Clone ID: APC113179.130 (APC113179.16-243.P(247)..pMCSG68)
EKLIQDREGISSKKFPIEDKELLFRYEQLYTGAVNDVMREFCLLEQALPGRIKPLREYHSVAGFAFTVKSAPNVKIKGEMEYRTQMLDEMQEDHFVVWDTSRDDKATLWGGVMTATAKGKKLKAACIDGGIRDTHQILNADFPVFYEYRISNGSLGRCLITHYQIPIKIGDVTIKPGDIILGDIDGVLVVPREIAYEVLLRSEEIRENEKKIFSWVQNGDSVQEITAK
GAAAAATTGATTCAGGATCGTGAAGGAATATCTTCGAAAAAGTTTCCTATAGAGGATAAAGAATTATTATTTCGATACGAGCAGTTGTATACAGGGGCCGTAAACGATGTGATGCGTGAATTTTGCCTTTTGGAGCAAGCCTTACCCGGTAGGATTAAACCTTTACGCGAATATCATTCTGTTGCCGGTTTTGCCTTTACTGTCAAAAGTGCACCAAACGTAAAGATAAAGGGTGAGATGGAATATCGAACCCAGATGCTGGATGAGATGCAGGAAGACCATTTTGTTGTCTGGGATACAAGTCGGGATGATAAAGCTACTTTGTGGGGAGGTGTAATGACGGCAACTGCTAAAGGGAAGAAACTGAAAGCCGCTTGTATAGATGGGGGAATACGGGATACACATCAGATTTTGAATGCTGATTTTCCTGTGTTTTACGAATATCGTATATCGAATGGTAGCTTGGGGCGCTGTTTGATTACTCACTATCAGATTCCAATTAAAATCGGAGATGTAACGATTAAGCCGGGTGATATAATACTTGGAGATATCGATGGAGTTTTAGTTGTACCAAGGGAAATAGCTTATGAAGTGCTTTTACGCTCGGAGGAGATACGTGAAAATGAGAAGAAGATTTTCTCTTGGGTACAAAACGGAGACAGTGTTCAGGAAATTACAGCAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,196 total letters Query= APC113179.130 Length=1089 Score E Sequences producing significant alignments: (Bits) Value 2_09D_C9_APC113179.130_T7S.seq 1790 0.0 1_06C_C9_APC113179.130_T7term.seq 1752 0.0 0_wt 1234 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L Query_1 1 TCTCGATCCCGCGAAATTAATACGACTC-ACTATAGGGGAATTGTGAGCGGATAACAATTCCCC--TCTAGAAATAATTTTGTTTAACTT 87 2 3 ...N.A.T......--........................ 40 1 1016 ......N...N.......T.........C...................................CTN....................... 927 1 953 ..........T.T.C.C.C..........--.A... 986 * E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E Query_1 88 TAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAG 177 2 41 .......................................................................................... 130 1 926 .......................................................................................... 837 N L Y F Q S N A E K L I Q D R E G I S S K K F P I E D K E Query_1 178 AACC-TGTACTTCCAATCCAATGCCGAAAAATTGATTCAGGATCGTGAAGGAATATCTTCGAAAAAGTTTCCTATAGAGGATAAAGAATT 266 2 131 ....-..................................................................................... 219 1 836 ....C..................................................................................... 747 0 1 ................................................................. 65 L L F R Y E Q L Y T G A V N D V M R E F C L L E Q A L P G R Query_1 267 ATTATTTCGATACGAGCAGTTGTATACAGGGGCCGTAAACGATGTGATGCGTGAATTTTGCCTTTTGGAGCAAGCCTTACCCGGTAGGAT 356 2 220 .......................................................................................... 309 1 746 .......................................................................................... 657 0 66 .......................................................................................... 155 I K P L R E Y H S V A G F A F T V K S A P N V K I K G E M E Query_1 357 TAAACCTTTACGCGAATATCATTCTGTTGCCGGTTTTGCCTTTACTGTCAAAAGTGCACCAAACGTAAAGATAAAGGGTGAGATGGAATA 446 2 310 .......................................................................................... 399 1 656 .......................................................................................... 567 0 156 .......................................................................................... 245 Y R T Q M L D E M Q E D H F V V W D T S R D D K A T L W G G Query_1 447 TCGAACCCAGATGCTGGATGAGATGCAGGAAGACCATTTTGTTGTCTGGGATACAAGTCGGGATGATAAAGCTACTTTGTGGGGAGGTGT 536 2 400 .......................................................................................... 489 1 566 .......................................................................................... 477 0 246 .......................................................................................... 335 V M T A T A K G K K L K A A C I D G G I R D T H Q I L N A D Query_1 537 AATGACGGCAACTGCTAAAGGGAAGAAACTGAAAGCCGCTTGTATAGATGGGGGAATACGGGATACACATCAGATTTTGAATGCTGATTT 626 2 490 .......................................................................................... 579 1 476 .......................................................................................... 387 0 336 .......................................................................................... 425 F P V F Y E Y R I S N G S L G R C L I T H Y Q I P I K I G D Query_1 627 TCCTGTGTTTTACGAATATCGTATATCGAATGGTAGCTTGGGGCGCTGTTTGATTACTCACTATCAGATTCCAATTAAAATCGGAGATGT 716 2 580 .......................................................................................... 669 1 386 .......................................................................................... 297 0 426 .......................................................................................... 515 V T I K P G D I I L G D I D G V L V V P R E I A Y E V L L R Query_1 717 AACGATTAAGCCGGGTGATATAATACTTGGAGATATCGATGGAGTTTTAGTTGTACCAAGGGAAATAGCTTATGAAGTGCTTTTACGCTC 806 2 670 ..........T............................................................................... 759 1 296 ..........T............................................................................... 207 0 516 .......................................................................................... 605 S E E I R E N E K K I F S W V Q N G D S V Q E I T A K * H W Query_1 807 GGAGGAGATACGTGAAAATGAGAAGAAGATTTTCTCTT-GGGTACAAAACGGAGACAGTGTTCAGGAAATTACAGCAAAATAACATTGGA 895 2 760 ......................................G................................................... 849 1 206 ......................................-................................................... 118 0 606 ......................................-......................................... 684 K W I T D P N S S S V D K L A A A L E H H H H H H * D P A Query_1 896 AGTGGATAACGGATCCGAATTCGAGCTCCGTCG-ACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccac-TGAGATCCGGCTGC 983 2 850 .................................A..................................--.....T.............. 937 1 117 .................................-.......................---...............-.............. 33 A N K A R K E A E L A A A T A E Q * L A * P L G A S K R V Query_1 984 TAACAAAGCCCGAAAGGAAGC-TGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTT-- 1070 2 938 ..........NA....T....N........................................T..........N..............TG 1027 1 32 ................... 14 L R G F L L K Query_1 1071 GAGGGGTTTTTTGCTGAAA 1089 2 1028 N.................. 1046 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3377804 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 3,196 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC113179.131 (APC113179.17-243.P(247)..pMCSG68)
KLIQDREGISSKKFPIEDKELLFRYEQLYTGAVNDVMREFCLLEQALPGRIKPLREYHSVAGFAFTVKSAPNVKIKGEMEYRTQMLDEMQEDHFVVWDTSRDDKATLWGGVMTATAKGKKLKAACIDGGIRDTHQILNADFPVFYEYRISNGSLGRCLITHYQIPIKIGDVTIKPGDIILGDIDGVLVVPREIAYEVLLRSEEIRENEKKIFSWVQNGDSVQEITAK
AAATTGATTCAGGATCGTGAAGGAATATCTTCGAAAAAGTTTCCTATAGAGGATAAAGAATTATTATTTCGATACGAGCAGTTGTATACAGGGGCCGTAAACGATGTGATGCGTGAATTTTGCCTTTTGGAGCAAGCCTTACCCGGTAGGATTAAACCTTTACGCGAATATCATTCTGTTGCCGGTTTTGCCTTTACTGTCAAAAGTGCACCAAACGTAAAGATAAAGGGTGAGATGGAATATCGAACCCAGATGCTGGATGAGATGCAGGAAGACCATTTTGTTGTCTGGGATACAAGTCGGGATGATAAAGCTACTTTGTGGGGAGGTGTAATGACGGCAACTGCTAAAGGGAAGAAACTGAAAGCCGCTTGTATAGATGGGGGAATACGGGATACACATCAGATTTTGAATGCTGATTTTCCTGTGTTTTACGAATATCGTATATCGAATGGTAGCTTGGGGCGCTGTTTGATTACTCACTATCAGATTCCAATTAAAATCGGAGATGTAACGATTAAGCCGGGTGATATAATACTTGGAGATATCGATGGAGTTTTAGTTGTACCAAGGGAAATAGCTTATGAAGTGCTTTTACGCTCGGAGGAGATACGTGAAAATGAGAAGAAGATTTTCTCTTGGGTACAAAACGGAGACAGTGTTCAGGAAATTACAGCAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,192 total letters Query= APC113179.131 Length=1086 Score E Sequences producing significant alignments: (Bits) Value 1_06D_D9_APC113179.131_T7term.seq 1755 0.0 2_09E_D9_APC113179.131_T7S.seq 1663 0.0 0_wt 1229 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTC-TAGAAATAATTTTGTTTAACTTTA 89 1 1011 .......N...N..............N......................................C................N.C--... 924 1 948 ...........T.T.C.C.C...........A-... 982 2 12 ......-.N...-.................. 40 * E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E Query_1 90 AGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAA 179 1 923 .......................................................................................... 834 2 41 .......................................................................................... 130 N L Y F Q S N A K L I Q D R E G I S S K K F P I E D K E L L Query_1 180 CCTGTACTTCCAATCCAATGCCAAATTGATTCAGGATCGTGAAGGAATATCTTCGAAAAAGTTTCCTATAGAGGATAAAGAATTATTATT 269 1 833 .......................................................................................... 744 2 131 .......................................................................................... 220 0 1 .................................................................... 68 F R Y E Q L Y T G A V N D V M R E F C L L E Q A L P G R I K Query_1 270 TCGATACGAGCAGTTGTATACAGGGGCCGTAAACGATGTGATGCGTGAATTTTGCCTTTTGGAGCAAGCCTTACCCGGTAGGATTAAACC 359 1 743 .......................................................................................... 654 2 221 .......................................................................................... 310 0 69 .......................................................................................... 158 P L R E Y H S V A G F A F T V K S A P N V K I K G E M E Y R Query_1 360 TTTACGCGAATATCATTCTGTTGCCGGTTTTGCCTTTACTGTCAAAAGTGCACCAAACGTAAAGATAAAGGGTGAGATGGAATATCGAAC 449 1 653 .......................................................................................... 564 2 311 .......................................................................................... 400 0 159 .......................................................................................... 248 T Q M L D E M Q E D H F V V W D T S R D D K A T L W G G V M Query_1 450 CCAGATGCTGGATGAGATGCAGGAAGACCATTTTGTTGTCTGGGATACAAGTCGGGATGATAAAGCTACTTTGTGGGGAGGTGTAATGAC 539 1 563 .......................................................................................... 474 2 401 .......................................................................................... 490 0 249 .......................................................................................... 338 T A T A K G K K L K A A C I D G G I R D T H Q I L N A D F P Query_1 540 GGCAACTGCTAAAGGGAAGAAACTGAAAGCCGCTTGTATAGATGGGGGAATACGGGATACACATCAGATTTTGAATGCTGATTTTCCTGT 629 1 473 .......................................................................................... 384 2 491 .......................................................................................... 580 0 339 .......................................................................................... 428 V F Y E Y R I S N G S L G R C L I T H Y Q I P I K I G D V T Query_1 630 GTTTTACGAATATCGTATATCGAATGGTAGCTTGGGGCGCTGTTTGATTACTCACTATCAGATTCCAATTAAAATCGGAGATGTAACGAT 719 1 383 .......................................................................................... 294 2 581 .......................................................................................... 670 0 429 .......................................................................................... 518 I K P G D I I L G D I D G V L V V P R E I A Y E V L L R S Query_1 720 T-AAGCCGGGTGATATAATACTTGGAGATATCGA-TGGAGTTTTAG-TTGTACCAA-GGGAAATAGCTTATGAAGTGCTTTTACGCTCGG 805 1 293 .-...T............................-...........-.........-................................. 208 2 671 .A...T............................T...........T.........G................................. 760 0 519 .-................................-...........-.........-................................. 604 E E I R E N E K K I F S W V Q N G D S V Q E I T A K * H W Query_1 806 AGGAGATACGTGAAAATGAGAAGAAGA-TTTTCT-CTTGGGTACAAAACGGAGACAGTGTTCAGG-AAATTACAGCAAAATAACATTGGA 892 1 207 ...........................-......-..............................-........................ 121 2 761 ...........................N......C......................N.....N.A........................ 850 0 605 ...........................-......-..............................-.............. 681 K W I T D P N S S S V D K L A A A L E H H H H H H * D P A Query_1 893 AGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCG-CACTCGAGcaccaccaccaccaccacTG-AGATCCGGCTGC 980 1 120 ................................................-............................-............ 33 2 851 ......................N............N............C..N...................N.....A......N..... 940 A N K A R K E A E L A A A T A E Q * L A * P L G A S K R V L Query_1 981 TAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG-CTGCCACCGCTGAGCAATAACTAGCATAACCCCTT-GGGGCCTCTAAACGGGTCTTG 1068 1 32 ................ 17 2 941 ..N..N.............N..T.A.......T..T.T..............NNN......N......G....N..TNN........... 1030 R G F L L Query_1 1069 AGGGG--TTTTTTGCTGA 1084 2 1031 NN...GT........... 1048 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3364080 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 3,192 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC113179.132 (APC113179.18-243.P(247)..pMCSG68)
LIQDREGISSKKFPIEDKELLFRYEQLYTGAVNDVMREFCLLEQALPGRIKPLREYHSVAGFAFTVKSAPNVKIKGEMEYRTQMLDEMQEDHFVVWDTSRDDKATLWGGVMTATAKGKKLKAACIDGGIRDTHQILNADFPVFYEYRISNGSLGRCLITHYQIPIKIGDVTIKPGDIILGDIDGVLVVPREIAYEVLLRSEEIRENEKKIFSWVQNGDSVQEITAK
TTGATTCAGGATCGTGAAGGAATATCTTCGAAAAAGTTTCCTATAGAGGATAAAGAATTATTATTTCGATACGAGCAGTTGTATACAGGGGCCGTAAACGATGTGATGCGTGAATTTTGCCTTTTGGAGCAAGCCTTACCCGGTAGGATTAAACCTTTACGCGAATATCATTCTGTTGCCGGTTTTGCCTTTACTGTCAAAAGTGCACCAAACGTAAAGATAAAGGGTGAGATGGAATATCGAACCCAGATGCTGGATGAGATGCAGGAAGACCATTTTGTTGTCTGGGATACAAGTCGGGATGATAAAGCTACTTTGTGGGGAGGTGTAATGACGGCAACTGCTAAAGGGAAGAAACTGAAAGCCGCTTGTATAGATGGGGGAATACGGGATACACATCAGATTTTGAATGCTGATTTTCCTGTGTTTTACGAATATCGTATATCGAATGGTAGCTTGGGGCGCTGTTTGATTACTCACTATCAGATTCCAATTAAAATCGGAGATGTAACGATTAAGCCGGGTGATATAATACTTGGAGATATCGATGGAGTTTTAGTTGTACCAAGGGAAATAGCTTATGAAGTGCTTTTACGCTCGGAGGAGATACGTGAAAATGAGAAGAAGATTTTCTCTTGGGTACAAAACGGAGACAGTGTTCAGGAAATTACAGCAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,354 total letters Query= APC113179.132 Length=1083 Score E Sequences producing significant alignments: (Bits) Value 2_09F_E9_APC113179.132_T7S.seq 1680 0.0 1_06E_E9_APC113179.132_T7term.seq 1523 0.0 0_wt 1223 0.0 E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 12 CGAAATTAATAC-GACTCACTATAGGGG--AATTGTGAG-CGGATAAC-AATT-CCCCT-CTAGAAATAA-TTTTGTTTAAC--TTTAAG 91 2 10 ....N.....-.N........-...........--...N.. 46 1 1011 ..........T.N...C.C..NN.....GA.....N...GN.......C....-N....N.......N..A...N....T..CTN..... 923 E G D I H M H H H H H H S S G V D L W S H P Q F E K Query_1 92 AA-GGA-GATATAC-ATATG--CACCAT-CATCATCAT-CATTCTT-CTGGTGTAGATCTGTGGTCTCATCCG--CAGTTCGAAAA-GGG 170 2 47 ..-...-.......-.....--......-.........-.......-..........................--...........-... 125 1 922 ..A...N.......C..N.TGC.C....N.........T.......TN...N...N...C............CGC...........A... 833 G T E N L Y F Q S N A L I Q D R E G I S S K K F P I E D K Query_1 171 TACC-GAGAACCTGTACTTCC-AATCC-AATGCCTTGATTCAGGATCGTGAAGGAATATCTTCGAAAAA-GTTTCCTATAGAGGATAAA- 255 2 126 ....-................-.....-.........................................-...................- 210 1 832 ....C................C.....C.........................................A...................A 743 0 1 ...................................-...................- 54 E L L F R Y E Q L Y T G A V N D V M R E F C L L E Q A L P G Query_1 256 GAATTATTATTTCGATACGAGCAGTTGTATACAGGGGCCGTAAACGATGTGATGCGTGAATTTTGCCTTTTGGAGCAAGCCTTACCCGGT 345 2 211 ..................N....................................................................... 300 1 742 ....................................C..................................................... 653 0 55 .......................................................................................... 144 R I K P L R E Y H S V A G F A F T V K S A P N V K I K G E M Query_1 346 AGGATTAAACCTTTACGCGAATATCATTCTGTTGCCGGTTTTGCCTTTACTGTCAAAAGTGCACCAAACGTAAAGATAAAGGGTGAGATG 435 2 301 .......................................................................................... 390 1 652 .......................................................................................... 563 0 145 .......................................................................................... 234 E Y R T Q M L D E M Q E D H F V V W D T S R D D K A T L W G Query_1 436 GAATATCGAACCCAGATGCTGGATGAGATGCAGGAAGACCATTTTGTTGTCTGGGATACAAGTCGGGATGATAAAGCTACTTTGTGGGGA 525 2 391 .......................................................................................... 480 1 562 .......................................................................................... 473 0 235 .......................................................................................... 324 G V M T A T A K G K K L K A A C I D G G I R D T H Q I L N A Query_1 526 GGTGTAATGACGGCAACTGCTAAAGGGAAGAAACTGAAAGCCGCTTGTATAGATGGGGGAATACGGGATACACATCAGATTTTGAATGCT 615 2 481 .......................................................................................... 570 1 472 .......................................................................................... 383 0 325 .......................................................................................... 414 D F P V F Y E Y R I S N G S L G R C L I T H Y Q I P I K I G Query_1 616 GATTTTCCTGTGTTTTACGAATATCGTATATCGAATGGTAGCTTGGGGCGCTGTTTGATTACTCACTATCAGATTCCAATTAAAATCGGA 705 2 571 .......................................................................................... 660 1 382 .......................................................................................... 293 0 415 .......................................................................................... 504 D V T I K P G D I I L G D I D G V L V V P R E I A Y E V L Query_1 706 GATGTAACGATTAAGCCGGGTGATATAATACTTGGAGATATCGATGGAG-TTTTAGTTGTACCAAGGGAAATAGCTTATGAAGTGC-TTT 793 2 661 ...............T.................................T....................................T... 750 1 292 ...............T.................................-....................................-... 205 0 505 .................................................-....................................-... 592 L R S E E I R E N E K K I F S W V Q N G D S V Q E I T A K * Query_1 794 TACGCTCGGAGGAGATACGTG-AAAATGAGAAGAAGATTTTCTCTTGGGTACAAAACGGAGACAGTGTTCAGGAAATTACAGCAAAATAA 882 2 751 .....................A........................-.....N..................................... 839 1 204 .....................-.................................................................... 116 0 593 .....................-................................................................. 678 H W K W I T D P N S S S V D K L A A A L E H H H H H H * D Query_1 883 CATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCAC-TCGAGcaccaccaccaccacca-cTGAGATC 970 2 840 ........................-.N.........................T.....T..................N...N........ 928 1 115 ................................................A.........-......................-........ 28 P A A N K A R K E A E L A A A T A E Q * L A * P L G A S K R Query_1 971 CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGG 1060 2 929 .........N.-.........N.......................N.A....N....N....N.NN..N.........G..TCT.....T 1017 1 27 ...........-......A.. 8 V L R G F L L K Query_1 1061 TCTTGAGG-GGTTTTTTGCTGAAA 1083 2 1018 NT.G....N.N............. 1041 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3527502 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 3,354 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115027.105 (APC115027.30-870.P(888).E423A.pMCSG68)
HESKTINDSWKFYQGECKAAASATYDDSAWNSIHLPHTWNTDAYTEKKYYQGTGWYRRTLTLPQNWQTKQVFLKLDAASKAAAIYMNGRKIGEHNGGYTACTFDITPFCSFDAPNSLAIHVDNARQDIPPISADFTFFGGIYRDVWLTAVPKQHFHLTNYGSEGIFISTPQVSEEKGTILIRGEIKNDAEQKASLELEHIVYNPDGSIAQTQKQSIQIKGGELYSFRTETSPILSPRLWTPEAPHLYRIESILRDKKTKAILDHSNHHTGFRWFSFDGKTGFSLNGKPYKLRGICRHQDQKPIGVALTDEMHRRDMKLMKEMGANFIRISHYPQDDALLEMCDKLGMLAWEEIPIIDIVPDTPGYAENCENNLREMIRQHYNHPSIITWGYMNEILLVTQRRYKKEEELKPVLERTLSLANRLEKVLKEEDSTRVSTMAFHGSNSYNEVGLGNITDIVGWNLYQGWYGGDLTGFERFLTEQHKKHPSHPMIVSEYGAGSDKRLHSLQPHAFDFSIEYQQKYLEHYLPVLEETPYICGGTHWNFIDFSSALRDESMPRINNKGLVYSDRSPKDVYYYYKAAWRQDIPVLHIASRDWTHRSGVQHGKAPVPLPVKVYTNLPEVELFIDGKSLGKQKTENYTVTFQVPFSRKEHFISAQAENKESDQSISMIEDALHINFTPIPANLNETNLRNLELAVNVGSNCFYTSDESQLTWLPDQPYTESSWGYIGGESKNSQTQVENTHDGPLFQTLRNEIEGYCFDVPNGVYKVEFLFTDIFRENAAAVYQLDRESGVESRENIFDIVINGESVEENFSPCRESGYFHALRKRYNITNFRNKIEIRF
CATGAGAGCAAAACGATCAACGACAGTTGGAAATTCTATCAGGGAGAATGCAAAGCTGCCGCTTCAGCAACCTATGATGACAGTGCGTGGAACAGCATACATCTCCCGCATACATGGAATACGGATGCATATACAGAAAAAAAGTATTACCAGGGAACAGGCTGGTATCGCCGTACGCTGACATTACCGCAAAATTGGCAAACCAAACAGGTATTCCTGAAACTGGATGCAGCCAGTAAGGCCGCTGCCATCTACATGAATGGACGGAAAATAGGTGAGCATAACGGTGGATATACTGCCTGCACATTCGATATCACCCCATTCTGTTCATTTGACGCTCCTAATTCTCTCGCTATCCATGTAGATAATGCCCGACAGGATATTCCCCCTATCTCTGCCGACTTTACATTCTTCGGCGGTATCTACCGGGATGTATGGCTTACAGCCGTACCAAAACAACATTTCCATTTGACGAACTATGGTTCAGAAGGAATATTTATCAGTACCCCGCAAGTATCCGAAGAAAAAGGCACAATACTTATTCGCGGAGAGATAAAGAACGATGCAGAACAGAAAGCATCTCTTGAACTGGAACATATCGTATACAATCCCGATGGAAGTATTGCCCAGACCCAGAAACAGTCTATCCAGATAAAAGGTGGAGAATTATACTCTTTCCGCACCGAAACCTCCCCTATTCTGTCCCCCCGATTATGGACACCCGAGGCCCCTCATCTCTACAGAATAGAAAGCATTCTGCGCGATAAAAAAACAAAAGCCATCCTAGACCACAGTAATCACCATACAGGCTTCCGCTGGTTTAGCTTCGATGGTAAGACCGGCTTCTCCCTCAACGGAAAACCTTATAAATTACGCGGTATTTGCCGCCATCAGGACCAAAAACCCATCGGAGTTGCCCTGACAGACGAAATGCACCGTCGTGACATGAAGTTAATGAAAGAGATGGGTGCCAACTTCATCCGCATCTCCCATTATCCGCAAGATGACGCTCTGTTGGAAATGTGCGACAAACTCGGTATGCTAGCTTGGGAAGAAATACCCATTATTGATATTGTGCCCGACACTCCCGGCTATGCAGAAAACTGCGAAAACAATCTGCGAGAAATGATACGCCAGCACTATAACCATCCCTCCATCATCACCTGGGGATACATGAATGAGATATTACTCGTCACCCAGCGCCGCTATAAAAAAGAAGAAGAACTGAAACCCGTGCTGGAACGGACATTGTCACTGGCCAATCGTTTGGAGAAAGTTCTGAAAGAAGAAGATTCCACTCGTGTCAGCACTATGGCTTTTCATGGCAGTAACAGCTACAATGAGGTAGGACTGGGCAACATCACCGATATTGTCGGTTGGAATCTTTACCAAGGCTGGTATGGAGGAGACCTCACAGGCTTCGAACGTTTCCTGACAGAGCAACACAAAAAACACCCTTCCCATCCGATGATTGTCAGCGAATACGGTGCCGGATCTGACAAGCGTCTACATTCGCTGCAACCGCACGCTTTTGATTTCAGTATCGAGTACCAGCAGAAATACCTGGAACACTACTTACCGGTATTGGAAGAAACGCCGTATATTTGTGGTGGAACTCACTGGAACTTTATCGATTTCTCTTCTGCATTGCGTGATGAATCTATGCCGCGCATCAACAATAAAGGACTGGTATACTCAGACCGCAGTCCAAAAGACGTGTACTATTACTATAAAGCCGCCTGGCGTCAAGACATTCCCGTGTTACACATTGCCAGCCGTGACTGGACGCACCGTAGCGGTGTACAACATGGAAAGGCTCCGGTTCCGCTACCCGTCAAGGTATATACCAATCTTCCGGAAGTGGAACTCTTCATTGACGGAAAATCCTTAGGTAAACAGAAAACAGAGAATTATACAGTTACATTTCAGGTACCATTCAGCCGGAAAGAGCACTTTATCTCCGCCCAAGCTGAAAACAAAGAGTCAGATCAAAGCATCTCCATGATAGAAGATGCCCTCCACATCAACTTCACCCCTATCCCTGCAAATCTGAATGAAACAAACTTACGGAATCTGGAACTTGCCGTAAATGTAGGCAGCAACTGTTTCTATACTTCCGATGAAAGCCAGCTCACGTGGCTACCCGATCAGCCCTACACTGAAAGCAGTTGGGGATATATCGGCGGAGAAAGCAAAAATAGCCAGACACAGGTTGAGAATACCCATGACGGGCCATTGTTTCAAACTCTTCGCAACGAAATTGAAGGGTATTGTTTCGATGTTCCCAACGGAGTCTACAAGGTAGAATTTCTTTTTACAGATATCTTCCGCGAAAATGCAGCAGCTGTTTACCAGTTGGACCGGGAATCCGGTGTAGAGAGCCGTGAAAATATCTTCGATATAGTTATCAATGGTGAGAGCGTTGAAGAGAACTTTTCTCCTTGCCGCGAAAGCGGTTATTTCCATGCTCTACGGAAAAGATACAATATTACAAACTTTCGGAATAAAATTGAAATACGCTTC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,533 total letters Query= APC115027.105 Length=2928 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4542 0.0 H E S K T I N D S W K F Y Q G E C K A A A S A T Y D D S A W Query_1 202 CATGAGAGCAAAACGATCAACGACAGTTGGAAATTCTATCAGGGAGAATGCAAAGCTGCCGCTTCAGCAACCTATGATGACAGTGCGTGG 291 0 1 .......................................................................................... 90 N S I H L P H T W N T D A Y T E K K Y Y Q G T G W Y R R T L Query_1 292 AACAGCATACATCTCCCGCATACATGGAATACGGATGCATATACAGaaaaaaaGTATTACCAGGGAACAGGCTGGTATCGCCGTACGCTG 381 0 91 .......................................................................................... 180 T L P Q N W Q T K Q V F L K L D A A S K A A A I Y M N G R K Query_1 382 ACATTACCGCAAAATTGGCAAACCAAACAGGTATTCCTGAAACTGGATGCAGCCAGTAAGGCCGCTGCCATCTACATGAATGGACGGAAA 471 0 181 .......................................................................................... 270 I G E H N G G Y T A C T F D I T P F C S F D A P N S L A I H Query_1 472 ATAGGTGAGCATAACGGTGGATATACTGCCTGCACATTCGATATCACCCCATTCTGTTCATTTGACGCTCCTAATTCTCTCGCTATCCAT 561 0 271 .......................................................................................... 360 V D N A R Q D I P P I S A D F T F F G G I Y R D V W L T A V Query_1 562 GTAGATAATGCCCGACAGGATATTCCCCCTATCTCTGCCGACTTTACATTCTTCGGCGGTATCTACCGGGATGTATGGCTTACAGCCGTA 651 0 361 .......................................................................................... 450 P K Q H F H L T N Y G S E G I F I S T P Q V S E E K G T I L Query_1 652 CCAAAACAACATTTCCATTTGACGAACTATGGTTCAGAAGGAATATTTATCAGTACCCCGCAAGTATCCGAAGAAAAAGGCACAATACTT 741 0 451 .......................................................................................... 540 I R G E I K N D A E Q K A S L E L E H I V Y N P D G S I A Q Query_1 742 ATTCGCGGAGAGATAAAGAACGATGCAGAACAGAAAGCATCTCTTGAACTGGAACATATCGTATACAATCCCGATGGAAGTATTGCCCAG 831 0 541 .......................................................................................... 630 T Q K Q S I Q I K G G E L Y S F R T E T S P I L S P R L W T Query_1 832 ACCCAGAAACAGTCTATCCAGATAAAAGGTGGAGAATTATACTCTTTCCGCACCGAAACCTCCCCTATTCTGTCCCCCCGATTATGGACA 921 0 631 .......................................................................................... 720 0 1906 ........... 1916 P E A P H L Y R I E S I L R D K K T K A I L D H S N H H T G Query_1 922 CCCGAGGCCCCTCATCTCTACAGAATAGAAAGCATTCTGCGCGATaaaaaaaCAAAAGCCATCCTAGACCACAGTAATCACCATACAGGC 1011 0 721 .......................................................................................... 810 F R W F S F D G K T G F S L N G K P Y K L R G I C R H Q D Q Query_1 1012 TTCCGCTGGTTTAGCTTCGATGGTAAGACCGGCTTCTCCCTCAACGGAAAACCTTATAAATTACGCGGTATTTGCCGCCATCAGGACCAA 1101 0 811 .......................................................................................... 900 K P I G V A L T D E M H R R D M K L M K E M G A N F I R I S Query_1 1102 AAACCCATCGGAGTTGCCCTGACAGACGAAATGCACCGTCGTGACATGAAGTTAATGAAAGAGATGGGTGCCAACTTCATCCGCATCTCC 1191 0 901 .......................................................................................... 990 H Y P Q D D A L L E M C D K L G M L A W E E I P I I D I V P Query_1 1192 CATTATCCGCAAGATGACGCTCTGTTGGAAATGTGCGACAAACTCGGTATGCTAGCTTGGGAAGAAATACCCATTATTGATATTGTGCCC 1281 0 991 .......................................................................................... 1080 D T P G Y A E N C E N N L R E M I R Q H Y N H P S I I T W G Query_1 1282 GACACTCCCGGCTATGCAGAAAACTGCGAAAACAATCTGCGAGAAATGATACGCCAGCACTATAACCATCCCTCCATCATCACCTGGGGA 1371 0 1081 .......................................................................................... 1170 Y M N A I L L V T Q R R Y K K E E E L K P V L E R T L S L A Query_1 1372 TACATGAATGCTATATTACTCGTCACCCAGCGCCGCTATAAAAAAGAAGAAGAACTGAAACCCGTGCTGGAACGGACATTGTCACTGGCC 1461 0 1171 ..........AG.............................................................................. 1260 0 1282 ........... 1292 N R L E K V L K E E D S T R V S T M A F H G S N S Y N E V G Query_1 1462 AATCGTTTGGAGAAAGTTCTGAAAGAAGAAGATTCCACTCGTGTCAGCACTATGGCTTTTCATGGCAGTAACAGCTACAATGAGGTAGGA 1551 0 1261 .......................................................................................... 1350 0 1213 ........... 1223 L G N I T D I V G W N L Y Q G W Y G G D L T G F E R F L T E Query_1 1552 CTGGGCAACATCACCGATATTGTCGGTTGGAATCTTTACCAAGGCTGGTATGGAGGAGACCTCACAGGCTTCGAACGTTTCCTGACAGAG 1641 0 1351 .......................................................................................... 1440 Q H K K H P S H P M I V S E Y G A G S D K R L H S L Q P H A Query_1 1642 CAACACAAAAAACACCCTTCCCATCCGATGATTGTCAGCGAATACGGTGCCGGATCTGACAAGCGTCTACATTCGCTGCAACCGCACGCT 1731 0 1441 .......................................................................................... 1530 F D F S I E Y Q Q K Y L E H Y L P V L E E T P Y I C G G T H Query_1 1732 TTTGATTTCAGTATCGAGTACCAGCAGAAATACCTGGAACACTACTTACCGGTATTGGAAGAAACGCCGTATATTTGTGGTGGAACTCAC 1821 0 1531 .......................................................................................... 1620 W N F I D F S S A L R D E S M P R I N N K G L V Y S D R S P Query_1 1822 TGGAACTTTATCGATTTCTCTTCTGCATTGCGTGATGAATCTATGCCGCGCATCAACAATAAAGGACTGGTATACTCAGACCGCAGTCCA 1911 0 1621 .......................................................................................... 1710 K D V Y Y Y Y K A A W R Q D I P V L H I A S R D W T H R S G Query_1 1912 AAAGACGTGTACTATTACTATAAAGCCGCCTGGCGTCAAGACATTCCCGTGTTACACATTGCCAGCCGTGACTGGACGCACCGTAGCGGT 2001 0 1711 .......................................................................................... 1800 V Q H G K A P V P L P V K V Y T N L P E V E L F I D G K S L Query_1 2002 GTACAACATGGAAAGGCTCCGGTTCCGCTACCCGTCAAGGTATATACCAATCTTCCGGAAGTGGAACTCTTCATTGACGGAAAATCCTTA 2091 0 1801 .......................................................................................... 1890 G K Q K T E N Y T V T F Q V P F S R K E H F I S A Q A E N K Query_1 2092 GGTAAACAGAAAACAGAGAATTATACAGTTACATTTCAGGTACCATTCAGCCGGAAAGAGCACTTTATCTCCGCCCAAGCTGAAAACAAA 2181 0 1891 .......................................................................................... 1980 0 662 ........... 672 E S D Q S I S M I E D A L H I N F T P I P A N L N E T N L R Query_1 2182 GAGTCAGATCAAAGCATCTCCATGATAGAAGATGCCCTCCACATCAACTTCACCCCTATCCCTGCAAATCTGAATGAAACAAACTTACGG 2271 0 1981 .......................................................................................... 2070 N L E L A V N V G S N C F Y T S D E S Q L T W L P D Q P Y T Query_1 2272 AATCTGGAACTTGCCGTAAATGTAGGCAGCAACTGTTTCTATACTTCCGATGAAAGCCAGCTCACGTGGCTACCCGATCAGCCCTACACT 2361 0 2071 .......................................................................................... 2160 E S S W G Y I G G E S K N S Q T Q V E N T H D G P L F Q T L Query_1 2362 GAAAGCAGTTGGGGATATATCGGCGGAGAAAGCAAAAATAGCCAGACACAGGTTGAGAATACCCATGACGGGCCATTGTTTCAAACTCTT 2451 0 2161 .......................................................................................... 2250 R N E I E G Y C F D V P N G V Y K V E F L F T D I F R E N A Query_1 2452 CGCAACGAAATTGAAGGGTATTGTTTCGATGTTCCCAACGGAGTCTACAAGGTAGAATTTCTTTTTACAGATATCTTCCGCGAAAATGCA 2541 0 2251 .......................................................................................... 2340 A A V Y Q L D R E S G V E S R E N I F D I V I N G E S V E E Query_1 2542 GCAGCTGTTTACCAGTTGGACCGGGAATCCGGTGTAGAGAGCCGTGAAAATATCTTCGATATAGTTATCAATGGTGAGAGCGTTGAAGAG 2631 0 2341 .......................................................................................... 2430 N F S P C R E S G Y F H A L R K R Y N I T N F R N K I E I R Query_1 2632 AACTTTTCTCCTTGCCGCGAAAGCGGTTATTTCCATGCTCTACGGAAAAGATACAATATTACAAACTTTCGGAATAAAATTGAAATACGC 2721 0 2431 .......................................................................................... 2520 F Query_1 2722 TTC 2724 0 2521 ... 2523 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 7225102 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 2,533 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115027.107 (APC115027.30-870.P(888).E423Q.pMCSG68)
HESKTINDSWKFYQGECKAAASATYDDSAWNSIHLPHTWNTDAYTEKKYYQGTGWYRRTLTLPQNWQTKQVFLKLDAASKAAAIYMNGRKIGEHNGGYTACTFDITPFCSFDAPNSLAIHVDNARQDIPPISADFTFFGGIYRDVWLTAVPKQHFHLTNYGSEGIFISTPQVSEEKGTILIRGEIKNDAEQKASLELEHIVYNPDGSIAQTQKQSIQIKGGELYSFRTETSPILSPRLWTPEAPHLYRIESILRDKKTKAILDHSNHHTGFRWFSFDGKTGFSLNGKPYKLRGICRHQDQKPIGVALTDEMHRRDMKLMKEMGANFIRISHYPQDDALLEMCDKLGMLAWEEIPIIDIVPDTPGYAENCENNLREMIRQHYNHPSIITWGYMNEILLVTQRRYKKEEELKPVLERTLSLANRLEKVLKEEDSTRVSTMAFHGSNSYNEVGLGNITDIVGWNLYQGWYGGDLTGFERFLTEQHKKHPSHPMIVSEYGAGSDKRLHSLQPHAFDFSIEYQQKYLEHYLPVLEETPYICGGTHWNFIDFSSALRDESMPRINNKGLVYSDRSPKDVYYYYKAAWRQDIPVLHIASRDWTHRSGVQHGKAPVPLPVKVYTNLPEVELFIDGKSLGKQKTENYTVTFQVPFSRKEHFISAQAENKESDQSISMIEDALHINFTPIPANLNETNLRNLELAVNVGSNCFYTSDESQLTWLPDQPYTESSWGYIGGESKNSQTQVENTHDGPLFQTLRNEIEGYCFDVPNGVYKVEFLFTDIFRENAAAVYQLDRESGVESRENIFDIVINGESVEENFSPCRESGYFHALRKRYNITNFRNKIEIRF
CATGAGAGCAAAACGATCAACGACAGTTGGAAATTCTATCAGGGAGAATGCAAAGCTGCCGCTTCAGCAACCTATGATGACAGTGCGTGGAACAGCATACATCTCCCGCATACATGGAATACGGATGCATATACAGAAAAAAAGTATTACCAGGGAACAGGCTGGTATCGCCGTACGCTGACATTACCGCAAAATTGGCAAACCAAACAGGTATTCCTGAAACTGGATGCAGCCAGTAAGGCCGCTGCCATCTACATGAATGGACGGAAAATAGGTGAGCATAACGGTGGATATACTGCCTGCACATTCGATATCACCCCATTCTGTTCATTTGACGCTCCTAATTCTCTCGCTATCCATGTAGATAATGCCCGACAGGATATTCCCCCTATCTCTGCCGACTTTACATTCTTCGGCGGTATCTACCGGGATGTATGGCTTACAGCCGTACCAAAACAACATTTCCATTTGACGAACTATGGTTCAGAAGGAATATTTATCAGTACCCCGCAAGTATCCGAAGAAAAAGGCACAATACTTATTCGCGGAGAGATAAAGAACGATGCAGAACAGAAAGCATCTCTTGAACTGGAACATATCGTATACAATCCCGATGGAAGTATTGCCCAGACCCAGAAACAGTCTATCCAGATAAAAGGTGGAGAATTATACTCTTTCCGCACCGAAACCTCCCCTATTCTGTCCCCCCGATTATGGACACCCGAGGCCCCTCATCTCTACAGAATAGAAAGCATTCTGCGCGATAAAAAAACAAAAGCCATCCTAGACCACAGTAATCACCATACAGGCTTCCGCTGGTTTAGCTTCGATGGTAAGACCGGCTTCTCCCTCAACGGAAAACCTTATAAATTACGCGGTATTTGCCGCCATCAGGACCAAAAACCCATCGGAGTTGCCCTGACAGACGAAATGCACCGTCGTGACATGAAGTTAATGAAAGAGATGGGTGCCAACTTCATCCGCATCTCCCATTATCCGCAAGATGACGCTCTGTTGGAAATGTGCGACAAACTCGGTATGCTAGCTTGGGAAGAAATACCCATTATTGATATTGTGCCCGACACTCCCGGCTATGCAGAAAACTGCGAAAACAATCTGCGAGAAATGATACGCCAGCACTATAACCATCCCTCCATCATCACCTGGGGATACATGAATGAGATATTACTCGTCACCCAGCGCCGCTATAAAAAAGAAGAAGAACTGAAACCCGTGCTGGAACGGACATTGTCACTGGCCAATCGTTTGGAGAAAGTTCTGAAAGAAGAAGATTCCACTCGTGTCAGCACTATGGCTTTTCATGGCAGTAACAGCTACAATGAGGTAGGACTGGGCAACATCACCGATATTGTCGGTTGGAATCTTTACCAAGGCTGGTATGGAGGAGACCTCACAGGCTTCGAACGTTTCCTGACAGAGCAACACAAAAAACACCCTTCCCATCCGATGATTGTCAGCGAATACGGTGCCGGATCTGACAAGCGTCTACATTCGCTGCAACCGCACGCTTTTGATTTCAGTATCGAGTACCAGCAGAAATACCTGGAACACTACTTACCGGTATTGGAAGAAACGCCGTATATTTGTGGTGGAACTCACTGGAACTTTATCGATTTCTCTTCTGCATTGCGTGATGAATCTATGCCGCGCATCAACAATAAAGGACTGGTATACTCAGACCGCAGTCCAAAAGACGTGTACTATTACTATAAAGCCGCCTGGCGTCAAGACATTCCCGTGTTACACATTGCCAGCCGTGACTGGACGCACCGTAGCGGTGTACAACATGGAAAGGCTCCGGTTCCGCTACCCGTCAAGGTATATACCAATCTTCCGGAAGTGGAACTCTTCATTGACGGAAAATCCTTAGGTAAACAGAAAACAGAGAATTATACAGTTACATTTCAGGTACCATTCAGCCGGAAAGAGCACTTTATCTCCGCCCAAGCTGAAAACAAAGAGTCAGATCAAAGCATCTCCATGATAGAAGATGCCCTCCACATCAACTTCACCCCTATCCCTGCAAATCTGAATGAAACAAACTTACGGAATCTGGAACTTGCCGTAAATGTAGGCAGCAACTGTTTCTATACTTCCGATGAAAGCCAGCTCACGTGGCTACCCGATCAGCCCTACACTGAAAGCAGTTGGGGATATATCGGCGGAGAAAGCAAAAATAGCCAGACACAGGTTGAGAATACCCATGACGGGCCATTGTTTCAAACTCTTCGCAACGAAATTGAAGGGTATTGTTTCGATGTTCCCAACGGAGTCTACAAGGTAGAATTTCTTTTTACAGATATCTTCCGCGAAAATGCAGCAGCTGTTTACCAGTTGGACCGGGAATCCGGTGTAGAGAGCCGTGAAAATATCTTCGATATAGTTATCAATGGTGAGAGCGTTGAAGAGAACTTTTCTCCTTGCCGCGAAAGCGGTTATTTCCATGCTCTACGGAAAAGATACAATATTACAAACTTTCGGAATAAAATTGAAATACGCTTC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,716 total letters Query= APC115027.107 Length=2928 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4545 0.0 2_04G_G4_APC115027.107_T7S.seq 251 2e-69 1_01G_G4_APC115027.107_T7term.seq 201 3e-54 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W S Query_1 61 CCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCT 150 2 4 ......N...................................................N............................... 93 H P Q F E K G T E N L Y F Q S N A H E S K T I N D S W K F Y Query_1 151 CATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCATGAGAGCAAAACGATCAACGACAGTTGGAAATTCTAT 240 0 1 ....................................... 39 2 94 ..................................................... 146 Q G E C K A A A S A T Y D D S A W N S I H L P H T W N T D A Query_1 241 CAGGGAGAATGCAAAGCTGCCGCTTCAGCAACCTATGATGACAGTGCGTGGAACAGCATACATCTCCCGCATACATGGAATACGGATGCA 330 0 40 .......................................................................................... 129 Y T E K K Y Y Q G T G W Y R R T L T L P Q N W Q T K Q V F L Query_1 331 TATACAGaaaaaaaGTATTACCAGGGAACAGGCTGGTATCGCCGTACGCTGACATTACCGCAAAATTGGCAAACCAAACAGGTATTCCTG 420 0 130 .......................................................................................... 219 K L D A A S K A A A I Y M N G R K I G E H N G G Y T A C T F Query_1 421 AAACTGGATGCAGCCAGTAAGGCCGCTGCCATCTACATGAATGGACGGAAAATAGGTGAGCATAACGGTGGATATACTGCCTGCACATTC 510 0 220 .......................................................................................... 309 D I T P F C S F D A P N S L A I H V D N A R Q D I P P I S A Query_1 511 GATATCACCCCATTCTGTTCATTTGACGCTCCTAATTCTCTCGCTATCCATGTAGATAATGCCCGACAGGATATTCCCCCTATCTCTGCC 600 0 310 .......................................................................................... 399 D F T F F G G I Y R D V W L T A V P K Q H F H L T N Y G S E Query_1 601 GACTTTACATTCTTCGGCGGTATCTACCGGGATGTATGGCTTACAGCCGTACCAAAACAACATTTCCATTTGACGAACTATGGTTCAGAA 690 0 400 .......................................................................................... 489 G I F I S T P Q V S E E K G T I L I R G E I K N D A E Q K A Query_1 691 GGAATATTTATCAGTACCCCGCAAGTATCCGAAGAAAAAGGCACAATACTTATTCGCGGAGAGATAAAGAACGATGCAGAACAGAAAGCA 780 0 490 .......................................................................................... 579 S L E L E H I V Y N P D G S I A Q T Q K Q S I Q I K G G E L Query_1 781 TCTCTTGAACTGGAACATATCGTATACAATCCCGATGGAAGTATTGCCCAGACCCAGAAACAGTCTATCCAGATAAAAGGTGGAGAATTA 870 0 580 .......................................................................................... 669 0 1906 ........ 1913 2 695 ...........CC...C.C.... 673 Y S F R T E T S P I L S P R L W T P E A P H L Y R I E S I L Query_1 871 TACTCTTTCCGCACCGAAACCTCCCCTATTCTGTCCCCCCGATTATGGACACCCGAGGCCCCTCATCTCTACAGAATAGAAAGCATTCTG 960 0 670 .......................................................................................... 759 0 1914 ... 1916 R D K K T K A I L D H S N H H T G F R W F S F D G K T G F S Query_1 961 CGCGATaaaaaaaCAAAAGCCATCCTAGACCACAGTAATCACCATACAGGCTTCCGCTGGTTTAGCTTCGATGGTAAGACCGGCTTCTCC 1050 0 760 .......................................................................................... 849 L N G K P Y K L R G I C R H Q D Q K P I G V A L T D E M H R Query_1 1051 CTCAACGGAAAACCTTATAAATTACGCGGTATTTGCCGCCATCAGGACCAAAAACCCATCGGAGTTGCCCTGACAGACGAAATGCACCGT 1140 0 850 .......................................................................................... 939 R D M K L M K E M G A N F I R I S H Y P Q D D A L L E M C D Query_1 1141 CGTGACATGAAGTTAATGAAAGAGATGGGTGCCAACTTCATCCGCATCTCCCATTATCCGCAAGATGACGCTCTGTTGGAAATGTGCGAC 1230 0 940 .......................................................................................... 1029 K L G M L A W E E I P I I D I V P D T P G Y A E N C E N N L Query_1 1231 AAACTCGGTATGCTAGCTTGGGAAGAAATACCCATTATTGATATTGTGCCCGACACTCCCGGCTATGCAGAAAACTGCGAAAACAATCTG 1320 0 1030 .......................................................................................... 1119 R E M I R Q H Y N H P S I I T W G Y M N Q I L L V T Q R R Y Query_1 1321 CGAGAAATGATACGCCAGCACTATAACCATCCCTCCATCATCACCTGGGGATACATGAATCAGATATTACTCGTCACCCAGCGCCGCTAT 1410 0 1120 ............................................................G............................. 1209 K K E E E L K P V L E R T L S L A N R L E K V L K E E D S T Query_1 1411 AAAAAAGAAGAAGAACTGAAACCCGTGCTGGAACGGACATTGTCACTGGCCAATCGTTTGGAGAAAGTTCTGAAAGAAGAAGATTCCACT 1500 0 1210 .......................................................................................... 1299 0 1213 ........... 1223 0 1282 ........... 1292 R V S T M A F H G S N S Y N E V G L G N I T D I V G W N L Y Query_1 1501 CGTGTCAGCACTATGGCTTTTCATGGCAGTAACAGCTACAATGAGGTAGGACTGGGCAACATCACCGATATTGTCGGTTGGAATCTTTAC 1590 0 1300 .......................................................................................... 1389 Q G W Y G G D L T G F E R F L T E Q H K K H P S H P M I V S Query_1 1591 CAAGGCTGGTATGGAGGAGACCTCACAGGCTTCGAACGTTTCCTGACAGAGCAACACAAAAAACACCCTTCCCATCCGATGATTGTCAGC 1680 0 1390 .......................................................................................... 1479 E Y G A G S D K R L H S L Q P H A F D F S I E Y Q Q K Y L E Query_1 1681 GAATACGGTGCCGGATCTGACAAGCGTCTACATTCGCTGCAACCGCACGCTTTTGATTTCAGTATCGAGTACCAGCAGAAATACCTGGAA 1770 0 1480 .......................................................................................... 1569 H Y L P V L E E T P Y I C G G T H W N F I D F S S A L R D E Query_1 1771 CACTACTTACCGGTATTGGAAGAAACGCCGTATATTTGTGGTGGAACTCACTGGAACTTTATCGATTTCTCTTCTGCATTGCGTGATGAA 1860 0 1570 .......................................................................................... 1659 S M P R I N N K G L V Y S D R S P K D V Y Y Y Y K A A W R Q Query_1 1861 TCTATGCCGCGCATCAACAATAAAGGACTGGTATACTCAGACCGCAGTCCAAAAGACGTGTACTATTACTATAAAGCCGCCTGGCGTCAA 1950 0 1660 .......................................................................................... 1749 D I P V L H I A S R D W T H R S G V Q H G K A P V P L P V K Query_1 1951 GACATTCCCGTGTTACACATTGCCAGCCGTGACTGGACGCACCGTAGCGGTGTACAACATGGAAAGGCTCCGGTTCCGCTACCCGTCAAG 2040 0 1750 .......................................................................................... 1839 1 618 ........... 608 V Y T N L P E V E L F I D G K S L G K Q K T E N Y T V T F Q Query_1 2041 GTATATACCAATCTTCCGGAAGTGGAACTCTTCATTGACGGAAAATCCTTAGGTAAACAGAAAACAGAGAATTATACAGTTACATTTCAG 2130 0 1840 .......................................................................................... 1929 0 662 ........... 672 V P F S R K E H F I S A Q A E N K E S D Q S I S M I E D A L Query_1 2131 GTACCATTCAGCCGGAAAGAGCACTTTATCTCCGCCCAAGCTGAAAACAAAGAGTCAGATCAAAGCATCTCCATGATAGAAGATGCCCTC 2220 0 1930 .......................................................................................... 2019 H I N F T P I P A N L N E T N L R N L E L A V N V G S N C F Query_1 2221 CACATCAACTTCACCCCTATCCCTGCAAATCTGAATGAAACAAACTTACGGAATCTGGAACTTGCCGTAAATGTAGGCAGCAACTGTTTC 2310 0 2020 .......................................................................................... 2109 Y T S D E S Q L T W L P D Q P Y T E S S W G Y I G G E S K N Query_1 2311 TATACTTCCGATGAAAGCCAGCTCACGTGGCTACCCGATCAGCCCTACACTGAAAGCAGTTGGGGATATATCGGCGGAGAAAGCAAAAAT 2400 0 2110 .......................................................................................... 2199 S Q T Q V E N T H D G P L F Q T L R N E I E G Y C F D V P N Query_1 2401 AGCCAGACACAGGTTGAGAATACCCATGACGGGCCATTGTTTCAAACTCTTCGCAACGAAATTGAAGGGTATTGTTTCGATGTTCCCAAC 2490 0 2200 .......................................................................................... 2289 G V Y K V E F L F T D I F R E N A A A V Y Q L D R E S G V E Query_1 2491 GGAGTCTACAAGGTAGAATTTCTTTTTACAGATATCTTCCGCGAAAATGCAGCAGCTGTTTACCAGTTGGACCGGGAATCCGGTGTAGAG 2580 0 2290 .......................................................................................... 2379 S R E N I F D I V I N G E S V E E N F S P C R E S G Y F H A Query_1 2581 AGCCGTGAAAATATCTTCGATATAGTTATCAATGGTGAGAGCGTTGAAGAGAACTTTTCTCCTTGCCGCGAAAGCGGTTATTTCCATGCT 2670 0 2380 .......................................................................................... 2469 L R K R Y N I T N F R N K I E I R F * H W K W I T D P N S S Query_1 2671 CTACGGAAAAGATACAATATTACAAACTTTCGGAATAAAATTGAAATACGCTTCTAACATTGGAAGTGGATAACGGATCCGAATTCGAGC 2760 0 2470 ...................................................... 2523 1 136 .................................... 101 S V D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 2761 TCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAG-CCCGAAAGGAA 2841 1 100 ......................................................................C.......N... 19 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 13579815 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,716 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115027.108 (APC115027.30-870.P(888).E523Q.pMCSG68)
HESKTINDSWKFYQGECKAAASATYDDSAWNSIHLPHTWNTDAYTEKKYYQGTGWYRRTLTLPQNWQTKQVFLKLDAASKAAAIYMNGRKIGEHNGGYTACTFDITPFCSFDAPNSLAIHVDNARQDIPPISADFTFFGGIYRDVWLTAVPKQHFHLTNYGSEGIFISTPQVSEEKGTILIRGEIKNDAEQKASLELEHIVYNPDGSIAQTQKQSIQIKGGELYSFRTETSPILSPRLWTPEAPHLYRIESILRDKKTKAILDHSNHHTGFRWFSFDGKTGFSLNGKPYKLRGICRHQDQKPIGVALTDEMHRRDMKLMKEMGANFIRISHYPQDDALLEMCDKLGMLAWEEIPIIDIVPDTPGYAENCENNLREMIRQHYNHPSIITWGYMNEILLVTQRRYKKEEELKPVLERTLSLANRLEKVLKEEDSTRVSTMAFHGSNSYNEVGLGNITDIVGWNLYQGWYGGDLTGFERFLTEQHKKHPSHPMIVSEYGAGSDKRLHSLQPHAFDFSIEYQQKYLEHYLPVLEETPYICGGTHWNFIDFSSALRDESMPRINNKGLVYSDRSPKDVYYYYKAAWRQDIPVLHIASRDWTHRSGVQHGKAPVPLPVKVYTNLPEVELFIDGKSLGKQKTENYTVTFQVPFSRKEHFISAQAENKESDQSISMIEDALHINFTPIPANLNETNLRNLELAVNVGSNCFYTSDESQLTWLPDQPYTESSWGYIGGESKNSQTQVENTHDGPLFQTLRNEIEGYCFDVPNGVYKVEFLFTDIFRENAAAVYQLDRESGVESRENIFDIVINGESVEENFSPCRESGYFHALRKRYNITNFRNKIEIRF
CATGAGAGCAAAACGATCAACGACAGTTGGAAATTCTATCAGGGAGAATGCAAAGCTGCCGCTTCAGCAACCTATGATGACAGTGCGTGGAACAGCATACATCTCCCGCATACATGGAATACGGATGCATATACAGAAAAAAAGTATTACCAGGGAACAGGCTGGTATCGCCGTACGCTGACATTACCGCAAAATTGGCAAACCAAACAGGTATTCCTGAAACTGGATGCAGCCAGTAAGGCCGCTGCCATCTACATGAATGGACGGAAAATAGGTGAGCATAACGGTGGATATACTGCCTGCACATTCGATATCACCCCATTCTGTTCATTTGACGCTCCTAATTCTCTCGCTATCCATGTAGATAATGCCCGACAGGATATTCCCCCTATCTCTGCCGACTTTACATTCTTCGGCGGTATCTACCGGGATGTATGGCTTACAGCCGTACCAAAACAACATTTCCATTTGACGAACTATGGTTCAGAAGGAATATTTATCAGTACCCCGCAAGTATCCGAAGAAAAAGGCACAATACTTATTCGCGGAGAGATAAAGAACGATGCAGAACAGAAAGCATCTCTTGAACTGGAACATATCGTATACAATCCCGATGGAAGTATTGCCCAGACCCAGAAACAGTCTATCCAGATAAAAGGTGGAGAATTATACTCTTTCCGCACCGAAACCTCCCCTATTCTGTCCCCCCGATTATGGACACCCGAGGCCCCTCATCTCTACAGAATAGAAAGCATTCTGCGCGATAAAAAAACAAAAGCCATCCTAGACCACAGTAATCACCATACAGGCTTCCGCTGGTTTAGCTTCGATGGTAAGACCGGCTTCTCCCTCAACGGAAAACCTTATAAATTACGCGGTATTTGCCGCCATCAGGACCAAAAACCCATCGGAGTTGCCCTGACAGACGAAATGCACCGTCGTGACATGAAGTTAATGAAAGAGATGGGTGCCAACTTCATCCGCATCTCCCATTATCCGCAAGATGACGCTCTGTTGGAAATGTGCGACAAACTCGGTATGCTAGCTTGGGAAGAAATACCCATTATTGATATTGTGCCCGACACTCCCGGCTATGCAGAAAACTGCGAAAACAATCTGCGAGAAATGATACGCCAGCACTATAACCATCCCTCCATCATCACCTGGGGATACATGAATGAGATATTACTCGTCACCCAGCGCCGCTATAAAAAAGAAGAAGAACTGAAACCCGTGCTGGAACGGACATTGTCACTGGCCAATCGTTTGGAGAAAGTTCTGAAAGAAGAAGATTCCACTCGTGTCAGCACTATGGCTTTTCATGGCAGTAACAGCTACAATGAGGTAGGACTGGGCAACATCACCGATATTGTCGGTTGGAATCTTTACCAAGGCTGGTATGGAGGAGACCTCACAGGCTTCGAACGTTTCCTGACAGAGCAACACAAAAAACACCCTTCCCATCCGATGATTGTCAGCGAATACGGTGCCGGATCTGACAAGCGTCTACATTCGCTGCAACCGCACGCTTTTGATTTCAGTATCGAGTACCAGCAGAAATACCTGGAACACTACTTACCGGTATTGGAAGAAACGCCGTATATTTGTGGTGGAACTCACTGGAACTTTATCGATTTCTCTTCTGCATTGCGTGATGAATCTATGCCGCGCATCAACAATAAAGGACTGGTATACTCAGACCGCAGTCCAAAAGACGTGTACTATTACTATAAAGCCGCCTGGCGTCAAGACATTCCCGTGTTACACATTGCCAGCCGTGACTGGACGCACCGTAGCGGTGTACAACATGGAAAGGCTCCGGTTCCGCTACCCGTCAAGGTATATACCAATCTTCCGGAAGTGGAACTCTTCATTGACGGAAAATCCTTAGGTAAACAGAAAACAGAGAATTATACAGTTACATTTCAGGTACCATTCAGCCGGAAAGAGCACTTTATCTCCGCCCAAGCTGAAAACAAAGAGTCAGATCAAAGCATCTCCATGATAGAAGATGCCCTCCACATCAACTTCACCCCTATCCCTGCAAATCTGAATGAAACAAACTTACGGAATCTGGAACTTGCCGTAAATGTAGGCAGCAACTGTTTCTATACTTCCGATGAAAGCCAGCTCACGTGGCTACCCGATCAGCCCTACACTGAAAGCAGTTGGGGATATATCGGCGGAGAAAGCAAAAATAGCCAGACACAGGTTGAGAATACCCATGACGGGCCATTGTTTCAAACTCTTCGCAACGAAATTGAAGGGTATTGTTTCGATGTTCCCAACGGAGTCTACAAGGTAGAATTTCTTTTTACAGATATCTTCCGCGAAAATGCAGCAGCTGTTTACCAGTTGGACCGGGAATCCGGTGTAGAGAGCCGTGAAAATATCTTCGATATAGTTATCAATGGTGAGAGCGTTGAAGAGAACTTTTCTCCTTGCCGCGAAAGCGGTTATTTCCATGCTCTACGGAAAAGATACAATATTACAAACTTTCGGAATAAAATTGAAATACGCTTC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,811 total letters Query= APC115027.108 Length=2928 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4542 0.0 2_04H_H4_APC115027.108_T7S.seq 226 8e-62 1_01H_H4_APC115027.108_T7term.seq 205 3e-55 F V * L * E G D I H M H H H H H H S S G V D L W S H P Q F Query_1 74 ATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCG 163 2 12 ..........-............................................................................... 100 E K G T E N L Y F Q S N A H E S K T I N D S W K F Y Q G E C Query_1 164 AAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCATGAGAGCAAAACGATCAACGACAGTTGGAAATTCTATCAGGGAGAATGCA 253 0 1 .................................................... 52 2 101 ........................................ 140 K A A A S A T Y D D S A W N S I H L P H T W N T D A Y T E K Query_1 254 AAGCTGCCGCTTCAGCAACCTATGATGACAGTGCGTGGAACAGCATACATCTCCCGCATACATGGAATACGGATGCATATACAGaaaaaa 343 0 53 .......................................................................................... 142 K Y Y Q G T G W Y R R T L T L P Q N W Q T K Q V F L K L D A Query_1 344 aGTATTACCAGGGAACAGGCTGGTATCGCCGTACGCTGACATTACCGCAAAATTGGCAAACCAAACAGGTATTCCTGAAACTGGATGCAG 433 0 143 .......................................................................................... 232 A S K A A A I Y M N G R K I G E H N G G Y T A C T F D I T P Query_1 434 CCAGTAAGGCCGCTGCCATCTACATGAATGGACGGAAAATAGGTGAGCATAACGGTGGATATACTGCCTGCACATTCGATATCACCCCAT 523 0 233 .......................................................................................... 322 F C S F D A P N S L A I H V D N A R Q D I P P I S A D F T F Query_1 524 TCTGTTCATTTGACGCTCCTAATTCTCTCGCTATCCATGTAGATAATGCCCGACAGGATATTCCCCCTATCTCTGCCGACTTTACATTCT 613 0 323 .......................................................................................... 412 F G G I Y R D V W L T A V P K Q H F H L T N Y G S E G I F I Query_1 614 TCGGCGGTATCTACCGGGATGTATGGCTTACAGCCGTACCAAAACAACATTTCCATTTGACGAACTATGGTTCAGAAGGAATATTTATCA 703 0 413 .......................................................................................... 502 S T P Q V S E E K G T I L I R G E I K N D A E Q K A S L E L Query_1 704 GTACCCCGCAAGTATCCGAAGAAAAAGGCACAATACTTATTCGCGGAGAGATAAAGAACGATGCAGAACAGAAAGCATCTCTTGAACTGG 793 0 503 .......................................................................................... 592 2 688 .. 687 1 1084 ............ 1073 E H I V Y N P D G S I A Q T Q K Q S I Q I K G G E L Y S F R Query_1 794 AACATATCGTATACAATCCCGATGGAAGTATTGCCCAGACCCAGAAACAGTCTATCCAGATAAAAGGTGGAGAATTATACTCTTTCCGCA 883 0 593 .......................................................................................... 682 0 1906 ........... 1916 2 686 .........CC...C.C.... 666 T E T S P I L S P R L W T P E A P H L Y R I E S I L R D K K Query_1 884 CCGAAACCTCCCCTATTCTGTCCCCCCGATTATGGACACCCGAGGCCCCTCATCTCTACAGAATAGAAAGCATTCTGCGCGATaaaaaaa 973 0 683 .......................................................................................... 772 T K A I L D H S N H H T G F R W F S F D G K T G F S L N G K Query_1 974 CAAAAGCCATCCTAGACCACAGTAATCACCATACAGGCTTCCGCTGGTTTAGCTTCGATGGTAAGACCGGCTTCTCCCTCAACGGAAAAC 1063 0 773 .......................................................................................... 862 P Y K L R G I C R H Q D Q K P I G V A L T D E M H R R D M K Query_1 1064 CTTATAAATTACGCGGTATTTGCCGCCATCAGGACCAAAAACCCATCGGAGTTGCCCTGACAGACGAAATGCACCGTCGTGACATGAAGT 1153 0 863 .......................................................................................... 952 L M K E M G A N F I R I S H Y P Q D D A L L E M C D K L G M Query_1 1154 TAATGAAAGAGATGGGTGCCAACTTCATCCGCATCTCCCATTATCCGCAAGATGACGCTCTGTTGGAAATGTGCGACAAACTCGGTATGC 1243 0 953 .......................................................................................... 1042 L A W E E I P I I D I V P D T P G Y A E N C E N N L R E M I Query_1 1244 TAGCTTGGGAAGAAATACCCATTATTGATATTGTGCCCGACACTCCCGGCTATGCAGAAAACTGCGAAAACAATCTGCGAGAAATGATAC 1333 0 1043 .......................................................................................... 1132 R Q H Y N H P S I I T W G Y M N E I L L V T Q R R Y K K E E Query_1 1334 GCCAGCACTATAACCATCCCTCCATCATCACCTGGGGATACATGAATGAGATATTACTCGTCACCCAGCGCCGCTATAAAAAAGAAGAAG 1423 0 1133 .......................................................................................... 1222 0 1282 .......... 1291 E L K P V L E R T L S L A N R L E K V L K E E D S T R V S T Query_1 1424 AACTGAAACCCGTGCTGGAACGGACATTGTCACTGGCCAATCGTTTGGAGAAAGTTCTGAAAGAAGAAGATTCCACTCGTGTCAGCACTA 1513 0 1223 .......................................................................................... 1312 0 1213 ........... 1223 0 1292 . 1292 M A F H G S N S Y N E V G L G N I T D I V G W N L Y Q G W Y Query_1 1514 TGGCTTTTCATGGCAGTAACAGCTACAATGAGGTAGGACTGGGCAACATCACCGATATTGTCGGTTGGAATCTTTACCAAGGCTGGTATG 1603 0 1313 .......................................................................................... 1402 G G D L T G F E R F L T E Q H K K H P S H P M I V S Q Y G A Query_1 1604 GAGGAGACCTCACAGGCTTCGAACGTTTCCTGACAGAGCAACACAAAAAACACCCTTCCCATCCGATGATTGTCAGCCAGTACGGTGCCG 1693 0 1403 .............................................................................G.A.......... 1492 G S D K R L H S L Q P H A F D F S I E Y Q Q K Y L E H Y L P Query_1 1694 GATCTGACAAGCGTCTACATTCGCTGCAACCGCACGCTTTTGATTTCAGTATCGAGTACCAGCAGAAATACCTGGAACACTACTTACCGG 1783 0 1493 .......................................................................................... 1582 V L E E T P Y I C G G T H W N F I D F S S A L R D E S M P R Query_1 1784 TATTGGAAGAAACGCCGTATATTTGTGGTGGAACTCACTGGAACTTTATCGATTTCTCTTCTGCATTGCGTGATGAATCTATGCCGCGCA 1873 0 1583 .......................................................................................... 1672 I N N K G L V Y S D R S P K D V Y Y Y Y K A A W R Q D I P V Query_1 1874 TCAACAATAAAGGACTGGTATACTCAGACCGCAGTCCAAAAGACGTGTACTATTACTATAAAGCCGCCTGGCGTCAAGACATTCCCGTGT 1963 0 1673 .......................................................................................... 1762 L H I A S R D W T H R S G V Q H G K A P V P L P V K V Y T N Query_1 1964 TACACATTGCCAGCCGTGACTGGACGCACCGTAGCGGTGTACAACATGGAAAGGCTCCGGTTCCGCTACCCGTCAAGGTATATACCAATC 2053 0 1763 .......................................................................................... 1852 1 615 ........... 605 L P E V E L F I D G K S L G K Q K T E N Y T V T F Q V P F S Query_1 2054 TTCCGGAAGTGGAACTCTTCATTGACGGAAAATCCTTAGGTAAACAGAAAACAGAGAATTATACAGTTACATTTCAGGTACCATTCAGCC 2143 0 1853 .......................................................................................... 1942 0 662 ........... 672 R K E H F I S A Q A E N K E S D Q S I S M I E D A L H I N F Query_1 2144 GGAAAGAGCACTTTATCTCCGCCCAAGCTGAAAACAAAGAGTCAGATCAAAGCATCTCCATGATAGAAGATGCCCTCCACATCAACTTCA 2233 0 1943 .......................................................................................... 2032 T P I P A N L N E T N L R N L E L A V N V G S N C F Y T S D Query_1 2234 CCCCTATCCCTGCAAATCTGAATGAAACAAACTTACGGAATCTGGAACTTGCCGTAAATGTAGGCAGCAACTGTTTCTATACTTCCGATG 2323 0 2033 .......................................................................................... 2122 E S Q L T W L P D Q P Y T E S S W G Y I G G E S K N S Q T Q Query_1 2324 AAAGCCAGCTCACGTGGCTACCCGATCAGCCCTACACTGAAAGCAGTTGGGGATATATCGGCGGAGAAAGCAAAAATAGCCAGACACAGG 2413 0 2123 .......................................................................................... 2212 V E N T H D G P L F Q T L R N E I E G Y C F D V P N G V Y K Query_1 2414 TTGAGAATACCCATGACGGGCCATTGTTTCAAACTCTTCGCAACGAAATTGAAGGGTATTGTTTCGATGTTCCCAACGGAGTCTACAAGG 2503 0 2213 .......................................................................................... 2302 V E F L F T D I F R E N A A A V Y Q L D R E S G V E S R E N Query_1 2504 TAGAATTTCTTTTTACAGATATCTTCCGCGAAAATGCAGCAGCTGTTTACCAGTTGGACCGGGAATCCGGTGTAGAGAGCCGTGAAAATA 2593 0 2303 .......................................................................................... 2392 I F D I V I N G E S V E E N F S P C R E S G Y F H A L R K R Query_1 2594 TCTTCGATATAGTTATCAATGGTGAGAGCGTTGAAGAGAACTTTTCTCCTTGCCGCGAAAGCGGTTATTTCCATGCTCTACGGAAAAGAT 2683 0 2393 .......................................................................................... 2482 Y N I T N F R N K I E I R F * H W K W I T D P N S S S V D K Query_1 2684 ACAATATTACAAACTTTCGGAATAAAATTGAAATACGCTTCTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGC 2773 0 2483 ......................................... 2523 1 133 ................................................. 85 L A A A L E H H H H H H * D P A A N K A R Query_1 2774 TTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAA 2837 1 84 ................................................................ 21 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 13856360 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,811 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115038.151 (APC115038.26-783.P(785).H214A.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVTGDITTGQAKSSDVAQKIEKGLVGGLFNLKGVDRILEVQKLAVEKSRLGIPLLFGMDVIHGYETIFPIPLGLSCTWDMAAIEKSARIAAIEASADGISWTFSPMVDISRDPRWGRVSEGSGEDPFLGGAIAQAMVYGYQGANLQDQLHRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEFMYPYEAAVEAGVGSVMASFNEIDGIPATGNKWLLSDLLRGQWGFEGFVVTDFTGISEMIEHGVGDLQTVSALALNAGVDMDMVSEGFVGTLMKSIKEGKVRMGTLNTACRRILEAKYKLGLFDNPYKYCDVNRPKRDIFTKEHRDAARKIAGESFVLLKNAPATAQPLAAHSSSPVTASPVLPLKKQGTVAVIGPLGNTRSNMPGTWSVAARLNDYPSLYEGLKEMMAGKVNITYAKGSNLIGDAAYEERATMFGRSLNRDNRTDQELLDEALKIAAGADVIVAALGESSEMSGESSSRTELGLPDVQHTLLEALLKTGKPVVLTLFTGRPLTLNWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLAAGKWFEKFRSNYLDVDNEPLYPFGYGLSYTTFQYSDIALSTPTLGKDGSVTAVVTVTNTGKHDGAEVVQLYIRDLVGSITRPVRELKGFNKIFLRAGESKTVSFTITRDLLRFYDYDLNYVAEPGDFDIMIGGNSQAVKTAKLTL
AAGTCACCGCAAGACATGGATCGCTTCATCGACGCACTGATGAAGAAGATGACCGTGGAAGAGAAAATCGGACAATTGAACCTACCCGTCACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCACAAAAGATTGAAAAAGGATTGGTGGGCGGACTCTTCAACCTAAAAGGTGTAGACCGTATTCTTGAAGTGCAAAAGCTGGCAGTAGAGAAATCACGCCTCGGTATTCCCCTGCTGTTCGGCATGGATGTGATACATGGCTACGAAACCATCTTCCCCATTCCATTGGGATTGTCCTGCACCTGGGATATGGCGGCTATCGAGAAATCCGCCCGTATTGCAGCCATCGAAGCAAGTGCCGATGGCATTTCCTGGACATTCAGTCCGATGGTAGACATCAGTCGCGACCCACGTTGGGGACGTGTCAGCGAGGGCTCGGGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTATACGGATACCAGGGTGCCAATCTGCAAGACCAATTGCACCGCAACGATGAAATCATGGCTTGCGTAAAACACTTTGCATTGTATGGAGCCGGAGAAGCCGGACGCGACTATAATACAGTAGATATGAGCCGCAACCGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAGGCCGGAGTGGGTAGTGTGATGGCGTCATTCAATGAAATAGACGGTATTCCCGCCACCGGAAACAAATGGCTGCTGAGCGATTTGCTGCGTGGCCAGTGGGGCTTCGAAGGGTTTGTGGTAACGGACTTCACAGGCATTTCAGAGATGATAGAGCATGGTGTCGGCGACTTGCAAACCGTCAGTGCACTCGCTCTTAATGCAGGGGTGGACATGGATATGGTAAGTGAGGGCTTCGTCGGTACACTGATGAAATCAATTAAAGAAGGAAAAGTAAGAATGGGCACGTTGAATACAGCCTGCCGCCGGATATTGGAAGCGAAATACAAGCTGGGACTGTTTGACAATCCTTATAAATACTGCGACGTGAACCGTCCGAAGCGGGATATCTTCACAAAAGAGCATCGTGACGCCGCCCGCAAGATTGCCGGCGAAAGTTTTGTTCTTCTGAAGAATGCCCCCGCCACCGCACAGCCACTCGCAGCTCATAGCTCGTCACCCGTAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAAGGTACAGTTGCCGTCATCGGCCCTCTCGGAAATACCCGCAGCAACATGCCGGGCACCTGGAGCGTAGCCGCACGCCTCAACGATTATCCTTCTTTATACGAAGGCTTGAAAGAAATGATGGCAGGCAAGGTGAACATCACCTATGCCAAAGGTAGTAACCTCATCGGCGATGCAGCTTACGAAGAACGTGCCACCATGTTCGGCCGTTCATTGAACCGCGATAATCGCACGGACCAGGAGTTACTGGACGAAGCACTGAAAATTGCAGCCGGCGCCGATGTTATCGTAGCTGCCCTGGGAGAATCTTCTGAAATGAGCGGTGAAAGTTCAAGCCGCACCGAACTCGGCTTGCCCGATGTACAACATACCCTGTTGGAAGCCTTACTGAAAACGGGTAAACCCGTAGTACTAACCCTCTTTACCGGTCGGCCGTTGACGCTGAACTGGGAACAGGAGCATGTACCTGCCATCTTGAATGTATGGTTCGGAGGCAGTGAAGCGGCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTCAATCCGAGTGGAAAACTAACCATGACTTTCCCGAAGAATGTAGGCCAGATACCTTTGTTCTACAATCATAAGAATACCGGTCGGCCACTGGCGGCAGGCAAATGGTTCGAAAAGTTCCGTTCAAACTATCTGGATGTGGATAACGAACCGCTGTATCCCTTCGGTTATGGATTGTCGTATACCACTTTCCAGTACAGTGACATTGCATTGAGCACACCGACATTGGGAAAAGATGGTTCCGTTACAGCCGTAGTCACCGTCACCAATACTGGTAAACATGACGGTGCGGAAGTAGTTCAACTCTATATCCGCGACCTCGTAGGAAGTATCACCCGCCCTGTACGCGAGTTGAAAGGTTTCAATAAAATCTTCCTTCGCGCCGGAGAAAGCAAAACGGTATCATTCACTATCACGCGTGATCTGCTTCGCTTCTATGATTACGACCTGAACTACGTAGCCGAACCGGGTGACTTTGACATCATGATCGGTGGAAACAGCCAGGCTGTGAAGACGGCGAAGTTGACACTT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,731 total letters Query= APC115038.151 Length=2679 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4087 0.0 1_01A_APC115038.151Seq171A01.seq 1842 0.0 2_A1_APC115038.151Seq171A07.seq 1490 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATT-GTGAGCGGATAACAA-TTCCCCTCTAGAAATAATTTTGTTTAACTTT 88 2 847 ..........................................-...............A............................... 759 2 779 ...G.........T..T.T.C.C.C....-.......A... 818 * E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E Query_1 89 AAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGA 178 2 758 .............N............................................................................ 669 N L Y F Q S N A K S P Q D M D R F I D A L M K K M T V E E K Query_1 179 ACCTGTACTTCCAATCCAATGCCAAGTCACCGCAAGACATGGATCGCTTCATCGACGCACTGATGAAGAAGATGACCGTGGAAGAGAAAA 268 0 1 ................................................................... 67 2 668 .......................................................................................... 579 I G Q L N L P V T G D I T T G Q A K S S D V A Q K I E K G L Query_1 269 TCGGACAATTGAACCTACCCGTCACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCACAAAAGATTGAAAAAGGATTGG 358 0 68 .......................................................................................... 157 2 578 .......................................................................................... 489 V G G L F N L K G V D R I L E V Q K L A V E K S R L G I P L Query_1 359 TGGGCGGACTCTTCAACCTAAAAGGTGTAGACCGTATTCTTGAAGTGCAAAAGCTGGCAGTAGAGAAATCACGCCTCGGTATTCCCCTGC 448 0 158 .......................................................................................... 247 2 488 .......................................................................................... 399 L F G M D V I H G Y E T I F P I P L G L S C T W D M A A I E Query_1 449 TGTTCGGCATGGATGTGATACATGGCTACGAAACCATCTTCCCCATTCCATTGGGATTGTCCTGCACCTGGGATATGGCGGCTATCGAGA 538 0 248 .......................................................................................... 337 2 398 .......................................................................................... 309 K S A R I A A I E A S A D G I S W T F S P M V D I S R D P R Query_1 539 AATCCGCCCGTATTGCAGCCATCGAAGCAAGTGCCGATGGCATTTCCTGGACATTCAGTCCGATGGTAGACATCAGTCGCGACCCACGTT 628 0 338 .......................................................................................... 427 2 308 .......................................................................................... 219 W G R V S E G S G E D P F L G G A I A Q A M V Y G Y Q G A N Query_1 629 GGGGACGTGTCAGCGAGGGCTCGGGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTATACGGATACCAGGGTGCCAATC 718 0 428 .......................................................................................... 517 1 11 ............................ 38 2 218 .......................................................................................... 129 L Q D Q L H R N D E I M A C V K A F A L Y G A G E A G R D Y Query_1 719 TGCAAGACCAATTGCACCGCAACGATGAAATCATGGCTTGCGTAAAAGCTTTTGCATTGTATGGAGCCGGAGAAGCCGGACGCGACTATA 808 0 518 ...............................................CAC........................................ 607 1 39 .......................................................................................... 128 2 128 .......................................................................................... 39 N T V D M S R N R M F N E F M Y P Y E A A V E A G V G S V M Query_1 809 ATACAGTAGATATGAGCCGC-AACCGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAGGCCGGAGTGGGTAGTGTGATG 897 0 608 ....................-..................................................................... 696 1 129 ....................-..................................................................... 217 2 38 ....................A......GAN..... 4 A S F N E I D G I P A T G N K W L L S D L L R G Q W G F E G Query_1 898 GCGTCATTCAATGAAATAGACGGTATTCCCGCCACCGGAAACAAATGGCTGCTGAGCGATTTGCTGCGTGGCCAGTGGGGCTTCGAAGGG 987 0 697 .......................................................................................... 786 1 218 .......................................................................................... 307 F V V T D F T G I S E M I E H G V G D L Q T V S A L A L N A Query_1 988 TTTGTGGTAACGGACTTCACAGGCATTTCAGAGATGATAGAGCATGGTGTCGGCGACTTGCAAACCGTCAGTGCACTCGCTCTTAATGCA 1077 0 787 .......................................................................................... 876 1 308 .......................................................................................... 397 G V D M D M V S E G F V G T L M K S I K E G K V R M G T L N Query_1 1078 GGGGTGGACATGGATATGGTAAGTGAGGGCTTCGTCGGTACACTGATGAAATCAATTAAAGAAGGAAAAGTAAGAATGGGCACGTTGAAT 1167 0 877 .......................................................................................... 966 1 398 .......................................................................................... 487 T A C R R I L E A K Y K L G L F D N P Y K Y C D V N R P K R Query_1 1168 ACAGCCTGCCGCCGGATATTGGAAGCGAAATACAAGCTGGGACTGTTTGACAATCCTTATAAATACTGCGACGTGAACCGTCCGAAGCGG 1257 0 967 .......................................................................................... 1056 1 488 .......................................................................................... 577 D I F T K E H R D A A R K I A G E S F V L L K N A P A T A Q Query_1 1258 GATATCTTCACAAAAGAGCATCGTGACGCCGCCCGCAAGATTGCCGGCGAAAGTTTTGTTCTTCTGAAGAATGCCCCCGCCACCGCACAG 1347 0 1057 .......................................................................................... 1146 1 578 .......................................................................................... 667 P L A A H S S S P V T A S P V L P L K K Q G T V A V I G P Query_1 1348 CCACTCGCAGCTCATAGCTCGTCACCCGTAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAA-GGTACAGTTGCCGTCATCGGCCCTCT 1436 0 1147 ...............................................................-.......................... 1235 1 668 ...............................................................N.......................... 757 L G N T R S N M P G T W S V A A R L N D Y P S L Y E G L K E Query_1 1437 CGGAAATACCCGCAGCAACATGCCGGGCACCTGGAGCGTAGCCGCACGCCTCAACGATTATCCTTCTTTATACGAAGGCTTGAAAGAAAT 1526 0 1236 .......................................................................................... 1325 1 758 .......................................................................................... 847 2 888 ........... 878 M M A G K V N I T Y A K G S N L I G D A A Y E E R A T M F G Query_1 1527 GATGGCAGGCAAGGTGAACATCACCTATGCCAAAGGTAGTAACCTCATCGGCGATGCAGCTTACGAAGAACGTGCCACCATGTTCGGCCG 1616 0 1326 .......................................................................................... 1415 1 848 ...........................................................N.......-N..................-N. 935 R S L N R D N R T D Q E L L D E A L K I A A G A D V I V A A Query_1 1617 TTCATTGAACCGCGATAATCGCACGGACCAGGAGTTACTGGACGAAGCACTGAAAATTGCAGCCGGCGCCGATGTTATCGTAGCTGCCCT 1706 0 1416 .......................................................................................... 1505 1 936 ................-.............-.N................................N..............N.-.....TG 1022 L G E S S E M S G E S S S R T E L G L P D V Q H T L L E A Query_1 1707 GGGAGAATCTTCTGAAATG-AGCGGTGAAAGTTCAAGCCGCACCGAACTC-GGCTTGCCCGATGTACAACATACCCTGTTGG-AAGCCTT 1793 0 1506 ...................-..............................-...............................-....... 1592 1 1023 .................N.A...T.AN.......................NN....-N.....N...-.N..NN.T..GN..T....... 1110 L L K T G K P V V L T L F T G R P L T L N W E Q E H V P A I Query_1 1794 ACTGAAAACGGGTAAACCCGTAGTACTAACCCTCTTTACCGGTCGGCCGTTGACGCTGAACTGGGAACAGGAGCATGTACCTGCCATCTT 1883 0 1593 .......................................................................................... 1682 0 1821 ............ 1832 1 1111 .....N.CG......N.. 1128 L N V W F G G S E A A Y A I G D V L F G D V N P S G K L T M Query_1 1884 GAATGTATGGTTCGGAGGCAGTGAAGCGGCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTCAATCCGAGTGGAAAACTAACCATGAC 1973 0 1683 .......................................................................................... 1772 T F P K N V G Q I P L F Y N H K N T G R P L A A G K W F E K Query_1 1974 TTTCCCGAAGAATGTAGGCCAGATACCTTTGTTCTACAATCATAAGAATACCGGTCGGCCACTGGCGGCAGGCAAATGGTTCGAAAAGTT 2063 0 1773 .......................................................................................... 1862 0 1629 ............ 1640 F R S N Y L D V D N E P L Y P F G Y G L S Y T T F Q Y S D I Query_1 2064 CCGTTCAAACTATCTGGATGTGGATAACGAACCGCTGTATCCCTTCGGTTATGGATTGTCGTATACCACTTTCCAGTACAGTGACATTGC 2153 0 1863 .......................................................................................... 1952 A L S T P T L G K D G S V T A V V T V T N T G K H D G A E V Query_1 2154 ATTGAGCACACCGACATTGGGAAAAGATGGTTCCGTTACAGCCGTAGTCACCGTCACCAATACTGGTAAACATGACGGTGCGGAAGTAGT 2243 0 1953 .......................................................................................... 2042 V Q L Y I R D L V G S I T R P V R E L K G F N K I F L R A G Query_1 2244 TCAACTCTATATCCGCGACCTCGTAGGAAGTATCACCCGCCCTGTACGCGAGTTGAAAGGTTTCAATAAAATCTTCCTTCGCGCCGGAGA 2333 0 2043 .......................................................................................... 2132 E S K T V S F T I T R D L L R F Y D Y D L N Y V A E P G D F Query_1 2334 AAGCAAAACGGTATCATTCACTATCACGCGTGATCTGCTTCGCTTCTATGATTACGACCTGAACTACGTAGCCGAACCGGGTGACTTTGA 2423 0 2133 .......................................................................................... 2222 D I M I G G N S Q A V K T A K L T L Query_1 2424 CATCATGATCGGTGGAAACAGCCAGGCTGTGAAGACGGCGAAGTTGACACTT 2475 0 2223 .................................................... 2274 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12458160 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,731 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115038.152 (APC115038.26-783.P(785).F646A.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVTGDITTGQAKSSDVAQKIEKGLVGGLFNLKGVDRILEVQKLAVEKSRLGIPLLFGMDVIHGYETIFPIPLGLSCTWDMAAIEKSARIAAIEASADGISWTFSPMVDISRDPRWGRVSEGSGEDPFLGGAIAQAMVYGYQGANLQDQLHRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEFMYPYEAAVEAGVGSVMASFNEIDGIPATGNKWLLSDLLRGQWGFEGFVVTDFTGISEMIEHGVGDLQTVSALALNAGVDMDMVSEGFVGTLMKSIKEGKVRMGTLNTACRRILEAKYKLGLFDNPYKYCDVNRPKRDIFTKEHRDAARKIAGESFVLLKNAPATAQPLAAHSSSPVTASPVLPLKKQGTVAVIGPLGNTRSNMPGTWSVAARLNDYPSLYEGLKEMMAGKVNITYAKGSNLIGDAAYEERATMFGRSLNRDNRTDQELLDEALKIAAGADVIVAALGESSEMSGESSSRTELGLPDVQHTLLEALLKTGKPVVLTLFTGRPLTLNWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLAAGKWFEKFRSNYLDVDNEPLYPFGYGLSYTTFQYSDIALSTPTLGKDGSVTAVVTVTNTGKHDGAEVVQLYIRDLVGSITRPVRELKGFNKIFLRAGESKTVSFTITRDLLRFYDYDLNYVAEPGDFDIMIGGNSQAVKTAKLTL
AAGTCACCGCAAGACATGGATCGCTTCATCGACGCACTGATGAAGAAGATGACCGTGGAAGAGAAAATCGGACAATTGAACCTACCCGTCACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCACAAAAGATTGAAAAAGGATTGGTGGGCGGACTCTTCAACCTAAAAGGTGTAGACCGTATTCTTGAAGTGCAAAAGCTGGCAGTAGAGAAATCACGCCTCGGTATTCCCCTGCTGTTCGGCATGGATGTGATACATGGCTACGAAACCATCTTCCCCATTCCATTGGGATTGTCCTGCACCTGGGATATGGCGGCTATCGAGAAATCCGCCCGTATTGCAGCCATCGAAGCAAGTGCCGATGGCATTTCCTGGACATTCAGTCCGATGGTAGACATCAGTCGCGACCCACGTTGGGGACGTGTCAGCGAGGGCTCGGGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTATACGGATACCAGGGTGCCAATCTGCAAGACCAATTGCACCGCAACGATGAAATCATGGCTTGCGTAAAACACTTTGCATTGTATGGAGCCGGAGAAGCCGGACGCGACTATAATACAGTAGATATGAGCCGCAACCGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAGGCCGGAGTGGGTAGTGTGATGGCGTCATTCAATGAAATAGACGGTATTCCCGCCACCGGAAACAAATGGCTGCTGAGCGATTTGCTGCGTGGCCAGTGGGGCTTCGAAGGGTTTGTGGTAACGGACTTCACAGGCATTTCAGAGATGATAGAGCATGGTGTCGGCGACTTGCAAACCGTCAGTGCACTCGCTCTTAATGCAGGGGTGGACATGGATATGGTAAGTGAGGGCTTCGTCGGTACACTGATGAAATCAATTAAAGAAGGAAAAGTAAGAATGGGCACGTTGAATACAGCCTGCCGCCGGATATTGGAAGCGAAATACAAGCTGGGACTGTTTGACAATCCTTATAAATACTGCGACGTGAACCGTCCGAAGCGGGATATCTTCACAAAAGAGCATCGTGACGCCGCCCGCAAGATTGCCGGCGAAAGTTTTGTTCTTCTGAAGAATGCCCCCGCCACCGCACAGCCACTCGCAGCTCATAGCTCGTCACCCGTAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAAGGTACAGTTGCCGTCATCGGCCCTCTCGGAAATACCCGCAGCAACATGCCGGGCACCTGGAGCGTAGCCGCACGCCTCAACGATTATCCTTCTTTATACGAAGGCTTGAAAGAAATGATGGCAGGCAAGGTGAACATCACCTATGCCAAAGGTAGTAACCTCATCGGCGATGCAGCTTACGAAGAACGTGCCACCATGTTCGGCCGTTCATTGAACCGCGATAATCGCACGGACCAGGAGTTACTGGACGAAGCACTGAAAATTGCAGCCGGCGCCGATGTTATCGTAGCTGCCCTGGGAGAATCTTCTGAAATGAGCGGTGAAAGTTCAAGCCGCACCGAACTCGGCTTGCCCGATGTACAACATACCCTGTTGGAAGCCTTACTGAAAACGGGTAAACCCGTAGTACTAACCCTCTTTACCGGTCGGCCGTTGACGCTGAACTGGGAACAGGAGCATGTACCTGCCATCTTGAATGTATGGTTCGGAGGCAGTGAAGCGGCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTCAATCCGAGTGGAAAACTAACCATGACTTTCCCGAAGAATGTAGGCCAGATACCTTTGTTCTACAATCATAAGAATACCGGTCGGCCACTGGCGGCAGGCAAATGGTTCGAAAAGTTCCGTTCAAACTATCTGGATGTGGATAACGAACCGCTGTATCCCTTCGGTTATGGATTGTCGTATACCACTTTCCAGTACAGTGACATTGCATTGAGCACACCGACATTGGGAAAAGATGGTTCCGTTACAGCCGTAGTCACCGTCACCAATACTGGTAAACATGACGGTGCGGAAGTAGTTCAACTCTATATCCGCGACCTCGTAGGAAGTATCACCCGCCCTGTACGCGAGTTGAAAGGTTTCAATAAAATCTTCCTTCGCGCCGGAGAAAGCAAAACGGTATCATTCACTATCACGCGTGATCTGCTTCGCTTCTATGATTACGACCTGAACTACGTAGCCGAACCGGGTGACTTTGACATCATGATCGGTGGAAACAGCCAGGCTGTGAAGACGGCGAAGTTGACACTT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,497 total letters Query= APC115038.152 Length=2679 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4087 0.0 2_10B_B1_APC115038.152_T7term.seq 1817 0.0 1_01B_APC115038.152Seq171B01.seq 1555 0.0 F E K G T E N L Y F Q S N A K S P Q D M D R F I D A L M K Query_1 159 GTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCAAGTCACCGCAAGACATGGATCGCTTCATCGACGCACTGATGAAGAA 248 0 1 ............................................... 47 2 559 ........... 549 1 707 ........... 717 K M T V E E K I G Q L N L P V T G D I T T G Q A K S S D V A Query_1 249 GATGACCGTGGAAGAGAAAATCGGACAATTGAACCTACCCGTCACGGGAGACATCACCACGGGACAGGCCAAAAGTAGCGACGTGGCACA 338 0 48 .......................................................................................... 137 Q K I E K G L V G G L F N L K G V D R I L E V Q K L A V E K Query_1 339 AAAGATTGAAAAAGGATTGGTGGGCGGACTCTTCAACCTAAAAGGTGTAGACCGTATTCTTGAAGTGCAAAAGCTGGCAGTAGAGAAATC 428 0 138 .......................................................................................... 227 S R L G I P L L F G M D V I H G Y E T I F P I P L G L S C T Query_1 429 ACGCCTCGGTATTCCCCTGCTGTTCGGCATGGATGTGATACATGGCTACGAAACCATCTTCCCCATTCCATTGGGATTGTCCTGCACCTG 518 0 228 .......................................................................................... 317 W D M A A I E K S A R I A A I E A S A D G I S W T F S P M V Query_1 519 GGATATGGCGGCTATCGAGAAATCCGCCCGTATTGCAGCCATCGAAGCAAGTGCCGATGGCATTTCCTGGACATTCAGTCCGATGGTAGA 608 0 318 .......................................................................................... 407 D I S R D P R W G R V S E G S G E D P F L G G A I A Q A M V Query_1 609 CATCAGTCGCGACCCACGTTGGGGACGTGTCAGCGAGGGCTCGGGAGAAGATCCGTTTCTGGGTGGAGCTATCGCACAGGCAATGGTATA 698 0 408 .......................................................................................... 497 Y G Y Q G A N L Q D Q L H R N D E I M A C V K H F A L Y G A Query_1 699 CGGATACCAGGGTGCCAATCTGCAAGACCAATTGCACCGCAACGATGAAATCATGGCTTGCGTAAAACACTTTGCATTGTATGGAGCCGG 788 0 498 .......................................................................................... 587 G E A G R D Y N T V D M S R N R M F N E F M Y P Y E A A V E Query_1 789 AGAAGCCGGACGCGACTATAATACAGTAGATATGAGCCGCAACCGGATGTTCAATGAATTCATGTACCCGTATGAGGCTGCCGTAGAGGC 878 0 588 .......................................................................................... 677 A G V G S V M A S F N E I D G I P A T G N K W L L S D L L R Query_1 879 CGGAGTGGGTAGTGTGATGGCGTCATTCAATGAAATAGACGGTATTCCCGCCACCGGAAACAAATGGCTGCTGAGCGATTTGCTGCGTGG 968 0 678 .......................................................................................... 767 G Q W G F E G F V V T D F T G I S E M I E H G V G D L Q T V Query_1 969 CCAGTGGGGCTTCGAAGGGTTTGTGGTAACGGACTTCACAGGCATTTCAGAGATGATAGAGCATGGTGTCGGCGACTTGCAAACCGTCAG 1058 0 768 .......................................................................................... 857 S A L A L N A G V D M D M V S E G F V G T L M K S I K E G K Query_1 1059 TGCACTCGCTCTTAATGCAGGGGTGGACATGGATATGGTAAGTGAGGGCTTCGTCGGTACACTGATGAAATCAATTAAAGAAGGAAAAGT 1148 0 858 .......................................................................................... 947 V R M G T L N T A C R R I L E A K Y K L G L F D N P Y K Y C Query_1 1149 AAGAATGGGCACGTTGAATACAGCCTGCCGCCGGATATTGGAAGCGAAATACAAGCTGGGACTGTTTGACAATCCTTATAAATACTGCGA 1238 0 948 .......................................................................................... 1037 D V N R P K R D I F T K E H R D A A R K I A G E S F V L L K Query_1 1239 CGTGAACCGTCCGAAGCGGGATATCTTCACAAAAGAGCATCGTGACGCCGCCCGCAAGATTGCCGGCGAAAGTTTTGTTCTTCTGAAGAA 1328 0 1038 .......................................................................................... 1127 N A P A T A Q P L A A H S S S P V T A S P V L P L K K Q G T Query_1 1329 TGCCCCCGCCACCGCACAGCCACTCGCAGCTCATAGCTCGTCACCCGTAACTGCTTCCCCCGTGCTTCCGTTGAAGAAACAAGGTACAGT 1418 0 1128 .......................................................................................... 1217 1 21 .....N....G.......................................... 73 V A V I G P L G N T R S N M P G T W S V A A R L N D Y P S L Query_1 1419 TGCCGTCATCGGCCCTCTCGGAAATACCCGCAGCAACATGCCGGGCACCTGGAGCGTAGCCGCACGCCTCAACGATTATCCTTCTTTATA 1508 0 1218 .......................................................................................... 1307 2 1134 ....N.N..T.....TN....T 1113 1 74 ............N.............................................N..N.........N.................. 163 Y E G L K E M M A G K V N I T Y A K G S N L I G D A A Y E Query_1 1509 CGAAGGCTT-GAAAGAAATGATGGCAGGCAA-GGTGAACATCACCTATG-CCAAAGGTAGTAACCTCATCGGCGATGCAGCTTACGAAGA 1595 0 1308 .........-.....................-.................-........................................ 1394 2 1112 N........N..N.......NNT......N.N.......N..-......G.NN.........C.T......................... 1024 1 164 .N.......-.....................-.................-....................N.......GN........N. 250 E R A T M F G R S L N R D N R T D Q E L L D E A L K I A Query_1 1596 A-CGTGCCACC-ATGTTCGGCCGTTCA-TTGAACCGCGATAATCGCACGGA-CCAGGAGTTACTGGACGAA-GCACTGAAAA-TTGCAGC 1679 0 1395 .-.........-...............-.......................-...................-..........-....... 1478 2 1023 .A.........C..N.A..T.......A....N........N.........G...................N..........AN...... 934 1 251 .-.........-........N.....N-.......................-...NN..............-..........-....N.. 334 A G A D V I V A A L G E S S E M S G E S S S R T E L G L P D Query_1 1680 CGGCGCCGATGTT-ATCGTAGCTGCCCTGGGAGAATCTTCTGAAATGAGCGGTGAAAGTTCAAGCCGCACCGAACTCGGCTTGCCCGATG 1768 0 1479 .............-............................................................................ 1567 2 933 .......N.....T............................................................................ 844 1 335 ...N.........-...N..C..................................................................... 423 V Q H T L L E A L L K T G K P V V L T L F T G R P L T L N W Query_1 1769 TACAACATACCCTGTTGGAAGCCTTACTGAAAACGGGTAAACCCGTAGTACTAACCCTCTTTACCGG-TCGGCCGTTGACGCTGAACTGG 1857 0 1568 ...................................................................-...................... 1656 0 1821 ......-...... 1832 2 843 ...................................................................G...................... 754 2 589 ......-...... 578 1 424 ...................................................................-...................... 512 1 677 ......-...... 688 E Q E H V P A I L N V W F G G S E A A Y A I G D V L F G D V Query_1 1858 GAACAGGAGCATGTACCTGCCATCTTGAATGTATGGTTCGGAGGCAGTGAAGCGGCTTATGCCATTGGCGATGTTCTGTTCGGTGACGTC 1947 0 1657 .......................................................................................... 1746 2 753 .......................................................................................... 664 1 513 .......................................................................................... 602 N P S G K L T M T F P K N V G Q I P L F Y N H K N T G R P L Query_1 1948 AATCCGAGTGGAAAACTAACCATGACTTTCCCGAAGAATGTAGGCCAGATACCTTTGTTCTACAATCATAAGAATACCGGTCGGCCACTG 2037 0 1747 .......................................................................................... 1836 0 1629 ............ 1640 2 663 .......................................................................................... 574 1 603 .......................................................................................... 692 1 485 ............ 496 A A G K W F E K A R S N Y L D V D N E P L Y P F G Y G L S Query_1 2038 GCGGCAGGCAAATGGTTCGAAAAGGCTCG-TTCAAACTATCTGGATGTGGATAACGAACCGCTGTATCCCTTCGGTTATGGATTGTCGTA 2126 0 1837 ........................TTC..-............................................................ 1925 2 573 .............................-........................Y................................... 485 1 693 .............................T........................N...................N............... 782 Y T T F Q Y S D I A L S T P T L G K D G S V T A V V T V T Query_1 2127 TACCAC-TTTCCAGTACAGTGACATTGCATTGAGCACACCGACATTGGG-AAAAGATGGTTCCGTTACAGCCGTAGTCACCGTCACC-AA 2213 0 1926 ......-..........................................-.....................................-.. 2012 2 484 ......-..........................................-.....................................-.. 398 1 783 ......T....................................N.....A...............................N...N.A.. 872 N T G K H D G A E V V Q L Y I R D L V G S I T R P V R E L K Query_1 2214 TACTGGTAAACATGACGGTGCGGAAGTAGTTCAACTCTATATCCGCGACCTCGTAGGAAGTATCACCCGCCCTGTACGCGAGTTGAAAGG 2303 0 2013 .......................................................................................... 2102 2 397 .......................................................................................... 308 1 873 ................-......N....N.................N.N....N.....NGN.......N.....N.T..........T. 961 G F N K I F L R A G E S K T V S F T I T R D L L R F Y D Y D Query_1 2304 TTTCAATAAAATCTTCCTTCGCGCCGGAGAAAGCAAAACGGTATCATTCA-CTATCACGCGTGATCTGCTTCGCTTCTATGATTACGACC 2392 0 2103 ..................................................-....................................... 2191 0 2166 ............ 2155 2 307 ..................................................-....................................... 219 2 244 ............ 255 1 962 G....--T....T..T......NN..N......-.....N.G.A...C..C.......N.NG... 1023 L N Y V A E P G D F D I M I G G N S Q A V K T A K L T L * H Query_1 2393 TGAACTACGTAGCCGAACCGGGTGACTTTGACATCATGATCGGTGGAAACAGCCAGGCTGTGAAGACGGCGAAGTTGACACTTTAACATT 2482 0 2192 ................................................................................... 2274 2 218 ........A................................................................................. 129 W K W I T D P N S S S V D K L A A A L E H H H H H H * D P A Query_1 2483 GGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTG 2572 2 128 .......................................................................................... 39 A N K A R K E Query_1 2573 CTAACAAAGCCCGAAAGGAA 2592 2 38 .................... 19 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11835252 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,497 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115043.128 (APC115043.26-768.P(769).W283Y.pMCSG68)
KSGDASMNKFIDKLMDRMTLEEKIGQLNLPSSGDITTGQARSNNIADKIRAGAVGGLFNIKGVEKIQEVQRIAVEESRLKIPLLFGMDVIHGYETVFPIPLGMAATWDMKAIEQSARIAAIEASADGICWTFSPMVDISRDPRWGRVSEGSGEDPFLGGEIAKAMVYGYQGKGDSAYREKTNIMACVKHYALYGAAEAGLDYNTTDMSRIRMFNEYMYPYQAAVDAGAGSVMSSFNEVDGIPATANKWLITDVLRKQWGFGGFVVTDYTGIMEMVNHGIGDMREVSARALSAGVDMDMVSEGYLSTLQQSLKEGKITEKEIDQACRRILEAKYKLGLFDNPYKYCDTERAKTDIYTDEHRSIARRISAESFVLLKNDKQTLPIKKKGKIAVVGPLANTSSNMPGTWSVAVNMEAPATLVEGLKEVAGDKVEIVYAKGSHLMSDAAYEERATLFGRTLYRDKEKRSDIQMLNEALNVAHGADVVVAALGESSEMSGESSSRTDLNIPDVQKTLLEELVKTGKPVVLVLFTGRPLTLTWEDKNVSAILNVWFGGTEAAYAIGDVLFGNVNPGGKLPVTFPQNVGQIPLFYNHKNTGRPLAEGGWFEKFRANYLDVTNEPLYPFGYGLSYAQFDYSDVRLSTDQIDRNGMLTASVTVTNNSECDGDEIVQLYIRDLVGSVTRPVKELKGFEKVTIRAGESKDISFKITPEMLKFYNSDIQFVNEVGEFEVMIGTNSRDVKKATFSL
AAGAGTGGGGATGCGTCGATGAACAAATTTATTGATAAACTGATGGACAGGATGACCTTGGAAGAGAAGATTGGTCAGCTTAATCTTCCCAGCTCGGGAGATATAACCACCGGACAGGCACGCAGCAACAATATTGCAGACAAAATCAGAGCAGGTGCAGTGGGTGGCTTATTCAATATAAAAGGAGTTGAGAAGATACAGGAAGTACAACGTATTGCTGTAGAGGAGAGTCGCCTGAAAATTCCTTTACTCTTTGGCATGGATGTTATTCATGGGTATGAAACTGTTTTCCCTATTCCTTTGGGTATGGCTGCCACATGGGATATGAAGGCTATAGAACAATCTGCTCGTATAGCGGCGATAGAAGCCAGTGCCGATGGCATCTGCTGGACATTTAGTCCGATGGTTGATATCAGCCGTGATCCACGTTGGGGACGTGTATCCGAAGGTAGCGGAGAAGATCCTTTTTTAGGTGGTGAAATTGCTAAGGCGATGGTATATGGCTATCAGGGTAAAGGTGATAGCGCATATCGTGAAAAGACTAATATTATGGCTTGTGTGAAGCACTATGCCTTGTATGGGGCAGCAGAAGCCGGTTTGGACTATAATACAACTGACATGAGCCGTATTCGTATGTTTAATGAATATATGTATCCTTATCAGGCGGCTGTGGATGCGGGTGCCGGCAGTGTCATGTCTTCTTTCAACGAGGTCGATGGAATTCCTGCAACAGCCAACAAATGGTTGATAACTGATGTCCTGCGTAAACAGTGGGGATTCGGTGGTTTTGTCGTTACGGACTATACCGGTATCATGGAAATGGTAAATCATGGTATTGGAGATATGCGAGAAGTCTCTGCCCGTGCTTTGAGTGCAGGAGTGGATATGGATATGGTGAGCGAAGGTTATCTTTCTACACTTCAACAATCATTGAAGGAGGGTAAGATAACAGAGAAAGAGATAGATCAAGCTTGCCGTCGTATTTTGGAGGCAAAATATAAGCTGGGATTATTTGATAATCCTTATAAGTATTGTGATACTGAACGTGCCAAAACGGATATCTACACTGATGAACATCGGAGTATTGCACGCCGGATCTCTGCTGAAAGCTTTGTTCTTTTAAAGAATGATAAACAGACACTGCCTATAAAGAAAAAAGGTAAGATTGCTGTAGTTGGGCCGTTGGCGAATACGAGTTCTAATATGCCCGGAACGTGGAGTGTAGCGGTCAATATGGAAGCTCCAGCTACGCTTGTGGAGGGTTTGAAAGAAGTGGCAGGTGATAAAGTTGAAATTGTGTATGCTAAGGGTAGCCATCTGATGAGTGATGCGGCTTATGAGGAACGTGCAACACTCTTTGGACGTACATTATACCGGGATAAGGAAAAACGTTCCGATATCCAGATGCTGAATGAAGCATTAAATGTTGCTCATGGTGCCGATGTTGTTGTTGCGGCATTAGGTGAATCTTCTGAAATGAGTGGTGAATCGAGTAGTCGAACAGATTTGAATATTCCTGATGTTCAAAAAACATTATTGGAAGAATTAGTGAAAACAGGTAAACCTGTCGTTCTGGTATTATTCACTGGGCGTCCGTTGACCCTGACATGGGAAGACAAAAATGTATCTGCTATTCTGAATGTTTGGTTTGGAGGTACCGAAGCCGCTTATGCTATAGGAGATGTCCTATTCGGAAATGTAAATCCTGGAGGTAAGCTGCCTGTAACATTTCCTCAGAATGTAGGGCAGATTCCTTTATTCTATAACCATAAAAATACTGGACGTCCGCTGGCTGAGGGCGGTTGGTTTGAGAAGTTCCGGGCAAATTATCTGGATGTAACGAATGAACCTCTTTATCCATTTGGCTATGGACTAAGTTATGCACAATTTGATTATAGCGATGTGAGATTAAGTACGGATCAAATAGACCGGAATGGCATGTTAACCGCAAGTGTGACTGTAACCAATAACAGTGAGTGTGATGGAGATGAAATTGTTCAGTTGTATATTCGCGATTTGGTCGGTAGTGTTACTCGTCCGGTGAAAGAATTGAAAGGATTTGAAAAAGTAACAATTAGAGCAGGGGAGTCAAAAGATATTTCTTTTAAGATCACTCCGGAAATGCTTAAGTTCTACAATTCGGATATCCAGTTTGTGAATGAAGTTGGTGAATTCGAAGTAATGATCGGAACGAACAGCAGGGATGTGAAAAAAGCAACGTTTAGCTTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,631 total letters Query= APC115043.128 Length=2634 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4011 0.0 2_C1_APC115043.128Seq171C07.seq 1788 0.0 1_01C_APC115043.128Seq171C01.seq 1786 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * Query_1 1 TCTCG-ATCCCGCGAAATTAATACGACTC-ACTATAGGGGAATTGTGAGCGGA-TAACAATTCCCCT-CTAGAAATAATTTTGTTTAACT 86 2 1112 .....N.......NN..ANT..N......CNN.......A.T.NN....GN..A.............TNN......NTA...TNN..N.. 1023 2 1046 ........... 1056 L * E G D I H M H H H H H H S S G V D L W S H P Q F E K G Query_1 87 TTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGG-TGTAGATC-TGTGGTCTCATCCG-CAGTTCGAAAA-GGGTA 172 2 1022 ...GN..................N............N.NN.NN.....A........C..............N...........A..... 933 T E N L Y F Q S N A K S G D A S M N K F I D K L M D R M T L Query_1 173 CCGAGAACCTGTACTTCCAATCCAATGCCAAGAGTGGGGATGCGTCGATGAACAAATTTATTGATAAACTGATGGACAGGATGACCTTGG 262 0 1 ............................................................. 61 2 932 .................N.-................N..................................................... 844 E E K I G Q L N L P S S G D I T T G Q A R S N N I A D K I R Query_1 263 AAGAGAAGATTGGTCAGCTTAATCTTCCCAGCTCGGGAGATATAACCACCGGACAGGCACGCAGCAACAATATTGCAGACAAAATCAGAG 352 0 62 .......................................................................................... 151 2 843 .......................................................................................... 754 A G A V G G L F N I K G V E K I Q E V Q R I A V E E S R L K Query_1 353 CAGGTGCAGTGGGTGGCTT-ATTCAATATAAAAGGAGTTGAGAAGATACAGGAAGTACAACGTATTGCTGTAGAGGAGAGTCGCCTGAAA 441 0 152 ...................-...................................................................... 240 2 753 ...................T...................................................................... 664 I P L L F G M D V I H G Y E T V F P I P L G M A A T W D M K Query_1 442 ATTCCTTTACTCTTTGGCATGGATGTTATTCATGGGTATGAAACTGTTTTCCCTATTCCTTTGGGTATGGCTGCCACATGGGATATGAAG 531 0 241 .......................................................................................... 330 2 663 .......................................................................................... 574 A I E Q S A R I A A I E A S A D G I C W T F S P M V D I S R Query_1 532 GCTATAGAACAATCTGCTCGTATAGCGGCGATAGAAGCCAGTGCCGATGGCATCTGCTGGACATTTAGTCCGATGGTTGATATCAGCCGT 621 0 331 .......................................................................................... 420 2 573 .......................................................................................... 484 D P R W G R V S E G S G E D P F L G G E I A K A M V Y G Y Q Query_1 622 GATCCACGTTGGGGACGTGTATCCGAAGGTAGCGGAGAAGATCCTTTTTTAGGTGGTGAAATTGCTAAGGCGATGGTATATGGCTATCAG 711 0 421 .......................................................................................... 510 2 483 .......................................................................................... 394 G K G D S A Y R E K T N I M A C V K H Y A L Y G A A E A G L Query_1 712 GGTAAAGGTGATAGCGCATATCGTGAAAAGACTAATATTATGGCTTGTGTGAAGCACTATGCCTTGTATGGGGCAGCAGAAGCCGGTTTG 801 0 511 .......................................................................................... 600 2 393 .......................................................................................... 304 D Y N T T D M S R I R M F N E Y M Y P Y Q A A V D A G A G S Query_1 802 GACTATAATACAACTGACATGAGCCGTATTCGTATGTTTAATGAATATATGTATCCTTATCAGGCGGCTGTGGATGCGGGTGCCGGCAGT 891 0 601 .......................................................................................... 690 2 303 .......................................................................................... 214 V M S S F N E V D G I P A T A N K W L I T D V L R K Q Y G F Query_1 892 GTCATGTCTTCTTTCAACGAGGTCGATGGAATTCCTGCAACAGCCAACAAATGGTTGATAACTGATGTCCTGCGTAAACAGTATGGATTC 981 0 691 ..................................................................................GG...... 780 2 213 .......................................................................................... 124 1 27 ..................................................................... 95 G G F V V T D Y T G I M E M V N H G I G D M R E V S A R A L Query_1 982 GGTGGTTTTGTCGTTACGGACTATACCGGTATCATGGAAATGGTAAATCATGGTATTGGAGATATGCGAGAAGTCTCTGCCCGTGCTTTG 1071 0 781 .......................................................................................... 870 2 123 .......................................................................................... 34 1 96 N......................................................................................... 185 S A G V D M D M V S E G Y L S T L Q Q S L K E G K I T E K E Query_1 1072 AGTGCAGGAGTGGATATGGATATGGTGAGCGAAGGTTATCTTTCTACACTTCAACAATCATTGAAGGAGGGTAAGATAACAGAGAAAGAG 1161 0 871 .......................................................................................... 960 2 33 .........................-N...... 2 1 186 .......................................................................................... 275 I D Q A C R R I L E A K Y K L G L F D N P Y K Y C D T E R A Query_1 1162 ATAGATCAAGCTTGCCGTCGTATTTTGGAGGCAAAATATAAGCTGGGATTATTTGATAATCCTTATAAGTATTGTGATACTGAACGTGCC 1251 0 961 .......................................................................................... 1050 1 276 .......................................................................................... 365 K T D I Y T D E H R S I A R R I S A E S F V L L K N D K Q T Query_1 1252 AAAACGGATATCTACACTGATGAACATCGGAGTATTGCACGCCGGATCTCTGCTGAAAGCTTTGTTCTTTTAAAGAATGATAAACAGACA 1341 0 1051 .......................................................................................... 1140 1 366 .......................................................................................... 455 L P I K K K G K I A V V G P L A N T S S N M P G T W S V A V Query_1 1342 CTGCCTATAAAGAAAAAAGGTAAGATTGCTGTAGTTGGGCCGTTGGCGAATACGAGTTCTAATATGCCCGGAACGTGGAGTGTAGCGGTC 1431 0 1141 .......................................................................................... 1230 1 456 .......................................................................................... 545 N M E A P A T L V E G L K E V A G D K V E I V Y A K G S H L Query_1 1432 AATATGGAAGCTCCAGCTACGCTTGTGGAGGGTTTGAAAGAAGTGGCAGGTGATAAAGTTGAAATTGTGTATGCTAAGGGTAGCCATCTG 1521 0 1231 .......................................................................................... 1320 1 546 .......................................................................................... 635 M S D A A Y E E R A T L F G R T L Y R D K E K R S D I Q M L Query_1 1522 ATGAGTGATGCGGCTTATGAGGAACGTGCAACACTCTTTGGACGTACATTATACCGGGATAAGGAAAAACGTTCCGATATCCAGATGCTG 1611 0 1321 .......................................................................................... 1410 1 636 .......................................................................................... 725 N E A L N V A H G A D V V V A A L G E S S E M S G E S S S R Query_1 1612 AATGAAGCATTAAATGTTGCTCATGGTGCCGATGTTGTTGTTGCGGCATTAGGTGAATCTTCTGAAATGAGTGGTGAATCGAGTAGTCGA 1701 0 1411 .......................................................................................... 1500 1 726 .......................................................................................... 815 T D L N I P D V Q K T L L E E L V K T G K P V V L V L F T G Query_1 1702 ACAGATTTGAATATTCCTGATGTTCAAAAAACATTATTGGAAGAATTAGTGAAAACAGGTAAACCTGTCGTTCTGGTATTATTCACTGGG 1791 0 1501 .......................................................................................... 1590 1 816 .........................-......N..........................-N............................. 903 R P L T L T W E D K N V S A I L N V W F G G T E A A Y A I Query_1 1792 CGTCCGTTGACCC-TGACATGGGAAGACAAAAATGTATC-TGCTATTCTGAATGTTTGGTTTGGAGGTACCGAAGCCGCTTATGCTATAG 1879 0 1591 .............-.........................-.................................................. 1678 1 904 .............T....N.........-..........T.............................N....A...........N... 992 G D V L F G N V N P G G K L P V T F P Q N V G Q I P L F Query_1 1880 GAGATGTCCTATTCGGAAATGTAAATCCTGGAGGTAAGCTGCCTGTAACA-TTTCCTCAGAA-TGTAGGGCAGATTCCTTT--ATTCT-A 1964 0 1679 ..................................................-...........-..................--.....-. 1763 1 993 N......-..N.............T.............N.N.TN..N...T...........T......N.NNT.......TAT....N. 1081 Y N H K N T G R P L A E G G W F E K F R A N Y L D V T N Query_1 1965 TAACCATAAAAATACTGGACGT-CCGC-TGGCT-GA-GGGCGGTTGGTTT-GAGAAG-TTCCGGGCAAATTATCT-GGATGTAACGAATG 2047 0 1764 ......................-....-.....-..-.............-......-.................-.............. 1846 1 1082 ..C..N.T..N...........N.N..T.....N..T.....NNNN.NN.G..N..NT.....NNA..T......N.N....NN...... 1171 E P L Y P F G Y G L S Y A Q F D Y S D V R L S T D Q I D Query_1 2048 AAC---CTCTTTATCCA--TTTGGCT--ATGGACTAAGTTATGCACAATTTGATTATAGCGATGTGAGATTAAGTACGGATCAAATAGAC 2130 0 1847 ...---...........--.......--.............................................................. 1929 1 1172 TNNCTT...........NT...N...TAN....... 1207 R N G M L T A S V T V T N N S E C D G D E I V Q L Y I R D L Query_1 2131 CGGAATGGCATGTTAACCGCAAGTGTGACTGTAACCAATAACAGTGAGTGTGATGGAGATGAAATTGTTCAGTTGTATATTCGCGATTTG 2220 0 1930 .......................................................................................... 2019 V G S V T R P V K E L K G F E K V T I R A G E S K D I S F K Query_1 2221 GTCGGTAGTGTTACTCGTCCGGTGAAAGAATTGAAAGGATTTGAAAAAGTAACAATTAGAGCAGGGGAGTCAAAAGATATTTCTTTTAAG 2310 0 2020 .......................................................................................... 2109 I T P E M L K F Y N S D I Q F V N E V G E F E V M I G T N S Query_1 2311 ATCACTCCGGAAATGCTTAAGTTCTACAATTCGGATATCCAGTTTGTGAATGAAGTTGGTGAATTCGAAGTAATGATCGGAACGAACAGC 2400 0 2110 .......................................................................................... 2199 R D V K K A T F S L Query_1 2401 AGGGATGTGAAAAAAGCAACGTTTAGCTTG 2430 0 2200 .............................. 2229 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11985860 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,631 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115044.154 (APC115044.33-744.P(748).E487A.pMCSG68)
VESLLSKMTLEEKIGQMNQISSYGNIEDMSALIKKGEIGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQVAKDGARIAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKNVLRDEWGFDGFVVTDWASASEMISHGFAAGSKEVAMKSVNAGVDMEMVSYTFVKELPELVKEGKVKESTIDEAVRNILRIKYRLGLFDTPYVDEQQTSVMYAPSHLEAAKQAAVESAILLKNDKEVLPLQPSVKTVAVVGPMANAPYEQLGTWIFDGEKARTQTPLNAIKEMVGDKVQVIYEPGLAYSREKNPASVAKAAAAAARADVILAFVGEESILSGEAHCLADLDLQGDQGALITALAKTGKPVVTIVMAGRPLTIGKEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKAPVTFPRMVGQIPVYYAHNNTGRPATRNEVLLNDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNIKLASDVLKKDDVLTVTFDLENTGKYEGTEVAQLYIQDKIGSVTRPVKELKRFTRVTLKPGEKKSVSFELPVSELAFWNIDMAKVVEPGDFGLWVATDSQSGEEVFF
GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGTGCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAATCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCGTTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATTGATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGCAGCTATGGTAGAAGGTTTTCAGGGAGATTCGCTGAATAGTCCTACTTCAATTGCAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAGCCGAAGGAGGACGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAGCGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAATGTACTCCGTGACGAGTGGGGATTCGATGGTTTTGTTGTGACGGACTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGTTCAAAAGAAGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTGAAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCTTATGTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTGAAGAATGATAAGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTAGGTACTTGGATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATTTATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTGAATGATATTGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTTGTCGTACACCACATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATTCGATCTGGAAAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGAAAAAAAGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCTTC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,542 total letters Query= APC115044.154 Length=2541 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3844 0.0 1_01D_APC115044.154Seq171D01.seq 1844 0.0 2_D1_APC115044.154Seq171D07.seq 1707 0.0 V E S L L S K M T L E E K I G Q M N Q I S S Y G N I E D M S Query_1 202 GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGT 291 0 1 .......................................................................................... 90 A L I K K G E I G S I L N E V D P V R I N A L Q R V A M E E Query_1 292 GCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAA 381 0 91 .......................................................................................... 180 S R L G I P L L I A R D V I H G F K T I F P I P L G Q A A S Query_1 382 TCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCG 471 0 181 .......................................................................................... 270 F N P Q V A K D G A R I A A I E A S S V G I R W T F A P M I Query_1 472 TTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATT 561 0 271 .......................................................................................... 360 D I A R D P R W G R I A E G C G E D T Y L T S V M G A A M V Query_1 562 GATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGCAGCTATGGTA 651 0 361 .......................................................................................... 450 E G F Q G D S L N S P T S I A A C P K H F V G Y G A A E G G Query_1 652 GAAGGTTTTCAGGGAGATTCGCTGAATAGTCCTACTTCAATTGCAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAGCCGAAGGAGGA 741 0 451 .......................................................................................... 540 R D Y N S T F I P E R R L R N V Y L P P F E A A T K A G A Query_1 742 CGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAGCGGG-TGCAGC 830 0 541 ...................................................................................-...... 629 2 1151 ...A....NC.A..N...G.....GN..GAN 1121 A T F M T S F N D N D G I P S T G N A F I L K N V L R D Query_1 831 CACGTTTATGACTTCATTTAATGATAATG--ATGGAAT-CCCTTCTA-CCGGGAA-TGCTTTTATTTTGA-AGAATGTACT-CCGTGACG 913 0 630 .............................--.......-........-.......-..............-..........-........ 712 2 1120 ....N..NG.CT.....NNNN-.......GA.......A...G...NN.......A..N..NN.G....GG..G.......A........ 1032 E W G F D G F V V T D W A S A S E M I S H G F A A G Query_1 914 AGTGGGGA----TTCG-ATGGTTTTGTTGTGACGGACT-GGGCTTCTG-CCAGCGAAA-TGATAAGCC--ATGGTTTTGCCGCCGGTTC- 992 0 713 ........----....-.....................-.........-.........-.........--...................- 791 2 1031 -.A..N..GGNN....N....A....N.NNN...AN..A.......G.G.........A.......G.CA.NN...NN......A.A..C 943 S K E V A M K S V N A G V D M E M V S Y T F V K E L P E L V Query_1 993 -AAAAGAAGTGGCAATGAAATCTGTGAATGCAGGAG-TAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTG 1080 0 792 -...................................-..................................................... 879 2 942 A...............................N...G..................................................... 853 K E G K V K E S T I D E A V R N I L R I K Y R L G L F D T P Query_1 1081 AAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCT 1170 0 880 .......................................................................................... 969 2 852 ....................................................-..................................... 764 Y V D E Q Q T S V M Y A P S H L E A A K Q A A V E S A I L L Query_1 1171 TATGTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTG 1260 0 970 .......................................................................................... 1059 1 14 ...NN.......T 26 2 763 .......................................................................................... 674 K N D K E V L P L Q P S V K T V A V V G P M A N A P Y E Q L Query_1 1261 AAGAATGATAAGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTA 1350 0 1060 .......................................................................................... 1149 1 27 G...-..................................................................................... 115 2 673 .......................................................................................... 584 G T W I F D G E K A R T Q T P L N A I K E M V G D K V Q V I Query_1 1351 GGTACTTGGATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATT 1440 0 1150 .......................................................................................... 1239 1 116 .......................................................................................... 205 2 583 .......................................................................................... 494 Y E P G L A Y S R E K N P A S V A K A A A A A A R A D V I L Query_1 1441 TATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTT 1530 0 1240 .......................................................................................... 1329 0 1318 ............ 1307 1 206 .......................................................................................... 295 1 284 ............ 273 2 493 .......................................................................................... 404 2 415 ............ 426 A F V G E E S I L S G A A H C L A D L D L Q G D Q G A L I T Query_1 1531 GCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGCTGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACA 1620 0 1330 ..................................AA...................................................... 1419 1 296 .......................................................................................... 385 2 403 .......................................................................................... 314 A L A K T G K P V V T I V M A G R P L T I G K E V E E S T A Query_1 1621 GCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCT 1710 0 1420 .......................................................................................... 1509 1 386 .......................................................................................... 475 2 313 .......................................................................................... 224 V L Y S F H P G T M G G P A L A D L L W G K A V P S G K A P Query_1 1711 GTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCG 1800 0 1510 .......................................................................................... 1599 1 476 .......................................................................................... 565 2 223 .......................................................................................... 134 V T F P R M V G Q I P V Y Y A H N N T G R P A T R N E V L L Query_1 1801 GTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTG 1890 0 1600 .......................................................................................... 1689 0 1666 ............ 1655 1 566 .......................................................................................... 655 1 632 ............ 621 2 133 .......................................................................................... 44 2 67 ............ 78 N D I A V E A G Q T S L G C T S F Y M D A G F D P L F P F Query_1 1891 AATGATATTGCTGTTGAGGCAGG-ACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGG 1979 0 1690 .......................-.................................................................. 1778 1 656 .......................-.................................................................. 744 2 43 .......................A......... 11 G Y G L S Y T T F K Y S N I K L A S D V L K K D D V L T V T Query_1 1980 TT-ATGGCTTGTCGTACACCACATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACAT 2068 0 1779 ..-....................................................................................... 1867 1 745 ..A......................................................................................- 833 F D L E N T G K Y E G T E V A Q L Y I Q D K I G S V T R P V Query_1 2069 TCGATCTGGAAAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGA 2158 0 1868 .......................................................................................... 1957 0 1926 ............ 1915 1 834 ...................................................TN..................................... 923 K E L K R F T R V T L K P G E K K S V S F E L P V S E L A F Query_1 2159 AAGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGaaaaaaaGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTT 2248 0 1958 .......................................................................................... 2047 1 924 ..................................N.......GAG..................-.......................... 1012 W N I D M A K V V E P G D F G L W V A T D S Q S G E E V F Query_1 2249 GGAACATAGATATGGCTAAAGTTGTGG-AACCCGGAGACTTTGGGCTTTGGG-TGGCAACGGAT-AGTCAGTCCGGAGAAGAAGTTTTC- 2334 0 2048 ...........................-........................-...........-........................- 2133 1 1013 ...........................A...T.........C..........T...........N....--NT.NN.A...-.......T 1099 F * H W K W I T D P N S S S V D K L A A A L E H H H H H H Query_1 2335 TTCTAACATT-GGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTG 2423 0 2134 ... 2136 1 1100 ..........G.........N.NG.AT..... 1131 * D P A A N K A R Query_1 2424 AGATCCGGCTGCTAACAAAGCCCGAA 2449 1 1062 ........... 1052 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11335284 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,542 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115044.155 (APC115044.33-744.P(748).W418A.pMCSG68)
VESLLSKMTLEEKIGQMNQISSYGNIEDMSALIKKGEIGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQVAKDGARIAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKNVLRDEWGFDGFVVTDWASASEMISHGFAAGSKEVAMKSVNAGVDMEMVSYTFVKELPELVKEGKVKESTIDEAVRNILRIKYRLGLFDTPYVDEQQTSVMYAPSHLEAAKQAAVESAILLKNDKEVLPLQPSVKTVAVVGPMANAPYEQLGTWIFDGEKARTQTPLNAIKEMVGDKVQVIYEPGLAYSREKNPASVAKAAAAAARADVILAFVGEESILSGEAHCLADLDLQGDQGALITALAKTGKPVVTIVMAGRPLTIGKEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKAPVTFPRMVGQIPVYYAHNNTGRPATRNEVLLNDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNIKLASDVLKKDDVLTVTFDLENTGKYEGTEVAQLYIQDKIGSVTRPVKELKRFTRVTLKPGEKKSVSFELPVSELAFWNIDMAKVVEPGDFGLWVATDSQSGEEVFF
GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGTGCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAATCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCGTTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATTGATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGCAGCTATGGTAGAAGGTTTTCAGGGAGATTCGCTGAATAGTCCTACTTCAATTGCAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAGCCGAAGGAGGACGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAGCGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAATGTACTCCGTGACGAGTGGGGATTCGATGGTTTTGTTGTGACGGACTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGTTCAAAAGAAGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTGAAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCTTATGTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTGAAGAATGATAAGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTAGGTACTTGGATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATTTATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTGAATGATATTGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTTGTCGTACACCACATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATTCGATCTGGAAAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGAAAAAAAGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCTTC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,541 total letters Query= APC115044.155 Length=2541 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3838 0.0 2_E1_APC115044.155Seq171E07.seq 1898 0.0 1_01E_APC115044.155Seq171E01.seq 1820 0.0 V E S L L S K M T L E E K I G Q M N Q I S S Y G N I E D M Query_1 202 GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGT-CAG-ATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGA 289 0 1 .............................................-...-........................................ 88 2 1227 ........N....A...G....N.-.NN.N..-...AC....N.N........NNNNG 1172 S A L I K K G E I G S I L N E V D P V R I N A L Q R V A M Query_1 290 GTGCTTTGATTAAGAAA-GGTGAAATCGGTTCCAT-CTTGAATGAGGTGGATCCGGTGCGTATTAATGCG-CTACAGCGCGTGGCAATGG 376 0 89 .................-.................-..................................-................... 175 2 1171 .....GA-......G..CN.N...T...A......N.....-...A....N..........NA.......G........N......N... 1084 E E S R L G I P L L I A R D V I H G F K T I F P I P L G Q A Query_1 377 AAGAATCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAG-CG 465 0 176 .......................................................................................-.. 264 2 1083 ...G......ANG.............A............NN-.......AC..G..G...........N.........G..A.....G.. 995 A S F N P Q V A K D G A R I A A I E A S S V G I R W T F A Query_1 466 GCTTCGTTCAATCC-GCAGGTAGCGAAAGACGGTGCACGGA-TAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGC 553 0 265 ..............-..........................-................................................ 352 2 994 ..............N.......N........N.N.......A..............................................A. 905 P M I D I A R D P R W G R I A E G C G E D T Y L T S V M G Query_1 554 C-AATGATTGATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAA-GGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGC 641 0 353 .-..............................................-......................................... 440 2 904 .C..............................................A......................................... 815 A A M V E G F Q G D S L N S P T S I A A C P K H F V G Y G A Query_1 642 AGCTATGGTAGAAGGTTTTCAGGGAGATTCGCTGAA-TAGTCCTACTTCAATTGCAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAG 730 0 441 ....................................-..................................................... 529 2 814 ....................................A..................................................... 725 A E G G R D Y N S T F I P E R R L R N V Y L P P F E A A T K Query_1 731 CCGAAGGAGGACGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAG 820 0 530 .......................................................................................... 619 2 724 .......................................................................................... 635 A G A A T F M T S F N D N D G I P S T G N A F I L K N V L R Query_1 821 CGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAATGTACTCCGTG 910 0 620 .......................................................................................... 709 2 634 .......................................................................................... 545 D E W G F D G F V V T D W A S A S E M I S H G F A A G S K E Query_1 911 ACGAGTGGGGATTCGATGGTTTTGTTGTGACGGACTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGTTCAAAAGAAG 1000 0 710 .......................................................................................... 799 2 544 .......................................................................................... 455 V A M K S V N A G V D M E M V S Y T F V K E L P E L V K E G Query_1 1001 TGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTGAAAGAGGGAA 1090 0 800 .......................................................................................... 889 2 454 .......................................................................................... 365 K V K E S T I D E A V R N I L R I K Y R L G L F D T P Y V D Query_1 1091 AGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCTTATGTAGATG 1180 0 890 .......................................................................................... 979 2 364 .......................................................................................... 275 E Q Q T S V M Y A P S H L E A A K Q A A V E S A I L L K N D Query_1 1181 AACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTGAAGAATGATA 1270 0 980 .......................................................................................... 1069 2 274 .......................................................................................... 185 1 9 .........G--...... 24 K E V L P L Q P S V K T V A V V G P M A N A P Y E Q L G T A Query_1 1271 AGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTAGGTACTGCTA 1360 0 1070 ......................................................................................TGG. 1159 2 184 .......................................................................................... 95 1 25 ............................................N............................................. 114 I F D G E K A R T Q T P L N A I K E M V G D K V Q V I Y E P Query_1 1361 TATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATTTATGAACCGG 1450 0 1160 .......................................................................................... 1249 2 94 ..................................................................................NA....C. 5 1 115 .......................................................................................... 204 G L A Y S R E K N P A S V A K A A A A A A R A D V I L A F V Query_1 1451 GACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGG 1540 0 1250 .......................................................................................... 1339 0 1318 ............ 1307 2 4 .... 1 1 205 .......................................................................................... 294 1 273 ............ 262 G E E S I L S G E A H C L A D L D L Q G D Q G A L I T A L A Query_1 1541 GTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGCTA 1630 0 1340 .......................................................................................... 1429 1 295 .......................................................................................... 384 K T G K P V V T I V M A G R P L T I G K E V E E S T A V L Y Query_1 1631 AGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTATT 1720 0 1430 .......................................................................................... 1519 1 385 .......................................................................................... 474 S F H P G T M G G P A L A D L L W G K A V P S G K A P V T F Query_1 1721 CATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTTCC 1810 0 1520 .......................................................................................... 1609 1 475 .......................................................................................... 564 P R M V G Q I P V Y Y A H N N T G R P A T R N E V L L N D I Query_1 1811 CGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTGAATGATATTG 1900 0 1610 .......................................................................................... 1699 0 1666 ............ 1655 1 565 .......................................................................................... 654 1 621 ............ 610 A V E A G Q T S L G C T S F Y M D A G F D P L F P F G Y G L Query_1 1901 CTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTTGT 1990 0 1700 .......................................................................................... 1789 1 655 .......................................................................................... 744 S Y T T F K Y S N I K L A S D V L K K D D V L T V T F D L E Query_1 1991 CGTACACCACATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATTCGATCTGGAAA 2080 0 1790 .......................................................................................... 1879 1 745 .......................................................................................... 834 N T G K Y E G T E V A Q L Y I Q D K I G S V T R P V K E L K Query_1 2081 ATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAAAC 2170 0 1880 .......................................................................................... 1969 0 1926 ............ 1915 1 835 ..................................T.......................N..................-............ 923 1 881 ............ 870 R F T R V T L K P G E K K S V S F E L P V S E L A F W N I D Query_1 2171 GCTTCACTCGTGTGACATTGAAGCCGGGTGAGaaaaaaaGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTTGGAACATAGATA 2260 0 1970 .......................................................................................... 2059 1 924 .........N.............N.C.........................-......................-............... 1011 M A K V V E P G D F G L W V A T D S Q S G E E V F F * H W K Query_1 2261 TGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCTTCTAACATTGGAAGT 2350 0 2060 ............................................................................. 2136 1 1012 .-...-.......-...............-..N......N.--CN.C...........T.----...GAG........-....--.GN.. 1088 W I T D P N S S S V D K L A A A Query_1 2351 GGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCAC 2398 1 1089 ....-.......--G....N.....GT.....-.......-....... 1131 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11332760 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,541 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115044.156 (APC115044.33-744.P(748).D104A.pMCSG68)
VESLLSKMTLEEKIGQMNQISSYGNIEDMSALIKKGEIGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQVAKDGARIAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKNVLRDEWGFDGFVVTDWASASEMISHGFAAGSKEVAMKSVNAGVDMEMVSYTFVKELPELVKEGKVKESTIDEAVRNILRIKYRLGLFDTPYVDEQQTSVMYAPSHLEAAKQAAVESAILLKNDKEVLPLQPSVKTVAVVGPMANAPYEQLGTWIFDGEKARTQTPLNAIKEMVGDKVQVIYEPGLAYSREKNPASVAKAAAAAARADVILAFVGEESILSGEAHCLADLDLQGDQGALITALAKTGKPVVTIVMAGRPLTIGKEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKAPVTFPRMVGQIPVYYAHNNTGRPATRNEVLLNDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNIKLASDVLKKDDVLTVTFDLENTGKYEGTEVAQLYIQDKIGSVTRPVKELKRFTRVTLKPGEKKSVSFELPVSELAFWNIDMAKVVEPGDFGLWVATDSQSGEEVFF
GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGTGCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAATCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCGTTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATTGATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGCAGCTATGGTAGAAGGTTTTCAGGGAGATTCGCTGAATAGTCCTACTTCAATTGCAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAGCCGAAGGAGGACGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAGCGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAATGTACTCCGTGACGAGTGGGGATTCGATGGTTTTGTTGTGACGGACTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGTTCAAAAGAAGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTGAAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCTTATGTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTGAAGAATGATAAGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTAGGTACTTGGATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATTTATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTGAATGATATTGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTTGTCGTACACCACATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATTCGATCTGGAAAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGAAAAAAAGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCTTC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,554 total letters Query= APC115044.156 Length=2541 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3847 0.0 1_01F_F1_APC115044.156_T7S.seq 2028 0.0 2_F1_APC115044.156Seq171F07.seq 1876 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 57 ATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTG 146 1 11 .......................................................................................... 100 W S H P Q F E K G T E N L Y F Q S N A V E S L L S K M T L E Query_1 147 GTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGA 236 0 1 ................................... 35 1 101 .......................................................................................... 190 E K I G Q M N Q I S S Y G N I E D M S A L I K K G E I G S I Query_1 237 GAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGTGCTTTGATTAAGAAAGGTGAAATCGGTTCCA-TCT 325 0 36 ......................................................................................-... 124 1 191 ......................................................................................-... 279 2 1154 ......-..C......G..N-..N.........A... 1120 L N E V D P V R I N A L Q R V A M E E S R L G I P L L I A Query_1 326 TGAATGAGGTGGATCCGGTGCGTATTAAT-GCGCTACAGCG-CGTGGCAATGGAAGAATCCCGTTTGGGTATTCCTTTATTGATAGCGCG 413 0 125 .............................-...........-................................................ 212 1 280 .............................-...........-................................................ 367 2 1119 G..N.NG.......T..NNN-........N..........NN....C............N...G......NN........G........N 1031 R A V I H G F K T I F P I P L G Q A A S F N P Q V A K D G A Query_1 414 TGCTGTCATTCACGGG-TTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCGTTCAATCCGCAGGTAGCGAAAGACGGTGCAC 502 0 213 ..A.............-......................................................................... 301 1 368 ................-......................................................................... 456 2 1030 ........N....N..A................NN.......-...............N.N-...T.................C...... 943 R I A A I E A S S V G I R W T F A P M I D I A R D P R W G R Query_1 503 GGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATTGATATTGCCCGCGATCCTCGCTGGGGACGTA 592 0 302 .......................................................................................... 391 1 457 .................................................A........................................ 546 2 942 ........N........................................A..................-..................... 854 I A E G C G E D T Y L T S V M G A A M V E G F Q G D S L N S Query_1 593 TTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGCAGCTATGGTAGAAGGTTTTCAGGGAGATTCGCTGAATAGTC 682 0 392 .......................................................................................... 481 1 547 .......................................................................................... 636 2 853 .......................................................................................... 764 P T S I A A C P K H F V G Y G A A E G G R D Y N S T F I P E Query_1 683 CTACTTCAATTGCAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAGCCGAAGGAGGACGTGATTATAATTCCACGTTCATTCCCGAAC 772 0 482 .......................................................................................... 571 1 637 .......................................................................................... 726 2 763 .......................................................................................... 674 R R L R N V Y L P P F E A A T K A G A A T F M T S F N D N D Query_1 773 GTCGTCTGCGCAATG-TTTATTTGCCACCTTTTGAAGCTGCCACCAAAGCGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGAT 861 0 572 ...............-.......................................................................... 660 1 727 ...............T.......................................................................... 816 2 673 ...............-.......................................................................... 585 G I P S T G N A F I L K N V L R D E W G F D G F V V T D W A Query_1 862 GGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAATGTACTCCGTGACGAGTGGGGATTCGATGGTTTTGTTGTGACGGACTGGGCT 951 0 661 .......................................................................................... 750 1 817 .......................................................................................... 906 2 584 .......................................................................................... 495 S A S E M I S H G F A A G S K E V A M K S V N A G V D M E M Query_1 952 TCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGTTCAAAAGAAGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATG 1041 0 751 .......................................................................................... 840 1 907 .........................................-...............-................................ 994 2 494 .......................................................................................... 405 V S Y T F V K E L P E L V K E G K V K E S T I D E A V R N Query_1 1042 GTGAGTTACACTTTTGTGAAGGAA-CTGCCGGAATTAGTGAAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATAT 1130 0 841 ........................-................................................................. 929 1 995 ........................C....................-.......................................-.N.. 1082 2 404 ........................-................................................................. 316 I L R I K Y R L G L F D T P Y V D E Q Q T S V M Y A P S H L Query_1 1131 TTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCTTATGTAGATGAACAACAAACATCTGTCATGTATG-CTCCTTCTCATTTGG 1219 0 930 ..........................................................................-............... 1018 1 1083 ........................GA..........................AC.NC.............NN..C....N..N.....NN 1172 2 315 ..........................................................................-............... 227 E A A K Q A A V E S A I L L K N D K E V L P L Q P S V K T V Query_1 1220 AAGCAGCTAAGCAAGCCGCTG-TTGAATCGGCTATTCTGTTGAAGAATGATAAGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTT 1308 0 1019 .....................-.................................................................... 1107 1 1173 ....-NN.T...T........T.........N.......N... 1214 2 226 .....................-.................................................................... 138 A V V G P M A N A P Y E Q L G T W I F D G E K A R T Q T P L Query_1 1309 GCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTAGGTACTTGGATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTG 1398 0 1108 .......................................................................................... 1197 2 137 .......................................................................................... 48 N A I K E M V G D K V Q V I Y E P G L A Y S R E K N P A S V Query_1 1399 AACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATTTATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTG 1488 0 1198 .......................................................................................... 1287 2 47 .....................................-.... 7 A K A A A A A A R A D V I L A F V G E E S I L S G E A H C L Query_1 1489 GCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTG 1578 0 1288 .......................................................................................... 1377 0 1318 ............ 1307 A D L D L Q G D Q G A L I T A L A K T G K P V V T I V M A G Query_1 1579 GCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGT 1668 0 1378 .......................................................................................... 1467 R P L T I G K E V E E S T A V L Y S F H P G T M G G P A L A Query_1 1669 CGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCT 1758 0 1468 .......................................................................................... 1557 D L L W G K A V P S G K A P V T F P R M V G Q I P V Y Y A H Query_1 1759 GATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCAT 1848 0 1558 .......................................................................................... 1647 N N T G R P A T R N E V L L N D I A V E A G Q T S L G C T S Query_1 1849 AATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTGAATGATATTGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCC 1938 0 1648 .......................................................................................... 1737 0 1666 ............ 1655 F Y M D A G F D P L F P F G Y G L S Y T T F K Y S N I K L A Query_1 1939 TTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTTGTCGTACACCACATTTAAGTATAGCAACATCAAACTGGCG 2028 0 1738 .......................................................................................... 1827 S D V L K K D D V L T V T F D L E N T G K Y E G T E V A Q L Query_1 2029 TCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATTCGATCTGGAAAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTG 2118 0 1828 .......................................................................................... 1917 0 1926 ... 1924 Y I Q D K I G S V T R P V K E L K R F T R V T L K P G E K K Query_1 2119 TATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGaaaaaa 2208 0 1918 .......................................................................................... 2007 0 1923 ......... 1915 S V S F E L P V S E L A F W N I D M A K V V E P G D F G L W Query_1 2209 aGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGG 2298 0 2008 .......................................................................................... 2097 V A T D S Q S G E E V F F Query_1 2299 GTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCTTC 2337 0 2098 ....................................... 2136 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11365572 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,554 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115044.157 (APC115044.33-744.P(748).R586A.pMCSG68)
VESLLSKMTLEEKIGQMNQISSYGNIEDMSALIKKGEIGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQVAKDGARIAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKNVLRDEWGFDGFVVTDWASASEMISHGFAAGSKEVAMKSVNAGVDMEMVSYTFVKELPELVKEGKVKESTIDEAVRNILRIKYRLGLFDTPYVDEQQTSVMYAPSHLEAAKQAAVESAILLKNDKEVLPLQPSVKTVAVVGPMANAPYEQLGTWIFDGEKARTQTPLNAIKEMVGDKVQVIYEPGLAYSREKNPASVAKAAAAAARADVILAFVGEESILSGEAHCLADLDLQGDQGALITALAKTGKPVVTIVMAGRPLTIGKEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKAPVTFPRMVGQIPVYYAHNNTGRPATRNEVLLNDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNIKLASDVLKKDDVLTVTFDLENTGKYEGTEVAQLYIQDKIGSVTRPVKELKRFTRVTLKPGEKKSVSFELPVSELAFWNIDMAKVVEPGDFGLWVATDSQSGEEVFF
GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGTGCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAATCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCGTTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATTGATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGCAGCTATGGTAGAAGGTTTTCAGGGAGATTCGCTGAATAGTCCTACTTCAATTGCAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAGCCGAAGGAGGACGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAGCGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAATGTACTCCGTGACGAGTGGGGATTCGATGGTTTTGTTGTGACGGACTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGTTCAAAAGAAGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTGAAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCTTATGTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTGAAGAATGATAAGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTAGGTACTTGGATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATTTATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTGAATGATATTGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTTGTCGTACACCACATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATTCGATCTGGAAAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGAAAAAAAGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCTTC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,526 total letters Query= APC115044.157 Length=2541 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3844 0.0 1_01G_APC115044.157Seq171G01.seq 1817 0.0 2_G1_APC115044.157Seq171G07.seq 1804 0.0 V E S L L S K M T L E E K I G Q M N Q I S S Y G N I E D M S Query_1 202 GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGT 291 0 1 .......................................................................................... 90 A L I K K G E I G S I L N E V D P V R I N A L Q R V A M E E Query_1 292 GCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAA 381 0 91 .......................................................................................... 180 S R L G I P L L I A R D V I H G F K T I F P I P L G Q A A S Query_1 382 TCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCG 471 0 181 .......................................................................................... 270 F N P Q V A K D G A R I A A I E A S S V G I R W T F A P M I Query_1 472 TTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATT 561 0 271 .......................................................................................... 360 D I A R D P R W G R I A E G C G E D T Y L T S V M G A A M V Query_1 562 GATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGCAGCTATGGTA 651 0 361 .......................................................................................... 450 E G F Q G D S L N S P T S I A A C P K H F V G Y G A A E G G Query_1 652 GAAGGTTTTCAGGGAGATTCGCTGAATAGTCCTACTTCAATTGCAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAGCCGAAGGAGGA 741 0 451 .......................................................................................... 540 R D Y N S T F I P E R R L R N V Y L P P F E A A T K A G Query_1 742 CGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACC---TTTTGAAGCT-GCCAC-CAAAG-CGGGT 825 0 541 ...........................................................---..........-.....-.....-..... 624 2 1165 ......T....CTA...G.....NN...N.A.....N..A.N 1124 A A T F M T S F N D N D G I P S T G N A F I L K N V L Query_1 826 GCAGCCACG-TTTATG-ACTTCATTTAATG-ATAATGATGGAATCCC-TTCTA-CCGGGAAT-GCTTTTATTTTGAA-GAATGTA-CT-C 906 0 625 .........-......-.............-................-.....-........-..............-.......-..-. 705 2 1123 .....AT..N......G......N..T...G....A...........C....NN........A.N..N.....N...A.......A..A. 1034 R D E W G F D G F V V T D W A S A S E M I S H G F A A G S Query_1 907 CGTGACGAG-TGGGGATTCGA-TGGTTTTGTTGTGACGGACTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGTTCAA 994 0 706 .........-...........-.................................................................... 793 2 1033 .........G...N.......A.........N....N..........................................N....-..... 945 K E V A M K S V N A G V D M E M V S Y T F V K E L P E L V Query_1 995 AAGAAGTGGCAATG-AAATCTGTGAATGCAGGAGTAGATATGGAAATGG-TGAGTTACA-CTTTTGTGAAGGAACTGCCGGAA-TTAGTG 1080 0 794 ..............-..................................-.........-.......................-...... 879 2 944 .GA...........N..........................N.N.....N.........A.......................A.....N 855 K E G K V K E S T I D E A V R N I L R I K Y R L G L F D T P Query_1 1081 AAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCT 1170 0 880 .......................................................................................... 969 2 854 ...........A...............................N........................................N.C... 765 Y V D E Q Q T S V M Y A P S H L E A A K Q A A V E S A I L L Query_1 1171 TATGTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTG 1260 0 970 .......................................................................................... 1059 1 8 ....-........ 19 2 764 .......................................................................................... 675 K N D K E V L P L Q P S V K T V A V V G P M A N A P Y E Q L Query_1 1261 AAGAATGATAAGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTA 1350 0 1060 .......................................................................................... 1149 1 20 .G--...................................................................................... 107 2 674 .......................................................................................... 585 G T W I F D G E K A R T Q T P L N A I K E M V G D K V Q V I Query_1 1351 GGTACTTGGATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATT 1440 0 1150 .......................................................................................... 1239 1 108 .......................................................................................... 197 2 584 .......................................................................................... 495 Y E P G L A Y S R E K N P A S V A K A A A A A A R A D V I L Query_1 1441 TATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTT 1530 0 1240 .......................................................................................... 1329 0 1318 ............ 1307 1 198 .......................................................................................... 287 1 276 ............ 265 2 494 .......................................................................................... 405 2 416 ............ 427 A F V G E E S I L S G E A H C L A D L D L Q G D Q G A L I T Query_1 1531 GCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACA 1620 0 1330 .......................................................................................... 1419 1 288 .......................................................................................... 377 2 404 .......................................................................................... 315 A L A K T G K P V V T I V M A G R P L T I G K E V E E S T A Query_1 1621 GCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCT 1710 0 1420 .......................................................................................... 1509 1 378 .......................................................................................... 467 2 314 .......................................................................................... 225 V L Y S F H P G T M G G P A L A D L L W G K A V P S G K A P Query_1 1711 GTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCG 1800 0 1510 .......................................................................................... 1599 1 468 .......................................................................................... 557 2 224 .......................................................................................... 135 V T F P R M V G Q I P V Y Y A H N N T G A P A T R N E V L L Query_1 1801 GTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGAGCTCCGGCTACACGGAATGAAGTGTTGCTG 1890 0 1600 ............................................................CG............................ 1689 1 558 .......................................................................................... 647 1 624 ............ 613 2 134 .......................................................................................... 45 2 68 ............ 79 N D I A V E A G Q T S L G C T S F Y M D A G F D P L F P F Query_1 1891 AATGATATTGCTGTTGAGGCAGGACAGACTTCA-CTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGG 1979 0 1690 .................................-........................................................ 1778 1 648 .................................-........................................................ 736 2 44 .................................N..N... 5 G Y G L S Y T T F K Y S N I K L A S D V L K K D D V L T V T Query_1 1980 TTATGGCTTGTCGTACACCACA-TTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACAT 2068 0 1779 ......................-................................................................... 1867 1 737 ......................T................................................................... 826 F D L E N T G K Y E G T E V A Q L Y I Q D K I G S V T R P Query_1 2069 TCGATCTGGAAAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGT-GAC-TCGTCCGGT 2156 0 1868 ............................................................................-...-......... 1955 0 1926 ............ 1915 1 827 ............................................................................N...T....-.... 915 1 885 ............ 874 V K E L K R F T R V T L K P G E K K S V S F E L P V S E L A Query_1 2157 GAAAGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGG-TGAGaaaaaaaGCGTTTCGTTTGAACT-CCCTGTTAGTGAACTTGCA 2244 0 1956 ..........................................-...........................-................... 2043 1 916 ............N.............................T...........................N...N............... 1005 F W N I D M A K V V E P G D F G L W V A T D S Q S G E E Query_1 2245 TTTTGGAACATAGATATGGCTAAAGTTGTGGAA--CCCGGAGACTTTGGGCTTTGGGTGGCAACGGAT-AGTCAGTCCGGAG-AAGAAGT 2330 0 2044 .................................--.................................-.............-....... 2129 1 1006 ..C......N.......................AC.N.........--.N....N..NN..C......A.......TNN..NT....T.. 1093 V F F * H W K W I Query_1 2331 TTT--CTTCTAACATTGGAAGTGGATAAC 2357 0 2130 ...--.... 2136 1 1094 ...TCT.....NNT......NN....... 1122 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11294900 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,526 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115044.158 (APC115044.33-744.P(748).W418F.pMCSG68)
VESLLSKMTLEEKIGQMNQISSYGNIEDMSALIKKGEIGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQVAKDGARIAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKNVLRDEWGFDGFVVTDWASASEMISHGFAAGSKEVAMKSVNAGVDMEMVSYTFVKELPELVKEGKVKESTIDEAVRNILRIKYRLGLFDTPYVDEQQTSVMYAPSHLEAAKQAAVESAILLKNDKEVLPLQPSVKTVAVVGPMANAPYEQLGTWIFDGEKARTQTPLNAIKEMVGDKVQVIYEPGLAYSREKNPASVAKAAAAAARADVILAFVGEESILSGEAHCLADLDLQGDQGALITALAKTGKPVVTIVMAGRPLTIGKEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKAPVTFPRMVGQIPVYYAHNNTGRPATRNEVLLNDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNIKLASDVLKKDDVLTVTFDLENTGKYEGTEVAQLYIQDKIGSVTRPVKELKRFTRVTLKPGEKKSVSFELPVSELAFWNIDMAKVVEPGDFGLWVATDSQSGEEVFF
GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGTGCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAATCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCGTTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATTGATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGCAGCTATGGTAGAAGGTTTTCAGGGAGATTCGCTGAATAGTCCTACTTCAATTGCAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAGCCGAAGGAGGACGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAGCGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAATGTACTCCGTGACGAGTGGGGATTCGATGGTTTTGTTGTGACGGACTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGTTCAAAAGAAGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTGAAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCTTATGTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTGAAGAATGATAAGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTAGGTACTTGGATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATTTATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTGAATGATATTGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTTGTCGTACACCACATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATTCGATCTGGAAAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGAAAAAAAGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCTTC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,628 total letters Query= APC115044.158 Length=2541 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3844 0.0 1_01H_APC115044.158Seq171H01.seq 1703 0.0 2_H1_APC115044.158Seq171H07.seq 1077 0.0 V E S L L S K M T L E E K I G Q M N Q I S S Y G N I E D M S Query_1 202 GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGT 291 0 1 .......................................................................................... 90 A L I K K G E I G S I L N E V D P V R I N A L Q R V A M E E Query_1 292 GCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAA 381 0 91 .......................................................................................... 180 S R L G I P L L I A R D V I H G F K T I F P I P L G Q A A Query_1 382 TCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAA--TTTTCCCTATTCCCTTGGGACAAGCGGCTT 469 0 181 ..........................................................--.............................. 268 2 1104 ...........NC.TT........T.. 1078 S F N P Q V A K D G A R I A A I E A S S V G I R W T F A P M Query_1 470 CGTTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGA 559 0 269 .......................................................................................... 358 I D I A R D P R W G R I A E G C G E D T Y L T S V M G A Query_1 560 TTGATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATAC-GTACCTT-ACTT--CCGTAATGGG-AGCAG- 643 0 359 ..........................................................-.......-....--..........-.....- 442 2 894 ........GA.......C..T....TN...TC..........G...NNA 846 A M V E G F Q G D S L N S P T S I A A C P K H F V G Y Query_1 644 CTAT-GGTA-GAAGGTTTT-CAGGGA-GATT-CGCTGAA-TAGTCCTAC-TTCAATTGCAGCTTGCCCTAAA-CATTTT-GTAGG-TTAC 723 0 443 ....-....-.........-......-....-.......-.........-......................-......-.....-.... 522 2 845 ....N....A.........T......A....T.......A.........C......N...............N......T.....G.... 756 G A A E G G R D Y N S T F I P E R R L R N V Y L P P F E A A Query_1 724 GGTGCAGCCGAAGGAGGACGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCC 813 0 523 .......................................................................................... 612 2 755 N......................................................................................N.. 666 T K A G A A T F M T S F N D N D G I P S T G N A F I L K N V Query_1 814 ACCAAAGCGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAATGTA 903 0 613 .......................................................................................... 702 2 665 ..........N.....................................................A...............N.N...N... 576 L R D E W G F D G F V V T D W A S A S E M I S H G F A A G S Query_1 904 CTCCGTGACGAGTGGGGATTCGATGGTTTTGTTGTGACGGACTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGTTCA 993 0 703 .......................................................................................... 792 2 575 ...............A...................................................N..........N..N........ 486 K E V A M K S V N A G V D M E M V S Y T F V K E L P E L V K Query_1 994 AAAGAAGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTGAAA 1083 0 793 .......................................................................................... 882 2 485 G...................................N..N........N........................NN.....N......... 396 E G K V K E S T I D E A V R N I L R I K Y R L G L F D T P Y Query_1 1084 GAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCTTAT 1173 0 883 .......................................................................................... 972 2 395 .................T.....NNN.............................................N.................. 306 V D E Q Q T S V M Y A P S H L E A A K Q A A V E S A I L L K Query_1 1174 GTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTGAAG 1263 0 973 .......................................................................................... 1062 1 5 ...CN.........G- 19 2 305 .........................N.........................................N................N..... 216 N D K E V L P L Q P S V K T V A V V G P M A N A P Y E Q L Query_1 1264 AATGATAAGGAAGTGTTGCCGTTACAGCCATCTGTGAAAA-CTGTTGCAGTGGTAGGA-CCTATGGCTAATGCACCTTATGAACAGTTAG 1351 0 1063 ........................................-.................-............................... 1150 1 20 -.......................................-............A....-............................... 106 2 215 .NN.....N.......................N.......G...........A.....G...........N..G...N....N.. 131 G T F I F D G E K A R T Q T P L N A I K E M V G D K V Q V I Query_1 1352 GTACTTTTATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATTT 1441 0 1151 ......GG.................................................................................. 1240 1 107 .......................................................................................... 196 Y E P G L A Y S R E K N P A S V A K A A A A A A R A D V I L Query_1 1442 ATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTTG 1531 0 1241 .......................................................................................... 1330 0 1318 ............ 1307 1 197 .......................................................................................... 286 1 274 ............ 263 A F V G E E S I L S G E A H C L A D L D L Q G D Q G A L I T Query_1 1532 CTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACAG 1621 0 1331 .......................................................................................... 1420 1 287 .......................................................................................... 376 A L A K T G K P V V T I V M A G R P L T I G K E V E E S T A Query_1 1622 CTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCTG 1711 0 1421 .......................................................................................... 1510 1 377 .......................................................................................... 466 V L Y S F H P G T M G G P A L A D L L W G K A V P S G K A P Query_1 1712 TTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCGG 1801 0 1511 .......................................................................................... 1600 1 467 .......................................................................................... 556 V T F P R M V G Q I P V Y Y A H N N T G R P A T R N E V L L Query_1 1802 TCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTGA 1891 0 1601 .......................................................................................... 1690 0 1666 ............ 1655 1 557 .......................................................................................... 646 1 622 ............ 611 N D I A V E A G Q T S L G C T S F Y M D A G F D P L F P F G Query_1 1892 ATGATATTGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCC-TTGTTTCCGTTTGGT 1980 0 1691 ..........................................................................-............... 1779 1 647 ..........................................................................T............... 736 Y G L S Y T T F K Y S N I K L A S D V L K K D D V L T V T Query_1 1981 TATGGCTTG-TCGTACACCACATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATT 2069 0 1780 .........-................................................................................ 1868 1 737 .........T..............................N....................-............................ 825 F D L E N T G K Y E G T E V A Q L Y I Q D K I G S V T R P V Query_1 2070 CGATCTGGAAAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAA 2159 0 1869 .......................................................................................... 1958 0 1926 ............ 1915 1 826 ...................-.......N.........-.N....-.......................................N..... 912 1 880 ............ 869 K E L K R F T R V T L K P G E K K S V S F E L P V S E L A F Query_1 2160 AGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGaaaaaaaGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTTG 2249 0 1959 .......................................................................................... 2048 1 913 ...............NN..............-.....T.....-.........-...T..G..N.......N........-...N...-- 996 W N I D M A K V V E P G D F G L W V A T D S Q S G E E V F F Query_1 2250 GAACATAGATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAG-TCAGTCCGGAGAAGAAGTTTTCTTCT 2338 0 2049 ...............................................................-......................... 2136 1 997 .G.NN..N..N..C......-...-.........N.AC...NCT..G.......C........T..N..NN.A.AN..-........... 1083 * H W K Query_1 2339 AACATTGGAAG 2349 1 1084 ...N....C.. 1094 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11552348 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,628 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115044.159 (APC115044.33-744.P(748).W281F.pMCSG68)
VESLLSKMTLEEKIGQMNQISSYGNIEDMSALIKKGEIGSILNEVDPVRINALQRVAMEESRLGIPLLIARDVIHGFKTIFPIPLGQAASFNPQVAKDGARIAAIEASSVGIRWTFAPMIDIARDPRWGRIAEGCGEDTYLTSVMGAAMVEGFQGDSLNSPTSIAACPKHFVGYGAAEGGRDYNSTFIPERRLRNVYLPPFEAATKAGAATFMTSFNDNDGIPSTGNAFILKNVLRDEWGFDGFVVTDWASASEMISHGFAAGSKEVAMKSVNAGVDMEMVSYTFVKELPELVKEGKVKESTIDEAVRNILRIKYRLGLFDTPYVDEQQTSVMYAPSHLEAAKQAAVESAILLKNDKEVLPLQPSVKTVAVVGPMANAPYEQLGTWIFDGEKARTQTPLNAIKEMVGDKVQVIYEPGLAYSREKNPASVAKAAAAAARADVILAFVGEESILSGEAHCLADLDLQGDQGALITALAKTGKPVVTIVMAGRPLTIGKEVEESTAVLYSFHPGTMGGPALADLLWGKAVPSGKAPVTFPRMVGQIPVYYAHNNTGRPATRNEVLLNDIAVEAGQTSLGCTSFYMDAGFDPLFPFGYGLSYTTFKYSNIKLASDVLKKDDVLTVTFDLENTGKYEGTEVAQLYIQDKIGSVTRPVKELKRFTRVTLKPGEKKSVSFELPVSELAFWNIDMAKVVEPGDFGLWVATDSQSGEEVFF
GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGTGCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAATCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCGTTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATTGATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGCAGCTATGGTAGAAGGTTTTCAGGGAGATTCGCTGAATAGTCCTACTTCAATTGCAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAGCCGAAGGAGGACGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAGCGGGTGCAGCCACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAATGTACTCCGTGACGAGTGGGGATTCGATGGTTTTGTTGTGACGGACTGGGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGTTCAAAAGAAGTGGCAATGAAATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTGAAAGAGGGAAAGGTGAAGGAAAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCTTATGTAGATGAACAACAAACATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTGAAGAATGATAAGGAAGTGTTGCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTAGGTACTTGGATATTTGATGGTGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATTTATGAACCGGGACTAGCATATAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAATCTATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGCTAAGACGGGTAAACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTATTCATTCCATCCGGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTTCCCGAGGATGGTGGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTGAATGATATTGCTGTTGAGGCAGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTTGTCGTACACCACATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATTCGATCTGGAAAATACCGGGAAATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCGTGTGACATTGAAGCCGGGTGAGAAAAAAAGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGTTGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCTTC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,089 total letters Query= APC115044.159 Length=2541 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3844 0.0 2_A2_APC115044.159Seq171A08.seq 1920 0.0 1_02A_APC115044.159Seq171A02.seq 1330 0.0 V E S L L S K M T L E E K I G Q M N Q I S S Y G N I E D M S Query_1 202 GTAGAATCTCTCCTGTCTAAGATGACCCTTGAGGAGAAAATCGGTCAGATGAACCAGATTTCCTCTTACGGTAATATCGAGGATATGAGT 291 0 1 .......................................................................................... 90 2 1152 ..... 1148 A L I K K G E I G S I L N E V D P V R I N A L Q R V A M E E Query_1 292 GCTTTGATTAAGAAAGGTGAAATCGGTTCCATCTTGAATGAGGTGGATCCGGTGCGTATTAATGCGCTACAGCGCGTGGCAATGGAAGAA 381 0 91 .......................................................................................... 180 2 1147 A...A.N.--........N..-..N--.......-................NN-.......-...NN.................A..... 1066 S R L G I P L L I A R D V I H G F K T I F P I P L G Q A A S Query_1 382 TCCCGTTTGGGTATTCCTTTATTGATAGCGCGTGATGTCATTCACGGGTTTAAAACAATTTTCCCTATTCCCTTGGGACAAGCGGCTTCG 471 0 181 .......................................................................................... 270 2 1065 .T..NG........N.-..........................N.......................NNN.T.--............... 979 F N P Q V A K D G A R I A A I E A S S V G I R W T F A P M I Query_1 472 TTCAATCCGCAGGTAGCGAAAGACGGTGCACGGATAGCAGCTATTGAAGCTTCGTCTGTAGGTATCCGGTGGACTTTTGCGCCAATGATT 561 0 271 .......................................................................................... 360 2 978 ........N.......N...............................................................A......... 889 D I A R D P R W G R I A E G C G E D T Y L T S V M G A A M V Query_1 562 GATATTGCCCGCGATCCTCGCTGGGGACGTATTGCCGAAGGGTGTGGTGAAGATACGTACCTTACTTCCGTAATGGGAGCAGCTATGGTA 651 0 361 .......................................................................................... 450 2 888 .......................................................................................... 799 1 11 ...N.............. 28 E G F Q G D S L N S P T S I A A C P K H F V G Y G A A E G Query_1 652 GAAGGTTTTCAGGGAGATTCGC-TGAATAGTCCTACTTCAATTGCAGCTTGCCCTAAACATTTTGTAGGTTACGGTGCAGCCGAAGGAGG 740 0 451 ......................-................................................................... 539 2 798 ......................-................................................................... 710 1 29 ..........N...........G....N.............................................................. 118 G R D Y N S T F I P E R R L R N V Y L P P F E A A T K A G A Query_1 741 ACGTGATTATAATTCCACGTTCATTCCCGAACGTCGTCTGCGCAATGTTTATTTGCCACCTTTTGAAGCTGCCACCAAAGCGGGTGCAGC 830 0 540 .......................................................................................... 629 2 709 .......................................................................................... 620 1 119 .......................................................................................... 208 A T F M T S F N D N D G I P S T G N A F I L K N V L R D E W Query_1 831 CACGTTTATGACTTCATTTAATGATAATGATGGAATCCCTTCTACCGGGAATGCTTTTATTTTGAAGAATGTACTCCGTGACGAGTGGGG 920 0 630 .......................................................................................... 719 2 619 .......................................................................................... 530 1 209 .......................................................................................... 298 G F D G F V V T D F A S A S E M I S H G F A A G S K E V A M Query_1 921 ATTCGATGGTTTTGTTGTGACGGACTTTGCTTCTGCCAGCGAAATGATAAGCCATGGTTTTGCCGCCGGTTCAAAAGAAGTGGCAATGAA 1010 0 720 ..........................GG.............................................................. 809 2 529 .......................................................................................... 440 2 1080 ........... 1070 1 299 .......................................................................................... 388 K S V N A G V D M E M V S Y T F V K E L P E L V K E G K V K Query_1 1011 ATCTGTGAATGCAGGAGTAGATATGGAAATGGTGAGTTACACTTTTGTGAAGGAACTGCCGGAATTAGTGAAAGAGGGAAAGGTGAAGGA 1100 0 810 .......................................................................................... 899 2 439 .......................................................................................... 350 2 1069 . 1069 1 389 .......................................................................................... 478 E S T I D E A V R N I L R I K Y R L G L F D T P Y V D E Q Q Query_1 1101 AAGCACTATCGATGAGGCTGTTCGTAATATTTTGCGTATAAAGTATCGTTTAGGATTGTTTGATACACCTTATGTAGATGAACAACAAAC 1190 0 900 .......................................................................................... 989 2 349 .......................................................................................... 260 1 479 .......................................................................................... 568 T S V M Y A P S H L E A A K Q A A V E S A I L L K N D K E V Query_1 1191 ATCTGTCATGTATGCTCCTTCTCATTTGGAAGCAGCTAAGCAAGCCGCTGTTGAATCGGCTATTCTGTTGAAGAATGATAAGGAAGTGTT 1280 0 990 .......................................................................................... 1079 2 259 .......................................................................................... 170 1 569 .......................................................................................... 658 L P L Q P S V K T V A V V G P M A N A P Y E Q L G T W I F D Query_1 1281 GCCGTTACAGCCATCTGTGAAAACTGTTGCAGTGGTAGGACCTATGGCTAATGCACCTTATGAACAGTTAGGTACTTGGATATTTGATGG 1370 0 1080 .......................................................................................... 1169 2 169 .......................................................................................... 80 1 659 ............N.....................T..N...............................N.................... 748 G E K A R T Q T P L N A I K E M V G D K V Q V I Y E P G L A Query_1 1371 TGAGAAAGCTCGTACTCAGACTCCGTTGAACGCTATTAAAGAAATGGTTGGCGATAAAGTACAGGTGATTTATGAACCGGGACTAGCATA 1460 0 1170 .......................................................................................... 1259 2 79 ...................................................................... 10 1 749 .................. 766 Y S R E K N P A S V A K A A A A A A R A D V I L A F V G E E Query_1 1461 TAGTCGTGAGAAAAATCCGGCAAGTGTGGCTAAAGCAGCTGCCGCCGCTGCACGTGCAGATGTCATTCTTGCTTTTGTGGGTGAAGAATC 1550 0 1260 .......................................................................................... 1349 0 1318 ............ 1307 S I L S G E A H C L A D L D L Q G D Q G A L I T A L A K T G Query_1 1551 TATTCTTTCGGGTGAAGCTCACTGTTTGGCTGATCTGGATTTGCAGGGTGATCAGGGAGCTTTGATTACAGCTTTGGCTAAGACGGGTAA 1640 0 1350 .......................................................................................... 1439 K P V V T I V M A G R P L T I G K E V E E S T A V L Y S F H Query_1 1641 ACCTGTAGTGACTATTGTGATGGCGGGTCGTCCGTTGACTATCGGTAAAGAAGTCGAAGAGTCGACTGCTGTTCTCTATTCATTCCATCC 1730 0 1440 .......................................................................................... 1529 P G T M G G P A L A D L L W G K A V P S G K A P V T F P R M Query_1 1731 GGGCACAATGGGCGGTCCTGCATTGGCTGATTTGCTTTGGGGGAAGGCTGTGCCGAGTGGAAAGGCGCCGGTCACTTTCCCGAGGATGGT 1820 0 1530 .......................................................................................... 1619 V G Q I P V Y Y A H N N T G R P A T R N E V L L N D I A V E Query_1 1821 GGGACAAATTCCTGTGTACTACGCTCATAATAATACCGGACGTCCGGCTACACGGAATGAAGTGTTGCTGAATGATATTGCTGTTGAGGC 1910 0 1620 .......................................................................................... 1709 0 1666 ............ 1655 A G Q T S L G C T S F Y M D A G F D P L F P F G Y G L S Y T Query_1 1911 AGGACAGACTTCACTGGGCTGTACTTCCTTCTATATGGATGCGGGTTTTGATCCCTTGTTTCCGTTTGGTTATGGCTTGTCGTACACCAC 2000 0 1710 .......................................................................................... 1799 T F K Y S N I K L A S D V L K K D D V L T V T F D L E N T G Query_1 2001 ATTTAAGTATAGCAACATCAAACTGGCGTCTGATGTACTGAAAAAAGATGATGTGCTGACAGTGACATTCGATCTGGAAAATACCGGGAA 2090 0 1800 .......................................................................................... 1889 K Y E G T E V A Q L Y I Q D K I G S V T R P V K E L K R F T Query_1 2091 ATATGAAGGAACGGAAGTAGCTCAATTGTATATACAAGATAAGATTGGTTCCGTGACTCGTCCGGTGAAAGAACTGAAACGCTTCACTCG 2180 0 1890 .......................................................................................... 1979 0 1926 ............ 1915 R V T L K P G E K K S V S F E L P V S E L A F W N I D M A K Query_1 2181 TGTGACATTGAAGCCGGGTGAGaaaaaaaGCGTTTCGTTTGAACTCCCTGTTAGTGAACTTGCATTTTGGAACATAGATATGGCTAAAGT 2270 0 1980 .......................................................................................... 2069 V V E P G D F G L W V A T D S Q S G E E V F F Query_1 2271 TGTGGAACCCGGAGACTTTGGGCTTTGGGTGGCAACGGATAGTCAGTCCGGAGAAGAAGTTTTCTTC 2337 0 2070 ................................................................... 2136 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 10191912 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,089 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115045.148 (APC115045.26-772.P(773).D112A.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVSGEIVTGQAQNSDVAKKIEQGLVGGLLNLKGVEKIRDVQKLAIEKSRLGIPLIFGMDVVHGYETIFPIPLGLSCSWDMEAIRKSARVAAIEASADGISWTFSPMVDISRDPRWGRVSEGNGEDPFLGGAIAKAMVSGYQGIDLNNQLKRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEYMYPYQAAVDAGVGSVMASFNEIDGIPATANKWLMTDVLRKQWGFDGFVVTDFTGISEMIAHGIGDLQTVSARALNAGVDMDMVSEGFTGTIKKSIDEGKISMETLDKACRRILEAKYKLGLFDNPYKYCDLKRPKRDIFTKEHRDAARKIAGESFVLLKNDKSGSSANPTLPLKKEGTVAVIGPLANTRSNMPGTWSVAARLNDYPSVYEGLKEMMKGKVNITYAKGSNLISDAAYEERATMFGRSLNRDNRTDKEMLDEALKVAANADVIIAALGESSEMSGESSSRTNLALPDVQRTLLEALLKTGKPVVLTLFTGRPLTLTWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLLEGKWFEKFRSNYLDVDNDPLYPFGYGLSYTNFQYSDITLSAPTMGQDGSVTAMVTVTNTGKYDGAEVVQLYIRDLVGSITRPVKELKGFDKIFLKAGESKTVSFKITPELLRFYDYELNYVAEPGDFDIMIGGNSQSVKTTHLSL
AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTTTCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAAGGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCATGGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATCGAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAACGGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAACGATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAACCGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGCATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGTATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGTGAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAAGCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGCGACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAAAAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGACTATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCAGCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,493 total letters Query= APC115045.148 Length=2646 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4033 0.0 2_B2_APC115045.148Seq171B08.seq 1878 0.0 1_02B_B2_APC115045.148_T7S.seq 1775 0.0 Q F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 55 CAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTG 144 1 4 ........-................................................................................. 92 W S H P Q F E K G T E N L Y F Q S N A K S P Q D M D R F I D Query_1 145 TGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCAAGTCTCCGCAGGACATGGATCGCTTCATCGAT 234 0 1 ................................. 33 1 93 .......................................................................................... 182 A L M K K M T V E E K I G Q L N L P V S G E I V T G Q A Q N Query_1 235 GCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTTTCCGGCGAGATCGTCACCGGGCAGGCACAAAAC 324 0 34 .......................................................................................... 123 1 183 .......................................................................................... 272 S D V A K K I E Q G L V G G L L N L K G V E K I R D V Q K L Query_1 325 AGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAAGGGGTGGAGAAGATACGCGATGTACAAAAACTG 414 0 124 .......................................................................................... 213 1 273 .......................................................................................... 362 A I E K S R L G I P L I F G M A V V H G Y E T I F P I P L G Query_1 415 GCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGCTGTAGTGCATGGTTACGAAACCATTTTCCCTATTCCATTAGGC 504 0 214 ..............................................AC.......................................... 303 1 363 .......................................................................................... 452 L S C S W D M E A I R K S A R V A A I E A S A D G I S W T F Query_1 505 CTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATCGAGGCCAGTGCTGATGGTATTTCCTGGACATTC 594 0 304 .......................................................................................... 393 1 453 .......................................................................................... 542 S P M V D I S R D P R W G R V S E G N G E D P F L G G A I Query_1 595 AGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCA--GCGAGGGTAACGGCGAAGACCCATTCTTGGGTGGAGCCAT-C 681 0 394 ..............................................--........................................-. 480 2 1198 ...T.....NN-...T...N.-.....T..N-.NNA........TNAG............NNANNN---........N...-......T. 1116 1 543 ..............................................--........................................-. 629 A K A M V S G Y Q G I D L N N Q L K R N D E I M A C V K H F Query_1 682 GCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAACGATGAAATTATGGCATGTGTAAAGCACTTC 771 0 481 .......................................................................................... 570 2 1115 ..N.N-......-......T.AT.....-.......-...C..NN-......N.NC--..............-....N....-....... 1035 1 630 .......................................................................................... 719 A L Y G A G E A G R D Y N T V D M S R N R M F N E Y M Y P Query_1 772 GCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAACCGTATGTTCAACGAATACATGTATCCCT-A 860 0 571 ........................................................................................-. 659 2 1034 ..NNGT...........NN.-...................................................................T. 946 1 720 ........................................................................................-. 808 Y Q A A V D A G V G S V M A S F N E I D G I P A T A N K W L Query_1 861 CCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTC-TTTCAACGAAATAGACGGCATACCAGCCACGGCCAATAAATGGC-TG 948 0 660 ..........................................-............................................-.. 747 2 945 ..........................................-............................................-.. 858 1 809 .....................................T....T............................................T.. 898 M T D V L R K Q W G F D G F V V T D F T G I S E M I A H G Query_1 949 ATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGTATCTCCGAAAT-GATAGCGCACGGCAT 1037 0 748 ..........................................................................-............... 836 2 857 ..........................................................................-............... 769 1 899 ..........................................................................N............... 988 I G D L Q T V S A R A L N A G V D M D M V S E G F T G T I Query_1 1038 CGGTGAC-TTGC-AGACT--GTTTCCGCACGTGCACT-CAATGCAGGCGTGGATATGGACATGGTAAGTGAAGGCTTCACGGGTACAATC 1122 0 837 .......-....-.....--.................-.................................................... 921 2 768 .......-....-.....--.................-.................................................... 684 1 989 .......T....A.N...TGT.......N........N.......T.. 1036 K K S I D E G K I S M E T L D K A C R R I L E A K Y K L G L Query_1 1123 AAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAAGCCAAATACAAACTGGGATTA 1212 0 922 .......................................................................................... 1011 2 683 .......................................................................................... 594 F D N P Y K Y C D L K R P K R D I F T K E H R D A A R K I A Query_1 1213 TTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGCGACGCTGCTCGTAAGATTGCG 1302 0 1012 .......................................................................................... 1101 2 593 .......................................................................................... 504 G E S F V L L K N D K S G S S A N P T L P L K K E G T V A V Query_1 1303 GGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAAAAAGAAGGTACGGTGGCTGTC 1392 0 1102 .......................................................................................... 1191 2 503 .......................................................................................... 414 I G P L A N T R S N M P G T W S V A A R L N D Y P S V Y E G Query_1 1393 ATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGACTATCCTTCTGTGTACGAAGGA 1482 0 1192 .......................................................................................... 1281 2 413 .......................................................................................... 324 L K E M M K G K V N I T Y A K G S N L I S D A A Y E E R A T Query_1 1483 TTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCAGCCTACGAAGAACGTGCCACA 1572 0 1282 .......................................................................................... 1371 2 323 .......................................................................................... 234 M F G R S L N R D N R T D K E M L D E A L K V A A N A D V I Query_1 1573 ATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTGGCCGCTAATGCAGATGTAATA 1662 0 1372 .......................................................................................... 1461 2 233 .......................................................................................... 144 I A A L G E S S E M S G E S S S R T N L A L P D V Q R T L L Query_1 1663 ATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCCGATGTACAGCGCACTCTATTG 1752 0 1462 .......................................................................................... 1551 2 143 .......................................................................................... 54 E A L L K T G K P V V L T L F T G R P L T L T W E Q E H V P Query_1 1753 GAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACTTGGGAACAGGAGCATGTGCCC 1842 0 1552 .......................................................................................... 1641 2 53 ............................................ 10 A I L N V W F G G S E A A Y A I G D V L F G D V N P S G K L Query_1 1843 GCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGATGTAAATCCCAGCGGCAAACTA 1932 0 1642 .......................................................................................... 1731 T M T F P K N V G Q I P L F Y N H K N T G R P L L E G K W F Query_1 1933 ACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCTTTACTTGAAGGCAAATGGTTC 2022 0 1732 .......................................................................................... 1821 E K F R S N Y L D V D N D P L Y P F G Y G L S Y T N F Q Y S Query_1 2023 GAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCGTATACCAACTTTCAATACAGC 2112 0 1822 .......................................................................................... 1911 D I T L S A P T M G Q D G S V T A M V T V T N T G K Y D G A Query_1 2113 GACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCA 2202 0 1912 .......................................................................................... 2001 E V V Q L Y I R D L V G S I T R P V K E L K G F D K I F L K Query_1 2203 GAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAA 2292 0 2002 .......................................................................................... 2091 A G E S K T V S F K I T P E L L R F Y D Y E L N Y V A E P G Query_1 2293 GCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGA 2382 0 2092 .......................................................................................... 2181 D F D I M I G G N S Q S V K T T H L S L Query_1 2383 GACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG 2442 0 2182 ............................................................ 2241 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11678018 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,493 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115045.149 (APC115045.26-772.P(773).K213A.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVSGEIVTGQAQNSDVAKKIEQGLVGGLLNLKGVEKIRDVQKLAIEKSRLGIPLIFGMDVVHGYETIFPIPLGLSCSWDMEAIRKSARVAAIEASADGISWTFSPMVDISRDPRWGRVSEGNGEDPFLGGAIAKAMVSGYQGIDLNNQLKRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEYMYPYQAAVDAGVGSVMASFNEIDGIPATANKWLMTDVLRKQWGFDGFVVTDFTGISEMIAHGIGDLQTVSARALNAGVDMDMVSEGFTGTIKKSIDEGKISMETLDKACRRILEAKYKLGLFDNPYKYCDLKRPKRDIFTKEHRDAARKIAGESFVLLKNDKSGSSANPTLPLKKEGTVAVIGPLANTRSNMPGTWSVAARLNDYPSVYEGLKEMMKGKVNITYAKGSNLISDAAYEERATMFGRSLNRDNRTDKEMLDEALKVAANADVIIAALGESSEMSGESSSRTNLALPDVQRTLLEALLKTGKPVVLTLFTGRPLTLTWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLLEGKWFEKFRSNYLDVDNDPLYPFGYGLSYTNFQYSDITLSAPTMGQDGSVTAMVTVTNTGKYDGAEVVQLYIRDLVGSITRPVKELKGFDKIFLKAGESKTVSFKITPELLRFYDYELNYVAEPGDFDIMIGGNSQSVKTTHLSL
AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTTTCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAAGGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCATGGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATCGAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAACGGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAACGATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAACCGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGCATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGTATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGTGAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAAGCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGCGACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAAAAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGACTATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCAGCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,595 total letters Query= APC115045.149 Length=2646 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4028 0.0 1_02C_APC115045.149Seq171C02.seq 1801 0.0 2_C2_APC115045.149Seq171C08.seq 1692 0.0 K S P Q D M D R F I D A L M K K M T V E E K I G Q L N L P V Query_1 202 AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTT 291 0 1 .......................................................................................... 90 S G E I V T G Q A Q N S D V A K K I E Q G L V G G L L N L K Query_1 292 TCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAA 381 0 91 .......................................................................................... 180 G V E K I R D V Q K L A I E K S R L G I P L I F G M D V V H Query_1 382 GGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCAT 471 0 181 .......................................................................................... 270 G Y E T I F P I P L G L S C S W D M E A I R K S A R V A A I Query_1 472 GGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATC 561 0 271 .......................................................................................... 360 E A S A D G I S W T F S P M V D I S R D P R W G R V S E G N Query_1 562 GAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAAC 651 0 361 .......................................................................................... 450 G E D P F L G G A I A K A M V S G Y Q G I D L N N Q L K R Query_1 652 GGCGAAGACCCATTCTTGGG-TGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAA 740 0 451 ....................-..................................................................... 539 1 17 .....N...T.N............................................................... 91 2 1085 .....NC. 1078 N D E I M A C V A H F A L Y G A G E A G R D Y N T V D Query_1 741 CGA-TGAAATTATGGC-ATGTGTAGCTC-ACTTCG-CACTGT-AT-GGTGCCGGAG-AAGCCGGA--CGTGATT-ACAATACCGTAGATA 820 0 540 ...-............-.......AAG.-......-......-..-..........-........--.......-............... 619 1 92 ...-............-...........-......-......-..-..........-........--.......-............... 171 2 1077 ...G............C.NN.....N..C....N.N...N..T..N..........G.....C..NA.......T...G...N....... 988 M S R N R M F N E Y M Y P Y Q A A V D A G V G S V M A S F Query_1 821 T-GAGTCGTAACCGT-ATGTTCAACGAATACATGTATCCCTACCAAGCT-GCC-GTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTC 906 0 620 .-.............-.................................-...-.................................... 705 1 172 .-.............-.................................-...-.................................... 257 2 987 .T.............N.....NG.........N..........-.....N...N...........................N-..N.... 900 N E I D G I P A T A N K W L M T D V L R K Q W G F D G F V Query_1 907 AACGAAATAGACGGCATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAA-TGGGGCTTCGACGGCTTTGTGGT 995 0 706 ..................................................................-....................... 794 1 258 ..................................................................-....................... 346 2 899 .N-..........................-.........N.....N........N...........A........G.....GC..N.... 812 V T D F T G I S E M I A H G I G D L Q T V S A R A L N A G V Query_1 996 GACAGACTTTACCGGTATCTCCGAAA-TGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGG 1084 0 795 ..........................-............................................................... 883 1 347 ..........................-............................................................... 435 2 811 .....................N....A............................................................... 722 D M D M V S E G F T G T I K K S I D E G K I S M E T L D K A Query_1 1085 ATATGGACATGGTAAGTGAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCT 1174 0 884 .......................................................................................... 973 1 436 .......................................................................................... 525 2 721 .......................................................................................... 632 C R R I L E A K Y K L G L F D N P Y K Y C D L K R P K R D I Query_1 1175 GTCGCCGCATCCTTGAAGCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCT 1264 0 974 .......................................................................................... 1063 1 526 .......................................................................................... 615 2 631 .......................................................................................... 542 F T K E H R D A A R K I A G E S F V L L K N D K S G S S A N Query_1 1265 TCACCAAGGAACATCGCGACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACC 1354 0 1064 .......................................................................................... 1153 1 616 .......................................................................................... 705 2 541 .......................................................................................... 452 P T L P L K K E G T V A V I G P L A N T R S N M P G T W S V Query_1 1355 CAACACTTCCTTTGAAAAAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAG 1444 0 1154 .......................................................................................... 1243 1 706 .......................................................................................... 795 2 451 .......................................................................................... 362 A A R L N D Y P S V Y E G L K E M M K G K V N I T Y A K G S Query_1 1445 CCGCACGCCTCAACGACTATCCTTCTGTGTACGAAGGATTGA-AAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGT 1533 0 1244 ..........................................-............................................... 1332 1 796 ..........................................T............................................... 885 2 361 ..........................................-............................................... 273 N L I S D A A Y E E R A T M F G R S L N R D N R T D K E M L Query_1 1534 AACCTCATCAGTGATGCAGCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTG 1623 0 1333 .......................................................................................... 1422 1 886 -.......N....................................................N.....................NN..... 974 2 272 .......................................................................................... 183 D E A L K V A A N A D V I I A A L G E S S E M S G E S S Query_1 1624 GATG-AGGCGCTGAAAGTGGCCGCTAATGCAGATGTAATAAT-AGCCGCATTGGGAGAATCATCTGAAATG-AGTGGTGAAA-GTTCAAG 1709 0 1423 ....-.....................................-............................-..........-....... 1508 1 975 ....A.................N...................N....N.-.........N...........A..........G..C..N. 1063 2 182 ....-.....................................-............................-..........-....... 97 S R T N L A L P D V Q R T L L E A L L K T G K P V V L T L Query_1 1710 CCG-CACTAAC-CTGGCTCTTCCCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCT-CTT 1796 0 1509 ...-.......-..........................................................................-... 1595 1 1064 ...C.......T...NNC...T.... 1089 2 96 ...-.......-.............................................................-............N... 10 F T G R P L T L T W E Q E H V P A I L N V W F G G S E A A Y Query_1 1797 TACAGGTCGCCCACTAACGTTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGC 1886 0 1596 .......................................................................................... 1685 2 9 A....... 2 A I G D V L F G D V N P S G K L T M T F P K N V G Q I P L F Query_1 1887 CATTGGCGATGTATTGTTCGGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTA 1976 0 1686 .......................................................................................... 1775 Y N H K N T G R P L L E G K W F E K F R S N Y L D V D N D P Query_1 1977 CAATCATAAAAATACCGGTCGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATT 2066 0 1776 .......................................................................................... 1865 L Y P F G Y G L S Y T N F Q Y S D I T L S A P T M G Q D G S Query_1 2067 GTATCCATTCGGCTATGGTTTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGT 2156 0 1866 .......................................................................................... 1955 V T A M V T V T N T G K Y D G A E V V Q L Y I R D L V G S I Query_1 2157 TACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCAC 2246 0 1956 .......................................................................................... 2045 T R P V K E L K G F D K I F L K A G E S K T V S F K I T P E Query_1 2247 CCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATT 2336 0 2046 .......................................................................................... 2135 L L R F Y D Y E L N Y V A E P G D F D I M I G G N S Q S V K Query_1 2337 ACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAAC 2426 0 2136 .......................................................................................... 2225 T T H L S L Query_1 2427 GACTCATCTGAGTTTG 2442 0 2226 ................ 2241 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11946176 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,595 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115045.150 (APC115045.26-772.P(773).H214A.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVSGEIVTGQAQNSDVAKKIEQGLVGGLLNLKGVEKIRDVQKLAIEKSRLGIPLIFGMDVVHGYETIFPIPLGLSCSWDMEAIRKSARVAAIEASADGISWTFSPMVDISRDPRWGRVSEGNGEDPFLGGAIAKAMVSGYQGIDLNNQLKRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEYMYPYQAAVDAGVGSVMASFNEIDGIPATANKWLMTDVLRKQWGFDGFVVTDFTGISEMIAHGIGDLQTVSARALNAGVDMDMVSEGFTGTIKKSIDEGKISMETLDKACRRILEAKYKLGLFDNPYKYCDLKRPKRDIFTKEHRDAARKIAGESFVLLKNDKSGSSANPTLPLKKEGTVAVIGPLANTRSNMPGTWSVAARLNDYPSVYEGLKEMMKGKVNITYAKGSNLISDAAYEERATMFGRSLNRDNRTDKEMLDEALKVAANADVIIAALGESSEMSGESSSRTNLALPDVQRTLLEALLKTGKPVVLTLFTGRPLTLTWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLLEGKWFEKFRSNYLDVDNDPLYPFGYGLSYTNFQYSDITLSAPTMGQDGSVTAMVTVTNTGKYDGAEVVQLYIRDLVGSITRPVKELKGFDKIFLKAGESKTVSFKITPELLRFYDYELNYVAEPGDFDIMIGGNSQSVKTTHLSL
AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTTTCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAAGGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCATGGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATCGAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAACGGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAACGATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAACCGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGCATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGTATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGTGAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAAGCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGCGACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAAAAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGACTATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCAGCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,713 total letters Query= APC115045.150 Length=2646 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4028 0.0 1_02D_APC115045.150Seq171D02.seq 1966 0.0 2_D2_APC115045.150Seq171D08.seq 1948 0.0 K S P Q D M D R F I D A L M K K M T V E E K I G Q L N L P V Query_1 202 AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTT 291 0 1 .......................................................................................... 90 S G E I V T G Q A Q N S D V A K K I E Q G L V G G L L N L K Query_1 292 TCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAA 381 0 91 .......................................................................................... 180 G V E K I R D V Q K L A I E K S R L G I P L I F G M D V V H Query_1 382 GGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCAT 471 0 181 .......................................................................................... 270 G Y E T I F P I P L G L S C S W D M E A I R K S A R V A A I Query_1 472 GGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATC 561 0 271 .......................................................................................... 360 E A S A D G I S W T F S P M V D I S R D P R W G R V S E G Query_1 562 GAGGCCAGTGCTGATGGTATTTCC-TGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAA 650 0 361 ........................-................................................................. 449 2 1220 .....N...NNN.....N......T.GA..T...NNAN....N.CNCN....NNN.....NNNC....... 1150 N G E D P F L G G A I A K A M V S G Y Q G I D L N N Q L K R Query_1 651 CGGCGAAGACCCATTCTTGGGTGGAGCCAT-CGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCA 739 0 450 ..............................-........................................................... 538 1 12 .................-........................................................... 87 2 1149 ...............TC...-.N.NN....T..........T..N.--...T.AT.....TNT.....T...C..N.--........... 1065 N D E I M A C V K A F A L Y G A G E A G R D Y N T V D M S Query_1 740 ACGATGAAATTATGGCATG-TGTAAAGGCTTTCGC-ACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCG 827 0 539 ...................-.......CAC.....-...................................................... 626 1 88 ...................-...............-...................................................... 175 2 1064 ...................N...............C.N......N..............N.............................. 975 R N R M F N E Y M Y P Y Q A A V D A G V G S V M A S F N E I Query_1 828 TAACCGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGA 917 0 627 .......................................................................................... 716 1 176 ...................N...................................................................... 265 2 974 ..-...........................-........................................................... 887 D G I P A T A N K W L M T D V L R K Q W G F D G F V V T D F Query_1 918 CGGCATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTAC 1007 0 717 .......................................................................................... 806 1 266 .......................................................................................... 355 2 886 .......................................................................................... 797 T G I S E M I A H G I G D L Q T V S A R A L N A G V D M D M Query_1 1008 CGGTATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGT 1097 0 807 .......................................................................................... 896 1 356 .......................................................................................... 445 2 796 .......................................................................................... 707 V S E G F T G T I K K S I D E G K I S M E T L D K A C R R I Query_1 1098 AAGTGAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCT 1187 0 897 .......................................................................................... 986 1 446 .......................................................................................... 535 2 706 .......................................................................................... 617 L E A K Y K L G L F D N P Y K Y C D L K R P K R D I F T K E Query_1 1188 TGAAGCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACA 1277 0 987 .......................................................................................... 1076 1 536 .......................................................................................... 625 2 616 .......................................................................................... 527 H R D A A R K I A G E S F V L L K N D K S G S S A N P T L P Query_1 1278 TCGCGACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTT 1367 0 1077 .......................................................................................... 1166 1 626 .......................................................................................... 715 2 526 .......................................................................................... 437 L K K E G T V A V I G P L A N T R S N M P G T W S V A A R L Query_1 1368 GAAAAAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAA 1457 0 1167 .......................................................................................... 1256 1 716 .......................................................................................... 805 2 436 .......................................................................................... 347 N D Y P S V Y E G L K E M M K G K V N I T Y A K G S N L I S Query_1 1458 CGACTATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGA 1547 0 1257 .......................................................................................... 1346 1 806 .......................................................................................... 895 2 346 .......................................................................................... 257 D A A Y E E R A T M F G R S L N R D N R T D K E M L D E A L Query_1 1548 TGCAGCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAA 1637 0 1347 .......................................................................................... 1436 1 896 .......................................................................................... 985 2 256 .......................................................................................... 167 K V A A N A D V I I A A L G E S S E M S G E S S S R T N L A Query_1 1638 AGTGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCT 1727 0 1437 .......................................................................................... 1526 1 986 .....N...............-.N................N.....................-......................CT... 1073 2 166 .......................................................................................... 77 L P D V Q R T L L E A L L K T G K P V V L T L F T G R P L Query_1 1728 TCCCGAT-GTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGT--CGCCCACTAAC 1814 0 1527 .......-.....................................................................--........... 1613 1 1074 .-.....G..........N.....--.G.N................N......N.--NN.........-..NN....TCN.......... 1157 2 76 .......-.............................................................. 8 T L T W E Q E H V P A I L N V W F G G S E A A Y A I G D V L Query_1 1815 GTTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTT 1904 0 1614 .......................................................................................... 1703 1 1158 .......G..A...N.... 1176 F G D V N P S G K L T M T F P K N V G Q I P L F Y N H K N T Query_1 1905 CGGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGG 1994 0 1704 .......................................................................................... 1793 G R P L L E G K W F E K F R S N Y L D V D N D P L Y P F G Y Query_1 1995 TCGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGG 2084 0 1794 .......................................................................................... 1883 G L S Y T N F Q Y S D I T L S A P T M G Q D G S V T A M V T Query_1 2085 TTTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGT 2174 0 1884 .......................................................................................... 1973 V T N T G K Y D G A E V V Q L Y I R D L V G S I T R P V K E Query_1 2175 AACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACT 2264 0 1974 .......................................................................................... 2063 L K G F D K I F L K A G E S K T V S F K I T P E L L R F Y D Query_1 2265 GAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTA 2354 0 2064 .......................................................................................... 2153 Y E L N Y V A E P G D F D I M I G G N S Q S V K T T H L S L Query_1 2355 TGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG 2442 0 2154 ........................................................................................ 2241 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12256398 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,713 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115045.151 (APC115045.26-772.P(773).F635A.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVSGEIVTGQAQNSDVAKKIEQGLVGGLLNLKGVEKIRDVQKLAIEKSRLGIPLIFGMDVVHGYETIFPIPLGLSCSWDMEAIRKSARVAAIEASADGISWTFSPMVDISRDPRWGRVSEGNGEDPFLGGAIAKAMVSGYQGIDLNNQLKRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEYMYPYQAAVDAGVGSVMASFNEIDGIPATANKWLMTDVLRKQWGFDGFVVTDFTGISEMIAHGIGDLQTVSARALNAGVDMDMVSEGFTGTIKKSIDEGKISMETLDKACRRILEAKYKLGLFDNPYKYCDLKRPKRDIFTKEHRDAARKIAGESFVLLKNDKSGSSANPTLPLKKEGTVAVIGPLANTRSNMPGTWSVAARLNDYPSVYEGLKEMMKGKVNITYAKGSNLISDAAYEERATMFGRSLNRDNRTDKEMLDEALKVAANADVIIAALGESSEMSGESSSRTNLALPDVQRTLLEALLKTGKPVVLTLFTGRPLTLTWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLLEGKWFEKFRSNYLDVDNDPLYPFGYGLSYTNFQYSDITLSAPTMGQDGSVTAMVTVTNTGKYDGAEVVQLYIRDLVGSITRPVKELKGFDKIFLKAGESKTVSFKITPELLRFYDYELNYVAEPGDFDIMIGGNSQSVKTTHLSL
AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTTTCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAAGGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCATGGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATCGAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAACGGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAACGATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAACCGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGCATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGTATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGTGAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAAGCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGCGACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAAAAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGACTATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCAGCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,302 total letters Query= APC115045.151 Length=2646 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4028 0.0 1_02E_APC115045.151Seq171E02.seq 1770 0.0 2_11E_E2_APC115045.151_T7term.seq 1633 0.0 K S P Q D M D R F I D A L M K K M T V E E K I G Q L N L P V Query_1 202 AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTT 291 0 1 .......................................................................................... 90 S G E I V T G Q A Q N S D V A K K I E Q G L V G G L L N L K Query_1 292 TCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAA 381 0 91 .......................................................................................... 180 G V E K I R D V Q K L A I E K S R L G I P L I F G M D V V H Query_1 382 GGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCAT 471 0 181 .......................................................................................... 270 G Y E T I F P I P L G L S C S W D M E A I R K S A R V A A I Query_1 472 GGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATC 561 0 271 .......................................................................................... 360 E A S A D G I S W T F S P M V D I S R D P R W G R V S E G N Query_1 562 GAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAAC 651 0 361 .......................................................................................... 450 G E D P F L G G A I A K A M V S G Y Q G I D L N N Q L K R N Query_1 652 GGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAAC 741 0 451 .......................................................................................... 540 D E I M A C V K H F A L Y G A G E A G R D Y N T V D M S R N Query_1 742 GATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAAC 831 0 541 .......................................................................................... 630 R M F N E Y M Y P Y Q A A V D A G V G S V M A S F N E I D G Query_1 832 CGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGC 921 0 631 .......................................................................................... 720 I P A T A N K W L M T D V L R K Q W G F D G F V V T D F T G Query_1 922 ATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGT 1011 0 721 .......................................................................................... 810 I S E M I A H G I G D L Q T V S A R A L N A G V D M D M V S Query_1 1012 ATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGT 1101 0 811 .......................................................................................... 900 E G F T G T I K K S I D E G K I S M E T L D K A C R R I L E Query_1 1102 GAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAA 1191 0 901 .......................................................................................... 990 A K Y K L G L F D N P Y K Y C D L K R P K R D I F T K E H R Query_1 1192 GCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGC 1281 0 991 .......................................................................................... 1080 D A A R K I A G E S F V L L K N D K S G S S A N P T L P L K Query_1 1282 GACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAA 1371 0 1081 .......................................................................................... 1170 K E G T V A V I G P L A N T R S N M P G T W S V A A R L N D Query_1 1372 AAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGAC 1461 0 1171 .......................................................................................... 1260 Y P S V Y E G L K E M M K G K V N I T Y A K G S N L I S D A Query_1 1462 TATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCA 1551 0 1261 .......................................................................................... 1350 A Y E E R A T M F G R S L N R D N R T D K E M L D E A L K Query_1 1552 GCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAA-GT 1640 0 1351 .......................................................................................-.. 1439 1 2 ......N-..............................-.. 40 2 960 .....NNT........NN........A.. 932 V A A N A D V I I A A L G E S S E M S G E S S S R T N L A L Query_1 1641 GGCCGCTAATGCAGATGTAATAATA-GCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTC 1729 0 1440 .........................-................................................................ 1528 1 41 .........................C................................................................ 130 2 931 .N...N...................-......N...N............................A............T........... 843 P D V Q R T L L E A L L K T G K P V V L T L F T G R P L T L Query_1 1730 CCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGA 1819 0 1529 .......................................................................................... 1618 1 131 .......................................................................................... 220 2 842 ..........................................A...N....................N...................... 753 T W E Q E H V P A I L N V W F G G S E A A Y A I G D V L F G Query_1 1820 CTTGGGAACAGGAGCATGTGCCCGCCATCCTGAA-TGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGC 1908 0 1619 ..................................-....................................................... 1707 1 221 ..................................-....................................................... 309 2 752 ..................................A....................................................... 663 D V N P S G K L T M T F P K N V G Q I P L F Y N H K N T G R Query_1 1909 GATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGT 1998 0 1708 .......................................................................................... 1797 1 310 .......................................................................................... 399 2 662 .......................................................................................... 573 P L L E G K W F E K A R S N Y L D V D N D P L Y P F G Y G L Query_1 1999 CCTTTACTTGAAGGCAAATGGTTCGAAAAAGCTCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTG 2088 0 1798 ..............................TTC......................................................... 1887 1 400 .......................................................................................... 489 2 572 .......................................................................................... 483 S Y T N F Q Y S D I T L S A P T M G Q D G S V T A M V T V T Query_1 2089 TCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACC 2178 0 1888 .......................................................................................... 1977 1 490 .......................................................................................... 579 2 482 .......................................................................................... 393 N T G K Y D G A E V V Q L Y I R D L V G S I T R P V K E L K Query_1 2179 AATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAA 2268 0 1978 .......................................................................................... 2067 1 580 .......................................................................................... 669 2 392 .......................................................................................... 303 G F D K I F L K A G E S K T V S F K I T P E L L R F Y D Y E Query_1 2269 GGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAA 2358 0 2068 .......................................................................................... 2157 1 670 .......................................................................................... 759 2 302 .......................................................................................... 213 L N Y V A E P G D F D I M I G G N S Q S V K T T H L S L * H Query_1 2359 CTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTGTAACAT 2448 0 2158 .................................................................................... 2241 1 760 ...G..................................................--.................................. 847 2 212 ...G...................................................................................... 123 W K W I T D P N S S S V D K L A A A L E H H H H H H * D P A Query_1 2449 TGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCT 2538 1 848 ....................N.....C................................N........-..................... 936 2 122 .......................................................................................... 33 A N K A R K E A E L A A A T A E Q * L A * P L G A S K R V Query_1 2539 GCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC-TAGCATAACCCCTTGGGGCCTCTAAACGGGTCTT 2627 1 937 ....T.....................-N..........G................T...N..-..............T....N....... 1024 2 32 ..................A.N.. 10 L R G F L L K Query_1 2628 GAGGGGTTTTTTGCTGAAA 2646 1 1025 .NA...G.N.......... 1043 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11175879 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,302 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115045.152 (APC115045.26-772.P(773).W437F.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVSGEIVTGQAQNSDVAKKIEQGLVGGLLNLKGVEKIRDVQKLAIEKSRLGIPLIFGMDVVHGYETIFPIPLGLSCSWDMEAIRKSARVAAIEASADGISWTFSPMVDISRDPRWGRVSEGNGEDPFLGGAIAKAMVSGYQGIDLNNQLKRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEYMYPYQAAVDAGVGSVMASFNEIDGIPATANKWLMTDVLRKQWGFDGFVVTDFTGISEMIAHGIGDLQTVSARALNAGVDMDMVSEGFTGTIKKSIDEGKISMETLDKACRRILEAKYKLGLFDNPYKYCDLKRPKRDIFTKEHRDAARKIAGESFVLLKNDKSGSSANPTLPLKKEGTVAVIGPLANTRSNMPGTWSVAARLNDYPSVYEGLKEMMKGKVNITYAKGSNLISDAAYEERATMFGRSLNRDNRTDKEMLDEALKVAANADVIIAALGESSEMSGESSSRTNLALPDVQRTLLEALLKTGKPVVLTLFTGRPLTLTWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLLEGKWFEKFRSNYLDVDNDPLYPFGYGLSYTNFQYSDITLSAPTMGQDGSVTAMVTVTNTGKYDGAEVVQLYIRDLVGSITRPVKELKGFDKIFLKAGESKTVSFKITPELLRFYDYELNYVAEPGDFDIMIGGNSQSVKTTHLSL
AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTTTCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAAGGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCATGGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATCGAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAACGGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAACGATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAACCGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGCATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGTATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGTGAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAAGCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGCGACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAAAAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGACTATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCAGCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,669 total letters Query= APC115045.152 Length=2646 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4033 0.0 1_02F_APC115045.152Seq171F02.seq 1880 0.0 2_F2_APC115045.152Seq171F08.seq 1858 0.0 K S P Q D M D R F I D A L M K K M T V E E K I G Q L N L P V Query_1 202 AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTT 291 0 1 .......................................................................................... 90 S G E I V T G Q A Q N S D V A K K I E Q G L V G G L L N L K Query_1 292 TCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAA 381 0 91 .......................................................................................... 180 2 1117 .....- 1113 G V E K I R D V Q K L A I E K S R L G I P L I F G M D V V H Query_1 382 GGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCAT 471 0 181 .......................................................................................... 270 2 1112 -............NN........N-........................N.-..........N-......-..............N.... 1028 G Y E T I F P I P L G L S C S W D M E A I R K S A R V A A I Query_1 472 GGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATC 561 0 271 .......................................................................................... 360 2 1027 ...-.....NNN....G....N.N.-.....-...N........N...-.....AG.................................. 942 E A S A D G I S W T F S P M V D I S R D P R W G R V S E G N Query_1 562 GAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAAC 651 0 361 .......................................................................................... 450 2 941 ..........................-..........-.................................................... 854 G E D P F L G G A I A K A M V S G Y Q G I D L N N Q L K R N Query_1 652 GGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAAC 741 0 451 .......................................................................................... 540 2 853 .......................................................................................... 764 D E I M A C V K H F A L Y G A G E A G R D Y N T V D M S R N Query_1 742 GATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAAC 831 0 541 .......................................................................................... 630 2 763 .......................................................................................... 674 R M F N E Y M Y P Y Q A A V D A G V G S V M A S F N E I D G Query_1 832 CGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGC 921 0 631 .......................................................................................... 720 2 673 .......................................................................................... 584 I P A T A N K W L M T D V L R K Q W G F D G F V V T D F T G Query_1 922 ATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGT 1011 0 721 .......................................................................................... 810 2 583 .......................................................................................... 494 I S E M I A H G I G D L Q T V S A R A L N A G V D M D M V S Query_1 1012 ATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGT 1101 0 811 .......................................................................................... 900 2 493 .......................................................................................... 404 E G F T G T I K K S I D E G K I S M E T L D K A C R R I L E Query_1 1102 GAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAA 1191 0 901 .......................................................................................... 990 2 403 .......................................................................................... 314 A K Y K L G L F D N P Y K Y C D L K R P K R D I F T K E H R Query_1 1192 GCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGC 1281 0 991 .......................................................................................... 1080 2 313 .......................................................................................... 224 D A A R K I A G E S F V L L K N D K S G S S A N P T L P L K Query_1 1282 GACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAA 1371 0 1081 .......................................................................................... 1170 1 2 ..........................-........................................... 70 2 223 .......................................................................................... 134 K E G T V A V I G P L A N T R S N M P G T F S V A A R L N D Query_1 1372 AAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTTTAGTGTAGCCGCACGCCTCAACGAC 1461 0 1171 ................................................................GG........................ 1260 1 71 .......................................................................................... 160 2 133 .......................................................................................... 44 Y P S V Y E G L K E M M K G K V N I T Y A K G S N L I S D A Query_1 1462 TATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCA 1551 0 1261 .......................................................................................... 1350 1 161 .......................................................................................... 250 2 43 .....................-............ 11 A Y E E R A T M F G R S L N R D N R T D K E M L D E A L K V Query_1 1552 GCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTG 1641 0 1351 .......................................................................................... 1440 1 251 .......................................................................................... 340 A A N A D V I I A A L G E S S E M S G E S S S R T N L A L P Query_1 1642 GCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCC 1731 0 1441 .......................................................................................... 1530 1 341 .......................................................................................... 430 D V Q R T L L E A L L K T G K P V V L T L F T G R P L T L T Query_1 1732 GATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACT 1821 0 1531 .......................................................................................... 1620 1 431 .......................................................................................... 520 W E Q E H V P A I L N V W F G G S E A A Y A I G D V L F G D Query_1 1822 TGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGAT 1911 0 1621 .......................................................................................... 1710 1 521 .......................................................................................... 610 V N P S G K L T M T F P K N V G Q I P L F Y N H K N T G R P Query_1 1912 GTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCT 2001 0 1711 .......................................................................................... 1800 1 611 .......................................................................................... 700 L L E G K W F E K F R S N Y L D V D N D P L Y P F G Y G L Query_1 2002 TTACTTG-AAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTC 2090 0 1801 .......-.................................................................................. 1889 1 701 .......A.................................................................................. 790 S Y T N F Q Y S D I T L S A P T M G Q D G S V T A M V T V T Query_1 2091 GTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAA 2180 0 1890 .......................................................................................... 1979 1 791 .......................................................................................... 880 N T G K Y D G A E V V Q L Y I R D L V G S I T R P V K E L K Query_1 2181 TACCGGTAAGTACGATGGTGCAGAAGTAGTGCAAC-TTTATATCCGTGACCTTGTAGGAAGCATCA-CCCGTCCGGTAAAAGAACTGAAA 2268 0 1980 ...................................-..............................-....................... 2067 1 881 ...................................T....................N.........C..................CTG.N 970 G F D K I F L K A G E S K T V S F K I T P E L L R F Y D Query_1 2269 GGGTTTGATAAAATTTTCCT-CAAAGCGGG-TGAAAGTAAGACTGTATCTTTCAAAATCAC-TCCGGAATTACTGCGCTT-CTACGACTA 2354 0 2068 ....................-.........-..............................-..................-......... 2153 1 971 N.......AT..........N.........NN....N................-.......N..................C......... 1059 Y E L N Y V A E P G D F D I M I G G N S Q S V K T T H L S Query_1 2355 TGAACTCAACTACGTAGCC-GAACCGGGAGACTTCG-ACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACG-ACTCATCTGAGTTT 2441 0 2154 ...................-................-......................................-.............. 2240 1 1060 .NNC...G...........G..N......N......A.NT...........----.N..T...--N.........A..........-NN. 1142 L * H W K W I T D P N Query_1 2442 GTAACATTGGAAGTGGATAACGG-ATCCGAATTC 2474 0 2241 . 2241 1 1143 .........C..N..A..C....A....N..... 1176 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12140722 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,669 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115045.153 (APC115045.26-772.P(773).E525Q.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVSGEIVTGQAQNSDVAKKIEQGLVGGLLNLKGVEKIRDVQKLAIEKSRLGIPLIFGMDVVHGYETIFPIPLGLSCSWDMEAIRKSARVAAIEASADGISWTFSPMVDISRDPRWGRVSEGNGEDPFLGGAIAKAMVSGYQGIDLNNQLKRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEYMYPYQAAVDAGVGSVMASFNEIDGIPATANKWLMTDVLRKQWGFDGFVVTDFTGISEMIAHGIGDLQTVSARALNAGVDMDMVSEGFTGTIKKSIDEGKISMETLDKACRRILEAKYKLGLFDNPYKYCDLKRPKRDIFTKEHRDAARKIAGESFVLLKNDKSGSSANPTLPLKKEGTVAVIGPLANTRSNMPGTWSVAARLNDYPSVYEGLKEMMKGKVNITYAKGSNLISDAAYEERATMFGRSLNRDNRTDKEMLDEALKVAANADVIIAALGESSEMSGESSSRTNLALPDVQRTLLEALLKTGKPVVLTLFTGRPLTLTWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLLEGKWFEKFRSNYLDVDNDPLYPFGYGLSYTNFQYSDITLSAPTMGQDGSVTAMVTVTNTGKYDGAEVVQLYIRDLVGSITRPVKELKGFDKIFLKAGESKTVSFKITPELLRFYDYELNYVAEPGDFDIMIGGNSQSVKTTHLSL
AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTTTCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAAGGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCATGGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATCGAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAACGGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAACGATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAACCGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGCATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGTATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGTGAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAAGCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGCGACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAAAAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGACTATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCAGCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,552 total letters Query= APC115045.153 Length=2646 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4033 0.0 2_G2_APC115045.153Seq171G08.seq 1811 0.0 1_02G_APC115045.153Seq171G02.seq 1792 0.0 K S P Q D M D R F I D A L M K K M T V E E K I G Q L N L P V Query_1 202 AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTT 291 0 1 .......................................................................................... 90 S G E I V T G Q A Q N S D V A K K I E Q G L V G G L L N L K Query_1 292 TCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAA 381 0 91 .......................................................................................... 180 G V E K I R D V Q K L A I E K S R L G I P L I F G M D V V H Query_1 382 GGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCAT 471 0 181 .......................................................................................... 270 G Y E T I F P I P L G L S C S W D M E A I R K S A R V A A I Query_1 472 GGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATC 561 0 271 .......................................................................................... 360 E A S A D G I S W T F S P M V D I S R D P R W G R V S E G N Query_1 562 GAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAAC 651 0 361 .......................................................................................... 450 G E D P F L G G A I A K A M V S G Y Q G I D L N N Q L K R Query_1 652 GGCGAAGACCCATTCTTGGGTGGAGCCAT-CGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGA-CCTCAACAACCAACTGAAGCGCA 739 0 451 .............................-....................................-....................... 538 2 1128 ....A......N............N.-.....--........N-....A............NNG--...... 1063 N D E I M A C V K H F A L Y G A G E A G R D Y N T V D M S Query_1 740 AC-GATGAAATTATGGCATGTGTAAA-GCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTA-GATATGAGTC 826 0 539 ..-.......................-....................................................-.......... 625 2 1062 ..C......N.A...-....GN..N.N..............NNN.N..T.-........-........N..........A.......... 976 R N R M F N E Y M Y P Y Q A A V D A G V G S V M A S F N E I Query_1 827 GTAACCGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAG 916 0 626 .......................................................................................... 715 2 975 ...............-...-.................-.....-..................................-........... 891 2 949 ........... 959 D G I P A T A N K W L M T D V L R K Q W G F D G F V V T D F Query_1 917 ACGGCATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTA 1006 0 716 .......................................................................................... 805 2 890 .........................................................................-...............N 802 T G I S E M I A H G I G D L Q T V S A R A L N A G V D M D M Query_1 1007 CCGGTATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGG 1096 0 806 .......................................................................................... 895 2 801 .......................................................................................... 712 V S E G F T G T I K K S I D E G K I S M E T L D K A C R R I Query_1 1097 TAAGTGAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCC 1186 0 896 .......................................................................................... 985 2 711 .......................................................................................... 622 L E A K Y K L G L F D N P Y K Y C D L K R P K R D I F T K E Query_1 1187 TTGAAGCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAAC 1276 0 986 .......................................................................................... 1075 2 621 .......................................................................................... 532 H R D A A R K I A G E S F V L L K N D K S G S S A N P T L P Query_1 1277 ATCGCGACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTT 1366 0 1076 .......................................................................................... 1165 2 531 .......................................................................................... 442 L K K E G T V A V I G P L A N T R S N M P G T W S V A A R L Query_1 1367 TGAAAAAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCA 1456 0 1166 .......................................................................................... 1255 2 441 .......................................................................................... 352 N D Y P S V Y E G L K E M M K G K V N I T Y A K G S N L I S Query_1 1457 ACGACTATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTG 1546 0 1256 .......................................................................................... 1345 2 351 .......................................................................................... 262 D A A Y E E R A T M F G R S L N R D N R T D K E M L D E A L Query_1 1547 ATGCAGCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGA 1636 0 1346 .......................................................................................... 1435 2 261 .......................................................................................... 172 1 2 .....N......-...................... 35 K V A A N A D V I I A A L G E S S E M S G Q S S S R T N L A Query_1 1637 AAGTGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTCAGAGTTCAAGCCGCACTAACCTGGCTC 1726 0 1436 ..............................................................G.A......................... 1525 2 171 .......................................................................................... 82 1 36 .......................................................................................... 125 L P D V Q R T L L E A L L K T G K P V V L T L F T G R P L T Query_1 1727 TTCCCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGC-TCTTTACAGGTCGCCCACTAACG 1815 0 1526 ..................................................................-....................... 1614 2 81 ........................................................N.........T....N.. 8 1 126 ..................................................................-....................... 214 L T W E Q E H V P A I L N V W F G G S E A A Y A I G D V L F Query_1 1816 TTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTC 1905 0 1615 .......................................................................................... 1704 1 215 .......................................................................................... 304 G D V N P S G K L T M T F P K N V G Q I P L F Y N H K N T G Query_1 1906 GGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGT 1995 0 1705 .......................................................................................... 1794 1 305 .......................................................................................... 394 R P L L E G K W F E K F R S N Y L D V D N D P L Y P F G Y G Query_1 1996 CGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGT 2085 0 1795 .......................................................................................... 1884 1 395 .......................................................................................... 484 L S Y T N F Q Y S D I T L S A P T M G Q D G S V T A M V T V Query_1 2086 TTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTA 2175 0 1885 .......................................................................................... 1974 1 485 .......................................................................................... 574 T N T G K Y D G A E V V Q L Y I R D L V G S I T R P V K E L Query_1 2176 ACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTG 2265 0 1975 .......................................................................................... 2064 1 575 .......................................................................................... 664 K G F D K I F L K A G E S K T V S F K I T P E L L R F Y D Y Query_1 2266 AAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTAT 2355 0 2065 .......................................................................................... 2154 1 665 .......................................................................................... 754 E L N Y V A E P G D F D I M I G G N S Q S V K T T H L S L * Query_1 2356 GAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTGTAA 2445 0 2155 ....................................................................................... 2241 1 755 ......G...................................................-............................... 843 H W K W I T D P N S S S V D K L A A A L E H H H H H H * D Query_1 2446 CATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGC-TTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGAT-C 2533 1 844 ..............................................T......................................N..C. 933 P A A N K A R K E A E L A A A T A E Q * L A * P L G A S K Query_1 2534 CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGC-TGCTGCCACCGCTGAGCAATAACTAGCA-TAACC-CCTTGGGGCCTCTAAAC 2620 1 934 ........-.N..........................T...........N................N.....N....N.NNN........ 1022 R V L R G F L L K Query_1 2621 GGGTCTTGAGGGGTTTTTTGCTGAAA 2646 1 1023 ............T............. 1048 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11833129 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,552 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115045.154 (APC115045.26-772.P(773).F293W.pMCSG68)
KSPQDMDRFIDALMKKMTVEEKIGQLNLPVSGEIVTGQAQNSDVAKKIEQGLVGGLLNLKGVEKIRDVQKLAIEKSRLGIPLIFGMDVVHGYETIFPIPLGLSCSWDMEAIRKSARVAAIEASADGISWTFSPMVDISRDPRWGRVSEGNGEDPFLGGAIAKAMVSGYQGIDLNNQLKRNDEIMACVKHFALYGAGEAGRDYNTVDMSRNRMFNEYMYPYQAAVDAGVGSVMASFNEIDGIPATANKWLMTDVLRKQWGFDGFVVTDFTGISEMIAHGIGDLQTVSARALNAGVDMDMVSEGFTGTIKKSIDEGKISMETLDKACRRILEAKYKLGLFDNPYKYCDLKRPKRDIFTKEHRDAARKIAGESFVLLKNDKSGSSANPTLPLKKEGTVAVIGPLANTRSNMPGTWSVAARLNDYPSVYEGLKEMMKGKVNITYAKGSNLISDAAYEERATMFGRSLNRDNRTDKEMLDEALKVAANADVIIAALGESSEMSGESSSRTNLALPDVQRTLLEALLKTGKPVVLTLFTGRPLTLTWEQEHVPAILNVWFGGSEAAYAIGDVLFGDVNPSGKLTMTFPKNVGQIPLFYNHKNTGRPLLEGKWFEKFRSNYLDVDNDPLYPFGYGLSYTNFQYSDITLSAPTMGQDGSVTAMVTVTNTGKYDGAEVVQLYIRDLVGSITRPVKELKGFDKIFLKAGESKTVSFKITPELLRFYDYELNYVAEPGDFDIMIGGNSQSVKTTHLSL
AAGTCTCCGCAGGACATGGATCGCTTCATCGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAGATCGGTCAGCTGAACCTACCCGTTTCCGGCGAGATCGTCACCGGGCAGGCACAAAACAGCGATGTGGCAAAAAAGATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAAGGGGTGGAGAAGATACGCGATGTACAAAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCATGGTTACGAAACCATTTTCCCTATTCCATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATCGAGGCCAGTGCTGATGGTATTTCCTGGACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAACGGCGAAGACCCATTCTTGGGTGGAGCCATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAACGATGAAATTATGGCATGTGTAAAGCACTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAACCGTATGTTCAACGAATACATGTATCCCTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGCATACCAGCCACGGCCAATAAATGGCTGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTTTACCGGTATCTCCGAAATGATAGCGCACGGCATCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGTGAAGGCTTCACGGGTACAATCAAGAAATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAAGCCAAATACAAACTGGGATTATTCGACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGCGACGCTGCTCGTAAGATTGCGGGAGAGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAAAAAGAAGGTACGGTGGCTGTCATCGGCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGACTATCCTTCTGTGTACGAAGGATTGAAAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCAGCCTACGAAGAACGTGCCACAATGTTCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTGGCCGCTAATGCAGATGTAATAATAGCCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCCGATGTACAGCGCACTCTATTGGAAGCTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACTTGGGAACAGGAGCATGTGCCCGCCATCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGATGTAAATCCCAGCGGCAAACTAACGATGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCTTTACTTGAAGGCAAATGGTTCGAAAAATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCGTATACCAACTTTCAATACAGCGACATAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCAGAAGTAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGGGTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGAGACTTCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,163 total letters Query= APC115045.154 Length=2646 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4033 0.0 2_H2_APC115045.154Seq171H08.seq 1617 0.0 1_02H_H2_APC115045.154_T7S.seq 1591 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 56 AATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCT-GGTGTAGATCTG 144 2 1004 .....NNA....A..G.A..G..GA..TN. 975 1 4 ...............-.................................................T.A.........-............ 91 W S H P Q F E K G T E N L Y F Q S N A K S P Q D M D R F Query_1 145 TGGTCTCAT-CCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTT-CCAAT-CCAATGCC-AAGTCTCCGCAGGACATGGATCGCTTCAT 230 0 1 ............................. 29 2 974 ....T..N.T........N.......N...........N......N.....N.....N..C....N..N..............N...... 885 1 92 .........-...................................-.....-........-............................. 177 I D A L M K K M T V E E K I G Q L N L P V S G E I V T G Q A Query_1 231 CGATGCATTGATGAAGAAGATGACTGTAGAGGAAAAG--ATCGGTCAGCTGAACCTACCCGTTTCCGGCGAGATCGTCACCGGGCAGGCA 318 0 30 .....................................--................................................... 117 2 884 ...........AN.......................AGA.................N................................. 795 1 178 .....................................--................................................... 265 Q N S D V A K K I E Q G L V G G L L N L K G V E K I R D V Query_1 319 CAAAACAGCGATGTGGCAAAAAA-GATTGAACAAGGGCTCGTGGGCGGACTCCTCAACCTGAAAGGGGTGGAGAAGATACGCGATGTACA 407 0 118 .......................-.................................................................. 206 2 794 .......................A.................................................................. 705 1 266 .......................-.................................................................. 354 Q K L A I E K S R L G I P L I F G M D V V H G Y E T I F P I Query_1 408 AAAACTGGCCATAGAGAAGTCACGCCTGGGCATCCCCCTGATATTCGGCATGGACGTAGTGCATGGTTACGAAACCATTTTCCCTATTCC 497 0 207 .......................................................................................... 296 2 704 .......................................................................................... 615 1 355 .......................................................................................... 444 P L G L S C S W D M E A I R K S A R V A A I E A S A D G I S Query_1 498 ATTAGGCCTCTCCTGTTCCTGGGATATGGAAGCCATCAGGAAATCTGCCCGCGTTGCAGCCATCGAGGCCAGTGCTGATGGTATTTCCTG 587 0 297 .......................................................................................... 386 2 614 .......................................................................................... 525 1 445 .......................................................................................... 534 W T F S P M V D I S R D P R W G R V S E G N G E D P F L G G Query_1 588 GACATTCAGCCCGATGGTAGACATCAGCCGTGATCCGCGCTGGGGACGCGTCAGCGAGGGTAACGGCGAAGACCCATTCTTGGGTGGAGC 677 0 387 .......................................................................................... 476 2 524 .......................................................................................... 435 1 535 ..........................N............................................................... 624 A I A K A M V S G Y Q G I D L N N Q L K R N D E I M A C V K Query_1 678 CATCGCTAAAGCAATGGTATCGGGTTATCAGGGTATCGACCTCAACAACCAACTGAAGCGCAACGATGAAATTATGGCATGTGTAAAGCA 767 0 477 .......................................................................................... 566 2 434 .......................................................................................... 345 1 625 .......................................................................................... 714 H F A L Y G A G E A G R D Y N T V D M S R N R M F N E Y M Y Query_1 768 CTTCGCACTGTATGGTGCCGGAGAAGCCGGACGTGATTACAATACCGTAGATATGAGTCGTAACCGTATGTTCAACGAATACATGTATCC 857 0 567 .......................................................................................... 656 2 344 .......................................................................................... 255 1 715 .......................................................................................... 804 P Y Q A A V D A G V G S V M A S F N E I D G I P A T A N K W Query_1 858 CTACCAAGCTGCCGTAGATGCAGGTGTAGGCAGCGTAATGGCGTCTTTCAACGAAATAGACGGCATACCAGCCACGG-CCAATAAATGGC 946 0 657 .............................................................................-............ 745 2 254 .............................................................................-............ 166 1 805 ..........................................................N...........N......C...T...T.... 894 L M T D V L R K Q W G F D G F V V T D W T G I S E M I A H G Query_1 947 TGATGACCGACGTACTGCGCAAGCAATGGGGCTTCGACGGCTTTGTGGTGACAGACTGGACCGGTATCTCCGAAATGATAGCGCACGGCA 1036 0 746 .........................................................TT............................... 835 2 165 .......................................................................................... 76 1 895 ..........NN......N.. 915 I G D L Q T V S A R A L N A G V D M D M V S E G F T G T I K Query_1 1037 TCGGTGACTTGCAGACTGTTTCCGCACGTGCACTCAATGCAGGCGTGGATATGGACATGGTAAGTGAAGGCTTCACGGGTACAATCAAGA 1126 0 836 .......................................................................................... 925 2 75 ...................................................-N....-N.....-.... 10 K S I D E G K I S M E T L D K A C R R I L E A K Y K L G L F Query_1 1127 AATCCATAGACGAAGGCAAGATCAGTATGGAAACCCTGGACAAAGCCTGTCGCCGCATCCTTGAAGCCAAATACAAACTGGGATTATTCG 1216 0 926 .......................................................................................... 1015 D N P Y K Y C D L K R P K R D I F T K E H R D A A R K I A G Query_1 1217 ACAATCCTTATAAGTACTGCGACCTGAAACGCCCGAAGCGTGACATCTTCACCAAGGAACATCGCGACGCTGCTCGTAAGATTGCGGGAG 1306 0 1016 .......................................................................................... 1105 E S F V L L K N D K S G S S A N P T L P L K K E G T V A V I Query_1 1307 AGAGCTTTGTACTCCTGAAAAACGACAAGTCAGGTTCCTCTGCAAACCCAACACTTCCTTTGAAAAAAGAAGGTACGGTGGCTGTCATCG 1396 0 1106 .......................................................................................... 1195 G P L A N T R S N M P G T W S V A A R L N D Y P S V Y E G L Query_1 1397 GCCCACTGGCAAATACCCGCAGTAACATGCCGGGTACCTGGAGTGTAGCCGCACGCCTCAACGACTATCCTTCTGTGTACGAAGGATTGA 1486 0 1196 .......................................................................................... 1285 K E M M K G K V N I T Y A K G S N L I S D A A Y E E R A T M Query_1 1487 AAGAGATGATGAAAGGCAAGGTAAACATCACTTATGCCAAAGGTAGTAACCTCATCAGTGATGCAGCCTACGAAGAACGTGCCACAATGT 1576 0 1286 .......................................................................................... 1375 F G R S L N R D N R T D K E M L D E A L K V A A N A D V I I Query_1 1577 TCGGCCGTTCATTAAATCGTGATAATCGTACAGACAAAGAGATGCTGGATGAGGCGCTGAAAGTGGCCGCTAATGCAGATGTAATAATAG 1666 0 1376 .......................................................................................... 1465 A A L G E S S E M S G E S S S R T N L A L P D V Q R T L L E Query_1 1667 CCGCATTGGGAGAATCATCTGAAATGAGTGGTGAAAGTTCAAGCCGCACTAACCTGGCTCTTCCCGATGTACAGCGCACTCTATTGGAAG 1756 0 1466 .......................................................................................... 1555 A L L K T G K P V V L T L F T G R P L T L T W E Q E H V P A Query_1 1757 CTTTGCTGAAAACTGGAAAGCCTGTTGTACTGACGCTCTTTACAGGTCGCCCACTAACGTTGACTTGGGAACAGGAGCATGTGCCCGCCA 1846 0 1556 .......................................................................................... 1645 I L N V W F G G S E A A Y A I G D V L F G D V N P S G K L T Query_1 1847 TCCTGAATGTATGGTTCGGTGGAAGTGAGGCAGCATACGCCATTGGCGATGTATTGTTCGGCGATGTAAATCCCAGCGGCAAACTAACGA 1936 0 1646 .......................................................................................... 1735 M T F P K N V G Q I P L F Y N H K N T G R P L L E G K W F E Query_1 1937 TGACATTCCCCAAAAACGTAGGCCAAATACCTTTGTTTTACAATCATAAAAATACCGGTCGTCCTTTACTTGAAGGCAAATGGTTCGAAA 2026 0 1736 .......................................................................................... 1825 K F R S N Y L D V D N D P L Y P F G Y G L S Y T N F Q Y S D Query_1 2027 AATTCCGTAGTAATTACCTGGATGTAGACAACGATCCATTGTATCCATTCGGCTATGGTTTGTCGTATACCAACTTTCAATACAGCGACA 2116 0 1826 .......................................................................................... 1915 I T L S A P T M G Q D G S V T A M V T V T N T G K Y D G A E Query_1 2117 TAACTCTGAGCGCCCCGACTATGGGACAGGATGGTTCTGTTACTGCTATGGTCACGGTAACCAATACCGGTAAGTACGATGGTGCAGAAG 2206 0 1916 .......................................................................................... 2005 V V Q L Y I R D L V G S I T R P V K E L K G F D K I F L K A Query_1 2207 TAGTGCAACTTTATATCCGTGACCTTGTAGGAAGCATCACCCGTCCGGTAAAAGAACTGAAAGGGTTTGATAAAATTTTCCTCAAAGCGG 2296 0 2006 .......................................................................................... 2095 G E S K T V S F K I T P E L L R F Y D Y E L N Y V A E P G D Query_1 2297 GTGAAAGTAAGACTGTATCTTTCAAAATCACTCCGGAATTACTGCGCTTCTACGACTATGAACTCAACTACGTAGCCGAACCGGGAGACT 2386 0 2096 .......................................................................................... 2185 F D I M I G G N S Q S V K T T H L S L Query_1 2387 TCGACATAATGATCGGGGGGAACAGCCAAAGTGTAAAAACGACTCATCTGAGTTTG 2442 0 2186 ........................................................ 2241 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 10810448 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,163 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115077.128 (APC115077.27-740.P(800).Y480A.pMCSG68)
REQSFDEAWLFHRGDIAEGEKQSLDDSQWRQINLPHDWSIEDIPGTNSPFTADAATEVAGGFTVGGTGWYRKHFYIDAAEKGKCIAVSFDGIYMNADIWVNDRHVANHVYGYTAFELDITDYVRFGAENLIAVRVKNEGMNCRWYTGSGIYRHTFLKITNPLHFETWGTFVTTPVATADKAEVHVQSVLANTEKVTGKVILETRIVDKNNHTVARKEQLVTLDNKEKTEVGHALEVLAPQLWSIDNPYLYQVVNRLLQDDKVIDEEYISIGIRNIAFSAENGFQLNGKSMKLKGGCIHHDNGLLGAKAFDRAEERKIELLKAAGFNALRLSHNPPSIALLNACDRLGMLVIDEAFDMWRYGHYQYDYAQYFDKLWKEDLHSMVARDRNHPSVIMWSIGNEIKNKETAEIVDICRELTGFVKTLDTTRPVTAGVNSIVDATDDFLAPLDVCGYNYCLNRYESDAKRHPDRIIYASESYASQAYDYWKGVEDHSWVIGDFIWTAFDYIGEASIGWCGYPLDKRIFPWNHANCGDLNLSGERRPQSYLRETLWSDAPVSHIVVTPPVPSFPLNPDKADWSVWDFPDVVDHWNFPGYEGKKMTVSVYSNCEQVELFLNGESLGKQENTADKKNTLVWEVPYAHGILKAVSYNKGGEVGTATLESAGKVEKIRLSADRTEIVADGNDLSYITLELVDSKGIRNQLAEELVAFSIEGDAT
CGGGAACAATCTTTCGATGAGGCATGGTTATTTCATCGTGGAGATATTGCCGAAGGAGAAAAGCAATCTTTAGATGACTCACAATGGCGTCAGATAAATCTTCCTCATGATTGGAGTATTGAAGATATTCCTGGAACCAATTCTCCTTTTACAGCGGATGCTGCAACGGAAGTTGCAGGTGGTTTTACTGTAGGTGGTACGGGATGGTATAGAAAGCACTTCTACATAGATGCGGCTGAAAAAGGTAAATGTATTGCTGTCTCTTTCGATGGAATTTATATGAATGCAGATATCTGGGTGAATGATCGCCATGTAGCCAATCATGTTTATGGATATACTGCATTTGAACTGGATATAACCGATTATGTACGTTTCGGAGCTGAAAATCTGATAGCTGTCCGTGTGAAGAATGAAGGTATGAATTGCCGTTGGTATACAGGTTCGGGTATTTACAGGCATACTTTCTTGAAGATAACCAATCCGCTTCATTTTGAAACTTGGGGAACGTTTGTCACGACTCCCGTTGCAACGGCGGATAAAGCAGAGGTACATGTACAGAGTGTTCTGGCAAATACTGAAAAAGTAACCGGAAAAGTGATTCTGGAAACGCGGATTGTAGATAAGAATAACCATACTGTAGCTCGGAAAGAGCAACTGGTAACATTGGATAACAAAGAAAAAACAGAGGTTGGCCATGCGTTGGAAGTGCTTGCTCCGCAATTATGGTCTATAGACAATCCTTACTTATATCAGGTTGTAAACCGTCTTCTGCAAGATGATAAAGTTATAGATGAGGAATATATTTCAATAGGTATACGCAATATTGCATTTAGTGCGGAGAATGGTTTCCAGCTGAATGGTAAATCCATGAAACTAAAAGGCGGATGTATCCATCATGACAATGGTCTTTTGGGTGCAAAGGCTTTTGACCGGGCAGAGGAAAGGAAAATAGAACTACTGAAAGCGGCTGGTTTCAATGCGCTGCGCTTGTCTCATAATCCTCCCTCAATCGCTTTACTCAATGCCTGCGACCGCTTAGGTATGCTGGTCATAGATGAGGCTTTTGATATGTGGCGCTATGGTCATTATCAGTATGATTATGCACAATACTTTGATAAATTGTGGAAAGAAGATTTGCATAGTATGGTTGCACGGGATAGGAATCATCCTAGTGTTATCATGTGGAGTATTGGTAATGAAATCAAGAACAAAGAAACTGCTGAAATTGTGGATATATGCAGGGAGTTGACAGGTTTTGTGAAGACGCTTGATACAACGCGGCCTGTTACGGCGGGAGTTAATTCTATTGTTGATGCAACGGATGATTTTCTGGCTCCTCTGGATGTTTGTGGTTATAATTACTGTTTAAACCGTTATGAATCGGATGCCAAACGTCATCCGGACCGTATTATCTATGCTTCGGAGTCCTACGCATCCCAGGCTTATGATTATTGGAAAGGAGTAGAAGATCATTCATGGGTGATCGGTGATTTTATCTGGACTGCTTTTGACTATATTGGTGAGGCAAGTATCGGCTGGTGTGGGTATCCGCTTGATAAACGTATTTTCCCTTGGAATCATGCCAATTGTGGTGATTTGAATCTTTCGGGCGAACGTCGTCCCCAGTCCTATTTGCGTGAAACGTTATGGAGTGATGCACCGGTATCCCATATTGTTGTGACGCCTCCTGTTCCTTCTTTTCCTCTGAATCCGGATAAGGCGGATTGGAGTGTATGGGATTTTCCGGATGTTGTGGATCATTGGAATTTCCCGGGATATGAGGGGAAAAAGATGACAGTATCTGTATACTCCAATTGTGAACAGGTTGAACTGTTCTTGAATGGGGAATCTTTAGGAAAACAAGAAAATACTGCCGATAAGAAAAATACGCTTGTCTGGGAAGTACCTTATGCTCATGGAATATTGAAAGCCGTAAGTTATAATAAAGGCGGTGAAGTGGGCACTGCAACGTTGGAAAGTGCTGGTAAGGTTGAAAAGATCAGATTATCTGCGGACAGAACGGAAATCGTAGCTGATGGTAATGATCTAAGCTATATCACATTAGAATTGGTAGATAGTAAAGGCATTAGAAATCAGTTGGCTGAAGAATTGGTAGCATTTTCTATAGAAGGAGATGCTACG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,399 total letters Query= APC115077.128 Length=2547 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3849 0.0 2_A3_APC115077.128Seq171A09.seq 1826 0.0 1_03A_APC115077.128Seq171A03.seq 1712 0.0 R E Q S F D E A W L F H R G D I A E G E K Q S L D D S Q W R Query_1 202 CGGGAACAATCTTTCGATGAGGCATGGTTATTTCATCGTGGAGATATTGCCGAAGGAGAAAAGCAATCTTTAGATGACTCACAATGGCGT 291 0 1 .......................................................................................... 90 Q I N L P H D W S I E D I P G T N S P F T A D A A T E V A G Query_1 292 CAGATAAATCTTCCTCATGATTGGAGTATTGAAGATATTCCTGGAACCAATTCTCCTTTTACAGCGGATGCTGCAACGGAAGTTGCAGGT 381 0 91 .......................................................................................... 180 G F T V G G T G W Y R K H F Y I D A A E K G K C I A V S F D Query_1 382 GGTTTTACTGTAGGTGGTACGGGATGGTATAGAAAGCACTTCTACATAGATGCGGCTGAAAAAGGTAAATGTATTGCTGTCTCTTTCGAT 471 0 181 .......................................................................................... 270 G I Y M N A D I W V N D R H V A N H V Y G Y T A F E L D I T Query_1 472 GGAATTTATATGAATGCAGATATCTGGGTGAATGATCGCCATGTAGCCAATCATGTTTATGGATATACTGCATTTGAACTGGATATAACC 561 0 271 .......................................................................................... 360 D Y V R F G A E N L I A V R V K N E G M N C R W Y T G S G Query_1 562 GATTATGTACGTTTCGGAGCTGAAAATCTGA-TAGCTGTCCGTGTGAAGAATGAAGGTATGAATTGCCGTTGGTATACAGG-TTCGGGTA 649 0 361 ...............................-.................................................-........ 448 2 1128 ......T...GN...NT.....NN-...........NNG--.N....N.A.TA.N.....G.......N 1063 I Y R H T F L K I T N P L H F E T W G T F V T T P V A T A Query_1 650 TTTACAGGCA-TACTTTCTTGAAGATAACCAATCCGCTTCATTTTGAAACTTGGGGAACGTTTGTCACGACTCCCG-TTGCAACGGCGGA 737 0 449 ..........-.................................................................-............. 536 0 532 ...-......... 521 2 1062 ..........N.N..............N.-......N.......-......A...........N........G...G............. 975 D K A E V H V Q S V L A N T E K V T G K V I L E T R I V D K Query_1 738 TAAAGCAGAGGTACATGTACAGAGTGTTCTGGCAAATACTGAAAAAGTAACCGGAAAAGTGATTCTGGAAACGCGGATTGTAGATAAGAA 827 0 537 .......................................................................................... 626 2 974 ..........................G.T........T........NN....A..................................... 885 N N H T V A R K E Q L V T L D N K E K T E V G H A L E V L A Query_1 828 TAACC-ATACTGTAGCTCGGAAAGAGCAACTGGTAACATTGGATAACAAAGAAAAAACAGAGGTTGGCCATGCGTTGGAAGTGCTTGCTC 916 0 627 .....-.................................................................................... 715 2 884 .....C.................................................................................... 795 P Q L W S I D N P Y L Y Q V V N R L L Q D D K V I D E E Y I Query_1 917 CGCAATTATGGTCTATAGACAATCCTTACTTATATCAGGTTGTAAACCGTCTTCTGCAAGATGATAAAGTTATAGATGAGGAATATATTT 1006 0 716 .......................................................................................... 805 2 794 .......................................................................................... 705 S I G I R N I A F S A E N G F Q L N G K S M K L K G G C I H Query_1 1007 CAATAGGTATACGCAATATTGCATTTAGTGCGGAGAATGGTTTCCAGCTGAATGGTAAA-TCCATGAAACTAAAAGGCGGATGTATCCAT 1095 0 806 ...........................................................-.............................. 894 2 704 ...........................................................A.............................. 615 H D N G L L G A K A F D R A E E R K I E L L K A A G F N A L Query_1 1096 CATGACAATGGTCTTTTGGGTGCAAAGGCTTTTGACCGGGCAGAGGAAAGGAAAATAGAACTACTGAAAGCGGCTGGTTTCAATGCGCTG 1185 0 895 .......................................................................................... 984 2 614 .......................................................................................... 525 R L S H N P P S I A L L N A C D R L G M L V I D E A F D M W Query_1 1186 CGCTTGTCTCATAATCCTCCCTCAATCGCTTTACTCAATGCCTGCGACCGCTTAGGTATGCTGGTCATAGATGAGGCTTTTGATATGTGG 1275 0 985 .......................................................................................... 1074 2 524 .......................................................................................... 435 R Y G H Y Q Y D Y A Q Y F D K L W K E D L H S M V A R D R N Query_1 1276 CGCTATGGTCATTATCAGTATGATTATGCACAATACTTTGATAAATTGTGGAAAGAAGATTTGCATAGTATGGTTGCACGGGATAGGAAT 1365 0 1075 .......................................................................................... 1164 2 434 .......................................................................................... 345 1 5 ...N..................................................... 61 H P S V I M W S I G N E I K N K E T A E I V D I C R E L T G Query_1 1366 CATCCTAGTGTTATCATGTGGAGTATTGGTAATGAAATCAAGAACAAAGAAACTGCTGAAATTGTGGATATATGCAGGGAGTTGACAGGT 1455 0 1165 .......................................................................................... 1254 2 344 .......................................................................................... 255 1 62 .......................................................................................... 151 F V K T L D T T R P V T A G V N S I V D A T D D F L A P L D Query_1 1456 TTTGTGAAGACGCTTGATACAACGCGGCCTGTTACGGCGGGAGTTAATTCTATTGTTGATGCAACGGATGATTTTCTGGCTCCTCTGGAT 1545 0 1255 .......................................................................................... 1344 2 254 .......................................................................................... 165 1 152 .......................................................................................... 241 V C G Y N A C L N R Y E S D A K R H P D R I I Y A S E S Y A Query_1 1546 GTTTGTGGTTATAATGCTTGTTTAAACCGTTATGAATCGGATGCCAAACGTCATCCGGACCGTATTATCTATGCTTCGGAGTCCTACGCA 1635 0 1345 ...............TAC........................................................................ 1434 2 164 .......................................................................................... 75 1 242 .......................................................................................... 331 S Q A Y D Y W K G V E D H S W V I G D F I W T A F D Y I G Query_1 1636 TCC-CAGGCTTATGATTATTGGAAAGGAGTAGAAGATCATTCATGGGTGATCGGTGATTTTATCTGGACTGCTTTTGACTATATTGGTGA 1724 0 1435 ...-...................................................................................... 1523 2 74 ...A.........A....................................................... 6 1 332 ...-.........A............................................................................ 420 E A S I G W C G Y P L D K R I F P W N H A N C G D L N L S G Query_1 1725 GGCAAGTATCGGCTGGTGTGGGTATCCGCTTGATAAACGTATTTTCCCTTGGAATCATGCCAATTGTGGTGATTTGAATCTTTCGGGCGA 1814 0 1524 .......................................................................................... 1613 1 421 .......................................................................................... 510 E R R P Q S Y L R E T L W S D A P V S H I V V T P P V P S F Query_1 1815 ACGTCGTCCCCAGTCCTATTTGCGTGAAACGTTATGGAGTGATGCACCGGTATCCCATATTGTTGTGACGCCTCCTGTTCCTTCTTTTCC 1904 0 1614 .......................................................................................... 1703 1 511 .......................................................................................... 600 P L N P D K A D W S V W D F P D V V D H W N F P G Y E G K K Query_1 1905 TCTGAATCCGGATAAGGCGGATTGGAGTGTATGGGATTTTCCGGATGTTGTGGATCATTGGAATTTCCCGGGATATGAGGGGAAAAAGAT 1994 0 1704 .......................................................................................... 1793 1 601 .......................................................................................... 690 M T V S V Y S N C E Q V E L F L N G E S L G K Q E N T A D K Query_1 1995 GACAGTATCTGTATACTCCAATTGTGAACAGGTTGAACTGTTCTTGAATGGGGAATCTTTAGGAAAACAAGAAAATACTGCCGATAAGAA 2084 0 1794 .......................................................................................... 1883 1 691 ............................................................NN.........-.................. 779 K N T L V W E V P Y A H G I L K A V S Y N K G G E V G T A T Query_1 2085 AAATACGCTTGTCTGGGAAGTACCTTATGCTCATGGAATATTGAAAGCCGTAAGTTATAATAAAGGCGGTGAAGTGGGCACTGCAACGTT 2174 0 1884 .......................................................................................... 1973 1 780 .....................................................................N.................... 869 L E S A G K V E K I R L S A D R T E I V A D G N D L S Y I T Query_1 2175 GGAAAGTGCTGGTAAGGTTGAAAAGATCAG-ATTATCTGCGGACAGAACGGAAA-TCGTAGCTGATGGTAATGATCTAAGCTATATCACA 2262 0 1974 ..............................-.......................-................................... 2061 1 870 ............N.......-......N..A..N....................T..............-...................- 956 L E L V D S K G I R N Q L A E E L V A F S I E G D A T * H W Query_1 2263 TTAGAATTGGTAGATAGTAAAGGCATTAGAAATCAGTTGGCTGAAGAATTGGTAGCATTTTCTATAGAAGGAGATGCTACGTAACATTGG 2352 0 2062 ................................................................................. 2142 0 658 .......... 667 2 852 .......... 843 1 957 ....-........N.N..-..........-.....-...N.NN..-..........-C.....-.N.N..N.T.....NNC.T....... 1039 K W I T D P N S S S V D K L A A Query_1 2353 AAGTGGATAACGGA-TCCGAA-TTCGAGC-TCCGTCGACAAGCTTGCGGCC 2400 0 668 . 668 2 842 . 842 1 1040 C.N...NAN.N...T..T...T.......T..T...T.AC..N...N.... 1090 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11000440 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,399 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: APC115086.149 (APC115086.29-766.P(766).W430A.pMCSG68)
KSPVDMDRFIDDLMKKMTLEEKIGQLNLPVTGEITTGQAKSSNVAKRIRAGEVGGLFNLKGVERIRDVQKQAVEESRLGIPLLFGMDVIHGYETVFPIPLGLSCTWNMTAIEESARIAAIEASADGICWTFSPMVDVSRDPRWGRVSEGNGEDPFLGAEIARAMVRGYQGKDMSSNDEIMACVKHFALYGASEAGRDYNTVDMSHQRMFNEYMLPYQAAVEEGVGSVMASFNEVDGVPATGNKWLMTDVLRKQWNFDGFVVTDYTGITEMTDHGMGDTQTVAALALNAGVDMDMVSDAFTSTLKKSLEEGKVSVKAVDAACRRILEAKYKLGLFDNPYKYCDITRPKKQIFTKEHRAIARKTASESFVLLKNENSVLPLAKKGTIAVVGPLADSRSNMPGTWSVAAVMNKYPSLIEGLKEVVGGKAKILTAKGSNLMSDAEYEERATMFGRTLHRDNRTDKELLDEALAVAAKSDVIVAALGESSEMSGESSCRTDLEMPDTQRVLLQELLKTGKPVVLVLFTGRPLVLNWEQENVPAILNVWFGGSEAALAIGDVLFGNVNPSGKLTTTFPKSVGQIPLFYNHKNTGRPLPQGAWFQKFRSNYLDVDNEPLYPFGYGLSYTTFSYSDITLDKSSMNINGEIMATVTVTNTGKYDGSEVVQLYIRDLIGSVTRPVKELKGFEKIFLKAGESKQVSFKLTADMLKFYNYNLDFVCEPGDFEVMIGGDSRDVNKALFSLQ
AAATCTCCTGTCGATATGGATCGCTTTATTGATGATCTGATGAAGAAGATGACTCTGGAAGAGAAAATCGGCCAGTTGAACTTGCCTGTTACGGGTGAAATAACCACCGGACAAGCCAAGAGTAGTAATGTGGCTAAGCGTATCCGTGCCGGTGAAGTGGGCGGACTCTTTAACTTGAAAGGCGTGGAGCGTATTCGTGACGTTCAGAAACAGGCAGTAGAAGAAAGTCGTCTGGGTATTCCTCTTTTATTTGGTATGGATGTAATTCATGGATACGAAACGGTATTTCCTATTCCTCTGGGATTATCCTGTACCTGGAACATGACAGCTATTGAAGAATCTGCACGTATTGCTGCTATCGAAGCCAGTGCTGATGGTATTTGCTGGACATTCAGTCCGATGGTGGATGTTTCCCGTGATCCCCGTTGGGGACGAGTTTCCGAAGGGAATGGTGAAGATCCCTTCTTGGGAGCGGAGATTGCGCGTGCTATGGTACGTGGTTATCAAGGGAAAGATATGAGTAGTAATGATGAAATTATGGCTTGCGTGAAGCACTTTGCGTTATATGGGGCATCAGAAGCCGGACGCGACTATAATACAGTGGATATGAGTCATCAACGTATGTTCAACGAATATATGTTACCTTATCAGGCTGCCGTGGAAGAAGGTGTGGGTAGTGTGATGGCTTCATTCAATGAAGTGGATGGTGTACCGGCTACCGGAAATAAGTGGCTGATGACCGATGTACTTCGTAAGCAGTGGAATTTTGATGGGTTCGTTGTGACGGACTATACCGGTATCACTGAAATGACCGATCATGGTATGGGTGATACACAAACAGTTGCAGCCCTGGCTCTGAATGCAGGTGTCGATATGGATATGGTGAGCGATGCTTTTACAAGCACACTTAAAAAATCTCTGGAAGAAGGAAAAGTTTCAGTAAAGGCTGTTGATGCTGCTTGTCGCCGTATTCTGGAAGCTAAGTATAAGCTGGGGCTTTTTGATAATCCCTATAAATATTGTGATATAACCCGTCCTAAAAAACAAATCTTTACAAAAGAACACCGCGCTATAGCCCGTAAGACAGCTTCGGAAAGCTTTGTTCTCTTGAAGAATGAGAATAGTGTACTCCCTCTGGCAAAGAAAGGTACCATTGCTGTAGTAGGTCCTTTGGCCGATAGCCGTAGCAATATGCCGGGCACGTGGAGTGTGGCCGCTGTGATGAACAAATATCCTTCTTTGATTGAAGGCTTGAAAGAAGTAGTGGGAGGCAAGGCTAAAATTCTTACGGCTAAAGGAAGTAATCTGATGAGTGATGCCGAATACGAAGAACGTGCTACTATGTTTGGCCGTACTCTGCATCGTGACAATCGTACAGATAAGGAACTGCTGGATGAGGCGCTTGCTGTAGCTGCCAAGTCTGACGTGATTGTTGCTGCTTTGGGTGAGTCTTCCGAGATGAGCGGTGAAAGTAGTTGCCGTACAGACCTCGAAATGCCGGATACGCAACGTGTACTTTTGCAGGAATTGTTGAAAACCGGCAAACCGGTGGTATTGGTGTTGTTTACCGGTCGTCCGTTAGTATTGAATTGGGAGCAGGAAAATGTACCTGCTATTCTGAATGTGTGGTTTGGTGGTAGTGAAGCTGCTCTTGCCATTGGTGATGTACTGTTTGGAAATGTAAATCCGAGTGGCAAACTTACTACTACTTTTCCGAAGAGTGTAGGACAGATTCCTTTGTTCTATAACCATAAGAATACTGGTCGTCCTTTGCCTCAAGGGGCCTGGTTCCAGAAGTTCCGTAGCAATTATCTGGATGTAGATAACGAACCGCTTTATCCGTTTGGATATGGCTTGAGCTATACTACTTTCTCTTATAGTGATATTACATTGGATAAATCGTCCATGAATATCAATGGAGAGATTATGGCAACTGTAACGGTAACCAATACAGGTAAGTATGACGGTTCGGAAGTAGTGCAGCTATATATCCGCGATCTTATAGGCAGTGTAACACGTCCGGTGAAAGAACTGAAAGGCTTTGAAAAAATCTTCTTGAAAGCCGGTGAATCCAAACAAGTGTCTTTCAAGTTAACAGCTGATATGTTGAAGTTCTACAATTACAATCTGGATTTTGTGTGCGAACCGGGTGACTTTGAAGTAATGATAGGTGGTGATAGCCGTGATGTGAATAAGGCCTTATTTTCGCTTCAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,457 total letters Query= APC115086.149 Length=2619 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3979 0.0 2_B3_APC115086.149Seq171B09.seq 2021 0.0 1_03B_APC115086.149Seq171B03.seq 1746 0.0 K S P V D M D R F I D D L M K K M T L E E K I G Q L N L P V Query_1 202 AAATCTCCTGTCGATATGGATCGCTTTATTGATGATCTGATGAAGAAGATGACTCTGGAAGAGAAAATCGGCCAGTTGAACTTGCCTGTT 291 0 1 .......................................................................................... 90 0 867 ............. 879 2 704 ............. 692 T G E I T T G Q A K S S N V A K R I R A G E V G G L F N L K Query_1 292 ACGGGTGAAATAACCACCGGACAAGCCAAGAGTAGTAATGTGGCTAAGCGTATCCGTGCCGGTGAAGTGGGCGGACTCTTTAACTTGAAA 381 0 91 .......................................................................................... 180 0 2095 ........... 2085 0 519 ........... 529 2 1051 ........... 1041 G V E R I R D V Q K Q A V E E S R L G I P L L F G M D V I H Query_1 382 GGCGTGGAGCGTATTCGTGACGTTCAGAAACAGGCAGTAGAAGAAAGTCGTCTGGGTATTCCTCTTTTATTTGGTATGGATGTAATTCAT 471 0 181 .......................................................................................... 270 0 2084 . 2084 G Y E T V F P I P L G L S C T W N M T A I E E S A R I A A I Query_1 472 GGATACGAAACGGTATTTCCTATTCCTCTGGGATTATCCTGTACCTGGAACATGACAGCTATTGAAGAATCTGCACGTATTGCTGCTATC 561 0 271 .......................................................................................... 360 E A S A D G I C W T F S P M V D V S R D P R W G R V S E G Query_1 562 GAAGCCAGTGCTGATGGTATTTGCTGGACATTCAGTCCGATGGTGGATGTTTCCCGTGAT-CCCCGTTGGGGACGAGTTTCCGAAGGGAA 650 0 361 ............................................................-............................. 449 2 1172 ............N....T.......T..............NNN.-.......-.. 1120 N G E D P F L G A E I A R A M V R G Y Q G K D M S S N D E I Query_1 651 TGGTGAAGATCCCTTCTTGGGAGCGGAGATTGCGCGTGCTATGGTACGTGGTTATCAAGGGAAAGATATGAGTAGTAATGATGAAATTAT 740 0 450 .......................................................................................... 539 0 120 ........... 130 2 1119 ...........................AGA......................-.....N............................... 1031 M A C V K H F A L Y G A S E A G R D Y N T V D M S H Q R M F Query_1 741 GGCTTGCGTGAAGCACTTTGCGTTATATGGGGCATCAGAAGCCGGACGCGACTATAATACAGTGGATATGAGTCATCAACGTATGTTCAA 830 0 540 .......................................................................................... 629 2 1030 ................N.-....................................................................... 942 N E Y M L P Y Q A A V E E G V G S V M A S F N E V D G V P A Query_1 831 CGAATATATGTTACCTTATCAGGCTGCCGTGGAAGAAGGTGTGGGTAGTGTGATGGCTTCATTCAATGAAGTGGATGGTGTACCGGCTAC 920 0 630 .......................................................................................... 719 2 941 .......................................................................................... 852 T G N K W L M T D V L R K Q W N F D G F V V T D Y T G I T E Query_1 921 CGGAAATAAGTGGCTGATGACCGATGTACTTCGTAAGCAGTGGAATTTTGATGGGTTCGTTGTGACGGACTATACCGGTATCACTGAAAT 1010 0 720 .......................................................................................... 809 2 851 .......................................................................................... 762 M T D H G M G D T Q T V A A L A L N A G V D M D M V S D A F Query_1 1011 GACCGATCATGGTATGGGTGATACACAAACAGTTGCAGCCCTGGCTCTGAATGCAGGTGTCGATATGGATATGGTGAGCGATGCTTTTAC 1100 0 810 .......................................................................................... 899 0 9 ............. 21 2 761 .......................................................................................... 672 T S T L K K S L E E G K V S V K A V D A A C R R I L E A K Y Query_1 1101 AAGCACACTTAAAAAATCTCTGGAAGAAGGAAAAGTTTCAGTAAAGGCTGTTGATGCTGCTTGTCGCCGTATTCTGGAAGCTAAGTATAA 1190 0 900 .......................................................................................... 989 2 671 .......................................................................................... 582 K L G L F D N P Y K Y C D I T R P K K Q I F T K E H R A I A Query_1 1191 GCTGGGGCTTTTTGATAATCCCTATAAATATTGTGATATAACCCGTCCTAAAAAACAAATCTTTACAAAAGAACACCGCGCTATAGCCCG 1280 0 990 .......................................................................................... 1079 2 581 .......................................................................................... 492 R K T A S E S F V L L K N E N S V L P L A K K G T I A V V G Query_1 1281 TAAGACAGCTTCGGAAAGCTTTGTTCTCTTGAAGAATGAGAATAGTGTACTCCCTCTGGCAAAGAAAGGTACCATTGCTGTAGTAGGTCC 1370 0 1080 .......................................................................................... 1169 2 491 .......................................................................................... 402 1 6 ................-........................... 48 P L A D S R S N M P G T A S V A A V M N K Y P S L I E G L K Query_1 1371 TTTGGCCGATAGCCGTAGCAATATGCCGGGCACGGCTAGTGTGGCCGCTGTGATGAACAAATATCCTTCTTTGATTGAAGGCTTGAAAGA 1460 0 1170 ..................................TGG..................................................... 1259 2 401 .......................................................................................... 312 1 49 .......................................................................................... 138 E V V G G K A K I L T A K G S N L M S D A E Y E E R A T M F Query_1 1461 AGTAGTGGGAGGCAAGGCTAAAATTCTTACGGCTAAAGGAAGTAATCTGATGAGTGATGCCGAATACGAAGAACGTGCTACTATGTTTGG 1550 0 1260 .......................................................................................... 1349 2 311 .......................................................................................... 222 1 139 .......................................................................................... 228 G R T L H R D N R T D K E L L D E A L A V A A K S D V I V A Query_1 1551 CCGTACTCTGCATCGTGACAATCGTACAGATAAGGAACTGCTGGATGAGGCGCTTGCTGTAGCTGCCAAGTCTGACGTGATTGTTGCTGC 1640 0 1350 .......................................................................................... 1439 2 221 .......................................................................................... 132 1 229 .......................................................................................... 318 A L G E S S E M S G E S S C R T D L E M P D T Q R V L L Q E Query_1 1641 TTTGGGTGAGTCTTCCGAGATGAGCGGTGAAAGTAGTTGCCGTACAGACCTCGAAATGCCGGATACGCAACGTGTACTTTTGCAGGAATT 1730 0 1440 .......................................................................................... 1529 2 131 .......................................................................................... 42 1 319 .......................................................................................... 408 L L K T G K P V V L V L F T G R P L V L N W E Q E N V P A I Query_1 1731 GTTGAAAACCGGCAAACCGGTGGTATTGGTGTTGTTTACCGGTCGTCCGTTAGTATTGAATTGGGAGCAGGAAAATGTACCTGCTATTCT 1820 0 1530 .......................................................................................... 1619 2 41 .............................. 12 1 409 .......................................................................................... 498 L N V W F G G S E A A L A I G D V L F G N V N P S G K L T T Query_1 1821 GAATGTGTGGTTTGGTGGTAGTGAAGCTGCTCTTGCCATTGGTGATGTACTGTTTGGAAATGTAAATCCGAGTGGCAAACTTACTACTAC 1910 0 1620 .......................................................................................... 1709 1 499 .......................................................................................... 588 T F P K S V G Q I P L F Y N H K N T G R P L P Q G A W F Q K Query_1 1911 TTTTCCGAAGAGTGTAGGACAGATTCCTTTGTTCTATAACCATAAGAATACTGGTCGTCCTTTGCCTCAAGGGGCCTGGTTCCAGAAGTT 2000 0 1710 .......................................................................................... 1799 1 589 .......................................................................................... 678 F R S N Y L D V D N E P L Y P F G Y G L S Y T T F S Y S D I Query_1 2001 CCGTAGCAATTATCTGGATGTAGATAACGAACCGCTTTATCCGTTTGGATATGGCTTGAGCTATACTACTTTCTCTTATAGTGATATTAC 2090 0 1800 .......................................................................................... 1889 1 679 .......................................................................................... 768 T L D K S S M N I N G E I M A T V T V T N T G K Y D G S E V Query_1 2091 ATTGGATAAATCGTCCATGAATATCAATGGAGAGATTATGGCAACTGTAACGGTAA-CCAATACAGGTAAGTATGACGGTTCGGAAGTAG 2179 0 1890 ........................................................-................................. 1978 1 769 ........................................................C................................. 858 V Q L Y I R D L I G S V T R P V K E L K G F E K I F L K A Query_1 2180 TGCAGCTATATATCCGCGA-TCTTATAGGCAGTGTAACACGTCCGGTGAAAGAA-CTGAAAGGCTTTGAAAAAATCTTCTTGAAAGCCGG 2267 0 1979 ...................-..................................-................................... 2066 1 859 ...................C..................................N...-.............................T. 947 G E S K Q V S F K L T A D M L K F Y N Y N L D F V C E P G Query_1 2268 TGAATCCAAACAAGTGTC-TTTCAAGTTAACAGCTG-ATATGTTGAAGTTCTACAATTACAA-TCTGGATTTTGTGTGCGAACCGGGTGA 2354 0 2067 ..................-.................-.........................-........................... 2153 0 181 .-........... 170 1 948 .......-..........T.........-.......A......GN..N....-...-.....T..N.........N...........-.N 1032 D F E V M I G G D S R D V N K A L F S L Q Query_1 2355 CTTTGAAGTAATGATAGGTGGTG--ATAGCCGTGATGTGAATAAGGCCTTATTTTCGCTTCAA 2415 0 2154 .......................--...................................... 2214 0 2204 .............. 2191 1 1033 .-..N.....T...N.C..T...GN..NN..... 1065 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11464412 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,457 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200018.120 (CMR200018.20-949.P(949).E413Q.pMCSG68)
QTLPQTERQYLSGHGCDDTVEWDFFCTDGRNSGRWTKIGVPSCWELQGFGTYQYGISFYGKAFPEGIAGEKGMYKYEFEVPEEFRGKQVSLVFEASMTDTEVKVNGRKAGSKHQGAFYCFSYNVTDLLKYGKKNQLEVTVSKESENASVNLAERRADYWNFGGIFRPVFLEVKPAVNLRHIAIDAQMDGSFRANCYTNISGDGMSIRAQILDGKGKKLADTTVPLKAGSDWTTLQLNVSAPALWTAETPNLYKAQFSLLDKGGKVLHHETETFGFRTIEVRESDGLYVNGVRINVRGVNRHSFRPESGRTLSKAKNIEDVLLMKGMNMNSVRLSHYPADPEFLEACDSLGLYVMDELGGWHGKYDTPTGVRLIEGMIERDVNHPSIIWWSNGNEKGWNIELDGEFHKYDLQKRPVIHPQGNFSGFETMHYRSYGESQNYMRLPEIFMPTEFLHGLYDGGHGAGLYDYWEMMRKHPRCIGGFLWVLADEGVKRVDMDGFIDNQGNFGADGIVGPHHEKEGSYYTIKQLWSPVQVMNTAIDRNFDGKLSVENRYDYLNLNTCRFIWQQVKFPSVTDASNTTTRILKQGEVQGSDVAAHGVGVVDIKTSILPEADALFLTVIDKYGYELWRWTFPVDKLNRETEQFSASSGRVSYTETEKGITVKANGRTFVFSKKDGQLKDVSVNNRKISFANGPRFIGARRADRSLDQFYNHDDEKAKAKDRTYSEFTDAAVFTKLDVKEEGGNLILTANYKLGNLDKAQWTIHPDGMATLDYTYNFSGVVDLMGICFDYPEEQVLSKRWLGAGPYRVWQNRIHGTQYDIWENDYNDPIPGETFTYPEFKGYFGSVSWMSIRTKEGTISLTNETPDSYIGVYQPRDGRDRLLYTLPESGISVLNVIPPVRNKVNSTDLCGPSSQPKWVDGSQTGRLVIRFE
CAGACACTTCCGCAGACAGAGCGGCAATACCTCTCCGGCCACGGATGCGACGACACAGTAGAATGGGACTTTTTCTGTACCGACGGACGTAACTCCGGTCGATGGACGAAAATAGGCGTCCCCTCTTGCTGGGAGTTGCAGGGTTTTGGTACCTATCAGTATGGAATTAGTTTTTATGGTAAAGCCTTTCCCGAAGGCATTGCCGGTGAGAAAGGAATGTATAAATATGAGTTTGAAGTTCCCGAGGAATTTCGTGGCAAGCAGGTCAGCCTTGTGTTCGAAGCATCCATGACCGATACGGAAGTTAAGGTTAACGGACGTAAGGCAGGATCGAAACACCAGGGAGCCTTCTATTGCTTTTCATATAATGTCACGGATTTACTGAAATATGGCAAGAAGAATCAGCTGGAAGTAACAGTTTCCAAGGAGAGTGAGAATGCCAGTGTGAATCTTGCCGAACGGCGCGCCGATTATTGGAACTTTGGCGGTATCTTCCGCCCGGTATTTCTGGAAGTAAAACCTGCCGTCAATCTCCGTCATATTGCTATTGATGCACAAATGGACGGATCATTCCGTGCCAATTGCTACACGAATATCTCCGGTGACGGAATGAGTATCCGTGCACAGATTTTGGACGGTAAAGGGAAGAAACTGGCAGATACCACCGTACCCCTAAAAGCCGGAAGCGACTGGACTACTTTACAATTGAACGTTTCTGCCCCTGCCTTATGGACGGCAGAAACTCCGAATCTTTATAAAGCTCAATTTTCACTGTTGGATAAAGGAGGTAAAGTCCTGCATCATGAGACCGAGACATTCGGTTTCCGTACTATCGAAGTTCGTGAAAGTGACGGATTGTACGTGAACGGGGTGCGTATCAACGTGCGTGGTGTCAACCGTCATAGTTTCCGTCCCGAAAGCGGTCGTACCCTAAGTAAAGCGAAGAATATTGAAGATGTACTTCTGATGAAGGGCATGAATATGAATTCTGTCCGTCTGAGCCACTATCCGGCGGACCCGGAATTTCTGGAAGCATGCGACTCTCTTGGACTCTATGTTATGGATGAACTGGGTGGCTGGCATGGCAAGTACGACACCCCTACGGGAGTACGTCTGATTGAAGGCATGATAGAACGTGATGTGAACCATCCGTCCATTATCTGGTGGAGCAATGGTAATGAAAAAGGCTGGAACATTGAACTGGACGGAGAATTCCATAAATACGATCTGCAGAAACGCCCGGTCATCCATCCGCAAGGTAACTTCTCCGGTTTCGAAACCATGCACTATCGTTCGTATGGAGAAAGCCAGAACTACATGCGCCTGCCGGAAATCTTTATGCCTACTGAATTCCTGCATGGTTTGTACGACGGAGGTCATGGTGCCGGCCTGTATGATTACTGGGAAATGATGCGTAAACATCCGCGTTGTATCGGTGGTTTCCTGTGGGTATTGGCGGATGAAGGCGTGAAGCGCGTGGATATGGACGGGTTCATAGACAATCAGGGAAATTTCGGAGCTGACGGAATTGTAGGCCCTCACCATGAAAAGGAAGGCAGCTATTACACTATCAAGCAGCTATGGAGCCCGGTGCAGGTTATGAATACCGCTATCGACCGGAATTTCGACGGTAAACTCTCTGTGGAGAACCGTTATGATTATCTGAACCTGAACACCTGTCGTTTTATCTGGCAGCAAGTGAAGTTCCCGTCGGTAACGGATGCTTCCAATACAACTACACGGATTCTGAAACAAGGTGAAGTGCAAGGAAGCGATGTAGCAGCCCATGGAGTGGGAGTGGTGGATATCAAGACTTCTATTCTTCCCGAAGCGGATGCTCTTTTCCTGACAGTTATAGATAAATATGGGTATGAACTTTGGCGCTGGACTTTCCCCGTAGATAAACTGAATCGGGAAACAGAACAGTTTTCTGCATCATCCGGCCGTGTATCCTATACGGAAACAGAAAAAGGTATTACGGTAAAAGCAAACGGGCGTACTTTTGTCTTTTCAAAGAAAGACGGGCAGCTGAAAGATGTATCCGTCAATAACCGTAAGATTAGTTTTGCTAACGGTCCCCGTTTTATCGGTGCACGTCGTGCAGACCGTTCCCTAGATCAGTTCTATAATCATGATGACGAAAAAGCCAAGGCAAAGGACCGTACTTACAGTGAATTTACCGATGCGGCAGTCTTCACGAAACTGGATGTGAAAGAAGAGGGGGGGAATCTGATCCTCACCGCTAATTATAAACTGGGTAATTTAGATAAAGCTCAGTGGACAATTCATCCGGACGGCATGGCTACTCTTGATTATACCTACAACTTCTCCGGTGTGGTAGACCTGATGGGTATTTGCTTTGATTACCCTGAAGAACAAGTGCTCAGCAAGCGTTGGTTGGGAGCAGGTCCGTATCGTGTATGGCAGAATCGTATTCATGGCACGCAGTATGATATCTGGGAGAATGATTATAACGATCCTATTCCGGGTGAGACATTCACCTATCCTGAATTCAAGGGATATTTTGGCAGTGTCTCTTGGATGAGTATTCGCACGAAAGAGGGAACCATCAGCCTGACGAATGAAACACCTGATTCCTATATCGGAGTATATCAACCCCGTGATGGTCGTGACCGGTTACTGTATACACTTCCCGAAAGCGGAATTTCTGTTTTGAATGTAATTCCTCCGGTGCGTAATAAAGTAAATTCCACGGACTTGTGCGGTCCTTCTTCACAACCAAAATGGGTGGATGGCTCGCAAACGGGACGCCTTGTTATCCGGTTTGAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,215 total letters Query= CMR200018.120 Length=3195 Score E Sequences producing significant alignments: (Bits) Value 0_wt 5023 0.0 1_03C_CMR200018.120Seq171C03.seq 1934 0.0 2_C3_CMR200018.120Seq171C09.seq 1849 0.0 Q T L P Q T E R Q Y L S G H G C D D T V E W D F F C T D G R Query_1 202 CAGACACTTCCGCAGACAGAGCGGCAATACCTCTCCGGCCACGGATGCGACGACACAGTAGAATGGGACTTTTTCTGTACCGACGGACGT 291 0 1 .......................................................................................... 90 N S G R W T K I G V P S C W E L Q G F G T Y Q Y G I S F Y G Query_1 292 AACTCCGGTCGATGGACGAAAATAGGCGTCCCCTCTTGCTGGGAGTTGCAGGGTTTTGGTACCTATCAGTATGGAATTAGTTTTTATGGT 381 0 91 .......................................................................................... 180 K A F P E G I A G E K G M Y K Y E F E V P E E F R G K Q V S Query_1 382 AAAGCCTTTCCCGAAGGCATTGCCGGTGAGAAAGGAATGTATAAATATGAGTTTGAAGTTCCCGAGGAATTTCGTGGCAAGCAGGTCAGC 471 0 181 .......................................................................................... 270 1 530 ........... 540 2 1178 .... 1175 L V F E A S M T D T E V K V N G R K A G S K H Q G A F Query_1 472 CTTGTGTT--CGAAG-CATCCATGACCGATACGGAAGTTAAGGTTAACGGACGTAAGG--CAGGAT-CGAAACACCA-GGGAGCCTTCTA 554 0 271 ........--.....-..........................................--......-..........-............ 353 2 1174 ..N.....TCN....N..N...NN-.....NNC.G..N......--...N-.......GC......TN.....C...A............ 1089 Y C F S Y N V T D L L K Y G K K N Q L E V T V S K E S E N Query_1 555 TTGCTTTTCATATAATGTCACGGATTTACTG-AAATATGGCAAGAA-GAATCAGCTGGAAGTAACAGTTTCCAAGGAG-AGTGAGAATGC 641 0 354 ...............................-..............-...............................-........... 440 2 1088 ....................N..........N...NNN........A..........N...GT...............N........... 999 A S V N L A E R R A D Y W N F G G I F R P V F L E V K P A V Query_1 642 CA-GTGTGAATCTTGCCGAACGGCGCGCCGATTATTGGAACTTTGGCGGTATCTTCCGCCCGGTATTTCTGGAAGTAAAACCTGCCGTCA 730 0 441 ..-....................................................................................... 529 0 1017 .....A........... 1033 0 1873 ........... 1883 2 998 ..A..N.......-..............-...A..-...................................................... 912 2 421 .....A........... 405 N L R H I A I D A Q M D G S F R A N C Y T N I S G D G M S Query_1 731 ATCTCCGTCATATTGCTATTGATGCACAAA-TGG-ACGGATCATTCCGTGCCAATTGCTACACGAATATCTCCGGTGACGGAATGAGTAT 818 0 530 ..............................-...-....................................................... 617 2 911 ..N...........................A...N..A.................................................... 822 2 280 ..-...-...... 290 I R A Q I L D G K G K K L A D T T V P L K A G S D W T T L Q Query_1 819 CCGTGCACAGATTTTGGACGGTAAA-GGGAAGAAACTGGCAGATACCACCGTACCCCTAAAAGCCGGAAGCGACTGGACTACTTTACAAT 907 0 618 .........................-................................................................ 706 2 821 .........................A................................................................ 732 L N V S A P A L W T A E T P N L Y K A Q F S L L D K G G K V Query_1 908 TGAACGTTTCTGCCCCTGCCTTATGGACGGCAGAAACTCCGAATCTTTATAAAGCTCAATTTTCACTGTTGGATAAAGGAGGTAAAGTCC 997 0 707 .......................................................................................... 796 2 731 .......................................................................................... 642 L H H E T E T F G F R T I E V R E S D G L Y V N G V R I N V Query_1 998 TGCATCATGAGACCGAGACATTCGGTTTCCGTACTATCGAAGTTCGTGAAAGTGACGGATTGTACGTGAACGGGGTGCGTATCAACGTGC 1087 0 797 .......................................................................................... 886 2 641 .......................................................................................... 552 R G V N R H S F R P E S G R T L S K A K N I E D V L L M K G Query_1 1088 GTGGTGTCAACCGTCATAGTTTCCGTCCCGAAAGCGGTCGTACCCTAAGTAAAGCGAAGAATATTGAAGATGTACTTCTGATGAAGGGCA 1177 0 887 .......................................................................................... 976 0 2652 ............ 2663 2 551 ...................................................................G...................... 462 M N M N S V R L S H Y P A D P E F L E A C D S L G L Y V M D Query_1 1178 TGAATATGAATTCTGTCCGTCTGAGCCACTATCCGGCGGACCCGGAATTTCTGGAAGCATGCGACTCTCTTGGACTCTATGTTATGGATG 1267 0 977 .......................................................................................... 1066 0 498 .....T........... 514 0 2661 ........... 2671 2 461 .......................................................................................... 372 2 943 .....T........... 927 E L G G W H G K Y D T P T G V R L I E G M I E R D V N H P S Query_1 1268 AACTGGGTGGCTGGCATGGCAAGTACGACACCCCTACGGGAGTACGTCTGATTGAAGGCATGATAGAACGTGATGTGAACCATCCGTCCA 1357 0 1067 .......................................................................................... 1156 0 568 ......... 560 2 371 .......................................................................................... 282 I I W W S N G N Q K G W N I E L D G E F H K Y D L Q K R P V Query_1 1358 TTATCTGGTGGAGCAATGGTAATCAGAAAGGCTGGAACATTGAACTGGACGGAGAATTCCATAAATACGATCTGCAGAAACGCCCGGTCA 1447 0 1157 .......................G.A................................................................ 1246 0 559 .. 558 1 13 ...........-..................................................... 76 2 281 .......................G.A................................................................ 192 I H P Q G N F S G F E T M H Y R S Y G E S Q N Y M R L P E I Query_1 1448 TCCATCCGCAAGGTAACTTCTCCGGTTTCGAAACCATGCACTATCGTTCGTATGGAGAAAGCCAGAACTACATGCGCCTGCCGGAAATCT 1537 0 1247 .......................................................................................... 1336 0 1281 ............ 1270 0 2323 ............ 2334 1 77 .......................................................................................... 166 1 111 ............ 100 2 191 .......................................................................................... 102 2 157 ............ 168 F M P T E F L H G L Y D G G H G A G L Y D Y W E M M R K H P Query_1 1538 TTATGCCTACTGAATTCCTGCATGGTTTGTACGACGGAGGTCATGGTGCCGGCCTGTATGATTACTGGGAAATGATGCGTAAACATCCGC 1627 0 1337 .......................................................................................... 1426 1 167 ...........C.G............................................................................ 256 2 101 ...........C.G............................................................................ 12 R C I G G F L W V L A D E G V K R V D M D G F I D N Q G N F Query_1 1628 GTTGTATCGGTGGTTTCCTGTGGGTATTGGCGGATGAAGGCGTGAAGCGCGTGGATATGGACGGGTTCATAGACAATCAGGGAAATTTCG 1717 0 1427 .......................................................................................... 1516 1 257 .......................................................................................... 346 2 11 .-..... 6 G A D G I V G P H H E K E G S Y Y T I K Q L W S P V Q V M N Query_1 1718 GAGCTGACGGAATTGTAGGCCCTCACCATGAAAAGGAAGGCAGCTATTACACTATCAAGCAGCTATGGAGCCCGGTGCAGGTTATGAATA 1807 0 1517 .......................................................................................... 1606 1 347 .......................................................................................... 436 T A I D R N F D G K L S V E N R Y D Y L N L N T C R F I W Q Query_1 1808 CCGCTATCGACCGGAATTTCGACGGTAAACTCTCTGTGGAGAACCGTTATGATTATCTGAACCTGAACACCTGTCGTTTTATCTGGCAGC 1897 0 1607 .......................................................................................... 1696 1 437 .......................................................................................... 526 Q V K F P S V T D A S N T T T R I L K Q G E V Q G S D V A A Query_1 1898 AAGTGAAGTTCCCGTCGGTAACGGATGCTTCCAATACAACTACACGGATTCTGAAACAAGGTGAAGTGCAAGGAAGCGATGTAGCAGCCC 1987 0 1697 .......................................................................................... 1786 0 234 ........... 244 1 527 .......................................................................................... 616 H G V G V V D I K T S I L P E A D A L F L T V I D K Y G Y E Query_1 1988 ATGGAGTGGGAGTGGTGGATATCAAGACTTCTATTCTTCCCGAAGCGGATGCTCTTTTCCTGACAGTTATAGATAAATATGGGTATGAAC 2077 0 1787 .......................................................................................... 1876 0 477 .... 480 1 617 .......................................................................................... 706 2 964 .... 961 L W R W T F P V D K L N R E T E Q F S A S S G R V S Y T E T Query_1 2078 TTTGGCGCTGGACTTTCCCCGTAGATAAACTGAATCGGGAAACAGAACAGTTTTCTGCATCATCCGGCCGTGTATCCTATACGGAAACAG 2167 0 1877 .......................................................................................... 1966 0 481 ....... 487 1 707 .......................................................................................... 796 2 960 ....... 954 E K G I T V K A N G R T F V F S K K D G Q L K D V S V N N R Query_1 2168 AAAAAGGTATTACGGTAAAAGCAAACGGGCGTACTTTTGTCTTTTCAAAGAAAGACGGGCAGCTGAAAGATGTATCCGTCAATAACCGTA 2257 0 1967 .......................................................................................... 2056 1 797 .......................................................................................... 886 K I S F A N G P R F I G A R R A D R S L D Q F Y N H D D E K Query_1 2258 AGATTAGTTTTGCTAACGGTCCCCGTTTTATCGGTGCACGTCGTGCAGACCGTTCCCTAGATCAGTTCTATAATCATGATGACGAAAAAG 2347 0 2057 .......................................................................................... 2146 1 887 .......................................................................................... 976 A K A K D R T Y S E F T D A A V F T K L D V K E E G G N L I Query_1 2348 CCAAGGCAAAGGACCGTACTTACAGTGAATTTACCGATGCGGCAGTCTTCACGAAACTGGATGTGAAAGAAGAgggggggAATCTGATCC 2437 0 2147 .......................................................................................... 2236 1 977 ..........................TG.............................................-......N......... 1065 L T A N Y K L G N L D K A Q W T I H P D G M A T L D Y T Y Query_1 2438 TCACCGCTAATTATAAACTGGGTAATTTAG-ATAAAGCTCAGTGGACAA-TTCATCCGGACGGCATGGCTACTCTTGATTATACCTACAA 2525 0 2237 ..............................-..................-........................................ 2324 0 1261 .. 1262 1 1066 ........T..--NT....-..N.T..A..A..................T..........NC............-....--NNN...... 1149 1 91 .. 92 2 177 .. 176 N F S G V V D L M G I C F D Y P E E Q V L S K R W L G A G P Query_1 2526 CTTCTCCGGTGTGGTAGACCTGATGGGTATTTGCTTTGATTACCCTGAAGAACAAGTGCTCAGCAAGCGTTGGTTGGGAGCAGGTCCGTA 2615 0 2325 .......................................................................................... 2414 0 1263 .......... 1272 1 1150 .C.NCT....... 1162 1 93 .......... 102 2 175 .......... 166 Y R V W Q N R I H G T Q Y D I W E N D Y N D P I P G E T F T Query_1 2616 TCGTGTATGGCAGAATCGTATTCATGGCACGCAGTATGATATCTGGGAGAATGATTATAACGATCCTATTCCGGGTGAGACATTCACCTA 2705 0 2415 .......................................................................................... 2504 Y P E F K G Y F G S V S W M S I R T K E G T I S L T N E T P Query_1 2706 TCCTGAATTCAAGGGATATTTTGGCAGTGTCTCTTGGATGAGTATTCGCACGAAAGAGGGAACCATCAGCCTGACGAATGAAACACCTGA 2795 0 2505 .......................................................................................... 2594 D S Y I G V Y Q P R D G R D R L L Y T L P E S G I S V L N V Query_1 2796 TTCCTATATCGGAGTATATCAACCCCGTGATGGTCGTGACCGGTTACTGTATACACTTCCCGAAAGCGGAATTTCTGTTTTGAATGTAAT 2885 0 2595 .......................................................................................... 2684 0 912 ............ 923 0 1019 ........... 1029 2 526 ............ 515 2 419 ........... 409 I P P V R N K V N S T D L C G P S S Q P K W V D G S Q T G R Query_1 2886 TCCTCCGGTGCGTAATAAAGTAAATTCCACGGACTTGTGCGGTCCTTCTTCACAACCAAAATGGGTGGATGGCTCGCAAACGGGACGCCT 2975 0 2685 .......................................................................................... 2774 L V I R F E Query_1 2976 TGTTATCCGGTTTGAA 2991 0 2775 ................ 2790 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 16396497 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 5,215 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200018.121 (CMR200018.20-949.P(949).E469Q.pMCSG68)
QTLPQTERQYLSGHGCDDTVEWDFFCTDGRNSGRWTKIGVPSCWELQGFGTYQYGISFYGKAFPEGIAGEKGMYKYEFEVPEEFRGKQVSLVFEASMTDTEVKVNGRKAGSKHQGAFYCFSYNVTDLLKYGKKNQLEVTVSKESENASVNLAERRADYWNFGGIFRPVFLEVKPAVNLRHIAIDAQMDGSFRANCYTNISGDGMSIRAQILDGKGKKLADTTVPLKAGSDWTTLQLNVSAPALWTAETPNLYKAQFSLLDKGGKVLHHETETFGFRTIEVRESDGLYVNGVRINVRGVNRHSFRPESGRTLSKAKNIEDVLLMKGMNMNSVRLSHYPADPEFLEACDSLGLYVMDELGGWHGKYDTPTGVRLIEGMIERDVNHPSIIWWSNGNEKGWNIELDGEFHKYDLQKRPVIHPQGNFSGFETMHYRSYGESQNYMRLPEIFMPTEFLHGLYDGGHGAGLYDYWEMMRKHPRCIGGFLWVLADEGVKRVDMDGFIDNQGNFGADGIVGPHHEKEGSYYTIKQLWSPVQVMNTAIDRNFDGKLSVENRYDYLNLNTCRFIWQQVKFPSVTDASNTTTRILKQGEVQGSDVAAHGVGVVDIKTSILPEADALFLTVIDKYGYELWRWTFPVDKLNRETEQFSASSGRVSYTETEKGITVKANGRTFVFSKKDGQLKDVSVNNRKISFANGPRFIGARRADRSLDQFYNHDDEKAKAKDRTYSEFTDAAVFTKLDVKEEGGNLILTANYKLGNLDKAQWTIHPDGMATLDYTYNFSGVVDLMGICFDYPEEQVLSKRWLGAGPYRVWQNRIHGTQYDIWENDYNDPIPGETFTYPEFKGYFGSVSWMSIRTKEGTISLTNETPDSYIGVYQPRDGRDRLLYTLPESGISVLNVIPPVRNKVNSTDLCGPSSQPKWVDGSQTGRLVIRFE
