Clone ID: APC113179.120 (APC113179.3-243.P(247).S191P.pMCSG68)
VDQYKKEIGMMNLEKLIQDREGISSKKFPIEDKELLFRYEQLYTGAVNDVMREFCLLEQALPGRIKPLREYHSVAGFAFTVKSAPNVKIKGEMEYRTQMLDEMQEDHFVVWDTSRDDKATLWGGVMTATAKGKKLKAACIDGGIRDTHQILNADFPVFYEYRISNGSLGRCLITHYQIPIKIGDVTIKPGDIILGDIDGVLVVPREIAYEVLLRSEEIRENEKKIFSWVQNGDSVQEITAK
GTAGATCAGTATAAGAAAGAAATCGGAATGATGAATCTAGAAAAATTGATTCAGGATCGTGAAGGAATATCTTCGAAAAAGTTTCCTATAGAGGATAAAGAATTATTATTTCGATACGAGCAGTTGTATACAGGGGCCGTAAACGATGTGATGCGTGAATTTTGCCTTTTGGAGCAAGCCTTACCCGGTAGGATTAAACCTTTACGCGAATATCATTCTGTTGCCGGTTTTGCCTTTACTGTCAAAAGTGCACCAAACGTAAAGATAAAGGGTGAGATGGAATATCGAACCCAGATGCTGGATGAGATGCAGGAAGACCATTTTGTTGTCTGGGATACAAGTCGGGATGATAAAGCTACTTTGTGGGGAGGTGTAATGACGGCAACTGCTAAAGGGAAGAAACTGAAAGCCGCTTGTATAGATGGGGGAATACGGGATACACATCAGATTTTGAATGCTGATTTTCCTGTGTTTTACGAATATCGTATATCGAATGGTAGCTTGGGGCGCTGTTTGATTACTCACTATCAGATTCCAATTAAAATCGGAGATGTAACGATTAAGCCGGGTGATATAATACTTGGAGATATCGATGGAGTTTTAGTTGTACCAAGGGAAATAGCTTATGAAGTGCTTTTACGCTCGGAGGAGATACGTGAAAATGAGAAGAAGATTTTCTCTTGGGTACAAAACGGAGACAGTGTTCAGGAAATTACAGCAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,409 total letters Query= APC113179.120 Length=1128 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1305 0.0 2_08G_G3_APC113179.120T7term.seq 1182 0.0 1_03G_G3_APC113179.120T7sh.seq 1182 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 59 TCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATT-CTTCTGGTGTAGATCTGTGG 147 1 4 .......-....-........N.......................................C.C.TT..N.NN..............N.. 91 S H P Q F E K G T E N L Y F Q S N A V D Q Y K K E I G M M N Query_1 148 TCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGTAGATCAGTATAAGAAAGAAATCGGAATGATGAAT 237 0 1 .................................... 36 1 92 ................CNTT...........................................N.......................... 181 L E K L I Q D R E G I S S K K F P I E D K E L L F Query_1 238 CTAGAAAAATTGA--TTCAGG-ATC-GTG-AAGGAA-TATCTTCGAAAAA--GTTT-CCT-ATAGAGG--ATAAA-GAATTATTATT-TC 313 0 37 .............--......-...-...-......-.............--....-...-.......--.....-...........-.. 112 2 826 .........AT......T...N...G......A.......NN....AT....N...N....GN.GN.N...N...........A.. 741 1 182 .............--......-...-...-......-.............--....-...-.......--.....-.......N...-.. 257 R Y E Q L Y T G A V N D V M R E F C L L E Q A L P G R I Query_1 314 GATA-CG-AGCA-GTTGTATACAGGGG-CCGTAAA-CGATGTGATGCGTGAATTTTGCCTTTTGGAGCAAGCCTTACCCGGTAGGATTAA 398 0 113 ....-..-....-..............-.......-...................................................... 197 2 740 ...NN..N...NN.....N........N.......A.....N............N.............N...NN.....N.A........ 651 1 258 ....-..-....-..............-.......-............N............N............................ 342 K P L R E Y H S V A G F A F T V K S A P N V K I K G E M E Y Query_1 399 ACCTTTACGCGAATATCATTC-TGTTGCCGGTTTTGCCTTTACTGTCAAAAGTGCACCAAACGTAAAGATAAAGGGTGAGA-TGGAATAT 486 0 198 .....................-...........................................................-........ 285 2 650 ....N.N..............C.........N..A.....NCN......................................A......N. 561 1 343 .....................-......................................................N....-........ 430 R T Q M L D E M Q E D H F V V W D T S R D D K A T L W G G V Query_1 487 CGAACCCAGATGCTGGATGAGATGCAGGAAGACCATTTTGTTGTCTGGGATACAAGTCGGGATGATAAAGCTACTTTGTGGGGAGGTGTA 576 0 286 .......................................................................................... 375 2 560 ...N...N.N..N..............N.............................................................. 471 1 431 ......................................N...........N...........................C........... 520 M T A T A K G K K L K A A C I D G G I R D T H Q I L N A D Query_1 577 ATGACGGCAACTGCTAAAGGGAAGAAACTGAAAGCCGCTTGTATAGATGGGGGAATACGGGATACACATCAGATTTTGAATGCTGATTT- 665 0 376 .........................................................................................- 464 2 470 .........................................................................................- 382 1 521 .........................................N..........................C.N..................C 610 F P V F Y E Y R I S N G S L G R C L I T H Y Q I P I K I G Query_1 666 TCCTGTGTTTTACGAATAT-CGTATATCGAATGGTAGCTT-GGGGCGCTGTTTGATTACTCACTATCAG-ATTCCAATTAAAATCGGAGA 752 0 465 ...................-....................-............................-.................... 551 2 381 ...................-....................-............................-.................... 295 1 611 .......N...........C....................G.........N..................N.N.....N.N.......... 700 D V T I K P G D I I L G D I D G V L V V P R E I A Y E Query_1 753 TGTAACGATTAAG-CCGGGTGATATAATA-CTTGGAG-ATATCGAT--GGAGTTTTAGTT-GTACCA-AGGG-AAATAGCTT-ATGAAGT 833 0 552 .............-...............-.......-........--............-......-....-.........-....... 632 2 294 .............-...............-.......-........--............-..N...-....-.........-....... 214 1 701 .............C.....N..C.N....C.......C...N.NGNCG....N..C....G.C....C....A...N.....A.N..... 790 V L L R S E E I R E N E K K I F S W V Q N G D S V Q Query_1 834 GCTTTTA-CGCTCGG--AGGAGATACGT-GAAAATGA-GAAGAAG-ATTTTCTCTT--GGGTACAA-AACGGA-GACAGTGTTCA--GGA 911 0 633 .......-.......--...........-........-.......-..........--........-......-...........--... 710 2 213 .......-.......--...........-........-.......-..........--........-......-...........--... 136 1 791 ......NC.C...N.NA....C.N....N.....N..CN......A.C........TG...N....N......NN..G...N...AG... 880 E I T A K * H W K W I T D P N S S S V D K L A A A L E H H H Query_1 912 AA-TTACAGCAAAATAACATTGGAAGTGGATAA-CGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccac 999 0 711 ..-........... 723 2 135 ..-..............................T................................................N....... 47 1 881 ..T....GC..... 894 H H H * D P A A N K A R K E Query_1 1000 cacca-ccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAG 1042 2 46 ....NC............N.......T........N..N..... 3 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3737432 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:35 AM Number of letters in database: 3,409 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC113179.121 (APC113179.3-247.P(247).S191P.pMCSG68)
VDQYKKEIGMMNLEKLIQDREGISSKKFPIEDKELLFRYEQLYTGAVNDVMREFCLLEQALPGRIKPLREYHSVAGFAFTVKSAPNVKIKGEMEYRTQMLDEMQEDHFVVWDTSRDDKATLWGGVMTATAKGKKLKAACIDGGIRDTHQILNADFPVFYEYRISNGSLGRCLITHYQIPIKIGDVTIKPGDIILGDIDGVLVVPREIAYEVLLRSEEIRENEKKIFSWVQNGDSVQEITAKGGYF
GTAGATCAGTATAAGAAAGAAATCGGAATGATGAATCTAGAAAAATTGATTCAGGATCGTGAAGGAATATCTTCGAAAAAGTTTCCTATAGAGGATAAAGAATTATTATTTCGATACGAGCAGTTGTATACAGGGGCCGTAAACGATGTGATGCGTGAATTTTGCCTTTTGGAGCAAGCCTTACCCGGTAGGATTAAACCTTTACGCGAATATCATTCTGTTGCCGGTTTTGCCTTTACTGTCAAAAGTGCACCAAACGTAAAGATAAAGGGTGAGATGGAATATCGAACCCAGATGCTGGATGAGATGCAGGAAGACCATTTTGTTGTCTGGGATACAAGTCGGGATGATAAAGCTACTTTGTGGGGAGGTGTAATGACGGCAACTGCTAAAGGGAAGAAACTGAAAGCCGCTTGTATAGATGGGGGAATACGGGATACACATCAGATTTTGAATGCTGATTTTCCTGTGTTTTACGAATATCGTATATCGAATGGTAGCTTGGGGCGCTGTTTGATTACTCACTATCAGATTCCAATTAAAATCGGAGATGTAACGATTAAGCCGGGTGATATAATACTTGGAGATATCGATGGAGTTTTAGTTGTACCAAGGGAAATAGCTTATGAAGTGCTTTTACGCTCGGAGGAGATACGTGAAAATGAGAAGAAGATTTTCTCTTGGGTACAAAACGGAGACAGTGTTCAGGAAATTACAGCAAAAGGTGGTTACTTT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,129 total letters Query= APC113179.121 Length=1140 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1326 0.0 1_03H_H3_APC113179.121T7sh.seq 230 2e-63 2_08H_H3_APC113179.121T7term.seq 212 4e-58 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 57 ATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTG 146 1 4 ...T....TCN....N.-.................................................N...................... 92 W S H P Q F E K G T E N L Y F Q S N A V D Q Y K K E I G M M Query_1 147 GTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGTAGATCAGTATAAGAAAGAAATCGGAATGATGAA 236 0 1 ................................... 35 1 93 ....................................................... 147 N L E K L I Q D R E G I S S K K F P I E D K E L L F R Y E Q Query_1 237 TCTAGAAAAATTGATTCAGGATCGTGAAGGAATATCTTCGAAAAAGTTTCCTATAGAGGATAAAGAATTATTATTTCGATACGAGCAGTT 326 0 36 .......................................................................................... 125 L Y T G A V N D V M R E F C L L E Q A L P G R I K P L R E Y Query_1 327 GTATACAGGGGCCGTAAACGATGTGATGCGTGAATTTTGCCTTTTGGAGCAAGCCTTACCCGGTAGGATTAAACCTTTACGCGAATATCA 416 0 126 .......................................................................................... 215 H S V A G F A F T V K S A P N V K I K G E M E Y R T Q M L D Query_1 417 TTCTGTTGCCGGTTTTG--CCTTTACTGTCAAAAGTGCACCAAACGTAAAGATAAAGGGTGAGATGGAATATCGAACCCAGATGCTGGAT 504 0 216 .................--....................................................................... 303 2 523 ...........AT...... 505 E M Q E D H F V V W D T S R D D K A T L W G G V M T A T A K Query_1 505 GAGATGCAGGAAGACCATTTTGTTGTCTGGGATACAAGTCGGGATGATAAAGCTACTTTGTGGGGAGGTGTAATGACGGCAACTGCTAAA 594 0 304 .......................................................................................... 393 G K K L K A A C I D G G I R D T H Q I L N A D F P V F Y E Y Query_1 595 GGGAAGAAACTGAAAGCCGCTTGTATAGATGGGGGAATACGGGATACACATCAGATTTTGAATGCTGATTTTCCTGTGTTTTACGAATAT 684 0 394 .......................................................................................... 483 1 460 ........... 450 R I S N G S L G R C L I T H Y Q I P I K I G D V T I K P G D Query_1 685 CGTATATCGAATGGTAGCTTGGGGCGCTGTTTGATTACTCACTATCAGATTCCAATTAAAATCGGAGATGTAACGATTAAGCCGGGTGAT 774 0 484 .......................................................................................... 573 I I L G D I D G V L V V P R E I A Y E V L L R S E E I R E N Query_1 775 ATAATACTTGGAGATATCGATGGAGTTTTAGTTGTACCAAGGGAAATAGCTTATGAAGTGCTTTTACGCTCGGAGGAGATACGTGAAAAT 864 0 574 .......................................................................................... 663 E K K I F S W V Q N G D S V Q E I T A K G G Y F * H W K W I Query_1 865 GAGAAGAAGATTTTCTCTTGGGTACAAAACGGAGACAGTGTTCAGGAAATTACAGCAAAAGGTGGTTACTTTTAACATTGGAAGTGGATA 954 0 664 ........................................................................ 735 1 234 ........... 224 2 124 ................... 106 T D P N S S S V D K L A A A L E H H H H H H * D P A A N K A Query_1 955 ACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCC 1044 2 105 .......................................................................................... 16 R K E Query_1 1045 CGAAAGGAAGC 1055 2 15 .....A..... 5 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3463044 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:35 AM Number of letters in database: 3,129 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC114117.102 (APC114117.1-254.F..pMCSG68)
MTREFIMLMVPPLTDGFPLDILVANVVACFLLGTVTALYARKIHSRDVHTIIGTGMMGGVSTFSSFAYGSVVLASASMSAFLIAAAYVTVSVVAGYVAVLAGMKFGEKSADILHRYPPMASIIDSGLVTVESRHSVAETIERVAAKAKSMGMNVFTRVDHGAGAKEAGLGLPPTELIIFGNPQNGTVLMQDKRTIGLDLPIRALAWEDGSGKVWLTVNDPAWLAQRHSLGLSSDVAIKAMVTGTGTVTKYAAGD
ATGACGCGCGAATTCATCATGCTCATGGTGCCGCCGCTGACGGACGGTTTTCCGCTCGATATTCTTGTGGCCAATGTGGTCGCCTGTTTTCTCCTCGGTACGGTGACTGCGCTTTATGCCAGGAAGATTCATTCCCGCGATGTGCACACCATAATCGGCACGGGCATGATGGGCGGGGTCTCCACCTTTTCCAGCTTTGCCTATGGCTCGGTCGTGCTGGCCTCCGCTTCGATGTCGGCTTTTCTCATCGCTGCCGCCTATGTCACGGTGAGCGTGGTTGCTGGTTATGTCGCGGTTCTGGCCGGAATGAAATTCGGTGAGAAAAGTGCAGATATTCTGCACCGATATCCCCCGATGGCGAGCATTATCGATAGCGGCCTTGTAACGGTCGAGAGCCGCCACTCCGTCGCTGAAACCATCGAACGTGTCGCGGCGAAGGCGAAGTCGATGGGGATGAATGTTTTCACCCGTGTCGATCACGGGGCCGGAGCCAAAGAGGCGGGGCTGGGCTTGCCGCCGACCGAACTCATCATTTTCGGAAACCCGCAAAACGGCACGGTTTTGATGCAGGACAAGAGAACCATCGGCCTTGATCTTCCCATTCGCGCACTTGCCTGGGAAGACGGCAGCGGCAAGGTCTGGCTCACGGTCAATGATCCGGCCTGGCTCGCACAACGCCACAGCCTGGGTTTGTCGAGTGACGTGGCTATCAAGGCGATGGTGACTGGAACCGGCACCGTCACGAAATATGCCGCCGGGGAT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 9 sequences; 10,142 total letters Query= APC114117.102 Length=1167 Score E Sequences producing significant alignments: (Bits) Value 6_06B_B1_APC114117.102BT7term.seq 1797 0.0 5_06A_A1_APC114117.102AT7term.seq 1705 0.0 2_01B_B1_APC114117.102BT7sh.seq 1680 0.0 4_01F_F1_APC114117.102DT7sh.seq 1496 0.0 3_01E_E1_APC114117.102CT7sh.seq 1490 0.0 8_06F_F1_APC114117.102DT7term.seq 1460 0.0 7_06E_E1_APC114117.102CT7term.seq 1460 0.0 1_01A_A1_APC114117.102AT7sh.seq 1402 0.0 0_wt 1375 0.0 S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 5 GATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAAC-AATTCCCCTCTA-GAAATAATTTTGTTTAACTTTAAGA 92 6 1090 .....N................M........A...GNNTGNN.N.....N..........NNN......................... 1003 5 1070 ...T...N.....--..T........-......-.....N.....-.....-.......-...N--.........-.............. 991 2 7 .........N-...-..................... 40 4 14 ..............-........ 35 3 3 .-.......-.G..-...-..................... 38 8 981 ...........................................-.........G..-......................... 902 8 926 ...C...........T.T.C.C.C..-.........A..-. 964 7 979 ...........................................-.........G..-......................... 900 7 924 ...C...........T.T.C.C.C..-.........A..-. 962 1 17 ................... 35 E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E Query_1 93 AGGAGATATAC-ATATGCACC-ATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCA-TCCGCAGTTCGAAAA-GGGTACCGA-G 177 6 1002 ...........-.........-......................KCN.......N.N....N-.........N.....A.........-. 917 5 990 ..A........N.......N.N.................NN-..N...N.....N.-.....A..........N....-.........A. 904 2 41 ...........-.........-........................................-...............-.........-. 125 4 52 ..-. 54 4 36 ...........-..... 51 3 55 ..-. 57 3 39 ...........-..... 54 8 885 ..-. 883 8 901 ...........-..... 886 7 883 ..-. 881 7 899 ...........-..... 884 1 36 ...........-.........-........................................-...............-.........-. 120 N L Y F Q S N A M T R E F I M L M V P P L T D G F P L D I Query_1 178 AA-CCTGTACTTCC-AATCCAATGCCATGACGCGCGAATTCATCATGCTCATGGTGCCGCCGCTGACGGACGGTTTTCCGCTCGATATTC 265 6 916 ..N...........N........................................................................... 827 5 903 ..-..........--....................................N.N..............-..................... 818 2 126 ..-...........-........................................................................... 213 4 55 ..-...........-........................................................................... 142 3 58 ..-...........-........................................................................... 145 8 882 ..-...........-........................................................................... 795 7 880 ..-...........-........................................................................... 793 1 121 ..-...........-........................................................................... 208 0 1 ................................................................ 64 L V A N V V A C F L L G T V T A L Y A R K I H S R D V H T Query_1 266 TTGTGGCCAATGTGGTCGCCTGTTTTCTCCTCGG-TACGGTGACTGCGCTTTATGCCAGGAAGATTCATTCCC-GCGATGTGCACACCAT 353 6 826 ..................................G...................N..................-................ 738 5 817 .N-................N..............-.......NM.............................C................ 730 2 214 ..................................-......................................-................ 301 4 143 ..................................-......................................-................ 230 3 146 ..................................-......................................-................ 233 8 794 ..................................-......................................-................ 707 7 792 ..................................-......................................-................ 705 1 209 ..................................-......................................-................ 296 0 65 ..................................-......................................-................ 152 I I G T G M M G G V S T F S S F A Y G S V V L A S A S M S A Query_1 354 AATCGG-CACGGGCATGATGGGCGGGGTCTCCACCTTTTCCAGCTTTGCCTATGGCTCGG-TCGTGCTGGCCTCCGCTTCGATGTCGGCT 441 6 737 ......G.....................................................G............................. 648 5 729 ......-.....................................................-.....N....................... 642 2 302 ......-.....................................................-............................. 389 4 231 ......-.....................................................-............................. 318 3 234 ......-.....................................................-............................. 321 8 706 ......-.....................................................-............................. 619 7 704 ......-.....................................................-............................. 617 1 297 ......-.....................................................-............................. 384 0 153 ......-.....................................................-............................. 240 F L I A A A Y V T V S V V A G Y V A V L A G M K F G E K S A Query_1 442 TTTCTCATCGCTGCCGCCTATGTCACGGTGAGCGTGGTTGCTGGTTATGTCGCGGTTCTGGCCGGAATGAAATTCGGTGAGAAAAGTGCA 531 6 647 ...............................N.......................................................... 558 5 641 ...........N.....N.............................N.......................................... 552 2 390 .......................................................................................... 479 4 319 .......................................................................................... 408 3 322 .......................................................................................... 411 8 618 .......................................................................................... 529 7 616 .......................................................................................... 527 1 385 .......................................................................................... 474 0 241 .......................................................................................... 330 D I L H R Y P P M A S I I D S G L V T V E S R H S V A E T I Query_1 532 GATATTCTGCACCGATATCCCCCGATGGCGAGCATTATCGATAGCGGCCTTGTAACGGTCGAGAGCCGCCACTCCGTCGCTGAAACCATC 621 6 557 .......................................................................................... 468 5 551 .......................................................................................... 462 2 480 .......................................................................................... 569 4 409 .......................................................................................... 498 3 412 .......................................................................................... 501 8 528 .......................................................................................... 439 7 526 .......................................................................................... 437 1 475 .......................................................................................... 564 0 331 .......................................................................................... 420 E R V A A K A K S M G M N V F T R V D H G A G A K E A G L G Query_1 622 GAACGTGTCGCGGCGAAGGCGAAGTCGATGGGGATGAATGTTTTCACCCGTGTCGATCACGGGGCCGGAGCCAAAGAGGCGGGGCTGGGC 711 6 467 .......................................................................................... 378 5 461 .......................................................................................... 372 2 570 .......................................................................................... 659 4 499 .......................................................................................... 588 3 502 .......................................................................................... 591 8 438 .......................................................................................... 349 7 436 .......................................................................................... 347 1 565 .......................................................................................... 654 0 421 .......................................................................................... 510 L P P T E L I I F G N P Q N G T V L M Q D K R T I G L D L Query_1 712 TTGCCGCCGACCGAACTCATCATTTTCGGAAACCCGCAAAACGGCACGG-TTTTGATGCAGGACAAGAGAACCATCGG-CCTTG-ATCTT 798 6 377 .................................................-............................-.....-..... 291 5 371 .................................................-............................-.....-..... 285 2 660 .............................................N...-............................-.....-..... 746 4 589 .................................................-............................-.....-..... 675 3 592 .................................................-............................-.....-..... 678 8 348 .................................................-............................-.....-..... 262 7 346 .................................................-............................-.....-..... 260 1 655 .................................................T..........N.................C.....N..... 744 0 511 .................................................-............................-.....-..... 597 P I R A L A W E D G S G K V W L T V N D P A W L A Q R Query_1 799 CCCA-TTCGCGCACTTGCCTGGGAAGACGGC-AGCGGCAA-GG-TCTGGCTCACGGTCAATGA-TCCGG-CCT-GGCTCGCACAACGCCA 881 6 290 ....-..........................-........-..-...................-.....-...-................ 208 5 284 ....-..........................-........-..-...................-.....-...-................ 202 2 747 ....-..........................-........G..-...................-.....-...-................ 830 4 676 ....-..........................-........-..-...................-.....-...-................ 758 3 679 ....-..........................-........-..-...................-.....-...-................ 761 8 261 ....-..........................-........-..-...................-.....-...-................ 179 7 259 ....-..........................-........-..-...................-.....-...-................ 177 1 745 ....T.......................N..A...N....-..T...................T.....AN..G..N............. 833 0 598 ....-..........................-........-..-...................-.....-...-................ 680 H S L G L S S D V A I K A M V T G T G T V T K Y A A G D * Query_1 882 CAG-CCT-GGG--TTTGTCGAGTGACGTGGCTATCAAGGCGATGGTGACTGGAACCGGCACCGTCACGAAATATGCCGCCGGGGATTAAC 967 6 207 ...-...-...--............................................................................. 122 5 201 ...-...-...--............................................................................. 116 2 831 ...-...-...--.........................................N.............-..................... 915 4 759 ...-...-...--............................................................................. 844 3 762 ...-...G...--............................................................................. 848 8 178 ...-...-...--............................................................................. 93 7 176 ...-...-...--............................................................................. 91 1 834 ...T...-...GT......N..... 857 0 681 ...-...-...--......................................................................... 762 H W K W I T D P N S S S V D K L A A A L E H H H H H H * D Query_1 968 ATTGGAAGTGGATAAC--GG-ATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATC 1054 6 121 ................--..-..................................................................... 35 5 115 ................--..-...........G......................................................... 29 2 916 ................--..A.................N......... 961 4 845 ................CT..-TAAA...AC.--...G.TG..--..AT...---------------------.A.G...G...ATG..CT 908 3 849 ................CT..-TAAA...AC.--...G.TG...A.----.TT.AA...-----.....--------------A..-..CT 911 8 92 ................ 77 7 90 ................ 75 P A A N K A R K E A E L A A A T A E Q * L A * P L G A S K R Query_1 1055 CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGG 1144 6 34 ...................... 13 5 28 ..............N....N.. 7 4 909 ..T..A...G.GC..--.TT..TT..C.----.A.................................................-...... 991 3 912 ..T..A...G.GC...---TT.AT..C..-..--................................-...........T........... 994 V L R G F L L K Query_1 1145 TCTTGAGGGGTTTTTTGCTGAAA 1167 4 992 ......-............... 1012 3 995 ....................... 1017 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11487350 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:35 AM Number of letters in database: 10,142 Number of sequences in database: 9 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC114118.102 (APC114118.1-129.F..pMCSG68)
MTPLDVMWVCLGGGVGSLGRWWIGRIVGEYHHGAFPLGTFLINISGAFVIGYLSVLFGVDWHDRYGTMLNAGVLTGILGGYTTFSSMQLDAVKLSHKGQGGLAVFYLVASVLSGLFAAWLGAMLAHLQG
