNGSLGRCLITHYQIPIKIGDVTIKPGDIILGDIDGVLVVPREIAYEVLLRSEEIRENEKKIFSWVQNGDSVQEITAK
GTAGATCAGTATAAGAAAGAAATCGGAATGATGAATCTAGAAAAATTGATTCAGGATCGTGAAGGAATATCTTCGAAAAAGTTTCCTATAGAGGATAAAGAATTATTATTTCGATACGAGCAGTTGTATACAGGGGCCGTAAACGATGTGATGCGTGAATTTTGCCTTTTGGAGCAAGCCTTACCCGGTAGGATTAAACCTTTACGCGAATATCATTCTGTTGCCGGTTTTGCCTTTACTGTCAAAAGTGCACCAAACGTAAAGATAAAGGGTGAGATGGAATATCGAACCCAGATGCTGGATGAGATGCAGGAAGACCATTTTGTTGTCTGGGATACAAGTCGGGATGATAAAGCTACTTTGTGGGGAGGTGTAATGACGGCAACTGCTAAAGGGAAGAAACTGAAAGCCGCTTGTATAGATGGGGGAATACGGGATACACATCAGATTTTGAATGCTGATTTTCCTGTGTTTTACGAATATCGTATATCGAATGGTAGCTTGGGGCGCTGTTTGATTACTCACTATCAGATTCCAATTAAAATCGGAGATGTAACGATTAAGCCGGGTGATATAATACTTGGAGATATCGATGGAGTTTTAGTTGTACCAAGGGAAATAGCTTATGAAGTGCTTTTACGCTCGGAGGAGATACGTGAAAATGAGAAGAAGATTTTCTCTTGGGTACAAAACGGAGACAGTGTTCAGGAAATTACAGCAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,409 total letters Query= APC113179.120 Length=1128 Score E Sequences producing significant alignments: (Bits) Value wt 1305 0.0 08G_G3_APC113179.120T7term.seq 1182 0.0 03G_G3_APC113179.120T7sh.seq 1182 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 59 TCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATT-CTTCTGGTGTAGATCTGTGG 147 1 4 .......-....-........N.......................................C.C.TT..N.NN..............N.. 91 S H P Q F E K G T E N L Y F Q S N A V D Q Y K K E I G M M N Query_1 148 TCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGTAGATCAGTATAAGAAAGAAATCGGAATGATGAAT 237 0 1 .................................... 36 1 92 ................CNTT...........................................N.......................... 181 L E K L I Q D R E G I S S K K F P I E D K E L L F Query_1 238 CTAGAAAAATTGA--TTCAGG-ATC-GTG-AAGGAA-TATCTTCGAAAAA--GTTT-CCT-ATAGAGG--ATAAA-GAATTATTATT-TC 313 0 37 .............--......-...-...-......-.............--....-...-.......--.....-...........-.. 112 2 826 .........AT......T...N...G......A.......NN....AT....N...N....GN.GN.N...N...........A.. 741 1 182 .............--......-...-...-......-.............--....-...-.......--.....-.......N...-.. 257 R Y E Q L Y T G A V N D V M R E F C L L E Q A L P G R I Query_1 314 GATA-CG-AGCA-GTTGTATACAGGGG-CCGTAAA-CGATGTGATGCGTGAATTTTGCCTTTTGGAGCAAGCCTTACCCGGTAGGATTAA 398 0 113 ....-..-....-..............-.......-...................................................... 197 2 740 ...NN..N...NN.....N........N.......A.....N............N.............N...NN.....N.A........ 651 1 258 ....-..-....-..............-.......-............N............N............................ 342 K P L R E Y H S V A G F A F T V K S A P N V K I K G E M E Y Query_1 399 ACCTTTACGCGAATATCATTC-TGTTGCCGGTTTTGCCTTTACTGTCAAAAGTGCACCAAACGTAAAGATAAAGGGTGAGA-TGGAATAT 486 0 198 .....................-...........................................................-........ 285 2 650 ....N.N..............C.........N..A.....NCN......................................A......N. 561 1 343 .....................-......................................................N....-........ 430 R T Q M L D E M Q E D H F V V W D T S R D D K A T L W G G V Query_1 487 CGAACCCAGATGCTGGATGAGATGCAGGAAGACCATTTTGTTGTCTGGGATACAAGTCGGGATGATAAAGCTACTTTGTGGGGAGGTGTA 576 0 286 .......................................................................................... 375 2 560 ...N...N.N..N..............N.............................................................. 471 1 431 ......................................N...........N...........................C........... 520 M T A T A K G K K L K A A C I D G G I R D T H Q I L N A D Query_1 577 ATGACGGCAACTGCTAAAGGGAAGAAACTGAAAGCCGCTTGTATAGATGGGGGAATACGGGATACACATCAGATTTTGAATGCTGATTT- 665 0 376 .........................................................................................- 464 2 470 .........................................................................................- 382 1 521 .........................................N..........................C.N..................C 610 F P V F Y E Y R I S N G S L G R C L I T H Y Q I P I K I G Query_1 666 TCCTGTGTTTTACGAATAT-CGTATATCGAATGGTAGCTT-GGGGCGCTGTTTGATTACTCACTATCAG-ATTCCAATTAAAATCGGAGA 752 0 465 ...................-....................-............................-.................... 551 2 381 ...................-....................-............................-.................... 295 1 611 .......N...........C....................G.........N..................N.N.....N.N.......... 700 D V T I K P G D I I L G D I D G V L V V P R E I A Y E Query_1 753 TGTAACGATTAAG-CCGGGTGATATAATA-CTTGGAG-ATATCGAT--GGAGTTTTAGTT-GTACCA-AGGG-AAATAGCTT-ATGAAGT 833 0 552 .............-...............-.......-........--............-......-....-.........-....... 632 2 294 .............-...............-.......-........--............-..N...-....-.........-....... 214 1 701 .............C.....N..C.N....C.......C...N.NGNCG....N..C....G.C....C....A...N.....A.N..... 790 V L L R S E E I R E N E K K I F S W V Q N G D S V Q Query_1 834 GCTTTTA-CGCTCGG--AGGAGATACGT-GAAAATGA-GAAGAAG-ATTTTCTCTT--GGGTACAA-AACGGA-GACAGTGTTCA--GGA 911 0 633 .......-.......--...........-........-.......-..........--........-......-...........--... 710 2 213 .......-.......--...........-........-.......-..........--........-......-...........--... 136 1 791 ......NC.C...N.NA....C.N....N.....N..CN......A.C........TG...N....N......NN..G...N...AG... 880 E I T A K * H W K W I T D P N S S S V D K L A A A L E H H H Query_1 912 AA-TTACAGCAAAATAACATTGGAAGTGGATAA-CGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccac 999 0 711 ..-........... 723 2 135 ..-..............................T................................................N....... 47 1 881 ..T....GC..... 894 H H H * D P A A N K A R K E Query_1 1000 cacca-ccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAG 1042 2 46 ....NC............N.......T........N..N..... 3 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3737432 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:02 AM Number of letters in database: 3,409 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC113179.121 (APC113179.3-247.P(247).S191P.pMCSG68)
VDQYKKEIGMMNLEKLIQDREGISSKKFPIEDKELLFRYEQLYTGAVNDVMREFCLLEQALPGRIKPLREYHSVAGFAFTVKSAPNVKIKGEMEYRTQMLDEMQEDHFVVWDTSRDDKATLWGGVMTATAKGKKLKAACIDGGIRDTHQILNADFPVFYEYRISNGSLGRCLITHYQIPIKIGDVTIKPGDIILGDIDGVLVVPREIAYEVLLRSEEIRENEKKIFSWVQNGDSVQEITAKGGYF
GTAGATCAGTATAAGAAAGAAATCGGAATGATGAATCTAGAAAAATTGATTCAGGATCGTGAAGGAATATCTTCGAAAAAGTTTCCTATAGAGGATAAAGAATTATTATTTCGATACGAGCAGTTGTATACAGGGGCCGTAAACGATGTGATGCGTGAATTTTGCCTTTTGGAGCAAGCCTTACCCGGTAGGATTAAACCTTTACGCGAATATCATTCTGTTGCCGGTTTTGCCTTTACTGTCAAAAGTGCACCAAACGTAAAGATAAAGGGTGAGATGGAATATCGAACCCAGATGCTGGATGAGATGCAGGAAGACCATTTTGTTGTCTGGGATACAAGTCGGGATGATAAAGCTACTTTGTGGGGAGGTGTAATGACGGCAACTGCTAAAGGGAAGAAACTGAAAGCCGCTTGTATAGATGGGGGAATACGGGATACACATCAGATTTTGAATGCTGATTTTCCTGTGTTTTACGAATATCGTATATCGAATGGTAGCTTGGGGCGCTGTTTGATTACTCACTATCAGATTCCAATTAAAATCGGAGATGTAACGATTAAGCCGGGTGATATAATACTTGGAGATATCGATGGAGTTTTAGTTGTACCAAGGGAAATAGCTTATGAAGTGCTTTTACGCTCGGAGGAGATACGTGAAAATGAGAAGAAGATTTTCTCTTGGGTACAAAACGGAGACAGTGTTCAGGAAATTACAGCAAAAGGTGGTTACTTT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,129 total letters Query= APC113179.121 Length=1140 Score E Sequences producing significant alignments: (Bits) Value wt 1326 0.0 03H_H3_APC113179.121T7sh.seq 230 2e-63 08H_H3_APC113179.121T7term.seq 212 4e-58 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 57 ATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTG 146 1 4 ...T....TCN....N.-.................................................N...................... 92 W S H P Q F E K G T E N L Y F Q S N A V D Q Y K K E I G M M Query_1 147 GTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGTAGATCAGTATAAGAAAGAAATCGGAATGATGAA 236 0 1 ................................... 35 1 93 ....................................................... 147 N L E K L I Q D R E G I S S K K F P I E D K E L L F R Y E Q Query_1 237 TCTAGAAAAATTGATTCAGGATCGTGAAGGAATATCTTCGAAAAAGTTTCCTATAGAGGATAAAGAATTATTATTTCGATACGAGCAGTT 326 0 36 .......................................................................................... 125 L Y T G A V N D V M R E F C L L E Q A L P G R I K P L R E Y Query_1 327 GTATACAGGGGCCGTAAACGATGTGATGCGTGAATTTTGCCTTTTGGAGCAAGCCTTACCCGGTAGGATTAAACCTTTACGCGAATATCA 416 0 126 .......................................................................................... 215 H S V A G F A F T V K S A P N V K I K G E M E Y R T Q M L D Query_1 417 TTCTGTTGCCGGTTTTG--CCTTTACTGTCAAAAGTGCACCAAACGTAAAGATAAAGGGTGAGATGGAATATCGAACCCAGATGCTGGAT 504 0 216 .................--....................................................................... 303 2 523 ...........AT...... 505 E M Q E D H F V V W D T S R D D K A T L W G G V M T A T A K Query_1 505 GAGATGCAGGAAGACCATTTTGTTGTCTGGGATACAAGTCGGGATGATAAAGCTACTTTGTGGGGAGGTGTAATGACGGCAACTGCTAAA 594 0 304 .......................................................................................... 393 G K K L K A A C I D G G I R D T H Q I L N A D F P V F Y E Y Query_1 595 GGGAAGAAACTGAAAGCCGCTTGTATAGATGGGGGAATACGGGATACACATCAGATTTTGAATGCTGATTTTCCTGTGTTTTACGAATAT 684 0 394 .......................................................................................... 483 1 460 ........... 450 R I S N G S L G R C L I T H Y Q I P I K I G D V T I K P G D Query_1 685 CGTATATCGAATGGTAGCTTGGGGCGCTGTTTGATTACTCACTATCAGATTCCAATTAAAATCGGAGATGTAACGATTAAGCCGGGTGAT 774 0 484 .......................................................................................... 573 I I L G D I D G V L V V P R E I A Y E V L L R S E E I R E N Query_1 775 ATAATACTTGGAGATATCGATGGAGTTTTAGTTGTACCAAGGGAAATAGCTTATGAAGTGCTTTTACGCTCGGAGGAGATACGTGAAAAT 864 0 574 .......................................................................................... 663 E K K I F S W V Q N G D S V Q E I T A K G G Y F * H W K W I Query_1 865 GAGAAGAAGATTTTCTCTTGGGTACAAAACGGAGACAGTGTTCAGGAAATTACAGCAAAAGGTGGTTACTTTTAACATTGGAAGTGGATA 954 0 664 ........................................................................ 735 1 234 ........... 224 2 124 ................... 106 T D P N S S S V D K L A A A L E H H H H H H * D P A A N K A Query_1 955 ACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCC 1044 2 105 .......................................................................................... 16 R K E Query_1 1045 CGAAAGGAAGC 1055 2 15 .....A..... 5 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3463044 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:02 AM Number of letters in database: 3,129 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC114117.102 (APC114117.1-254.F..pMCSG68)
MTREFIMLMVPPLTDGFPLDILVANVVACFLLGTVTALYARKIHSRDVHTIIGTGMMGGVSTFSSFAYGSVVLASASMSAFLIAAAYVTVSVVAGYVAVLAGMKFGEKSADILHRYPPMASIIDSGLVTVESRHSVAETIERVAAKAKSMGMNVFTRVDHGAGAKEAGLGLPPTELIIFGNPQNGTVLMQDKRTIGLDLPIRALAWEDGSGKVWLTVNDPAWLAQRHSLGLSSDVAIKAMVTGTGTVTKYAAGD
ATGACGCGCGAATTCATCATGCTCATGGTGCCGCCGCTGACGGACGGTTTTCCGCTCGATATTCTTGTGGCCAATGTGGTCGCCTGTTTTCTCCTCGGTACGGTGACTGCGCTTTATGCCAGGAAGATTCATTCCCGCGATGTGCACACCATAATCGGCACGGGCATGATGGGCGGGGTCTCCACCTTTTCCAGCTTTGCCTATGGCTCGGTCGTGCTGGCCTCCGCTTCGATGTCGGCTTTTCTCATCGCTGCCGCCTATGTCACGGTGAGCGTGGTTGCTGGTTATGTCGCGGTTCTGGCCGGAATGAAATTCGGTGAGAAAAGTGCAGATATTCTGCACCGATATCCCCCGATGGCGAGCATTATCGATAGCGGCCTTGTAACGGTCGAGAGCCGCCACTCCGTCGCTGAAACCATCGAACGTGTCGCGGCGAAGGCGAAGTCGATGGGGATGAATGTTTTCACCCGTGTCGATCACGGGGCCGGAGCCAAAGAGGCGGGGCTGGGCTTGCCGCCGACCGAACTCATCATTTTCGGAAACCCGCAAAACGGCACGGTTTTGATGCAGGACAAGAGAACCATCGGCCTTGATCTTCCCATTCGCGCACTTGCCTGGGAAGACGGCAGCGGCAAGGTCTGGCTCACGGTCAATGATCCGGCCTGGCTCGCACAACGCCACAGCCTGGGTTTGTCGAGTGACGTGGCTATCAAGGCGATGGTGACTGGAACCGGCACCGTCACGAAATATGCCGCCGGGGAT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 9 sequences; 10,142 total letters Query= APC114117.102 Length=1167 Score E Sequences producing significant alignments: (Bits) Value 06B_B1_APC114117.102BT7term.seq 1797 0.0 06A_A1_APC114117.102AT7term.seq 1705 0.0 01B_B1_APC114117.102BT7sh.seq 1680 0.0 01F_F1_APC114117.102DT7sh.seq 1496 0.0 01E_E1_APC114117.102CT7sh.seq 1490 0.0 06F_F1_APC114117.102DT7term.seq 1460 0.0 06E_E1_APC114117.102CT7term.seq 1460 0.0 01A_A1_APC114117.102AT7sh.seq 1402 0.0 wt 1375 0.0 S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 5 GATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAAC-AATTCCCCTCTA-GAAATAATTTTGTTTAACTTTAAGA 92 6 1090 .....N................M........A...GNNTGNN.N.....N..........NNN......................... 1003 5 1070 ...T...N.....--..T........-......-.....N.....-.....-.......-...N--.........-.............. 991 2 7 .........N-...-..................... 40 4 14 ..............-........ 35 3 3 .-.......-.G..-...-..................... 38 8 981 ...........................................-.........G..-......................... 902 8 926 ...C...........T.T.C.C.C..-.........A..-. 964 7 979 ...........................................-.........G..-......................... 900 7 924 ...C...........T.T.C.C.C..-.........A..-. 962 1 17 ................... 35 E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E Query_1 93 AGGAGATATAC-ATATGCACC-ATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCA-TCCGCAGTTCGAAAA-GGGTACCGA-G 177 6 1002 ...........-.........-......................KCN.......N.N....N-.........N.....A.........-. 917 5 990 ..A........N.......N.N.................NN-..N...N.....N.-.....A..........N....-.........A. 904 2 41 ...........-.........-........................................-...............-.........-. 125 4 52 ..-. 54 4 36 ...........-..... 51 3 55 ..-. 57 3 39 ...........-..... 54 8 885 ..-. 883 8 901 ...........-..... 886 7 883 ..-. 881 7 899 ...........-..... 884 1 36 ...........-.........-........................................-...............-.........-. 120 N L Y F Q S N A M T R E F I M L M V P P L T D G F P L D I Query_1 178 AA-CCTGTACTTCC-AATCCAATGCCATGACGCGCGAATTCATCATGCTCATGGTGCCGCCGCTGACGGACGGTTTTCCGCTCGATATTC 265 6 916 ..N...........N........................................................................... 827 5 903 ..-..........--....................................N.N..............-..................... 818 2 126 ..-...........-........................................................................... 213 4 55 ..-...........-........................................................................... 142 3 58 ..-...........-........................................................................... 145 8 882 ..-...........-........................................................................... 795 7 880 ..-...........-........................................................................... 793 1 121 ..-...........-........................................................................... 208 0 1 ................................................................ 64 L V A N V V A C F L L G T V T A L Y A R K I H S R D V H T Query_1 266 TTGTGGCCAATGTGGTCGCCTGTTTTCTCCTCGG-TACGGTGACTGCGCTTTATGCCAGGAAGATTCATTCCC-GCGATGTGCACACCAT 353 6 826 ..................................G...................N..................-................ 738 5 817 .N-................N..............-.......NM.............................C................ 730 2 214 ..................................-......................................-................ 301 4 143 ..................................-......................................-................ 230 3 146 ..................................-......................................-................ 233 8 794 ..................................-......................................-................ 707 7 792 ..................................-......................................-................ 705 1 209 ..................................-......................................-................ 296 0 65 ..................................-......................................-................ 152 I I G T G M M G G V S T F S S F A Y G S V V L A S A S M S A Query_1 354 AATCGG-CACGGGCATGATGGGCGGGGTCTCCACCTTTTCCAGCTTTGCCTATGGCTCGG-TCGTGCTGGCCTCCGCTTCGATGTCGGCT 441 6 737 ......G.....................................................G............................. 648 5 729 ......-.....................................................-.....N....................... 642 2 302 ......-.....................................................-............................. 389 4 231 ......-.....................................................-............................. 318 3 234 ......-.....................................................-............................. 321 8 706 ......-.....................................................-............................. 619 7 704 ......-.....................................................-............................. 617 1 297 ......-.....................................................-............................. 384 0 153 ......-.....................................................-............................. 240 F L I A A A Y V T V S V V A G Y V A V L A G M K F G E K S A Query_1 442 TTTCTCATCGCTGCCGCCTATGTCACGGTGAGCGTGGTTGCTGGTTATGTCGCGGTTCTGGCCGGAATGAAATTCGGTGAGAAAAGTGCA 531 6 647 ...............................N.......................................................... 558 5 641 ...........N.....N.............................N.......................................... 552 2 390 .......................................................................................... 479 4 319 .......................................................................................... 408 3 322 .......................................................................................... 411 8 618 .......................................................................................... 529 7 616 .......................................................................................... 527 1 385 .......................................................................................... 474 0 241 .......................................................................................... 330 D I L H R Y P P M A S I I D S G L V T V E S R H S V A E T I Query_1 532 GATATTCTGCACCGATATCCCCCGATGGCGAGCATTATCGATAGCGGCCTTGTAACGGTCGAGAGCCGCCACTCCGTCGCTGAAACCATC 621 6 557 .......................................................................................... 468 5 551 .......................................................................................... 462 2 480 .......................................................................................... 569 4 409 .......................................................................................... 498 3 412 .......................................................................................... 501 8 528 .......................................................................................... 439 7 526 .......................................................................................... 437 1 475 .......................................................................................... 564 0 331 .......................................................................................... 420 E R V A A K A K S M G M N V F T R V D H G A G A K E A G L G Query_1 622 GAACGTGTCGCGGCGAAGGCGAAGTCGATGGGGATGAATGTTTTCACCCGTGTCGATCACGGGGCCGGAGCCAAAGAGGCGGGGCTGGGC 711 6 467 .......................................................................................... 378 5 461 .......................................................................................... 372 2 570 .......................................................................................... 659 4 499 .......................................................................................... 588 3 502 .......................................................................................... 591 8 438 .......................................................................................... 349 7 436 .......................................................................................... 347 1 565 .......................................................................................... 654 0 421 .......................................................................................... 510 L P P T E L I I F G N P Q N G T V L M Q D K R T I G L D L Query_1 712 TTGCCGCCGACCGAACTCATCATTTTCGGAAACCCGCAAAACGGCACGG-TTTTGATGCAGGACAAGAGAACCATCGG-CCTTG-ATCTT 798 6 377 .................................................-............................-.....-..... 291 5 371 .................................................-............................-.....-..... 285 2 660 .............................................N...-............................-.....-..... 746 4 589 .................................................-............................-.....-..... 675 3 592 .................................................-............................-.....-..... 678 8 348 .................................................-............................-.....-..... 262 7 346 .................................................-............................-.....-..... 260 1 655 .................................................T..........N.................C.....N..... 744 0 511 .................................................-............................-.....-..... 597 P I R A L A W E D G S G K V W L T V N D P A W L A Q R Query_1 799 CCCA-TTCGCGCACTTGCCTGGGAAGACGGC-AGCGGCAA-GG-TCTGGCTCACGGTCAATGA-TCCGG-CCT-GGCTCGCACAACGCCA 881 6 290 ....-..........................-........-..-...................-.....-...-................ 208 5 284 ....-..........................-........-..-...................-.....-...-................ 202 2 747 ....-..........................-........G..-...................-.....-...-................ 830 4 676 ....-..........................-........-..-...................-.....-...-................ 758 3 679 ....-..........................-........-..-...................-.....-...-................ 761 8 261 ....-..........................-........-..-...................-.....-...-................ 179 7 259 ....-..........................-........-..-...................-.....-...-................ 177 1 745 ....T.......................N..A...N....-..T...................T.....AN..G..N............. 833 0 598 ....-..........................-........-..-...................-.....-...-................ 680 H S L G L S S D V A I K A M V T G T G T V T K Y A A G D * Query_1 882 CAG-CCT-GGG--TTTGTCGAGTGACGTGGCTATCAAGGCGATGGTGACTGGAACCGGCACCGTCACGAAATATGCCGCCGGGGATTAAC 967 6 207 ...-...-...--............................................................................. 122 5 201 ...-...-...--............................................................................. 116 2 831 ...-...-...--.........................................N.............-..................... 915 4 759 ...-...-...--............................................................................. 844 3 762 ...-...G...--............................................................................. 848 8 178 ...-...-...--............................................................................. 93 7 176 ...-...-...--............................................................................. 91 1 834 ...T...-...GT......N..... 857 0 681 ...-...-...--......................................................................... 762 H W K W I T D P N S S S V D K L A A A L E H H H H H H * D Query_1 968 ATTGGAAGTGGATAAC--GG-ATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATC 1054 6 121 ................--..-..................................................................... 35 5 115 ................--..-...........G......................................................... 29 2 916 ................--..A.................N......... 961 4 845 ................CT..-TAAA...AC.--...G.TG..--..AT...---------------------.A.G...G...ATG..CT 908 3 849 ................CT..-TAAA...AC.--...G.TG...A.----.TT.AA...-----.....--------------A..-..CT 911 8 92 ................ 77 7 90 ................ 75 P A A N K A R K E A E L A A A T A E Q * L A * P L G A S K R Query_1 1055 CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGG 1144 6 34 ...................... 13 5 28 ..............N....N.. 7 4 909 ..T..A...G.GC..--.TT..TT..C.----.A.................................................-...... 991 3 912 ..T..A...G.GC...---TT.AT..C..-..--................................-...........T........... 994 V L R G F L L K Query_1 1145 TCTTGAGGGGTTTTTTGCTGAAA 1167 4 992 ......-............... 1012 3 995 ....................... 1017 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11487350 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:02 AM Number of letters in database: 10,142 Number of sequences in database: 9 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC114118.102 (APC114118.1-129.F..pMCSG68)
MTPLDVMWVCLGGGVGSLGRWWIGRIVGEYHHGAFPLGTFLINISGAFVIGYLSVLFGVDWHDRYGTMLNAGVLTGILGGYTTFSSMQLDAVKLSHKGQGGLAVFYLVASVLSGLFAAWLGAMLAHLQG