CAGACACTTCCGCAGACAGAGCGGCAATACCTCTCCGGCCACGGATGCGACGACACAGTAGAATGGGACTTTTTCTGTACCGACGGACGTAACTCCGGTCGATGGACGAAAATAGGCGTCCCCTCTTGCTGGGAGTTGCAGGGTTTTGGTACCTATCAGTATGGAATTAGTTTTTATGGTAAAGCCTTTCCCGAAGGCATTGCCGGTGAGAAAGGAATGTATAAATATGAGTTTGAAGTTCCCGAGGAATTTCGTGGCAAGCAGGTCAGCCTTGTGTTCGAAGCATCCATGACCGATACGGAAGTTAAGGTTAACGGACGTAAGGCAGGATCGAAACACCAGGGAGCCTTCTATTGCTTTTCATATAATGTCACGGATTTACTGAAATATGGCAAGAAGAATCAGCTGGAAGTAACAGTTTCCAAGGAGAGTGAGAATGCCAGTGTGAATCTTGCCGAACGGCGCGCCGATTATTGGAACTTTGGCGGTATCTTCCGCCCGGTATTTCTGGAAGTAAAACCTGCCGTCAATCTCCGTCATATTGCTATTGATGCACAAATGGACGGATCATTCCGTGCCAATTGCTACACGAATATCTCCGGTGACGGAATGAGTATCCGTGCACAGATTTTGGACGGTAAAGGGAAGAAACTGGCAGATACCACCGTACCCCTAAAAGCCGGAAGCGACTGGACTACTTTACAATTGAACGTTTCTGCCCCTGCCTTATGGACGGCAGAAACTCCGAATCTTTATAAAGCTCAATTTTCACTGTTGGATAAAGGAGGTAAAGTCCTGCATCATGAGACCGAGACATTCGGTTTCCGTACTATCGAAGTTCGTGAAAGTGACGGATTGTACGTGAACGGGGTGCGTATCAACGTGCGTGGTGTCAACCGTCATAGTTTCCGTCCCGAAAGCGGTCGTACCCTAAGTAAAGCGAAGAATATTGAAGATGTACTTCTGATGAAGGGCATGAATATGAATTCTGTCCGTCTGAGCCACTATCCGGCGGACCCGGAATTTCTGGAAGCATGCGACTCTCTTGGACTCTATGTTATGGATGAACTGGGTGGCTGGCATGGCAAGTACGACACCCCTACGGGAGTACGTCTGATTGAAGGCATGATAGAACGTGATGTGAACCATCCGTCCATTATCTGGTGGAGCAATGGTAATGAAAAAGGCTGGAACATTGAACTGGACGGAGAATTCCATAAATACGATCTGCAGAAACGCCCGGTCATCCATCCGCAAGGTAACTTCTCCGGTTTCGAAACCATGCACTATCGTTCGTATGGAGAAAGCCAGAACTACATGCGCCTGCCGGAAATCTTTATGCCTACTGAATTCCTGCATGGTTTGTACGACGGAGGTCATGGTGCCGGCCTGTATGATTACTGGGAAATGATGCGTAAACATCCGCGTTGTATCGGTGGTTTCCTGTGGGTATTGGCGGATGAAGGCGTGAAGCGCGTGGATATGGACGGGTTCATAGACAATCAGGGAAATTTCGGAGCTGACGGAATTGTAGGCCCTCACCATGAAAAGGAAGGCAGCTATTACACTATCAAGCAGCTATGGAGCCCGGTGCAGGTTATGAATACCGCTATCGACCGGAATTTCGACGGTAAACTCTCTGTGGAGAACCGTTATGATTATCTGAACCTGAACACCTGTCGTTTTATCTGGCAGCAAGTGAAGTTCCCGTCGGTAACGGATGCTTCCAATACAACTACACGGATTCTGAAACAAGGTGAAGTGCAAGGAAGCGATGTAGCAGCCCATGGAGTGGGAGTGGTGGATATCAAGACTTCTATTCTTCCCGAAGCGGATGCTCTTTTCCTGACAGTTATAGATAAATATGGGTATGAACTTTGGCGCTGGACTTTCCCCGTAGATAAACTGAATCGGGAAACAGAACAGTTTTCTGCATCATCCGGCCGTGTATCCTATACGGAAACAGAAAAAGGTATTACGGTAAAAGCAAACGGGCGTACTTTTGTCTTTTCAAAGAAAGACGGGCAGCTGAAAGATGTATCCGTCAATAACCGTAAGATTAGTTTTGCTAACGGTCCCCGTTTTATCGGTGCACGTCGTGCAGACCGTTCCCTAGATCAGTTCTATAATCATGATGACGAAAAAGCCAAGGCAAAGGACCGTACTTACAGTGAATTTACCGATGCGGCAGTCTTCACGAAACTGGATGTGAAAGAAGAGGGGGGGAATCTGATCCTCACCGCTAATTATAAACTGGGTAATTTAGATAAAGCTCAGTGGACAATTCATCCGGACGGCATGGCTACTCTTGATTATACCTACAACTTCTCCGGTGTGGTAGACCTGATGGGTATTTGCTTTGATTACCCTGAAGAACAAGTGCTCAGCAAGCGTTGGTTGGGAGCAGGTCCGTATCGTGTATGGCAGAATCGTATTCATGGCACGCAGTATGATATCTGGGAGAATGATTATAACGATCCTATTCCGGGTGAGACATTCACCTATCCTGAATTCAAGGGATATTTTGGCAGTGTCTCTTGGATGAGTATTCGCACGAAAGAGGGAACCATCAGCCTGACGAATGAAACACCTGATTCCTATATCGGAGTATATCAACCCCGTGATGGTCGTGACCGGTTACTGTATACACTTCCCGAAAGCGGAATTTCTGTTTTGAATGTAATTCCTCCGGTGCGTAATAAAGTAAATTCCACGGACTTGTGCGGTCCTTCTTCACAACCAAAATGGGTGGATGGCTCGCAAACGGGACGCCTTGTTATCCGGTTTGAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,314 total letters Query= CMR200018.121 Length=3195 Score E Sequences producing significant alignments: (Bits) Value 0_wt 5023 0.0 2_D3_CMR200018.121Seq171D09.seq 1673 0.0 1_03D_CMR200018.121Seq171D03.seq 1305 0.0 Q T L P Q T E R Q Y L S G H G C D D T V E W D F F C T D G R Query_1 202 CAGACACTTCCGCAGACAGAGCGGCAATACCTCTCCGGCCACGGATGCGACGACACAGTAGAATGGGACTTTTTCTGTACCGACGGACGT 291 0 1 .......................................................................................... 90 N S G R W T K I G V P S C W E L Q G F G T Y Q Y G I S F Y G Query_1 292 AACTCCGGTCGATGGACGAAAATAGGCGTCCCCTCTTGCTGGGAGTTGCAGGGTTTTGGTACCTATCAGTATGGAATTAGTTTTTATGGT 381 0 91 .......................................................................................... 180 K A F P E G I A G E K G M Y K Y E F E V P E E F R G K Q V S Query_1 382 AAAGCCTTTCCCGAAGGCATTGCCGGTGAGAAAGGAATGTATAAATATGAGTTTGAAGTTCCCGAGGAATTTCGTGGCAAGCAGGTCAGC 471 0 181 .......................................................................................... 270 L V F E A S M T D T E V K V N G R K A G S K H Q G A F Y C F Query_1 472 CTTGTGTTCGAAGCATCCATGACCGATACGGAAGTTAAGGTTAACGGACGTAAGGCAGGATCGAAACACCAGGGAGCCTTCTATTGCTTT 561 0 271 .......................................................................................... 360 2 1135 ....T..........N-......NCGT......G.AG.....G.-.....N.NNN.NNG--..N.N. 1073 S Y N V T D L L K Y G K K N Q L E V T V S K E S E N A S V Query_1 562 TCATATAATGTCACGGATTTACTGAAATATGGCAAGAAGAAT-CAGCTGGAAGTAACAGTTTCCAAGGAGAGTGAGAATGCCAGTGTGAA 650 0 361 ..........................................-............................................... 449 2 1072 .N-..GN.NN..NN..NA..NAN.G...TNN........C..T...N....GN..N-.N...N....N....NN.N...A......N..T 985 N L A E R R A D Y W N F G G I F R P V F L E V K P A V N L R Query_1 651 TCTTGCCGAACGGCGCGCCGATTATTGGAACTTTGGCGGTATCTTCCGCCCGGTATTTCTGGAAGTAAAACCTGCCGTCAATCTCCGTCA 740 0 450 .......................................................................................... 539 0 1017 .....A........... 1033 0 1873 ........... 1883 2 984 ....A.-.....A....A....-...A..........N....N......G....NN..N..-.......-........N........... 899 2 420 .....A........... 404 H I A I D A Q M D G S F R A N C Y T N I S G D G M S I R A Q Query_1 741 TATTGCTATTGATGCACAAATGGACGGATCATTCCGTGCCAATTGCTACACGAATATCTCCGGTGACGGAATGAGTATCCGTGCACAGAT 830 0 540 .......................................................................................... 629 2 898 ........G....AN......................N.....-......N....................................... 810 I L D G K G K K L A D T T V P L K A G S D W T T L Q L N V S Query_1 831 TTTGGACGGTAAAGGGAAGAAACTGGCAGATACCACCGTACCCCTAAAAGCCGGAAGCGACTGGACTACTTTACAA-TTGAACGTTTCTG 919 0 630 ............................................................................-............. 718 2 809 ............................................................................N............. 720 A P A L W T A E T P N L Y K A Q F S L L D K G G K V L H H E Query_1 920 CCCCTGCCTTATGGACGGCAGAAACTCCGAATCTTTATAAA-GCTCAATTTTCACTGTTGGATAAAGGAGGTAAAGTCCTGCATCATGAG 1008 0 719 .........................................-................................................ 807 2 719 .........................................A................................................ 630 T E T F G F R T I E V R E S D G L Y V N G V R I N V R G V N Query_1 1009 ACCGAGACATTCGGTTTCCGTACTATCGAAGTTCGTGAAAGTGACGGATTGTACGTGAACGGGGTGCGTATCAACGTGCGTGGTGTCAAC 1098 0 808 .......................................................................................... 897 2 629 .......................................................................................... 540 R H S F R P E S G R T L S K A K N I E D V L L M K G M N M N Query_1 1099 CGTCATAGTTTCCGTCCCGAAAGCGGTCGTACCCTAAGTAAAGCGAAGAATATTGAAGATGTACTTCTGATGAAGGGCATGAATATGAAT 1188 0 898 .......................................................................................... 987 0 2652 ............ 2663 2 539 ........................................................G................................. 450 S V R L S H Y P A D P E F L E A C D S L G L Y V M D E L G G Query_1 1189 TCTGTCCGTCTGAGCCACTATCCGGCGGACCCGGAATTTCTGGAAGCATGCGACTCTCTTGGACTCTATGTTATGGATGAACTGGGTGGC 1278 0 988 .......................................................................................... 1077 0 498 .....T........... 514 0 2661 ........... 2671 2 449 .......................................................................................... 360 W H G K Y D T P T G V R L I E G M I E R D V N H P S I I W W Query_1 1279 TGGCATGGCAAGTACGACACCCCTACGGGAGTACGTCTGATTGAAGGCATGATAGAACGTGATGTGAACCATCCGTCCATTATCTGGTGG 1368 0 1078 .......................................................................................... 1167 0 568 ........... 558 2 359 .......................................................................................... 270 2 870 ........... 880 S N G N E K G W N I E L D G E F H K Y D L Q K R P V I H P Q Query_1 1369 AGCAATGGTAATGAAAAAGGCTGGAACATTGAACTGGACGGAGAATTCCATAAATACGATCTGCAGAAACGCCCGGTCATCCATCCGCAA 1458 0 1168 .......................................................................................... 1257 2 269 .......................................................................................... 180 1 1 ..................................................................... 69 G N F S G F E T M H Y R S Y G E S Q N Y M R L P E I F M P T Query_1 1459 GGTAACTTCTCCGGTTTCGAAACCATGCACTATCGTTCGTATGGAGAAAGCCAGAACTACATGCGCCTGCCGGAAATCTTTATGCCTACT 1548 0 1258 .......................................................................................... 1347 0 1281 ............ 1270 0 2323 ............ 2334 2 179 .......................................................................................... 90 2 156 ............ 167 1 70 .......................................................................................... 159 1 93 ............ 82 Q F L H G L Y D G G H G A G L Y D Y W E M M R K H P R C I Query_1 1549 CAGTTCCTGCATGGTTTGT-ACGACGGAGGTCATGGTGCCGGCCTGTATGATTACTGGGAAATGATGCGTAAACATCCGCGTTGTATCGG 1637 0 1348 G.A................-...................................................................... 1436 2 89 ...................A.N............................................................ 8 1 160 ...................-...................................................................... 248 G G F L W V L A D E G V K R V D M D G F I D N Q G N F G A D Query_1 1638 TGGTTTCCTGTGGGTATTGGCGGATGAAGGCGTGAAGCGCGTGGATATGGACGGGTTCATAGACAATCAGGGAAATTTCGGAGCTGACGG 1727 0 1437 .......................................................................................... 1526 1 249 .......................................................................................... 338 G I V G P H H E K E G S Y Y T I K Q L W S P V Q V M N T A I Query_1 1728 AATTGTAGGCCCTCACCATGAAAAGGAAGGCAGCTATTACACTATCAAGCAGCTATGGAGCCCGGTGCAGGTTATGAATACCGCTATCGA 1817 0 1527 .......................................................................................... 1616 1 339 .......................................................................................... 428 D R N F D G K L S V E N R Y D Y L N L N T C R F I W Q Q V K Query_1 1818 CCGGAATTTCGACGGTAAACTCTCTGTGGAGAACCGTTATGATTATCTGAACCTGAACACCTGTCGTTTTATCTGGCAGCAAGTGAAGTT 1907 0 1617 .......................................................................................... 1706 0 234 ....... 240 1 429 .......................................................................................... 518 F P S V T D A S N T T T R I L K Q G E V Q G S D V A A H G V Query_1 1908 CCCGTCGGTAACGGATGCTTCCAATACAACTACACGGATTC-TGAAACAAGGTGAAGTGCAAGGAAGCGATGTAGCAGCCCATGGAGTGG 1996 0 1707 .........................................-................................................ 1795 0 241 .... 244 1 519 .........................................T........................N....................... 608 G V V D I K T S I L P E A D A L F L T V I D K Y G Y E L W R Query_1 1997 GAGTGGTGGATATCAAGACTTCTATTCTTCCCGAAGCGGATGCTCTTTTCCTGACAGTTATAGATAAATATGGGTATGAACTTTGGCGCT 2086 0 1796 .......................................................................................... 1885 0 477 ........... 487 1 609 ............................................................N............A............N... 698 W T F P V D K L N R E T E Q F S A S S G R V S Y T E T Query_1 2087 GGACTTTCCCCGTA-GATAAACTGAATCGGG-AAACAG-AACAGTTTT-CTGCAT-CA-TCCGGCC-GTGTATCCTATAC-GGAAA--CA 2166 0 1886 ..............-................-......-.........-......-..-.......-.............-.....--.. 1965 1 699 ..............TN..T.........N..A......A...N.....N......N..T.......TT..........N.N.....ACN. 788 E K G I T V K A N G R T F V F S K K D G Q L K D V S V Query_1 2167 GAAAAAGG--TATTACGGT--AAAAGCAAACGGGCGTAC---TTTTGTCTTTT--CAAAGAAAGACGGGCAGCTGAAAGATGTATCCGTC 2247 0 1966 ........--.........--..................---...........--................................... 2046 1 789 T.......NTNT..N.T.NTA......N.N....GNNC.ACT...........TCA....... 851 N N R K I S F A N G P R F I G A R R A D R S L D Q F Y N H D Query_1 2248 AATAACCGTAAGATTAGTTTTGCTAACGGTCCCCGTTTTATCGGTGCACGTCGTGCAGACCGTTCCCTAGATCAGTTCTATAATCATGAT 2337 0 2047 .......................................................................................... 2136 D E K A K A K D R T Y S E F T D A A V F T K L D V K E E G G Query_1 2338 GACGAAAAAGCCAAGGCAAAGGACCGTACTTACAGTGAATTTACCGATGCGGCAGTCTTCACGAAACTGGATGTGAAAGAAGAggggggg 2427 0 2137 .......................................................................................... 2226 N L I L T A N Y K L G N L D K A Q W T I H P D G M A T L D Y Query_1 2428 AATCTGATCCTCACCGCTAATTATAAACTGGGTAATTTAGATAAAGCTCAGTGGACAATTCATCCGGACGGCATGGCTACTCTTGATTAT 2517 0 2227 .......................................................................................... 2316 T Y N F S G V V D L M G I C F D Y P E E Q V L S K R W L G A Query_1 2518 ACCTACAACTTCTCCGGTGTGGTAGACCTGATGGGTATTTGCTTTGATTACCCTGAAGAACAAGTGCTCAGCAAGCGTTGGTTGGGAGCA 2607 0 2317 .......................................................................................... 2406 0 1261 ............ 1272 2 176 ............ 165 1 73 ............ 84 G P Y R V W Q N R I H G T Q Y D I W E N D Y N D P I P G E T Query_1 2608 GGTCCGTATCGTGTATGGCAGAATCGTATTCATGGCACGCAGTATGATATCTGGGAGAATGATTATAACGATCCTATTCCGGGTGAGACA 2697 0 2407 .......................................................................................... 2496 F T Y P E F K G Y F G S V S W M S I R T K E G T I S L T N E Query_1 2698 TTCACCTATCCTGAATTCAAGGGATATTTTGGCAGTGTCTCTTGGATGAGTATTCGCACGAAAGAGGGAACCATCAGCCTGACGAATGAA 2787 0 2497 .......................................................................................... 2586 T P D S Y I G V Y Q P R D G R D R L L Y T L P E S G I S V L Query_1 2788 ACACCTGATTCCTATATCGGAGTATATCAACCCCGTGATGGTCGTGACCGGTTACTGTATACACTTCCCGAAAGCGGAATTTCTGTTTTG 2877 0 2587 .......................................................................................... 2676 0 912 ............ 923 0 1019 ........... 1029 2 525 ............ 514 2 418 ........... 408 N V I P P V R N K V N S T D L C G P S S Q P K W V D G S Q T Query_1 2878 AATGTAATTCCTCCGGTGCGTAATAAAGTAAATTCCACGGACTTGTGCGGTCCTTCTTCACAACCAAAATGGGTGGATGGCTCGCAAACG 2967 0 2677 .......................................................................................... 2766 G R L V I R F E Query_1 2968 GGACGCCTTGTTATCCGGTTTGAA 2991 0 2767 ........................ 2790 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 16711020 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 5,314 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200018.122 (CMR200018.20-949.P(949).R718T.pMCSG68)
QTLPQTERQYLSGHGCDDTVEWDFFCTDGRNSGRWTKIGVPSCWELQGFGTYQYGISFYGKAFPEGIAGEKGMYKYEFEVPEEFRGKQVSLVFEASMTDTEVKVNGRKAGSKHQGAFYCFSYNVTDLLKYGKKNQLEVTVSKESENASVNLAERRADYWNFGGIFRPVFLEVKPAVNLRHIAIDAQMDGSFRANCYTNISGDGMSIRAQILDGKGKKLADTTVPLKAGSDWTTLQLNVSAPALWTAETPNLYKAQFSLLDKGGKVLHHETETFGFRTIEVRESDGLYVNGVRINVRGVNRHSFRPESGRTLSKAKNIEDVLLMKGMNMNSVRLSHYPADPEFLEACDSLGLYVMDELGGWHGKYDTPTGVRLIEGMIERDVNHPSIIWWSNGNEKGWNIELDGEFHKYDLQKRPVIHPQGNFSGFETMHYRSYGESQNYMRLPEIFMPTEFLHGLYDGGHGAGLYDYWEMMRKHPRCIGGFLWVLADEGVKRVDMDGFIDNQGNFGADGIVGPHHEKEGSYYTIKQLWSPVQVMNTAIDRNFDGKLSVENRYDYLNLNTCRFIWQQVKFPSVTDASNTTTRILKQGEVQGSDVAAHGVGVVDIKTSILPEADALFLTVIDKYGYELWRWTFPVDKLNRETEQFSASSGRVSYTETEKGITVKANGRTFVFSKKDGQLKDVSVNNRKISFANGPRFIGARRADRSLDQFYNHDDEKAKAKDRTYSEFTDAAVFTKLDVKEEGGNLILTANYKLGNLDKAQWTIHPDGMATLDYTYNFSGVVDLMGICFDYPEEQVLSKRWLGAGPYRVWQNRIHGTQYDIWENDYNDPIPGETFTYPEFKGYFGSVSWMSIRTKEGTISLTNETPDSYIGVYQPRDGRDRLLYTLPESGISVLNVIPPVRNKVNSTDLCGPSSQPKWVDGSQTGRLVIRFE
CAGACACTTCCGCAGACAGAGCGGCAATACCTCTCCGGCCACGGATGCGACGACACAGTAGAATGGGACTTTTTCTGTACCGACGGACGTAACTCCGGTCGATGGACGAAAATAGGCGTCCCCTCTTGCTGGGAGTTGCAGGGTTTTGGTACCTATCAGTATGGAATTAGTTTTTATGGTAAAGCCTTTCCCGAAGGCATTGCCGGTGAGAAAGGAATGTATAAATATGAGTTTGAAGTTCCCGAGGAATTTCGTGGCAAGCAGGTCAGCCTTGTGTTCGAAGCATCCATGACCGATACGGAAGTTAAGGTTAACGGACGTAAGGCAGGATCGAAACACCAGGGAGCCTTCTATTGCTTTTCATATAATGTCACGGATTTACTGAAATATGGCAAGAAGAATCAGCTGGAAGTAACAGTTTCCAAGGAGAGTGAGAATGCCAGTGTGAATCTTGCCGAACGGCGCGCCGATTATTGGAACTTTGGCGGTATCTTCCGCCCGGTATTTCTGGAAGTAAAACCTGCCGTCAATCTCCGTCATATTGCTATTGATGCACAAATGGACGGATCATTCCGTGCCAATTGCTACACGAATATCTCCGGTGACGGAATGAGTATCCGTGCACAGATTTTGGACGGTAAAGGGAAGAAACTGGCAGATACCACCGTACCCCTAAAAGCCGGAAGCGACTGGACTACTTTACAATTGAACGTTTCTGCCCCTGCCTTATGGACGGCAGAAACTCCGAATCTTTATAAAGCTCAATTTTCACTGTTGGATAAAGGAGGTAAAGTCCTGCATCATGAGACCGAGACATTCGGTTTCCGTACTATCGAAGTTCGTGAAAGTGACGGATTGTACGTGAACGGGGTGCGTATCAACGTGCGTGGTGTCAACCGTCATAGTTTCCGTCCCGAAAGCGGTCGTACCCTAAGTAAAGCGAAGAATATTGAAGATGTACTTCTGATGAAGGGCATGAATATGAATTCTGTCCGTCTGAGCCACTATCCGGCGGACCCGGAATTTCTGGAAGCATGCGACTCTCTTGGACTCTATGTTATGGATGAACTGGGTGGCTGGCATGGCAAGTACGACACCCCTACGGGAGTACGTCTGATTGAAGGCATGATAGAACGTGATGTGAACCATCCGTCCATTATCTGGTGGAGCAATGGTAATGAAAAAGGCTGGAACATTGAACTGGACGGAGAATTCCATAAATACGATCTGCAGAAACGCCCGGTCATCCATCCGCAAGGTAACTTCTCCGGTTTCGAAACCATGCACTATCGTTCGTATGGAGAAAGCCAGAACTACATGCGCCTGCCGGAAATCTTTATGCCTACTGAATTCCTGCATGGTTTGTACGACGGAGGTCATGGTGCCGGCCTGTATGATTACTGGGAAATGATGCGTAAACATCCGCGTTGTATCGGTGGTTTCCTGTGGGTATTGGCGGATGAAGGCGTGAAGCGCGTGGATATGGACGGGTTCATAGACAATCAGGGAAATTTCGGAGCTGACGGAATTGTAGGCCCTCACCATGAAAAGGAAGGCAGCTATTACACTATCAAGCAGCTATGGAGCCCGGTGCAGGTTATGAATACCGCTATCGACCGGAATTTCGACGGTAAACTCTCTGTGGAGAACCGTTATGATTATCTGAACCTGAACACCTGTCGTTTTATCTGGCAGCAAGTGAAGTTCCCGTCGGTAACGGATGCTTCCAATACAACTACACGGATTCTGAAACAAGGTGAAGTGCAAGGAAGCGATGTAGCAGCCCATGGAGTGGGAGTGGTGGATATCAAGACTTCTATTCTTCCCGAAGCGGATGCTCTTTTCCTGACAGTTATAGATAAATATGGGTATGAACTTTGGCGCTGGACTTTCCCCGTAGATAAACTGAATCGGGAAACAGAACAGTTTTCTGCATCATCCGGCCGTGTATCCTATACGGAAACAGAAAAAGGTATTACGGTAAAAGCAAACGGGCGTACTTTTGTCTTTTCAAAGAAAGACGGGCAGCTGAAAGATGTATCCGTCAATAACCGTAAGATTAGTTTTGCTAACGGTCCCCGTTTTATCGGTGCACGTCGTGCAGACCGTTCCCTAGATCAGTTCTATAATCATGATGACGAAAAAGCCAAGGCAAAGGACCGTACTTACAGTGAATTTACCGATGCGGCAGTCTTCACGAAACTGGATGTGAAAGAAGAGGGGGGGAATCTGATCCTCACCGCTAATTATAAACTGGGTAATTTAGATAAAGCTCAGTGGACAATTCATCCGGACGGCATGGCTACTCTTGATTATACCTACAACTTCTCCGGTGTGGTAGACCTGATGGGTATTTGCTTTGATTACCCTGAAGAACAAGTGCTCAGCAAGCGTTGGTTGGGAGCAGGTCCGTATCGTGTATGGCAGAATCGTATTCATGGCACGCAGTATGATATCTGGGAGAATGATTATAACGATCCTATTCCGGGTGAGACATTCACCTATCCTGAATTCAAGGGATATTTTGGCAGTGTCTCTTGGATGAGTATTCGCACGAAAGAGGGAACCATCAGCCTGACGAATGAAACACCTGATTCCTATATCGGAGTATATCAACCCCGTGATGGTCGTGACCGGTTACTGTATACACTTCCCGAAAGCGGAATTTCTGTTTTGAATGTAATTCCTCCGGTGCGTAATAAAGTAAATTCCACGGACTTGTGCGGTCCTTCTTCACAACCAAAATGGGTGGATGGCTCGCAAACGGGACGCCTTGTTATCCGGTTTGAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,068 total letters Query= CMR200018.122 Length=3195 Score E Sequences producing significant alignments: (Bits) Value 0_wt 5023 0.0 2_E3_CMR200018.122Seq171E09.seq 1855 0.0 1_03E_CMR200018.122Seq171E03.seq 1727 0.0 Q T L P Q T E R Q Y L S G H G C D D T V E W D F F C T D G R Query_1 202 CAGACACTTCCGCAGACAGAGCGGCAATACCTCTCCGGCCACGGATGCGACGACACAGTAGAATGGGACTTTTTCTGTACCGACGGACGT 291 0 1 .......................................................................................... 90 N S G R W T K I G V P S C W E L Q G F G T Y Q Y G I S F Y G Query_1 292 AACTCCGGTCGATGGACGAAAATAGGCGTCCCCTCTTGCTGGGAGTTGCAGGGTTTTGGTACCTATCAGTATGGAATTAGTTTTTATGGT 381 0 91 .......................................................................................... 180 K A F P E G I A G E K G M Y K Y E F E V P E E F R G K Q V S Query_1 382 AAAGCCTTTCCCGAAGGCATTGCCGGTGAGAAAGGAATGTATAAATATGAGTTTGAAGTTCCCGAGGAATTTCGTGGCAAGCAGGTCAGC 471 0 181 .......................................................................................... 270 L V F E A S M T D T E V K V N G R K A G S K H Q G A F Y C F Query_1 472 CTTGTGTTCGAAGCATCCATGACCGATACGGAAGTTAAGGTTAACGGACGTAAGGCAGGATCGAAACACCAGGGAGCCTTCTATTGCTTT 561 0 271 .......................................................................................... 360 S Y N V T D L L K Y G K K N Q L E V T V S K E S E N A S V N Query_1 562 TCATATAATGTCACGGATTTACTGAAATATGGCAAGAAGAATCAGCTGGAAGTAACAGTTTCCAAGGAGAGTGAGAATGCCAGTGTGAAT 651 0 361 .......................................................................................... 450 L A E R R A D Y W N F G G I F R P V F L E V K P A V N L R H Query_1 652 CTTGCCGAACGGCGCGCCGATTATTGGAACTTTGGCGGTATCTTCCGCCCGGTATTTCTGGAAGTAAAACCTGCCGTCAATCTCCGTCAT 741 0 451 .......................................................................................... 540 0 1017 .....A........... 1033 0 1873 ........... 1883 2 321 ........... 311 I A I D A Q M D G S F R A N C Y T N I S G D G M S I R A Q I Query_1 742 ATTGCTATTGATGCACAAATGGACGGATCATTCCGTGCCAATTGCTACACGAATATCTCCGGTGACGGAATGAGTATCCGTGCACAGATT 831 0 541 .......................................................................................... 630 L D G K G K K L A D T T V P L K A G S D W T T L Q L N V S A Query_1 832 TTGGACGGTAAAGGGAAGAAACTGGCAGATACCACCGTACCCCTAAAAGCCGGAAGCGACTGGACTACTTTACAATTGAACGTTTCTGCC 921 0 631 .......................................................................................... 720 P A L W T A E T P N L Y K A Q F S L L D K G G K V L H H E T Query_1 922 CCTGCCTTATGGACGGCAGAAACTCCGAATCTTTATAAAGCTCAATTTTCACTGTTGGATAAAGGAGGTAAAGTCCTGCATCATGAGACC 1011 0 721 .......................................................................................... 810 E T F G F R T I E V R E S D G L Y V N G V R I N V R G V N R Query_1 1012 GAGACATTCGGTTTCCGTACTATCGAAGTTCGTGAAAGTGACGGATTGTACGTGAACGGGGTGCGTATCAACGTGCGTGGTGTCAACCGT 1101 0 811 .......................................................................................... 900 H S F R P E S G R T L S K A K N I E D V L L M K G M N M N S Query_1 1102 CATAGTTTCCGTCCCGAAAGCGGTCGTACCCTAAGTAAAGCGAAGAATATTGAAGATGTACTTCTGATGAAGGGCATGAATATGAATTCT 1191 0 901 .......................................................................................... 990 0 2652 ............ 2663 1 678 ............ 689 V R L S H Y P A D P E F L E A C D S L G L Y V M D E L G G W Query_1 1192 GTCCGTCTGAGCCACTATCCGGCGGACCCGGAATTTCTGGAAGCATGCGACTCTCTTGGACTCTATGTTATGGATGAACTGGGTGGCTGG 1281 0 991 .......................................................................................... 1080 0 498 .....T........... 514 0 2661 ........... 2671 2 1160 ......TC.A....N-..NCN.................-...-.N.NNT--........C 1106 1 687 ........... 697 H G K Y D T P T G V R L I E G M I E R D V N H P S I I W W S Query_1 1282 CATGGCAAGTACGACACCCCTACGGGAGTACGTCTGATTGAAGGCATGATAGAACGTGATGTGAACCATCCGTCCATTATCTGGTGGAGC 1371 0 1081 .......................................................................................... 1170 0 568 ........... 558 2 1105 ..........T.NNN--..N.N--.....-...N....-....C......-..........................-......N.-... 1025 N G N E K G W N I E L D G E F H K Y D L Q K R P V I H P Q Query_1 1372 -AATGGTAATGAAAAAGGCTGGAACATTGAACTGGACGGAGAATTCCATAAATACGATCTGCAGAAACGCCCGGTCATCCATCCGCAAGG 1460 0 1171 -......................................................................................... 1259 2 1024 N.............................-.......-..N...........N.................................... 937 G N F S G F E T M H Y R S Y G E S Q N Y M R L P E I F M P T Query_1 1461 TAACTTCTCCGGTTTCGAAA-CCATGCACTATCGTTCGTATGGAGAAAGCCAGAACTACATGCGCCTGCCGGAAATCTTTATGCCTACTG 1549 0 1260 ....................-..................................................................... 1348 0 1281 ..........-.. 1270 0 2323 ............ 2334 2 936 .........N.A........A..................................................................... 847 1 349 ............ 360 E F L H G L Y D G G H G A G L Y D Y W E M M R K H P R C I G Query_1 1550 AATTCCTGCATGGTTTGTACGACGGAGGTCATGGTGCCGGCCTGTATGATTACTGGGAAATGATGCGTAAACATCCGCGTTGTATCGGTG 1639 0 1349 .......................................................................................... 1438 2 846 ..N....................................................................................... 757 G F L W V L A D E G V K R V D M D G F I D N Q G N F G A D G Query_1 1640 GTTTCCTGTGGGTATTGGCGGATGAAGGCGTGAAGCGCGTGGATATGGACGGGTTCATAGACAATCAGGGAAATTTCGGAGCTGACGGAA 1729 0 1439 .......................................................................................... 1528 2 756 .......................................................................................... 667 2 1033 ........... 1043 I V G P H H E K E G S Y Y T I K Q L W S P V Q V M N T A I D Query_1 1730 TTGTAGGCCCTCACCATGAAAAGGAAGGCAGCTATTACACTATCAA-GCAGCTATGGAGCCCGGTGCAGGTTATGAATACCGCTATCGAC 1818 0 1529 ..............................................-........................................... 1617 2 666 ..............................................A........................................... 577 R N F D G K L S V E N R Y D Y L N L N T C R F I W Q Q V K F Query_1 1819 CGGAATTTCGACGGTAAACTCTCTGTGGAGAACCGTTATGATTATCTGAACCTGAACACCTGTCGTTTTATCTGGCAGCAAGTGAAGTTC 1908 0 1618 .......................................................................................... 1707 0 234 ........ 241 2 576 .......................................................................................... 487 P S V T D A S N T T T R I L K Q G E V Q G S D V A A H G V G Query_1 1909 CCGTCGGTAACGGATGCTTCCAATACAACTACACGGATTCTGAAACAAGGTGAAGTGCAAGGAAGCGATGTAGCAGCCCATGGAGTGGGA 1998 0 1708 .......................................................................................... 1797 0 242 ... 244 2 486 .......................................................................................... 397 V V D I K T S I L P E A D A L F L T V I D K Y G Y E L W R W Query_1 1999 GTGGTGGATATCAAGACTTCTATTCTTCCCGAAGCGGATGCTCTTTTCCTGACAGTTATAGATAAATATGGGTATGAACTTTGGCGCTGG 2088 0 1798 .......................................................................................... 1887 0 477 ........... 487 2 396 .......................................................................................... 307 T F P V D K L N R E T E Q F S A S S G R V S Y T E T E K G I Query_1 2089 ACTTTCCCCGTAGATAAACTGAATCGGGAAACAGAACAGTTTTCTGCATCATCCGGCCGTGTATCCTATACGGAAACAGAAAAAGGTATT 2178 0 1888 .......................................................................................... 1977 2 306 .......................................................................................... 217 T V K A N G R T F V F S K K D G Q L K D V S V N N R K I S F Query_1 2179 ACGGTAAAAGCAAACGGGCGTACTTTTGTCTTTTCAAAGAAAGACGGGCAGCTGAAAGATGTATCCGTCAATAACCGTAAGATTAGTTTT 2268 0 1978 .......................................................................................... 2067 2 216 .......................................................................................... 127 1 22 ........................................................................ 93 A N G P R F I G A T R A D R S L D Q F Y N H D D E K A K A K Query_1 2269 GCTAACGGTCCCCGTTTTATCGGTGCAACTCGTGCAGACCGTTCCCTAGATCAGTTCTATAATCATGATGACGAAAAAGCCAAGGCAAAG 2358 0 2068 ...........................CG............................................................. 2157 2 126 .......................................................................................... 37 1 94 .......................................................................................... 183 D R T Y S E F T D A A V F T K L D V K E E G G N L I L T A N Query_1 2359 GACCGTACTTACAGTGAATTTACCGATGCGGCAGTCTTCACGAAACTGGATGTGAAAGAAGAgggggggAATCTGATCCTCACCGCTAAT 2448 0 2158 .......................................................................................... 2247 2 36 ..................-........N.N.GN... 2 1 184 .......................................................................................... 273 Y K L G N L D K A Q W T I H P D G M A T L D Y T Y N F S G V Query_1 2449 TATAAACTGGGTAATTTAGATAAAGCTCAGTGGACAATTCATCCGGACGGCATGGCTACTCTTGATTATACCTACAACTTCTCCGGTGTG 2538 0 2248 .......................................................................................... 2337 0 1261 ............ 1272 1 274 .......................................................................................... 363 V D L M G I C F D Y P E E Q V L S K R W L G A G P Y R V W Q Query_1 2539 GTAGACCTGATGGGTATTTGCTTTGATTACCCTGAAGAACAAGTGCTCAGCAAGCGTTGGTTGGGAGCAGGTCCGTATCGTGTATGGCAG 2628 0 2338 .......................................................................................... 2427 1 364 .......................................................................................... 453 N R I H G T Q Y D I W E N D Y N D P I P G E T F T Y P E F K Query_1 2629 AATCGTATTCATGGCACGCAGTATGATATCTGGGAGAATGATTATAACGATCCTATTCCGGGTGAGACATTCACCTATCCTGAATTCAAG 2718 0 2428 .......................................................................................... 2517 1 454 .......................................................................................... 543 G Y F G S V S W M S I R T K E G T I S L T N E T P D S Y I G Query_1 2719 GGATATTTTGGCAGTGTCTCTTGGATGAGTATTCGCACGAAAGAGGGAACCATCAGCCTGACGAATGAAACACCTGATTCCTATATCGGA 2808 0 2518 .......................................................................................... 2607 1 544 .......................................................................................... 633 V Y Q P R D G R D R L L Y T L P E S G I S V L N V I P P V R Query_1 2809 GTATATCAACCCCGTGATGGTCGTGACCGGTTACTGTATACACTTCCCGAAAGCGGAATTTCTGTTTTGAATGTAATTCCTCCGGTGCGT 2898 0 2608 .......................................................................................... 2697 0 912 ............ 923 0 1019 ........... 1029 1 634 .......................................................................................... 723 N K V N S T D L C G P S S Q P K W V D G S Q T G R L V I R F Query_1 2899 AATAAAGTAAATTCCACGGACTTGTGCGGTCCTTCTTCACAACCAAAATGGGTGGATGGCTCGCAAACGGGACGCCTTGTTATCCGGTTT 2988 0 2698 .......................................................................................... 2787 1 724 ................................................................-......................... 812 E * H W K W I T D P N S S S V D K L A A A L E H H H H H H * Query_1 2989 GAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGA 3078 0 2788 ... 2790 1 813 .....................................................................---.................. 899 D P A A N K A R K E A E L A A A T A E Q * L A * P L G A Query_1 3079 GAT-CCGGCTGCTAACAAAGCCCGAAAGGAAGC-TGAGTTGGC-TGCTGCCACCGCTGAGCAATAACTAG-CATAAC-CCCTTGGGGCCT 3163 1 900 ...N.............................T.........N.......N..................T......N............ 989 S K R V L R G F L L K Query_1 3164 CTAAACGGGTCTTGAGGGGTTTTTTGCTGAAA 3195 1 990 ......-............-............ 1019 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 15929478 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 5,068 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200019.104 (CMR200019.24-602.P(704).E419A.pMCSG68)
REVASFNDGWEFKKGPFSKEALQAVQKWNSDWQEVSIPHTWNADDMQKQASAFYEGVGYYRKKFTFPENMEGKRIFLRFEGVGSYAEVYINGYLAGTHKGAYSAFACEISSQVKFGKENEIIVKADNSSRPDVIPTNHILFGVYGGIYRPVWLVVTETCSIIANDCASPGVYITQKNVSKKSAEVTVKVKVDNATLAPVPLELENAIYDRNGKLVKKHSQNFELTPQGVQNYSSHFRLNNPHLWQGREDPYLYKVVSRLMQNGRVIDEVIQPLGIRKYEIVAGKGFFLNGKKYPMLGVTRHQDWWGLGSALTNKEHDFDLAQIMDIGATTVRFAHYQQSDYIYSRCDTLGLVIWAEIPFVNRVSGQEWDNAHQQMRELIRQSFNHPSIYVWGIHNEVYHPHGYTAALTQSIHDLCKLEDPDRYTVAVNGYGHADHPVNQNADIQGMNRYFGWYERKIQDIKPWIEKMEKEYPWQRLMLTEYGADANIEHQTEILGDALNWTSPFYPETFQTKTHEYQWSIIAQHPYIIASYLWNMFDFAVPTSKRGSVDARNMKGMMTFDRRIKKDSYFWYKANWSKEP
CGTGAGGTAGCATCCTTCAACGACGGTTGGGAATTCAAGAAAGGTCCGTTTTCCAAAGAAGCATTGCAGGCTGTACAGAAATGGAATTCAGACTGGCAGGAGGTATCCATCCCACATACTTGGAATGCGGACGACATGCAAAAGCAAGCCTCCGCTTTCTATGAAGGTGTAGGCTACTACCGCAAAAAGTTCACTTTCCCCGAAAATATGGAAGGCAAGCGCATCTTTCTACGCTTCGAGGGTGTAGGTTCTTATGCCGAAGTATATATCAATGGCTATCTGGCAGGAACACACAAAGGAGCTTACTCTGCTTTTGCATGCGAGATCAGCTCGCAAGTGAAGTTCGGCAAGGAAAACGAAATTATAGTAAAGGCAGACAACTCCTCACGTCCCGATGTTATCCCCACGAACCACATTTTGTTCGGTGTTTATGGAGGTATTTACCGCCCCGTATGGCTGGTTGTTACGGAAACTTGCAGCATCATTGCAAACGACTGTGCCTCTCCGGGAGTGTATATTACCCAGAAAAATGTTTCAAAGAAGTCAGCCGAAGTGACTGTAAAAGTAAAGGTGGACAATGCAACCTTAGCTCCCGTCCCACTGGAACTAGAGAATGCAATATACGACAGGAACGGCAAATTGGTAAAGAAACATAGTCAGAATTTCGAACTTACTCCACAAGGAGTGCAGAACTATTCCTCACACTTCCGACTGAACAATCCCCATTTATGGCAAGGACGTGAAGACCCATATCTGTATAAAGTCGTTTCACGTCTCATGCAGAATGGACGCGTGATAGACGAAGTTATTCAGCCCCTGGGGATTCGCAAGTATGAAATCGTGGCAGGCAAAGGATTCTTCCTCAATGGAAAGAAGTACCCCATGCTGGGGGTAACCCGCCATCAAGACTGGTGGGGACTGGGTAGCGCCCTCACCAATAAAGAGCACGACTTCGACTTGGCACAAATTATGGATATCGGTGCAACCACCGTCCGCTTTGCACATTACCAACAGTCTGACTACATCTATTCACGCTGCGACACGCTGGGATTGGTAATCTGGGCAGAGATCCCCTTCGTGAACCGAGTCAGCGGCCAAGAGTGGGACAATGCCCACCAACAGATGCGCGAACTTATCCGTCAAAGCTTTAATCATCCCTCCATCTATGTATGGGGAATCCATAATGAAGTGTATCATCCGCATGGTTATACCGCCGCACTTACCCAATCCATTCACGACCTCTGCAAACTGGAGGATCCAGACCGCTACACGGTTGCGGTAAATGGTTACGGTCATGCCGATCACCCCGTCAACCAAAATGCGGATATACAGGGCATGAACCGTTACTTCGGTTGGTACGAACGGAAGATACAAGACATCAAGCCATGGATAGAGAAAATGGAAAAAGAATATCCTTGGCAACGCCTGATGCTGACAGAATATGGTGCAGACGCCAATATAGAACATCAGACGGAAATATTGGGCGATGCACTGAATTGGACAAGCCCTTTCTATCCGGAAACATTTCAAACTAAAACCCACGAATACCAATGGAGCATCATTGCCCAACATCCTTACATCATCGCTTCTTACCTGTGGAATATGTTCGACTTTGCAGTGCCAACATCAAAAAGAGGCAGTGTAGACGCCCGCAACATGAAAGGCATGATGACTTTCGATCGTAGAATCAAGAAAGATTCTTATTTCTGGTATAAGGCGAACTGGAGCAAGGAACCG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,233 total letters Query= CMR200019.104 Length=2142 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3124 0.0 1_02A_A5_CMR200019.104_T7term.seq 1251 0.0 2_05A_A5_CMR200019.104_T7S.seq 95.1 2e-22 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 57 ATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTG 146 2 5 ....................................................A.. 59 W S H P Q F E K G T E N L Y F Q S N A R E V A S F N D G W E Query_1 147 GTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCGTGAGGTAGCATCCTTCAACGACGGTTGGGAATT 236 0 1 ................................... 35 F K K G P F S K E A L Q A V Q K W N S D W Q E V S I P H T W Query_1 237 CAAGAAAGGTCCGTTTTCCAAAGAAGCATTGCAGGCTGTACAGAAATGGAATTCAGACTGGCAGGAGGTATCCATCCCACATACTTGGAA 326 0 36 .......................................................................................... 125 N A D D M Q K Q A S A F Y E G V G Y Y R K K F T F P E N M E Query_1 327 TGCGGACGACATGCAAAAGCAAGCCTCCGCTTTCTATGAAGGTGTAGGCTACTACCGCAAAAAGTTCACTTTCCCCGAAAATATGGAAGG 416 0 126 .......................................................................................... 215 0 320 ............ 309 G K R I F L R F E G V G S Y A E V Y I N G Y L A G T H K G A Query_1 417 CAAGCGCATCTTTCTACGCTTCGAGGGTGTAGGTTCTTATGCCGAAGTATATATCAATGGCTATCTGGCAGGAACACACAAAGGAGCTTA 506 0 216 .......................................................................................... 305 Y S A F A C E I S S Q V K F G K E N E I I V K A D N S S R P Query_1 507 CTCTGCTTTTGCATGCGAGATCAGCTCGCAAGTGAAGTTCGGCAAGGAAAACGAAATTATAGTAAAGGCAGACAACTCCTCACGTCCCGA 596 0 306 .......................................................................................... 395 0 146 ............ 135 D V I P T N H I L F G V Y G G I Y R P V W L V V T E T C S I Query_1 597 TGTTATCCCCACGAACCACATTTTGTTCGGTGTTTATGGAGGTATTTACCGCCCCGTATGGCTGGTTGTTACGGAAACTTGCAGCATCAT 686 0 396 .......................................................................................... 485 0 1552 ..G........ 1562 1 553 ..G........ 543 I A N D C A S P G V Y I T Q K N V S K K S A E V T V K V K V Query_1 687 TGCAAACGACTGTGCCTCTCCGGGAGTGTATATTACCCAGAAAAATGTTTCAAAGAAGTCAGCCGAAGTGACTGTAAAAGTAAAGGTGGA 776 0 486 .......................................................................................... 575 0 1563 ... 1565 1 542 ... 540 D N A T L A P V P L E L E N A I Y D R N G K L V K K H S Q N Query_1 777 CAATGCAACCTTAGCTCCCGTCCCACTGGAACTAGAGAATGCAATATACGACAGGAACGGCAAATTGGTAAAGAAACATAGTCAGAATTT 866 0 576 .......................................................................................... 665 F E L T P Q G V Q N Y S S H F R L N N P H L W Q G R E D P Y Query_1 867 CGAACTTACTCCACAAGGAGTGCAGAACTATTCCTCACACTTCCGACTGAACAATCCCCATTTATGGCAAGGACGTGAAGACCCATATCT 956 0 666 .......................................................................................... 755 L Y K V V S R L M Q N G R V I D E V I Q P L G I R K Y E I V Query_1 957 GTATAAAGTCGTTTCACGTCTCATGCAGAATGGACGCGTGATAGACGAAGTTATTCAGCCCCTGGGGATTCGCAAGTATGAAATCGTGGC 1046 0 756 .......................................................................................... 845 A G K G F F L N G K K Y P M L G V T R H Q D W W G L G S A Query_1 1047 AGGCAAAGGATTCTTCCTCAATGGAAAGAAGTACCCCATGCTGGGGGTAACCC--GCCAT--CAAGACTGGTGGGGACTGGGTAGCGCCC 1132 0 846 .....................................................--.....--............................ 931 1 1223 ....T...N..CN.....TA........-....N..N.N--...NN.T 1179 L T N K E H D F D L A Q I M D I G A T T V R F A H Y Q Q S Query_1 1133 TCACCAATAAAGAGCACGACTTCGACTTGGCACA-AATTATGGATATCGGTGCAACCACCGTCCGCTTTGCACATTACCAACAGTCT--G 1219 0 932 ..................................-....................................................--. 1018 1 1178 .AC.......-....CN....-..N.........N.......A......NNNNNN..N.-..N................N.......TGN 1092 D Y I Y S R C D T L G L V I W A E I P F V N R V S G Q E Query_1 1220 ACTAC--ATCTA-TTCACGCTGCGACACGCTGGGATTGGTAATCTGGGCAGAG-ATCCCCTTCGTGAACCGAGTCAGCGGCCAAGAGT-- 1303 0 1019 .....--.....-........................................-..................................-- 1102 1 1091 NN..ACA.....A.............N......-.............-....NN.NT........N.-...................GTG 1005 W D N A H Q Q M R E L I R Q S F N H P S I Y V W G I H N A V Query_1 1304 GGGACAATGCCCACCAACAGATGCGCGAACTTATCCGTCAAAGCTTTAATCATCCCTCC-ATCTATGTATGGGGAATCCATAATGCTGTG 1392 0 1103 ...........................................................-.........................AA... 1191 1 1004 ....N..N.N......C..........................................C.............................. 915 Y H P H G Y T A A L T Q S I H D L C K L E D P D R Y T V A Query_1 1393 TATCATCCGCATGGTTATACCGCCGCACTTACCCAATCCATTCACGACCTCTGCAAACTGG-AGGATCCAGACCGCTACACGGTTGCGGT 1481 0 1192 .............................................................-............................ 1280 1 914 .............................................................R............................ 825 V N G Y G H A D H P V N Q N A D I Q G M N R Y F G W Y E R K Query_1 1482 AAATGGTTACGGTCATGCCGATCACCCCGTCAACCAAAATGCGGATATACAGGGCATGAACCGTTACTTCGGTTGGTACGAACGGAAGAT 1571 0 1281 .......................................................................................... 1370 1 824 .......................................................................................... 735 I Q D I K P W I E K M E K E Y P W Q R L M L T E Y G A D A N Query_1 1572 ACAAGACATCAAGCCATGGATAGAGAAAATGGAAAAAGAATATCCTTGGCAACGCCTGATGCTGACAGAATATGGTGCAGACGCCAATAT 1661 0 1371 .......................................................................................... 1460 1 734 .......................................................................................... 645 I E H Q T E I L G D A L N W T S P F Y P E T F Q T K T H E Y Query_1 1662 AGAACATCAGACGGAAATATTGGGCGATGCACTGAATTGGACAAGCCCTTTCTATCCGGAAACATTTCAAACTAAAACCCACGAATACCA 1751 0 1461 .......................................................................................... 1550 1 644 .......................................................................................... 555 Q W S I I A Q H P Y I I A S Y L W N M F D F A V P T S K R G Query_1 1752 ATGGAGCATCATTGCCCAACATCCTTACATCATCGCTTCTTACCTGTGGAATATGTTCGACTTTGCAGTGCCAACATCAAAAAGAGGCAG 1841 0 1551 .......................................................................................... 1640 0 475 ..C........... 488 1 554 .......................................................................................... 465 S V D A R N M K G M M T F D R R I K K D S Y F W Y K A N W S Query_1 1842 TGTAGACGCCCGCAACATGAAAGGCATGATGACTTTCGATCGTAGAATCAAGAAAGATTCTTATTTCTGGTATAAGGCGAACTGGAGCAA 1931 0 1641 .......................................................................................... 1730 1 464 .......................................................................................... 375 K E P * H W K W I T D P N S S S V D K L A A A L E H H H H H Query_1 1932 GGAACCGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccacca 2021 0 1731 ....... 1737 1 374 ....... 368 1 62 .....................--..... 37 H * D P A A N K A R K E Query_1 2022 cTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGC 2057 1 41 .........................-..... 12 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 8886750 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,233 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200019.106 (CMR200019.24-602.P(704).E419Q.pMCSG68)
REVASFNDGWEFKKGPFSKEALQAVQKWNSDWQEVSIPHTWNADDMQKQASAFYEGVGYYRKKFTFPENMEGKRIFLRFEGVGSYAEVYINGYLAGTHKGAYSAFACEISSQVKFGKENEIIVKADNSSRPDVIPTNHILFGVYGGIYRPVWLVVTETCSIIANDCASPGVYITQKNVSKKSAEVTVKVKVDNATLAPVPLELENAIYDRNGKLVKKHSQNFELTPQGVQNYSSHFRLNNPHLWQGREDPYLYKVVSRLMQNGRVIDEVIQPLGIRKYEIVAGKGFFLNGKKYPMLGVTRHQDWWGLGSALTNKEHDFDLAQIMDIGATTVRFAHYQQSDYIYSRCDTLGLVIWAEIPFVNRVSGQEWDNAHQQMRELIRQSFNHPSIYVWGIHNEVYHPHGYTAALTQSIHDLCKLEDPDRYTVAVNGYGHADHPVNQNADIQGMNRYFGWYERKIQDIKPWIEKMEKEYPWQRLMLTEYGADANIEHQTEILGDALNWTSPFYPETFQTKTHEYQWSIIAQHPYIIASYLWNMFDFAVPTSKRGSVDARNMKGMMTFDRRIKKDSYFWYKANWSKEP
CGTGAGGTAGCATCCTTCAACGACGGTTGGGAATTCAAGAAAGGTCCGTTTTCCAAAGAAGCATTGCAGGCTGTACAGAAATGGAATTCAGACTGGCAGGAGGTATCCATCCCACATACTTGGAATGCGGACGACATGCAAAAGCAAGCCTCCGCTTTCTATGAAGGTGTAGGCTACTACCGCAAAAAGTTCACTTTCCCCGAAAATATGGAAGGCAAGCGCATCTTTCTACGCTTCGAGGGTGTAGGTTCTTATGCCGAAGTATATATCAATGGCTATCTGGCAGGAACACACAAAGGAGCTTACTCTGCTTTTGCATGCGAGATCAGCTCGCAAGTGAAGTTCGGCAAGGAAAACGAAATTATAGTAAAGGCAGACAACTCCTCACGTCCCGATGTTATCCCCACGAACCACATTTTGTTCGGTGTTTATGGAGGTATTTACCGCCCCGTATGGCTGGTTGTTACGGAAACTTGCAGCATCATTGCAAACGACTGTGCCTCTCCGGGAGTGTATATTACCCAGAAAAATGTTTCAAAGAAGTCAGCCGAAGTGACTGTAAAAGTAAAGGTGGACAATGCAACCTTAGCTCCCGTCCCACTGGAACTAGAGAATGCAATATACGACAGGAACGGCAAATTGGTAAAGAAACATAGTCAGAATTTCGAACTTACTCCACAAGGAGTGCAGAACTATTCCTCACACTTCCGACTGAACAATCCCCATTTATGGCAAGGACGTGAAGACCCATATCTGTATAAAGTCGTTTCACGTCTCATGCAGAATGGACGCGTGATAGACGAAGTTATTCAGCCCCTGGGGATTCGCAAGTATGAAATCGTGGCAGGCAAAGGATTCTTCCTCAATGGAAAGAAGTACCCCATGCTGGGGGTAACCCGCCATCAAGACTGGTGGGGACTGGGTAGCGCCCTCACCAATAAAGAGCACGACTTCGACTTGGCACAAATTATGGATATCGGTGCAACCACCGTCCGCTTTGCACATTACCAACAGTCTGACTACATCTATTCACGCTGCGACACGCTGGGATTGGTAATCTGGGCAGAGATCCCCTTCGTGAACCGAGTCAGCGGCCAAGAGTGGGACAATGCCCACCAACAGATGCGCGAACTTATCCGTCAAAGCTTTAATCATCCCTCCATCTATGTATGGGGAATCCATAATGAAGTGTATCATCCGCATGGTTATACCGCCGCACTTACCCAATCCATTCACGACCTCTGCAAACTGGAGGATCCAGACCGCTACACGGTTGCGGTAAATGGTTACGGTCATGCCGATCACCCCGTCAACCAAAATGCGGATATACAGGGCATGAACCGTTACTTCGGTTGGTACGAACGGAAGATACAAGACATCAAGCCATGGATAGAGAAAATGGAAAAAGAATATCCTTGGCAACGCCTGATGCTGACAGAATATGGTGCAGACGCCAATATAGAACATCAGACGGAAATATTGGGCGATGCACTGAATTGGACAAGCCCTTTCTATCCGGAAACATTTCAAACTAAAACCCACGAATACCAATGGAGCATCATTGCCCAACATCCTTACATCATCGCTTCTTACCTGTGGAATATGTTCGACTTTGCAGTGCCAACATCAAAAAGAGGCAGTGTAGACGCCCGCAACATGAAAGGCATGATGACTTTCGATCGTAGAATCAAGAAAGATTCTTATTTCTGGTATAAGGCGAACTGGAGCAAGGAACCG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,154 total letters Query= CMR200019.106 Length=2142 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3124 0.0 1_02B_B5_CMR200019.106_T7term.seq 1169 0.0 2_05B_B5_CMR200019.106_T7S.seq 89.7 9e-21 Q F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 55 CAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTG 144 2 7 ..T.....T.............................................A.. 63 W S H P Q F E K G T E N L Y F Q S N A R E V A S F N D G W E Query_1 145 TGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCGTGAGGTAGCATCCTTCAACGACGGTTGGGAA 234 0 1 ................................. 33 F K K G P F S K E A L Q A V Q K W N S D W Q E V S I P H T W Query_1 235 TTCAAGAAAGGTCCGTTTTCCAAAGAAGCATTGCAGGCTGTACAGAAATGGAATTCAGACTGGCAGGAGGTATCCATCCCACATACTTGG 324 0 34 .......................................................................................... 123 N A D D M Q K Q A S A F Y E G V G Y Y R K K F T F P E N M E Query_1 325 AATGCGGACGACATGCAAAAGCAAGCCTCCGCTTTCTATGAAGGTGTAGGCTACTACCGCAAAAAGTTCACTTTCCCCGAAAATATGGAA 414 0 124 .......................................................................................... 213 0 320 ............ 309 G K R I F L R F E G V G S Y A E V Y I N G Y L A G T H K G A Query_1 415 GGCAAGCGCATCTTTCTACGCTTCGAGGGTGTAGGTTCTTATGCCGAAGTATATATCAATGGCTATCTGGCAGGAACACACAAAGGAGCT 504 0 214 .......................................................................................... 303 Y S A F A C E I S S Q V K F G K E N E I I V K A D N S S R P Query_1 505 TACTCTGCTTTTGCATGCGAGATCAGCTCGCAAGTGAAGTTCGGCAAGGAAAACGAAATTATAGTAAAGGCAGACAACTCCTCACGTCCC 594 0 304 .......................................................................................... 393 0 146 ............ 135 D V I P T N H I L F G V Y G G I Y R P V W L V V T E T C S I Query_1 595 GATGTTATCCCCACGAACCACATTTTGTTCGGTGTTTATGGAGGTATTTACCGCCCCGTATGGCTGGTTGTTACGGAAACTTGCAGCATC 684 0 394 .......................................................................................... 483 0 1552 ..G...... 1560 1 557 ..G...... 549 I A N D C A S P G V Y I T Q K N V S K K S A E V T V K V K V Query_1 685 ATTGCAAACGACTGTGCCTCTCCGGGAGTGTATATTACCCAGAAAAATGTTTCAAAGAAGTCAGCCGAAGTGACTGTAAAAGTAAAGGTG 774 0 484 .......................................................................................... 573 0 1561 ..... 1565 1 548 ..... 544 D N A T L A P V P L E L E N A I Y D R N G K L V K K H S Q N Query_1 775 GACAATGCAACCTTAGCTCCCGTCCCACTGGAACTAGAGAATGCAATATACGACAGGAACGGCAAATTGGTAAAGAAACATAGTCAGAAT 864 0 574 .......................................................................................... 663 F E L T P Q G V Q N Y S S H F R L N N P H L W Q G R E D P Y Query_1 865 TTCGAACTTACTCCACAAGGAGTGCAGAACTATTCCTCACACTTCCGACTGAACAATCCCCATTTATGGCAAGGACGTGAAGACCCATAT 954 0 664 .......................................................................................... 753 L Y K V V S R L M Q N G R V I D E V I Q P L G I R K Y E I V Query_1 955 CTGTATAAAGTCGTTTCACGTCTCATGCAGAATGGACGCGTGATAGACGAAGTTATTCAGCCCCTGGGGATTCGCAAGTATGAAATCGTG 1044 0 754 .......................................................................................... 843 A G K G F F L N G K K Y P M L G V T R H Q D W W G L G S A L Query_1 1045 GCAGGCAAAGGATTCTTCCTCAATGGAAAGAAGTACCCCATGCTGGGGGTAACCCGCCATCAAGACTGGTGGGGACTGGGTAGCGCCCTC 1134 0 844 .......................................................................................... 933 T N K E H D F D L A Q I M D I G A T T V R F A H Y Q Q S D Query_1 1135 ACCAATAAAGAGCACGACTTCGACTTGGCACAAATTATGGATATCGGTGCAACCACCG-TCCGCTTTGCACATTACCAACAGT-CTGACT 1222 0 934 ..........................................................-........................-...... 1021 1 1158 .....NN...N..............N.N.NG.NNN--....N.T.......NC...-...NN....T..N.-. 1090 Y I Y S R C D T L G L V I W A E I P F V N R V S G Q E W D Query_1 1223 ACATCTATT-CACGCTGCGACACGCTGGGATTGGTAATCTGGGCAGAGATCCCCTTCGTGAACCGA-GTC--AGCGGCCAAGAGTGGGAC 1308 0 1022 .........-........................................................-...--.................. 1107 1 1089 .....N...T.NNNT.....NNN.....-.........N.N.-..........-......NN....A...CAN...N............. 1003 N A H Q Q M R E L I R Q S F N H P S I Y V W G I H N Q V Y Query_1 1309 AATGCCCACCAACAGA-TGCG-CGAACTTATCCGTCAAAGCTTTAATCATCCCTCCATCTATGTATGGGGAATCCATAATCAGGTGTATC 1396 0 1108 ................-....-..........................................................G.A....... 1195 1 1002 ...N...NNN.-....N....G...................N................................................ 914 H P H G Y T A A L T Q S I H D L C K L E D P D R Y T V A V N Query_1 1397 ATCCGCATGGTTA-TACCGCCGCACTTACCCAATCCATTCACGACCTCTGCAAACTGGAGGATCCAGACCGCTACACGGTTGCGGTAAAT 1485 0 1196 .............-............................................................................ 1284 1 913 .............N..................-......................................................... 825 G Y G H A D H P V N Q N A D I Q G M N R Y F G W Y E R K I Q Query_1 1486 GGTTACGGTCATGCCGATCACCCCGTCAACCAAAATGCGGATATACAGGGCATGAACCGTTACTTCGGTTGGTACGAACGGAAGATACAA 1575 0 1285 .......................................................................................... 1374 1 824 .......................................................................................... 735 D I K P W I E K M E K E Y P W Q R L M L T E Y G A D A N I E Query_1 1576 GACATCAAGCCATGGATAGAGAAAATGGAAAAAGAATATCCTTGGCAACGCCTGATGCTGACAGAATATGGTGCAGACGCCAATATAGAA 1665 0 1375 .......................................................................................... 1464 1 734 .......................................................................................... 645 H Q T E I L G D A L N W T S P F Y P E T F Q T K T H E Y Q W Query_1 1666 CATCAGACGGAAATATTGGGCGATGCACTGAATTGGACAAGCCCTTTCTATCCGGAAACATTTCAAACTAAAACCCACGAATACCAATGG 1755 0 1465 .......................................................................................... 1554 0 475 ..C 477 1 644 .......................................................................................... 555 S I I A Q H P Y I I A S Y L W N M F D F A V P T S K R G S V Query_1 1756 AGCATCATTGCCCAACATCCTTACATCATCGCTTCTTACCTGTGGAATATGTTCGACTTTGCAGTGCCAACATCAAAAAGAGGCAGTGTA 1845 0 1555 .......................................................................................... 1644 0 478 ........... 488 1 554 .......................................................................................... 465 D A R N M K G M M T F D R R I K K D S Y F W Y K A N W S K E Query_1 1846 GACGCCCGCAACATGAAAGGCATGATGACTTTCGATCGTAGAATCAAGAAAGATTCTTATTTCTGGTATAAGGCGAACTGGAGCAAGGAA 1935 0 1645 .......................................................................................... 1734 1 464 .......................................................................................... 375 P * H W K W I T D P N S S S V D K L A A A L E H H H H H H * Query_1 1936 CCGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGA 2025 0 1735 ... 1737 1 374 ... 372 1 66 .....................--..... 41 D P A A N K A R Query_1 2026 GATCCGGCTGCTAACAAAG-CCCGAAA 2051 1 45 ..................C....... 20 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 8718875 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,154 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200019.107 (CMR200019.24-602.P(704).E503Q.pMCSG68)
REVASFNDGWEFKKGPFSKEALQAVQKWNSDWQEVSIPHTWNADDMQKQASAFYEGVGYYRKKFTFPENMEGKRIFLRFEGVGSYAEVYINGYLAGTHKGAYSAFACEISSQVKFGKENEIIVKADNSSRPDVIPTNHILFGVYGGIYRPVWLVVTETCSIIANDCASPGVYITQKNVSKKSAEVTVKVKVDNATLAPVPLELENAIYDRNGKLVKKHSQNFELTPQGVQNYSSHFRLNNPHLWQGREDPYLYKVVSRLMQNGRVIDEVIQPLGIRKYEIVAGKGFFLNGKKYPMLGVTRHQDWWGLGSALTNKEHDFDLAQIMDIGATTVRFAHYQQSDYIYSRCDTLGLVIWAEIPFVNRVSGQEWDNAHQQMRELIRQSFNHPSIYVWGIHNEVYHPHGYTAALTQSIHDLCKLEDPDRYTVAVNGYGHADHPVNQNADIQGMNRYFGWYERKIQDIKPWIEKMEKEYPWQRLMLTEYGADANIEHQTEILGDALNWTSPFYPETFQTKTHEYQWSIIAQHPYIIASYLWNMFDFAVPTSKRGSVDARNMKGMMTFDRRIKKDSYFWYKANWSKEP
CGTGAGGTAGCATCCTTCAACGACGGTTGGGAATTCAAGAAAGGTCCGTTTTCCAAAGAAGCATTGCAGGCTGTACAGAAATGGAATTCAGACTGGCAGGAGGTATCCATCCCACATACTTGGAATGCGGACGACATGCAAAAGCAAGCCTCCGCTTTCTATGAAGGTGTAGGCTACTACCGCAAAAAGTTCACTTTCCCCGAAAATATGGAAGGCAAGCGCATCTTTCTACGCTTCGAGGGTGTAGGTTCTTATGCCGAAGTATATATCAATGGCTATCTGGCAGGAACACACAAAGGAGCTTACTCTGCTTTTGCATGCGAGATCAGCTCGCAAGTGAAGTTCGGCAAGGAAAACGAAATTATAGTAAAGGCAGACAACTCCTCACGTCCCGATGTTATCCCCACGAACCACATTTTGTTCGGTGTTTATGGAGGTATTTACCGCCCCGTATGGCTGGTTGTTACGGAAACTTGCAGCATCATTGCAAACGACTGTGCCTCTCCGGGAGTGTATATTACCCAGAAAAATGTTTCAAAGAAGTCAGCCGAAGTGACTGTAAAAGTAAAGGTGGACAATGCAACCTTAGCTCCCGTCCCACTGGAACTAGAGAATGCAATATACGACAGGAACGGCAAATTGGTAAAGAAACATAGTCAGAATTTCGAACTTACTCCACAAGGAGTGCAGAACTATTCCTCACACTTCCGACTGAACAATCCCCATTTATGGCAAGGACGTGAAGACCCATATCTGTATAAAGTCGTTTCACGTCTCATGCAGAATGGACGCGTGATAGACGAAGTTATTCAGCCCCTGGGGATTCGCAAGTATGAAATCGTGGCAGGCAAAGGATTCTTCCTCAATGGAAAGAAGTACCCCATGCTGGGGGTAACCCGCCATCAAGACTGGTGGGGACTGGGTAGCGCCCTCACCAATAAAGAGCACGACTTCGACTTGGCACAAATTATGGATATCGGTGCAACCACCGTCCGCTTTGCACATTACCAACAGTCTGACTACATCTATTCACGCTGCGACACGCTGGGATTGGTAATCTGGGCAGAGATCCCCTTCGTGAACCGAGTCAGCGGCCAAGAGTGGGACAATGCCCACCAACAGATGCGCGAACTTATCCGTCAAAGCTTTAATCATCCCTCCATCTATGTATGGGGAATCCATAATGAAGTGTATCATCCGCATGGTTATACCGCCGCACTTACCCAATCCATTCACGACCTCTGCAAACTGGAGGATCCAGACCGCTACACGGTTGCGGTAAATGGTTACGGTCATGCCGATCACCCCGTCAACCAAAATGCGGATATACAGGGCATGAACCGTTACTTCGGTTGGTACGAACGGAAGATACAAGACATCAAGCCATGGATAGAGAAAATGGAAAAAGAATATCCTTGGCAACGCCTGATGCTGACAGAATATGGTGCAGACGCCAATATAGAACATCAGACGGAAATATTGGGCGATGCACTGAATTGGACAAGCCCTTTCTATCCGGAAACATTTCAAACTAAAACCCACGAATACCAATGGAGCATCATTGCCCAACATCCTTACATCATCGCTTCTTACCTGTGGAATATGTTCGACTTTGCAGTGCCAACATCAAAAAGAGGCAGTGTAGACGCCCGCAACATGAAAGGCATGATGACTTTCGATCGTAGAATCAAGAAAGATTCTTATTTCTGGTATAAGGCGAACTGGAGCAAGGAACCG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,784 total letters Query= CMR200019.107 Length=2142 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3124 0.0 1_02C_C5_CMR200019.107_T7term.seq 580 1e-168 R E V A S F N D G W E F K K G P F S K E A L Q A V Q K W N S Query_1 202 CGTGAGGTAGCATCCTTCAACGACGGTTGGGAATTCAAGAAAGGTCCGTTTTCCAAAGAAGCATTGCAGGCTGTACAGAAATGGAATTCA 291 0 1 .......................................................................................... 90 D W Q E V S I P H T W N A D D M Q K Q A S A F Y E G V G Y Y Query_1 292 GACTGGCAGGAGGTATCCATCCCACATACTTGGAATGCGGACGACATGCAAAAGCAAGCCTCCGCTTTCTATGAAGGTGTAGGCTACTAC 381 0 91 .......................................................................................... 180 0 320 ............ 309 R K K F T F P E N M E G K R I F L R F E G V G S Y A E V Y I Query_1 382 CGCAAAAAGTTCACTTTCCCCGAAAATATGGAAGGCAAGCGCATCTTTCTACGCTTCGAGGGTGTAGGTTCTTATGCCGAAGTATATATC 471 0 181 .......................................................................................... 270 N G Y L A G T H K G A Y S A F A C E I S S Q V K F G K E N E Query_1 472 AATGGCTATCTGGCAGGAACACACAAAGGAGCTTACTCTGCTTTTGCATGCGAGATCAGCTCGCAAGTGAAGTTCGGCAAGGAAAACGAA 561 0 271 .......................................................................................... 360 0 146 ............ 135 I I V K A D N S S R P D V I P T N H I L F G V Y G G I Y R P Query_1 562 ATTATAGTAAAGGCAGACAACTCCTCACGTCCCGATGTTATCCCCACGAACCACATTTTGTTCGGTGTTTATGGAGGTATTTACCGCCCC 651 0 361 .......................................................................................... 450 V W L V V T E T C S I I A N D C A S P G V Y I T Q K N V S K Query_1 652 GTATGGCTGGTTGTTACGGAAACTTGCAGCATCATTGCAAACGACTGTGCCTCTCCGGGAGTGTATATTACCCAGAAAAATGTTTCAAAG 741 0 451 .......................................................................................... 540 0 1552 ..G........... 1565 1 557 ..G........... 544 K S A E V T V K V K V D N A T L A P V P L E L E N A I Y D R Query_1 742 AAGTCAGCCGAAGTGACTGTAAAAGTAAAGGTGGACAATGCAACCTTAGCTCCCGTCCCACTGGAACTAGAGAATGCAATATACGACAGG 831 0 541 .......................................................................................... 630 N G K L V K K H S Q N F E L T P Q G V Q N Y S S H F R L N N Query_1 832 AACGGCAAATTGGTAAAGAAACATAGTCAGAATTTCGAACTTACTCCACAAGGAGTGCAGAACTATTCCTCACACTTCCGACTGAACAAT 921 0 631 .......................................................................................... 720 P H L W Q G R E D P Y L Y K V V S R L M Q N G R V I D E V I Query_1 922 CCCCATTTATGGCAAGGACGTGAAGACCCATATCTGTATAAAGTCGTTTCACGTCTCATGCAGAATGGACGCGTGATAGACGAAGTTATT 1011 0 721 .......................................................................................... 810 Q P L G I R K Y E I V A G K G F F L N G K K Y P M L G V T R Query_1 1012 CAGCCCCTGGGGATTCGCAAGTATGAAATCGTGGCAGGCAAAGGATTCTTCCTCAATGGAAAGAAGTACCCCATGCTGGGGGTAACCCGC 1101 0 811 .......................................................................................... 900 H Q D W W G L G S A L T N K E H D F D L A Q I M D I G A T T Query_1 1102 CATCAAGACTGGTGGGGACTGGGTAGCGCCCTCACCAATAAAGAGCACGACTTCGACTTGGCACAAATTATGGATATCGGTGCAACCACC 1191 0 901 .......................................................................................... 990 V R F A H Y Q Q S D Y I Y S R C D T L G L V I W A E I P F V Query_1 1192 GTCCGCTTTGCACATTACCAACAGTCTGACTACATCTATTCACGCTGCGACACGCTGGGATTGGTAATCTGGGCAGAGATCCCCTTCGTG 1281 0 991 .......................................................................................... 1080 N R V S G Q E W D N A H Q Q M R E L I R Q S F N H P S I Y V Query_1 1282 AACCGAGTCAGCGGCCAAGAGTGGGACAATGCCCACCAACAGATGCGCGAACTTATCCGTCAAAGCTTTAATCATCCCTCCATCTATGTA 1371 0 1081 .......................................................................................... 1170 W G I H N E V Y H P H G Y T A A L T Q S I H D L C K L E D P Query_1 1372 TGGGGAATCCATAATGAAGTGTATCATCCGCATGGTTATACCGCCGCACTTACCCAATCCATTCACGACCTCTGCAAACTGGAGGATCCA 1461 0 1171 .......................................................................................... 1260 D R Y T V A V N G Y G H A D H P V N Q N A D I Q G M N R Y F Query_1 1462 GACCGCTACACGGTTGCGGTAAATGGTTACGGTCATGCCGATCACCCCGTCAACCAAAATGCGGATATACAGGGCATGAACCGTTACTTC 1551 0 1261 .......................................................................................... 1350 G W Y E R K I Q D I K P W I E K M E K E Y P W Q R L M L T Q Query_1 1552 GGTTGGTACGAACGGAAGATACAAGACATCAAGCCATGGATAGAGAAAATGGAAAAAGAATATCCTTGGCAACGCCTGATGCTGACACAG 1641 0 1351 .......................................................................................G.A 1440 1 692 ........................ 669 Y G A D A N I E H Q T E I L G D A L N W T S P F Y P E T F Q Query_1 1642 TATGGTGCAGACGCCAATATAGAACATCAGACGGAAATATTGGGCGATGCACTGAATTGGACAAGCCCTTTCTATCCGGAAACATTTCAA 1731 0 1441 .......................................................................................... 1530 1 668 .......................................................................................... 579 T K T H E Y Q W S I I A Q H P Y I I A S Y L W N M F D F A V Query_1 1732 ACTAAAACCCACGAATACCAATGGAGCATCATTGCCCAACATCCTTACATCATCGCTTCTTACCTGTGGAATATGTTCGACTTTGCAGTG 1821 0 1531 .......................................................................................... 1620 0 475 ..C........... 488 1 578 .......................................................................................... 489 P T S K R G S V D A R N M K G M M T F D R R I K K D S Y F W Query_1 1822 CCAACATCAAAAAGAGGCAGTGTAGACGCCCGCAACATGAAAGGCATGATGACTTTCGATCGTAGAATCAAGAAAGATTCTTATTTCTGG 1911 0 1621 .......................................................................................... 1710 1 488 .......................................................................................... 399 Y K A N W S K E P * H W K W I T D P N S S S V D K L A A A L Query_1 1912 TATAAGGCGAACTGGAGCAAGGAACCGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTC 2001 0 1711 ........................... 1737 1 398 ........................... 372 1 66 .....................--..... 41 E H H H H H H * D P A A N K A R K E Query_1 2002 GAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAA 2055 1 45 .....................N...A... 17 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5816736 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 2,784 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200020.106 (CMR200020.21-1021.P(1021).E480A.pMCSG68)
QTVQPEWQSQYAIGKNKLAPHTYIWPYANADAAGEGNYEQSPYYMSLNGKWKFHWVKNPDHRPKDFYKPSFYTGGWADIDVPGNWERQGYGTAIYVNETYEFDDPLFNFKKNPPLVPYAENEVGSYRRTFTVPADWKDRRIVICCEGVISFYYIWVNGHMLGYNQGSKTPAEWDITDYVQAGENIVALEVYRWSAGSYLECQDMWRLSGIERDVYLYSTPRQFIADYKVTSSLDKKEYKEGFFGLDVTVEGSATGVSTLAYSLEDTAGKTILQNEIPIKNRGLSNSIIFDEQSLPGVKRWSAEHPNLYTLILTLKDDAGHITHLTGCKVGFRTSEIKDGRFCINGVPILIKGANRHEHSQLGRTVSKELMEEDIRLMKQHNLNTVRNSHYPTHPYWYQLCDRYGLYVIDEANIESHGMGYGPASLAKDSTWLPAHMDRTQRMYERAKNHPGIVIWSLGNEAGNGINFERTYDWMKSVETTRPIQYERAEQNYNTDIYCRMYRSVEELLAYARQTEPKVYRPFIMTEYLHAMGNSGGGLKEYMDVFETEPIVQGGCIWDWVDQSFREIDADGRWYWTYGGDYGPKNVPSFGNFCCNGLVNAVREPHPHLLEVKKAYQYIKTTLIDKSNLTLSIKNWYDFTNLNAYTLHWQVVGDHGKVITDGTRRVDAAPHATVEVTLGAVRLPSDVREAYLNLSWTSVDAAPFINTDYEVAYDQFVLSGNKNYRAPETKLSGKVEMNIDPATGALHSYIYNGKELLVSPVTLSLYRPVTDNDNREKVGGAKAWKKAGLDQLTQRALTVKTSTRSGRAEVELLNVRGEKVGTASFIYTLKKNGELDIQTHFVPDTTVITSLARVGLTFEMPDAYNQVSYLGRGEHETYMDRNQSGKIGIYHTDVESMFHYYVRPQATGNRTDVRWMQLADEAGEGLAFRSDASFQFSVIPFTDENLDAATHINQLRRDGVVTVHLDAAQAGVGTATCGPGVLPQYRVPVQDYIFRFTIRPLK
CAGACTGTACAGCCGGAATGGCAGAGCCAGTATGCTATCGGAAAAAACAAACTGGCACCACATACTTATATATGGCCTTATGCGAATGCTGATGCTGCGGGTGAAGGGAATTATGAGCAATCACCTTATTATATGAGTCTGAACGGTAAGTGGAAATTCCACTGGGTGAAGAATCCCGATCATCGTCCGAAAGACTTTTATAAACCTTCGTTCTATACGGGCGGATGGGCAGATATCGATGTACCCGGTAACTGGGAGCGTCAGGGATATGGTACGGCTATTTATGTGAACGAAACTTATGAATTTGATGATCCTTTGTTCAACTTCAAAAAGAATCCTCCGCTGGTACCTTATGCAGAGAACGAAGTAGGTTCTTATCGCCGTACGTTCACGGTTCCTGCTGACTGGAAAGACCGTCGCATCGTGATTTGCTGTGAAGGTGTGATCTCTTTCTATTATATCTGGGTGAACGGACATATGCTGGGTTATAACCAGGGTTCAAAGACTCCTGCCGAATGGGATATCACGGACTATGTACAGGCAGGAGAAAATATCGTGGCACTTGAAGTATATCGCTGGAGTGCAGGTTCCTATCTGGAGTGTCAGGATATGTGGCGGTTGAGTGGCATTGAACGGGATGTCTATCTTTACAGTACTCCACGCCAGTTTATAGCTGATTATAAAGTGACTTCTTCTTTGGATAAGAAAGAGTATAAGGAAGGATTTTTCGGCCTTGATGTAACAGTTGAAGGAAGTGCGACTGGTGTGTCTACTTTGGCATATAGTCTGGAAGATACTGCCGGAAAGACAATATTACAAAATGAAATACCGATCAAGAACCGTGGTTTGAGTAATTCTATTATCTTTGACGAACAGTCTCTTCCAGGTGTAAAACGTTGGAGTGCAGAACATCCGAATCTTTATACGTTAATATTGACGTTGAAAGATGATGCAGGACATATAACACACCTGACCGGTTGCAAAGTTGGTTTCCGTACTTCTGAAATCAAGGATGGCCGTTTTTGTATCAACGGGGTACCTATATTGATAAAAGGTGCTAACCGGCATGAACATTCACAGTTGGGACGTACTGTCAGCAAAGAACTGATGGAAGAAGACATTCGCTTGATGAAGCAACATAACCTGAATACTGTGCGCAACTCGCATTATCCGACACATCCGTACTGGTATCAACTCTGCGACCGTTACGGCCTTTATGTGATAGATGAAGCAAACATCGAATCGCATGGTATGGGGTATGGCCCGGCTTCATTGGCAAAGGATAGCACTTGGCTGCCTGCACACATGGACCGTACGCAACGTATGTACGAACGTGCCAAGAACCATCCGGGGATCGTGATCTGGTCGTTGGGCAACGAAGCGGGTAACGGAATCAATTTTGAACGGACATATGATTGGATGAAGTCCGTTGAAACCACCCGCCCTATACAATACGAGCGTGCCGAACAGAATTATAATACGGATATCTACTGCCGTATGTACCGCAGTGTGGAAGAATTGCTGGCCTATGCCCGCCAGACAGAACCGAAAGTGTATCGCCCTTTCATCATGACTGAATATCTGCATGCTATGGGCAATAGCGGAGGCGGATTGAAAGAATACATGGATGTTTTCGAAACAGAACCTATAGTGCAGGGCGGTTGTATCTGGGATTGGGTAGATCAGTCTTTCCGTGAAATAGATGCCGATGGTCGTTGGTATTGGACTTACGGTGGTGATTATGGTCCGAAGAATGTACCCAGTTTCGGGAACTTTTGCTGCAATGGTCTGGTGAATGCTGTCCGCGAACCTCATCCGCATTTGCTGGAAGTGAAGAAAGCATATCAATATATAAAGACTACATTGATTGATAAGAGCAACTTGACTTTAAGTATAAAGAATTGGTATGATTTTACGAATCTCAATGCTTATACCTTACACTGGCAGGTGGTAGGTGATCATGGGAAAGTGATTACTGATGGTACTCGTCGGGTAGATGCCGCTCCGCACGCCACCGTTGAAGTTACGCTGGGTGCTGTACGTTTACCTTCTGATGTTCGTGAAGCTTATCTGAACTTAAGTTGGACGTCTGTGGATGCTGCTCCGTTTATTAATACGGATTACGAGGTAGCCTATGACCAATTTGTTCTTTCGGGTAATAAGAACTACCGGGCTCCGGAAACTAAACTTTCGGGTAAGGTGGAAATGAATATAGATCCGGCAACAGGGGCGCTTCACTCTTATATTTATAATGGGAAAGAATTACTGGTTTCCCCCGTTACATTGAGCCTCTATCGTCCGGTGACGGATAATGATAACCGTGAAAAAGTAGGAGGAGCCAAAGCTTGGAAGAAAGCTGGGTTGGATCAGTTAACACAGCGTGCCCTTACCGTGAAGACTTCTACCCGCAGTGGACGTGCCGAAGTGGAATTATTAAATGTCCGTGGTGAAAAAGTGGGAACCGCTTCTTTCATCTATACACTAAAGAAAAATGGCGAATTGGATATACAGACACACTTTGTGCCGGATACTACCGTTATCACTTCACTGGCGCGTGTAGGGCTTACTTTTGAAATGCCCGATGCTTACAATCAGGTGTCTTATCTGGGGCGTGGAGAGCATGAAACTTATATGGATCGTAATCAATCCGGTAAAATAGGTATTTATCACACCGATGTGGAGAGTATGTTCCACTATTATGTTCGTCCGCAGGCAACGGGTAACCGTACGGATGTCCGTTGGATGCAACTTGCTGATGAGGCAGGTGAGGGGCTGGCATTCCGTTCCGATGCCTCGTTTCAGTTTAGTGTGATTCCTTTTACGGATGAGAATCTGGATGCGGCAACGCATATCAATCAATTACGGCGCGATGGAGTGGTAACTGTTCATCTGGATGCCGCACAGGCAGGAGTCGGTACGGCTACCTGTGGTCCTGGAGTATTGCCGCAGTATCGTGTACCTGTGCAGGATTACATATTCCGGTTTACAATCCGTCCTTTGAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,100 total letters Query= CMR200020.106 Length=3408 Score E Sequences producing significant alignments: (Bits) Value 0_wt 5407 0.0 1_02D_D5_CMR200020.106_T7term.seq 1734 0.0 2_09H_D5_CMR200020.106_T7S.seq 1508 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 57 ATTCCCCTCTAGAAATAATTTT-GTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGT 145 2 24 .....A...C.N.T-......CC.........NCT.............C..................A...................... 112 W S H P Q F E K G T E N L Y F Q S N A Q T V Q P E W Q S Q Y Query_1 146 GGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAGACTGTACAGCCGGAATGGCAGAGCCAGTATG 235 0 1 .................................. 34 2 113 ..C..............TN...............................................N....................... 202 A I G K N K L A P H T Y I W P Y A N A D A A G E G N Y E Q S Query_1 236 CTATCGGAAAAAACAAACTGGCACCACATACTTATATATGGCCTTATGCGAATGCTGATGCTGCGGGTGAAGGGAATTATGAGCAATCAC 325 0 35 .......................................................................................... 124 2 203 .......................................................................................... 292 P Y Y M S L N G K W K F H W V K N P D H R P K D F Y K P S F Query_1 326 CTTATTATATGAGTCTGAACGGTAAGTGGAAATTCCACTGGGTGAAGAATCCCGATCATCGTCCGAAAGACTTTTATAAACCTTCGTTCT 415 0 125 .......................................................................................... 214 2 293 .......................................................................................... 382 Y T G G W A D I D V P G N W E R Q G Y G T A I Y V N E T Y E Query_1 416 ATACGGGCGGATGGGCAGATATCGATGTACCCGGTAACTGGGAGCGTCAGGGATATGGTACGGCTATTTATGTGAACGAAACTTATGAAT 505 0 215 .......................................................................................... 304 2 383 ..................G.............................N......................................... 472 F D D P L F N F K K N P P L V P Y A E N E V G S Y R R T F T Query_1 506 TTGATGATCCTTTGTTCAACTTCAAAAAGAATCCTCCGCTGGTACCTTATGCAGAGAACGAAGTAGGTTCTTATCGCCGTACGTTCACGG 595 0 305 .......................................................................................... 394 2 473 .....................................................N.................................... 562 V P A D W K D R R I V I C C E G V I S F Y Y I W V N G H M L Query_1 596 TTCCTGCTGACTGGAAAGACCGTCGCATCGTGATTTGCTGTGAAGGTGTGATCTCTTTCTATTATATCTGGGTGAACGGACATATGCTGG 685 0 395 .......................................................................................... 484 0 1401 .............. 1414 2 563 ......................N.N....NN..................................................N........ 652 G Y N Q G S K T P A E W D I T D Y V Q A G E N I V A L E V Y Query_1 686 GTTATAACCAGGGTTCAAAGACTCCTGCCGAATGGGATATCACGGACTATGTACAGGCAGGAGAAAATATCGTGGCACTTGAAGTATATC 775 0 485 .......................................................................................... 574 0 2901 ........... 2911 1 235 ........... 225 2 653 ..............................................................N........................... 742 R W S A G S Y L E C Q D M W R L S G I E R D V Y L Y S T P R Query_1 776 GCTGGAGTGCAGGTTCCTATCTGGAGTGTCAGGATATGTGGCGGTTGAGTGGCATTGAACGGGATGTCTATCTTTACAGTACTCCACGCC 865 0 575 .......................................................................................... 664 2 743 ...........T.............................N........................CNN........N.-.......... 831 Q F I A D Y K V T S S L D K K E Y K E G F F G L D V T V E G Query_1 866 AGTTTATAGCTGATTATAAAGTGACTT-CTTCTTTGGATAAGAAAGAGTATAAGGAAGGATTTTTCGGCCTTGATGTAACAGTTGAAGGA 954 0 665 ...........................-.............................................................. 753 2 832 ..-........................N......NN...........NATN.C.T..NN.......N...........C...N....N.. 920 S A T G V S T L A Y S L E D T A G K T I L Q N E I P I K N R Query_1 955 AGTGCGACTGGTGTGTCTACTTTGGCATATAGTCTGGAAGATACTGCCGGAAAGACAATATTACAAAATGAAATACCGATCAAGAACCGT 1044 0 754 .......................................................................................... 843 2 921 ..A........C..........N...N...N...........................C.-.N....TGN..T..TGN....TN...... 1009 G L S N S I I F D E Q S L P G V K R W S A E H P N L Y T L Query_1 1045 GG-TTTG-AGTAATTCTATTATCTTTGACGAACAGTCTCTTCCAGGTGTAAAACGTTGGAGTGCAGAACATCCGAATCTTTATACGTTAA 1132 0 844 ..-....-.................................................................................. 931 2 1010 ..C....N.CNN........ 1029 I L T L K D D A G H I T H L T G C K V G F R T S E I K D G R Query_1 1133 TATTGACGTTGAAAGATGATGCAGGACATATAACACACCTGACCGGTTGCAAAGTTGGTTTCCGTACTTCTGAAATCAAGGATGGCCGTT 1222 0 932 .......................................................................................... 1021 F C I N G V P I L I K G A N R H E H S Q L G R T V S K E L M Query_1 1223 TTTGTATCAACGGGGTACCTATATTGATAAAAGGTGCTAACCGGCATGAACATTCACAGTTGGGACGTACTGTCAGCAAAGAACTGATGG 1312 0 1022 .......................................................................................... 1111 E E D I R L M K Q H N L N T V R N S H Y P T H P Y W Y Q L C Query_1 1313 AAGAAGACATTCGCTTGATGAAGCAACATAACCTGAATACTGTGCGCAACTCGCATTATCCGACACATCCGTACTGGTATCAACTCTGCG 1402 0 1112 .......................................................................................... 1201 D R Y G L Y V I D E A N I E S H G M G Y G P A S L A K D S T Query_1 1403 ACCGTTACGGCCTTTATGTGATAGATGAAGCAAACATCGAATCGCATGGTATGGGGTATGGCCCGGCTTCATTGGCAAAGGATAGCACTT 1492 0 1202 .......................................................................................... 1291 W L P A H M D R T Q R M Y E R A K N H P G I V I W S L G N A Query_1 1493 GGCTGCCTGCACACATGGACCGTACGCAACGTATGTACGAACGTGCCAAGAACCATCCGGGGATCGTGATCTGGTCGTTGGGCAACGCTG 1582 0 1292 .......................................................................................AA. 1381 A G N G I N F E R T Y D W M K S V E T T R P I Q Y E R A E Q Query_1 1583 CGGGTAACGGAATCAATTTTGAACGGACATATGATTGGATGAAGTCCGTTGAAACCACCCGCCCTATACAATACGAGCGTGCCGAACAGA 1672 0 1382 .......................................................................................... 1471 0 467 .............. 480 2 635 .........N.... 648 N Y N T D I Y C R M Y R S V E E L L A Y A R Q T E P K V Y R Query_1 1673 ATTATAATACGGATATCTACTGCCGTATGTACCGCAGTGTGGAAGAATTGCTGGCCTATGCCCGCCAGACAGAACCGAAAGTGTATCGCC 1762 0 1472 .......................................................................................... 1561 P F I M T E Y L H A M G N S G G G L K E Y M D V F E T E P I Query_1 1763 CTTTCATCATGACTGAATATCTGCATGCTATGGGCAATAGCGGAGGCGGATTGAAAGAATACATGGATGTTTTCGAAACAGAACCTATAG 1852 0 1562 .......................................................................................... 1651 V Q G G C I W D W V D Q S F R E I D A D G R W Y W T Y G G D Query_1 1853 TGCAGGGCGGTTGTATCTGGGATTGGGTAGATCAGTCTTTCCGTGAAATAGATGCCGATGGTCGTTGGTATTGGACTTACGGTGGTGATT 1942 0 1652 .......................................................................................... 1741 Y G P K N V P S F G N F C C N G L V N A V R E P H P H L L E Query_1 1943 ATGGTCCGAAGAATGTACCCAGTTTCGGGAACTTTTGCTGCAATGGTCTGGTGAATGCTGTCCGCGAACCTCATCCGCATTTGCTGGAAG 2032 0 1742 .......................................................................................... 1831 2 115 ...........G.. 128 V K K A Y Q Y I K T T L I D K S N L T L S I K N W Y D F T N Query_1 2033 TGAAGAAAGCATATCAATATATAAAGACTACATTGATTGATAAGAGCAACTTGACTTTAAGTATAAAGAATTGGTATGATTTTACGAATC 2122 0 1832 .......................................................................................... 1921 0 2860 ........... 2850 1 276 ........... 286 L N A Y T L H W Q V V G D H G K V I T D G T R R V D A A P H Query_1 2123 TCAATGCTTATACCTTACACTGGCAGGTGGTAGGTGATCATGGGAAAGTGATTACTGATGGTACTCGTCGGGTAGATGCCGCTCCGCACG 2212 0 1922 .......................................................................................... 2011 A T V E V T L G A V R L P S D V R E A Y L N L S W T S V D A Query_1 2213 CCACCGTTGAAGTTACGCTGGGTGCTGTACGTTTACCTTCTGATGTTCGTGAAGCTTATCTGAACTTAAGTTGGACGTCTGTGGATGCTG 2302 0 2012 .......................................................................................... 2101 1 1047 ..N.... 1041 A P F I N T D Y E V A Y D Q F V L S G N K N Y R A P E T Query_1 2303 C-TCCG-TTTATTAA-TACGGATTACGA-GGTAGCCTATGACCAATTTGTTCTTTCGGGTAA-TAAGAAC-TACCGGGCTCCGGAAACTA 2386 0 2102 .-....-........-............-.................................-.......-................... 2185 1 1040 .N...NN........A............A..................-..............A.......N...............-... 953 K L S G K V E M N I D P A T G A L H S Y I Y N G K E L L V Query_1 2387 AACTTTCGGGTAA-GGTGGAAATGAATATAGATCCGGCAACAGGGGCGCTTCACTCTTATATTTATAATGGGAAA-GAATTA-CTGGTTT 2473 0 2186 .............-.............................................................-......-....... 2272 1 952 .....-.......A.............................................................A......A....... 864 S P V T L S L Y R P V T D N D N R E K V G G A K A W K K A G Query_1 2474 CCCCCGTTACATTGAGCCTCTATCGTCCGGTGACGGATAATGATAACCGTGAAAAAGTAGGAGGAGCCAAAGCTTGGAAGAAAGCTGGGT 2563 0 2273 .......................................................................................... 2362 1 863 .............................................N............................................ 774 L D Q L T Q R A L T V K T S T R S G R A E V E L L N V R G E Query_1 2564 TGGATCAGTTAACACAGCGTGCCCTTACCGTGAAGACTTCTACCCGCAGTGGACGTGCCGAAGTGGAATTATTAAATGTCCGTGGTGAAA 2653 0 2363 .......................................................................................... 2452 1 773 ..........................N............................................................... 684 K V G T A S F I Y T L K K N G E L D I Q T H F V P D T T V I Query_1 2654 AAGTGGGAACCGCTTCTTTCATCTATACACTAAAGAAAAATGGCGAATTGGATATACAGACACACTTTGTGCCGGATACTACCGTTATCA 2743 0 2453 .......................................................................................... 2542 1 683 .......................................................................................... 594 T S L A R V G L T F E M P D A Y N Q V S Y L G R G E H E T Y Query_1 2744 CTTCACTGGCGCGTGTAGGGCTTACTTTTGAAATGCCCGATGCTTACAATCAGGTGTCTTATCTGGGGCGTGGAGAGCATGAAACTTATA 2833 0 2543 .......................................................................................... 2632 1 593 .......................................................................................... 504 M D R N Q S G K I G I Y H T D V E S M F H Y Y V R P Q A T G Query_1 2834 TGGATCGTAATCAATCCGGTAAAATAGGTATTTATCACACCGATGTGGAGAGTATGTTCCACTATTATGTTCGTCCGCAGGCAACGGGTA 2923 0 2633 .......................................................................................... 2722 1 503 .......................................................................................... 414 N R T D V R W M Q L A D E A G E G L A F R S D A S F Q F S V Query_1 2924 ACCGTACGGATGTCCGTTGGATGCAACTTGCTGATGAGGCAGGTGAGGGGCTGGCATTCCGTTCCGATGCCTCGTTTCAGTTTAGTGTGA 3013 0 2723 .......................................................................................... 2812 1 413 .......................................................................................... 324 I P F T D E N L D A A T H I N Q L R R D G V V T V H L D A A Query_1 3014 TTCCTTTTACGGATGAGAATCTGGATGCGGCAACGCATATCAATCAATTACGGCGCGATGGAGTGGTAACTGTTCATCTGGATGCCGCAC 3103 0 2813 .......................................................................................... 2902 0 537 .. 538 1 323 .......................................................................................... 234 Q A G V G T A T C G P G V L P Q Y R V P V Q D Y I F R F T I Query_1 3104 AGGCAGGAGTCGGTACGGCTACCTGTGGTCCTGGAGTATTGCCGCAGTATCGTGTACCTGTGCAGGATTACATATTCCGGTTTACAATCC 3193 0 2903 .......................................................................................... 2992 0 539 ......... 547 1 233 .......................................................................................... 144 R P L K * H W K W I T D P N S S S V D K L A A A L E H H H H Query_1 3194 GTCCTTTGAAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccacc 3283 0 2993 ........... 3003 1 143 .............................................................NN........................... 54 H H * D P A A N K A R K E Query_1 3284 accacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAG 3322 1 53 ..........................N...N...A.N.. 15 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 17105940 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 5,100 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200020.107 (CMR200020.21-1021.P(1021).E546A.pMCSG68)
QTVQPEWQSQYAIGKNKLAPHTYIWPYANADAAGEGNYEQSPYYMSLNGKWKFHWVKNPDHRPKDFYKPSFYTGGWADIDVPGNWERQGYGTAIYVNETYEFDDPLFNFKKNPPLVPYAENEVGSYRRTFTVPADWKDRRIVICCEGVISFYYIWVNGHMLGYNQGSKTPAEWDITDYVQAGENIVALEVYRWSAGSYLECQDMWRLSGIERDVYLYSTPRQFIADYKVTSSLDKKEYKEGFFGLDVTVEGSATGVSTLAYSLEDTAGKTILQNEIPIKNRGLSNSIIFDEQSLPGVKRWSAEHPNLYTLILTLKDDAGHITHLTGCKVGFRTSEIKDGRFCINGVPILIKGANRHEHSQLGRTVSKELMEEDIRLMKQHNLNTVRNSHYPTHPYWYQLCDRYGLYVIDEANIESHGMGYGPASLAKDSTWLPAHMDRTQRMYERAKNHPGIVIWSLGNEAGNGINFERTYDWMKSVETTRPIQYERAEQNYNTDIYCRMYRSVEELLAYARQTEPKVYRPFIMTEYLHAMGNSGGGLKEYMDVFETEPIVQGGCIWDWVDQSFREIDADGRWYWTYGGDYGPKNVPSFGNFCCNGLVNAVREPHPHLLEVKKAYQYIKTTLIDKSNLTLSIKNWYDFTNLNAYTLHWQVVGDHGKVITDGTRRVDAAPHATVEVTLGAVRLPSDVREAYLNLSWTSVDAAPFINTDYEVAYDQFVLSGNKNYRAPETKLSGKVEMNIDPATGALHSYIYNGKELLVSPVTLSLYRPVTDNDNREKVGGAKAWKKAGLDQLTQRALTVKTSTRSGRAEVELLNVRGEKVGTASFIYTLKKNGELDIQTHFVPDTTVITSLARVGLTFEMPDAYNQVSYLGRGEHETYMDRNQSGKIGIYHTDVESMFHYYVRPQATGNRTDVRWMQLADEAGEGLAFRSDASFQFSVIPFTDENLDAATHINQLRRDGVVTVHLDAAQAGVGTATCGPGVLPQYRVPVQDYIFRFTIRPLK
CAGACTGTACAGCCGGAATGGCAGAGCCAGTATGCTATCGGAAAAAACAAACTGGCACCACATACTTATATATGGCCTTATGCGAATGCTGATGCTGCGGGTGAAGGGAATTATGAGCAATCACCTTATTATATGAGTCTGAACGGTAAGTGGAAATTCCACTGGGTGAAGAATCCCGATCATCGTCCGAAAGACTTTTATAAACCTTCGTTCTATACGGGCGGATGGGCAGATATCGATGTACCCGGTAACTGGGAGCGTCAGGGATATGGTACGGCTATTTATGTGAACGAAACTTATGAATTTGATGATCCTTTGTTCAACTTCAAAAAGAATCCTCCGCTGGTACCTTATGCAGAGAACGAAGTAGGTTCTTATCGCCGTACGTTCACGGTTCCTGCTGACTGGAAAGACCGTCGCATCGTGATTTGCTGTGAAGGTGTGATCTCTTTCTATTATATCTGGGTGAACGGACATATGCTGGGTTATAACCAGGGTTCAAAGACTCCTGCCGAATGGGATATCACGGACTATGTACAGGCAGGAGAAAATATCGTGGCACTTGAAGTATATCGCTGGAGTGCAGGTTCCTATCTGGAGTGTCAGGATATGTGGCGGTTGAGTGGCATTGAACGGGATGTCTATCTTTACAGTACTCCACGCCAGTTTATAGCTGATTATAAAGTGACTTCTTCTTTGGATAAGAAAGAGTATAAGGAAGGATTTTTCGGCCTTGATGTAACAGTTGAAGGAAGTGCGACTGGTGTGTCTACTTTGGCATATAGTCTGGAAGATACTGCCGGAAAGACAATATTACAAAATGAAATACCGATCAAGAACCGTGGTTTGAGTAATTCTATTATCTTTGACGAACAGTCTCTTCCAGGTGTAAAACGTTGGAGTGCAGAACATCCGAATCTTTATACGTTAATATTGACGTTGAAAGATGATGCAGGACATATAACACACCTGACCGGTTGCAAAGTTGGTTTCCGTACTTCTGAAATCAAGGATGGCCGTTTTTGTATCAACGGGGTACCTATATTGATAAAAGGTGCTAACCGGCATGAACATTCACAGTTGGGACGTACTGTCAGCAAAGAACTGATGGAAGAAGACATTCGCTTGATGAAGCAACATAACCTGAATACTGTGCGCAACTCGCATTATCCGACACATCCGTACTGGTATCAACTCTGCGACCGTTACGGCCTTTATGTGATAGATGAAGCAAACATCGAATCGCATGGTATGGGGTATGGCCCGGCTTCATTGGCAAAGGATAGCACTTGGCTGCCTGCACACATGGACCGTACGCAACGTATGTACGAACGTGCCAAGAACCATCCGGGGATCGTGATCTGGTCGTTGGGCAACGAAGCGGGTAACGGAATCAATTTTGAACGGACATATGATTGGATGAAGTCCGTTGAAACCACCCGCCCTATACAATACGAGCGTGCCGAACAGAATTATAATACGGATATCTACTGCCGTATGTACCGCAGTGTGGAAGAATTGCTGGCCTATGCCCGCCAGACAGAACCGAAAGTGTATCGCCCTTTCATCATGACTGAATATCTGCATGCTATGGGCAATAGCGGAGGCGGATTGAAAGAATACATGGATGTTTTCGAAACAGAACCTATAGTGCAGGGCGGTTGTATCTGGGATTGGGTAGATCAGTCTTTCCGTGAAATAGATGCCGATGGTCGTTGGTATTGGACTTACGGTGGTGATTATGGTCCGAAGAATGTACCCAGTTTCGGGAACTTTTGCTGCAATGGTCTGGTGAATGCTGTCCGCGAACCTCATCCGCATTTGCTGGAAGTGAAGAAAGCATATCAATATATAAAGACTACATTGATTGATAAGAGCAACTTGACTTTAAGTATAAAGAATTGGTATGATTTTACGAATCTCAATGCTTATACCTTACACTGGCAGGTGGTAGGTGATCATGGGAAAGTGATTACTGATGGTACTCGTCGGGTAGATGCCGCTCCGCACGCCACCGTTGAAGTTACGCTGGGTGCTGTACGTTTACCTTCTGATGTTCGTGAAGCTTATCTGAACTTAAGTTGGACGTCTGTGGATGCTGCTCCGTTTATTAATACGGATTACGAGGTAGCCTATGACCAATTTGTTCTTTCGGGTAATAAGAACTACCGGGCTCCGGAAACTAAACTTTCGGGTAAGGTGGAAATGAATATAGATCCGGCAACAGGGGCGCTTCACTCTTATATTTATAATGGGAAAGAATTACTGGTTTCCCCCGTTACATTGAGCCTCTATCGTCCGGTGACGGATAATGATAACCGTGAAAAAGTAGGAGGAGCCAAAGCTTGGAAGAAAGCTGGGTTGGATCAGTTAACACAGCGTGCCCTTACCGTGAAGACTTCTACCCGCAGTGGACGTGCCGAAGTGGAATTATTAAATGTCCGTGGTGAAAAAGTGGGAACCGCTTCTTTCATCTATACACTAAAGAAAAATGGCGAATTGGATATACAGACACACTTTGTGCCGGATACTACCGTTATCACTTCACTGGCGCGTGTAGGGCTTACTTTTGAAATGCCCGATGCTTACAATCAGGTGTCTTATCTGGGGCGTGGAGAGCATGAAACTTATATGGATCGTAATCAATCCGGTAAAATAGGTATTTATCACACCGATGTGGAGAGTATGTTCCACTATTATGTTCGTCCGCAGGCAACGGGTAACCGTACGGATGTCCGTTGGATGCAACTTGCTGATGAGGCAGGTGAGGGGCTGGCATTCCGTTCCGATGCCTCGTTTCAGTTTAGTGTGATTCCTTTTACGGATGAGAATCTGGATGCGGCAACGCATATCAATCAATTACGGCGCGATGGAGTGGTAACTGTTCATCTGGATGCCGCACAGGCAGGAGTCGGTACGGCTACCTGTGGTCCTGGAGTATTGCCGCAGTATCGTGTACCTGTGCAGGATTACATATTCCGGTTTACAATCCGTCCTTTGAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,443 total letters Query= CMR200020.107 Length=3408 Score E Sequences producing significant alignments: (Bits) Value 0_wt 5407 0.0 1_02E_E5_CMR200020.107_T7term.seq 1855 0.0 2_05E_E5_CMR200020.107_T7S.seq 1784 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W S Query_1 61 CCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCT 150 2 12 ...............................................................N.......................... 101 H P Q F E K G T E N L Y F Q S N A Q T V Q P E W Q S Q Y A I Query_1 151 CATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAGACTGTACAGCCGGAATGGCAGAGCCAGTATGCTATC 240 0 1 ....................................... 39 2 102 .......................................................................................... 191 G K N K L A P H T Y I W P Y A N A D A A G E G N Y E Q S P Y Query_1 241 GGAAAAAACAAACTGGCACCACATACTTATATATGGCCTTATGCGAATGCTGATGCTGCGGGTGAAGGGAATTATGAGCAATCACCTTAT 330 0 40 .......................................................................................... 129 2 192 .......................................................................................... 281 Y M S L N G K W K F H W V K N P D H R P K D F Y K P S F Y T Query_1 331 TATATGAGTCTGAACGGTAAGTGGAAATTCCACTGGGTGAAGAATCCCGATCATCGTCCGAAAGACTTTTATAAACCTTCGTTCTATACG 420 0 130 .......................................................................................... 219 2 282 .......................................................................................... 371 G G W A D I D V P G N W E R Q G Y G T A I Y V N E T Y E F D Query_1 421 GGCGGATGGGCAGATATCGATGTACCCGGTAACTGGGAGCGTCAGGGATATGGTACGGCTATTTATGTGAACGAAACTTATGAATTTGAT 510 0 220 .......................................................................................... 309 2 372 .............G............................................................................ 461 D P L F N F K K N P P L V P Y A E N E V G S Y R R T F T V P Query_1 511 GATCCTTTGTTCAACTTCAAAAAGAATCCTCCGCTGGTACCTTATGCAGAGAACGAAGTAGGTTCTTATCGCCGTACGTTCACGGTTCCT 600 0 310 .......................................................................................... 399 2 462 .......................................................................................... 551 A D W K D R R I V I C C E G V I S F Y Y I W V N G H M L G Y Query_1 601 GCTGACTGGAAAGACCGTCGCATCGTGATTTGCTGTGAAGGTGTGATCTCTTTCTATTATATCTGGGTGAACGGACATATGCTGGGTTAT 690 0 400 .......................................................................................... 489 0 1401 .............. 1414 2 552 .......................................................................................... 641 N Q G S K T P A E W D I T D Y V Q A G E N I V A L E V Y R W Query_1 691 AACCAGGGTTCAAAGACTCCTGCCGAATGGGATATCACGGACTATGTACAGGCAGGAGAAAATATCGTGGCACTTGAAGTATATCGCTGG 780 0 490 .......................................................................................... 579 0 2901 ........... 2911 1 236 ........... 226 2 642 .......................................................................................... 731 S A G S Y L E C Q D M W R L S G I E R D V Y L Y S T P R Q F Query_1 781 AGTGCAGGTTCCTATCTGGAGTGTCAGGATATGTGGCGGTTGAGTGGCATTGAACGGGATGTCTATCTTTACAGTACTCCACGCCAGTTT 870 0 580 .......................................................................................... 669 2 732 .......................................................................................... 821 I A D Y K V T S S L D K K E Y K E G F F G L D V T V E G S Query_1 871 ATAGCTGATTATAAAGTGACTTCTTCTTTGGA-TAAGAAAGAGTATAAGGAAGGATTTTTCGGCCTTGATGTAACAGTTGAAGGAAGTGC 959 0 670 ................................-......................................................... 758 2 822 .............................N..T......................-................-................. 909 A T G V S T L A Y S L E D T A G K T I L Q N E I P I K N R G Query_1 960 GACTGGTGTGTCTACTTTGGCATATAGTCTGGAAGATACTGCCGGAAAGACAATATTACAAAATGAAATACCGATCAAGAACC-GTGGTT 1048 0 759 ...................................................................................-...... 847 2 910 ...CT..............A.N................................NC.........G..N.......N......G.N.C.. 999 L S N S I I F D E Q S L P G V K R W S A E H P N L Y T L I L Query_1 1049 TGAGTAATTCTATTATCTTTGACGAACAGTCTCTTCCAGGTGTAAAACGTTGGAGTGCAGAACATCCGAATCTTTATACGTTAATATTGA 1138 0 848 .......................................................................................... 937 2 1000 ....N.N.C......NN............NT...NT..-...GT.......-..NNCANN....NT.CN.NNN...-N....CN...... 1086 T L K D D A G H I T H L T G C K V G F R T S E I K D G R F Query_1 1139 CGTTG-AAAGATGATGCAGGACATA-TAACACACCTGACCGGT-TGCAAAGTTGGTTTC-CGTACTTCTGAAATCAAGGATGGCCGTTTT 1224 0 938 .....-...................-.................-...............-.............................. 1023 2 1087 ...NNA...........N.N..TN.T.T............C..C...-.......A...T..N..N....G..N....A....NNNNN.. 1175 C I N G V P I L I K G A N R H E H S Q L G R T V S K E L M E Query_1 1225 TGTATCAACGGGGTACCTATATTGATAAAAGGTGCTAACCGGCATGAACATTCACAGTTGGGACGTACTGTCAGCAAAGAACTGATGGAA 1314 0 1024 .......................................................................................... 1113 2 1176 ....A.N.. 1184 E D I R L M K Q H N L N T V R N S H Y P T H P Y W Y Q L C D Query_1 1315 GAAGACATTCGCTTGATGAAGCAACATAACCTGAATACTGTGCGCAACTCGCATTATCCGACACATCCGTACTGGTATCAACTCTGCGAC 1404 0 1114 .......................................................................................... 1203 R Y G L Y V I D E A N I E S H G M G Y G P A S L A K D S T W Query_1 1405 CGTTACGGCCTTTATGTGATAGATGAAGCAAACATCGAATCGCATGGTATGGGGTATGGCCCGGCTTCATTGGCAAAGGATAGCACTTGG 1494 0 1204 .......................................................................................... 1293 L P A H M D R T Q R M Y E R A K N H P G I V I W S L G N E A Query_1 1495 CTGCCTGCACACATGGACCGTACGCAACGTATGTACGAACGTGCCAAGAACCATCCGGGGATCGTGATCTGGTCGTTGGGCAACGAAGCG 1584 0 1294 .......................................................................................... 1383 G N G I N F E R T Y D W M K S V E T T R P I Q Y E R A E Q N Query_1 1585 GGTAACGGAATCAATTTTGAACGGACATATGATTGGATGAAGTCCGTTGAAACCACCCGCCCTATACAATACGAGCGTGCCGAACAGAAT 1674 0 1384 .......................................................................................... 1473 0 467 .............. 480 2 619 .............. 632 Y N T D I Y C R M Y R S V E E L L A Y A R Q T E P K V Y R P Query_1 1675 TATAATACGGATATCTACTGCCGTATGTACCGCAGTGTGGAAGAATTGCTGGCCTATGCCCGCCAGACAGAACCGAAAGTGTATCGCCCT 1764 0 1474 .......................................................................................... 1563 F I M T A Y L H A M G N S G G G L K E Y M D V F E T E P I V Query_1 1765 TTCATCATGACTGCTTATCTGCATGCTATGGGCAATAGCGGAGGCGGATTGAAAGAATACATGGATGTTTTCGAAACAGAACCTATAGTG 1854 0 1564 .............AA........................................................................... 1653 Q G G C I W D W V D Q S F R E I D A D G R W Y W T Y G G D Y Query_1 1855 CAGGGCGGTTGTATCTGGGATTGGGTAGATCAGTCTTTCCGTGAAATAGATGCCGATGGTCGTTGGTATTGGACTTACGGTGGTGATTAT 1944 0 1654 .......................................................................................... 1743 G P K N V P S F G N F C C N G L V N A V R E P H P H L L E V Query_1 1945 GGTCCGAAGAATGTACCCAGTTTCGGGAACTTTTGCTGCAATGGTCTGGTGAATGCTGTCCGCGAACCTCATCCGCATTTGCTGGAAGTG 2034 0 1744 .......................................................................................... 1833 K K A Y Q Y I K T T L I D K S N L T L S I K N W Y D F T N L Query_1 2035 AAGAAAGCATATCAATATATAAAGACTACATTGATTGATAAGAGCAACTTGACTTTAAGTATAAAGAATTGGTATGATTTTACGAATCTC 2124 0 1834 .......................................................................................... 1923 0 2860 ........... 2850 1 277 ........... 287 N A Y T L H W Q V V G D H G K V I T D G T R R V D A A P H A Query_1 2125 AATGCTTATACCTTACACTGGCAGGTGGTAGGTGATCATGGGAAAGTGATTACTGATGGTACTCGTCGGGTAGATGCCGCTCCGCACGCC 2214 0 1924 .......................................................................................... 2013 1 1145 ... 1143 T V E V T L G A V R L P S D V R E A Y L N L S W T Query_1 2215 ACCGTTGAA-GTTACGCTGG-GT-GCT-GT-ACG-TTTA--CCTTCTGA-TGTT-CGTGAAGCTTA-TCTGAACTTAAGTTGG--ACGTC 2291 0 2014 .........-..........-..-...-..-...-....--........-....-...........-................--..... 2090 1 1142 .....N...A..........A..N..NN..N...G....AC.N......A....T...N.......N...........N....NA..... 1053 S V D A A P F I N T D Y E V A Y D Q F V L S G N K N Y R A Query_1 2292 TGT-GGATGCTGCTCCGTTTATTAATACGGATTACGAGGTA-GCCT-ATGACCAATTTGTTCTTT-CGGGTAATAAGAACTACC-GGGCT 2376 0 2091 ...-.....................................-....-..................-..................-..... 2175 1 1052 N..N.....................................A....T..................T..................N..... 963 P E T K L S G K V E M N I D P A T G A L H S Y I Y N G K E Query_1 2377 CCGGAAACTAAACTTTCGGGTAAGGTGGAAATGAATATAGATCCGGCAACAGGGGCGCTTCACTCTTATATTTATAATGGG-AAAGAATT 2465 0 2176 .................................................................................-........ 2264 1 962 .................................................................................N........ 873 L L V S P V T L S L Y R P V T D N D N R E K V G G A K A W K Query_1 2466 ACTGGTTTCCCCCGTTACATTGAGCCTCTATCGTCCGGTGACGGATAATGATAACCGTGAAAAAGTAGGAGGAGCCAAAGCTTGGAAGAA 2555 0 2265 .......................................................................................... 2354 1 872 .......................................................................................... 783 K A G L D Q L T Q R A L T V K T S T R S G R A E V E L L N V Query_1 2556 AGCTGGGTTGGATCAGTTAACACAGCGTGCCCTTACCGTGAAGACTTCTACCCGCAGTGGACGTGCCGAAGTGGAATTATTAAATGTCCG 2645 0 2355 .......................................................................................... 2444 1 782 .......................................................................................... 693 R G E K V G T A S F I Y T L K K N G E L D I Q T H F V P D T Query_1 2646 TGGTGAAAAAGTGGGAACCGCTTCTTTCATCTATACACTAAAGAAAAATGGCGAATTGGATATACAGACACACTTTGTGCCGGATACTAC 2735 0 2445 .......................................................................................... 2534 1 692 .......................................................................................... 603 T V I T S L A R V G L T F E M P D A Y N Q V S Y L G R G E H Query_1 2736 CGTTATCACTTCACTGGCGCGTGTAGGGCTTACTTTTGAAATGCCCGATGCTTACAATCAGGTGTCTTATCTGGGGCGTGGAGAGCATGA 2825 0 2535 .......................................................................................... 2624 1 602 .......................................................................................... 513 E T Y M D R N Q S G K I G I Y H T D V E S M F H Y Y V R P Q Query_1 2826 AACTTATATGGATCGTAATCAATCCGGTAAAATAGGTATTTATCACACCGATGTGGAGAGTATGTTCCACTATTATGTTCGTCCGCAGGC 2915 0 2625 .......................................................................................... 2714 1 512 .......................................................................................... 423 A T G N R T D V R W M Q L A D E A G E G L A F R S D A S F Q Query_1 2916 AACGGGTAACCGTACGGATGTCCGTTGGATGCAACTTGCTGATGAGGCAGGTGAGGGGCTGGCATTCCGTTCCGATGCCTCGTTTCAGTT 3005 0 2715 .......................................................................................... 2804 1 422 .......................................................................................... 333 F S V I P F T D E N L D A A T H I N Q L R R D G V V T V H L Query_1 3006 TAGTGTGATTCCTTTTACGGATGAGAATCTGGATGCGGCAACGCATATCAATCAATTACGGCGCGATGGAGTGGTAACTGTTCATCTGGA 3095 0 2805 .......................................................................................... 2894 1 332 .......................................................................................... 243 D A A Q A G V G T A T C G P G V L P Q Y R V P V Q D Y I F R Query_1 3096 TGCCGCACAGGCAGGAGTCGGTACGGCTACCTGTGGTCCTGGAGTATTGCCGCAGTATCGTGTACCTGTGCAGGATTACATATTCCGGTT 3185 0 2895 .......................................................................................... 2984 0 537 ........... 547 1 242 .......................................................................................... 153 2 689 ........... 699 F T I R P L K * H W K W I T D P N S S S V D K L A A A L E H Query_1 3186 TACAATCCGTCCTTTGAAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcacca 3275 0 2985 ................... 3003 1 152 .....................................................................NN................... 63 H H H H H * D P A A N K A R K Query_1 3276 ccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGG 3319 1 62 ...........................N................ 19 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 18268710 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 5,443 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200020.108 (CMR200020.21-1021.P(1021).E480Q.pMCSG68)
QTVQPEWQSQYAIGKNKLAPHTYIWPYANADAAGEGNYEQSPYYMSLNGKWKFHWVKNPDHRPKDFYKPSFYTGGWADIDVPGNWERQGYGTAIYVNETYEFDDPLFNFKKNPPLVPYAENEVGSYRRTFTVPADWKDRRIVICCEGVISFYYIWVNGHMLGYNQGSKTPAEWDITDYVQAGENIVALEVYRWSAGSYLECQDMWRLSGIERDVYLYSTPRQFIADYKVTSSLDKKEYKEGFFGLDVTVEGSATGVSTLAYSLEDTAGKTILQNEIPIKNRGLSNSIIFDEQSLPGVKRWSAEHPNLYTLILTLKDDAGHITHLTGCKVGFRTSEIKDGRFCINGVPILIKGANRHEHSQLGRTVSKELMEEDIRLMKQHNLNTVRNSHYPTHPYWYQLCDRYGLYVIDEANIESHGMGYGPASLAKDSTWLPAHMDRTQRMYERAKNHPGIVIWSLGNEAGNGINFERTYDWMKSVETTRPIQYERAEQNYNTDIYCRMYRSVEELLAYARQTEPKVYRPFIMTEYLHAMGNSGGGLKEYMDVFETEPIVQGGCIWDWVDQSFREIDADGRWYWTYGGDYGPKNVPSFGNFCCNGLVNAVREPHPHLLEVKKAYQYIKTTLIDKSNLTLSIKNWYDFTNLNAYTLHWQVVGDHGKVITDGTRRVDAAPHATVEVTLGAVRLPSDVREAYLNLSWTSVDAAPFINTDYEVAYDQFVLSGNKNYRAPETKLSGKVEMNIDPATGALHSYIYNGKELLVSPVTLSLYRPVTDNDNREKVGGAKAWKKAGLDQLTQRALTVKTSTRSGRAEVELLNVRGEKVGTASFIYTLKKNGELDIQTHFVPDTTVITSLARVGLTFEMPDAYNQVSYLGRGEHETYMDRNQSGKIGIYHTDVESMFHYYVRPQATGNRTDVRWMQLADEAGEGLAFRSDASFQFSVIPFTDENLDAATHINQLRRDGVVTVHLDAAQAGVGTATCGPGVLPQYRVPVQDYIFRFTIRPLK