ATGACTCCTTTAGATGTTATGTGGGTCTGTCTGGGCGGAGGTGTCGGATCTCTTGGGCGATGGTGGATAGGGCGTATCGTCGGTGAATATCATCACGGCGCTTTTCCGCTCGGCACGTTTCTCATCAATATTTCCGGGGCGTTCGTCATCGGATATCTGTCGGTGCTGTTTGGTGTCGACTGGCATGACCGTTACGGCACGATGCTGAATGCCGGTGTCCTCACCGGCATACTCGGCGGCTATACGACCTTCAGCAGCATGCAGCTTGATGCCGTCAAACTTTCTCATAAGGGGCAGGGCGGCCTGGCCGTGTTCTATCTGGTTGCGTCCGTCCTTTCCGGCCTGTTTGCCGCATGGCTCGGCGCAATGCTTGCCCATCTTCAGGGT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 9 sequences; 10,172 total letters Query= APC114118.102 Length=792 Score E Sequences producing significant alignments: (Bits) Value 1_01C_C1_APC114118.102AT7sh.seq 1317 0.0 4_06C_C1_APC114118.102AT7term.seq 1247 0.0 5_06D_D1_APC114118.102BT7term.seq 1243 0.0 3_01H_H1_APC114118.102DT7sh.seq 838 0.0 2_01G_G1_APC114118.102CT7sh.seq 838 0.0 7_06H_H1_APC114118.102DT7term.seq 783 0.0 6_06G_G1_APC114118.102CT7term.seq 783 0.0 8_09E_D1_APC114118.102BT7sh.seq 773 0.0 0_wt 699 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L Query_1 1 TCTCGATCCCGCGAAA-TTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAAC-TTT 88 1 5 .............-..............-... 34 4 713 ................A............................................N........................-... 625 4 645 ...G...N.......T.T.C.C.C...........A... 683 5 707 ................-.....................................................................-... 620 5 640 ...G...........T.T.C.C.C...........A... 678 3 12 .....-...........-... 30 2 4 ........G..--................-... 33 7 606 ....-................................................G....................-... 531 7 551 ...C...........T.T.C.C.C...........A... 589 6 605 ....-................................................G....................-... 530 6 550 ...C...........T.T.C.C.C...........A... 588 8 2 ....NN.AN....N.N..-........T..G.N. 34 * E G D I H M H H H H H H S S G V D L W S H P Q F E K Query_1 89 AAGAAGGAGA-TATACAT--ATG-CACCA-TCATCA-TCATCAT-TCTTCTGGTGT-AGATCTGTGGTCTCATCCGCAG-TTCGAAAAGG 169 1 35 ..........-.......--...-.....-......-.......-...........-......................-.......... 115 4 624 ..........-.......--...-.....-......-.......-...........-......................-.......... 544 5 619 ..........-.......--...-.....-......-.......-...........-....N.................-.......... 539 3 31 ..........-.......--... 50 2 34 ..........-.......--... 53 7 530 ..........-.......--... 511 6 529 ..........-.......--... 510 8 35 C.N.......A......NNA...G.....C......G.......N...N....AN.T..........N...N.....N.N.......... 124 G T E N L Y F Q S N A M T P L D V M W V C L G G G V G S L Query_1 170 GTACCGAGAACCTGTACTTCCAATCCAATGCCATGAC-TCC-TTTAGATGTTATGTGGGTCTGTCTGGGCGGAGGTGTCGGATCTCTTGG 257 1 116 .....................................-...-................................................ 203 4 543 .....................................-...-................................................ 456 5 538 .....................................-...-................................................ 451 3 51 ................................-...-................................................ 133 2 54 ................................-...-................................................ 136 7 510 ................................-...-................................................ 428 6 509 ................................-...-................................................ 427 8 125 ..N................N.................G...G...........N..............C........N.N.......... 214 0 1 .....-...-................................................ 56 G R W W I G R I V G E Y H H G A F P L G T F L I N I S G A F Query_1 258 GCGATGGTGGATAGGGCGTATCGTCGGTGAATATCATCACGGCGCTTTTCCGCTCGGCACGTTTCTCATCAATATTTCCGGGGCGTTCGT 347 1 204 .......................................................................................... 293 4 455 .......................................................................................... 366 5 450 .......................................................................................... 361 3 134 .......................................................................................... 223 2 137 .......................................................................................... 226 7 427 .......................................................................................... 338 6 426 .......................................................................................... 337 8 215 ............................N..........................N......N......N....N..N......N....G 304 0 57 .......................................................................................... 146 V I G Y L S V L F G V D W H D R Y G T M L N A G V L T G I L Query_1 348 CATCGGATATCTGTCGGTGCTGTTTGGTGTCGACTGGCATGACCGTTACGGCACGATGCTGAATGCCGGTGTCCTCACCGGCATACTCGG 437 1 294 .......................................................................................... 383 4 365 .......................................................................................... 276 5 360 .......................................................................................... 271 3 224 .......................................................................................... 313 2 227 .......................................................................................... 316 7 337 .......................................................................................... 248 7 767 .............T. 753 7 759 ........... 769 6 336 .......................................................................................... 247 6 766 .............T. 752 6 758 ........... 768 8 305 ..........................N..A...................N........N..........N.................... 394 0 147 .......................................................................................... 236 G G Y T T F S S M Q L D A V K L S H K G Q G G L A V F Y L V Query_1 438 CGGC-TATACGACCTTCAGCAGCATGCAGCTTGATGCCGTCAAA-CTTTCTCATAAGGGGCAGGGCGGCCTGGCCGTGTTCTATCTGGTT 525 1 384 ....-.......................................-............................................. 471 4 275 ....-.......................................-............................................. 188 5 270 ....-.......................................-............................................. 183 3 314 ....-.......................................-............................................. 401 2 317 ....-.......................................-............................................. 404 7 247 ....-.......................................-............................................. 160 7 752 .. 751 6 246 ....-.......................................-............................................. 159 6 751 .. 750 8 395 ....N.....N.........................N..N....N.N.......N.C........N........................ 484 0 237 ....-.......................................-............................................. 324 A S V L S G L F A A W L G A M L A H L Q G * H W K W I T Query_1 526 GCGTCCGTCCTTTCCGGCCTGTTTGCCGCATGGCTC-GGCGCAATGCTTGC-CCATCTTCAGGGTTAACATTGGAAGTGGATAAC--GGA 611 1 472 ....................................-..............-.................................--... 557 4 187 ....................................-..............-.................................--... 102 5 182 ....................................-..............-.................................--... 97 3 402 ....................................-..............-.................................CT..T 489 2 405 ....................................-..............-.................................CT..T 492 7 159 ....................................-..............-................................. 77 6 158 ....................................-..............-................................. 76 8 485 ....N.....N........N.NAN...N....N...NNNNNN.....C...N.......N.A...................N...--... 572 0 325 ....................................-..............-............. 387 D P N S S S V D K L A A A L E H H H H H H * D P A A N K A Query_1 612 TCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTC-GAGcac-caccaccaccaccacTGAGATCCGGCTGCTAA-CAAA--GCC 696 1 558 ........................................-......-................................-....--... 642 4 101 ........................................-......-................................-....--... 17 5 96 ........................................-......-................................T....--NG. 11 3 490 AAA...AC.--...G.TG..--..AT...-----------------------.A.G...G...ATG..CT..T..A...G-.GC.--.-- 547 2 493 AAA...AC.--...G.TG..--..AT...-----------------------.A.G...G...ATG..CT..T..A...G-.GC.--.-- 550 8 573 NN....N..A..N........N........NNT...NT..TN.....TN....N..NN.N......N..A.....NNNN.-.TN.TG... 661 R K E A E L A A A T A E Q * L A * P L G A S K R V L R G Query_1 697 CGAAAGGAAGCTG-AGTTGGCTGCTGCCACCGCTGA-GCAATAA-CTAGCATAACCCCTTGGGG-CCTCTAAACGGGTCTTGAGGGGTTT 782 1 643 .............-......................-.......-...................-......................... 728 4 16 . 16 5 10 .C.... 5 3 548 .TT..TT..C.-----.A..................-.......-...................-......................... 629 2 551 .TT..TT..C.-----.A..................-.......-...................-......................... 632 8 662 .....A....N..A.................NN...NN......NN...........NN...NNC........ 734 F L L K Query_1 783 TTTGCTGAAA 792 1 729 .......... 738 3 630 .......... 639 2 633 .......... 642 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 7764725 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:35 AM Number of letters in database: 10,172 Number of sequences in database: 9 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115038.119 (APC115038.26-783.P(785).D292A.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVTGDITTGQAKSSDVAQKIEKGLVGGLFNLKGVDRILEVQKLAVEKSRLGIPLLFGMDVIHGYETIFPIPLGLSCTWDMAAIEKSARIAAIEASADGISWTFSPMVDISRDPRWGRVSEGSGEDPFLGGAIAQAMVYGYQGANLQDQLHRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEFMYPYEAAVEAGVGSVMASFNEIDGIPATGNKWLLSDLLRGQWGFEGFVVTDFTGISEMIEHGVGDLQTVSALALNAGVDMDMVSEGFVGTLMKSIKEGKVRMGTLNTACRRILEAKYKLGLFDNPYKYCDVNRPKRDIFTKEHRDAARKIAGESFVLLKNAPATAQPLAAHSSSPVTASPVLPLKKQGTVAVIGPLGNTRSNMPGTWSVAARLNDYPSLYEGLKEMMAGKVNITYAKGSNLIGDAAYEERATMFGRSLNRDNRTDQELLDEALKIAAGADVIVAALGESSEMSGESSSRTELGLPDVQHTLLEALLKTGKPVVLTLFTGRPLTLNWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLAAGKWFEKFRSNYLDVDNEPLYPFGYGLSYTTFQYSDIALSTPTLGKDGSVTAVVTVTNTGKHDGAEVVQLYIRDLVGSITRPVRELKGFNKIFLRAGESKTVSFTITRDLLRFYDYDLNYVAEPGDFDIMIGGNSQAVKTAKLTL
AAGTCACCGCAAGACATGGATCGCTTCATCGACGCACTGATGAAGAAGATGACCGTGGAAGAGAAAATCGGACAATTGAACCTACCCGTCACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCACAAAAGATTGAAAAAGGATTGGTGGGCGGACTCTTCAACCTAAAAGGTGTAGACCGTATTCTTGAAGTGCAAAAGCTGGCAGTAGAGAAATCACGCCTCGGTATTCCCCTGCTGTTCGGCATGGATGTGATACATGGCTACGAAACCATCTTCCCCATTCCATTGGGATTGTCCTGCACCTGGGATATGGCGGCTATCGAGAAATCCGCCCGTATTGCAGCCATCGAAGCAAGTGCCGATGGCATTTCCTGGACATTCAGTCCGATGGTAGACATCAGTCGCGACCCACGTTGGGGACGTGTCAGCGAGGGCTCGGGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTATACGGATACCAGGGTGCCAATCTGCAAGACCAATTGCACCGCAACGATGAAATCATGGCTTGCGTAAAACACTTTGCATTGTATGGAGCCGGAGAAGCCGGACGCGACTATAATACAGTAGATATGAGCCGCAACCGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAGGCCGGAGTGGGTAGTGTGATGGCGTCATTCAATGAAATAGACGGTATTCCCGCCACCGGAAACAAATGGCTGCTGAGCGATTTGCTGCGTGGCCAGTGGGGCTTCGAAGGGTTTGTGGTAACGGACTTCACAGGCATTTCAGAGATGATAGAGCATGGTGTCGGCGACTTGCAAACCGTCAGTGCACTCGCTCTTAATGCAGGGGTGGACATGGATATGGTAAGTGAGGGCTTCGTCGGTACACTGATGAAATCAATTAAAGAAGGAAAAGTAAGAATGGGCACGTTGAATACAGCCTGCCGCCGGATATTGGAAGCGAAATACAAGCTGGGACTGTTTGACAATCCTTATAAATACTGCGACGTGAACCGTCCGAAGCGGGATATCTTCACAAAAGAGCATCGTGACGCCGCCCGCAAGATTGCCGGCGAAAGTTTTGTTCTTCTGAAGAATGCCCCCGCCACCGCACAGCCACTCGCAGCTCATAGCTCGTCACCCGTAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAAGGTACAGTTGCCGTCATCGGCCCTCTCGGAAATACCCGCAGCAACATGCCGGGCACCTGGAGCGTAGCCGCACGCCTCAACGATTATCCTTCTTTATACGAAGGCTTGAAAGAAATGATGGCAGGCAAGGTGAACATCACCTATGCCAAAGGTAGTAACCTCATCGGCGATGCAGCTTACGAAGAACGTGCCACCATGTTCGGCCGTTCATTGAACCGCGATAATCGCACGGACCAGGAGTTACTGGACGAAGCACTGAAAATTGCAGCCGGCGCCGATGTTATCGTAGCTGCCCTGGGAGAATCTTCTGAAATGAGCGGTGAAAGTTCAAGCCGCACCGAACTCGGCTTGCCCGATGTACAACATACCCTGTTGGAAGCCTTACTGAAAACGGGTAAACCCGTAGTACTAACCCTCTTTACCGGTCGGCCGTTGACGCTGAACTGGGAACAGGAGCATGTACCTGCCATCTTGAATGTATGGTTCGGAGGCAGTGAAGCGGCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTCAATCCGAGTGGAAAACTAACCATGACTTTCCCGAAGAATGTAGGCCAGATACCTTTGTTCTACAATCATAAGAATACCGGTCGGCCACTGGCGGCAGGCAAATGGTTCGAAAAGTTCCGTTCAAACTATCTGGATGTGGATAACGAACCGCTGTATCCCTTCGGTTATGGATTGTCGTATACCACTTTCCAGTACAGTGACATTGCATTGAGCACACCGACATTGGGAAAAGATGGTTCCGTTACAGCCGTAGTCACCGTCACCAATACTGGTAAACATGACGGTGCGGAAGTAGTTCAACTCTATATCCGCGACCTCGTAGGAAGTATCACCCGCCCTGTACGCGAGTTGAAAGGTTTCAATAAAATCTTCCTTCGCGCCGGAGAAAGCAAAACGGTATCATTCACTATCACGCGTGATCTGCTTCGCTTCTATGATTACGACCTGAACTACGTAGCCGAACCGGGTGACTTTGACATCATGATCGGTGGAAACAGCCAGGCTGTGAAGACGGCGAAGTTGACACTT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,742 total letters Query= APC115038.119 Length=2679 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4093 0.0 K S P Q D M D R F I D A L M K K M T V E E K I G Q L N L P V Query_1 202 AAGTCACCGCAAGACATGGATCGCTTCATCGACGCACTGATGAAGAAGATGACCGTGGAAGAGAAAATCGGACAATTGAACCTACCCGTC 291 0 1 .......................................................................................... 90 T G D I T T G Q A K S S D V A Q K I E K G L V G G L F N L K Query_1 292 ACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCACAAAAGATTGAAAAAGGATTGGTGGGCGGACTCTTCAACCTAAAA 381 0 91 .......................................................................................... 180 G V D R I L E V Q K L A V E K S R L G I P L L F G M D V I H Query_1 382 GGTGTAGACCGTATTCTTGAAGTGCAAAAGCTGGCAGTAGAGAAATCACGCCTCGGTATTCCCCTGCTGTTCGGCATGGATGTGATACAT 471 0 181 .......................................................................................... 270 G Y E T I F P I P L G L S C T W D M A A I E K S A R I A A I Query_1 472 GGCTACGAAACCATCTTCCCCATTCCATTGGGATTGTCCTGCACCTGGGATATGGCGGCTATCGAGAAATCCGCCCGTATTGCAGCCATC 561 0 271 .......................................................................................... 360 E A S A D G I S W T F S P M V D I S R D P R W G R V S E G S Query_1 562 GAAGCAAGTGCCGATGGCATTTCCTGGACATTCAGTCCGATGGTAGACATCAGTCGCGACCCACGTTGGGGACGTGTCAGCGAGGGCTCG 651 0 361 .......................................................................................... 450 G E D P F L G G A I A Q A M V Y G Y Q G A N L Q D Q L H R N Query_1 652 GGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTATACGGATACCAGGGTGCCAATCTGCAAGACCAATTGCACCGCAAC 741 0 451 .......................................................................................... 540 D E I M A C V K H F A L Y G A G E A G R D Y N T V D M S R N Query_1 742 GATGAAATCATGGCTTGCGTAAAACACTTTGCATTGTATGGAGCCGGAGAAGCCGGACGCGACTATAATACAGTAGATATGAGCCGCAAC 831 0 541 .......................................................................................... 630 R M F N E F M Y P Y E A A V E A G V G S V M A S F N E I D G Query_1 832 CGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAGGCCGGAGTGGGTAGTGTGATGGCGTCATTCAATGAAATAGACGGT 921 0 631 .......................................................................................... 720 I P A T G N K W L L S D L L R G Q W G F E G F V V T A F T G Query_1 922 ATTCCCGCCACCGGAAACAAATGGCTGCTGAGCGATTTGCTGCGTGGCCAGTGGGGCTTCGAAGGGTTTGTGGTAACGGCTTTCACAGGC 1011 0 721 ...............................................................................AC......... 810 I S E M I E H G V G D L Q T V S A L A L N A G V D M D M V S Query_1 1012 ATTTCAGAGATGATAGAGCATGGTGTCGGCGACTTGCAAACCGTCAGTGCACTCGCTCTTAATGCAGGGGTGGACATGGATATGGTAAGT 1101 0 811 .......................................................................................... 900 E G F V G T L M K S I K E G K V R M G T L N T A C R R I L E Query_1 1102 GAGGGCTTCGTCGGTACACTGATGAAATCAATTAAAGAAGGAAAAGTAAGAATGGGCACGTTGAATACAGCCTGCCGCCGGATATTGGAA 1191 0 901 .......................................................................................... 990 A K Y K L G L F D N P Y K Y C D V N R P K R D I F T K E H R Query_1 1192 GCGAAATACAAGCTGGGACTGTTTGACAATCCTTATAAATACTGCGACGTGAACCGTCCGAAGCGGGATATCTTCACAAAAGAGCATCGT 1281 0 991 .......................................................................................... 1080 D A A R K I A G E S F V L L K N A P A T A Q P L A A H S S S Query_1 1282 GACGCCGCCCGCAAGATTGCCGGCGAAAGTTTTGTTCTTCTGAAGAATGCCCCCGCCACCGCACAGCCACTCGCAGCTCATAGCTCGTCA 1371 0 1081 .......................................................................................... 1170 P V T A S P V L P L K K Q G T V A V I G P L G N T R S N M P Query_1 1372 CCCGTAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAAGGTACAGTTGCCGTCATCGGCCCTCTCGGAAATACCCGCAGCAACATGCCG 1461 0 1171 .......................................................................................... 1260 G T W S V A A R L N D Y P S L Y E G L K E M M A G K V N I T Query_1 1462 GGCACCTGGAGCGTAGCCGCACGCCTCAACGATTATCCTTCTTTATACGAAGGCTTGAAAGAAATGATGGCAGGCAAGGTGAACATCACC 1551 0 1261 .......................................................................................... 1350 Y A K G S N L I G D A A Y E E R A T M F G R S L N R D N R T Query_1 1552 TATGCCAAAGGTAGTAACCTCATCGGCGATGCAGCTTACGAAGAACGTGCCACCATGTTCGGCCGTTCATTGAACCGCGATAATCGCACG 1641 0 1351 .......................................................................................... 1440 D Q E L L D E A L K I A A G A D V I V A A L G E S S E M S G Query_1 1642 GACCAGGAGTTACTGGACGAAGCACTGAAAATTGCAGCCGGCGCCGATGTTATCGTAGCTGCCCTGGGAGAATCTTCTGAAATGAGCGGT 1731 0 1441 .......................................................................................... 1530 E S S S R T E L G L P D V Q H T L L E A L L K T G K P V V L Query_1 1732 GAAAGTTCAAGCCGCACCGAACTCGGCTTGCCCGATGTACAACATACCCTGTTGGAAGCCTTACTGAAAACGGGTAAACCCGTAGTACTA 1821 0 1531 .......................................................................................... 1620 T L F T G R P L T L N W E Q E H V P A I L N V W F G G S E A Query_1 1822 ACCCTCTTTACCGGTCGGCCGTTGACGCTGAACTGGGAACAGGAGCATGTACCTGCCATCTTGAATGTATGGTTCGGAGGCAGTGAAGCG 1911 0 1621 .......................................................................................... 1710 0 1821 ............ 1832 A Y A I G D V L F G D V N P S G K L T M T F P K N V G Q I P Query_1 1912 GCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTCAATCCGAGTGGAAAACTAACCATGACTTTCCCGAAGAATGTAGGCCAGATACCT 2001 0 1711 .......................................................................................... 1800 L F Y N H K N T G R P L A A G K W F E K F R S N Y L D V D N Query_1 2002 TTGTTCTACAATCATAAGAATACCGGTCGGCCACTGGCGGCAGGCAAATGGTTCGAAAAGTTCCGTTCAAACTATCTGGATGTGGATAAC 2091 0 1801 .......................................................................................... 1890 0 1629 ............ 1640 E P L Y P F G Y G L S Y T T F Q Y S D I A L S T P T L G K D Query_1 2092 GAACCGCTGTATCCCTTCGGTTATGGATTGTCGTATACCACTTTCCAGTACAGTGACATTGCATTGAGCACACCGACATTGGGAAAAGAT 2181 0 1891 .......................................................................................... 1980 G S V T A V V T V T N T G K H D G A E V V Q L Y I R D L V G Query_1 2182 GGTTCCGTTACAGCCGTAGTCACCGTCACCAATACTGGTAAACATGACGGTGCGGAAGTAGTTCAACTCTATATCCGCGACCTCGTAGGA 2271 0 1981 .......................................................................................... 2070 S I T R P V R E L K G F N K I F L R A G E S K T V S F T I T Query_1 2272 AGTATCACCCGCCCTGTACGCGAGTTGAAAGGTTTCAATAAAATCTTCCTTCGCGCCGGAGAAAGCAAAACGGTATCATTCACTATCACG 2361 0 2071 .......................................................................................... 2160 R D L L R F Y D Y D L N Y V A E P G D F D I M I G G N S Q A Query_1 2362 CGTGATCTGCTTCGCTTCTATGATTACGACCTGAACTACGTAGCCGAACCGGGTGACTTTGACATCATGATCGGTGGAAACAGCCAGGCT 2451 0 2161 .......................................................................................... 2250 V K T A K L T L Query_1 2452 GTGAAGACGGCGAAGTTGACACTT 2475 0 2251 ........................ 2274 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 15135768 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:35 AM Number of letters in database: 5,742 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115038.131 (APC115038.26-783.P(785).E536Q.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVTGDITTGQAKSSDVAQKIEKGLVGGLFNLKGVDRILEVQKLAVEKSRLGIPLLFGMDVIHGYETIFPIPLGLSCTWDMAAIEKSARIAAIEASADGISWTFSPMVDISRDPRWGRVSEGSGEDPFLGGAIAQAMVYGYQGANLQDQLHRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEFMYPYEAAVEAGVGSVMASFNEIDGIPATGNKWLLSDLLRGQWGFEGFVVTDFTGISEMIEHGVGDLQTVSALALNAGVDMDMVSEGFVGTLMKSIKEGKVRMGTLNTACRRILEAKYKLGLFDNPYKYCDVNRPKRDIFTKEHRDAARKIAGESFVLLKNAPATAQPLAAHSSSPVTASPVLPLKKQGTVAVIGPLGNTRSNMPGTWSVAARLNDYPSLYEGLKEMMAGKVNITYAKGSNLIGDAAYEERATMFGRSLNRDNRTDQELLDEALKIAAGADVIVAALGESSEMSGESSSRTELGLPDVQHTLLEALLKTGKPVVLTLFTGRPLTLNWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLAAGKWFEKFRSNYLDVDNEPLYPFGYGLSYTTFQYSDIALSTPTLGKDGSVTAVVTVTNTGKHDGAEVVQLYIRDLVGSITRPVRELKGFNKIFLRAGESKTVSFTITRDLLRFYDYDLNYVAEPGDFDIMIGGNSQAVKTAKLTL
AAGTCACCGCAAGACATGGATCGCTTCATCGACGCACTGATGAAGAAGATGACCGTGGAAGAGAAAATCGGACAATTGAACCTACCCGTCACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCACAAAAGATTGAAAAAGGATTGGTGGGCGGACTCTTCAACCTAAAAGGTGTAGACCGTATTCTTGAAGTGCAAAAGCTGGCAGTAGAGAAATCACGCCTCGGTATTCCCCTGCTGTTCGGCATGGATGTGATACATGGCTACGAAACCATCTTCCCCATTCCATTGGGATTGTCCTGCACCTGGGATATGGCGGCTATCGAGAAATCCGCCCGTATTGCAGCCATCGAAGCAAGTGCCGATGGCATTTCCTGGACATTCAGTCCGATGGTAGACATCAGTCGCGACCCACGTTGGGGACGTGTCAGCGAGGGCTCGGGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTATACGGATACCAGGGTGCCAATCTGCAAGACCAATTGCACCGCAACGATGAAATCATGGCTTGCGTAAAACACTTTGCATTGTATGGAGCCGGAGAAGCCGGACGCGACTATAATACAGTAGATATGAGCCGCAACCGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAGGCCGGAGTGGGTAGTGTGATGGCGTCATTCAATGAAATAGACGGTATTCCCGCCACCGGAAACAAATGGCTGCTGAGCGATTTGCTGCGTGGCCAGTGGGGCTTCGAAGGGTTTGTGGTAACGGACTTCACAGGCATTTCAGAGATGATAGAGCATGGTGTCGGCGACTTGCAAACCGTCAGTGCACTCGCTCTTAATGCAGGGGTGGACATGGATATGGTAAGTGAGGGCTTCGTCGGTACACTGATGAAATCAATTAAAGAAGGAAAAGTAAGAATGGGCACGTTGAATACAGCCTGCCGCCGGATATTGGAAGCGAAATACAAGCTGGGACTGTTTGACAATCCTTATAAATACTGCGACGTGAACCGTCCGAAGCGGGATATCTTCACAAAAGAGCATCGTGACGCCGCCCGCAAGATTGCCGGCGAAAGTTTTGTTCTTCTGAAGAATGCCCCCGCCACCGCACAGCCACTCGCAGCTCATAGCTCGTCACCCGTAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAAGGTACAGTTGCCGTCATCGGCCCTCTCGGAAATACCCGCAGCAACATGCCGGGCACCTGGAGCGTAGCCGCACGCCTCAACGATTATCCTTCTTTATACGAAGGCTTGAAAGAAATGATGGCAGGCAAGGTGAACATCACCTATGCCAAAGGTAGTAACCTCATCGGCGATGCAGCTTACGAAGAACGTGCCACCATGTTCGGCCGTTCATTGAACCGCGATAATCGCACGGACCAGGAGTTACTGGACGAAGCACTGAAAATTGCAGCCGGCGCCGATGTTATCGTAGCTGCCCTGGGAGAATCTTCTGAAATGAGCGGTGAAAGTTCAAGCCGCACCGAACTCGGCTTGCCCGATGTACAACATACCCTGTTGGAAGCCTTACTGAAAACGGGTAAACCCGTAGTACTAACCCTCTTTACCGGTCGGCCGTTGACGCTGAACTGGGAACAGGAGCATGTACCTGCCATCTTGAATGTATGGTTCGGAGGCAGTGAAGCGGCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTCAATCCGAGTGGAAAACTAACCATGACTTTCCCGAAGAATGTAGGCCAGATACCTTTGTTCTACAATCATAAGAATACCGGTCGGCCACTGGCGGCAGGCAAATGGTTCGAAAAGTTCCGTTCAAACTATCTGGATGTGGATAACGAACCGCTGTATCCCTTCGGTTATGGATTGTCGTATACCACTTTCCAGTACAGTGACATTGCATTGAGCACACCGACATTGGGAAAAGATGGTTCCGTTACAGCCGTAGTCACCGTCACCAATACTGGTAAACATGACGGTGCGGAAGTAGTTCAACTCTATATCCGCGACCTCGTAGGAAGTATCACCCGCCCTGTACGCGAGTTGAAAGGTTTCAATAAAATCTTCCTTCGCGCCGGAGAAAGCAAAACGGTATCATTCACTATCACGCGTGATCTGCTTCGCTTCTATGATTACGACCTGAACTACGTAGCCGAACCGGGTGACTTTGACATCATGATCGGTGGAAACAGCCAGGCTGTGAAGACGGCGAAGTTGACACTT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,036 total letters Query= APC115038.131 Length=2679 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4093 0.0 1_02B_B2_APC115038.131_B06.seq 21.1 5.8 K S P Q D M D R F I D A L M K K M T V E E K I G Q L N L P V Query_1 202 AAGTCACCGCAAGACATGGATCGCTTCATCGACGCACTGATGAAGAAGATGACCGTGGAAGAGAAAATCGGACAATTGAACCTACCCGTC 291 0 1 .......................................................................................... 90 T G D I T T G Q A K S S D V A Q K I E K G L V G G L F N L K Query_1 292 ACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCACAAAAGATTGAAAAAGGATTGGTGGGCGGACTCTTCAACCTAAAA 381 0 91 .......................................................................................... 180 G V D R I L E V Q K L A V E K S R L G I P L L F G M D V I H Query_1 382 GGTGTAGACCGTATTCTTGAAGTGCAAAAGCTGGCAGTAGAGAAATCACGCCTCGGTATTCCCCTGCTGTTCGGCATGGATGTGATACAT 471 0 181 .......................................................................................... 270 G Y E T I F P I P L G L S C T W D M A A I E K S A R I A A I Query_1 472 GGCTACGAAACCATCTTCCCCATTCCATTGGGATTGTCCTGCACCTGGGATATGGCGGCTATCGAGAAATCCGCCCGTATTGCAGCCATC 561 0 271 .......................................................................................... 360 E A S A D G I S W T F S P M V D I S R D P R W G R V S E G S Query_1 562 GAAGCAAGTGCCGATGGCATTTCCTGGACATTCAGTCCGATGGTAGACATCAGTCGCGACCCACGTTGGGGACGTGTCAGCGAGGGCTCG 651 0 361 .......................................................................................... 450 G E D P F L G G A I A Q A M V Y G Y Q G A N L Q D Q L H R N Query_1 652 GGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTATACGGATACCAGGGTGCCAATCTGCAAGACCAATTGCACCGCAAC 741 0 451 .......................................................................................... 540 D E I M A C V K H F A L Y G A G E A G R D Y N T V D M S R N Query_1 742 GATGAAATCATGGCTTGCGTAAAACACTTTGCATTGTATGGAGCCGGAGAAGCCGGACGCGACTATAATACAGTAGATATGAGCCGCAAC 831 0 541 .......................................................................................... 630 R M F N E F M Y P Y E A A V E A G V G S V M A S F N E I D G Query_1 832 CGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAGGCCGGAGTGGGTAGTGTGATGGCGTCATTCAATGAAATAGACGGT 921 0 631 .......................................................................................... 720 I P A T G N K W L L S D L L R G Q W G F E G F V V T D F T G Query_1 922 ATTCCCGCCACCGGAAACAAATGGCTGCTGAGCGATTTGCTGCGTGGCCAGTGGGGCTTCGAAGGGTTTGTGGTAACGGACTTCACAGGC 1011 0 721 .......................................................................................... 