ATGACTCCTTTAGATGTTATGTGGGTCTGTCTGGGCGGAGGTGTCGGATCTCTTGGGCGATGGTGGATAGGGCGTATCGTCGGTGAATATCATCACGGCGCTTTTCCGCTCGGCACGTTTCTCATCAATATTTCCGGGGCGTTCGTCATCGGATATCTGTCGGTGCTGTTTGGTGTCGACTGGCATGACCGTTACGGCACGATGCTGAATGCCGGTGTCCTCACCGGCATACTCGGCGGCTATACGACCTTCAGCAGCATGCAGCTTGATGCCGTCAAACTTTCTCATAAGGGGCAGGGCGGCCTGGCCGTGTTCTATCTGGTTGCGTCCGTCCTTTCCGGCCTGTTTGCCGCATGGCTCGGCGCAATGCTTGCCCATCTTCAGGGT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 9 sequences; 10,172 total letters Query= APC114118.102 Length=792 Score E Sequences producing significant alignments: (Bits) Value 01C_C1_APC114118.102AT7sh.seq 1317 0.0 06C_C1_APC114118.102AT7term.seq 1247 0.0 06D_D1_APC114118.102BT7term.seq 1243 0.0 01H_H1_APC114118.102DT7sh.seq 838 0.0 01G_G1_APC114118.102CT7sh.seq 838 0.0 06H_H1_APC114118.102DT7term.seq 783 0.0 06G_G1_APC114118.102CT7term.seq 783 0.0 09E_D1_APC114118.102BT7sh.seq 773 0.0 wt 699 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L Query_1 1 TCTCGATCCCGCGAAA-TTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAAC-TTT 88 1 5 .............-..............-... 34 4 713 ................A............................................N........................-... 625 4 645 ...G...N.......T.T.C.C.C...........A... 683 5 707 ................-.....................................................................-... 620 5 640 ...G...........T.T.C.C.C...........A... 678 3 12 .....-...........-... 30 2 4 ........G..--................-... 33 7 606 ....-................................................G....................-... 531 7 551 ...C...........T.T.C.C.C...........A... 589 6 605 ....-................................................G....................-... 530 6 550 ...C...........T.T.C.C.C...........A... 588 8 2 ....NN.AN....N.N..-........T..G.N. 34 * E G D I H M H H H H H H S S G V D L W S H P Q F E K Query_1 89 AAGAAGGAGA-TATACAT--ATG-CACCA-TCATCA-TCATCAT-TCTTCTGGTGT-AGATCTGTGGTCTCATCCGCAG-TTCGAAAAGG 169 1 35 ..........-.......--...-.....-......-.......-...........-......................-.......... 115 4 624 ..........-.......--...-.....-......-.......-...........-......................-.......... 544 5 619 ..........-.......--...-.....-......-.......-...........-....N.................-.......... 539 3 31 ..........-.......--... 50 2 34 ..........-.......--... 53 7 530 ..........-.......--... 511 6 529 ..........-.......--... 510 8 35 C.N.......A......NNA...G.....C......G.......N...N....AN.T..........N...N.....N.N.......... 124 G T E N L Y F Q S N A M T P L D V M W V C L G G G V G S L Query_1 170 GTACCGAGAACCTGTACTTCCAATCCAATGCCATGAC-TCC-TTTAGATGTTATGTGGGTCTGTCTGGGCGGAGGTGTCGGATCTCTTGG 257 1 116 .....................................-...-................................................ 203 4 543 .....................................-...-................................................ 456 5 538 .....................................-...-................................................ 451 3 51 ................................-...-................................................ 133 2 54 ................................-...-................................................ 136 7 510 ................................-...-................................................ 428 6 509 ................................-...-................................................ 427 8 125 ..N................N.................G...G...........N..............C........N.N.......... 214 0 1 .....-...-................................................ 56 G R W W I G R I V G E Y H H G A F P L G T F L I N I S G A F Query_1 258 GCGATGGTGGATAGGGCGTATCGTCGGTGAATATCATCACGGCGCTTTTCCGCTCGGCACGTTTCTCATCAATATTTCCGGGGCGTTCGT 347 1 204 .......................................................................................... 293 4 455 .......................................................................................... 366 5 450 .......................................................................................... 361 3 134 .......................................................................................... 223 2 137 .......................................................................................... 226 7 427 .......................................................................................... 338 6 426 .......................................................................................... 337 8 215 ............................N..........................N......N......N....N..N......N....G 304 0 57 .......................................................................................... 146 V I G Y L S V L F G V D W H D R Y G T M L N A G V L T G I L Query_1 348 CATCGGATATCTGTCGGTGCTGTTTGGTGTCGACTGGCATGACCGTTACGGCACGATGCTGAATGCCGGTGTCCTCACCGGCATACTCGG 437 1 294 .......................................................................................... 383 4 365 .......................................................................................... 276 5 360 .......................................................................................... 271 3 224 .......................................................................................... 313 2 227 .......................................................................................... 316 7 337 .......................................................................................... 248 7 767 .............T. 753 7 759 ........... 769 6 336 .......................................................................................... 247 6 766 .............T. 752 6 758 ........... 768 8 305 ..........................N..A...................N........N..........N.................... 394 0 147 .......................................................................................... 236 G G Y T T F S S M Q L D A V K L S H K G Q G G L A V F Y L V Query_1 438 CGGC-TATACGACCTTCAGCAGCATGCAGCTTGATGCCGTCAAA-CTTTCTCATAAGGGGCAGGGCGGCCTGGCCGTGTTCTATCTGGTT 525 1 384 ....-.......................................-............................................. 471 4 275 ....-.......................................-............................................. 188 5 270 ....-.......................................-............................................. 183 3 314 ....-.......................................-............................................. 401 2 317 ....-.......................................-............................................. 404 7 247 ....-.......................................-............................................. 160 7 752 .. 751 6 246 ....-.......................................-............................................. 159 6 751 .. 750 8 395 ....N.....N.........................N..N....N.N.......N.C........N........................ 484 0 237 ....-.......................................-............................................. 324 A S V L S G L F A A W L G A M L A H L Q G * H W K W I T Query_1 526 GCGTCCGTCCTTTCCGGCCTGTTTGCCGCATGGCTC-GGCGCAATGCTTGC-CCATCTTCAGGGTTAACATTGGAAGTGGATAAC--GGA 611 1 472 ....................................-..............-.................................--... 557 4 187 ....................................-..............-.................................--... 102 5 182 ....................................-..............-.................................--... 97 3 402 ....................................-..............-.................................CT..T 489 2 405 ....................................-..............-.................................CT..T 492 7 159 ....................................-..............-................................. 77 6 158 ....................................-..............-................................. 76 8 485 ....N.....N........N.NAN...N....N...NNNNNN.....C...N.......N.A...................N...--... 572 0 325 ....................................-..............-............. 387 D P N S S S V D K L A A A L E H H H H H H * D P A A N K A Query_1 612 TCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTC-GAGcac-caccaccaccaccacTGAGATCCGGCTGCTAA-CAAA--GCC 696 1 558 ........................................-......-................................-....--... 642 4 101 ........................................-......-................................-....--... 17 5 96 ........................................-......-................................T....--NG. 11 3 490 AAA...AC.--...G.TG..--..AT...-----------------------.A.G...G...ATG..CT..T..A...G-.GC.--.-- 547 2 493 AAA...AC.--...G.TG..--..AT...-----------------------.A.G...G...ATG..CT..T..A...G-.GC.--.-- 550 8 573 NN....N..A..N........N........NNT...NT..TN.....TN....N..NN.N......N..A.....NNNN.-.TN.TG... 661 R K E A E L A A A T A E Q * L A * P L G A S K R V L R G Query_1 697 CGAAAGGAAGCTG-AGTTGGCTGCTGCCACCGCTGA-GCAATAA-CTAGCATAACCCCTTGGGG-CCTCTAAACGGGTCTTGAGGGGTTT 782 1 643 .............-......................-.......-...................-......................... 728 4 16 . 16 5 10 .C.... 5 3 548 .TT..TT..C.-----.A..................-.......-...................-......................... 629 2 551 .TT..TT..C.-----.A..................-.......-...................-......................... 632 8 662 .....A....N..A.................NN...NN......NN...........NN...NNC........ 734 F L L K Query_1 783 TTTGCTGAAA 792 1 729 .......... 738 3 630 .......... 639 2 633 .......... 642 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 7764725 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:02 AM Number of letters in database: 10,172 Number of sequences in database: 9 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115038.119 (APC115038.26-783.P(785).D292A.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVTGDITTGQAKSSDVAQKIEKGLVGGLFNLKGVDRILEVQKLAVEKSRLGIPLLFGMDVIHGYETIFPIPLGLSCTWDMAAIEKSARIAAIEASADGISWTFSPMVDISRDPRWGRVSEGSGEDPFLGGAIAQAMVYGYQGANLQDQLHRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEFMYPYEAAVEAGVGSVMASFNEIDGIPATGNKWLLSDLLRGQWGFEGFVVTDFTGISEMIEHGVGDLQTVSALALNAGVDMDMVSEGFVGTLMKSIKEGKVRMGTLNTACRRILEAKYKLGLFDNPYKYCDVNRPKRDIFTKEHRDAARKIAGESFVLLKNAPATAQPLAAHSSSPVTASPVLPLKKQGTVAVIGPLGNTRSNMPGTWSVAARLNDYPSLYEGLKEMMAGKVNITYAKGSNLIGDAAYEERATMFGRSLNRDNRTDQELLDEALKIAAGADVIVAALGESSEMSGESSSRTELGLPDVQHTLLEALLKTGKPVVLTLFTGRPLTLNWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLAAGKWFEKFRSNYLDVDNEPLYPFGYGLSYTTFQYSDIALSTPTLGKDGSVTAVVTVTNTGKHDGAEVVQLYIRDLVGSITRPVRELKGFNKIFLRAGESKTVSFTITRDLLRFYDYDLNYVAEPGDFDIMIGGNSQAVKTAKLTL
AAGTCACCGCAAGACATGGATCGCTTCATCGACGCACTGATGAAGAAGATGACCGTGGAAGAGAAAATCGGACAATTGAACCTACCCGTCACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCACAAAAGATTGAAAAAGGATTGGTGGGCGGACTCTTCAACCTAAAAGGTGTAGACCGTATTCTTGAAGTGCAAAAGCTGGCAGTAGAGAAATCACGCCTCGGTATTCCCCTGCTGTTCGGCATGGATGTGATACATGGCTACGAAACCATCTTCCCCATTCCATTGGGATTGTCCTGCACCTGGGATATGGCGGCTATCGAGAAATCCGCCCGTATTGCAGCCATCGAAGCAAGTGCCGATGGCATTTCCTGGACATTCAGTCCGATGGTAGACATCAGTCGCGACCCACGTTGGGGACGTGTCAGCGAGGGCTCGGGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTATACGGATACCAGGGTGCCAATCTGCAAGACCAATTGCACCGCAACGATGAAATCATGGCTTGCGTAAAACACTTTGCATTGTATGGAGCCGGAGAAGCCGGACGCGACTATAATACAGTAGATATGAGCCGCAACCGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAGGCCGGAGTGGGTAGTGTGATGGCGTCATTCAATGAAATAGACGGTATTCCCGCCACCGGAAACAAATGGCTGCTGAGCGATTTGCTGCGTGGCCAGTGGGGCTTCGAAGGGTTTGTGGTAACGGACTTCACAGGCATTTCAGAGATGATAGAGCATGGTGTCGGCGACTTGCAAACCGTCAGTGCACTCGCTCTTAATGCAGGGGTGGACATGGATATGGTAAGTGAGGGCTTCGTCGGTACACTGATGAAATCAATTAAAGAAGGAAAAGTAAGAATGGGCACGTTGAATACAGCCTGCCGCCGGATATTGGAAGCGAAATACAAGCTGGGACTGTTTGACAATCCTTATAAATACTGCGACGTGAACCGTCCGAAGCGGGATATCTTCACAAAAGAGCATCGTGACGCCGCCCGCAAGATTGCCGGCGAAAGTTTTGTTCTTCTGAAGAATGCCCCCGCCACCGCACAGCCACTCGCAGCTCATAGCTCGTCACCCGTAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAAGGTACAGTTGCCGTCATCGGCCCTCTCGGAAATACCCGCAGCAACATGCCGGGCACCTGGAGCGTAGCCGCACGCCTCAACGATTATCCTTCTTTATACGAAGGCTTGAAAGAAATGATGGCAGGCAAGGTGAACATCACCTATGCCAAAGGTAGTAACCTCATCGGCGATGCAGCTTACGAAGAACGTGCCACCATGTTCGGCCGTTCATTGAACCGCGATAATCGCACGGACCAGGAGTTACTGGACGAAGCACTGAAAATTGCAGCCGGCGCCGATGTTATCGTAGCTGCCCTGGGAGAATCTTCTGAAATGAGCGGTGAAAGTTCAAGCCGCACCGAACTCGGCTTGCCCGATGTACAACATACCCTGTTGGAAGCCTTACTGAAAACGGGTAAACCCGTAGTACTAACCCTCTTTACCGGTCGGCCGTTGACGCTGAACTGGGAACAGGAGCATGTACCTGCCATCTTGAATGTATGGTTCGGAGGCAGTGAAGCGGCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTCAATCCGAGTGGAAAACTAACCATGACTTTCCCGAAGAATGTAGGCCAGATACCTTTGTTCTACAATCATAAGAATACCGGTCGGCCACTGGCGGCAGGCAAATGGTTCGAAAAGTTCCGTTCAAACTATCTGGATGTGGATAACGAACCGCTGTATCCCTTCGGTTATGGATTGTCGTATACCACTTTCCAGTACAGTGACATTGCATTGAGCACACCGACATTGGGAAAAGATGGTTCCGTTACAGCCGTAGTCACCGTCACCAATACTGGTAAACATGACGGTGCGGAAGTAGTTCAACTCTATATCCGCGACCTCGTAGGAAGTATCACCCGCCCTGTACGCGAGTTGAAAGGTTTCAATAAAATCTTCCTTCGCGCCGGAGAAAGCAAAACGGTATCATTCACTATCACGCGTGATCTGCTTCGCTTCTATGATTACGACCTGAACTACGTAGCCGAACCGGGTGACTTTGACATCATGATCGGTGGAAACAGCCAGGCTGTGAAGACGGCGAAGTTGACACTT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,742 total letters Query= APC115038.119 Length=2679 Score E Sequences producing significant alignments: (Bits) Value wt 4093 0.0 K S P Q D M D R F I D A L M K K M T V E E K I G Q L N L P V Query_1 202 AAGTCACCGCAAGACATGGATCGCTTCATCGACGCACTGATGAAGAAGATGACCGTGGAAGAGAAAATCGGACAATTGAACCTACCCGTC 291 0 1 .......................................................................................... 90 T G D I T T G Q A K S S D V A Q K I E K G L V G G L F N L K Query_1 292 ACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCACAAAAGATTGAAAAAGGATTGGTGGGCGGACTCTTCAACCTAAAA 381 0 91 .......................................................................................... 180 G V D R I L E V Q K L A V E K S R L G I P L L F G M D V I H Query_1 382 GGTGTAGACCGTATTCTTGAAGTGCAAAAGCTGGCAGTAGAGAAATCACGCCTCGGTATTCCCCTGCTGTTCGGCATGGATGTGATACAT 471 0 181 .......................................................................................... 270 G Y E T I F P I P L G L S C T W D M A A I E K S A R I A A I Query_1 472 GGCTACGAAACCATCTTCCCCATTCCATTGGGATTGTCCTGCACCTGGGATATGGCGGCTATCGAGAAATCCGCCCGTATTGCAGCCATC 561 0 271 .......................................................................................... 360 E A S A D G I S W T F S P M V D I S R D P R W G R V S E G S Query_1 562 GAAGCAAGTGCCGATGGCATTTCCTGGACATTCAGTCCGATGGTAGACATCAGTCGCGACCCACGTTGGGGACGTGTCAGCGAGGGCTCG 651 0 361 .......................................................................................... 450 G E D P F L G G A I A Q A M V Y G Y Q G A N L Q D Q L H R N Query_1 652 GGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTATACGGATACCAGGGTGCCAATCTGCAAGACCAATTGCACCGCAAC 741 0 451 .......................................................................................... 540 D E I M A C V K H F A L Y G A G E A G R D Y N T V D M S R N Query_1 742 GATGAAATCATGGCTTGCGTAAAACACTTTGCATTGTATGGAGCCGGAGAAGCCGGACGCGACTATAATACAGTAGATATGAGCCGCAAC 831 0 541 .......................................................................................... 630 R M F N E F M Y P Y E A A V E A G V G S V M A S F N E I D G Query_1 832 CGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAGGCCGGAGTGGGTAGTGTGATGGCGTCATTCAATGAAATAGACGGT 921 0 631 .......................................................................................... 720 I P A T G N K W L L S D L L R G Q W G F E G F V V T A F T G Query_1 922 ATTCCCGCCACCGGAAACAAATGGCTGCTGAGCGATTTGCTGCGTGGCCAGTGGGGCTTCGAAGGGTTTGTGGTAACGGCTTTCACAGGC 1011 0 721 ...............................................................................AC......... 810 I S E M I E H G V G D L Q T V S A L A L N A G V D M D M V S Query_1 1012 ATTTCAGAGATGATAGAGCATGGTGTCGGCGACTTGCAAACCGTCAGTGCACTCGCTCTTAATGCAGGGGTGGACATGGATATGGTAAGT 1101 0 811 .......................................................................................... 900 E G F V G T L M K S I K E G K V R M G T L N T A C R R I L E Query_1 1102 GAGGGCTTCGTCGGTACACTGATGAAATCAATTAAAGAAGGAAAAGTAAGAATGGGCACGTTGAATACAGCCTGCCGCCGGATATTGGAA 1191 0 901 .......................................................................................... 990 A K Y K L G L F D N P Y K Y C D V N R P K R D I F T K E H R Query_1 1192 GCGAAATACAAGCTGGGACTGTTTGACAATCCTTATAAATACTGCGACGTGAACCGTCCGAAGCGGGATATCTTCACAAAAGAGCATCGT 1281 0 991 .......................................................................................... 1080 D A A R K I A G E S F V L L K N A P A T A Q P L A A H S S S Query_1 1282 GACGCCGCCCGCAAGATTGCCGGCGAAAGTTTTGTTCTTCTGAAGAATGCCCCCGCCACCGCACAGCCACTCGCAGCTCATAGCTCGTCA 1371 0 1081 .......................................................................................... 1170 P V T A S P V L P L K K Q G T V A V I G P L G N T R S N M P Query_1 1372 CCCGTAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAAGGTACAGTTGCCGTCATCGGCCCTCTCGGAAATACCCGCAGCAACATGCCG 1461 0 1171 .......................................................................................... 1260 G T W S V A A R L N D Y P S L Y E G L K E M M A G K V N I T Query_1 1462 GGCACCTGGAGCGTAGCCGCACGCCTCAACGATTATCCTTCTTTATACGAAGGCTTGAAAGAAATGATGGCAGGCAAGGTGAACATCACC 1551 0 1261 .......................................................................................... 1350 Y A K G S N L I G D A A Y E E R A T M F G R S L N R D N R T Query_1 1552 TATGCCAAAGGTAGTAACCTCATCGGCGATGCAGCTTACGAAGAACGTGCCACCATGTTCGGCCGTTCATTGAACCGCGATAATCGCACG 1641 0 1351 .......................................................................................... 1440 D Q E L L D E A L K I A A G A D V I V A A L G E S S E M S G Query_1 1642 GACCAGGAGTTACTGGACGAAGCACTGAAAATTGCAGCCGGCGCCGATGTTATCGTAGCTGCCCTGGGAGAATCTTCTGAAATGAGCGGT 1731 0 1441 .......................................................................................... 1530 E S S S R T E L G L P D V Q H T L L E A L L K T G K P V V L Query_1 1732 GAAAGTTCAAGCCGCACCGAACTCGGCTTGCCCGATGTACAACATACCCTGTTGGAAGCCTTACTGAAAACGGGTAAACCCGTAGTACTA 1821 0 1531 .......................................................................................... 1620 T L F T G R P L T L N W E Q E H V P A I L N V W F G G S E A Query_1 1822 ACCCTCTTTACCGGTCGGCCGTTGACGCTGAACTGGGAACAGGAGCATGTACCTGCCATCTTGAATGTATGGTTCGGAGGCAGTGAAGCG 1911 0 1621 .......................................................................................... 1710 0 1821 ............ 1832 A Y A I G D V L F G D V N P S G K L T M T F P K N V G Q I P Query_1 1912 GCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTCAATCCGAGTGGAAAACTAACCATGACTTTCCCGAAGAATGTAGGCCAGATACCT 2001 0 1711 .......................................................................................... 1800 L F Y N H K N T G R P L A A G K W F E K F R S N Y L D V D N Query_1 2002 TTGTTCTACAATCATAAGAATACCGGTCGGCCACTGGCGGCAGGCAAATGGTTCGAAAAGTTCCGTTCAAACTATCTGGATGTGGATAAC 2091 0 1801 .......................................................................................... 1890 0 1629 ............ 1640 E P L Y P F G Y G L S Y T T F Q Y S D I A L S T P T L G K D Query_1 2092 GAACCGCTGTATCCCTTCGGTTATGGATTGTCGTATACCACTTTCCAGTACAGTGACATTGCATTGAGCACACCGACATTGGGAAAAGAT 2181 0 1891 .......................................................................................... 1980 G S V T A V V T V T N T G K H D G A E V V Q L Y I R D L V G Query_1 2182 GGTTCCGTTACAGCCGTAGTCACCGTCACCAATACTGGTAAACATGACGGTGCGGAAGTAGTTCAACTCTATATCCGCGACCTCGTAGGA 2271 0 1981 .......................................................................................... 2070 S I T R P V R E L K G F N K I F L R A G E S K T V S F T I T Query_1 2272 AGTATCACCCGCCCTGTACGCGAGTTGAAAGGTTTCAATAAAATCTTCCTTCGCGCCGGAGAAAGCAAAACGGTATCATTCACTATCACG 2361 0 2071 .......................................................................................... 2160 R D L L R F Y D Y D L N Y V A E P G D F D I M I G G N S Q A Query_1 2362 CGTGATCTGCTTCGCTTCTATGATTACGACCTGAACTACGTAGCCGAACCGGGTGACTTTGACATCATGATCGGTGGAAACAGCCAGGCT 2451 0 2161 .......................................................................................... 2250 V K T A K L T L Query_1 2452 GTGAAGACGGCGAAGTTGACACTT 2475 0 2251 ........................ 2274 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 15135768 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:02 AM Number of letters in database: 5,742 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115038.131 (APC115038.26-783.P(785).E536Q.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVTGDITTGQAKSSDVAQKIEKGLVGGLFNLKGVDRILEVQKLAVEKSRLGIPLLFGMDVIHGYETIFPIPLGLSCTWDMAAIEKSARIAAIEASADGISWTFSPMVDISRDPRWGRVSEGSGEDPFLGGAIAQAMVYGYQGANLQDQLHRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEFMYPYEAAVEAGVGSVMASFNEIDGIPATGNKWLLSDLLRGQWGFEGFVVTDFTGISEMIEHGVGDLQTVSALALNAGVDMDMVSEGFVGTLMKSIKEGKVRMGTLNTACRRILEAKYKLGLFDNPYKYCDVNRPKRDIFTKEHRDAARKIAGESFVLLKNAPATAQPLAAHSSSPVTASPVLPLKKQGTVAVIGPLGNTRSNMPGTWSVAARLNDYPSLYEGLKEMMAGKVNITYAKGSNLIGDAAYEERATMFGRSLNRDNRTDQELLDEALKIAAGADVIVAALGESSEMSGESSSRTELGLPDVQHTLLEALLKTGKPVVLTLFTGRPLTLNWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLAAGKWFEKFRSNYLDVDNEPLYPFGYGLSYTTFQYSDIALSTPTLGKDGSVTAVVTVTNTGKHDGAEVVQLYIRDLVGSITRPVRELKGFNKIFLRAGESKTVSFTITRDLLRFYDYDLNYVAEPGDFDIMIGGNSQAVKTAKLTL
AAGTCACCGCAAGACATGGATCGCTTCATCGACGCACTGATGAAGAAGATGACCGTGGAAGAGAAAATCGGACAATTGAACCTACCCGTCACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCACAAAAGATTGAAAAAGGATTGGTGGGCGGACTCTTCAACCTAAAAGGTGTAGACCGTATTCTTGAAGTGCAAAAGCTGGCAGTAGAGAAATCACGCCTCGGTATTCCCCTGCTGTTCGGCATGGATGTGATACATGGCTACGAAACCATCTTCCCCATTCCATTGGGATTGTCCTGCACCTGGGATATGGCGGCTATCGAGAAATCCGCCCGTATTGCAGCCATCGAAGCAAGTGCCGATGGCATTTCCTGGACATTCAGTCCGATGGTAGACATCAGTCGCGACCCACGTTGGGGACGTGTCAGCGAGGGCTCGGGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTATACGGATACCAGGGTGCCAATCTGCAAGACCAATTGCACCGCAACGATGAAATCATGGCTTGCGTAAAACACTTTGCATTGTATGGAGCCGGAGAAGCCGGACGCGACTATAATACAGTAGATATGAGCCGCAACCGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAGGCCGGAGTGGGTAGTGTGATGGCGTCATTCAATGAAATAGACGGTATTCCCGCCACCGGAAACAAATGGCTGCTGAGCGATTTGCTGCGTGGCCAGTGGGGCTTCGAAGGGTTTGTGGTAACGGACTTCACAGGCATTTCAGAGATGATAGAGCATGGTGTCGGCGACTTGCAAACCGTCAGTGCACTCGCTCTTAATGCAGGGGTGGACATGGATATGGTAAGTGAGGGCTTCGTCGGTACACTGATGAAATCAATTAAAGAAGGAAAAGTAAGAATGGGCACGTTGAATACAGCCTGCCGCCGGATATTGGAAGCGAAATACAAGCTGGGACTGTTTGACAATCCTTATAAATACTGCGACGTGAACCGTCCGAAGCGGGATATCTTCACAAAAGAGCATCGTGACGCCGCCCGCAAGATTGCCGGCGAAAGTTTTGTTCTTCTGAAGAATGCCCCCGCCACCGCACAGCCACTCGCAGCTCATAGCTCGTCACCCGTAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAAGGTACAGTTGCCGTCATCGGCCCTCTCGGAAATACCCGCAGCAACATGCCGGGCACCTGGAGCGTAGCCGCACGCCTCAACGATTATCCTTCTTTATACGAAGGCTTGAAAGAAATGATGGCAGGCAAGGTGAACATCACCTATGCCAAAGGTAGTAACCTCATCGGCGATGCAGCTTACGAAGAACGTGCCACCATGTTCGGCCGTTCATTGAACCGCGATAATCGCACGGACCAGGAGTTACTGGACGAAGCACTGAAAATTGCAGCCGGCGCCGATGTTATCGTAGCTGCCCTGGGAGAATCTTCTGAAATGAGCGGTGAAAGTTCAAGCCGCACCGAACTCGGCTTGCCCGATGTACAACATACCCTGTTGGAAGCCTTACTGAAAACGGGTAAACCCGTAGTACTAACCCTCTTTACCGGTCGGCCGTTGACGCTGAACTGGGAACAGGAGCATGTACCTGCCATCTTGAATGTATGGTTCGGAGGCAGTGAAGCGGCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTCAATCCGAGTGGAAAACTAACCATGACTTTCCCGAAGAATGTAGGCCAGATACCTTTGTTCTACAATCATAAGAATACCGGTCGGCCACTGGCGGCAGGCAAATGGTTCGAAAAGTTCCGTTCAAACTATCTGGATGTGGATAACGAACCGCTGTATCCCTTCGGTTATGGATTGTCGTATACCACTTTCCAGTACAGTGACATTGCATTGAGCACACCGACATTGGGAAAAGATGGTTCCGTTACAGCCGTAGTCACCGTCACCAATACTGGTAAACATGACGGTGCGGAAGTAGTTCAACTCTATATCCGCGACCTCGTAGGAAGTATCACCCGCCCTGTACGCGAGTTGAAAGGTTTCAATAAAATCTTCCTTCGCGCCGGAGAAAGCAAAACGGTATCATTCACTATCACGCGTGATCTGCTTCGCTTCTATGATTACGACCTGAACTACGTAGCCGAACCGGGTGACTTTGACATCATGATCGGTGGAAACAGCCAGGCTGTGAAGACGGCGAAGTTGACACTT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,036 total letters Query= APC115038.131 Length=2679 Score E Sequences producing significant alignments: (Bits) Value wt 4093 0.0 02B_B2_APC115038.131_B06.seq 21.1 5.8 K S P Q D M D R F I D A L M K K M T V E E K I G Q L N L P V Query_1 202 AAGTCACCGCAAGACATGGATCGCTTCATCGACGCACTGATGAAGAAGATGACCGTGGAAGAGAAAATCGGACAATTGAACCTACCCGTC 291 0 1 .......................................................................................... 90 T G D I T T G Q A K S S D V A Q K I E K G L V G G L F N L K Query_1 292 ACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCACAAAAGATTGAAAAAGGATTGGTGGGCGGACTCTTCAACCTAAAA 381 0 91 .......................................................................................... 180 G V D R I L E V Q K L A V E K S R L G I P L L F G M D V I H Query_1 382 GGTGTAGACCGTATTCTTGAAGTGCAAAAGCTGGCAGTAGAGAAATCACGCCTCGGTATTCCCCTGCTGTTCGGCATGGATGTGATACAT 471 0 181 .......................................................................................... 270 G Y E T I F P I P L G L S C T W D M A A I E K S A R I A A I Query_1 472 GGCTACGAAACCATCTTCCCCATTCCATTGGGATTGTCCTGCACCTGGGATATGGCGGCTATCGAGAAATCCGCCCGTATTGCAGCCATC 561 0 271 .......................................................................................... 360 E A S A D G I S W T F S P M V D I S R D P R W G R V S E G S Query_1 562 GAAGCAAGTGCCGATGGCATTTCCTGGACATTCAGTCCGATGGTAGACATCAGTCGCGACCCACGTTGGGGACGTGTCAGCGAGGGCTCG 651 0 361 .......................................................................................... 450 G E D P F L G G A I A Q A M V Y G Y Q G A N L Q D Q L H R N Query_1 652 GGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTATACGGATACCAGGGTGCCAATCTGCAAGACCAATTGCACCGCAAC 741 0 451 .......................................................................................... 