CAGACTGTACAGCCGGAATGGCAGAGCCAGTATGCTATCGGAAAAAACAAACTGGCACCACATACTTATATATGGCCTTATGCGAATGCTGATGCTGCGGGTGAAGGGAATTATGAGCAATCACCTTATTATATGAGTCTGAACGGTAAGTGGAAATTCCACTGGGTGAAGAATCCCGATCATCGTCCGAAAGACTTTTATAAACCTTCGTTCTATACGGGCGGATGGGCAGATATCGATGTACCCGGTAACTGGGAGCGTCAGGGATATGGTACGGCTATTTATGTGAACGAAACTTATGAATTTGATGATCCTTTGTTCAACTTCAAAAAGAATCCTCCGCTGGTACCTTATGCAGAGAACGAAGTAGGTTCTTATCGCCGTACGTTCACGGTTCCTGCTGACTGGAAAGACCGTCGCATCGTGATTTGCTGTGAAGGTGTGATCTCTTTCTATTATATCTGGGTGAACGGACATATGCTGGGTTATAACCAGGGTTCAAAGACTCCTGCCGAATGGGATATCACGGACTATGTACAGGCAGGAGAAAATATCGTGGCACTTGAAGTATATCGCTGGAGTGCAGGTTCCTATCTGGAGTGTCAGGATATGTGGCGGTTGAGTGGCATTGAACGGGATGTCTATCTTTACAGTACTCCACGCCAGTTTATAGCTGATTATAAAGTGACTTCTTCTTTGGATAAGAAAGAGTATAAGGAAGGATTTTTCGGCCTTGATGTAACAGTTGAAGGAAGTGCGACTGGTGTGTCTACTTTGGCATATAGTCTGGAAGATACTGCCGGAAAGACAATATTACAAAATGAAATACCGATCAAGAACCGTGGTTTGAGTAATTCTATTATCTTTGACGAACAGTCTCTTCCAGGTGTAAAACGTTGGAGTGCAGAACATCCGAATCTTTATACGTTAATATTGACGTTGAAAGATGATGCAGGACATATAACACACCTGACCGGTTGCAAAGTTGGTTTCCGTACTTCTGAAATCAAGGATGGCCGTTTTTGTATCAACGGGGTACCTATATTGATAAAAGGTGCTAACCGGCATGAACATTCACAGTTGGGACGTACTGTCAGCAAAGAACTGATGGAAGAAGACATTCGCTTGATGAAGCAACATAACCTGAATACTGTGCGCAACTCGCATTATCCGACACATCCGTACTGGTATCAACTCTGCGACCGTTACGGCCTTTATGTGATAGATGAAGCAAACATCGAATCGCATGGTATGGGGTATGGCCCGGCTTCATTGGCAAAGGATAGCACTTGGCTGCCTGCACACATGGACCGTACGCAACGTATGTACGAACGTGCCAAGAACCATCCGGGGATCGTGATCTGGTCGTTGGGCAACGAAGCGGGTAACGGAATCAATTTTGAACGGACATATGATTGGATGAAGTCCGTTGAAACCACCCGCCCTATACAATACGAGCGTGCCGAACAGAATTATAATACGGATATCTACTGCCGTATGTACCGCAGTGTGGAAGAATTGCTGGCCTATGCCCGCCAGACAGAACCGAAAGTGTATCGCCCTTTCATCATGACTGAATATCTGCATGCTATGGGCAATAGCGGAGGCGGATTGAAAGAATACATGGATGTTTTCGAAACAGAACCTATAGTGCAGGGCGGTTGTATCTGGGATTGGGTAGATCAGTCTTTCCGTGAAATAGATGCCGATGGTCGTTGGTATTGGACTTACGGTGGTGATTATGGTCCGAAGAATGTACCCAGTTTCGGGAACTTTTGCTGCAATGGTCTGGTGAATGCTGTCCGCGAACCTCATCCGCATTTGCTGGAAGTGAAGAAAGCATATCAATATATAAAGACTACATTGATTGATAAGAGCAACTTGACTTTAAGTATAAAGAATTGGTATGATTTTACGAATCTCAATGCTTATACCTTACACTGGCAGGTGGTAGGTGATCATGGGAAAGTGATTACTGATGGTACTCGTCGGGTAGATGCCGCTCCGCACGCCACCGTTGAAGTTACGCTGGGTGCTGTACGTTTACCTTCTGATGTTCGTGAAGCTTATCTGAACTTAAGTTGGACGTCTGTGGATGCTGCTCCGTTTATTAATACGGATTACGAGGTAGCCTATGACCAATTTGTTCTTTCGGGTAATAAGAACTACCGGGCTCCGGAAACTAAACTTTCGGGTAAGGTGGAAATGAATATAGATCCGGCAACAGGGGCGCTTCACTCTTATATTTATAATGGGAAAGAATTACTGGTTTCCCCCGTTACATTGAGCCTCTATCGTCCGGTGACGGATAATGATAACCGTGAAAAAGTAGGAGGAGCCAAAGCTTGGAAGAAAGCTGGGTTGGATCAGTTAACACAGCGTGCCCTTACCGTGAAGACTTCTACCCGCAGTGGACGTGCCGAAGTGGAATTATTAAATGTCCGTGGTGAAAAAGTGGGAACCGCTTCTTTCATCTATACACTAAAGAAAAATGGCGAATTGGATATACAGACACACTTTGTGCCGGATACTACCGTTATCACTTCACTGGCGCGTGTAGGGCTTACTTTTGAAATGCCCGATGCTTACAATCAGGTGTCTTATCTGGGGCGTGGAGAGCATGAAACTTATATGGATCGTAATCAATCCGGTAAAATAGGTATTTATCACACCGATGTGGAGAGTATGTTCCACTATTATGTTCGTCCGCAGGCAACGGGTAACCGTACGGATGTCCGTTGGATGCAACTTGCTGATGAGGCAGGTGAGGGGCTGGCATTCCGTTCCGATGCCTCGTTTCAGTTTAGTGTGATTCCTTTTACGGATGAGAATCTGGATGCGGCAACGCATATCAATCAATTACGGCGCGATGGAGTGGTAACTGTTCATCTGGATGCCGCACAGGCAGGAGTCGGTACGGCTACCTGTGGTCCTGGAGTATTGCCGCAGTATCGTGTACCTGTGCAGGATTACATATTCCGGTTTACAATCCGTCCTTTGAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,856 total letters Query= CMR200020.108 Length=3408 Score E Sequences producing significant alignments: (Bits) Value 0_wt 5407 0.0 1_02F_F5_CMR200020.108_T7term.seq 1813 0.0 2_05F_F5_CMR200020.108_T7S.seq 1180 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 58 TTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCAT-CATTCTTCTGGTGTAGATCTGTG 146 2 8 ..........................-..................................C..N.TN...................... 96 W S H P Q F E K G T E N L Y F Q S N A Q T V Q P E W Q S Q Y Query_1 147 GTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAGACTGTACAGCCGGAATGGCAGAGCCAGTATGC 236 0 1 ................................... 35 2 97 .......................................................................................... 186 A I G K N K L A P H T Y I W P Y A N A D A A G E G N Y E Q S Query_1 237 TATCGGAAAAAACAAACTGGCACCACATACTTATATATGGCCTTATGCGAATGCTGATGCTGCGGGTGAAGGGAATTATGAGCAATCACC 326 0 36 .......................................................................................... 125 2 187 .......................................................................................... 276 P Y Y M S L N G K W K F H W V K N P D H R P K D F Y K P S F Query_1 327 TTATTATATGAGTCTGAACGGTAAGTGGAAATTCCACTGGGTGAAGAATCCCGATCATCGTCCGAAAGACTTTTATAAACCTTCGTTCTA 416 0 126 .......................................................................................... 215 2 277 .......................................................................................... 366 Y T G G W A D I D V P G N W E R Q G Y G T A I Y V N E T Y E Query_1 417 TACGGGCGGATGGGCAGATATCGATGTACCCGGTAACTGGGAGCGTCAGGGATATGGTACGGCTATTTATGTGAACGAAACTTATGAATT 506 0 216 .......................................................................................... 305 2 367 .................G........................................................................ 456 F D D P L F N F K K N P P L V P Y A E N E V G S Y R R T F T Query_1 507 TGATGATCCTTTGTTCAACTTCAAAAAGAATCCTCCGCTGGTACCTTATGCAGAGAACGAAGTAGGTTCTTATCGCCGTACGTTCACGGT 596 0 306 .......................................................................................... 395 2 457 .......................................................................................... 546 V P A D W K D R R I V I C C E G V I S F Y Y I W V N G H M L Query_1 597 TCCTGCTGACTGGAAAGACCGTCGCATCGTGATTTGCTGTGAAGGTGTGATCTCTTTCTATTATATCTGGGTGAACGGACATATGCTGGG 686 0 396 .......................................................................................... 485 0 1401 .............. 1414 2 547 .......................................................................................... 636 G Y N Q G S K T P A E W D I T D Y V Q A G E N I V A L E V Y Query_1 687 TTATAACCAGGGTTCAAAGACTCCTGCCGAATGGGATATCACGGACTATGTACAGGCAGGAGAAAATATCGTGGCACTTGAAGTATATCG 776 0 486 .......................................................................................... 575 0 2901 ........... 2911 1 236 ........... 226 2 637 N.......................................N..... 682 R W S A G S Y L E C Q D M W R L S G I E R D V Y L Y S T P R Query_1 777 CTGGAGTGCAGGTTCCTATCTGGAGTGTCAGGATATGTGGCGGTTGAGTGGCATTGAACGGGATGTCTATCTTTACAGTACTCCACGCCA 866 0 576 .......................................................................................... 665 Q F I A D Y K V T S S L D K K E Y K E G F F G L D V T V E G Query_1 867 GTTTATAGCTGATTATAAAGTGACTTCTTCTTTGGATAAGAAAGAGTATAAGGAAGGATTTTTCGGCCTTGATGTAACAGTTGAAGGAAG 956 0 666 .......................................................................................... 755 S A T G V S T L A Y S L E D T A G K T I L Q N E I P I K N R Query_1 957 TGCGACTGGTGTGTCTACTTTGGCATATAGTCTGGAAGATACTGCCGGAAAGACAATATTACAAAATGAAATACCGATCAAGAACCGTGG 1046 0 756 .......................................................................................... 845 G L S N S I I F D E Q S L P G V K R W S A E H P N L Y T L I Query_1 1047 TTTGAGTAATTCTATTATCTTTGACGAACAGTCTCTTCCAGGTGTAAAACGTTGGAGTGCAGAACATCCGAATCTTTATACGTTAATATT 1136 0 846 .......................................................................................... 935 L T L K D D A G H I T H L T G C K V G F R T S E I K D G R F Query_1 1137 GACGTTGAAAGATGATGCAGGACATATAACACACCTGACCGGTTGCAAAGTTGGTTTCCGTACTTCTGAAATCAAGGATGGCCGTTTTTG 1226 0 936 .......................................................................................... 1025 C I N G V P I L I K G A N R H E H S Q L G R T V S K E L M E Query_1 1227 TATCAACGGGGTACCTATATTGATAAAAGGTGCTAACCGGCATGAACATTCACAGTTGGGACGTACTGTCAGCAAAGAACTGATGGAAGA 1316 0 1026 .......................................................................................... 1115 E D I R L M K Q H N L N T V R N S H Y P T H P Y W Y Q L C D Query_1 1317 AGACATTCGCTTGATGAAGCAACATAACCTGAATACTGTGCGCAACTCGCATTATCCGACACATCCGTACTGGTATCAACTCTGCGACCG 1406 0 1116 .......................................................................................... 1205 R Y G L Y V I D E A N I E S H G M G Y G P A S L A K D S T W Query_1 1407 TTACGGCCTTTATGTGATAGATGAAGCAAACATCGAATCGCATGGTATGGGGTATGGCCCGGCTTCATTGGCAAAGGATAGCACTTGGCT 1496 0 1206 .......................................................................................... 1295 L P A H M D R T Q R M Y E R A K N H P G I V I W S L G N Q A Query_1 1497 GCCTGCACACATGGACCGTACGCAACGTATGTACGAACGTGCCAAGAACCATCCGGGGATCGTGATCTGGTCGTTGGGCAACCAGGCGGG 1586 0 1296 ..................................................................................G.A..... 1385 G N G I N F E R T Y D W M K S V E T T R P I Q Y E R A E Q N Query_1 1587 TAACGGAATCAATTTTGAACGGACATATGATTGGATGAAGTCCGTTGAAACCACCCGCCCTATACAATACGAGCGTGCCGAACAGAATTA 1676 0 1386 .......................................................................................... 1475 0 467 .............. 480 2 618 .............. 631 Y N T D I Y C R M Y R S V E E L L A Y A R Q T E P K V Y R P Query_1 1677 TAATACGGATATCTACTGCCGTATGTACCGCAGTGTGGAAGAATTGCTGGCCTATGCCCGCCAGACAGAACCGAAAGTGTATCGCCCTTT 1766 0 1476 .......................................................................................... 1565 F I M T E Y L H A M G N S G G G L K E Y M D V F E T E P I V Query_1 1767 CATCATGACTGAATATCTGCATGCTATGGGCAATAGCGGAGGCGGATTGAAAGAATACATGGATGTTTTCGAAACAGAACCTATAGTGCA 1856 0 1566 .......................................................................................... 1655 Q G G C I W D W V D Q S F R E I D A D G R W Y W T Y G G D Y Query_1 1857 GGGCGGTTGTATCTGGGATTGGGTAGATCAGTCTTTCCGTGAAATAGATGCCGATGGTCGTTGGTATTGGACTTACGGTGGTGATTATGG 1946 0 1656 .......................................................................................... 1745 G P K N V P S F G N F C C N G L V N A V R E P H P H L L E V Query_1 1947 TCCGAAGAATGTACCCAGTTTCGGGAACTTTTGCTGCAATGGTCTGGTGAATGCTGTCCGCGAACCTCATCCGCATTTGCTGGAAGTGAA 2036 0 1746 .......................................................................................... 1835 1 979 .....NN....G..N...C.... 957 K K A Y Q Y I K T T L I D K S N L T L S I K N W Y D F T N L Query_1 2037 GAAAGCATATCAATATATAAAGACTACATTGATTGATAAGAGCAACTTGACTTTAAGTATAAAGAATTGGTATGATTTTACGAATCTCAA 2126 0 1836 .......................................................................................... 1925 0 2860 ........... 2850 1 277 ........... 287 N A Y T L H W Q V V G D H G K V I T D G T R R V D A A P H A Query_1 2127 TGCTTATACCTTACACTGGCAGGTGGTAGGTGATCATGGGAAAGTGATTACTGATGGTACTCGTCGGGTAGATGCCGCTCCGCACGCCAC 2216 0 1926 .......................................................................................... 2015 1 1148 ..... 1144 T V E V T L G A V R L P S D V R E A Y L N L S W T S Query_1 2217 C-GTTGAA---GTTACGCTGGG-TGCTGT-ACGTTTACCTT-CTG-ATGTTCGTGAAGCTTA--TCTGAACTTAAGTTGGACGT-CT-GT 2294 0 2016 .-......---...........-......-...........-...-................--....................-..-.. 2093 1 1143 .C....G.AAG......NN...G......T......N....T...N.......N.....C.NAT........N....G.N..NNN..N.. 1054 V D A A P F I N T D Y E V A Y D Q F V L S G N K N Y R A Query_1 2295 GGATGCTGCTCCGTTTA-TTAATACGGA-TTACG-AGGTAGCCT-ATGACCAATTTGTTCTTTC--GGGTAATAA-GAAC-TACCGGGCT 2376 0 2094 .................-..........-.....-.........-...................--.........-....-......... 2175 1 1053 .N...........G...A.........NN.....N.........T...................CG.........A...NN.....A.N. 964 P E T K L S G K V E M N I D P A T G A L H S Y I Y N G K E Query_1 2377 -CCGGAAACTAAACTTTCGGGTAAGGTGGAAATGAATATAGATCCGGCAACAGGGGCGCTTCACTCTTATATTTATAATGGGAAAGAATT 2465 0 2176 -......................................................................................... 2264 1 963 T......................................................................................... 874 L L V S P V T L S L Y R P V T D N D N R E K V G G A K A W K Query_1 2466 ACTGGTTTCCCCCGTTACATTGAGCCTCTATCGTCCGGTGACGGATAA-TGATAACCGTGAAAAAGTAGGAGGAGCCAAAGCTTGGAAGA 2554 0 2265 ................................................-......................................... 2353 1 873 ................................................A......................................... 784 K A G L D Q L T Q R A L T V K T S T R S G R A E V E L L N V Query_1 2555 AAGCTGGGTTGGATCAGTTAACACAGCGTGCCCTTACCGTGAAGACTTCTACCCGCAGTGGACGTGCCGAAGTGGAATTATTAAATGTCC 2644 0 2354 .......................................................................................... 2443 1 783 .......................................................................................... 694 R G E K V G T A S F I Y T L K K N G E L D I Q T H F V P D T Query_1 2645 GTGGTGAAAAAGTGGGAACCGCTTCTTTCATCTATACACTAAAGAAAAATGGCGAATTGGATATACAGACACACTTTGTGCCGGATACTA 2734 0 2444 .......................................................................................... 2533 1 693 .......................................................................................... 604 T V I T S L A R V G L T F E M P D A Y N Q V S Y L G R G E H Query_1 2735 CCGTTATCACTTCACTGGCGCGTGTAGGGCTTACTTTTGAAATGCCCGATGCTTACAATCAGGTGTCTTATCTGGGGCGTGGAGAGCATG 2824 0 2534 .......................................................................................... 2623 1 603 .......................................................................................... 514 E T Y M D R N Q S G K I G I Y H T D V E S M F H Y Y V R P Q Query_1 2825 AAACTTATATGGATCGTAATCAATCCGGTAAAATAGGTATTTATCACACCGATGTGGAGAGTATGTTCCACTATTATGTTCGTCCGCAGG 2914 0 2624 .......................................................................................... 2713 1 513 .......................................................................................... 424 A T G N R T D V R W M Q L A D E A G E G L A F R S D A S F Q Query_1 2915 CAACGGGTAACCGTACGGATGTCCGTTGGATGCAACTTGCTGATGAGGCAGGTGAGGGGCTGGCATTCCGTTCCGATGCCTCGTTTCAGT 3004 0 2714 .......................................................................................... 2803 1 423 .......................................................................................... 334 F S V I P F T D E N L D A A T H I N Q L R R D G V V T V H L Query_1 3005 TTAGTGTGATTCCTTTTACGGATGAGAATCTGGATGCGGCAACGCATATCAATCAATTACGGCGCGATGGAGTGGTAACTGTTCATCTGG 3094 0 2804 .......................................................................................... 2893 1 333 .......................................................................................... 244 D A A Q A G V G T A T C G P G V L P Q Y R V P V Q D Y I F R Query_1 3095 ATGCCGCACAGGCAGGAGTCGGTACGGCTACCTGTGGTCCTGGAGTATTGCCGCAGTATCGTGTACCTGTGCAGGATTACATATTCCGGT 3184 0 2894 .......................................................................................... 2983 0 537 ........... 547 1 243 .......................................................................................... 154 F T I R P L K * H W K W I T D P N S S S V D K L A A A L E H Query_1 3185 TTACAATCCGTCCTTTGAAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcacc 3274 0 2984 .................... 3003 1 153 .......................................................................................... 64 H H H H H * D P A A N K A R K E Query_1 3275 accaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAG 3322 1 63 ................................................ 16 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 16278780 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,856 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200020.109 (CMR200020.21-1021.P(1021).E546Q.pMCSG68)
QTVQPEWQSQYAIGKNKLAPHTYIWPYANADAAGEGNYEQSPYYMSLNGKWKFHWVKNPDHRPKDFYKPSFYTGGWADIDVPGNWERQGYGTAIYVNETYEFDDPLFNFKKNPPLVPYAENEVGSYRRTFTVPADWKDRRIVICCEGVISFYYIWVNGHMLGYNQGSKTPAEWDITDYVQAGENIVALEVYRWSAGSYLECQDMWRLSGIERDVYLYSTPRQFIADYKVTSSLDKKEYKEGFFGLDVTVEGSATGVSTLAYSLEDTAGKTILQNEIPIKNRGLSNSIIFDEQSLPGVKRWSAEHPNLYTLILTLKDDAGHITHLTGCKVGFRTSEIKDGRFCINGVPILIKGANRHEHSQLGRTVSKELMEEDIRLMKQHNLNTVRNSHYPTHPYWYQLCDRYGLYVIDEANIESHGMGYGPASLAKDSTWLPAHMDRTQRMYERAKNHPGIVIWSLGNEAGNGINFERTYDWMKSVETTRPIQYERAEQNYNTDIYCRMYRSVEELLAYARQTEPKVYRPFIMTEYLHAMGNSGGGLKEYMDVFETEPIVQGGCIWDWVDQSFREIDADGRWYWTYGGDYGPKNVPSFGNFCCNGLVNAVREPHPHLLEVKKAYQYIKTTLIDKSNLTLSIKNWYDFTNLNAYTLHWQVVGDHGKVITDGTRRVDAAPHATVEVTLGAVRLPSDVREAYLNLSWTSVDAAPFINTDYEVAYDQFVLSGNKNYRAPETKLSGKVEMNIDPATGALHSYIYNGKELLVSPVTLSLYRPVTDNDNREKVGGAKAWKKAGLDQLTQRALTVKTSTRSGRAEVELLNVRGEKVGTASFIYTLKKNGELDIQTHFVPDTTVITSLARVGLTFEMPDAYNQVSYLGRGEHETYMDRNQSGKIGIYHTDVESMFHYYVRPQATGNRTDVRWMQLADEAGEGLAFRSDASFQFSVIPFTDENLDAATHINQLRRDGVVTVHLDAAQAGVGTATCGPGVLPQYRVPVQDYIFRFTIRPLK
CAGACTGTACAGCCGGAATGGCAGAGCCAGTATGCTATCGGAAAAAACAAACTGGCACCACATACTTATATATGGCCTTATGCGAATGCTGATGCTGCGGGTGAAGGGAATTATGAGCAATCACCTTATTATATGAGTCTGAACGGTAAGTGGAAATTCCACTGGGTGAAGAATCCCGATCATCGTCCGAAAGACTTTTATAAACCTTCGTTCTATACGGGCGGATGGGCAGATATCGATGTACCCGGTAACTGGGAGCGTCAGGGATATGGTACGGCTATTTATGTGAACGAAACTTATGAATTTGATGATCCTTTGTTCAACTTCAAAAAGAATCCTCCGCTGGTACCTTATGCAGAGAACGAAGTAGGTTCTTATCGCCGTACGTTCACGGTTCCTGCTGACTGGAAAGACCGTCGCATCGTGATTTGCTGTGAAGGTGTGATCTCTTTCTATTATATCTGGGTGAACGGACATATGCTGGGTTATAACCAGGGTTCAAAGACTCCTGCCGAATGGGATATCACGGACTATGTACAGGCAGGAGAAAATATCGTGGCACTTGAAGTATATCGCTGGAGTGCAGGTTCCTATCTGGAGTGTCAGGATATGTGGCGGTTGAGTGGCATTGAACGGGATGTCTATCTTTACAGTACTCCACGCCAGTTTATAGCTGATTATAAAGTGACTTCTTCTTTGGATAAGAAAGAGTATAAGGAAGGATTTTTCGGCCTTGATGTAACAGTTGAAGGAAGTGCGACTGGTGTGTCTACTTTGGCATATAGTCTGGAAGATACTGCCGGAAAGACAATATTACAAAATGAAATACCGATCAAGAACCGTGGTTTGAGTAATTCTATTATCTTTGACGAACAGTCTCTTCCAGGTGTAAAACGTTGGAGTGCAGAACATCCGAATCTTTATACGTTAATATTGACGTTGAAAGATGATGCAGGACATATAACACACCTGACCGGTTGCAAAGTTGGTTTCCGTACTTCTGAAATCAAGGATGGCCGTTTTTGTATCAACGGGGTACCTATATTGATAAAAGGTGCTAACCGGCATGAACATTCACAGTTGGGACGTACTGTCAGCAAAGAACTGATGGAAGAAGACATTCGCTTGATGAAGCAACATAACCTGAATACTGTGCGCAACTCGCATTATCCGACACATCCGTACTGGTATCAACTCTGCGACCGTTACGGCCTTTATGTGATAGATGAAGCAAACATCGAATCGCATGGTATGGGGTATGGCCCGGCTTCATTGGCAAAGGATAGCACTTGGCTGCCTGCACACATGGACCGTACGCAACGTATGTACGAACGTGCCAAGAACCATCCGGGGATCGTGATCTGGTCGTTGGGCAACGAAGCGGGTAACGGAATCAATTTTGAACGGACATATGATTGGATGAAGTCCGTTGAAACCACCCGCCCTATACAATACGAGCGTGCCGAACAGAATTATAATACGGATATCTACTGCCGTATGTACCGCAGTGTGGAAGAATTGCTGGCCTATGCCCGCCAGACAGAACCGAAAGTGTATCGCCCTTTCATCATGACTGAATATCTGCATGCTATGGGCAATAGCGGAGGCGGATTGAAAGAATACATGGATGTTTTCGAAACAGAACCTATAGTGCAGGGCGGTTGTATCTGGGATTGGGTAGATCAGTCTTTCCGTGAAATAGATGCCGATGGTCGTTGGTATTGGACTTACGGTGGTGATTATGGTCCGAAGAATGTACCCAGTTTCGGGAACTTTTGCTGCAATGGTCTGGTGAATGCTGTCCGCGAACCTCATCCGCATTTGCTGGAAGTGAAGAAAGCATATCAATATATAAAGACTACATTGATTGATAAGAGCAACTTGACTTTAAGTATAAAGAATTGGTATGATTTTACGAATCTCAATGCTTATACCTTACACTGGCAGGTGGTAGGTGATCATGGGAAAGTGATTACTGATGGTACTCGTCGGGTAGATGCCGCTCCGCACGCCACCGTTGAAGTTACGCTGGGTGCTGTACGTTTACCTTCTGATGTTCGTGAAGCTTATCTGAACTTAAGTTGGACGTCTGTGGATGCTGCTCCGTTTATTAATACGGATTACGAGGTAGCCTATGACCAATTTGTTCTTTCGGGTAATAAGAACTACCGGGCTCCGGAAACTAAACTTTCGGGTAAGGTGGAAATGAATATAGATCCGGCAACAGGGGCGCTTCACTCTTATATTTATAATGGGAAAGAATTACTGGTTTCCCCCGTTACATTGAGCCTCTATCGTCCGGTGACGGATAATGATAACCGTGAAAAAGTAGGAGGAGCCAAAGCTTGGAAGAAAGCTGGGTTGGATCAGTTAACACAGCGTGCCCTTACCGTGAAGACTTCTACCCGCAGTGGACGTGCCGAAGTGGAATTATTAAATGTCCGTGGTGAAAAAGTGGGAACCGCTTCTTTCATCTATACACTAAAGAAAAATGGCGAATTGGATATACAGACACACTTTGTGCCGGATACTACCGTTATCACTTCACTGGCGCGTGTAGGGCTTACTTTTGAAATGCCCGATGCTTACAATCAGGTGTCTTATCTGGGGCGTGGAGAGCATGAAACTTATATGGATCGTAATCAATCCGGTAAAATAGGTATTTATCACACCGATGTGGAGAGTATGTTCCACTATTATGTTCGTCCGCAGGCAACGGGTAACCGTACGGATGTCCGTTGGATGCAACTTGCTGATGAGGCAGGTGAGGGGCTGGCATTCCGTTCCGATGCCTCGTTTCAGTTTAGTGTGATTCCTTTTACGGATGAGAATCTGGATGCGGCAACGCATATCAATCAATTACGGCGCGATGGAGTGGTAACTGTTCATCTGGATGCCGCACAGGCAGGAGTCGGTACGGCTACCTGTGGTCCTGGAGTATTGCCGCAGTATCGTGTACCTGTGCAGGATTACATATTCCGGTTTACAATCCGTCCTTTGAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,788 total letters Query= CMR200020.109 Length=3408 Score E Sequences producing significant alignments: (Bits) Value 0_wt 5407 0.0 1_02G_G5_CMR200020.109_T7term.seq 1638 0.0 2_05G_G5_CMR200020.109_T7S.seq 1366 0.0 N N F V * L * E G D I H M H H H H H H S S G V D L W S H P Query_1 69 AAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCA 158 2 14 ......N..............................................N.NN................................. 103 Q F E K G T E N L Y F Q S N A Q T V Q P E W Q S Q Y A I G K Query_1 159 GTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAGACTGTACAGCCGGAATGGCAGAGCCAGTATGCTATCGGAAAAAA 248 0 1 ............................................... 47 2 104 .......................................................................................... 193 N K L A P H T Y I W P Y A N A D A A G E G N Y E Q S P Y Y M Query_1 249 CAAACTGGCACCACATACTTATATATGGCCTTATGCGAATGCTGATGCTGCGGGTGAAGGGAATTATGAGCAATCACCTTATTATATGAG 338 0 48 .......................................................................................... 137 2 194 .......................................................................................... 283 S L N G K W K F H W V K N P D H R P K D F Y K P S F Y T G G Query_1 339 TCTGAACGGTAAGTGGAAATTCCACTGGGTGAAGAATCCCGATCATCGTCCGAAAGACTTTTATAAACCTTCGTTCTATACGGGCGGATG 428 0 138 .......................................................................................... 227 2 284 .......................................................................................... 373 W A D I D V P G N W E R Q G Y G T A I Y V N E T Y E F D D P Query_1 429 GGCAGATATCGATGTACCCGGTAACTGGGAGCGTCAGGGATATGGTACGGCTATTTATGTGAACGAAACTTATGAATTTGATGATCCTTT 518 0 228 .......................................................................................... 317 2 374 .....G.................................................................................... 463 L F N F K K N P P L V P Y A E N E V G S Y R R T F T V P A D Query_1 519 GTTCAACTTCAAAAAGAATCCTCCGCTGGTACCTTATGCAGAGAACGAAGTAGGTTCTTATCGCCGTACGTTCACGGTTCCTGCTGACTG 608 0 318 .......................................................................................... 407 2 464 .......................................N.........N........................................ 553 W K D R R I V I C C E G V I S F Y Y I W V N G H M L G Y N Q Query_1 609 GAAAGACCGTCGCATCGTGATTTGCTGTGAAGGTGTGATCTCTTTCTATTATATCTGGGTGAACGGACATATGCTGGGTTATAACCAGGG 698 0 408 .......................................................................................... 497 0 1401 .............. 1414 2 554 .......................................................................................... 643 G S K T P A E W D I T D Y V Q A G E N I V A L E V Y R W S A Query_1 699 TTCAAAGACT-CCTGCCGAATGGGATATCACGGACTATGTACAGGCAGGAGAAAATATCGTGGCACTTGAAGTATATCGCTGGAGTGCAG 787 0 498 ..........-............................................................................... 586 0 2901 ........... 2911 1 236 ........... 226 2 644 ..........N............................................................................... 733 G S Y L E C Q D M W R L S G I E R D V Y L Y S T P R Q F I A Query_1 788 GTTCCTATCTGGAGTGTCAGGATATGTGGCGGTTGAGTGGCATTGAACGGGATGTCTATCTTTACAGTACTCCACGCCAGTTTATAGCTG 877 0 587 .......................................................................................... 676 2 734 ................-NN...........................................NN...N... 803 D Y K V T S S L D K K E Y K E G F F G L D V T V E G S A T G Query_1 878 ATTATAAAGTGACTTCTTCTTTGGATAAGAAAGAGTATAAGGAAGGATTTTTCGGCCTTGATGTAACAGTTGAAGGAAGTGCGACTGGTG 967 0 677 .......................................................................................... 766 V S T L A Y S L E D T A G K T I L Q N E I P I K N R G L S N Query_1 968 TGTCTACTTTGGCATATAGTCTGGAAGATACTGCCGGAAAGACAATATTACAAAATGAAATACCGATCAAGAACCGTGGTTTGAGTAATT 1057 0 767 .......................................................................................... 856 S I I F D E Q S L P G V K R W S A E H P N L Y T L I L T L K Query_1 1058 CTATTATCTTTGACGAACAGTCTCTTCCAGGTGTAAAACGTTGGAGTGCAGAACATCCGAATCTTTATACGTTAATATTGACGTTGAAAG 1147 0 857 .......................................................................................... 946 D D A G H I T H L T G C K V G F R T S E I K D G R F C I N G Query_1 1148 ATGATGCAGGACATATAACACACCTGACCGGTTGCAAAGTTGGTTTCCGTACTTCTGAAATCAAGGATGGCCGTTTTTGTATCAACGGGG 1237 0 947 .......................................................................................... 1036 V P I L I K G A N R H E H S Q L G R T V S K E L M E E D I R Query_1 1238 TACCTATATTGATAAAAGGTGCTAACCGGCATGAACATTCACAGTTGGGACGTACTGTCAGCAAAGAACTGATGGAAGAAGACATTCGCT 1327 0 1037 .......................................................................................... 1126 L M K Q H N L N T V R N S H Y P T H P Y W Y Q L C D R Y G L Query_1 1328 TGATGAAGCAACATAACCTGAATACTGTGCGCAACTCGCATTATCCGACACATCCGTACTGGTATCAACTCTGCGACCGTTACGGCCTTT 1417 0 1127 .......................................................................................... 1216 Y V I D E A N I E S H G M G Y G P A S L A K D S T W L P A H Query_1 1418 ATGTGATAGATGAAGCAAACATCGAATCGCATGGTATGGGGTATGGCCCGGCTTCATTGGCAAAGGATAGCACTTGGCTGCCTGCACACA 1507 0 1217 .......................................................................................... 1306 M D R T Q R M Y E R A K N H P G I V I W S L G N E A G N G I Query_1 1508 TGGACCGTACGCAACGTATGTACGAACGTGCCAAGAACCATCCGGGGATCGTGATCTGGTCGTTGGGCAACGAAGCGGGTAACGGAATCA 1597 0 1307 .......................................................................................... 1396 N F E R T Y D W M K S V E T T R P I Q Y E R A E Q N Y N T D Query_1 1598 ATTTTGAACGGACATATGATTGGATGAAGTCCGTTGAAACCACCCGCCCTATACAATACGAGCGTGCCGAACAGAATTATAATACGGATA 1687 0 1397 .......................................................................................... 1486 0 467 .............. 480 2 613 .............. 626 I Y C R M Y R S V E E L L A Y A R Q T E P K V Y R P F I M T Query_1 1688 TCTACTGCCGTATGTACCGCAGTGTGGAAGAATTGCTGGCCTATGCCCGCCAGACAGAACCGAAAGTGTATCGCCCTTTCATCATGACTC 1777 0 1487 .........................................................................................G 1576 Q Y L H A M G N S G G G L K E Y M D V F E T E P I V Q G G C Query_1 1778 AGTATCTGCATGCTATGGGCAATAGCGGAGGCGGATTGAAAGAATACATGGATGTTTTCGAAACAGAACCTATAGTGCAGGGCGGTTGTA 1867 0 1577 .A........................................................................................ 1666 I W D W V D Q S F R E I D A D G R W Y W T Y G G D Y G P K N Query_1 1868 TCTGGGATTGGGTAGATCAGTCTTTCCGTGAAATAGATGCCGATGGTCGTTGGTATTGGACTTACGGTGGTGATTATGGTCCGAAGAATG 1957 0 1667 .......................................................................................... 1756 V P S F G N F C C N G L V N A V R E P H P H L L E V K K A Y Query_1 1958 TACCCAGTTTCGGGAACTTTTGCTGCAATGGTCTGGTGAATGCTGTCCGCGAACCTCATCCGCATTTGCTGGAAGTGAAGAAAGCATATC 2047 0 1757 .......................................................................................... 1846 Q Y I K T T L I D K S N L T L S I K N W Y D F T N L N A Y T Query_1 2048 AATATATAAAGACTACATTGATTGATAAGAGCAACTTGACTTTAAGTATAAAGAATTGGTATGATTTTACGAATCTCAATGCTTATACCT 2137 0 1847 .......................................................................................... 1936 0 2860 ........... 2850 1 277 ........... 287 L H W Q V V G D H G K V I T D G T R R V D A A P H A T V E V Query_1 2138 TACACTGGCAGGTGGTAGGTGATCATGGGAAAGTGATTACTGATGGTACTCGTCGGGTAGATGCCGCTCCGCACGCCACCGTTGAAGTTA 2227 0 1937 .......................................................................................... 2026 T L G A V R L P S D V R E A Y L N L S W T S V D A A P F I N Query_1 2228 CGCTGGGTGCTGTACGTTTACCTTCTGATGTTCGTGAAGCTTATCTGAACTTAAGTTGGACGTCTGTGGATGCTGCTCCGTTTATTAATA 2317 0 2027 .......................................................................................... 2116 T D Y E V A Y D Q F V L S G N K N Y R A P E T K L S G K V E Query_1 2318 CGGATTACGAGGTAGCCTATGACCAATTTGTTCTTTCGGGTAATAAGAACTACCGGGCTCCGGAAAC-TAAACTTTCGGGTAAGGTGGAA 2406 0 2117 ...................................................................-...................... 2205 1 977 .......................N...N............N.-....A.. 929 M N I D P A T G A L H S Y I Y N G K E L L V S P V T L S L Query_1 2407 ATGAATATAGATCCGGCAACAGGGGCGCTTCACTCTTATATTTATAATGGGAAAGAATTACTGG-TTTCCCCCGTTACATTGAGCCTCTA 2495 0 2206 ................................................................-......................... 2294 1 928 ...............-........-............................GN...-.....G..........-....-......... 844 Y R P V T D N D N R E K V G G A K A W K K A G L D Q L T Q R Query_1 2496 TCGTCCGGTGACGGATAATGATAACCGTGAAAAAGTAGGAGGAGCCAAAGCTTGGAAGAAAGCTGGGTTGGATCAGTTAACACAGCGTGC 2585 0 2295 .......................................................................................... 2384 1 843 .......................................................................................... 754 A L T V K T S T R S G R A E V E L L N V R G E K V G T A S F Query_1 2586 CCTTACCGTGAAGACTTCTACCCGCAGTGGACGTGCCGAAGTGGAATTATTAAATGTCCGTGGTGAAAAAGTGGGAA-CCGCTTCTTTCA 2674 0 2385 .............................................................................-............ 2473 1 753 .............................................................................A............ 664 I Y T L K K N G E L D I Q T H F V P D T T V I T S L A R V G Query_1 2675 TCTATACACTAAAGAAAAATGGCGAATTGGATATACAGACACACTTTGTGCCGGATACTACCGTTATCACTTCACTGGCGCGTGTAGGGC 2764 0 2474 .......................................................................................... 2563 1 663 .......................................................................................... 574 L T F E M P D A Y N Q V S Y L G R G E H E T Y M D R N Q S G Query_1 2765 TTACTTTTGAAATGCCCGATGCTTACAATCAGGTGTCTTATCTGGGGCGTGGAGAGCATGAAACTTATATGGATCGTAATCAATCCGGTA 2854 0 2564 .......................................................................................... 2653 1 573 .........................................N................................................ 484 K I G I Y H T D V E S M F H Y Y V R P Q A T G N R T D V R W Query_1 2855 AAATAGGTATTTATCACACCGATGTGGAGAGTATGTTCCACTATTATGTTCGTCCGCAGGCAACGGGTAACCGTACGGATGTCCGTTGGA 2944 0 2654 .......................................................................................... 2743 1 483 .......................................................................................... 394 M Q L A D E A G E G L A F R S D A S F Q F S V I P F T D E N Query_1 2945 TGCAACTTGCTGATGAGGCAGGTGAGGGGCTGGCATTCCGTTCCGATGCCTCGTTTCAGTTTAGTGTGATTCCTTTTACGGATGAGAATC 3034 0 2744 .......................................................................................... 2833 1 393 .......................................................................................... 304 L D A A T H I N Q L R R D G V V T V H L D A A Q A G V G T A Query_1 3035 TGGATGCGGCAACGCATATCAATCAATTACGGCGCGATGGAGTGGTAACTGTTCATCTGGATGCCGCACAGGCAGGAGTCGGTACGGCTA 3124 0 2834 .......................................................................................... 2923 0 537 ........... 547 1 303 .......................................................................................... 214 T C G P G V L P Q Y R V P V Q D Y I F R F T I R P L K * H W Query_1 3125 CCTGTGGTCCTGGAGTATTGCCGCAGTATCGTGTACCTGTGCAGGATTACATATTCCGGTTTACAATCCGTCCTTTGAAATAACATTGGA 3214 0 2924 ................................................................................ 3003 1 213 .......................................................................................... 124 K W I T D P N S S S V D K L A A A L E H H H H H H * D P A A Query_1 3215 AGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTA 3304 1 123 .......................................................................................... 34 N K A R K E Query_1 3305 ACAAAGCCC-GAAAGGAA 3321 1 33 .........G....N... 16 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 16048260 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,788 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200027.123 (CMR200027.20-824.P(824).E502Q.pMCSG68)
QRSEYLLEKNWKFMKGEAPEAMKPEFDDRKWETVTVPHDWAIFGPFDRSNDLQEVAVTQNFEKKASVKTGRTGGLPYVGIGWYRTRFDAPVNQQTTLVFDGAMSEARVYVNGQEACFWPFGYNSFHCDVTGLLNKDGKNNTLAVRLENKPQSSRWYPGAGLYRNVRVVSTDKVHVPVWGTQLTTPHVSDEYASVRLLTTIANDEERDIRIVTEIISPDGKVVATKDNTRKINHGQPFEQNFLVNAPCLWSPETPYLYKAVSKIYADGKQTDEYTTRFGIRSIEIIADKGFFLNGKHRKFQGVCNHHDLGPLGAAINVAALRRQLTMLKDMGCDAIRTAHNMPAPELVQLCDEMGFMMMLEPFDEWDIAKCENGYHRYFNEWAERDMINMLHQFRNNPCVVMWSIGNEVPTQCSPVGYKVASFLQDICHREDPTRPVTCGMDQVTCVLANGFAAMIDVPGFNYRAHRYLEAYELLPQNIVLGSETSSTVSSRGVYKFPVEKRGDAKYDDHQSSGYDLEHCAWSNVPDEDFALADDYDWTIGQFVWTGFDYLGEPSPYDTDAWPSHSSLFGIIDLASLPKDRYYLYRSLWNKNVNTLHILPHWTWPGREGENTPVFVYTNYPAAELFVNGKSYGKQHKLTAEESKAIQDKDTLALQRRYRLMWMDVPYEPGEVKVVAYDASGKPAEEKVVRTSGKPHHLEVIADRDQLTADGKDLAYITVRVVDKDGNLCPADNRLVNFTVKGAGRYRAAANGDATSLDLFHLPKMPAFSGQLTAIVQMTEQPGEIIFEAKAKGVKSGKLVLRSVRE
CAGCGCAGTGAGTATCTACTTGAAAAGAACTGGAAGTTCATGAAGGGGGAAGCTCCGGAAGCCATGAAGCCGGAATTTGACGACCGGAAGTGGGAAACCGTAACCGTGCCTCACGACTGGGCCATTTTTGGTCCCTTCGATCGCAGCAACGATTTGCAGGAAGTGGCGGTAACGCAGAACTTCGAGAAGAAAGCTTCCGTCAAGACCGGACGTACCGGTGGACTTCCTTATGTTGGCATCGGATGGTATCGTACTAGGTTCGATGCCCCCGTCAATCAACAGACGACACTTGTCTTTGATGGTGCCATGAGCGAAGCCCGTGTATATGTCAATGGACAAGAAGCATGCTTCTGGCCATTTGGTTATAATTCTTTCCATTGTGATGTCACCGGACTTTTGAATAAAGACGGTAAAAACAATACGCTTGCCGTGCGTTTGGAAAATAAACCACAATCTTCCCGTTGGTATCCTGGCGCAGGACTTTATCGCAATGTGCGTGTAGTGAGTACCGATAAAGTACATGTTCCTGTATGGGGTACTCAGCTGACTACTCCTCATGTTTCTGATGAGTATGCTTCAGTACGTCTGTTGACCACTATTGCCAATGATGAAGAAAGAGATATCCGTATCGTGACAGAGATAATCTCTCCCGATGGGAAAGTCGTTGCAACGAAGGATAATACCCGTAAGATTAATCATGGTCAGCCTTTTGAACAAAACTTCCTGGTGAATGCTCCTTGCTTGTGGTCGCCGGAGACACCTTATTTATATAAAGCTGTTTCTAAAATCTATGCCGATGGCAAGCAAACGGATGAATACACTACTCGTTTCGGCATCCGCAGCATAGAAATCATTGCCGACAAAGGATTTTTCCTGAACGGTAAGCATCGCAAGTTCCAGGGGGTGTGCAATCACCACGATCTTGGTCCGTTAGGCGCTGCCATCAATGTTGCTGCATTGCGCCGTCAACTTACGATGCTGAAAGATATGGGTTGTGATGCCATCCGCACCGCTCACAATATGCCGGCACCGGAGTTAGTGCAACTTTGTGATGAAATGGGTTTTATGATGATGCTGGAACCTTTCGACGAATGGGACATTGCCAAATGTGAGAATGGCTATCACCGTTATTTCAACGAGTGGGCAGAACGTGATATGATAAATATGTTGCATCAGTTCCGCAACAATCCTTGTGTCGTAATGTGGAGTATCGGTAATGAAGTTCCTACCCAATGTAGTCCCGTAGGCTATAAAGTCGCTTCTTTCTTGCAGGATATCTGTCATCGTGAAGATCCGACACGTCCTGTTACTTGCGGCATGGATCAGGTGACTTGTGTTCTTGCTAATGGTTTTGCCGCCATGATTGATGTGCCCGGTTTTAATTATCGCGCACACCGTTATCTGGAAGCTTATGAACTGTTGCCGCAGAATATAGTACTTGGTTCTGAAACATCCTCTACCGTTAGTTCTCGTGGCGTATATAAATTTCCTGTAGAGAAACGCGGGGATGCGAAGTACGATGATCACCAGTCTTCCGGATATGACTTGGAGCATTGTGCCTGGTCTAATGTTCCAGATGAAGATTTTGCTTTAGCGGATGATTATGACTGGACTATCGGTCAATTCGTTTGGACAGGATTCGATTATCTGGGTGAGCCTTCTCCTTATGATACGGATGCATGGCCAAGTCATAGCTCTTTGTTTGGTATCATTGACCTTGCCAGTTTGCCAAAAGACCGCTACTATCTGTACCGTAGTCTTTGGAATAAGAATGTGAATACACTCCATATACTTCCTCACTGGACATGGCCGGGTAGGGAAGGAGAGAATACTCCTGTCTTTGTTTACACAAACTATCCTGCTGCCGAACTTTTCGTTAATGGAAAAAGCTATGGTAAACAGCATAAACTGACAGCCGAAGAGAGTAAAGCTATTCAGGACAAAGATACACTTGCCCTCCAGCGTCGTTACCGCCTGATGTGGATGGACGTTCCTTATGAGCCGGGTGAAGTGAAAGTGGTGGCTTACGATGCTTCCGGCAAACCTGCTGAAGAAAAAGTAGTTCGTACTTCCGGCAAACCTCATCATCTGGAAGTCATTGCTGACCGTGACCAACTCACTGCCGATGGTAAAGATTTGGCATACATCACTGTTCGTGTGGTTGATAAAGACGGAAACCTTTGTCCTGCTGATAATCGTCTTGTAAACTTTACGGTGAAAGGCGCGGGGCGTTATCGTGCTGCCGCTAATGGAGATGCAACTTCACTTGATTTATTCCACTTGCCGAAGATGCCCGCTTTCAGTGGTCAGCTGACAGCCATTGTTCAAATGACCGAACAGCCCGGTGAAATTATTTTCGAGGCTAAGGCTAAAGGGGTGAAATCTGGTAAGCTTGTGCTGAGGTCTGTTAGAGAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,252 total letters Query= CMR200027.123 Length=2820 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4347 0.0 2_F3_CMR200027.123Seq171F09.seq 1710 0.0 1_03F_CMR200027.123Seq171F03.seq 1332 0.0 I V S G * Q F P S R N N F V * L * E G D I H M H H H H H H S Query_1 40 ATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCT 129 0 1020 ........... 1010 2 511 ........... 521 S G V D L W S H P Q F E K G T E N L Y F Q S N A Q R S E Y L Query_1 130 TCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAGCGCAGTGAGTATCTA 219 0 1 .................. 18 L E K N W K F M K G E A P E A M K P E F D D R K W E T V T V Query_1 220 CTTGAAAAGAACTGGAAGTTCATGAAGGGGGAAGCTCCGGAAGCCATGAAGCCGGAATTTGACGACCGGAAGTGGGAAACCGTAACCGTG 309 0 19 .......................................................................................... 108 P H D W A I F G P F D R S N D L Q E V A V T Q N F E K K A S Query_1 310 CCTCACGACTGGGCCATTTTTGGTCCCTTCGATCGCAGCAACGATTTGCAGGAAGTGGCGGTAACGCAGAACTTCGAGAAGAAAGCTTCC 399 0 109 .......................................................................................... 198 V K T G R T G G L P Y V G I G W Y R T R F D A P V N Q Q T T Query_1 400 GTCAAGACCGGACGTACCGGTGGACTTCCTTATGTTGGCATCGGATGGTATCGTACTAGGTTCGATGCCCCCGTCAATCAACAGACGACA 489 0 199 .......................................................................................... 288 L V F D G A M S E A R V Y V N G Q E A C F W P F G Y N S F Query_1 490 CTTGTCTTTGATGGTGCCATGAGCGAAGCCCGTGTATATGTCAATGGACAAGAAGCATGCTTC-TGGCCATTTGGTTATAATTCTTTCCA 578 0 289 ...............................................................-.......................... 377 0 359 .....C..-............T..T..... 331 H C D V T G L L N K D G K N N T L A V R L E N K P Q S S R W Query_1 579 TTGTGATGTCACCGGACTTTTGAATAAAGACGGTAAAAACAATACGCTTGCCGTGCGTTTGGAAAATAAACCACAATCTTCCCGTTGGTA 668 0 378 .......................................................................................... 467 Y P G A G L Y R N V R V V S T D K V H V P V W G T Q L T T Query_1 669 TCCTGGCGCAGGA-CTTTATCGCAATGTGCGTGT-AGTGAGTAC-CGATAAAGTACATGTTCCTGTA-TGGGGTACTCA-GCTGACTACT 753 0 468 .............-....................-.........-......................-...........-.......... 552 2 1063 ......AN..NN...........N...N........AT..............G...C...G.A.....N...A..N....... 981 P H V S D E Y A S V R L L T T I A N D E E R D I R I V T E Query_1 754 CCTCATGTTTCTGATGA-GTATGCTTCAGTACGTCTGTTGACCACTATTGCC-AATGATGAAGAAAGAGATATCCGTATCGTGACAGAGA 841 0 553 .................-..................................-..................................... 640 2 980 .TC........C..N..N...N...........................N..C......N.N............-............... 892 I I S P D G K V V A T K D N T R K I N H G Q P F E Q N F L Query_1 842 TAATCTCTCCCGATGGGAAA-GTCGTTGCAACGAAGGATAATACCCGTAAGATT-AATCATGGTCAGCCTTTTGAACAAAACTTCCTGGT 929 0 641 ....................-.................................-................................... 728 0 673 ............ 662 2 891 ....................A................N.......-........W.........................CT.C...... 803 2 858 ............ 869 V N A P C L W S P E T P Y L Y K A V S K I Y A D G K Q T D E Query_1 930 GAATGCTCCTTGCTTGTGGTCGCCGGAGACACCTTATTTATATAAAGCTGTTTCTAAAATCTATGCCGATGGCAA-GCAAACGGATGAAT 1018 0 729 ...........................................................................-.............. 817 2 802 ......................................-....................................A.............. 714 Y T T R F G I R S I E I I A D K G F F L N G K H R K F Q G V Query_1 1019 ACACTACTCGTTTCGGCATCCGCAGCATAGAAATCATTGCCGACAAAGGATTTTTCCTGAACGGTAAGCATCGCAAGTTCCAGGGGGTGT 1108 0 818 .......................................................................................... 907 2 713 ...N...................................................................................... 624 C N H H D L G P L G A A I N V A A L R R Q L T M L K D M G C Query_1 1109 GCAATCACCACGATCTTGGTCCGTTAGGCGCTGCCATCAATGTTGCTGCATTGCGCCGTCAACTTACGATGCTGAAAGATATGGGTTGTG 1198 0 908 .......................................................................................... 997 0 2024 ........... 2034 2 623 .......................................................................................... 534 D A I R T A H N M P A P E L V Q L C D E M G F M M M L E P F Query_1 1199 ATGCCATCCGCACCGCTCACAATATGCCGGCACCGGAGTTAGTGCAACTTTGTGATGAAATGGGTTTTATGATGATGCTGGAACCTTTCG 1288 0 998 .......................................................................................... 1087 2 533 .......................................................................................... 444 D E W D I A K C E N G Y H R Y F N E W A E R D M I N M L H Q Query_1 1289 ACGAATGGGACATTGCCAAATGTGAGAATGGCTATCACCGTTATTTCAACGAGTGGGCAGAACGTGATATGATAAATATGTTGCATCAGT 1378 0 1088 .......................................................................................... 1177 2 443 .......................................................................................... 354 1 2 ......-......................................... 48 F R N N P C V V M W S I G N E V P T Q C S P V G Y K V A S F Query_1 1379 TCCGCAACAATCCTTGTGTCGTAATGTGGAGTATCGGTAATGAAGTTCCTACCCAATGTAGTCCCGTAGGCTATAAAGTCGCTTCTTTCT 1468 0 1178 .......................................................................................... 1267 2 353 .......................................................................................... 264 1 49 .......................................................................................... 138 L Q D I C H R E D P T R P V T C G M D Q V T C V L A N G F A Query_1 1469 TGCAGGATATCTGTCATCGTGAAGATCCGACACGTCCTGTTACTTGCGGCATGGATCAGGTGACTTGTGTTCTTGCTAATGGTTTTGCCG 1558 0 1268 .......................................................................................... 1357 2 263 .......................................................................................... 174 1 139 .......................................................................................... 228 A M I D V P G F N Y R A H R Y L E A Y E L L P Q N I V L G S Query_1 1559 CCATGATTGATGTGCCCGGTTTTAATTATCGCGCACACCGTTATCTGGAAGCTTATGAACTGTTGCCGCAGAATATAGTACTTGGTTCTC 1648 0 1358 .........................................................................................G 1447 2 173 .......................................................................................... 84 1 229 .......................................................................................... 318 Q T S S T V S S R G V Y K F P V E K R G D A K Y D D H Q S S Query_1 1649 AGACATCCTCTACCGTTAGTTCTCGTGGCGTATATAAATTTCCTGTAGAGAAACGCGGGGATGCGAAGTACGATGATCACCAGTCTTCCG 1738 0 1448 .A........................................................................................ 1537 2 83 .........................................................................-N...... 4 1 319 .......................................................................................... 408 G Y D L E H C A W S N V P D E D F A L A D D Y D W T I G Q F Query_1 1739 GATATGACTTGGAGCATTGTGCCTGGTCTAATGTTCCAGATGAAGATTTTGCTTTAGCGGATGATTATGACTGGACTATCGGTCAATTCG 1828 0 1538 .......................................................................................... 1627 1 409 .......................................................................................... 498 V W T G F D Y L G E P S P Y D T D A W P S H S S L F G I I D Query_1 1829 TTTGGACAGGATTCGATTATCTGGGTGAGCCTTCTCCTTATGATACGGATGCATGGCCAAGTCATAGCTCTTTGTTTGGTATCATTGACC 1918 0 1628 .......................................................................................... 1717 1 499 .......................................................................................... 588 L A S L P K D R Y Y L Y R S L W N K N V N T L H I L P H W T Query_1 1919 TTGCCAG-TTTGCCAAAAGACCGCTACTATCTGTACCGTAGTCTTTGGAATAAGAATGTGAATACACTCCATATACTTCCTCACTGGACA 2007 0 1718 .......-.................................................................................. 1806 1 589 .......T.................................................................................. 678 W P G R E G E N T P V F V Y T N Y P A A E L F V N G K S Y G Query_1 2008 TGGCCGGGTAGGGAAGGAGAGAATACTCCTGTCTTTGTTTACACAAACTATCCTGCTGCCGAACTTTTCGTTAATGGAAAAAGCTATGGT 2097 0 1807 .......................................................................................... 1896 1 679 ..........N.........................................................TCN.T........ 759 K Q H K L T A E E S K A I Q D K D T L A L Q R R Y R L M W M Query_1 2098 AAACAGCATAAACTGACAGCCGAAGAGAGTAAAGCTATTCAGGACAAAGATACACTTGCCCTCCAGCGTCGTTACCGCCTGATGTGGATG 2187 0 1897 .......................................................................................... 1986 D V P Y E P G E V K V V A Y D A S G K P A E E K V V R T S G Query_1 2188 GACGTTCCTTATGAGCCGGGTGAAGTGAAAGTGGTGGCTTACGATGCTTCCGGCAAACCTGCTGAAGAAAAAGTAGTTCGTACTTCCGGC 2277 0 1987 .......................................................................................... 2076 0 970 ........... 980 2 561 ........... 551 K P H H L E V I A D R D Q L T A D G K D L A Y I T V R V V D Query_1 2278 AAACCTCATCATCTGGAAGTCATTGCTGACCGTGACCAACTCACTGCCGATGGTAAAGATTTGGCATACATCACTGTTCGTGTGGTTGAT 2367 0 2077 .......................................................................................... 2166 K D G N L C P A D N R L V N F T V K G A G R Y R A A A N G D Query_1 2368 AAAGACGGAAACCTTTGTCCTGCTGATAATCGTCTTGTAAACTTTACGGTGAAAGGCGCGGGGCGTTATCGTGCTGCCGCTAATGGAGAT 2457 0 2167 .......................................................................................... 2256 A T S L D L F H L P K M P A F S G Q L T A I V Q M T E Q P G Query_1 2458 GCAACTTCACTTGATTTATTCCACTTGCCGAAGATGCCCGCTTTCAGTGGTCAGCTGACAGCCATTGTTCAAATGACCGAACAGCCCGGT 2547 0 2257 .......................................................................................... 2346 E I I F E A K A K G V K S G K L V L R S V R E Query_1 2548 GAAATTATTTTCGAGGCTAAGGCTAAAGGGGTGAAATCTGGTAAGCTTGTGCTGAGGTCTGTTAGAGAG 2616 0 2347 ..................................................................... 2415 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11775403 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,252 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200027.124 (CMR200027.20-824.P(824).Y481A.pMCSG68)
QRSEYLLEKNWKFMKGEAPEAMKPEFDDRKWETVTVPHDWAIFGPFDRSNDLQEVAVTQNFEKKASVKTGRTGGLPYVGIGWYRTRFDAPVNQQTTLVFDGAMSEARVYVNGQEACFWPFGYNSFHCDVTGLLNKDGKNNTLAVRLENKPQSSRWYPGAGLYRNVRVVSTDKVHVPVWGTQLTTPHVSDEYASVRLLTTIANDEERDIRIVTEIISPDGKVVATKDNTRKINHGQPFEQNFLVNAPCLWSPETPYLYKAVSKIYADGKQTDEYTTRFGIRSIEIIADKGFFLNGKHRKFQGVCNHHDLGPLGAAINVAALRRQLTMLKDMGCDAIRTAHNMPAPELVQLCDEMGFMMMLEPFDEWDIAKCENGYHRYFNEWAERDMINMLHQFRNNPCVVMWSIGNEVPTQCSPVGYKVASFLQDICHREDPTRPVTCGMDQVTCVLANGFAAMIDVPGFNYRAHRYLEAYELLPQNIVLGSETSSTVSSRGVYKFPVEKRGDAKYDDHQSSGYDLEHCAWSNVPDEDFALADDYDWTIGQFVWTGFDYLGEPSPYDTDAWPSHSSLFGIIDLASLPKDRYYLYRSLWNKNVNTLHILPHWTWPGREGENTPVFVYTNYPAAELFVNGKSYGKQHKLTAEESKAIQDKDTLALQRRYRLMWMDVPYEPGEVKVVAYDASGKPAEEKVVRTSGKPHHLEVIADRDQLTADGKDLAYITVRVVDKDGNLCPADNRLVNFTVKGAGRYRAAANGDATSLDLFHLPKMPAFSGQLTAIVQMTEQPGEIIFEAKAKGVKSGKLVLRSVRE
CAGCGCAGTGAGTATCTACTTGAAAAGAACTGGAAGTTCATGAAGGGGGAAGCTCCGGAAGCCATGAAGCCGGAATTTGACGACCGGAAGTGGGAAACCGTAACCGTGCCTCACGACTGGGCCATTTTTGGTCCCTTCGATCGCAGCAACGATTTGCAGGAAGTGGCGGTAACGCAGAACTTCGAGAAGAAAGCTTCCGTCAAGACCGGACGTACCGGTGGACTTCCTTATGTTGGCATCGGATGGTATCGTACTAGGTTCGATGCCCCCGTCAATCAACAGACGACACTTGTCTTTGATGGTGCCATGAGCGAAGCCCGTGTATATGTCAATGGACAAGAAGCATGCTTCTGGCCATTTGGTTATAATTCTTTCCATTGTGATGTCACCGGACTTTTGAATAAAGACGGTAAAAACAATACGCTTGCCGTGCGTTTGGAAAATAAACCACAATCTTCCCGTTGGTATCCTGGCGCAGGACTTTATCGCAATGTGCGTGTAGTGAGTACCGATAAAGTACATGTTCCTGTATGGGGTACTCAGCTGACTACTCCTCATGTTTCTGATGAGTATGCTTCAGTACGTCTGTTGACCACTATTGCCAATGATGAAGAAAGAGATATCCGTATCGTGACAGAGATAATCTCTCCCGATGGGAAAGTCGTTGCAACGAAGGATAATACCCGTAAGATTAATCATGGTCAGCCTTTTGAACAAAACTTCCTGGTGAATGCTCCTTGCTTGTGGTCGCCGGAGACACCTTATTTATATAAAGCTGTTTCTAAAATCTATGCCGATGGCAAGCAAACGGATGAATACACTACTCGTTTCGGCATCCGCAGCATAGAAATCATTGCCGACAAAGGATTTTTCCTGAACGGTAAGCATCGCAAGTTCCAGGGGGTGTGCAATCACCACGATCTTGGTCCGTTAGGCGCTGCCATCAATGTTGCTGCATTGCGCCGTCAACTTACGATGCTGAAAGATATGGGTTGTGATGCCATCCGCACCGCTCACAATATGCCGGCACCGGAGTTAGTGCAACTTTGTGATGAAATGGGTTTTATGATGATGCTGGAACCTTTCGACGAATGGGACATTGCCAAATGTGAGAATGGCTATCACCGTTATTTCAACGAGTGGGCAGAACGTGATATGATAAATATGTTGCATCAGTTCCGCAACAATCCTTGTGTCGTAATGTGGAGTATCGGTAATGAAGTTCCTACCCAATGTAGTCCCGTAGGCTATAAAGTCGCTTCTTTCTTGCAGGATATCTGTCATCGTGAAGATCCGACACGTCCTGTTACTTGCGGCATGGATCAGGTGACTTGTGTTCTTGCTAATGGTTTTGCCGCCATGATTGATGTGCCCGGTTTTAATTATCGCGCACACCGTTATCTGGAAGCTTATGAACTGTTGCCGCAGAATATAGTACTTGGTTCTGAAACATCCTCTACCGTTAGTTCTCGTGGCGTATATAAATTTCCTGTAGAGAAACGCGGGGATGCGAAGTACGATGATCACCAGTCTTCCGGATATGACTTGGAGCATTGTGCCTGGTCTAATGTTCCAGATGAAGATTTTGCTTTAGCGGATGATTATGACTGGACTATCGGTCAATTCGTTTGGACAGGATTCGATTATCTGGGTGAGCCTTCTCCTTATGATACGGATGCATGGCCAAGTCATAGCTCTTTGTTTGGTATCATTGACCTTGCCAGTTTGCCAAAAGACCGCTACTATCTGTACCGTAGTCTTTGGAATAAGAATGTGAATACACTCCATATACTTCCTCACTGGACATGGCCGGGTAGGGAAGGAGAGAATACTCCTGTCTTTGTTTACACAAACTATCCTGCTGCCGAACTTTTCGTTAATGGAAAAAGCTATGGTAAACAGCATAAACTGACAGCCGAAGAGAGTAAAGCTATTCAGGACAAAGATACACTTGCCCTCCAGCGTCGTTACCGCCTGATGTGGATGGACGTTCCTTATGAGCCGGGTGAAGTGAAAGTGGTGGCTTACGATGCTTCCGGCAAACCTGCTGAAGAAAAAGTAGTTCGTACTTCCGGCAAACCTCATCATCTGGAAGTCATTGCTGACCGTGACCAACTCACTGCCGATGGTAAAGATTTGGCATACATCACTGTTCGTGTGGTTGATAAAGACGGAAACCTTTGTCCTGCTGATAATCGTCTTGTAAACTTTACGGTGAAAGGCGCGGGGCGTTATCGTGCTGCCGCTAATGGAGATGCAACTTCACTTGATTTATTCCACTTGCCGAAGATGCCCGCTTTCAGTGGTCAGCTGACAGCCATTGTTCAAATGACCGAACAGCCCGGTGAAATTATTTTCGAGGCTAAGGCTAAAGGGGTGAAATCTGGTAAGCTTGTGCTGAGGTCTGTTAGAGAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,343 total letters Query= CMR200027.124 Length=2820 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4347 0.0 2_G3_CMR200027.124Seq171G09.seq 1779 0.0 1_03G_CMR200027.124Seq171G03.seq 1272 0.0 I V S G * Q F P S R N N F V * L * E G D I H M H H H H H H S Query_1 40 ATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCT 129 0 1020 ........... 1010 2 515 ........... 525 S G V D L W S H P Q F E K G T E N L Y F Q S N A Q R S E Y L Query_1 130 TCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAGCGCAGTGAGTATCTA 219 0 1 .................. 18 L E K N W K F M K G E A P E A M K P E F D D R K W E T V T V Query_1 220 CTTGAAAAGAACTGGAAGTTCATGAAGGGGGAAGCTCCGGAAGCCATGAAGCCGGAATTTGACGACCGGAAGTGGGAAACCGTAACCGTG 309 0 19 .......................................................................................... 108 P H D W A I F G P F D R S N D L Q E V A V T Q N F E K K A S Query_1 310 CCTCACGACTGGGCCATTTTTGGTCCCTTCGATCGCAGCAACGATTTGCAGGAAGTGGCGGTAACGCAGAACTTCGAGAAGAAAGCTTCC 399 0 109 .......................................................................................... 198 V K T G R T G G L P Y V G I G W Y R T R F D A P V N Q Q T T Query_1 400 GTCAAGACCGGACGTACCGGTGGACTTCCTTATGTTGGCATCGGATGGTATCGTACTAGGTTCGATGCCCCCGTCAATCAACAGACGACA 489 0 199 .......................................................................................... 288 L V F D G A M S E A R V Y V N G Q E A C F W P F G Y N S F Query_1 490 CTTGTCTTTGATGGTGCCATGAGCGAAGCCCGTGTATATGTCAATGGACAAGAAGCATGCTTC-TGGCCATTTGGTTATAATTCTTTCCA 578 0 289 ...............................................................-.......................... 377 0 359 .....C..-............T..T..... 331 H C D V T G L L N K D G K N N T L A V R L E N K P Q S S R Query_1 579 TTGTGATGTCACCGGACTTTTGAATAAAG--ACGGTAAAAACAATACGCTTG-CCGTGC-GTTTGGAAAATAAACCACAAT-CTTCCCGT 663 0 378 .............................--.....................-......-.....................-........ 462 2 1157 .......GA.........C..-....N..NN......A..................C..T...G.... 1091 W Y P G A G L Y R N V R V V S T D K V H V P V W G T Q Query_1 664 TGGTATCCTGGCG-CAGGACTTT--ATCGC-AATG-TGCGTGTAGTGAG-TACCGAT--AAAGT-ACATGTTCCTGTATGGGGTACTCAG 744 0 463 .............-.........--.....-....-.............-.......--.....-......................... 543 2 1090 ..NNT...N..A.N.....AN..TA.....C....G......-..N...G..T...NTA.....G......N.........N........ 1002 L T T P H V S D E Y A S V R L L T T I A N D E E R D I R Query_1 745 CTGACTACTCCTCATGTTTCTGATG-AGTA-TGCTTCAGTACGTCTGTTGACCACTATTGCC-AATGATGAAGAAA-GAGATATCCGTAT 830 0 544 .........................-....-...............................-.............-............. 629 2 1001 N.........-...A..........N....A...............................C.............A............. 913 I V T E I I S P D G K V V A T K D N T R K I N H G Q P F E Query_1 831 CGTGACAGAGAT-AATCT-CTCCCGATGGGAAAGTCGTTGCAACGAA-GGATAATACCCGTAA-GATT-AATCATGGTCAGCCTTTTGAA 915 0 630 ............-.....-............................-...............-....-..................... 714 0 673 ............ 662 2 912 ............N.....T............................N...............A....N.....N............... 823 2 867 ............ 878 Q N F L V N A P C L W S P E T P Y L Y K A V S K I Y A D G Query_1 916 CAAAACTTCCTGGTGAATGCTCCTTGCTT-GTGGTCGCCGGAGACACCTTATTT-ATATAAAGCTGTTTCTAAAATCTATGCCGATGGCA 1003 0 715 .............................-........................-................................... 802 2 822 .............................T.................N......T................................... 733 K Q T D E Y T T R F G I R S I E I I A D K G F F L N G K H R Query_1 1004 AGCAAACGGATGAATACACTACTCGTTTCGGCATCCGCAGCATAGAAATCATTGCCGACAAAGGATTTTTCCTGAACGGTAAGCATCGCA 1093 0 803 .......................................................................................... 892 2 732 .......................................................................................... 643 K F Q G V C N H H D L G P L G A A I N V A A L R R Q L T M L Query_1 1094 AGTTCCAGGGGGTGTGCAATCACCACGATCTTGGTCCGTTAGGCGCTGCCATCAATGTTGCTGCATTGCGCCGTCAACTTACGATGCTGA 1183 0 893 .......................................................................................... 982 0 2024 ........... 2034 2 642 .......................................................................................... 553 K D M G C D A I R T A H N M P A P E L V Q L C D E M G F M M Query_1 1184 AAGATATGGGTTGTGATGCCATCCGCACCGCTCACAATATGCCGGCACCGGAGTTAGTGCAACTTTGTGATGAAATGGGTTTTATGATGA 1273 0 983 .......................................................................................... 1072 2 552 .......................................................................................... 463 M L E P F D E W D I A K C E N G Y H R Y F N E W A E R D M I Query_1 1274 TGCTGGAACCTTTCGACGAATGGGACATTGCCAAATGTGAGAATGGCTATCACCGTTATTTCAACGAGTGGGCAGAACGTGATATGATAA 1363 0 1073 .......................................................................................... 1162 2 462 .......................................................................................... 373 1 7 ........................... 33 N M L H Q F R N N P C V V M W S I G N E V P T Q C S P V G Y Query_1 1364 ATATGTTGCATCAGTTCCGCAACAATCCTTGTGTCGTAATGTGGAGTATCGGTAATGAAGTTCCTACCCAATGTAGTCCCGTAGGCTATA 1453 0 1163 .......................................................................................... 1252 2 372 .......................................................................................... 283 1 34 .......................................................................................... 123 K V A S F L Q D I C H R E D P T R P V T C G M D Q V T C V L Query_1 1454 AAGTCGCTTCTTTCTTGCAGGATATCTGTCATCGTGAAGATCCGACACGTCCTGTTACTTGCGGCATGGATCAGGTGACTTGTGTTCTTG 1543 0 1253 .......................................................................................... 1342 2 282 .......................................................................................... 193 1 124 .......................................................................................... 213 A N G F A A M I D V P G F N A R A H R Y L E A Y E L L P Q N Query_1 1544 CTAATGGTTTTGCCGCCATGATTGATGTGCCCGGTTTTAATGCTCGCGCACACCGTTATCTGGAAGCTTATGAACTGTTGCCGCAGAATA 1633 0 1343 .........................................TA............................................... 1432 2 192 .......................................................................................... 103 1 214 .......................................................................................... 303 I V L G S E T S S T V S S R G V Y K F P V E K R G D A K Y D Query_1 1634 TAGTACTTGGTTCTGAAACATCCTCTACCGTTAGTTCTCGTGGCGTATATAAATTTCCTGTAGAGAAACGCGGGGATGCG-AAGTACGAT 1722 0 1433 ................................................................................-......... 1521 2 102 ................................................................................A......... 13 1 304 ................................................................................-......... 392 D H Q S S G Y D L E H C A W S N V P D E D F A L A D D Y D W Query_1 1723 GATCACCAGTCTTCCGGATATGACTTGGAGCATTGTGCCTGGTCTAATGTTCCAGATGAAGATTTTGCTTTAGCGGATGATTATGACTGG 1812 0 1522 .......................................................................................... 1611 2 12 ....... 6 1 393 .......................................................................................... 482 T I G Q F V W T G F D Y L G E P S P Y D T D A W P S H S S L Query_1 1813 ACTATCGGTCAATTCGTTTGGACAGGATTCGATTATCTGGGTGAGCCTTCTCCTTATGATACGGATGCATGGCCAAGTCATAGCTCTTTG 1902 0 1612 .......................................................................................... 1701 1 483 .......................................................................................... 572 F G I I D L A S L P K D R Y Y L Y R S L W N K N V N T L H I Query_1 1903 TTTGGTATCATTGACCTTGCCAGTTTGCCAAAAGACCGCTACTATCTGTACCGTAGTCTTTGGAATAAGAATGTGAATACACTCCATATA 1992 0 1702 .......................................................................................... 1791 1 573 .......................................................................................... 662 L P H W T W P G R E G E N T P V F V Y T N Y P A A E L F V N Query_1 1993 CTTCCTCACTGGACATGGCCGGGTAGGGAAGGAGAGAATACTCCTGTCTTTGTTTACACAAACTATCCTGCTGCCGAACTTTTCGTTAAT 2082 0 1792 .......................................................................................... 1881 1 663 ....................-................................. 715 G K S Y G K Q H K L T A E E S K A I Q D K D T L A L Q R R Y Query_1 2083 GGAAAAAGCTATGGTAAACAGCATAAACTGACAGCCGAAGAGAGTAAAGCTATTCAGGACAAAGATACACTTGCCCTCCAGCGTCGTTAC 2172 0 1882 .......................................................................................... 1971 R L M W M D V P Y E P G E V K V V A Y D A S G K P A E E K V Query_1 2173 CGCCTGATGTGGATGGACGTTCCTTATGAGCCGGGTGAAGTGAAAGTGGTGGCTTACGATGCTTCCGGCAAACCTGCTGAAGAAAAAGTA 2262 0 1972 .......................................................................................... 2061 0 970 ........... 980 2 565 ........... 555 V R T S G K P H H L E V I A D R D Q L T A D G K D L A Y I T Query_1 2263 GTTCGTACTTCCGGCAAACCTCATCATCTGGAAGTCATTGCTGACCGTGACCAACTCACTGCCGATGGTAAAGATTTGGCATACATCACT 2352 0 2062 .......................................................................................... 2151 V R V V D K D G N L C P A D N R L V N F T V K G A G R Y R A Query_1 2353 GTTCGTGTGGTTGATAAAGACGGAAACCTTTGTCCTGCTGATAATCGTCTTGTAAACTTTACGGTGAAAGGCGCGGGGCGTTATCGTGCT 2442 0 2152 .......................................................................................... 2241 A A N G D A T S L D L F H L P K M P A F S G Q L T A I V Q M Query_1 2443 GCCGCTAATGGAGATGCAACTTCACTTGATTTATTCCACTTGCCGAAGATGCCCGCTTTCAGTGGTCAGCTGACAGCCATTGTTCAAATG 2532 0 2242 .......................................................................................... 2331 T E Q P G E I I F E A K A K G V K S G K L V L R S V R E Query_1 2533 ACCGAACAGCCCGGTGAAATTATTTTCGAGGCTAAGGCTAAAGGGGTGAAATCTGGTAAGCTTGTGCTGAGGTCTGTTAGAGAG 2616 0 2332 .................................................................................... 2415 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12030476 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,343 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200027.125 (CMR200027.20-824.P(824).Y481F.pMCSG68)
QRSEYLLEKNWKFMKGEAPEAMKPEFDDRKWETVTVPHDWAIFGPFDRSNDLQEVAVTQNFEKKASVKTGRTGGLPYVGIGWYRTRFDAPVNQQTTLVFDGAMSEARVYVNGQEACFWPFGYNSFHCDVTGLLNKDGKNNTLAVRLENKPQSSRWYPGAGLYRNVRVVSTDKVHVPVWGTQLTTPHVSDEYASVRLLTTIANDEERDIRIVTEIISPDGKVVATKDNTRKINHGQPFEQNFLVNAPCLWSPETPYLYKAVSKIYADGKQTDEYTTRFGIRSIEIIADKGFFLNGKHRKFQGVCNHHDLGPLGAAINVAALRRQLTMLKDMGCDAIRTAHNMPAPELVQLCDEMGFMMMLEPFDEWDIAKCENGYHRYFNEWAERDMINMLHQFRNNPCVVMWSIGNEVPTQCSPVGYKVASFLQDICHREDPTRPVTCGMDQVTCVLANGFAAMIDVPGFNYRAHRYLEAYELLPQNIVLGSETSSTVSSRGVYKFPVEKRGDAKYDDHQSSGYDLEHCAWSNVPDEDFALADDYDWTIGQFVWTGFDYLGEPSPYDTDAWPSHSSLFGIIDLASLPKDRYYLYRSLWNKNVNTLHILPHWTWPGREGENTPVFVYTNYPAAELFVNGKSYGKQHKLTAEESKAIQDKDTLALQRRYRLMWMDVPYEPGEVKVVAYDASGKPAEEKVVRTSGKPHHLEVIADRDQLTADGKDLAYITVRVVDKDGNLCPADNRLVNFTVKGAGRYRAAANGDATSLDLFHLPKMPAFSGQLTAIVQMTEQPGEIIFEAKAKGVKSGKLVLRSVRE
CAGCGCAGTGAGTATCTACTTGAAAAGAACTGGAAGTTCATGAAGGGGGAAGCTCCGGAAGCCATGAAGCCGGAATTTGACGACCGGAAGTGGGAAACCGTAACCGTGCCTCACGACTGGGCCATTTTTGGTCCCTTCGATCGCAGCAACGATTTGCAGGAAGTGGCGGTAACGCAGAACTTCGAGAAGAAAGCTTCCGTCAAGACCGGACGTACCGGTGGACTTCCTTATGTTGGCATCGGATGGTATCGTACTAGGTTCGATGCCCCCGTCAATCAACAGACGACACTTGTCTTTGATGGTGCCATGAGCGAAGCCCGTGTATATGTCAATGGACAAGAAGCATGCTTCTGGCCATTTGGTTATAATTCTTTCCATTGTGATGTCACCGGACTTTTGAATAAAGACGGTAAAAACAATACGCTTGCCGTGCGTTTGGAAAATAAACCACAATCTTCCCGTTGGTATCCTGGCGCAGGACTTTATCGCAATGTGCGTGTAGTGAGTACCGATAAAGTACATGTTCCTGTATGGGGTACTCAGCTGACTACTCCTCATGTTTCTGATGAGTATGCTTCAGTACGTCTGTTGACCACTATTGCCAATGATGAAGAAAGAGATATCCGTATCGTGACAGAGATAATCTCTCCCGATGGGAAAGTCGTTGCAACGAAGGATAATACCCGTAAGATTAATCATGGTCAGCCTTTTGAACAAAACTTCCTGGTGAATGCTCCTTGCTTGTGGTCGCCGGAGACACCTTATTTATATAAAGCTGTTTCTAAAATCTATGCCGATGGCAAGCAAACGGATGAATACACTACTCGTTTCGGCATCCGCAGCATAGAAATCATTGCCGACAAAGGATTTTTCCTGAACGGTAAGCATCGCAAGTTCCAGGGGGTGTGCAATCACCACGATCTTGGTCCGTTAGGCGCTGCCATCAATGTTGCTGCATTGCGCCGTCAACTTACGATGCTGAAAGATATGGGTTGTGATGCCATCCGCACCGCTCACAATATGCCGGCACCGGAGTTAGTGCAACTTTGTGATGAAATGGGTTTTATGATGATGCTGGAACCTTTCGACGAATGGGACATTGCCAAATGTGAGAATGGCTATCACCGTTATTTCAACGAGTGGGCAGAACGTGATATGATAAATATGTTGCATCAGTTCCGCAACAATCCTTGTGTCGTAATGTGGAGTATCGGTAATGAAGTTCCTACCCAATGTAGTCCCGTAGGCTATAAAGTCGCTTCTTTCTTGCAGGATATCTGTCATCGTGAAGATCCGACACGTCCTGTTACTTGCGGCATGGATCAGGTGACTTGTGTTCTTGCTAATGGTTTTGCCGCCATGATTGATGTGCCCGGTTTTAATTATCGCGCACACCGTTATCTGGAAGCTTATGAACTGTTGCCGCAGAATATAGTACTTGGTTCTGAAACATCCTCTACCGTTAGTTCTCGTGGCGTATATAAATTTCCTGTAGAGAAACGCGGGGATGCGAAGTACGATGATCACCAGTCTTCCGGATATGACTTGGAGCATTGTGCCTGGTCTAATGTTCCAGATGAAGATTTTGCTTTAGCGGATGATTATGACTGGACTATCGGTCAATTCGTTTGGACAGGATTCGATTATCTGGGTGAGCCTTCTCCTTATGATACGGATGCATGGCCAAGTCATAGCTCTTTGTTTGGTATCATTGACCTTGCCAGTTTGCCAAAAGACCGCTACTATCTGTACCGTAGTCTTTGGAATAAGAATGTGAATACACTCCATATACTTCCTCACTGGACATGGCCGGGTAGGGAAGGAGAGAATACTCCTGTCTTTGTTTACACAAACTATCCTGCTGCCGAACTTTTCGTTAATGGAAAAAGCTATGGTAAACAGCATAAACTGACAGCCGAAGAGAGTAAAGCTATTCAGGACAAAGATACACTTGCCCTCCAGCGTCGTTACCGCCTGATGTGGATGGACGTTCCTTATGAGCCGGGTGAAGTGAAAGTGGTGGCTTACGATGCTTCCGGCAAACCTGCTGAAGAAAAAGTAGTTCGTACTTCCGGCAAACCTCATCATCTGGAAGTCATTGCTGACCGTGACCAACTCACTGCCGATGGTAAAGATTTGGCATACATCACTGTTCGTGTGGTTGATAAAGACGGAAACCTTTGTCCTGCTGATAATCGTCTTGTAAACTTTACGGTGAAAGGCGCGGGGCGTTATCGTGCTGCCGCTAATGGAGATGCAACTTCACTTGATTTATTCCACTTGCCGAAGATGCCCGCTTTCAGTGGTCAGCTGACAGCCATTGTTCAAATGACCGAACAGCCCGGTGAAATTATTTTCGAGGCTAAGGCTAAAGGGGTGAAATCTGGTAAGCTTGTGCTGAGGTCTGTTAGAGAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,538 total letters Query= CMR200027.125 Length=2820 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4351 0.0 2_H3_CMR200027.125Seq171H09.seq 1797 0.0 1_03H_CMR200027.125Seq171H03.seq 1642 0.0 I V S G * Q F P S R N N F V * L * E G D I H M H H H H H H S Query_1 40 ATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCT 129 0 1020 ........... 1010 S G V D L W S H P Q F E K G T E N L Y F Q S N A Q R S E Y L Query_1 130 TCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAGCGCAGTGAGTATCTA 219 0 1 .................. 18 L E K N W K F M K G E A P E A M K P E F D D R K W E T V T V Query_1 220 CTTGAAAAGAACTGGAAGTTCATGAAGGGGGAAGCTCCGGAAGCCATGAAGCCGGAATTTGACGACCGGAAGTGGGAAACCGTAACCGTG 309 0 19 .......................................................................................... 108 P H D W A I F G P F D R S N D L Q E V A V T Q N F E K K A S Query_1 310 CCTCACGACTGGGCCATTTTTGGTCCCTTCGATCGCAGCAACGATTTGCAGGAAGTGGCGGTAACGCAGAACTTCGAGAAGAAAGCTTCC 399 0 109 .......................................................................................... 198 V K T G R T G G L P Y V G I G W Y R T R F D A P V N Q Q T T Query_1 400 GTCAAGACCGGACGTACCGGTGGACTTCCTTATGTTGGCATCGGATGGTATCGTACTAGGTTCGATGCCCCCGTCAATCAACAGACGACA 489 0 199 .......................................................................................... 288 L V F D G A M S E A R V Y V N G Q E A C F W P F G Y N S F Query_1 490 CTTGTCTTTGATGGTGCCATGAGCGAAGCCCGTGTATATGTCAATGGACAAGAAGCATGCTTC-TGGCCATTTGGTTATAATTCTTTCCA 578 0 289 ...............................................................-.......................... 377 0 359 .....C..-............T..T..... 331 H C D V T G L L N K D G K N N T L A V R L E N K P Q S S R W Query_1 579 TTGTGATGTCACCGGACTTTTGAATAAAGACGGTAAAAACAATACGCTTGCCGTGCGTTTGGAAAATAAACCA-CAATCTTCCCGTTGGT 667 0 378 .........................................................................-................ 466 2 1109 ..G.......T....NN................ 1077 Y P G A G L Y R N V R V V S T D K V H V P V W G T Q L Query_1 668 AT-CCTGG-CGCAGGACTTTATCGCAATGTGC-GTGTAGTG-AGTACCG-AT-AAAGTACATGTT-CCTGTATGGGG-TACT-CAG-CTG 747 0 467 ..-.....-.......................-........-.......-..-............-...........-....-...-... 546 2 1076 ..T.....AN.....N...N-.....NN....N.N......G..GNT..N..N....N.......T..N........G....T...G... 988 T T P H V S D E Y A S V R L L T T I A N D E E R D I R I Query_1 748 ACTACTCCTCATGTTT-CTGATG-AGTATGC-TTCAGTACGTCTGTTGACCACTATTGCCAATGATGAAGAAA-GAGATATCCGTATCGT 833 0 547 ................-......-.......-.........................................-................ 632 2 987 ................T......G......NN.........................................A................ 898 V T E I I S P D G K V V A T K D N T R K I N H G Q P F E Q N Query_1 834 GACAGAGATAATCTCTCCCGATGGGAAAGTCGTTGCAACGAAGGATAATACCCGTAAGATTAATCATGGTCAGCCTTTTGAACAAAACTT 923 0 633 .......................................................................................... 722 0 673 ............ 662 2 897 .......................................................................................... 808 2 857 ............ 868 F L V N A P C L W S P E T P Y L Y K A V S K I Y A D G K Q T Query_1 924 CCTGGTGAATGCTCCTTGCTTGTGGTCGCCGGAGACACCTTATTTATATAAAGCTGTTTCTAAAATCTATGCCGATGGCAAGCAAACGGA 1013 0 723 .......................................................................................... 812 2 807 .-........................................................................................ 719 D E Y T T R F G I R S I E I I A D K G F F L N G K H R K F Q Query_1 1014 TGAATACACTACTCGTTTCGGCATCCGCAGCATAGAAATCATTGCCGACAAAGGATTTTTCCTGAACGGTAAGCATCGCAAGTTCCAGGG 1103 0 813 .......................................................................................... 902 2 718 .......................................................................................... 629 G V C N H H D L G P L G A A I N V A A L R R Q L T M L K D M Query_1 1104 GGTGTGCAATCACCACGATCTTGGTCCGTTAGGCGCTGCCATCAATGTTGCTGCATTGCGCCGTCAACTTACGATGCTGAAAGATATGGG 1193 0 903 .......................................................................................... 992 2 628 .......................................................................................... 539 G C D A I R T A H N M P A P E L V Q L C D E M G F M M M L E Query_1 1194 TTGTGATGCCATCCGCACCGCTCACAATATGCCGGCACCGGAGTTAGTGCAACTTTGTGATGAAATGGGTTTTATGATGATGCTGGAACC 1283 0 993 .......................................................................................... 1082 2 538 .......................................................................................... 449 P F D E W D I A K C E N G Y H R Y F N E W A E R D M I N M L Query_1 1284 TTTCGACGAATGGGACATTGCCAAATGTGAGAATGGCTATCACCGTTATTTCAACGAGTGGGCAGAACGTGATATGATAAATATGTTGCA 1373 0 1083 .......................................................................................... 1172 2 448 .......................................................................................... 359 1 1 ........-.................................... 44 H Q F R N N P C V V M W S I G N E V P T Q C S P V G Y K V A Query_1 1374 TCAGTTCCGCAACAATCCTTGTGTCGTAATGTGGAGTATCGGTAATGAAGTTCCTACCCAATGTAGTCCCGTAGGCTATAAAGTCGCTTC 1463 0 1173 .......................................................................................... 1262 2 358 .......................................................................................... 269 1 45 .......................................................................................... 134 S F L Q D I C H R E D P T R P V T C G M D Q V T C V L A N G Query_1 1464 TTTCTTGCAGGATATCTGTCATCGTGAAGATCCGACACGTCCTGTTACTTGCGGCATGGATCAGGTGACTTGTGTTCTTGCTAATGGTTT 1553 0 1263 .......................................................................................... 1352 2 268 .......................................................................................... 179 1 135 .......................................................................................... 224 F A A M I D V P G F N F R A H R Y L E A Y E L L P Q N I V L Query_1 1554 TGCCGCCATGATTGATGTGCCCGGTTTTAATTTTCGCGCACACCGTTATCTGGAAGCTTATGAACTGTTGCCGCAGAATATAGTACTTGG 1643 0 1353 ................................A......................................................... 1442 2 178 .......................................................................................... 89 1 225 .......................................................................................... 314 G S E T S S T V S S R G V Y K F P V E K R G D A K Y D D H Q Query_1 1644 TTCTGAAACATCCTCTACCGTTAGTTCTCGTGGCGTATATAAATTTCCTGTAGAGAAACGCGGGGATGCGAAGTACGATGATCACCAGTC 1733 0 1443 .......................................................................................... 1532 2 88 ..............................................................................-N........ 2 1 315 .......................................................................................... 404 S S G Y D L E H C A W S N V P D E D F A L A D D Y D W T I G Query_1 1734 TTCCGGATATGACTTGGAGCATTGTGCCTGGTCTAATGTTCCAGATGAAGATTTTGCTTTAGCGGATGATTATGACTGGACTATCGGTCA 1823 0 1533 .......................................................................................... 1622 1 405 .......................................................................................... 494 Q F V W T G F D Y L G E P S P Y D T D A W P S H S S L F G I Query_1 1824 ATTCGTTTGGACAGGATTCGATTATCTGGGTGAGCCTTCTCCTTATGATACGGATGCATGGCCAAGTCATAGCTCTTTGTTTGGTATCAT 1913 0 1623 .......................................................................................... 1712 1 495 .......................................................................................... 584 I D L A S L P K D R Y Y L Y R S L W N K N V N T L H I L P H Query_1 1914 TGACCTTGCCAGTTTGCCAAAAGACCGCTACTATCTGTACCGTAGTC-TTTGGAATAAGAATGTGAATACACTCCATATACTTCCTCACT 2002 0 1713 ...............................................-.......................................... 1801 1 585 ............................................N..T.......................................... 674 W T W P G R E G E N T P V F V Y T N Y P A A E L F V N G K S Query_1 2003 GGACATGGCCGGGTAGGGAAGGAGAGAATACTCCTGTCTTTGTTTACACAAACTATCCTGCTGCCGAACTTTTCGTTAATGGAAAAAGCT 2092 0 1802 .......................................................................................... 1891 1 675 .......................................................................................... 764 Y G K Q H K L T A E E S K A I Q D K D T L A L Q R R Y R L Query_1 2093 ATGGTAAACAGCATAAACTGACAG-CCGAAGAGAGTAAAGCTATTCAGGAC-AAAGATACACTTGCCCTCCAGCGTCGTTACCGCCTGAT 2180 0 1892 ........................-..........................-...................................... 1979 1 765 ........................C..........................A...................................... 854 M W M D V P Y E P G E V K V V A Y D A S G K P A E E K V V R Query_1 2181 GTGGATGGACGTTCCTTATGAGCCGGGTGAAGTGAAAGTGGTGGCTTACGATGCTTCCGGCAAACCTGCTGAAGAAAAAGTAGTTCGTAC 2270 0 1980 .......................................................................................... 2069 0 970 ........... 980 1 855 ..............T................N.............C......................N..-.T.....A.......... 943 T S G K P H H L E V I A D R D Q L T A D G K D L A Y I T V R Query_1 2271 -TTCCGGCAAACCTCATCATCTGGAAGTCATTGCTGACCGTGACCAACTCACTGCCGATGGTAAAGATTTGGCATACATCACTGTTCGTG 2359 0 2070 -......................................................................................... 2158 1 944 T......T..C.T..N.NT... 965 V V D K D G N L C P A D N R L V N F T V K G A G R Y R A A A Query_1 2360 TGGTTGATAAAGACGGAAACCTTTGTCCTGCTGATAATCGTCTTGTAAACTTTACGGTGAAAGGCGCGGGGCGTTATCGTGCTGCCGCTA 2449 0 2159 .......................................................................................... 2248 N G D A T S L D L F H L P K M P A F S G Q L T A I V Q M T E Query_1 2450 ATGGAGATGCAACTTCACTTGATTTATTCCACTTGCCGAAGATGCCCGCTTTCAGTGGTCAGCTGACAGCCATTGTTCAAATGACCGAAC 2539 0 2249 .......................................................................................... 2338 Q P G E I I F E A K A K G V K S G K L V L R S V R E Query_1 2540 AGCCCGGTGAAATTATTTTCGAGGCTAAGGCTAAAGGGGTGAAATCTGGTAAGCTTGTGCTGAGGTCTGTTAGAGAG 2616 0 2339 ............................................................................. 2415 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12577061 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,538 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200052.104 (CMR200052.24-704.P(704).E419A.pMCSG68)
REVTPFNNGWEFKKGPFPTDAMQTAARWNADWEQVNVPHTWNADDMQKKTNSFYAGVAYYRKHYVFPESLEGKRLFLRFEGVGACAEVYVNGYLVGTHKGAYSAFVCEIGSQVRLGEENEIVVKSDNVARPDVIPVNHALFGVYGGIYRPVWLIVTEPCNIVVNDCASPGVYITQKNVSKQSADVSVRVKLDNATLAPAALELENAIYTREGKLVKVHRQFFELTPQGVQNYVSDFKISHPHLWQGREDPYLYKVVSRLKQDGQVIDEVIQPLGLRKYEAVAGKGFFLNGKKYPMYGVTRHQDWWGMGSALTNREHDFDLEQIMEVGATTVRFAHYQQSDYIYSRCDTLGLIIWAEIPFVNRVTGQEWDNVHQQMRELIRQSFNHPSIYVWGIHNEVYHPHGYTAALTQSVHDLCKLEDPDRYTVAVNGYGHAEHPVNGNTDIQGMNRYFGWYEKKIQDIKPWIEKMAKEYPWQRLMLTEYGADANIEHQTELLGDALNWGSDFYPETFQTKTHEYQWGVISQHPYILASYLWNMFDFAVPQWKRGGVDARNMKGLITFDRKIRKDAFYWYKANWSKEPVLYLTQRRNTERERRETSVTVYSNLGTPRVFLNGRELDGVRKGYTDVHYIFDQVTLAEERNVLRAVIVSDGKEYTDEIVWNYNGEHVRQADAYEHKKEHAGW
CGTGAGGTAACTCCATTCAATAACGGATGGGAGTTTAAGAAAGGGCCTTTTCCAACCGATGCGATGCAGACTGCTGCCAGATGGAATGCAGATTGGGAACAGGTGAATGTGCCTCATACCTGGAATGCGGATGATATGCAGAAGAAAACCAACAGCTTTTATGCTGGGGTAGCTTATTATCGGAAGCACTATGTTTTTCCTGAATCACTGGAAGGCAAGCGTCTTTTTCTACGCTTTGAGGGTGTAGGAGCATGTGCCGAAGTTTATGTCAACGGTTATTTGGTTGGAACACATAAAGGAGCTTATTCTGCATTTGTCTGCGAAATTGGTTCGCAAGTCAGGCTGGGAGAAGAGAATGAAATTGTTGTCAAGTCCGATAATGTAGCACGTCCGGATGTGATTCCGGTCAACCATGCTCTTTTTGGCGTCTATGGTGGAATTTACCGTCCCGTATGGCTGATAGTGACTGAACCCTGCAATATTGTAGTGAATGACTGTGCCTCTCCGGGTGTATATATAACTCAGAAGAACGTATCGAAACAGTCTGCTGATGTTTCTGTCAGGGTGAAACTTGATAATGCAACATTGGCTCCGGCTGCTCTTGAACTGGAAAATGCGATTTATACCCGTGAGGGCAAGTTGGTTAAAGTTCATCGGCAGTTTTTTGAGCTGACTCCTCAGGGAGTACAAAACTATGTTTCTGATTTTAAAATCAGTCATCCTCATCTTTGGCAGGGGCGCGAAGATCCTTATTTATATAAGGTCGTCTCTCGTCTGAAACAGGACGGACAGGTAATTGATGAAGTGATACAGCCACTCGGCTTGCGTAAGTATGAGGCTGTGGCTGGAAAAGGTTTCTTCCTGAATGGTAAAAAATATCCGATGTATGGAGTTACCCGTCATCAGGACTGGTGGGGGATGGGTAGCGCACTGACTAACAGGGAACATGATTTTGATCTGGAGCAGATTATGGAAGTTGGGGCAACGACCGTGCGTTTTGCTCATTACCAGCAGTCGGATTATATTTATTCACGTTGTGATACGTTGGGACTTATTATCTGGGCTGAGATTCCTTTTGTGAACCGTGTGACAGGGCAGGAGTGGGATAATGTTCATCAGCAAATGCGCGAGCTGATACGTCAGAGTTTCAATCATCCTTCTATTTATGTGTGGGGAATTCATAACGAGGTGTATCATCCGCATGGTTATACGGCTGCTCTTACTCAGTCTGTTCACGATCTCTGCAAACTGGAAGATCCTGATCGTTATACGGTGGCTGTCAATGGTTATGGCCATGCAGAACATCCGGTTAACGGGAATACCGATATACAGGGAATGAACCGTTATTTCGGATGGTATGAGAAAAAGATACAGGATATCAAACCCTGGATTGAAAAAATGGCAAAAGAATATCCCTGGCAACGGCTGATGCTGACAGAATATGGGGCTGATGCGAACATTGAGCATCAGACAGAATTGCTGGGAGATGCCTTGAACTGGGGCAGTGATTTTTATCCGGAAACTTTCCAGACAAAGACACATGAGTATCAGTGGGGAGTAATTTCTCAGCATCCTTATATTCTTGCTTCTTATTTATGGAATATGTTTGACTTTGCAGTTCCTCAATGGAAGCGTGGAGGTGTAGATGCGCGGAACATGAAAGGGTTGATAACTTTTGATCGTAAAATACGCAAGGATGCTTTCTATTGGTATAAGGCAAACTGGAGTAAGGAGCCGGTGCTTTATCTGACGCAACGCCGTAATACGGAACGTGAACGCCGGGAAACTTCTGTCACTGTCTATTCCAATCTTGGGACACCCCGCGTTTTCCTGAATGGCCGGGAACTGGATGGCGTTCGTAAGGGATATACCGATGTGCATTACATTTTTGATCAGGTAACGCTTGCCGAAGAACGAAATGTACTTAGAGCTGTTATCGTGTCTGATGGTAAGGAATATACCGATGAAATTGTGTGGAACTACAACGGTGAGCATGTCCGGCAGGCCGATGCTTACGAACATAAGAAAGAACATGCCGGCTGG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,534 total letters Query= CMR200052.104 Length=2448 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3676 0.0 1_02H_H5_CMR200052.104_T7term.seq 1831 0.0 2_05H_H5_CMR200052.104_T7S.seq 1819 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 58 TTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGG 147 2 11 .........N...-............................................---........A.................... 96 S H P Q F E K G T E N L Y F Q S N A R E V T P F N N G W E F Query_1 148 TCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCGTGAGGTAACTCCATTCAATAACGGATGGGAGTTT 237 0 1 .................................... 36 0 897 ...........A.. 884 2 97 .................................G........................................................ 186 K K G P F P T D A M Q T A A R W N A D W E Q V N V P H T W N Query_1 238 AAGAAAGGGCCTTTTCCAACCGATGCGATGCAGACTGCTGCCAGATGGAATGCAGATTGGGAACAGGTGAATGTGCCTCATACCTGGAAT 327 0 37 .......................................................................................... 126 0 1203 .. 1202 1 974 .. 975 2 187 .......................................................................................... 276 A D D M Q K K T N S F Y A G V A Y Y R K H Y V F P E S L E G Query_1 328 GCGGATGATATGCAGAAGAAAACCAACAGCTTTTATGCTGGGGTAGCTTATTATCGGAAGCACTATGTTTTTCCTGAATCACTGGAAGGC 417 0 127 .......................................................................................... 216 0 1201 .......... 1192 0 1826 ........... 1836 1 976 .......... 985 1 349 ........... 339 2 277 .......................................................................................... 366 K R L F L R F E G V G A C A E V Y V N G Y L V G T H K G A Y Query_1 418 AAGCGTCTTTTTCTACGCTTTGAGGGTGTAGGAGCATGTGCCGAAGTTTATGTCAACGGTTATTTGGTTGGAACACATAAAGGAGCTTAT 507 0 217 .......................................................................................... 306 2 367 .....................N.................................................................... 456 S A F V C E I G S Q V R L G E E N E I V V K S D N V A R P D Query_1 508 TCTGCATTTGTCTGCGAAATTGGTTCGCAAGTCAGGCTGGGAGAAGAGAATGAAATTGTTGTCAAGTCCGATAATGTAGCACGTCCGGAT 597 0 307 .......................................................................................... 396 2 457 ................................T......................................................... 546 V I P V N H A L F G V Y G G I Y R P V W L I V T E P C N I V Query_1 598 GTGATTCCGGTCAACCATGCTCTTTTTGGCGTCTATGGTGGAATTTACCGTCCCGTATGGCTGATAGTGACTGAACCCTGCAATATTGTA 687 0 397 .......................................................................................... 486 2 547 .......................................................................................... 636 V N D C A S P G V Y I T Q K N V S K Q S A D V S V R V K L D Query_1 688 GTGAATGACTGTGCCTCTCCGGGTGTATATATAACTCAGAAGAACGTATCGAAACAGTCTGCTGATGTTTCTGTCAGGGTGAAACTTGAT 777 0 487 .......................................................................................... 576 2 637 .......................................................................................... 726 N A T L A P A A L E L E N A I Y T R E G K L V K V H R Q F F Query_1 778 AATGCAACATTGGCTCCGGCTGCTCTTGAACTGGAAAATGCGATTTATACCCGTGAGGGCAAGTTGGTTAAAGTTCATCGGCAGTTTTTT 867 0 577 .......................................................................................... 666 0 1211 ........... 1221 1 966 ........... 956 2 727 .......................................................................................... 816 E L T P Q G V Q N Y V S D F K I S H P H L W Q G R E D P Y Query_1 868 GAGCTGACTCCTCAGGGAGTACAAAACTATGTTTCTGATTTTAAAATCAGTCATCCTCA-TCTTTGGCAGGGGCGCGAAGATCCTTATTT 956 0 667 ...........................................................-.............................. 755 0 716 ................ 701 2 817 ......................................-...N................T......................N....... 905 L Y K V V S R L K Q D G Q V I D E V I Q P L G L R K Y E A Query_1 957 ATATAAGGTCGTCT-CTCGTCTGAAACAGGACGGACAGGTAATTGATGAAGTGATACAGCCA-CTCGGCTTGCGTAAGTATG-AGGCTGT 1043 0 756 ..............-...............................................-...................-....... 842 2 906 ..N...........C.....N...........C.......-.....................C...................A....... 994 V A G K G F F L N G K K Y P M Y G V T R H Q D W W G M G S Query_1 1044 GGCTGGAAAAGG-TTTCTTCCTGAATGGTAAAAAATATCCGATGTATGGAGTTAC-CCGTCAT-CAGGAC-TGGTGGGG-GATGGGTAGC 1128 0 843 ............-..........................................-.......-......-........-.......... 927 0 19 ..A..........-. 6 0 1828 ........... 1838 1 347 ........... 337 2 995 ............T.....................TAT.......CN.........T.......N......T........NN....NN... 1084 2 169 ..A..........-. 156 A L T N R E H D F D L E Q I M E V G A T T V R F A H Y Query_1 1129 GCACTGACTAACAGGGAA-CATGATTTTGATCTGGAGCAGATT--ATGG-AA--GTTGGG-GCAACGACCGTGCGTTTTGCTCATTACCA 1211 0 928 ..................-........................--....-..--......-............................. 1010 1 1239 ........NN....-N.AG.....A-...NA.NNN...GCN....N..CA.NN-. 1188 2 1085 ...............N..C.....N......NNN.NN..A...--....N..--......TNN...... 1149 Q Q S D Y I Y S R C D T L G L I I W A E I P F V N R V T Query_1 1212 GCAGT-CGGATTATATTTA--TTCAC-GTTGTG-ATACGTTGGGACTTATTAT-CTGGGCTGAGATTCCTTTTGTGAACC-GTGTGACAG 1294 0 1011 .....-.............--.....-......-...................-..........................-......... 1093 1 1187 .....T.........ANNNAT.....C.N.N..N..N........N.AT....T...NNNGA.....N.C.N.N......N....N...N 1098 G Q E W D N V H Q Q M R E L I R Q S F N H P S I Y V Query_1 1295 GGCA--GGAG-TGGGA-TAA-TGTT-CATCAGCAAAT-GCGC-GAG-CTGATACGT-CAGAGTTTCAATCATCCTTCTATTTA-TGTGTG 1373 0 1094 ....--....-.....-...-....-...........-....-...-.........-..........................-...... 1172 1 1097 ...CAG....G.....A...N....T...........T....N...G.........T.....N..............NN....N...N.. 1008 W G I H N A V Y H P H G Y T A A L T Q S V H D L C K L E D Query_1 1374 GGGAA--TTCA-TAACGCTGTGTATCATCCGCATGGTTATACGGCTGCTCTTACTCAGTCT-GTTCACGATCTCTGCAAACTGGAAGATC 1459 0 1173 .....--....-.....AG..........................................-............................ 1258 0 136 ............ 125 0 593 ........... 603 1 1007 ...G.AT....A........................G........................A............................ 918 2 286 ............ 275 2 743 ........... 753 P D R Y T V A V N G Y G H A E H P V N G N T D I Q G M N R Y Query_1 1460 CTGATCGTTATACGGTGGCTGTCAATGGTTATGGCCATGCAGAACATCCGGTTAACGGGAATACCGATATACAGGGAATGAACCGTTATT 1549 0 1259 .......................................................................................... 1348 1 917 .......................................................................................... 828 F G W Y E K K I Q D I K P W I E K M A K E Y P W Q R L M L T Query_1 1550 TCGGATGGTATGAGAAAAAGATACAGGATATCAAACCCTGGATTGAAAAAA-TGGCAAAAGAATATCCCTGGCAACGGCTGATGCTGACA 1638 0 1349 ...................................................-...................................... 1437 1 827 ...................................................A...................................... 738 E Y G A D A N I E H Q T E L L G D A L N W G S D F Y P E T F Query_1 1639 GAATATGGGGCTGATGCGAACATTGAGCATCAGACAGAATTGCTGGGAGATGCCTTGAACTGGGGCAGTGATTTTTATCCGGAAACTTTC 1728 0 1438 .......................................................................................... 1527 1 737 .......................................................................................... 648 Q T K T H E Y Q W G V I S Q H P Y I L A S Y L W N M F D F A Query_1 1729 CAGACAAAGACACATGAGTATCAGTGGGGAGTAATTTCTCAGCATCCTTATATTCTTGCTTCTTATTTATGGAATATGTTTGACTTTGCA 1818 0 1528 .......................................................................................... 1617 1 647 .......................................................................................... 558 V P Q W K R G G V D A R N M K G L I T F D R K I R K D A F Y Query_1 1819 GTTCCTCAATGGAAGCGTGGAGGTGTAGATGCGCGGAACATGAAAGGGTTGATAACTTTTGATCGTAAAATACGCAAGGATGCTTTCTAT 1908 0 1618 .......................................................................................... 1707 1 557 .......................................................................................... 468 W Y K A N W S K E P V L Y L T Q R R N T E R E R R E T S V T Query_1 1909 TGGTATAAGGCAAACTGGAGTAAGGAGCCGGTGCTTTATCTGACGCAACGCCGTAATACGGAACGTGAACGCCGGGAAACTTCTGTCACT 1998 0 1708 .......................................................................................... 1797 1 467 .......................................................................................... 378 V Y S N L G T P R V F L N G R E L D G V R K G Y T D V H Y I Query_1 1999 GTCTATTCCAATCTTGGGACACCCCGCGTTTTCCTGAATGGCCGGGAACTGGATGGCGTTCGTAAGGGATATACCGATGTGCATTACATT 2088 0 1798 .......................................................................................... 1887 0 1949 ......A........... 1966 0 195 ........... 205 0 859 ........... 869 1 377 .......................................................................................... 288 1 226 ......A........... 209 2 345 ........... 355 2 1012 ........... 1022 F D Q V T L A E E R N V L R A V I V S D G K E Y T D E I V W Query_1 2089 TTTGATCAGGTAACGCTTGCCGAAGAACGAAATGTACTTAGAGCTGTTATCGTGTCTGATGGTAAGGAATATACCGATGAAATTGTGTGG 2178 0 1888 .......................................................................................... 1977 0 1859 ......G........... 1876 1 287 .......................................................................................... 198 1 316 ......G........... 299 N Y N G E H V R Q A D A Y E H K K E H A G W * H W K W I T D Query_1 2179 AACTACAACGGTGAGCATGTCCGGCAGGCCGATGCTTACGAACATAAGAAAGAACATGCCGGCTGGTAACATTGGAAGTGGATAACGGAT 2268 0 1978 .................................................................. 2043 1 197 .......................................................................................... 108 P N S S S V D K L A A A L E H H H H H H * D P A A N K A R Query_1 2269 CCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAA 2357 1 107 ......................................................................................... 19 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 10898173 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,534 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200052.105 (CMR200052.24-704.P(704).E503A.pMCSG68)
REVTPFNNGWEFKKGPFPTDAMQTAARWNADWEQVNVPHTWNADDMQKKTNSFYAGVAYYRKHYVFPESLEGKRLFLRFEGVGACAEVYVNGYLVGTHKGAYSAFVCEIGSQVRLGEENEIVVKSDNVARPDVIPVNHALFGVYGGIYRPVWLIVTEPCNIVVNDCASPGVYITQKNVSKQSADVSVRVKLDNATLAPAALELENAIYTREGKLVKVHRQFFELTPQGVQNYVSDFKISHPHLWQGREDPYLYKVVSRLKQDGQVIDEVIQPLGLRKYEAVAGKGFFLNGKKYPMYGVTRHQDWWGMGSALTNREHDFDLEQIMEVGATTVRFAHYQQSDYIYSRCDTLGLIIWAEIPFVNRVTGQEWDNVHQQMRELIRQSFNHPSIYVWGIHNEVYHPHGYTAALTQSVHDLCKLEDPDRYTVAVNGYGHAEHPVNGNTDIQGMNRYFGWYEKKIQDIKPWIEKMAKEYPWQRLMLTEYGADANIEHQTELLGDALNWGSDFYPETFQTKTHEYQWGVISQHPYILASYLWNMFDFAVPQWKRGGVDARNMKGLITFDRKIRKDAFYWYKANWSKEPVLYLTQRRNTERERRETSVTVYSNLGTPRVFLNGRELDGVRKGYTDVHYIFDQVTLAEERNVLRAVIVSDGKEYTDEIVWNYNGEHVRQADAYEHKKEHAGW
CGTGAGGTAACTCCATTCAATAACGGATGGGAGTTTAAGAAAGGGCCTTTTCCAACCGATGCGATGCAGACTGCTGCCAGATGGAATGCAGATTGGGAACAGGTGAATGTGCCTCATACCTGGAATGCGGATGATATGCAGAAGAAAACCAACAGCTTTTATGCTGGGGTAGCTTATTATCGGAAGCACTATGTTTTTCCTGAATCACTGGAAGGCAAGCGTCTTTTTCTACGCTTTGAGGGTGTAGGAGCATGTGCCGAAGTTTATGTCAACGGTTATTTGGTTGGAACACATAAAGGAGCTTATTCTGCATTTGTCTGCGAAATTGGTTCGCAAGTCAGGCTGGGAGAAGAGAATGAAATTGTTGTCAAGTCCGATAATGTAGCACGTCCGGATGTGATTCCGGTCAACCATGCTCTTTTTGGCGTCTATGGTGGAATTTACCGTCCCGTATGGCTGATAGTGACTGAACCCTGCAATATTGTAGTGAATGACTGTGCCTCTCCGGGTGTATATATAACTCAGAAGAACGTATCGAAACAGTCTGCTGATGTTTCTGTCAGGGTGAAACTTGATAATGCAACATTGGCTCCGGCTGCTCTTGAACTGGAAAATGCGATTTATACCCGTGAGGGCAAGTTGGTTAAAGTTCATCGGCAGTTTTTTGAGCTGACTCCTCAGGGAGTACAAAACTATGTTTCTGATTTTAAAATCAGTCATCCTCATCTTTGGCAGGGGCGCGAAGATCCTTATTTATATAAGGTCGTCTCTCGTCTGAAACAGGACGGACAGGTAATTGATGAAGTGATACAGCCACTCGGCTTGCGTAAGTATGAGGCTGTGGCTGGAAAAGGTTTCTTCCTGAATGGTAAAAAATATCCGATGTATGGAGTTACCCGTCATCAGGACTGGTGGGGGATGGGTAGCGCACTGACTAACAGGGAACATGATTTTGATCTGGAGCAGATTATGGAAGTTGGGGCAACGACCGTGCGTTTTGCTCATTACCAGCAGTCGGATTATATTTATTCACGTTGTGATACGTTGGGACTTATTATCTGGGCTGAGATTCCTTTTGTGAACCGTGTGACAGGGCAGGAGTGGGATAATGTTCATCAGCAAATGCGCGAGCTGATACGTCAGAGTTTCAATCATCCTTCTATTTATGTGTGGGGAATTCATAACGAGGTGTATCATCCGCATGGTTATACGGCTGCTCTTACTCAGTCTGTTCACGATCTCTGCAAACTGGAAGATCCTGATCGTTATACGGTGGCTGTCAATGGTTATGGCCATGCAGAACATCCGGTTAACGGGAATACCGATATACAGGGAATGAACCGTTATTTCGGATGGTATGAGAAAAAGATACAGGATATCAAACCCTGGATTGAAAAAATGGCAAAAGAATATCCCTGGCAACGGCTGATGCTGACAGAATATGGGGCTGATGCGAACATTGAGCATCAGACAGAATTGCTGGGAGATGCCTTGAACTGGGGCAGTGATTTTTATCCGGAAACTTTCCAGACAAAGACACATGAGTATCAGTGGGGAGTAATTTCTCAGCATCCTTATATTCTTGCTTCTTATTTATGGAATATGTTTGACTTTGCAGTTCCTCAATGGAAGCGTGGAGGTGTAGATGCGCGGAACATGAAAGGGTTGATAACTTTTGATCGTAAAATACGCAAGGATGCTTTCTATTGGTATAAGGCAAACTGGAGTAAGGAGCCGGTGCTTTATCTGACGCAACGCCGTAATACGGAACGTGAACGCCGGGAAACTTCTGTCACTGTCTATTCCAATCTTGGGACACCCCGCGTTTTCCTGAATGGCCGGGAACTGGATGGCGTTCGTAAGGGATATACCGATGTGCATTACATTTTTGATCAGGTAACGCTTGCCGAAGAACGAAATGTACTTAGAGCTGTTATCGTGTCTGATGGTAAGGAATATACCGATGAAATTGTGTGGAACTACAACGGTGAGCATGTCCGGCAGGCCGATGCTTACGAACATAAGAAAGAACATGCCGGCTGG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,422 total letters Query= CMR200052.105 Length=2448 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3676 0.0 2_06A_A6_CMR200052.105_T7S.seq 1678 0.0 1_03A_A6_CMR200052.105_T7term.seq 1647 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W S Query_1 61 CCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCT 150 2 12 .....NN...N-...........................................---................................ 97 H P Q F E K G T E N L Y F Q S N A R E V T P F N N G W E F K Query_1 151 CATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCGTGAGGTAACTCCATTCAATAACGGATGGGAGTTTAAG 240 0 1 ....................................... 39 0 897 ...........A.. 884 2 98 .......................................................................................... 187 K G P F P T D A M Q T A A R W N A D W E Q V N V P H T W N A Query_1 241 AAAGGGCCTTTTCCAACCGATGCGATGCAGACTGCTGCCAGATGGAATGCAGATTGGGAACAGGTGAATGTGCCTCATACCTGGAATGCG 330 0 40 .......................................................................................... 129 0 1203 ..... 1199 2 188 .......................................................................................... 277 D D M Q K K T N S F Y A G V A Y Y R K H Y V F P E S L E G K Query_1 331 GATGATATGCAGAAGAAAACCAACAGCTTTTATGCTGGGGTAGCTTATTATCGGAAGCACTATGTTTTTCCTGAATCACTGGAAGGCAAG 420 0 130 .......................................................................................... 219 0 1198 ....... 1192 0 1826 ........... 1836 2 278 .......................................................................................... 367 1 352 ........... 342 R L F L R F E G V G A C A E V Y V N G Y L V G T H K G A Y S Query_1 421 CGTCTTTTTCTACGCTTTGAGGGTGTAGGAGCATGTGCCGAAGTTTATGTCAACGGTTATTTGGTTGGAACACATAAAGGAGCTTATTCT 510 0 220 .......................................................................................... 309 2 368 .......................................................................................... 457 A F V C E I G S Q V R L G E E N E I V V K S D N V A R P D V Query_1 511 GCATTTGTCTGCGAAATTGGTTCGCAAGTCAGGCTGGGAGAAGAGAATGAAATTGTTGTCAAGTCCGATAATGTAGCACGTCCGGATGTG 600 0 310 .......................................................................................... 399 2 458 .......................................................................................... 547 I P V N H A L F G V Y G G I Y R P V W L I V T E P C N I V V Query_1 601 ATTCCGGTCAACCATGCTCTTTTTGGCGTCTATGGTGGAATTTACCGTCCCGTATGGCTGATAGTGACTGAACCCTGCAATATTGTAGTG 690 0 400 .......................................................................................... 489 2 548 .......................................................................................... 637 N D C A S P G V Y I T Q K N V S K Q S A D V S V R V K L D Query_1 691 AATGACTGTGCCTCTCCGGGTGTATATATAACTCAGAAGAACGTATCGAAACAGTCTGCTGAT--GTTTCTGTCAGGGTGAAACTTGATA 778 0 490 ...............................................................--......................... 577 2 638 ...............................................................TGT.................N...... 727 N A T L A P A A L E L E N A I Y T R E G K L V K V H R Q F Query_1 779 ATGCAACATTGGCT-CCGGCTGCTCTTGAACTGGAAAATGCGA-TTTATACCCGTGAGGGCAAGTTGGTTAAAGTTCATCGGCAGTTTTT 866 0 578 ..............-............................-.............................................. 665 0 1211 ........... 1221 2 728 ..............C............................T........................-................-.... 815 F E L T P Q G V Q N Y V S D F K I S H P H L W Q G R E D P Y Query_1 867 TGAGCTGACT-CCTCAGGGAGTACAAAACTA-TGTTTCTGATTTTAAAATCAGTCATCCTCATCTTTGGCAGGGGCGCGAAGATCCTTAT 954 0 666 ..........-....................-.......................................................... 753 0 716 ................ 701 2 816 ..........C....................T.........-...N.....-..............N..NG..............N.... 903 L Y K V V S R L K Q D G Q V I D E V I Q P L G L R K Y E A Query_1 955 TTATATAAGGTCGT-CTCTCGTCTGAAACAGGACGGACAGGTAATTGATGAAGTGATACAGCCACTC-GGCTTGCGTAAGTA-TGAGGCT 1041 0 754 ..............-....................................................-..............-....... 840 2 904 ..............C................T....................N..............T.N..N.........T...A... 993 V A G K G F F L N G K K Y P M Y G V T R H Q D W W G M G S Query_1 1042 GTGGCTGGAAAAGGTTT-CTTCCTGAATGGTAAAAAATATCCGATGTAT-GGAGTTACCCGTCATCAGGACTGGTGGGGGATGGGTAGCG 1129 0 841 .................-...............................-........................................ 928 0 19 ..A..-......... 6 0 1828 ........... 1838 2 994 .................C........TN.CA.............A.GN.N..........NNN....N.....T... 1070 2 167 ..A..-......... 154 1 350 ........... 340 A L T N R E H D F D L E Q I M E V G A T T V R F A H Y Q Q S Query_1 1130 CACTGACTAACAGGGAACATGATTTTGATCTGGAGCAGATTATGGAAGTTGGGGCAACGACCGTGCGTTTTGCTCATTACCAGCAGTCGG 1219 0 929 .......................................................................................... 1018 D Y I Y S R C D T L G L I I W A E I P F V N R V T G Q E W Query_1 1220 ATTATATTTATTCACGTTGTGATACGTTGGGACTTATTATCTGGGCTGAGATTCCTTTTGTGAACCGTGTGACAGGGCAGG-AGTGGG-- 1306 0 1019 .................................................................................-......-- 1105 1 1125 ..........N.N.N..GN 1107 D N V H Q Q M R E L I R Q S F N H P S I Y V W G I Query_1 1307 ATAATG--TTCATCAGCAAATGCGC-GAGC-TGA-TACGTCAG-AGTTT-CAATCATCCT---TCT-ATTTATG-TGT--GGGGAATT-C 1381 0 1106 ......--.................-....-...-........-.....-..........---...-.......-...--........-. 1180 1 1106 .....NNT.........C.......N...NN...N.N...NN.G.....T....TC.T..CTT...TN......N...NG........T. 1017 H N E V Y H P H G Y T A A L T Q S V H D L C K L E D Query_1 1382 ATAAC-GAGGT-GTATC-ATCCGC-ATGGTT--ATACGGC-TGCTC--TTACTCA--GTCTGTTCACGATCTCTGCAAA-CTGGAAGATC 1459 0 1181 .....-.....-.....-......-......--.......-.....--.......--......................-.......... 1258 0 136 ....-......-.. 125 0 593 ....-.....--.. 603 2 284 ....-......-.. 273 2 744 ....-.....--.. 754 1 1016 .....N....NN.....N......N......TAN......N..N.TCT.....NNANN.....................A.......... 927 P D R Y T V A V N G Y G H A E H P V N G N T D I Q G M N R Query_1 1460 CTGATC-GTTATACGGTGGCTGTCAA-TGGTTATGGCCATGCAGAACATCC-GGTTAACGGG-AATACCGATATACAGGGAATGAACCGT 1545 0 1259 ......-...................-........................-..........-........................... 1344 1 926 ......N...................A........................C..........G................N.......... 837 Y F G W Y E K K I Q D I K P W I E K M A K E Y P W Q R L M Query_1 1546 TATTTCGGATGGTATGAGAAAAA-GATACAGGATATCAAACCCTGGATTGAAAAAA-TGGCAAAA-GAATATCCCTGGCAACGGCTGATG 1632 0 1345 .......................-................................-........-........................ 1431 1 836 .......................A................................A........A........................ 747 L T A Y G A D A N I E H Q T E L L G D A L N W G S D F Y P E Query_1 1633 CTGACAGCTTATGGGGCTGATGCGAACATTGAGCATCAGACAGAATTGCTGGGAGATGCCTTGAACTGGGGCAGTGATTTTTATCCGGAA 1722 0 1432 .......AA................................................................................. 1521 1 746 .......................................................................................... 657 T F Q T K T H E Y Q W G V I S Q H P Y I L A S Y L W N M F D Query_1 1723 ACTTTCCAGACAAAGACACATGAGTATCAGTGGGGAGTAATTTCTCAGCATCCTTATATTCTTGCTTCTTATTTATGGAATATGTTTGAC 1812 0 1522 .......................................................................................... 1611 1 656 .......................................................................................... 567 F A V P Q W K R G G V D A R N M K G L I T F D R K I R K D A Query_1 1813 TTTGCAGTTCCTCAATGGAAGCGTGGAGGTGTAGATGCGCGGAACATGAAAGGGTTGATAACTTTTGATCGTAAAATACGCAAGGATGCT 1902 0 1612 .......................................................................................... 1701 1 566 .......................................................................................... 477 F Y W Y K A N W S K E P V L Y L T Q R R N T E R E R R E T S Query_1 1903 TTCTATTGGTATAAGGCAAACTGGAGTAAGGAGCCGGTGCTTTATCTGACGCAACGCCGTAATACGGAACGTGAACGCCGGGAAACTTCT 1992 0 1702 .......................................................................................... 1791 1 476 .......................................................................................... 387 V T V Y S N L G T P R V F L N G R E L D G V R K G Y T D V H Query_1 1993 GTCACTGTCTATTCCAATCTTGGGACACCCCGCGTTTTCCTGAATGGCCGGGAACTGGATGGCGTTCGTAAGGGATATACCGATGTGCAT 2082 0 1792 .......................................................................................... 1881 0 1949 ......A........... 1966 0 195 ........... 205 0 859 ........... 869 2 343 ........... 353 1 386 .......................................................................................... 297 1 229 ......A........... 212 Y I F D Q V T L A E E R N V L R A V I V S D G K E Y T D E I Query_1 2083 TACATTTTTGATCAGGTAACGCTTGCCGAAGAACGAAATGTACTTAGAGCTGTTATCGTGTCTGATGGTAAGGAATATACCGATGAAATT 2172 0 1882 .......................................................................................... 1971 0 1859 ......G........... 1876 1 296 .......................................................................................... 207 1 319 ......G........... 302 V W N Y N G E H V R Q A D A Y E H K K E H A G W * H W K W I Query_1 2173 GTGTGGAACTACAACGGTGAGCATGTCCGGCAGGCCGATGCTTACGAACATAAGAAAGAACATGCCGGCTGGTAACATTGGAAGTGGATA 2262 0 1972 ........................................................................ 2043 1 206 .......................................................................................... 117 T D P N S S S V D K L A A A L E H H H H H H * D P A A N K A Query_1 2263 ACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCC 2352 1 116 .......................................................................................... 27 R K Query_1 2353 CG-AAAGG 2359 1 26 ..N..... 19 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 10625901 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,422 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200052.106 (CMR200052.24-704.P(704).E419Q.pMCSG68)
REVTPFNNGWEFKKGPFPTDAMQTAARWNADWEQVNVPHTWNADDMQKKTNSFYAGVAYYRKHYVFPESLEGKRLFLRFEGVGACAEVYVNGYLVGTHKGAYSAFVCEIGSQVRLGEENEIVVKSDNVARPDVIPVNHALFGVYGGIYRPVWLIVTEPCNIVVNDCASPGVYITQKNVSKQSADVSVRVKLDNATLAPAALELENAIYTREGKLVKVHRQFFELTPQGVQNYVSDFKISHPHLWQGREDPYLYKVVSRLKQDGQVIDEVIQPLGLRKYEAVAGKGFFLNGKKYPMYGVTRHQDWWGMGSALTNREHDFDLEQIMEVGATTVRFAHYQQSDYIYSRCDTLGLIIWAEIPFVNRVTGQEWDNVHQQMRELIRQSFNHPSIYVWGIHNEVYHPHGYTAALTQSVHDLCKLEDPDRYTVAVNGYGHAEHPVNGNTDIQGMNRYFGWYEKKIQDIKPWIEKMAKEYPWQRLMLTEYGADANIEHQTELLGDALNWGSDFYPETFQTKTHEYQWGVISQHPYILASYLWNMFDFAVPQWKRGGVDARNMKGLITFDRKIRKDAFYWYKANWSKEPVLYLTQRRNTERERRETSVTVYSNLGTPRVFLNGRELDGVRKGYTDVHYIFDQVTLAEERNVLRAVIVSDGKEYTDEIVWNYNGEHVRQADAYEHKKEHAGW