810 1 611 ........... 621 I S E M I E H G V G D L Q T V S A L A L N A G V D M D M V S Query_1 1012 ATTTCAGAGATGATAGAGCATGGTGTCGGCGACTTGCAAACCGTCAGTGCACTCGCTCTTAATGCAGGGGTGGACATGGATATGGTAAGT 1101 0 811 .......................................................................................... 900 E G F V G T L M K S I K E G K V R M G T L N T A C R R I L E Query_1 1102 GAGGGCTTCGTCGGTACACTGATGAAATCAATTAAAGAAGGAAAAGTAAGAATGGGCACGTTGAATACAGCCTGCCGCCGGATATTGGAA 1191 0 901 .......................................................................................... 990 A K Y K L G L F D N P Y K Y C D V N R P K R D I F T K E H R Query_1 1192 GCGAAATACAAGCTGGGACTGTTTGACAATCCTTATAAATACTGCGACGTGAACCGTCCGAAGCGGGATATCTTCACAAAAGAGCATCGT 1281 0 991 .......................................................................................... 1080 D A A R K I A G E S F V L L K N A P A T A Q P L A A H S S S Query_1 1282 GACGCCGCCCGCAAGATTGCCGGCGAAAGTTTTGTTCTTCTGAAGAATGCCCCCGCCACCGCACAGCCACTCGCAGCTCATAGCTCGTCA 1371 0 1081 .......................................................................................... 1170 P V T A S P V L P L K K Q G T V A V I G P L G N T R S N M P Query_1 1372 CCCGTAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAAGGTACAGTTGCCGTCATCGGCCCTCTCGGAAATACCCGCAGCAACATGCCG 1461 0 1171 .......................................................................................... 1260 G T W S V A A R L N D Y P S L Y E G L K E M M A G K V N I T Query_1 1462 GGCACCTGGAGCGTAGCCGCACGCCTCAACGATTATCCTTCTTTATACGAAGGCTTGAAAGAAATGATGGCAGGCAAGGTGAACATCACC 1551 0 1261 .......................................................................................... 1350 Y A K G S N L I G D A A Y E E R A T M F G R S L N R D N R T Query_1 1552 TATGCCAAAGGTAGTAACCTCATCGGCGATGCAGCTTACGAAGAACGTGCCACCATGTTCGGCCGTTCATTGAACCGCGATAATCGCACG 1641 0 1351 .......................................................................................... 1440 D Q E L L D E A L K I A A G A D V I V A A L G E S S E M S G Query_1 1642 GACCAGGAGTTACTGGACGAAGCACTGAAAATTGCAGCCGGCGCCGATGTTATCGTAGCTGCCCTGGGAGAATCTTCTGAAATGAGCGGT 1731 0 1441 .......................................................................................... 1530 Q S S S R T E L G L P D V Q H T L L E A L L K T G K P V V L Query_1 1732 CAGAGTTCAAGCCGCACCGAACTCGGCTTGCCCGATGTACAACATACCCTGTTGGAAGCCTTACTGAAAACGGGTAAACCCGTAGTACTA 1821 0 1531 G.A....................................................................................... 1620 T L F T G R P L T L N W E Q E H V P A I L N V W F G G S E A Query_1 1822 ACCCTCTTTACCGGTCGGCCGTTGACGCTGAACTGGGAACAGGAGCATGTACCTGCCATCTTGAATGTATGGTTCGGAGGCAGTGAAGCG 1911 0 1621 .......................................................................................... 1710 0 1821 ............ 1832 A Y A I G D V L F G D V N P S G K L T M T F P K N V G Q I P Query_1 1912 GCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTCAATCCGAGTGGAAAACTAACCATGACTTTCCCGAAGAATGTAGGCCAGATACCT 2001 0 1711 .......................................................................................... 1800 L F Y N H K N T G R P L A A G K W F E K F R S N Y L D V D N Query_1 2002 TTGTTCTACAATCATAAGAATACCGGTCGGCCACTGGCGGCAGGCAAATGGTTCGAAAAGTTCCGTTCAAACTATCTGGATGTGGATAAC 2091 0 1801 .......................................................................................... 1890 0 1629 ............ 1640 E P L Y P F G Y G L S Y T T F Q Y S D I A L S T P T L G K D Query_1 2092 GAACCGCTGTATCCCTTCGGTTATGGATTGTCGTATACCACTTTCCAGTACAGTGACATTGCATTGAGCACACCGACATTGGGAAAAGAT 2181 0 1891 .......................................................................................... 1980 G S V T A V V T V T N T G K H D G A E V V Q L Y I R D L V G Query_1 2182 GGTTCCGTTACAGCCGTAGTCACCGTCACCAATACTGGTAAACATGACGGTGCGGAAGTAGTTCAACTCTATATCCGCGACCTCGTAGGA 2271 0 1981 .......................................................................................... 2070 S I T R P V R E L K G F N K I F L R A G E S K T V S F T I T Query_1 2272 AGTATCACCCGCCCTGTACGCGAGTTGAAAGGTTTCAATAAAATCTTCCTTCGCGCCGGAGAAAGCAAAACGGTATCATTCACTATCACG 2361 0 2071 .......................................................................................... 2160 R D L L R F Y D Y D L N Y V A E P G D F D I M I G G N S Q A Query_1 2362 CGTGATCTGCTTCGCTTCTATGATTACGACCTGAACTACGTAGCCGAACCGGGTGACTTTGACATCATGATCGGTGGAAACAGCCAGGCT 2451 0 2161 .......................................................................................... 2250 V K T A K L T L Query_1 2452 GTGAAGACGGCGAAGTTGACACTT 2475 0 2251 ........................ 2274 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 13270070 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:35 AM Number of letters in database: 5,036 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115043.112 (APC115043.26-768.P(769).D292A.pMCSG68)
KSGDASMNKFIDKLMDRMTLEEKIGQLNLPSSGDITTGQARSNNIADKIRAGAVGGLFNIKGVEKIQEVQRIAVEESRLKIPLLFGMDVIHGYETVFPIPLGMAATWDMKAIEQSARIAAIEASADGICWTFSPMVDISRDPRWGRVSEGSGEDPFLGGEIAKAMVYGYQGKGDSAYREKTNIMACVKHYALYGAAEAGLDYNTTDMSRIRMFNEYMYPYQAAVDAGAGSVMSSFNEVDGIPATANKWLITDVLRKQWGFGGFVVTDYTGIMEMVNHGIGDMREVSARALSAGVDMDMVSEGYLSTLQQSLKEGKITEKEIDQACRRILEAKYKLGLFDNPYKYCDTERAKTDIYTDEHRSIARRISAESFVLLKNDKQTLPIKKKGKIAVVGPLANTSSNMPGTWSVAVNMEAPATLVEGLKEVAGDKVEIVYAKGSHLMSDAAYEERATLFGRTLYRDKEKRSDIQMLNEALNVAHGADVVVAALGESSEMSGESSSRTDLNIPDVQKTLLEELVKTGKPVVLVLFTGRPLTLTWEDKNVSAILNVWFGGTEAAYAIGDVLFGNVNPGGKLPVTFPQNVGQIPLFYNHKNTGRPLAEGGWFEKFRANYLDVTNEPLYPFGYGLSYAQFDYSDVRLSTDQIDRNGMLTASVTVTNNSECDGDEIVQLYIRDLVGSVTRPVKELKGFEKVTIRAGESKDISFKITPEMLKFYNSDIQFVNEVGEFEVMIGTNSRDVKKATFSL
AAGAGTGGGGATGCGTCGATGAACAAATTTATTGATAAACTGATGGACAGGATGACCTTGGAAGAGAAGATTGGTCAGCTTAATCTTCCCAGCTCGGGAGATATAACCACCGGACAGGCACGCAGCAACAATATTGCAGACAAAATCAGAGCAGGTGCAGTGGGTGGCTTATTCAATATAAAAGGAGTTGAGAAGATACAGGAAGTACAACGTATTGCTGTAGAGGAGAGTCGCCTGAAAATTCCTTTACTCTTTGGCATGGATGTTATTCATGGGTATGAAACTGTTTTCCCTATTCCTTTGGGTATGGCTGCCACATGGGATATGAAGGCTATAGAACAATCTGCTCGTATAGCGGCGATAGAAGCCAGTGCCGATGGCATCTGCTGGACATTTAGTCCGATGGTTGATATCAGCCGTGATCCACGTTGGGGACGTGTATCCGAAGGTAGCGGAGAAGATCCTTTTTTAGGTGGTGAAATTGCTAAGGCGATGGTATATGGCTATCAGGGTAAAGGTGATAGCGCATATCGTGAAAAGACTAATATTATGGCTTGTGTGAAGCACTATGCCTTGTATGGGGCAGCAGAAGCCGGTTTGGACTATAATACAACTGACATGAGCCGTATTCGTATGTTTAATGAATATATGTATCCTTATCAGGCGGCTGTGGATGCGGGTGCCGGCAGTGTCATGTCTTCTTTCAACGAGGTCGATGGAATTCCTGCAACAGCCAACAAATGGTTGATAACTGATGTCCTGCGTAAACAGTGGGGATTCGGTGGTTTTGTCGTTACGGACTATACCGGTATCATGGAAATGGTAAATCATGGTATTGGAGATATGCGAGAAGTCTCTGCCCGTGCTTTGAGTGCAGGAGTGGATATGGATATGGTGAGCGAAGGTTATCTTTCTACACTTCAACAATCATTGAAGGAGGGTAAGATAACAGAGAAAGAGATAGATCAAGCTTGCCGTCGTATTTTGGAGGCAAAATATAAGCTGGGATTATTTGATAATCCTTATAAGTATTGTGATACTGAACGTGCCAAAACGGATATCTACACTGATGAACATCGGAGTATTGCACGCCGGATCTCTGCTGAAAGCTTTGTTCTTTTAAAGAATGATAAACAGACACTGCCTATAAAGAAAAAAGGTAAGATTGCTGTAGTTGGGCCGTTGGCGAATACGAGTTCTAATATGCCCGGAACGTGGAGTGTAGCGGTCAATATGGAAGCTCCAGCTACGCTTGTGGAGGGTTTGAAAGAAGTGGCAGGTGATAAAGTTGAAATTGTGTATGCTAAGGGTAGCCATCTGATGAGTGATGCGGCTTATGAGGAACGTGCAACACTCTTTGGACGTACATTATACCGGGATAAGGAAAAACGTTCCGATATCCAGATGCTGAATGAAGCATTAAATGTTGCTCATGGTGCCGATGTTGTTGTTGCGGCATTAGGTGAATCTTCTGAAATGAGTGGTGAATCGAGTAGTCGAACAGATTTGAATATTCCTGATGTTCAAAAAACATTATTGGAAGAATTAGTGAAAACAGGTAAACCTGTCGTTCTGGTATTATTCACTGGGCGTCCGTTGACCCTGACATGGGAAGACAAAAATGTATCTGCTATTCTGAATGTTTGGTTTGGAGGTACCGAAGCCGCTTATGCTATAGGAGATGTCCTATTCGGAAATGTAAATCCTGGAGGTAAGCTGCCTGTAACATTTCCTCAGAATGTAGGGCAGATTCCTTTATTCTATAACCATAAAAATACTGGACGTCCGCTGGCTGAGGGCGGTTGGTTTGAGAAGTTCCGGGCAAATTATCTGGATGTAACGAATGAACCTCTTTATCCATTTGGCTATGGACTAAGTTATGCACAATTTGATTATAGCGATGTGAGATTAAGTACGGATCAAATAGACCGGAATGGCATGTTAACCGCAAGTGTGACTGTAACCAATAACAGTGAGTGTGATGGAGATGAAATTGTTCAGTTGTATATTCGCGATTTGGTCGGTAGTGTTACTCGTCCGGTGAAAGAATTGAAAGGATTTGAAAAAGTAACAATTAGAGCAGGGGAGTCAAAAGATATTTCTTTTAAGATCACTCCGGAAATGCTTAAGTTCTACAATTCGGATATCCAGTTTGTGAATGAAGTTGGTGAATTCGAAGTAATGATCGGAACGAACAGCAGGGATGTGAAAAAAGCAACGTTTAGCTTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,410 total letters Query= APC115043.112 Length=2634 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4011 0.0 1_02C_C2_APC115043.112_C06.seq 1483 0.0 2_07C_C2_APC115043.112_C08.seq 1328 0.0 E K G T E N L Y F Q S N A K S G D A S M N K F I Query_1 162 CGAAAAGGG---TACCGAG-AACCTGT-ACTT--CCAATCC--AATGCCAAGAGT---GGGGATG-CGT-CGATGAACAAA--TTTATT- 234 0 1 ......---.......-...-...........--......- 33 2 992 .........GGN.C...G.N.......T....TC.......CA.NN........GNGG.......A...T....N...N..AT......N 903 D K L M D R M T L E E K I G Q L N L P S S G D I T Query_1 235 GATAAA-CTGAT-GGAC-AGGATGA-CCTTGG---AAGAGAAGATTGG-TCAG--CTTAAT-CTTCCC-AGCT-CGG-GAG-ATATAACC 309 0 34 ......-.....-....-.......-......---.............-....--......-......-....-...-...-........ 108 2 902 .....NT.NN..T....C.......N....N.GNA.G.N.........N....GCN.....A......C.N..N...T...N....N... 813 T G Q A R S N N I A D K I R A G A V G G L F N I K G Query_1 310 ACC--GGACAGGCA-CGC-AGCAA-CAATA-TTGCAGACAAA-ATC-AG-AGCAGGTGCAGTGGGTGG-CTTATTCAATATAAAA-GGAG 388 0 109 ...--.........-...-.....-.....-...........-...-..-..................-................-.... 187 2 812 CT.CG.........N...C.A...N.....N.........N.N...C..C...............N..G................A.... 723 V E K I Q E V Q R I A V E E S R L K I P L L F G M D V I H Query_1 389 TTGAGAAGATACA-GGAAGTA-CAACGTATTGCTGTAGAGGAGAGTCGCCTGAAAA-TTCCTTTACTCTTTGGCATGGA-TGTTATTCAT 474 0 188 .............-.......-..................................-......................-.......... 273 2 722 ...N.........N.......N..................................A.....................GC.......... 633 G Y E T V F P I P L G M A A T W D M K A I E Q S A R I A A Query_1 475 GGGTATGAAA-CTGTTTTCCCTATTCCTTTGGGTATGGCTGCCACATGGGATATGAAGGCTATAGAACAATCTGCTCGTATAGCGGCGAT 563 0 274 ..........-............................................................................... 362 2 632 ..........A............................................................................... 543 I E A S A D G I C W T F S P M V D I S R D P R W G R V S E G Query_1 564 AGAAGCCAGTGCCGATGGCATCTGCTGGACATTTAGTCCGATGGTTGATATCAGCCGTGATCCACGTTGGGGACGTGTATCCGAAGGTAG 653 0 363 .......................................................................................... 452 2 542 .......................................................................................... 453 S G E D P F L G G E I A K A M V Y G Y Q G K G D S A Y R E K Query_1 654 CGGAGAAGATCCTTTTTTAGGTGGTGAAATTGCTAAGGCGATGGTATATGGCTATCAGGGTAAAGGTGATAGCGCATATCGTGAAAAGAC 743 0 453 .......................................................................................... 542 2 452 .......................................................................................... 363 T N I M A C V K H Y A L Y G A A E A G L D Y N T T D M S R I Query_1 744 TAATATTATGGCTTGTGTGAAGCACTATGCCTTGTATGGGGCAGCAGAAGCCGGTTTGGACTATAATACAACTGACATGAGCCGTATTCG 833 0 543 .......................................................................................... 632 2 362 .......................................................................................... 273 R M F N E Y M Y P Y Q A A V D A G A G S V M S S F N E V D G Query_1 834 TATGTTTAATGAATATATGTATCCTTATCAGGCGGCTGTGGATGCGGGTGCCGGCAGTGTCATGTCTTCTTTCAACGAGGTCGATGGAAT 923 0 633 .......................................................................................... 722 2 272 .......................................................................................... 183 I P A T A N K W L I T D V L R K Q W G F G G F V V T A Y T G Query_1 924 TCCTGCAACAGCCAACAAATGGTTGATAACTGATGTCCTGCGTAAACAGTGGGGATTCGGTGGTTTTGTCGTTACGGCT-TATACCGGTA 1012 0 723 .............................................................................AC-.......... 811 1 9 .......-.....................................................................AC-.......... 96 2 182 .............................................................................A.C.......... 93 I M E M V N H G I G D M R E V S A R A L S A G V D M D M V S Query_1 1013 TCATGGAAATGGTAAATCATGGTATTGGAGATATGCGAGAAGTCTCTGCCCGTGCTTTGAGTGCAGGA-GTGGATATGGATATGGTGAGC 1101 0 812 ....................................................................-..................... 900 1 97 ....................................................................-..................... 185 2 92 ....................................................................T................-N... 4 E G Y L S T L Q Q S L K E G K I T E K E I D Q A C R R I L E Query_1 1102 GAAGGTTATCTTTCTACACTTCAACAATCATTGAAGGAGGGTAAGATAACAGAGAAAGAGATAGATCAAGCTTGCCGTCGTATTTTGGAG 1191 0 901 .......................................................................................... 990 1 186 .......................................................................................... 275 2 3 ... 1 A K Y K L G L F D N P Y K Y C D T E R A K T D I Y T D E H R Query_1 1192 GCAAAATATAAGCTGGGATTATTTGATAATCCTTATAAGTATTGTGATACTGAACGTGCCAAAACGGATATCTACACTGATGAACATCGG 1281 0 991 .......................................................................................... 1080 1 276 .......................................................................................... 365 S I A R R I S A E S F V L L K N D K Q T L P I K K K G K I A Query_1 1282 AGTATTGCACGCCGGATCTCTGCTGAAAGCTTTGTTCTTTTAAAGAATGATAAACAGACACTGCCTATAAAGAAAAAAGGTAAGATTGCT 1371 0 1081 .......................................................................................... 1170 1 366 .......................................................................................... 455 V V G P L A N T S S N M P G T W S V A V N M E A P A T L V E Query_1 1372 GTAGTTGGGCCGTTGGCGAATACGAGTTCTAATATGCCCGGAACGTGGAGTGTAGCGGTCAATATGGAAGCTCCAGCTACGCTTGTGGAG 1461 0 1171 .......................................................................................... 1260 1 456 .......................................................................................... 545 G L K E V A G D K V E I V Y A K G S H L M S D A A Y E E R A Query_1 1462 GGTTTGAAAGAAGTGGCAGGTGATAAAGTTGAAATTGTGTATGCTAAGGGTAGCCATCTGATGAGTGATGCGGCTTATGAGGAACGTGCA 1551 0 1261 .......................................................................................... 1350 1 546 .......................................................................................... 635 T L F G R T L Y R D K E K R S D I Q M L N E A L N V A H G A Query_1 1552 ACACTCTTTGGACGTACATTATACCGGGATAAGGAAAAACGTTCCGATATCCAGATGCTGAATGAAGCATTAAATGTTGCTCATGGTGCC 1641 0 1351 .......................................................................................... 1440 1 636 .......................................................................................... 725 D V V V A A L G E S S E M S G E S S S R T D L N I P D V Q K Query_1 1642 GATGTTGTTGTTGCGGCATTAGGTGAATCTTCTGAAATGAGTGGTGAATCGAGTAGTCGAACAGATTTGAATATTCCTGATGTTCAAAAA 1731 0 1441 .......................................................................................... 1530 1 726 ..........................................................................N............... 815 T L L E E L V K T G K P V V L V L F T G R P L T L T W E D K Query_1 1732 ACATTATTGGAAGAATTAGTGAAAACAGGTAAACCTGTCGTTCTGGTATTATTCACTGGGCGTCCGTTGACCCTGACATGGGAAGACAAA 1821 0 1531 .......................................................................................... 1620 1 816 ..N...............N........... 845 N V S A I L N V W F G G T E A A Y A I G D V L F G N V N P G Query_1 1822 AATGTATCTGCTATTCTGAATGTTTGGTTTGGAGGTACCGAAGCCGCTTATGCTATAGGAGATGTCCTATTCGGAAATGTAAATCCTGGA 1911 0 1621 .......................................................................................... 1710 G K L P V T F P Q N V G Q I P L F Y N H K N T G R P L A E G Query_1 1912 GGTAAGCTGCCTGTAACATTTCCTCAGAATGTAGGGCAGATTCCTTTATTCTATAACCATAAAAATACTGGACGTCCGCTGGCTGAGGGC 2001 0 1711 .......................................................................................... 1800 G W F E K F R A N Y L D V T N E P L Y P F G Y G L S Y A Q F Query_1 2002 GGTTGGTTTGAGAAGTTCCGGGCAAATTATCTGGATGTAACGAATGAACCTCTTTATCCATTTGGCTATGGACTAAGTTATGCACAATTT 2091 0 1801 .......................................................................................... 1890 D Y S D V R L S T D Q I D R N G M L T A S V T V T N N S E C Query_1 2092 GATTATAGCGATGTGAGATTAAGTACGGATCAAATAGACCGGAATGGCATGTTAACCGCAAGTGTGACTGTAACCAATAACAGTGAGTGT 2181 0 1891 .......................................................................................... 1980 D G D E I V Q L Y I R D L V G S V T R P V K E L K G F E K V Query_1 2182 GATGGAGATGAAATTGTTCAGTTGTATATTCGCGATTTGGTCGGTAGTGTTACTCGTCCGGTGAAAGAATTGAAAGGATTTGAAAAAGTA 2271 0 1981 .......................................................................................... 2070 T I R A G E S K D I S F K I T P E M L K F Y N S D I Q F V N Query_1 2272 ACAATTAGAGCAGGGGAGTCAAAAGATATTTCTTTTAAGATCACTCCGGAAATGCTTAAGTTCTACAATTCGGATATCCAGTTTGTGAAT 2361 0 2071 .......................................................................................... 2160 E V G E F E V M I G T N S R D V K K A T F S L Query_1 2362 GAAGTTGGTGAATTCGAAGTAATGATCGGAACGAACAGCAGGGATGTGAAAAAAGCAACGTTTAGCTTG 2430 0 2161 ..................................................................... 2229 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11407503 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:35 AM Number of letters in database: 4,410 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115044.110 (APC115044.33-744.P(748).D104A.pMCSG68)
VESLLSKMTLEEKIGQMNQISSYGNIEDMSALIKKGEIGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQVAKDGARIAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKNVLRDEWGFDGFVVTDWASASEMISHGFAAGSKEVAMKSVNAGVDMEMVSYTFVKELPELVKEGKVKESTIDEAVRNILRIKYRLGLFDTPYVDEQQTSVMYAPSHLEAAKQAAVESAILLKNDKEVLPLQPSVKTVAVVGPMANAPYEQLGTWIFDGEKARTQTPLNAIKEMVGDKVQVIYEPGLAYSREKNPASVAKAAAAAARADVILAFVGEESILSGEAHCLADLDLQGDQGALITALAKTGKPVVTIVMAGRPLTIGKEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKAPVTFPRMVGQIPVYYAHNNTGRPATRNEVLLNDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNIKLASDVLKKDDVLTVTFDLENTGKYEGTEVAQLYIQDKIGSVTRPVKELKRFTRVTLKPGEKKSVSFELPVSELAFWNIDMAKVVEPGDFGLWVATDSQSGEEVFF
GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGTGCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAATCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCGTTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATTGATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGCAGCTATGGTAGAAGGTTTTCAGGGAGATTCGCTGAATAGTCCTACTTCAATTGCAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAGCCGAAGGAGGACGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAGCGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAATGTACTCCGTGACGAGTGGGGATTCGATGGTTTTGTTGTGACGGACTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGTTCAAAAGAAGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTGAAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCTTATGTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTGAAGAATGATAAGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTAGGTACTTGGATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATTTATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTGAATGATATTGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTTGTCGTACACCACATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATTCGATCTGGAAAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGAAAAAAAGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCTTC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,926 total letters Query= APC115044.110 Length=2541 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3847 0.0 2_07D_D2_APC115044.110_D08.seq 884 0.0 1_02D_D2_APC115044.110T7sh.seq 774 0.0 G D I H M H H H H H H S S G V D L W S H P Q F E K G Query_1 94 GGAGATATACATA-TGCACCA-TCATCA-TCATCAT-TCT-TC-TGG-TG-TAGATC-TG-TGG-TCTCATCCGCAGTTCGAAAAGGGTA 172 1 42 ............NC..NN...G......N.N.N...A..NN..A...A..C......N..A...C....N.......N............ 131 T E N L Y F Q S N A V E S L L S K M T L E E K I G Q M N Q Query_1 173 CCGAG-AACC-TG-TACTTCCAATCCAATGCCGTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGA 259 0 1 .......................................................... 58 1 132 .....N....G..A..................................................N......................... 221 I S S Y G N I E D M S A L I K K G E I G S I L N E V D P V Query_1 260 TTTCCTCTTACGGTAATATCGAGGATATGAGTGCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGG-ATCCGGT-GCG 347 0 59 ..............................................................................-.......-... 146 2 703 ..........N.......N.NN 682 1 222 .............G.........................N.................................N....-.......-... 309 R I N A L Q R V A M E E S R L G I P L L I A R A V I H G Query_1 348 TATT--AATGCGCTACA--GCGCGTGGC--AATGGAAGAATCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGCTGTCATTCACGGGTT 431 0 147 ....--...........--.........--............................................A............... 230 2 681 ..N.TA........N.CNG.N.N...N.CA.....N.................N........N.......N.GN.......N.N.N.N.. 592 1 310 ....--.....N.....--........N--...............................N..........N................. 393 F K T I F P I P L G Q A A S F N P Q V A K D G A R I A A I Query_1 432 TAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTT-CGTTCAATCCGCAGGTAGCGA-AAGACGGTGCACGGATAGCAGC-TATTGA 518 0 231 ......................................-.....................-......................-...... 317 2 591 ....N...N......N........N..N.......N..-.....................TN......N..N.....N....NN...N.. 503 1 394 ....N..N..........N...................N.N...N.N.........N...-.N.....A...N........T.-...... 481 E A S S V G I R W T F A P M I D I A R D P R W G R I A E G C Query_1 519 AGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATTGATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGG 608 0 318 .......................................................................................... 407 2 502 ...N.....N.....................N.A....N.........NNN.............N.N.C..................... 413 1 482 ......N......C.C.N...............A.............................T...N...NA...........N..... 571 G E D T Y L T S V M G A A M V E G F Q G D S L N S P T S I Query_1 609 TGAAGAT-AC-GTACCTTACTTCCGTAATGGGAGCAGCTATGGTAGAAGGTTTTCA-GGGAGATTCGCTG-AATAGTCCTACTTCAATTG 694 0 408 .......-..-.............................................-.............-................... 493 2 412 N......G..-............N.N..N............N..............-.......N.....-N.................. 326 1 572 ....C..-..AT............NN.....N....N........T.N........N....N.N...N..A.....C.......NN... 659 A A C P K H F V G Y G A A E G G R D Y N S T F I P E R R L R Query_1 695 CAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAGCCGAAGGAGGACGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCA 784 0 494 .......................................................................................... 583 2 325 ...............................N.......................................................... 236 N V Y L P P F E A A T K A G A A T F M T S F N D N D G I P S Query_1 785 ATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAGCGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTA 874 0 584 .......................................................................................... 673 2 235 ........................................................G................................. 146 T G N A F I L K N V L R D E W G F D G F V V T D W A S A S Query_1 875 CCGGGAATGCTTTTATTTTGAAGAATGTACTCCGTGACGAGTGGGGA-TTCGATGGTTTTGTTGTGA-CGGACTGGGCTTCTGCCAGCGA 962 0 674 ...............................................-...................-...................... 761 2 145 ...............................................G.............N.....C...T....N...N.......N. 56 E M I S H G F A A G S K E V A M K S V N A G V D M E M V S Y Query_1 963 AATGATAAGCCATG-GTTTTGCCGCCGGTTCAAAAGAAGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACA 1051 0 762 ..............-........................................................................... 850 2 55 ..N...G.N...C.AA...NN..C..T.... 25 T F V K E L P E L V K E G K V K E S T I D E A V R N I L R I Query_1 1052 CTTTTGTGAAGGAACTGCCGGAATTAGTGAAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAA 1141 0 851 .......................................................................................... 940 K Y R L G L F D T P Y V D E Q Q T S V M Y A P S H L E A A K Query_1 1142 AGTATCGTTTAGGATTGTTTGATACACCTTATGTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGC 1231 0 941 .......................................................................................... 1030 Q A A V E S A I L L K N D K E V L P L Q P S V K T V A V V G Query_1 1232 AAGCCGCTGTTGAATCGGCTATTCTGTTGAAGAATGATAAGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGAC 1321 0 1031 .......................................................................................... 1120 P M A N A P Y E Q L G T W I F D G E K A R T Q T P L N A I K Query_1 1322 CTATGGCTAATGCACCTTATGAACAGTTAGGTACTTGGATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAG 1411 0 1121 .......................................................................................... 1210 E M V G D K V Q V I Y E P G L A Y S R E K N P A S V A K A A Query_1 1412 AAATGGTTGGCGATAAAGTACAGGTGATTTATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTG 1501 0 1211 .......................................................................................... 1300 A A A A R A D V I L A F V G E E S I L S G E A H C L A D L D Query_1 1502 CCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATT 1591 0 1301 .......................................................................................... 1390 L Q G D Q G A L I T A L A K T G K P V V T I V M A G R P L T Query_1 1592 TGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTA 1681 0 1391 .......................................................................................... 1480 I G K E V E E S T A V L Y S F H P G T M G G P A L A D L L W Query_1 1682 TCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGG 1771 0 1481 .......................................................................................... 1570 G K A V P S G K A P V T F P R M V G Q I P V Y Y A H N N T G Query_1 1772 GGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGAC 1861 0 1571 .......................................................................................... 1660 R P A T R N E V L L N D I A V E A G Q T S L G C T S F Y M D Query_1 1862 GTCCGGCTACACGGAATGAAGTGTTGCTGAATGATATTGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATG 1951 0 1661 .......................................................................................... 1750 A G F D P L F P F G Y G L S Y T T F K Y S N I K L A S D V L Query_1 1952 CGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTTGTCGTACACCACATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGA 2041 0 1751 .......................................................................................... 1840 K K D D V L T V T F D L E N T G K Y E G T E V A Q L Y I Q D Query_1 2042 AAAAAGATGATGTGCTGACAGTGACATTCGATCTGGAAAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATA 2131 0 1841 .......................................................................................... 1930 K I G S V T R P V K E L K R F T R V T L K P G E K K S V S F Query_1 2132 AGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGaaaaaaaGCGTTTCGTTTG 2221 0 1931 .......................................................................................... 2020 E L P V S E L A F W N I D M A K V V E P G D F G L W V A T D Query_1 2222 AACTCCCTGTTAGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATA 2311 0 2021 .......................................................................................... 2110 S Q S G E E V F F Query_1 2312 GTCAGTCCGGAGAAGAAGTTTTCTTC 2337 0 2111 .......................... 2136 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12304500 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:35 AM Number of letters in database: 4,926 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115044.117 (APC115044.33-744.P(748).W418F.pMCSG68)