540 D E I M A C V K H F A L Y G A G E A G R D Y N T V D M S R N Query_1 742 GATGAAATCATGGCTTGCGTAAAACACTTTGCATTGTATGGAGCCGGAGAAGCCGGACGCGACTATAATACAGTAGATATGAGCCGCAAC 831 0 541 .......................................................................................... 630 R M F N E F M Y P Y E A A V E A G V G S V M A S F N E I D G Query_1 832 CGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAGGCCGGAGTGGGTAGTGTGATGGCGTCATTCAATGAAATAGACGGT 921 0 631 .......................................................................................... 720 I P A T G N K W L L S D L L R G Q W G F E G F V V T D F T G Query_1 922 ATTCCCGCCACCGGAAACAAATGGCTGCTGAGCGATTTGCTGCGTGGCCAGTGGGGCTTCGAAGGGTTTGTGGTAACGGACTTCACAGGC 1011 0 721 .......................................................................................... 810 1 611 ........... 621 I S E M I E H G V G D L Q T V S A L A L N A G V D M D M V S Query_1 1012 ATTTCAGAGATGATAGAGCATGGTGTCGGCGACTTGCAAACCGTCAGTGCACTCGCTCTTAATGCAGGGGTGGACATGGATATGGTAAGT 1101 0 811 .......................................................................................... 900 E G F V G T L M K S I K E G K V R M G T L N T A C R R I L E Query_1 1102 GAGGGCTTCGTCGGTACACTGATGAAATCAATTAAAGAAGGAAAAGTAAGAATGGGCACGTTGAATACAGCCTGCCGCCGGATATTGGAA 1191 0 901 .......................................................................................... 990 A K Y K L G L F D N P Y K Y C D V N R P K R D I F T K E H R Query_1 1192 GCGAAATACAAGCTGGGACTGTTTGACAATCCTTATAAATACTGCGACGTGAACCGTCCGAAGCGGGATATCTTCACAAAAGAGCATCGT 1281 0 991 .......................................................................................... 1080 D A A R K I A G E S F V L L K N A P A T A Q P L A A H S S S Query_1 1282 GACGCCGCCCGCAAGATTGCCGGCGAAAGTTTTGTTCTTCTGAAGAATGCCCCCGCCACCGCACAGCCACTCGCAGCTCATAGCTCGTCA 1371 0 1081 .......................................................................................... 1170 P V T A S P V L P L K K Q G T V A V I G P L G N T R S N M P Query_1 1372 CCCGTAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAAGGTACAGTTGCCGTCATCGGCCCTCTCGGAAATACCCGCAGCAACATGCCG 1461 0 1171 .......................................................................................... 1260 G T W S V A A R L N D Y P S L Y E G L K E M M A G K V N I T Query_1 1462 GGCACCTGGAGCGTAGCCGCACGCCTCAACGATTATCCTTCTTTATACGAAGGCTTGAAAGAAATGATGGCAGGCAAGGTGAACATCACC 1551 0 1261 .......................................................................................... 1350 Y A K G S N L I G D A A Y E E R A T M F G R S L N R D N R T Query_1 1552 TATGCCAAAGGTAGTAACCTCATCGGCGATGCAGCTTACGAAGAACGTGCCACCATGTTCGGCCGTTCATTGAACCGCGATAATCGCACG 1641 0 1351 .......................................................................................... 1440 D Q E L L D E A L K I A A G A D V I V A A L G E S S E M S G Query_1 1642 GACCAGGAGTTACTGGACGAAGCACTGAAAATTGCAGCCGGCGCCGATGTTATCGTAGCTGCCCTGGGAGAATCTTCTGAAATGAGCGGT 1731 0 1441 .......................................................................................... 1530 Q S S S R T E L G L P D V Q H T L L E A L L K T G K P V V L Query_1 1732 CAGAGTTCAAGCCGCACCGAACTCGGCTTGCCCGATGTACAACATACCCTGTTGGAAGCCTTACTGAAAACGGGTAAACCCGTAGTACTA 1821 0 1531 G.A....................................................................................... 1620 T L F T G R P L T L N W E Q E H V P A I L N V W F G G S E A Query_1 1822 ACCCTCTTTACCGGTCGGCCGTTGACGCTGAACTGGGAACAGGAGCATGTACCTGCCATCTTGAATGTATGGTTCGGAGGCAGTGAAGCG 1911 0 1621 .......................................................................................... 1710 0 1821 ............ 1832 A Y A I G D V L F G D V N P S G K L T M T F P K N V G Q I P Query_1 1912 GCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTCAATCCGAGTGGAAAACTAACCATGACTTTCCCGAAGAATGTAGGCCAGATACCT 2001 0 1711 .......................................................................................... 1800 L F Y N H K N T G R P L A A G K W F E K F R S N Y L D V D N Query_1 2002 TTGTTCTACAATCATAAGAATACCGGTCGGCCACTGGCGGCAGGCAAATGGTTCGAAAAGTTCCGTTCAAACTATCTGGATGTGGATAAC 2091 0 1801 .......................................................................................... 1890 0 1629 ............ 1640 E P L Y P F G Y G L S Y T T F Q Y S D I A L S T P T L G K D Query_1 2092 GAACCGCTGTATCCCTTCGGTTATGGATTGTCGTATACCACTTTCCAGTACAGTGACATTGCATTGAGCACACCGACATTGGGAAAAGAT 2181 0 1891 .......................................................................................... 1980 G S V T A V V T V T N T G K H D G A E V V Q L Y I R D L V G Query_1 2182 GGTTCCGTTACAGCCGTAGTCACCGTCACCAATACTGGTAAACATGACGGTGCGGAAGTAGTTCAACTCTATATCCGCGACCTCGTAGGA 2271 0 1981 .......................................................................................... 2070 S I T R P V R E L K G F N K I F L R A G E S K T V S F T I T Query_1 2272 AGTATCACCCGCCCTGTACGCGAGTTGAAAGGTTTCAATAAAATCTTCCTTCGCGCCGGAGAAAGCAAAACGGTATCATTCACTATCACG 2361 0 2071 .......................................................................................... 2160 R D L L R F Y D Y D L N Y V A E P G D F D I M I G G N S Q A Query_1 2362 CGTGATCTGCTTCGCTTCTATGATTACGACCTGAACTACGTAGCCGAACCGGGTGACTTTGACATCATGATCGGTGGAAACAGCCAGGCT 2451 0 2161 .......................................................................................... 2250 V K T A K L T L Query_1 2452 GTGAAGACGGCGAAGTTGACACTT 2475 0 2251 ........................ 2274 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 13270070 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:02 AM Number of letters in database: 5,036 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115043.112 (APC115043.26-768.P(769).D292A.pMCSG68)
KSGDASMNKFIDKLMDRMTLEEKIGQLNLPSSGDITTGQARSNNIADKIRAGAVGGLFNIKGVEKIQEVQRIAVEESRLKIPLLFGMDVIHGYETVFPIPLGMAATWDMKAIEQSARIAAIEASADGICWTFSPMVDISRDPRWGRVSEGSGEDPFLGGEIAKAMVYGYQGKGDSAYREKTNIMACVKHYALYGAAEAGLDYNTTDMSRIRMFNEYMYPYQAAVDAGAGSVMSSFNEVDGIPATANKWLITDVLRKQWGFGGFVVTDYTGIMEMVNHGIGDMREVSARALSAGVDMDMVSEGYLSTLQQSLKEGKITEKEIDQACRRILEAKYKLGLFDNPYKYCDTERAKTDIYTDEHRSIARRISAESFVLLKNDKQTLPIKKKGKIAVVGPLANTSSNMPGTWSVAVNMEAPATLVEGLKEVAGDKVEIVYAKGSHLMSDAAYEERATLFGRTLYRDKEKRSDIQMLNEALNVAHGADVVVAALGESSEMSGESSSRTDLNIPDVQKTLLEELVKTGKPVVLVLFTGRPLTLTWEDKNVSAILNVWFGGTEAAYAIGDVLFGNVNPGGKLPVTFPQNVGQIPLFYNHKNTGRPLAEGGWFEKFRANYLDVTNEPLYPFGYGLSYAQFDYSDVRLSTDQIDRNGMLTASVTVTNNSECDGDEIVQLYIRDLVGSVTRPVKELKGFEKVTIRAGESKDISFKITPEMLKFYNSDIQFVNEVGEFEVMIGTNSRDVKKATFSL
AAGAGTGGGGATGCGTCGATGAACAAATTTATTGATAAACTGATGGACAGGATGACCTTGGAAGAGAAGATTGGTCAGCTTAATCTTCCCAGCTCGGGAGATATAACCACCGGACAGGCACGCAGCAACAATATTGCAGACAAAATCAGAGCAGGTGCAGTGGGTGGCTTATTCAATATAAAAGGAGTTGAGAAGATACAGGAAGTACAACGTATTGCTGTAGAGGAGAGTCGCCTGAAAATTCCTTTACTCTTTGGCATGGATGTTATTCATGGGTATGAAACTGTTTTCCCTATTCCTTTGGGTATGGCTGCCACATGGGATATGAAGGCTATAGAACAATCTGCTCGTATAGCGGCGATAGAAGCCAGTGCCGATGGCATCTGCTGGACATTTAGTCCGATGGTTGATATCAGCCGTGATCCACGTTGGGGACGTGTATCCGAAGGTAGCGGAGAAGATCCTTTTTTAGGTGGTGAAATTGCTAAGGCGATGGTATATGGCTATCAGGGTAAAGGTGATAGCGCATATCGTGAAAAGACTAATATTATGGCTTGTGTGAAGCACTATGCCTTGTATGGGGCAGCAGAAGCCGGTTTGGACTATAATACAACTGACATGAGCCGTATTCGTATGTTTAATGAATATATGTATCCTTATCAGGCGGCTGTGGATGCGGGTGCCGGCAGTGTCATGTCTTCTTTCAACGAGGTCGATGGAATTCCTGCAACAGCCAACAAATGGTTGATAACTGATGTCCTGCGTAAACAGTGGGGATTCGGTGGTTTTGTCGTTACGGACTATACCGGTATCATGGAAATGGTAAATCATGGTATTGGAGATATGCGAGAAGTCTCTGCCCGTGCTTTGAGTGCAGGAGTGGATATGGATATGGTGAGCGAAGGTTATCTTTCTACACTTCAACAATCATTGAAGGAGGGTAAGATAACAGAGAAAGAGATAGATCAAGCTTGCCGTCGTATTTTGGAGGCAAAATATAAGCTGGGATTATTTGATAATCCTTATAAGTATTGTGATACTGAACGTGCCAAAACGGATATCTACACTGATGAACATCGGAGTATTGCACGCCGGATCTCTGCTGAAAGCTTTGTTCTTTTAAAGAATGATAAACAGACACTGCCTATAAAGAAAAAAGGTAAGATTGCTGTAGTTGGGCCGTTGGCGAATACGAGTTCTAATATGCCCGGAACGTGGAGTGTAGCGGTCAATATGGAAGCTCCAGCTACGCTTGTGGAGGGTTTGAAAGAAGTGGCAGGTGATAAAGTTGAAATTGTGTATGCTAAGGGTAGCCATCTGATGAGTGATGCGGCTTATGAGGAACGTGCAACACTCTTTGGACGTACATTATACCGGGATAAGGAAAAACGTTCCGATATCCAGATGCTGAATGAAGCATTAAATGTTGCTCATGGTGCCGATGTTGTTGTTGCGGCATTAGGTGAATCTTCTGAAATGAGTGGTGAATCGAGTAGTCGAACAGATTTGAATATTCCTGATGTTCAAAAAACATTATTGGAAGAATTAGTGAAAACAGGTAAACCTGTCGTTCTGGTATTATTCACTGGGCGTCCGTTGACCCTGACATGGGAAGACAAAAATGTATCTGCTATTCTGAATGTTTGGTTTGGAGGTACCGAAGCCGCTTATGCTATAGGAGATGTCCTATTCGGAAATGTAAATCCTGGAGGTAAGCTGCCTGTAACATTTCCTCAGAATGTAGGGCAGATTCCTTTATTCTATAACCATAAAAATACTGGACGTCCGCTGGCTGAGGGCGGTTGGTTTGAGAAGTTCCGGGCAAATTATCTGGATGTAACGAATGAACCTCTTTATCCATTTGGCTATGGACTAAGTTATGCACAATTTGATTATAGCGATGTGAGATTAAGTACGGATCAAATAGACCGGAATGGCATGTTAACCGCAAGTGTGACTGTAACCAATAACAGTGAGTGTGATGGAGATGAAATTGTTCAGTTGTATATTCGCGATTTGGTCGGTAGTGTTACTCGTCCGGTGAAAGAATTGAAAGGATTTGAAAAAGTAACAATTAGAGCAGGGGAGTCAAAAGATATTTCTTTTAAGATCACTCCGGAAATGCTTAAGTTCTACAATTCGGATATCCAGTTTGTGAATGAAGTTGGTGAATTCGAAGTAATGATCGGAACGAACAGCAGGGATGTGAAAAAAGCAACGTTTAGCTTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,410 total letters Query= APC115043.112 Length=2634 Score E Sequences producing significant alignments: (Bits) Value wt 4011 0.0 02C_C2_APC115043.112_C06.seq 1483 0.0 07C_C2_APC115043.112_C08.seq 1328 0.0 E K G T E N L Y F Q S N A K S G D A S M N K F I Query_1 162 CGAAAAGGG---TACCGAG-AACCTGT-ACTT--CCAATCC--AATGCCAAGAGT---GGGGATG-CGT-CGATGAACAAA--TTTATT- 234 0 1 ......---.......-...-...........--......- 33 2 992 .........GGN.C...G.N.......T....TC.......CA.NN........GNGG.......A...T....N...N..AT......N 903 D K L M D R M T L E E K I G Q L N L P S S G D I T Query_1 235 GATAAA-CTGAT-GGAC-AGGATGA-CCTTGG---AAGAGAAGATTGG-TCAG--CTTAAT-CTTCCC-AGCT-CGG-GAG-ATATAACC 309 0 34 ......-.....-....-.......-......---.............-....--......-......-....-...-...-........ 108 2 902 .....NT.NN..T....C.......N....N.GNA.G.N.........N....GCN.....A......C.N..N...T...N....N... 813 T G Q A R S N N I A D K I R A G A V G G L F N I K G Query_1 310 ACC--GGACAGGCA-CGC-AGCAA-CAATA-TTGCAGACAAA-ATC-AG-AGCAGGTGCAGTGGGTGG-CTTATTCAATATAAAA-GGAG 388 0 109 ...--.........-...-.....-.....-...........-...-..-..................-................-.... 187 2 812 CT.CG.........N...C.A...N.....N.........N.N...C..C...............N..G................A.... 723 V E K I Q E V Q R I A V E E S R L K I P L L F G M D V I H Query_1 389 TTGAGAAGATACA-GGAAGTA-CAACGTATTGCTGTAGAGGAGAGTCGCCTGAAAA-TTCCTTTACTCTTTGGCATGGA-TGTTATTCAT 474 0 188 .............-.......-..................................-......................-.......... 273 2 722 ...N.........N.......N..................................A.....................GC.......... 633 G Y E T V F P I P L G M A A T W D M K A I E Q S A R I A A Query_1 475 GGGTATGAAA-CTGTTTTCCCTATTCCTTTGGGTATGGCTGCCACATGGGATATGAAGGCTATAGAACAATCTGCTCGTATAGCGGCGAT 563 0 274 ..........-............................................................................... 362 2 632 ..........A............................................................................... 543 I E A S A D G I C W T F S P M V D I S R D P R W G R V S E G Query_1 564 AGAAGCCAGTGCCGATGGCATCTGCTGGACATTTAGTCCGATGGTTGATATCAGCCGTGATCCACGTTGGGGACGTGTATCCGAAGGTAG 653 0 363 .......................................................................................... 452 2 542 .......................................................................................... 453 S G E D P F L G G E I A K A M V Y G Y Q G K G D S A Y R E K Query_1 654 CGGAGAAGATCCTTTTTTAGGTGGTGAAATTGCTAAGGCGATGGTATATGGCTATCAGGGTAAAGGTGATAGCGCATATCGTGAAAAGAC 743 0 453 .......................................................................................... 542 2 452 .......................................................................................... 363 T N I M A C V K H Y A L Y G A A E A G L D Y N T T D M S R I Query_1 744 TAATATTATGGCTTGTGTGAAGCACTATGCCTTGTATGGGGCAGCAGAAGCCGGTTTGGACTATAATACAACTGACATGAGCCGTATTCG 833 0 543 .......................................................................................... 632 2 362 .......................................................................................... 273 R M F N E Y M Y P Y Q A A V D A G A G S V M S S F N E V D G Query_1 834 TATGTTTAATGAATATATGTATCCTTATCAGGCGGCTGTGGATGCGGGTGCCGGCAGTGTCATGTCTTCTTTCAACGAGGTCGATGGAAT 923 0 633 .......................................................................................... 722 2 272 .......................................................................................... 183 I P A T A N K W L I T D V L R K Q W G F G G F V V T A Y T G Query_1 924 TCCTGCAACAGCCAACAAATGGTTGATAACTGATGTCCTGCGTAAACAGTGGGGATTCGGTGGTTTTGTCGTTACGGCT-TATACCGGTA 1012 0 723 .............................................................................AC-.......... 811 1 9 .......-.....................................................................AC-.......... 96 2 182 .............................................................................A.C.......... 93 I M E M V N H G I G D M R E V S A R A L S A G V D M D M V S Query_1 1013 TCATGGAAATGGTAAATCATGGTATTGGAGATATGCGAGAAGTCTCTGCCCGTGCTTTGAGTGCAGGA-GTGGATATGGATATGGTGAGC 1101 0 812 ....................................................................-..................... 900 1 97 ....................................................................-..................... 185 2 92 ....................................................................T................-N... 4 E G Y L S T L Q Q S L K E G K I T E K E I D Q A C R R I L E Query_1 1102 GAAGGTTATCTTTCTACACTTCAACAATCATTGAAGGAGGGTAAGATAACAGAGAAAGAGATAGATCAAGCTTGCCGTCGTATTTTGGAG 1191 0 901 .......................................................................................... 990 1 186 .......................................................................................... 275 2 3 ... 1 A K Y K L G L F D N P Y K Y C D T E R A K T D I Y T D E H R Query_1 1192 GCAAAATATAAGCTGGGATTATTTGATAATCCTTATAAGTATTGTGATACTGAACGTGCCAAAACGGATATCTACACTGATGAACATCGG 1281 0 991 .......................................................................................... 1080 1 276 .......................................................................................... 365 S I A R R I S A E S F V L L K N D K Q T L P I K K K G K I A Query_1 1282 AGTATTGCACGCCGGATCTCTGCTGAAAGCTTTGTTCTTTTAAAGAATGATAAACAGACACTGCCTATAAAGAAAAAAGGTAAGATTGCT 1371 0 1081 .......................................................................................... 1170 1 366 .......................................................................................... 455 V V G P L A N T S S N M P G T W S V A V N M E A P A T L V E Query_1 1372 GTAGTTGGGCCGTTGGCGAATACGAGTTCTAATATGCCCGGAACGTGGAGTGTAGCGGTCAATATGGAAGCTCCAGCTACGCTTGTGGAG 1461 0 1171 .......................................................................................... 1260 1 456 .......................................................................................... 545 G L K E V A G D K V E I V Y A K G S H L M S D A A Y E E R A Query_1 1462 GGTTTGAAAGAAGTGGCAGGTGATAAAGTTGAAATTGTGTATGCTAAGGGTAGCCATCTGATGAGTGATGCGGCTTATGAGGAACGTGCA 1551 0 1261 .......................................................................................... 1350 1 546 .......................................................................................... 635 T L F G R T L Y R D K E K R S D I Q M L N E A L N V A H G A Query_1 1552 ACACTCTTTGGACGTACATTATACCGGGATAAGGAAAAACGTTCCGATATCCAGATGCTGAATGAAGCATTAAATGTTGCTCATGGTGCC 1641 0 1351 .......................................................................................... 1440 1 636 .......................................................................................... 725 D V V V A A L G E S S E M S G E S S S R T D L N I P D V Q K Query_1 1642 GATGTTGTTGTTGCGGCATTAGGTGAATCTTCTGAAATGAGTGGTGAATCGAGTAGTCGAACAGATTTGAATATTCCTGATGTTCAAAAA 1731 0 1441 .......................................................................................... 1530 1 726 ..........................................................................N............... 815 T L L E E L V K T G K P V V L V L F T G R P L T L T W E D K Query_1 1732 ACATTATTGGAAGAATTAGTGAAAACAGGTAAACCTGTCGTTCTGGTATTATTCACTGGGCGTCCGTTGACCCTGACATGGGAAGACAAA 1821 0 1531 .......................................................................................... 1620 1 816 ..N...............N........... 845 N V S A I L N V W F G G T E A A Y A I G D V L F G N V N P G Query_1 1822 AATGTATCTGCTATTCTGAATGTTTGGTTTGGAGGTACCGAAGCCGCTTATGCTATAGGAGATGTCCTATTCGGAAATGTAAATCCTGGA 1911 0 1621 .......................................................................................... 1710 G K L P V T F P Q N V G Q I P L F Y N H K N T G R P L A E G Query_1 1912 GGTAAGCTGCCTGTAACATTTCCTCAGAATGTAGGGCAGATTCCTTTATTCTATAACCATAAAAATACTGGACGTCCGCTGGCTGAGGGC 2001 0 1711 .......................................................................................... 1800 G W F E K F R A N Y L D V T N E P L Y P F G Y G L S Y A Q F Query_1 2002 GGTTGGTTTGAGAAGTTCCGGGCAAATTATCTGGATGTAACGAATGAACCTCTTTATCCATTTGGCTATGGACTAAGTTATGCACAATTT 2091 0 1801 .......................................................................................... 1890 D Y S D V R L S T D Q I D R N G M L T A S V T V T N N S E C Query_1 2092 GATTATAGCGATGTGAGATTAAGTACGGATCAAATAGACCGGAATGGCATGTTAACCGCAAGTGTGACTGTAACCAATAACAGTGAGTGT 2181 0 1891 .......................................................................................... 1980 D G D E I V Q L Y I R D L V G S V T R P V K E L K G F E K V Query_1 2182 GATGGAGATGAAATTGTTCAGTTGTATATTCGCGATTTGGTCGGTAGTGTTACTCGTCCGGTGAAAGAATTGAAAGGATTTGAAAAAGTA 2271 0 1981 .......................................................................................... 2070 T I R A G E S K D I S F K I T P E M L K F Y N S D I Q F V N Query_1 2272 ACAATTAGAGCAGGGGAGTCAAAAGATATTTCTTTTAAGATCACTCCGGAAATGCTTAAGTTCTACAATTCGGATATCCAGTTTGTGAAT 2361 0 2071 .......................................................................................... 2160 E V G E F E V M I G T N S R D V K K A T F S L Query_1 2362 GAAGTTGGTGAATTCGAAGTAATGATCGGAACGAACAGCAGGGATGTGAAAAAAGCAACGTTTAGCTTG 2430 0 2161 ..................................................................... 2229 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11407503 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:02 AM Number of letters in database: 4,410 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115044.110 (APC115044.33-744.P(748).D104A.pMCSG68)
VESLLSKMTLEEKIGQMNQISSYGNIEDMSALIKKGEIGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQVAKDGARIAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKNVLRDEWGFDGFVVTDWASASEMISHGFAAGSKEVAMKSVNAGVDMEMVSYTFVKELPELVKEGKVKESTIDEAVRNILRIKYRLGLFDTPYVDEQQTSVMYAPSHLEAAKQAAVESAILLKNDKEVLPLQPSVKTVAVVGPMANAPYEQLGTWIFDGEKARTQTPLNAIKEMVGDKVQVIYEPGLAYSREKNPASVAKAAAAAARADVILAFVGEESILSGEAHCLADLDLQGDQGALITALAKTGKPVVTIVMAGRPLTIGKEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKAPVTFPRMVGQIPVYYAHNNTGRPATRNEVLLNDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNIKLASDVLKKDDVLTVTFDLENTGKYEGTEVAQLYIQDKIGSVTRPVKELKRFTRVTLKPGEKKSVSFELPVSELAFWNIDMAKVVEPGDFGLWVATDSQSGEEVFF
GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGTGCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAATCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCGTTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATTGATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGCAGCTATGGTAGAAGGTTTTCAGGGAGATTCGCTGAATAGTCCTACTTCAATTGCAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAGCCGAAGGAGGACGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAGCGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAATGTACTCCGTGACGAGTGGGGATTCGATGGTTTTGTTGTGACGGACTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGTTCAAAAGAAGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTGAAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCTTATGTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTGAAGAATGATAAGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTAGGTACTTGGATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATTTATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTGAATGATATTGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTTGTCGTACACCACATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATTCGATCTGGAAAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGAAAAAAAGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCTTC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,926 total letters Query= APC115044.110 Length=2541 Score E Sequences producing significant alignments: (Bits) Value wt 3847 0.0 07D_D2_APC115044.110_D08.seq 884 0.0 02D_D2_APC115044.110T7sh.seq 774 0.0 G D I H M H H H H H H S S G V D L W S H P Q F E K G Query_1 94 GGAGATATACATA-TGCACCA-TCATCA-TCATCAT-TCT-TC-TGG-TG-TAGATC-TG-TGG-TCTCATCCGCAGTTCGAAAAGGGTA 172 1 42 ............NC..NN...G......N.N.N...A..NN..A...A..C......N..A...C....N.......N............ 131 T E N L Y F Q S N A V E S L L S K M T L E E K I G Q M N Q Query_1 173 CCGAG-AACC-TG-TACTTCCAATCCAATGCCGTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGA 259 0 1 .......................................................... 58 1 132 .....N....G..A..................................................N......................... 221 I S S Y G N I E D M S A L I K K G E I G S I L N E V D P V Query_1 260 TTTCCTCTTACGGTAATATCGAGGATATGAGTGCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGG-ATCCGGT-GCG 347 0 59 ..............................................................................-.......-... 146 2 703 ..........N.......N.NN 682 1 222 .............G.........................N.................................N....-.......-... 309 R I N A L Q R V A M E E S R L G I P L L I A R A V I H G Query_1 348 TATT--AATGCGCTACA--GCGCGTGGC--AATGGAAGAATCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGCTGTCATTCACGGGTT 431 0 147 ....--...........--.........--............................................A............... 230 2 681 ..N.TA........N.CNG.N.N...N.CA.....N.................N........N.......N.GN.......N.N.N.N.. 592 1 310 ....--.....N.....--........N--...............................N..........N................. 393 F K T I F P I P L G Q A A S F N P Q V A K D G A R I A A I Query_1 432 TAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTT-CGTTCAATCCGCAGGTAGCGA-AAGACGGTGCACGGATAGCAGC-TATTGA 518 0 231 ......................................-.....................-......................-...... 317 2 591 ....N...N......N........N..N.......N..-.....................TN......N..N.....N....NN...N.. 503 1 394 ....N..N..........N...................N.N...N.N.........N...-.N.....A...N........T.-...... 481 E A S S V G I R W T F A P M I D I A R D P R W G R I A E G C Query_1 519 AGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATTGATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGG 608 0 318 .......................................................................................... 407 2 502 ...N.....N.....................N.A....N.........NNN.............N.N.C..................... 413 1 482 ......N......C.C.N...............A.............................T...N...NA...........N..... 571 G E D T Y L T S V M G A A M V E G F Q G D S L N S P T S I Query_1 609 TGAAGAT-AC-GTACCTTACTTCCGTAATGGGAGCAGCTATGGTAGAAGGTTTTCA-GGGAGATTCGCTG-AATAGTCCTACTTCAATTG 694 0 408 .......-..-.............................................-.............-................... 493 2 412 N......G..-............N.N..N............N..............-.......N.....-N.................. 326 1 572 ....C..-..AT............NN.....N....N........T.N........N....N.N...N..A.....C.......NN... 659 A A C P K H F V G Y G A A E G G R D Y N S T F I P E R R L R Query_1 695 CAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAGCCGAAGGAGGACGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCA 784 0 494 .......................................................................................... 583 2 325 ...............................N.......................................................... 236 N V Y L P P F E A A T K A G A A T F M T S F N D N D G I P S Query_1 785 ATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAGCGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTA 874 0 584 .......................................................................................... 673 2 235 ........................................................G................................. 146 T G N A F I L K N V L R D E W G F D G F V V T D W A S A S Query_1 875 CCGGGAATGCTTTTATTTTGAAGAATGTACTCCGTGACGAGTGGGGA-TTCGATGGTTTTGTTGTGA-CGGACTGGGCTTCTGCCAGCGA 962 0 674 ...............................................-...................-...................... 761 2 145 ...............................................G.............N.....C...T....N...N.......N. 56 E M I S H G F A A G S K E V A M K S V N A G V D M E M V S Y Query_1 963 AATGATAAGCCATG-GTTTTGCCGCCGGTTCAAAAGAAGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACA 1051 0 762 ..............-........................................................................... 850 2 55 ..N...G.N...C.AA...NN..C..T.... 25 T F V K E L P E L V K E G K V K E S T I D E A V R N I L R I Query_1 1052 CTTTTGTGAAGGAACTGCCGGAATTAGTGAAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAA 1141 0 851 .......................................................................................... 