CGTGAGGTAACTCCATTCAATAACGGATGGGAGTTTAAGAAAGGGCCTTTTCCAACCGATGCGATGCAGACTGCTGCCAGATGGAATGCAGATTGGGAACAGGTGAATGTGCCTCATACCTGGAATGCGGATGATATGCAGAAGAAAACCAACAGCTTTTATGCTGGGGTAGCTTATTATCGGAAGCACTATGTTTTTCCTGAATCACTGGAAGGCAAGCGTCTTTTTCTACGCTTTGAGGGTGTAGGAGCATGTGCCGAAGTTTATGTCAACGGTTATTTGGTTGGAACACATAAAGGAGCTTATTCTGCATTTGTCTGCGAAATTGGTTCGCAAGTCAGGCTGGGAGAAGAGAATGAAATTGTTGTCAAGTCCGATAATGTAGCACGTCCGGATGTGATTCCGGTCAACCATGCTCTTTTTGGCGTCTATGGTGGAATTTACCGTCCCGTATGGCTGATAGTGACTGAACCCTGCAATATTGTAGTGAATGACTGTGCCTCTCCGGGTGTATATATAACTCAGAAGAACGTATCGAAACAGTCTGCTGATGTTTCTGTCAGGGTGAAACTTGATAATGCAACATTGGCTCCGGCTGCTCTTGAACTGGAAAATGCGATTTATACCCGTGAGGGCAAGTTGGTTAAAGTTCATCGGCAGTTTTTTGAGCTGACTCCTCAGGGAGTACAAAACTATGTTTCTGATTTTAAAATCAGTCATCCTCATCTTTGGCAGGGGCGCGAAGATCCTTATTTATATAAGGTCGTCTCTCGTCTGAAACAGGACGGACAGGTAATTGATGAAGTGATACAGCCACTCGGCTTGCGTAAGTATGAGGCTGTGGCTGGAAAAGGTTTCTTCCTGAATGGTAAAAAATATCCGATGTATGGAGTTACCCGTCATCAGGACTGGTGGGGGATGGGTAGCGCACTGACTAACAGGGAACATGATTTTGATCTGGAGCAGATTATGGAAGTTGGGGCAACGACCGTGCGTTTTGCTCATTACCAGCAGTCGGATTATATTTATTCACGTTGTGATACGTTGGGACTTATTATCTGGGCTGAGATTCCTTTTGTGAACCGTGTGACAGGGCAGGAGTGGGATAATGTTCATCAGCAAATGCGCGAGCTGATACGTCAGAGTTTCAATCATCCTTCTATTTATGTGTGGGGAATTCATAACGAGGTGTATCATCCGCATGGTTATACGGCTGCTCTTACTCAGTCTGTTCACGATCTCTGCAAACTGGAAGATCCTGATCGTTATACGGTGGCTGTCAATGGTTATGGCCATGCAGAACATCCGGTTAACGGGAATACCGATATACAGGGAATGAACCGTTATTTCGGATGGTATGAGAAAAAGATACAGGATATCAAACCCTGGATTGAAAAAATGGCAAAAGAATATCCCTGGCAACGGCTGATGCTGACAGAATATGGGGCTGATGCGAACATTGAGCATCAGACAGAATTGCTGGGAGATGCCTTGAACTGGGGCAGTGATTTTTATCCGGAAACTTTCCAGACAAAGACACATGAGTATCAGTGGGGAGTAATTTCTCAGCATCCTTATATTCTTGCTTCTTATTTATGGAATATGTTTGACTTTGCAGTTCCTCAATGGAAGCGTGGAGGTGTAGATGCGCGGAACATGAAAGGGTTGATAACTTTTGATCGTAAAATACGCAAGGATGCTTTCTATTGGTATAAGGCAAACTGGAGTAAGGAGCCGGTGCTTTATCTGACGCAACGCCGTAATACGGAACGTGAACGCCGGGAAACTTCTGTCACTGTCTATTCCAATCTTGGGACACCCCGCGTTTTCCTGAATGGCCGGGAACTGGATGGCGTTCGTAAGGGATATACCGATGTGCATTACATTTTTGATCAGGTAACGCTTGCCGAAGAACGAAATGTACTTAGAGCTGTTATCGTGTCTGATGGTAAGGAATATACCGATGAAATTGTGTGGAACTACAACGGTGAGCATGTCCGGCAGGCCGATGCTTACGAACATAAGAAAGAACATGCCGGCTGG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,456 total letters Query= CMR200052.106 Length=2448 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3680 0.0 1_03B_B6_CMR200052.106_T7term.seq 1743 0.0 2_06B_B6_CMR200052.106_T7S.seq 1725 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 57 ATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTG 146 2 11 ...........................................................---............................ 97 W S H P Q F E K G T E N L Y F Q S N A R E V T P F N N G W E Query_1 147 GTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCGTGAGGTAACTCCATTCAATAACGGATGGGAGTT 236 0 1 ................................... 35 0 897 ...........A.. 884 2 98 .......................................................................................... 187 F K K G P F P T D A M Q T A A R W N A D W E Q V N V P H T W Query_1 237 TAAGAAAGGGCCTTTTCCAACCGATGCGATGCAGACTGCTGCCAGATGGAATGCAGATTGGGAACAGGTGAATGTGCCTCATACCTGGAA 326 0 36 .......................................................................................... 125 0 1203 . 1203 2 188 .......................................................................................... 277 N A D D M Q K K T N S F Y A G V A Y Y R K H Y V F P E S L E Query_1 327 TGCGGATGATATGCAGAAGAAAACCAACAGCTTTTATGCTGGGGTAGCTTATTATCGGAAGCACTATGTTTTTCCTGAATCACTGGAAGG 416 0 126 .......................................................................................... 215 0 1202 ........... 1192 0 1826 ........... 1836 1 353 ........... 343 1 980 ........... 990 2 278 .......................................................................................... 367 G K R L F L R F E G V G A C A E V Y V N G Y L V G T H K G A Query_1 417 CAAGCGTCTTTTTCTACGCTTTGAGGGTGTAGGAGCATGTGCCGAAGTTTATGTCAACGGTTATTTGGTTGGAACACATAAAGGAGCTTA 506 0 216 .......................................................................................... 305 2 368 .......................................................................................... 457 Y S A F V C E I G S Q V R L G E E N E I V V K S D N V A R P Query_1 507 TTCTGCATTTGTCTGCGAAATTGGTTCGCAAGTCAGGCTGGGAGAAGAGAATGAAATTGTTGTCAAGTCCGATAATGTAGCACGTCCGGA 596 0 306 .......................................................................................... 395 2 458 .......................................................................................... 547 D V I P V N H A L F G V Y G G I Y R P V W L I V T E P C N I Query_1 597 TGTGATTCCGGTCAACCATGCTCTTTTTGGCGTCTATGGTGGAATTTACCGTCCCGTATGGCTGATAGTGACTGAACCCTGCAATATTGT 686 0 396 .......................................................................................... 485 2 548 .......................................................................................... 637 V V N D C A S P G V Y I T Q K N V S K Q S A D V S V R V K L Query_1 687 AGTGAATGACTGTGCCTCTCCGGGTGTATATATAACTCAGAAGAACGTATCGAAACAGTCTGCTGATG-TTTCTGTCAGGGTGAAACTTG 775 0 486 ....................................................................-..................... 574 2 638 .............................N.........N............................T..................... 727 D N A T L A P A A L E L E N A I Y T R E G K L V K V H R Q F Query_1 776 ATAATGCAACATTGGCTCCGGCTGCTCTTGAACTGGAAAATGCGATTTATACCCGTGAGGGCAAGTTGGTTAAAGTTCATCGGCAGTTTT 865 0 575 .......................................................................................... 664 0 1211 ........... 1221 1 971 ........... 961 2 728 .......................................................................................... 817 F E L T P Q G V Q N Y V S D F K I S H P H L W Q G R E D P Y Query_1 866 TTGAGCTGACTCCTCAGGGAGTACAAAACTATGTTTCTGATTTTAAAATCAGTCATCCTCATCTTTGGCAGGGGCGCGAAGAT-CCTTAT 954 0 665 ...................................................................................-...... 753 0 716 ................ 701 2 818 ........................................................-..........................C...... 906 2 869 ................ 854 L Y K V V S R L K Q D G Q V I D E V I Q P L G L R K Y E A Query_1 955 TTATATAAGGTCGTCTCTCGTCTGAAACAGGACGGACAGGTAA-TTGATGAAGTGATACAGCCACTCGGC-TTGCGTAAGTATGAGGCTG 1042 0 754 ...........................................-..........................-................... 841 2 907 ....N.-......N..................N..........N.......GT......N.-.N...C..T....NA....N.N...N.. 994 V A G K G F F L N G K K Y P M Y G V T R H Q D W W G Query_1 1043 TGGCTGGAAAAGGTTT-CTTCCTGAATGGTAAAAAATAT-CCGATGTAT--GGAGTTA-CCCGTCAT-CAGGACTGGTGGGGG-----AT 1121 0 842 ................-......................-.........--.......-........-...............-----.. 920 0 19 ..A..--.......-.. 6 0 1828 ........... 1838 1 351 ........... 341 2 995 ................C..C.........AT........NN.T......TG.C.....C........C..T....-C......CGTGT.. 1083 2 171 ..A..--.......-.. 158 M G S A L T N R E H D F D L E Q I M E V G A T T V R F A Query_1 1122 GGG-TAGCGC-ACTGACTAAC--AGGGAA-CATGATTTTGAT-CTGGAGCAGATTATGGAAGTTGGGGCAACGACCGTGCGTTTTGCTCA 1205 0 921 ...-......-..........--......-............-............................................... 1004 2 1084 .N.C...N..NN.....CT..NTN.C...N............N...N.N.CA...... 1141 H Y Q Q S D Y I Y S R C D T L G L I I W A E I P F V N R Query_1 1206 TTACCAGCAGTCGGATTATATTTATTCACGTTGTGATACGTTGGGACTTATTATCTGGGCTGAG---ATTCCTTTT--GTGAACCGTGT- 1289 0 1005 ................................................................---.........--...........- 1088 1 1145 ........NNGA.....NN..NN.......CN..N 1111 V T G Q E W D N V H Q Q M R E L I R Q S F N H P S Query_1 1290 -GACAGGGCAGG--AGTGGG-AT--AATGTT-CAT-CAGC--AAATGCGCG-AGCTG--ATACGT-CAGA-GTTTCAATCAT-CCTTCTA 1363 0 1089 -...........--......-..--......-...-....--.........-.....--......-....-...........-....... 1162 1 1110 G.C.N.......GA......G..TA......T...T....CA.........G..NNNGA......T....N...........T...NN.. 1021 I Y V W G I H N Q V Y H P H G Y T A A L T Q S V H D L C K L Query_1 1364 TTTATGTG-TGGGGAATTCATAACCAGGTGTATCATCCGCATGGTTATACGGCTGCTCTTACTCAGTCTGTTCACGATCTCTGCAAACTG 1452 0 1163 ........-...............G................................................................. 1251 0 136 ............ 125 0 593 ........... 603 1 1020 .......NN..................A.............N...ATN................................N......... 931 2 288 ............ 277 2 746 ........... 756 E D P D R Y T V A V N G Y G H A E H P V N G N T D I Q G Query_1 1453 GAAG-ATCCTGATCGTTATACGGTGGCTGTCAATG-GTTATGGCC-ATGCAGAACATCCGGTTAACGGGAATACCGATATACA-GGGAAT 1538 0 1252 ....-..............................-.........-.....................................-...... 1337 1 930 ....N...N.................N........N......N..C.....................................A...... 841 M N R Y F G W Y E K K I Q D I K P W I E K M A K E Y P W Q R Query_1 1539 GAACCGTTATTTCGGATGGTATGAGAAAAAGATACAGGATATCAAACCCTGGATTGAAAAAATGGCAAAAGAATATCCCTGGCAACGGCT 1628 0 1338 .......................................................................................... 1427 1 840 .............................................-............................................ 752 L M L T E Y G A D A N I E H Q T E L L G D A L N W G S D F Y Query_1 1629 GATGCTGACAGAATATGGGGCTGATGCGAACATTGAGCATCAGACAGAATTGCTGGGAGATGCCTTGAACTGGGGCAGTGATTTTTATCC 1718 0 1428 .......................................................................................... 1517 1 751 .......................................................................................... 662 P E T F Q T K T H E Y Q W G V I S Q H P Y I L A S Y L W N M Query_1 1719 GGAAACTTTCCAGACAAAGACACATGAGTATCAGTGGGGAGTAATTTCTCAGCATCCTTATATTCTTGCTTCTTATTTATGGAATATGTT 1808 0 1518 .......................................................................................... 1607 1 661 .......................................................................................... 572 F D F A V P Q W K R G G V D A R N M K G L I T F D R K I R K Query_1 1809 TGACTTTGCAGTTCCTCAATGGAAGCGTGGAGGTGTAGATGCGCGGAACATGAAAGGGTTGATAACTTTTGATCGTAAAATACGCAAGGA 1898 0 1608 .......................................................................................... 1697 1 571 .......................................................................................... 482 D A F Y W Y K A N W S K E P V L Y L T Q R R N T E R E R R E Query_1 1899 TGCTTTCTATTGGTATAAGGCAAACTGGAGTAAGGAGCCGGTGCTTTATCTGACGCAACGCCGTAATACGGAACGTGAACGCCGGGAAAC 1988 0 1698 .......................................................................................... 1787 1 481 .......................................................................................... 392 T S V T V Y S N L G T P R V F L N G R E L D G V R K G Y T D Query_1 1989 TTCTGTCACTGTCTATTCCAATCTTGGGACACCCCGCGTTTTCCTGAATGGCCGGGAACTGGATGGCGTTCGTAAGGGATATACCGATGT 2078 0 1788 .......................................................................................... 1877 0 1949 ......A........... 1966 0 195 ........... 205 0 859 ........... 869 1 391 .......................................................................................... 302 1 230 ......A........... 213 2 347 ........... 357 V H Y I F D Q V T L A E E R N V L R A V I V S D G K E Y T D Query_1 2079 GCATTACATTTTTGATCAGGTAACGCTTGCCGAAGAACGAAATGTACTTAGAGCTGTTATCGTGTCTGATGGTAAGGAATATACCGATGA 2168 0 1878 .......................................................................................... 1967 0 1859 ......G........... 1876 1 301 .......................................................................................... 212 1 320 ......G........... 303 E I V W N Y N G E H V R Q A D A Y E H K K E H A G W * H W K Query_1 2169 AATTGTGTGGAACTACAACGGTGAGCATGTCCGGCAGGCCGATGCTTACGAACATAAGAAAGAACATGCCGGCTGGTAACATTGGAAGTG 2258 0 1968 ............................................................................ 2043 1 211 ...................................................................N...................... 122 W I T D P N S S S V D K L A A A L E H H H H H H * D P A A N Query_1 2259 GATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGA-TCCGGCTGCTAACA 2347 1 121 ...........................................................................T.............. 32 K A R K E Query_1 2348 AAGCCCG-AAAGGAA 2361 1 31 .......A....... 17 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 10708555 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,456 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200062.106 (CMR200062.19-1423.P(1423).E780A.pMCSG68)
QREVPQSRMEQIYEEAKTPYKYGLAVAPADNKHKIDCPTVFREGDKWYMTYVVYNGKSGLDGRGYETWIAESDNLLEWRTLGRVLSYRDGFWDCNQRGGFPALPDMEWGGSYALQTYKGKHWMTYLGGEGTGYESVNKPLYIGLAWTDRPLGSAHEWQAQDQPVMSIHDKDAQWWEKLTQYKSVVYWDKEKTLGAPFVMFYNAAGRHPETDLKAERVGIALSKDMKKWKRYPGNPVFAHEADGTITGDAHIQKMGDVYVMFYFSAFEPSRKYKAFNTFAASYDLVHWTDWKGADLIIPSKDYDELFAHKSYVVKYNGVVYHFYCAVNDAEQRGIAIATSKPMGRSQVHFPEREVKNRRMVMELDKGWKTWLTEATHLKGLFAQKAIEVNIPHNWDDYYGYRQLTHGNLHGTAIYEKTFTLDDSQFLISNSSFGKRYFLRFEGVGTYATITLNGKDFGRHPVGRTTLTLDVTDALKLGENKLVVKAEHPEMIADMPWVCGGCSSEWGFSEGSQPLGIFRPVVLEATDEIRIEPFGVHIWNDDKAGTVFVETEVKNYGKTAETVEVVNKFSNADGKQVFRLTEKVTLQPGEKKVVKQQSPVQNPVLWSTENPYLYKLASMIKRGKSTTDEISTPFGIRTISWPVKREDGDGRFYLNGQPVFINGVCEYEHQFGQSHAFSREQVAARVKQIRAAGFNAFRDAHQPHHLDYQKYWDKEGVLWWTQFSAHVWYDTPEFRENFKKLLRQWVKERRNSPSVVMWGLQNESTLPKEFAEECSEIIREMDPTARTMRVITTCNGGDGTDWNVIQNWSGTYGGDVNKYGRELSQKNQLLNGEYGAWRSIGLHTEPAAFDVNGVWSEERMCQLMETKIRLAEQAKDSVCGQFQWIFSSHDNPGRRQPDEAYRRIDKVGPFNYKGLVTPWEEPLDVYYMYRANYVPASEDPMVYLASHTWEDRFATGRRRATIEAYSNCDSVLLYNDAVDAEYLGRKLNHGVGTHFMWENRDIRYNVLRAVGYFKGKPAAEDVLVLNGLEKAPHFEALYRGSAIVPVAADRLNGTDLLKGAEGYTYLYRLNCGGDAYTDTYGQVWAQDNSRYSHSWAESFVHPSDSVQLLSPYQASQRTTNDPIHGTRDWELFQTFRFGRHKLNFRFPVPDGEYRVELYFTEPWHGTGGGVQTDCEGLRIFDVAVNDKVLLDDLDVWAEAGHDGACKKVVNAIVKGGVLKINFPEVKAGQALICGIAIACKGDLDSVRSFSAHSFSWAAQDKEVMEKTPKELLPEDKNARANVTYQAEDAVLKGKFIKKEVKKQTGVFFGKGTQSSITWNISTGLAQVYALRFKYMNVTGKPMKVRMQFIDSKGVVLKEDNLTFAETPGKWRMLSTTTGTYINAGYYKVVLSAPDMEGLALDALDVQ
CAACGTGAAGTTCCGCAATCGCGCATGGAGCAGATTTATGAGGAAGCCAAGACTCCTTATAAATATGGGCTGGCTGTAGCTCCGGCCGACAACAAGCATAAAATAGACTGCCCTACCGTGTTCCGCGAAGGGGATAAGTGGTATATGACCTACGTGGTTTATAATGGTAAGAGCGGACTGGACGGACGTGGATACGAGACATGGATAGCCGAAAGTGATAATCTGTTGGAATGGCGTACATTGGGACGTGTCTTGTCTTATCGTGACGGATTCTGGGATTGCAACCAGCGTGGTGGTTTCCCTGCATTGCCGGATATGGAGTGGGGAGGAAGCTATGCCCTGCAGACGTATAAAGGCAAGCATTGGATGACTTATCTGGGTGGGGAAGGGACCGGATATGAATCGGTGAACAAGCCGCTGTATATTGGTTTGGCCTGGACAGACCGGCCTTTGGGTTCCGCCCATGAATGGCAGGCACAGGACCAGCCCGTGATGAGTATCCATGATAAAGACGCGCAATGGTGGGAAAAGCTGACTCAATATAAGAGTGTGGTCTATTGGGATAAGGAGAAAACATTGGGCGCACCTTTCGTCATGTTCTATAACGCGGCAGGACGGCATCCGGAAACGGATTTGAAAGCGGAACGGGTGGGTATCGCCTTGTCCAAAGATATGAAGAAATGGAAACGATATCCGGGCAATCCGGTCTTTGCACACGAAGCGGACGGAACCATTACGGGGGATGCGCATATCCAGAAAATGGGCGATGTGTATGTCATGTTCTATTTCTCGGCTTTTGAACCGAGCCGCAAATACAAGGCATTTAATACATTTGCTGCCAGTTATGACCTAGTACATTGGACGGATTGGAAAGGGGCGGATTTGATTATCCCTTCTAAAGACTATGATGAATTGTTCGCTCATAAGAGTTATGTGGTGAAATACAATGGGGTGGTGTATCATTTCTATTGTGCCGTGAATGACGCGGAACAACGTGGTATAGCCATTGCTACCAGCAAACCGATGGGACGTTCACAAGTGCATTTCCCGGAACGGGAGGTGAAGAATCGCCGGATGGTGATGGAATTGGATAAAGGATGGAAAACATGGCTGACGGAAGCAACTCATCTGAAAGGGTTGTTTGCTCAAAAGGCAATAGAAGTAAATATTCCTCATAACTGGGATGATTATTATGGCTACCGCCAACTGACACATGGTAATTTGCACGGTACTGCCATATACGAAAAGACCTTCACATTGGATGATTCTCAGTTTCTAATTTCCAACTCTTCATTCGGAAAACGTTATTTTCTTCGTTTTGAGGGAGTGGGGACGTATGCCACTATAACGTTGAATGGAAAGGATTTCGGCCGTCATCCCGTAGGGCGTACTACGCTGACATTGGATGTGACGGATGCGTTGAAGCTGGGAGAGAATAAGCTGGTAGTGAAAGCGGAACATCCTGAAATGATAGCCGACATGCCTTGGGTGTGCGGTGGCTGTTCCTCGGAATGGGGATTCAGCGAAGGTTCCCAGCCTTTGGGAATTTTCCGTCCCGTGGTGCTGGAGGCTACGGATGAGATCCGCATTGAGCCTTTCGGAGTGCATATCTGGAATGATGATAAGGCGGGAACTGTCTTTGTGGAAACGGAGGTGAAAAATTACGGAAAGACTGCTGAGACGGTGGAAGTGGTTAATAAATTCAGTAATGCGGATGGCAAGCAGGTGTTCCGCCTGACGGAGAAGGTGACTTTGCAGCCGGGTGAAAAGAAGGTGGTGAAGCAACAATCGCCAGTGCAGAATCCTGTTTTATGGAGTACGGAGAATCCTTATTTGTATAAACTGGCCAGTATGATAAAGCGTGGTAAGAGTACTACGGATGAGATTAGTACACCCTTTGGTATCCGTACCATCAGTTGGCCGGTGAAACGGGAGGATGGTGACGGACGGTTCTATCTGAACGGACAGCCTGTCTTTATCAATGGAGTGTGTGAATATGAACATCAGTTCGGACAAAGCCATGCTTTCAGCCGTGAGCAGGTGGCAGCCCGTGTGAAACAGATCCGTGCGGCGGGCTTCAACGCTTTCCGTGACGCTCACCAGCCCCATCACCTGGATTATCAGAAATATTGGGACAAGGAAGGAGTCTTGTGGTGGACTCAGTTTTCGGCCCACGTGTGGTATGATACTCCGGAGTTTCGTGAGAATTTCAAGAAACTGCTCCGCCAGTGGGTGAAAGAGCGTCGTAACTCGCCCAGTGTGGTGATGTGGGGATTGCAGAACGAAAGCACGCTGCCTAAGGAGTTTGCCGAAGAGTGTAGTGAGATTATCCGGGAAATGGATCCTACGGCCCGTACCATGCGTGTGATTACTACGTGTAACGGTGGTGATGGGACAGATTGGAATGTTATTCAGAACTGGAGTGGAACCTATGGTGGTGATGTGAATAAGTATGGCCGTGAATTGTCACAGAAGAATCAGTTGTTGAACGGTGAATATGGGGCATGGCGCAGCATCGGATTGCATACGGAACCGGCAGCTTTTGATGTGAATGGTGTATGGAGCGAGGAGCGTATGTGCCAGCTGATGGAAACCAAGATACGACTGGCGGAACAGGCAAAGGACAGTGTGTGCGGACAATTCCAATGGATATTCAGCAGTCATGATAATCCGGGACGCCGCCAGCCGGATGAAGCGTATCGCCGGATAGATAAGGTGGGACCATTTAATTATAAAGGATTGGTGACTCCGTGGGAGGAGCCGCTGGATGTATATTATATGTATCGCGCGAACTATGTGCCGGCTTCCGAAGATCCGATGGTTTATCTGGCATCTCATACATGGGAGGACCGTTTTGCGACAGGCCGCAGACGGGCTACCATTGAGGCGTACAGCAATTGTGACAGTGTGTTGCTGTACAATGATGCGGTGGATGCTGAATACTTGGGACGGAAATTGAATCATGGGGTGGGGACTCATTTCATGTGGGAAAACCGCGATATCCGTTACAATGTGCTTCGTGCTGTGGGGTACTTTAAAGGAAAACCGGCAGCGGAAGATGTGCTGGTGCTGAACGGTCTGGAAAAAGCTCCTCATTTTGAAGCTCTTTACCGTGGTTCTGCTATAGTGCCTGTTGCTGCCGACCGGTTGAACGGAACTGATTTGTTGAAAGGAGCTGAGGGATATACGTATCTTTATCGTTTGAATTGCGGAGGAGACGCTTATACGGATACGTATGGTCAGGTCTGGGCGCAGGATAACAGCCGTTATTCTCATTCGTGGGCGGAATCGTTTGTTCATCCGTCGGATAGTGTACAGTTGTTGAGCCCTTATCAGGCGAGCCAGCGTACCACGAATGATCCCATTCATGGCACTAGGGACTGGGAACTGTTCCAGACTTTCCGCTTCGGACGCCATAAGCTGAACTTCCGCTTTCCCGTTCCGGATGGCGAGTATCGTGTGGAACTTTATTTTACAGAACCCTGGCATGGTACAGGAGGCGGCGTACAGACGGATTGTGAAGGCTTGCGTATTTTTGATGTAGCGGTAAATGATAAAGTACTTCTGGACGACTTGGATGTATGGGCTGAAGCGGGTCATGACGGCGCTTGCAAAAAGGTGGTGAATGCTATCGTGAAAGGGGGAGTCTTGAAGATTAATTTTCCGGAGGTGAAAGCGGGGCAAGCTTTGATTTGTGGCATTGCGATAGCTTGTAAAGGCGACTTGGATTCCGTTCGCTCTTTTTCTGCCCATAGCTTCAGCTGGGCTGCACAAGACAAGGAGGTGATGGAAAAGACTCCCAAAGAATTATTGCCTGAAGATAAAAACGCACGTGCCAATGTGACTTATCAAGCGGAGGATGCTGTGCTGAAAGGTAAGTTCATAAAGAAAGAGGTTAAGAAACAAACCGGGGTTTTCTTTGGTAAAGGTACACAAAGCAGTATTACTTGGAACATTTCTACCGGTCTGGCACAAGTCTATGCTCTTCGTTTTAAATACATGAATGTTACAGGGAAGCCTATGAAGGTGCGTATGCAGTTCATTGATTCCAAAGGGGTGGTACTGAAGGAAGATAATCTGACTTTTGCCGAAACTCCCGGAAAGTGGCGTATGCTGAGTACAACGACGGGTACGTATATCAATGCAGGCTATTATAAGGTGGTGCTTTCCGCACCGGATATGGAGGGACTGGCATTGGATGCGCTGGATGTGCAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 6,689 total letters Query= CMR200062.106 Length=4620 Score E Sequences producing significant alignments: (Bits) Value 0_wt 7593 0.0 2_06C_C6_CMR200062.106_T7S.seq 1905 0.0 1_03C_C6_CMR200062.106_T7term.seq 1896 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 57 ATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTG 146 2 7 ...T..........-........................................................................... 95 W S H P Q F E K G T E N L Y F Q S N A Q R E V P Q S R M E Q Query_1 147 GTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAACGTGAAGTTCCGCAATCGCGCATGGAGCAGAT 236 0 1 ................................... 35 2 96 .......................................................................................... 185 I Y E E A K T P Y K Y G L A V A P A D N K H K I D C P T V F Query_1 237 TTATGAGGAAGCCAAGACTCCTTATAAATATGGGCTGGCTGTAGCTCCGGCCGACAACAAGCATAAAATAGACTGCCCTACCGTGTTCCG 326 0 36 .......................................................................................... 125 2 186 .......................................................................................... 275 R E G D K W Y M T Y V V Y N G K S G L D G R G Y E T W I A E Query_1 327 CGAAGGGGATAAGTGGTATATGACCTACGTGGTTTATAATGGTAAGAGCGGACTGGACGGACGTGGATACGAGACATGGATAGCCGAAAG 416 0 126 .......................................................................................... 215 2 276 .......................................................................................... 365 S D N L L E W R T L G R V L S Y R D G F W D C N Q R G G F P Query_1 417 TGATAATCTGTTGGAATGGCGTACATTGGGACGTGTCTTGTCTTATCGTGACGGATTCTGGGATTGCAACCAGCGTGGTGGTTTCCCTGC 506 0 216 .......................................................................................... 305 0 4159 ......C.....--.. 4172 2 366 .......................................................................................... 455 1 185 ......C.....--.. 172 A L P D M E W G G S Y A L Q T Y K G K H W M T Y L G G E G T Query_1 507 ATTGCCGGATATGGAGTGGGGAGGAAGCTATGCCCTGCAGACGTATAAAGGCAAGCATTGGATGACTTATCTGGGTGGGGAAGGGACCGG 596 0 306 .......................................................................................... 395 0 4173 .---............ 4185 2 456 .......................................................................................... 545 1 171 .---............ 159 G Y E S V N K P L Y I G L A W T D R P L G S A H E W Q A Q D Query_1 597 ATATGAATCGGTGAACAAGCCGCTGTATATTGGTTTGGCCTGGACAGACCGGCCTTTGGGTTCCGCCCATGAATGGCAGGCACAGGACCA 686 0 396 .......................................................................................... 485 2 546 .......................................................................................... 635 Q P V M S I H D K D A Q W W E K L T Q Y K S V V Y W D K E K Query_1 687 GCCCGTGATGAGTATCCATGATAAAGACGCGCAATGGTGGGAAAAGCTGACTCAATATAAGAGTGTGGTCTATTGGGATAAGGAGAAAAC 776 0 486 .......................................................................................... 575 2 636 .......................................................................................... 725 T L G A P F V M F Y N A A G R H P E T D L K A E R V G I A L Query_1 777 ATTGGGCGCACCTTTCGTCATGTTCTATAACGCGGCAGGACGGCATCCGGAAACGGATTTGAAAGCGGAACGGGTGGGTATCGCCTTGTC 866 0 576 .......................................................................................... 665 0 1448 ............ 1459 2 726 .......................................................................................... 815 2 924 ............TT.T.... 943 S K D M K K W K R Y P G N P V F A H E A D G T I T G D A H I Query_1 867 CAAAGATATGAAGAAATGGAAACGATATCCGGGCAATCCGGTCTTTGCACACGAAGCGGACGGAACCATTACGGGGGATGCGCATATCCA 956 0 666 .......................................................................................... 755 2 816 ........................................................................-................. 904 Q K M G D V Y V M F Y F S A F E P S R K Y K A F N T F A A S Query_1 957 GAAAATGGGCGATGTGTATGTCATGTTCTATTTCTCGGCTTTTGAACCGAGCCGCAAATACAAGGCATTTAATACATTTGCTGCCAGTTA 1046 0 756 .......................................................................................... 845 0 592 ............AA.G.... 611 2 905 ......................................................-N...............................-.. 992 2 742 ............AA.G.... 761 Y D L V H W T D W K G A D L I I P S K D Y D E L F A H K S Y Query_1 1047 TGACCTAGTACATTGGACGGATTGGAAAGGGGCGGATTTGATTATCCCTTCTAAAGACTATGATGAATTGTTCGCTCATAAGAGTTATGT 1136 0 846 .......................................................................................... 935 2 993 ............-............-..N.......-........-......-............-.............-.......... 1075 V V K Y N G V V Y H F Y C A V N D A E Q R G I A I A T S K P Query_1 1137 GGTGAAATACAATGGGGTGGTGTATCATTTCTATTGTGCCGTGAATGACGCGGAACAACGTGGTATAGCCATTGCTACCAGCAAACCGAT 1226 0 936 .......................................................................................... 1025 2 1076 ...-NN............-.N..NN......-....N.NNNNA........N.T.AC....NNN.-................-....N.. 1160 M G R S Q V H F P E R E V K N R R M V M E L D K G W K T W L Query_1 1227 GGGACGTTCACAAGTGCATTTCCCGGAACGGGAGGTGAAGAATCGCCGGATGGTGATGGAATTGGATAAAGGATGGAAAACATGGCTGAC 1316 0 1026 .......................................................................................... 1115 2 1161 ..A.... 1167 T E A T H L K G L F A Q K A I E V N I P H N W D D Y Y G Y R Query_1 1317 GGAAGCAACTCATCTGAAAGGGTTGTTTGCTCAAAAGGCAATAGAAGTAAATATTCCTCATAACTGGGATGATTATTATGGCTACCGCCA 1406 0 1116 .......................................................................................... 1205 Q L T H G N L H G T A I Y E K T F T L D D S Q F L I S N S S Query_1 1407 ACTGACACATGGTAATTTGCACGGTACTGCCATATACGAAAAGACCTTCACATTGGATGATTCTCAGTTTCTAATTTCCAACTCTTCATT 1496 0 1206 .......................................................................................... 1295 F G K R Y F L R F E G V G T Y A T I T L N G K D F G R H P V Query_1 1497 CGGAAAACGTTATTTTCTTCGTTTTGAGGGAGTGGGGACGTATGCCACTATAACGTTGAATGGAAAGGATTTCGGCCGTCATCCCGTAGG 1586 0 1296 .......................................................................................... 1385 G R T T L T L D V T D A L K L G E N K L V V K A E H P E M I Query_1 1587 GCGTACTACGCTGACATTGGATGTGACGGATGCGTTGAAGCTGGGAGAGAATAAGCTGGTAGTGAAAGCGGAACATCCTGAAATGATAGC 1676 0 1386 .......................................................................................... 1475 2 785 ............ 796 A D M P W V C G G C S S E W G F S E G S Q P L G I F R P V V Query_1 1677 CGACATGCCTTGGGTGTGCGGTGGCTGTTCCTCGGAATGGGGATTCAGCGAAGGTTCCCAGCCTTTGGGAATTTTCCGTCCCGTGGTGCT 1766 0 1476 .......................................................................................... 1565 L E A T D E I R I E P F G V H I W N D D K A G T V F V E T E Query_1 1767 GGAGGCTACGGATGAGATCCGCATTGAGCCTTTCGGAGTGCATATCTGGAATGATGATAAGGCGGGAACTGTCTTTGTGGAAACGGAGGT 1856 0 1566 .......................................................................................... 1655 0 1874 ............. 1886 V K N Y G K T A E T V E V V N K F S N A D G K Q V F R L T E Query_1 1857 GAAAAATTACGGAAAGACTGCTGAGACGGTGGAAGTGGTTAATAAATTCAGTAATGCGGATGGCAAGCAGGTGTTCCGCCTGACGGAGAA 1946 0 1656 .......................................................................................... 1745 K V T L Q P G E K K V V K Q Q S P V Q N P V L W S T E N P Y Query_1 1947 GGTGACTTTGCAGCCGGGTGAAAAGAAGGTGGTGAAGCAACAATCGCCAGTGCAGAATCCTGTTTTATGGAGTACGGAGAATCCTTATTT 2036 0 1746 .......................................................................................... 1835 L Y K L A S M I K R G K S T T D E I S T P F G I R T I S W P Query_1 2037 GTATAAACTGGCCAGTATGATAAAGCGTGGTAAGAGTACTACGGATGAGATTAGTACACCCTTTGGTATCCGTACCATCAGTTGGCCGGT 2126 0 1836 .......................................................................................... 1925 0 1571 ............. 1583 V K R E D G D G R F Y L N G Q P V F I N G V C E Y E H Q F G Query_1 2127 GAAACGGGAGGATGGTGACGGACGGTTCTATCTGAACGGACAGCCTGTCTTTATCAATGGAGTGTGTGAATATGAACATCAGTTCGGACA 2216 0 1926 .......................................................................................... 2015 Q S H A F S R E Q V A A R V K Q I R A A G F N A F R D A H Q Query_1 2217 AAGCCATGCTTTCAGCCGTGAGCAGGTGGCAGCCCGTGTGAAACAGATCCGTGCGGCGGGCTTCAACGCTTTCCGTGACGCTCACCAGCC 2306 0 2016 .......................................................................................... 2105 P H H L D Y Q K Y W D K E G V L W W T Q F S A H V W Y D T P Query_1 2307 CCATCACCTGGATTATCAGAAATATTGGGACAAGGAAGGAGTCTTGTGGTGGACTCAGTTTTCGGCCCACGTGTGGTATGATACTCCGGA 2396 0 2106 .......................................................................................... 2195 E F R E N F K K L L R Q W V K E R R N S P S V V M W G L Q N Query_1 2397 GTTTCGTGAGAATTTCAAGAAACTGCTCCGCCAGTGGGTGAAAGAGCGTCGTAACTCGCCCAGTGTGGTGATGTGGGGATTGCAGAACGC 2486 0 2196 .........................................................................................A 2285 A S T L P K E F A E E C S E I I R E M D P T A R T M R V I T Query_1 2487 TAGCACGCTGCCTAAGGAGTTTGCCGAAGAGTGTAGTGAGATTATCCGGGAAATGGATCCTACGGCCCGTACCATGCGTGTGATTACTAC 2576 0 2286 A......................................................................................... 2375 T C N G G D G T D W N V I Q N W S G T Y G G D V N K Y G R E Query_1 2577 GTGTAACGGTGGTGATGGGACAGATTGGAATGTTATTCAGAACTGGAGTGGAACCTATGGTGGTGATGTGAATAAGTATGGCCGTGAATT 2666 0 2376 .......................................................................................... 2465 L S Q K N Q L L N G E Y G A W R S I G L H T E P A A F D V N Query_1 2667 GTCACAGAAGAATCAGTTGTTGAACGGTGAATATGGGGCATGGCGCAGCATCGGATTGCATACGGAACCGGCAGCTTTTGATGTGAATGG 2756 0 2466 .......................................................................................... 2555 G V W S E E R M C Q L M E T K I R L A E Q A K D S V C G Q F Query_1 2757 TGTATGGAGCGAGGAGCGTATGTGCCAGCTGATGGAAACCAAGATACGACTGGCGGAACAGGCAAAGGACAGTGTGTGCGGACAATTCCA 2846 0 2556 .......................................................................................... 2645 Q W I F S S H D N P G R R Q P D E A Y R R I D K V G P F N Y Query_1 2847 ATGGATATTCAGCAGTCATGATAATCCGGGACGCCGCCAGCCGGATGAAGCGTATCGCCGGATAGATAAGGTGGGACCATTTAATTATAA 2936 0 2646 .......................................................................................... 2735 K G L V T P W E E P L D V Y Y M Y R A N Y V P A S E D P M V Query_1 2937 AGGATTGGTGACTCCGTGGGAGGAGCCGCTGGATGTATATTATATGTATCGCGCGAACTATGTGCCGGCTTCCGAAGATCCGATGGTTTA 3026 0 2736 .......................................................................................... 2825 Y L A S H T W E D R F A T G R R R A T I E A Y S N C D S V L Query_1 3027 TCTGGCATCTCATACATGGGAGGACCGTTTTGCGACAGGCCGCAGACGGGCTACCATTGAGGCGTACAGCAATTGTGACAGTGTGTTGCT 3116 0 2826 .......................................................................................... 2915 L Y N D A V D A E Y L G R K L N H G V G T H F M W E N R D I Query_1 3117 GTACAATGATGCGGTGGATGCTGAATACTTGGGACGGAAATTGAATCATGGGGTGGGGACTCATTTCATGTGGGAAAACCGCGATATCCG 3206 0 2916 .......................................................................................... 3005 R Y N V L R A V G Y F K G K P A A E D V L V L N G L E K A P Query_1 3207 TTACAATGTGCTTCGTGCTGTGGGGTACTTTAAAGGAAAACCGGCAGCGGAAGATGTGCTGGTGCTGAACGGTCTGGAAAAAGCTCCTCA 3296 0 3006 .......................................................................................... 3095 H F E A L Y R G S A I V P V A A D R L N G T D L L K G A E G Query_1 3297 TTTTGAAGCTCTTTACCGTGGTTCTGCTATAGTGCCTGTTGCTGCCGACCGGTTGAACGGAACTGATTTGTTGAAAGGAGCTGAGGGATA 3386 0 3096 .......................................................................................... 3185 1 1170 ......... 1162 Y T Y L Y R L N C G G D A Y T D T Y G Q V W A Q D N S R Y Query_1 3387 TA-CGTATCTTTATCGTTTGAATTGCGGAGGAG-ACGCTTAT-ACGGA-TACGTATGGTCAGGT-CTGGGCGCAGGATAACAGCCGTTAT 3471 0 3186 ..-..............................-........-.....-...............-......................... 3270 1 1161 ..A...N....-..NN..-..NN.....N....G..NGC...N.....N.....TAT....A..T....NN................... 1074 S H S W A E S F V H P S D S V Q L L S P Y Q A S Q R T T N Query_1 3472 TCTCATTCGTGGG-CGGAATCGTTTGTTCATCCGTCGGATAGTGTACAGTTGTTGAGCCCTTATCAGGCGAG-CCAGCGTACCACGAATG 3559 0 3271 .............-..........................................................-................. 3358 1 1073 ........NNN..N..........-.......-...............NN......AG..C...........G................. 986 D P I H G T R D W E L F Q T F R F G R H K L N F R F P V P D Query_1 3560 ATCCCATTCATGGCACTAGGGACTGGGAACTGTTCCAGACTTTCCGCTTCGGACGCCATAAGCTGAACTTCCGCTTTCCCGTTCCGGATG 3649 0 3359 .......................................................................................... 3448 1 985 ......N...............................GAC................................................. 896 G E Y R V E L Y F T E P W H G T G G G V Q T D C E G L R I F Query_1 3650 GCGAGTATCGTGTGGAACTTTATTTTACAGAACCCTGGCATGGTACAGGAGGCGGCGTACAGACGGATTGTGAAGGCTTGCGTATTTTTG 3739 0 3449 .......................................................................................... 3538 1 895 .......................................................................................... 806 D V A V N D K V L L D D L D V W A E A G H D G A C K K V V N Query_1 3740 ATGTAGCGGTAAATGATAAAGTACTTCTGGACGACTTGGATGTATGGGCTGAAGCGGGTCATGACGGCGCTTGCAAAAAGGTGGTGAATG 3829 0 3539 .......................................................................................... 3628 1 805 .......................................................................................... 716 A I V K G G V L K I N F P E V K A G Q A L I C G I A I A C K Query_1 3830 CTATCGTGAAAGGGGGAGTCTTGAAGATTAATTTTCCGGAGGTGAAAGCGGGGCAAGCTTTGATTTGTGGCATTGCGATAGCTTGTAAAG 3919 0 3629 .......................................................................................... 3718 1 715 .......................................................................................... 626 G D L D S V R S F S A H S F S W A A Q D K E V M E K T P K E Query_1 3920 GCGACTTGGATTCCGTTCGCTCTTTTTCTGCCCATAGCTTCAGCTGGGCTGCACAAGACAAGGAGGTGATGGAAAAGACTCCCAAAGAAT 4009 0 3719 .......................................................................................... 3808 1 625 .......................................................................................... 536 L L P E D K N A R A N V T Y Q A E D A V L K G K F I K K E V Query_1 4010 TATTGCCTGAAGATAAAAACGCACGTGCCAATGTGACTTATCAAGCGGAGGATGCTGTGCTGAAAGGTAAGTTCATAAAGAAAGAGGTTA 4099 0 3809 .......................................................................................... 3898 1 535 .......................................................................................... 446 K K Q T G V F F G K G T Q S S I T W N I S T G L A Q V Y A L Query_1 4100 AGAAACAAACCGGGGTTTTCTTTGGTAAAGGTACACAAAGCAGTATTACTTGGAACATTTCTACCGGTCTGGCACAAGTCTATGCTCTTC 4189 0 3899 .......................................................................................... 3988 1 445 .......................................................................................... 356 R F K Y M N V T G K P M K V R M Q F I D S K G V V L K E D N Query_1 4190 GTTTTAAATACATGAATGTTACAGGGAAGCCTATGAAGGTGCGTATGCAGTTCATTGATTCCAAAGGGGTGGTACTGAAGGAAGATAATC 4279 0 3989 .......................................................................................... 4078 1 355 .......................................................................................... 266 L T F A E T P G K W R M L S T T T G T Y I N A G Y Y K V V L Query_1 4280 TGACTTTTGCCGAAACTCCCGGAAAGTGGCGTATGCTGAGTACAACGACGGGTACGTATATCAATGCAGGCTATTATAAGGTGGTGCTTT 4369 0 4079 .......................................................................................... 4168 0 290 ......G... 299 2 440 ......G... 449 1 265 .......................................................................................... 176 S A P D M E G L A L D A L D V Q * H W K W I T D P N S S Query_1 4370 CC--GCA---CCGGATATGGAGGGACTGGCATTGGATGCGCTGGATGTGCAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTC 4454 0 4169 ..--...---.......................................... 4215 0 300 ..CT...TTG............ 321 2 450 ..CT...TTG............ 471 1 175 ..--...---................................................................................ 91 S V D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 4455 CGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGC 4535 1 90 ...........................................................................-..... 11 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 30534270 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 6,689 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200062.107 (CMR200062.19-1423.P(1423).E850A.pMCSG68)
QREVPQSRMEQIYEEAKTPYKYGLAVAPADNKHKIDCPTVFREGDKWYMTYVVYNGKSGLDGRGYETWIAESDNLLEWRTLGRVLSYRDGFWDCNQRGGFPALPDMEWGGSYALQTYKGKHWMTYLGGEGTGYESVNKPLYIGLAWTDRPLGSAHEWQAQDQPVMSIHDKDAQWWEKLTQYKSVVYWDKEKTLGAPFVMFYNAAGRHPETDLKAERVGIALSKDMKKWKRYPGNPVFAHEADGTITGDAHIQKMGDVYVMFYFSAFEPSRKYKAFNTFAASYDLVHWTDWKGADLIIPSKDYDELFAHKSYVVKYNGVVYHFYCAVNDAEQRGIAIATSKPMGRSQVHFPEREVKNRRMVMELDKGWKTWLTEATHLKGLFAQKAIEVNIPHNWDDYYGYRQLTHGNLHGTAIYEKTFTLDDSQFLISNSSFGKRYFLRFEGVGTYATITLNGKDFGRHPVGRTTLTLDVTDALKLGENKLVVKAEHPEMIADMPWVCGGCSSEWGFSEGSQPLGIFRPVVLEATDEIRIEPFGVHIWNDDKAGTVFVETEVKNYGKTAETVEVVNKFSNADGKQVFRLTEKVTLQPGEKKVVKQQSPVQNPVLWSTENPYLYKLASMIKRGKSTTDEISTPFGIRTISWPVKREDGDGRFYLNGQPVFINGVCEYEHQFGQSHAFSREQVAARVKQIRAAGFNAFRDAHQPHHLDYQKYWDKEGVLWWTQFSAHVWYDTPEFRENFKKLLRQWVKERRNSPSVVMWGLQNESTLPKEFAEECSEIIREMDPTARTMRVITTCNGGDGTDWNVIQNWSGTYGGDVNKYGRELSQKNQLLNGEYGAWRSIGLHTEPAAFDVNGVWSEERMCQLMETKIRLAEQAKDSVCGQFQWIFSSHDNPGRRQPDEAYRRIDKVGPFNYKGLVTPWEEPLDVYYMYRANYVPASEDPMVYLASHTWEDRFATGRRRATIEAYSNCDSVLLYNDAVDAEYLGRKLNHGVGTHFMWENRDIRYNVLRAVGYFKGKPAAEDVLVLNGLEKAPHFEALYRGSAIVPVAADRLNGTDLLKGAEGYTYLYRLNCGGDAYTDTYGQVWAQDNSRYSHSWAESFVHPSDSVQLLSPYQASQRTTNDPIHGTRDWELFQTFRFGRHKLNFRFPVPDGEYRVELYFTEPWHGTGGGVQTDCEGLRIFDVAVNDKVLLDDLDVWAEAGHDGACKKVVNAIVKGGVLKINFPEVKAGQALICGIAIACKGDLDSVRSFSAHSFSWAAQDKEVMEKTPKELLPEDKNARANVTYQAEDAVLKGKFIKKEVKKQTGVFFGKGTQSSITWNISTGLAQVYALRFKYMNVTGKPMKVRMQFIDSKGVVLKEDNLTFAETPGKWRMLSTTTGTYINAGYYKVVLSAPDMEGLALDALDVQ
CAACGTGAAGTTCCGCAATCGCGCATGGAGCAGATTTATGAGGAAGCCAAGACTCCTTATAAATATGGGCTGGCTGTAGCTCCGGCCGACAACAAGCATAAAATAGACTGCCCTACCGTGTTCCGCGAAGGGGATAAGTGGTATATGACCTACGTGGTTTATAATGGTAAGAGCGGACTGGACGGACGTGGATACGAGACATGGATAGCCGAAAGTGATAATCTGTTGGAATGGCGTACATTGGGACGTGTCTTGTCTTATCGTGACGGATTCTGGGATTGCAACCAGCGTGGTGGTTTCCCTGCATTGCCGGATATGGAGTGGGGAGGAAGCTATGCCCTGCAGACGTATAAAGGCAAGCATTGGATGACTTATCTGGGTGGGGAAGGGACCGGATATGAATCGGTGAACAAGCCGCTGTATATTGGTTTGGCCTGGACAGACCGGCCTTTGGGTTCCGCCCATGAATGGCAGGCACAGGACCAGCCCGTGATGAGTATCCATGATAAAGACGCGCAATGGTGGGAAAAGCTGACTCAATATAAGAGTGTGGTCTATTGGGATAAGGAGAAAACATTGGGCGCACCTTTCGTCATGTTCTATAACGCGGCAGGACGGCATCCGGAAACGGATTTGAAAGCGGAACGGGTGGGTATCGCCTTGTCCAAAGATATGAAGAAATGGAAACGATATCCGGGCAATCCGGTCTTTGCACACGAAGCGGACGGAACCATTACGGGGGATGCGCATATCCAGAAAATGGGCGATGTGTATGTCATGTTCTATTTCTCGGCTTTTGAACCGAGCCGCAAATACAAGGCATTTAATACATTTGCTGCCAGTTATGACCTAGTACATTGGACGGATTGGAAAGGGGCGGATTTGATTATCCCTTCTAAAGACTATGATGAATTGTTCGCTCATAAGAGTTATGTGGTGAAATACAATGGGGTGGTGTATCATTTCTATTGTGCCGTGAATGACGCGGAACAACGTGGTATAGCCATTGCTACCAGCAAACCGATGGGACGTTCACAAGTGCATTTCCCGGAACGGGAGGTGAAGAATCGCCGGATGGTGATGGAATTGGATAAAGGATGGAAAACATGGCTGACGGAAGCAACTCATCTGAAAGGGTTGTTTGCTCAAAAGGCAATAGAAGTAAATATTCCTCATAACTGGGATGATTATTATGGCTACCGCCAACTGACACATGGTAATTTGCACGGTACTGCCATATACGAAAAGACCTTCACATTGGATGATTCTCAGTTTCTAATTTCCAACTCTTCATTCGGAAAACGTTATTTTCTTCGTTTTGAGGGAGTGGGGACGTATGCCACTATAACGTTGAATGGAAAGGATTTCGGCCGTCATCCCGTAGGGCGTACTACGCTGACATTGGATGTGACGGATGCGTTGAAGCTGGGAGAGAATAAGCTGGTAGTGAAAGCGGAACATCCTGAAATGATAGCCGACATGCCTTGGGTGTGCGGTGGCTGTTCCTCGGAATGGGGATTCAGCGAAGGTTCCCAGCCTTTGGGAATTTTCCGTCCCGTGGTGCTGGAGGCTACGGATGAGATCCGCATTGAGCCTTTCGGAGTGCATATCTGGAATGATGATAAGGCGGGAACTGTCTTTGTGGAAACGGAGGTGAAAAATTACGGAAAGACTGCTGAGACGGTGGAAGTGGTTAATAAATTCAGTAATGCGGATGGCAAGCAGGTGTTCCGCCTGACGGAGAAGGTGACTTTGCAGCCGGGTGAAAAGAAGGTGGTGAAGCAACAATCGCCAGTGCAGAATCCTGTTTTATGGAGTACGGAGAATCCTTATTTGTATAAACTGGCCAGTATGATAAAGCGTGGTAAGAGTACTACGGATGAGATTAGTACACCCTTTGGTATCCGTACCATCAGTTGGCCGGTGAAACGGGAGGATGGTGACGGACGGTTCTATCTGAACGGACAGCCTGTCTTTATCAATGGAGTGTGTGAATATGAACATCAGTTCGGACAAAGCCATGCTTTCAGCCGTGAGCAGGTGGCAGCCCGTGTGAAACAGATCCGTGCGGCGGGCTTCAACGCTTTCCGTGACGCTCACCAGCCCCATCACCTGGATTATCAGAAATATTGGGACAAGGAAGGAGTCTTGTGGTGGACTCAGTTTTCGGCCCACGTGTGGTATGATACTCCGGAGTTTCGTGAGAATTTCAAGAAACTGCTCCGCCAGTGGGTGAAAGAGCGTCGTAACTCGCCCAGTGTGGTGATGTGGGGATTGCAGAACGAAAGCACGCTGCCTAAGGAGTTTGCCGAAGAGTGTAGTGAGATTATCCGGGAAATGGATCCTACGGCCCGTACCATGCGTGTGATTACTACGTGTAACGGTGGTGATGGGACAGATTGGAATGTTATTCAGAACTGGAGTGGAACCTATGGTGGTGATGTGAATAAGTATGGCCGTGAATTGTCACAGAAGAATCAGTTGTTGAACGGTGAATATGGGGCATGGCGCAGCATCGGATTGCATACGGAACCGGCAGCTTTTGATGTGAATGGTGTATGGAGCGAGGAGCGTATGTGCCAGCTGATGGAAACCAAGATACGACTGGCGGAACAGGCAAAGGACAGTGTGTGCGGACAATTCCAATGGATATTCAGCAGTCATGATAATCCGGGACGCCGCCAGCCGGATGAAGCGTATCGCCGGATAGATAAGGTGGGACCATTTAATTATAAAGGATTGGTGACTCCGTGGGAGGAGCCGCTGGATGTATATTATATGTATCGCGCGAACTATGTGCCGGCTTCCGAAGATCCGATGGTTTATCTGGCATCTCATACATGGGAGGACCGTTTTGCGACAGGCCGCAGACGGGCTACCATTGAGGCGTACAGCAATTGTGACAGTGTGTTGCTGTACAATGATGCGGTGGATGCTGAATACTTGGGACGGAAATTGAATCATGGGGTGGGGACTCATTTCATGTGGGAAAACCGCGATATCCGTTACAATGTGCTTCGTGCTGTGGGGTACTTTAAAGGAAAACCGGCAGCGGAAGATGTGCTGGTGCTGAACGGTCTGGAAAAAGCTCCTCATTTTGAAGCTCTTTACCGTGGTTCTGCTATAGTGCCTGTTGCTGCCGACCGGTTGAACGGAACTGATTTGTTGAAAGGAGCTGAGGGATATACGTATCTTTATCGTTTGAATTGCGGAGGAGACGCTTATACGGATACGTATGGTCAGGTCTGGGCGCAGGATAACAGCCGTTATTCTCATTCGTGGGCGGAATCGTTTGTTCATCCGTCGGATAGTGTACAGTTGTTGAGCCCTTATCAGGCGAGCCAGCGTACCACGAATGATCCCATTCATGGCACTAGGGACTGGGAACTGTTCCAGACTTTCCGCTTCGGACGCCATAAGCTGAACTTCCGCTTTCCCGTTCCGGATGGCGAGTATCGTGTGGAACTTTATTTTACAGAACCCTGGCATGGTACAGGAGGCGGCGTACAGACGGATTGTGAAGGCTTGCGTATTTTTGATGTAGCGGTAAATGATAAAGTACTTCTGGACGACTTGGATGTATGGGCTGAAGCGGGTCATGACGGCGCTTGCAAAAAGGTGGTGAATGCTATCGTGAAAGGGGGAGTCTTGAAGATTAATTTTCCGGAGGTGAAAGCGGGGCAAGCTTTGATTTGTGGCATTGCGATAGCTTGTAAAGGCGACTTGGATTCCGTTCGCTCTTTTTCTGCCCATAGCTTCAGCTGGGCTGCACAAGACAAGGAGGTGATGGAAAAGACTCCCAAAGAATTATTGCCTGAAGATAAAAACGCACGTGCCAATGTGACTTATCAAGCGGAGGATGCTGTGCTGAAAGGTAAGTTCATAAAGAAAGAGGTTAAGAAACAAACCGGGGTTTTCTTTGGTAAAGGTACACAAAGCAGTATTACTTGGAACATTTCTACCGGTCTGGCACAAGTCTATGCTCTTCGTTTTAAATACATGAATGTTACAGGGAAGCCTATGAAGGTGCGTATGCAGTTCATTGATTCCAAAGGGGTGGTACTGAAGGAAGATAATCTGACTTTTGCCGAAACTCCCGGAAAGTGGCGTATGCTGAGTACAACGACGGGTACGTATATCAATGCAGGCTATTATAAGGTGGTGCTTTCCGCACCGGATATGGAGGGACTGGCATTGGATGCGCTGGATGTGCAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 6,700 total letters Query= CMR200062.107 Length=4620 Score E Sequences producing significant alignments: (Bits) Value 0_wt 7593 0.0 1_03D_D6_CMR200062.107_T7term.seq 1914 0.0 2_06D_D6_CMR200062.107_T7S.seq 1678 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 57 ATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTG 146 2 6 ..............-........................................................................... 94 W S H P Q F E K G T E N L Y F Q S N A Q R E V P Q S R M E Q Query_1 147 GTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAACGTGAAGTTCCGCAATCGCGCATGGAGCAGAT 236 0 1 ................................... 35 2 95 .......................................................................................... 184 I Y E E A K T P Y K Y G L A V A P A D N K H K I D C P T V F Query_1 237 TTATGAGGAAGCCAAGACTCCTTATAAATATGGGCTGGCTGTAGCTCCGGCCGACAACAAGCATAAAATAGACTGCCCTACCGTGTTCCG 326 0 36 .......................................................................................... 125 2 185 .......................................................................................... 274 R E G D K W Y M T Y V V Y N G K S G L D G R G Y E T W I A E Query_1 327 CGAAGGGGATAAGTGGTATATGACCTACGTGGTTTATAATGGTAAGAGCGGACTGGACGGACGTGGATACGAGACATGGATAGCCGAAAG 416 0 126 .......................................................................................... 215 2 275 .......................................................................................... 364 S D N L L E W R T L G R V L S Y R D G F W D C N Q R G G F P Query_1 417 TGATAATCTGTTGGAATGGCGTACATTGGGACGTGTCTTGTCTTATCGTGACGGATTCTGGGATTGCAACCAGCGTGGTGGTTTCCCTGC 506 0 216 .......................................................................................... 305 0 4159 ......C.....--.. 4172 1 188 ......C.....--.. 175 2 365 .......................................................................................... 454 A L P D M E W G G S Y A L Q T Y K G K H W M T Y L G G E G T Query_1 507 ATTGCCGGATATGGAGTGGGGAGGAAGCTATGCCCTGCAGACGTATAAAGGCAAGCATTGGATGACTTATCTGGGTGGGGAAGGGACCGG 596 0 306 .......................................................................................... 395 0 4173 .---............ 4185 1 174 .---............ 162 2 455 .......................................................................................... 544 G Y E S V N K P L Y I G L A W T D R P L G S A H E W Q A Q D Query_1 597 ATATGAATCGGTGAACAAGCCGCTGTATATTGGTTTGGCCTGGACAGACCGGCCTTTGGGTTCCGCCCATGAATGGCAGGCACAGGACCA 686 0 396 .......................................................................................... 485 2 545 .......................................................................................... 634 Q P V M S I H D K D A Q W W E K L T Q Y K S V V Y W D K E K Query_1 687 GCCCGTGATGAGTATCCATGATAAAGACGCGCAATGGTGGGAAAAGCTGACTCAATATAAGAGTGTGGTCTATTGGGATAAGGAGAAAAC 776 0 486 .......................................................................................... 575 2 635 .......................................................................................... 724 T L G A P F V M F Y N A A G R H P E T D L K A E R V G I A Query_1 777 ATTGGGCGCACC-TTTCGTCATGTTCTATAACGCGGCAGGACGGCATCCGGAAACGGA-TTTGAAAGCGGAACGGG-TGGGTATCGCCTT 863 0 576 ............-.............................................-.................-............. 662 0 1448 ............ 1459 2 725 ............T.............................................T.................N............. 814 L S K D M K K W K R Y P G N P V F A H E A D G T I T G D A Query_1 864 GTCCAAAGATATGAAGAAA-TGGAAACGATATCCGGGCAAT-CCGGTC-TTTGCACACGAAGCGGACGGAACCATTACGGGGGATGCGCA 950 0 663 ...................-.....................-......-......................................... 749 2 815 ..N.C..............T.....................C......T.....N.......................-.....N..... 903 H I Q K M G D V Y V M F Y F S A F E P S R K Y K A F N T F A Query_1 951 TATCCAGAAAATGGGCGATGTGTATGTCATGTTC-TATTTCTCGGCTTTTGAACCGAGCCGCAAATACAAGGCATTTAATACATTTGCTG 1039 0 750 ..................................-....................................................... 838 0 592 .........-...AA.G.... 611 2 904 ...T..................-N..........T........-.......-..........-....TN.-...........N....... 988 2 742 .........-...AA.G.... 761 A S Y D L V H W T D W K G A D L I I P S K D Y D E L F A H K Query_1 1040 CCAGTTATGACCTAGTACATTGGACGGATTGGAAAGGGGCGGATTTGATTATCCCTTCTAAAGACTATGATGAATTGTTCGCTCATAAGA 1129 0 839 .......................................................................................... 928 2 989 ....-.........-.N.....NNN.......N..-..N..A...G...A...T..NTN..NA.AN......-.N..... 1064 S Y V V K Y N G V V Y H F Y C A V N D A E Q R G I A I A T S Query_1 1130 GTTATGTGGTGAAATACAATGGGGTGGTGTATCATTTCTATTGTGCCGTGAATGACGCGGAACAACGTGGTATAGCCATTGCTACCAGCA 1219 0 929 .......................................................................................... 1018 K P M G R S Q V H F P E R E V K N R R M V M E L D K G W K T Query_1 1220 AACCGATGGGACGTTCACAAGTGCATTTCCCGGAACGGGAGGTGAAGAATCGCCGGATGGTGATGGAATTGGATAAAGGATGGAAAACAT 1309 0 1019 .......................................................................................... 1108 1 902 ............. 914 W L T E A T H L K G L F A Q K A I E V N I P H N W D D Y Y G Query_1 1310 GGCTGACGGAAGCAACTCATCTGAAAGGGTTGTTTGCTCAAAAGGCAATAGAAGTAAATATTCCTCATAACTGGGATGATTATTATGGCT 1399 0 1109 .......................................................................................... 1198 Y R Q L T H G N L H G T A I Y E K T F T L D D S Q F L I S N Query_1 1400 ACCGCCAACTGACACATGGTAATTTGCACGGTACTGCCATATACGAAAAGACCTTCACATTGGATGATTCTCAGTTTCTAATTTCCAACT 1489 0 1199 .......................................................................................... 1288 S S F G K R Y F L R F E G V G T Y A T I T L N G K D F G R H Query_1 1490 CTTCATTCGGAAAACGTTATTTTCTTCGTTTTGAGGGAGTGGGGACGTATGCCACTATAACGTTGAATGGAAAGGATTTCGGCCGTCATC 1579 0 1289 .......................................................................................... 1378 P V G R T T L T L D V T D A L K L G E N K L V V K A E H P E Query_1 1580 CCGTAGGGCGTACTACGCTGACATTGGATGTGACGGATGCGTTGAAGCTGGGAGAGAATAAGCTGGTAGTGAAAGCGGAACATCCTGAAA 1669 0 1379 .......................................................................................... 1468 2 786 ............ 797 M I A D M P W V C G G C S S E W G F S E G S Q P L G I F R P Query_1 1670 TGATAGCCGACATGCCTTGGGTGTGCGGTGGCTGTTCCTCGGAATGGGGATTCAGCGAAGGTTCCCAGCCTTTGGGAATTTTCCGTCCCG 1759 0 1469 .......................................................................................... 1558 V V L E A T D E I R I E P F G V H I W N D D K A G T V F V E Query_1 1760 TGGTGCTGGAGGCTACGGATGAGATCCGCATTGAGCCTTTCGGAGTGCATATCTGGAATGATGATAAGGCGGGAACTGTCTTTGTGGAAA 1849 0 1559 .......................................................................................... 1648 0 1874 ............. 1886 T E V K N Y G K T A E T V E V V N K F S N A D G K Q V F R L Query_1 1850 CGGAGGTGAAAAATTACGGAAAGACTGCTGAGACGGTGGAAGTGGTTAATAAATTCAGTAATGCGGATGGCAAGCAGGTGTTCCGCCTGA 1939 0 1649 .......................................................................................... 1738 T E K V T L Q P G E K K V V K Q Q S P V Q N P V L W S T E N Query_1 1940 CGGAGAAGGTGACTTTGCAGCCGGGTGAAAAGAAGGTGGTGAAGCAACAATCGCCAGTGCAGAATCCTGTTTTATGGAGTACGGAGAATC 2029 0 1739 .......................................................................................... 1828 P Y L Y K L A S M I K R G K S T T D E I S T P F G I R T I S Query_1 2030 CTTATTTGTATAAACTGGCCAGTATGATAAAGCGTGGTAAGAGTACTACGGATGAGATTAGTACACCCTTTGGTATCCGTACCATCAGTT 2119 0 1829 .......................................................................................... 1918 0 1571 ............. 1583 W P V K R E D G D G R F Y L N G Q P V F I N G V C E Y E H Q Query_1 2120 GGCCGGTGAAACGGGAGGATGGTGACGGACGGTTCTATCTGAACGGACAGCCTGTCTTTATCAATGGAGTGTGTGAATATGAACATCAGT 2209 0 1919 .......................................................................................... 2008 F G Q S H A F S R E Q V A A R V K Q I R A A G F N A F R D A Query_1 2210 TCGGACAAAGCCATGCTTTCAGCCGTGAGCAGGTGGCAGCCCGTGTGAAACAGATCCGTGCGGCGGGCTTCAACGCTTTCCGTGACGCTC 2299 0 2009 .......................................................................................... 2098 H Q P H H L D Y Q K Y W D K E G V L W W T Q F S A H V W Y D Query_1 2300 ACCAGCCCCATCACCTGGATTATCAGAAATATTGGGACAAGGAAGGAGTCTTGTGGTGGACTCAGTTTTCGGCCCACGTGTGGTATGATA 2389 0 2099 .......................................................................................... 2188 T P E F R E N F K K L L R Q W V K E R R N S P S V V M W G L Query_1 2390 CTCCGGAGTTTCGTGAGAATTTCAAGAAACTGCTCCGCCAGTGGGTGAAAGAGCGTCGTAACTCGCCCAGTGTGGTGATGTGGGGATTGC 2479 0 2189 .......................................................................................... 2278 Q N E S T L P K E F A E E C S E I I R E M D P T A R T M R V Query_1 2480 AGAACGAAAGCACGCTGCCTAAGGAGTTTGCCGAAGAGTGTAGTGAGATTATCCGGGAAATGGATCCTACGGCCCGTACCATGCGTGTGA 2569 0 2279 .......................................................................................... 2368 I T T C N G G D G T D W N V I Q N W S G T Y G G D V N K Y G Query_1 2570 TTACTACGTGTAACGGTGGTGATGGGACAGATTGGAATGTTATTCAGAACTGGAGTGGAACCTATGGTGGTGATGTGAATAAGTATGGCC 2659 0 2369 .......................................................................................... 2458 R E L S Q K N Q L L N G A Y G A W R S I G L H T E P A A F D Query_1 2660 GTGAATTGTCACAGAAGAATCAGTTGTTGAACGGTGCTTATGGGGCATGGCGCAGCATCGGATTGCATACGGAACCGGCAGCTTTTGATG 2749 0 2459 ....................................AA.................................................... 2548 V N G V W S E E R M C Q L M E T K I R L A E Q A K D S V C G Query_1 2750 TGAATGGTGTATGGAGCGAGGAGCGTATGTGCCAGCTGATGGAAACCAAGATACGACTGGCGGAACAGGCAAAGGACAGTGTGTGCGGAC 2839 0 2549 .......................................................................................... 2638 Q F Q W I F S S H D N P G R R Q P D E A Y R R I D K V G P F Query_1 2840 AATTCCAATGGATATTCAGCAGTCATGATAATCCGGGACGCCGCCAGCCGGATGAAGCGTATCGCCGGATAGATAAGGTGGGACCATTTA 2929 0 2639 .......................................................................................... 2728 N Y K G L V T P W E E P L D V Y Y M Y R A N Y V P A S E D P Query_1 2930 ATTATAAAGGATTGGTGACTCCGTGGGAGGAGCCGCTGGATGTATATTATATGTATCGCGCGAACTATGTGCCGGCTTCCGAAGATCCGA 3019 0 2729 .......................................................................................... 2818 M V Y L A S H T W E D R F A T G R R R A T I E A Y S N C D S Query_1 3020 TGGTTTATCTGGCATCTCATACATGGGAGGACCGTTTTGCGACAGGCCGCAGACGGGCTACCATTGAGGCGTACAGCAATTGTGACAGTG 3109 0 2819 .......................................................................................... 2908 V L L Y N D A V D A E Y L G R K L N H G V G T H F M W E N R Query_1 3110 TGTTGCTGTACAATGATGCGGTGGATGCTGAATACTTGGGACGGAAATTGAATCATGGGGTGGGGACTCATTTCATGTGGGAAAACCGCG 3199 0 2909 .......................................................................................... 2998 D I R Y N V L R A V G Y F K G K P A A E D V L V L N G L E K Query_1 3200 ATATCCGTTACAATGTGCTTCGTGCTGTGGGGTACTTTAAAGGAAAACCGGCAGCGGAAGATGTGCTGGTGCTGAACGGTCTGGAAAAAG 3289 0 2999 .......................................................................................... 3088 A P H F E A L Y R G S A I V P V A A D R L N G T D L L K G A Query_1 3290 CTCCTCATTTTGAAGCTCTTTACCGTGGTTCTGCTATAGTGCCTGTTGCTGCCGACCGGTTGAACGGAACTGATTTGTTGAAAGGAGCTG 3379 0 3089 .......................................................................................... 3178 E G Y T Y L Y R L N C G G D A Y T D T Y G Q V W A Q D N Query_1 3380 AGGGATATACGTATCTTTATCGTTTGAATTGCGG-AGGAGAC-GCTT-ATACGGATA-CGTATGGTCAGGTCTGGGCGCAGGA-TAACAG 3464 0 3179 ..................................-.......-....-.........-.........................-...... 3263 1 1155 ......NNN..N..N.....NNN....N..N......A.........................A...... 1086 S R Y S H S W A E S F V H P S D S V Q L L S P Y Q A S Q R T Query_1 3465 CCGTTATTCTCATTCGTGGG-CGGAATCGTTTGTTCATCCGTCGGATAGT-GTACAGTTGTTGAGCCCTTATCAGGCGAGCCAGCGTACC 3552 0 3264 ....................-.............................-....................................... 3351 1 1085 .......NT..N.-......G.............-...............N..........G.N............N............. 998 T N D P I H G T R D W E L F Q T F R F G R H K L N F R F P V Query_1 3553 ACGAATGATCCCATTCATGGCACTAGGGACTGGGAACTGTTCCAGACTTTCCGCTTCGGACGCCATAAGCTGAACTTCCGCTTTCCCGTT 3642 0 3352 .......................................................................................... 3441 1 997 .......................................................................................... 908 P D G E Y R V E L Y F T E P W H G T G G G V Q T D C E G L Query_1 3643 CCGG-ATGGCGAGTATCGTGTGGAACTTTATTTTACAGAACCCTGGCATGGTACAGGAGGCGGCGTACAGACGGATTGTGAAGG-CTTGC 3730 0 3442 ....-...............................................................................-..... 3529 1 907 ....G...........................................................................G...G..... 818 R I F D V A V N D K V L L D D L D V W A E A G H D G A C K K Query_1 3731 GTATTTTTGATGTAGCGGTAAATGATAAAGTACTTCTGGACGACTTGGATGTATGGGCTGAAGCGGGTCATGACGGCGCTTGCAAAAAGG 3820 0 3530 .......................................................................................... 3619 1 817 .......................................................................................... 728 V V N A I V K G G V L K I N F P E V K A G Q A L I C G I A I Query_1 3821 TGGTGAATGCTATCGTGAAAGGGGGAGTCTTGAAGATTAATTTTCCGGAGGTGAAAGCGGGGCAAGCTTTGATTTGTGGCATTGCGATAG 3910 0 3620 .......................................................................................... 3709 1 727 .......................................................................................... 638 A C K G D L D S V R S F S A H S F S W A A Q D K E V M E K T Query_1 3911 CTTGTAAAGGCGACTTGGATTCCGTTCGCTCTTTTTCTGCCCATAGCTTCAGCTGGGCTGCACAAGACAAGGAGGTGATGGAAAAGACTC 4000 0 3710 .......................................................................................... 3799 1 637 .......................................................................................... 548 P K E L L P E D K N A R A N V T Y Q A E D A V L K G K F I K Query_1 4001 CCAAAGAATTATTGCCTGAAGATAAAAACGCACGTGCCAATGTGACTTATCAAGCGGAGGATGCTGTGCTGAAAGGTAAGTTCATAAAGA 4090 0 3800 .......................................................................................... 3889 1 547 .......................................................................................... 458 K E V K K Q T G V F F G K G T Q S S I T W N I S T G L A Q V Query_1 4091 AAGAGGTTAAGAAACAAACCGGGGTTTTCTTTGGTAAAGGTACACAAAGCAGTATTACTTGGAACATTTCTACCGGTCTGGCACAAGTCT 4180 0 3890 .......................................................................................... 3979 1 457 .......................................................................................... 368 Y A L R F K Y M N V T G K P M K V R M Q F I D S K G V V L K Query_1 4181 ATGCTCTTCGTTTTAAATACATGAATGTTACAGGGAAGCCTATGAAGGTGCGTATGCAGTTCATTGATTCCAAAGGGGTGGTACTGAAGG 4270 0 3980 .......................................................................................... 4069 1 367 .......................................................................................... 278 E D N L T F A E T P G K W R M L S T T T G T Y I N A G Y Y K Query_1 4271 AAGATAATCTGACTTTTGCCGAAACTCCCGGAAAGTGGCGTATGCTGAGTACAACGACGGGTACGTATATCAATGCAGGCTATTATAAGG 4360 0 4070 .......................................................................................... 4159 0 290 . 290 1 277 .......................................................................................... 188 2 439 . 439 V V L S A P D M E G L A L D A L D V Q * H W K W I T D P Query_1 4361 TGGTGCTTTCC--GCA---CCGGATATGGAGGGACTGGCATTGGATGCGCTGGATGTGCAATAACATTGGAAGTGGATAACGGATCCGAA 4445 0 4160 ...........--...---.......................................... 4215 0 291 .....G.....CT...TTG............ 321 1 187 ...........--...---....................................................................... 103 2 440 .....G.....CT...TTG............ 470 N S S S V D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 4446 TTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAG-CCCGAAAGGAAG 4534 1 102 .............................................................................C............ 13 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 30584892 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 6,700 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200062.108 (CMR200062.19-1423.P(1423).E780Q.pMCSG68)
QREVPQSRMEQIYEEAKTPYKYGLAVAPADNKHKIDCPTVFREGDKWYMTYVVYNGKSGLDGRGYETWIAESDNLLEWRTLGRVLSYRDGFWDCNQRGGFPALPDMEWGGSYALQTYKGKHWMTYLGGEGTGYESVNKPLYIGLAWTDRPLGSAHEWQAQDQPVMSIHDKDAQWWEKLTQYKSVVYWDKEKTLGAPFVMFYNAAGRHPETDLKAERVGIALSKDMKKWKRYPGNPVFAHEADGTITGDAHIQKMGDVYVMFYFSAFEPSRKYKAFNTFAASYDLVHWTDWKGADLIIPSKDYDELFAHKSYVVKYNGVVYHFYCAVNDAEQRGIAIATSKPMGRSQVHFPEREVKNRRMVMELDKGWKTWLTEATHLKGLFAQKAIEVNIPHNWDDYYGYRQLTHGNLHGTAIYEKTFTLDDSQFLISNSSFGKRYFLRFEGVGTYATITLNGKDFGRHPVGRTTLTLDVTDALKLGENKLVVKAEHPEMIADMPWVCGGCSSEWGFSEGSQPLGIFRPVVLEATDEIRIEPFGVHIWNDDKAGTVFVETEVKNYGKTAETVEVVNKFSNADGKQVFRLTEKVTLQPGEKKVVKQQSPVQNPVLWSTENPYLYKLASMIKRGKSTTDEISTPFGIRTISWPVKREDGDGRFYLNGQPVFINGVCEYEHQFGQSHAFSREQVAARVKQIRAAGFNAFRDAHQPHHLDYQKYWDKEGVLWWTQFSAHVWYDTPEFRENFKKLLRQWVKERRNSPSVVMWGLQNESTLPKEFAEECSEIIREMDPTARTMRVITTCNGGDGTDWNVIQNWSGTYGGDVNKYGRELSQKNQLLNGEYGAWRSIGLHTEPAAFDVNGVWSEERMCQLMETKIRLAEQAKDSVCGQFQWIFSSHDNPGRRQPDEAYRRIDKVGPFNYKGLVTPWEEPLDVYYMYRANYVPASEDPMVYLASHTWEDRFATGRRRATIEAYSNCDSVLLYNDAVDAEYLGRKLNHGVGTHFMWENRDIRYNVLRAVGYFKGKPAAEDVLVLNGLEKAPHFEALYRGSAIVPVAADRLNGTDLLKGAEGYTYLYRLNCGGDAYTDTYGQVWAQDNSRYSHSWAESFVHPSDSVQLLSPYQASQRTTNDPIHGTRDWELFQTFRFGRHKLNFRFPVPDGEYRVELYFTEPWHGTGGGVQTDCEGLRIFDVAVNDKVLLDDLDVWAEAGHDGACKKVVNAIVKGGVLKINFPEVKAGQALICGIAIACKGDLDSVRSFSAHSFSWAAQDKEVMEKTPKELLPEDKNARANVTYQAEDAVLKGKFIKKEVKKQTGVFFGKGTQSSITWNISTGLAQVYALRFKYMNVTGKPMKVRMQFIDSKGVVLKEDNLTFAETPGKWRMLSTTTGTYINAGYYKVVLSAPDMEGLALDALDVQ
CAACGTGAAGTTCCGCAATCGCGCATGGAGCAGATTTATGAGGAAGCCAAGACTCCTTATAAATATGGGCTGGCTGTAGCTCCGGCCGACAACAAGCATAAAATAGACTGCCCTACCGTGTTCCGCGAAGGGGATAAGTGGTATATGACCTACGTGGTTTATAATGGTAAGAGCGGACTGGACGGACGTGGATACGAGACATGGATAGCCGAAAGTGATAATCTGTTGGAATGGCGTACATTGGGACGTGTCTTGTCTTATCGTGACGGATTCTGGGATTGCAACCAGCGTGGTGGTTTCCCTGCATTGCCGGATATGGAGTGGGGAGGAAGCTATGCCCTGCAGACGTATAAAGGCAAGCATTGGATGACTTATCTGGGTGGGGAAGGGACCGGATATGAATCGGTGAACAAGCCGCTGTATATTGGTTTGGCCTGGACAGACCGGCCTTTGGGTTCCGCCCATGAATGGCAGGCACAGGACCAGCCCGTGATGAGTATCCATGATAAAGACGCGCAATGGTGGGAAAAGCTGACTCAATATAAGAGTGTGGTCTATTGGGATAAGGAGAAAACATTGGGCGCACCTTTCGTCATGTTCTATAACGCGGCAGGACGGCATCCGGAAACGGATTTGAAAGCGGAACGGGTGGGTATCGCCTTGTCCAAAGATATGAAGAAATGGAAACGATATCCGGGCAATCCGGTCTTTGCACACGAAGCGGACGGAACCATTACGGGGGATGCGCATATCCAGAAAATGGGCGATGTGTATGTCATGTTCTATTTCTCGGCTTTTGAACCGAGCCGCAAATACAAGGCATTTAATACATTTGCTGCCAGTTATGACCTAGTACATTGGACGGATTGGAAAGGGGCGGATTTGATTATCCCTTCTAAAGACTATGATGAATTGTTCGCTCATAAGAGTTATGTGGTGAAATACAATGGGGTGGTGTATCATTTCTATTGTGCCGTGAATGACGCGGAACAACGTGGTATAGCCATTGCTACCAGCAAACCGATGGGACGTTCACAAGTGCATTTCCCGGAACGGGAGGTGAAGAATCGCCGGATGGTGATGGAATTGGATAAAGGATGGAAAACATGGCTGACGGAAGCAACTCATCTGAAAGGGTTGTTTGCTCAAAAGGCAATAGAAGTAAATATTCCTCATAACTGGGATGATTATTATGGCTACCGCCAACTGACACATGGTAATTTGCACGGTACTGCCATATACGAAAAGACCTTCACATTGGATGATTCTCAGTTTCTAATTTCCAACTCTTCATTCGGAAAACGTTATTTTCTTCGTTTTGAGGGAGTGGGGACGTATGCCACTATAACGTTGAATGGAAAGGATTTCGGCCGTCATCCCGTAGGGCGTACTACGCTGACATTGGATGTGACGGATGCGTTGAAGCTGGGAGAGAATAAGCTGGTAGTGAAAGCGGAACATCCTGAAATGATAGCCGACATGCCTTGGGTGTGCGGTGGCTGTTCCTCGGAATGGGGATTCAGCGAAGGTTCCCAGCCTTTGGGAATTTTCCGTCCCGTGGTGCTGGAGGCTACGGATGAGATCCGCATTGAGCCTTTCGGAGTGCATATCTGGAATGATGATAAGGCGGGAACTGTCTTTGTGGAAACGGAGGTGAAAAATTACGGAAAGACTGCTGAGACGGTGGAAGTGGTTAATAAATTCAGTAATGCGGATGGCAAGCAGGTGTTCCGCCTGACGGAGAAGGTGACTTTGCAGCCGGGTGAAAAGAAGGTGGTGAAGCAACAATCGCCAGTGCAGAATCCTGTTTTATGGAGTACGGAGAATCCTTATTTGTATAAACTGGCCAGTATGATAAAGCGTGGTAAGAGTACTACGGATGAGATTAGTACACCCTTTGGTATCCGTACCATCAGTTGGCCGGTGAAACGGGAGGATGGTGACGGACGGTTCTATCTGAACGGACAGCCTGTCTTTATCAATGGAGTGTGTGAATATGAACATCAGTTCGGACAAAGCCATGCTTTCAGCCGTGAGCAGGTGGCAGCCCGTGTGAAACAGATCCGTGCGGCGGGCTTCAACGCTTTCCGTGACGCTCACCAGCCCCATCACCTGGATTATCAGAAATATTGGGACAAGGAAGGAGTCTTGTGGTGGACTCAGTTTTCGGCCCACGTGTGGTATGATACTCCGGAGTTTCGTGAGAATTTCAAGAAACTGCTCCGCCAGTGGGTGAAAGAGCGTCGTAACTCGCCCAGTGTGGTGATGTGGGGATTGCAGAACGAAAGCACGCTGCCTAAGGAGTTTGCCGAAGAGTGTAGTGAGATTATCCGGGAAATGGATCCTACGGCCCGTACCATGCGTGTGATTACTACGTGTAACGGTGGTGATGGGACAGATTGGAATGTTATTCAGAACTGGAGTGGAACCTATGGTGGTGATGTGAATAAGTATGGCCGTGAATTGTCACAGAAGAATCAGTTGTTGAACGGTGAATATGGGGCATGGCGCAGCATCGGATTGCATACGGAACCGGCAGCTTTTGATGTGAATGGTGTATGGAGCGAGGAGCGTATGTGCCAGCTGATGGAAACCAAGATACGACTGGCGGAACAGGCAAAGGACAGTGTGTGCGGACAATTCCAATGGATATTCAGCAGTCATGATAATCCGGGACGCCGCCAGCCGGATGAAGCGTATCGCCGGATAGATAAGGTGGGACCATTTAATTATAAAGGATTGGTGACTCCGTGGGAGGAGCCGCTGGATGTATATTATATGTATCGCGCGAACTATGTGCCGGCTTCCGAAGATCCGATGGTTTATCTGGCATCTCATACATGGGAGGACCGTTTTGCGACAGGCCGCAGACGGGCTACCATTGAGGCGTACAGCAATTGTGACAGTGTGTTGCTGTACAATGATGCGGTGGATGCTGAATACTTGGGACGGAAATTGAATCATGGGGTGGGGACTCATTTCATGTGGGAAAACCGCGATATCCGTTACAATGTGCTTCGTGCTGTGGGGTACTTTAAAGGAAAACCGGCAGCGGAAGATGTGCTGGTGCTGAACGGTCTGGAAAAAGCTCCTCATTTTGAAGCTCTTTACCGTGGTTCTGCTATAGTGCCTGTTGCTGCCGACCGGTTGAACGGAACTGATTTGTTGAAAGGAGCTGAGGGATATACGTATCTTTATCGTTTGAATTGCGGAGGAGACGCTTATACGGATACGTATGGTCAGGTCTGGGCGCAGGATAACAGCCGTTATTCTCATTCGTGGGCGGAATCGTTTGTTCATCCGTCGGATAGTGTACAGTTGTTGAGCCCTTATCAGGCGAGCCAGCGTACCACGAATGATCCCATTCATGGCACTAGGGACTGGGAACTGTTCCAGACTTTCCGCTTCGGACGCCATAAGCTGAACTTCCGCTTTCCCGTTCCGGATGGCGAGTATCGTGTGGAACTTTATTTTACAGAACCCTGGCATGGTACAGGAGGCGGCGTACAGACGGATTGTGAAGGCTTGCGTATTTTTGATGTAGCGGTAAATGATAAAGTACTTCTGGACGACTTGGATGTATGGGCTGAAGCGGGTCATGACGGCGCTTGCAAAAAGGTGGTGAATGCTATCGTGAAAGGGGGAGTCTTGAAGATTAATTTTCCGGAGGTGAAAGCGGGGCAAGCTTTGATTTGTGGCATTGCGATAGCTTGTAAAGGCGACTTGGATTCCGTTCGCTCTTTTTCTGCCCATAGCTTCAGCTGGGCTGCACAAGACAAGGAGGTGATGGAAAAGACTCCCAAAGAATTATTGCCTGAAGATAAAAACGCACGTGCCAATGTGACTTATCAAGCGGAGGATGCTGTGCTGAAAGGTAAGTTCATAAAGAAAGAGGTTAAGAAACAAACCGGGGTTTTCTTTGGTAAAGGTACACAAAGCAGTATTACTTGGAACATTTCTACCGGTCTGGCACAAGTCTATGCTCTTCGTTTTAAATACATGAATGTTACAGGGAAGCCTATGAAGGTGCGTATGCAGTTCATTGATTCCAAAGGGGTGGTACTGAAGGAAGATAATCTGACTTTTGCCGAAACTCCCGGAAAGTGGCGTATGCTGAGTACAACGACGGGTACGTATATCAATGCAGGCTATTATAAGGTGGTGCTTTCCGCACCGGATATGGAGGGACTGGCATTGGATGCGCTGGATGTGCAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 6,722 total letters Query= CMR200062.108 Length=4620 Score E Sequences producing significant alignments: (Bits) Value 0_wt 7593 0.0 2_06E_E6_CMR200062.108_T7S.seq 1851 0.0 1_03E_E6_CMR200062.108_T7term.seq 1810 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 58 TTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGG 147 2 9 .......................................................................................... 98 S H P Q F E K G T E N L Y F Q S N A Q R E V P Q S R M E Q I Query_1 148 TCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAACGTGAAGTTCCGCAATCGCGCATGGAGCAGATT 237 0 1 .................................... 36 2 99 .......................................................................................... 188 Y E E A K T P Y K Y G L A V A P A D N K H K I D C P T V F R Query_1 238 TATGAGGAAGCCAAGACTCCTTATAAATATGGGCTGGCTGTAGCTCCGGCCGACAACAAGCATAAAATAGACTGCCCTACCGTGTTCCGC 327 0 37 .......................................................................................... 126 2 189 .......................................................................................... 278 E G D K W Y M T Y V V Y N G K S G L D G R G Y E T W I A E S Query_1 328 GAAGGGGATAAGTGGTATATGACCTACGTGGTTTATAATGGTAAGAGCGGACTGGACGGACGTGGATACGAGACATGGATAGCCGAAAGT 417 0 127 .......................................................................................... 216 2 279 .......................................................................................... 368 D N L L E W R T L G R V L S Y R D G F W D C N Q R G G F P A Query_1 418 GATAATCTGTTGGAATGGCGTACATTGGGACGTGTCTTGTCTTATCGTGACGGATTCTGGGATTGCAACCAGCGTGGTGGTTTCCCTGCA 507 0 217 .......................................................................................... 306 0 4159 ......C.....--... 4173 2 369 .......................................................................................... 458 1 188 ......C.....--... 174 L P D M E W G G S Y A L Q T Y K G K H W M T Y L G G E G T G Query_1 508 TTGCCGGATATGGAGTGGGGAGGAAGCTATGCCCTGCAGACGTATAAAGGCAAGCATTGGATGACTTATCTGGGTGGGGAAGGGACCGGA 597 0 307 .......................................................................................... 396 0 4174 ---............ 4185 2 459 .......................................................................................... 548 1 173 ---............ 162 Y E S V N K P L Y I G L A W T D R P L G S A H E W Q A Q D Q Query_1 598 TATGAATCGGTGAACAAGCCGCTGTATATTGGTTTGGCCTGGACAGACCGGCCTTTGGGTTCCGCCCATGAATGGCAGGCACAGGACCAG 687 0 397 .......................................................................................... 486 2 549 .......................................................................................... 638 P V M S I H D K D A Q W W E K L T Q Y K S V V Y W D K E K Query_1 688 CCCGTGATGAGTATCCATGATAAAGACGCGCAATGGTGGGAAAAGCTGACTCAATATAAGAGTGTGGTCTATTGGGAT-AAGGAGAAAAC 776 0 487 ..............................................................................-........... 575 2 639 ..............................................................................A........... 728 T L G A P F V M F Y N A A G R H P E T D L K A E R V G I A L Query_1 777 ATTGGGCGCACCTTTCGTCATGTTCTATAACGCGGCAGGACGGCATCCGGAAACGGATTTGAAAGCGGAACGGGTGGGTATCGCCTTGTC 866 0 576 .......................................................................................... 665 0 1448 ............ 1459 2 729 ...................................N.....................................................N 818 S K D M K K W K R Y P G N P V F A H E A D G T I T G D A H I Query_1 867 CAAAGATATGAAGAAATGGAAACGATATCCGGGCAATCCGGTCTTTGCACACGAAGCGGACGGAACCATTACGGGGGATGCGCATAT-CC 955 0 666 .......................................................................................-.. 754 2 819 .................................................................................N.....C.. 908 Q K M G D V Y V M F Y F S A F E P S R K Y K A F N T F A A Query_1 956 AGAAAA-TGGGCGATGTGTATGTCATGTTCTATTTC-TCGGCTTTTGAACCGAGCCGCAAATACAAGGCATTTAATACATTTGC-TGCCA 1042 0 755 ......-.............................-...............................................-..... 841 0 592 ............AA.-G.... 611 2 909 ......T.......N............-........N.....-...........T...T..............-..........N....N 995 2 745 ............ 756 S Y D L V H W T D W K G A D L I I P S K D Y D E L F A H K S Query_1 1043 GTTATGACCTAGTACATTGGACGGATTGGAAAGGGGCGGATTTGATTATCCCTTCTAAAGACTATGATGAATTGTTCGCTCATAAGAG-T 1131 0 842 ........................................................................................-. 930 2 996 ............N............-..............-......-.T..............................NC...AN.T. 1082 Y V V K Y N G V V Y H F Y C A V N D A E Q R G I A I A T S Query_1 1132 TATGTGGTGAAATACAATGGGGTGGTGTATCATT-TCTATTGTGCCGTGAATGACGCGGAACAACGTGGTATAG-CCATTGCTACCAGCA 1219 0 931 ..................................-.......................................-............... 1018 2 1083 .....CNNNN...NN.T...N....NNG......C..AT.G............NN..-N.......N..NN...C........-N...GC 1170 K P M G R S Q V H F P E R E V K N R R M V M E L D K G W K T Query_1 1220 AACCGATGGGA-CGTTCACAAGTGCATTTCCCGGAACGGGAGGTGAAGAATCGCCGGATGGTGATGGAATTGGATAAAGGATGGAAAACA 1308 0 1019 ...........-.............................................................................. 1107 2 1171 ........T..N.... 1186 W L T E A T H L K G L F A Q K A I E V N I P H N W D D Y Y G Query_1 1309 TGGCTGACGGAAGCAACTCATCTGAAAGGGTTGTTTGCTCAAAAGGCAATAGAAGTAAATATTCCTCATAACTGGGATGATTATTATGGC 1398 0 1108 .......................................................................................... 1197 Y R Q L T H G N L H G T A I Y E K T F T L D D S Q F L I S N Query_1 1399 TACCGCCAACTGACACATGGTAATTTGCACGGTACTGCCATATACGAAAAGACCTTCACATTGGATGATTCTCAGTTTCTAATTTCCAAC 1488 0 1198 .......................................................................................... 1287 S S F G K R Y F L R F E G V G T Y A T I T L N G K D F G R H Query_1 1489 TCTTCATTCGGAAAACGTTATTTTCTTCGTTTTGAGGGAGTGGGGACGTATGCCACTATAACGTTGAATGGAAAGGATTTCGGCCGTCAT 1578 0 1288 .......................................................................................... 1377 P V G R T T L T L D V T D A L K L G E N K L V V K A E H P E Query_1 1579 CCCGTAGGGCGTACTACGCTGACATTGGATGTGACGGATGCGTTGAAGCTGGGAGAGAATAAGCTGGTAGTGAAAGCGGAACATCCTGAA 1668 0 1378 .......................................................................................... 1467 2 788 ............ 799 M I A D M P W V C G G C S S E W G F S E G S Q P L G I F R P Query_1 1669 ATGATAGCCGACATGCCTTGGGTGTGCGGTGGCTGTTCCTCGGAATGGGGATTCAGCGAAGGTTCCCAGCCTTTGGGAATTTTCCGTCCC 1758 0 1468 .......................................................................................... 1557 V V L E A T D E I R I E P F G V H I W N D D K A G T V F V E Query_1 1759 GTGGTGCTGGAGGCTACGGATGAGATCCGCATTGAGCCTTTCGGAGTGCATATCTGGAATGATGATAAGGCGGGAACTGTCTTTGTGGAA 1848 0 1558 .......................................................................................... 1647 0 1874 ............. 1886 T E V K N Y G K T A E T V E V V N K F S N A D G K Q V F R L Query_1 1849 ACGGAGGTGAAAAATTACGGAAAGACTGCTGAGACGGTGGAAGTGGTTAATAAATTCAGTAATGCGGATGGCAAGCAGGTGTTCCGCCTG 1938 0 1648 .......................................................................................... 1737 T E K V T L Q P G E K K V V K Q Q S P V Q N P V L W S T E N Query_1 1939 ACGGAGAAGGTGACTTTGCAGCCGGGTGAAAAGAAGGTGGTGAAGCAACAATCGCCAGTGCAGAATCCTGTTTTATGGAGTACGGAGAAT 2028 0 1738 .......................................................................................... 1827 P Y L Y K L A S M I K R G K S T T D E I S T P F G I R T I S Query_1 2029 CCTTATTTGTATAAACTGGCCAGTATGATAAAGCGTGGTAAGAGTACTACGGATGAGATTAGTACACCCTTTGGTATCCGTACCATCAGT 2118 0 1828 .......................................................................................... 1917 0 1571 ............. 1583 W P V K R E D G D G R F Y L N G Q P V F I N G V C E Y E H Q Query_1 2119 TGGCCGGTGAAACGGGAGGATGGTGACGGACGGTTCTATCTGAACGGACAGCCTGTCTTTATCAATGGAGTGTGTGAATATGAACATCAG 2208 0 1918 .......................................................................................... 2007 F G Q S H A F S R E Q V A A R V K Q I R A A G F N A F R D A Query_1 2209 TTCGGACAAAGCCATGCTTTCAGCCGTGAGCAGGTGGCAGCCCGTGTGAAACAGATCCGTGCGGCGGGCTTCAACGCTTTCCGTGACGCT 2298 0 2008 .......................................................................................... 2097 H Q P H H L D Y Q K Y W D K E G V L W W T Q F S A H V W Y D Query_1 2299 CACCAGCCCCATCACCTGGATTATCAGAAATATTGGGACAAGGAAGGAGTCTTGTGGTGGACTCAGTTTTCGGCCCACGTGTGGTATGAT 2388 0 2098 .......................................................................................... 2187 T P E F R E N F K K L L R Q W V K E R R N S P S V V M W G L Query_1 2389 ACTCCGGAGTTTCGTGAGAATTTCAAGAAACTGCTCCGCCAGTGGGTGAAAGAGCGTCGTAACTCGCCCAGTGTGGTGATGTGGGGATTG 2478 0 2188 .......................................................................................... 2277 Q N Q S T L P K E F A E E C S E I I R E M D P T A R T M R V Query_1 2479 CAGAACCAGAGCACGCTGCCTAAGGAGTTTGCCGAAGAGTGTAGTGAGATTATCCGGGAAATGGATCCTACGGCCCGTACCATGCGTGTG 2568 0 2278 ......G.A................................................................................. 2367 I T T C N G G D G T D W N V I Q N W S G T Y G G D V N K Y G Query_1 2569 ATTACTACGTGTAACGGTGGTGATGGGACAGATTGGAATGTTATTCAGAACTGGAGTGGAACCTATGGTGGTGATGTGAATAAGTATGGC 2658 0 2368 .......................................................................................... 2457 R E L S Q K N Q L L N G E Y G A W R S I G L H T E P A A F D Query_1 2659 CGTGAATTGTCACAGAAGAATCAGTTGTTGAACGGTGAATATGGGGCATGGCGCAGCATCGGATTGCATACGGAACCGGCAGCTTTTGAT 2748 0 2458 .......................................................................................... 2547 V N G V W S E E R M C Q L M E T K I R L A E Q A K D S V C G Query_1 2749 GTGAATGGTGTATGGAGCGAGGAGCGTATGTGCCAGCTGATGGAAACCAAGATACGACTGGCGGAACAGGCAAAGGACAGTGTGTGCGGA 2838 0 2548 .......................................................................................... 2637 Q F Q W I F S S H D N P G R R Q P D E A Y R R I D K V G P F Query_1 2839 CAATTCCAATGGATATTCAGCAGTCATGATAATCCGGGACGCCGCCAGCCGGATGAAGCGTATCGCCGGATAGATAAGGTGGGACCATTT 2928 0 2638 .......................................................................................... 2727 N Y K G L V T P W E E P L D V Y Y M Y R A N Y V P A S E D P Query_1 2929 AATTATAAAGGATTGGTGACTCCGTGGGAGGAGCCGCTGGATGTATATTATATGTATCGCGCGAACTATGTGCCGGCTTCCGAAGATCCG 3018 0 2728 .......................................................................................... 2817 M V Y L A S H T W E D R F A T G R R R A T I E A Y S N C D S Query_1 3019 ATGGTTTATCTGGCATCTCATACATGGGAGGACCGTTTTGCGACAGGCCGCAGACGGGCTACCATTGAGGCGTACAGCAATTGTGACAGT 3108 0 2818 .......................................................................................... 2907 V L L Y N D A V D A E Y L G R K L N H G V G T H F M W E N R Query_1 3109 GTGTTGCTGTACAATGATGCGGTGGATGCTGAATACTTGGGACGGAAATTGAATCATGGGGTGGGGACTCATTTCATGTGGGAAAACCGC 3198 0 2908 .......................................................................................... 2997 D I R Y N V L R A V G Y F K G K P A A E D V L V L N G L E K Query_1 3199 GATATCCGTTACAATGTGCTTCGTGCTGTGGGGTACTTTAAAGGAAAACCGGCAGCGGAAGATGTGCTGGTGCTGAACGGTCTGGAAAAA 3288 0 2998 .......................................................................................... 3087 A P H F E A L Y R G S A I V P V A A D R L N G T D L L K G A Query_1 3289 GCTCCTCATTTTGAAGCTCTTTACCGTGGTTCTGCTATAGTGCCTGTTGCTGCCGACCGGTTGAACGGAACTGATTTGTTGAAAGGAGCT 3378 0 3088 .......................................................................................... 3177 E G Y T Y L Y R L N C G G D A Y T D T Y G Q V W A Q D Query_1 3379 GAGGGATATACGTATCTTTATCGTTTGAA-TTGCGGAGGAGA---CGCTTATACGGATAC-GTATGGTC-AGGTCTGG-GCGCAGGA-TA 3460 0 3178 .............................-............---...............-........-........-........-.. 3259 1 1169 ...AN...N.....A.........G..GGA....A.N.....NNNN.N.N....C........AN....NN.A.. 1095 N S R Y S H S W A E S F V H P S D S V Q L L S P Y Q A S Query_1 3461 A--CAGCCGTTATTCTCATTCGTGGGCGG--AATCG-TTTGTTCATCCGTCGGATAGTGTACAGTTGTTGA-GCCCTTATCAGGC-GAGC 3543 0 3260 .--..........................--.....-..................................-.............-.... 3342 1 1094 .AC.......-...N...NNN-...NN..GA.....G....N................NNNA.....G...A.....AN......N.... 1007 Q R T T N D P I H G T R D W E L F Q T F R F G R H K L N F Query_1 3544 CA-GCGTACCACGAATGATCCCATTCATGGCACTAGGGACTGGG-AACTGTTCCAGACTTTCCGCTTCGGACGCCATAAGCTGAACTT-C 3630 0 3343 ..-.........................................-...........................................-. 3429 1 1006 .NN.........................................G...........N...............................Y. 917 R F P V P D G E Y R V E L Y F T E P W H G T G G G V Q T D Query_1 3631 CGCTTTCCCGTTCCGGATGGCGA-GTATCGTGTGGAACTTTATTTTACAGAACCCTGGCATGGTACAGGAGGCGGCGTACAGACGGATTG 3719 0 3430 .......................-.................................................................. 3518 1 916 ...............-.......A.................................................................. 828 C E G L R I F D V A V N D K V L L D D L D V W A E A G H D G Query_1 3720 TGAAGGCTTGCGTATTTTTGATGTAGCGGTAAATGATAAAGTACTTCTGGACGACTTGGATGTATGGGCTGAAGCGGGTCATGACGGCGC 3809 0 3519 .......................................................................................... 3608 1 827 .................................................-........................................ 739 A C K K V V N A I V K G G V L K I N F P E V K A G Q A L I C Query_1 3810 TTGCAAAAAGGTGGTGAATGCTATCGTGAAAGGGGGAGTCTTGAAGATTAATTTTCCGGAGGTGAAAGCGGGGCAAGCTTTGATTTGTGG 3899 0 3609 .......................................................................................... 3698 1 738 .......................................................................................... 649 G I A I A C K G D L D S V R S F S A H S F S W A A Q D K E V Query_1 3900 CATTGCGATAGCTTGTAAAGGCGACTTGGATTCCGTTCGCTCTTTTTCTGCCCATAGCTTCAGCTGGGCTGCACAAGACAAGGAGGTGAT 3989 0 3699 .......................................................................................... 3788 1 648 .......................................................................................... 559 M E K T P K E L L P E D K N A R A N V T Y Q A E D A V L K G Query_1 3990 GGAAAAGACTCCCAAAGAATTATTGCCTGAAGATAAAAACGCACGTGCCAATGTGACTTATCAAGCGGAGGATGCTGTGCTGAAAGGTAA 4079 0 3789 .......................................................................................... 3878 1 558 .......................................................................................... 469 K F I K K E V K K Q T G V F F G K G T Q S S I T W N I S T G Query_1 4080 GTTCATAAAGAAAGAGGTTAAGAAACAAACCGGGGTTTTCTTTGGTAAAGGTACACAAAGCAGTATTACTTGGAACATTTCTACCGGTCT 4169 0 3879 .......................................................................................... 3968 1 468 .......................................................................................... 379 L A Q V Y A L R F K Y M N V T G K P M K V R M Q F I D S K G Query_1 4170 GGCACAAGTCTATGCTCTTCGTTTTAAATACATGAATGTTACAGGGAAGCCTATGAAGGTGCGTATGCAGTTCATTGATTCCAAAGGGGT 4259 0 3969 .......................................................................................... 4058 1 378 .......................................................................................... 289 V V L K E D N L T F A E T P G K W R M L S T T T G T Y I N A Query_1 4260 GGTACTGAAGGAAGATAATCTGACTTTTGCCGAAACTCCCGGAAAGTGGCGTATGCTGAGTACAACGACGGGTACGTATATCAATGCAGG 4349 0 4059 .......................................................................................... 4148 1 288 .......................................................................................... 199 G Y Y K V V L S A P D M E G L A L D A L D V Q * H W K W I Query_1 4350 CTATTATAAGGTGGTGCTTTCC--GCA---CCGGATATGGAGGGACTGGCATTGGATGCGCTGGATGTGCAATAACATTGGAAGTGGATA 4434 0 4149 ......................--...---.......................................... 4215 0 290 ......G.....CT...TTG............ 321 2 442 ......G.....CT...TTG............ 473 1 198 ......................--...---............................................................ 114 T D P N S S S V D K L A A A L E H H H H H H * D P A A N K A Query_1 4435 ACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCC 4524 1 113 .......................................................................................... 24 R K E Query_1 4525 CGAAAGGAAG 4534 1 23 .......... 14 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 30686136 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 6,722 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200062.109 (CMR200062.19-1423.P(1423).E850Q.pMCSG68)
QREVPQSRMEQIYEEAKTPYKYGLAVAPADNKHKIDCPTVFREGDKWYMTYVVYNGKSGLDGRGYETWIAESDNLLEWRTLGRVLSYRDGFWDCNQRGGFPALPDMEWGGSYALQTYKGKHWMTYLGGEGTGYESVNKPLYIGLAWTDRPLGSAHEWQAQDQPVMSIHDKDAQWWEKLTQYKSVVYWDKEKTLGAPFVMFYNAAGRHPETDLKAERVGIALSKDMKKWKRYPGNPVFAHEADGTITGDAHIQKMGDVYVMFYFSAFEPSRKYKAFNTFAASYDLVHWTDWKGADLIIPSKDYDELFAHKSYVVKYNGVVYHFYCAVNDAEQRGIAIATSKPMGRSQVHFPEREVKNRRMVMELDKGWKTWLTEATHLKGLFAQKAIEVNIPHNWDDYYGYRQLTHGNLHGTAIYEKTFTLDDSQFLISNSSFGKRYFLRFEGVGTYATITLNGKDFGRHPVGRTTLTLDVTDALKLGENKLVVKAEHPEMIADMPWVCGGCSSEWGFSEGSQPLGIFRPVVLEATDEIRIEPFGVHIWNDDKAGTVFVETEVKNYGKTAETVEVVNKFSNADGKQVFRLTEKVTLQPGEKKVVKQQSPVQNPVLWSTENPYLYKLASMIKRGKSTTDEISTPFGIRTISWPVKREDGDGRFYLNGQPVFINGVCEYEHQFGQSHAFSREQVAARVKQIRAAGFNAFRDAHQPHHLDYQKYWDKEGVLWWTQFSAHVWYDTPEFRENFKKLLRQWVKERRNSPSVVMWGLQNESTLPKEFAEECSEIIREMDPTARTMRVITTCNGGDGTDWNVIQNWSGTYGGDVNKYGRELSQKNQLLNGEYGAWRSIGLHTEPAAFDVNGVWSEERMCQLMETKIRLAEQAKDSVCGQFQWIFSSHDNPGRRQPDEAYRRIDKVGPFNYKGLVTPWEEPLDVYYMYRANYVPASEDPMVYLASHTWEDRFATGRRRATIEAYSNCDSVLLYNDAVDAEYLGRKLNHGVGTHFMWENRDIRYNVLRAVGYFKGKPAAEDVLVLNGLEKAPHFEALYRGSAIVPVAADRLNGTDLLKGAEGYTYLYRLNCGGDAYTDTYGQVWAQDNSRYSHSWAESFVHPSDSVQLLSPYQASQRTTNDPIHGTRDWELFQTFRFGRHKLNFRFPVPDGEYRVELYFTEPWHGTGGGVQTDCEGLRIFDVAVNDKVLLDDLDVWAEAGHDGACKKVVNAIVKGGVLKINFPEVKAGQALICGIAIACKGDLDSVRSFSAHSFSWAAQDKEVMEKTPKELLPEDKNARANVTYQAEDAVLKGKFIKKEVKKQTGVFFGKGTQSSITWNISTGLAQVYALRFKYMNVTGKPMKVRMQFIDSKGVVLKEDNLTFAETPGKWRMLSTTTGTYINAGYYKVVLSAPDMEGLALDALDVQ
CAACGTGAAGTTCCGCAATCGCGCATGGAGCAGATTTATGAGGAAGCCAAGACTCCTTATAAATATGGGCTGGCTGTAGCTCCGGCCGACAACAAGCATAAAATAGACTGCCCTACCGTGTTCCGCGAAGGGGATAAGTGGTATATGACCTACGTGGTTTATAATGGTAAGAGCGGACTGGACGGACGTGGATACGAGACATGGATAGCCGAAAGTGATAATCTGTTGGAATGGCGTACATTGGGACGTGTCTTGTCTTATCGTGACGGATTCTGGGATTGCAACCAGCGTGGTGGTTTCCCTGCATTGCCGGATATGGAGTGGGGAGGAAGCTATGCCCTGCAGACGTATAAAGGCAAGCATTGGATGACTTATCTGGGTGGGGAAGGGACCGGATATGAATCGGTGAACAAGCCGCTGTATATTGGTTTGGCCTGGACAGACCGGCCTTTGGGTTCCGCCCATGAATGGCAGGCACAGGACCAGCCCGTGATGAGTATCCATGATAAAGACGCGCAATGGTGGGAAAAGCTGACTCAATATAAGAGTGTGGTCTATTGGGATAAGGAGAAAACATTGGGCGCACCTTTCGTCATGTTCTATAACGCGGCAGGACGGCATCCGGAAACGGATTTGAAAGCGGAACGGGTGGGTATCGCCTTGTCCAAAGATATGAAGAAATGGAAACGATATCCGGGCAATCCGGTCTTTGCACACGAAGCGGACGGAACCATTACGGGGGATGCGCATATCCAGAAAATGGGCGATGTGTATGTCATGTTCTATTTCTCGGCTTTTGAACCGAGCCGCAAATACAAGGCATTTAATACATTTGCTGCCAGTTATGACCTAGTACATTGGACGGATTGGAAAGGGGCGGATTTGATTATCCCTTCTAAAGACTATGATGAATTGTTCGCTCATAAGAGTTATGTGGTGAAATACAATGGGGTGGTGTATCATTTCTATTGTGCCGTGAATGACGCGGAACAACGTGGTATAGCCATTGCTACCAGCAAACCGATGGGACGTTCACAAGTGCATTTCCCGGAACGGGAGGTGAAGAATCGCCGGATGGTGATGGAATTGGATAAAGGATGGAAAACATGGCTGACGGAAGCAACTCATCTGAAAGGGTTGTTTGCTCAAAAGGCAATAGAAGTAAATATTCCTCATAACTGGGATGATTATTATGGCTACCGCCAACTGACACATGGTAATTTGCACGGTACTGCCATATACGAAAAGACCTTCACATTGGATGATTCTCAGTTTCTAATTTCCAACTCTTCATTCGGAAAACGTTATTTTCTTCGTTTTGAGGGAGTGGGGACGTATGCCACTATAACGTTGAATGGAAAGGATTTCGGCCGTCATCCCGTAGGGCGTACTACGCTGACATTGGATGTGACGGATGCGTTGAAGCTGGGAGAGAATAAGCTGGTAGTGAAAGCGGAACATCCTGAAATGATAGCCGACATGCCTTGGGTGTGCGGTGGCTGTTCCTCGGAATGGGGATTCAGCGAAGGTTCCCAGCCTTTGGGAATTTTCCGTCCCGTGGTGCTGGAGGCTACGGATGAGATCCGCATTGAGCCTTTCGGAGTGCATATCTGGAATGATGATAAGGCGGGAACTGTCTTTGTGGAAACGGAGGTGAAAAATTACGGAAAGACTGCTGAGACGGTGGAAGTGGTTAATAAATTCAGTAATGCGGATGGCAAGCAGGTGTTCCGCCTGACGGAGAAGGTGACTTTGCAGCCGGGTGAAAAGAAGGTGGTGAAGCAACAATCGCCAGTGCAGAATCCTGTTTTATGGAGTACGGAGAATCCTTATTTGTATAAACTGGCCAGTATGATAAAGCGTGGTAAGAGTACTACGGATGAGATTAGTACACCCTTTGGTATCCGTACCATCAGTTGGCCGGTGAAACGGGAGGATGGTGACGGACGGTTCTATCTGAACGGACAGCCTGTCTTTATCAATGGAGTGTGTGAATATGAACATCAGTTCGGACAAAGCCATGCTTTCAGCCGTGAGCAGGTGGCAGCCCGTGTGAAACAGATCCGTGCGGCGGGCTTCAACGCTTTCCGTGACGCTCACCAGCCCCATCACCTGGATTATCAGAAATATTGGGACAAGGAAGGAGTCTTGTGGTGGACTCAGTTTTCGGCCCACGTGTGGTATGATACTCCGGAGTTTCGTGAGAATTTCAAGAAACTGCTCCGCCAGTGGGTGAAAGAGCGTCGTAACTCGCCCAGTGTGGTGATGTGGGGATTGCAGAACGAAAGCACGCTGCCTAAGGAGTTTGCCGAAGAGTGTAGTGAGATTATCCGGGAAATGGATCCTACGGCCCGTACCATGCGTGTGATTACTACGTGTAACGGTGGTGATGGGACAGATTGGAATGTTATTCAGAACTGGAGTGGAACCTATGGTGGTGATGTGAATAAGTATGGCCGTGAATTGTCACAGAAGAATCAGTTGTTGAACGGTGAATATGGGGCATGGCGCAGCATCGGATTGCATACGGAACCGGCAGCTTTTGATGTGAATGGTGTATGGAGCGAGGAGCGTATGTGCCAGCTGATGGAAACCAAGATACGACTGGCGGAACAGGCAAAGGACAGTGTGTGCGGACAATTCCAATGGATATTCAGCAGTCATGATAATCCGGGACGCCGCCAGCCGGATGAAGCGTATCGCCGGATAGATAAGGTGGGACCATTTAATTATAAAGGATTGGTGACTCCGTGGGAGGAGCCGCTGGATGTATATTATATGTATCGCGCGAACTATGTGCCGGCTTCCGAAGATCCGATGGTTTATCTGGCATCTCATACATGGGAGGACCGTTTTGCGACAGGCCGCAGACGGGCTACCATTGAGGCGTACAGCAATTGTGACAGTGTGTTGCTGTACAATGATGCGGTGGATGCTGAATACTTGGGACGGAAATTGAATCATGGGGTGGGGACTCATTTCATGTGGGAAAACCGCGATATCCGTTACAATGTGCTTCGTGCTGTGGGGTACTTTAAAGGAAAACCGGCAGCGGAAGATGTGCTGGTGCTGAACGGTCTGGAAAAAGCTCCTCATTTTGAAGCTCTTTACCGTGGTTCTGCTATAGTGCCTGTTGCTGCCGACCGGTTGAACGGAACTGATTTGTTGAAAGGAGCTGAGGGATATACGTATCTTTATCGTTTGAATTGCGGAGGAGACGCTTATACGGATACGTATGGTCAGGTCTGGGCGCAGGATAACAGCCGTTATTCTCATTCGTGGGCGGAATCGTTTGTTCATCCGTCGGATAGTGTACAGTTGTTGAGCCCTTATCAGGCGAGCCAGCGTACCACGAATGATCCCATTCATGGCACTAGGGACTGGGAACTGTTCCAGACTTTCCGCTTCGGACGCCATAAGCTGAACTTCCGCTTTCCCGTTCCGGATGGCGAGTATCGTGTGGAACTTTATTTTACAGAACCCTGGCATGGTACAGGAGGCGGCGTACAGACGGATTGTGAAGGCTTGCGTATTTTTGATGTAGCGGTAAATGATAAAGTACTTCTGGACGACTTGGATGTATGGGCTGAAGCGGGTCATGACGGCGCTTGCAAAAAGGTGGTGAATGCTATCGTGAAAGGGGGAGTCTTGAAGATTAATTTTCCGGAGGTGAAAGCGGGGCAAGCTTTGATTTGTGGCATTGCGATAGCTTGTAAAGGCGACTTGGATTCCGTTCGCTCTTTTTCTGCCCATAGCTTCAGCTGGGCTGCACAAGACAAGGAGGTGATGGAAAAGACTCCCAAAGAATTATTGCCTGAAGATAAAAACGCACGTGCCAATGTGACTTATCAAGCGGAGGATGCTGTGCTGAAAGGTAAGTTCATAAAGAAAGAGGTTAAGAAACAAACCGGGGTTTTCTTTGGTAAAGGTACACAAAGCAGTATTACTTGGAACATTTCTACCGGTCTGGCACAAGTCTATGCTCTTCGTTTTAAATACATGAATGTTACAGGGAAGCCTATGAAGGTGCGTATGCAGTTCATTGATTCCAAAGGGGTGGTACTGAAGGAAGATAATCTGACTTTTGCCGAAACTCCCGGAAAGTGGCGTATGCTGAGTACAACGACGGGTACGTATATCAATGCAGGCTATTATAAGGTGGTGCTTTCCGCACCGGATATGGAGGGACTGGCATTGGATGCGCTGGATGTGCAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 6,699 total letters Query= CMR200062.109 Length=4620 Score E Sequences producing significant alignments: (Bits) Value 0_wt 7593 0.0 2_06F_F6_CMR200062.109_T7S.seq 1905 0.0 1_03F_F6_CMR200062.109_T7term.seq 1902 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 60 CCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTC 149 2 12 .......................................................................................... 101 S H P Q F E K G T E N L Y F Q S N A Q R E V P Q S R M E Q I Query_1 150 TCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAACGTGAAGTTCCGCAATCGCGCATGGAGCAGATTTA 239 0 1 ...................................... 38 2 102 .......................................................................................... 191 Y E E A K T P Y K Y G L A V A P A D N K H K I D C P T V F R Query_1 240 TGAGGAAGCCAAGACTCCTTATAAATATGGGCTGGCTGTAGCTCCGGCCGACAACAAGCATAAAATAGACTGCCCTACCGTGTTCCGCGA 329 0 39 .......................................................................................... 128 2 192 .......................................................................................... 281 E G D K W Y M T Y V V Y N G K S G L D G R G Y E T W I A E S Query_1 330 AGGGGATAAGTGGTATATGACCTACGTGGTTTATAATGGTAAGAGCGGACTGGACGGACGTGGATACGAGACATGGATAGCCGAAAGTGA 419 0 129 .......................................................................................... 218 2 282 .......................................................................................... 371 D N L L E W R T L G R V L S Y R D G F W D C N Q R G G F P A Query_1 420 TAATCTGTTGGAATGGCGTACATTGGGACGTGTCTTGTCTTATCGTGACGGATTCTGGGATTGCAACCAGCGTGGTGGTTTCCCTGCATT 509 0 219 .......................................................................................... 308 0 4159 ......C.....--...-- 4173 2 372 .......................................................................................... 461 1 188 ......C.....--...-- 174 L P D M E W G G S Y A L Q T Y K G K H W M T Y L G G E G T G Query_1 510 GCCGGATATGGAGTGGGGAGGAAGCTATGCCCTGCAGACGTATAAAGGCAAGCATTGGATGACTTATCTGGGTGGGGAAGGGACCGGATA 599 0 309 .......................................................................................... 398 0 4174 -............ 4185 2 462 .......................................................................................... 551 1 173 -............ 162 Y E S V N K P L Y I G L A W T D R P L G S A H E W Q A Q D Q Query_1 600 TGAATCGGTGAACAAGCCGCTGTATATTGGTTTGGCCTGGACAGACCGGCCTTTGGGTTCCGCCCATGAATGGCAGGCACAGGACCAGCC 689 0 399 .......................................................................................... 488 2 552 .......................................................................................... 641 P V M S I H D K D A Q W W E K L T Q Y K S V V Y W D K E K T Query_1 690 CGTGATGAGTATCCATGATAAAGACGCGCAATGGTGGGAAAAGCTGACTCAATATAAGAGTGTGGTCTATTGGGATAAGGAGAAAACATT 779 0 489 .......................................................................................... 578 2 642 .......................................................................................... 731 L G A P F V M F Y N A A G R H P E T D L K A E R V G I A L S Query_1 780 GGGCGCACCTTTCGTCATGTTCTATAACGCGGCAGGACGGCATCCGGAAACGGATTTGAAAGCGGAACGGGTGGGTATCGCCTTGTCCAA 869 0 579 .......................................................................................... 668 0 1448 ............ 1459 2 732 .......................................................................................... 821 2 930 ............TN.T.... 949 K D M K K W K R Y P G N P V F A H E A D G T I T G D A H I Q Query_1 870 AGATATGAAGAAATGG-AAACGATATCCGGGCAATCCGGTCTTTGCACACGAAGCGGACGGAACCATTACGGGGGATGCGCATA-TCCAG 957 0 669 ................-...................................................................-..... 756 2 822 ................A.....................................................N.............T..... 911 K M G D V Y V M F Y F S A F E P S R K Y K A F N T F A A S Query_1 958 AAAATGGGCGATGTGTATGTCATGTTCTATTTCTCGGCTTTTGAACCGAGCCGCAAA-TACAAGGCATTTAATACA-TTTGCTGCCAGTT 1045 0 757 .........................................................-..................-............. 844 0 592 ............AA.G.... 611 2 912 ...............................N......-........N.........N................N.T...........-. 999 2 745 ............AA.G.... 764 Y D L V H W T D W K G A D L I I P S K D Y D E L F A H K S Y Query_1 1046 ATGACCTAGTACATTGGACGGATTGGAAAGGGGCGGATTTGATTATCCCTTCTAAAGACTATGATGAATTGTTCGCTCATAAGAGTTATG 1135 0 845 .......................................................................................... 934 2 1000 .............................N.......-..N.....-......-.................-..N...-N..N.N..... 1084 V V K Y N G V V Y H F Y C A V N D A E Q R G I A I A T S K Query_1 1136 TGGTGAAATACAATGGGGTGGTGTATC-ATTTCTATT--GTGCCGTGAATGACGCGGAACAACGTGGTATAGCCATTGCTACCAGCAAAC 1222 0 935 ...........................-.........--................................................... 1021 2 1085 ........N......N..-.NNN....N.........TN.G......N....NC..C.........NNNANNN......N.....GC... 1173 P M G R S Q V H F P E R E V K N R R M V M E L D K G W K T W Query_1 1223 CGA-TGGGACGTTCACAAGTGCATTTCCCGGAACGGGAGGTGAAGAATCGCCGGATGGTGATGGAATTGGATAAAGGATGGAAAACATGG 1311 0 1022 ...-...................................................................................... 1110 2 1174 ...T...N.N....N.......... 1198 L T E A T H L K G L F A Q K A I E V N I P H N W D D Y Y G Y Query_1 1312 CTGACGGAAGCAACTCATCTGAAAGGGTTGTTTGCTCAAAAGGCAATAGAAGTAAATATTCCTCATAACTGGGATGATTATTATGGCTAC 1401 0 1111 .......................................................................................... 1200 R Q L T H G N L H G T A I Y E K T F T L D D S Q F L I S N S Query_1 1402 CGCCAACTGACACATGGTAATTTGCACGGTACTGCCATATACGAAAAGACCTTCACATTGGATGATTCTCAGTTTCTAATTTCCAACTCT 1491 0 1201 .......................................................................................... 1290 S F G K R Y F L R F E G V G T Y A T I T L N G K D F G R H P Query_1 1492 TCATTCGGAAAACGTTATTTTCTTCGTTTTGAGGGAGTGGGGACGTATGCCACTATAACGTTGAATGGAAAGGATTTCGGCCGTCATCCC 1581 0 1291 .......................................................................................... 1380 V G R T T L T L D V T D A L K L G E N K L V V K A E H P E M Query_1 1582 GTAGGGCGTACTACGCTGACATTGGATGTGACGGATGCGTTGAAGCTGGGAGAGAATAAGCTGGTAGTGAAAGCGGAACATCCTGAAATG 1671 0 1381 .......................................................................................... 1470 2 788 ............ 799 I A D M P W V C G G C S S E W G F S E G S Q P L G I F R P V Query_1 1672 ATAGCCGACATGCCTTGGGTGTGCGGTGGCTGTTCCTCGGAATGGGGATTCAGCGAAGGTTCCCAGCCTTTGGGAATTTTCCGTCCCGTG 1761 0 1471 .......................................................................................... 1560 V L E A T D E I R I E P F G V H I W N D D K A G T V F V E T Query_1 1762 GTGCTGGAGGCTACGGATGAGATCCGCATTGAGCCTTTCGGAGTGCATATCTGGAATGATGATAAGGCGGGAACTGTCTTTGTGGAAACG 1851 0 1561 .......................................................................................... 1650 0 1874 ............. 1886 E V K N Y G K T A E T V E V V N K F S N A D G K Q V F R L T Query_1 1852 GAGGTGAAAAATTACGGAAAGACTGCTGAGACGGTGGAAGTGGTTAATAAATTCAGTAATGCGGATGGCAAGCAGGTGTTCCGCCTGACG 1941 0 1651 .......................................................................................... 1740 E K V T L Q P G E K K V V K Q Q S P V Q N P V L W S T E N P Query_1 1942 GAGAAGGTGACTTTGCAGCCGGGTGAAAAGAAGGTGGTGAAGCAACAATCGCCAGTGCAGAATCCTGTTTTATGGAGTACGGAGAATCCT 2031 0 1741 .......................................................................................... 1830 Y L Y K L A S M I K R G K S T T D E I S T P F G I R T I S W Query_1 2032 TATTTGTATAAACTGGCCAGTATGATAAAGCGTGGTAAGAGTACTACGGATGAGATTAGTACACCCTTTGGTATCCGTACCATCAGTTGG 2121 0 1831 .......................................................................................... 1920 0 1571 ............. 1583 P V K R E D G D G R F Y L N G Q P V F I N G V C E Y E H Q F Query_1 2122 CCGGTGAAACGGGAGGATGGTGACGGACGGTTCTATCTGAACGGACAGCCTGTCTTTATCAATGGAGTGTGTGAATATGAACATCAGTTC 2211 0 1921 .......................................................................................... 2010 G Q S H A F S R E Q V A A R V K Q I R A A G F N A F R D A H Query_1 2212 GGACAAAGCCATGCTTTCAGCCGTGAGCAGGTGGCAGCCCGTGTGAAACAGATCCGTGCGGCGGGCTTCAACGCTTTCCGTGACGCTCAC 2301 0 2011 .......................................................................................... 2100 Q P H H L D Y Q K Y W D K E G V L W W T Q F S A H V W Y D T Query_1 2302 CAGCCCCATCACCTGGATTATCAGAAATATTGGGACAAGGAAGGAGTCTTGTGGTGGACTCAGTTTTCGGCCCACGTGTGGTATGATACT 2391 0 2101 .......................................................................................... 2190 P E F R E N F K K L L R Q W V K E R R N S P S V V M W G L Q Query_1 2392 CCGGAGTTTCGTGAGAATTTCAAGAAACTGCTCCGCCAGTGGGTGAAAGAGCGTCGTAACTCGCCCAGTGTGGTGATGTGGGGATTGCAG 2481 0 2191 .......................................................................................... 2280 N E S T L P K E F A E E C S E I I R E M D P T A R T M R V I Query_1 2482 AACGAAAGCACGCTGCCTAAGGAGTTTGCCGAAGAGTGTAGTGAGATTATCCGGGAAATGGATCCTACGGCCCGTACCATGCGTGTGATT 2571 0 2281 .......................................................................................... 2370 T T C N G G D G T D W N V I Q N W S G T Y G G D V N K Y G R Query_1 2572 ACTACGTGTAACGGTGGTGATGGGACAGATTGGAATGTTATTCAGAACTGGAGTGGAACCTATGGTGGTGATGTGAATAAGTATGGCCGT 2661 0 2371 .......................................................................................... 2460 E L S Q K N Q L L N G Q Y G A W R S I G L H T E P A A F D V Query_1 2662 GAATTGTCACAGAAGAATCAGTTGTTGAACGGTCAGTATGGGGCATGGCGCAGCATCGGATTGCATACGGAACCGGCAGCTTTTGATGTG 2751 0 2461 .................................G.A...................................................... 2550 N G V W S E E R M C Q L M E T K I R L A E Q A K D S V C G Q Query_1 2752 AATGGTGTATGGAGCGAGGAGCGTATGTGCCAGCTGATGGAAACCAAGATACGACTGGCGGAACAGGCAAAGGACAGTGTGTGCGGACAA 2841 0 2551 .......................................................................................... 2640 F Q W I F S S H D N P G R R Q P D E A Y R R I D K V G P F N Query_1 2842 TTCCAATGGATATTCAGCAGTCATGATAATCCGGGACGCCGCCAGCCGGATGAAGCGTATCGCCGGATAGATAAGGTGGGACCATTTAAT 2931 0 2641 .......................................................................................... 2730 Y K G L V T P W E E P L D V Y Y M Y R A N Y V P A S E D P M Query_1 2932 TATAAAGGATTGGTGACTCCGTGGGAGGAGCCGCTGGATGTATATTATATGTATCGCGCGAACTATGTGCCGGCTTCCGAAGATCCGATG 3021 0 2731 .......................................................................................... 2820 V Y L A S H T W E D R F A T G R R R A T I E A Y S N C D S V Query_1 3022 GTTTATCTGGCATCTCATACATGGGAGGACCGTTTTGCGACAGGCCGCAGACGGGCTACCATTGAGGCGTACAGCAATTGTGACAGTGTG 3111 0 2821 .......................................................................................... 2910 L L Y N D A V D A E Y L G R K L N H G V G T H F M W E N R D Query_1 3112 TTGCTGTACAATGATGCGGTGGATGCTGAATACTTGGGACGGAAATTGAATCATGGGGTGGGGACTCATTTCATGTGGGAAAACCGCGAT 3201 0 2911 .......................................................................................... 3000 I R Y N V L R A V G Y F K G K P A A E D V L V L N G L E K A Query_1 3202 ATCCGTTACAATGTGCTTCGTGCTGTGGGGTACTTTAAAGGAAAACCGGCAGCGGAAGATGTGCTGGTGCTGAACGGTCTGGAAAAAGCT 3291 0 3001 .......................................................................................... 3090 P H F E A L Y R G S A I V P V A A D R L N G T D L L K G A Query_1 3292 CCTCATTTTGAAGCTCTTTACCGTGGTTCTGCTATAGTGCCTGTTGCTGCCGACCGGTTGAACGGAACTGATTTGTTGAAAGGAGCTGA- 3380 0 3091 .........................................................................................- 3179 1 1180 ........-.N......A 1164 E G Y T Y L Y R L N C G G D A Y T D T Y G Q V W A Q D N S R Query_1 3381 GGGATATACGTAT-CTTTATCGTTTGAATTGCGGAGGAGACGCTTATACGGATACGTA-TGGTCAGGTCTGGGCGCAGGATAACAGCCGT 3468 0 3180 .............-............................................-............................... 3267 1 1163 ..NNN........T.N....TCG...N-......N.-...NNGN...NN.-.......N....TCA..TNN.......A........... 1077 Y S H S W A E S F V H P S D S V Q L L S P Y Q A S Q R T T N Query_1 3469 TATTCTCATTCGTGGGCGGAATCGTTTGTTCATCCGTCGGATAGTGTACAGTTGTTGAGCCCTTATCAGGCGAGCCAGCGTACCACGAAT 3558 0 3268 .......................................................................................... 3357 1 1076 .........CG...............-....................N..........N.NT.AT................N........ 988 D P I H G T R D W E L F Q T F R F G R H K L N F R F P V P D Query_1 3559 GATCCCATTCATGGCACTAGGGACTGGGAACTGTTCCAGACTTTCCGCTTCGGACGCCATAAGCTGAACTTCCGCTTTCCCGTTCCGGAT 3648 0 3358 .......................................................................................... 3447 1 987 ...........................-.............................................................. 899 G E Y R V E L Y F T E P W H G T G G G V Q T D C E G L R I F Query_1 3649 GGCGAGTATCGTGTGGAACTTTATTTTACAGAACCCTGGCATGGTACAGGAGGCGGCGTACAGACGGATTGTGAAGGCTTGCGTATTTTT 3738 0 3448 .......................................................................................... 3537 1 898 .................-........................................................................ 810 D V A V N D K V L L D D L D V W A E A G H D G A C K K V V N Query_1 3739 GATGTAGCGGTAAATGATAAAGTACTTCTGGACGACTTGGATGTATGGGCTGAAGCGGGTCATGACGGCGCTTGCAAAAAGGTGGTGAAT 3828 0 3538 .......................................................................................... 3627 1 809 .......................................................................................... 720 A I V K G G V L K I N F P E V K A G Q A L I C G I A I A C K Query_1 3829 GCTATCGTGAAAGGGGGAGTCTTGAAGATTAATTTTCCGGAGGTGAAAGCGGGGCAAGCTTTGATTTGTGGCATTGCGATAGCTTGTAAA 3918 0 3628 .......................................................................................... 3717 1 719 .......................................................................................... 630 G D L D S V R S F S A H S F S W A A Q D K E V M E K T P K E Query_1 3919 GGCGACTTGGATTCCGTTCGCTCTTTTTCTGCCCATAGCTTCAGCTGGGCTGCACAAGACAAGGAGGTGATGGAAAAGACTCCCAAAGAA 4008 0 3718 .......................................................................................... 3807 1 629 .......................................................................................... 540 L L P E D K N A R A N V T Y Q A E D A V L K G K F I K K E V Query_1 4009 TTATTGCCTGAAGATAAAAACGCACGTGCCAATGTGACTTATCAAGCGGAGGATGCTGTGCTGAAAGGTAAGTTCATAAAGAAAGAGGTT 4098 0 3808 .......................................................................................... 3897 1 539 .......................................................................................... 450 K K Q T G V F F G K G T Q S S I T W N I S T G L A Q V Y A L Query_1 4099 AAGAAACAAACCGGGGTTTTCTTTGGTAAAGGTACACAAAGCAGTATTACTTGGAACATTTCTACCGGTCTGGCACAAGTCTATGCTCTT 4188 0 3898 .......................................................................................... 3987 1 449 .......................................................................................... 360 R F K Y M N V T G K P M K V R M Q F I D S K G V V L K E D N Query_1 4189 CGTTTTAAATACATGAATGTTACAGGGAAGCCTATGAAGGTGCGTATGCAGTTCATTGATTCCAAAGGGGTGGTACTGAAGGAAGATAAT 4278 0 3988 .......................................................................................... 4077 1 359 .......................................................................................... 270 L T F A E T P G K W R M L S T T T G T Y I N A G Y Y K V V L Query_1 4279 CTGACTTTTGCCGAAACTCCCGGAAAGTGGCGTATGCTGAGTACAACGACGGGTACGTATATCAATGCAGGCTATTATAAGGTGGTGCTT 4368 0 4078 .......................................................................................... 4167 0 290 ......G.. 298 2 443 ......G.. 451 1 269 .......................................................................................... 180 S A P D M E G L A L D A L D V Q * H W K W I T D P N S S Query_1 4369 TCC--GCA---CCGGATATGGAGGGACTGGCATTGGATGCGCTGGATGTGCAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCT 4453 0 4168 ...--...---.......................................... 4215 0 299 ...CT...TTG............ 321 2 452 ...CT...TTG............ 474 1 179 ...--...---............................................................................... 95 S V D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 4454 CCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGC 4535 1 94 .............................................................................N.G.. 13 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 30580290 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 6,699 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200065.109 (CMR200065.25-679.P(679).E499Q.pMCSG68)