VESLLSKMTLEEKIGQMNQISSYGNIEDMSALIKKGEIGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQVAKDGARIAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKNVLRDEWGFDGFVVTDWASASEMISHGFAAGSKEVAMKSVNAGVDMEMVSYTFVKELPELVKEGKVKESTIDEAVRNILRIKYRLGLFDTPYVDEQQTSVMYAPSHLEAAKQAAVESAILLKNDKEVLPLQPSVKTVAVVGPMANAPYEQLGTWIFDGEKARTQTPLNAIKEMVGDKVQVIYEPGLAYSREKNPASVAKAAAAAARADVILAFVGEESILSGEAHCLADLDLQGDQGALITALAKTGKPVVTIVMAGRPLTIGKEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKAPVTFPRMVGQIPVYYAHNNTGRPATRNEVLLNDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNIKLASDVLKKDDVLTVTFDLENTGKYEGTEVAQLYIQDKIGSVTRPVKELKRFTRVTLKPGEKKSVSFELPVSELAFWNIDMAKVVEPGDFGLWVATDSQSGEEVFF
GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGTGCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAATCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCGTTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATTGATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGCAGCTATGGTAGAAGGTTTTCAGGGAGATTCGCTGAATAGTCCTACTTCAATTGCAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAGCCGAAGGAGGACGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAGCGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAATGTACTCCGTGACGAGTGGGGATTCGATGGTTTTGTTGTGACGGACTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGTTCAAAAGAAGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTGAAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCTTATGTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTGAAGAATGATAAGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTAGGTACTTGGATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATTTATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTGAATGATATTGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTTGTCGTACACCACATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATTCGATCTGGAAAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGAAAAAAAGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCTTC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,427 total letters Query= APC113179.120 Length=1128 Score E Sequences producing significant alignments: (Bits) Value 2_07E_E2_APC115044.117_E08.seq 21.1 2.1 0_wt 21.1 2.1 A A C I D G G I R D T H Q I L N A D F P V F Y E Y R I S N Query_1 608 AAGCCGCTTGTATAGATGGGGGAATACGGGATACACATCAGATTTTGAATGCTGATTTTCCTGTGTTTTACGAATATCGTATATCGAATG 697 0 268 ........... 258 G S L G R C L I T H Y Q I P I K I G D V T I K P G D I I L G Query_1 698 GTAGCTTGGGGCGCTGTTTGATTACTCACTATCAGATTCCAATTAAAATCGGAGATGTAACGATTAAGCCGGGTGATATAATACTTGGAG 787 0 1990 ........... 2000 D I D G V L V V P R E I A Y E V L L R S E E I R E N E K K I Query_1 788 ATATCGATGGAGTTTTAGTTGTACCAAGGGAAATAGCTTATGAAGTGCTTTTACGCTCGGAGGAGATACGTGAAAATGAGAAGAAGATTT 877 F S W V Q N G D S V Q E I T A K * H W K W I T D P N S S S V Query_1 878 TCTCTTGGGTACAAAACGGAGACAGTGTTCAGGAAATTACAGCAAAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCG 967 D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 968 ACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAG 1042 2 718 ........... 708 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4869448 Query= APC113179.121 Length=1140 Score E Sequences producing significant alignments: (Bits) Value 2_07E_E2_APC115044.117_E08.seq 21.1 2.2 0_wt 21.1 2.2 Query_2 608 AAGCCGCTTGTATAGATGGGGGAATACGGGATACACATCAGATTTTGAATGCTGATTTTCCTGTGTTTTACGAATATCGTATATCGAATG 697 0 268 ........... 258 Query_2 698 GTAGCTTGGGGCGCTGTTTGATTACTCACTATCAGATTCCAATTAAAATCGGAGATGTAACGATTAAGCCGGGTGATATAATACTTGGAG 787 0 1990 ........... 2000 Query_2 788 ATATCGATGGAGTTTTAGTTGTACCAAGGGAAATAGCTTATGAAGTGCTTTTACGCTCGGAGGAGATACGTGAAAATGAGAAGAAGATTT 877 Query_2 878 TCTCTTGGGTACAAAACGGAGACAGTGTTCAGGAAATTACAGCAAAAGGTGGTTACTTTTAACATTGGAAGTGGATAACGGATCCGAATT 967 Query_2 968 CGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAG 1054 2 718 ........... 708 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4921996 Query= APC114117.102 Length=1167 Score E Sequences producing significant alignments: (Bits) Value 2_07E_E2_APC115044.117_E08.seq 21.1 2.2 1_02E_E2_APC115044.117_E06.seq 21.1 2.2 0_wt 21.1 2.2 Query_3 230 TGCCGCCGCTGACGGACGGTTTTCCGCTCGATATTCTTGTGGCCAATGTGGTCGCCTGTTTTCTCCTCGGTACGGTGACTGCGCTTTATG 319 1 252 ........... 262 0 1299 ........... 1309 Query_3 320 CCAGGAAGATTCATTCCCGCGATGTGCACACCATAATCGGCACGGGCATGATGGGCGGGGTCTCCACCTTTTCCAGCTTTGCCTATGGCT 409 Query_3 410 CGGTCGTGCTGGCCTCCGCTTCGATGTCGGCTTTTCTCATCGCTGCCGCCTATGTCACGGTGAGCGTGGTTGCTGGTTATGTCGCGGTTC 499 Query_3 500 TGGCCGGAATGAAATTCGGTGAGAAAAGTGCAGATATTCTGCACCGATATCCCCCGATGGCGAGCATTATCGATAGCGGCCTTGTAACGG 589 Query_3 590 TCGAGAGCCGCCACTCCGTCGCTGAAACCATCGAACGTGTCGCGGCGAAGGCGAAGTCGATGGGGATGAATGTTTTCACCCGTGTCGATC 679 2 513 ........... 523 0 731 ........... 721 Query_3 680 ACGGGGCCGGAGCCAAAGAGGCGGGGCTGGGCTTGCCGCCGACCGAACTCATCATTTTCGGAAACCCGCAAAACGGCACGGTTTTGATGC 769 Query_3 770 AGGACAAGAGAACCATCGGCCTTGATCTTCCCATTCGCGCACTTGCCTGGGAAGACGGCAGCGGCAAGGTCTGGCTCACGGTCAATGATC 859 Query_3 860 CGGCCTGGCTCGCACAACGCCACAGCCTGGGTTTGTCGAGTGACGTGGCTATCAAGGCGATGGTGACTGGAACCGGCACCGTCACGAAAT 949 Query_3 950 ATGCCGCCGGGGATTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccacc 1039 Query_3 1040 accaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAG 1081 2 718 ........... 708 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5040229 Query= APC114118.102 Length=792 Score E Sequences producing significant alignments: (Bits) Value 2_07E_E2_APC115044.117_E08.seq 21.1 1.5 Query_4 696 CCGAAAGGAAG 706 2 718 ........... 708 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3398104 Query= APC115038.119 Length=2679 Score E Sequences producing significant alignments: (Bits) Value 0_wt 51.8 3e-09 1_02E_E2_APC115044.117_E06.seq 48.2 4e-08 2_07E_E2_APC115044.117_E08.seq 21.1 5.1 Query_5 247 AAGATGACCGTGGAAGAGAAAATCGGACAATTGAACCTACCCGTCACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCA 336 0 19 .........C.T..G...........T..GA...... 55 Query_5 337 CAAAAGATTGAAAAAGGATTGGTGGGCGGACTCTTCAACCTAAAAGGTGTAGACCGTATTCTTGAAGTGCAAAAGCTGGCAGTAGAGAAA 426 Query_5 427 TCACGCCTCGGTATTCCCCTGCTGTTCGGCATGGATGTGATACATGGCTACGAAACCATCTTCCCCATTCCATTGGGATTGTCCTGCACC 516 Query_5 517 TGGGATATGGCGGCTATCGAGAAATCCGCCCGTATTGCAGCCATCGAAGCAAGTGCCGATGGCATTTCCTGGACATTCAGTCCGATGGTA 606 Query_5 607 GACATCAGTCGCGACCCACGTTGGGGACGTGTCAGCGAGGGCTCGGGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTA 696 Query_5 697 TACGGATACCAGGGTGCCAATCTGCAAGACCAATTGCACCGCAACGATGAAATCATGGCTTGCGTAAAACACTTTGCATTGTATGGAGCC 786 Query_5 787 GGAGAAGCCGGACGCGACTATAATACAGTAGATATGAGCCGCAACCGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAG 876 Query_5 877 GCCGGAGTGGGTAGTGTGATGGCGTCATTCAATGAAATAGACGGTATTCCCGCCACCGGAAACAAATGGCTGCTGAGCGATTTGCTGCGT 966 Query_5 967 GGCCAGTGGGGCTTCGAAGGGTTTGTGGTAACGGCTTTCACAGGCATTTCAGAGATGATAGAGCATGGTGTCGGCGACTTGCAAACCGTC 1056 Query_5 1057 AGTGCACTCGCTCTTAATGCAGGGGTGGACATGGATATGGTAAGTGAGGGCTTCGTCGGTACACTGATGAAATCAATTAAAGAAGGAAAA 1146 Query_5 1147 GTAAGAATGGGCACGTTGAATACAGCCTGCCGCCGGATATTGGAAGCGAAATACAAGCTGGGACTGTTTGACAATCCTTATAAATACTGC 1236 Query_5 1237 GACGTGAACCGTCCGAAGCGGGATATCTTCACAAAAGAGCATCGTGACGCCGCCCGCAAGATTGCCGGCGAAAGTTTTGTTCTTCTGAAG 1326 0 861 ........... 851 2 383 ........... 393 Query_5 1327 AATGCCCCCGCCACCGCACAGCCACTCGCAGCTCATAGCTCGTCACCCGTAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAAGGTACA 1416 Query_5 1417 GTTGCCGTCATCGGCCCTCTCGGAAATACCCGCAGCAACATGCCGGGCACCTGGAGCGTAGCCGCACGCCTCAACGATTATCCTTCTTTA 1506 Query_5 1507 TACGAAGGCTTGAAAGAAATGATGGCAGGCAAGGTGAACATCACCTATGCCAAAGGTAGTAACCTCATCGGCGATGCAGCTTACGAAGAA 1596 Query_5 1597 CGTGCCACCATGTTCGGCCGTTCATTGAACCGCGATAATCGCACGGACCAGGAGTTACTGGACGAAGCACTGAAAATTGCAGCCGGCGCC 1686 Query_5 1687 GATGTTATCGTAGCTGCCCTGGGAGAATCTTCTGAAATGAGCGGTGAAAGTTCAAGCCGCACCGAACTCGGCTTGCCCGATGTACAACAT 1776 Query_5 1777 ACCCTGTTGGAAGCCTTACTGAAAACGGGTAAACCCGTAGT-ACTAACCCTCTTTACCGGTCGGCCGTTGACGCTGAACTGGGAACAGGA 1865 0 1429 ..G...........T.....G.... 1453 1 382 ..G...........T.....G.... 406 Query_5 1866 GCATGTACCTGCCATCTTGAATGTATGGTTCGGAGGCAGTGAAGCGGCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTCAATCCGAG 1955 0 1582 ..... 1586 0 119 ............ 130 1 535 ..... 539 Query_5 1956 TGGAAAACTAACCATGACTTTCCCGAAGAATGTAGGCCAGATACCTTTGTTCTACAATCATAAGAATACCGGTCGGCCACTGGCGGCAGG 2045 0 1587 ......GGCGC.GG.C..........G..TG..G..A..A..T...G...A....GC......T........A..T.. 1664 1 540 ......GGCGC.NG.C..........G..TG..G..A.NA..T...G...A....GC......T........A..T.. 617 Query_5 2046 CAAATGGTTCGAAAAGTTCCGTTCAAACTATCTGGATGTGGATAACGAACCGCTGTATCCCTTCGGTTATGGATTGTCGTATACCACTTT 2135 1 884 .....N....... 896 Query_5 2136 CCAGTACAGTGACATTGCATTGAGCACACCGACATTGGGAAAAGATGGTTCCGTTACAGCCGTAGTCACCGTCACCAATACTGGTAAACA 2225 0 1081 ........... 1091 0 1858 ........... 1868 2 163 ........... 153 Query_5 2226 TGACGGTGCGGAAGTAGTTCAACTCTATATCCGCGACCTCGTAGGAAGTATCACCCGCCCTGTACGCGAGTTGAAAGGTTTCAATAAAAT 2315 Query_5 2316 CTTCCTTCGCGCCGGAGAAAGCAAAACGGTATCATTCACTATCACGCGTGATCTGCTTCGCTTCTATGATTACGACCTGAACTACGTAGC 2405 Query_5 2406 CGAACCGGGTGACTTTGACATCATGATCGGTGGAAACAGCCAGGCTGTGAAGACGGCGAAGTTGACACTTTAACATTGGAAGTGGATAAC 2495 Query_5 2496 GGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCG 2585 2 718 ... 716 Query_5 2586 AAAGGAAG 2593 2 715 ........ 708 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11648912 Query= APC115038.131 Length=2679 Score E Sequences producing significant alignments: (Bits) Value 0_wt 51.8 3e-09 1_02E_E2_APC115044.117_E06.seq 48.2 4e-08 2_07E_E2_APC115044.117_E08.seq 21.1 5.1 Query_6 247 AAGATGACCGTGGAAGAGAAAATCGGACAATTGAACCTACCCGTCACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCA 336 0 19 .........C.T..G...........T..GA...... 55 Query_6 337 CAAAAGATTGAAAAAGGATTGGTGGGCGGACTCTTCAACCTAAAAGGTGTAGACCGTATTCTTGAAGTGCAAAAGCTGGCAGTAGAGAAA 426 Query_6 427 TCACGCCTCGGTATTCCCCTGCTGTTCGGCATGGATGTGATACATGGCTACGAAACCATCTTCCCCATTCCATTGGGATTGTCCTGCACC 516 Query_6 517 TGGGATATGGCGGCTATCGAGAAATCCGCCCGTATTGCAGCCATCGAAGCAAGTGCCGATGGCATTTCCTGGACATTCAGTCCGATGGTA 606 Query_6 607 GACATCAGTCGCGACCCACGTTGGGGACGTGTCAGCGAGGGCTCGGGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTA 696 Query_6 697 TACGGATACCAGGGTGCCAATCTGCAAGACCAATTGCACCGCAACGATGAAATCATGGCTTGCGTAAAACACTTTGCATTGTATGGAGCC 786 Query_6 787 GGAGAAGCCGGACGCGACTATAATACAGTAGATATGAGCCGCAACCGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAG 876 Query_6 877 GCCGGAGTGGGTAGTGTGATGGCGTCATTCAATGAAATAGACGGTATTCCCGCCACCGGAAACAAATGGCTGCTGAGCGATTTGCTGCGT 966 Query_6 967 GGCCAGTGGGGCTTCGAAGGGTTTGTGGTAACGGACTTCACAGGCATTTCAGAGATGATAGAGCATGGTGTCGGCGACTTGCAAACCGTC 1056 Query_6 1057 AGTGCACTCGCTCTTAATGCAGGGGTGGACATGGATATGGTAAGTGAGGGCTTCGTCGGTACACTGATGAAATCAATTAAAGAAGGAAAA 1146 Query_6 1147 GTAAGAATGGGCACGTTGAATACAGCCTGCCGCCGGATATTGGAAGCGAAATACAAGCTGGGACTGTTTGACAATCCTTATAAATACTGC 1236 Query_6 1237 GACGTGAACCGTCCGAAGCGGGATATCTTCACAAAAGAGCATCGTGACGCCGCCCGCAAGATTGCCGGCGAAAGTTTTGTTCTTCTGAAG 1326 0 861 ........... 851 2 383 ........... 393 Query_6 1327 AATGCCCCCGCCACCGCACAGCCACTCGCAGCTCATAGCTCGTCACCCGTAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAAGGTACA 1416 Query_6 1417 GTTGCCGTCATCGGCCCTCTCGGAAATACCCGCAGCAACATGCCGGGCACCTGGAGCGTAGCCGCACGCCTCAACGATTATCCTTCTTTA 1506 Query_6 1507 TACGAAGGCTTGAAAGAAATGATGGCAGGCAAGGTGAACATCACCTATGCCAAAGGTAGTAACCTCATCGGCGATGCAGCTTACGAAGAA 1596 Query_6 1597 CGTGCCACCATGTTCGGCCGTTCATTGAACCGCGATAATCGCACGGACCAGGAGTTACTGGACGAAGCACTGAAAATTGCAGCCGGCGCC 1686 Query_6 1687 GATGTTATCGTAGCTGCCCTGGGAGAATCTTCTGAAATGAGCGGTCAGAGTTCAAGCCGCACCGAACTCGGCTTGCCCGATGTACAACAT 1776 Query_6 1777 ACCCTGTTGGAAGCCTTACTGAAAACGGGTAAACCCGTAGT-ACTAACCCTCTTTACCGGTCGGCCGTTGACGCTGAACTGGGAACAGGA 1865 0 1429 ..G...........T.....G.... 1453 1 382 ..G...........T.....G.... 406 Query_6 1866 GCATGTACCTGCCATCTTGAATGTATGGTTCGGAGGCAGTGAAGCGGCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTCAATCCGAG 1955 0 1582 ..... 1586 0 119 ............ 130 1 535 ..... 539 Query_6 1956 TGGAAAACTAACCATGACTTTCCCGAAGAATGTAGGCCAGATACCTTTGTTCTACAATCATAAGAATACCGGTCGGCCACTGGCGGCAGG 2045 0 1587 ......GGCGC.GG.C..........G..TG..G..A..A..T...G...A....GC......T........A..T.. 1664 1 540 ......GGCGC.NG.C..........G..TG..G..A.NA..T...G...A....GC......T........A..T.. 617 Query_6 2046 CAAATGGTTCGAAAAGTTCCGTTCAAACTATCTGGATGTGGATAACGAACCGCTGTATCCCTTCGGTTATGGATTGTCGTATACCACTTT 2135 1 884 .....N....... 896 Query_6 2136 CCAGTACAGTGACATTGCATTGAGCACACCGACATTGGGAAAAGATGGTTCCGTTACAGCCGTAGTCACCGTCACCAATACTGGTAAACA 2225 0 1081 ........... 1091 0 1858 ........... 1868 2 163 ........... 153 Query_6 2226 TGACGGTGCGGAAGTAGTTCAACTCTATATCCGCGACCTCGTAGGAAGTATCACCCGCCCTGTACGCGAGTTGAAAGGTTTCAATAAAAT 2315 Query_6 2316 CTTCCTTCGCGCCGGAGAAAGCAAAACGGTATCATTCACTATCACGCGTGATCTGCTTCGCTTCTATGATTACGACCTGAACTACGTAGC 2405 Query_6 2406 CGAACCGGGTGACTTTGACATCATGATCGGTGGAAACAGCCAGGCTGTGAAGACGGCGAAGTTGACACTTTAACATTGGAAGTGGATAAC 2495 Query_6 2496 GGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCG 2585 2 718 ... 716 Query_6 2586 AAAGGAAG 2593 2 715 ........ 708 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11648912 Query= APC115043.112 Length=2634 Score E Sequences producing significant alignments: (Bits) Value 0_wt 69.8 1e-14 2_07E_E2_APC115044.117_E08.seq 46.4 1e-07 1_02E_E2_APC115044.117_E06.seq 22.9 1.4 Query_7 442 ATTCCTTTACTCTTTG-GCATGGATGTTATTCATGGGTATGAAACTGTTTTCCCTATTCCTTTGGGTATGGCTGCCACATGGGATATGAA 530 0 193 .........T.GA.A.C..G..-....C.....C....T.A....AA.............C.....ACAA..G..TT.G.TCA..CC.C. 281 Query_7 531 GGCTATAG-AACAATCTGCTCGTATAGCGGCGATAGAAGCCAGTGCCGATGGCATCTGCTGGACATTTAGTCCGATGGTTGATATCAGCC 619 0 282 ..-..GC.A..G.CGG...A..G.....A..T..T.....TTCGT.T.TA..T...C.G.....T...GCG..A...A.......TGC.. 370 Query_7 620 GTGATCCACGTTGGGGACGTGTATCCGAAGGTAGCGGAGAAGATCCTTTTTTAGGTGGTGAAATTGCTAAGGCGATGGTATATGGCTATC 709 0 371 .C.....T..C.........A.TG.......GT.T..T...... 414 1 1327 ........... 1317 Query_7 710 AGGGTAAAGGTGATAGCGCATATCGTGAAAAGACTAATATTATGGCTTGTGTGAAGCACTATGCCTTGTATGGGGCAGCAGAAGCCGGTT 799 0 1779 ........... 1789 Query_7 800 TGGACTATAATACAACTGACATGAGCCGTATTCGTATGTTTAATGAATATATGTATCCTTATCAGGCGGCTGTGGATGCGGGTGCCGGCA 889 0 1745 ........... 1755 1 699 ........... 709 Query_7 890 GTGTCATGTCTTCTTTCAACGAGGTCGATGGAATTCCTGCAACAGCCAACAAATGGTTGATAACTGATGTCCTGCGTAAACAGTGGGGAT 979 0 698 ....A..C...G.CG......... 721 2 546 ....A..C...G.CG......... 523 Query_7 980 TCGGTGGTTTTGTCGTTACGGCTTATACCGGTATCATGGAAATGGTAAATCATGGTATTGGAGATATGCGAGAAGTCTCTGCCCGTGCTT 1069 0 722 ...A.........T..G.... 742 0 832 ........... 842 2 522 ...A.........T..G.... 502 2 412 ........... 402 Query_7 1070 TGAGTGCAGGAGTGGATATGGATATGGTGAGCGAAGGTTATCTTTCTACACTTCAACAATCATTGAAGGAGGGTAAGATAACAGAGAAAG 1159 Query_7 1160 AGATAGATCAAGCTTGCCGTCGTATTTTGGAGGCAAAATATAAGCTGGGATTATTTGATAATCCTTATAAGTATTGTGATACTGAACGTG 1249 Query_7 1250 CCAAAACGGATATCTACACTGATGAACATCGGAGTATTGCACGCCGGATCTCTGCTGAAAGCTTTGTTCTTTTAAAGAATGATAAACAGA 1339 0 1052 ....G..G........... 1070 2 192 ....G..G........... 174 Query_7 1340 CACTGCCTATAAAGAAAAAAGGTAAGATTGCTGTAGTTGGGCCGTTGGCGAATACGAGTTCTAATATGCCCGGAACGTGGAGTGTAGCGG 1429 Query_7 1430 TCAATATGGAAGCTCCAGCTACGCTTGTGGAGGGTTTGAAAGAAGTGGCAGGTGATAAAGTTGAAATTGTGTATGCTAAGGGTAGCCATC 1519 0 787 ....CA............ 804 2 457 ....CA............ 440 Query_7 1520 TGATGAGTGATGCGGCTTATGAGGAACGTGCAACACTCTTTGGACGTACATTATACCGGGATAAGGAAAAACGTTCCGATATCCAGATGC 1609 Query_7 1610 TGAATGAAGCATTAAATGTTGCTCATGGTGCCGATGTTGTTGTTGCGGCATTAGGTGAATCTTCTGAAATGAGTGGTGAATCGAGTAGTC 1699 Query_7 1700 GAACAGATTTGAATATTCCTGATGTTCAAAAAACATTATTGGAAGAATTAGTGAAAACAGGTAAACCTGTCGTTCTGGTATTATTCACTG 1789 0 1429 ..G..G...........A.. 1448 0 871 ............ 882 2 373 ............ 362 1 382 ..G..G...........A.. 401 Query_7 1790 GGCGTCCGTTGACCCTGACATGGGAAGACAAAAATGTATCTGCTATTCTGAATGTTTGGTTTGGAGGTACCGAAGCCGCTTATGCTATAG 1879 0 270 ........... 260 Query_7 1880 GAGATGTCCTATTCGGAAATGTAAATCCTGGAGGTAAGCTGCCTGTAACATTTCCTCAGAATGTAGGGCAGATTCCTTTATTCTATAACC 1969 0 193 ........... 203 Query_7 1970 ATAAAAATACTGGACGTCCGCTGGCTGAGGGCGGTTGGTTTGAGAAGTTCCGGGCAAATTATCTGGATGTAACGAATGAACCTCTTTATC 2059 Query_7 2060 CATTTGGCTATGGACTAAGTTATGCACAATTTGATTATAGCGATGTGAGATTAAGTACGGATCAAATAGACCGGAATGGCATGTTAACCG 2149 Query_7 2150 CAAGTGTGACTGTAACCAATAACAGTGAGTGTGATGGAGATGAAATTGTTCAGTTGTATATTCGCGATTTGGTCGGTAGTGTTACTCGTC 2239 0 1903 ...G.A.C...A........A.AA...AA.A.T...TCC..G....... 1951 Query_7 2240 CGGTGAAAGAATTGAAAGGATTTGAAAAAGTAACAATTAGAGCAGGGGAGTCAAAAGATATTTCTTTTAAGATCACTCCGGAAATGCTTA 2329 0 1952 ...........C..... 1968 Query_7 2330 AGTTCTACAATTCGGATATCCAGTTTGTGAATGAAGTTGGTGAATTCGAAGTAATGATCGGAACGAACAGCAGGGATGTGAAAAAAGCAA 2419 1 627 ...........T.. 640 Query_7 2420 CGTTTAGCTTGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccacc 2509 Query_7 2510 accacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAG 2548 2 718 ........... 708 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11451992 Query= APC115044.110 Length=2541 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3847 0.0 2_07E_E2_APC115044.117_E08.seq 1357 0.0 1_02E_E2_APC115044.117_E06.seq 1352 0.0 Query_8 202 GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGT 291 0 1 .......................................................................................... 90 Query_8 292 GCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAA 381 0 91 .......................................................................................... 180 Query_8 382 TCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGCTGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCG 471 0 181 ..................................A....................................................... 270 Query_8 472 TTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATT 561 0 271 .......................................................................................... 360 2 912 .... 909 Query_8 562 G-ATATTGCCC-GCGAT-CCT-CGCTGGGG---ACGTATTGCCG-AAGGGTG-TGGTGAAGATACGT-ACCTTA--CTTCCGTAA-TGGG 638 0 361 .-.........-.....-...-........---...........-.......-..............-......--.........-.... 437 2 908 .G..N......C.....N...N....N...GGA....T..N...N.....N.G...N........N.N...N.TNC.........CN..N 819 Query_8 639 AGCAGCTATGGTAGAAGGTTTTCAGGG--AGA-TTCG-CTGAAT-AGTCC-TAC-TTCAATTGCAGCTTGCCCTAAACATTTTGTAGG-T 720 0 438 ...........................--...-....-......-.....-...-.................................-. 519 2 818 ...........................GA...N....G......T.....C...N.................................G. 729 Query_8 721 TACGGTGCAGCCGAAGG--AGGACGTGATTATAATTCC-ACGTTCATTCCCGAA-CGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGA 806 0 520 .................--...................-...............-................................... 605 2 728 ...............A.GA...................C...............A................................N.. 639 Query_8 807 AGCTGCCACCAAAGCGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAA 896 0 606 .......................................................................................... 695 2 638 .......................................................................................... 549 Query_8 897 GAATGTACTCCGTGACGAGTGGGGATTCGATGGTTTTGTTGTGACGGACTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGC 986 0 696 .......................................................................................... 785 2 548 ..............................................................................C.....N..... 459 Query_8 987 CGGTTCAAAAGAAGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATT 1076 0 786 .......................................................................................... 875 2 458 .......................................................................................... 369 Query_8 1077 AGTGAAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATAC 1166 0 876 .......................................................................................... 965 2 368 .......................................................................................... 279 Query_8 1167 ACCTTATGTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCT 1256 0 966 .......................................................................................... 1055 2 278 .......................................................................................... 189 1 2 ....-.N... 10 Query_8 1257 GTTGAAGAATGATAAGGAAGTGTTGCCG-TTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAA- 1344 0 1056 ............................-............................................................- 1143 2 188 ............................-............................................................T 100 1 11 .....C--......-.............C............................................................- 96 Query_8 1345 CAGTTAGGTACTTGGATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAG 1434 0 1144 .......................................................................................... 1233 2 99 .............TT..........................................................-.......-....N... 12 1 97 .............TT........................................................................... 186 Query_8 1435 GTGATTTATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTC 1524 0 1234 .......................................................................................... 1323 0 1318 ............ 1307 2 11 .. 10 1 187 .......................................................................................... 276 1 271 ............ 260 Query_8 1525 ATTCTTGCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTG 1614 0 1324 .......................................................................................... 1413 1 277 .......................................................................................... 366 Query_8 1615 ATTACAGCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCG 1704 0 1414 .......................................................................................... 1503 1 367 .......................................................................................... 456 Query_8 1705 ACTGCTGTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAG 1794 0 1504 .......................................................................................... 1593 1 457 .......................................................................................... 546 Query_8 1795 GCGCCGGTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAG-T 1883 0 1594 ........................................................................................-. 1682 0 1666 ............ 1655 1 547 .....N.........................N........................................................T. 636 1 619 ............ 608 Query_8 1884 GTTGCTGAATGATATTGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGG-TTTTGATCCCTTGTTT- 1971 0 1683 ........................................................................-................- 1770 1 637 ........................................................................T................C 726 Query_8 1972 CCGTTTGGTTAT-GGCTTGTCGTACACCACATTTAAGTAT-AGCAACATCAAACTG-GCGT-CTGATGTACTGAAAAAAGATGATGTGCT 2057 0 1771 ............-...........................-...............-....-............................ 