940 K Y R L G L F D T P Y V D E Q Q T S V M Y A P S H L E A A K Query_1 1142 AGTATCGTTTAGGATTGTTTGATACACCTTATGTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGC 1231 0 941 .......................................................................................... 1030 Q A A V E S A I L L K N D K E V L P L Q P S V K T V A V V G Query_1 1232 AAGCCGCTGTTGAATCGGCTATTCTGTTGAAGAATGATAAGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGAC 1321 0 1031 .......................................................................................... 1120 P M A N A P Y E Q L G T W I F D G E K A R T Q T P L N A I K Query_1 1322 CTATGGCTAATGCACCTTATGAACAGTTAGGTACTTGGATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAG 1411 0 1121 .......................................................................................... 1210 E M V G D K V Q V I Y E P G L A Y S R E K N P A S V A K A A Query_1 1412 AAATGGTTGGCGATAAAGTACAGGTGATTTATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTG 1501 0 1211 .......................................................................................... 1300 A A A A R A D V I L A F V G E E S I L S G E A H C L A D L D Query_1 1502 CCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATT 1591 0 1301 .......................................................................................... 1390 L Q G D Q G A L I T A L A K T G K P V V T I V M A G R P L T Query_1 1592 TGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTA 1681 0 1391 .......................................................................................... 1480 I G K E V E E S T A V L Y S F H P G T M G G P A L A D L L W Query_1 1682 TCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGG 1771 0 1481 .......................................................................................... 1570 G K A V P S G K A P V T F P R M V G Q I P V Y Y A H N N T G Query_1 1772 GGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGAC 1861 0 1571 .......................................................................................... 1660 R P A T R N E V L L N D I A V E A G Q T S L G C T S F Y M D Query_1 1862 GTCCGGCTACACGGAATGAAGTGTTGCTGAATGATATTGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATG 1951 0 1661 .......................................................................................... 1750 A G F D P L F P F G Y G L S Y T T F K Y S N I K L A S D V L Query_1 1952 CGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTTGTCGTACACCACATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGA 2041 0 1751 .......................................................................................... 1840 K K D D V L T V T F D L E N T G K Y E G T E V A Q L Y I Q D Query_1 2042 AAAAAGATGATGTGCTGACAGTGACATTCGATCTGGAAAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATA 2131 0 1841 .......................................................................................... 1930 K I G S V T R P V K E L K R F T R V T L K P G E K K S V S F Query_1 2132 AGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGaaaaaaaGCGTTTCGTTTG 2221 0 1931 .......................................................................................... 2020 E L P V S E L A F W N I D M A K V V E P G D F G L W V A T D Query_1 2222 AACTCCCTGTTAGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATA 2311 0 2021 .......................................................................................... 2110 S Q S G E E V F F Query_1 2312 GTCAGTCCGGAGAAGAAGTTTTCTTC 2337 0 2111 .......................... 2136 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12304500 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:02 AM Number of letters in database: 4,926 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115044.117 (APC115044.33-744.P(748).W418F.pMCSG68)
VESLLSKMTLEEKIGQMNQISSYGNIEDMSALIKKGEIGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQVAKDGARIAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKNVLRDEWGFDGFVVTDWASASEMISHGFAAGSKEVAMKSVNAGVDMEMVSYTFVKELPELVKEGKVKESTIDEAVRNILRIKYRLGLFDTPYVDEQQTSVMYAPSHLEAAKQAAVESAILLKNDKEVLPLQPSVKTVAVVGPMANAPYEQLGTWIFDGEKARTQTPLNAIKEMVGDKVQVIYEPGLAYSREKNPASVAKAAAAAARADVILAFVGEESILSGEAHCLADLDLQGDQGALITALAKTGKPVVTIVMAGRPLTIGKEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKAPVTFPRMVGQIPVYYAHNNTGRPATRNEVLLNDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNIKLASDVLKKDDVLTVTFDLENTGKYEGTEVAQLYIQDKIGSVTRPVKELKRFTRVTLKPGEKKSVSFELPVSELAFWNIDMAKVVEPGDFGLWVATDSQSGEEVFF
GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGTGCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAATCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCGTTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATTGATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGCAGCTATGGTAGAAGGTTTTCAGGGAGATTCGCTGAATAGTCCTACTTCAATTGCAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAGCCGAAGGAGGACGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAGCGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAATGTACTCCGTGACGAGTGGGGATTCGATGGTTTTGTTGTGACGGACTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGTTCAAAAGAAGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTGAAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCTTATGTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTGAAGAATGATAAGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTAGGTACTTGGATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATTTATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTGAATGATATTGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTTGTCGTACACCACATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATTCGATCTGGAAAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGAAAAAAAGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCTTC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,427 total letters Query= APC115044.117 Length=2541 Score E Sequences producing significant alignments: (Bits) Value wt 3844 0.0 07E_E2_APC115044.117_E08.seq 1366 0.0 02E_E2_APC115044.117_E06.seq 1361 0.0 V E S L L S K M T L E E K I G Q M N Q I S S Y G N I E D M S Query_1 202 GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGT 291 0 1 .......................................................................................... 90 A L I K K G E I G S I L N E V D P V R I N A L Q R V A M E E Query_1 292 GCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAA 381 0 91 .......................................................................................... 180 S R L G I P L L I A R D V I H G F K T I F P I P L G Q A A S Query_1 382 TCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCG 471 0 181 .......................................................................................... 270 F N P Q V A K D G A R I A A I E A S S V G I R W T F A P M I Query_1 472 TTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATT 561 0 271 .......................................................................................... 360 2 912 .... 909 D I A R D P R W G R I A E G C G E D T Y L T S V M Query_1 562 G-ATATTGCCC-GCGAT-CCT-CGCTGGGG---ACGTATTGCCG-AAGGGTG-TGGTGAAGATACGT-ACCTTA--CTTCCGTAA-TGGG 638 0 361 .-.........-.....-...-........---...........-.......-..............-......--.........-.... 437 2 908 .G..N......C.....N...N....N...GGA....T..N...N.....N.G...N........N.N...N.TNC.........CN..N 819 G A A M V E G F Q G D S L N S P T S I A A C P K H F V G Query_1 639 AGCAGCTATGGTAGAAGGTTTTCAGGG--AGA-TTCG-CTGAAT-AGTCC-TAC-TTCAATTGCAGCTTGCCCTAAACATTTTGTAGG-T 720 0 438 ...........................--...-....-......-.....-...-.................................-. 519 2 818 ...........................GA...N....G......T.....C...N.................................G. 729 Y G A A E G G R D Y N S T F I P E R R L R N V Y L P P F Query_1 721 TACGGTGCAGCCGAAGG--AGGACGTGATTATAATTCC-ACGTTCATTCCCGAA-CGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGA 806 0 520 .................--...................-...............-................................... 605 2 728 ...............A.GA...................C...............A................................N.. 639 E A A T K A G A A T F M T S F N D N D G I P S T G N A F I L Query_1 807 AGCTGCCACCAAAGCGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAA 896 0 606 .......................................................................................... 695 2 638 .......................................................................................... 549 K N V L R D E W G F D G F V V T D W A S A S E M I S H G F A Query_1 897 GAATGTACTCCGTGACGAGTGGGGATTCGATGGTTTTGTTGTGACGGACTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGC 986 0 696 .......................................................................................... 785 2 548 ..............................................................................C.....N..... 459 A G S K E V A M K S V N A G V D M E M V S Y T F V K E L P E Query_1 987 CGGTTCAAAAGAAGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATT 1076 0 786 .......................................................................................... 875 2 458 .......................................................................................... 369 L V K E G K V K E S T I D E A V R N I L R I K Y R L G L F D Query_1 1077 AGTGAAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATAC 1166 0 876 .......................................................................................... 965 2 368 .......................................................................................... 279 T P Y V D E Q Q T S V M Y A P S H L E A A K Q A A V E S A I Query_1 1167 ACCTTATGTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCT 1256 0 966 .......................................................................................... 1055 2 278 .......................................................................................... 189 1 2 ....-.N... 10 L L K N D K E V L P L Q P S V K T V A V V G P M A N A P Y E Query_1 1257 GTTGAAGAATGATAAGGAAGTGTTGCCG-TTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAA- 1344 0 1056 ............................-............................................................- 1143 2 188 ............................-............................................................T 100 1 11 .....C--......-.............C............................................................- 96 Q L G T F I F D G E K A R T Q T P L N A I K E M V G D K V Q Query_1 1345 CAGTTAGGTACTTTTATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAG 1434 0 1144 .............GG........................................................................... 1233 2 99 .........................................................................-.......-....N... 12 1 97 .......................................................................................... 186 V I Y E P G L A Y S R E K N P A S V A K A A A A A A R A D V Query_1 1435 GTGATTTATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTC 1524 0 1234 .......................................................................................... 1323 0 1318 ............ 1307 2 11 .. 10 1 187 .......................................................................................... 276 1 271 ............ 260 I L A F V G E E S I L S G E A H C L A D L D L Q G D Q G A L Query_1 1525 ATTCTTGCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTG 1614 0 1324 .......................................................................................... 1413 1 277 .......................................................................................... 366 I T A L A K T G K P V V T I V M A G R P L T I G K E V E E S Query_1 1615 ATTACAGCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCG 1704 0 1414 .......................................................................................... 1503 1 367 .......................................................................................... 456 T A V L Y S F H P G T M G G P A L A D L L W G K A V P S G K Query_1 1705 ACTGCTGTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAG 1794 0 1504 .......................................................................................... 1593 1 457 .......................................................................................... 546 A P V T F P R M V G Q I P V Y Y A H N N T G R P A T R N E Query_1 1795 GCGCCGGTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAG-T 1883 0 1594 ........................................................................................-. 1682 0 1666 ............ 1655 1 547 .....N.........................N........................................................T. 636 1 619 ............ 608 V L L N D I A V E A G Q T S L G C T S F Y M D A G F D P L F Query_1 1884 GTTGCTGAATGATATTGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGG-TTTTGATCCCTTGTTT- 1971 0 1683 ........................................................................-................- 1770 1 637 ........................................................................T................C 726 P F G Y G L S Y T T F K Y S N I K L A S D V L K K D D V Query_1 1972 CCGTTTGGTTAT-GGCTTGTCGTACACCACATTTAAGTAT-AGCAACATCAAACTG-GCGT-CTGATGTACTGAAAAAAGATGATGTGCT 2057 0 1771 ............-...........................-...............-....-............................ 1856 1 727 ............N......N.T........N.....C...A...............C..N.C.N........N................C 816 L T V T F D L E N T G K Y E G T E V A Q L Y I Q D K I G S Query_1 2058 GACAGTGACA-TTCGAT-CTGGAAAATACC--GGGAAATATG-AAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCC 2142 0 1857 ..........-......-............--..........-............................................... 1941 0 1926 ............ 1915 1 817 N..CCGN...N......C............CN..A......NA...... 865 V T R P V K E L K R F T R V T L K P G E K K S V S F E L P V Query_1 2143 GTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGaaaaaaaGCGTTTCGTTTGAACTCCCTGTT 2232 0 1942 .......................................................................................... 2031 S E L A F W N I D M A K V V E P G D F G L W V A T D S Q S G Query_1 2233 AGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGA 2322 0 2032 .......................................................................................... 2121 E E V F F * H W K W I T D P N S S S V D K L A A A L E H H H Query_1 2323 GAAGAAGTTTTCTTCTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccac 2412 0 2122 ............... 2136 H H H * D P A A N K A R K E Query_1 2413 caccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAG 2455 2 718 ........... 708 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11045024 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:02 AM Number of letters in database: 4,427 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115045.110 (APC115045.26-772.P(773).K213A.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVSGEIVTGQAQNSDVAKKIEQGLVGGLLNLKGVEKIRDVQKLAIEKSRLGIPLIFGMDVVHGYETIFPIPLGLSCSWDMEAIRKSARVAAIEASADGISWTFSPMVDISRDPRWGRVSEGNGEDPFLGGAIAKAMVSGYQGIDLNNQLKRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEYMYPYQAAVDAGVGSVMASFNEIDGIPATANKWLMTDVLRKQWGFDGFVVTDFTGISEMIAHGIGDLQTVSARALNAGVDMDMVSEGFTGTIKKSIDEGKISMETLDKACRRILEAKYKLGLFDNPYKYCDLKRPKRDIFTKEHRDAARKIAGESFVLLKNDKSGSSANPTLPLKKEGTVAVIGPLANTRSNMPGTWSVAARLNDYPSVYEGLKEMMKGKVNITYAKGSNLISDAAYEERATMFGRSLNRDNRTDKEMLDEALKVAANADVIIAALGESSEMSGESSSRTNLALPDVQRTLLEALLKTGKPVVLTLFTGRPLTLTWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLLEGKWFEKFRSNYLDVDNDPLYPFGYGLSYTNFQYSDITLSAPTMGQDGSVTAMVTVTNTGKYDGAEVVQLYIRDLVGSITRPVKELKGFDKIFLKAGESKTVSFKITPELLRFYDYELNYVAEPGDFDIMIGGNSQSVKTTHLSL
AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTTTCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAAGGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCATGGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATCGAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAACGGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAACGATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAACCGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGCATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGTATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGTGAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAAGCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGCGACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAAAAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGACTATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCAGCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,860 total letters Query= APC115045.110 Length=2646 Score E Sequences producing significant alignments: (Bits) Value wt 4028 0.0 02F_F2_APC115045.110_F06.seq 1427 0.0 07F_F2_APC115045.110_F08.seq 1409 0.0 K S P Q D M D R F I D A L M K K M T V E E K I G Q L N L P V Query_1 202 AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTT 291 0 1 .......................................................................................... 90 S G E I V T G Q A Q N S D V A K K I E Q G L V G G L L N L K Query_1 292 TCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAA 381 0 91 .......................................................................................... 180 G V E K I R D V Q K L A I E K S R L G I P L I F G M D V V H Query_1 382 GGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCAT 471 0 181 .......................................................................................... 270 G Y E T I F P I P L G L S C S W D M E A I R K S A R V A A I Query_1 472 GGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATC 561 0 271 .......................................................................................... 360 E A S A D G I S W T F S P M V D I S R D P R W G R V S E Query_1 562 GAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGT-GAT-CCGCGCT--GGGGACGCG--TCAGCGAG 645 0 361 .........................................................-...-.......--.........--........ 444 2 907 ......T...T......NTG.......GNGT.......A 869 G N G E D P F L G G A I A K A M V S G Y Q G I D L Query_1 646 GG--TAAC-GGC-GAAGACCCA--TTCTTGGGT--GGAGCCATCG-CTAAA-GCAATGG-TATCGGG-TTAT-CAGGGTATCGA-CCTCA 721 0 445 ..--....-...-.........--.........--..........-.....-.......-.......-....-...........-..... 520 1 1 ...--...C.--...-...N--.......-.......-....-...........-....G 49 2 868 ..GT....C...N........CNT........GTG..........G.....A.......G.......G....T........N..A...NG 779 N N Q L K R N D E I M A C V A H F A L Y G A G E A G R D Y N Query_1 722 ACAA-CCAACTGAAGCGCAACGATGAAATTATGGCATGTGTAGCTCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAAT 810 0 521 ....-.....................................AAG............................................. 609 1 50 ....-..................................................................................... 138 2 778 ....A......N.............................................................................. 689 T V D M S R N R M F N E Y M Y P Y Q A A V D A G V G S V M A Query_1 811 ACCGTAGATATGAGTCGTAACCGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCG 900 0 610 .......................................................................................... 699 1 139 .......................................................................................... 228 2 688 ....C..................................................................................... 599 S F N E I D G I P A T A N K W L M T D V L R K Q W G F D G F Query_1 901 TCTTTCAACGAAATAGACGGCATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTT 990 0 700 .......................................................................................... 789 1 229 .......................................................................................... 318 2 598 .......................................................................................... 509 V V T D F T G I S E M I A H G I G D L Q T V S A R A L N A G Query_1 991 GTGGTGACAGACTTTACCGGTATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGC 1080 0 790 .......................................................................................... 879 1 319 .......................................................................................... 408 2 508 .......................................................................................... 419 V D M D M V S E G F T G T I K K S I D E G K I S M E T L D K Query_1 1081 GTGGATATGGACATGGTAAGTGAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAA 1170 0 880 .......................................................................................... 969 1 409 .......................................................................................... 498 2 418 .......................................................................................... 329 A C R R I L E A K Y K L G L F D N P Y K Y C D L K R P K R D Query_1 1171 GCCTGTCGCCGCATCCTTGAAGCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGAC 1260 0 970 .......................................................................................... 1059 1 499 .......................................................................................... 588 2 328 .......................................................................................... 239 I F T K E H R D A A R K I A G E S F V L L K N D K S G S S A Query_1 1261 ATCTTCACCAAGGAACATCGCGACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCA 1350 0 1060 .......................................................................................... 1149 1 589 .......................................................................................... 678 2 238 .......................................................................................... 149 N P T L P L K K E G T V A V I G P L A N T R S N M P G T W Query_1 1351 AACCCAACACTTCCTTTGAAAAAAGAAGGTACGGTGGCTGTCATCGG-CCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCT-GGA 1438 0 1150 ...............................................-......................................-... 1237 1 679 ........................N......................C.............N.....N..................G... 768 2 148 ...............................................-......................................-... 61 S V A A R L N D Y P S V Y E G L K E M M K G K V N I T Query_1 1439 GTGTAGCCGCACGCCTCAACGACTATCCTT-CTGTGTACGAA-GGATTG-AAAGAGA-TGA-TGAAAGGCAA--GGTAAACA-TCAC-TT 1519 0 1238 ..............................-...........-......-.......-...-..........--........-....-.. 1318 1 769 N.................N...........N...........G......N..N..N.T...T..........NG........T..N.T.. 858 2 60 ..............................N........N..-..-N..-...T...-.. 5 Y A K G S N L I S D A A Y E E R A T M F G R S L N Query_1 1520 ATGCC--AAAGGTAGT-AACCTCAT-CAGT-GATGCAG--CCTACG-AAGAACG-TGCCACAA--TGTTC-GGCCG--TTCATT--AAAT 1593 0 1319 .....--.........-........-....-.......--......-.......-........--.....-.....--......--.... 1392 1 859 .....CA..G....NNA........C....G....GN.NC......A..N..NNT....N...AT.....G....NGT......TA.... 948 R D N R T D K E M L D E A L K V A A N A D V I I A A L G E S Query_1 1594 CGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCA 1683 0 1393 .......................................................................................... 1482 1 949 .. 950 S E M S G E S S S R T N L A L P D V Q R T L L E A L L K T G Query_1 1684 TCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGA 1773 0 1483 .......................................................................................... 1572 K P V V L T L F T G R P L T L T W E Q E H V P A I L N V W F Query_1 1774 AAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTC 1863 0 1573 .......................................................................................... 1662 G G S E A A Y A I G D V L F G D V N P S G K L T M T F P K N Query_1 1864 GGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAAC 1953 0 1663 .......................................................................................... 1752 V G Q I P L F Y N H K N T G R P L L E G K W F E K F R S N Y Query_1 1954 GTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTAC 2043 0 1753 .......................................................................................... 1842 L D V D N D P L Y P F G Y G L S Y T N F Q Y S D I T L S A P Query_1 2044 CTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCG 2133 0 1843 .......................................................................................... 1932 T M G Q D G S V T A M V T V T N T G K Y D G A E V V Q L Y I Query_1 2134 ACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATC 2223 0 1933 .......................................................................................... 2022 R D L V G S I T R P V K E L K G F D K I F L K A G E S K T V Query_1 2224 CGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTA 2313 0 2023 .......................................................................................... 2112 S F K I T P E L L R F Y D Y E L N Y V A E P G D F D I M I G Query_1 2314 TCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGG 2403 0 2113 .......................................................................................... 2202 G N S Q S V K T T H L S L Query_1 2404 GGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG 2442 0 2203 ....................................... 2241 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12642861 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:02 AM Number of letters in database: 4,860 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200122.105 (CMR200122.20-750.P(750).D106A.pMCSG68)