EDKLINQDWSFRFSHQVNANAARRVDLPHTWNAQDALGGKHDYKRGIGNYTKKIFIRPEWQSKRLFLRFEGANCVSNVFVNGKHIGEHRGGYGAFVFEITDKVEYGKENTLLVRVNNGEQLDVMPLVGDFNFYGGIYRDVHLLLTDNLCISPLDYASSGFYLIQQQITDKQAAICARINLSNGTGELRKAVLRLQVNDGKKTVYETEKEVSMIPHTDVQVENIEFILKNPRLWNGTQDPFMYQTVVTLIKDGKELDKVEQPLGVRYYITDPDKGFFLNGKHLPLHGVCRHQERAEVGNALYPVHHEEDTRIMLDMGVNAVRLAHYPQATYMYDLMDKYGIVTWAEIPFVGPGGYADKGFVDQPSFRENGKEQLKEMIRQHYNHPSICFWGLFNELKEQGDNPVEYIKELNAMAHREDPTRPTTSASNQDGALNFITDHIAWNRYDGWYGATPATLATWLDATHKNHPEIKIAISEYGAGASIYHQQDSLVQTVPGSWWHPENWQTEYHIQNWKIINERPYVWASFVWNMFDFGAAHRMEGDRSGINDKGLVTHDRKIKKDAYYFYRANWNPEPMIYIAGRRNVNRVKPLVDVQVFSNVEEVILIVNDCQCRRMKPDSLKVCLFKEVPLRKGRNEIEVRASDSKKQLIDRCTWILQ
GAAGATAAGCTTATCAATCAGGATTGGAGTTTCCGTTTTTCCCATCAAGTAAATGCTAATGCTGCACGTAGGGTAGACTTGCCTCATACTTGGAATGCACAGGACGCTTTGGGTGGTAAGCACGATTACAAGAGGGGAATAGGTAATTATACTAAAAAAATATTCATCCGTCCCGAGTGGCAAAGTAAGCGGCTTTTTTTACGTTTTGAGGGAGCGAATTGCGTGAGCAATGTCTTTGTAAATGGTAAACATATAGGTGAGCATCGGGGCGGTTATGGAGCTTTTGTCTTCGAGATAACCGATAAAGTGGAATATGGAAAAGAGAATACATTGCTGGTACGTGTCAATAATGGCGAGCAGTTGGATGTAATGCCATTGGTCGGTGATTTTAATTTTTATGGTGGCATTTATCGGGATGTACATCTGTTGCTGACTGATAACCTGTGTATCTCTCCGTTAGATTATGCATCGTCTGGGTTTTATCTCATACAGCAACAGATTACGGACAAACAGGCAGCTATTTGTGCACGCATCAATCTTTCTAATGGAACGGGAGAACTCCGAAAAGCAGTGCTCCGGTTGCAGGTTAATGATGGAAAAAAGACTGTGTATGAAACGGAGAAGGAAGTAAGTATGATTCCTCATACAGATGTACAGGTAGAGAATATAGAGTTCATACTGAAGAATCCGCGTCTTTGGAACGGGACGCAGGATCCATTCATGTATCAGACGGTGGTGACCTTGATTAAAGATGGAAAAGAGTTGGATAAAGTGGAACAGCCGTTAGGGGTACGTTATTACATCACGGATCCCGATAAAGGTTTTTTCTTGAATGGAAAACATTTGCCGCTGCATGGTGTATGCCGTCATCAGGAGAGGGCGGAAGTGGGTAATGCTCTTTATCCGGTGCATCACGAGGAAGATACTCGTATCATGCTTGATATGGGGGTGAATGCCGTTCGTCTGGCACATTATCCACAAGCTACCTATATGTATGATTTAATGGATAAATATGGCATTGTCACTTGGGCTGAGATTCCATTTGTGGGGCCGGGAGGATACGCTGATAAAGGTTTTGTTGATCAGCCTTCTTTCCGCGAAAATGGTAAGGAGCAGTTGAAAGAGATGATCCGCCAGCATTACAATCATCCTAGTATCTGTTTTTGGGGATTGTTCAATGAATTGAAAGAGCAGGGAGACAATCCTGTAGAGTATATAAAGGAGTTGAATGCTATGGCACATCGGGAGGATCCTACTCGTCCTACTACTTCTGCAAGTAATCAGGATGGCGCGTTGAATTTCATTACTGATCATATAGCTTGGAATCGCTATGATGGATGGTATGGGGCCACACCGGCTACGCTTGCTACATGGTTGGATGCTACTCATAAGAACCATCCCGAAATAAAAATTGCCATCAGCGAATATGGTGCTGGTGCTAGTATCTATCATCAGCAGGACTCATTGGTACAAACTGTTCCCGGAAGTTGGTGGCATCCAGAGAATTGGCAAACAGAATATCATATTCAGAATTGGAAAATAATAAATGAACGTCCGTATGTGTGGGCTAGTTTTGTGTGGAATATGTTTGATTTCGGTGCAGCTCATCGTATGGAGGGGGATCGTTCGGGCATCAATGACAAAGGACTGGTAACTCATGATCGTAAGATTAAAAAGGATGCTTATTATTTTTACCGGGCCAATTGGAATCCAGAACCTATGATTTATATCGCAGGGCGTCGTAATGTGAATCGGGTGAAGCCGCTCGTTGATGTACAGGTCTTTTCGAATGTAGAAGAAGTGATATTGATAGTGAATGACTGTCAGTGTAGAAGGATGAAGCCGGACAGTTTGAAAGTTTGTTTGTTCAAGGAGGTCCCTCTCAGAAAAGGAAGGAATGAAATAGAAGTACGTGCCAGTGATAGTAAAAAACAATTGATAGATCGATGCACTTGGATATTACAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,480 total letters Query= CMR200065.109 Length=2370 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3535 0.0 2_06G_G6_CMR200065.109_T7S.seq 253 4e-70 1_03G_G6_CMR200065.109_T7term.seq 214 4e-58 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 58 TTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGG 147 2 6 .....T....N............................................................................... 95 S H P Q F E K G T E N L Y F Q S N A E D K L I N Q D W S F Query_1 148 TCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGAA-GATAAGCTTATCAATCAGGATTGGAGTTTCCG 236 0 1 ...-................................ 35 0 28 ......C.T..TT............ 4 2 96 ......................................................C.. 152 2 1134 ........ 1127 R F S H Q V N A N A A R R V D L P H T W N A Q D A L G G K H Query_1 237 TTTTTCCCATCAAGTAAATGCTAATGCTGCACGTAGGGTAGACTTGCCTCATACTTGGAATGCACAGGACGCTTTGGGTGGTAAGCACGA 326 0 36 .......................................................................................... 125 2 1126 ... 1124 D Y K R G I G N Y T K K I F I R P E W Q S K R L F L R F E G Query_1 327 TTACAAGAGGGGAATAGGTAATTATACTaaaaaaaTATTCATCCGTCCCGAGTGGCAAAGTAAGCGGCtttttttACGTTTTGAGGGAGC 416 0 126 .......................................................................................... 215 A N C V S N V F V N G K H I G E H R G G Y G A F V F E I T D Query_1 417 GAATTGCGTGAGCAATGTCTTTGTAAATGGTAAACATATAGGTGAGCATCGGGGCGGTTATGGAGCTTTTGTCTTCGAGATAACCGATAA 506 0 216 .......................................................................................... 305 0 766 ..... 770 K V E Y G K E N T L L V R V N N G E Q L D V M P L V G D F N Query_1 507 AGTGGAATATGGAAAAGAGAATACATTGCTGGTACGTGTCAATAATGGCGAGCAGTTGGATGTAATGCCATTGGTCGGTGATTTTAATTT 596 0 306 .......................................................................................... 395 0 749 ..G........... 762 0 771 ....... 777 F Y G G I Y R D V H L L L T D N L C I S P L D Y A S S G F Y Query_1 597 TTATGGTGGCATTTATCGGGATGTACATCTGTTGCTGACTGATAACCTGTGTATCTCTCCGTTAGATTATGCATCGTCTGGGTTTTATCT 686 0 396 .......................................................................................... 485 0 500 ........... 490 0 656 ........... 646 0 819 ........... 809 L I Q Q Q I T D K Q A A I C A R I N L S N G T G E L R K A V Query_1 687 CATACAGCAACAGATTACGGACAAACAGGCAGCTATTTGTGCACGCATCAATCTTTCTAATGGAACGGGAGAACTCCGAAAAGCAGTGCT 776 0 486 .......................................................................................... 575 0 432 ........... 422 1 905 ..G 907 L R L Q V N D G K K T V Y E T E K E V S M I P H T D V Q V E Query_1 777 CCGGTTGCAGGTTAATGATGGAAAAAAGACTGTGTATGAAACGGAGAAGGAAGTAAGTATGATTCCTCATACAGATGTACAGGTAGAGAA 866 0 576 .......................................................................................... 665 0 427 ........... 417 0 1771 ........... 1781 1 908 ........... 918 N I E F I L K N P R L W N G T Q D P F M Y Q T V V T L I K D Query_1 867 TATAGAGTTCATACTGAAGAATCCGCGTCTTTGGAACGGGACGCAGGATCCATTCATGTATCAGACGGTGGTGACCTTGATTAAAGATGG 956 0 666 .......................................................................................... 755 0 311 ..T.... 317 G K E L D K V E Q P L G V R Y Y I T D P D K G F F L N G K H Query_1 957 AAAAGAGTTGGATAAAGTGGAACAGCCGTTAGGGGTACGTTATTACATCACGGATCCCGATAAAGGTTTTTTCTTGAATGGAAAACATTT 1046 0 756 .......................................................................................... 845 0 318 ....... 324 0 301 ............ 312 0 1066 ............ 1077 0 417 ........... 407 L P L H G V C R H Q E R A E V G N A L Y P V H H E E D T R I Query_1 1047 GCCGCTGCATGGTGTATGCCGTCATCAGGAGAGGGCGGAAGTGGGTAATGCTCTTTATCCGGTGCATCACGAGGAAGATACTCGTATCAT 1136 0 846 .......................................................................................... 935 M L D M G V N A V R L A H Y P Q A T Y M Y D L M D K Y G I V Query_1 1137 GCTTGATATGGGGGTGAATGCCGTTCGTCTGGCACATTATCCACAAGCTACCTATATGTATGATTTAATGGATAAATATGGCATTGTCAC 1226 0 936 .......................................................................................... 1025 T W A E I P F V G P G G Y A D K G F V D Q P S F R E N G K E Query_1 1227 TTGGGCTGAGATTCCATTTGTGGGGCCGGGAGGATACGCTGATAAAGGTTTTGTTGATCAGCCTTCTTTCCGCGAAAATGGTAAGGAGCA 1316 0 1026 .......................................................................................... 1115 0 814 ............ 825 Q L K E M I R Q H Y N H P S I C F W G L F N E L K E Q G D N Query_1 1317 GTTGAAAGAGATGATCCGCCAGCATTACAATCATCCTAGTATCTGTTTTTGGGGATTGTTCAATGAATTGAAAGAGCAGGGAGACAATCC 1406 0 1116 .......................................................................................... 1205 P V E Y I K E L N A M A H R E D P T R P T T S A S N Q D G A Query_1 1407 TGTAGAGTATATAAAGGAGTTGAATGCTATGGCACATCGGGAGGATCCTACTCGTCCTACTACTTCTGCAAGTAATCAGGATGGCGCGTT 1496 0 1206 .......................................................................................... 1295 L N F I T D H I A W N R Y D G W Y G A T P A T L A T W L D A Query_1 1497 GAATTTCATTACTGATCATATAGCTTGGAATCGCTATGATGGATGGTATGGGGCCACACCGGCTACGCTTGCTACATGGTTGGATGCTAC 1586 0 1296 .......................................................................................... 1385 T H K N H P E I K I A I S Q Y G A G A S I Y H Q Q D S L V Q Query_1 1587 TCATAAGAACCATCCCGAAATAAAAATTGCCATCAGCCAGTATGGTGCTGGTGCTAGTATCTATCATCAGCAGGACTCATTGGTACAAAC 1676 0 1386 .....................................G.A.................................................. 1475 2 457 ...... 462 T V P G S W W H P E N W Q T E Y H I Q N W K I I N E R P Y V Query_1 1677 TGTTCCCGGAAGTTGGTGGCATCCAGAGAATTGGCAAACAGAATATCATATTCAGAATTGGAAAATAATAAATGAACGTCCGTATGTGTG 1766 0 1476 .......................................................................................... 1565 2 463 ...... 468 W A S F V W N M F D F G A A H R M E G D R S G I N D K G L V Query_1 1767 GGCTAGTTTTGTGTGGAATATGTTTGATTTCGGTGCAGCTCATCGTATGGAGGGGGATCGTTCGGGCATCAATGACAAAGGACTGGTAAC 1856 0 1566 .......................................................................................... 1655 T H D R K I K K D A Y Y F Y R A N W N P E P M I Y I A G R R Query_1 1857 TCATGATCGTAAGATTAAAAAGGATGCTTATTATTTTTACCGGGCCAATTGGAATCCAGAACCTATGATTTATATCGCAGGGCGTCGTAA 1946 0 1656 .......................................................................................... 1745 N V N R V K P L V D V Q V F S N V E E V I L I V N D C Q C R Query_1 1947 TGTGAATCGGGTGAAGCCGCTCGTTGATGTACAGGTCTTTTCGAATGTAGAAGAAGTGATATTGATAGTGAATGACTGTCAGTGTAGAAG 2036 0 1746 .......................................................................................... 1835 0 649 ........... 659 R M K P D S L K V C L F K E V P L R K G R N E I E V R A S D Query_1 2037 GATGAAGCCGGACAGTTTGAAAGTTTGTTTGTTCAAGGAGGTCCCTCTCAGAAAAGGAAGGAATGAAATAGAAGTACGTGCCAGTGATAG 2126 0 1836 .......................................................................................... 1925 S K K Q L I D R C T W I L Q * H W K W I T D P N S S S V D K Query_1 2127 TAAAAAACAATTGATAGATCGATGCACTTGGATATTACAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCT 2216 0 1926 ........................................ 1965 1 139 ....A.................................................... 83 L A A A L E H H H H H H * D P A A N K A R K Query_1 2217 TGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAG 2280 1 82 ................................................................ 19 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 10421437 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,480 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200069.106 (CMR200069.20-1019.P(1019).E478A.pMCSG68)
QQQPEWQSQYAVGLNKLAPHTYVWPYQTISDIRNGDYESSPYYMSLNGSWKFHWVKNPDNRPKDFYKPEFYTGGWADIQVPGNWERQGYGTAIYVNETYEFDDKLFHFKKNPPLVPYKENEVGSYRRTFTIPADWEDRRVVICCEGVISFYYIWVNGHLLGYNQGSKTPAEWDITDKLKKGENTVALEVYRWSAGSYLECQDMWRLSGIERDVYLYSTPKQYIADYKVTSSLDKQQYKEGIFGLEATIEGPSTSMTTFAYRLEDKTGKAVLQGEYPIKSKGLSHFLKLDEQSLPDVEVWSAEHPNLYTLILELKDVTGKVTELTGCEVGFRTSEIKDGRFCINGVPVLVKGTNRHEHSQLGRTVSKELMEKDIKLMKQHNINTVRNSHYPTHPYWYQLCDRYGLYMIDEANIESHGMGYGPASLAKDSTWLPAHMDRTQRMYERSKNHPAIVIWSLGNEAGNGINFERTYDWMKSVEKSRPVQYERAEQNYNTDIYCRMYRSVEELMAYARQTEPKVYRPFIMTEYLHTMGNSGGGLKEYMHVFETEPIVQGGCIWDWVDQSFREIDKDGKWYWSYGGDYGPKDVPSFGNFCCNGLVNAVREPHPHLIEVKKEYQYIKSVLTDPKKLTVEVKNWYDFTNLNAYTLHWQVMGDDGKVIAEGTRKADCAPHEAVTFSLGAVKLPSTIREAYLNLSWTPDKATPFIGTHDEVAYDQFVLSANKGYRAPEIKLADKVKIDIDPATGALRSYIYKGKEMLSSPVVLSLYRPVTDNDSREKTGGAKVWRKEGLDNMIQKATFVKASETGGKAEVELWNAKGVKLGMATFVYTLQSNGALKVKTTFLPDTSAVTSLARVGLAFEMPYAYNKVSYLGRGEHETYIDRNQSGCIGIYHTDAERMFHYYVKPQATGNRTDVRWMELADEAGEGLSFRSDKVFQFCVIPFTDSNVDKATHINKLERTGIINVHLDAEQSGVGTATCGPGVLPPYRVPVEKHTFEFMICPLK
CAACAACAACCCGAATGGCAGAGCCAATATGCTGTCGGACTGAACAAACTGGCCCCTCATACTTATGTGTGGCCTTATCAAACCATATCTGATATCCGTAACGGAGATTATGAATCTTCTCCTTATTATATGAGTCTGAACGGTAGTTGGAAATTCCATTGGGTAAAGAATCCCGATAACCGTCCGAAGGATTTTTATAAACCGGAGTTTTATACAGGAGGATGGGCGGATATTCAGGTTCCCGGCAATTGGGAACGCCAAGGATATGGAACAGCTATTTATGTAAACGAAACCTATGAGTTCGATGACAAACTGTTCCATTTTAAAAAGAATCCGCCTTTAGTTCCATATAAAGAGAATGAAGTCGGTTCCTACAGACGTACCTTTACAATTCCTGCCGATTGGGAAGACCGCCGTGTGGTTATCTGTTGTGAAGGAGTGATTTCTTTTTACTATATTTGGGTGAACGGACATCTGTTGGGCTATAATCAAGGTTCAAAGACACCGGCCGAATGGGATATCACTGATAAATTGAAGAAAGGTGAGAATACGGTGGCATTGGAGGTTTACCGTTGGAGCGCCGGATCGTATTTGGAGTGCCAGGATATGTGGCGGTTGAGTGGTATTGAACGTGATGTGTATCTTTACAGCACTCCTAAACAGTATATAGCCGATTATAAAGTAACCTCTTCTTTAGACAAACAACAATATAAAGAAGGTATTTTCGGTTTGGAAGCTACCATTGAAGGGCCTTCTACTTCCATGACTACCTTTGCTTACCGACTGGAAGATAAAACCGGTAAGGCGGTTCTGCAAGGTGAATATCCTATCAAATCGAAGGGATTGAGCCATTTCTTGAAGTTGGATGAACAAAGTTTGCCGGATGTGGAAGTCTGGAGTGCTGAGCATCCGAATCTTTATACACTGATCTTGGAATTGAAAGATGTAACAGGTAAAGTAACGGAACTTACCGGATGTGAAGTAGGTTTCCGCACTTCGGAAATTAAGGACGGACGTTTTTGCATCAATGGTGTGCCGGTTTTGGTAAAGGGAACGAACCGTCATGAACATTCCCAATTAGGACGGACTGTAAGCAAGGAACTGATGGAAAAAGATATCAAATTGATGAAACAGCATAATATCAATACGGTCCGCAATTCGCACTACCCTACCCATCCTTATTGGTATCAGCTTTGTGATCGTTATGGACTTTATATGATAGATGAAGCTAATATTGAATCGCATGGCATGGGGTATGGACCTGCTTCATTGGCCAAAGATTCCACTTGGTTGCCTGCCCACATGGATCGTACCCAACGTATGTATGAGCGTTCCAAGAATCATCCGGCTATCGTTATCTGGTCATTGGGGAATGAAGCCGGAAACGGTATTAACTTCGAACGTACATATGACTGGATGAAGTCAGTGGAGAAAAGTCGTCCTGTACAATATGAACGGGCCGAACAGAATTATAATACCGATATCTATTGCCGTATGTATCGCAGCGTGGAAGAACTCATGGCCTATGCCCGCCAGACAGAACCTAAAGTTTATCGTCCTTTCATCATGACAGAGTATCTGCATACTATGGGTAATAGTGGCGGAGGATTAAAGGAATATATGCATGTATTCGAAACCGAACCGATTGTTCAGGGAGGTTGTATCTGGGATTGGGTGGATCAATCTTTCCGCGAGATAGATAAGGATGGTAAATGGTATTGGAGTTATGGTGGGGATTATGGTCCGAAGGATGTGCCCAGTTTTGGCAATTTCTGTTGTAACGGGTTGGTCAATGCAGTCCGCGAACCGCATCCGCACCTCATTGAAGTGAAGAAGGAGTATCAATATATCAAATCGGTACTGACCGATCCTAAGAAGCTGACAGTGGAAGTCAAGAACTGGTATGACTTTACCAATTTGAATGCCTATACGTTGCACTGGCAGGTCATGGGGGATGATGGGAAAGTGATAGCAGAAGGAACTCGAAAAGCAGACTGTGCCCCTCATGAGGCTGTAACATTCTCTTTGGGTGCTGTAAAGTTGCCTTCTACGATTCGTGAGGCTTATTTGAATCTGTCTTGGACGCCTGATAAGGCAACTCCCTTTATTGGAACTCATGACGAAGTGGCCTATGACCAGTTTGTACTGTCTGCCAATAAAGGGTATCGTGCGCCGGAAATCAAATTGGCTGATAAGGTGAAGATAGATATTGATCCTGCAACCGGCGCTTTACGCTCTTATATATATAAAGGTAAAGAGATGCTTTCATCTCCTGTAGTTCTCAGCCTTTATCGTCCTGTTACGGACAATGACAGCAGGGAAAAAACAGGAGGAGCCAAAGTTTGGAGGAAAGAAGGTTTGGATAATATGATTCAAAAAGCTACTTTTGTCAAAGCATCTGAAACAGGTGGTAAAGCGGAAGTAGAGTTATGGAATGCTAAAGGCGTAAAGTTGGGAATGGCTACCTTTGTATATACCTTGCAGAGCAATGGTGCATTAAAAGTAAAGACAACTTTCTTGCCGGATACTTCTGCTGTTACTTCATTGGCTAGAGTGGGCCTGGCATTTGAAATGCCGTATGCCTATAACAAGGTTTCTTATTTGGGACGTGGTGAACATGAAACTTATATAGACCGTAATCAGTCTGGATGTATCGGAATTTACCATACAGATGCAGAACGTATGTTCCATTATTATGTGAAGCCCCAGGCTACAGGAAATCGTACGGATGTGCGTTGGATGGAATTGGCGGATGAGGCAGGAGAAGGTTTGTCTTTCCGTTCGGATAAAGTGTTTCAGTTCTGTGTTATTCCATTTACGGATTCTAATGTAGATAAAGCTACTCATATTAATAAACTTGAGAGAACAGGGATTATAAATGTTCACTTAGATGCGGAACAGTCGGGGGTAGGTACTGCGACTTGTGGTCCGGGGGTATTGCCGCCATATCGTGTTCCGGTGGAGAAGCATACGTTTGAGTTTATGATTTGTCCATTAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,740 total letters Query= CMR200069.106 Length=3405 Score E Sequences producing significant alignments: (Bits) Value 0_wt 5402 0.0 1_03H_H6_CMR200069.106_T7term.seq 1826 0.0 2_06H_H6_CMR200069.106_T7S.seq 877 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 58 TTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGG 147 2 3 .....G...TNN..............-.......T.T............................AA...............T....... 91 S H P Q F E K G T E N L Y F Q S N A Q Q Q P E W Q S Q Y A V Query_1 148 TCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAACAACAACCCGAATGGCAGAGCCAATATGCTGTC 237 0 1 .................................... 36 2 92 .......................................................................................... 181 G L N K L A P H T Y V W P Y Q T I S D I R N G D Y E S S P Y Query_1 238 GGACTGAACAAACTGGCCCCTCATACTTATGTGTGGCCTTATCAAACCATATCTGATATCCGTAACGGAGATTATGAATCTTCTCCTTAT 327 0 37 .......................................................................................... 126 1 993 ........... 1003 2 182 .......................................................................................... 271 Y M S L N G S W K F H W V K N P D N R P K D F Y K P E F Y T Query_1 328 TATATGAGTCTGAACGGTAGTTGGAAATTCCATTGGGTAAAGAATCCCGATAACCGTCCGAAGGATTTTTATAAACCGGAGTTTTATACA 417 0 127 .......................................................................................... 216 0 1742 ........... 1752 2 272 ...............................................................................G.......... 361 G G W A D I Q V P G N W E R Q G Y G T A I Y V N E T Y E F D Query_1 418 GGAGGATGGGCGGATATTCAGGTTCCCGGCAATTGGGAACGCCAAGGATATGGAACAGCTATTTATGTAAACGAAACCTATGAGTTCGAT 507 0 217 .......................................................................................... 306 2 362 ..............................N....................................N...................... 451 D K L F H F K K N P P L V P Y K E N E V G S Y R R T F T I P Query_1 508 GACAAACTGTTCCATTTTAAAAAGAATCCGCCTTTAGTTCCATATAAAGAGAATGAAGTCGGTTCCTACAGACGTACCTTTACAATTCCT 597 0 307 .......................................................................................... 396 2 452 .............N.......................................N................... 524 A D W E D R R V V I C C E G V I S F Y Y I W V N G H L L G Y Query_1 598 GCCGATTGGGAAGACCGCCGTGTGGTTATCTGTTGTGAAGGAGTGATTTCTTTTTACTATATTTGGGTGAACGGACATCTGTTGGGCTAT 687 0 397 .......................................................................................... 486 N Q G S K T P A E W D I T D K L K K G E N T V A L E V Y R W Query_1 688 AATCAAGGTTCAAAGACACCGGCCGAATGGGATATCACTGATAAATTGAAGAAAGGTGAGAATACGGTGGCATTGGAGGTTTACCGTTGG 777 0 487 .......................................................................................... 576 S A G S Y L E C Q D M W R L S G I E R D V Y L Y S T P K Q Y Query_1 778 AGCGCCGGATCGTATTTGGAGTGCCAGGATATGTGGCGGTTGAGTGGTATTGAACGTGATGTGTATCTTTACAGCACTCCTAAACAGTAT 867 0 577 .......................................................................................... 666 0 2040 ........... 2030 I A D Y K V T S S L D K Q Q Y K E G I F G L E A T I E G P S Query_1 868 ATAGCCGATTATAAAGTAACCTCTTCTTTAGACAAACAACAATATAAAGAAGGTATTTTCGGTTTGGAAGCTACCATTGAAGGGCCTTCT 957 0 667 .......................................................................................... 756 T S M T T F A Y R L E D K T G K A V L Q G E Y P I K S K G L Query_1 958 ACTTCCATGACTACCTTTGCTTACCGACTGGAAGATAAAACCGGTAAGGCGGTTCTGCAAGGTGAATATCCTATCAAATCGAAGGGATTG 1047 0 757 .......................................................................................... 846 S H F L K L D E Q S L P D V E V W S A E H P N L Y T L I L E Query_1 1048 AGCCATTTCTTGAAGTTGGATGAACAAAGTTTGCCGGATGTGGAAGTCTGGAGTGCTGAGCATCCGAATCTTTATACACTGATCTTGGAA 1137 0 847 .......................................................................................... 936 L K D V T G K V T E L T G C E V G F R T S E I K D G R F C I Query_1 1138 TTGAAAGATGTAACAGGTAAAGTAACGGAACTTACCGGATGTGAAGTAGGTTTCCGCACTTCGGAAATTAAGGACGGACGTTTTTGCATC 1227 0 937 .......................................................................................... 1026 N G V P V L V K G T N R H E H S Q L G R T V S K E L M E K D Query_1 1228 AATGGTGTGCCGGTTTTGGTAAAGGGAACGAACCGTCATGAACATTCCCAATTAGGACGGACTGTAAGCAAGGAACTGATGGAAAAAGAT 1317 0 1027 .......................................................................................... 1116 I K L M K Q H N I N T V R N S H Y P T H P Y W Y Q L C D R Y Query_1 1318 ATCAAATTGATGAAACAGCATAATATCAATACGGTCCGCAATTCGCACTACCCTACCCATCCTTATTGGTATCAGCTTTGTGATCGTTAT 1407 0 1117 .......................................................................................... 1206 G L Y M I D E A N I E S H G M G Y G P A S L A K D S T W L P Query_1 1408 GGACTTTATATGATAGATGAAGCTAATATTGAATCGCATGGCATGGGGTATGGACCTGCTTCATTGGCCAAAGATTCCACTTGGTTGCCT 1497 0 1207 .......................................................................................... 1296 A H M D R T Q R M Y E R S K N H P A I V I W S L G N A A G N Query_1 1498 GCCCACATGGATCGTACCCAACGTATGTATGAGCGTTCCAAGAATCATCCGGCTATCGTTATCTGGTCATTGGGGAATGCTGCCGGAAAC 1587 0 1297 ...............................................................................AA......... 1386 G I N F E R T Y D W M K S V E K S R P V Q Y E R A E Q N Y N Query_1 1588 GGTATTAACTTCGAACGTACATATGACTGGATGAAGTCAGTGGAGAAAAGTCGTCCTGTACAATATGAACGGGCCGAACAGAATTATAAT 1677 0 1387 .......................................................................................... 1476 T D I Y C R M Y R S V E E L M A Y A R Q T E P K V Y R P F I Query_1 1678 ACCGATATCTATTGCCGTATGTATCGCAGCGTGGAAGAACTCATGGCCTATGCCCGCCAGACAGAACCTAAAGTTTATCGTCCTTTCATC 1767 0 1477 .......................................................................................... 1566 0 2288 ........... 2298 1 848 ........... 838 M T E Y L H T M G N S G G G L K E Y M H V F E T E P I V Q G Query_1 1768 ATGACAGAGTATCTGCATACTATGGGTAATAGTGGCGGAGGATTAAAGGAATATATGCATGTATTCGAAACCGAACCGATTGTTCAGGGA 1857 0 1567 .......................................................................................... 1656 G C I W D W V D Q S F R E I D K D G K W Y W S Y G G D Y G P Query_1 1858 GGTTGTATCTGGGATTGGGTGGATCAATCTTTCCGCGAGATAGATAAGGATGGTAAATGGTATTGGAGTTATGGTGGGGATTATGGTCCG 1947 0 1657 .......................................................................................... 1746 0 182 ..... 186 2 327 ..... 331 K D V P S F G N F C C N G L V N A V R E P H P H L I E V K K Query_1 1948 AAGGATGTGCCCAGTTTTGGCAATTTCTGTTGTAACGGGTTGGTCAATGCAGTCCGCGAACCGCATCCGCACCTCATTGAAGTGAAGAAG 2037 0 1747 .......................................................................................... 1836 0 187 ...... 192 2 332 ...... 337 E Y Q Y I K S V L T D P K K L T V E V K N W Y D F T N L N A Query_1 2038 GAGTATCAATATATCAAATCGGTACTGACCGATCCTAAGAAGCTGACAGTGGAAGTCAAGAACTGGTATGACTTTACCAATTTGAATGCC 2127 0 1837 .......................................................................................... 1926 Y T L H W Q V M G D D G K V I A E G T R K A D C A P H E A Query_1 2128 TATACGTTGCACTGGCAGGTCATGGGGGATGATGGGAAAGTGATAGCAGAAGGAACTCGAAAAGCAGACTGTGCCCCTCATGAGGC--TG 2215 0 1927 ......................................................................................--.. 2014 1 1189 ......GA..NT......NNN-.N.GN.....C.NGA...G...N...NN-.........GAN.NG.. 1124 V T F S L G A V K L P S T I R E A Y L N L S W T P D K A T Query_1 2216 TAACATTCTCTTTGGGTGCTGTAAAGTTG--CCTTCTACGATTCGTGAGGCTTATTTG-AATCTGTCTTGGACGCCTGATAAGGCAACTC 2302 0 2015 .............................--...........................-............................... 2101 0 2163 . 2163 0 653 ........... 643 1 1123 ...........CN..N...N........TGC....NN...NNNNNN-.N...A.....G....NN...-...................N. 1036 1 975 . 975 P F I G T H D E V A Y D Q F V L S A N K G Y R A P E I K L A Query_1 2303 CCTTTATTGGAACTCATGACGAAGTGGCCTATGACCAGTTTGTACTGTCTGCCAATAAAGGGTATCGTGCGCCGGAAATCAAATTGGCTG 2392 0 2102 .......................................................................................... 2191 0 2162 ..........C.GA..GT..A..C...T.ATA.G...C..C..CA..AG.T........... 2101 1 1035 .............N.....N..........N..G............-........................................... 947 1 976 ..........C.GAAG.-..A..C.....ANA.G...C..CN.CA..NG.T........... 1036 D K V K I D I D P A T G A L R S Y I Y K G K E M L S S P V Query_1 2393 ATAAGGTGAAGATAGATATTGATCCTGCAACCGGCGCTTT-ACGCTCTTATA-TATATAA-AG-GTAAAGAGATGCTTTCATCTCCTGTA 2478 0 2192 ........................................-...........-.......-..-.......................... 2277 0 2257 ....-..-..-.....-.......G..C...... 2228 1 946 ......NN................N......N........T...........A.......-..-.........N................ 859 V L S L Y R P V T D N D S R E K T G G A K V W R K E G L D Query_1 2479 GTTCTCAGCCTTTATCGTCCTGTTACGGACAATGACAGCAGGGAAAAAACAGGAGGAGCCAAAGTTTGGAGGAAA-GAAGGTTTGGATAA 2567 0 2278 ...........................................................................-.............. 2366 0 1550 ........... 1560 1 858 ........................C..................................................A.............. 769 N M I Q K A T F V K A S E T G G K A E V E L W N A K G V K L Query_1 2568 TATGATTCAAAAAGCTACTTTTGTCAAAGCATCTGAAACAGGTGGTAAAGCGGAAGTAGAGTTATGGAATGCTAAAGGCGTAAAGTTGGG 2657 0 2367 .......................................................................................... 2456 1 768 .......................................................................................... 679 G M A T F V Y T L Q S N G A L K V K T T F L P D T S A V T S Query_1 2658 AATGGCTACCTTTGTATATACCTTGCAGAGCAATGGTGCATTAAAAGTAAAGACAACTTTCTTGCCGGATACTTCTGCTGTTACTTCATT 2747 0 2457 .......................................................................................... 2546 1 678 .......................................................................................... 589 L A R V G L A F E M P Y A Y N K V S Y L G R G E H E T Y I D Query_1 2748 GGCTAGAGTGGGCCTGGCATTTGAAATGCCGTATGCCTATAACAAGGTTTCTTATTTGGGACGTGGTGAACATGAAACTTATATAGACCG 2837 0 2547 .......................................................................................... 2636 1 588 .......................................................................................... 499 R N Q S G C I G I Y H T D A E R M F H Y Y V K P Q A T G N R Query_1 2838 TAATCAGTCTGGATGTATCGGAATTTACCATACAGATGCAGAACGTATGTTCCATTATTATGTGAAGCCCCAGGCTACAGGAAATCGTAC 2927 0 2637 .......................................................................................... 2726 1 498 .......................................................................................... 409 T D V R W M E L A D E A G E G L S F R S D K V F Q F C V I P Query_1 2928 GGATGTGCGTTGGATGGAATTGGCGGATGAGGCAGGAGAAGGTTTGTCTTTCCGTTCGGATAAAGTGTTTCAGTTCTGTGTTATTCCATT 3017 0 2727 .......................................................................................... 2816 1 408 .......................................................................................... 319 F T D S N V D K A T H I N K L E R T G I I N V H L D A E Q S Query_1 3018 TACGGATTCTAATGTAGATAAAGCTACTCATATTAATAAACTTGAGAGAACAGGGATTATAAATGTTCACTTAGATGCGGAACAGTCGGG 3107 0 2817 .......................................................................................... 2906 1 318 .......................................................................................... 229 G V G T A T C G P G V L P P Y R V P V E K H T F E F M I C P Query_1 3108 GGTAGGTACTGCGACTTGTGGTCCGGGGGTATTGCCGCCATATCGTGTTCCGGTGGAGAAGCATACGTTTGAGTTTATGATTTGTCCATT 3197 0 2907 .......................................................................................... 2996 1 228 .......................................................................................... 139 L K * H W K W I T D P N S S S V D K L A A A L E H H H H H H Query_1 3198 AAAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTG 3287 0 2997 .... 3000 1 138 .......................................................................................... 49 * D P A A N K A R Query_1 3288 AGATCCGGCTGCTAACAAAGCCCGAAA 3314 1 48 .......................N... 22 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 15871482 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,740 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200069.108 (CMR200069.20-1019.P(1019).E478Q.pMCSG68)
QQQPEWQSQYAVGLNKLAPHTYVWPYQTISDIRNGDYESSPYYMSLNGSWKFHWVKNPDNRPKDFYKPEFYTGGWADIQVPGNWERQGYGTAIYVNETYEFDDKLFHFKKNPPLVPYKENEVGSYRRTFTIPADWEDRRVVICCEGVISFYYIWVNGHLLGYNQGSKTPAEWDITDKLKKGENTVALEVYRWSAGSYLECQDMWRLSGIERDVYLYSTPKQYIADYKVTSSLDKQQYKEGIFGLEATIEGPSTSMTTFAYRLEDKTGKAVLQGEYPIKSKGLSHFLKLDEQSLPDVEVWSAEHPNLYTLILELKDVTGKVTELTGCEVGFRTSEIKDGRFCINGVPVLVKGTNRHEHSQLGRTVSKELMEKDIKLMKQHNINTVRNSHYPTHPYWYQLCDRYGLYMIDEANIESHGMGYGPASLAKDSTWLPAHMDRTQRMYERSKNHPAIVIWSLGNEAGNGINFERTYDWMKSVEKSRPVQYERAEQNYNTDIYCRMYRSVEELMAYARQTEPKVYRPFIMTEYLHTMGNSGGGLKEYMHVFETEPIVQGGCIWDWVDQSFREIDKDGKWYWSYGGDYGPKDVPSFGNFCCNGLVNAVREPHPHLIEVKKEYQYIKSVLTDPKKLTVEVKNWYDFTNLNAYTLHWQVMGDDGKVIAEGTRKADCAPHEAVTFSLGAVKLPSTIREAYLNLSWTPDKATPFIGTHDEVAYDQFVLSANKGYRAPEIKLADKVKIDIDPATGALRSYIYKGKEMLSSPVVLSLYRPVTDNDSREKTGGAKVWRKEGLDNMIQKATFVKASETGGKAEVELWNAKGVKLGMATFVYTLQSNGALKVKTTFLPDTSAVTSLARVGLAFEMPYAYNKVSYLGRGEHETYIDRNQSGCIGIYHTDAERMFHYYVKPQATGNRTDVRWMELADEAGEGLSFRSDKVFQFCVIPFTDSNVDKATHINKLERTGIINVHLDAEQSGVGTATCGPGVLPPYRVPVEKHTFEFMICPLK
CAACAACAACCCGAATGGCAGAGCCAATATGCTGTCGGACTGAACAAACTGGCCCCTCATACTTATGTGTGGCCTTATCAAACCATATCTGATATCCGTAACGGAGATTATGAATCTTCTCCTTATTATATGAGTCTGAACGGTAGTTGGAAATTCCATTGGGTAAAGAATCCCGATAACCGTCCGAAGGATTTTTATAAACCGGAGTTTTATACAGGAGGATGGGCGGATATTCAGGTTCCCGGCAATTGGGAACGCCAAGGATATGGAACAGCTATTTATGTAAACGAAACCTATGAGTTCGATGACAAACTGTTCCATTTTAAAAAGAATCCGCCTTTAGTTCCATATAAAGAGAATGAAGTCGGTTCCTACAGACGTACCTTTACAATTCCTGCCGATTGGGAAGACCGCCGTGTGGTTATCTGTTGTGAAGGAGTGATTTCTTTTTACTATATTTGGGTGAACGGACATCTGTTGGGCTATAATCAAGGTTCAAAGACACCGGCCGAATGGGATATCACTGATAAATTGAAGAAAGGTGAGAATACGGTGGCATTGGAGGTTTACCGTTGGAGCGCCGGATCGTATTTGGAGTGCCAGGATATGTGGCGGTTGAGTGGTATTGAACGTGATGTGTATCTTTACAGCACTCCTAAACAGTATATAGCCGATTATAAAGTAACCTCTTCTTTAGACAAACAACAATATAAAGAAGGTATTTTCGGTTTGGAAGCTACCATTGAAGGGCCTTCTACTTCCATGACTACCTTTGCTTACCGACTGGAAGATAAAACCGGTAAGGCGGTTCTGCAAGGTGAATATCCTATCAAATCGAAGGGATTGAGCCATTTCTTGAAGTTGGATGAACAAAGTTTGCCGGATGTGGAAGTCTGGAGTGCTGAGCATCCGAATCTTTATACACTGATCTTGGAATTGAAAGATGTAACAGGTAAAGTAACGGAACTTACCGGATGTGAAGTAGGTTTCCGCACTTCGGAAATTAAGGACGGACGTTTTTGCATCAATGGTGTGCCGGTTTTGGTAAAGGGAACGAACCGTCATGAACATTCCCAATTAGGACGGACTGTAAGCAAGGAACTGATGGAAAAAGATATCAAATTGATGAAACAGCATAATATCAATACGGTCCGCAATTCGCACTACCCTACCCATCCTTATTGGTATCAGCTTTGTGATCGTTATGGACTTTATATGATAGATGAAGCTAATATTGAATCGCATGGCATGGGGTATGGACCTGCTTCATTGGCCAAAGATTCCACTTGGTTGCCTGCCCACATGGATCGTACCCAACGTATGTATGAGCGTTCCAAGAATCATCCGGCTATCGTTATCTGGTCATTGGGGAATGAAGCCGGAAACGGTATTAACTTCGAACGTACATATGACTGGATGAAGTCAGTGGAGAAAAGTCGTCCTGTACAATATGAACGGGCCGAACAGAATTATAATACCGATATCTATTGCCGTATGTATCGCAGCGTGGAAGAACTCATGGCCTATGCCCGCCAGACAGAACCTAAAGTTTATCGTCCTTTCATCATGACAGAGTATCTGCATACTATGGGTAATAGTGGCGGAGGATTAAAGGAATATATGCATGTATTCGAAACCGAACCGATTGTTCAGGGAGGTTGTATCTGGGATTGGGTGGATCAATCTTTCCGCGAGATAGATAAGGATGGTAAATGGTATTGGAGTTATGGTGGGGATTATGGTCCGAAGGATGTGCCCAGTTTTGGCAATTTCTGTTGTAACGGGTTGGTCAATGCAGTCCGCGAACCGCATCCGCACCTCATTGAAGTGAAGAAGGAGTATCAATATATCAAATCGGTACTGACCGATCCTAAGAAGCTGACAGTGGAAGTCAAGAACTGGTATGACTTTACCAATTTGAATGCCTATACGTTGCACTGGCAGGTCATGGGGGATGATGGGAAAGTGATAGCAGAAGGAACTCGAAAAGCAGACTGTGCCCCTCATGAGGCTGTAACATTCTCTTTGGGTGCTGTAAAGTTGCCTTCTACGATTCGTGAGGCTTATTTGAATCTGTCTTGGACGCCTGATAAGGCAACTCCCTTTATTGGAACTCATGACGAAGTGGCCTATGACCAGTTTGTACTGTCTGCCAATAAAGGGTATCGTGCGCCGGAAATCAAATTGGCTGATAAGGTGAAGATAGATATTGATCCTGCAACCGGCGCTTTACGCTCTTATATATATAAAGGTAAAGAGATGCTTTCATCTCCTGTAGTTCTCAGCCTTTATCGTCCTGTTACGGACAATGACAGCAGGGAAAAAACAGGAGGAGCCAAAGTTTGGAGGAAAGAAGGTTTGGATAATATGATTCAAAAAGCTACTTTTGTCAAAGCATCTGAAACAGGTGGTAAAGCGGAAGTAGAGTTATGGAATGCTAAAGGCGTAAAGTTGGGAATGGCTACCTTTGTATATACCTTGCAGAGCAATGGTGCATTAAAAGTAAAGACAACTTTCTTGCCGGATACTTCTGCTGTTACTTCATTGGCTAGAGTGGGCCTGGCATTTGAAATGCCGTATGCCTATAACAAGGTTTCTTATTTGGGACGTGGTGAACATGAAACTTATATAGACCGTAATCAGTCTGGATGTATCGGAATTTACCATACAGATGCAGAACGTATGTTCCATTATTATGTGAAGCCCCAGGCTACAGGAAATCGTACGGATGTGCGTTGGATGGAATTGGCGGATGAGGCAGGAGAAGGTTTGTCTTTCCGTTCGGATAAAGTGTTTCAGTTCTGTGTTATTCCATTTACGGATTCTAATGTAGATAAAGCTACTCATATTAATAAACTTGAGAGAACAGGGATTATAAATGTTCACTTAGATGCGGAACAGTCGGGGGTAGGTACTGCGACTTGTGGTCCGGGGGTATTGCCGCCATATCGTGTTCCGGTGGAGAAGCATACGTTTGAGTTTATGATTTGTCCATTAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,173 total letters Query= CMR200069.108 Length=3405 Score E Sequences producing significant alignments: (Bits) Value 0_wt 5402 0.0 1_04A_A7_CMR200069.108_T7term.seq 1700 0.0 Q Q Q P E W Q S Q Y A V G L N K L A P H T Y V W P Y Q T I S Query_1 202 CAACAACAACCCGAATGGCAGAGCCAATATGCTGTCGGACTGAACAAACTGGCCCCTCATACTTATGTGTGGCCTTATCAAACCATATCT 291 0 1 .......................................................................................... 90 D I R N G D Y E S S P Y Y M S L N G S W K F H W V K N P D N Query_1 292 GATATCCGTAACGGAGATTATGAATCTTCTCCTTATTATATGAGTCTGAACGGTAGTTGGAAATTCCATTGGGTAAAGAATCCCGATAAC 381 0 91 .......................................................................................... 180 R P K D F Y K P E F Y T G G W A D I Q V P G N W E R Q G Y G Query_1 382 CGTCCGAAGGATTTTTATAAACCGGAGTTTTATACAGGAGGATGGGCGGATATTCAGGTTCCCGGCAATTGGGAACGCCAAGGATATGGA 471 0 181 .......................................................................................... 270 0 1742 ........... 1752 T A I Y V N E T Y E F D D K L F H F K K N P P L V P Y K E N Query_1 472 ACAGCTATTTATGTAAACGAAACCTATGAGTTCGATGACAAACTGTTCCATTTTAAAAAGAATCCGCCTTTAGTTCCATATAAAGAGAAT 561 0 271 .......................................................................................... 360 E V G S Y R R T F T I P A D W E D R R V V I C C E G V I S F Query_1 562 GAAGTCGGTTCCTACAGACGTACCTTTACAATTCCTGCCGATTGGGAAGACCGCCGTGTGGTTATCTGTTGTGAAGGAGTGATTTCTTTT 651 0 361 .......................................................................................... 450 Y Y I W V N G H L L G Y N Q G S K T P A E W D I T D K L K K Query_1 652 TACTATATTTGGGTGAACGGACATCTGTTGGGCTATAATCAAGGTTCAAAGACACCGGCCGAATGGGATATCACTGATAAATTGAAGAAA 741 0 451 .......................................................................................... 540 G E N T V A L E V Y R W S A G S Y L E C Q D M W R L S G I E Query_1 742 GGTGAGAATACGGTGGCATTGGAGGTTTACCGTTGGAGCGCCGGATCGTATTTGGAGTGCCAGGATATGTGGCGGTTGAGTGGTATTGAA 831 0 541 .......................................................................................... 630 R D V Y L Y S T P K Q Y I A D Y K V T S S L D K Q Q Y K E G Query_1 832 CGTGATGTGTATCTTTACAGCACTCCTAAACAGTATATAGCCGATTATAAAGTAACCTCTTCTTTAGACAAACAACAATATAAAGAAGGT 921 0 631 .......................................................................................... 720 0 2040 ........... 2030 I F G L E A T I E G P S T S M T T F A Y R L E D K T G K A V Query_1 922 ATTTTCGGTTTGGAAGCTACCATTGAAGGGCCTTCTACTTCCATGACTACCTTTGCTTACCGACTGGAAGATAAAACCGGTAAGGCGGTT 1011 0 721 .......................................................................................... 810 L Q G E Y P I K S K G L S H F L K L D E Q S L P D V E V W S Query_1 1012 CTGCAAGGTGAATATCCTATCAAATCGAAGGGATTGAGCCATTTCTTGAAGTTGGATGAACAAAGTTTGCCGGATGTGGAAGTCTGGAGT 1101 0 811 .......................................................................................... 900 A E H P N L Y T L I L E L K D V T G K V T E L T G C E V G F Query_1 1102 GCTGAGCATCCGAATCTTTATACACTGATCTTGGAATTGAAAGATGTAACAGGTAAAGTAACGGAACTTACCGGATGTGAAGTAGGTTTC 1191 0 901 .......................................................................................... 990 R T S E I K D G R F C I N G V P V L V K G T N R H E H S Q L Query_1 1192 CGCACTTCGGAAATTAAGGACGGACGTTTTTGCATCAATGGTGTGCCGGTTTTGGTAAAGGGAACGAACCGTCATGAACATTCCCAATTA 1281 0 991 .......................................................................................... 1080 G R T V S K E L M E K D I K L M K Q H N I N T V R N S H Y P Query_1 1282 GGACGGACTGTAAGCAAGGAACTGATGGAAAAAGATATCAAATTGATGAAACAGCATAATATCAATACGGTCCGCAATTCGCACTACCCT 1371 0 1081 .......................................................................................... 1170 T H P Y W Y Q L C D R Y G L Y M I D E A N I E S H G M G Y G Query_1 1372 ACCCATCCTTATTGGTATCAGCTTTGTGATCGTTATGGACTTTATATGATAGATGAAGCTAATATTGAATCGCATGGCATGGGGTATGGA 1461 0 1171 .......................................................................................... 1260 P A S L A K D S T W L P A H M D R T Q R M Y E R S K N H P A Query_1 1462 CCTGCTTCATTGGCCAAAGATTCCACTTGGTTGCCTGCCCACATGGATCGTACCCAACGTATGTATGAGCGTTCCAAGAATCATCCGGCT 1551 0 1261 .......................................................................................... 1350 I V I W S L G N Q A G N G I N F E R T Y D W M K S V E K S R Query_1 1552 ATCGTTATCTGGTCATTGGGGAATCAGGCCGGAAACGGTATTAACTTCGAACGTACATATGACTGGATGAAGTCAGTGGAGAAAAGTCGT 1641 0 1351 ........................G.A............................................................... 1440 P V Q Y E R A E Q N Y N T D I Y C R M Y R S V E E L M A Y A Query_1 1642 CCTGTACAATATGAACGGGCCGAACAGAATTATAATACCGATATCTATTGCCGTATGTATCGCAGCGTGGAAGAACTCATGGCCTATGCC 1731 0 1441 .......................................................................................... 1530 R Q T E P K V Y R P F I M T E Y L H T M G N S G G G L K E Y Query_1 1732 CGCCAGACAGAACCTAAAGTTTATCGTCCTTTCATCATGACAGAGTATCTGCATACTATGGGTAATAGTGGCGGAGGATTAAAGGAATAT 1821 0 1531 .......................................................................................... 1620 0 2288 ........... 2298 M H V F E T E P I V Q G G C I W D W V D Q S F R E I D K D G Query_1 1822 ATGCATGTATTCGAAACCGAACCGATTGTTCAGGGAGGTTGTATCTGGGATTGGGTGGATCAATCTTTCCGCGAGATAGATAAGGATGGT 1911 0 1621 .......................................................................................... 1710 K W Y W S Y G G D Y G P K D V P S F G N F C C N G L V N A V Query_1 1912 AAATGGTATTGGAGTTATGGTGGGGATTATGGTCCGAAGGATGTGCCCAGTTTTGGCAATTTCTGTTGTAACGGGTTGGTCAATGCAGTC 2001 0 1711 .......................................................................................... 1800 0 182 ........... 192 R E P H P H L I E V K K E Y Q Y I K S V L T D P K K L T V E Query_1 2002 CGCGAACCGCATCCGCACCTCATTGAAGTGAAGAAGGAGTATCAATATATCAAATCGGTACTGACCGATCCTAAGAAGCTGACAGTGGAA 2091 0 1801 .......................................................................................... 1890 V K N W Y D F T N L N A Y T L H W Q V M G D D G K V I A E G Query_1 2092 GTCAAGAACTGGTATGACTTTACCAATTTGAATGCCTATACGTTGCACTGGCAGGTCATGGGGGATGATGGGAAAGTGATAGCAGAAGGA 2181 0 1891 .......................................................................................... 1980 T R K A D C A P H E A V T F S L G A V K L P S T I R E A Query_1 2182 ACTCGAAAAGCAGACTGTGCCCCTCAT-GAGGC-TGTAACATTCTCTTTGGGTGCTGTAAAGTTGCCTTCTACGATTCGTGAGGC-TTA- 2267 0 1981 ...........................-.....-...................................................-...- 2066 0 653 ........... 643 1 1159 ...T....A.....C..A..NN.CT....A...N........AG.TN...TNNGN...N.N...NNN...N 1089 Y L N L S W T P D K A T P F I G T H D E V A Y D Q F V Query_1 2268 TTTGAA-TCTGT-CTTGGACGCCTGAT--AAGGCAACTCCCTTTATTGG-AACTC-ATGACG-AAG-TGGCC-TATGACCAGTTTGTACT 2348 0 2067 ......-.....-..............--....................-.....-......-...-.....-................. 2147 0 2163 ...........-C.GA.-.GT..A-..C-...T.-ATA.G...C..C..CA. 2117 1 1088 ......AN.N..A.......N.NN...TA...........NN..N....G..N..N....A.N...N.....C...........-..... 1000 L S A N K G Y R A P E I K L A D K V K I D I D P A T G A Query_1 2349 GTCTGCCAAT--AAAGGGTATCGTGCGCCGGAAATCAAATTGGCTGATAA-GGTGAAG-ATAGATATTGA-TCCTGCAACC-GGCGCTTT 2432 0 2148 ..........--......................................-.......-...........-..........-........ 2231 0 2116 .AG.T.....--...... 2101 0 2257 .... 2254 1 999 ....N.....TA......................................A.......N...........A..........N........ 910 L R S Y I Y K G K E M L S S P V V L S L Y R P V T D N D S Query_1 2433 ACGCTCTTATAT-ATATAA-AG-GTAAAGAGATGCTTTCATCTCCTGTAGTTCTCAGCCTTTATCGTCCTGTTACGGACAATGACAGCAG 2519 0 2232 ............-......-..-................................................................... 2318 0 2253 ..-..-......-......G..C...... 2228 0 1550 ........... 1560 1 909 ............T......-..A......................................-............................ 822 R E K T G G A K V W R K E G L D N M I Q K A T F V K A S E T Query_1 2520 GGAAAAAACAGGAGGAGCCAAAGTTTGGAGGAAAGAAGGTTTGGATAATATGATTCAAAAAGCTA-CTTTTGTCAAAGCATCTGAAACAG 2608 0 2319 .................................................................-........................ 2407 1 821 .......-.........................................................A........................ 733 G G K A E V E L W N A K G V K L G M A T F V Y T L Q S N G Query_1 2609 GTGGTAAAGCGGAAGTAGAGTTATGGAATGCTAAA-GGCGTAAAGTT-GGGAATGGCTACCTTTGTATATACCTTGCAGAGCAATGGTGC 2696 0 2408 ...................................-...........-.......................................... 2495 1 732 ...................................A...........T.......................................... 643 A L K V K T T F L P D T S A V T S L A R V G L A F E M P Y A Query_1 2697 ATTAAAAGTAAAGACAACTTTCTTGCCGGATACTTCTGCTGTTACTTCATTGGCTAGAGTGGGCCTGGCATTTGAAATGCCGTATGCCTA 2786 0 2496 .......................................................................................... 2585 1 642 .......................................................................................... 553 Y N K V S Y L G R G E H E T Y I D R N Q S G C I G I Y H T D Query_1 2787 TAACAAGGTTTCTTATTTGGGACGTGGTGAACATGAAACTTATATAGACCGTAATCAGTCTGGATGTATCGGAATTTACCATACAGATGC 2876 0 2586 .......................................................................................... 2675 1 552 .......................................................................................... 463 A E R M F H Y Y V K P Q A T G N R T D V R W M E L A D E A G Query_1 2877 AGAACGTATGTTCCATTATTATGTGAAGCCCCAGGCTACAGGAAATCGTACGGATGTGCGTTGGATGGAATTGGCGGATGAGGCAGGAGA 2966 0 2676 .......................................................................................... 2765 1 462 .......................................................................................... 373 E G L S F R S D K V F Q F C V I P F T D S N V D K A T H I N Query_1 2967 AGGTTTGTCTTTCCGTTCGGATAAAGTGTTTCAGTTCTGTGTTATTCCATTTACGGATTCTAATGTAGATAAAGCTACTCATATTAATAA 3056 0 2766 .......................................................................................... 2855 1 372 .......................................................................................... 283 K L E R T G I I N V H L D A E Q S G V G T A T C G P G V L P Query_1 3057 ACTTGAGAGAACAGGGATTATAAATGTTCACTTAGATGCGGAACAGTCGGGGGTAGGTACTGCGACTTGTGGTCCGGGGGTATTGCCGCC 3146 0 2856 .......................................................................................... 2945 1 282 .......................................................................................... 193 P Y R V P V E K H T F E F M I C P L K * H W K W I T D P N S Query_1 3147 ATATCGTGTTCCGGTGGAGAAGCATACGTTTGAGTTTATGATTTGTCCATTAAAATAACATTGGAAGTGGATAACGGATCCGAATTCGAG 3236 0 2946 ....................................................... 3000 1 192 .......................................................................................... 103 S S V D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 3237 CTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAG 3319 1 102 ...............NNN....................................................N..N....AN... 20 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 13965336 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,173 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200069.109 (CMR200069.20-1019.P(1019).E544Q.pMCSG68)
QQQPEWQSQYAVGLNKLAPHTYVWPYQTISDIRNGDYESSPYYMSLNGSWKFHWVKNPDNRPKDFYKPEFYTGGWADIQVPGNWERQGYGTAIYVNETYEFDDKLFHFKKNPPLVPYKENEVGSYRRTFTIPADWEDRRVVICCEGVISFYYIWVNGHLLGYNQGSKTPAEWDITDKLKKGENTVALEVYRWSAGSYLECQDMWRLSGIERDVYLYSTPKQYIADYKVTSSLDKQQYKEGIFGLEATIEGPSTSMTTFAYRLEDKTGKAVLQGEYPIKSKGLSHFLKLDEQSLPDVEVWSAEHPNLYTLILELKDVTGKVTELTGCEVGFRTSEIKDGRFCINGVPVLVKGTNRHEHSQLGRTVSKELMEKDIKLMKQHNINTVRNSHYPTHPYWYQLCDRYGLYMIDEANIESHGMGYGPASLAKDSTWLPAHMDRTQRMYERSKNHPAIVIWSLGNEAGNGINFERTYDWMKSVEKSRPVQYERAEQNYNTDIYCRMYRSVEELMAYARQTEPKVYRPFIMTEYLHTMGNSGGGLKEYMHVFETEPIVQGGCIWDWVDQSFREIDKDGKWYWSYGGDYGPKDVPSFGNFCCNGLVNAVREPHPHLIEVKKEYQYIKSVLTDPKKLTVEVKNWYDFTNLNAYTLHWQVMGDDGKVIAEGTRKADCAPHEAVTFSLGAVKLPSTIREAYLNLSWTPDKATPFIGTHDEVAYDQFVLSANKGYRAPEIKLADKVKIDIDPATGALRSYIYKGKEMLSSPVVLSLYRPVTDNDSREKTGGAKVWRKEGLDNMIQKATFVKASETGGKAEVELWNAKGVKLGMATFVYTLQSNGALKVKTTFLPDTSAVTSLARVGLAFEMPYAYNKVSYLGRGEHETYIDRNQSGCIGIYHTDAERMFHYYVKPQATGNRTDVRWMELADEAGEGLSFRSDKVFQFCVIPFTDSNVDKATHINKLERTGIINVHLDAEQSGVGTATCGPGVLPPYRVPVEKHTFEFMICPLK
CAACAACAACCCGAATGGCAGAGCCAATATGCTGTCGGACTGAACAAACTGGCCCCTCATACTTATGTGTGGCCTTATCAAACCATATCTGATATCCGTAACGGAGATTATGAATCTTCTCCTTATTATATGAGTCTGAACGGTAGTTGGAAATTCCATTGGGTAAAGAATCCCGATAACCGTCCGAAGGATTTTTATAAACCGGAGTTTTATACAGGAGGATGGGCGGATATTCAGGTTCCCGGCAATTGGGAACGCCAAGGATATGGAACAGCTATTTATGTAAACGAAACCTATGAGTTCGATGACAAACTGTTCCATTTTAAAAAGAATCCGCCTTTAGTTCCATATAAAGAGAATGAAGTCGGTTCCTACAGACGTACCTTTACAATTCCTGCCGATTGGGAAGACCGCCGTGTGGTTATCTGTTGTGAAGGAGTGATTTCTTTTTACTATATTTGGGTGAACGGACATCTGTTGGGCTATAATCAAGGTTCAAAGACACCGGCCGAATGGGATATCACTGATAAATTGAAGAAAGGTGAGAATACGGTGGCATTGGAGGTTTACCGTTGGAGCGCCGGATCGTATTTGGAGTGCCAGGATATGTGGCGGTTGAGTGGTATTGAACGTGATGTGTATCTTTACAGCACTCCTAAACAGTATATAGCCGATTATAAAGTAACCTCTTCTTTAGACAAACAACAATATAAAGAAGGTATTTTCGGTTTGGAAGCTACCATTGAAGGGCCTTCTACTTCCATGACTACCTTTGCTTACCGACTGGAAGATAAAACCGGTAAGGCGGTTCTGCAAGGTGAATATCCTATCAAATCGAAGGGATTGAGCCATTTCTTGAAGTTGGATGAACAAAGTTTGCCGGATGTGGAAGTCTGGAGTGCTGAGCATCCGAATCTTTATACACTGATCTTGGAATTGAAAGATGTAACAGGTAAAGTAACGGAACTTACCGGATGTGAAGTAGGTTTCCGCACTTCGGAAATTAAGGACGGACGTTTTTGCATCAATGGTGTGCCGGTTTTGGTAAAGGGAACGAACCGTCATGAACATTCCCAATTAGGACGGACTGTAAGCAAGGAACTGATGGAAAAAGATATCAAATTGATGAAACAGCATAATATCAATACGGTCCGCAATTCGCACTACCCTACCCATCCTTATTGGTATCAGCTTTGTGATCGTTATGGACTTTATATGATAGATGAAGCTAATATTGAATCGCATGGCATGGGGTATGGACCTGCTTCATTGGCCAAAGATTCCACTTGGTTGCCTGCCCACATGGATCGTACCCAACGTATGTATGAGCGTTCCAAGAATCATCCGGCTATCGTTATCTGGTCATTGGGGAATGAAGCCGGAAACGGTATTAACTTCGAACGTACATATGACTGGATGAAGTCAGTGGAGAAAAGTCGTCCTGTACAATATGAACGGGCCGAACAGAATTATAATACCGATATCTATTGCCGTATGTATCGCAGCGTGGAAGAACTCATGGCCTATGCCCGCCAGACAGAACCTAAAGTTTATCGTCCTTTCATCATGACAGAGTATCTGCATACTATGGGTAATAGTGGCGGAGGATTAAAGGAATATATGCATGTATTCGAAACCGAACCGATTGTTCAGGGAGGTTGTATCTGGGATTGGGTGGATCAATCTTTCCGCGAGATAGATAAGGATGGTAAATGGTATTGGAGTTATGGTGGGGATTATGGTCCGAAGGATGTGCCCAGTTTTGGCAATTTCTGTTGTAACGGGTTGGTCAATGCAGTCCGCGAACCGCATCCGCACCTCATTGAAGTGAAGAAGGAGTATCAATATATCAAATCGGTACTGACCGATCCTAAGAAGCTGACAGTGGAAGTCAAGAACTGGTATGACTTTACCAATTTGAATGCCTATACGTTGCACTGGCAGGTCATGGGGGATGATGGGAAAGTGATAGCAGAAGGAACTCGAAAAGCAGACTGTGCCCCTCATGAGGCTGTAACATTCTCTTTGGGTGCTGTAAAGTTGCCTTCTACGATTCGTGAGGCTTATTTGAATCTGTCTTGGACGCCTGATAAGGCAACTCCCTTTATTGGAACTCATGACGAAGTGGCCTATGACCAGTTTGTACTGTCTGCCAATAAAGGGTATCGTGCGCCGGAAATCAAATTGGCTGATAAGGTGAAGATAGATATTGATCCTGCAACCGGCGCTTTACGCTCTTATATATATAAAGGTAAAGAGATGCTTTCATCTCCTGTAGTTCTCAGCCTTTATCGTCCTGTTACGGACAATGACAGCAGGGAAAAAACAGGAGGAGCCAAAGTTTGGAGGAAAGAAGGTTTGGATAATATGATTCAAAAAGCTACTTTTGTCAAAGCATCTGAAACAGGTGGTAAAGCGGAAGTAGAGTTATGGAATGCTAAAGGCGTAAAGTTGGGAATGGCTACCTTTGTATATACCTTGCAGAGCAATGGTGCATTAAAAGTAAAGACAACTTTCTTGCCGGATACTTCTGCTGTTACTTCATTGGCTAGAGTGGGCCTGGCATTTGAAATGCCGTATGCCTATAACAAGGTTTCTTATTTGGGACGTGGTGAACATGAAACTTATATAGACCGTAATCAGTCTGGATGTATCGGAATTTACCATACAGATGCAGAACGTATGTTCCATTATTATGTGAAGCCCCAGGCTACAGGAAATCGTACGGATGTGCGTTGGATGGAATTGGCGGATGAGGCAGGAGAAGGTTTGTCTTTCCGTTCGGATAAAGTGTTTCAGTTCTGTGTTATTCCATTTACGGATTCTAATGTAGATAAAGCTACTCATATTAATAAACTTGAGAGAACAGGGATTATAAATGTTCACTTAGATGCGGAACAGTCGGGGGTAGGTACTGCGACTTGTGGTCCGGGGGTATTGCCGCCATATCGTGTTCCGGTGGAGAAGCATACGTTTGAGTTTATGATTTGTCCATTAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,486 total letters Query= CMR200069.109 Length=3405 Score E Sequences producing significant alignments: (Bits) Value 0_wt 5405 0.0 1_04B_B7_CMR200069.109_T7term.seq 1934 0.0 2_07B_B7_CMR200069.109_T7S.seq 1887 0.0 Q F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 55 CAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTG 144 2 16 ..T....................................................................................... 105 W S H P Q F E K G T E N L Y F Q S N A Q Q Q P E W Q S Q Y A Query_1 145 TGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAACAACAACCCGAATGGCAGAGCCAATATGCT 234 0 1 ................................. 33 2 106 .......................................................................................... 195 V G L N K L A P H T Y V W P Y Q T I S D I R N G D Y E S S P Query_1 235 GTCGGACTGAACAAACTGGCCCCTCATACTTATGTGTGGCCTTATCAAACCATATCTGATATCCGTAACGGAGATTATGAATCTTCTCCT 324 0 34 .......................................................................................... 123 2 196 .......................................................................................... 285 Y Y M S L N G S W K F H W V K N P D N R P K D F Y K P E F Y Query_1 325 TATTATATGAGTCTGAACGGTAGTTGGAAATTCCATTGGGTAAAGAATCCCGATAACCGTCCGAAGGATTTTTATAAACCGGAGTTTTAT 414 0 124 .......................................................................................... 213 0 1742 ........... 1752 2 286 ..................................................................................G....... 375 T G G W A D I Q V P G N W E R Q G Y G T A I Y V N E T Y E F Query_1 415 ACAGGAGGATGGGCGGATATTCAGGTTCCCGGCAATTGGGAACGCCAAGGATATGGAACAGCTATTTATGTAAACGAAACCTATGAGTTC 504 0 214 .......................................................................................... 303 2 376 .......................................................................................... 465 D D K L F H F K K N P P L V P Y K E N E V G S Y R R T F T I Query_1 505 GATGACAAACTGTTCCATTTTAAAAAGAATCCGCCTTTAGTTCCATATAAAGAGAATGAAGTCGGTTCCTACAGACGTACCTTTACAATT 594 0 304 .......................................................................................... 393 2 466 .......................................................................................... 555 P A D W E D R R V V I C C E G V I S F Y Y I W V N G H L L G Query_1 595 CCTGCCGATTGGGAAGACCGCCGTGTGGTTATCTGTTGTGAAGGAGTGATTTCTTTTTACTATATTTGGGTGAACGGACATCTGTTGGGC 684 0 394 .......................................................................................... 483 2 556 .......................................................................................... 645 Y N Q G S K T P A E W D I T D K L K K G E N T V A L E V Y R Query_1 685 TATAATCAAGGTTCAAAGACACCGGCCGAATGGGATATCACTGATAAATTGAAGAAAGGTGAGAATACGGTGGCATTGGAGGTTTACCGT 774 0 484 .......................................................................................... 573 2 646 .......................................................................................... 735 W S A G S Y L E C Q D M W R L S G I E R D V Y L Y S T P K Query_1 775 TGGAGCGCCGGATCGTATTTGGAGTGCCAGGATATGTGGCGGTTGAGTGGTATTGAACGTGATGTGTATC-TTTACAGCACTCCTAAACA 863 0 574 ......................................................................-................... 662 0 2040 .-.......... 2030 2 736 ......................................................................T................... 825 Q Y I A D Y K V T S S L D K Q Q Y K E G I F G L E A T I E G Query_1 864 GTATATAGCCGATTATAAAGTAACCTCTTCTTTAGACAAACAACAATATAAAG-AAGGTATTTTCGGTTTGGAAG-CTACCATTGAAGGG 951 0 663 .....................................................-.....................-.............. 750 2 826 .....................................................A.....................C.............. 915 P S T S M T T F A Y R L E D K T G K A V L Q G E Y P I K S Query_1 952 CCTT-CTACTTCCATGACTACC-TTTGCTTACCGACTGGAAGATAAAACCGGTAAGGCGGTTCTGCAAGGTGAATATCCTATCAAATCGA 1039 0 751 ....-.................-................................................................... 838 2 916 T...N............N....T...................................T........N...................... 1005 K G L S H F L K L D E Q S L P D V E V W S A E H P N L Y T Query_1 1040 AGGGATTG-AGCCATTTCTTGAAGTTGGATGAACAAAGTTTGCCGGATGTGGAAGT-CTGGAGTGCTGAGCATCCGAATCTTTATACAC- 1126 0 839 ........-...............................................-................................- 925 2 1006 ........A.....-.........-.........-..............GT.....C...N........N...................C 1092 L I L E L K D V T G K V T E L T G C E V G F R T S E I K Query_1 1127 -TGATCTTGGAATTGAAAGATGTAACAGGTAAAGTAACGG-AACTTA--CCGGATGTGAAGTA-GGTTTCCGCACTTCGGAAATTAAGGA 1211 0 926 -.......................................-......--..............-.......................... 1010 2 1093 TG...........N...GN.NTAT......-...N.....A......CT.G.....NN.....N........N....TC.G.....N.N. 1181 D G R F C I N G V P V L V K G T N R H E H S Q L G R T V S K Query_1 1212 CGGA-CGTTTTTGCATCAATGGTGTG-CCGGTTTTGGTAAAGGGAACGAACCGTCATGAACATTCCCAATTAGGACGGACTGTAAGCAAG 1299 0 1011 ....-.....................-............................................................... 1098 2 1182 ....N.......TGCN.N.N...N..T....A..N...-.N..NNCNN..N.....N.... 1241 E L M E K D I K L M K Q H N I N T V R N S H Y P T H P Y W Y Query_1 1300 GAACTGATGGAAAAAGATATCAAATTGATGAAACAGCATAATATCAATACGGTCCGCAATTCGCACTACCCTACCCATCCTTATTGGTAT 1389 0 1099 .......................................................................................... 1188 Q L C D R Y G L Y M I D E A N I E S H G M G Y G P A S L A K Query_1 1390 CAGCTTTGTGATCGTTATGGACTTTATATGATAGATGAAGCTAATATTGAATCGCATGGCATGGGGTATGGACCTGCTTCATTGGCCAAA 1479 0 1189 .......................................................................................... 1278 D S T W L P A H M D R T Q R M Y E R S K N H P A I V I W S L Query_1 1480 GATTCCACTTGGTTGCCTGCCCACATGGATCGTACCCAACGTATGTATGAGCGTTCCAAGAATCATCCGGCTATCGTTATCTGGTCATTG 1569 0 1279 .......................................................................................... 1368 G N E A G N G I N F E R T Y D W M K S V E K S R P V Q Y E R Query_1 1570 GGGAATGAAGCCGGAAACGGTATTAACTTCGAACGTACATATGACTGGATGAAGTCAGTGGAGAAAAGTCGTCCTGTACAATATGAACGG 1659 0 1369 .......................................................................................... 1458 A E Q N Y N T D I Y C R M Y R S V E E L M A Y A R Q T E P K Query_1 1660 GCCGAACAGAATTATAATACCGATATCTATTGCCGTATGTATCGCAGCGTGGAAGAACTCATGGCCTATGCCCGCCAGACAGAACCTAAA 1749 0 1459 .......................................................................................... 1548 V Y R P F I M T Q Y L H T M G N S G G G L K E Y M H V F E T Query_1 1750 GTTTATCGTCCTTTCATCATGACACAGTATCTGCATACTATGGGTAATAGTGGCGGAGGATTAAAGGAATATATGCATGTATTCGAAACC 1839 0 1549 ........................G................................................................. 1638 0 2288 ........... 2298 1 844 ........... 834 E P I V Q G G C I W D W V D Q S F R E I D K D G K W Y W S Y Query_1 1840 GAACCGATTGTTCAGGGAGGTTGTATCTGGGATTGGGTGGATCAATCTTTCCGCGAGATAGATAAGGATGGTAAATGGTATTGGAGTTAT 1929 0 1639 .......................................................................................... 1728 G G D Y G P K D V P S F G N F C C N G L V N A V R E P H P H Query_1 1930 GGTGGGGATTATGGTCCGAAGGATGTGCCCAGTTTTGGCAATTTCTGTTGTAACGGGTTGGTCAATGCAGTCCGCGAACCGCATCCGCAC 2019 0 1729 .......................................................................................... 1818 0 182 ........... 192 2 344 ........... 354 L I E V K K E Y Q Y I K S V L T D P K K L T V E V K N W Y D Query_1 2020 CTCATTGAAGTGAAGAAGGAGTATCAATATATCAAATCGGTACTGACCGATCCTAAGAAGCTGACAGTGGAAGTCAAGAACTGGTATGAC 2109 0 1819 .......................................................................................... 1908 F T N L N A Y T L H W Q V M G D D G K V I A E G T R K A D Query_1 2110 TTTACCAATTTGAATGCCTATACGTTGCACTGGCAGGTCATGGGGGATGATGGGAAAGTGATAGCAGAAGGAACTCGAAAAGCAGA-CTG 2198 0 1909 ......................................................................................-... 1997 1 1217 ....A.......A.NNNNNNG...T...N.........NC--.....N.--..NNN........NNN.NNNN.........NN... 1136 C A P H E A V T F S L G A V K L P S T I R E A Y L N L S W T Query_1 2199 TGCCCCTCATGAGGCTGTAACATTCTCTTTGGGTGCTGTAAAGTTGCCTTCTACGATTC--GTGAGGCTTATTTGAATCTGTCTTGGACG 2286 0 1998 ...........................................................--............................. 2085 0 653 ........... 643 1 1135 NN...T......NNTGT...............N.........................TCG............-......N......... 1047 P D K A T P F I G T H D E V A Y D Q F V L S A N K G Y R A Query_1 2287 CCTGATAAGGCAACTCCCTTTATTGGAACTCATGACGAAGTGGCC-TATGACCAGTTTGTACTGTCTGCCAATAAAGG-GTATCGTGCGC 2374 0 2086 .............................................-................................-........... 2173 0 2163 ...........C.GA..GT..A..C...T.-ATA.G...C..C..CA..AG.T..........-. 2101 1 1046 .............NN........-.N...................N....N................N..........N........... 958 1 970 ..........N.GA..GT..A..C...N.A..N.G...C..C.. 1013 P E I K L A D K V K I D I D P A T G A L R S Y I Y K G K E Query_1 2375 CGGAAATCAAATTGGCTGATAAGGTGAAGATAGATATTGATCCTGCAACCGGCGCTTTACGCTCTTATATATATAA-AG-GTAAAGAGAT 2462 0 2174 ............................................................................-..-.......... 2261 0 2257 ......-..-............G..C...... 2228 1 957 ............................................................................-..-.......... 870 1 874 ......-..-............G..C...... 903 M L S S P V V L S L Y R P V T D N D S R E K T G G A K V W R Query_1 2463 GCTTTCATCTCCTGTAGTTCTCAGCCTTTATCGTCCTGTTACGGACAATGACAGCAGGGAAAAAACAGGAGGAGCCAAAGTTTGGAGGAA 2552 0 2262 .......................................................................................... 2351 0 1550 ........... 1560 1 869 ....-.............................................................................-....... 782 K E G L D N M I Q K A T F V K A S E T G G K A E V E L W N A Query_1 2553 AGAAGGTTTGGATAATATGATTCAAAAAGCTACTTTTGTCAAAGCATCTGAAACAGGTGGTAAAGCGGAAGTAGAGTTATGGAATGCTAA 2642 0 2352 .......................................................................................... 2441 1 781 .......................................................................................... 692 K G V K L G M A T F V Y T L Q S N G A L K V K T T F L P D T Query_1 2643 AGGCGTAAAGTTGGGAATGGCTACCTTTGTATATACCTTGCAGAGCAATGGTGCATTAAAAGTAAAGACAACTTTCTTGCCGGATACTTC 2732 0 2442 .......................................................................................... 2531 1 691 .......................................................................................... 602 S A V T S L A R V G L A F E M P Y A Y N K V S Y L G R G E H Query_1 2733 TGCTGTTACTTCATTGGCTAGAGTGGGCCTGGCATTTGAAATGCCGTATGCCTATAACAAGGTTTCTTATTTGGGACGTGGTGAACATGA 2822 0 2532 .......................................................................................... 2621 1 601 .......................................................................................... 512 E T Y I D R N Q S G C I G I Y H T D A E R M F H Y Y V K P Q Query_1 2823 AACTTATATAGACCGTAATCAGTCTGGATGTATCGGAATTTACCATACAGATGCAGAACGTATGTTCCATTATTATGTGAAGCCCCAGGC 2912 0 2622 .......................................................................................... 2711 1 511 .......................................................................................... 422 A T G N R T D V R W M E L A D E A G E G L S F R S D K V F Q Query_1 2913 TACAGGAAATCGTACGGATGTGCGTTGGATGGAATTGGCGGATGAGGCAGGAGAAGGTTTGTCTTTCCGTTCGGATAAAGTGTTTCAGTT 3002 0 2712 .......................................................................................... 2801 1 421 .......................................................................................... 332 F C V I P F T D S N V D K A T H I N K L E R T G I I N V H L Query_1 3003 CTGTGTTATTCCATTTACGGATTCTAATGTAGATAAAGCTACTCATATTAATAAACTTGAGAGAACAGGGATTATAAATGTTCACTTAGA 3092 0 2802 .......................................................................................... 2891 1 331 .......................................................................................... 242 D A E Q S G V G T A T C G P G V L P P Y R V P V E K H T F E Query_1 3093 TGCGGAACAGTCGGGGGTAGGTACTGCGACTTGTGGTCCGGGGGTATTGCCGCCATATCGTGTTCCGGTGGAGAAGCATACGTTTGAGTT 3182 0 2892 .......................................................................................... 2981 1 241 .......................................................................................... 152 F M I C P L K * H W K W I T D P N S S S V D K L A A A L E H Query_1 3183 TATGATTTGTCCATTAAAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcacca 3272 0 2982 ................... 3000 1 151 .......................................................................................... 62 H H H H H * D P A A N K A R K E Query_1 3273 ccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAA 3318 1 61 .............................................. 16 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 18398184 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 5,486 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200071.104 (CMR200071.21-875.P(875).E417A.pMCSG68)
SEISITDSWKYKAENDERFSSMDWNDSDWVTVDLPHTWNAGDVIDEQRGYRRGISWYRKKLFIPSEARDKKITLRFDGVASKADVYLNGKLLQTHLGAYTAFGVDITDICEVGKENLLAVKVDNSSSLGEILPSVSGDFSIFGGIYRRVFLQWTERVHFVTEPYAAVPVRIQTPEVSVSEASMQIVAFLKNDFTDTKHVHVNVFLCDEMNRIVKEKQLKLKLIPGRKYPISTSVGRIENPHLWSPELPYLYTVKVQVCDAKNGEMYQEVISPVGFRWFSVDKTGFYLNGKYLKLRGAARHQDYAGLGTAIPVEMNRRDMRLLKEMGANFVRISHYPQDPEIYRACDELGLIVWSEICVVNEVRKNTKFAHNCKEMLKEMILQNYNHPSVVLWGAMNELWDYHKQAIVLARELEALKKELDPYRLSCVAFHAFTWEKPYTQSSKEMFNISDVNGVNVYESWYQGDSATIAPMFDKFCSYSTAKPRFLSEFGAGSDERIHSYTPRTFDFTPEFQLDFNRRYINEMEKRPDYIGYSIWNLVDFQVDGRGDSKPNLNQKGMLTEDRRKKEIYYYCQARWSDIPMIHIAGADWTKRVEICDDSINVRKISVFSNQKTVELIHNGKSLGVREVVNGEAVFVVPFINGENLLDARSGALSDRLKIQMRLLSSRLTDSDVLLDGLCINLGQEHCYFIDPQLQEIWIPDKPYTKGSWGYMDGKPFNSWPGSSHDGVRYGVGADIKNTFLEPLFQTFLIGTTCYRLDVPDGVYEIGFYFTEPFSKDERKNIVRTGVSAEGQRVFDVSVNGEKLIESLNLADSYGEQTAVVKTLVVNVRNHEGLEILLSPQKGQGVISGLKVKKIR
TCTGAAATTTCTATAACAGATTCATGGAAGTATAAAGCAGAGAATGATGAACGTTTTTCCAGTATGGATTGGAATGATTCTGATTGGGTAACTGTAGATTTGCCCCATACTTGGAATGCCGGAGATGTGATTGACGAGCAACGGGGATATAGGCGCGGCATATCTTGGTATAGGAAGAAGTTGTTCATTCCTTCAGAGGCACGGGATAAGAAAATAACTCTTCGTTTTGATGGAGTGGCCAGCAAGGCAGACGTCTATTTAAATGGGAAATTATTGCAAACTCATTTGGGGGCATATACCGCTTTCGGAGTTGATATAACTGATATCTGTGAAGTTGGAAAGGAAAATCTGTTGGCGGTAAAAGTGGATAATAGTTCTTCTTTGGGGGAAATCCTTCCATCGGTTTCCGGAGATTTTTCTATTTTTGGGGGTATTTATCGCAGGGTGTTCTTGCAATGGACGGAAAGAGTGCATTTTGTGACAGAACCTTATGCAGCTGTTCCAGTAAGGATACAAACCCCTGAAGTGTCGGTATCTGAGGCGTCAATGCAAATTGTGGCTTTTCTTAAAAATGACTTTACCGATACAAAGCACGTGCACGTCAATGTTTTTCTTTGCGATGAAATGAATCGGATTGTGAAAGAGAAACAGTTGAAACTGAAACTGATTCCAGGCAGGAAATATCCTATATCCACTTCTGTAGGAAGGATAGAGAATCCTCATTTGTGGTCTCCTGAATTACCTTATTTATATACGGTAAAAGTGCAGGTATGCGATGCCAAGAATGGAGAAATGTATCAAGAAGTAATTTCTCCGGTTGGATTCAGATGGTTTTCGGTGGACAAGACAGGCTTTTATCTGAACGGGAAGTATCTTAAATTGAGGGGAGCTGCACGGCATCAGGATTATGCAGGTTTGGGAACGGCTATTCCCGTTGAAATGAATCGTAGGGATATGCGGTTGCTGAAAGAAATGGGAGCTAATTTTGTGCGTATCAGTCATTATCCTCAAGATCCGGAAATATATCGAGCCTGTGACGAATTGGGGTTGATAGTATGGTCTGAAATTTGTGTTGTGAATGAAGTGAGAAAAAATACGAAATTCGCTCATAACTGCAAAGAAATGTTGAAAGAGATGATTCTTCAGAACTACAATCATCCATCGGTGGTACTATGGGGGGCTATGAATGAATTATGGGATTACCATAAGCAAGCTATTGTGTTGGCTAGGGAATTGGAGGCTTTGAAAAAAGAGCTGGATCCTTATCGTCTTTCTTGTGTCGCCTTTCATGCTTTTACTTGGGAGAAACCCTATACACAGTCTAGTAAGGAGATGTTTAATATTTCGGATGTTAACGGGGTAAATGTATATGAATCTTGGTATCAGGGAGATTCTGCTACGATTGCACCTATGTTTGATAAATTTTGTAGTTATTCTACTGCAAAACCTCGTTTTTTGTCGGAATTTGGAGCAGGAAGCGATGAACGTATCCATTCTTATACTCCGCGTACATTTGATTTTACTCCGGAATTTCAATTGGATTTTAATCGCAGATATATCAATGAAATGGAGAAACGTCCGGATTATATAGGTTATTCTATTTGGAATTTGGTTGATTTTCAGGTGGACGGTCGTGGTGATAGCAAGCCTAATCTGAATCAAAAAGGAATGTTGACAGAGGATCGTCGGAAGAAAGAGATTTATTATTACTGTCAGGCTCGTTGGTCGGACATTCCGATGATTCATATTGCCGGGGCCGATTGGACGAAAAGGGTGGAAATTTGTGATGATAGTATAAATGTGCGTAAGATCAGTGTTTTTTCTAATCAGAAAACGGTGGAGTTAATTCATAATGGAAAGTCTTTGGGAGTGCGTGAAGTGGTAAATGGGGAAGCTGTTTTCGTAGTTCCTTTTATAAACGGAGAGAATCTGCTGGATGCCCGAAGTGGGGCACTTTCAGACAGGCTGAAGATACAGATGAGATTGTTATCTTCCCGTCTTACTGACAGTGATGTGCTTTTGGACGGTCTTTGTATTAATTTGGGACAGGAGCATTGTTATTTTATAGACCCGCAGCTTCAGGAAATATGGATACCCGATAAACCTTATACAAAGGGAAGTTGGGGATATATGGATGGAAAACCTTTTAACTCCTGGCCGGGATCATCACATGATGGAGTTAGGTATGGAGTAGGGGCTGATATAAAAAATACTTTTTTAGAGCCTCTTTTTCAGACCTTTTTAATTGGTACGACTTGTTACCGGCTGGATGTTCCTGACGGAGTATATGAAATTGGCTTTTATTTCACGGAACCTTTCAGTAAGGATGAACGGAAAAATATTGTGCGTACAGGTGTTTCTGCCGAGGGACAACGTGTATTTGATGTGTCAGTCAATGGAGAAAAATTGATAGAGTCATTGAATCTGGCTGATTCTTATGGCGAGCAGACAGCTGTTGTAAAGACACTTGTGGTCAATGTGCGGAATCATGAAGGACTGGAGATTCTGCTTTCTCCTCAAAAAGGACAAGGTGTTATTAGCGGATTAAAAGTAAAGAAAATACGA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,905 total letters Query= CMR200071.104 Length=2970 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4617 0.0 1_04C_C7_CMR200071.104_T7term.seq 1597 0.0 2_07C_C7_CMR200071.104_T7S.seq 1559 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 58 TTCCCCT-CTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTG 146 2 19 .......T...N.............................................................................. 108 W S H P Q F E K G T E N L Y F Q S N A S E I S I T D S W K Y Query_1 147 GTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCTCTGAAATTTCTATAACAGATTCATGGAAGTATAA 236 0 1 ................................... 35 2 109 .......................................................................................... 198 K A E N D E R F S S M D W N D S D W V T V D L P H T W N A G Query_1 237 AGCAGAGAATGATGAACGTTTTTCCAGTATGGATTGGAATGATTCTGATTGGGTAACTGTAGATTTGCCCCATACTTGGAATGCCGGAGA 326 0 36 .......................................................................................... 125 2 199 .......................................................................................... 288 D V I D E Q R G Y R R G I S W Y R K K L F I P S E A R D K K Query_1 327 TGTGATTGACGAGCAACGGGGATATAGGCGCGGCATATCTTGGTATAGGAAGAAGTTGTTCATTCCTTCAGAGGCACGGGATAAGAAAAT 416 0 126 .......................................................................................... 215 2 289 .......................................................................................... 378 I T L R F D G V A S K A D V Y L N G K L L Q T H L G A Y T A Query_1 417 AACTCTTCGTTTTGATGGAGTGGCCAGCAAGGCAGACGTCTATTTAAATGGGAAATTATTGCAAACTCATTTGGGGGCATATACCGCTTT 506 0 216 .......................................................................................... 305 2 379 .......................................................................................... 468 F G V D I T D I C E V G K E N L L A V K V D N S S S L G E I Query_1 507 CGGAGTTGATATAACTGATATCTGTGAAGTTGGAAAGGAAAATCTGTTGGCGGTAAAAGTGGATAATAGTTCTTCTTTGGGGGAAATCCT 596 0 306 .......................................................................................... 395 2 469 .......................................................................................... 558 L P S V S G D F S I F G G I Y R R V F L Q W T E R V H F V T Query_1 597 TCCATCGGTTTCCGGAGATTTTTCTATTTTTGGGGGTATTTATCGCAGGGTGTTCTTGCAATGGACGGAAAGAGTGCATTTTGTGACAGA 686 0 396 .......................................................................................... 485 2 559 .......................................................................................... 648 E P Y A A V P V R I Q T P E V S V S E A S M Q I V A F L K N Query_1 687 ACCTTATGCAGCTGTTCCAGTAAGGATACAAACCCCTGAAGTGTCGGTATCTGAGGCGTCAATGCAAATTGTGGCTTTTCTTAAAAATGA 776 0 486 .......................................................................................... 575 2 649 ..................................................................................N....... 738 D F T D T K H V H V N V F L C D E M N R I V K E K Q L K L K Query_1 777 CTTTACCGATACAAAGCACGTGCACGTCAATG-TTTTTCTTTGCGATGAAATGAATCGGATTGTGAAAGAGAAACAGTTGAAACTGAAAC 865 0 576 ................................-......................................................... 664 0 933 ..T............ 947 2 739 ................................T......................................-.................. 827 L I P G R K Y P I S T S V G R I E N P H L W S P E L P Y L Query_1 866 TGATTCCAGGCAGGAAATATCCTATATCCACTTCTGTAGGAAGGATAGAGAATCCTCATTTGTGGTC-TCCTGAATTACCTT-ATTTATA 953 0 665 ...................................................................-..............-....... 752 2 828 ...........N..-....................................................T..N...........N.....C. 916 Y T V K V Q V C D A K N G E M Y Q E V I S P V G F R W F S V Query_1 954 TACGGTAAAAGTGCAGGTATGCGATGCCAAGAATGGAGAAATGTATCAAGAAGTAATTTCTCCGGTTGGATTCAGATGGTTTTCGGTGGA 1043 0 753 .......................................................................................... 842 0 356 ........... 366 2 917 .. 918 D K T G F Y L N G K Y L K L R G A A R H Q D Y A G L G T A I Query_1 1044 CAAGACAGGCTTTTATCTGAACGGGAAGTATCTTAAATTGAGGGGAGCTGCACGGCATCAGGATTATGCAGGTTTGGGAACGGCTATTCC 1133 0 843 .......................................................................................... 932 P V E M N R R D M R L L K E M G A N F V R I S H Y P Q D P E Query_1 1134 CGTTGAAATGAATCGTAGGGATATGCGGTTGCTGAAAGAAATGGGAGCTAATTTTGTGCGTATCAGTCATTATCCTCAAGATCCGGAAAT 1223 0 933 .......................................................................................... 1022 0 618 ..A............ 632 2 782 ..A............ 796 I Y R A C D E L G L I V W S E I C V V N E V R K N T K F A H Query_1 1224 ATATCGAGCCTGTGACGAATTGGGGTTGATAGTATGGTCTGAAATTTGTGTTGTGAATGAAGTGAGAAAAAATACGAAATTCGCTCATAA 1313 0 1023 .......................................................................................... 1112 N C K E M L K E M I L Q N Y N H P S V V L W G A M N A L W D Query_1 1314 CTGCAAAGAAATGTTGAAAGAGATGATTCTTCAGAACTACAATCATCCATCGGTGGTACTATGGGGGGCTATGAATGCTTTATGGGATTA 1403 0 1113 .............................................................................AA........... 1202 Y H K Q A I V L A R E L E A L K K E L D P Y R L S C V A F H Query_1 1404 CCATAAGCAAGCTATTGTGTTGGCTAGGGAATTGGAGGCTTTGAAAAAAGAGCTGGATCCTTATCGTCTTTCTTGTGTCGCCTTTCATGC 1493 0 1203 .......................................................................................... 1292 A F T W E K P Y T Q S S K E M F N I S D V N G V N V Y E S W Query_1 1494 TTTTACTTGGGAGAAACCCTATACACAGTCTAGTAAGGAGATGTTTAATATTTCGGATGTTAACGGGGTAAATGTATATGAATCTTGGTA 1583 0 1293 .......................................................................................... 1382 Y Q G D S A T I A P M F D K F C S Y S T A K P R F L S E F G Query_1 1584 TCAGGGAGATTCTGCTACGATTGCACCTATGTTTGATAAATTTTGTAGTTATTCTACTGCAAAACCTCGTTTTTTGTCGGAATTTGGAGC 1673 0 1383 .......................................................................................... 1472 1 185 ............ 174 A G S D E R I H S Y T P R T F D F T P E F Q L D F N R R Y I Query_1 1674 AGGAAGCGATGAACGTATCCATTCTTATACTCCGCGTACATTTGATTTTACTCCGGAATTTCAATTGGATTTTAATCGCAGA--TATATC 1761 0 1473 ..................................................................................--...... 1560 1 1203 .....NTN........-..N....-.....T.NNNC.NNN..N.C. 1160 N E M E K R P D Y I G Y S I W N L V D F Q V D G R G D S Query_1 1762 AATGAAATGGAGAAACG-TCCGGATTATATA-GGTTATTCTATTTGGAA-TTTGGTTGATTTTC-AGGTGG-ACGGTCGTGGT-GATAGC 1845 0 1561 .................-.............-.................-..............-......-...........-...... 1644 1 1159 ....N..N..G...N.NN..G.....NN...N....G.C..C..G.N..AN........N....C.N..N.N..C..N.G..GA....N. 1070 K P N L N Q K G M L T E D R R K K E I Y Y Y C Q A R W Query_1 1846 AAGCCTAATCTGAATC--AAAAAGGAATGTTGA-CAGAGGATCGT-CGGAAGAAA--GAGATTTATTATTAC-TGTCAGGC-TCGTTGGT 1927 0 1645 ................--...............-...........-.........--...............-........-........ 1726 1 1069 C.A..NTT.TN.....NA..............NN......N.NN.T.....N...AGN......N.....C.CN...N...N........ 980 S D I P M I H I A G A D W T K R V E I C D D S I N V Query_1 1928 C-GGACATTCC-GATGATT--CATATTGCCGGGG--CCGATTGGACGAAAA--GGGTGGAAATTT-GTGATG-ATAGTATAAA-TGTGCG 2006 0 1727 .-.........-.......--.............--...............--............-......-..........-...... 1805 1 979 .C.........C..N.N..TC..N...N..C...GC..N.....G......AG............N.N..N.N..........A...... 890 R K I S V F S N Q K T V E L I H N G K S L G V R E V V N Query_1 2007 T-AAGATC-AGTGTTTTTTCT-AATC-AGAAAACGGTGGAGTTAA-TTC-ATAATGGAAAGTCTTTGGGAGTGCGTGAAGTGGTAAATGG 2090 0 1806 .-......-............-....-..................-...-........................................ 1889 1 889 .N......C..........N.N....C..................N...N........................................ 800 G E A V F V V P F I N G E N L L D A R S G A L S D R L K I Q Query_1 2091 GG-AAGCTGTTTT-CGTAGTTCCTTTTATAAACGGAGAGAATCTGCTGGATGCCCGAAGTGGGGCACTTTCAGACAGGCTGAAGATACAG 2178 0 1890 ..-..........-............................................................................ 1977 1 799 ..G..........TN...........................N...N........................................... 710 M R L L S S R L T D S D V L L D G L C I N L G Q E H C Y F Query_1 2179 ATGAGATTGTTATCTT-CCCGTCTTACTGACAGTGATGTGCTTTTGG-ACGGTCTTTGTA-TTAATTTGGGACAGGAGCATTGTTATTTT 2265 0 1978 ................-..............................-............-............................. 2064 1 709 ................T..............................N............A............................. 620 I D P Q L Q E I W I P D K P Y T K G S W G Y M D G K P F N S Query_1 2266 ATAGACCCGCAGCTTCAGGAAATATGGATACCCGATAAACCTTATACAAAGGGAAGTTGGGGATATATGGATGGAAAACCTTTTAACTCC 2355 0 2065 .......................................................................................... 2154 1 619 .......................................................................................... 530 W P G S S H D G V R Y G V G A D I K N T F L E P L F Q T F L Query_1 2356 TGGCCGGGATCATCACATGATGGAGTTAGGTATGGAGTAGGGGCTGATATAAAAAATACTTTTTTAGAGCCTCTTTTTCAGACCTTTTTA 2445 0 2155 .......................................................................................... 2244 1 529 .......................................................................................... 440 I G T T C Y R L D V P D G V Y E I G F Y F T E P F S K D E R Query_1 2446 ATTGGTACGACTTGTTACCGGCTGGATGTTCCTGACGGAGTATATGAAATTGGCTTTTATTTCACGGAACCTTTCAGTAAGGATGAACGG 2535 0 2245 .......................................................................................... 2334 1 439 .......................................................................................... 350 K N I V R T G V S A E G Q R V F D V S V N G E K L I E S L N Query_1 2536 AAAAATATTGTGCGTACAGGTGTTTCTGCCGAGGGACAACGTGTATTTGATGTGTCAGTCAATGGAGAAAAATTGATAGAGTCATTGAAT 2625 0 2335 .......................................................................................... 2424 1 349 .......................................................................................... 260 L A D S Y G E Q T A V V K T L V V N V R N H E G L E I L L S Query_1 2626 CTGGCTGATTCTTATGGCGAGCAGACAGCTGTTGTAAAGACACTTGTGGTCAATGTGCGGAATCATGAAGGACTGGAGATTCTGCTTTCT 2715 0 2425 .......................................................................................... 2514 1 259 .......................................................................................... 170 P Q K G Q G V I S G L K V K K I R * H W K W I T D P N S S S Query_1 2716 CCTCAAAAAGGACAAGGTGTTATTAGCGGATTAAAAGTAAAGAAAATACGATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCC 2805 0 2515 ................................................... 2565 1 169 .......................................................................................... 80 V D K L A A A L E H H H H H H * D P A A N K A R K Query_1 2806 GTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGAT-CCGGCTGCTAACAAAGCCCGAAAG 2880 1 79 .......N.N..NA.....................................C........................ 4 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 14333862 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,905 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200071.105 (CMR200071.21-875.P(875).E508A.pMCSG68)
SEISITDSWKYKAENDERFSSMDWNDSDWVTVDLPHTWNAGDVIDEQRGYRRGISWYRKKLFIPSEARDKKITLRFDGVASKADVYLNGKLLQTHLGAYTAFGVDITDICEVGKENLLAVKVDNSSSLGEILPSVSGDFSIFGGIYRRVFLQWTERVHFVTEPYAAVPVRIQTPEVSVSEASMQIVAFLKNDFTDTKHVHVNVFLCDEMNRIVKEKQLKLKLIPGRKYPISTSVGRIENPHLWSPELPYLYTVKVQVCDAKNGEMYQEVISPVGFRWFSVDKTGFYLNGKYLKLRGAARHQDYAGLGTAIPVEMNRRDMRLLKEMGANFVRISHYPQDPEIYRACDELGLIVWSEICVVNEVRKNTKFAHNCKEMLKEMILQNYNHPSVVLWGAMNELWDYHKQAIVLARELEALKKELDPYRLSCVAFHAFTWEKPYTQSSKEMFNISDVNGVNVYESWYQGDSATIAPMFDKFCSYSTAKPRFLSEFGAGSDERIHSYTPRTFDFTPEFQLDFNRRYINEMEKRPDYIGYSIWNLVDFQVDGRGDSKPNLNQKGMLTEDRRKKEIYYYCQARWSDIPMIHIAGADWTKRVEICDDSINVRKISVFSNQKTVELIHNGKSLGVREVVNGEAVFVVPFINGENLLDARSGALSDRLKIQMRLLSSRLTDSDVLLDGLCINLGQEHCYFIDPQLQEIWIPDKPYTKGSWGYMDGKPFNSWPGSSHDGVRYGVGADIKNTFLEPLFQTFLIGTTCYRLDVPDGVYEIGFYFTEPFSKDERKNIVRTGVSAEGQRVFDVSVNGEKLIESLNLADSYGEQTAVVKTLVVNVRNHEGLEILLSPQKGQGVISGLKVKKIR
TCTGAAATTTCTATAACAGATTCATGGAAGTATAAAGCAGAGAATGATGAACGTTTTTCCAGTATGGATTGGAATGATTCTGATTGGGTAACTGTAGATTTGCCCCATACTTGGAATGCCGGAGATGTGATTGACGAGCAACGGGGATATAGGCGCGGCATATCTTGGTATAGGAAGAAGTTGTTCATTCCTTCAGAGGCACGGGATAAGAAAATAACTCTTCGTTTTGATGGAGTGGCCAGCAAGGCAGACGTCTATTTAAATGGGAAATTATTGCAAACTCATTTGGGGGCATATACCGCTTTCGGAGTTGATATAACTGATATCTGTGAAGTTGGAAAGGAAAATCTGTTGGCGGTAAAAGTGGATAATAGTTCTTCTTTGGGGGAAATCCTTCCATCGGTTTCCGGAGATTTTTCTATTTTTGGGGGTATTTATCGCAGGGTGTTCTTGCAATGGACGGAAAGAGTGCATTTTGTGACAGAACCTTATGCAGCTGTTCCAGTAAGGATACAAACCCCTGAAGTGTCGGTATCTGAGGCGTCAATGCAAATTGTGGCTTTTCTTAAAAATGACTTTACCGATACAAAGCACGTGCACGTCAATGTTTTTCTTTGCGATGAAATGAATCGGATTGTGAAAGAGAAACAGTTGAAACTGAAACTGATTCCAGGCAGGAAATATCCTATATCCACTTCTGTAGGAAGGATAGAGAATCCTCATTTGTGGTCTCCTGAATTACCTTATTTATATACGGTAAAAGTGCAGGTATGCGATGCCAAGAATGGAGAAATGTATCAAGAAGTAATTTCTCCGGTTGGATTCAGATGGTTTTCGGTGGACAAGACAGGCTTTTATCTGAACGGGAAGTATCTTAAATTGAGGGGAGCTGCACGGCATCAGGATTATGCAGGTTTGGGAACGGCTATTCCCGTTGAAATGAATCGTAGGGATATGCGGTTGCTGAAAGAAATGGGAGCTAATTTTGTGCGTATCAGTCATTATCCTCAAGATCCGGAAATATATCGAGCCTGTGACGAATTGGGGTTGATAGTATGGTCTGAAATTTGTGTTGTGAATGAAGTGAGAAAAAATACGAAATTCGCTCATAACTGCAAAGAAATGTTGAAAGAGATGATTCTTCAGAACTACAATCATCCATCGGTGGTACTATGGGGGGCTATGAATGAATTATGGGATTACCATAAGCAAGCTATTGTGTTGGCTAGGGAATTGGAGGCTTTGAAAAAAGAGCTGGATCCTTATCGTCTTTCTTGTGTCGCCTTTCATGCTTTTACTTGGGAGAAACCCTATACACAGTCTAGTAAGGAGATGTTTAATATTTCGGATGTTAACGGGGTAAATGTATATGAATCTTGGTATCAGGGAGATTCTGCTACGATTGCACCTATGTTTGATAAATTTTGTAGTTATTCTACTGCAAAACCTCGTTTTTTGTCGGAATTTGGAGCAGGAAGCGATGAACGTATCCATTCTTATACTCCGCGTACATTTGATTTTACTCCGGAATTTCAATTGGATTTTAATCGCAGATATATCAATGAAATGGAGAAACGTCCGGATTATATAGGTTATTCTATTTGGAATTTGGTTGATTTTCAGGTGGACGGTCGTGGTGATAGCAAGCCTAATCTGAATCAAAAAGGAATGTTGACAGAGGATCGTCGGAAGAAAGAGATTTATTATTACTGTCAGGCTCGTTGGTCGGACATTCCGATGATTCATATTGCCGGGGCCGATTGGACGAAAAGGGTGGAAATTTGTGATGATAGTATAAATGTGCGTAAGATCAGTGTTTTTTCTAATCAGAAAACGGTGGAGTTAATTCATAATGGAAAGTCTTTGGGAGTGCGTGAAGTGGTAAATGGGGAAGCTGTTTTCGTAGTTCCTTTTATAAACGGAGAGAATCTGCTGGATGCCCGAAGTGGGGCACTTTCAGACAGGCTGAAGATACAGATGAGATTGTTATCTTCCCGTCTTACTGACAGTGATGTGCTTTTGGACGGTCTTTGTATTAATTTGGGACAGGAGCATTGTTATTTTATAGACCCGCAGCTTCAGGAAATATGGATACCCGATAAACCTTATACAAAGGGAAGTTGGGGATATATGGATGGAAAACCTTTTAACTCCTGGCCGGGATCATCACATGATGGAGTTAGGTATGGAGTAGGGGCTGATATAAAAAATACTTTTTTAGAGCCTCTTTTTCAGACCTTTTTAATTGGTACGACTTGTTACCGGCTGGATGTTCCTGACGGAGTATATGAAATTGGCTTTTATTTCACGGAACCTTTCAGTAAGGATGAACGGAAAAATATTGTGCGTACAGGTGTTTCTGCCGAGGGACAACGTGTATTTGATGTGTCAGTCAATGGAGAAAAATTGATAGAGTCATTGAATCTGGCTGATTCTTATGGCGAGCAGACAGCTGTTGTAAAGACACTTGTGGTCAATGTGCGGAATCATGAAGGACTGGAGATTCTGCTTTCTCCTCAAAAAGGACAAGGTGTTATTAGCGGATTAAAAGTAAAGAAAATACGA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,670 total letters Query= CMR200071.105 Length=2970 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4617 0.0 1_04D_D7_CMR200071.105_T7term.seq 1433 0.0 2_07D_D7_CMR200071.105_T7S.seq 1162 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 58 TTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAA-GGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTG 146 2 17 ....T...............................A..................................................... 106 W S H P Q F E K G T E N L Y F Q S N A S E I S I T D S W K Y Query_1 147 GTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCTCTGAAATTTCTATAACAGATTCATGGAAGTATAA 236 0 1 ................................... 35 2 107 .......................................................................................... 196 K A E N D E R F S S M D W N D S D W V T V D L P H T W N A G Query_1 237 AGCAGAGAATGATGAACGTTTTTCCAGTATGGATTGGAATGATTCTGATTGGGTAACTGTAGATTTGCCCCATACTTGGAATGCCGGAGA 326 0 36 .......................................................................................... 125 2 197 ........................................................................................N. 286 D V I D E Q R G Y R R G I S W Y R K K L F I P S E A R D K K Query_1 327 TGTGATTGACGAGCAACGGGGATATAGGCGCGGCATATCTTGGTATAGGAAGAAGTTGTTCATTCCTTCAGAGGCACGGGATAAGAAAAT 416 0 126 .......................................................................................... 215 2 287 ..........N............................................................................... 376 I T L R F D G V A S K A D V Y L N G K L L Q T H L G A Y T A Query_1 417 AACTCTTCGTTTTGATGGAGTGGCCAGCAAGGCAGACGTCTATTTAAATGGGAAATTATTGCAAACTCATTTGGGGGCATATACCGCTTT 506 0 216 .......................................................................................... 305 2 377 ................................NN........................................................ 466 F G V D I T D I C E V G K E N L L A V K V D N S S S L G E I Query_1 507 CGGAGTTGATATAACTGATATCTGTGAAGTTGGAAAGGAAAATCTGTTGGCGGTAAAAGTGGATAATAGTTCTTCTTTGGGGGAAATCCT 596 0 306 .......................................................................................... 395 2 467 ................................................................................N......... 556 L P S V S G D F S I F G G I Y R R V F L Q W T E R V H F V T Query_1 597 TCCATCGGTTTCCGGAGATTTTTCTATTTTTGGGGGTATTTATCGCAGGGTGTTCTTGCAATGGACGGAAAGAGTGCATTTTGTGACAGA 686 0 396 .......................................................................................... 485 2 557 ...............................................N.......................................... 646 E P Y A A V P V R I Q T P E V S V S E A S M Q I V A F L K N Query_1 687 ACCTTATGCAGCTGTTCCAGTAAGGATACAAACCCCTGAAGTGTCGGTATCTGAGGCGTCAATGCAAATTGTGGCTTTTCTTAAAAATGA 776 0 486 .......................................................................................... 575 2 647 ..........N........................ 681 D F T D T K H V H V N V F L C D E M N R I V K E K Q L K L K Query_1 777 CTTTACCGATACAAAGCACGTGCACGTCAATGTTTTTCTTTGCGATGAAATGAATCGGATTGTGAAAGAGAAACAGTTGAAACTGAAACT 866 0 576 .......................................................................................... 665 0 933 ..T............ 947 L I P G R K Y P I S T S V G R I E N P H L W S P E L P Y L Y Query_1 867 GATTCCAGGCAGGAAATATCCTATATCCACTTCTGTAGGAAGGATAGAGAATCCTCATTTGTGGTCTCCTGAATTACCTTATTTATATAC 956 0 666 .......................................................................................... 755 0 356 . 356 2 517 . 517 T V K V Q V C D A K N G E M Y Q E V I S P V G F R W F S V D Query_1 957 GGTAAAAGTGCAGGTATGCGATGCCAAGAATGGAGAAATGTATCAAGAAGTAATTTCTCCGGTTGGATTCAGATGGTTTTCGGTGGACAA 1046 0 756 .......................................................................................... 845 0 357 .......... 366 2 518 .......... 527 K T G F Y L N G K Y L K L R G A A R H Q D Y A G L G T A I P Query_1 1047 GACAGGCTTTTATCTGAACGGGAAGTATCTTAAATTGAGGGGAGCTGCACGGCATCAGGATTATGCAGGTTTGGGAACGGCTATTCCCGT 1136 0 846 .......................................................................................... 935 0 618 ..A 620 V E M N R R D M R L L K E M G A N F V R I S H Y P Q D P E I Query_1 1137 TGAAATGAATCGTAGGGATATGCGGTTGCTGAAAGAAATGGGAGCTAATTTTGTGCGTATCAGTCATTATCCTCAAGATCCGGAAATATA 1226 0 936 .......................................................................................... 1025 0 621 ............ 632 Y R A C D E L G L I V W S E I C V V N E V R K N T K F A H N Query_1 1227 TCGAGCCTGTGACGAATTGGGGTTGATAGTATGGTCTGAAATTTGTGTTGTGAATGAAGTGAGAAAAAATACGAAATTCGCTCATAACTG 1316 0 1026 .......................................................................................... 1115 C K E M L K E M I L Q N Y N H P S V V L W G A M N E L W D Y Query_1 1317 CAAAGAAATGTTGAAAGAGATGATTCTTCAGAACTACAATCATCCATCGGTGGTACTATGGGGGGCTATGAATGAATTATGGGATTACCA 1406 0 1116 .......................................................................................... 1205 H K Q A I V L A R E L E A L K K E L D P Y R L S C V A F H A Query_1 1407 TAAGCAAGCTATTGTGTTGGCTAGGGAATTGGAGGCTTTGAAAAAAGAGCTGGATCCTTATCGTCTTTCTTGTGTCGCCTTTCATGCTTT 1496 0 1206 .......................................................................................... 1295 F T W E K P Y T Q S S K E M F N I S D V N G V N V Y E S W Y Query_1 1497 TACTTGGGAGAAACCCTATACACAGTCTAGTAAGGAGATGTTTAATATTTCGGATGTTAACGGGGTAAATGTATATGAATCTTGGTATCA 1586 0 1296 .......................................................................................... 1385 Q G D S A T I A P M F D K F C S Y S T A K P R F L S A F G A Query_1 1587 GGGAGATTCTGCTACGATTGCACCTATGTTTGATAAATTTTGTAGTTATTCTACTGCAAAACCTCGTTTTTTGTCGGCTTTTGGAGCAGG 1676 0 1386 .............................................................................AA........... 1475 1 187 ............ 176 G S D E R I H S Y T P R T F D F T P E F Q L D F N R R Y I N Query_1 1677 AAGCGATGAACGTATCCATTCTTATACTCCGCGTACATTTGATTTTACTCCGGAATTTCAATTGGATTTTAATCGCAGATATATCAATGA 1766 0 1476 .......................................................................................... 1565 E M E K R P D Y I G Y S I W N L V D F Q V D G R G D S K P N Query_1 1767 AATGGAGAAACGTCCGGATTATATAGGTTATTCTATTTGGAATTTGGTTGATTTTCAGGTGGACGGTCGTGGTGATAGCAAGCCTAATCT 1856 0 1566 .......................................................................................... 1655 1 1074 ........ 1067 L N Q K G M L T E D R R K K E I Y Y Y C Q A R W S D I Query_1 1857 GAATCAAAAAGGAAT--GTTGACAGA-GGATCGTCGGAAGAAAGAG--ATTTATT-ATTACTG-TCAGG--CTCGTTG-GTCGGACATTC 1936 0 1656 ...............--.........-...................--.......-.......-.....--.......-........... 1735 1 1066 ............N.NTNN..AC.N..N..T.NNNN.N..N...N.AGA...N...N..N..N.G.....NN.C.....N..N..N....A 977 P M I H I A G A D W T K R V E I C D D S I N V R K I S Query_1 1937 CGATGATTCATATT-GCCGGGG--CCGATTGGACGA-AAAGGGTGG-AAATTTGTGAT-GATAG-TATAAA-TGTGC-GTAAGATC-AGT 2016 0 1736 ..............-.......--............-.........-...........-.....-......-.....-........-... 1815 1 976 ...A.NA.T..N.NN.......GC...N.G......N......N..G........NNNN.....N.N....ANN...N.A......CN.. 887 V F S N Q K T V E L I H N G K S L G V R E V V N G E A Query_1 2017 GTTTTTTCTAATCAGAAAA-CGGTGG--AGTTAATTCATAATGG-AAAGTCTTTGGGA-GTGCGTGAA-GTGG-TAAA-TGGGGAAG-CT 2097 0 1816 ...................-......--................-.............-.........-....-....-........-.. 1896 1 886 .........N...N.....N...NN.GN.....T..........N.............A.........A....G....N........G.. 797 V F V V P F I N G E N L L D A R S G A L S D R L K I Q M Query_1 2098 G-TTTTCGTAGTTCCTTTTATAAA-CGGAGAGAATC-TGCT-GGATGCCCGAAGTGGGGCACTTTCAGACAGGCTGAAGATACAGA-TGA 2182 0 1897 .-......................-...........-....-............................................-... 1981 1 796 .N......................A..........NN.N..T.N.N............................N...........A..N 707 R L L S S R L T D S D V L L D G L C I N L G Q E H C Y F I Query_1 2183 GATTGTTATCTTCCCGTCTTACTG-ACAGTGATGTGCTTTTGGACGGTCTT-TGTATTAATTTGGGACAGGAGCATTGTTATTTTATAGA 2270 0 1982 ........................-..........................-...................................... 2069 1 706 ........................G.....................N....N.......................N.............. 617 D P Q L Q E I W I P D K P Y T K G S W G Y M D G K P F N S W Query_1 2271 CCCGCAGCTTCAGGAAATATGGATACCCGATAAACCTTATACAAAGGGAAGTTGGGGATATATGGATGGAAAACCTTTTAACTCCTGGCC 2360 0 2070 .......................................................................................... 2159 1 616 .......................................................................................... 527 P G S S H D G V R Y G V G A D I K N T F L E P L F Q T F L I Query_1 2361 GGGATCATCACATGATGGAGTTAGGTATGGAGTAGGGGCTGATATAAAAAATACTTTTTTAGAGCCTCTTTTTCAGACCTTTTTAATTGG 2450 0 2160 .......................................................................................... 2249 1 526 .......................................................................................... 437 G T T C Y R L D V P D G V Y E I G F Y F T E P F S K D E R K Query_1 2451 TACGACTTGTTACCGGCTGGATGTTCCTGACGGAGTATATGAAATTGGCTTTTATTTCACGGAACCTTTCAGTAAGGATGAACGGAAAAA 2540 0 2250 .......................................................................................... 2339 1 436 .......................................................................................... 347 N I V R T G V S A E G Q R V F D V S V N G E K L I E S L N L Query_1 2541 TATTGTGCGTACAGGTGTTTCTGCCGAGGGACAACGTGTATTTGATGTGTCAGTCAATGGAGAAAAATTGATAGAGTCATTGAATCTGGC 2630 0 2340 .......................................................................................... 2429 1 346 .......................................................................................... 257 A D S Y G E Q T A V V K T L V V N V R N H E G L E I L L S P Query_1 2631 TGATTCTTATGGCGAGCAGACAGCTGTTGTAAAGACACTTGTGGTCAATGTGCGGAATCATGAAGGACTGGAGATTCTGCTTTCTCCTCA 2720 0 2430 .......................................................................................... 2519 1 256 .......................................................................................... 167 Q K G Q G V I S G L K V K K I R * H W K W I T D P N S S S V Query_1 2721 AAAAGGACAAGGTGTTATTAGCGGATTAAAAGTAAAGAAAATACGATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGA 2810 0 2520 .............................................. 2565 1 166 .......................................................................................... 77 D K L A A A L E H H H H H H * D P A A N K A R K Query_1 2811 CAAGCTTGCGGCCGCACTCGAGcaccaccaccaccacca-cTGAGAT-CCGGCTGCTAACAAAGCCCGAAAG 2880 1 76 ......AA...N...........................T.......C........................ 5 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 13639907 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,670 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200071.106 (CMR200071.21-875.P(875).E417Q.pMCSG68)
SEISITDSWKYKAENDERFSSMDWNDSDWVTVDLPHTWNAGDVIDEQRGYRRGISWYRKKLFIPSEARDKKITLRFDGVASKADVYLNGKLLQTHLGAYTAFGVDITDICEVGKENLLAVKVDNSSSLGEILPSVSGDFSIFGGIYRRVFLQWTERVHFVTEPYAAVPVRIQTPEVSVSEASMQIVAFLKNDFTDTKHVHVNVFLCDEMNRIVKEKQLKLKLIPGRKYPISTSVGRIENPHLWSPELPYLYTVKVQVCDAKNGEMYQEVISPVGFRWFSVDKTGFYLNGKYLKLRGAARHQDYAGLGTAIPVEMNRRDMRLLKEMGANFVRISHYPQDPEIYRACDELGLIVWSEICVVNEVRKNTKFAHNCKEMLKEMILQNYNHPSVVLWGAMNELWDYHKQAIVLARELEALKKELDPYRLSCVAFHAFTWEKPYTQSSKEMFNISDVNGVNVYESWYQGDSATIAPMFDKFCSYSTAKPRFLSEFGAGSDERIHSYTPRTFDFTPEFQLDFNRRYINEMEKRPDYIGYSIWNLVDFQVDGRGDSKPNLNQKGMLTEDRRKKEIYYYCQARWSDIPMIHIAGADWTKRVEICDDSINVRKISVFSNQKTVELIHNGKSLGVREVVNGEAVFVVPFINGENLLDARSGALSDRLKIQMRLLSSRLTDSDVLLDGLCINLGQEHCYFIDPQLQEIWIPDKPYTKGSWGYMDGKPFNSWPGSSHDGVRYGVGADIKNTFLEPLFQTFLIGTTCYRLDVPDGVYEIGFYFTEPFSKDERKNIVRTGVSAEGQRVFDVSVNGEKLIESLNLADSYGEQTAVVKTLVVNVRNHEGLEILLSPQKGQGVISGLKVKKIR