1856 1 727 ............N......N.T........N.....C...A...............C..N.C.N........N................C 816 Query_8 2058 GACAGTGACA-TTCGAT-CTGGAAAATACC--GGGAAATATG-AAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCC 2142 0 1857 ..........-......-............--..........-............................................... 1941 0 1926 ............ 1915 1 817 N..CCGN...N......C............CN..A......NA...... 865 Query_8 2143 GTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGaaaaaaaGCGTTTCGTTTGAACTCCCTGTT 2232 0 1942 .......................................................................................... 2031 Query_8 2233 AGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGA 2322 0 2032 .......................................................................................... 2121 Query_8 2323 GAAGAAGTTTTCTTCTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccac 2412 0 2122 ............... 2136 Query_8 2413 caccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAG 2455 2 718 ........... 708 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11045024 Query= APC115044.117 Length=2541 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3844 0.0 2_07E_E2_APC115044.117_E08.seq 1366 0.0 1_02E_E2_APC115044.117_E06.seq 1361 0.0 Query_9 202 GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGT 291 0 1 .......................................................................................... 90 Query_9 292 GCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAA 381 0 91 .......................................................................................... 180 Query_9 382 TCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCG 471 0 181 .......................................................................................... 270 Query_9 472 TTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATT 561 0 271 .......................................................................................... 360 2 912 .... 909 Query_9 562 G-ATATTGCCC-GCGAT-CCT-CGCTGGGG---ACGTATTGCCG-AAGGGTG-TGGTGAAGATACGT-ACCTTA--CTTCCGTAA-TGGG 638 0 361 .-.........-.....-...-........---...........-.......-..............-......--.........-.... 437 2 908 .G..N......C.....N...N....N...GGA....T..N...N.....N.G...N........N.N...N.TNC.........CN..N 819 Query_9 639 AGCAGCTATGGTAGAAGGTTTTCAGGG--AGA-TTCG-CTGAAT-AGTCC-TAC-TTCAATTGCAGCTTGCCCTAAACATTTTGTAGG-T 720 0 438 ...........................--...-....-......-.....-...-.................................-. 519 2 818 ...........................GA...N....G......T.....C...N.................................G. 729 Query_9 721 TACGGTGCAGCCGAAGG--AGGACGTGATTATAATTCC-ACGTTCATTCCCGAA-CGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGA 806 0 520 .................--...................-...............-................................... 605 2 728 ...............A.GA...................C...............A................................N.. 639 Query_9 807 AGCTGCCACCAAAGCGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAA 896 0 606 .......................................................................................... 695 2 638 .......................................................................................... 549 Query_9 897 GAATGTACTCCGTGACGAGTGGGGATTCGATGGTTTTGTTGTGACGGACTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGC 986 0 696 .......................................................................................... 785 2 548 ..............................................................................C.....N..... 459 Query_9 987 CGGTTCAAAAGAAGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATT 1076 0 786 .......................................................................................... 875 2 458 .......................................................................................... 369 Query_9 1077 AGTGAAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATAC 1166 0 876 .......................................................................................... 965 2 368 .......................................................................................... 279 Query_9 1167 ACCTTATGTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCT 1256 0 966 .......................................................................................... 1055 2 278 .......................................................................................... 189 1 2 ....-.N... 10 Query_9 1257 GTTGAAGAATGATAAGGAAGTGTTGCCG-TTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAA- 1344 0 1056 ............................-............................................................- 1143 2 188 ............................-............................................................T 100 1 11 .....C--......-.............C............................................................- 96 Query_9 1345 CAGTTAGGTACTTTTATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAG 1434 0 1144 .............GG........................................................................... 1233 2 99 .........................................................................-.......-....N... 12 1 97 .......................................................................................... 186 Query_9 1435 GTGATTTATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTC 1524 0 1234 .......................................................................................... 1323 0 1318 ............ 1307 2 11 .. 10 1 187 .......................................................................................... 276 1 271 ............ 260 Query_9 1525 ATTCTTGCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTG 1614 0 1324 .......................................................................................... 1413 1 277 .......................................................................................... 366 Query_9 1615 ATTACAGCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCG 1704 0 1414 .......................................................................................... 1503 1 367 .......................................................................................... 456 Query_9 1705 ACTGCTGTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAG 1794 0 1504 .......................................................................................... 1593 1 457 .......................................................................................... 546 Query_9 1795 GCGCCGGTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAG-T 1883 0 1594 ........................................................................................-. 1682 0 1666 ............ 1655 1 547 .....N.........................N........................................................T. 636 1 619 ............ 608 Query_9 1884 GTTGCTGAATGATATTGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGG-TTTTGATCCCTTGTTT- 1971 0 1683 ........................................................................-................- 1770 1 637 ........................................................................T................C 726 Query_9 1972 CCGTTTGGTTAT-GGCTTGTCGTACACCACATTTAAGTAT-AGCAACATCAAACTG-GCGT-CTGATGTACTGAAAAAAGATGATGTGCT 2057 0 1771 ............-...........................-...............-....-............................ 1856 1 727 ............N......N.T........N.....C...A...............C..N.C.N........N................C 816 Query_9 2058 GACAGTGACA-TTCGAT-CTGGAAAATACC--GGGAAATATG-AAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCC 2142 0 1857 ..........-......-............--..........-............................................... 1941 0 1926 ............ 1915 1 817 N..CCGN...N......C............CN..A......NA...... 865 Query_9 2143 GTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGaaaaaaaGCGTTTCGTTTGAACTCCCTGTT 2232 0 1942 .......................................................................................... 2031 Query_9 2233 AGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGA 2322 0 2032 .......................................................................................... 2121 Query_9 2323 GAAGAAGTTTTCTTCTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccac 2412 0 2122 ............... 2136 Query_9 2413 caccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAG 2455 2 718 ........... 708 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11045024 Query= APC115045.110 Length=2646 Score E Sequences producing significant alignments: (Bits) Value 0_wt 48.2 4e-08 2_07E_E2_APC115044.117_E08.seq 24.7 0.41 1_02E_E2_APC115044.117_E06.seq 22.9 1.4 Query_10 479 AAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATCGAGGCCA 568 0 233 ....A..............C..G.. 257 Query_10 569 GTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAACGGCGAAG 658 0 369 ...C.....T........... 389 Query_10 659 ACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAACGATGAAA 748 Query_10 749 TTATGGCATGTGTAGCTCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAACCGTATGT 838 0 520 ..C.....A.CC....GA...........T...T.. 555 2 708 ...........T...T.. 691 Query_10 839 TCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGCATACCAG 928 Query_10 929 CCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGTATCTCCG 1018 Query_10 1019 AAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGTGAAGGCT 1108 Query_10 1109 TCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAAGCCAAAT 1198 Query_10 1199 ACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGCGACGCTG 1288 Query_10 1289 CTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAAAAAGAAG 1378 0 1080 ............ 1069 2 164 ............ 175 1 32 ........... 22 Query_10 1379 GTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGACTATCCTT 1468 Query_10 1469 CTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCAGCCTACG 1558 Query_10 1559 AAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTGGCCGCTA 1648 Query_10 1649 ATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCCGATGTAC 1738 Query_10 1739 AGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACTTGGGAAC 1828 Query_10 1829 AGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGATGTAAATC 1918 Query_10 1919 CCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCTTTACTTG 2008 Query_10 2009 AAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCGTATACCA 2098 0 1759 .....C....T...G..T..T.....C........C.... 1798 1 884 .....N....... 896 Query_10 2099 ACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTA-TGGGACAGGATG--GTTCTGTTACTGCTATGGTCACGGTAACCAATACCG 2185 0 1799 CA...A.G..T...A....C.AA...-...T.TGATG..C..AA.A.A....AT..G...AC.G..AC..TCGAT.T.G..--....... 1885 1 897 . 897 Query_10 2186 GTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGGTTTG 2275 0 1886 .G..A..T..A..AA.G.......CT...T.G.....A.AA..TAAGA.T..TTC.G.G..T........G............ 1968 Query_10 2276 ATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTCAACT 2365 0 615 ........... 625 2 629 ........... 619 Query_10 2366 ACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTGTAACATTGGAAGT 2455 Query_10 2456 GGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACA 2545 Query_10 2546 AAGCCCGAAAGGAAG 2560 2 718 ........... 708 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11504504 Query= CMR200122.105 Length=2598 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2489 0.0 1_02E_E2_APC115044.117_E06.seq 825 0.0 2_07E_E2_APC115044.117_E08.seq 814 0.0 Query_11 208 GTGGCAAATAAAGGTAGCGATGCGGCAACCGAGAAAAAAGTAGAGTCTCTTTTATCCAGAATGACCCTTGAAGAGAAAATCGGTCAGATG 297 0 1 .....A.....CC.G..T.AG...........G.................. 51 0 598 ............ 587 2 646 ............ 657 Query_11 298 AACCAGATTACCTCTTACGGGAATATTGAGGATATGAGTAGTTTAATTAAGAAAGGTGAAGTCGGGTCTATCCTGAATGAGGTGGATCCG 387 0 52 .........T..........T.....C............GC...G...............A....T..C...T................. 141 0 887 ........... 897 2 357 ........... 347 Query_11 388 GTACGTATTAATGCGTTGCAACGCGTAGCGATGGAGGAGTCCCGGTTGGGAATCCCTTTGTTGATAGCTCGCGCTGTTATTCACGGGTTT 477 0 142 ..G............C.A..G.....G..A.....A..A.....T.....T..T.....A........G..T.A...C............ 231 Query_11 478 AAAACCATTTTTCCCATCCCATTGGGACAAGCGGCTTCGTTCAATCCGCAGATTGCGAAAGACGGTGCACGGGTAGCGGCTATTGAGGCT 567 0 232 .....A.....C..T..T..C..............................G.A..................A....A........A... 321 Query_11 568 TCTTCCGTAGGTATCCGTTGGACTTTTGCACCGATGATCGACATTGCCCGTGAT-CCT-CGCTGGGGG---CGCATTGCCGAA--GGATG 650 0 322 ..G..T...........G...........G..A.....T..T........C...-...-........A---..T.........--..G.. 404 2 899 ....C...N...N....N....GAA..TT..N...N.AG..NG. 856 Query_11 651 TGGTGAAGACACTT-ACC--TGACTTCTGTAA-TGGGAGCTGCCATGGTAGAAGGTTTTCAGGG--AGA-TTCTT-TGAAT-AGTCCC-A 730 0 405 .........T..G.-...--.T.....C....-.......A..T....................--...-...GC-.....-.....T-. 484 2 855 ...N.....T..N.N...NT.NC....C....CN..N...A..T....................GA...N...GGC.....T......T. 766 Query_11 731 C-TTCCATAGCTGCCTGTCCTAAACATTTTGT-GGGCTATGGTGCAGCTGAAGGC--GGACGTGACTATAATTCG-ACATTTATTCCTGA 815 0 485 .-...A..T..A..T..C..............-A..T..C........C.....A--........T........C-..G..C.....C.. 569 2 765 .N...A..T..A..T..C..............A...T..C........C...A.GAA........T........CC..G..C.....C.. 676 Query_11 816 A-CGTCGCCTGCGTAATGTTTACTTGCCACCGTTTGAAGCGGCAACGAAAGCGGGTGCAGCTACGTTTATGACTTCCTTTAATGATAATG 904 0 570 .-.....T.....C........T........T........T..C..C..............C..............A............. 658 2 675 .A.....T.....C........T........T..N.....T..C..C..............C..............A............. 586 Query_11 905 ATGGGATACCCTCTACCGGAAATGCTTTCATATTGAAAGATGTGCTTCGTGGCGAGTGGGGATTTGATGGTTTGGTAGTGACAGACTGGG 994 0 659 ....A..C..T........G........T..T.....GA....A..C....A............C........T..T.....G....... 748 2 585 ....A..C..T........G........T..T.....GA....A..C....A............C........T..T.....G....... 496 Query_11 995 CTTCTGCCAGCGAAATGATAAGTCATGGTTTTGCTGCCGATTCTAAAGAGGTAGCCATGAAATCAGTGAATGCTGGGGTGGATATGGAAA 1084 0 749 ......................C...........C....G...A.....A..G..A........T........A..A..A.......... 838 2 495 ......................C..C.....N..C....G...A.....A..G..A........T........A..A..A.......... 406 Query_11 1085 TGGTAAGTTATACCTTTGTAAAAGAATTGCCTGCATTGATAAAAGAAGGAAAGGTGAAAGAAAGCACCATTGATGAAGCCGTTCGTAATA 1174 0 839 ....G.....C..T.....G..G...C....G.A...AG.G.....G...........G........T..C.....G..T.......... 928 0 102 ........... 112 2 405 ....G.....C..T.....G..G...C....G.A...AG.G.....G...........G........T..C.....G..T.......... 316 Query_11 1175 TATTGCGCGTCAAGTATCGTCTGGGATTGTTTGATGTTCCTTATGTAGATGAAAAGCAACCCTCTGTCATGTATGATCCTTCTCATCTGA 1264 0 929 .T.....TA.A.........T.A............ACA...............C.A...A.A.............C..........T..G 1018 2 315 .T.....TA.A.........T.A............ACA...............C.A...A.A.............C..........T..G 226 Query_11 1265 AAGTAGCTAAGCAGGCTGCTGTAGAATCGGCTATCCTGTTGAAGAATGATAAAGAAGTACTGCCG-TTACAGGAGTCTCTGAAAACCATT 1353 0 1019 ...C.........A..C.....T...........T.................G.....GT.....-......CCA...G.......TG.. 1107 1 9 .......C--......G-.....GT.....C......CCA...G.......TG.. 60 2 225 ...C.........A..C.....T...........T.................G.....GT.....-......CCA...G.......TG.. 137 Query_11 1354 GCTGTGGTAGGACCTATGGCCAATGCGCCTTATGAA-CAATTGGGTACCTGGATCTTTGATGGTGAGAAAGCTCATACTCAGACACCACT 1442 0 1108 ..A.................T.....A.........-..G..A.....T.....A...................G.........T..GT. 1196 1 61 ..A.................T.....A.........-..G..A.....T.TT..A...................G.........T..GT. 149 2 136 ..A.................T.....A.........T..G..A.....T.TT..A...................G.........T..GT. 47 Query_11 1443 GAATGCTATTAAGGAAATAGTTGGCGACAAAGTACAGGTGATTTATGAACCCGGATTAGCTTATAGCCGTGAGAAAAATCCGGCAGGCGT 1532 0 1197 ...C........A.....G........T.......................G...C....A.....T..................A.T.. 1286 1 150 ...C........A.....G........T.......................G...C....A.....T..................A.T.. 239 2 46 ...C........A.....-.G......-T....N..... 10 Query_11 1533 AGCAAAAGCTGCTGCTGTTGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAAGCCATTCTTTCGGGTGAAGCACACTGTCT 1622 0 1287 G..T.....A.....C.CC..........................................T.T.................T......T. 1376 0 1318 ............ 1307 1 240 G..T.....A.....C.CC..........................................T.T.................T......T. 329 1 271 ............ 260 Query_11 1623 GGCAGATTTGAATCTTCAGGGTGATCAAAGTGCTTTGATTACGGCTTTGGCTAAGACAGGTAAACCTGTAGTAACCATTGTGATGGCAGG 1712 0 1377 ...T...C..G..T.G...........GG.A...........A..............G..............G..T...........G.. 1466 0 88 ............. 100 1 330 ...T...C..G..T.G...........GG.A...........A..............G..............G..T...........G.. 419 Query_11 1713 TCGTCCGTTGACTATCGGTCAGGAAGTGGAAGAATCAACAGCTGTTCTTTATTCATTCCATCCGGGTACGATGGGTGGACCGGCATTGGC 1802 0 1467 ...................A.A.....C.....G..G..T........C.................C..A.....C..T..T........ 1556 1 420 ...................A.A.....C.....G..G..T........C.................C..A.....C..T..T........ 509 Query_11 1803 CGATCTGCTGTGGGGTAAGGCGGTTCCAAGTGGAAAAACACCGGTTACTTTCCCGAAGATGGTAGGACAAATTCCGGTATATTATGCTCA 1892 0 1557 T...T....T.....G.....T..G..G........GG.G.....C..........G......G...........T..G..C..C..... 1646 1 510 T...T....T.....G.....T..G..G........GG.G..N..C..........G......G....N......T..G..C..C..... 599 Query_11 1893 TAACAATACCGGGCGGCCGGCTACACGTAATG-AGGTGTTGCTGGATGATATTGCTGTTGAGGCTGGACAAACTTCATTGGGATGTACTT 1981 0 1647 ...T........A..T...........G....-.A.........A...................A.....G......C....C....... 1735 1 600 ...T........A..T...........G....A..T........A...................A.....G......C....C....... 689 Query_11 1982 CTTTCTATATGGATGCCGG-TTTTGAT-CCTTTATTCCCATTTGGCTAT-GGCTTGTCGTATACAACGTTCAAGTAT-AGTAATGTCAAA 2067 0 1736 .C..............G..-.......-..C..G..T..G.....T...-...........C..C..A..T......-..C..CA..... 1821 1 690 .C..............G..T.......C....GT.....G.....T...N......N.T..C..C..N..T..C...A..C..CA..... 779 Query_11 2068 CTTTCATCAGCGTCATTGAAGAAAGATGATGTATTGACTGTGACATTTGATCTGGAAAATACAGGTAAATATAAGGGGACGGAAGTTGCT 2157 0 1822 ..GG.G..T.ATGT.C....A...........GC....A........C..............C..G......G.A..A........A... 1911 1 780 .. 781 Query_11 2158 CAATTGTATATACAAGATAAGGTTGGTTCTGTAACTCGTCCGGTGAAAGAACTGAAACGTTTTACTCGGGTAACCTTGAAACCGGGCGAG 2247 0 1912 .....................A.......C..G..........................C..C.....T..G..A.....G.....T... 2001 0 1926 ............ 1915 Query_11 2248 AAAAAGAATGTTTCGTTTGAACTACCCGTTAGTGAACTTGCATTTTGGAACATCGATATGGTGAAAGTTGTGGAACCCGGAGACTTTGGA 2337 0 2002 .....A.GC..............C..T..........................A.......CT..........................G 2091 Query_11 2338 CTTTGGGTGGCAACAGACAGCCAATCGGGAGAAGAAGTTTTCTTTAAGGTGGTAGATTAACATTGGAAGTGGATAACGGATCCGAATTCG 2427 0 2092 ..............G..T..T..G..C................. 2135 Query_11 2428 AGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAG 2512 2 718 ........... 708 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11294456 Query= CMR200122.108 Length=2598 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2484 0.0 1_02E_E2_APC115044.117_E06.seq 816 0.0 2_07E_E2_APC115044.117_E08.seq 814 0.0 Query_12 208 GTGGCAAATAAAGGTAGCGATGCGGCAACCGAGAAAAAAGTAGAGTCTCTTTTATCCAGAATGACCCTTGAAGAGAAAATCGGTCAGATG 297 0 1 .....A.....CC.G..T.AG...........G.................. 51 0 598 ............ 587 2 646 ............ 657 Query_12 298 AACCAGATTACCTCTTACGGGAATATTGAGGATATGAGTAGTTTAATTAAGAAAGGTGAAGTCGGGTCTATCCTGAATGAGGTGGATCCG 387 0 52 .........T..........T.....C............GC...G...............A....T..C...T................. 141 0 887 ........... 897 2 357 ........... 347 Query_12 388 GTACGTATTAATGCGTTGCAACGCGTAGCGATGGAGGAGTCCCGGTTGGGAATCCCTTTGTTGATAGCTCGCGATGTTATTCACGGGTTT 477 0 142 ..G............C.A..G.....G..A.....A..A.....T.....T..T.....A........G..T.....C............ 231 Query_12 478 AAAACCATTTTTCCCATCCCATTGGGACAAGCGGCTTCGTTCAATCCGCAGATTGCGAAAGACGGTGCACGGGTAGCGGCTATTGAGGCT 567 0 232 .....A.....C..T..T..C..............................G.A..................A....A........A... 321 Query_12 568 TCTTCCGTAGGTATCCGTTGGACTTTTGCACCGATGATCGACATTGCCCGTGAT-CCT-CGCTGGGGG---CGCATTGCCGAA--GGATG 650 0 322 ..G..T...........G...........G..A.....T..T........C...-...-........A---..T.........--..G.. 404 2 899 ....C...N...N....N....GAA..TT..N...N.AG..NG. 856 Query_12 651 TGGTGAAGACACTT-ACC--TGACTTCTGTAA-TGGGAGCTGCCATGGTAGAAGGTTTTCAGGG--AGA-TTCTT-TGAAT-AGTCCC-A 730 0 405 .........T..G.-...--.T.....C....-.......A..T....................--...-...GC-.....-.....T-. 484 2 855 ...N.....T..N.N...NT.NC....C....CN..N...A..T....................GA...N...GGC.....T......T. 766 Query_12 731 C-TTCCATAGCTGCCTGTCCTAAACATTTTGT-GGGCTATGGTGCAGCTGAAGGC--GGACGTGACTATAATTCG-ACATTTATTCCTGA 815 0 485 .-...A..T..A..T..C..............-A..T..C........C.....A--........T........C-..G..C.....C.. 569 2 765 .N...A..T..A..T..C..............A...T..C........C...A.GAA........T........CC..G..C.....C.. 676 Query_12 816 A-CGTCGCCTGCGTAATGTTTACTTGCCACCGTTTGAAGCGGCAACGAAAGCGGGTGCAGCTACGTTTATGACTTCCTTTAATGATAATG 904 0 570 .-.....T.....C........T........T........T..C..C..............C..............A............. 658 2 675 .A.....T.....C........T........T..N.....T..C..C..............C..............A............. 586 Query_12 905 ATGGGATACCCTCTACCGGAAATGCTTTCATATTGAAAGATGTGCTTCGTGGCGAGTGGGGATTTGATGGTTTGGTAGTGACAGACTGGG 994 0 659 ....A..C..T........G........T..T.....GA....A..C....A............C........T..T.....G....... 748 2 585 ....A..C..T........G........T..T.....GA....A..C....A............C........T..T.....G....... 496 Query_12 995 CTTCTGCCAGCGAAATGATAAGTCATGGTTTTGCTGCCGATTCTAAAGAGGTAGCCATGAAATCAGTGAATGCTGGGGTGGATATGGAAA 1084 0 749 ......................C...........C....G...A.....A..G..A........T........A..A..A.......... 838 2 495 ......................C..C.....N..C....G...A.....A..G..A........T........A..A..A.......... 406 Query_12 1085 TGGTAAGTTATACCTTTGTAAAAGAATTGCCTGCATTGATAAAAGAAGGAAAGGTGAAAGAAAGCACCATTGATGAAGCCGTTCGTAATA 1174 0 839 ....G.....C..T.....G..G...C....G.A...AG.G.....G...........G........T..C.....G..T.......... 928 0 102 ........... 112 2 405 ....G.....C..T.....G..G...C....G.A...AG.G.....G...........G........T..C.....G..T.......... 316 Query_12 1175 TATTGCGCGTCAAGTATCGTCTGGGATTGTTTGATGTTCCTTATGTAGATGAAAAGCAACCCTCTGTCATGTATGATCCTTCTCATCTGA 1264 0 929 .T.....TA.A.........T.A............ACA...............C.A...A.A.............C..........T..G 1018 2 315 .T.....TA.A.........T.A............ACA...............C.A...A.A.............C..........T..G 226 Query_12 1265 AAGTAGCTAAGCAGGCTGCTGTAGAATCGGCTATCCTGTTGAAGAATGATAAAGAAGTACTGCCG-TTACAGGAGTCTCTGAAAACCATT 1353 0 1019 ...C.........A..C.....T...........T.................G.....GT.....-......CCA...G.......TG.. 1107 1 9 .......C--......G-.....GT.....C......CCA...G.......TG.. 60 2 225 ...C.........A..C.....T...........T.................G.....GT.....-......CCA...G.......TG.. 137 Query_12 1354 GCTGTGGTAGGACCTATGGCCAATGCGCCTTATGAA-CAATTGGGTACCTGGATCTTTGATGGTGAGAAAGCTCATACTCAGACACCACT 1442 0 1108 ..A.................T.....A.........-..G..A.....T.....A...................G.........T..GT. 1196 1 61 ..A.................T.....A.........-..G..A.....T.TT..A...................G.........T..GT. 149 2 136 ..A.................T.....A.........T..G..A.....T.TT..A...................G.........T..GT. 47 Query_12 1443 GAATGCTATTAAGGAAATAGTTGGCGACAAAGTACAGGTGATTTATGAACCCGGATTAGCTTATAGCCGTGAGAAAAATCCGGCAGGCGT 1532 0 1197 ...C........A.....G........T.......................G...C....A.....T..................A.T.. 1286 1 150 ...C........A.....G........T.......................G...C....A.....T..................A.T.. 239 2 46 ...C........A.....-.G......-T....N..... 10 Query_12 1533 AGCAAAAGCTGCTGCTGTTGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAAGCCATTCTTTCGGGTGCTGCACACTGTCT 1622 0 1287 G..T.....A.....C.CC..........................................T.T.............AA..T......T. 1376 0 1318 ............ 1307 1 240 G..T.....A.....C.CC..........................................T.T.............AA..T......T. 329 1 271 ............ 260 Query_12 1623 GGCAGATTTGAATCTTCAGGGTGATCAAAGTGCTTTGATTACGGCTTTGGCTAAGACAGGTAAACCTGTAGTAACCATTGTGATGGCAGG 1712 0 1377 ...T...C..G..T.G...........GG.A...........A..............G..............G..T...........G.. 1466 0 88 ............. 100 1 330 ...T...C..G..T.G...........GG.A...........A..............G..............G..T...........G.. 419 Query_12 1713 TCGTCCGTTGACTATCGGTCAGGAAGTGGAAGAATCAACAGCTGTTCTTTATTCATTCCATCCGGGTACGATGGGTGGACCGGCATTGGC 1802 0 1467 ...................A.A.....C.....G..G..T........C.................C..A.....C..T..T........ 1556 1 420 ...................A.A.....C.....G..G..T........C.................C..A.....C..T..T........ 509 Query_12 1803 CGATCTGCTGTGGGGTAAGGCGGTTCCAAGTGGAAAAACACCGGTTACTTTCCCGAAGATGGTAGGACAAATTCCGGTATATTATGCTCA 1892 0 1557 T...T....T.....G.....T..G..G........GG.G.....C..........G......G...........T..G..C..C..... 1646 1 510 T...T....T.....G.....T..G..G........GG.G..N..C..........G......G....N......T..G..C..C..... 599 Query_12 1893 TAACAATACCGGGCGGCCGGCTACACGTAATG-AGGTGTTGCTGGATGATATTGCTGTTGAGGCTGGACAAACTTCATTGGGATGTACTT 1981 0 1647 ...T........A..T...........G....-.A.........A...................A.....G......C....C....... 1735 1 600 ...T........A..T...........G....A..T........A...................A.....G......C....C....... 689 Query_12 1982 CTTTCTATATGGATGCCGG-TTTTGAT-CCTTTATTCCCATTTGGCTAT-GGCTTGTCGTATACAACGTTCAAGTAT-AGTAATGTCAAA 2067 0 1736 .C..............G..-.......-..C..G..T..G.....T...-...........C..C..A..T......