TQVANKGSDAATEKKVESLLSRMTLEEKIGQMNQITSYGNIEDMSSLIKKGEVGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQIAKDGARVAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKDVLRGEWGFDGLVVTDWASASEMISHGFAADSKEVAMKSVNAGVDMEMVSYTFVKELPALIKEGKVKESTIDEAVRNILRVKYRLGLFDVPYVDEKQPSVMYDPSHLKVAKQAAVESAILLKNDKEVLPLQESLKTIAVVGPMANAPYEQLGTWIFDGEKAHTQTPLNAIKEIVGDKVQVIYEPGLAYSREKNPAGVAKAAAVAARADVILAFVGEEAILSGEAHCLADLNLQGDQSALITALAKTGKPVVTIVMAGRPLTIGQEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKTPVTFPKMVGQIPVYYAHNNTGRPATRNEVLLDDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNVKLSSASLKKDDVLTVTFDLENTGKYKGTEVAQLYIQDKVGSVTRPVKELKRFTRVTLKPGEKKNVSFELPVSELAFWNIDMVKVVEPGDFGLWVATDSQSGEEVFFKVVD
ACACAAGTGGCAAATAAAGGTAGCGATGCGGCAACCGAGAAAAAAGTAGAGTCTCTTTTATCCAGAATGACCCTTGAAGAGAAAATCGGTCAGATGAACCAGATTACCTCTTACGGGAATATTGAGGATATGAGTAGTTTAATTAAGAAAGGTGAAGTCGGGTCTATCCTGAATGAGGTGGATCCGGTACGTATTAATGCGTTGCAACGCGTAGCGATGGAGGAGTCCCGGTTGGGAATCCCTTTGTTGATAGCTCGCGATGTTATTCACGGGTTTAAAACCATTTTTCCCATCCCATTGGGACAAGCGGCTTCGTTCAATCCGCAGATTGCGAAAGACGGTGCACGGGTAGCGGCTATTGAGGCTTCTTCCGTAGGTATCCGTTGGACTTTTGCACCGATGATCGACATTGCCCGTGATCCTCGCTGGGGGCGCATTGCCGAAGGATGTGGTGAAGACACTTACCTGACTTCTGTAATGGGAGCTGCCATGGTAGAAGGTTTTCAGGGAGATTCTTTGAATAGTCCCACTTCCATAGCTGCCTGTCCTAAACATTTTGTGGGCTATGGTGCAGCTGAAGGCGGACGTGACTATAATTCGACATTTATTCCTGAACGTCGCCTGCGTAATGTTTACTTGCCACCGTTTGAAGCGGCAACGAAAGCGGGTGCAGCTACGTTTATGACTTCCTTTAATGATAATGATGGGATACCCTCTACCGGAAATGCTTTCATATTGAAAGATGTGCTTCGTGGCGAGTGGGGATTTGATGGTTTGGTAGTGACAGACTGGGCTTCTGCCAGCGAAATGATAAGTCATGGTTTTGCTGCCGATTCTAAAGAGGTAGCCATGAAATCAGTGAATGCTGGGGTGGATATGGAAATGGTAAGTTATACCTTTGTAAAAGAATTGCCTGCATTGATAAAAGAAGGAAAGGTGAAAGAAAGCACCATTGATGAAGCCGTTCGTAATATATTGCGCGTCAAGTATCGTCTGGGATTGTTTGATGTTCCTTATGTAGATGAAAAGCAACCCTCTGTCATGTATGATCCTTCTCATCTGAAAGTAGCTAAGCAGGCTGCTGTAGAATCGGCTATCCTGTTGAAGAATGATAAAGAAGTACTGCCGTTACAGGAGTCTCTGAAAACCATTGCTGTGGTAGGACCTATGGCCAATGCGCCTTATGAACAATTGGGTACCTGGATCTTTGATGGTGAGAAAGCTCATACTCAGACACCACTGAATGCTATTAAGGAAATAGTTGGCGACAAAGTACAGGTGATTTATGAACCCGGATTAGCTTATAGCCGTGAGAAAAATCCGGCAGGCGTAGCAAAAGCTGCTGCTGTTGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAAGCCATTCTTTCGGGTGAAGCACACTGTCTGGCAGATTTGAATCTTCAGGGTGATCAAAGTGCTTTGATTACGGCTTTGGCTAAGACAGGTAAACCTGTAGTAACCATTGTGATGGCAGGTCGTCCGTTGACTATCGGTCAGGAAGTGGAAGAATCAACAGCTGTTCTTTATTCATTCCATCCGGGTACGATGGGTGGACCGGCATTGGCCGATCTGCTGTGGGGTAAGGCGGTTCCAAGTGGAAAAACACCGGTTACTTTCCCGAAGATGGTAGGACAAATTCCGGTATATTATGCTCATAACAATACCGGGCGGCCGGCTACACGTAATGAGGTGTTGCTGGATGATATTGCTGTTGAGGCTGGACAAACTTCATTGGGATGTACTTCTTTCTATATGGATGCCGGTTTTGATCCTTTATTCCCATTTGGCTATGGCTTGTCGTATACAACGTTCAAGTATAGTAATGTCAAACTTTCATCAGCGTCATTGAAGAAAGATGATGTATTGACTGTGACATTTGATCTGGAAAATACAGGTAAATATAAGGGGACGGAAGTTGCTCAATTGTATATACAAGATAAGGTTGGTTCTGTAACTCGTCCGGTGAAAGAACTGAAACGTTTTACTCGGGTAACCTTGAAACCGGGCGAGAAAAAGAATGTTTCGTTTGAACTACCCGTTAGTGAACTTGCATTTTGGAACATCGATATGGTGAAAGTTGTGGAACCCGGAGACTTTGGACTTTGGGTGGCAACAGACAGCCAATCGGGAGAAGAAGTTTTCTTTAAGGTGGTAGAT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,868 total letters Query= CMR200122.105 Length=2598 Score E Sequences producing significant alignments: (Bits) Value wt 3950 0.0 07G_G2_CMR200122.105_G08.seq 1440 0.0 02G_G2_CMR200122.105T7sh.seq 1164 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V Query_1 56 AATTCCCCTC-TAGAAATAATTTTG-TTTAACTTT-AAGAAGGAG-ATATACATATGCACCATCATCATCA-TCATTCTTC-TGGTGTAG 139 1 8 ........C.N.NC...N.T..N..N.........T.........C......N.N................C.........A.....N.. 97 D L W S H P Q F E K G T E N L Y F Q S N A T Q V Query_1 140 ATC-TG-TGG-TC-TCA-T--CCGCAG-TT-CGAAAA--GGGTACC-GAGAA-CCTG-TACTT-CCAAT-CCAATGCCACA-CAAG-T-G 210 0 1 ...-....-.-. 9 2 937 .-...-.TC..N...-..T......AA.......C.....A...N-.....T.....A....NN.....A....-.N. 865 1 98 ...C..C...N..A...A.--......A..-......--.......-.....-....N.....-.....-...........-....C.-. 176 A N K G S D A A T E K K V E S L L S R M T L E E K I G Query_1 211 GCAAATAAA--GGTAGCGATGCGGCAACCGAG--AAAAAAGTAGAGTCTC-TTTTATCC-AGAA-TGACCCTTGAAGAGAAAA-TCGGTC 292 0 10 .........--.....................--................-........-....-..................-...... 91 2 864 .........AG.....N............N..GA................C........C....A..................A...... 775 1 177 .........--.....T...............--................-........-....-..................-...... 258 Q M N Q I T S Y G N I E D M S S L I K K G E V G S I L N Query_1 293 AGATGAACCAGATTACCT-CTTAC-GGGAAT-ATTGAGGATATGAGTAGTTTAA--TTAAGAAAGGTGAA-GTCGGGTCTATCCTGAATG 376 0 92 ..................-.....-......-......................--..............-................... 175 2 774 ..................T.....C......T..N.......N.........N.AT..............A................... 685 1 259 ..................-.....-......-......................--..............-................... 342 E V D P V R I N A L Q R V A M E E S R L G I P L L I A R A V Query_1 377 AGGTGGATCCGGTA-CGTATTAATGCGTTGCAACGCGTAGCGATGGAGGAGTCCCGGTTGGGAATCCCTTTGTTGATAGCTCGCGCTGTT 465 0 176 ..............-......................................................................A.... 264 0 210 ............ 199 2 684 ..............A........................N....................A........................A.... 595 2 649 ............ 660 1 343 ..............-.............................................A........................A.... 431 1 377 ............ 366 I H G F K T I F P I P L G Q A A S F N P Q I A K D G A R V A Query_1 466 ATTCACGGGTTTAAAACCATTTTTCCCATCCCATTGGGACAAGCGGCTTCGTTCAATCCGCAGATTGCGAAAGACGGTGCACGGGTAGCG 555 0 265 .......................................................................................... 354 2 594 .......................................................................................... 505 1 432 .......................................................................................... 521 1 112 ..............-..... 130 A I E A S S V G I R W T F A P M I D I A R D P R W G R I A Query_1 556 GCTATTGAGGCTTCTTCCGTAGGTATCCGTTGGAC-TTTTGCACCGATGATCGACATTGCCCGTGATCCT-CGCTGGGGGCGCATTGCCG 643 0 355 ...................................-..................................-................... 442 2 504 ...................................-..................................-................... 417 1 522 ...................................T..................................C................... 611 E G C G E D T Y L T S V M G A A M V E G F Q G D S L N S P T Query_1 644 AAGGATGTGGTGAAGACA-CTTACCTGACTTCTGTAATGGGAGCTGCCATGGTAGAAGGTTTTCAGGGAGATTCTTTGAATAGTCCCACT 732 0 443 ..................-....................................................................... 531 2 416 ..................-....................................................................... 328 1 612 ..................C..N...............N.N............C...........N......................... 701 S I A A C P K H F V G Y G A A E G G R D Y N S T F I P E Query_1 733 TCCATAGCTGCCT-GTCCTAAACATTTTGTGGGCTATGGTGCAG-CTGAAGGCGGACGTGACTAT-AATTCGACAT-TTATTCCT-GAAC 817 0 532 .............-..............................-....................-..........-........-.... 616 2 327 .............-..............................-....................-..........-........-.... 243 1 702 .......N.....G..............................C..N.....N...........N..........C........N.... 791 R R L R N V Y L P P F E A A T K A G A A T F M T Query_1 818 G-TCG-CCTG--CGTAATG--TTTAC--TTG-CCACCGTTTG-AAGCGG-CAACGAAAG--CGGGTGCAG--CTACG--TTTATGACTTC 890 0 617 .-...-....--.......--.....--...-..........-......-.........--.........--.....--........... 689 2 242 .-...-....--.......--.....--...-..........-......-.........--.........--.....--........... 170 1 792 .T...C....GNGN....NGT....NCT...C..C.......C.N....C..N.T....GCG......CNGC.....GT..N........ 881 S F N D N D G I P S T G N A F I L K D V L R G E W G F D G L Query_1 891 C--TTTAATGATAATGATGGGATACCCTCTACCGGAAATGCTTTCATATTGAAAGATGTGCTTCGTGGCGAGTGGGGATTTGATGGTTTG 978 0 690 .--....................................................................................... 777 2 169 .--....................................................................................... 82 1 882 .CT..C..... 892 V V T D W A S A S E M I S H G F A A D S K E V A M K S V N A Query_1 979 GTAGTGACAGACTGGGCTTCTGCCAGCGAAATGATAAGTCATGGTTTTGCTGCCGATTCTAAAGAGGTAGCCATGAAATCAGTGAATGCT 1068 0 778 .......................................................................................... 867 2 81 ...................................................................-.....-....... 3 G V D M E M V S Y T F V K E L P A L I K E G K V K E S T I D Query_1 1069 GGGGTGGATATGGAAATGGTAAGTTATACCTTTGTAAAAGAATTGCCTGCATTGATAAAAGAAGGAAAGGTGAAAGAAAGCACCATTGAT 1158 0 868 .......................................................................................... 957 2 714 ............ 703 E A V R N I L R V K Y R L G L F D V P Y V D E K Q P S V M Y Query_1 1159 GAAGCCGTTCGTAATATATTGCGCGTCAAGTATCGTCTGGGATTGTTTGATGTTCCTTATGTAGATGAAAAGCAACCCTCTGTCATGTAT 1248 0 958 .......................................................................................... 1047 D P S H L K V A K Q A A V E S A I L L K N D K E V L P L Q E Query_1 1249 GATCCTTCTCATCTGAAAGTAGCTAAGCAGGCTGCTGTAGAATCGGCTATCCTGTTGAAGAATGATAAAGAAGTACTGCCGTTACAGGAG 1338 0 1048 .......................................................................................... 1137 S L K T I A V V G P M A N A P Y E Q L G T W I F D G E K A H Query_1 1339 TCTCTGAAAACCATTGCTGTGGTAGGACCTATGGCCAATGCGCCTTATGAACAATTGGGTACCTGGATCTTTGATGGTGAGAAAGCTCAT 1428 0 1138 .......................................................................................... 1227 T Q T P L N A I K E I V G D K V Q V I Y E P G L A Y S R E K Query_1 1429 ACTCAGACACCACTGAATGCTATTAAGGAAATAGTTGGCGACAAAGTACAGGTGATTTATGAACCCGGATTAGCTTATAGCCGTGAGAAA 1518 0 1228 .......................................................................................... 1317 N P A G V A K A A A V A A R A D V I L A F V G E E A I L S G Query_1 1519 AATCCGGCAGGCGTAGCAAAAGCTGCTGCTGTTGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAAGCCATTCTTTCGGGT 1608 0 1318 .......................................................................................... 1407 0 1363 ............ 1352 E A H C L A D L N L Q G D Q S A L I T A L A K T G K P V V T Query_1 1609 GAAGCACACTGTCTGGCAGATTTGAATCTTCAGGGTGATCAAAGTGCTTTGATTACGGCTTTGGCTAAGACAGGTAAACCTGTAGTAACC 1698 0 1408 .......................................................................................... 1497 I V M A G R P L T I G Q E V E E S T A V L Y S F H P G T M G Query_1 1699 ATTGTGATGGCAGGTCGTCCGTTGACTATCGGTCAGGAAGTGGAAGAATCAACAGCTGTTCTTTATTCATTCCATCCGGGTACGATGGGT 1788 0 1498 .......................................................................................... 1587 G P A L A D L L W G K A V P S G K T P V T F P K M V G Q I P Query_1 1789 GGACCGGCATTGGCCGATCTGCTGTGGGGTAAGGCGGTTCCAAGTGGAAAAACACCGGTTACTTTCCCGAAGATGGTAGGACAAATTCCG 1878 0 1588 .......................................................................................... 1677 V Y Y A H N N T G R P A T R N E V L L D D I A V E A G Q T S Query_1 1879 GTATATTATGCTCATAACAATACCGGGCGGCCGGCTACACGTAATGAGGTGTTGCTGGATGATATTGCTGTTGAGGCTGGACAAACTTCA 1968 0 1678 .......................................................................................... 1767 L G C T S F Y M D A G F D P L F P F G Y G L S Y T T F K Y S Query_1 1969 TTGGGATGTACTTCTTTCTATATGGATGCCGGTTTTGATCCTTTATTCCCATTTGGCTATGGCTTGTCGTATACAACGTTCAAGTATAGT 2058 0 1768 .......................................................................................... 1857 N V K L S S A S L K K D D V L T V T F D L E N T G K Y K G T Query_1 2059 AATGTCAAACTTTCATCAGCGTCATTGAAGAAAGATGATGTATTGACTGTGACATTTGATCTGGAAAATACAGGTAAATATAAGGGGACG 2148 0 1858 .......................................................................................... 1947 E V A Q L Y I Q D K V G S V T R P V K E L K R F T R V T L K Query_1 2149 GAAGTTGCTCAATTGTATATACAAGATAAGGTTGGTTCTGTAACTCGTCCGGTGAAAGAACTGAAACGTTTTACTCGGGTAACCTTGAAA 2238 0 1948 .......................................................................................... 2037 0 1971 ............ 1960 P G E K K N V S F E L P V S E L A F W N I D M V K V V E P G Query_1 2239 CCGGGCGAGAAAAAGAATGTTTCGTTTGAACTACCCGTTAGTGAACTTGCATTTTGGAACATCGATATGGTGAAAGTTGTGGAACCCGGA 2328 0 2038 .......................................................................................... 2127 D F G L W V A T D S Q S G E E V F F K V V D Query_1 2329 GACTTTGGACTTTGGGTGGCAACAGACAGCCAATCGGGAGAAGAAGTTTTCTTTAAGGTGGTAGAT 2394 0 2128 .................................................................. 2193 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12432677 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:02 AM Number of letters in database: 4,868 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200122.108 (CMR200122.20-750.P(750).E489A.pMCSG68)
TQVANKGSDAATEKKVESLLSRMTLEEKIGQMNQITSYGNIEDMSSLIKKGEVGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQIAKDGARVAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKDVLRGEWGFDGLVVTDWASASEMISHGFAADSKEVAMKSVNAGVDMEMVSYTFVKELPALIKEGKVKESTIDEAVRNILRVKYRLGLFDVPYVDEKQPSVMYDPSHLKVAKQAAVESAILLKNDKEVLPLQESLKTIAVVGPMANAPYEQLGTWIFDGEKAHTQTPLNAIKEIVGDKVQVIYEPGLAYSREKNPAGVAKAAAVAARADVILAFVGEEAILSGEAHCLADLNLQGDQSALITALAKTGKPVVTIVMAGRPLTIGQEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKTPVTFPKMVGQIPVYYAHNNTGRPATRNEVLLDDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNVKLSSASLKKDDVLTVTFDLENTGKYKGTEVAQLYIQDKVGSVTRPVKELKRFTRVTLKPGEKKNVSFELPVSELAFWNIDMVKVVEPGDFGLWVATDSQSGEEVFFKVVD
ACACAAGTGGCAAATAAAGGTAGCGATGCGGCAACCGAGAAAAAAGTAGAGTCTCTTTTATCCAGAATGACCCTTGAAGAGAAAATCGGTCAGATGAACCAGATTACCTCTTACGGGAATATTGAGGATATGAGTAGTTTAATTAAGAAAGGTGAAGTCGGGTCTATCCTGAATGAGGTGGATCCGGTACGTATTAATGCGTTGCAACGCGTAGCGATGGAGGAGTCCCGGTTGGGAATCCCTTTGTTGATAGCTCGCGATGTTATTCACGGGTTTAAAACCATTTTTCCCATCCCATTGGGACAAGCGGCTTCGTTCAATCCGCAGATTGCGAAAGACGGTGCACGGGTAGCGGCTATTGAGGCTTCTTCCGTAGGTATCCGTTGGACTTTTGCACCGATGATCGACATTGCCCGTGATCCTCGCTGGGGGCGCATTGCCGAAGGATGTGGTGAAGACACTTACCTGACTTCTGTAATGGGAGCTGCCATGGTAGAAGGTTTTCAGGGAGATTCTTTGAATAGTCCCACTTCCATAGCTGCCTGTCCTAAACATTTTGTGGGCTATGGTGCAGCTGAAGGCGGACGTGACTATAATTCGACATTTATTCCTGAACGTCGCCTGCGTAATGTTTACTTGCCACCGTTTGAAGCGGCAACGAAAGCGGGTGCAGCTACGTTTATGACTTCCTTTAATGATAATGATGGGATACCCTCTACCGGAAATGCTTTCATATTGAAAGATGTGCTTCGTGGCGAGTGGGGATTTGATGGTTTGGTAGTGACAGACTGGGCTTCTGCCAGCGAAATGATAAGTCATGGTTTTGCTGCCGATTCTAAAGAGGTAGCCATGAAATCAGTGAATGCTGGGGTGGATATGGAAATGGTAAGTTATACCTTTGTAAAAGAATTGCCTGCATTGATAAAAGAAGGAAAGGTGAAAGAAAGCACCATTGATGAAGCCGTTCGTAATATATTGCGCGTCAAGTATCGTCTGGGATTGTTTGATGTTCCTTATGTAGATGAAAAGCAACCCTCTGTCATGTATGATCCTTCTCATCTGAAAGTAGCTAAGCAGGCTGCTGTAGAATCGGCTATCCTGTTGAAGAATGATAAAGAAGTACTGCCGTTACAGGAGTCTCTGAAAACCATTGCTGTGGTAGGACCTATGGCCAATGCGCCTTATGAACAATTGGGTACCTGGATCTTTGATGGTGAGAAAGCTCATACTCAGACACCACTGAATGCTATTAAGGAAATAGTTGGCGACAAAGTACAGGTGATTTATGAACCCGGATTAGCTTATAGCCGTGAGAAAAATCCGGCAGGCGTAGCAAAAGCTGCTGCTGTTGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAAGCCATTCTTTCGGGTGAAGCACACTGTCTGGCAGATTTGAATCTTCAGGGTGATCAAAGTGCTTTGATTACGGCTTTGGCTAAGACAGGTAAACCTGTAGTAACCATTGTGATGGCAGGTCGTCCGTTGACTATCGGTCAGGAAGTGGAAGAATCAACAGCTGTTCTTTATTCATTCCATCCGGGTACGATGGGTGGACCGGCATTGGCCGATCTGCTGTGGGGTAAGGCGGTTCCAAGTGGAAAAACACCGGTTACTTTCCCGAAGATGGTAGGACAAATTCCGGTATATTATGCTCATAACAATACCGGGCGGCCGGCTACACGTAATGAGGTGTTGCTGGATGATATTGCTGTTGAGGCTGGACAAACTTCATTGGGATGTACTTCTTTCTATATGGATGCCGGTTTTGATCCTTTATTCCCATTTGGCTATGGCTTGTCGTATACAACGTTCAAGTATAGTAATGTCAAACTTTCATCAGCGTCATTGAAGAAAGATGATGTATTGACTGTGACATTTGATCTGGAAAATACAGGTAAATATAAGGGGACGGAAGTTGCTCAATTGTATATACAAGATAAGGTTGGTTCTGTAACTCGTCCGGTGAAAGAACTGAAACGTTTTACTCGGGTAACCTTGAAACCGGGCGAGAAAAAGAATGTTTCGTTTGAACTACCCGTTAGTGAACTTGCATTTTGGAACATCGATATGGTGAAAGTTGTGGAACCCGGAGACTTTGGACTTTGGGTGGCAACAGACAGCCAATCGGGAGAAGAAGTTTTCTTTAAGGTGGTAGAT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,929 total letters Query= CMR200122.108 Length=2598 Score E Sequences producing significant alignments: (Bits) Value wt 3947 0.0 07H_H2_CMR200122.108_H08.seq 1355 0.0 02H_H2_CMR200122.108_H06.seq 1000 0.0 T Q V A N K G S D A A T E K K V E S L L S R M T L E E K I G Query_1 202 ACACAAGTGGCAAATAAAGGTAGCGATGCGGCAACCGAGAAAAAAGTAGAGTCTCTTTTATCCAGAATGACCCTTGAAGAGAAAATCGGT 291 0 1 .......................................................................................... 90 Q M N Q I T S Y G N I E D M S S L I K K G E V G S I L N E V Query_1 292 CAGATGAACCAGATTACCTCTTACGGGAATATTGAGGATATGAGTAGTTTAATTAAGAAAGGTGAAGTCGGGTCTATCCTGAATGAGGTG 381 0 91 .......................................................................................... 180 D P V R I N A L Q R V A M E E S R L G I P L L I A R D V I H Query_1 382 GATCCGGTACGTATTAATGCGTTGCAACGCGTAGCGATGGAGGAGTCCCGGTTGGGAATCCCTTTGTTGATAGCTCGCGATGTTATTCAC 471 0 181 .......................................................................................... 270 0 210 ............ 199 G F K T I F P I P L G Q A A S F N P Q I A K D G A R V A A I Query_1 472 GGGTTTAAAACCATTTTTCCCATCCCATTGGGACAAGCGGCTTCGTTCAATCCGCAGATTGCGAAAGACGGTGCACGGGTAGCGGCTATT 561 0 271 .......................................................................................... 360 E A S S V G I R W T F A P M I D I A R D P R W G R I A E G C Query_1 562 GAGGCTTCTTCCGTAGGTATCCGTTGGACTTTTGCACCGATGATCGACATTGCCCGTGATCCTCGCTGGGGGCGCATTGCCGAAGGATGT 651 0 361 .......................................................................................... 450 G E D T Y L T S V M G A A M V E G F Q G D S L N S P T S I A Query_1 652 GGTGAAGACACTTACCTGACTTCTGTAATGGGAGCTGCCATGGTAGAAGGTTTTCAGGGAGATTCTTTGAATAGTCCCACTTCCATAGCT 741 0 451 .......................................................................................... 540 A C P K H F V G Y G A A E G G R D Y N S T F I P E R R L Query_1 742 GCCTGTCCTAAACATTTTGTGGGCTATGGTGCAGCTGAAGGCGGACGTGACTATAATTCGA-CATTTATT--CCTG-AACGTCGCCTG-C 826 0 541 .............................................................-........--....-...........-. 625 2 890 ......NN.....N..TC....N...........G. 855 R N V Y L P P F E A A T K A G A A T F M T S F N D Query_1 827 GT-AATGTTTACTTGCCACCG---TTTGAA-GCGGC--AACGAAA--GCGGGTG-CAGCT-ACGTTT-ATG-ACTTCCTTT-AATGATAA 902 0 626 ..-..................---......-.....--.......--.......-.....-......-...-.........-........ 701 2 854 .NN..............C..CCGT......N....NCA....G..TGN....G.G..AN.G......T...G.........T........ 765 N D G I P S T G N A F I L K D V L R G E W G F D G L V V T D Query_1 903 TGATGGGATACCCTCTA-CCGGAAA-TGCTTTCATATTGAAAGATGTGCTTCGTGGCGAGTGGGGATTTGATGGTTTGGTAGTGACAGAC 990 0 702 .................-.......-................................................................ 789 2 764 ......N.N........N.......G.......N...........N..N......................................... 675 W A S A S E M I S H G F A A D S K E V A M K S V N A G V D M Query_1 991 TGGGCTTCTGCCAGCGAAATGATAAGTCATGGTTTTGCTGCCGATTCTAAAGAGGTAGCCATGAAATCAGTGAATGCTGGGGTGGATATG 1080 0 790 .......................................................................................... 879 2 674 .......................................................................................... 585 E M V S Y T F V K E L P A L I K E G K V K E S T I D E A V R Query_1 1081 GAAATGGTAAGTTATACCTTTGTAAAAGAATTGCCTGCATTGATAAAAGAAGGAAAGGTGAAAGAAAGCACCATTGATGAAGCCGTTCGT 1170 0 880 .......................................................................................... 969 2 584 .............................................N............................................ 495 N I L R V K Y R L G L F D V P Y V D E K Q P S V M Y D P S H Query_1 1171 AATATATTGCGCGTCAAGTATCGTCTGGGATTGTTTGATGTTCCTTATGTAGATGAAAAGCAACCCTCTGTCATGTATGATCCTTCTCAT 1260 0 970 .......................................................................................... 1059 2 494 .......................................................................................... 405 L K V A K Q A A V E S A I L L K N D K E V L P L Q E S L K T Query_1 1261 CTGAAAGTAGCTAAGCAGGCTGCTGTAGAATCGGCTATCCTGTTGAAGAATGATAAAGAAGTACTGCCGTTACAGGAGTCTCTGAAAACC 1350 0 1060 .......................................................................................... 1149 2 404 .......................................................................................... 315 I A V V G P M A N A P Y E Q L G T W I F D G E K A H T Q Query_1 1351 ATTGCTGTGGTAGGACCTATGGCCAATGCGCCTTATGAACAATTG-GGTACC-TGGATCTTTGATGGTGAGAAAGC-TCATACT-CAGAC 1436 0 1150 .............................................-......-.......................-.......-..... 1235 2 314 .............................................-......-.......................-.......-..... 229 1 110 ...N...N......G.......................G.......T..... 161 T P L N A I K E I V G D K V Q V I Y E P G L A Y S R E K N P Query_1 1437 ACCACTGAATGCTATTAAGGAAATAGTTGGCGACAAAGTACAGGTGATTTATGAACCCGGATTAGCTTATAGCCGTGAGAAAAATCCGGC 1526 0 1236 .......................................................................................... 1325 2 228 .......................................................................................... 139 1 162 ..................................N....................................................... 251 A G V A K A A A V A A R A D V I L A F V G E E A I L S G A A Query_1 1527 AGGCGTAGCAAAAGCTGCTGCTGTTGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAAGCCATTCTTTCGGGTGCTGCA-C 1615 0 1326 ...................................................................................AA...-. 1414 0 1363 ............ 1352 2 138 ........................................................................................G. 49 2 101 ............ 112 1 252 ........................................................N..........N....................-. 340 1 289 ............ 278 H C L A D L N L Q G D Q S A L I T A L A K T G K P V V T I V Query_1 1616 ACTGTCTGGCAGATTTGAATCTTCAGGGTGATCAAAGTGCTTTGATTACGGCTTTGGCTAAGACAGGTAAACCTGTAGTAACCATTGTGA 1705 0 1415 .......................................................................................... 1504 2 48 .............-....................... 13 1 341 ....................................................................N..................... 430 M A G R P L T I G Q E V E E S T A V L Y S F H P G T M G G P Query_1 1706 TGGCAGGTCGTCCGTTGACTATCGGTCAGGAAGTGGAAGAATCAACAGCTGTTCTTTATTCATTCCATCCGGGTACGATGGGTGGACCGG 1795 0 1505 .......................................................................................... 1594 1 431 ...................................N......................N..N....N...............N....... 520 A L A D L L W G K A V P S G K T P V T F P K M V G Q I P V Y Query_1 1796 CATTGGCCGATCTGCTGTGGGGTAAGG-CGGTTCCAAGTGGAAAAACACCGGTTACTTTCCCGAAGATGGTAGGACAAATTCCGGTATAT 1884 0 1595 ...........................-.............................................................. 1683 1 521 .................N..N......A.....................................N.....N.................. 610 Y A H N N T G R P A T R N E V L L D D I A V E A G Q T S L Query_1 1885 TATGCTCATAACAATACCGGGCGGCCGGCTACACGTAATGAGGTGTTGCTGGATGATATTGCTGTTGAGGCTGGACAAACTTCATTGGG- 1973 0 1684 .........................................................................................- 1772 1 611 ........N..................N..N............C............N.N.......................NN.....C 700 G C T S F Y M D A G F D P L F P F G Y G L S Y T T F K Y Query_1 1974 -ATGTACTTCTTTCTATATGGATG-CCGGTTTT-GATCCTTTATTCCC-ATTTGG-CTATGGCTT-GTCG-TATACAACGTTCAAGTATA 2056 0 1773 -.......................-........-..............-......-.........-....-................... 1855 1 701 CNN.N.......N....N..N..NC........N......N.N.....N..N...C......N..N....C.....N......N.N.... 790 S N V K L S S A S L K K D D V L T V T F D L E N T G K Y K G Query_1 2057 G-TAATGTCAAACTTTCATCAGCGTCATTGAAGAAAGATGATGTATTGACTGTGACATTTGATCTGGAAAATACAGGTAAATATAAGGGG 2145 0 1856 .-........................................................................................ 1944 1 791 .C.... 796 T E V A Q L Y I Q D K V G S V T R P V K E L K R F T R V T L Query_1 2146 ACGGAAGTTGCTCAATTGTATATACAAGATAAGGTTGGTTCTGTAACTCGTCCGGTGAAAGAACTGAAACGTTTTACTCGGGTAACCTTG 2235 0 1945 .......................................................................................... 2034 0 1971 ............ 1960 K P G E K K N V S F E L P V S E L A F W N I D M V K V V E P Query_1 2236 AAACCGGGCGAGAAAAAGAATGTTTCGTTTGAACTACCCGTTAGTGAACTTGCATTTTGGAACATCGATATGGTGAAAGTTGTGGAACCC 2325 0 2035 .......................................................................................... 2124 G D F G L W V A T D S Q S G E E V F F K V V D Query_1 2326 GGAGACTTTGGACTTTGGGTGGCAACAGACAGCCAATCGGGAGAAGAAGTTTTCTTTAAGGTGGTAGAT 2394 0 2125 ..................................................................... 2193 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12590118 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:02 AM Number of letters in database: 4,929 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200122.112 (CMR200122.20-750.P(750).R588A.pMCSG68)
TQVANKGSDAATEKKVESLLSRMTLEEKIGQMNQITSYGNIEDMSSLIKKGEVGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQIAKDGARVAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKDVLRGEWGFDGLVVTDWASASEMISHGFAADSKEVAMKSVNAGVDMEMVSYTFVKELPALIKEGKVKESTIDEAVRNILRVKYRLGLFDVPYVDEKQPSVMYDPSHLKVAKQAAVESAILLKNDKEVLPLQESLKTIAVVGPMANAPYEQLGTWIFDGEKAHTQTPLNAIKEIVGDKVQVIYEPGLAYSREKNPAGVAKAAAVAARADVILAFVGEEAILSGEAHCLADLNLQGDQSALITALAKTGKPVVTIVMAGRPLTIGQEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKTPVTFPKMVGQIPVYYAHNNTGRPATRNEVLLDDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNVKLSSASLKKDDVLTVTFDLENTGKYKGTEVAQLYIQDKVGSVTRPVKELKRFTRVTLKPGEKKNVSFELPVSELAFWNIDMVKVVEPGDFGLWVATDSQSGEEVFFKVVD
ACACAAGTGGCAAATAAAGGTAGCGATGCGGCAACCGAGAAAAAAGTAGAGTCTCTTTTATCCAGAATGACCCTTGAAGAGAAAATCGGTCAGATGAACCAGATTACCTCTTACGGGAATATTGAGGATATGAGTAGTTTAATTAAGAAAGGTGAAGTCGGGTCTATCCTGAATGAGGTGGATCCGGTACGTATTAATGCGTTGCAACGCGTAGCGATGGAGGAGTCCCGGTTGGGAATCCCTTTGTTGATAGCTCGCGATGTTATTCACGGGTTTAAAACCATTTTTCCCATCCCATTGGGACAAGCGGCTTCGTTCAATCCGCAGATTGCGAAAGACGGTGCACGGGTAGCGGCTATTGAGGCTTCTTCCGTAGGTATCCGTTGGACTTTTGCACCGATGATCGACATTGCCCGTGATCCTCGCTGGGGGCGCATTGCCGAAGGATGTGGTGAAGACACTTACCTGACTTCTGTAATGGGAGCTGCCATGGTAGAAGGTTTTCAGGGAGATTCTTTGAATAGTCCCACTTCCATAGCTGCCTGTCCTAAACATTTTGTGGGCTATGGTGCAGCTGAAGGCGGACGTGACTATAATTCGACATTTATTCCTGAACGTCGCCTGCGTAATGTTTACTTGCCACCGTTTGAAGCGGCAACGAAAGCGGGTGCAGCTACGTTTATGACTTCCTTTAATGATAATGATGGGATACCCTCTACCGGAAATGCTTTCATATTGAAAGATGTGCTTCGTGGCGAGTGGGGATTTGATGGTTTGGTAGTGACAGACTGGGCTTCTGCCAGCGAAATGATAAGTCATGGTTTTGCTGCCGATTCTAAAGAGGTAGCCATGAAATCAGTGAATGCTGGGGTGGATATGGAAATGGTAAGTTATACCTTTGTAAAAGAATTGCCTGCATTGATAAAAGAAGGAAAGGTGAAAGAAAGCACCATTGATGAAGCCGTTCGTAATATATTGCGCGTCAAGTATCGTCTGGGATTGTTTGATGTTCCTTATGTAGATGAAAAGCAACCCTCTGTCATGTATGATCCTTCTCATCTGAAAGTAGCTAAGCAGGCTGCTGTAGAATCGGCTATCCTGTTGAAGAATGATAAAGAAGTACTGCCGTTACAGGAGTCTCTGAAAACCATTGCTGTGGTAGGACCTATGGCCAATGCGCCTTATGAACAATTGGGTACCTGGATCTTTGATGGTGAGAAAGCTCATACTCAGACACCACTGAATGCTATTAAGGAAATAGTTGGCGACAAAGTACAGGTGATTTATGAACCCGGATTAGCTTATAGCCGTGAGAAAAATCCGGCAGGCGTAGCAAAAGCTGCTGCTGTTGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAAGCCATTCTTTCGGGTGAAGCACACTGTCTGGCAGATTTGAATCTTCAGGGTGATCAAAGTGCTTTGATTACGGCTTTGGCTAAGACAGGTAAACCTGTAGTAACCATTGTGATGGCAGGTCGTCCGTTGACTATCGGTCAGGAAGTGGAAGAATCAACAGCTGTTCTTTATTCATTCCATCCGGGTACGATGGGTGGACCGGCATTGGCCGATCTGCTGTGGGGTAAGGCGGTTCCAAGTGGAAAAACACCGGTTACTTTCCCGAAGATGGTAGGACAAATTCCGGTATATTATGCTCATAACAATACCGGGCGGCCGGCTACACGTAATGAGGTGTTGCTGGATGATATTGCTGTTGAGGCTGGACAAACTTCATTGGGATGTACTTCTTTCTATATGGATGCCGGTTTTGATCCTTTATTCCCATTTGGCTATGGCTTGTCGTATACAACGTTCAAGTATAGTAATGTCAAACTTTCATCAGCGTCATTGAAGAAAGATGATGTATTGACTGTGACATTTGATCTGGAAAATACAGGTAAATATAAGGGGACGGAAGTTGCTCAATTGTATATACAAGATAAGGTTGGTTCTGTAACTCGTCCGGTGAAAGAACTGAAACGTTTTACTCGGGTAACCTTGAAACCGGGCGAGAAAAAGAATGTTTCGTTTGAACTACCCGTTAGTGAACTTGCATTTTGGAACATCGATATGGTGAAAGTTGTGGAACCCGGAGACTTTGGACTTTGGGTGGCAACAGACAGCCAATCGGGAGAAGAAGTTTTCTTTAAGGTGGTAGAT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,969 total letters Query= CMR200122.112 Length=2598 Score E Sequences producing significant alignments: (Bits) Value wt 3941 0.0 08A_A3_CMR200122.112_A09.seq 1440 0.0 03A_A3_CMR200122.112_A07.seq 374 2e-106 T Q V A N K G S D A A T E K K V E S L L S R M T L E E K I G Query_1 202 ACACAAGTGGCAAATAAAGGTAGCGATGCGGCAACCGAGAAAAAAGTAGAGTCTCTTTTATCCAGAATGACCCTTGAAGAGAAAATCGGT 291 0 1 .......................................................................................... 90 Q M N Q I T S Y G N I E D M S S L I K K G E V G S I L N E V Query_1 292 CAGATGAACCAGATTACCTCTTACGGGAATATTGAGGATATGAGTAGTTTAATTAAGAAAGGTGAAGTCGGGTCTATCCTGAATGAGGTG 381 0 91 .......................................................................................... 180 D P V R I N A L Q R V A M E E S R L G I P L L I A R D V I H Query_1 382 GATCCGGTACGTATTAATGCGTTGCAACGCGTAGCGATGGAGGAGTCCCGGTTGGGAATCCCTTTGTTGATAGCTCGCGATGTTATTCAC 471 0 181 .......................................................................................... 270 0 210 ............ 199 G F K T I F P I P L G Q A A S F N P Q I A K D G A R V A A I Query_1 472 GGGTTTAAAACCATTTTTCCCATCCCATTGGGACAAGCGGCTTCGTTCAATCCGCAGATTGCGAAAGACGGTGCACGGGTAGCGGCTATT 561 0 271 .......................................................................................... 360 E A S S V G I R W T F A P M I D I A R D P R W G R I A E G C Query_1 562 GAGGCTTCTTCCGTAGGTATCCGTTGGACTTTTGCACCGATGATCGACATTGCCCGTGATCCTCGCTGGGGGCGCATTGCCGAAGGATGT 651 0 361 .......................................................................................... 450 G E D T Y L T S V M G A A M V E G F Q G D S L N S P T S I A Query_1 652 GGTGAAGACACTTACCTGACTTCTGTAATGGGAGCTGCCATGGTAGAAGGTTTTCAGGGAGATTCTTTGAATAGTCCCACTTCCATAGCT 741 0 451 .......................................................................................... 540 A C P K H F V G Y G A A E G G R D Y N S T F I P E R R L R N Query_1 742 GCCTGTCCTAAACATTTTGTGGGCTATGGTGCAGCTGAAGGCGGACGTGACTATAATTCGACATTTATTCCTGAACGTCGCCTGCGTAAT 831 0 541 .......................................................................................... 630 V Y L P P F E A A T K A G A A T F M T S F N D N D G I P S T Query_1 832 GTTTACTTGCCACCGTTTGAAGCGGCAACGAAAGCGGGTGCAGCTACGTTTATGACTTCCTTTAATGATAATGATGGGATACCCTCTACC 921 0 631 .......................................................................................... 720 G N A F I L K D V L R G E W G F D G L V V T D W A S A S E M Query_1 922 GGAAATGCTTTCATATTGAAAGATGTGCTTCGTGGCGAGTGGGGATTTGATGGTTTGGTAGTGACAGACTGGGCTTCTGCCAGCGAAATG 1011 0 721 .......................................................................................... 810 I S H G F A A D S K E V A M K S V N A G V D M E M Query_1 1012 ATAAGTCATGGTTTT-GCTGCC-GATTCTAAAGAG---GTAGCC--ATGAAA--TCAGTGAAT--GCTGGGGT-GGATA-TGGAAATGG- 1087 0 811 ...............-......-............---......--......--.........--........-.....-.........- 886 2 1015 ......N......CN...T....A..AGG......CA......ATN.......AAGNN.....NN.....A.........G 935 V S Y T F V K E L P A L I K E G K V K E S T I D E A Query_1 1088 TAAG-TTATACCTTT-GTAAAAGAATTGCCTGC-ATTGATAAAA--GAAGG--AAAGGTG-AAAGAAAG--CACCATTGATGAAG-CCGT 1166 0 887 ....-..........-.................-..........--.....--.......-........--..............-.... 965 2 934 ....N..........T.N....A..........CN..N......AG.....GN......NN....N..TGC......N..N....G.... 845 V R N I L R V K Y R L G L F D V P Y V D E K Q P S V M Y Query_1 1167 TCGTAATA-TATTG-CGCGTCAA-GTATC-GTCTGGG-ATTGTTT-GATGTTCCTTATGTA-GATGAAAAGCAA-CCCTCTGTCATGTAT 1248 0 966 ........-.....-........-.....-.......-.......-...............-............-............... 1047 2 844 ........A.....G........A.....N...N...G.......T..N........N...A............A..N............ 755 D P S H L K V A K Q A A V E S A I L L K N D K E V L P L Q Query_1 1249 GATCCTTCTCATCTGAAAGTAGCTAAG-CAGGCTGCTGTAGAATCGGCTATCCTGTTGAA-GAATGATAAAGAAGTACTGCCGTTACAGG 1336 0 1048 ...........................-................................-............................. 1135 2 754 ...........................G................N...............A............................. 665 E S L K T I A V V G P M A N A P Y E Q L G T W I F D G E K A Query_1 1337 AGTCTCTGAAAACCATTGCTGTGGTAGGACCTATGGCCAATGCGCCTTATGAACAATTGGGTACCTGGATCTTTGATGGTGAGAAAGCTC 1426 0 1136 .......................................................................................... 1225 2 664 .......................................................................................... 575 H T Q T P L N A I K E I V G D K V Q V I Y E P G L A Y S R E Query_1 1427 ATACTCAGACACCACTGAATGCTATTAAGGAAATAGTTGGCGACAAAGTACAGGTGATTTATGAACCCGGATTAGCTTATAGCCGTGAGA 1516 0 1226 .......................................................................................... 1315 2 574 .................................................................................N........ 485 K N P A G V A K A A A V A A R A D V I L A F V G E E A I L S Query_1 1517 AAAATCCGGCAGGCGTAGCAAAAGCTGCTGCTGTTGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAAGCCATTCTTTCGG 1606 0 1316 .......................................................................................... 1405 0 1363 ............ 1352 2 484 .......................................................................................... 395 2 437 ............ 448 G E A H C L A D L N L Q G D Q S A L I T A L A K T G K P V V Query_1 1607 GTGAAGCACACTGTCTGGCAGATTTGAATCTTCAGGGTGATCAAAGTGCTTTGATTACGGCTTTGGCTAAGACAGGTAAACCTGTAGTAA 1696 0 1406 .......................................................................................... 1495 2 394 .......................................................................................... 305 T I V M A G R P L T I G Q E V E E S T A V L Y S F H P G T M Query_1 1697 CCATTGTGATGGCAGGTCGTCCGTTGACTATCGGTCAGGAAGTGGAAGAATCAACAGCTGTTCTTTATTCATTCCATCCGGGTACGATGG 1786 0 1496 .......................................................................................... 1585 2 304 .......................................................................................... 215 G G P A L A D L L W G K A V P S G K T P V T F P K M V G Q I Query_1 1787 GTGGACCGGCATTGGCCGATCTGCTGTGGGGTAAGGCGGTTCCAAGTGGAAAAACACCGGTTACTTTCCCGAAGATGGTAGGACAAATTC 1876 0 1586 .......................................................................................... 1675 2 214 .......................................................................................... 125 1 78 ...T... 84 P V Y Y A H N N T G A P A T R N E V L L D D I A V E Query_1 1877 CGG-TATATT--ATGC-TCA-TAACAA-TACCGGGGCTCCGGC-TA-CACGTAATGAGG-TGT-TGCTGGATGATATTGC-TGT-TGAGG 1954 0 1676 ...-......--....-...-......-.......CGG.....-..-............-...-................-...-..... 1753 2 124 ...-......--....-...-......-...............-..T............-...-................-...-..... 46 1 85 ...N..A.G.TA....A...G....N.C...............C..-.N....N.....C...N...N............A...C..... 173 A G Q T S L G C T S F Y M D A G F D P L F P F G Y G L S Y Query_1 1955 CTGGACAAA-CTTC-ATTGGGATGTACTTCTTT-CTATATGGATGCCGGTTTTGATCCTTTATTCCCATTTGGCTATGGC-TTGTCGTAT 2040 0 1754 .........-....-..................-..............................................-......... 1839 2 45 .........G....A.NA............N..AN...... 5 1 174 .........-....-N........C........-.....A.........N........N...........G........NA...A..N.. 260 T T F K Y S N V K L S S A S L K K D D V L T V T F D L E N Query_1 2041 ACAACGTTCAAGTATAGTAATGTCAAACTTTCATCAGCG-TCATTGAAGAAAGATGATGTATTGACTGTGACATTTGATCTGGAAAATAC 2129 0 1840 .......................................-.................................................. 1928 1 261 ..........N.......NN................T..N.................N..........N....................T 350 T G K Y K G T E V A Q L Y I Q D K V G S V T R P V K E L K Query_1 2130 AGGTAAATATAAGGGGACGGAAGTTGCTCAATTGTATATACAAGATAAGGTTGGTTCTGTAACTCGTCCGG-TGAAAGAACTGAAACG-- 2216 0 1929 .......................................................................-................-- 2015 0 1971 ............ 1960 1 351 .N..N.....NNT.N...N......N.N.N.....N..CN.N..N............N..CC....N....NN....N.NNNN.....CT 440 R F T R V T L K P G E K K N V S F E L P V S E L A F W N I D Query_1 2217 TTTTACTCGGG--TAACCTTGAAACCGGGCGAGAAAAAGAATGTTTCGTTTGAACTACCCGTTAGTGAACTTGCATTTTGGAACATCGAT 2304 0 2016 ...........--............................................................................. 2103 1 441 ...C.......TT..C...C...N..N..N.... 474 M V K V V E P G D F G L W V A T D S Q S G E E V F F K V V D Query_1 2305 ATGGTGAAAGTTGTGGAACCCGGAGACTTTGGACTTTGGGTGGCAACAGACAGCCAATCGGGAGAAGAAGTTTTCTTTAAGGTGGTAGAT 2394 0 2104 .......................................................................................... 2193 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12693358 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:02 AM Number of letters in database: 4,969 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200122.115 (CMR200122.20-750.P(750).E489Q.pMCSG68)
TQVANKGSDAATEKKVESLLSRMTLEEKIGQMNQITSYGNIEDMSSLIKKGEVGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQIAKDGARVAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKDVLRGEWGFDGLVVTDWASASEMISHGFAADSKEVAMKSVNAGVDMEMVSYTFVKELPALIKEGKVKESTIDEAVRNILRVKYRLGLFDVPYVDEKQPSVMYDPSHLKVAKQAAVESAILLKNDKEVLPLQESLKTIAVVGPMANAPYEQLGTWIFDGEKAHTQTPLNAIKEIVGDKVQVIYEPGLAYSREKNPAGVAKAAAVAARADVILAFVGEEAILSGEAHCLADLNLQGDQSALITALAKTGKPVVTIVMAGRPLTIGQEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKTPVTFPKMVGQIPVYYAHNNTGRPATRNEVLLDDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNVKLSSASLKKDDVLTVTFDLENTGKYKGTEVAQLYIQDKVGSVTRPVKELKRFTRVTLKPGEKKNVSFELPVSELAFWNIDMVKVVEPGDFGLWVATDSQSGEEVFFKVVD
ACACAAGTGGCAAATAAAGGTAGCGATGCGGCAACCGAGAAAAAAGTAGAGTCTCTTTTATCCAGAATGACCCTTGAAGAGAAAATCGGTCAGATGAACCAGATTACCTCTTACGGGAATATTGAGGATATGAGTAGTTTAATTAAGAAAGGTGAAGTCGGGTCTATCCTGAATGAGGTGGATCCGGTACGTATTAATGCGTTGCAACGCGTAGCGATGGAGGAGTCCCGGTTGGGAATCCCTTTGTTGATAGCTCGCGATGTTATTCACGGGTTTAAAACCATTTTTCCCATCCCATTGGGACAAGCGGCTTCGTTCAATCCGCAGATTGCGAAAGACGGTGCACGGGTAGCGGCTATTGAGGCTTCTTCCGTAGGTATCCGTTGGACTTTTGCACCGATGATCGACATTGCCCGTGATCCTCGCTGGGGGCGCATTGCCGAAGGATGTGGTGAAGACACTTACCTGACTTCTGTAATGGGAGCTGCCATGGTAGAAGGTTTTCAGGGAGATTCTTTGAATAGTCCCACTTCCATAGCTGCCTGTCCTAAACATTTTGTGGGCTATGGTGCAGCTGAAGGCGGACGTGACTATAATTCGACATTTATTCCTGAACGTCGCCTGCGTAATGTTTACTTGCCACCGTTTGAAGCGGCAACGAAAGCGGGTGCAGCTACGTTTATGACTTCCTTTAATGATAATGATGGGATACCCTCTACCGGAAATGCTTTCATATTGAAAGATGTGCTTCGTGGCGAGTGGGGATTTGATGGTTTGGTAGTGACAGACTGGGCTTCTGCCAGCGAAATGATAAGTCATGGTTTTGCTGCCGATTCTAAAGAGGTAGCCATGAAATCAGTGAATGCTGGGGTGGATATGGAAATGGTAAGTTATACCTTTGTAAAAGAATTGCCTGCATTGATAAAAGAAGGAAAGGTGAAAGAAAGCACCATTGATGAAGCCGTTCGTAATATATTGCGCGTCAAGTATCGTCTGGGATTGTTTGATGTTCCTTATGTAGATGAAAAGCAACCCTCTGTCATGTATGATCCTTCTCATCTGAAAGTAGCTAAGCAGGCTGCTGTAGAATCGGCTATCCTGTTGAAGAATGATAAAGAAGTACTGCCGTTACAGGAGTCTCTGAAAACCATTGCTGTGGTAGGACCTATGGCCAATGCGCCTTATGAACAATTGGGTACCTGGATCTTTGATGGTGAGAAAGCTCATACTCAGACACCACTGAATGCTATTAAGGAAATAGTTGGCGACAAAGTACAGGTGATTTATGAACCCGGATTAGCTTATAGCCGTGAGAAAAATCCGGCAGGCGTAGCAAAAGCTGCTGCTGTTGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAAGCCATTCTTTCGGGTGAAGCACACTGTCTGGCAGATTTGAATCTTCAGGGTGATCAAAGTGCTTTGATTACGGCTTTGGCTAAGACAGGTAAACCTGTAGTAACCATTGTGATGGCAGGTCGTCCGTTGACTATCGGTCAGGAAGTGGAAGAATCAACAGCTGTTCTTTATTCATTCCATCCGGGTACGATGGGTGGACCGGCATTGGCCGATCTGCTGTGGGGTAAGGCGGTTCCAAGTGGAAAAACACCGGTTACTTTCCCGAAGATGGTAGGACAAATTCCGGTATATTATGCTCATAACAATACCGGGCGGCCGGCTACACGTAATGAGGTGTTGCTGGATGATATTGCTGTTGAGGCTGGACAAACTTCATTGGGATGTACTTCTTTCTATATGGATGCCGGTTTTGATCCTTTATTCCCATTTGGCTATGGCTTGTCGTATACAACGTTCAAGTATAGTAATGTCAAACTTTCATCAGCGTCATTGAAGAAAGATGATGTATTGACTGTGACATTTGATCTGGAAAATACAGGTAAATATAAGGGGACGGAAGTTGCTCAATTGTATATACAAGATAAGGTTGGTTCTGTAACTCGTCCGGTGAAAGAACTGAAACGTTTTACTCGGGTAACCTTGAAACCGGGCGAGAAAAAGAATGTTTCGTTTGAACTACCCGTTAGTGAACTTGCATTTTGGAACATCGATATGGTGAAAGTTGTGGAACCCGGAGACTTTGGACTTTGGGTGGCAACAGACAGCCAATCGGGAGAAGAAGTTTTCTTTAAGGTGGTAGAT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 6,090 total letters Query= CMR200122.115 Length=2598 Score E Sequences producing significant alignments: (Bits) Value wt 3947 0.0 T Q V A N K G S D A A T E K K V E S L L S R M T L E E K I G Query_1 202 ACACAAGTGGCAAATAAAGGTAGCGATGCGGCAACCGAGAAAAAAGTAGAGTCTCTTTTATCCAGAATGACCCTTGAAGAGAAAATCGGT 291 0 1 .......................................................................................... 90 Q M N Q I T S Y G N I E D M S S L I K K G E V G S I L N E V Query_1 292 CAGATGAACCAGATTACCTCTTACGGGAATATTGAGGATATGAGTAGTTTAATTAAGAAAGGTGAAGTCGGGTCTATCCTGAATGAGGTG 381 0 91 .......................................................................................... 180 D P V R I N A L Q R V A M E E S R L G I P L L I A R D V I H Query_1 382 GATCCGGTACGTATTAATGCGTTGCAACGCGTAGCGATGGAGGAGTCCCGGTTGGGAATCCCTTTGTTGATAGCTCGCGATGTTATTCAC 471 0 181 .......................................................................................... 270 0 210 ............ 199 G F K T I F P I P L G Q A A S F N P Q I A K D G A R V A A I Query_1 472 GGGTTTAAAACCATTTTTCCCATCCCATTGGGACAAGCGGCTTCGTTCAATCCGCAGATTGCGAAAGACGGTGCACGGGTAGCGGCTATT 561 0 271 .......................................................................................... 360 E A S S V G I R W T F A P M I D I A R D P R W G R I A E G C Query_1 562 GAGGCTTCTTCCGTAGGTATCCGTTGGACTTTTGCACCGATGATCGACATTGCCCGTGATCCTCGCTGGGGGCGCATTGCCGAAGGATGT 651 0 361 .......................................................................................... 450 G E D T Y L T S V M G A A M V E G F Q G D S L N S P T S I A Query_1 652 GGTGAAGACACTTACCTGACTTCTGTAATGGGAGCTGCCATGGTAGAAGGTTTTCAGGGAGATTCTTTGAATAGTCCCACTTCCATAGCT 741 0 451 .......................................................................................... 540 A C P K H F V G Y G A A E G G R D Y N S T F I P E R R L R N Query_1 742 GCCTGTCCTAAACATTTTGTGGGCTATGGTGCAGCTGAAGGCGGACGTGACTATAATTCGACATTTATTCCTGAACGTCGCCTGCGTAAT 831 0 541 .......................................................................................... 630 V Y L P P F E A A T K A G A A T F M T S F N D N D G I P S T Query_1 832 GTTTACTTGCCACCGTTTGAAGCGGCAACGAAAGCGGGTGCAGCTACGTTTATGACTTCCTTTAATGATAATGATGGGATACCCTCTACC 921 0 631 .......................................................................................... 720 G N A F I L K D V L R G E W G F D G L V V T D W A S A S E M Query_1 922 GGAAATGCTTTCATATTGAAAGATGTGCTTCGTGGCGAGTGGGGATTTGATGGTTTGGTAGTGACAGACTGGGCTTCTGCCAGCGAAATG 1011 0 721 .......................................................................................... 810 I S H G F A A D S K E V A M K S V N A G V D M E M V S Y T F Query_1 1012 ATAAGTCATGGTTTTGCTGCCGATTCTAAAGAGGTAGCCATGAAATCAGTGAATGCTGGGGTGGATATGGAAATGGTAAGTTATACCTTT 1101 0 811 .......................................................................................... 900 V K E L P A L I K E G K V K E S T I D E A V R N I L R V K Y Query_1 1102 GTAAAAGAATTGCCTGCATTGATAAAAGAAGGAAAGGTGAAAGAAAGCACCATTGATGAAGCCGTTCGTAATATATTGCGCGTCAAGTAT 1191 0 901 .......................................................................................... 990 R L G L F D V P Y V D E K Q P S V M Y D P S H L K V A K Q A Query_1 1192 CGTCTGGGATTGTTTGATGTTCCTTATGTAGATGAAAAGCAACCCTCTGTCATGTATGATCCTTCTCATCTGAAAGTAGCTAAGCAGGCT 1281 0 991 .......................................................................................... 1080 A V E S A I L L K N D K E V L P L Q E S L K T I A V V G P M Query_1 1282 GCTGTAGAATCGGCTATCCTGTTGAAGAATGATAAAGAAGTACTGCCGTTACAGGAGTCTCTGAAAACCATTGCTGTGGTAGGACCTATG 1371 0 1081 .......................................................................................... 1170 A N A P Y E Q L G T W I F D G E K A H T Q T P L N A I K E I Query_1 1372 GCCAATGCGCCTTATGAACAATTGGGTACCTGGATCTTTGATGGTGAGAAAGCTCATACTCAGACACCACTGAATGCTATTAAGGAAATA 1461 0 1171 .......................................................................................... 1260 V G D K V Q V I Y E P G L A Y S R E K N P A G V A K A A A V Query_1 1462 GTTGGCGACAAAGTACAGGTGATTTATGAACCCGGATTAGCTTATAGCCGTGAGAAAAATCCGGCAGGCGTAGCAAAAGCTGCTGCTGTT 1551 0 1261 .......................................................................................... 1350 A A R A D V I L A F V G E E A I L S G Q A H C L A D L N L Q Query_1 1552 GCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAAGCCATTCTTTCGGGTCAGGCACACTGTCTGGCAGATTTGAATCTTCAG 1641 0 1351 .........................................................G.A.............................. 1440 0 1363 ............ 1352 G D Q S A L I T A L A K T G K P V V T I V M A G R P L T I G Query_1 1642 GGTGATCAAAGTGCTTTGATTACGGCTTTGGCTAAGACAGGTAAACCTGTAGTAACCATTGTGATGGCAGGTCGTCCGTTGACTATCGGT 1731 0 1441 .......................................................................................... 1530 Q E V E E S T A V L Y S F H P G T M G G P A L A D L L W G K Query_1 1732 CAGGAAGTGGAAGAATCAACAGCTGTTCTTTATTCATTCCATCCGGGTACGATGGGTGGACCGGCATTGGCCGATCTGCTGTGGGGTAAG 1821 0 1531 .......................................................................................... 1620 A V P S G K T P V T F P K M V G Q I P V Y Y A H N N T G R P Query_1 1822 GCGGTTCCAAGTGGAAAAACACCGGTTACTTTCCCGAAGATGGTAGGACAAATTCCGGTATATTATGCTCATAACAATACCGGGCGGCCG 1911 0 1621 .......................................................................................... 1710 A T R N E V L L D D I A V E A G Q T S L G C T S F Y M D A G Query_1 1912 GCTACACGTAATGAGGTGTTGCTGGATGATATTGCTGTTGAGGCTGGACAAACTTCATTGGGATGTACTTCTTTCTATATGGATGCCGGT 2001 0 1711 .......................................................................................... 1800 F D P L F P F G Y G L S Y T T F K Y S N V K L S S A S L K K Query_1 2002 TTTGATCCTTTATTCCCATTTGGCTATGGCTTGTCGTATACAACGTTCAAGTATAGTAATGTCAAACTTTCATCAGCGTCATTGAAGAAA 2091 0 1801 .......................................................................................... 1890 D D V L T V T F D L E N T G K Y K G T E V A Q L Y I Q D K V Query_1 2092 GATGATGTATTGACTGTGACATTTGATCTGGAAAATACAGGTAAATATAAGGGGACGGAAGTTGCTCAATTGTATATACAAGATAAGGTT 2181 0 1891 .......................................................................................... 1980 0 1971 ............ 1960 G S V T R P V K E L K R F T R V T L K P G E K K N V S F E L Query_1 2182 GGTTCTGTAACTCGTCCGGTGAAAGAACTGAAACGTTTTACTCGGGTAACCTTGAAACCGGGCGAGAAAAAGAATGTTTCGTTTGAACTA 2271 0 1981 .......................................................................................... 2070 P V S E L A F W N I D M V K V V E P G D F G L W V A T D S Q Query_1 2272 CCCGTTAGTGAACTTGCATTTTGGAACATCGATATGGTGAAAGTTGTGGAACCCGGAGACTTTGGACTTTGGGTGGCAACAGACAGCCAA 2361 0 2071 .......................................................................................... 2160 S G E E V F F K V V D Query_1 2362 TCGGGAGAAGAAGTTTTCTTTAAGGTGGTAGAT 2394 0 2161 ................................. 2193 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 15572880 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:02 AM Number of letters in database: 6,090 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200130.106 (CMR200130.32-851.P(851).E556A.pMCSG68)
SDSNVDFNKDWKFVLKDSAHYSYTSYVPGDEWKKVNLPHDWSVGLPYDSISGEGCVAFLQGGIGWYSKSFPTTISANQKCYIVFDGVYNNSEYWINGKKLGYHLSGYAPFYFDVTDYLNPNEDNRMTVRVDHSHYADSRWYTGSGIYRDVKMIVTDRLHIPVWGTFVTTPVVTDKYAKVNNQITVRNSYSEPRTAVVEIVYKDNKGNIAAFEVFSIKLNAGEEKIIDIVSEIKQPDLWSVEIPVLYTAETRIKNGDEVISENTVRFGIRTFHFDADKGFFLNGKNMKIKGVCLHHDAGIVGTAMIRDVWYRRLKTLKEGGCNAIRLSHNPGADEFLSLCDEIGLLVQEEFFDEWDYPKDKRLNMKETVEDYPTHGYCEHFQEWAERDLKNVMRRSRNHACIFQWSIGNEIEWTYTGCREATGFFGADSNGNYFWNQPPYSKEKIREMWKIQPKQAYDIGRTAQKLAAWTRQMDTTRVVTANCILPSISFETGYIDALDVAGFSYRRVMYDYAKKNYPDKPIMGTENLGQWHEWKAVIERDFVPGMFIWTGVDYLGESGSRLSRWPQKSIGCGLLDMCGYVKPSYDMMKSLWTDKPFIAIYSQTPDKSSYLQVKDGFTDKKGHEWDRRLWVWDDVNSHWNYQKGDSVIVEIYSNCDEVELFVNGKSMGKKYIDDFEDHIYKWAVQYKPGTITAKGKNKLGNTTTAIRTSGKEHSILLAVDKQSIAANGKDVLHVTAQLTDKKGNPVKTTEQMLKFNIDGEYRLLGIDNGNVKNVSPYQSKEIMTYQGRCMLMLQSTEKTSVLNISAETSELQSNKLTINIK