-..C..CA..... 1821 1 690 .C..............G..T.......C....GT.....G.....T...N......N.T..C..C..N..T..C...A..C..CA..... 779 Query_12 2068 CTTTCATCAGCGTCATTGAAGAAAGATGATGTATTGACTGTGACATTTGATCTGGAAAATACAGGTAAATATAAGGGGACGGAAGTTGCT 2157 0 1822 ..GG.G..T.ATGT.C....A...........GC....A........C..............C..G......G.A..A........A... 1911 1 780 .. 781 Query_12 2158 CAATTGTATATACAAGATAAGGTTGGTTCTGTAACTCGTCCGGTGAAAGAACTGAAACGTTTTACTCGGGTAACCTTGAAACCGGGCGAG 2247 0 1912 .....................A.......C..G..........................C..C.....T..G..A.....G.....T... 2001 0 1926 ............ 1915 Query_12 2248 AAAAAGAATGTTTCGTTTGAACTACCCGTTAGTGAACTTGCATTTTGGAACATCGATATGGTGAAAGTTGTGGAACCCGGAGACTTTGGA 2337 0 2002 .....A.GC..............C..T..........................A.......CT..........................G 2091 Query_12 2338 CTTTGGGTGGCAACAGACAGCCAATCGGGAGAAGAAGTTTTCTTTAAGGTGGTAGATTAACATTGGAAGTGGATAACGGATCCGAATTCG 2427 0 2092 ..............G..T..T..G..C................. 2135 Query_12 2428 AGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAG 2512 2 718 ........... 708 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11294456 Query= CMR200122.112 Length=2598 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2489 0.0 1_02E_E2_APC115044.117_E06.seq 821 0.0 2_07E_E2_APC115044.117_E08.seq 814 0.0 Query_13 208 GTGGCAAATAAAGGTAGCGATGCGGCAACCGAGAAAAAAGTAGAGTCTCTTTTATCCAGAATGACCCTTGAAGAGAAAATCGGTCAGATG 297 0 1 .....A.....CC.G..T.AG...........G.................. 51 0 598 ............ 587 2 646 ............ 657 Query_13 298 AACCAGATTACCTCTTACGGGAATATTGAGGATATGAGTAGTTTAATTAAGAAAGGTGAAGTCGGGTCTATCCTGAATGAGGTGGATCCG 387 0 52 .........T..........T.....C............GC...G...............A....T..C...T................. 141 0 887 ........... 897 2 357 ........... 347 Query_13 388 GTACGTATTAATGCGTTGCAACGCGTAGCGATGGAGGAGTCCCGGTTGGGAATCCCTTTGTTGATAGCTCGCGATGTTATTCACGGGTTT 477 0 142 ..G............C.A..G.....G..A.....A..A.....T.....T..T.....A........G..T.....C............ 231 Query_13 478 AAAACCATTTTTCCCATCCCATTGGGACAAGCGGCTTCGTTCAATCCGCAGATTGCGAAAGACGGTGCACGGGTAGCGGCTATTGAGGCT 567 0 232 .....A.....C..T..T..C..............................G.A..................A....A........A... 321 Query_13 568 TCTTCCGTAGGTATCCGTTGGACTTTTGCACCGATGATCGACATTGCCCGTGAT-CCT-CGCTGGGGG---CGCATTGCCGAA--GGATG 650 0 322 ..G..T...........G...........G..A.....T..T........C...-...-........A---..T.........--..G.. 404 2 899 ....C...N...N....N....GAA..TT..N...N.AG..NG. 856 Query_13 651 TGGTGAAGACACTT-ACC--TGACTTCTGTAA-TGGGAGCTGCCATGGTAGAAGGTTTTCAGGG--AGA-TTCTT-TGAAT-AGTCCC-A 730 0 405 .........T..G.-...--.T.....C....-.......A..T....................--...-...GC-.....-.....T-. 484 2 855 ...N.....T..N.N...NT.NC....C....CN..N...A..T....................GA...N...GGC.....T......T. 766 Query_13 731 C-TTCCATAGCTGCCTGTCCTAAACATTTTGT-GGGCTATGGTGCAGCTGAAGGC--GGACGTGACTATAATTCG-ACATTTATTCCTGA 815 0 485 .-...A..T..A..T..C..............-A..T..C........C.....A--........T........C-..G..C.....C.. 569 2 765 .N...A..T..A..T..C..............A...T..C........C...A.GAA........T........CC..G..C.....C.. 676 Query_13 816 A-CGTCGCCTGCGTAATGTTTACTTGCCACCGTTTGAAGCGGCAACGAAAGCGGGTGCAGCTACGTTTATGACTTCCTTTAATGATAATG 904 0 570 .-.....T.....C........T........T........T..C..C..............C..............A............. 658 2 675 .A.....T.....C........T........T..N.....T..C..C..............C..............A............. 586 Query_13 905 ATGGGATACCCTCTACCGGAAATGCTTTCATATTGAAAGATGTGCTTCGTGGCGAGTGGGGATTTGATGGTTTGGTAGTGACAGACTGGG 994 0 659 ....A..C..T........G........T..T.....GA....A..C....A............C........T..T.....G....... 748 2 585 ....A..C..T........G........T..T.....GA....A..C....A............C........T..T.....G....... 496 Query_13 995 CTTCTGCCAGCGAAATGATAAGTCATGGTTTTGCTGCCGATTCTAAAGAGGTAGCCATGAAATCAGTGAATGCTGGGGTGGATATGGAAA 1084 0 749 ......................C...........C....G...A.....A..G..A........T........A..A..A.......... 838 2 495 ......................C..C.....N..C....G...A.....A..G..A........T........A..A..A.......... 406 Query_13 1085 TGGTAAGTTATACCTTTGTAAAAGAATTGCCTGCATTGATAAAAGAAGGAAAGGTGAAAGAAAGCACCATTGATGAAGCCGTTCGTAATA 1174 0 839 ....G.....C..T.....G..G...C....G.A...AG.G.....G...........G........T..C.....G..T.......... 928 0 102 ........... 112 2 405 ....G.....C..T.....G..G...C....G.A...AG.G.....G...........G........T..C.....G..T.......... 316 Query_13 1175 TATTGCGCGTCAAGTATCGTCTGGGATTGTTTGATGTTCCTTATGTAGATGAAAAGCAACCCTCTGTCATGTATGATCCTTCTCATCTGA 1264 0 929 .T.....TA.A.........T.A............ACA...............C.A...A.A.............C..........T..G 1018 2 315 .T.....TA.A.........T.A............ACA...............C.A...A.A.............C..........T..G 226 Query_13 1265 AAGTAGCTAAGCAGGCTGCTGTAGAATCGGCTATCCTGTTGAAGAATGATAAAGAAGTACTGCCG-TTACAGGAGTCTCTGAAAACCATT 1353 0 1019 ...C.........A..C.....T...........T.................G.....GT.....-......CCA...G.......TG.. 1107 1 9 .......C--......G-.....GT.....C......CCA...G.......TG.. 60 2 225 ...C.........A..C.....T...........T.................G.....GT.....-......CCA...G.......TG.. 137 Query_13 1354 GCTGTGGTAGGACCTATGGCCAATGCGCCTTATGAA-CAATTGGGTACCTGGATCTTTGATGGTGAGAAAGCTCATACTCAGACACCACT 1442 0 1108 ..A.................T.....A.........-..G..A.....T.....A...................G.........T..GT. 1196 1 61 ..A.................T.....A.........-..G..A.....T.TT..A...................G.........T..GT. 149 2 136 ..A.................T.....A.........T..G..A.....T.TT..A...................G.........T..GT. 47 Query_13 1443 GAATGCTATTAAGGAAATAGTTGGCGACAAAGTACAGGTGATTTATGAACCCGGATTAGCTTATAGCCGTGAGAAAAATCCGGCAGGCGT 1532 0 1197 ...C........A.....G........T.......................G...C....A.....T..................A.T.. 1286 1 150 ...C........A.....G........T.......................G...C....A.....T..................A.T.. 239 2 46 ...C........A.....-.G......-T....N..... 10 Query_13 1533 AGCAAAAGCTGCTGCTGTTGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAAGCCATTCTTTCGGGTGAAGCACACTGTCT 1622 0 1287 G..T.....A.....C.CC..........................................T.T.................T......T. 1376 0 1318 ............ 1307 1 240 G..T.....A.....C.CC..........................................T.T.................T......T. 329 1 271 ............ 260 Query_13 1623 GGCAGATTTGAATCTTCAGGGTGATCAAAGTGCTTTGATTACGGCTTTGGCTAAGACAGGTAAACCTGTAGTAACCATTGTGATGGCAGG 1712 0 1377 ...T...C..G..T.G...........GG.A...........A..............G..............G..T...........G.. 1466 0 88 ............. 100 1 330 ...T...C..G..T.G...........GG.A...........A..............G..............G..T...........G.. 419 Query_13 1713 TCGTCCGTTGACTATCGGTCAGGAAGTGGAAGAATCAACAGCTGTTCTTTATTCATTCCATCCGGGTACGATGGGTGGACCGGCATTGGC 1802 0 1467 ...................A.A.....C.....G..G..T........C.................C..A.....C..T..T........ 1556 1 420 ...................A.A.....C.....G..G..T........C.................C..A.....C..T..T........ 509 Query_13 1803 CGATCTGCTGTGGGGTAAGGCGGTTCCAAGTGGAAAAACACCGGTTACTTTCCCGAAGATGGTAGGACAAATTCCGGTATATTATGCTCA 1892 0 1557 T...T....T.....G.....T..G..G........GG.G.....C..........G......G...........T..G..C..C..... 1646 1 510 T...T....T.....G.....T..G..G........GG.G..N..C..........G......G....N......T..G..C..C..... 599 Query_13 1893 TAACAATACCGGGGCTCCGGCTACACGTAATG-AGGTGTTGCTGGATGATATTGCTGTTGAGGCTGGACAAACTTCATTGGGATGTACTT 1981 0 1647 ...T........ACG............G....-.A.........A...................A.....G......C....C....... 1735 1 600 ...T........ACG............G....A..T........A...................A.....G......C....C....... 689 Query_13 1982 CTTTCTATATGGATGCCGG-TTTTGAT-CCTTTATTCCCATTTGGCTAT-GGCTTGTCGTATACAACGTTCAAGTAT-AGTAATGTCAAA 2067 0 1736 .C..............G..-.......-..C..G..T..G.....T...-...........C..C..A..T......-..C..CA..... 1821 1 690 .C..............G..T.......C....GT.....G.....T...N......N.T..C..C..N..T..C...A..C..CA..... 779 Query_13 2068 CTTTCATCAGCGTCATTGAAGAAAGATGATGTATTGACTGTGACATTTGATCTGGAAAATACAGGTAAATATAAGGGGACGGAAGTTGCT 2157 0 1822 ..GG.G..T.ATGT.C....A...........GC....A........C..............C..G......G.A..A........A... 1911 1 780 .. 781 Query_13 2158 CAATTGTATATACAAGATAAGGTTGGTTCTGTAACTCGTCCGGTGAAAGAACTGAAACGTTTTACTCGGGTAACCTTGAAACCGGGCGAG 2247 0 1912 .....................A.......C..G..........................C..C.....T..G..A.....G.....T... 2001 0 1926 ............ 1915 Query_13 2248 AAAAAGAATGTTTCGTTTGAACTACCCGTTAGTGAACTTGCATTTTGGAACATCGATATGGTGAAAGTTGTGGAACCCGGAGACTTTGGA 2337 0 2002 .....A.GC..............C..T..........................A.......CT..........................G 2091 Query_13 2338 CTTTGGGTGGCAACAGACAGCCAATCGGGAGAAGAAGTTTTCTTTAAGGTGGTAGATTAACATTGGAAGTGGATAACGGATCCGAATTCG 2427 0 2092 ..............G..T..T..G..C................. 2135 Query_13 2428 AGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAG 2512 2 718 ........... 708 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11294456 Query= CMR200122.115 Length=2598 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2484 0.0 1_02E_E2_APC115044.117_E06.seq 816 0.0 2_07E_E2_APC115044.117_E08.seq 814 0.0 Query_14 208 GTGGCAAATAAAGGTAGCGATGCGGCAACCGAGAAAAAAGTAGAGTCTCTTTTATCCAGAATGACCCTTGAAGAGAAAATCGGTCAGATG 297 0 1 .....A.....CC.G..T.AG...........G.................. 51 0 598 ............ 587 2 646 ............ 657 Query_14 298 AACCAGATTACCTCTTACGGGAATATTGAGGATATGAGTAGTTTAATTAAGAAAGGTGAAGTCGGGTCTATCCTGAATGAGGTGGATCCG 387 0 52 .........T..........T.....C............GC...G...............A....T..C...T................. 141 0 887 ........... 897 2 357 ........... 347 Query_14 388 GTACGTATTAATGCGTTGCAACGCGTAGCGATGGAGGAGTCCCGGTTGGGAATCCCTTTGTTGATAGCTCGCGATGTTATTCACGGGTTT 477 0 142 ..G............C.A..G.....G..A.....A..A.....T.....T..T.....A........G..T.....C............ 231 Query_14 478 AAAACCATTTTTCCCATCCCATTGGGACAAGCGGCTTCGTTCAATCCGCAGATTGCGAAAGACGGTGCACGGGTAGCGGCTATTGAGGCT 567 0 232 .....A.....C..T..T..C..............................G.A..................A....A........A... 321 Query_14 568 TCTTCCGTAGGTATCCGTTGGACTTTTGCACCGATGATCGACATTGCCCGTGAT-CCT-CGCTGGGGG---CGCATTGCCGAA--GGATG 650 0 322 ..G..T...........G...........G..A.....T..T........C...-...-........A---..T.........--..G.. 404 2 899 ....C...N...N....N....GAA..TT..N...N.AG..NG. 856 Query_14 651 TGGTGAAGACACTT-ACC--TGACTTCTGTAA-TGGGAGCTGCCATGGTAGAAGGTTTTCAGGG--AGA-TTCTT-TGAAT-AGTCCC-A 730 0 405 .........T..G.-...--.T.....C....-.......A..T....................--...-...GC-.....-.....T-. 484 2 855 ...N.....T..N.N...NT.NC....C....CN..N...A..T....................GA...N...GGC.....T......T. 766 Query_14 731 C-TTCCATAGCTGCCTGTCCTAAACATTTTGT-GGGCTATGGTGCAGCTGAAGGC--GGACGTGACTATAATTCG-ACATTTATTCCTGA 815 0 485 .-...A..T..A..T..C..............-A..T..C........C.....A--........T........C-..G..C.....C.. 569 2 765 .N...A..T..A..T..C..............A...T..C........C...A.GAA........T........CC..G..C.....C.. 676 Query_14 816 A-CGTCGCCTGCGTAATGTTTACTTGCCACCGTTTGAAGCGGCAACGAAAGCGGGTGCAGCTACGTTTATGACTTCCTTTAATGATAATG 904 0 570 .-.....T.....C........T........T........T..C..C..............C..............A............. 658 2 675 .A.....T.....C........T........T..N.....T..C..C..............C..............A............. 586 Query_14 905 ATGGGATACCCTCTACCGGAAATGCTTTCATATTGAAAGATGTGCTTCGTGGCGAGTGGGGATTTGATGGTTTGGTAGTGACAGACTGGG 994 0 659 ....A..C..T........G........T..T.....GA....A..C....A............C........T..T.....G....... 748 2 585 ....A..C..T........G........T..T.....GA....A..C....A............C........T..T.....G....... 496 Query_14 995 CTTCTGCCAGCGAAATGATAAGTCATGGTTTTGCTGCCGATTCTAAAGAGGTAGCCATGAAATCAGTGAATGCTGGGGTGGATATGGAAA 1084 0 749 ......................C...........C....G...A.....A..G..A........T........A..A..A.......... 838 2 495 ......................C..C.....N..C....G...A.....A..G..A........T........A..A..A.......... 406 Query_14 1085 TGGTAAGTTATACCTTTGTAAAAGAATTGCCTGCATTGATAAAAGAAGGAAAGGTGAAAGAAAGCACCATTGATGAAGCCGTTCGTAATA 1174 0 839 ....G.....C..T.....G..G...C....G.A...AG.G.....G...........G........T..C.....G..T.......... 928 0 102 ........... 112 2 405 ....G.....C..T.....G..G...C....G.A...AG.G.....G...........G........T..C.....G..T.......... 316 Query_14 1175 TATTGCGCGTCAAGTATCGTCTGGGATTGTTTGATGTTCCTTATGTAGATGAAAAGCAACCCTCTGTCATGTATGATCCTTCTCATCTGA 1264 0 929 .T.....TA.A.........T.A............ACA...............C.A...A.A.............C..........T..G 1018 2 315 .T.....TA.A.........T.A............ACA...............C.A...A.A.............C..........T..G 226 Query_14 1265 AAGTAGCTAAGCAGGCTGCTGTAGAATCGGCTATCCTGTTGAAGAATGATAAAGAAGTACTGCCG-TTACAGGAGTCTCTGAAAACCATT 1353 0 1019 ...C.........A..C.....T...........T.................G.....GT.....-......CCA...G.......TG.. 1107 1 9 .......C--......G-.....GT.....C......CCA...G.......TG.. 60 2 225 ...C.........A..C.....T...........T.................G.....GT.....-......CCA...G.......TG.. 137 Query_14 1354 GCTGTGGTAGGACCTATGGCCAATGCGCCTTATGAA-CAATTGGGTACCTGGATCTTTGATGGTGAGAAAGCTCATACTCAGACACCACT 1442 0 1108 ..A.................T.....A.........-..G..A.....T.....A...................G.........T..GT. 1196 1 61 ..A.................T.....A.........-..G..A.....T.TT..A...................G.........T..GT. 149 2 136 ..A.................T.....A.........T..G..A.....T.TT..A...................G.........T..GT. 47 Query_14 1443 GAATGCTATTAAGGAAATAGTTGGCGACAAAGTACAGGTGATTTATGAACCCGGATTAGCTTATAGCCGTGAGAAAAATCCGGCAGGCGT 1532 0 1197 ...C........A.....G........T.......................G...C....A.....T..................A.T.. 1286 1 150 ...C........A.....G........T.......................G...C....A.....T..................A.T.. 239 2 46 ...C........A.....-.G......-T....N..... 10 Query_14 1533 AGCAAAAGCTGCTGCTGTTGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAAGCCATTCTTTCGGGTCAGGCACACTGTCT 1622 0 1287 G..T.....A.....C.CC..........................................T.T............G.A..T......T. 1376 0 1318 ............ 1307 1 240 G..T.....A.....C.CC..........................................T.T............G.A..T......T. 329 1 271 ............ 260 Query_14 1623 GGCAGATTTGAATCTTCAGGGTGATCAAAGTGCTTTGATTACGGCTTTGGCTAAGACAGGTAAACCTGTAGTAACCATTGTGATGGCAGG 1712 0 1377 ...T...C..G..T.G...........GG.A...........A..............G..............G..T...........G.. 1466 0 88 ............. 100 1 330 ...T...C..G..T.G...........GG.A...........A..............G..............G..T...........G.. 419 Query_14 1713 TCGTCCGTTGACTATCGGTCAGGAAGTGGAAGAATCAACAGCTGTTCTTTATTCATTCCATCCGGGTACGATGGGTGGACCGGCATTGGC 1802 0 1467 ...................A.A.....C.....G..G..T........C.................C..A.....C..T..T........ 1556 1 420 ...................A.A.....C.....G..G..T........C.................C..A.....C..T..T........ 509 Query_14 1803 CGATCTGCTGTGGGGTAAGGCGGTTCCAAGTGGAAAAACACCGGTTACTTTCCCGAAGATGGTAGGACAAATTCCGGTATATTATGCTCA 1892 0 1557 T...T....T.....G.....T..G..G........GG.G.....C..........G......G...........T..G..C..C..... 1646 1 510 T...T....T.....G.....T..G..G........GG.G..N..C..........G......G....N......T..G..C..C..... 599 Query_14 1893 TAACAATACCGGGCGGCCGGCTACACGTAATG-AGGTGTTGCTGGATGATATTGCTGTTGAGGCTGGACAAACTTCATTGGGATGTACTT 1981 0 1647 ...T........A..T...........G....-.A.........A...................A.....G......C....C....... 1735 1 600 ...T........A..T...........G....A..T........A...................A.....G......C....C....... 689 Query_14 1982 CTTTCTATATGGATGCCGG-TTTTGAT-CCTTTATTCCCATTTGGCTAT-GGCTTGTCGTATACAACGTTCAAGTAT-AGTAATGTCAAA 2067 0 1736 .C..............G..-.......-..C..G..T..G.....T...-...........C..C..A..T......-..C..CA..... 1821 1 690 .C..............G..T.......C....GT.....G.....T...N......N.T..C..C..N..T..C...A..C..CA..... 779 Query_14 2068 CTTTCATCAGCGTCATTGAAGAAAGATGATGTATTGACTGTGACATTTGATCTGGAAAATACAGGTAAATATAAGGGGACGGAAGTTGCT 2157 0 1822 ..GG.G..T.ATGT.C....A...........GC....A........C..............C..G......G.A..A........A... 1911 1 780 .. 781 Query_14 2158 CAATTGTATATACAAGATAAGGTTGGTTCTGTAACTCGTCCGGTGAAAGAACTGAAACGTTTTACTCGGGTAACCTTGAAACCGGGCGAG 2247 0 1912 .....................A.......C..G..........................C..C.....T..G..A.....G.....T... 2001 0 1926 ............ 1915 Query_14 2248 AAAAAGAATGTTTCGTTTGAACTACCCGTTAGTGAACTTGCATTTTGGAACATCGATATGGTGAAAGTTGTGGAACCCGGAGACTTTGGA 2337 0 2002 .....A.GC..............C..T..........................A.......CT..........................G 2091 Query_14 2338 CTTTGGGTGGCAACAGACAGCCAATCGGGAGAAGAAGTTTTCTTTAAGGTGGTAGATTAACATTGGAAGTGGATAACGGATCCGAATTCG 2427 0 2092 ..............G..T..T..G..C................. 2135 Query_14 2428 AGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAG 2512 2 718 ........... 708 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11294456 Query= CMR200130.106 Length=2865 Score E Sequences producing significant alignments: (Bits) Value 0_wt 22.9 1.6 2_07E_E2_APC115044.117_E08.seq 21.1 5.5 Query_15 354 TGGCGAAGGGTGTGTAGCTTTCCTTCAGGGAGGAATAGGATGGTATAGCAAATCATTTCCCACAACAATCAGCGCAAATCAGAAATGCTA 443 0 393 ..C........... 406 0 903 ........... 893 2 341 ........... 351 Query_15 444 TATAGTGTTCGATGGAGTATATAATAATTCTGAGTATTGGATAAATGGCAAAAAACTTGGATATCATCTTTCGGGATATGCTCCTTTTTA 533 0 684 ..... 688 2 560 ..... 556 Query_15 534 TTTTGATGTCACAGACTATCTCAATCCCAATGAGGATAACCGCATGACTGTAAGGGTCGACCACAGCCATTATGCCGACAGCAGATGGTA 623 0 689 ...... 694 2 555 ...... 550 Query_15 624 CACCGGTTCAGGTATATACAGGGATGTGAAAATGATTGTAACCGACAGACTGCATATTCCGGTTTGGGGAACATTTGTCACTACTCCCGT 713 Query_15 714 GGTTACTGATAAATATGCTAAAGTAAACAACCAAATTACCGTGCGCAACAGTTACTCTGAACCCAGAACAGCTGTTGTTGAGATAGTGTA 803 Query_15 804 TAAAGATAATAAAGGCAATATCGCAGCCTTTGAGGTCTTCAGTATAAAACTGAATGCTGGTGAGGAGAAAATTATCGACATCGTATCGGA 893 0 30 ............ 41 Query_15 894 GATAAAACAGCCGGATTTGTGGAGCGTCGAGATACCAGTCCTCTATACAGCCGAGACCCGTATTAAGAATGGCGATGAAGTCATTTCTGA 983 Query_15 984 AAACACTGTCAGGTTCGGTATACGAACATTCCACTTTGATGCAGACAAAGGTTTCTTCCTTAACGGAAAAAATATGAAGATAAAAGGAGT 1073 Query_15 1074 ATGCCTGCATCATGATGCCGGTATAGTTGGCACAGCAATGATACGCGATGTGTGGTACCGACGTCTGAAAACCCTTAAGGAAGGAGGATG 1163 Query_15 1164 TAACGCCATCCGCCTTTCGCACAATCCGGGAGCGGATGAGTTTCTGTCTTTGTGCGATGAGATAGGTCTTCTGGTCCAGGAAGAGTTCTT 1253 Query_15 1254 CGATGAGTGGGATTATCCCAAAGATAAAAGGCTCAATATGAAGGAAACGGTAGAAG 1309 0 1889 ..........-.....A..T.. 1909 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12462848 Query= CMR200130.108 Length=2865 Score E Sequences producing significant alignments: (Bits) Value 0_wt 22.9 1.6 2_07E_E2_APC115044.117_E08.seq 21.1 5.5 Query_16 354 TGGCGAAGGGTGTGTAGCTTTCCTTCAGGGAGGAATAGGATGGTATAGCAAATCATTTCCCACAACAATCAGCGCAAATCAGAAATGCTA 443 0 393 ..C........... 406 0 903 ........... 893 2 341 ........... 351 Query_16 444 TATAGTGTTCGATGGAGTATATAATAATTCTGAGTATTGGATAAATGGCAAAAAACTTGGATATCATCTTTCGGGATATGCTCCTTTTTA 533 0 684 ..... 688 2 560 ..... 556 Query_16 534 TTTTGATGTCACAGACTATCTCAATCCCAATGAGGATAACCGCATGACTGTAAGGGTCGACCACAGCCATTATGCCGACAGCAGATGGTA 623 0 689 ...... 694 2 555 ...... 550 Query_16 624 CACCGGTTCAGGTATATACAGGGATGTGAAAATGATTGTAACCGACAGACTGCATATTCCGGTTTGGGGAACATTTGTCACTACTCCCGT 713 Query_16 714 GGTTACTGATAAATATGCTAAAGTAAACAACCAAATTACCGTGCGCAACAGTTACTCTGAACCCAGAACAGCTGTTGTTGAGATAGTGTA 803 Query_16 804 TAAAGATAATAAAGGCAATATCGCAGCCTTTGAGGTCTTCAGTATAAAACTGAATGCTGGTGAGGAGAAAATTATCGACATCGTATCGGA 893 0 30 ............ 41 Query_16 894 GATAAAACAGCCGGATTTGTGGAGCGTCGAGATACCAGTCCTCTATACAGCCGAGACCCGTATTAAGAATGGCGATGAAGTCATTTCTGA 983 Query_16 984 AAACACTGTCAGGTTCGGTATACGAACATTCCACTTTGATGCAGACAAAGGTTTCTTCCTTAACGGAAAAAATATGAAGATAAAAGGAGT 1073 Query_16 1074 ATGCCTGCATCATGATGCCGGTATAGTTGGCACAGCAATGATACGCGATGTGTGGTACCGACGTCTGAAAACCCTTAAGGAAGGAGGATG 1163 Query_16 1164 TAACGCCATCCGCCTTTCGCACAATCCGGGAGCGGATGAGTTTCTGTCTTTGTGCGATGAGATAGGTCTTCTGGTCCAGGAAGAGTTCTT 1253 Query_16 1254 CGATGAGTGGGATTATCCCAAAGATAAAAGGCTCAATATGAAGGAAACGGTAGAAG 1309 0 1889 ..........-.....A..T.. 1909 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12462848 Query= CMR200135.107 Length=2778 Score E Sequences producing significant alignments: (Bits) Value 2_07E_E2_APC115044.117_E08.seq 24.7 0.43 0_wt 24.7 0.43 1_02E_E2_APC115044.117_E06.seq 21.1 5.3 Query_17 273 TTCATTTAATGATTCTCAATGGGAATCTGTCTGTATTCCACACGACTGGGCCATTTATGGACCGTTTGACCGTAATAATGATTTACAAAA 362 2 602 ............. 590 0 642 ............. 654 Query_17 363 TGTAGCCATTACTCAGAACTTGGAGAAACAGGCATCTGTCAAGACCGGACGTACCGGAGGACTTCCTTATGTGGGAGTAGGATGGTATCG 452 Query_17 453 CACCCGTTTCGATGCAGACCCTGACAAAAAGACAACACTGGTTTTTGATGGAGCCATGAGTGAAGCCCGCGTGTATGTCAATGGAAAAGA 542 Query_17 543 AGCCTGCTTCTGGCCTTTCGGTTACAATTCCTTCCATTGTGACATTACTGAGCTTCTGCACAAAGAAGGAAAAGACAATGTATTGGCTGT 632 Query_17 633 ACGTCTGGAAAACCGTCCTCAATCTTCCCGCTGGTATCCGGGAGCCGGACTTTACCGGAATGTCCATCTGATTACTGCAGAAAAAATACA 722 Query_17 723 TGTACCTGTATGGGGAACACAGGTTACCACCCCACACGTAGCTAATGACTATGCTTCTGTTTGCCTTCGTACCTCTTTACAGAATGTGGG 812 Query_17 813 AAAAGAAGAAATTACCATAGAAACAGAAATACTGGACCCGAACGGGAAAAAAGTTTCTTTCAAGAAGAACAGCGGACGCATCAATCACGG 902 Query_17 903 GCAACCGTTTACACAAAATTTCATTGTGGAAAACCCGCAATTGTGGTCACCTGAAACACCGTTCTTATATCAGGCCGTATCTAAAATCTA 992 Query_17 993 TGCCAACGGAAAACTTACAGATACTTATACCACCCGCTTTGGTATCCGTTCCATCGAATTTGTAGCCGACAAGGGCTTTTTCCTGAACGG 1082 2 619 ............. 631 0 625 ............. 613 Query_17 1083 CCAGCACCGTAAATTCCAGGGGGTATGCAACCACCACGACTTAGGTCCTTTAGGAGCTGCCATCAACGTATCGGCTCTACGCCACCAGCT 1172 Query_17 1173 TACATTATTAAAAGACATGGGCTGCGATGCCATTCGTACCGCACACAACATGCCGGCACCCGAGCTTGTCAGACTCTGCGATGAAATGGG 1262 Query_17 1263 ATTCATGATGATGATTGAGCCTTTCGATGAATGGGACATTGCCAAGTGTGAAAACGGATACCACCGCTATTTCAACGAATGGGCCGAAAA 1352 Query_17 1353 AGACATGGTAAACATGCTACGGCAATACCGGAATAATCCCTGTGTGGTGATGTGGAGTATCGGTAATGAAGTACCCACCCAATGCAGCAG 1442 Query_17 1443 TGAAGGATACAAAGTAGCCAAGTTCCTGCAAGACATTTGCCATCGGGAAGACCCTACCCGTCCGGTTACCTGCGGCATGGACCAGGTTAG 1532 Query_17 1533 TTGTGTACTCGACAACGGATTTGCGGCCATGCTCGACATTCCGGGATTCAATGCTCGCGCACACCGCTATGAAGAAGCTTACCAACGCCT 1622 Query_17 1623 GCCTCAAAATCTTGTATTAGGCTCAGAAACCTCTTCTACCGTCAGTTCACGCGGTGTATACAAATTCCCGGCAGAGCGTAAAGCCGATGC 1712 Query_17 1713 AAAATACGAAGACCATCAGTCTTCTTCTTACGACTTGGAATACTGCTCCTGGTCTAACATTCCCGATATAGACTTTGCTCTGGCTGATGA 1802 Query_17 1803 CCACCAATGGACTTTGGGGCAGTTTGTCTGGACAGGTTTTGATTATCTGGGTGAACCCAGTCCATACGATACGGATGCATGGCCCAACCA 1892 Query_17 1893 CAGCTCTATGTTCGGTATTATCGACCTGGCTTCCTTACCCAAAGACCGGTACTATTTATACCGCAGCATATGGAACAAGCAAGCTGAAAC 1982 Query_17 1983 ACTTCATATTCTTCCTCATTGGAACTGGGAGGGCAGAGAAGGAAAAGAAGTACCTGTATTCGTCTATACCAACTATCCGACAGCCGAACT 2072 Query_17 2073 TTTCATCAACGGAAAAAGTTATGGGAAACAGACGAAGAACAACCAAAGCGTAGAGAACCGTTACCGCCTGATGTGGCACAACGCCATTTA 2162 Query_17 2163 CGAACCGGGAGAAGTAAAAGTCGTGGCATACGATGAACACGGTACGGCTAAAGCAGAAAAGATAATCCGCACGGCAGGCAAACCTCACCA 2252 0 1287 ........... 1297 1 240 ........... 250 Query_17 2253 TATTGAATTGGTTTCTTCACGCCAGTCGCTCACAGCCGATGGAAAAGATTTGGCTTACGTAACCGTACGTGTTGTGGACAAAGACGGAAA 2342 Query_17 2343 TCTCTGCCCCACAGATATGCGCTTGGTGAAATTTAAAGTAAAAGGAGCTGGAAGCTACAAAGCCTCAGCCAATGGAGATCCAACTTGTCT 2432 Query_17 2433 GGATTTGTTCCACCTGCCTCAGATGCACGCCTTCAACGGCATGCTGACTGCAATTGTGCAATCAGGAAAAGAAGCAGGTACCCTTGAGTT 2522 Query_17 2523 ACAAGTCACCGCAAAAGGGCTGAAATCAGGAAAGATACAAATCGAAGTAAAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTC 2612 Query_17 2613 CGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAG 2692 2 718 ........... 708 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12082136 Query= CMR200148.108 Length=2607 Score E Sequences producing significant alignments: (Bits) Value 0_wt 62.6 2e-12 2_07E_E2_APC115044.117_E08.seq 33.7 8e-04 1_02E_E2_APC115044.117_E06.seq 22.9 1.4 Query_18 403 CAGCGTATGGCGGTGGAGGAAACTCGTCTGGGAATTCCTTT---GATAGTGGGTGCCGATGTGATTCACGGGTACGAAACAATCTTCCCG 489 0 160 .....CG....AA....A...T.C...T....T........ATT.....C.C...---....C..........TTA.......T.....T 246 Query_18 490 ATTCCGTTGGCCCTGTCTTGTAGCTGGG----ATACGGCGGCGGTGACACGTATGGCACGTATTTCTGCCACGGAAGCCAGTGCCGATGG 575 0 247 .....C....----.A.AA.CG...TC.TTCA..C..CA..TA.C..A.GACGGT.....G..AG.A..T.TT.....TTCGT.T.TA.. 332 Query_18 576 AATCAGCTGGACCTTCAGTCCGATGGTAGACATCTGTCGGGATGCCCGCTGGGGACGTATTGCAGAAGGAAGTGGAGAGGACCCGTACCT 665 0 333 T...C.G.....T..TGCG..A...A.T..T..TGCC..C...C.T.................C.....GT....T..A..TA....... 422 Query_18 666 CGGGGCGTTGATGGCTGGAGCCTATGTGCGCGGTTATCAGGGTGACGGCATGAAGCAGAACAATGAAATCATGGCCTGTGTGAAGCACTT 755 Query_18 756 TGCGCTGTATGGAGCTTCGGAATCGGGACGTGACTACAATTCGGTGGATATGAGTCGAAACCTGATGTATAATGTGTACCTGGCTCCTTA 845 Query_18 846 TAAAGGGGCGGTGGAAGCCGGAGTGGGTTCGGTGATGAGCTCGTTCAATACCATCAACGGGGTACCTGCTACAGCTGACAAATGGCTGCT 935 Query_18 936 GACGGATTTGCTCCGCAATGAGTGGGGGTTCACGGGGTTTGTGGTGACCGCTTACAATTCGATTGGTGAGATGAAGACTCATGGGGTGGC 1025 Query_18 1026 CGACTTGAAGGAGGCTTCTGCACGGGCGTTGAATGCAGGAACGGACATGGATATGGTGGCACATGGTTTCTTGCATACGCTGGAAGCTTC 1115 0 815 ...........GTA..T.....A...... 843 2 429 ...........GTA..T.....A...... 401 Query_18 1116 ATTGAAGGAGAAGGCCGTGACGCAGGAGCGGATTGACGAGGCTTGTCGTCGGGTATTGGAAGCCAAGTATAAGTTAGGATTGTTTGAAAA 1205 0 940 ......CGT..............T.C 965 2 304 ......CGT..............T.C 279 Query_18 1206 TCCTTATAAGTATTGTGATACGCTTCGGGGACGCAAGGAATTGTTTACGGAGGCGAATCGTAAAGCGGCACGTGAGATTGCGGCTGAAAC 1295 0 966 A...... 972 0 1041 ....T. 1046 2 278 A...... 272 2 203 ....T. 198 Query_18 1296 GTTTGTGCTGTTGAAGAACGA-GGGTAAGTTGTTGCCTTTGCAGAAAAAAGGACGCATTGCATTGATTGGGCCGATGGCTGATGCGCAGA 1384 0 1047 .GC.A.T...........T..TAA.G...-.......G..A... 1089 2 197 .GC.A.T...........T..TAA.G...-.......G..A... 155 Query_18 1385 ACAATATGTGCGGCACGTGGAACATGGATTGTCAGACAGACCGTCATGTGACGATGTACGAAGCTTTCCGTCGTGCGGTAGGTGATAAGG 1474 Query_18 1475 CTACGGTTTCTTATGCCAAGGGAAGTAATGTGTATTATAGTGAGCATATTGAGAAAGGGGCGGTCGAACCTCGTCCGCTGACACGTGGCG 1564 Query_18 1565 ATGACCGTCAGTTGCGGGCTGAGGCTTTGCGCGTGGCGGCTTCTGCCGATGTGATTGTGGCCGCATTAGGTGAGAGTGCTGAGATGAGCG 1654 Query_18 1655 GAGAGTCTTCTTCTCGTACAGATATTCAGATTCCGGATGCGCAGAAAGATTTGTTGAAGGCATTGATAGCTACCGGAAAGCCGGTGGTAC 1744 Query_18 1745 TGGCTTTGTTTACCGGTCGTCCGCTGGATTTATGCTGGGAGTCTGAGCATGTTCCGGCTATCCTGAACGTGTGGTTTGCCGGCAGTGAAG 1834 Query_18 1835 CGGGTGATGCCATTGCCGATGTGATGTTTGGAGAAGTATCTCCTTCGGGTAAGCTGACTACGAGTTTCCCACGTGCGGTGGGACAGTTGC 1924 Query_18 1925 CGCTTTATTATAATCACCTGAATACGGGTCGTCCGGATACGGATGACACTACTTTCAATCGTTATGGCAGCAATTACATCGACCAGAGTA 2014 0 552 ........... 542 0 1463 ........... 1473 2 694 ........... 704 1 416 ........... 426 Query_18 2015 ATGAACCGCTTTATCCTTTTGGCTATGGTTTGAGTTATACCACTTTCCGTTACGGTAATTTGCAGTTGAGTGCGGAGCGTATGGCCAAGG 2104 Query_18 2105 GTGGGCAGTTGAAGGTAACCGTGCCTGTAACCAATTCCGGCGAGTGTGACGGAGTAGAGATTGTGCAGTTGTATCTTCACGATGTGTATG 2194 Query_18 2195 CAGAAATCTCCCGTCCGGTGAAGGAGCTGAAA-GCTTTCCGCCGTGTGGCCCTTAAAAAGGGAGAGACACAGAATGTAGAGTTTGTACTC 2283 0 1948 ...........A..A......C.... 1973 Query_18 2284 GATGAGGATGATTTGAAGTATTATAATTCTCGTCTGGAATATGGATATGAACCGGGAGAGTTTGAAGTGATGGTGGGTCCGGACAGCCGG 2373 0 1240 ............ 1251 1 193 ............ 204 Query_18 2374 AATGTGCAGCACGCGACTTTTGTGGCTGAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCA 2463 Query_18 2464 CTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAG 2521 2 718 ........... 708 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11333840 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:35 AM Number of letters in database: 4,427 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115045.110 (APC115045.26-772.P(773).K213A.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVSGEIVTGQAQNSDVAKKIEQGLVGGLLNLKGVEKIRDVQKLAIEKSRLGIPLIFGMDVVHGYETIFPIPLGLSCSWDMEAIRKSARVAAIEASADGISWTFSPMVDISRDPRWGRVSEGNGEDPFLGGAIAKAMVSGYQGIDLNNQLKRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEYMYPYQAAVDAGVGSVMASFNEIDGIPATANKWLMTDVLRKQWGFDGFVVTDFTGISEMIAHGIGDLQTVSARALNAGVDMDMVSEGFTGTIKKSIDEGKISMETLDKACRRILEAKYKLGLFDNPYKYCDLKRPKRDIFTKEHRDAARKIAGESFVLLKNDKSGSSANPTLPLKKEGTVAVIGPLANTRSNMPGTWSVAARLNDYPSVYEGLKEMMKGKVNITYAKGSNLISDAAYEERATMFGRSLNRDNRTDKEMLDEALKVAANADVIIAALGESSEMSGESSSRTNLALPDVQRTLLEALLKTGKPVVLTLFTGRPLTLTWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLLEGKWFEKFRSNYLDVDNDPLYPFGYGLSYTNFQYSDITLSAPTMGQDGSVTAMVTVTNTGKYDGAEVVQLYIRDLVGSITRPVKELKGFDKIFLKAGESKTVSFKITPELLRFYDYELNYVAEPGDFDIMIGGNSQSVKTTHLSL
AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTTTCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAAGGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCATGGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATCGAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAACGGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAACGATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAACCGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGCATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGTATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGTGAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAAGCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGCGACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAAAAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGACTATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCAGCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,860 total letters Query= APC115045.110 Length=2646 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4028 0.0 1_02F_F2_APC115045.110_F06.seq 1427 0.0 2_07F_F2_APC115045.110_F08.seq 1409 0.0 K S P Q D M D R F I D A L M K K M T V E E K I G Q L N L P V Query_1 202 AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTT 291 0 1 .......................................................................................... 90 S G E I V T G Q A Q N S D V A K K I E Q G L V G G L L N L K Query_1 292 TCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAA 381 0 91 .......................................................................................... 180 G V E K I R D V Q K L A I E K S R L G I P L I F G M D V V H Query_1 382 GGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCAT 471 0 181 .......................................................................................... 270 G Y E T I F P I P L G L S C S W D M E A I R K S A R V A A I Query_1 472 GGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATC 561 0 271 .......................................................................................... 360 E A S A D G I S W T F S P M V D I S R D P R W G R V S E Query_1 562 GAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGT-GAT-CCGCGCT--GGGGACGCG--TCAGCGAG 645 0 361 .........................................................-...-.......--.........--........ 444 2 907 ......T...T......NTG.......GNGT.......A 869 G N G E D P F L G G A I A K A M V S G Y Q G I D L Query_1 646 GG--TAAC-GGC-GAAGACCCA--TTCTTGGGT--GGAGCCATCG-CTAAA-GCAATGG-TATCGGG-TTAT-CAGGGTATCGA-CCTCA 721 0 445 ..--....-...-.........--.........--..........-.....-.......-.......-....-...........-..... 520 1 1 ...--...C.--...-...N--.......-.......-....-...........-....G 49 2 868 ..GT....C...N........CNT........GTG..........G.....A.......G.......G....T........N..A...NG 779 N N Q L K R N D E I M A C V A H F A L Y G A G E A G R D Y N Query_1 722 ACAA-CCAACTGAAGCGCAACGATGAAATTATGGCATGTGTAGCTCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAAT 810 0 521 ....-.....................................AAG............................................. 609 1 50 ....-..................................................................................... 138 2 778 ....A......N.............................................................................. 689 T V D M S R N R M F N E Y M Y P Y Q A A V D A G V G S V M A Query_1 811 ACCGTAGATATGAGTCGTAACCGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCG 900 0 610 .......................................................................................... 699 1 139 .......................................................................................... 228 2 688 ....C..................................................................................... 599 S F N E I D G I P A T A N K W L M T D V L R K Q W G F D G F Query_1 901 TCTTTCAACGAAATAGACGGCATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTT 990 0 700 .......................................................................................... 789 1 229 .......................................................................................... 318 2 598 .......................................................................................... 509 V V T D F T G I S E M I A H G I G D L Q T V S A R A L N A G Query_1 991 GTGGTGACAGACTTTACCGGTATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGC 1080 0 790 .......................................................................................... 879 1 319 .......................................................................................... 408 2 508 .......................................................................................... 419 V D M D M V S E G F T G T I K K S I D E G K I S M E T L D K Query_1 1081 GTGGATATGGACATGGTAAGTGAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAA 1170 0 880 .......................................................................................... 969 1 409 .......................................................................................... 498 2 418 .......................................................................................... 329 A C R R I L E A K Y K L G L F D N P Y K Y C D L K R P K R D Query_1 1171 GCCTGTCGCCGCATCCTTGAAGCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGAC 1260 0 970 .......................................................................................... 1059 1 499 .......................................................................................... 588 2 328 .......................................................................................... 239 I F T K E H R D A A R K I A G E S F V L L K N D K S G S S A Query_1 1261 ATCTTCACCAAGGAACATCGCGACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCA 1350 0 1060 .......................................................................................... 1149 1 589 .......................................................................................... 678 2 238 .......................................................................................... 149 N P T L P L K K E G T V A V I G P L A N T R S N M P G T W Query_1 1351 AACCCAACACTTCCTTTGAAAAAAGAAGGTACGGTGGCTGTCATCGG-CCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCT-GGA 1438 0 1150 ...............................................-......................................-... 1237 1 679 ........................N......................C.............N.....N..................G... 768 2 148 ...............................................-......................................-... 61 S V A A R L N D Y P S V Y E G L K E M M K G K V N I T Query_1 1439 GTGTAGCCGCACGCCTCAACGACTATCCTT-CTGTGTACGAA-GGATTG-AAAGAGA-TGA-TGAAAGGCAA--GGTAAACA-TCAC-TT 1519 0 1238 ..............................-...........-......-.......-...-..........--........-....-.. 1318 1 769 N.................N...........N...........G......N..N..N.T...T..........NG........T..N.T.. 858 2 60 ..............................N........N..-..-N..-...T...-.. 5 Y A K G S N L I S D A A Y E E R A T M F G R S L N Query_1 1520 ATGCC--AAAGGTAGT-AACCTCAT-CAGT-GATGCAG--CCTACG-AAGAACG-TGCCACAA--TGTTC-GGCCG--TTCATT--AAAT 1593 0 1319 .....--.........-........-....-.......--......-.......-........--.....-.....--......--.... 1392 1 859 .....CA..G....NNA........C....G....GN.NC......A..N..NNT....N...AT.....G....NGT......TA.... 948 R D N R T D K E M L D E A L K V A A N A D V I I A A L G E S Query_1 1594 CGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCA 1683 0 1393 .......................................................................................... 1482 1 949 .. 950 S E M S G E S S S R T N L A L P D V Q R T L L E A L L K T G Query_1 1684 TCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGA 1773 0 1483 .......................................................................................... 1572 K P V V L T L F T G R P L T L T W E Q E H V P A I L N V W F Query_1 1774 AAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTC 1863 0 1573 .......................................................................................... 1662 G G S E A A Y A I G D V L F G D V N P S G K L T M T F P K N Query_1 1864 GGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAAC 1953 0 1663 .......................................................................................... 1752 V G Q I P L F Y N H K N T G R P L L E G K W F E K F R S N Y Query_1 1954 GTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTAC 2043 0 1753 .......................................................................................... 1842 L D V D N D P L Y P F G Y G L S Y T N F Q Y S D I T L S A P Query_1 2044 CTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCG 2133 0 1843 .......................................................................................... 1932 T M G Q D G S V T A M V T V T N T G K Y D G A E V V Q L Y I Query_1 2134 ACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATC 2223 0 1933 .......................................................................................... 2022 R D L V G S I T R P V K E L K G F D K I F L K A G E S K T V Query_1 2224 CGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTA 2313 0 2023 .......................................................................................... 2112 S F K I T P E L L R F Y D Y E L N Y V A E P G D F D I M I G Query_1 2314 TCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGG 2403 0 2113 .......................................................................................... 2202 G N S Q S V K T T H L S L Query_1 2404 GGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG 2442 0 2203 ....................................... 2241 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12642861 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:35 AM Number of letters in database: 4,860 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200122.105 (CMR200122.20-750.P(750).D106A.pMCSG68)
TQVANKGSDAATEKKVESLLSRMTLEEKIGQMNQITSYGNIEDMSSLIKKGEVGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQIAKDGARVAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKDVLRGEWGFDGLVVTDWASASEMISHGFAADSKEVAMKSVNAGVDMEMVSYTFVKELPALIKEGKVKESTIDEAVRNILRVKYRLGLFDVPYVDEKQPSVMYDPSHLKVAKQAAVESAILLKNDKEVLPLQESLKTIAVVGPMANAPYEQLGTWIFDGEKAHTQTPLNAIKEIVGDKVQVIYEPGLAYSREKNPAGVAKAAAVAARADVILAFVGEEAILSGEAHCLADLNLQGDQSALITALAKTGKPVVTIVMAGRPLTIGQEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKTPVTFPKMVGQIPVYYAHNNTGRPATRNEVLLDDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNVKLSSASLKKDDVLTVTFDLENTGKYKGTEVAQLYIQDKVGSVTRPVKELKRFTRVTLKPGEKKNVSFELPVSELAFWNIDMVKVVEPGDFGLWVATDSQSGEEVFFKVVD
ACACAAGTGGCAAATAAAGGTAGCGATGCGGCAACCGAGAAAAAAGTAGAGTCTCTTTTATCCAGAATGACCCTTGAAGAGAAAATCGGTCAGATGAACCAGATTACCTCTTACGGGAATATTGAGGATATGAGTAGTTTAATTAAGAAAGGTGAAGTCGGGTCTATCCTGAATGAGGTGGATCCGGTACGTATTAATGCGTTGCAACGCGTAGCGATGGAGGAGTCCCGGTTGGGAATCCCTTTGTTGATAGCTCGCGATGTTATTCACGGGTTTAAAACCATTTTTCCCATCCCATTGGGACAAGCGGCTTCGTTCAATCCGCAGATTGCGAAAGACGGTGCACGGGTAGCGGCTATTGAGGCTTCTTCCGTAGGTATCCGTTGGACTTTTGCACCGATGATCGACATTGCCCGTGATCCTCGCTGGGGGCGCATTGCCGAAGGATGTGGTGAAGACACTTACCTGACTTCTGTAATGGGAGCTGCCATGGTAGAAGGTTTTCAGGGAGATTCTTTGAATAGTCCCACTTCCATAGCTGCCTGTCCTAAACATTTTGTGGGCTATGGTGCAGCTGAAGGCGGACGTGACTATAATTCGACATTTATTCCTGAACGTCGCCTGCGTAATGTTTACTTGCCACCGTTTGAAGCGGCAACGAAAGCGGGTGCAGCTACGTTTATGACTTCCTTTAATGATAATGATGGGATACCCTCTACCGGAAATGCTTTCATATTGAAAGATGTGCTTCGTGGCGAGTGGGGATTTGATGGTTTGGTAGTGACAGACTGGGCTTCTGCCAGCGAAATGATAAGTCATGGTTTTGCTGCCGATTCTAAAGAGGTAGCCATGAAATCAGTGAATGCTGGGGTGGATATGGAAATGGTAAGTTATACCTTTGTAAAAGAATTGCCTGCATTGATAAAAGAAGGAAAGGTGAAAGAAAGCACCATTGATGAAGCCGTTCGTAATATATTGCGCGTCAAGTATCGTCTGGGATTGTTTGATGTTCCTTATGTAGATGAAAAGCAACCCTCTGTCATGTATGATCCTTCTCATCTGAAAGTAGCTAAGCAGGCTGCTGTAGAATCGGCTATCCTGTTGAAGAATGATAAAGAAGTACTGCCGTTACAGGAGTCTCTGAAAACCATTGCTGTGGTAGGACCTATGGCCAATGCGCCTTATGAACAATTGGGTACCTGGATCTTTGATGGTGAGAAAGCTCATACTCAGACACCACTGAATGCTATTAAGGAAATAGTTGGCGACAAAGTACAGGTGATTTATGAACCCGGATTAGCTTATAGCCGTGAGAAAAATCCGGCAGGCGTAGCAAAAGCTGCTGCTGTTGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAAGCCATTCTTTCGGGTGAAGCACACTGTCTGGCAGATTTGAATCTTCAGGGTGATCAAAGTGCTTTGATTACGGCTTTGGCTAAGACAGGTAAACCTGTAGTAACCATTGTGATGGCAGGTCGTCCGTTGACTATCGGTCAGGAAGTGGAAGAATCAACAGCTGTTCTTTATTCATTCCATCCGGGTACGATGGGTGGACCGGCATTGGCCGATCTGCTGTGGGGTAAGGCGGTTCCAAGTGGAAAAACACCGGTTACTTTCCCGAAGATGGTAGGACAAATTCCGGTATATTATGCTCATAACAATACCGGGCGGCCGGCTACACGTAATGAGGTGTTGCTGGATGATATTGCTGTTGAGGCTGGACAAACTTCATTGGGATGTACTTCTTTCTATATGGATGCCGGTTTTGATCCTTTATTCCCATTTGGCTATGGCTTGTCGTATACAACGTTCAAGTATAGTAATGTCAAACTTTCATCAGCGTCATTGAAGAAAGATGATGTATTGACTGTGACATTTGATCTGGAAAATACAGGTAAATATAAGGGGACGGAAGTTGCTCAATTGTATATACAAGATAAGGTTGGTTCTGTAACTCGTCCGGTGAAAGAACTGAAACGTTTTACTCGGGTAACCTTGAAACCGGGCGAGAAAAAGAATGTTTCGTTTGAACTACCCGTTAGTGAACTTGCATTTTGGAACATCGATATGGTGAAAGTTGTGGAACCCGGAGACTTTGGACTTTGGGTGGCAACAGACAGCCAATCGGGAGAAGAAGTTTTCTTTAAGGTGGTAGAT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,322 total letters Query= APC113179.120 Length=1128 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1305 0.0 2_08G_G3_APC113179.120T7term.seq 1182 0.0 1_03G_G3_APC113179.120T7sh.seq 1182 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 59 TCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATT-CTTCTGGTGTAGATCTGTGG 147 1 4 .......-....-........N.......................................C.C.TT..N.NN..............N.. 91 S H P Q F E K G T E N L Y F Q S N A V D Q Y K K E I G Query_1 148 TCTCAT--CCGCAGTT-CGAAAA--GGGTA-CCGAG-AACCTGTAC-TTCCAAT-CCAATGCCGTAGATCAGTATAAGAAAGAAATCGGA 228 0 1 ........................... 27 2 1904 .....TC..N.....T......AA.....C.....A.....N...T.......A.... 1847 1 92 ......--........-..CNTT--.....-.....-.........-.......-.................N................. 172 M M N L E K L I Q D R E G I S S K K F P I E D K E Query_1 229 ATGATGAATCTAGAAAAATTGA--TTCAGG-ATC-GTG-AAGGAA-TATCTTCGAAAAA--GTTT-CCT-ATAGAGG--ATAAA-GAATT 305 0 28 ......................--......-...-...-......-.............--....-...-.......--.....-..... 104 2 826 .........AT......T...N...G......A.......NN....AT....N...N....GN.GN.N...N..... 750 1 173 ......................--......-...-...-......-.............--....-...-.......--.....-..... 249 L L F R Y E Q L Y T G A V N D V M R E F C L L E Q A L P Query_1 306 ATTATT-TCGATA-CG-AGCA-GTTGTATACAGGGG-CCGTAAA-CGATGTGATGCGTGAATTTTGCCTTTTGGAGCAAGCCTTACCCGG 389 0 105 ......-......-..-....-..............-.......-............................................. 188 2 749 ......A.....NN..N...NN.....N........N.......A.....N............N.............N...NN.....N. 660 1 250 ..N...-......-..-....-..............-.......-............N............N................... 333 G R I K P L R E Y H S V A G F A F T V K S A P N V K I K G E Query_1 390 TAGGATTAAACCTTTACGCGAATATCATTC-TGTTGCCGGTTTTGCCTTTACTGTCAAAAGTGCACCAAACGTAAAGATAAAGGGTGAGA 478 0 189 ..............................-........................................................... 277 2 659 A............N.N..............C.........N..A.....NCN...................................... 570 1 334 ..............................-......................................................N.... 422 M E Y R T Q M L D E M Q E D H F V V W D T S R D D K A T L W Query_1 479 -TGGAATATCGAACCCAGATGCTGGATGAGATGCAGGAAGACCATTTTGTTGTCTGGGATACAAGTCGGGATGATAAAGCTACTTTGTGG 567 0 278 -......................................................................................... 366 2 569 A......N....N...N.N..N..............N..................................................... 480 2 2144 ............... 2158 1 423 -..............................................N...........N...........................C.. 511 G G V M T A T A K G K K L K A A C I D G G I R D T H Q I L N Query_1 568 GGAGGTGTAATGACGGCAACTGCTAAAGGGAAGAAACTGAAAGCCGCTTGTATAGATGGGGGAATACGGGATACACATCAGATTTTGAAT 657 0 367 .......................................................................................... 456 2 479 .......................................................................................... 390 2 1513 ........... 1523 1 512 ..................................................N..........................C.N.......... 601 A D F P V F Y E Y R I S N G S L G R C L I T H Y Q I P I Query_1 658 GCTGATTT-TCCTGTGTTTTACGAATAT-CGTATATCGAATGGTAGCTT-GGGGCGCTGTTTGATTACTCACTATCAG-ATTCCAATTAA 743 0 457 ........-...................-....................-............................-........... 542 2 389 ........-...................-....................-............................-........... 304 1 602 ........C.......N...........C....................G.........N..................N.N.....N.N. 691 K I G D V T I K P G D I I L G D I D G V L V V P R E I A Query_1 744 AATCGGAGATGTAACGATTAAG-CCGGGTGATATAATA-CTTGGAG-ATATCGAT--GGAGTTTTAGTT-GTACCA-AGGG-AAATAGCT 825 0 543 ......................-...............-.......-........--............-......-....-........ 624 2 303 ......................-...............-.......-........--............-..N...-....-........ 222 1 692 ......................C.....N..C.N....C.......C...N.NGNCG....N..C....G.C....C....A...N.... 781 Y E V L L R S E E I R E N E K K I F S W V Q N G D S Query_1 826 T-ATGAAGTGCTTTTA-CGCTCGG--AGGAGATACGT-GAAAATGA-GAAGAAG-ATTTTCTCTT--GGGTACAA-AACGGA-GACAGTG 904 0 625 .-..............-.......--...........-........-.......-..........--........-......-....... 703 2 221 .-..............-.......--...........-........-.......-..........--........-......-....... 143 1 782 .A.N...........NC.C...N.NA....C.N....N.....N..CN......A.C........TG...N....N......NN..G... 871 V Q E I T A K * H W K W I T D P N S S S V D K L A A A L E Query_1 905 TTCA--GGAAA-TTACAGCAAAATAACATTGGAAGTGGATAA-CGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAG 990 0 704 ....--.....-........... 723 2 142 ....--.....-..............................T............................................... 56 1 872 N...AG.....T....GC..... 894 H H H H H H * D P A A N K A R K E Query_1 991 caccaccaccacca-ccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAG 1042 2 55 .N...........NC............N.......T........N..N..... 3 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4752688 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:35 AM Number of letters in database: 4,322 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200122.108 (CMR200122.20-750.P(750).E489A.pMCSG68)
TQVANKGSDAATEKKVESLLSRMTLEEKIGQMNQITSYGNIEDMSSLIKKGEVGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQIAKDGARVAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKDVLRGEWGFDGLVVTDWASASEMISHGFAADSKEVAMKSVNAGVDMEMVSYTFVKELPALIKEGKVKESTIDEAVRNILRVKYRLGLFDVPYVDEKQPSVMYDPSHLKVAKQAAVESAILLKNDKEVLPLQESLKTIAVVGPMANAPYEQLGTWIFDGEKAHTQTPLNAIKEIVGDKVQVIYEPGLAYSREKNPAGVAKAAAVAARADVILAFVGEEAILSGEAHCLADLNLQGDQSALITALAKTGKPVVTIVMAGRPLTIGQEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKTPVTFPKMVGQIPVYYAHNNTGRPATRNEVLLDDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNVKLSSASLKKDDVLTVTFDLENTGKYKGTEVAQLYIQDKVGSVTRPVKELKRFTRVTLKPGEKKNVSFELPVSELAFWNIDMVKVVEPGDFGLWVATDSQSGEEVFFKVVD
ACACAAGTGGCAAATAAAGGTAGCGATGCGGCAACCGAGAAAAAAGTAGAGTCTCTTTTATCCAGAATGACCCTTGAAGAGAAAATCGGTCAGATGAACCAGATTACCTCTTACGGGAATATTGAGGATATGAGTAGTTTAATTAAGAAAGGTGAAGTCGGGTCTATCCTGAATGAGGTGGATCCGGTACGTATTAATGCGTTGCAACGCGTAGCGATGGAGGAGTCCCGGTTGGGAATCCCTTTGTTGATAGCTCGCGATGTTATTCACGGGTTTAAAACCATTTTTCCCATCCCATTGGGACAAGCGGCTTCGTTCAATCCGCAGATTGCGAAAGACGGTGCACGGGTAGCGGCTATTGAGGCTTCTTCCGTAGGTATCCGTTGGACTTTTGCACCGATGATCGACATTGCCCGTGATCCTCGCTGGGGGCGCATTGCCGAAGGATGTGGTGAAGACACTTACCTGACTTCTGTAATGGGAGCTGCCATGGTAGAAGGTTTTCAGGGAGATTCTTTGAATAGTCCCACTTCCATAGCTGCCTGTCCTAAACATTTTGTGGGCTATGGTGCAGCTGAAGGCGGACGTGACTATAATTCGACATTTATTCCTGAACGTCGCCTGCGTAATGTTTACTTGCCACCGTTTGAAGCGGCAACGAAAGCGGGTGCAGCTACGTTTATGACTTCCTTTAATGATAATGATGGGATACCCTCTACCGGAAATGCTTTCATATTGAAAGATGTGCTTCGTGGCGAGTGGGGATTTGATGGTTTGGTAGTGACAGACTGGGCTTCTGCCAGCGAAATGATAAGTCATGGTTTTGCTGCCGATTCTAAAGAGGTAGCCATGAAATCAGTGAATGCTGGGGTGGATATGGAAATGGTAAGTTATACCTTTGTAAAAGAATTGCCTGCATTGATAAAAGAAGGAAAGGTGAAAGAAAGCACCATTGATGAAGCCGTTCGTAATATATTGCGCGTCAAGTATCGTCTGGGATTGTTTGATGTTCCTTATGTAGATGAAAAGCAACCCTCTGTCATGTATGATCCTTCTCATCTGAAAGTAGCTAAGCAGGCTGCTGTAGAATCGGCTATCCTGTTGAAGAATGATAAAGAAGTACTGCCGTTACAGGAGTCTCTGAAAACCATTGCTGTGGTAGGACCTATGGCCAATGCGCCTTATGAACAATTGGGTACCTGGATCTTTGATGGTGAGAAAGCTCATACTCAGACACCACTGAATGCTATTAAGGAAATAGTTGGCGACAAAGTACAGGTGATTTATGAACCCGGATTAGCTTATAGCCGTGAGAAAAATCCGGCAGGCGTAGCAAAAGCTGCTGCTGTTGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAAGCCATTCTTTCGGGTGAAGCACACTGTCTGGCAGATTTGAATCTTCAGGGTGATCAAAGTGCTTTGATTACGGCTTTGGCTAAGACAGGTAAACCTGTAGTAACCATTGTGATGGCAGGTCGTCCGTTGACTATCGGTCAGGAAGTGGAAGAATCAACAGCTGTTCTTTATTCATTCCATCCGGGTACGATGGGTGGACCGGCATTGGCCGATCTGCTGTGGGGTAAGGCGGTTCCAAGTGGAAAAACACCGGTTACTTTCCCGAAGATGGTAGGACAAATTCCGGTATATTATGCTCATAACAATACCGGGCGGCCGGCTACACGTAATGAGGTGTTGCTGGATGATATTGCTGTTGAGGCTGGACAAACTTCATTGGGATGTACTTCTTTCTATATGGATGCCGGTTTTGATCCTTTATTCCCATTTGGCTATGGCTTGTCGTATACAACGTTCAAGTATAGTAATGTCAAACTTTCATCAGCGTCATTGAAGAAAGATGATGTATTGACTGTGACATTTGATCTGGAAAATACAGGTAAATATAAGGGGACGGAAGTTGCTCAATTGTATATACAAGATAAGGTTGGTTCTGTAACTCGTCCGGTGAAAGAACTGAAACGTTTTACTCGGGTAACCTTGAAACCGGGCGAGAAAAAGAATGTTTCGTTTGAACTACCCGTTAGTGAACTTGCATTTTGGAACATCGATATGGTGAAAGTTGTGGAACCCGGAGACTTTGGACTTTGGGTGGCAACAGACAGCCAATCGGGAGAAGAAGTTTTCTTTAAGGTGGTAGAT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,929 total letters Query= CMR200122.108 Length=2598 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3947 0.0 2_07H_H2_CMR200122.108_H08.seq 1355 0.0 1_02H_H2_CMR200122.108_H06.seq 1000 0.0 T Q V A N K G S D A A T E K K V E S L L S R M T L E E K I G Query_1 202 ACACAAGTGGCAAATAAAGGTAGCGATGCGGCAACCGAGAAAAAAGTAGAGTCTCTTTTATCCAGAATGACCCTTGAAGAGAAAATCGGT 291 0 1 .......................................................................................... 90 Q M N Q I T S Y G N I E D M S S L I K K G E V G S I L N E V Query_1 292 CAGATGAACCAGATTACCTCTTACGGGAATATTGAGGATATGAGTAGTTTAATTAAGAAAGGTGAAGTCGGGTCTATCCTGAATGAGGTG 381 0 91 .......................................................................................... 180 D P V R I N A L Q R V A M E E S R L G I P L L I A R D V I H Query_1 382 GATCCGGTACGTATTAATGCGTTGCAACGCGTAGCGATGGAGGAGTCCCGGTTGGGAATCCCTTTGTTGATAGCTCGCGATGTTATTCAC 471 0 181 .......................................................................................... 270 0 210 ............ 199 G F K T I F P I P L G Q A A S F N P Q I A K D G A R V A A I Query_1 472 GGGTTTAAAACCATTTTTCCCATCCCATTGGGACAAGCGGCTTCGTTCAATCCGCAGATTGCGAAAGACGGTGCACGGGTAGCGGCTATT 561 0 271 .......................................................................................... 360 E A S S V G I R W T F A P M I D I A R D P R W G R I A E G C Query_1 562 GAGGCTTCTTCCGTAGGTATCCGTTGGACTTTTGCACCGATGATCGACATTGCCCGTGATCCTCGCTGGGGGCGCATTGCCGAAGGATGT 651 0 361 .......................................................................................... 450 G E D T Y L T S V M G A A M V E G F Q G D S L N S P T S I A Query_1 652 GGTGAAGACACTTACCTGACTTCTGTAATGGGAGCTGCCATGGTAGAAGGTTTTCAGGGAGATTCTTTGAATAGTCCCACTTCCATAGCT 741 0 451 .......................................................................................... 540 A C P K H F V G Y G A A E G G R D Y N S T F I P E R R L Query_1 742 GCCTGTCCTAAACATTTTGTGGGCTATGGTGCAGCTGAAGGCGGACGTGACTATAATTCGA-CATTTATT--CCTG-AACGTCGCCTG-C 826 0 541 .............................................................-........--....-...........-. 625 2 890 ......NN.....N..TC....N...........G. 855 R N V Y L P P F E A A T K A G A A T F M T S F N D Query_1 827 GT-AATGTTTACTTGCCACCG---TTTGAA-GCGGC--AACGAAA--GCGGGTG-CAGCT-ACGTTT-ATG-ACTTCCTTT-AATGATAA 902 0 626 ..-..................---......-.....--.......--.......-.....-......-...-.........-........ 701 2 854 .NN..............C..CCGT......N....NCA....G..TGN....G.G..AN.G......T...G.........T........ 765 N D G I P S T G N A F I L K D V L R G E W G F D G L V V T D Query_1 903 TGATGGGATACCCTCTA-CCGGAAA-TGCTTTCATATTGAAAGATGTGCTTCGTGGCGAGTGGGGATTTGATGGTTTGGTAGTGACAGAC 990 0 702 .................-.......-................................................................ 789 2 764 ......N.N........N.......G.......N...........N..N......................................... 675 W A S A S E M I S H G F A A D S K E V A M K S V N A G V D M Query_1 991 TGGGCTTCTGCCAGCGAAATGATAAGTCATGGTTTTGCTGCCGATTCTAAAGAGGTAGCCATGAAATCAGTGAATGCTGGGGTGGATATG 1080 0 790 .......................................................................................... 879 2 674 .......................................................................................... 585 E M V S Y T F V K E L P A L I K E G K V K E S T I D E A V R Query_1 1081 GAAATGGTAAGTTATACCTTTGTAAAAGAATTGCCTGCATTGATAAAAGAAGGAAAGGTGAAAGAAAGCACCATTGATGAAGCCGTTCGT 1170 0 880 .......................................................................................... 969 2 584 .............................................N............................................ 495 N I L R V K Y R L G L F D V P Y V D E K Q P S V M Y D P S H Query_1 1171 AATATATTGCGCGTCAAGTATCGTCTGGGATTGTTTGATGTTCCTTATGTAGATGAAAAGCAACCCTCTGTCATGTATGATCCTTCTCAT 1260 0 970 .......................................................................................... 1059 2 494 .......................................................................................... 405 L K V A K Q A A V E S A I L L K N D K E V L P L Q E S L K T Query_1 1261 CTGAAAGTAGCTAAGCAGGCTGCTGTAGAATCGGCTATCCTGTTGAAGAATGATAAAGAAGTACTGCCGTTACAGGAGTCTCTGAAAACC 1350 0 1060 .......................................................................................... 1149 2 404 .......................................................................................... 315 I A V V G P M A N A P Y E Q L G T W I F D G E K A H T Q Query_1 1351 ATTGCTGTGGTAGGACCTATGGCCAATGCGCCTTATGAACAATTG-GGTACC-TGGATCTTTGATGGTGAGAAAGC-TCATACT-CAGAC 1436 0 1150 .............................................-......-.......................-.......-..... 1235 2 314 .............................................-......-.......................-.......-..... 229 1 110 ...N...N......G.......................G.......T..... 161 T P L N A I K E I V G D K V Q V I Y E P G L A Y S R E K N P Query_1 1437 ACCACTGAATGCTATTAAGGAAATAGTTGGCGACAAAGTACAGGTGATTTATGAACCCGGATTAGCTTATAGCCGTGAGAAAAATCCGGC 1526 0 1236 .......................................................................................... 1325 2 228 .......................................................................................... 139 1 162 ..................................N....................................................... 251 A G V A K A A A V A A R A D V I L A F V G E E A I L S G A A Query_1 1527 AGGCGTAGCAAAAGCTGCTGCTGTTGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAAGCCATTCTTTCGGGTGCTGCA-C 1615 0 1326 ...................................................................................AA...-. 1414 0 1363 ............ 1352 2 138 ........................................................................................G. 49 2 101 ............ 112 1 252 ........................................................N..........N....................-. 340 1 289 ............ 278 H C L A D L N L Q G D Q S A L I T A L A K T G K P V V T I V Query_1 1616 ACTGTCTGGCAGATTTGAATCTTCAGGGTGATCAAAGTGCTTTGATTACGGCTTTGGCTAAGACAGGTAAACCTGTAGTAACCATTGTGA 1705 0 1415 .......................................................................................... 1504 2 48 .............-....................... 13 1 341 ....................................................................N..................... 430 M A G R P L T I G Q E V E E S T A V L Y S F H P G T M G G P Query_1 1706 TGGCAGGTCGTCCGTTGACTATCGGTCAGGAAGTGGAAGAATCAACAGCTGTTCTTTATTCATTCCATCCGGGTACGATGGGTGGACCGG 1795 0 1505 .......................................................................................... 1594 1 431 ...................................N......................N..N....N...............N....... 520 A L A D L L W G K A V P S G K T P V T F P K M V G Q I P V Y Query_1 1796 CATTGGCCGATCTGCTGTGGGGTAAGG-CGGTTCCAAGTGGAAAAACACCGGTTACTTTCCCGAAGATGGTAGGACAAATTCCGGTATAT 1884 0 1595 ...........................-.............................................................. 1683 1 521 .................N..N......A.....................................N.....N.................. 610 Y A H N N T G R P A T R N E V L L D D I A V E A G Q T S L Query_1 1885 TATGCTCATAACAATACCGGGCGGCCGGCTACACGTAATGAGGTGTTGCTGGATGATATTGCTGTTGAGGCTGGACAAACTTCATTGGG- 1973 0 1684 .........................................................................................- 1772 1 611 ........N..................N..N............C............N.N.......................NN.....C 700 G C T S F Y M D A G F D P L F P F G Y G L S Y T T F K Y Query_1 1974 -ATGTACTTCTTTCTATATGGATG-CCGGTTTT-GATCCTTTATTCCC-ATTTGG-CTATGGCTT-GTCG-TATACAACGTTCAAGTATA 2056 0 1773 -.......................-........-..............-......-.........-....-................... 1855 1 701 CNN.N.......N....N..N..NC........N......N.N.....N..N...C......N..N....C.....N......N.N.... 790 S N V K L S S A S L K K D D V L T V T F D L E N T G K Y K G Query_1 2057 G-TAATGTCAAACTTTCATCAGCGTCATTGAAGAAAGATGATGTATTGACTGTGACATTTGATCTGGAAAATACAGGTAAATATAAGGGG 2145 0 1856 .-........................................................................................ 1944 1 791 .C.... 796 T E V A Q L Y I Q D K V G S V T R P V K E L K R F T R V T L Query_1 2146 ACGGAAGTTGCTCAATTGTATATACAAGATAAGGTTGGTTCTGTAACTCGTCCGGTGAAAGAACTGAAACGTTTTACTCGGGTAACCTTG 2235 0 1945 .......................................................................................... 2034 0 1971 ............ 1960 K P G E K K N V S F E L P V S E L A F W N I D M V K V V E P Query_1 2236 AAACCGGGCGAGAAAAAGAATGTTTCGTTTGAACTACCCGTTAGTGAACTTGCATTTTGGAACATCGATATGGTGAAAGTTGTGGAACCC 2325 0 2035 .......................................................................................... 2124 G D F G L W V A T D S Q S G E E V F F K V V D Query_1 2326 GGAGACTTTGGACTTTGGGTGGCAACAGACAGCCAATCGGGAGAAGAAGTTTTCTTTAAGGTGGTAGAT 2394 0 2125 ..................................................................... 2193 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12590118 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 20, 2017 8:35 AM Number of letters in database: 4,929 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200122.112 (CMR200122.20-750.P(750).R588A.pMCSG68)
TQVANKGSDAATEKKVESLLSRMTLEEKIGQMNQITSYGNIEDMSSLIKKGEVGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQIAKDGARVAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKDVLRGEWGFDGLVVTDWASASEMISHGFAADSKEVAMKSVNAGVDMEMVSYTFVKELPALIKEGKVKESTIDEAVRNILRVKYRLGLFDVPYVDEKQPSVMYDPSHLKVAKQAAVESAILLKNDKEVLPLQESLKTIAVVGPMANAPYEQLGTWIFDGEKAHTQTPLNAIKEIVGDKVQVIYEPGLAYSREKNPAGVAKAAAVAARADVILAFVGEEAILSGEAHCLADLNLQGDQSALITALAKTGKPVVTIVMAGRPLTIGQEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKTPVTFPKMVGQIPVYYAHNNTGRPATRNEVLLDDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNVKLSSASLKKDDVLTVTFDLENTGKYKGTEVAQLYIQDKVGSVTRPVKELKRFTRVTLKPGEKKNVSFELPVSELAFWNIDMVKVVEPGDFGLWVATDSQSGEEVFFKVVD
ACACAAGTGGCAAATAAAGGTAGCGATGCGGCAACCGAGAAAAAAGTAGAGTCTCTTTTATCCAGAATGACCCTTGAAGAGAAAATCGGTCAGATGAACCAGATTACCTCTTACGGGAATATTGAGGATATGAGTAGTTTAATTAAGAAAGGTGAAGTCGGGTCTATCCTGAATGAGGTGGATCCGGTACGTATTAATGCGTTGCAACGCGTAGCGATGGAGGAGTCCCGGTTGGGAATCCCTTTGTTGATAGCTCGCGATGTTATTCACGGGTTTAAAACCATTTTTCCCATCCCATTGGGACAAGCGGCTTCGTTCAATCCGCAGATTGCGAAAGACGGTGCACGGGTAGCGGCTATTGAGGCTTCTTCCGTAGGTATCCGTTGGACTTTTGCACCGATGATCGACATTGCCCGTGATCCTCGCTGGGGGCGCATTGCCGAAGGATGTGGTGAAGACACTTACCTGACTTCTGTAATGGGAGCTGCCATGGTAGAAGGTTTTCAGGGAGATTCTTTGAATAGTCCCACTTCCATAGCTGCCTGTCCTAAACATTTTGTGGGCTATGGTGCAGCTGAAGGCGGACGTGACTATAATTCGACATTTATTCCTGAACGTCGCCTGCGTAATGTTTACTTGCCACCGTTTGAAGCGGCAACGAAAGCGGGTGCAGCTACGTTTATGACTTCCTTTAATGATAATGATGGGATACCCTCTACCGGAAATGCTTTCATATTGAAAGATGTGCTTCGTGGCGAGTGGGGATTTGATGGTTTGGTAGTGACAGACTGGGCTTCTGCCAGCGAAATGATAAGTCATGGTTTTGCTGCCGATTCTAAAGAGGTAGCCATGAAATCAGTGAATGCTGGGGTGGATATGGAAATGGTAAGTTATACCTTTGTAAAAGAATTGCCTGCATTGATAAAAGAAGGAAAGGTGAAAGAAAGCACCATTGATGAAGCCGTTCGTAATATATTGCGCGTCAAGTATCGTCTGGGATTGTTTGATGTTCCTTATGTAGATGAAAAGCAACCCTCTGTCATGTATGATCCTTCTCATCTGAAAGTAGCTAAGCAGGCTGCTGTAGAATCGGCTATCCTGTTGAAGAATGATAAAGAAGTACTGCCGTTACAGGAGTCTCTGAAAACCATTGCTGTGGTAGGACCTATGGCCAATGCGCCTTATGAACAATTGGGTACCTGGATCTTTGATGGTGAGAAAGCTCATACTCAGACACCACTGAATGCTATTAAGGAAATAGTTGGCGACAAAGTACAGGTGATTTATGAACCCGGATTAGCTTATAGCCGTGAGAAAAATCCGGCAGGCGTAGCAAAAGCTGCTGCTGTTGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAAGCCATTCTTTCGGGTGAAGCACACTGTCTGGCAGATTTGAATCTTCAGGGTGATCAAAGTGCTTTGATTACGGCTTTGGCTAAGACAGGTAAACCTGTAGTAACCATTGTGATGGCAGGTCGTCCGTTGACTATCGGTCAGGAAGTGGAAGAATCAACAGCTGTTCTTTATTCATTCCATCCGGGTACGATGGGTGGACCGGCATTGGCCGATCTGCTGTGGGGTAAGGCGGTTCCAAGTGGAAAAACACCGGTTACTTTCCCGAAGATGGTAGGACAAATTCCGGTATATTATGCTCATAACAATACCGGGCGGCCGGCTACACGTAATGAGGTGTTGCTGGATGATATTGCTGTTGAGGCTGGACAAACTTCATTGGGATGTACTTCTTTCTATATGGATGCCGGTTTTGATCCTTTATTCCCATTTGGCTATGGCTTGTCGTATACAACGTTCAAGTATAGTAATGTCAAACTTTCATCAGCGTCATTGAAGAAAGATGATGTATTGACTGTGACATTTGATCTGGAAAATACAGGTAAATATAAGGGGACGGAAGTTGCTCAATTGTATATACAAGATAAGGTTGGTTCTGTAACTCGTCCGGTGAAAGAACTGAAACGTTTTACTCGGGTAACCTTGAAACCGGGCGAGAAAAAGAATGTTTCGTTTGAACTACCCGTTAGTGAACTTGCATTTTGGAACATCGATATGGTGAAAGTTGTGGAACCCGGAGACTTTGGACTTTGGGTGGCAACAGACAGCCAATCGGGAGAAGAAGTTTTCTTTAAGGTGGTAGAT
Clone ID: CMR200122.115 (CMR200122.20-750.P(750).E489Q.pMCSG68)
TQVANKGSDAATEKKVESLLSRMTLEEKIGQMNQITSYGNIEDMSSLIKKGEVGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQIAKDGARVAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKDVLRGEWGFDGLVVTDWASASEMISHGFAADSKEVAMKSVNAGVDMEMVSYTFVKELPALIKEGKVKESTIDEAVRNILRVKYRLGLFDVPYVDEKQPSVMYDPSHLKVAKQAAVESAILLKNDKEVLPLQESLKTIAVVGPMANAPYEQLGTWIFDGEKAHTQTPLNAIKEIVGDKVQVIYEPGLAYSREKNPAGVAKAAAVAARADVILAFVGEEAILSGEAHCLADLNLQGDQSALITALAKTGKPVVTIVMAGRPLTIGQEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKTPVTFPKMVGQIPVYYAHNNTGRPATRNEVLLDDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNVKLSSASLKKDDVLTVTFDLENTGKYKGTEVAQLYIQDKVGSVTRPVKELKRFTRVTLKPGEKKNVSFELPVSELAFWNIDMVKVVEPGDFGLWVATDSQSGEEVFFKVVD
ACACAAGTGGCAAATAAAGGTAGCGATGCGGCAACCGAGAAAAAAGTAGAGTCTCTTTTATCCAGAATGACCCTTGAAGAGAAAATCGGTCAGATGAACCAGATTACCTCTTACGGGAATATTGAGGATATGAGTAGTTTAATTAAGAAAGGTGAAGTCGGGTCTATCCTGAATGAGGTGGATCCGGTACGTATTAATGCGTTGCAACGCGTAGCGATGGAGGAGTCCCGGTTGGGAATCCCTTTGTTGATAGCTCGCGATGTTATTCACGGGTTTAAAACCATTTTTCCCATCCCATTGGGACAAGCGGCTTCGTTCAATCCGCAGATTGCGAAAGACGGTGCACGGGTAGCGGCTATTGAGGCTTCTTCCGTAGGTATCCGTTGGACTTTTGCACCGATGATCGACATTGCCCGTGATCCTCGCTGGGGGCGCATTGCCGAAGGATGTGGTGAAGACACTTACCTGACTTCTGTAATGGGAGCTGCCATGGTAGAAGGTTTTCAGGGAGATTCTTTGAATAGTCCCACTTCCATAGCTGCCTGTCCTAAACATTTTGTGGGCTATGGTGCAGCTGAAGGCGGACGTGACTATAATTCGACATTTATTCCTGAACGTCGCCTGCGTAATGTTTACTTGCCACCGTTTGAAGCGGCAACGAAAGCGGGTGCAGCTACGTTTATGACTTCCTTTAATGATAATGATGGGATACCCTCTACCGGAAATGCTTTCATATTGAAAGATGTGCTTCGTGGCGAGTGGGGATTTGATGGTTTGGTAGTGACAGACTGGGCTTCTGCCAGCGAAATGATAAGTCATGGTTTTGCTGCCGATTCTAAAGAGGTAGCCATGAAATCAGTGAATGCTGGGGTGGATATGGAAATGGTAAGTTATACCTTTGTAAAAGAATTGCCTGCATTGATAAAAGAAGGAAAGGTGAAAGAAAGCACCATTGATGAAGCCGTTCGTAATATATTGCGCGTCAAGTATCGTCTGGGATTGTTTGATGTTCCTTATGTAGATGAAAAGCAACCCTCTGTCATGTATGATCCTTCTCATCTGAAAGTAGCTAAGCAGGCTGCTGTAGAATCGGCTATCCTGTTGAAGAATGATAAAGAAGTACTGCCGTTACAGGAGTCTCTGAAAACCATTGCTGTGGTAGGACCTATGGCCAATGCGCCTTATGAACAATTGGGTACCTGGATCTTTGATGGTGAGAAAGCTCATACTCAGACACCACTGAATGCTATTAAGGAAATAGTTGGCGACAAAGTACAGGTGATTTATGAACCCGGATTAGCTTATAGCCGTGAGAAAAATCCGGCAGGCGTAGCAAAAGCTGCTGCTGTTGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAAGCCATTCTTTCGGGTGAAGCACACTGTCTGGCAGATTTGAATCTTCAGGGTGATCAAAGTGCTTTGATTACGGCTTTGGCTAAGACAGGTAAACCTGTAGTAACCATTGTGATGGCAGGTCGTCCGTTGACTATCGGTCAGGAAGTGGAAGAATCAACAGCTGTTCTTTATTCATTCCATCCGGGTACGATGGGTGGACCGGCATTGGCCGATCTGCTGTGGGGTAAGGCGGTTCCAAGTGGAAAAACACCGGTTACTTTCCCGAAGATGGTAGGACAAATTCCGGTATATTATGCTCATAACAATACCGGGCGGCCGGCTACACGTAATGAGGTGTTGCTGGATGATATTGCTGTTGAGGCTGGACAAACTTCATTGGGATGTACTTCTTTCTATATGGATGCCGGTTTTGATCCTTTATTCCCATTTGGCTATGGCTTGTCGTATACAACGTTCAAGTATAGTAATGTCAAACTTTCATCAGCGTCATTGAAGAAAGATGATGTATTGACTGTGACATTTGATCTGGAAAATACAGGTAAATATAAGGGGACGGAAGTTGCTCAATTGTATATACAAGATAAGGTTGGTTCTGTAACTCGTCCGGTGAAAGAACTGAAACGTTTTACTCGGGTAACCTTGAAACCGGGCGAGAAAAAGAATGTTTCGTTTGAACTACCCGTTAGTGAACTTGCATTTTGGAACATCGATATGGTGAAAGTTGTGGAACCCGGAGACTTTGGACTTTGGGTGGCAACAGACAGCCAATCGGGAGAAGAAGTTTTCTTTAAGGTGGTAGAT
Clone ID: CMR200130.106 (CMR200130.32-851.P(851).E556A.pMCSG68)
SDSNVDFNKDWKFVLKDSAHYSYTSYVPGDEWKKVNLPHDWSVGLPYDSISGEGCVAFLQGGIGWYSKSFPTTISANQKCYIVFDGVYNNSEYWINGKKLGYHLSGYAPFYFDVTDYLNPNEDNRMTVRVDHSHYADSRWYTGSGIYRDVKMIVTDRLHIPVWGTFVTTPVVTDKYAKVNNQITVRNSYSEPRTAVVEIVYKDNKGNIAAFEVFSIKLNAGEEKIIDIVSEIKQPDLWSVEIPVLYTAETRIKNGDEVISENTVRFGIRTFHFDADKGFFLNGKNMKIKGVCLHHDAGIVGTAMIRDVWYRRLKTLKEGGCNAIRLSHNPGADEFLSLCDEIGLLVQEEFFDEWDYPKDKRLNMKETVEDYPTHGYCEHFQEWAERDLKNVMRRSRNHACIFQWSIGNEIEWTYTGCREATGFFGADSNGNYFWNQPPYSKEKIREMWKIQPKQAYDIGRTAQKLAAWTRQMDTTRVVTANCILPSISFETGYIDALDVAGFSYRRVMYDYAKKNYPDKPIMGTENLGQWHEWKAVIERDFVPGMFIWTGVDYLGESGSRLSRWPQKSIGCGLLDMCGYVKPSYDMMKSLWTDKPFIAIYSQTPDKSSYLQVKDGFTDKKGHEWDRRLWVWDDVNSHWNYQKGDSVIVEIYSNCDEVELFVNGKSMGKKYIDDFEDHIYKWAVQYKPGTITAKGKNKLGNTTTAIRTSGKEHSILLAVDKQSIAANGKDVLHVTAQLTDKKGNPVKTTEQMLKFNIDGEYRLLGIDNGNVKNVSPYQSKEIMTYQGRCMLMLQSTEKTSVLNISAETSELQSNKLTINIK
TCTGATTCAAATGTTGATTTCAATAAAGATTGGAAATTCGTACTGAAAGATTCTGCTCATTATTCATATACTTCTTATGTCCCTGGTGATGAATGGAAGAAAGTGAACCTGCCACACGACTGGAGTGTTGGTCTGCCTTACGACTCCATCTCTGGCGAAGGGTGTGTAGCTTTCCTTCAGGGAGGAATAGGATGGTATAGCAAATCATTTCCCACAACAATCAGCGCAAATCAGAAATGCTATATAGTGTTCGATGGAGTATATAATAATTCTGAGTATTGGATAAATGGCAAAAAACTTGGATATCATCTTTCGGGATATGCTCCTTTTTATTTTGATGTCACAGACTATCTCAATCCCAATGAGGATAACCGCATGACTGTAAGGGTCGACCACAGCCATTATGCCGACAGCAGATGGTACACCGGTTCAGGTATATACAGGGATGTGAAAATGATTGTAACCGACAGACTGCATATTCCGGTTTGGGGAACATTTGTCACTACTCCCGTGGTTACTGATAAATATGCTAAAGTAAACAACCAAATTACCGTGCGCAACAGTTACTCTGAACCCAGAACAGCTGTTGTTGAGATAGTGTATAAAGATAATAAAGGCAATATCGCAGCCTTTGAGGTCTTCAGTATAAAACTGAATGCTGGTGAGGAGAAAATTATCGACATCGTATCGGAGATAAAACAGCCGGATTTGTGGAGCGTCGAGATACCAGTCCTCTATACAGCCGAGACCCGTATTAAGAATGGCGATGAAGTCATTTCTGAAAACACTGTCAGGTTCGGTATACGAACATTCCACTTTGATGCAGACAAAGGTTTCTTCCTTAACGGAAAAAATATGAAGATAAAAGGAGTATGCCTGCATCATGATGCCGGTATAGTTGGCACAGCAATGATACGCGATGTGTGGTACCGACGTCTGAAAACCCTTAAGGAAGGAGGATGTAACGCCATCCGCCTTTCGCACAATCCGGGAGCGGATGAGTTTCTGTCTTTGTGCGATGAGATAGGTCTTCTGGTCCAGGAAGAGTTCTTCGATGAGTGGGATTATCCCAAAGATAAAAGGCTCAATATGAAGGAAACGGTAGAAGACTATCCTACTCATGGTTATTGTGAGCATTTCCAGGAATGGGCTGAAAGGGATTTGAAAAACGTAATGAGGAGAAGCCGTAATCATGCCTGTATCTTCCAGTGGAGTATAGGTAATGAAATAGAATGGACTTATACCGGATGCCGTGAGGCAACAGGTTTCTTTGGAGCCGATTCCAACGGTAATTACTTCTGGAACCAGCCTCCATACTCTAAAGAAAAAATCAGAGAAATGTGGAAAATCCAGCCTAAACAAGCATACGACATTGGTCGTACAGCGCAAAAATTAGCAGCATGGACACGCCAGATGGATACTACACGAGTGGTTACCGCCAACTGCATCCTGCCTTCCATAAGTTTTGAGACAGGATATATCGATGCACTTGATGTGGCTGGTTTCAGCTACAGACGCGTGATGTATGATTATGCTAAGAAGAATTATCCTGACAAACCTATAATGGGTACAGAAAATCTTGGTCAGTGGCACGAATGGAAGGCGGTGATTGAAAGAGATTTCGTTCCGGGTATGTTTATATGGACAGGAGTCGATTATCTGGGAGAAAGTGGAAGCCGCCTTTCAAGATGGCCTCAAAAGTCAATAGGATGTGGTCTCCTGGATATGTGCGGCTATGTGAAGCCTTCGTACGACATGATGAAATCATTGTGGACTGACAAGCCTTTTATTGCTATATATTCACAGACTCCAGACAAATCTTCGTATCTCCAGGTAAAAGATGGCTTTACTGATAAGAAAGGACATGAATGGGATAGAAGATTATGGGTTTGGGATGATGTAAACTCTCACTGGAATTATCAGAAAGGTGACTCGGTAATAGTAGAAATATATTCCAATTGTGATGAAGTGGAACTTTTCGTTAACGGCAAGTCGATGGGAAAGAAGTATATAGACGATTTTGAGGATCATATCTATAAATGGGCAGTTCAGTACAAGCCTGGCACTATTACCGCAAAAGGAAAAAATAAGTTAGGTAATACCACTACAGCTATAAGGACTTCAGGCAAAGAACATTCGATATTGCTAGCGGTTGACAAACAAAGTATCGCAGCAAATGGAAAGGATGTTCTGCATGTCACAGCCCAGCTTACAGACAAAAAAGGTAATCCTGTAAAGACAACAGAACAGATGCTTAAGTTCAACATCGATGGAGAGTACCGTCTGTTGGGTATAGACAATGGAAATGTAAAGAACGTATCTCCATATCAAAGCAAGGAGATTATGACATATCAGGGAAGATGTATGCTGATGCTTCAGTCAACAGAAAAAACATCGGTACTGAATATCAGTGCAGAAACAAGTGAATTACAGTCGAATAAACTAACAATTAATATAAAA
Clone ID: CMR200130.108 (CMR200130.32-851.P(851).E440Q.pMCSG68)
SDSNVDFNKDWKFVLKDSAHYSYTSYVPGDEWKKVNLPHDWSVGLPYDSISGEGCVAFLQGGIGWYSKSFPTTISANQKCYIVFDGVYNNSEYWINGKKLGYHLSGYAPFYFDVTDYLNPNEDNRMTVRVDHSHYADSRWYTGSGIYRDVKMIVTDRLHIPVWGTFVTTPVVTDKYAKVNNQITVRNSYSEPRTAVVEIVYKDNKGNIAAFEVFSIKLNAGEEKIIDIVSEIKQPDLWSVEIPVLYTAETRIKNGDEVISENTVRFGIRTFHFDADKGFFLNGKNMKIKGVCLHHDAGIVGTAMIRDVWYRRLKTLKEGGCNAIRLSHNPGADEFLSLCDEIGLLVQEEFFDEWDYPKDKRLNMKETVEDYPTHGYCEHFQEWAERDLKNVMRRSRNHACIFQWSIGNEIEWTYTGCREATGFFGADSNGNYFWNQPPYSKEKIREMWKIQPKQAYDIGRTAQKLAAWTRQMDTTRVVTANCILPSISFETGYIDALDVAGFSYRRVMYDYAKKNYPDKPIMGTENLGQWHEWKAVIERDFVPGMFIWTGVDYLGESGSRLSRWPQKSIGCGLLDMCGYVKPSYDMMKSLWTDKPFIAIYSQTPDKSSYLQVKDGFTDKKGHEWDRRLWVWDDVNSHWNYQKGDSVIVEIYSNCDEVELFVNGKSMGKKYIDDFEDHIYKWAVQYKPGTITAKGKNKLGNTTTAIRTSGKEHSILLAVDKQSIAANGKDVLHVTAQLTDKKGNPVKTTEQMLKFNIDGEYRLLGIDNGNVKNVSPYQSKEIMTYQGRCMLMLQSTEKTSVLNISAETSELQSNKLTINIK
TCTGATTCAAATGTTGATTTCAATAAAGATTGGAAATTCGTACTGAAAGATTCTGCTCATTATTCATATACTTCTTATGTCCCTGGTGATGAATGGAAGAAAGTGAACCTGCCACACGACTGGAGTGTTGGTCTGCCTTACGACTCCATCTCTGGCGAAGGGTGTGTAGCTTTCCTTCAGGGAGGAATAGGATGGTATAGCAAATCATTTCCCACAACAATCAGCGCAAATCAGAAATGCTATATAGTGTTCGATGGAGTATATAATAATTCTGAGTATTGGATAAATGGCAAAAAACTTGGATATCATCTTTCGGGATATGCTCCTTTTTATTTTGATGTCACAGACTATCTCAATCCCAATGAGGATAACCGCATGACTGTAAGGGTCGACCACAGCCATTATGCCGACAGCAGATGGTACACCGGTTCAGGTATATACAGGGATGTGAAAATGATTGTAACCGACAGACTGCATATTCCGGTTTGGGGAACATTTGTCACTACTCCCGTGGTTACTGATAAATATGCTAAAGTAAACAACCAAATTACCGTGCGCAACAGTTACTCTGAACCCAGAACAGCTGTTGTTGAGATAGTGTATAAAGATAATAAAGGCAATATCGCAGCCTTTGAGGTCTTCAGTATAAAACTGAATGCTGGTGAGGAGAAAATTATCGACATCGTATCGGAGATAAAACAGCCGGATTTGTGGAGCGTCGAGATACCAGTCCTCTATACAGCCGAGACCCGTATTAAGAATGGCGATGAAGTCATTTCTGAAAACACTGTCAGGTTCGGTATACGAACATTCCACTTTGATGCAGACAAAGGTTTCTTCCTTAACGGAAAAAATATGAAGATAAAAGGAGTATGCCTGCATCATGATGCCGGTATAGTTGGCACAGCAATGATACGCGATGTGTGGTACCGACGTCTGAAAACCCTTAAGGAAGGAGGATGTAACGCCATCCGCCTTTCGCACAATCCGGGAGCGGATGAGTTTCTGTCTTTGTGCGATGAGATAGGTCTTCTGGTCCAGGAAGAGTTCTTCGATGAGTGGGATTATCCCAAAGATAAAAGGCTCAATATGAAGGAAACGGTAGAAGACTATCCTACTCATGGTTATTGTGAGCATTTCCAGGAATGGGCTGAAAGGGATTTGAAAAACGTAATGAGGAGAAGCCGTAATCATGCCTGTATCTTCCAGTGGAGTATAGGTAATGAAATAGAATGGACTTATACCGGATGCCGTGAGGCAACAGGTTTCTTTGGAGCCGATTCCAACGGTAATTACTTCTGGAACCAGCCTCCATACTCTAAAGAAAAAATCAGAGAAATGTGGAAAATCCAGCCTAAACAAGCATACGACATTGGTCGTACAGCGCAAAAATTAGCAGCATGGACACGCCAGATGGATACTACACGAGTGGTTACCGCCAACTGCATCCTGCCTTCCATAAGTTTTGAGACAGGATATATCGATGCACTTGATGTGGCTGGTTTCAGCTACAGACGCGTGATGTATGATTATGCTAAGAAGAATTATCCTGACAAACCTATAATGGGTACAGAAAATCTTGGTCAGTGGCACGAATGGAAGGCGGTGATTGAAAGAGATTTCGTTCCGGGTATGTTTATATGGACAGGAGTCGATTATCTGGGAGAAAGTGGAAGCCGCCTTTCAAGATGGCCTCAAAAGTCAATAGGATGTGGTCTCCTGGATATGTGCGGCTATGTGAAGCCTTCGTACGACATGATGAAATCATTGTGGACTGACAAGCCTTTTATTGCTATATATTCACAGACTCCAGACAAATCTTCGTATCTCCAGGTAAAAGATGGCTTTACTGATAAGAAAGGACATGAATGGGATAGAAGATTATGGGTTTGGGATGATGTAAACTCTCACTGGAATTATCAGAAAGGTGACTCGGTAATAGTAGAAATATATTCCAATTGTGATGAAGTGGAACTTTTCGTTAACGGCAAGTCGATGGGAAAGAAGTATATAGACGATTTTGAGGATCATATCTATAAATGGGCAGTTCAGTACAAGCCTGGCACTATTACCGCAAAAGGAAAAAATAAGTTAGGTAATACCACTACAGCTATAAGGACTTCAGGCAAAGAACATTCGATATTGCTAGCGGTTGACAAACAAAGTATCGCAGCAAATGGAAAGGATGTTCTGCATGTCACAGCCCAGCTTACAGACAAAAAAGGTAATCCTGTAAAGACAACAGAACAGATGCTTAAGTTCAACATCGATGGAGAGTACCGTCTGTTGGGTATAGACAATGGAAATGTAAAGAACGTATCTCCATATCAAAGCAAGGAGATTATGACATATCAGGGAAGATGTATGCTGATGCTTCAGTCAACAGAAAAAACATCGGTACTGAATATCAGTGCAGAAACAAGTGAATTACAGTCGAATAAACTAACAATTAATATAAAA
Clone ID: CMR200135.107 (CMR200135.22-812.P(812).Y483A.pMCSG68)
QRHEQLLETGWKFHKGETNGAETVSFNDSQWESVCIPHDWAIYGPFDRNNDLQNVAITQNLEKQASVKTGRTGGLPYVGVGWYRTRFDADPDKKTTLVFDGAMSEARVYVNGKEACFWPFGYNSFHCDITELLHKEGKDNVLAVRLENRPQSSRWYPGAGLYRNVHLITAEKIHVPVWGTQVTTPHVANDYASVCLRTSLQNVGKEEITIETEILDPNGKKVSFKKNSGRINHGQPFTQNFIVENPQLWSPETPFLYQAVSKIYANGKLTDTYTTRFGIRSIEFVADKGFFLNGQHRKFQGVCNHHDLGPLGAAINVSALRHQLTLLKDMGCDAIRTAHNMPAPELVRLCDEMGFMMMIEPFDEWDIAKCENGYHRYFNEWAEKDMVNMLRQYRNNPCVVMWSIGNEVPTQCSSEGYKVAKFLQDICHREDPTRPVTCGMDQVSCVLDNGFAAMLDIPGFNYRAHRYEEAYQRLPQNLVLGSETSSTVSSRGVYKFPAERKADAKYEDHQSSSYDLEYCSWSNIPDIDFALADDHQWTLGQFVWTGFDYLGEPSPYDTDAWPNHSSMFGIIDLASLPKDRYYLYRSIWNKQAETLHILPHWNWEGREGKEVPVFVYTNYPTAELFINGKSYGKQTKNNQSVENRYRLMWHNAIYEPGEVKVVAYDEHGTAKAEKIIRTAGKPHHIELVSSRQSLTADGKDLAYVTVRVVDKDGNLCPTDMRLVKFKVKGAGSYKASANGDPTCLDLFHLPQMHAFNGMLTAIVQSGKEAGTLELQVTAKGLKSGKIQIEVK
CAGCGACATGAACAACTCTTGGAAACCGGCTGGAAATTCCACAAAGGAGAAACCAATGGAGCTGAAACTGTTTCATTTAATGATTCTCAATGGGAATCTGTCTGTATTCCACACGACTGGGCCATTTATGGACCGTTTGACCGTAATAATGATTTACAAAATGTAGCCATTACTCAGAACTTGGAGAAACAGGCATCTGTCAAGACCGGACGTACCGGAGGACTTCCTTATGTGGGAGTAGGATGGTATCGCACCCGTTTCGATGCAGACCCTGACAAAAAGACAACACTGGTTTTTGATGGAGCCATGAGTGAAGCCCGCGTGTATGTCAATGGAAAAGAAGCCTGCTTCTGGCCTTTCGGTTACAATTCCTTCCATTGTGACATTACTGAGCTTCTGCACAAAGAAGGAAAAGACAATGTATTGGCTGTACGTCTGGAAAACCGTCCTCAATCTTCCCGCTGGTATCCGGGAGCCGGACTTTACCGGAATGTCCATCTGATTACTGCAGAAAAAATACATGTACCTGTATGGGGAACACAGGTTACCACCCCACACGTAGCTAATGACTATGCTTCTGTTTGCCTTCGTACCTCTTTACAGAATGTGGGAAAAGAAGAAATTACCATAGAAACAGAAATACTGGACCCGAACGGGAAAAAAGTTTCTTTCAAGAAGAACAGCGGACGCATCAATCACGGGCAACCGTTTACACAAAATTTCATTGTGGAAAACCCGCAATTGTGGTCACCTGAAACACCGTTCTTATATCAGGCCGTATCTAAAATCTATGCCAACGGAAAACTTACAGATACTTATACCACCCGCTTTGGTATCCGTTCCATCGAATTTGTAGCCGACAAGGGCTTTTTCCTGAACGGCCAGCACCGTAAATTCCAGGGGGTATGCAACCACCACGACTTAGGTCCTTTAGGAGCTGCCATCAACGTATCGGCTCTACGCCACCAGCTTACATTATTAAAAGACATGGGCTGCGATGCCATTCGTACCGCACACAACATGCCGGCACCCGAGCTTGTCAGACTCTGCGATGAAATGGGATTCATGATGATGATTGAGCCTTTCGATGAATGGGACATTGCCAAGTGTGAAAACGGATACCACCGCTATTTCAACGAATGGGCCGAAAAAGACATGGTAAACATGCTACGGCAATACCGGAATAATCCCTGTGTGGTGATGTGGAGTATCGGTAATGAAGTACCCACCCAATGCAGCAGTGAAGGATACAAAGTAGCCAAGTTCCTGCAAGACATTTGCCATCGGGAAGACCCTACCCGTCCGGTTACCTGCGGCATGGACCAGGTTAGTTGTGTACTCGACAACGGATTTGCGGCCATGCTCGACATTCCGGGATTCAATTATCGCGCACACCGCTATGAAGAAGCTTACCAACGCCTGCCTCAAAATCTTGTATTAGGCTCAGAAACCTCTTCTACCGTCAGTTCACGCGGTGTATACAAATTCCCGGCAGAGCGTAAAGCCGATGCAAAATACGAAGACCATCAGTCTTCTTCTTACGACTTGGAATACTGCTCCTGGTCTAACATTCCCGATATAGACTTTGCTCTGGCTGATGACCACCAATGGACTTTGGGGCAGTTTGTCTGGACAGGTTTTGATTATCTGGGTGAACCCAGTCCATACGATACGGATGCATGGCCCAACCACAGCTCTATGTTCGGTATTATCGACCTGGCTTCCTTACCCAAAGACCGGTACTATTTATACCGCAGCATATGGAACAAGCAAGCTGAAACACTTCATATTCTTCCTCATTGGAACTGGGAGGGCAGAGAAGGAAAAGAAGTACCTGTATTCGTCTATACCAACTATCCGACAGCCGAACTTTTCATCAACGGAAAAAGTTATGGGAAACAGACGAAGAACAACCAAAGCGTAGAGAACCGTTACCGCCTGATGTGGCACAACGCCATTTACGAACCGGGAGAAGTAAAAGTCGTGGCATACGATGAACACGGTACGGCTAAAGCAGAAAAGATAATCCGCACGGCAGGCAAACCTCACCATATTGAATTGGTTTCTTCACGCCAGTCGCTCACAGCCGATGGAAAAGATTTGGCTTACGTAACCGTACGTGTTGTGGACAAAGACGGAAATCTCTGCCCCACAGATATGCGCTTGGTGAAATTTAAAGTAAAAGGAGCTGGAAGCTACAAAGCCTCAGCCAATGGAGATCCAACTTGTCTGGATTTGTTCCACCTGCCTCAGATGCACGCCTTCAACGGCATGCTGACTGCAATTGTGCAATCAGGAAAAGAAGCAGGTACCCTTGAGTTACAAGTCACCGCAAAAGGGCTGAAATCAGGAAAGATACAAATCGAAGTAAAA
Clone ID: CMR200148.108 (CMR200148.28-761.P(761).D289A.pMCSG68)
KDAEMDRFISDLMGRMTLQEKLGQLNLPAGNDLVSGAVKNSKMAEAIRAGEVGGFFNVKGVDKIYQMQRMAVEETRLGIPLIVGADVIHGYETIFPIPLALSCSWDTAAVTRMARISATEASADGISWTFSPMVDICRDARWGRIAEGSGEDPYLGALMAGAYVRGYQGDGMKQNNEIMACVKHFALYGASESGRDYNSVDMSRNLMYNVYLAPYKGAVEAGVGSVMSSFNTINGVPATADKWLLTDLLRNEWGFTGFVVTDYNSIGEMKTHGVADLKEASARALNAGTDMDMVAHGFLHTLEASLKEKAVTQERIDEACRRVLEAKYKLGLFENPYKYCDTLRGRKELFTEANRKAAREIAAETFVLLKNEGKLLPLQKKGRIALIGPMADAQNNMCGTWNMDCQTDRHVTMYEAFRRAVGDKATVSYAKGSNVYYSEHIEKGAVEPRPLTRGDDRQLRAEALRVAASADVIVAALGESAEMSGESSSRTDIQIPDAQKDLLKALIATGKPVVLALFTGRPLDLCWESEHVPAILNVWFAGSEAGDAIADVMFGEVSPSGKLTTSFPRAVGQLPLYYNHLNTGRPDTDDTTFNRYGSNYIDQSNEPLYPFGYGLSYTTFRYGNLQLSAERMAKGGQLKVTVPVTNSGECDGVEIVQLYLHDVYAEISRPVKELKAFRRVALKKGETQNVEFVLDEDDLKYYNSRLEYGYEPGEFEVMVGPDSRNVQHATFVAE
AAGGATGCGGAGATGGACCGCTTTATCAGTGACCTGATGGGAAGGATGACCTTGCAGGAAAAGTTAGGACAGTTGAATCTGCCGGCTGGGAATGACCTGGTGTCGGGAGCAGTGAAGAACAGCAAGATGGCAGAAGCTATCCGAGCTGGTGAGGTCGGCGGCTTTTTCAATGTGAAGGGAGTGGATAAGATTTACCAGATGCAGCGTATGGCGGTGGAGGAAACTCGTCTGGGAATTCCTTTGATAGTGGGTGCCGATGTGATTCACGGGTACGAAACAATCTTCCCGATTCCGTTGGCCCTGTCTTGTAGCTGGGATACGGCGGCGGTGACACGTATGGCACGTATTTCTGCCACGGAAGCCAGTGCCGATGGAATCAGCTGGACCTTCAGTCCGATGGTAGACATCTGTCGGGATGCCCGCTGGGGACGTATTGCAGAAGGAAGTGGAGAGGACCCGTACCTCGGGGCGTTGATGGCTGGAGCCTATGTGCGCGGTTATCAGGGTGACGGCATGAAGCAGAACAATGAAATCATGGCCTGTGTGAAGCACTTTGCGCTGTATGGAGCTTCGGAATCGGGACGTGACTACAATTCGGTGGATATGAGTCGAAACCTGATGTATAATGTGTACCTGGCTCCTTATAAAGGGGCGGTGGAAGCCGGAGTGGGTTCGGTGATGAGCTCGTTCAATACCATCAACGGGGTACCTGCTACAGCTGACAAATGGCTGCTGACGGATTTGCTCCGCAATGAGTGGGGGTTCACGGGGTTTGTGGTGACCGACTACAATTCGATTGGTGAGATGAAGACTCATGGGGTGGCCGACTTGAAGGAGGCTTCTGCACGGGCGTTGAATGCAGGAACGGACATGGATATGGTGGCACATGGTTTCTTGCATACGCTGGAAGCTTCATTGAAGGAGAAGGCCGTGACGCAGGAGCGGATTGACGAGGCTTGTCGTCGGGTATTGGAAGCCAAGTATAAGTTAGGATTGTTTGAAAATCCTTATAAGTATTGTGATACGCTTCGGGGACGCAAGGAATTGTTTACGGAGGCGAATCGTAAAGCGGCACGTGAGATTGCGGCTGAAACGTTTGTGCTGTTGAAGAACGAGGGTAAGTTGTTGCCTTTGCAGAAAAAAGGACGCATTGCATTGATTGGGCCGATGGCTGATGCGCAGAACAATATGTGCGGCACGTGGAACATGGATTGTCAGACAGACCGTCATGTGACGATGTACGAAGCTTTCCGTCGTGCGGTAGGTGATAAGGCTACGGTTTCTTATGCCAAGGGAAGTAATGTGTATTATAGTGAGCATATTGAGAAAGGGGCGGTCGAACCTCGTCCGCTGACACGTGGCGATGACCGTCAGTTGCGGGCTGAGGCTTTGCGCGTGGCGGCTTCTGCCGATGTGATTGTGGCCGCATTAGGTGAGAGTGCTGAGATGAGCGGAGAGTCTTCTTCTCGTACAGATATTCAGATTCCGGATGCGCAGAAAGATTTGTTGAAGGCATTGATAGCTACCGGAAAGCCGGTGGTACTGGCTTTGTTTACCGGTCGTCCGCTGGATTTATGCTGGGAGTCTGAGCATGTTCCGGCTATCCTGAACGTGTGGTTTGCCGGCAGTGAAGCGGGTGATGCCATTGCCGATGTGATGTTTGGAGAAGTATCTCCTTCGGGTAAGCTGACTACGAGTTTCCCACGTGCGGTGGGACAGTTGCCGCTTTATTATAATCACCTGAATACGGGTCGTCCGGATACGGATGACACTACTTTCAATCGTTATGGCAGCAATTACATCGACCAGAGTAATGAACCGCTTTATCCTTTTGGCTATGGTTTGAGTTATACCACTTTCCGTTACGGTAATTTGCAGTTGAGTGCGGAGCGTATGGCCAAGGGTGGGCAGTTGAAGGTAACCGTGCCTGTAACCAATTCCGGCGAGTGTGACGGAGTAGAGATTGTGCAGTTGTATCTTCACGATGTGTATGCAGAAATCTCCCGTCCGGTGAAGGAGCTGAAAGCTTTCCGCCGTGTGGCCCTTAAAAAGGGAGAGACACAGAATGTAGAGTTTGTACTCGATGAGGATGATTTGAAGTATTATAATTCTCGTCTGGAATATGGATATGAACCGGGAGAGTTTGAAGTGATGGTGGGTCCGGACAGCCGGAATGTGCAGCACGCGACTTTTGTGGCTGAA