TCTGATTCAAATGTTGATTTCAATAAAGATTGGAAATTCGTACTGAAAGATTCTGCTCATTATTCATATACTTCTTATGTCCCTGGTGATGAATGGAAGAAAGTGAACCTGCCACACGACTGGAGTGTTGGTCTGCCTTACGACTCCATCTCTGGCGAAGGGTGTGTAGCTTTCCTTCAGGGAGGAATAGGATGGTATAGCAAATCATTTCCCACAACAATCAGCGCAAATCAGAAATGCTATATAGTGTTCGATGGAGTATATAATAATTCTGAGTATTGGATAAATGGCAAAAAACTTGGATATCATCTTTCGGGATATGCTCCTTTTTATTTTGATGTCACAGACTATCTCAATCCCAATGAGGATAACCGCATGACTGTAAGGGTCGACCACAGCCATTATGCCGACAGCAGATGGTACACCGGTTCAGGTATATACAGGGATGTGAAAATGATTGTAACCGACAGACTGCATATTCCGGTTTGGGGAACATTTGTCACTACTCCCGTGGTTACTGATAAATATGCTAAAGTAAACAACCAAATTACCGTGCGCAACAGTTACTCTGAACCCAGAACAGCTGTTGTTGAGATAGTGTATAAAGATAATAAAGGCAATATCGCAGCCTTTGAGGTCTTCAGTATAAAACTGAATGCTGGTGAGGAGAAAATTATCGACATCGTATCGGAGATAAAACAGCCGGATTTGTGGAGCGTCGAGATACCAGTCCTCTATACAGCCGAGACCCGTATTAAGAATGGCGATGAAGTCATTTCTGAAAACACTGTCAGGTTCGGTATACGAACATTCCACTTTGATGCAGACAAAGGTTTCTTCCTTAACGGAAAAAATATGAAGATAAAAGGAGTATGCCTGCATCATGATGCCGGTATAGTTGGCACAGCAATGATACGCGATGTGTGGTACCGACGTCTGAAAACCCTTAAGGAAGGAGGATGTAACGCCATCCGCCTTTCGCACAATCCGGGAGCGGATGAGTTTCTGTCTTTGTGCGATGAGATAGGTCTTCTGGTCCAGGAAGAGTTCTTCGATGAGTGGGATTATCCCAAAGATAAAAGGCTCAATATGAAGGAAACGGTAGAAGACTATCCTACTCATGGTTATTGTGAGCATTTCCAGGAATGGGCTGAAAGGGATTTGAAAAACGTAATGAGGAGAAGCCGTAATCATGCCTGTATCTTCCAGTGGAGTATAGGTAATGAAATAGAATGGACTTATACCGGATGCCGTGAGGCAACAGGTTTCTTTGGAGCCGATTCCAACGGTAATTACTTCTGGAACCAGCCTCCATACTCTAAAGAAAAAATCAGAGAAATGTGGAAAATCCAGCCTAAACAAGCATACGACATTGGTCGTACAGCGCAAAAATTAGCAGCATGGACACGCCAGATGGATACTACACGAGTGGTTACCGCCAACTGCATCCTGCCTTCCATAAGTTTTGAGACAGGATATATCGATGCACTTGATGTGGCTGGTTTCAGCTACAGACGCGTGATGTATGATTATGCTAAGAAGAATTATCCTGACAAACCTATAATGGGTACAGAAAATCTTGGTCAGTGGCACGAATGGAAGGCGGTGATTGAAAGAGATTTCGTTCCGGGTATGTTTATATGGACAGGAGTCGATTATCTGGGAGAAAGTGGAAGCCGCCTTTCAAGATGGCCTCAAAAGTCAATAGGATGTGGTCTCCTGGATATGTGCGGCTATGTGAAGCCTTCGTACGACATGATGAAATCATTGTGGACTGACAAGCCTTTTATTGCTATATATTCACAGACTCCAGACAAATCTTCGTATCTCCAGGTAAAAGATGGCTTTACTGATAAGAAAGGACATGAATGGGATAGAAGATTATGGGTTTGGGATGATGTAAACTCTCACTGGAATTATCAGAAAGGTGACTCGGTAATAGTAGAAATATATTCCAATTGTGATGAAGTGGAACTTTTCGTTAACGGCAAGTCGATGGGAAAGAAGTATATAGACGATTTTGAGGATCATATCTATAAATGGGCAGTTCAGTACAAGCCTGGCACTATTACCGCAAAAGGAAAAAATAAGTTAGGTAATACCACTACAGCTATAAGGACTTCAGGCAAAGAACATTCGATATTGCTAGCGGTTGACAAACAAAGTATCGCAGCAAATGGAAAGGATGTTCTGCATGTCACAGCCCAGCTTACAGACAAAAAAGGTAATCCTGTAAAGACAACAGAACAGATGCTTAAGTTCAACATCGATGGAGAGTACCGTCTGTTGGGTATAGACAATGGAAATGTAAAGAACGTATCTCCATATCAAAGCAAGGAGATTATGACATATCAGGGAAGATGTATGCTGATGCTTCAGTCAACAGAAAAAACATCGGTACTGAATATCAGTGCAGAAACAAGTGAATTACAGTCGAATAAACTAACAATTAATATAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,217 total letters Query= CMR200130.106 Length=2865 Score E Sequences producing significant alignments: (Bits) Value wt 4428 0.0 08C_C3_CMR200130.106_C09.seq 1938 0.0 03C_C3_CMR200130.106_C07.seq 509 5e-147 S D S N V D F N K D W K F V L K D S A H Y S Y T S Y V P G D Query_1 202 TCTGATTCAAATGTTGATTTCAATAAAGATTGGAAATTCGTACTGAAAGATTCTGCTCATTATTCATATACTTCTTATGTCCCTGGTGAT 291 0 1 .......................................................................................... 90 0 2012 ........... 2002 E W K K V N L P H D W S V G L P Y D S I S G E G C V A F L Q Query_1 292 GAATGGAAGAAAGTGAACCTGCCACACGACTGGAGTGTTGGTCTGCCTTACGACTCCATCTCTGGCGAAGGGTGTGTAGCTTTCCTTCAG 381 0 91 .......................................................................................... 180 G G I G W Y S K S F P T T I S A N Q K C Y I V F D G V Y N N Query_1 382 GGAGGAATAGGATGGTATAGCAAATCATTTCCCACAACAATCAGCGCAAATCAGAAATGCTATATAGTGTTCGATGGAGTATATAATAAT 471 0 181 .......................................................................................... 270 S E Y W I N G K K L G Y H L S G Y A P F Y F D V T D Y L N P Query_1 472 TCTGAGTATTGGATAAATGGCAAAAAACTTGGATATCATCTTTCGGGATATGCTCCTTTTTATTTTGATGTCACAGACTATCTCAATCCC 561 0 271 .......................................................................................... 360 N E D N R M T V R V D H S H Y A D S R W Y T G S G I Y R D V Query_1 562 AATGAGGATAACCGCATGACTGTAAGGGTCGACCACAGCCATTATGCCGACAGCAGATGGTACACCGGTTCAGGTATATACAGGGATGTG 651 0 361 .......................................................................................... 450 K M I V T D R L H I P V W G T F V T T P V V T D K Y A K V N Query_1 652 AAAATGATTGTAACCGACAGACTGCATATTCCGGTTTGGGGAACATTTGTCACTACTCCCGTGGTTACTGATAAATATGCTAAAGTAAAC 741 0 451 .......................................................................................... 540 2 1182 ..N...............-.....-...... 1154 N Q I T V R N S Y S E P R T A V V E I V Y K D N K G N I A A Query_1 742 AACCAAATTACCGTGCGCAACAGTTACTCTGAACCCAGAACAGCTGTTGTTGAGATAGTGTATAAAGATAATAAAGGCAATATCGCAGCC 831 0 541 .......................................................................................... 630 2 1153 NN.NN--..........GNNNN..-.......--...NNN.......N.........NNN-......................N...... 1070 F E V F S I K L N A G E E K I I D I V S E I K Q P D L W S V Query_1 832 TTTGAGGTCTTCAGTATAAAACTGAATGCTGGTGAGGAGAAAATTATCGACATCGTATCGGAGATAAAACAGCCGGATTTGTGGAGCGTC 921 0 631 .......................................................................................... 720 2 1069 ..-.......-.........-....N......N...........................................RA...N.-...... 984 E I P V L Y T A E T R I K N G D E V I S E N T V R F G I R T Query_1 922 GAGATACCAGTCCTCTATACAGCCGAGACCCGTATTAAGAATGGCGATGAAGTCATTTCTGAAAACACTGTCAGGTTCGGTATACGAACA 1011 0 721 .......................................................................................... 810 2 983 ...............................................................................N-......... 895 F H F D A D K G F F L N G K N M K I K G V C L H H D A G I Query_1 1012 TTCCACTTTGATGCAGACAAA-GGTTTCTTCCTTAACGGAAAAAATATGAAGATAAAAGGAGTATGCCTGCATCATGATGCCGGTATAGT 1100 0 811 .....................-.................................................................... 899 0 897 ...A..G............A..C... 872 0 1073 ........... 1083 2 894 .....................R.................................................................... 805 2 807 ...A..G............A..C... 832 2 631 ........... 621 V G T A M I R D V W Y R R L K T L K E G G C N A I R L S H N Query_1 1101 TGGCACAGCAATGATACGCGATGTGTGGTACCGACGTCTGAAAACCCTTAAGGAAGGAGGATGTAACGCCATCCGCCTTTCGCACAATCC 1190 0 900 .......................................................................................... 989 2 804 .......................................................................................... 715 P G A D E F L S L C D E I G L L V Q E E F F D E W D Y P K D Query_1 1191 GGGAGCGGATGAGTTTCTGTCTTTGTGCGATGAGATAGGTCTTCTGGTCCAGGAAGAGTTCTTCGATGAGTGGGATTATCCCAAAGATAA 1280 0 990 .......................................................................................... 1079 2 714 .......................................................................................... 625 K R L N M K E T V E D Y P T H G Y C E H F Q E W A E R D L K Query_1 1281 AAGGCTCAATATGAAGGAAACGGTAGAAGACTATCCTACTCATGGTTATTGTGAGCATTTCCAGGAATGGGCTGAAAGGGATTTGAAAAA 1370 0 1080 .......................................................................................... 1169 2 624 .......................................................................................... 535 N V M R R S R N H A C I F Q W S I G N E I E W T Y T G C R E Query_1 1371 CGTAATGAGGAGAAGCCGTAATCATGCCTGTATCTTCCAGTGGAGTATAGGTAATGAAATAGAATGGACTTATACCGGATGCCGTGAGGC 1460 0 1170 .......................................................................................... 1259 2 534 .......................................................................................... 445 A T G F F G A D S N G N Y F W N Q P P Y S K E K I R E M W K Query_1 1461 AACAGGTTTCTTTGGAGCCGATTCCAACGGTAATTACTTCTGGAACCAGCCTCCATACTCTAAAGAAAAAATCAGAGAAATGTGGAAAAT 1550 0 1260 .......................................................................................... 1349 2 444 .......................................................................................... 355 I Q P K Q A Y D I G R T A Q K L A A W T R Q M D T T R V V T Query_1 1551 CCAGCCTAAACAAGCATACGACATTGGTCGTACAGCGCAAAAATTAGCAGCATGG-ACACGCCAGATGGATACTACACGAGTGGTTACCG 1639 0 1350 .......................................................-.................................. 1438 2 354 .......................................................-.................................. 266 1 5 ........-............G.................................. 59 A N C I L P S I S F E T G Y I D A L D V A G F S Y R R V M Y Query_1 1640 CCAACTGCATCCTGCCTTCCATAAGTTTTGAGACAGGATATATCGATGCACTTG-ATGTGGCTGGTTTCAGCTACAGACGCGTGATGTAT 1728 0 1439 ......................................................-................................... 1527 2 265 ......................................................-................................... 177 1 60 .........C...NAAG.....................................C................................... 149 D Y A K K N Y P D K P I M G T A N L G Q W H E W K A V I E R Query_1 1729 GATTATGCTAAGAAGAATTATCCTGACAAACCTATAATGGGTACAGCTAATCTTGGTCAGTGGCACGAATGGAAGGCGGTGATTGAAAGA 1818 0 1528 ..............................................AA.......................................... 1617 2 176 .......................................................................................... 87 1 150 ..............................................................................N........... 239 D F V P G M F I W T G V D Y L G E S G S R L S R W P Q K S I Query_1 1819 GATTTCGTTCCGGGTATGTTTATATGGACAGGAGTCGATTATCTGGGAGAAAGTGGAAGCCGCCTTTCAAGATGGCCTCAAAAGTCAATA 1908 0 1618 .......................................................................................... 1707 2 86 ................................................................-.......-N......... 6 1 240 .......................................N.................TC.N.....GA.......N...TNCN.G..... 329 G C G L L D M C G Y V K P S Y D M M K S L W T D K P F I A Query_1 1909 GGATGTGGTCTCCTGGATAT-GTGCGGCTATGTGAAGCCTTCGTACGACATGATGAAATCATTGTGGACTGACAAGCCTTTTATTGCTAT 1997 0 1708 ....................-..................................................................... 1796 1 330 N..N....N.A..A....N.C...NN...T...N.N... 368 I Y S Q T P D K S S Y L Q V K D G F T D K K G H E W D R R L Query_1 1998 ATATTCACAGACTCCAGACAAATCTTCGTATCTCCAGGTAAAAGATGGCTTTACTGATAAGAAAGGACATGAATGGGATAGAAGATTATG 2087 0 1797 .......................................................................................... 1886 W V W D D V N S H W N Y Q K G D S V I V E I Y S N C D E V E Query_1 2088 GGTTTGGGATGATGTAAACTCTCACTGGAATTATCAGAAAGGTGACTCGGTAATAGTAGAAATATATTCCAATTGTGATGAAGTGGAACT 2177 0 1887 .......................................................................................... 1976 L F V N G K S M G K K Y I D D F E D H I Y K W A V Q Y K P G Query_1 2178 TTTCGTTAACGGCAAGTCGATGGGAAAGAAGTATATAGACGATTTTGAGGATCATATCTATAAATGGGCAGTTCAGTACAAGCCTGGCAC 2267 0 1977 .......................................................................................... 2066 0 76 ........... 66 T I T A K G K N K L G N T T T A I R T S G K E H S I L L A V Query_1 2268 TATTACCGCAAAAGGAAAAAATAAGTTAGGTAATACCACTACAGCTATAAGGACTTCAGGCAAAGAACATTCGATATTGCTAGCGGTTGA 2357 0 2067 .......................................................................................... 2156 D K Q S I A A N G K D V L H V T A Q L T D K K G N P V K T T Query_1 2358 CAAACAAAGTATCGCAGCAAATGGAAAGGATGTTCTGCATGTCACAGCCCAGCTTACAGACAAAAAAGGTAATCCTGTAAAGACAACAGA 2447 0 2157 .......................................................................................... 2246 E Q M L K F N I D G E Y R L L G I D N G N V K N V S P Y Q S Query_1 2448 ACAGATGCTTAAGTTCAACATCGATGGAGAGTACCGTCTGTTGGGTATAGACAATGGAAATGTAAAGAACGTATCTCCATATCAAAGCAA 2537 0 2247 .......................................................................................... 2336 K E I M T Y Q G R C M L M L Q S T E K T S V L N I S A E T S Query_1 2538 GGAGATTATGACATATCAGGGAAGATGTATGCTGATGCTTCAGTCAACAGAAAAAACATCGGTACTGAATATCAGTGCAGAAACAAGTGA 2627 0 2337 .......................................................................................... 2426 E L Q S N K L T I N I K Query_1 2628 ATTACAGTCGAATAAACTAACAATTAATATAAAA 2661 0 2427 .................................. 2460 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 14712768 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:02 AM Number of letters in database: 5,217 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200130.108 (CMR200130.32-851.P(851).E440Q.pMCSG68)
SDSNVDFNKDWKFVLKDSAHYSYTSYVPGDEWKKVNLPHDWSVGLPYDSISGEGCVAFLQGGIGWYSKSFPTTISANQKCYIVFDGVYNNSEYWINGKKLGYHLSGYAPFYFDVTDYLNPNEDNRMTVRVDHSHYADSRWYTGSGIYRDVKMIVTDRLHIPVWGTFVTTPVVTDKYAKVNNQITVRNSYSEPRTAVVEIVYKDNKGNIAAFEVFSIKLNAGEEKIIDIVSEIKQPDLWSVEIPVLYTAETRIKNGDEVISENTVRFGIRTFHFDADKGFFLNGKNMKIKGVCLHHDAGIVGTAMIRDVWYRRLKTLKEGGCNAIRLSHNPGADEFLSLCDEIGLLVQEEFFDEWDYPKDKRLNMKETVEDYPTHGYCEHFQEWAERDLKNVMRRSRNHACIFQWSIGNEIEWTYTGCREATGFFGADSNGNYFWNQPPYSKEKIREMWKIQPKQAYDIGRTAQKLAAWTRQMDTTRVVTANCILPSISFETGYIDALDVAGFSYRRVMYDYAKKNYPDKPIMGTENLGQWHEWKAVIERDFVPGMFIWTGVDYLGESGSRLSRWPQKSIGCGLLDMCGYVKPSYDMMKSLWTDKPFIAIYSQTPDKSSYLQVKDGFTDKKGHEWDRRLWVWDDVNSHWNYQKGDSVIVEIYSNCDEVELFVNGKSMGKKYIDDFEDHIYKWAVQYKPGTITAKGKNKLGNTTTAIRTSGKEHSILLAVDKQSIAANGKDVLHVTAQLTDKKGNPVKTTEQMLKFNIDGEYRLLGIDNGNVKNVSPYQSKEIMTYQGRCMLMLQSTEKTSVLNISAETSELQSNKLTINIK
TCTGATTCAAATGTTGATTTCAATAAAGATTGGAAATTCGTACTGAAAGATTCTGCTCATTATTCATATACTTCTTATGTCCCTGGTGATGAATGGAAGAAAGTGAACCTGCCACACGACTGGAGTGTTGGTCTGCCTTACGACTCCATCTCTGGCGAAGGGTGTGTAGCTTTCCTTCAGGGAGGAATAGGATGGTATAGCAAATCATTTCCCACAACAATCAGCGCAAATCAGAAATGCTATATAGTGTTCGATGGAGTATATAATAATTCTGAGTATTGGATAAATGGCAAAAAACTTGGATATCATCTTTCGGGATATGCTCCTTTTTATTTTGATGTCACAGACTATCTCAATCCCAATGAGGATAACCGCATGACTGTAAGGGTCGACCACAGCCATTATGCCGACAGCAGATGGTACACCGGTTCAGGTATATACAGGGATGTGAAAATGATTGTAACCGACAGACTGCATATTCCGGTTTGGGGAACATTTGTCACTACTCCCGTGGTTACTGATAAATATGCTAAAGTAAACAACCAAATTACCGTGCGCAACAGTTACTCTGAACCCAGAACAGCTGTTGTTGAGATAGTGTATAAAGATAATAAAGGCAATATCGCAGCCTTTGAGGTCTTCAGTATAAAACTGAATGCTGGTGAGGAGAAAATTATCGACATCGTATCGGAGATAAAACAGCCGGATTTGTGGAGCGTCGAGATACCAGTCCTCTATACAGCCGAGACCCGTATTAAGAATGGCGATGAAGTCATTTCTGAAAACACTGTCAGGTTCGGTATACGAACATTCCACTTTGATGCAGACAAAGGTTTCTTCCTTAACGGAAAAAATATGAAGATAAAAGGAGTATGCCTGCATCATGATGCCGGTATAGTTGGCACAGCAATGATACGCGATGTGTGGTACCGACGTCTGAAAACCCTTAAGGAAGGAGGATGTAACGCCATCCGCCTTTCGCACAATCCGGGAGCGGATGAGTTTCTGTCTTTGTGCGATGAGATAGGTCTTCTGGTCCAGGAAGAGTTCTTCGATGAGTGGGATTATCCCAAAGATAAAAGGCTCAATATGAAGGAAACGGTAGAAGACTATCCTACTCATGGTTATTGTGAGCATTTCCAGGAATGGGCTGAAAGGGATTTGAAAAACGTAATGAGGAGAAGCCGTAATCATGCCTGTATCTTCCAGTGGAGTATAGGTAATGAAATAGAATGGACTTATACCGGATGCCGTGAGGCAACAGGTTTCTTTGGAGCCGATTCCAACGGTAATTACTTCTGGAACCAGCCTCCATACTCTAAAGAAAAAATCAGAGAAATGTGGAAAATCCAGCCTAAACAAGCATACGACATTGGTCGTACAGCGCAAAAATTAGCAGCATGGACACGCCAGATGGATACTACACGAGTGGTTACCGCCAACTGCATCCTGCCTTCCATAAGTTTTGAGACAGGATATATCGATGCACTTGATGTGGCTGGTTTCAGCTACAGACGCGTGATGTATGATTATGCTAAGAAGAATTATCCTGACAAACCTATAATGGGTACAGAAAATCTTGGTCAGTGGCACGAATGGAAGGCGGTGATTGAAAGAGATTTCGTTCCGGGTATGTTTATATGGACAGGAGTCGATTATCTGGGAGAAAGTGGAAGCCGCCTTTCAAGATGGCCTCAAAAGTCAATAGGATGTGGTCTCCTGGATATGTGCGGCTATGTGAAGCCTTCGTACGACATGATGAAATCATTGTGGACTGACAAGCCTTTTATTGCTATATATTCACAGACTCCAGACAAATCTTCGTATCTCCAGGTAAAAGATGGCTTTACTGATAAGAAAGGACATGAATGGGATAGAAGATTATGGGTTTGGGATGATGTAAACTCTCACTGGAATTATCAGAAAGGTGACTCGGTAATAGTAGAAATATATTCCAATTGTGATGAAGTGGAACTTTTCGTTAACGGCAAGTCGATGGGAAAGAAGTATATAGACGATTTTGAGGATCATATCTATAAATGGGCAGTTCAGTACAAGCCTGGCACTATTACCGCAAAAGGAAAAAATAAGTTAGGTAATACCACTACAGCTATAAGGACTTCAGGCAAAGAACATTCGATATTGCTAGCGGTTGACAAACAAAGTATCGCAGCAAATGGAAAGGATGTTCTGCATGTCACAGCCCAGCTTACAGACAAAAAAGGTAATCCTGTAAAGACAACAGAACAGATGCTTAAGTTCAACATCGATGGAGAGTACCGTCTGTTGGGTATAGACAATGGAAATGTAAAGAACGTATCTCCATATCAAAGCAAGGAGATTATGACATATCAGGGAAGATGTATGCTGATGCTTCAGTCAACAGAAAAAACATCGGTACTGAATATCAGTGCAGAAACAAGTGAATTACAGTCGAATAAACTAACAATTAATATAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,818 total letters Query= CMR200130.108 Length=2865 Score E Sequences producing significant alignments: (Bits) Value wt 4428 0.0 08D_D3_CMR200130.108_D09.seq 1891 0.0 03D_D3_CMR200130.108_D07.seq 1707 0.0 S D S N V D F N K D W K F V L K D S A H Y S Y T S Y V P G D Query_1 202 TCTGATTCAAATGTTGATTTCAATAAAGATTGGAAATTCGTACTGAAAGATTCTGCTCATTATTCATATACTTCTTATGTCCCTGGTGAT 291 0 1 .......................................................................................... 90 0 2012 ........... 2002 1 868 ........... 858 E W K K V N L P H D W S V G L P Y D S I S G E G C V A F L Q Query_1 292 GAATGGAAGAAAGTGAACCTGCCACACGACTGGAGTGTTGGTCTGCCTTACGACTCCATCTCTGGCGAAGGGTGTGTAGCTTTCCTTCAG 381 0 91 .......................................................................................... 180 2 1147 ....N......... 1134 G G I G W Y S K S F P T T I S A N Q K C Y I V F D G V Y N Query_1 382 GGAGGAATAGGATGGTATAGC-AAATCATTTCCCACAACAATCAGCGC-AAATCAG-AAATGCTATATAGTGTTCGATGGAGTATATAAT 468 0 181 .....................-..........................-.......-................................. 267 2 1133 ...CNN-..............C..N.....N...C--........N..N.......G................-................ 1048 N S E Y W I N G K K L G Y H L S G Y A P F Y F D V T D Y L N Query_1 469 AATTCTGAGTATTGGATAAATGGCAAAAAACTTGGATATCATCTTTCGGGATATGCTCCTTTTTATTTTGATGTCACAGACTATCTCAAT 558 0 268 .......................................................................................... 357 2 1047 ...............NAT..N...C.............................................CN.NAN.............N 958 P N E D N R M T V R V D H S H Y A D S R W Y T G S G I Y R D Query_1 559 CCCAATGAGGATAACCGCATGACTGTAAGGGTCGACCACAGCCATTATGCCGACAGCAGATGGTACACCGGTTCAGGTATATACAGGGAT 648 0 358 .......................................................................................... 447 2 957 N......................................................................................... 868 V K M I V T D R L H I P V W G T F V T T P V V T D K Y A K Query_1 649 GTGAAAA-TGATTGTAACCGACAGACTGCATATTCCGGTTTGGGGAACATTTGTCACTACTCCCGTGGTTACTGATAAATATGCTAAAGT 737 0 448 .......-.................................................................................. 536 2 867 .......A........-.........................................N............................... 779 V N N Q I T V R N S Y S E P R T A V V E I V Y K D N K G N I Query_1 738 AAACAACCAAATTACCGTGCGCAACAGTTACTCTGAACCCAGAACAGCTGTTGTTGAGATAGTGTATAAAGATAATAAAGGCAATATCGC 827 0 537 .......................................................................................... 626 2 778 .......................................................................................... 689 A A F E V F S I K L N A G E E K I I D I V S E I K Q P D L W Query_1 828 AGCCTTTGAGGTCTTCAGTATAAAACTGAATGCTGGTGAGGAGAAAATTATCGACATCGTATCGGAGATAAAACAGCCGGATTTGTGGAG 917 0 627 .......................................................................................... 716 2 688 .......................................................................................... 599 S V E I P V L Y T A E T R I K N G D E V I S E N T V R F G I Query_1 918 CGTCGAGATACCAGTCCTCTATACAGCCGAGACCCGTATTAAGAATGGCGATGAAGTCATTTCTGAAAACACTGTCAGGTTCGGTATACG 1007 0 717 .......................................................................................... 806 2 598 .......................................................................................... 509 R T F H F D A D K G F F L N G K N M K I K G V C L H H D A G Query_1 1008 AACATTCCACTTTGATGCAGACAAAGGTTTCTTCCTTAACGGAAAAAATATGAAGATAAAAGGAGTATGCCTGCATCATGATGCCGGTAT 1097 0 807 .......................................................................................... 896 0 897 ...A..G............A..C.. 873 0 1073 ........... 1083 2 508 .......................................................................................... 419 2 418 ...A..G............A..C.. 442 I V G T A M I R D V W Y R R L K T L K E G G C N A I R L S H Query_1 1098 AGTTGGCACAGCAATGATACGCGATGTGTGGTACCGACGTCTGAAAACCCTTAAGGAAGGAGGATGTAACGCCATCCGCCTTTCGCACAA 1187 0 897 .......................................................................................... 986 0 872 . 872 2 418 .......................................................................................... 329 2 443 . 443 N P G A D E F L S L C D E I G L L V Q E E F F D E W D Y P K Query_1 1188 TCCGGGAGCGGATGAGTTTCTGTCTTTGTGCGATGAGATAGGTCTTCTGGTCCAGGAAGAGTTCTTCGATGAGTGGGATTATCCCAAAGA 1277 0 987 .......................................................................................... 1076 2 328 .......................................................................................... 239 2 456 .... 453 D K R L N M K E T V E D Y P T H G Y C E H F Q E W A E R D L Query_1 1278 TAAAAGGCTCAATATGAAGGAAACGGTAGAAGACTATCCTACTCATGGTTATTGTGAGCATTTCCAGGAAT-GGGCTGAAAGGGATTTGA 1366 0 1077 .......................................................................-.................. 1165 2 238 .......................................................................-.................. 150 2 452 ....... 446 1 1 ......T.....NN.....N..GN. 25 K N V M R R S R N H A C I F Q W S I G N Q I E W T Y T G C R Query_1 1367 AAAACGTAATGAGGAGAAGCCGTAATCATGCCTGTATCTTCCAGTGGAGTATAGGTAATCAGATAGAATGGACTTATACCGGATGCCGTG 1456 0 1166 ...........................................................G.A............................ 1255 2 149 .......................................................................................... 60 1 26 C.--...................................................................................... 113 E A T G F F G A D S N G N Y F W N Q P P Y S K E K I R E M W Query_1 1457 AGGCAACAGGTTTCTTTGGAGCCGATTCCAACGGTAATTACTTCTGGAACCAGCCTCCATACTCTAAAGAAAAAATCAGAGAAATGTGGA 1546 0 1256 .......................................................................................... 1345 2 59 .....................................-..... 18 1 114 .......................................................................................... 203 K I Q P K Q A Y D I G R T A Q K L A A W T R Q M D T T R V V Query_1 1547 AAATCCAGCCTAAACAAGCATACGACATTGGTCGTACAGCGCAAAAATTAGCAGCATGGACACGCCAGATGGATACTACACGAGTGGTTA 1636 0 1346 .......................................................................................... 1435 1 204 .......................................................................................... 293 T A N C I L P S I S F E T G Y I D A L D V A G F S Y R R V M Query_1 1637 CCGCCAACTGCATCCTGCCTTCCATAAGTTTTGAGACAGGATATATCGATGCACTTGATGTGGCTGGTTTCAGCTACAGACGCGTGATGT 1726 0 1436 .......................................................................................... 1525 1 294 ...........................N........................N..................................... 383 Y D Y A K K N Y P D K P I M G T E N L G Q W H E W K A V I E Query_1 1727 ATGATTATGCTAAGAAGAATTATCCTGACAAACCTATAATGGGTACAGAAAATCTTGGTCAGTGGCACGAATGGAAGGCGGTGATTGAAA 1816 0 1526 .......................................................................................... 1615 1 384 .......................................................................................... 473 R D F V P G M F I W T G V D Y L G E S G S R L S R W P Q K S Query_1 1817 GAGATTTCGTTCCGGGTATGTTTATATGGACAGGAGTCGATTATCTGGGAGAAAGTGGAAGCCGCCTTTCAAGATGGCCTCAAAAGTCAA 1906 0 1616 .......................................................................................... 1705 1 474 .......................................................................................... 563 I G C G L L D M C G Y V K P S Y D M M K S L W T D K P F I A Query_1 1907 TAGGATGTGGTCTCCTGGATATGTGCGGCTATGTGAAGCCTTCGTACGACATGATGAAATCATTGTGGACTGACAAGCCTTTTATTGCTA 1996 0 1706 .......................................................................................... 1795 1 564 .......................................................N.................................. 653 I Y S Q T P D K S S Y L Q V K D G F T D K K G H E W D R R L Query_1 1997 TATATTCACAGACTCCAGACAAATCTTCGTATCTCCAGGTAAAAGATGGC-TTTACTGATAAGAAAGGACATGAATGGGATAGAAGATTA 2085 0 1796 ..................................................-....................................... 1884 1 654 ..................................................T....................................... 743 W V W D D V N S H W N Y Q K G D S V I V E I Y S N C D E V E Query_1 2086 TGGGTTTGGGATGATGTAAACTCTCACTGGAATTATCAGAAAGGTGACTCGGTAATAGTAGAAATATATTCCAATTGTGATGAAGTGGAA 2175 0 1885 .......................................................................................... 1974 1 744 ......................................................................................-... 832 L F V N G K S M G K K Y I D D F E D H I Y K W A V Q Y K P G Query_1 2176 CTTTTCGTTAACGGCAAGTCGATGGGAAAGAAGTATATAGACGATTTTGAGGATCATATCTATAAATGGGCAGTTCAGTACAAGCCTGGC 2265 0 1975 .......................................................................................... 2064 0 76 ........... 66 1 833 ......--.T.................................T.............N................NN.-N......N.... 919 T I T A K G K N K L G N T T T A I R T S G K E H S I L L A Query_1 2266 ACTA-TTACCGCAAAAGGAAAAAATAAGTTAGGTAATACCACTACAGCTA-TAAGGACTTCAGGCAAAGAACATTCGATATTGCTAGCGG 2353 0 2065 ....-.............................................-....................................... 2152 1 920 ....T..........--.G.........-........N............T..C...........-.....Y.....W.-N.......N. 1004 V D K Q S I A A N G K D V L H V T A Q L T D K K G N P V K T Query_1 2354 TTGACAAACAAAGTATCGCAGCAAATGGAAAGGATGTTCTGCATGTCACAGCCCAGCTTACAGACAAAAAAGGTAATCCTGTAAAGACAA 2443 0 2153 .......................................................................................... 2242 1 1005 .....--.TC.....-N.......W.-.......N..N........-N....N............-T...N....T........----NN 1084 T E Q M L K F N I D G E Y R L L G I D N G N V K N V S P Y Q Query_1 2444 CAGAACAGATGCTTAAGTTCAACATCGATGGAGAGTACCGTCTGTTGGGTATAGACAATGGAAATGTAAAGAACGTATCTCCATATCAAA 2533 0 2243 .......................................................................................... 2332 1 1085 N.NN..C..ANNN.T.N......T....N...-...N.....NN.... 1131 S K E I M T Y Q G R C M L M L Q S T E K T S V L N I S A E T Query_1 2534 GCAAGGAGATTATGACATATCAGGGAAGATGTATGCTGATGCTTCAGTCAACAGAAAAAACATCGGTACTGAATATCAGTGCAGAAACAA 2623 0 2333 .......................................................................................... 2422 S E L Q S N K L T I N I K Query_1 2624 GTGAATTACAGTCGAATAAACTAACAATTAATATAAAA 2661 0 2423 ...................................... 2460 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 13576416 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:02 AM Number of letters in database: 4,818 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200135.107 (CMR200135.22-812.P(812).Y483A.pMCSG68)
QRHEQLLETGWKFHKGETNGAETVSFNDSQWESVCIPHDWAIYGPFDRNNDLQNVAITQNLEKQASVKTGRTGGLPYVGVGWYRTRFDADPDKKTTLVFDGAMSEARVYVNGKEACFWPFGYNSFHCDITELLHKEGKDNVLAVRLENRPQSSRWYPGAGLYRNVHLITAEKIHVPVWGTQVTTPHVANDYASVCLRTSLQNVGKEEITIETEILDPNGKKVSFKKNSGRINHGQPFTQNFIVENPQLWSPETPFLYQAVSKIYANGKLTDTYTTRFGIRSIEFVADKGFFLNGQHRKFQGVCNHHDLGPLGAAINVSALRHQLTLLKDMGCDAIRTAHNMPAPELVRLCDEMGFMMMIEPFDEWDIAKCENGYHRYFNEWAEKDMVNMLRQYRNNPCVVMWSIGNEVPTQCSSEGYKVAKFLQDICHREDPTRPVTCGMDQVSCVLDNGFAAMLDIPGFNYRAHRYEEAYQRLPQNLVLGSETSSTVSSRGVYKFPAERKADAKYEDHQSSSYDLEYCSWSNIPDIDFALADDHQWTLGQFVWTGFDYLGEPSPYDTDAWPNHSSMFGIIDLASLPKDRYYLYRSIWNKQAETLHILPHWNWEGREGKEVPVFVYTNYPTAELFINGKSYGKQTKNNQSVENRYRLMWHNAIYEPGEVKVVAYDEHGTAKAEKIIRTAGKPHHIELVSSRQSLTADGKDLAYVTVRVVDKDGNLCPTDMRLVKFKVKGAGSYKASANGDPTCLDLFHLPQMHAFNGMLTAIVQSGKEAGTLELQVTAKGLKSGKIQIEVK
CAGCGACATGAACAACTCTTGGAAACCGGCTGGAAATTCCACAAAGGAGAAACCAATGGAGCTGAAACTGTTTCATTTAATGATTCTCAATGGGAATCTGTCTGTATTCCACACGACTGGGCCATTTATGGACCGTTTGACCGTAATAATGATTTACAAAATGTAGCCATTACTCAGAACTTGGAGAAACAGGCATCTGTCAAGACCGGACGTACCGGAGGACTTCCTTATGTGGGAGTAGGATGGTATCGCACCCGTTTCGATGCAGACCCTGACAAAAAGACAACACTGGTTTTTGATGGAGCCATGAGTGAAGCCCGCGTGTATGTCAATGGAAAAGAAGCCTGCTTCTGGCCTTTCGGTTACAATTCCTTCCATTGTGACATTACTGAGCTTCTGCACAAAGAAGGAAAAGACAATGTATTGGCTGTACGTCTGGAAAACCGTCCTCAATCTTCCCGCTGGTATCCGGGAGCCGGACTTTACCGGAATGTCCATCTGATTACTGCAGAAAAAATACATGTACCTGTATGGGGAACACAGGTTACCACCCCACACGTAGCTAATGACTATGCTTCTGTTTGCCTTCGTACCTCTTTACAGAATGTGGGAAAAGAAGAAATTACCATAGAAACAGAAATACTGGACCCGAACGGGAAAAAAGTTTCTTTCAAGAAGAACAGCGGACGCATCAATCACGGGCAACCGTTTACACAAAATTTCATTGTGGAAAACCCGCAATTGTGGTCACCTGAAACACCGTTCTTATATCAGGCCGTATCTAAAATCTATGCCAACGGAAAACTTACAGATACTTATACCACCCGCTTTGGTATCCGTTCCATCGAATTTGTAGCCGACAAGGGCTTTTTCCTGAACGGCCAGCACCGTAAATTCCAGGGGGTATGCAACCACCACGACTTAGGTCCTTTAGGAGCTGCCATCAACGTATCGGCTCTACGCCACCAGCTTACATTATTAAAAGACATGGGCTGCGATGCCATTCGTACCGCACACAACATGCCGGCACCCGAGCTTGTCAGACTCTGCGATGAAATGGGATTCATGATGATGATTGAGCCTTTCGATGAATGGGACATTGCCAAGTGTGAAAACGGATACCACCGCTATTTCAACGAATGGGCCGAAAAAGACATGGTAAACATGCTACGGCAATACCGGAATAATCCCTGTGTGGTGATGTGGAGTATCGGTAATGAAGTACCCACCCAATGCAGCAGTGAAGGATACAAAGTAGCCAAGTTCCTGCAAGACATTTGCCATCGGGAAGACCCTACCCGTCCGGTTACCTGCGGCATGGACCAGGTTAGTTGTGTACTCGACAACGGATTTGCGGCCATGCTCGACATTCCGGGATTCAATTATCGCGCACACCGCTATGAAGAAGCTTACCAACGCCTGCCTCAAAATCTTGTATTAGGCTCAGAAACCTCTTCTACCGTCAGTTCACGCGGTGTATACAAATTCCCGGCAGAGCGTAAAGCCGATGCAAAATACGAAGACCATCAGTCTTCTTCTTACGACTTGGAATACTGCTCCTGGTCTAACATTCCCGATATAGACTTTGCTCTGGCTGATGACCACCAATGGACTTTGGGGCAGTTTGTCTGGACAGGTTTTGATTATCTGGGTGAACCCAGTCCATACGATACGGATGCATGGCCCAACCACAGCTCTATGTTCGGTATTATCGACCTGGCTTCCTTACCCAAAGACCGGTACTATTTATACCGCAGCATATGGAACAAGCAAGCTGAAACACTTCATATTCTTCCTCATTGGAACTGGGAGGGCAGAGAAGGAAAAGAAGTACCTGTATTCGTCTATACCAACTATCCGACAGCCGAACTTTTCATCAACGGAAAAAGTTATGGGAAACAGACGAAGAACAACCAAAGCGTAGAGAACCGTTACCGCCTGATGTGGCACAACGCCATTTACGAACCGGGAGAAGTAAAAGTCGTGGCATACGATGAACACGGTACGGCTAAAGCAGAAAAGATAATCCGCACGGCAGGCAAACCTCACCATATTGAATTGGTTTCTTCACGCCAGTCGCTCACAGCCGATGGAAAAGATTTGGCTTACGTAACCGTACGTGTTGTGGACAAAGACGGAAATCTCTGCCCCACAGATATGCGCTTGGTGAAATTTAAAGTAAAAGGAGCTGGAAGCTACAAAGCCTCAGCCAATGGAGATCCAACTTGTCTGGATTTGTTCCACCTGCCTCAGATGCACGCCTTCAACGGCATGCTGACTGCAATTGTGCAATCAGGAAAAGAAGCAGGTACCCTTGAGTTACAAGTCACCGCAAAAGGGCTGAAATCAGGAAAGATACAAATCGAAGTAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,133 total letters Query= CMR200135.107 Length=2778 Score E Sequences producing significant alignments: (Bits) Value wt 4271 0.0 03E_E3_CMR200135.107_E07.seq 1009 0.0 08E_E3_CMR200135.107_E09.seq 966 0.0 H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N A Query_1 113 ATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCC 202 0 . 0 1076 ........... 1066 0 1076 ........... 1066 2 212 ........... 222 Q R H E Q L L E T G W K F H K G E T N G A E T V S F N D S Q Query_1 203 AGCGACATGAACAACTCTTGGAAACCGGCTGGAAATTCCACAAAGGAGAAACCAATGGAGCTGAAACTGTTTCATTTAATGATTCTCAAT 292 0 2 .......................................................................................... 91 W E S V C I P H D W A I Y G P F D R N N D L Q N V A I T Q N Query_1 293 GGGAATCTGTCTGTATTCCACACGACTGGGCCATTTATGGACCGTTTGACCGTAATAATGATTTACAAAATGTAGCCATTACTCAGAACT 382 0 92 .......................................................................................... 181 L E K Q A S V K T G R T G G L P Y V G V G W Y R T R F D A D Query_1 383 TGGAGAAACAGGCATCTGTCAAGACCGGACGTACCGGAGGACTTCCTTATGTGGGAGTAGGATGGTATCGCACCCGTTTCGATGCAGACC 472 0 182 .......................................................................................... 271 P D K K T T L V F D G A M S E A R V Y V N G K E A C F W P F Query_1 473 CTGACAAAAAGACAACACTGGTTTTTGATGGAGCCATGAGTGAAGCCCGCGTGTATGTCAATGGAAAAGAAGCCTGCTTCTGGCCTTTCG 562 0 272 .......................................................................................... 361 G Y N S F H C D I T E L L H K E G K D N V L A V R L E N R P Query_1 563 GTTACAATTCCTTCCATTGTGACATTACTGAGCTTCTGCACAAAGAAGGAAAAGACAATGTATTGGCTGTACGTCTGGAAAACCGTCCTC 652 0 362 .......................................................................................... 451 0 1815 ..G............ 1829 Q S S R W Y P G A G L Y R N V H L I T A E K I H V P V W G T Query_1 653 AATCTTCCCGCTGGTATCCGGGAGCCGGACTTTACCGGAATGTCCATCTGATTACTGCAGAAAAAATACATGTACCTGTATGGGGAACAC 742 0 452 .......................................................................................... 541 Q V T T P H V A N D Y A S V C L R T S L Q N V G K E E I T I Query_1 743 AGGTTACCACCCCACACGTAGCTAATGACTATGCTTCTGTTTGCCTTCGTACCTCTTTACAGAATGTGGGAAAAGAAGAAATTACCATAG 832 0 542 .......................................................................................... 631 E T E I L D P N G K K V S F K K N S G R I N H G Q P F T Q Query_1 833 AAACAGAAATACTGGACCCGAACGGGAAAA-AAGTTTCTTTCAAGAAGAACAGCGGACGCATCAATC-ACGGGCAACCGTTTACACAAAA 920 0 632 ..............................-....................................-...................... 719 2 650 ..N........NN...N...G...........C...N..........T........NN.........N............ 571 N F I V E N P Q L W S P E T P F L Y Q A V S K I Y A N G K L Query_1 921 TTTCATT-GTGGAAAACCCGCAATTGTGGTCACCTGAAACACCGTTCTTATATCAGGCCGTATCTAAAATCTATGCCAACGGAAAACTTA 1009 0 720 .......-.................................................................................. 808 2 570 .......T....N....N.............N..........................N...........N.N....N.N.......... 481 T D T Y T T R F G I R S I E F V A D K G F F L N G Q H R K F Query_1 1010 CAGAT-ACTTATACCACCCGCTTTGGTATCCGTTCCATCGAATTTGTAGCCGACAAGGGCTTTTTCCTGAACGGCCAGCACCGTAAATTC 1098 0 809 .....-.................................................................................... 897 0 1124 ........... 1114 2 480 .....T..............N...........G....................................N.................... 391 Q G V C N H H D L G P L G A A I N V S A L R H Q L T L L K D Query_1 1099 CAGGGGGTATGCAACCACCACGACTTAGGTCCTTTAGGAGCTGCCATCAACGTATCGGCTCTACGCCACCAGCTTACATTATTAAAAGAC 1188 0 898 .......................................................................................... 987 0 1149 ....... 1155 2 390 ................N...........................................A......N...................... 301 2 139 ....... 133 M G C D A I R T A H N M P A P E L V R L C D E M G F M M M I Query_1 1189 ATGGGCTGCGATGCCATTCGTACCGCACACAACATGCCGGCACCCGAGCTTGTCAGACTCTGCGATGAAATGGGATTCATGATGATGATT 1278 0 988 .......................................................................................... 1077 0 1156 .... 1159 2 300 .......................................................................................... 211 2 132 .... 129 E P F D E W D I A K C E N G Y H R Y F N E W A E K D M V N M Query_1 1279 GAGCCTTTCGATGAATGGGACATTGCCAAGTGTGAAAACGGATACCACCGCTATTTCAACGAATGGGCCGAAAAAGACATGGTAAACATG 1368 0 1078 .......................................................................................... 1167 0 981 ........... 991 2 210 .......................................................................................... 121 2 307 ........... 297 L R Q Y R N N P C V V M W S I G N E V P T Q C S S E G Y Query_1 1369 CTA-CGGCAATA-CCGGAATAAT-CCCTGTGTGGTGATGTGGAGTATCGGTAATGAAGTA-CCCACCCAATGCAGCAGTGAAGGATA-CA 1453 0 1168 ...-........-..........-....................................-..........................-.. 1252 2 120 ...T........N..........A....................................T..........T...............G.N 31 K V A K F L Q D I C H R E D P T R P V T C G M D Q V S C V L Query_1 1454 AAGTAGCCAAGTTCCTGCAAGACATTTGCCATCGGGAAGACCCTACCCGTCCGGTTACCTGCGGCATGGACCAGGTTAGTTGTGTACTCG 1543 0 1253 .......................................................................................... 1342 2 30 ...-N......G...-...... 11 D N G F A A M L D I P G F N A R A H R Y E E A Y Q R L P Query_1 1544 ACAACGGATTTGCGGCCATGCTCGACATTCCGGGATTCAATGC-TCGCG-CACACCG-CTAT-GAAGAAGCTT-ACCAACGCCTGCCTCA 1628 0 1343 .........................................TA-.....-.......-....-..........-................ 1427 1 6 ....N...N..N..T..TC....N.....A.......T..N.T..........T................ 75 Q N L V L G S E T S S T V S S R G V Y K F P A E R K A D A Query_1 1629 AAATCTTG-TATTAGGCTCAGAAACC-TCTTCTACCG-TCAGTTCACGCGGTG-TATACAAATTCCCGGCAGAGCGTAAAGCCGATGCAA 1714 0 1428 ........-.................-..........-...............-.................................... 1513 1 76 ........A.................N..........N.............A.N.................................... 165 K Y E D H Q S S S Y D L E Y C S W S N I P D I D F A L A D D Query_1 1715 AATACGAAGACCATCAGTCTTCTTCTTACGACTTGGAATACTGCTCCTGGTCTAACATTCCCGATATAGACTTTGCTCTGGCTGATGACC 1804 0 1514 .......................................................................................... 1603 1 166 .......................................................N.................................. 255 H Q W T L G Q F V W T G F D Y L G E P S P Y D T D A W P N H Query_1 1805 ACCAATGGACTTTGGGGCAGTTTGTCTGGACAGGTTTTGATTATCTGGGTGAACCCAGTCCATACGATACGGATGCATGGCCCAACCACA 1894 0 1604 .......................................................................................... 1693 1 256 .......................................................................................... 345 S S M F G I I D L A S L P K D R Y Y L Y R S I W N K Q A E T Query_1 1895 GCTCTATGTTCGGTATTATCGACCTGGCTTCCTTACCCAAAGACCGGTACTATTTATACCGCAGCATATGGAACAAGCAAGCTGAAACAC 1984 0 1694 .......................................................................................... 1783 1 346 .......................................................................................... 435 L H I L P H W N W E G R E G K E V P V F V Y T N Y P T A E Query_1 1985 TTCATATTCTTCCTCATTGGAACTGGGAGGGCAGAGAAGGAAAAGAAGTACCTGTATTCG-TCTATACCAACTATC-CGACAGCCGAACT 2072 0 1784 ............................................................-...............-............. 1871 0 402 ..A............ 416 1 436 ...................................N........................N...............N..........T.. 525 L F I N G K S Y G K Q T K N N Q S V E N R Y R L M W H N A I Query_1 2073 TTTCATCAACGGAAAAAGTTATGGGAAACAGACGAAGAACAACCAAAGCGTAGAGAACCGTTACCGCCTGATGTGGCACAACGCCA-TTT 2161 0 1872 ......................................................................................-... 1960 1 526 ...................................N........NN......C..................N.N......N....NT... 615 Y E P G E V K V V A Y D E H G T A K A E K I I R T A G K Query_1 2162 AC-GAACCGGGAGAAGTAAAAGTCGTGGCATACGATGAACACGGTACGGCTAAAG-CAGAAAAGATAATCC-GCA-CGGCA--GGCAAAC 2245 0 1961 ..-....................................................-...............-...-.....--....... 2044 1 616 ..TC....N......N........NN......TN......N........T.....N..........N....N...C.....AG.TN..NN 705 P H H I E L V S S R Q S L T A D G K D L A Y V T V R V V D Query_1 2246 CT-CACCAT-ATTG-AATTGGTTT-CTTCACGCCAGTCGCTCACAGCCGATGGAAAAGATTTGGCTTACGTAACCGTACGTGTTGTGGAC 2331 0 2045 ..-......-....-.........-................................................................. 2130 1 706 ..C.N....N....A....N....N...... 736 K D G N L C P T D M R L V K F K V K G A G S Y K A S A N G D Query_1 2332 AAAGACGGAAATCTCTGCCCCACAGATATGCGCTTGGTGAAATTTAAAGTAAAAGGAGCTGGAAGCTACAAAGCCTCAGCCAATGGAGAT 2421 0 2131 .......................................................................................... 2220 P T C L D L F H L P Q M H A F N G M L T A I V Q S G K E A G Query_1 2422 CCAACTTGTCTGGATTTGTTCCACCTGCCTCAGATGCACGCCTTCAACGGCATGCTGACTGCAATTGTGCAATCAGGAAAAGAAGCAGGT 2511 0 2221 .......................................................................................... 2310 0 334 ........... 344 0 1821 ........... 1831 1 473 ........... 483 T L E L Q V T A K G L K S G K I Q I E V K Query_1 2512 ACCCTTGAGTTACAAGTCACCGCAAAAGGGCTGAAATCAGGAAAGATACAAATCGAAGTAAAA 2574 0 2311 ............................................................... 2373 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 14031402 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:02 AM Number of letters in database: 5,133 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200148.108 (CMR200148.28-761.P(761).D289A.pMCSG68)
KDAEMDRFISDLMGRMTLQEKLGQLNLPAGNDLVSGAVKNSKMAEAIRAGEVGGFFNVKGVDKIYQMQRMAVEETRLGIPLIVGADVIHGYETIFPIPLALSCSWDTAAVTRMARISATEASADGISWTFSPMVDICRDARWGRIAEGSGEDPYLGALMAGAYVRGYQGDGMKQNNEIMACVKHFALYGASESGRDYNSVDMSRNLMYNVYLAPYKGAVEAGVGSVMSSFNTINGVPATADKWLLTDLLRNEWGFTGFVVTDYNSIGEMKTHGVADLKEASARALNAGTDMDMVAHGFLHTLEASLKEKAVTQERIDEACRRVLEAKYKLGLFENPYKYCDTLRGRKELFTEANRKAAREIAAETFVLLKNEGKLLPLQKKGRIALIGPMADAQNNMCGTWNMDCQTDRHVTMYEAFRRAVGDKATVSYAKGSNVYYSEHIEKGAVEPRPLTRGDDRQLRAEALRVAASADVIVAALGESAEMSGESSSRTDIQIPDAQKDLLKALIATGKPVVLALFTGRPLDLCWESEHVPAILNVWFAGSEAGDAIADVMFGEVSPSGKLTTSFPRAVGQLPLYYNHLNTGRPDTDDTTFNRYGSNYIDQSNEPLYPFGYGLSYTTFRYGNLQLSAERMAKGGQLKVTVPVTNSGECDGVEIVQLYLHDVYAEISRPVKELKAFRRVALKKGETQNVEFVLDEDDLKYYNSRLEYGYEPGEFEVMVGPDSRNVQHATFVAE
AAGGATGCGGAGATGGACCGCTTTATCAGTGACCTGATGGGAAGGATGACCTTGCAGGAAAAGTTAGGACAGTTGAATCTGCCGGCTGGGAATGACCTGGTGTCGGGAGCAGTGAAGAACAGCAAGATGGCAGAAGCTATCCGAGCTGGTGAGGTCGGCGGCTTTTTCAATGTGAAGGGAGTGGATAAGATTTACCAGATGCAGCGTATGGCGGTGGAGGAAACTCGTCTGGGAATTCCTTTGATAGTGGGTGCCGATGTGATTCACGGGTACGAAACAATCTTCCCGATTCCGTTGGCCCTGTCTTGTAGCTGGGATACGGCGGCGGTGACACGTATGGCACGTATTTCTGCCACGGAAGCCAGTGCCGATGGAATCAGCTGGACCTTCAGTCCGATGGTAGACATCTGTCGGGATGCCCGCTGGGGACGTATTGCAGAAGGAAGTGGAGAGGACCCGTACCTCGGGGCGTTGATGGCTGGAGCCTATGTGCGCGGTTATCAGGGTGACGGCATGAAGCAGAACAATGAAATCATGGCCTGTGTGAAGCACTTTGCGCTGTATGGAGCTTCGGAATCGGGACGTGACTACAATTCGGTGGATATGAGTCGAAACCTGATGTATAATGTGTACCTGGCTCCTTATAAAGGGGCGGTGGAAGCCGGAGTGGGTTCGGTGATGAGCTCGTTCAATACCATCAACGGGGTACCTGCTACAGCTGACAAATGGCTGCTGACGGATTTGCTCCGCAATGAGTGGGGGTTCACGGGGTTTGTGGTGACCGACTACAATTCGATTGGTGAGATGAAGACTCATGGGGTGGCCGACTTGAAGGAGGCTTCTGCACGGGCGTTGAATGCAGGAACGGACATGGATATGGTGGCACATGGTTTCTTGCATACGCTGGAAGCTTCATTGAAGGAGAAGGCCGTGACGCAGGAGCGGATTGACGAGGCTTGTCGTCGGGTATTGGAAGCCAAGTATAAGTTAGGATTGTTTGAAAATCCTTATAAGTATTGTGATACGCTTCGGGGACGCAAGGAATTGTTTACGGAGGCGAATCGTAAAGCGGCACGTGAGATTGCGGCTGAAACGTTTGTGCTGTTGAAGAACGAGGGTAAGTTGTTGCCTTTGCAGAAAAAAGGACGCATTGCATTGATTGGGCCGATGGCTGATGCGCAGAACAATATGTGCGGCACGTGGAACATGGATTGTCAGACAGACCGTCATGTGACGATGTACGAAGCTTTCCGTCGTGCGGTAGGTGATAAGGCTACGGTTTCTTATGCCAAGGGAAGTAATGTGTATTATAGTGAGCATATTGAGAAAGGGGCGGTCGAACCTCGTCCGCTGACACGTGGCGATGACCGTCAGTTGCGGGCTGAGGCTTTGCGCGTGGCGGCTTCTGCCGATGTGATTGTGGCCGCATTAGGTGAGAGTGCTGAGATGAGCGGAGAGTCTTCTTCTCGTACAGATATTCAGATTCCGGATGCGCAGAAAGATTTGTTGAAGGCATTGATAGCTACCGGAAAGCCGGTGGTACTGGCTTTGTTTACCGGTCGTCCGCTGGATTTATGCTGGGAGTCTGAGCATGTTCCGGCTATCCTGAACGTGTGGTTTGCCGGCAGTGAAGCGGGTGATGCCATTGCCGATGTGATGTTTGGAGAAGTATCTCCTTCGGGTAAGCTGACTACGAGTTTCCCACGTGCGGTGGGACAGTTGCCGCTTTATTATAATCACCTGAATACGGGTCGTCCGGATACGGATGACACTACTTTCAATCGTTATGGCAGCAATTACATCGACCAGAGTAATGAACCGCTTTATCCTTTTGGCTATGGTTTGAGTTATACCACTTTCCGTTACGGTAATTTGCAGTTGAGTGCGGAGCGTATGGCCAAGGGTGGGCAGTTGAAGGTAACCGTGCCTGTAACCAATTCCGGCGAGTGTGACGGAGTAGAGATTGTGCAGTTGTATCTTCACGATGTGTATGCAGAAATCTCCCGTCCGGTGAAGGAGCTGAAAGCTTTCCGCCGTGTGGCCCTTAAAAAGGGAGAGACACAGAATGTAGAGTTTGTACTCGATGAGGATGATTTGAAGTATTATAATTCTCGTCTGGAATATGGATATGAACCGGGAGAGTTTGAAGTGATGGTGGGTCCGGACAGCCGGAATGTGCAGCACGCGACTTTTGTGGCTGAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,935 total letters Query= CMR200148.108 Length=2607 Score E Sequences producing significant alignments: (Bits) Value wt 3963 0.0 08F_F3_CMR200148.108_F09.seq 1308 0.0 03F_F3_CMR200148.108_F07.seq 1195 0.0 K D A E M D R F I S D L M G R M T L Q E K L G Q L N L P A G Query_1 202 AAGGATGCGGAGATGGACCGCTTTATCAGTGACCTGATGGGAAGGATGACCTTGCAGGAAAAGTTAGGACAGTTGAATCTGCCGGCTGGG 291 0 1 .......................................................................................... 90 0 1814 ...A........... 1828 N D L V S G A V K N S K M A E A I R A G E V G G F F N V K G Query_1 292 AATGACCTGGTGTCGGGAGCAGTGAAGAACAGCAAGATGGCAGAAGCTATCCGAGCTGGTGAGGTCGGCGGCTTTTTCAATGTGAAGGGA 381 0 91 .......................................................................................... 180 V D K I Y Q M Q R M A V E E T R L G I P L I V G A D V I H G Query_1 382 GTGGATAAGATTTACCAGATGCAGCGTATGGCGGTGGAGGAAACTCGTCTGGGAATTCCTTTGATAGTGGGTGCCGATGTGATTCACGGG 471 0 181 .......................................................................................... 270 0 1407 ............. 1419 2 162 ............. 150 Y E T I F P I P L A L S C S W D T A A V T R M A R I S A T E Query_1 472 TACGAAACAATCTTCCCGATTCCGTTGGCCCTGTCTTGTAGCTGGGATACGGCGGCGGTGACACGTATGGCACGTATTTCTGCCACGGAA 561 0 271 .......................................................................................... 360 0 582 ........... 572 A S A D G I S W T F S P M V D I C R D A R W G R I A E G S G Query_1 562 GCCAGTGCCGATGGAATCAGCTGGACCTTCAGTCCGATGGTAGACATCTGTCGGGATGCCCGCTGGGGACGTATTGCAGAAGGAAGTGGA 651 0 361 .......................................................................................... 450 E D P Y L G A L M A G A Y V R G Y Q G D G M K Q N N E I M A Query_1 652 GAGGACCCGTACCTCGGGGCGTTGATGGCTGGAGCCTATGTGCGCGGTTATCAGGGTGACGGCATGAAGCAGAACAATGAAATCATGGCC 741 0 451 .......................................................................................... 540 C V K H F A L Y G A S E S G R D Y N S V D M S R N L M Y N V Query_1 742 TGTGTGAAGCACTTTGCGCTGTATGGAGCTTCGGAATCGGGACGTGACTACAATTCGGTGGATATGAGTCGAAACCTGATGTATAATGTG 831 0 541 .......................................................................................... 630 0 784 ............ 795 Y L A P Y K G A V E A G V G S V M S S F N T I N G V P A T Query_1 832 TACCTGGCTCCTTATAAAGGGGCGGTGGAAGCCGGAGTGGGTTCGGTGATGAGCTCGTTCAATACCATCAACGGGGTACCT-GCT-ACAG 919 0 631 .................................................................................-...-.... 718 0 1327 ...........C... 1341 2 889 .........N.N.N.... 872 2 242 ...........C... 228 1 21 ..N..............-...-.... 44 A D K W L L T D L L R N E W G F T G F V V T A Y N Query_1 920 C-TGA--CAAATGGCTG-C-TGA-CGGA--TTTGCT-CCGCAATGAGTGGGG-GTTCACGGGG--TTTGTGGTGACC-GCTTAC--AATT 994 0 719 .-...--..........-.-...-....--......-...............-..........--............-.AC...--.... 793 2 871 .C...NN..NN......G.-.NNN....AT..N...T..N...NN..NN...N..........NN..N.........C.N..N.CA.N.C 783 1 45 .-...--..........-.N...-....--......-...............-..........--............-......--.... 120 S I G E M K T H G V A D L K E A S A R A L N A G T D M D Query_1 995 CGATTGGTGAGATGAAGACT-CATGGGGT--GGCCG-ACTTGAAGG-AGGCTTCTGCACGGGCGTTGAATGCAGGAACGGACATGGATAT 1079 0 794 ....................-........--.....-.........-........................................... 878 2 782 .......N....N...N..NN.N....N.TNN....N.........N.......N...............N.....N............. 693 1 121 ....................-........--.....-.........-........................................... 205 M V A H G F L H T L E A S L K E K A V T Q E R I D E A C R R Query_1 1080 GGTGGCACATGGTT-TCTTGCATACGCTGGAAGCTTCATTGAAGGAGAAGGCCGTGACGCAGGAGCGGATTGACGAGGCTTGTCGTCGGG 1168 0 879 ..............-........................................................................... 967 2 692 ..............C........................................................................... 603 1 206 ..............-........................................................................... 294 V L E A K Y K L G L F E N P Y K Y C D T L R G R K E L F T E Query_1 1169 TATTGGAAGCCAAGTATAAGTTAGGATTGTTTGAAAATCCTTATAAGTATTGTGATACGCTTCGGGGACGCAAGGAATTGTTTACGGAGG 1258 0 968 .......................................................................................... 1057 2 602 .....................................................N.................................... 513 1 295 .......................................................................................... 384 A N R K A A R E I A A E T F V L L K N E G K L L P L Q K K G Query_1 1259 CGAATCGTAAAGCGGCACGTGAGATTGCGGCTGAAACGTTTGTGCTGTTGAAGAACGAGGGT-AAGTTGTTGCCTTTGCAGAAAAAAGGA 1347 0 1058 ..............................................................-........................... 1146 2 512 ..............................................................C........................... 423 1 385 ..............................................................-........................... 473 R I A L I G P M A D A Q N N M C G T W N M D C Q T D R H V T Query_1 1348 CGCATTGCATTGATTGGGCCGATGGCTGATGCGCAGAACAATATGTGCGGCACGTGGAACATGGATTGTCAGACAGACCGTCATGTGACG 1437 0 1147 .......................................................................................... 1236 0 1489 ...........AG..T... 1507 2 422 .................N........................................................................ 333 2 80 ...........AG..T... 62 1 474 ................................N......................................N.................. 563 M Y E A F R R A V G D K A T V S Y A K G S N V Y Y S E H I E Query_1 1438 ATGTACGAAGCTTTCCGTCGTGCGGTAGGTGATAAGGCTACGGTTTCTTATGCCAAGGGAAGTAATGTGTATTATAGTGAGCATATTGAG 1527 0 1237 .......................................................................................... 1326 2 332 .......................................................................................... 243 1 564 ...................NN....N.........N....................................N...N...N......... 653 K G A V E P R P L T R G D D R Q L R A E A L R V A A S A D Query_1 1528 AAAGGGGCGGTCGAACC-TCGTCCGCTGACACGTGGCGATGACCGTCAGTTGCGGGC-TGAGGCTTTGCGCGTGGCGGCTTCTG-CCGAT 1614 0 1327 .................-.......................................-..........................-..... 1413 0 646 ...........G... 660 0 252 ..-..... 258 2 242 .................-.......................................-..........................-..... 156 1 654 .....T..N...N....T..NN.............N..........N.N....NNN.T.N.........N..............C..... 743 V I V A A L G E S A E M S G E S S S R T D I Q I P D A Q K D Query_1 1615 GTGATTGTGGCCGCATTAGGTGAGAGTGCTGAGATGAGCGGAGAGTCTTCTTCTCGTACAGATATTCAGATTCCGGATGCGCAGAAAGAT 1704 0 1414 .......................................................................................... 1503 0 1174 ...........CA.. 1188 0 259 ...... 264 2 155 .......................................................................................... 66 2 395 ...........CA.. 381 1 744 N..... 749 L L K A L I A T G K P V V L A L F T G R P L D L C W E S E Query_1 1705 TTGTTGAAGGCATTGATAGCTACCGG-AAAGCCGGTGGTACTGGCTTTGTTTACCGGTCGTCCGCTGGATTTATGCTGGGAGTCTGAGCA 1793 0 1504 ..........................-............................................................... 1592 0 1189 A... 1192 2 65 ..........................A........................A...C.. 8 2 380 A... 377 H V P A I L N V W F A G S E A G D A I A D V M F G E V S P S Query_1 1794 TGTTCCGGCTATCCTGAACGTGTGGTTTGCCGGCAGTGAAGCGGGTGATGCCATTGCCGATGTGATGTTTGGAGAAGTATCTCCTTCGGG 1883 0 1593 .......................................................................................... 1682 0 252 ............ 263 G K L T T S F P R A V G Q L P L Y Y N H L N T G R P D T D D Query_1 1884 TAAGCTGACTACGAGTTTCCCACGTGCGGTGGGACAGTTGCCGCTTTATTATAATCACCTGAATACGGGTCGTCCGGATACGGATGACAC 1973 0 1683 .......................................................................................... 1772 T T F N R Y G S N Y I D Q S N E P L Y P F G Y G L S Y T T F Query_1 1974 TACTTTCAATCGTTATGGCAGCAATTACATCGACCAGAGTAATGAACCGCTTTATCCTTTTGGCTATGGTTTGAGTTATACCACTTTCCG 2063 0 1773 .......................................................................................... 1862 0 13 ...G........... 27 R Y G N L Q L S A E R M A K G G Q L K V T V P V T N S G E C Query_1 2064 TTACGGTAATTTGCAGTTGAGTGCGGAGCGTATGGCCAAGGGTGGGCAGTTGAAGGTAACCGTGCCTGTAACCAATTCCGGCGAGTGTGA 2153 0 1863 .......................................................................................... 1952 D G V E I V Q L Y L H D V Y A E I S R P V K E L K A F R R V Query_1 2154 CGGAGTAGAGATTGTGCAGTTGTATCTTCACGATGTGTATGCAGAAATCTCCCGTCCGGTGAAGGAGCTGAAAGCTTTCCGCCGTGTGGC 2243 0 1953 .......................................................................................... 2042 A L K K G E T Q N V E F V L D E D D L K Y Y N S R L E Y G Y Query_1 2244 CCTTAAAAAGGGAGAGACACAGAATGTAGAGTTTGTACTCGATGAGGATGATTTGAAGTATTATAATTCTCGTCTGGAATATGGATATGA 2333 0 2043 .......................................................................................... 2132 E P G E F E V M V G P D S R N V Q H A T F V A E Query_1 2334 ACCGGGAGAGTTTGAAGTGATGGTGGGTCCGGACAGCCGGAATGTGCAGCACGCGACTTTTGTGGCTGAA 2403 0 2133 ...................................................................... 2202 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12649560 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:02 AM Number of letters in database: 4,935 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2