TCTGAAATTTCTATAACAGATTCATGGAAGTATAAAGCAGAGAATGATGAACGTTTTTCCAGTATGGATTGGAATGATTCTGATTGGGTAACTGTAGATTTGCCCCATACTTGGAATGCCGGAGATGTGATTGACGAGCAACGGGGATATAGGCGCGGCATATCTTGGTATAGGAAGAAGTTGTTCATTCCTTCAGAGGCACGGGATAAGAAAATAACTCTTCGTTTTGATGGAGTGGCCAGCAAGGCAGACGTCTATTTAAATGGGAAATTATTGCAAACTCATTTGGGGGCATATACCGCTTTCGGAGTTGATATAACTGATATCTGTGAAGTTGGAAAGGAAAATCTGTTGGCGGTAAAAGTGGATAATAGTTCTTCTTTGGGGGAAATCCTTCCATCGGTTTCCGGAGATTTTTCTATTTTTGGGGGTATTTATCGCAGGGTGTTCTTGCAATGGACGGAAAGAGTGCATTTTGTGACAGAACCTTATGCAGCTGTTCCAGTAAGGATACAAACCCCTGAAGTGTCGGTATCTGAGGCGTCAATGCAAATTGTGGCTTTTCTTAAAAATGACTTTACCGATACAAAGCACGTGCACGTCAATGTTTTTCTTTGCGATGAAATGAATCGGATTGTGAAAGAGAAACAGTTGAAACTGAAACTGATTCCAGGCAGGAAATATCCTATATCCACTTCTGTAGGAAGGATAGAGAATCCTCATTTGTGGTCTCCTGAATTACCTTATTTATATACGGTAAAAGTGCAGGTATGCGATGCCAAGAATGGAGAAATGTATCAAGAAGTAATTTCTCCGGTTGGATTCAGATGGTTTTCGGTGGACAAGACAGGCTTTTATCTGAACGGGAAGTATCTTAAATTGAGGGGAGCTGCACGGCATCAGGATTATGCAGGTTTGGGAACGGCTATTCCCGTTGAAATGAATCGTAGGGATATGCGGTTGCTGAAAGAAATGGGAGCTAATTTTGTGCGTATCAGTCATTATCCTCAAGATCCGGAAATATATCGAGCCTGTGACGAATTGGGGTTGATAGTATGGTCTGAAATTTGTGTTGTGAATGAAGTGAGAAAAAATACGAAATTCGCTCATAACTGCAAAGAAATGTTGAAAGAGATGATTCTTCAGAACTACAATCATCCATCGGTGGTACTATGGGGGGCTATGAATGAATTATGGGATTACCATAAGCAAGCTATTGTGTTGGCTAGGGAATTGGAGGCTTTGAAAAAAGAGCTGGATCCTTATCGTCTTTCTTGTGTCGCCTTTCATGCTTTTACTTGGGAGAAACCCTATACACAGTCTAGTAAGGAGATGTTTAATATTTCGGATGTTAACGGGGTAAATGTATATGAATCTTGGTATCAGGGAGATTCTGCTACGATTGCACCTATGTTTGATAAATTTTGTAGTTATTCTACTGCAAAACCTCGTTTTTTGTCGGAATTTGGAGCAGGAAGCGATGAACGTATCCATTCTTATACTCCGCGTACATTTGATTTTACTCCGGAATTTCAATTGGATTTTAATCGCAGATATATCAATGAAATGGAGAAACGTCCGGATTATATAGGTTATTCTATTTGGAATTTGGTTGATTTTCAGGTGGACGGTCGTGGTGATAGCAAGCCTAATCTGAATCAAAAAGGAATGTTGACAGAGGATCGTCGGAAGAAAGAGATTTATTATTACTGTCAGGCTCGTTGGTCGGACATTCCGATGATTCATATTGCCGGGGCCGATTGGACGAAAAGGGTGGAAATTTGTGATGATAGTATAAATGTGCGTAAGATCAGTGTTTTTTCTAATCAGAAAACGGTGGAGTTAATTCATAATGGAAAGTCTTTGGGAGTGCGTGAAGTGGTAAATGGGGAAGCTGTTTTCGTAGTTCCTTTTATAAACGGAGAGAATCTGCTGGATGCCCGAAGTGGGGCACTTTCAGACAGGCTGAAGATACAGATGAGATTGTTATCTTCCCGTCTTACTGACAGTGATGTGCTTTTGGACGGTCTTTGTATTAATTTGGGACAGGAGCATTGTTATTTTATAGACCCGCAGCTTCAGGAAATATGGATACCCGATAAACCTTATACAAAGGGAAGTTGGGGATATATGGATGGAAAACCTTTTAACTCCTGGCCGGGATCATCACATGATGGAGTTAGGTATGGAGTAGGGGCTGATATAAAAAATACTTTTTTAGAGCCTCTTTTTCAGACCTTTTTAATTGGTACGACTTGTTACCGGCTGGATGTTCCTGACGGAGTATATGAAATTGGCTTTTATTTCACGGAACCTTTCAGTAAGGATGAACGGAAAAATATTGTGCGTACAGGTGTTTCTGCCGAGGGACAACGTGTATTTGATGTGTCAGTCAATGGAGAAAAATTGATAGAGTCATTGAATCTGGCTGATTCTTATGGCGAGCAGACAGCTGTTGTAAAGACACTTGTGGTCAATGTGCGGAATCATGAAGGACTGGAGATTCTGCTTTCTCCTCAAAAAGGACAAGGTGTTATTAGCGGATTAAAAGTAAAGAAAATACGA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,010 total letters Query= CMR200071.106 Length=2970 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4617 0.0 2_07E_E7_CMR200071.106_T7S.seq 1909 0.0 1_04E_E7_CMR200071.106_T7term.seq 1835 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 57 ATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTG 146 2 16 .......................................................................................... 105 W S H P Q F E K G T E N L Y F Q S N A S E I S I T D S W K Y Query_1 147 GTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCTCTGAAATTTCTATAACAGATTCATGGAAGTATAA 236 0 1 ................................... 35 2 106 .......................................................................................... 195 K A E N D E R F S S M D W N D S D W V T V D L P H T W N A G Query_1 237 AGCAGAGAATGATGAACGTTTTTCCAGTATGGATTGGAATGATTCTGATTGGGTAACTGTAGATTTGCCCCATACTTGGAATGCCGGAGA 326 0 36 .......................................................................................... 125 2 196 .......................................................................................... 285 D V I D E Q R G Y R R G I S W Y R K K L F I P S E A R D K K Query_1 327 TGTGATTGACGAGCAACGGGGATATAGGCGCGGCATATCTTGGTATAGGAAGAAGTTGTTCATTCCTTCAGAGGCACGGGATAAGAAAAT 416 0 126 .......................................................................................... 215 2 286 .......................................................................................... 375 I T L R F D G V A S K A D V Y L N G K L L Q T H L G A Y T A Query_1 417 AACTCTTCGTTTTGATGGAGTGGCCAGCAAGGCAGACGTCTATTTAAATGGGAAATTATTGCAAACTCATTTGGGGGCATATACCGCTTT 506 0 216 .......................................................................................... 305 2 376 .......................................................................................... 465 F G V D I T D I C E V G K E N L L A V K V D N S S S L G E I Query_1 507 CGGAGTTGATATAACTGATATCTGTGAAGTTGGAAAGGAAAATCTGTTGGCGGTAAAAGTGGATAATAGTTCTTCTTTGGGGGAAATCCT 596 0 306 .......................................................................................... 395 2 466 .......................................................................................... 555 2 917 ........... 927 L P S V S G D F S I F G G I Y R R V F L Q W T E R V H F V T Query_1 597 TCCATCGGTTTCCGGAGATTTTTCTATTTTTGGGGGTATTTATCGCAGGGTGTTCTTGCAATGGACGGAAAGAGTGCATTTTGTGACAGA 686 0 396 .......................................................................................... 485 2 556 .......................................................................................... 645 E P Y A A V P V R I Q T P E V S V S E A S M Q I V A F L K N Query_1 687 ACCTTATGCAGCTG-TTCCAGTAAGGATACAAACCCCTGAAGTGTCGGTATCTGAGGCGTCAATGCAAATTGTGGCTTTTCTTAAAAATG 775 0 486 ..............-........................................................................... 574 2 646 ..............T........................................................................... 735 D F T D T K H V H V N V F L C D E M N R I V K E K Q L K L K Query_1 776 ACTTTACCGATACAAAGCACGTGCACGTCAATGTTTTTCTTTGCGATGAAATGAATCGGATTGTGAAAGAGAAACAGTTGAAACTG-AAA 864 0 575 ......................................................................................-... 663 0 933 ..T............ 947 2 736 ......................................................................................A... 825 L I P G R K Y P I S T S V G R I E N P H L W S P E L P Y L Y Query_1 865 CTGATTCCAGGCAGGAAATATCCTATATCCACTTCTGTAGGAAGGATAGAGAATCCTCATTTGTGGTCTCCTGAATTACCTTATTTATAT 954 0 664 .......................................................................................... 753 2 826 .......................................................................................... 915 T V K V Q V C D A K N G E M Y Q E V I S P V G F R W F S V Query_1 955 ACGGTAAAAGTGCAGGTATGCGATGCCAAGAATGGAGAAATGTATCAAGAAGTAATTTC-TCCGGTTGGATTCAGATGGTTTTCGGTGGA 1043 0 754 ...........................................................-.............................. 842 0 356 ........... 366 2 916 ....................................N......................T...................-.......... 1004 2 516 ........... 526 D K T G F Y L N G K Y L K L R G A A R H Q D Y A G L G T A Query_1 1044 CAAGACAGGC-TTTTATCTGAACGGGAAGTATCTTAAATTGA-GGGGAGCTGCACGGCATCAGG-ATTATGCAGGTTTGGGAACGGCTAT 1130 0 843 ..........-...............................-.....................-......................... 929 2 1005 ..........T..................NN...........G..........N.......N..A..A............CN......TA 1094 I P V E M N R R D M R L L K E M G A N F V R I S H Y P Q D Query_1 1131 TCCCGTTGAAATGAATCGTAGGGATATGCGGTTGCTGAAAGAAATGGGAGCTAA-TTTTGTGCG-TATCAG-TCATTATCCTCAAGATCC 1217 0 930 ......................................................-.........-......-.................. 1016 0 618 ..A............ 632 2 1095 .T.N........-NN........--.NNNCNNN...TG.N.-.......N....T.........N......T............GA.... 1180 2 779 ..A............ 793 P E I Y R A C D E L G L I V W S E I C V V N E V R K N T K F Query_1 1218 GGAAATATATCGAGCCTGTGACGAATTGGGGTTGATAGTATGGTCTGAAATTTGTGTTGTGAATGAAGTGAGAAAAAATACGAAATTCGC 1307 0 1017 .......................................................................................... 1106 2 1181 ..N....... 1190 A H N C K E M L K E M I L Q N Y N H P S V V L W G A M N Q L Query_1 1308 TCATAACTGCAAAGAAATGTTGAAAGAGATGATTCTTCAGAACTACAATCATCCATCGGTGGTACTATGGGGGGCTATGAATCAGTTATG 1397 0 1107 ..................................................................................G.A..... 1196 W D Y H K Q A I V L A R E L E A L K K E L D P Y R L S C V A Query_1 1398 GGATTACCATAAGCAAGCTATTGTGTTGGCTAGGGAATTGGAGGCTTTGAAAAAAGAGCTGGATCCTTATCGTCTTTCTTGTGTCGCCTT 1487 0 1197 .......................................................................................... 1286 F H A F T W E K P Y T Q S S K E M F N I S D V N G V N V Y E Query_1 1488 TCATGCTTTTACTTGGGAGAAACCCTATACACAGTCTAGTAAGGAGATGTTTAATATTTCGGATGTTAACGGGGTAAATGTATATGAATC 1577 0 1287 .......................................................................................... 1376 S W Y Q G D S A T I A P M F D K F C S Y S T A K P R F L S E Query_1 1578 TTGGTATCAGGGAGATTCTGCTACGATTGCACCTATGTTTGATAAATTTTGTAGTTATTCTACTGCAAAACCTCGTTTTTTGTCGGAATT 1667 0 1377 .......................................................................................... 1466 1 200 ............ 189 F G A G S D E R I H S Y T P R T F D F T P E F Q L D F N R R Query_1 1668 TGGAGCAGGAAGCGATGAACGTATCCATTCTTATACTCCGCGTACATTTGATTTTACTCCGGAATTTCAATTGGATTTTAATCGCAGATA 1757 0 1467 .......................................................................................... 1556 1 1146 ... 1144 Y I N E M E K R P D Y I G Y S I W N L V D F Q V D G R G D Query_1 1758 TATCAATGAAATGG-AGAAAC-GTCCGGATTATATAGGTTATTCTATTTGGAATTTGGTTGATTTTCAGGTGGACGGTCG-TGGTGATAG 1844 0 1557 ..............-......-..........................................................-......... 1643 1 1143 .....N........N......C........NNNN...N.-...NN....N..-...................-.......G......... 1057 S K P N L N Q K G M L T E D R R K K E I Y Y Y C Q A R W S Query_1 1845 CAAGCCT-AATCTGAATCAAAAAGGAATGTTGACAGAGGATCG-TCGGAAGAAA-GAGATTTATTATTACTGTCAGGCTCGTTGGTCGGA 1931 0 1644 .......-...................................-..........-................................... 1730 1 1056 .......T.........NC...........-............G..........A............-........-............. 970 D I P M I H I A G A D W T K R V E I C D D S I N V R K I S V Query_1 1932 CATTCCGATGATTCATATTGCCGGGGCCGATT-GGACGAAAAGGGTGGAAATTTGTGATG-ATAGTATAAATGTGCGTAAGATCAGTGTT 2019 0 1731 ................................-...........................-............................. 1818 1 969 .....GN..................-....N.N..................A........N.......N..................... 881 F S N Q K T V E L I H N G K S L G V R E V V N G E A V F V Query_1 2020 TTTTCTAATCAGAAAACGGTGGAGTTAATTCA-TAATGGAAAGTCTTTGGGAGTGCGTGAAGTGGTAAATGGGGAAGCTGTTTTCGTAGT 2108 0 1819 ................................-......................................................... 1907 1 880 ...............-................N..................................................-...... 793 V P F I N G E N L L D A R S G A L S D R L K I Q M R L L S S Query_1 2109 TCCTTTTATAAACGGAGAGAATCTGCTGGATGCCCGAAGTGGGGCACTTTCAGACAGGCTGAA-GATACAGATGAGATTGTTATCTTCCC 2197 0 1908 ...............................................................-.......................... 1996 1 792 ...............................................................A.......................... 703 R L T D S D V L L D G L C I N L G Q E H C Y F I D P Q L Q E Query_1 2198 GTCTTACTGACAGTGATGTGCTTTTGGACGGTCTTTGTATTAATTTGGGACAGGAGCATTGTTATTTTATAGACCCGCAGCTTCAGGAAA 2287 0 1997 .......................................................................................... 2086 1 702 .......................................................................................... 613 I W I P D K P Y T K G S W G Y M D G K P F N S W P G S S H D Query_1 2288 TATGGATACCCGATAAACCTTATACAAAGGGAAGTTGGGGATATATGGATGGAAAACCTTTTAACTCCTGGCCGGGATCATCACATGATG 2377 0 2087 .......................................................................................... 2176 1 612 .......................................................................................... 523 G V R Y G V G A D I K N T F L E P L F Q T F L I G T T C Y R Query_1 2378 GAGTTAGGTATGGAGTAGGGGCTGATATAAAAAATACTTTTTTAGAGCCTCTTTTTCAGACCTTTTTAATTGGTACGACTTGTTACCGGC 2467 0 2177 .......................................................................................... 2266 1 522 .......................................................................................... 433 L D V P D G V Y E I G F Y F T E P F S K D E R K N I V R T G Query_1 2468 TGGATGTTCCTGACGGAGTATATGAAATTGGCTTTTATTTCACGGAACCTTTCAGTAAGGATGAACGGAAAAATATTGTGCGTACAGGTG 2557 0 2267 .......................................................................................... 2356 1 432 .......................................................................................... 343 V S A E G Q R V F D V S V N G E K L I E S L N L A D S Y G E Query_1 2558 TTTCTGCCGAGGGACAACGTGTATTTGATGTGTCAGTCAATGGAGAAAAATTGATAGAGTCATTGAATCTGGCTGATTCTTATGGCGAGC 2647 0 2357 .......................................................................................... 2446 1 342 .......................................................................................... 253 Q T A V V K T L V V N V R N H E G L E I L L S P Q K G Q G V Query_1 2648 AGACAGCTGTTGTAAAGACACTTGTGGTCAATGTGCGGAATCATGAAGGACTGGAGATTCTGCTTTCTCCTCAAAAAGGACAAGGTGTTA 2737 0 2447 .......................................................................................... 2536 1 252 .......................................................................................... 163 I S G L K V K K I R * H W K W I T D P N S S S V D K L A A A Query_1 2738 TTAGCGGATTAAAAGTAAAGAAAATACGATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCAC 2827 0 2537 ............................. 2565 1 162 .......................................................................................... 73 L E H H H H H H * D P A A N K A R K E Query_1 2828 TCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAA 2883 1 72 ....................................................A... 17 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 14643927 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 5,010 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200071.107 (CMR200071.21-875.P(875).E508Q.pMCSG68)
SEISITDSWKYKAENDERFSSMDWNDSDWVTVDLPHTWNAGDVIDEQRGYRRGISWYRKKLFIPSEARDKKITLRFDGVASKADVYLNGKLLQTHLGAYTAFGVDITDICEVGKENLLAVKVDNSSSLGEILPSVSGDFSIFGGIYRRVFLQWTERVHFVTEPYAAVPVRIQTPEVSVSEASMQIVAFLKNDFTDTKHVHVNVFLCDEMNRIVKEKQLKLKLIPGRKYPISTSVGRIENPHLWSPELPYLYTVKVQVCDAKNGEMYQEVISPVGFRWFSVDKTGFYLNGKYLKLRGAARHQDYAGLGTAIPVEMNRRDMRLLKEMGANFVRISHYPQDPEIYRACDELGLIVWSEICVVNEVRKNTKFAHNCKEMLKEMILQNYNHPSVVLWGAMNELWDYHKQAIVLARELEALKKELDPYRLSCVAFHAFTWEKPYTQSSKEMFNISDVNGVNVYESWYQGDSATIAPMFDKFCSYSTAKPRFLSEFGAGSDERIHSYTPRTFDFTPEFQLDFNRRYINEMEKRPDYIGYSIWNLVDFQVDGRGDSKPNLNQKGMLTEDRRKKEIYYYCQARWSDIPMIHIAGADWTKRVEICDDSINVRKISVFSNQKTVELIHNGKSLGVREVVNGEAVFVVPFINGENLLDARSGALSDRLKIQMRLLSSRLTDSDVLLDGLCINLGQEHCYFIDPQLQEIWIPDKPYTKGSWGYMDGKPFNSWPGSSHDGVRYGVGADIKNTFLEPLFQTFLIGTTCYRLDVPDGVYEIGFYFTEPFSKDERKNIVRTGVSAEGQRVFDVSVNGEKLIESLNLADSYGEQTAVVKTLVVNVRNHEGLEILLSPQKGQGVISGLKVKKIR
TCTGAAATTTCTATAACAGATTCATGGAAGTATAAAGCAGAGAATGATGAACGTTTTTCCAGTATGGATTGGAATGATTCTGATTGGGTAACTGTAGATTTGCCCCATACTTGGAATGCCGGAGATGTGATTGACGAGCAACGGGGATATAGGCGCGGCATATCTTGGTATAGGAAGAAGTTGTTCATTCCTTCAGAGGCACGGGATAAGAAAATAACTCTTCGTTTTGATGGAGTGGCCAGCAAGGCAGACGTCTATTTAAATGGGAAATTATTGCAAACTCATTTGGGGGCATATACCGCTTTCGGAGTTGATATAACTGATATCTGTGAAGTTGGAAAGGAAAATCTGTTGGCGGTAAAAGTGGATAATAGTTCTTCTTTGGGGGAAATCCTTCCATCGGTTTCCGGAGATTTTTCTATTTTTGGGGGTATTTATCGCAGGGTGTTCTTGCAATGGACGGAAAGAGTGCATTTTGTGACAGAACCTTATGCAGCTGTTCCAGTAAGGATACAAACCCCTGAAGTGTCGGTATCTGAGGCGTCAATGCAAATTGTGGCTTTTCTTAAAAATGACTTTACCGATACAAAGCACGTGCACGTCAATGTTTTTCTTTGCGATGAAATGAATCGGATTGTGAAAGAGAAACAGTTGAAACTGAAACTGATTCCAGGCAGGAAATATCCTATATCCACTTCTGTAGGAAGGATAGAGAATCCTCATTTGTGGTCTCCTGAATTACCTTATTTATATACGGTAAAAGTGCAGGTATGCGATGCCAAGAATGGAGAAATGTATCAAGAAGTAATTTCTCCGGTTGGATTCAGATGGTTTTCGGTGGACAAGACAGGCTTTTATCTGAACGGGAAGTATCTTAAATTGAGGGGAGCTGCACGGCATCAGGATTATGCAGGTTTGGGAACGGCTATTCCCGTTGAAATGAATCGTAGGGATATGCGGTTGCTGAAAGAAATGGGAGCTAATTTTGTGCGTATCAGTCATTATCCTCAAGATCCGGAAATATATCGAGCCTGTGACGAATTGGGGTTGATAGTATGGTCTGAAATTTGTGTTGTGAATGAAGTGAGAAAAAATACGAAATTCGCTCATAACTGCAAAGAAATGTTGAAAGAGATGATTCTTCAGAACTACAATCATCCATCGGTGGTACTATGGGGGGCTATGAATGAATTATGGGATTACCATAAGCAAGCTATTGTGTTGGCTAGGGAATTGGAGGCTTTGAAAAAAGAGCTGGATCCTTATCGTCTTTCTTGTGTCGCCTTTCATGCTTTTACTTGGGAGAAACCCTATACACAGTCTAGTAAGGAGATGTTTAATATTTCGGATGTTAACGGGGTAAATGTATATGAATCTTGGTATCAGGGAGATTCTGCTACGATTGCACCTATGTTTGATAAATTTTGTAGTTATTCTACTGCAAAACCTCGTTTTTTGTCGGAATTTGGAGCAGGAAGCGATGAACGTATCCATTCTTATACTCCGCGTACATTTGATTTTACTCCGGAATTTCAATTGGATTTTAATCGCAGATATATCAATGAAATGGAGAAACGTCCGGATTATATAGGTTATTCTATTTGGAATTTGGTTGATTTTCAGGTGGACGGTCGTGGTGATAGCAAGCCTAATCTGAATCAAAAAGGAATGTTGACAGAGGATCGTCGGAAGAAAGAGATTTATTATTACTGTCAGGCTCGTTGGTCGGACATTCCGATGATTCATATTGCCGGGGCCGATTGGACGAAAAGGGTGGAAATTTGTGATGATAGTATAAATGTGCGTAAGATCAGTGTTTTTTCTAATCAGAAAACGGTGGAGTTAATTCATAATGGAAAGTCTTTGGGAGTGCGTGAAGTGGTAAATGGGGAAGCTGTTTTCGTAGTTCCTTTTATAAACGGAGAGAATCTGCTGGATGCCCGAAGTGGGGCACTTTCAGACAGGCTGAAGATACAGATGAGATTGTTATCTTCCCGTCTTACTGACAGTGATGTGCTTTTGGACGGTCTTTGTATTAATTTGGGACAGGAGCATTGTTATTTTATAGACCCGCAGCTTCAGGAAATATGGATACCCGATAAACCTTATACAAAGGGAAGTTGGGGATATATGGATGGAAAACCTTTTAACTCCTGGCCGGGATCATCACATGATGGAGTTAGGTATGGAGTAGGGGCTGATATAAAAAATACTTTTTTAGAGCCTCTTTTTCAGACCTTTTTAATTGGTACGACTTGTTACCGGCTGGATGTTCCTGACGGAGTATATGAAATTGGCTTTTATTTCACGGAACCTTTCAGTAAGGATGAACGGAAAAATATTGTGCGTACAGGTGTTTCTGCCGAGGGACAACGTGTATTTGATGTGTCAGTCAATGGAGAAAAATTGATAGAGTCATTGAATCTGGCTGATTCTTATGGCGAGCAGACAGCTGTTGTAAAGACACTTGTGGTCAATGTGCGGAATCATGAAGGACTGGAGATTCTGCTTTCTCCTCAAAAAGGACAAGGTGTTATTAGCGGATTAAAAGTAAAGAAAATACGA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,953 total letters Query= CMR200071.107 Length=2970 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4617 0.0 2_07F_F7_CMR200071.107_T7S.seq 1561 0.0 1_04F_F7_CMR200071.107_T7term.seq 1483 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 60 CCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTC 149 2 22 ...........N-............................................................................. 110 S H P Q F E K G T E N L Y F Q S N A S E I S I T D S W K Y K Query_1 150 TCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCTCTGAAATTTCTATAACAGATTCATGGAAGTATAAAGC 239 0 1 ...................................... 38 2 111 .......................................................................................... 200 A E N D E R F S S M D W N D S D W V T V D L P H T W N A G D Query_1 240 AGAGAATGATGAACGTTTTTCCAGTATGGATTGGAATGATTCTGATTGGGTAACTGTAGATTTGCCCCATACTTGGAATGCCGGAGATGT 329 0 39 .......................................................................................... 128 2 201 .......................................................................................... 290 V I D E Q R G Y R R G I S W Y R K K L F I P S E A R D K K I Query_1 330 GATTGACGAGCAACGGGGATATAGGCGCGGCATATCTTGGTATAGGAAGAAGTTGTTCATTCCTTCAGAGGCACGGGATAAGAAAATAAC 419 0 129 .......................................................................................... 218 2 291 .......................................................................................... 380 T L R F D G V A S K A D V Y L N G K L L Q T H L G A Y T A F Query_1 420 TCTTCGTTTTGATGGAGTGGCCAGCAAGGCAGACGTCTATTTAAATGGGAAATTATTGCAAACTCATTTGGGGGCATATACCGCTTTCGG 509 0 219 .......................................................................................... 308 2 381 .......................................................................................... 470 G V D I T D I C E V G K E N L L A V K V D N S S S L G E I L Query_1 510 AGTTGATATAACTGATATCTGTGAAGTTGGAAAGGAAAATCTGTTGGCGGTAAAAGTGGATAATAGTTCTTCTTTGGGGGAAATCCTTCC 599 0 309 .......................................................................................... 398 2 471 .......................................................................................... 560 P S V S G D F S I F G G I Y R R V F L Q W T E R V H F V T E Query_1 600 ATCGGTTTCCGGAGATTTTTCTATTTTTGGGGGTATTTATCGCAGGGTGTTCTTGCAATGGACGGAAAGAGTGCATTTTGTGACAGAACC 689 0 399 .......................................................................................... 488 2 561 ........................................................................................T. 650 P Y A A V P V R I Q T P E V S V S E A S M Q I V A F L K N D Query_1 690 TTATGCAGCTGTTCCAGTAAGGATACAAACCCCTGAAGTGTCGGTATCTGAGGCGTCAATGCAAATTGTGGCTTTT--CTTAAAAATGAC 777 0 489 ............................................................................--............ 576 2 651 ............................................................................TCT........... 740 F T D T K H V H V N V F L C D E M N R I V K E K Q L K L K Query_1 778 TTTACCGATACAAAGCACGTGCACGTCAATGTTTTTC-TTTGCGATGAAATGAATCGGATTGTGAAAGAGAAACAGTTGAAACTGAAACT 866 0 577 .....................................-.................................................... 665 0 933 ..T............ 947 2 741 .....................................T.................................................... 830 L I P G R K Y P I S T S V G R I E N P H L W S P E L P Y L Query_1 867 GATTCCAGGCAGGAAATATCC-TATATCCACTTCTGTAGGAA-GGATAGAGAATCCT-CATTTGTGGTCTCC--TGAATTACCTTATTTA 951 0 666 .....................-....................-..............-..............--................ 750 2 831 ...........N.........T.......N............G.....N.NN.N..NC.N............CTG....N.......... 920 Y T V K V Q V C D A K N G E M Y Q E V I S P V G F R W F S Query_1 952 TATACGGTAAAA-GTGCAGGTATGCGAT-GCCAAGAAT-GGAGAAATGTATCAAGAAGTAATTTCTCCGGTTGGATTCAGATGGTTTTCG 1038 0 751 ............-...............-.........-................................................... 837 0 356 ........-... 366 2 921 ............N........N.....NG.........N...N..... 968 2 518 ........-... 528 V D K T G F Y L N G K Y L K L R G A A R H Q D Y A G L G T A Query_1 1039 GTGGACAAGACAGGCTTTTATCTGAACGGGAAGTATCTTAAATTGAGGGGAGCTGCACGGCATCAGGATTATGCAGGTTTGGGAACGGCT 1128 0 838 .......................................................................................... 927 I P V E M N R R D M R L L K E M G A N F V R I S H Y P Q D P Query_1 1129 ATTCCCGTTGAAATGAATCGTAGGGATATGCGGTTGCTGAAAGAAATGGGAGCTAATTTTGTGCGTATCAGTCATTATCCTCAAGATCCG 1218 0 928 .......................................................................................... 1017 0 618 ..A............ 632 2 783 ..A............ 797 E I Y R A C D E L G L I V W S E I C V V N E V R K N T K F A Query_1 1219 GAAATATATCGAGCCTGTGACGAATTGGGGTTGATAGTATGGTCTGAAATTTGTGTTGTGAATGAAGTGAGAAAAAATACGAAATTCGCT 1308 0 1018 .......................................................................................... 1107 H N C K E M L K E M I L Q N Y N H P S V V L W G A M N E L W Query_1 1309 CATAACTGCAAAGAAATGTTGAAAGAGATGATTCTTCAGAACTACAATCATCCATCGGTGGTACTATGGGGGGCTATGAATGAATTATGG 1398 0 1108 .......................................................................................... 1197 D Y H K Q A I V L A R E L E A L K K E L D P Y R L S C V A F Query_1 1399 GATTACCATAAGCAAGCTATTGTGTTGGCTAGGGAATTGGAGGCTTTGAAAAAAGAGCTGGATCCTTATCGTCTTTCTTGTGTCGCCTTT 1488 0 1198 .......................................................................................... 1287 H A F T W E K P Y T Q S S K E M F N I S D V N G V N V Y E S Query_1 1489 CATGCTTTTACTTGGGAGAAACCCTATACACAGTCTAGTAAGGAGATGTTTAATATTTCGGATGTTAACGGGGTAAATGTATATGAATCT 1578 0 1288 .......................................................................................... 1377 W Y Q G D S A T I A P M F D K F C S Y S T A K P R F L S Q F Query_1 1579 TGGTATCAGGGAGATTCTGCTACGATTGCACCTATGTTTGATAAATTTTGTAGTTATTCTACTGCAAAACCTCGTTTTTTGTCGCAGTTT 1668 0 1378 ....................................................................................G.A... 1467 G A G S D E R I H S Y T P R T F D F T P E F Q L D F N R R Y Query_1 1669 GGAGCAGGAAGCGATGAACGTATCCATTCTTATACTCCGCGTACATTTGATTTTACTCCGGAATTTCAATTGGATTTTAATCGCAGATAT 1758 0 1468 .......................................................................................... 1557 I N E M E K R P D Y I G Y S I W N L V D F Q V D G R G Query_1 1759 ATCAATGAAATGGAG--AAACGTCCGGA-TTATATAGGTTATTCTATTTGGAATTTGGTTGATTTTCA--GGTGGACGGTC--GTGGTGA 1841 0 1558 ...............--...........-.......................................--...........--....... 1640 1 1172 ..C.N....N....NG....N...C.NG....N..N.G.NN.T...N.....A.N..NNAN....NCNG..G........NG....-.N 1085 D S K P N L N Q K G M L T E D R R K K E I Y Y Y C Q A Query_1 1842 TAGC--AAGCCTAATCTGAATCAAAAA-GGAATGTTGAC---AGAGGATCGTCGGAAG-AAAGAGATTTATTA--TTACTGTCAGGC-TC 1921 0 1641 ....--.....................-...........---................-..............--............-.. 1720 1 1084 N..NCN.G.....N.NGN.T.......N.....N..T.NCCA..GN.N.NT..N...NN...NNN.......TNT.....T..N.N.N.. 995 R W S D I P M I H I A G A D W T K R V E I C D D S Query_1 1922 GTTGGTCGGA-CATTCCG--ATGATT-CAT--ATTGCCGGGG-CCGATTGGACGAAAA--GGGTGG--AAAT-TTGTG-ATGATAG-TA- 1996 0 1721 ..........-.......--......-...--..........-...............--......--....-.....-.......-..- 1795 1 994 .......N..N.N.....GA..N...T..NTNN..C......G...............AA......GA....N.....N.......G..N 905 I N V R K I S V F S N Q K T V E L I H N G K S L G V Query_1 1997 TAAATGTG--CGTAA-GATC-AGTGTTTTTT-CTAAT-CAGAAAA--CGGTGG-AGTTAATT-CATAATGG-AAAGTCTTT-GGGAGTGC 2074 0 1796 ........--.....-....-..........-.....-.......--......-........-........-.........-........ 1873 1 904 .....NANGN.....A.N..C..........T.....T.......AN......GN.......T.N......G.........N........ 815 R E V V N G E A V F V V P F I N G E N L L D A R S G A L Query_1 2075 -GTGAA-GTGGTAAA-TGGGGAA-GCTGTTTTCGTAGTTCCTTTTATAAAC-GGAGAGAATCTGCTGGATGCCCGAAGTGGGGCACTTTC 2159 0 1874 -.....-........-.......-...........................-...................................... 1958 1 814 C.....A..N.....A.......A................T..........C...................................... 725 S D R L K I Q M R L L S S R L T D S D V L L D G L C I N L G Query_1 2160 AGACAGGCTGAAGATACAGATGAGATTGTTATCTTCCCGTCTTACTGACAGTGATGTGCTTTTGGACGGTCTTTGTATTAATTTGGGACA 2249 0 1959 .......................................................................................... 2048 1 724 ........N................................................................................. 635 Q E H C Y F I D P Q L Q E I W I P D K P Y T K G S W G Y M D Query_1 2250 GGAGCATTGTTA-TTTTATAGACCCGCAGCTTCAGGAAATATGGATACCCGATAAACCTTATACAAAGGGAAGTTGGGGATATATGG-AT 2337 0 2049 ............-..........................................................................-.. 2136 1 634 ............N..........................................................................G.. 545 G K P F N S W P G S S H D G V R Y G V G A D I K N T F L E P Query_1 2338 GGAAAACCTTTTAACTCCTGGCCGGGATCATCACATGATGGAGTTAGGTATGGAGTAGGGGCTGATATAAAAAATACTTTTTTAGAGCCT 2427 0 2137 .......................................................................................... 2226 1 544 .......................................................................................... 455 L F Q T F L I G T T C Y R L D V P D G V Y E I G F Y F T E P Query_1 2428 CTTTTTCAGACCTTTTTAATTGGTACGACTTGTTACCGGCTGGATGTTCCTGACGGAGTATATGAAATTGGCTTTTATTTCACGGAACCT 2517 0 2227 .......................................................................................... 2316 1 454 .......................................................................................... 365 F S K D E R K N I V R T G V S A E G Q R V F D V S V N G E K Query_1 2518 TTCAGTAAGGATGAACGGAAAAATATTGTGCGTACAGGTGTTTCTGCCGAGGGACAACGTGTATTTGATGTGTCAGTCAATGGAGAAAAA 2607 0 2317 .......................................................................................... 2406 1 364 .......................................................................................... 275 L I E S L N L A D S Y G E Q T A V V K T L V V N V R N H E G Query_1 2608 TTGATAGAGTCATTGAATCTGGCTGATTCTTATGGCGAGCAGACAGCTGTTGTAAAGACACTTGTGGTCAATGTGCGGAATCATGAAGGA 2697 0 2407 .......................................................................................... 2496 1 274 .......................................................................................... 185 L E I L L S P Q K G Q G V I S G L K V K K I R * H W K W I T Query_1 2698 CTGGAGATTCTGCTTTCTCCTCAAAAAGGACAAGGTGTTATTAGCGGATTAAAAGTAAAGAAAATACGATAACATTGGAAGTGGATAACG 2787 0 2497 ..................................................................... 2565 1 184 .......................................................................................... 95 D P N S S S V D K L A A A L E H H H H H H * D P A A N K A R Query_1 2788 GATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGA 2877 1 94 ..........................N..................---.......................................... 8 Query_1 2878 A 2878 1 7 . 7 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 14475606 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,953 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200125.105 (CMR200125.22-801.P(801).E428A.pMCSG68)
QVSFGEAKKFNENWLFSLSDDSLSTSVSYDDSKWRKLDLPHDWSVEGQLSPSLASCTGYLPGGIGWYRKHFQVTDKAARHYIYFEGAYNRSEVYLNGHLLGKRPNGYISFMYDMTPYLKEGNNVLAVRIDHSRYADSRWYTGSGIYRDVWMISAPDIHFAQWGIGWHATSLTDKQAVVAVDMEVEKHVKAAGALEVSAALYDAEGALVAQGRKKVADKAEGIAKETISLKVKKPRRWNLDAPYLYTLKTELLCDGKRIDGSSTNVGLRTLKFDANKGFALNGKWMKVKGVCLHHDAGVLGAVVPPEVWERRLRNLKEIGVNAIRMSHNPQAPVLYDLCDRMGFLVMDEASDEWEFPKRKWIKGWNVGEPSYDGTFDFFEEWIERDVTDMVRRDRNHPCIFLWSIGNEVDYPNDPYSHPILDGTTINQPMFGGYKPDAPDAMRIGKIAKRLAACVRAVDTSRPVTGALAGVVMSNQTEYPEAVDVVGYNYTENRYDEDHATYPDRVIYGSETGVGIEAWHAVRDKEFIFGQFLWTGTDYLGESGRWPSRGLYTGLLDFGSFPKPRGYFRASLWSEKPVTYVGTYPKHDWVSMDAWDLWNYEPGQLIRVVCYTNAPQARLLLNGKEVGQMKPYDDKSGIIYWDIPYQDGELRAEGCDATGKVLSSYAIKTSGRPYALRATVDKEVLSPDKATAHVTIEVVDEQGTIVKLGDNDVTCQIEGPARLLGLEGSNNGDMSDYTDNHHRAFHGRLLAYIQTTGEKGEIRVKLTSPLLKGTEVVLKAE
CAAGTATCTTTTGGCGAAGCCAAAAAATTTAATGAGAATTGGTTGTTCAGTTTGTCGGACGATAGTCTGAGCACTTCCGTATCGTATGACGATAGTAAGTGGCGAAAACTTGATTTGCCGCACGACTGGTCGGTAGAAGGACAGCTTTCTCCTTCCCTGGCAAGCTGTACCGGTTATTTGCCGGGGGGGATAGGCTGGTATCGGAAACATTTTCAGGTTACCGATAAGGCTGCGCGCCACTACATCTATTTTGAGGGAGCATATAACCGCAGCGAGGTTTATTTGAACGGTCACTTGCTCGGTAAACGTCCGAACGGATACATATCTTTTATGTATGACATGACTCCCTACCTGAAGGAAGGAAATAACGTGCTGGCAGTCCGTATTGACCATAGCCGTTATGCCGATTCACGCTGGTACACCGGTTCCGGAATTTACCGGGATGTGTGGATGATTTCCGCCCCGGATATTCATTTTGCTCAATGGGGAATAGGCTGGCACGCTACTTCGCTGACCGATAAGCAGGCGGTAGTGGCAGTTGATATGGAAGTCGAAAAACATGTGAAGGCTGCTGGTGCATTGGAGGTTTCTGCGGCACTTTATGATGCAGAAGGTGCGTTGGTAGCACAAGGCCGTAAGAAAGTGGCAGATAAAGCTGAGGGTATTGCGAAAGAAACAATATCACTGAAGGTGAAGAAGCCTCGCCGCTGGAATCTGGATGCACCTTATCTGTACACGCTGAAGACAGAGTTGCTTTGTGACGGGAAACGGATAGATGGCAGTTCGACGAATGTAGGTCTCCGTACATTGAAGTTTGATGCCAACAAGGGATTTGCCTTGAACGGAAAGTGGATGAAGGTGAAAGGAGTATGTCTGCATCATGATGCAGGTGTGCTGGGTGCGGTAGTTCCTCCTGAAGTATGGGAGCGTCGTCTGAGAAACCTGAAAGAGATTGGTGTGAATGCCATCCGTATGAGCCATAATCCGCAGGCACCGGTATTGTATGATTTATGTGACCGAATGGGCTTCCTGGTGATGGATGAAGCTTCGGATGAATGGGAATTCCCGAAACGGAAATGGATAAAGGGATGGAATGTCGGTGAACCAAGCTATGACGGAACATTTGATTTCTTTGAGGAATGGATTGAACGCGATGTGACGGATATGGTGCGTCGTGACCGGAATCACCCTTGTATATTCTTGTGGAGCATCGGTAATGAGGTAGACTATCCGAACGATCCTTATTCTCACCCTATTTTGGATGGCACAACCATTAATCAGCCTATGTTCGGTGGATACAAGCCTGATGCCCCGGATGCCATGCGTATCGGTAAGATTGCGAAGCGTCTGGCTGCCTGTGTTCGTGCGGTGGACACTTCACGTCCTGTTACCGGTGCATTGGCCGGAGTGGTGATGTCGAATCAGACAGAGTATCCTGAAGCAGTAGATGTAGTAGGATATAATTATACGGAAAACCGTTATGACGAAGATCATGCTACTTATCCTGACCGTGTGATTTACGGTAGTGAAACCGGTGTGGGAATTGAGGCTTGGCATGCAGTACGCGACAAGGAGTTTATTTTCGGACAGTTCCTCTGGACGGGTACAGACTATTTGGGTGAATCAGGCAGATGGCCTTCCAGAGGTTTGTATACTGGTTTGCTGGACTTTGGCAGCTTCCCTAAACCCCGTGGATACTTCCGTGCTTCGCTTTGGAGTGAAAAACCTGTGACTTATGTTGGTACGTATCCTAAGCACGACTGGGTATCAATGGATGCATGGGATTTGTGGAACTACGAACCGGGTCAGTTGATTCGGGTGGTTTGTTATACCAATGCACCGCAGGCTCGTTTGTTGCTGAACGGAAAAGAAGTGGGTCAGATGAAGCCTTACGATGATAAGAGTGGTATCATTTATTGGGATATTCCTTATCAGGATGGAGAATTGCGTGCAGAAGGTTGTGATGCAACTGGAAAGGTGCTTTCATCTTATGCAATCAAGACCTCAGGACGTCCATACGCCTTGCGGGCTACTGTGGATAAGGAAGTCTTGTCGCCGGATAAGGCTACTGCACATGTGACAATCGAAGTGGTGGATGAACAAGGTACTATCGTAAAATTAGGCGATAACGACGTAACTTGTCAGATTGAAGGTCCGGCACGCTTGTTGGGACTGGAAGGTTCAAATAACGGAGATATGAGTGATTATACAGATAATCACCACCGTGCTTTTCACGGACGTTTGCTGGCTTATATCCAGACTACAGGTGAGAAAGGTGAGATTCGTGTGAAACTGACTTCTCCTCTTTTGAAAGGAACGGAAGTGGTATTGAAGGCTGAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,886 total letters Query= CMR200125.105 Length=2745 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4212 0.0 1_04G_G7_CMR200125.105_T7term.seq 1858 0.0 2_07G_G7_CMR200125.105_T7S.seq 1615 0.0 * Q F P S R N N F V * L * E G D I H M H H H H H H S S G V D Query_1 52 TAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGAT 141 2 15 ..N..T.....N.............................................................................. 104 2 1308 ........... 1298 L W S H P Q F E K G T E N L Y F Q S N A Q V S F G E A K K F Query_1 142 CTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAAGTATCTTTTGGCGAAGCCAAAAAATTT 231 0 1 .............................. 30 2 105 .......................................................................................... 194 N E N W L F S L S D D S L S T S V S Y D D S K W R K L D L P Query_1 232 AATGAGAATTGGTTGTTCAGTTTGTCGGACGATAGTCTGAGCACTTCCGTATCGTATGACGATAGTAAGTGGCGAAAACTTGATTTGCCG 321 0 31 .......................................................................................... 120 2 195 .......................................................................................... 284 H D W S V E G Q L S P S L A S C T G Y L P G G I G W Y R K H Query_1 322 CACGACTGGTCGGTAGAAGGACAGCTTTCTCCTTCCCTGGCAAGCTGTACCGGTTATTTGCCgggggggATAGGCTGGTATCGGAAACAT 411 0 121 .......................................................................................... 210 2 285 .......................................................................................... 374 F Q V T D K A A R H Y I Y F E G A Y N R S E V Y L N G H L L Query_1 412 TTTCAGGTTACCGATAAGGCTGCGCGCCACTACATCTATTTTGAGGGAGCATATAACCGCAGCGAGGTTTATTTGAACGGTCACTTGCTC 501 0 211 .......................................................................................... 300 2 375 .......................................................................................... 464 G K R P N G Y I S F M Y D M T P Y L K E G N N V L A V R I D Query_1 502 GGTAAACGTCCGAACGGATACATATCTTTTATGTATGACATGACTCCCTACCTGAAGGAAGGAAATAACGTGCTGGCAGTCCGTATTGAC 591 0 301 .......................................................................................... 390 2 465 .......................................................................................... 554 H S R Y A D S R W Y T G S G I Y R D V W M I S A P D I H F A Query_1 592 CATAGCCGTTATGCCGATTCACGCTGGTACACCGGTTCCGGAATTTACCGGGATGTGTGGATGATTTCCGCCCCGGATATTCATTTTGCT 681 0 391 .......................................................................................... 480 2 555 .......................................................................................... 644 Q W G I G W H A T S L T D K Q A V V A V D M E V E K H V K Query_1 682 CAATGGGG-AATAGGCTGGCACGCTAC-TTCGCTGACCGATAAGCAGGCGGTAGTGGCAGTTGATATGGAAGTCG-AAAAACATGTGAAG 768 0 481 ........-..................-...............................................-.............. 567 2 645 ........A..................T...N...........................................A.............. 734 A A G A L E V S A A L Y D A E G A L V A Q G R K K V A D Query_1 769 GCTGCTGGTGCA-TTGGAGGTTTCTGCGGCAC-TTTATGATGCAGAAGGTGCGTT-GGTAGCACAAGGCCGTAAGAAAGTGGCAGAT-AA 854 0 568 ............-...................-......................-...............................-.. 653 2 735 ............T...................T...........N..........G....N.........N................A.. 824 K A E G I A K E T I S L K V K K P R R W N L D A P Y L Y T Query_1 855 AGCTGAGGGTATTGCGAAAGAAACAAT-ATCACTGAA-GGTGAAGAAGCCTCGCCGCTGGAATCT-GGATGCACCTTATCTGTACACGCT 941 0 654 ...........................-.........-...........................-........................ 740 2 825 ........................N..N.........N..NN.......................G.................N.N.... 914 L K T E L L C D G K R I D G S S T N V G L R T L K F D A Query_1 942 GAAGACAG-AGTTGCTTTGTGACGGGAAACGGAT-AGA-TGGCAG-TTCGACGAATG-TAGGTCT-CCGTACATTG-AAGTTTGATGCCA 1024 0 741 ........-.........................-...-......-...........-.......-..........-............. 823 2 915 ........A....................NN.G.G...T......NN.....T....T.......C....CA....A...........A. 1004 N K G F A L N G K W M K V K G V C L H H D A G V L G A Query_1 1025 AC-AAGGGATTTG-CCTTGAA-CGGAAAGTGGATGAAGGT-GAAAGGA--GTATGT-CTGCATCATGATGC-AGGTGTGCTGGGTGCGGT 1106 0 824 ..-..........-.......-..................-.......--......-..............-.................. 905 0 889 ..............-.. 874 2 1005 ..A......N...C.......N...N..NG...A......CN......AGA....NC......N.......N..NN....C... 1088 V V P P E V W E R R L R N L K E I G V N A I R M S H N P Q A Query_1 1107 AGTTCCTCCTGAAGTATGGGAGCGTCGTCTGAGAAACCTGAAAGAGATTGGTGTGAATGCCATCCGTATGAGCCATAATCCGCAGGCACC 1196 0 906 .......................................................................................... 995 P V L Y D L C D R M G F L V M D E A S D E W E F P K R K W I Query_1 1197 GGTATTGTATGATTTATGTGACCGAATGGGCTTCCTGGTGATGGATGAAGCTTCGGATGAATGGGAATTCCCGAAACGGAAATGGATAAA 1286 0 996 .......................................................................................... 1085 K G W N V G E P S Y D G T F D F F E E W I E R D V T D M V R Query_1 1287 GGGATGGAATGTCGGTGAACCAAGCTATGACGGAACATTTGATTTCTTTGAGGAATGGATTGAACGCGATGTGACGGATATGGTGCGTCG 1376 0 1086 .......................................................................................... 1175 R D R N H P C I F L W S I G N A V D Y P N D P Y S H P I L D Query_1 1377 TGACCGGAATCACCCTTGTATATTCTTGTGGAGCATCGGTAATGCTGTAGACTATCCGAACGATCCTTATTCTCACCCTATTTTGGATGG 1466 0 1176 ............................................AG............................................ 1265 G T T I N Q P M F G G Y K P D A P D A M R I G K I A K R L Query_1 1467 CACAACCATTAATCAGCCTATGTTCGGTGGATACAAGCCTGAT-GCCCCGGATGCCA-TGCGTATCGGTAAGATTGCGAA-GCGT-CTGG 1552 0 1266 ...........................................-.............-......................-....-.... 1351 1 1175 ...N...T....G....TNN.A.NN.N....T...NN...N...A....T.N.. 1122 A A C V R A V D T S R P V T G A L A G V V M S N Q T E Y P Query_1 1553 CTGCCTGTGT-TCGTGCGGTGGA-CACTTCACGTCCTGTTACC-GGTGCATTGGCCGGAGTGGT-GATGTCGAATCAGACAGAGTATCCT 1638 0 1352 ..........-............-...................-....................-......................... 1437 1 1121 ...NN.....A..NNN....N..A.NN.NA......N......C.......G.--...N.N..NN...N.........N........... 1034 1 633 .-.......... 643 E A V D V V G Y N Y T E N R Y D E D H A T Y P D R V I Y G S Query_1 1639 GAAGCAGTAGATGTAGTAGGATATAATTATACGGAAAACCGTTATGACGAAGATCATGCTACTTATCCTGACCGTGTGATTTACGGTAGT 1728 0 1438 .......................................................................................... 1527 1 1033 ............NN...........-................-...............................N............... 946 E T G V G I E A W H A V R D K E F I F G Q F L W T G T D Y L Query_1 1729 GAAACCGGTGTGGGAATTGAGGCTTGGCATGCAGTACGCGACAAGGAGTTTATTTTCGGACAGTTCCTCTGGACGGGTACAGACTATTTG 1818 0 1528 .......................................................................................... 1617 1 945 .......................................................................................... 856 G E S G R W P S R G L Y T G L L D F G S F P K P R G Y F R A Query_1 1819 GGTGAATCAGGCAGATGGCCTTCCAGAGGTTTGTATACTGGTTTGCTGGACTTTGGCAGCTTCCCTAAACCCCGTGGATACTTCCGTGCT 1908 0 1618 .......................................................................................... 1707 1 855 .....................................................-..............-..................... 768 S L W S E K P V T Y V G T Y P K H D W V S M D A W D L W N Y Query_1 1909 TCGCTTTGGAGTGAAAAACCTGTGACTTATGTTGGTACGTATCCTAAGCACGACTGGGTATCAATGGATGCATGGGATTTGTGGAACTAC 1998 0 1708 .......................................................................................... 1797 1 767 .......................................................................................... 678 E P G Q L I R V V C Y T N A P Q A R L L L N G K E V G Q M K Query_1 1999 GAACCGGGTCAGTTGATTCGGGTGGTTTGTTATACCAATGCACCGCAGGCTCGTTTGTTGCTGAACGGAAAAGAAGTGGGTCAGATGAAG 2088 0 1798 .......................................................................................... 1887 1 677 ..............................................G........................................... 588 P Y D D K S G I I Y W D I P Y Q D G E L R A E G C D A T G K Query_1 2089 CCTTACGATGATAAGAGTGGTATCATTTATTGGGATATTCCTTATCAGGATGGAGAATTGCGTGCAGAAGGTTGTGATGCAACTGGAAAG 2178 0 1888 .......................................................................................... 1977 1 587 .......................................................................................... 498 V L S S Y A I K T S G R P Y A L R A T V D K E V L S P D K A Query_1 2179 GTGCTTTCATCTTATGCAATCAAGACCTCAGGACGTCCATACGCCTTGCGGGCTACTGTGGATAAGGAAGTCTTGTCGCCGGATAAGGCT 2268 0 1978 .......................................................................................... 2067 1 497 .......................................................................................... 408 T A H V T I E V V D E Q G T I V K L G D N D V T C Q I E G P Query_1 2269 ACTGCACATGTGACAATCGAAGTGGTGGATGAACAAGGTACTATCGTAAAATTAGGCGATAACGACGTAACTTGTCAGATTGAAGGTCCG 2358 0 2068 .......................................................................................... 2157 1 407 .......................................................................................... 318 A R L L G L E G S N N G D M S D Y T D N H H R A F H G R L L Query_1 2359 GCACGCTTGTTGGGACTGGAAGGTTCAAATAACGGAGATATGAGTGATTATACAGATAATCACCACCGTGCTTTTCACGGACGTTTGCTG 2448 0 2158 .......................................................................................... 2247 1 317 .......................................................................................... 228 A Y I Q T T G E K G E I R V K L T S P L L K G T E V V L K A Query_1 2449 GCTTATATCCAGACTACAGGTGAGAAAGGTGAGATTCGTGTGAAACTGACTTCTCCTCTTTTGAAAGGAACGGAAGTGGTATTGAAGGCT 2538 0 2248 .......................................................................................... 2337 1 227 .......................................................................................... 138 E * H W K W I T D P N S S S V D K L A A A L E H H H H H H * Query_1 2539 GAGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGA 2628 0 2338 ... 2340 1 137 .......................................................................................... 48 D P A A N K A R K Query_1 2629 GATCCGGCTGCTAACAAAGCCCGAAAGG 2656 1 47 ............................ 20 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 13189880 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,886 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200125.106 (CMR200125.22-801.P(801).E531A.pMCSG68)
QVSFGEAKKFNENWLFSLSDDSLSTSVSYDDSKWRKLDLPHDWSVEGQLSPSLASCTGYLPGGIGWYRKHFQVTDKAARHYIYFEGAYNRSEVYLNGHLLGKRPNGYISFMYDMTPYLKEGNNVLAVRIDHSRYADSRWYTGSGIYRDVWMISAPDIHFAQWGIGWHATSLTDKQAVVAVDMEVEKHVKAAGALEVSAALYDAEGALVAQGRKKVADKAEGIAKETISLKVKKPRRWNLDAPYLYTLKTELLCDGKRIDGSSTNVGLRTLKFDANKGFALNGKWMKVKGVCLHHDAGVLGAVVPPEVWERRLRNLKEIGVNAIRMSHNPQAPVLYDLCDRMGFLVMDEASDEWEFPKRKWIKGWNVGEPSYDGTFDFFEEWIERDVTDMVRRDRNHPCIFLWSIGNEVDYPNDPYSHPILDGTTINQPMFGGYKPDAPDAMRIGKIAKRLAACVRAVDTSRPVTGALAGVVMSNQTEYPEAVDVVGYNYTENRYDEDHATYPDRVIYGSETGVGIEAWHAVRDKEFIFGQFLWTGTDYLGESGRWPSRGLYTGLLDFGSFPKPRGYFRASLWSEKPVTYVGTYPKHDWVSMDAWDLWNYEPGQLIRVVCYTNAPQARLLLNGKEVGQMKPYDDKSGIIYWDIPYQDGELRAEGCDATGKVLSSYAIKTSGRPYALRATVDKEVLSPDKATAHVTIEVVDEQGTIVKLGDNDVTCQIEGPARLLGLEGSNNGDMSDYTDNHHRAFHGRLLAYIQTTGEKGEIRVKLTSPLLKGTEVVLKAE
CAAGTATCTTTTGGCGAAGCCAAAAAATTTAATGAGAATTGGTTGTTCAGTTTGTCGGACGATAGTCTGAGCACTTCCGTATCGTATGACGATAGTAAGTGGCGAAAACTTGATTTGCCGCACGACTGGTCGGTAGAAGGACAGCTTTCTCCTTCCCTGGCAAGCTGTACCGGTTATTTGCCGGGGGGGATAGGCTGGTATCGGAAACATTTTCAGGTTACCGATAAGGCTGCGCGCCACTACATCTATTTTGAGGGAGCATATAACCGCAGCGAGGTTTATTTGAACGGTCACTTGCTCGGTAAACGTCCGAACGGATACATATCTTTTATGTATGACATGACTCCCTACCTGAAGGAAGGAAATAACGTGCTGGCAGTCCGTATTGACCATAGCCGTTATGCCGATTCACGCTGGTACACCGGTTCCGGAATTTACCGGGATGTGTGGATGATTTCCGCCCCGGATATTCATTTTGCTCAATGGGGAATAGGCTGGCACGCTACTTCGCTGACCGATAAGCAGGCGGTAGTGGCAGTTGATATGGAAGTCGAAAAACATGTGAAGGCTGCTGGTGCATTGGAGGTTTCTGCGGCACTTTATGATGCAGAAGGTGCGTTGGTAGCACAAGGCCGTAAGAAAGTGGCAGATAAAGCTGAGGGTATTGCGAAAGAAACAATATCACTGAAGGTGAAGAAGCCTCGCCGCTGGAATCTGGATGCACCTTATCTGTACACGCTGAAGACAGAGTTGCTTTGTGACGGGAAACGGATAGATGGCAGTTCGACGAATGTAGGTCTCCGTACATTGAAGTTTGATGCCAACAAGGGATTTGCCTTGAACGGAAAGTGGATGAAGGTGAAAGGAGTATGTCTGCATCATGATGCAGGTGTGCTGGGTGCGGTAGTTCCTCCTGAAGTATGGGAGCGTCGTCTGAGAAACCTGAAAGAGATTGGTGTGAATGCCATCCGTATGAGCCATAATCCGCAGGCACCGGTATTGTATGATTTATGTGACCGAATGGGCTTCCTGGTGATGGATGAAGCTTCGGATGAATGGGAATTCCCGAAACGGAAATGGATAAAGGGATGGAATGTCGGTGAACCAAGCTATGACGGAACATTTGATTTCTTTGAGGAATGGATTGAACGCGATGTGACGGATATGGTGCGTCGTGACCGGAATCACCCTTGTATATTCTTGTGGAGCATCGGTAATGAGGTAGACTATCCGAACGATCCTTATTCTCACCCTATTTTGGATGGCACAACCATTAATCAGCCTATGTTCGGTGGATACAAGCCTGATGCCCCGGATGCCATGCGTATCGGTAAGATTGCGAAGCGTCTGGCTGCCTGTGTTCGTGCGGTGGACACTTCACGTCCTGTTACCGGTGCATTGGCCGGAGTGGTGATGTCGAATCAGACAGAGTATCCTGAAGCAGTAGATGTAGTAGGATATAATTATACGGAAAACCGTTATGACGAAGATCATGCTACTTATCCTGACCGTGTGATTTACGGTAGTGAAACCGGTGTGGGAATTGAGGCTTGGCATGCAGTACGCGACAAGGAGTTTATTTTCGGACAGTTCCTCTGGACGGGTACAGACTATTTGGGTGAATCAGGCAGATGGCCTTCCAGAGGTTTGTATACTGGTTTGCTGGACTTTGGCAGCTTCCCTAAACCCCGTGGATACTTCCGTGCTTCGCTTTGGAGTGAAAAACCTGTGACTTATGTTGGTACGTATCCTAAGCACGACTGGGTATCAATGGATGCATGGGATTTGTGGAACTACGAACCGGGTCAGTTGATTCGGGTGGTTTGTTATACCAATGCACCGCAGGCTCGTTTGTTGCTGAACGGAAAAGAAGTGGGTCAGATGAAGCCTTACGATGATAAGAGTGGTATCATTTATTGGGATATTCCTTATCAGGATGGAGAATTGCGTGCAGAAGGTTGTGATGCAACTGGAAAGGTGCTTTCATCTTATGCAATCAAGACCTCAGGACGTCCATACGCCTTGCGGGCTACTGTGGATAAGGAAGTCTTGTCGCCGGATAAGGCTACTGCACATGTGACAATCGAAGTGGTGGATGAACAAGGTACTATCGTAAAATTAGGCGATAACGACGTAACTTGTCAGATTGAAGGTCCGGCACGCTTGTTGGGACTGGAAGGTTCAAATAACGGAGATATGAGTGATTATACAGATAATCACCACCGTGCTTTTCACGGACGTTTGCTGGCTTATATCCAGACTACAGGTGAGAAAGGTGAGATTCGTGTGAAACTGACTTCTCCTCTTTTGAAAGGAACGGAAGTGGTATTGAAGGCTGAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,318 total letters Query= CMR200125.106 Length=2745 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4212 0.0 1_04H_H7_CMR200125.106_T7term.seq 1763 0.0 2_07H_H7_CMR200125.106_T7S.seq 1296 0.0 F V * L * E G D I H M H H H H H H S S G V D L W S H P Q F Query_1 75 TTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGA 164 2 19 .........-..................................N..N.......................................... 107 E K G T E N L Y F Q S N A Q V S F G E A K K F N E N W L F S Query_1 165 AAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAAGTATCTTTTGGCGAAGCCAAAAAATTTAATGAGAATTGGTTGTTCAGTTT 254 0 1 ..................................................... 53 2 108 .......................................................................................... 197 L S D D S L S T S V S Y D D S K W R K L D L P H D W S V E G Query_1 255 GTCGGACGATAGTCTGAGCACTTCCGTATCGTATGACGATAGTAAGTGGCGAAAACTTGATTTGCCGCACGACTGGTCGGTAGAAGGACA 344 0 54 .......................................................................................... 143 2 198 .......................................................................................... 287 Q L S P S L A S C T G Y L P G G I G W Y R K H F Q V T D K A Query_1 345 GCTTTCTCCTTCCCTGGCAAGCTGTACCGGTTATTTGCCgggggggATAGGCTGGTATCGGAAACATTTTCAGGTTACCGATAAGGCTGC 434 0 144 .......................................................................................... 233 2 288 .......................................................................................... 377 A R H Y I Y F E G A Y N R S E V Y L N G H L L G K R P N G Y Query_1 435 GCGCCACTACATCTATTTTGAGGGAGCATATAACCGCAGCGAGGTTTATTTGAACGGTCACTTGCTCGGTAAACGTCCGAACGGATACAT 524 0 234 .......................................................................................... 323 2 378 .......................................................................................... 467 I S F M Y D M T P Y L K E G N N V L A V R I D H S R Y A D S Query_1 525 ATCTTTTATGTATGACATGACTCCCTACCTGAAGGAAGGAAATAACGTGCTGGCAGTCCGTATTGACCATAGCCGTTATGCCGATTCACG 614 0 324 .......................................................................................... 413 2 468 .......................................................................................... 557 R W Y T G S G I Y R D V W M I S A P D I H F A Q W G I G W H Query_1 615 CTGGTACACCGGTTCCGGAATTTACCGGGATGTGTGGATGATTTCCGCCCCGGATATTCATTTTGCTCAATGGGGAATAGGCTGGCACGC 704 0 414 .......................................................................................... 503 2 558 .......................................................................................... 647 A T S L T D K Q A V V A V D M E V E K H V K A A G A L E V S Query_1 705 TAC-TTCGCTGACCGATAAGCAGGCGGTAGTGGCAGTTGATATGGAAGTCGAAAAACATGTGAAGGCTGCTGGTGCATTGGAGGTTTCTG 793 0 504 ...-...................................................................................... 592 2 648 ...T.............................-.............N....................................-..... 735 A A L Y D A E G A L V A Q G R K K V A D K A E G I A K E T I Query_1 794 CGGCACTTTATGATGCAGAAGGTGCGTTGGTAGCACAAGGCCGTAAGAAAGTGGCAGATAAAGCTGAGGGTATTGCGAAAGAAACAATAT 883 0 593 .......................................................................................... 682 2 736 .........N.............. 759 S L K V K K P R R W N L D A P Y L Y T L K T E L L C D G K R Query_1 884 CACTGAAGGTGAAGAAGCCTCGCCGCTGGAATCTGGATGCACCTTATCTGTACACGCTGAAGACAGAGTTGCTTTGTGACGGGAAACGGA 973 0 683 .......................................................................................... 772 I D G S S T N V G L R T L K F D A N K G F A L N G K W M K V Query_1 974 TAGATGGCAGTTCGACGAATGTAGGTCTCCGTACATTGAAGTTTGATGCCAACAAGGGATTTGCCTTGAACGGAAAGTGGATGAAGGTGA 1063 0 773 .......................................................................................... 862 K G V C L H H D A G V L G A V V P P E V W E R R L R N L K E Query_1 1064 AAGGAGTATGTCTGCATCATGATGCAGGTGTGCTGGGTGCGGTAGTTCCTCCTGAAGTATGGGAGCGTCGTCTGAGAAACCTGAAAGAGA 1153 0 863 .......................................................................................... 952 0 889 ................ 874 I G V N A I R M S H N P Q A P V L Y D L C D R M G F L V M D Query_1 1154 TTGGTGTGAATGCCATCCGTATGAGCCATAATCCGCAGGCACCGGTATTGTATGATTTATGTGACCGAATGGGCTTCCTGGTGATGGATG 1243 0 953 .......................................................................................... 1042 E A S D E W E F P K R K W I K G W N V G E P S Y D G T F D F Query_1 1244 AAGCTTCGGATGAATGGGAATTCCCGAAACGGAAATGGATAAAGGGATGGAATGTCGGTGAACCAAGCTATGACGGAACATTTGATTTCT 1333 0 1043 .......................................................................................... 1132 F E E W I E R D V T D M V R R D R N H P C I F L W S I G N E Query_1 1334 TTGAGGAATGGATTGAACGCGATGTGACGGATATGGTGCGTCGTGACCGGAATCACCCTTGTATATTCTTGTGGAGCATCGGTAATGAGG 1423 0 1133 .......................................................................................... 1222 V D Y P N D P Y S H P I L D G T T I N Q P M F G G Y K P D A Query_1 1424 TAGACTATCCGAACGATCCTTATTCTCACCCTATTTTGGATGGCACAACCATTAATCAGCCTATGTTCGGTGGATACAAGCCTGATGCCC 1513 0 1223 .......................................................................................... 1312 P D A M R I G K I A K R L A A C V R A V D T S R P V T G A Query_1 1514 CGGATGCCATGCGTATCGGTAAGATTGCGAAGCGTCTGGCTGCCTGTGTTCGTGCGGTGGACACTTCACGTCCTGTTACCGGT--GCATT 1601 0 1313 ...................................................................................--..... 1400 0 1842 ....--..... 1834 1 1117 ..N...NN..N....N.C.NNN..... 1091 1 635 ....--..... 643 L A G V V M S N Q T E Y P E A V D V V G Y N Y T E N R Y Query_1 1602 GGCCGGAGTGG-TGATGTCGAA--TCAGACAGAGTATCCTGAAGCAGT-AGATG-TAGTAGGATATAATT-ATACGGAAAA-CCGTTATG 1684 0 1401 ...........-..........--........................-.....-...............-..........-........ 1483 0 1833 .. 1832 1 1090 N.NG.......GN.......N.AT......N....N............TN....N......N........T..........A....A... 1001 1 644 .. 645 D E D H A T Y P D R V I Y G S A T G V G I E A W H A V R Query_1 1685 ACGA-AGAT-CATGCTACTTATCC-TGACCGTGTGATTTAC--GGTAGTGCTACC-GGTGTGGGAATTGAGGCTTGGCATGCAGTACGCG 1768 0 1484 ....-....-..............-................--.......AA...-.................................. 1567 1 1000 ....T....T..............N...............NCG............C.................................. 911 D K E F I F G Q F L W T G T D Y L G E S G R W P S R G L Y Query_1 1769 ACAA-GGAGTTTATTTTCGGACAGTTCCTCTGGACGGGTACAGACTATTTGGGTGAATCAGGCAGATGGCCTT-CCAGAGGTTTGTATAC 1856 0 1568 ....-....................................................................-................ 1655 1 910 ....A...............................................-....................T................ 822 T G L L D F G S F P K P R G Y F R A S L W S E K P V T Y V G Query_1 1857 TGGTTTGCTGGACTTTGGCAGCTTCCCTAAACCCCGTGGATACTTCCGTGCTTCGCTTTGGAGTGAAAAACCTGTGACTTATGTTGGTAC 1946 0 1656 .......................................................................................... 1745 1 821 .......................................................................................... 732 T Y P K H D W V S M D A W D L W N Y E P G Q L I R V V C Y T Query_1 1947 GTATCCTAAGCACGACTGGGTATCAATGGATGCATGGGATTTGTGGAACTACGAACCGGGTCAGTTGATTCGGGTGGTTTGTTATACCAA 2036 0 1746 .......................................................................................... 1835 0 1402 .... 1399 1 731 .......................................................T.................................. 642 N A P Q A R L L L N G K E V G Q M K P Y D D K S G I I Y W D Query_1 2037 TGCACCGCAGGCTCGTTTGTTGCTGAACGGAAAAGAAGTGGGTCAGATGAAGCCTTACGATGATAAGAGTGGTATCATTTATTGGGATAT 2126 0 1836 .......................................................................................... 1925 0 1398 ....... 1392 1 641 .......................................................................................... 552 I P Y Q D G E L R A E G C D A T G K V L S S Y A I K T S G R Query_1 2127 TCCTTATCAGGATGGAGAATTGCGTGCAGAAGGTTGTGATGCAACTGGAAAGGTGCTTTCATCTTATGCAATCAAGACCTCAGGACGTCC 2216 0 1926 .......................................................................................... 2015 1 551 .......................................................................................... 462 P Y A L R A T V D K E V L S P D K A T A H V T I E V V D E Q Query_1 2217 ATACGCCTTGCGGGCTACTGTGGATAAGGAAGTCTTGTCGCCGGATAAGGCTACTGCACATGTGACAATCGAAGTGGTGGATGAACAAGG 2306 0 2016 .......................................................................................... 2105 1 461 .......................................................................................... 372 G T I V K L G D N D V T C Q I E G P A R L L G L E G S N N G Query_1 2307 TACTATCGTAAAATTAGGCGATAACGACGTAACTTGTCAGATTGAAGGTCCGGCACGCTTGTTGGGACTGGAAGGTTCAAATAACGGAGA 2396 0 2106 .......................................................................................... 2195 1 371 .......................................................................................... 282 D M S D Y T D N H H R A F H G R L L A Y I Q T T G E K G E I Query_1 2397 TATGAGTGATTATACAGATAATCACCACCGTGCTTTTCACGGACGTTTGCTGGCTTATATCCAGACTACAGGTGAGAAAGGTGAGATTCG 2486 0 2196 .......................................................................................... 2285 1 281 .......................................................................................... 192 R V K L T S P L L K G T E V V L K A E * H W K W I T D P N S Query_1 2487 TGTGAAACTGACTTCTCCTCTTTTGAAAGGAACGGAAGTGGTATTGAAGGCTGAGTAACATTGGAAGTGGATAACGGATCCGAATTCGAG 2576 0 2286 ....................................................... 2340 1 191 .......................................................................................... 102 S S V D K L A A A L E H H H H H H * D P A A N K A Query_1 2577 CTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCC 2650 1 101 .......................................................................... 28 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11640376 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,318 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200125.107 (CMR200125.22-801.P(801).E428Q.pMCSG68)
QVSFGEAKKFNENWLFSLSDDSLSTSVSYDDSKWRKLDLPHDWSVEGQLSPSLASCTGYLPGGIGWYRKHFQVTDKAARHYIYFEGAYNRSEVYLNGHLLGKRPNGYISFMYDMTPYLKEGNNVLAVRIDHSRYADSRWYTGSGIYRDVWMISAPDIHFAQWGIGWHATSLTDKQAVVAVDMEVEKHVKAAGALEVSAALYDAEGALVAQGRKKVADKAEGIAKETISLKVKKPRRWNLDAPYLYTLKTELLCDGKRIDGSSTNVGLRTLKFDANKGFALNGKWMKVKGVCLHHDAGVLGAVVPPEVWERRLRNLKEIGVNAIRMSHNPQAPVLYDLCDRMGFLVMDEASDEWEFPKRKWIKGWNVGEPSYDGTFDFFEEWIERDVTDMVRRDRNHPCIFLWSIGNEVDYPNDPYSHPILDGTTINQPMFGGYKPDAPDAMRIGKIAKRLAACVRAVDTSRPVTGALAGVVMSNQTEYPEAVDVVGYNYTENRYDEDHATYPDRVIYGSETGVGIEAWHAVRDKEFIFGQFLWTGTDYLGESGRWPSRGLYTGLLDFGSFPKPRGYFRASLWSEKPVTYVGTYPKHDWVSMDAWDLWNYEPGQLIRVVCYTNAPQARLLLNGKEVGQMKPYDDKSGIIYWDIPYQDGELRAEGCDATGKVLSSYAIKTSGRPYALRATVDKEVLSPDKATAHVTIEVVDEQGTIVKLGDNDVTCQIEGPARLLGLEGSNNGDMSDYTDNHHRAFHGRLLAYIQTTGEKGEIRVKLTSPLLKGTEVVLKAE
CAAGTATCTTTTGGCGAAGCCAAAAAATTTAATGAGAATTGGTTGTTCAGTTTGTCGGACGATAGTCTGAGCACTTCCGTATCGTATGACGATAGTAAGTGGCGAAAACTTGATTTGCCGCACGACTGGTCGGTAGAAGGACAGCTTTCTCCTTCCCTGGCAAGCTGTACCGGTTATTTGCCGGGGGGGATAGGCTGGTATCGGAAACATTTTCAGGTTACCGATAAGGCTGCGCGCCACTACATCTATTTTGAGGGAGCATATAACCGCAGCGAGGTTTATTTGAACGGTCACTTGCTCGGTAAACGTCCGAACGGATACATATCTTTTATGTATGACATGACTCCCTACCTGAAGGAAGGAAATAACGTGCTGGCAGTCCGTATTGACCATAGCCGTTATGCCGATTCACGCTGGTACACCGGTTCCGGAATTTACCGGGATGTGTGGATGATTTCCGCCCCGGATATTCATTTTGCTCAATGGGGAATAGGCTGGCACGCTACTTCGCTGACCGATAAGCAGGCGGTAGTGGCAGTTGATATGGAAGTCGAAAAACATGTGAAGGCTGCTGGTGCATTGGAGGTTTCTGCGGCACTTTATGATGCAGAAGGTGCGTTGGTAGCACAAGGCCGTAAGAAAGTGGCAGATAAAGCTGAGGGTATTGCGAAAGAAACAATATCACTGAAGGTGAAGAAGCCTCGCCGCTGGAATCTGGATGCACCTTATCTGTACACGCTGAAGACAGAGTTGCTTTGTGACGGGAAACGGATAGATGGCAGTTCGACGAATGTAGGTCTCCGTACATTGAAGTTTGATGCCAACAAGGGATTTGCCTTGAACGGAAAGTGGATGAAGGTGAAAGGAGTATGTCTGCATCATGATGCAGGTGTGCTGGGTGCGGTAGTTCCTCCTGAAGTATGGGAGCGTCGTCTGAGAAACCTGAAAGAGATTGGTGTGAATGCCATCCGTATGAGCCATAATCCGCAGGCACCGGTATTGTATGATTTATGTGACCGAATGGGCTTCCTGGTGATGGATGAAGCTTCGGATGAATGGGAATTCCCGAAACGGAAATGGATAAAGGGATGGAATGTCGGTGAACCAAGCTATGACGGAACATTTGATTTCTTTGAGGAATGGATTGAACGCGATGTGACGGATATGGTGCGTCGTGACCGGAATCACCCTTGTATATTCTTGTGGAGCATCGGTAATGAGGTAGACTATCCGAACGATCCTTATTCTCACCCTATTTTGGATGGCACAACCATTAATCAGCCTATGTTCGGTGGATACAAGCCTGATGCCCCGGATGCCATGCGTATCGGTAAGATTGCGAAGCGTCTGGCTGCCTGTGTTCGTGCGGTGGACACTTCACGTCCTGTTACCGGTGCATTGGCCGGAGTGGTGATGTCGAATCAGACAGAGTATCCTGAAGCAGTAGATGTAGTAGGATATAATTATACGGAAAACCGTTATGACGAAGATCATGCTACTTATCCTGACCGTGTGATTTACGGTAGTGAAACCGGTGTGGGAATTGAGGCTTGGCATGCAGTACGCGACAAGGAGTTTATTTTCGGACAGTTCCTCTGGACGGGTACAGACTATTTGGGTGAATCAGGCAGATGGCCTTCCAGAGGTTTGTATACTGGTTTGCTGGACTTTGGCAGCTTCCCTAAACCCCGTGGATACTTCCGTGCTTCGCTTTGGAGTGAAAAACCTGTGACTTATGTTGGTACGTATCCTAAGCACGACTGGGTATCAATGGATGCATGGGATTTGTGGAACTACGAACCGGGTCAGTTGATTCGGGTGGTTTGTTATACCAATGCACCGCAGGCTCGTTTGTTGCTGAACGGAAAAGAAGTGGGTCAGATGAAGCCTTACGATGATAAGAGTGGTATCATTTATTGGGATATTCCTTATCAGGATGGAGAATTGCGTGCAGAAGGTTGTGATGCAACTGGAAAGGTGCTTTCATCTTATGCAATCAAGACCTCAGGACGTCCATACGCCTTGCGGGCTACTGTGGATAAGGAAGTCTTGTCGCCGGATAAGGCTACTGCACATGTGACAATCGAAGTGGTGGATGAACAAGGTACTATCGTAAAATTAGGCGATAACGACGTAACTTGTCAGATTGAAGGTCCGGCACGCTTGTTGGGACTGGAAGGTTCAAATAACGGAGATATGAGTGATTATACAGATAATCACCACCGTGCTTTTCACGGACGTTTGCTGGCTTATATCCAGACTACAGGTGAGAAAGGTGAGATTCGTGTGAAACTGACTTCTCCTCTTTTGAAAGGAACGGAAGTGGTATTGAAGGCTGAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,820 total letters Query= CMR200125.107 Length=2745 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4215 0.0 2_08A_A8_CMR200125.107_T7S.seq 1945 0.0 1_05A_A8_CMR200125.107_T7term.seq 1894 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W S Query_1 61 CCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCT 150 2 12 ..........-............................................................................... 100 H P Q F E K G T E N L Y F Q S N A Q V S F G E A K K F N E N Query_1 151 CATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAAGTATCTTTTGGCGAAGCCAAAAAATTTAATGAGAAT 240 0 1 ....................................... 39 2 101 .......................................................................................... 190 W L F S L S D D S L S T S V S Y D D S K W R K L D L P H D W Query_1 241 TGGTTGTTCAGTTTGTCGGACGATAGTCTGAGCACTTCCGTATCGTATGACGATAGTAAGTGGCGAAAACTTGATTTGCCGCACGACTGG 330 0 40 .......................................................................................... 129 2 191 .......................................................................................... 280 S V E G Q L S P S L A S C T G Y L P G G I G W Y R K H F Q V Query_1 331 TCGGTAGAAGGACAGCTTTCTCCTTCCCTGGCAAGCTGTACCGGTTATTTGCCgggggggATAGGCTGGTATCGGAAACATTTTCAGGTT 420 0 130 .......................................................................................... 219 2 281 .......................................................................................... 370 T D K A A R H Y I Y F E G A Y N R S E V Y L N G H L L G K R Query_1 421 ACCGATAAGGCTGCGCGCCACTACATCTATTTTGAGGGAGCATATAACCGCAGCGAGGTTTATTTGAACGGTCACTTGCTCGGTAAACGT 510 0 220 .......................................................................................... 309 2 371 .......................................................................................... 460 P N G Y I S F M Y D M T P Y L K E G N N V L A V R I D H S R Query_1 511 CCGAACGGATACATATCTTTTATGTATGACATGACTCCCTACCTGAAGGAAGGAAATAACGTGCTGGCAGTCCGTATTGACCATAGCCGT 600 0 310 .......................................................................................... 399 2 461 .......................................................................................... 550 Y A D S R W Y T G S G I Y R D V W M I S A P D I H F A Q W G Query_1 601 TATGCCGATTCACGCTGGTACACCGGTTCCGGAATTTACCGGGATGTGTGGATGATTTCCGCCCCGGATATTCATTTTGCTCAATGGGGA 690 0 400 .......................................................................................... 489 2 551 .......................................................................................... 640 I G W H A T S L T D K Q A V V A V D M E V E K H V K A A G A Query_1 691 ATAGGCTGGCACGCTACTTCGCTGACCGATAAGCAGGCGGTAGTGGCAGTTGATATGGAAGTCGAAAAACATGTGAAGGCTGCTGGTGCA 780 0 490 .......................................................................................... 579 2 641 .......................................................................................... 730 L E V S A A L Y D A E G A L V A Q G R K K V A D K A E G I A Query_1 781 TTGGAGGTTTCTGCGGCACTTTATGATGCAGAAGGTGCGTTGGTAGCACAAGGCCGTAAGAAAGTGGCAGATAAAGCTGAGGGTATTGCG 870 0 580 .......................................................................................... 669 2 731 .......................................................................................... 820 K E T I S L K V K K P R R W N L D A P Y L Y T L K T E L L Query_1 871 AAAGAAACAATATCACTGAAGGTGAAGAAGCCTCGCCGCTGGAATCTGGATGCACCTTATC-TGTACACGCTGAAGACAGAGTTGCTTTG 959 0 670 .............................................................-............................ 758 2 821 .............................................................T............................ 910 C D G K R I D G S S T N V G L R T L K F D A N K G F A L N G Query_1 960 TGACGGGAAACGGATAGATGGCAGTTCGACGAATGTAGGTCTCCGTACATTGAAGTTTGATGCCAACAAGGGATTTGCCTTGAACGGAAA 1049 0 759 .......................................................................................... 848 2 911 .......................................................-.................................. 999 K W M K V K G V C L H H D A G V L G A V V P P E V W E R R Query_1 1050 GTGGATGAAGGTGAAAGGAGTATGTCTGCA-TCATGA-TGCAGGTGTGCTGGGTGCGGTAG-TTCCTCCTGAAGTATGGGAGCGTCGTCT 1136 0 849 ..............................-......-.......................-............................ 935 0 889 .....-......-..... 874 2 1000 .-N.N.........................-......T.....N.-....-..N.......T.....T...................... 1085 2 1040 .....A......-..... 1024 L R N L K E I G V N A I R M S H N P Q A P V L Y D L C D R Query_1 1137 -GAGAAACCTGAAAG-AGATTGGTGTGAATGCCATCCGTATGAGCCATAATCCGCAGGCACCGGTA-TTGTATGATTTATGTG-ACCGAA 1222 0 936 -..............-..................................................-................-...... 1021 2 1086 G..N..C........A.........NNNT.............-NNN..N........CAC.....NT................A...... 1174 M G F L V M D E A S D E W E F P K R K W I K G W N V G E P S Query_1 1223 TGGGCTTCCTGGTGATGGATGAAGCTTCGGATGAATGGGAATTCCCGAAACGGAAATGGATAAAGGGATGGAATGTCGGTGAACCAAGCT 1312 0 1022 .......................................................................................... 1111 2 1175 .--.NN....-NNN.......-......-.N........N.N.-.N..N..C.... 1224 Y D G T F D F F E E W I E R D V T D M V R R D R N H P C I F Query_1 1313 ATGACGGAACATTTGATTTCTTTGAGGAATGGATTGAACGCGATGTGACGGATATGGTGCGTCGTGACCGGAATCACCCTTGTATATTCT 1402 0 1112 .......................................................................................... 1201 L W S I G N Q V D Y P N D P Y S H P I L D G T T I N Q P M F Query_1 1403 TGTGGAGCATCGGTAATCAGGTAGACTATCCGAACGATCCTTATTCTCACCCTATTTTGGATGGCACAACCATTAATCAGCCTATGTTCG 1492 0 1202 .................G........................................................................ 1291 G G Y K P D A P D A M R I G K I A K R L A A C V R A V D Query_1 1493 GTGGATACAAGCCTGATGCCCCGGATGCCATGCGTAT-CGGT--AAGATTGCGAA-GCGTCTGGCTGCCTGTGTTCGTGCGGTGGA-CAC 1577 0 1292 .....................................-....--...........-..............................-... 1376 1 1159 .......T....TA...........A.N........N.....N.NGTNNG......A... 1100 T S R P V T G A L A G V V M S N Q T E Y P E A V D V V G Y Query_1 1578 TTCACGTCCTGTTACC-GGTGCATTGGCCGGAGT-GGTGATGTCGAATCAGACAGAGTATCCTGAAGCAGTAGATGT-AGTAGGATA-TA 1663 0 1377 ................-.................-..........................................-.........-.. 1462 1 1099 NN-........N....C..N.............NN.......................................N..T.........N.. 1011 N Y T E N R Y D E D H A T Y P D R V I Y G S E T G V G I E Query_1 1664 ATTATACGGAAAACCGTTAT-GACG-AAGATCATGCTACTTATCCTGACCGTGTGATTTACGGTAGTGAAACCGGTGTGGGAATTGAGGC 1751 0 1463 ....................-....-................................................................ 1550 1 1010 ............N.......N....N..............-................................................. 922 A W H A V R D K E F I F G Q F L W T G T D Y L G E S G R W P Query_1 1752 TTGGCATGCAGTACGCGACAAGGAGTTTATTTTCGGACAGTTCCTCTGGACGGGTACAGACTATTTGGGTGAATCAGGCAGATGGCCTTC 1841 0 1551 .......................................................................................... 1640 1 921 .......................................................................................... 832 S R G L Y T G L L D F G S F P K P R G Y F R A S L W S E K P Query_1 1842 CAGAGGTTTGTATACTGGTTTGCTGGACTTTGGCAGCTTCCCTAAACCCCGTGGATACTTCCGTGCTTCGCTTTGGAGTGAAAAACCTGT 1931 0 1641 .......................................................................................... 1730 1 831 ....................-..................................................................... 743 V T Y V G T Y P K H D W V S M D A W D L W N Y E P G Q L I R Query_1 1932 GACTTATGTTGGTACGTATCCTAAGCACGACTGGGTATCAATGGATGCATGGGATTTGTGGAACTACGAACCGGGTCAGTTGATTCGGGT 2021 0 1731 .......................................................................................... 1820 1 742 .......................................................................................... 653 V V C Y T N A P Q A R L L L N G K E V G Q M K P Y D D K S G Query_1 2022 GGTTTGTTATACCAATGCACCGCAGGCTCGTTTGTTGCTGAACGGAAAAGAAGTGGGTCAGATGAAGCCTTACGATGATAAGAGTGGTAT 2111 0 1821 .......................................................................................... 1910 1 652 .......................................................................................... 563 I I Y W D I P Y Q D G E L R A E G C D A T G K V L S S Y A I Query_1 2112 CATTTATTGGGATATTCCTTATCAGGATGGAGAATTGCGTGCAGAAGGTTGTGATGCAACTGGAAAGGTGCTTTCATCTTATGCAATCAA 2201 0 1911 .......................................................................................... 2000 1 562 .......................................................................................... 473 K T S G R P Y A L R A T V D K E V L S P D K A T A H V T I E Query_1 2202 GACCTCAGGACGTCCATACGCCTTGCGGGCTACTGTGGATAAGGAAGTCTTGTCGCCGGATAAGGCTACTGCACATGTGACAATCGAAGT 2291 0 2001 .......................................................................................... 2090 1 472 .......................................................................................... 383 V V D E Q G T I V K L G D N D V T C Q I E G P A R L L G L E Query_1 2292 GGTGGATGAACAAGGTACTATCGTAAAATTAGGCGATAACGACGTAACTTGTCAGATTGAAGGTCCGGCACGCTTGTTGGGACTGGAAGG 2381 0 2091 .......................................................................................... 2180 1 382 .......................................................................................... 293 G S N N G D M S D Y T D N H H R A F H G R L L A Y I Q T T G Query_1 2382 TTCAAATAACGGAGATATGAGTGATTATACAGATAATCACCACCGTGCTTTTCACGGACGTTTGCTGGCTTATATCCAGACTACAGGTGA 2471 0 2181 .......................................................................................... 2270 1 292 .......................................................................................... 203 E K G E I R V K L T S P L L K G T E V V L K A E * H W K W I Query_1 2472 GAAAGGTGAGATTCGTGTGAAACTGACTTCTCCTCTTTTGAAAGGAACGGAAGTGGTATTGAAGGCTGAGTAACATTGGAAGTGGATAAC 2561 0 2271 ...................................................................... 2340 1 202 .......................................................................................... 113 T D P N S S S V D K L A A A L E H H H H H H * D P A A N K A Query_1 2562 GGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCG 2651 1 112 .......................................................................................... 23 R K E Query_1 2652 AAAGGAA 2658 1 22 ...A... 16 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 13009832 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,820 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200125.108 (CMR200125.22-801.P(801).E531Q.pMCSG68)
QVSFGEAKKFNENWLFSLSDDSLSTSVSYDDSKWRKLDLPHDWSVEGQLSPSLASCTGYLPGGIGWYRKHFQVTDKAARHYIYFEGAYNRSEVYLNGHLLGKRPNGYISFMYDMTPYLKEGNNVLAVRIDHSRYADSRWYTGSGIYRDVWMISAPDIHFAQWGIGWHATSLTDKQAVVAVDMEVEKHVKAAGALEVSAALYDAEGALVAQGRKKVADKAEGIAKETISLKVKKPRRWNLDAPYLYTLKTELLCDGKRIDGSSTNVGLRTLKFDANKGFALNGKWMKVKGVCLHHDAGVLGAVVPPEVWERRLRNLKEIGVNAIRMSHNPQAPVLYDLCDRMGFLVMDEASDEWEFPKRKWIKGWNVGEPSYDGTFDFFEEWIERDVTDMVRRDRNHPCIFLWSIGNEVDYPNDPYSHPILDGTTINQPMFGGYKPDAPDAMRIGKIAKRLAACVRAVDTSRPVTGALAGVVMSNQTEYPEAVDVVGYNYTENRYDEDHATYPDRVIYGSETGVGIEAWHAVRDKEFIFGQFLWTGTDYLGESGRWPSRGLYTGLLDFGSFPKPRGYFRASLWSEKPVTYVGTYPKHDWVSMDAWDLWNYEPGQLIRVVCYTNAPQARLLLNGKEVGQMKPYDDKSGIIYWDIPYQDGELRAEGCDATGKVLSSYAIKTSGRPYALRATVDKEVLSPDKATAHVTIEVVDEQGTIVKLGDNDVTCQIEGPARLLGLEGSNNGDMSDYTDNHHRAFHGRLLAYIQTTGEKGEIRVKLTSPLLKGTEVVLKAE
CAAGTATCTTTTGGCGAAGCCAAAAAATTTAATGAGAATTGGTTGTTCAGTTTGTCGGACGATAGTCTGAGCACTTCCGTATCGTATGACGATAGTAAGTGGCGAAAACTTGATTTGCCGCACGACTGGTCGGTAGAAGGACAGCTTTCTCCTTCCCTGGCAAGCTGTACCGGTTATTTGCCGGGGGGGATAGGCTGGTATCGGAAACATTTTCAGGTTACCGATAAGGCTGCGCGCCACTACATCTATTTTGAGGGAGCATATAACCGCAGCGAGGTTTATTTGAACGGTCACTTGCTCGGTAAACGTCCGAACGGATACATATCTTTTATGTATGACATGACTCCCTACCTGAAGGAAGGAAATAACGTGCTGGCAGTCCGTATTGACCATAGCCGTTATGCCGATTCACGCTGGTACACCGGTTCCGGAATTTACCGGGATGTGTGGATGATTTCCGCCCCGGATATTCATTTTGCTCAATGGGGAATAGGCTGGCACGCTACTTCGCTGACCGATAAGCAGGCGGTAGTGGCAGTTGATATGGAAGTCGAAAAACATGTGAAGGCTGCTGGTGCATTGGAGGTTTCTGCGGCACTTTATGATGCAGAAGGTGCGTTGGTAGCACAAGGCCGTAAGAAAGTGGCAGATAAAGCTGAGGGTATTGCGAAAGAAACAATATCACTGAAGGTGAAGAAGCCTCGCCGCTGGAATCTGGATGCACCTTATCTGTACACGCTGAAGACAGAGTTGCTTTGTGACGGGAAACGGATAGATGGCAGTTCGACGAATGTAGGTCTCCGTACATTGAAGTTTGATGCCAACAAGGGATTTGCCTTGAACGGAAAGTGGATGAAGGTGAAAGGAGTATGTCTGCATCATGATGCAGGTGTGCTGGGTGCGGTAGTTCCTCCTGAAGTATGGGAGCGTCGTCTGAGAAACCTGAAAGAGATTGGTGTGAATGCCATCCGTATGAGCCATAATCCGCAGGCACCGGTATTGTATGATTTATGTGACCGAATGGGCTTCCTGGTGATGGATGAAGCTTCGGATGAATGGGAATTCCCGAAACGGAAATGGATAAAGGGATGGAATGTCGGTGAACCAAGCTATGACGGAACATTTGATTTCTTTGAGGAATGGATTGAACGCGATGTGACGGATATGGTGCGTCGTGACCGGAATCACCCTTGTATATTCTTGTGGAGCATCGGTAATGAGGTAGACTATCCGAACGATCCTTATTCTCACCCTATTTTGGATGGCACAACCATTAATCAGCCTATGTTCGGTGGATACAAGCCTGATGCCCCGGATGCCATGCGTATCGGTAAGATTGCGAAGCGTCTGGCTGCCTGTGTTCGTGCGGTGGACACTTCACGTCCTGTTACCGGTGCATTGGCCGGAGTGGTGATGTCGAATCAGACAGAGTATCCTGAAGCAGTAGATGTAGTAGGATATAATTATACGGAAAACCGTTATGACGAAGATCATGCTACTTATCCTGACCGTGTGATTTACGGTAGTGAAACCGGTGTGGGAATTGAGGCTTGGCATGCAGTACGCGACAAGGAGTTTATTTTCGGACAGTTCCTCTGGACGGGTACAGACTATTTGGGTGAATCAGGCAGATGGCCTTCCAGAGGTTTGTATACTGGTTTGCTGGACTTTGGCAGCTTCCCTAAACCCCGTGGATACTTCCGTGCTTCGCTTTGGAGTGAAAAACCTGTGACTTATGTTGGTACGTATCCTAAGCACGACTGGGTATCAATGGATGCATGGGATTTGTGGAACTACGAACCGGGTCAGTTGATTCGGGTGGTTTGTTATACCAATGCACCGCAGGCTCGTTTGTTGCTGAACGGAAAAGAAGTGGGTCAGATGAAGCCTTACGATGATAAGAGTGGTATCATTTATTGGGATATTCCTTATCAGGATGGAGAATTGCGTGCAGAAGGTTGTGATGCAACTGGAAAGGTGCTTTCATCTTATGCAATCAAGACCTCAGGACGTCCATACGCCTTGCGGGCTACTGTGGATAAGGAAGTCTTGTCGCCGGATAAGGCTACTGCACATGTGACAATCGAAGTGGTGGATGAACAAGGTACTATCGTAAAATTAGGCGATAACGACGTAACTTGTCAGATTGAAGGTCCGGCACGCTTGTTGGGACTGGAAGGTTCAAATAACGGAGATATGAGTGATTATACAGATAATCACCACCGTGCTTTTCACGGACGTTTGCTGGCTTATATCCAGACTACAGGTGAGAAAGGTGAGATTCGTGTGAAACTGACTTCTCCTCTTTTGAAAGGAACGGAAGTGGTATTGAAGGCTGAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,891 total letters Query= CMR200125.108 Length=2745 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4212 0.0 1_05B_B8_CMR200125.108_T7term.seq 946 0.0 2_08B_B8_CMR200125.108_T7S.seq 926 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 56 AATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGT 145 2 14 ...............-......................N.NN.G-...N.....G.....G........C.........G..G......G 101 W S H P Q F E K G T E N L Y F Q S N A Q V S F G E A K K F N Query_1 146 GGTCTCATCC-GCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAAGTATCTTTTGGCGAAGCCAAAAAATTTAAT 234 0 1 ................................. 33 2 102 .CN...C.G.T..T..-...TG.....................................NA..........AC..-............N. 189 E N W L F S L S D D S L S T S V S Y D D S K W R K L D L P H Query_1 235 GAGAATTGGTTGTTCAGTTTGTCGGACGATAGTCTGAGCACTTCCGTATCGTATGACGATAGTAAGTGGCGAAAACTTGATTTGCCGCAC 324 0 34 .......................................................................................... 123 2 190 .G......N........NA..A.....TGG..NA.....C...........C........C-.......N.................... 278 D W S V E G Q L S P S L A S C T G Y L P G G I G W Y R K H F Query_1 325 GACTGGTCGGTAGAAGGACAGCTTTCTCCTTCCCTGGCAAGCTGTACCGGTTATTTGCCgggggggATAGGCTGGTATCGGAAACATTTT 414 0 124 .......................................................................................... 213 2 279 ......N...N.C..N.....T..........A...NACC......A..............CC............N...N.....N.... 368 Q V T D K A A R H Y I Y F E G A Y N R S E V Y L N G H L L G Query_1 415 CAGGTTACCGATAAGGCTGCGCGCCACTACATCTATTTTGAGGGAGCATATAACCGCAGCGAGGTTTATTTGAACGGTCACTTGCTCGGT 504 0 214 .......................................................................................... 303 2 369 .NN.........NT......N....TG...N................G...G...T.CTG......A..A......AC.TG.......TG 458 K R P N G Y I S F M Y D M T P Y L K E G N N V L A V R I D H Query_1 505 AAACGTCCGAACGGATACATATCTTTTATGTATGACATGACTCCCTACCTGAAGGAAGGAAATAACGTGCTGGCAGTCCGTATTGACCAT 594 0 304 .......................................................................................... 393 2 459 ....T.A...TG......G.................N..........G....N....A......C.NG.A...NG.N............. 548 S R Y A D S R W Y T G S G I Y R D V W M I S A P D I H F A Q Query_1 595 AGCCGTTATGCCGATTCACGCTGGTACACCGGTTCCGGAATTTACCGGGATGTGTGGATGATTTCCGCCCCGGATATTCATTTTGCTCAA 684 0 394 .......................................................................................... 483 2 549 .C..............AT.A...........N..............T.....A..............A.A.................... 638 W G I G W H A T S L T D K Q A V V A V D M E V E K H V K A A Query_1 685 TGGGGAATAGGCTGGCACGCTACTTCGCTGACCGATAAGCAGGCGGTAGTGGCAGTTGATATGGAAGTCGAAAAACATGTGAAGGCTGCT 774 0 484 .......................................................................................... 573 2 639 ........T......-GT..............TT....N....TNNN.A..AAGA.......C......NC.........A.N..N.... 727 G A L E V S A A L Y D A E G A L V A Q G R K K V A D K A E G Query_1 775 GGTGCATTGGAGGTTTCTGCGGCACTTTATGATGCAGAAGGTGCGTTGGTAGCACAAGGCCGTAAGAAAGTGGCAGATAAAGCTGAGGGT 864 0 574 .......................................................................................... 663 2 728 ..N..C.....N.CC......NNN..G..............G........GNGN...A.G.....N.N.TN..N...CT.C.....C..A 817 I A K E T I S L K V K K P R R W N L D A P Y L Y T L K T E L Query_1 865 ATTGCGAAAGAAACAATATCACTGAAGGTGAAGAAGCCTCGCCGCTGGAATCTGGATGCACCTTATCTGTACACGCTGAAGACAGAGTTG 954 0 664 .......................................................................................... 753 2 818 .C...C.......A............C.....C...N...........N....C........N.......N..N......NGANC..... 907 L C D G K R I D G S S T N V G L R T L K F D A N K G F Query_1 955 CTTTGTG---ACGGGAAACGGATAGATGGC-AGTTCGACGAATGTAGGTCT--CCGTACATTGAAGTTTGATGCCAACAAGGGAT-TTGC 1037 0 754 .......---....................-....................--................................-.... 836 2 908 ....-..NNN..T.A..CT...........T.CN.....N...N..A...NTC...NG.N...C.N......N..NC..N..T..G.... 996 A L N G K W M K V K G V C L H H D A G V L G A V V P P E Query_1 1038 C-TTGAACGG-AAAGTGGATGAAGGT--GAAAGGAGT-ATGTC-TGCATCATGATGCAGGTGTGCTGGGTGCGGTAGTTCCTCCTGAAGT 1121 0 837 .-........-...............--.........-.....-.............................................. 920 0 889 .-............... 874 2 997 NG........C.....-...N..T..NA...C.A...NN....T...N..... 1048 V W E R R L R N L K E I G V N A I R M S H N P Q A P V L Y D Query_1 1122 ATGGGAGCGTCGTCTGAGAAACCTGAAAGAGATTGGTGTGAATGCCATCCGTATGAGCCATAATCCGCAGGCACCGGTATTGTATGATTT 1211 0 921 .......................................................................................... 1010 L C D R M G F L V M D E A S D E W E F P K R K W I K G W N V Query_1 1212 ATGTGACCGAATGGGCTTCCTGGTGATGGATGAAGCTTCGGATGAATGGGAATTCCCGAAACGGAAATGGATAAAGGGATGGAATGTCGG 1301 0 1011 .......................................................................................... 1100 G E P S Y D G T F D F F E E W I E R D V T D M V R R D R N H Query_1 1302 TGAACCAAGCTATGACGGAACATTTGATTTCTTTGAGGAATGGATTGAACGCGATGTGACGGATATGGTGCGTCGTGACCGGAATCACCC 1391 0 1101 .......................................................................................... 1190 P C I F L W S I G N E V D Y P N D P Y S H P I L D G T T I N Query_1 1392 TTGTATATTCTTGTGGAGCATCGGTAATGAGGTAGACTATCCGAACGATCCTTATTCTCACCCTATTTTGGATGGCACAACCATTAATCA 1481 0 1191 .......................................................................................... 1280 Q P M F G G Y K P D A P D A M R I G K I A K R L A A C V R A Query_1 1482 GCCTATGTTCGGTGGATACAAGCCTGATGCCCCGGATGCCATGCGTATCGGTAAGATTGCGAAGCGTCTGGCTGCCTGTGTTCGTGCGGT 1571 0 1281 .......................................................................................... 1370 V D T S R P V T G A L A G V V M S N Q T E Y P E A V D V V G Query_1 1572 GGACACTTCACGTCCTGTTACCGGTGCATTGGCCGGAGTGGTGATGTCGAATCAGACAGAGTATCCTGAAGCAGTAGATGTAGTAGGATA 1661 0 1371 .......................................................................................... 1460 Y N Y T E N R Y D E D H A T Y P D R V I Y G S Q T G V G I E Query_1 1662 TAATTATACGGAAAACCGTTATGACGAAGATCATGCTACTTATCCTGAC-CGTGTGATTTACGGTAGTCAGACCGGTGTGG-GAATTGAG 1749 0 1461 .................................................-..................G.A..........-........ 1548 1 976 ...........C........G...A....G....N...........NN.NN.C...NN... 916 A W H A V R D K E F I F G Q F L W T G T D Y L G E S G R W Query_1 1750 GCTTGGCATGCAGTACGCGACAAG-GAGTTTATTTTCGGACAGTTCCTCTGGACGGGTACAGACTATTTGGGTGAATCAGGCAGATGGCC 1838 0 1549 ........................-................................................................. 1637 1 915 N..C.N........N..T......T...A........A......AAN....T.N.A..G.......G..TNNN.......N......T.T 826 P S R G L Y T G L L D F G S F P K P R G Y F R A S L W S E K Query_1 1839 TTCCAGAGGTTTGTATACTGGTTTGCTGGACTTTGGCAGCTTCCCTAAACCC-CGTGGATACTTCCGTGCTTCGCTTTGGAGTGAAAAAC 1927 0 1638 ....................................................-..................................... 1726 1 825 ..........N...........N...........................T.G......N.N...............A............ 736 P V T Y V G T Y P K H D W V S M D A W D L W N Y E P G Q L Query_1 1928 C-TGTGACTTATGTTGGTACGTATCCTAAGCACGA-CTGGGTATCAATGGATGCATGGGATTTGTGGAACTACGAACCGGGTCAGTTGAT 2015 0 1727 .-.................................-...................................................... 1814 1 735 GG..........A.-..........N......N.NG.GN..............................N.T-......NA....CAN.. 648 I R V V C Y T N A P Q A R L L L N G K E V G Q M K P Y D D K Query_1 2016 TCGGGTGGTTTGTTATACCAATGCACCGCAGGCTCGTTTGTTGCTGAACGGAAAAGAAGTGGGTCAGATGAAGCCTTACGATGATAAGAG 2105 0 1815 .......................................................................................... 1904 1 647 .T.......N......NN.......NG...CAN...NN..............................N...N..N..N........... 558 S G I I Y W D I P Y Q D G E L R A E G C D A T G K V L S S Y Query_1 2106 TGGTATCATTTATTGGGATATTCCTTATCAGGATGGAGAATTGCGTGCAGAAGGTTGTGATGCAACTGGAAAGGTGCTTTCATCTTATGC 2195 0 1905 .......................................................................................... 1994 1 557 ................C................NC.....N.T...N......A..N...CA....C................NCC.N.. 468 A I K T S G R P Y A L R A T V D K E V L S P D K A T A H V T Query_1 2196 AATCAAGACCTCAGGACGTCCATACGCCTTGCGGGCTACTGTGGATAAGGAAGTCTTGTCGCCGGATAAGGCTACTGCACATGTGACAAT 2285 0 1995 .......................................................................................... 2084 1 467 ................G....C.N.A..C.NN...AANNN.A.............CNC.A....N......N.NN.S.N....NTC.... 378 I E V V D E Q G T I V K L G D N D V T C Q I E G P A R L L G Query_1 2286 CGAAGTGGTGGATGAACAAGGTACTATCGTAAAATTAGGCGATAACGACGTAACTTGTCAGATTGAAGGTCCGGCACGCTTGTTGGGACT 2375 0 2085 .......................................................................................... 2174 1 377 G...CA..N......N......GAA..G..C...C....GA...N....C.TN..C....T..N......AA...............CA. 288 L E G S N N G D M S D Y T D N H H R A F H G R L L A Y I Q T Query_1 2376 GGAAGGTTCAAATAACGGAGATATGAGTGATTATACAGATAATCACCACCGTGCTTTTCACGGACGTTTGCTGGCTTATATCCAGACTAC 2465 0 2175 .......................................................................................... 2264 1 287 .......................N.......................-....-.......A.......NC...............-T... 201 T G E K G E I R V K L T S P L L K G T E V V L K A E * H W K Query_1 2466 AGGTGAGAAAGGTGAGATTCGTGTGAAACTGACTTCTCCTCTTTTGAAAGGAACGGAAGTGGTATTGAAGGCTGAGTAACATTGGAAGTG 2555 0 2265 ............................................................................ 2340 1 200 .................CANC.........-..........-.GA...............A...-CC....AG................. 114 W I T D P N S S S V D K L A A A L E H H H H H H * D P A A N Query_1 2556 GATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAA 2645 1 113 .....T.....N....A....TC.T..G.N.C.A.CGA..CAA..............T.....N.....N.N.................. 24 K A R K Query_1 2646 AGCCCGAAAG 2655 1 23 .......... 14 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 13203520 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,891 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200128.104 (CMR200128.23-703.P(703).E418A.pMCSG68)
REVISFNDGWLFKKGPFATDVMKAAAQWESKWTEVTIPHTWNAQDMQVKTNSFYEGAAYYRKTYVIPETMKGKRLFLRFEGVGTCTEVYVNGYLVGTHKGGYSAFVCEIGEQVKYGESNEIVVKADNTARPDVIPVNHSLFGVYGGIYRPVSLVVTEPCHIAVEDYASPGVYITQKNVSKSAAEVNVKVKLDNASLQPETLELENAIYDAEGKLVKKHVRSLELTPQGMQSYTASFQLKRPHLWQGRKDPYLYKVVSRLKQEGRVIDEVVQPLGIRKYEIVAGKGFFLNGEKYPMYGVTRHQDWWGKGSALTNQEHDADLAMIMDIGATTIRLAHYQQAEYMYAKCDSLGLIVWAEIPFVNRVTGQEWDNAHQQMRELVRQNFNHPSIYIWGIHNEVYHPHGYTAALTRSLHDVCKTDDPDRYTAAVNGYGHAEHPVNQNADVQGMNRYFGWYERKIQDIKPWVEGLEKNYPWQKLMLTEYGADANLEHQTEYLGDALNWGKPFYPETFQTKVHEYQWSVIAEHPYIIASYLWNMFDFAVPMWERGGVPARNLKGMVTFDRKIKKDSYYWYKANWSKEPVLYLTQRRNVDRERKETSVTVYSNIGTPVVYLNGKPLEGIRKGYTEVHYVFDKVTLVQGKNILKAVVSYQGKEYVDEIEWNYTGEKNRGTVVHENKKEHAGW
AGAGAGGTAATCAGCTTTAATGATGGCTGGTTGTTTAAAAAAGGGCCGTTTGCAACAGACGTGATGAAGGCAGCTGCCCAGTGGGAGAGTAAATGGACAGAGGTGACCATCCCTCATACCTGGAATGCGCAGGATATGCAGGTGAAGACAAACTCTTTTTATGAGGGAGCGGCTTATTATCGTAAGACTTATGTGATTCCCGAGACCATGAAGGGGAAGCGTTTGTTCTTGCGCTTTGAAGGTGTGGGGACTTGTACGGAAGTGTATGTCAACGGTTATCTGGTGGGTACACACAAGGGAGGATATTCCGCCTTTGTCTGTGAGATTGGTGAGCAGGTAAAATATGGGGAGAGCAATGAGATTGTAGTGAAGGCTGACAATACAGCTCGTCCGGATGTCATTCCGGTGAACCATAGCCTTTTTGGAGTATACGGAGGTATCTATCGCCCGGTGTCGTTGGTAGTGACAGAGCCTTGTCATATTGCGGTGGAAGATTATGCTTCTCCAGGGGTCTATATTACCCAGAAAAATGTGTCGAAAAGTGCAGCGGAAGTAAATGTGAAGGTGAAACTGGACAACGCTTCCTTGCAACCGGAAACACTGGAACTGGAGAATGCAATCTATGATGCGGAAGGCAAACTGGTGAAGAAGCATGTACGCAGTTTGGAACTGACTCCGCAAGGAATGCAGTCGTATACGGCTTCTTTCCAATTAAAACGTCCGCATTTGTGGCAGGGACGGAAAGATCCTTATTTATATAAGGTAGTATCCCGTCTGAAGCAGGAGGGACGGGTGATTGATGAAGTGGTACAGCCGTTGGGTATCCGTAAATATGAAATTGTGGCCGGGAAAGGCTTCTTCCTGAATGGAGAGAAATATCCTATGTATGGAGTAACTCGCCATCAGGACTGGTGGGGAAAAGGTAGTGCGTTGACAAATCAGGAACATGACGCGGATTTAGCCATGATTATGGATATTGGAGCTACTACCATTCGCCTGGCACATTATCAGCAGGCAGAGTATATGTATGCTAAATGCGATAGTCTGGGACTGATAGTCTGGGCTGAGATTCCTTTTGTAAATCGGGTGACGGGGCAGGAATGGGATAATGCCCATCAGCAGATGAGAGAATTGGTTCGACAGAATTTTAATCACCCTTCCATTTATATTTGGGGAATTCATAATGAGGTGTATCATCCGCATGGTTACACAGCTGCATTGACCCGTTCGTTGCATGATGTATGTAAGACGGATGATCCGGACCGTTATACGGCTGCTGTAAACGGGTATGGACATGCTGAACATCCGGTGAACCAGAATGCAGACGTTCAAGGGATGAACCGCTACTTTGGCTGGTATGAAAGGAAGATACAGGATATCAAACCTTGGGTGGAAGGTTTGGAAAAAAATTACCCCTGGCAGAAACTGATGTTGACAGAGTATGGGGCGGATGCGAACTTGGAGCATCAGACTGAATATCTGGGAGATGCCTTGAATTGGGGAAAACCTTTTTATCCTGAGACTTTCCAGACAAAAGTACATGAGTATCAGTGGAGCGTGATTGCTGAACACCCTTACATTATTGCTTCTTACTTGTGGAATATGTTTGATTTTGCTGTGCCTATGTGGGAGCGTGGAGGAGTTCCTGCACGTAACTTGAAGGGAATGGTAACTTTCGACCGTAAGATTAAGAAGGATTCCTATTATTGGTATAAAGCTAACTGGAGTAAGGAACCTGTACTTTATCTGACACAGCGTCGTAATGTGGACAGAGAAAGAAAAGAAACTTCGGTAACCGTGTATTCTAATATCGGAACTCCGGTGGTATATCTGAACGGGAAACCTTTGGAAGGTATCCGTAAAGGATATACAGAGGTGCACTATGTATTTGATAAAGTGACGCTGGTTCAGGGAAAGAATATATTGAAGGCTGTAGTCAGCTATCAGGGAAAAGAGTACGTAGATGAGATAGAATGGAACTATACTGGTGAAAAGAATAGGGGGACTGTCGTGCATGAGAATAAGAAGGAGCATGCAGGATGG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,555 total letters Query= CMR200128.104 Length=2448 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3676 0.0 2_08C_C8_CMR200128.104_T7S.seq 1887 0.0 1_05C_C8_CMR200128.104_T7term.seq 1855 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 60 CCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTC 149 2 20 ...........-.............................................................................. 108 S H P Q F E K G T E N L Y F Q S N A R E V I S F N D G W L F Query_1 150 TCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCAGAGAGGTAATCAGCTTTAATGATGGCTGGTTGTTTAA 239 0 1 ...................................... 38 0 1732 ............ 1743 2 109 .......................................................................................... 198 1 441 ............ 430 K K G P F A T D V M K A A A Q W E S K W T E V T I P H T W N Query_1 240 AAAAGGGCCGTTTGCAACAGACGTGATGAAGGCAGCTGCCCAGTGGGAGAGTAAATGGACAGAGGTGACCATCCCTCATACCTGGAATGC 329 0 39 .......................................................................................... 128 2 199 .......................................................................................... 288 A Q D M Q V K T N S F Y E G A A Y Y R K T Y V I P E T M K G Query_1 330 GCAGGATATGCAGGTGAAGACAAACTCTTTTTATGAGGGAGCGGCTTATTATCGTAAGACTTATGTGATTCCCGAGACCATGAAGGGGAA 419 0 129 .......................................................................................... 218 2 289 .......................................................................................... 378 K R L F L R F E G V G T C T E V Y V N G Y L V G T H K G G Y Query_1 420 GCGTTTGTTCTTGCGCTTTGAAGGTGTGGGGACTTGTACGGAAGTGTATGTCAACGGTTATCTGGTGGGTACACACAAGGGAGGATATTC 509 0 219 .......................................................................................... 308 2 379 .......................................................................................... 468 S A F V C E I G E Q V K Y G E S N E I V V K A D N T A R P D Query_1 510 CGCCTTTGTCTGTGAGATTGGTGAGCAGGTAAAATATGGGGAGAGCAATGAGATTGTAGTGAAGGCTGACAATACAGCTCGTCCGGATGT 599 0 309 .......................................................................................... 398 2 469 .......................................................................................... 558 V I P V N H S L F G V Y G G I Y R P V S L V V T E P C H I A Query_1 600 CATTCCGGTGAACCATAGCCTTTTTGGAGTATACGGAGGTATCTATCGCCCGGTGTCGTTGGTAGTGACAGAGCCTTGTCATATTGCGGT 689 0 399 .......................................................................................... 488 0 1305 ............ 1316 2 559 .......................................................................................... 648 V E D Y A S P G V Y I T Q K N V S K S A A E V N V K V K L D Query_1 690 GGAAGATTATGCTTCTCCAGGGGTCTATATTACCCAGAAAAATGTGTCGAAAAGTGCAGCGGAAGTAAATGTGAAGGTGAAACTGGACAA 779 0 489 .......................................................................................... 578 2 649 .......................................................................................... 738 N A S L Q P E T L E L E N A I Y D A E G K L V K K H V R S L Query_1 780 CGCTTCCTTGCAACCGGAAACACTGGAACTGGAGAATGCAATCTATGATGCGGAAGGCAAACTGGTGAAGAAGCATGTACGCAGTTTGGA 869 0 579 .......................................................................................... 668 2 739 .......................................................................................N.. 828 E L T P Q G M Q S Y T A S F Q L K R P H L W Q G R K D P Y Query_1 870 ACTGACTCCGCAA-GGAATGCAGTCGTATACGGCTTCTTTCCAA-TTAAAACGTCCGCATTTGTGGCAGGGACGGAAAGATCC-TTATTT 956 0 669 .............-..............................-......................................-...... 755 2 829 .............N..............................T......................................T...... 918 L Y K V V S R L K Q E G R V I D E V V Q P L G I R K Y E I V Query_1 957 ATATAAGGTAGTATCCCGTCTGAAGCAGGAGGGACGGGTGATTGATGAAGTGGTACAGCCGTTGGGTATCCGTAAATATGAAATTGTGGC 1046 0 756 .......................................................................................... 845 0 1852 ............ 1863 2 919 .............................................................-.N.........-................ 1006 1 321 ............ 310 A G K G F F L N G E K Y P M Y G V T R H Q D W W G K G S Query_1 1047 CGGGAAAGGCTTCTTCCTGAATGGAGAGAAATATCCTATGT-ATGGAGTAACTCGCCA-TCAGG-ACTGGTGGGGAAAA-GGTAG-TGC- 1130 0 846 .........................................-................-.....-..............-.....-...- 929 2 1007 .........................................NN...............T.....A..........N...G.....N...G 1096 2 1072 ...........C... 1058 A L T N Q E H D A D L A M I M D I G A T T I R L A H Y Q Query_1 1131 GTTGACAAATC-AGGAACAT-GACGCGGATTTAGCCATG-ATTAT-GGATATTGG-AGCTACTACCATT-CGCCTGGCAC-ATTATCAGC 1213 0 930 ...........-........-..................-.....-.........-.............-..........-......... 1012 2 1097 .C...NC..N.A........N...N...CA.NNNN....A.....G...NNN...A.........N...C.N....NNN.-......... 1185 1 1189 .......CN........ 1173 Q A E Y M Y A K C D S L G L I V W A E I P F V N R V T Query_1 1214 AGGCAGA--G-TATAT-GTATGCTAAATGCG---ATAGTCTGGGACTGATA-GTCTGGGCTGAGATTCCTTTTGTAAATCGGGTGAC-GG 1294 0 1013 .......--.-.....-..............---.................-...................................-.. 1093 2 1186 ..NNN..NNN-..N..-........ 1208 1 1172 .N.....--.A.N..NN.....NCT......CAAN....TNT.........A..NNN..N...ANA.N.N.NG...G-.........C.. 1086 G Q E W D N A H Q Q M R E L V R Q N F N H P S I Y I W G I Query_1 1295 GGCAGGAATGGGATAATGCCCATCAGC-AGATGAGAG-AATTGGTTCGACAGAATTTTAA--TCACCCTTCCATTTATATTTGGGGAATT 1380 0 1094 ...........................-.........-......................--............................ 1179 1 1085 ........AT.................C.........G......................ATNNN..T............N....-.... 997 H N A V Y H P H G Y T A A L T R S L H D V C K T D D P D R Query_1 1381 CATAATGCTGTGTATCAT-CCGCATGGTTACACAGCTGCATTGACCCGTTCGTTGCATGATGTATGTAAGACGGATGATCCGGACCGTTA 1469 0 1180 .......AG.........-....................................................................... 1268 1 996 ..................T....................................................................... 907 Y T A A V N G Y G H A E H P V N Q N A D V Q G M N R Y F G W Query_1 1470 TACGGCTGCTGTAAACGGGTATGGACATGCTGAACATCCGGTGAA-CCAGAATGCAGACGTTCAAGGGATGAACCGCTACTTTGGCTGGT 1558 0 1269 .............................................-............................................ 1357 0 402 .........-... 413 2 562 .........-... 573 1 906 .................................G...........A............................................ 817 Y E R K I Q D I K P W V E G L E K N Y P W Q K L M L T E Y G Query_1 1559 ATGAAAGGAAGATACAGGATATCAAACCTTGGGTGGAAGGTTTGGaaaaaaa-TTACCCCTGGCAGAAACTGATGTTGACAGAGTATGGG 1647 0 1358 ....................................................-..................................... 1446 1 816 ....................................................A..................................... 727 A D A N L E H Q T E Y L G D A L N W G K P F Y P E T F Q T K Query_1 1648 GCGGATGCGAACTTGGAGCATCAGACTGAATATCTGGGAGATGCCTTGAATTGGGGAAAACCTTTTTATCCTGAGACTTTCCAGACAAAA 1737 0 1447 .......................................................................................... 1536 1 726 .......................................................................................... 637 V H E Y Q W S V I A E H P Y I I A S Y L W N M F D F A V P M Query_1 1738 GTACATGAGTATCAGTGGAGCGTGATTGCTGAACACCCTTACATTATTGCTTCTTACTTGTGGAATATGTTTGATTTTGCTGTGCCTATG 1827 0 1537 .......................................................................................... 1626 1 636 .......................................................................................... 547 W E R G G V P A R N L K G M V T F D R K I K K D S Y Y W Y K Query_1 1828 TGGGAGCGTGGAGGAGTTCCTGCACGTAACTTGAAGGGAATGGTAACTTTCGACCGTAAGATTAAGAAGGATTCCTATTATTGGTATAAA 1917 0 1627 .......................................................................................... 1716 1 546 .......................................................................................... 457 A N W S K E P V L Y L T Q R R N V D R E R K E T S V T V Y S Query_1 1918 GCTAACTGGAGTAAGGAACCTGTACTTTATCTGACACAGCGTCGTAATGTGGACAGAGAAAGAAAAGAAACTTCGGTAACCGTGTATTCT 2007 0 1717 .......................................................................................... 1806 2 136 ............ 147 1 456 .......................................................................................... 367 N I G T P V V Y L N G K P L E G I R K G Y T E V H Y V F D K Query_1 2008 AATATCGGAACTCCGGTGGTATATCTGAACGGGAAACCTTTGGAAGGTATCCGTAAAGGATATACAGAGGTGCACTATGTATTTGATAAA 2097 0 1807 .......................................................................................... 1896 0 820 ............ 831 2 982 ........... 992 1 366 .......................................................................................... 277 V T L V Q G K N I L K A V V S Y Q G K E Y V D E I E W N Y T Query_1 2098 GTGACGCTGGTTCAGGGAAAGAATATATTGAAGGCTGTAGTCAGCTATCAGGGAAAAGAGTACGTAGATGAGATAGAATGGAACTATACT 2187 0 1897 .......................................................................................... 1986 1 276 .......................................................................................... 187 G E K N R G T V V H E N K K E H A G W * H W K W I T D P N S Query_1 2188 GGTGAAAAGAATAGGGGGACTGTCGTGCATGAGAATAAGAAGGAGCATGCAGGATGGTAACATTGGAAGTGGATAACGGATCCGAATTCG 2277 0 1987 ......................................................... 2043 1 186 .......................................................................................... 97 S S V D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 2278 AGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAG-CCCGAAAGGAAG 2362 1 96 .................................---.....................................C.......A.... 14 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 10949224 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,555 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200128.105 (CMR200128.23-703.P(703).E502A.pMCSG68)
REVISFNDGWLFKKGPFATDVMKAAAQWESKWTEVTIPHTWNAQDMQVKTNSFYEGAAYYRKTYVIPETMKGKRLFLRFEGVGTCTEVYVNGYLVGTHKGGYSAFVCEIGEQVKYGESNEIVVKADNTARPDVIPVNHSLFGVYGGIYRPVSLVVTEPCHIAVEDYASPGVYITQKNVSKSAAEVNVKVKLDNASLQPETLELENAIYDAEGKLVKKHVRSLELTPQGMQSYTASFQLKRPHLWQGRKDPYLYKVVSRLKQEGRVIDEVVQPLGIRKYEIVAGKGFFLNGEKYPMYGVTRHQDWWGKGSALTNQEHDADLAMIMDIGATTIRLAHYQQAEYMYAKCDSLGLIVWAEIPFVNRVTGQEWDNAHQQMRELVRQNFNHPSIYIWGIHNEVYHPHGYTAALTRSLHDVCKTDDPDRYTAAVNGYGHAEHPVNQNADVQGMNRYFGWYERKIQDIKPWVEGLEKNYPWQKLMLTEYGADANLEHQTEYLGDALNWGKPFYPETFQTKVHEYQWSVIAEHPYIIASYLWNMFDFAVPMWERGGVPARNLKGMVTFDRKIKKDSYYWYKANWSKEPVLYLTQRRNVDRERKETSVTVYSNIGTPVVYLNGKPLEGIRKGYTEVHYVFDKVTLVQGKNILKAVVSYQGKEYVDEIEWNYTGEKNRGTVVHENKKEHAGW
AGAGAGGTAATCAGCTTTAATGATGGCTGGTTGTTTAAAAAAGGGCCGTTTGCAACAGACGTGATGAAGGCAGCTGCCCAGTGGGAGAGTAAATGGACAGAGGTGACCATCCCTCATACCTGGAATGCGCAGGATATGCAGGTGAAGACAAACTCTTTTTATGAGGGAGCGGCTTATTATCGTAAGACTTATGTGATTCCCGAGACCATGAAGGGGAAGCGTTTGTTCTTGCGCTTTGAAGGTGTGGGGACTTGTACGGAAGTGTATGTCAACGGTTATCTGGTGGGTACACACAAGGGAGGATATTCCGCCTTTGTCTGTGAGATTGGTGAGCAGGTAAAATATGGGGAGAGCAATGAGATTGTAGTGAAGGCTGACAATACAGCTCGTCCGGATGTCATTCCGGTGAACCATAGCCTTTTTGGAGTATACGGAGGTATCTATCGCCCGGTGTCGTTGGTAGTGACAGAGCCTTGTCATATTGCGGTGGAAGATTATGCTTCTCCAGGGGTCTATATTACCCAGAAAAATGTGTCGAAAAGTGCAGCGGAAGTAAATGTGAAGGTGAAACTGGACAACGCTTCCTTGCAACCGGAAACACTGGAACTGGAGAATGCAATCTATGATGCGGAAGGCAAACTGGTGAAGAAGCATGTACGCAGTTTGGAACTGACTCCGCAAGGAATGCAGTCGTATACGGCTTCTTTCCAATTAAAACGTCCGCATTTGTGGCAGGGACGGAAAGATCCTTATTTATATAAGGTAGTATCCCGTCTGAAGCAGGAGGGACGGGTGATTGATGAAGTGGTACAGCCGTTGGGTATCCGTAAATATGAAATTGTGGCCGGGAAAGGCTTCTTCCTGAATGGAGAGAAATATCCTATGTATGGAGTAACTCGCCATCAGGACTGGTGGGGAAAAGGTAGTGCGTTGACAAATCAGGAACATGACGCGGATTTAGCCATGATTATGGATATTGGAGCTACTACCATTCGCCTGGCACATTATCAGCAGGCAGAGTATATGTATGCTAAATGCGATAGTCTGGGACTGATAGTCTGGGCTGAGATTCCTTTTGTAAATCGGGTGACGGGGCAGGAATGGGATAATGCCCATCAGCAGATGAGAGAATTGGTTCGACAGAATTTTAATCACCCTTCCATTTATATTTGGGGAATTCATAATGAGGTGTATCATCCGCATGGTTACACAGCTGCATTGACCCGTTCGTTGCATGATGTATGTAAGACGGATGATCCGGACCGTTATACGGCTGCTGTAAACGGGTATGGACATGCTGAACATCCGGTGAACCAGAATGCAGACGTTCAAGGGATGAACCGCTACTTTGGCTGGTATGAAAGGAAGATACAGGATATCAAACCTTGGGTGGAAGGTTTGGAAAAAAATTACCCCTGGCAGAAACTGATGTTGACAGAGTATGGGGCGGATGCGAACTTGGAGCATCAGACTGAATATCTGGGAGATGCCTTGAATTGGGGAAAACCTTTTTATCCTGAGACTTTCCAGACAAAAGTACATGAGTATCAGTGGAGCGTGATTGCTGAACACCCTTACATTATTGCTTCTTACTTGTGGAATATGTTTGATTTTGCTGTGCCTATGTGGGAGCGTGGAGGAGTTCCTGCACGTAACTTGAAGGGAATGGTAACTTTCGACCGTAAGATTAAGAAGGATTCCTATTATTGGTATAAAGCTAACTGGAGTAAGGAACCTGTACTTTATCTGACACAGCGTCGTAATGTGGACAGAGAAAGAAAAGAAACTTCGGTAACCGTGTATTCTAATATCGGAACTCCGGTGGTATATCTGAACGGGAAACCTTTGGAAGGTATCCGTAAAGGATATACAGAGGTGCACTATGTATTTGATAAAGTGACGCTGGTTCAGGGAAAGAATATATTGAAGGCTGTAGTCAGCTATCAGGGAAAAGAGTACGTAGATGAGATAGAATGGAACTATACTGGTGAAAAGAATAGGGGGACTGTCGTGCATGAGAATAAGAAGGAGCATGCAGGATGG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,616 total letters Query= CMR200128.105 Length=2448 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3676 0.0 2_08D_D8_CMR200128.105_T7S.seq 1833 0.0 1_05D_D8_CMR200128.105_T7term.seq 1700 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 58 TTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGG 147 2 19 .......................................................................................... 108 S H P Q F E K G T E N L Y F Q S N A R E V I S F N D G W L F Query_1 148 TCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCAGAGAGGTAATCAGCTTTAATGATGGCTGGTTGTTT 237 0 1 .................................... 36 0 1732 ............ 1743 2 109 .......................................................................................... 198 1 440 ............ 429 K K G P F A T D V M K A A A Q W E S K W T E V T I P H T W N Query_1 238 AAAAAAGGGCCGTTTGCAACAGACGTGATGAAGGCAGCTGCCCAGTGGGAGAGTAAATGGACAGAGGTGACCATCCCTCATACCTGGAAT 327 0 37 .......................................................................................... 126 2 199 .......................................................................................... 288 A Q D M Q V K T N S F Y E G A A Y Y R K T Y V I P E T M K G Query_1 328 GCGCAGGATATGCAGGTGAAGACAAACTCTTTTTATGAGGGAGCGGCTTATTATCGTAAGACTTATGTGATTCCCGAGACCATGAAGGGG 417 0 127 .......................................................................................... 216 2 289 .......................................................................................... 378 K R L F L R F E G V G T C T E V Y V N G Y L V G T H K G G Y Query_1 418 AAGCGTTTGTTCTTGCGCTTTGAAGGTGTGGGGACTTGTACGGAAGTGTATGTCAACGGTTATCTGGTGGGTACACACAAGGGAGGATAT 507 0 217 .......................................................................................... 306 2 379 .......................................................................................... 468 S A F V C E I G E Q V K Y G E S N E I V V K A D N T A R P D Query_1 508 TCCGCCTTTGTCTGTGAGATTGGTGAGCAGGTAAAATATGGGGAGAGCAATGAGATTGTAGTGAAGGCTGACAATACAGCTCGTCCGGAT 597 0 307 .......................................................................................... 396 2 469 .......................................................................................... 558 V I P V N H S L F G V Y G G I Y R P V S L V V T E P C H I A Query_1 598 GTCATTCCGGTGAACCATAGCCTTTTTGGAGTATACGGAGGTATCTATCGCCCGGTGTCGTTGGTAGTGACAGAGCCTTGTCATATTGCG 687 0 397 .......................................................................................... 486 0 1305 ............ 1316 2 559 .......................................................................................... 648 V E D Y A S P G V Y I T Q K N V S K S A A E V N V K V K L D Query_1 688 GTGGAAGATTATGCTTCTCCAGGGGTCTATATTACCCAGAAAAATGTGTCGAAAAGTGCAGCGGAAGTAAATGTGAAGGTGAAACTGGAC 777 0 487 .......................................................................................... 576 2 649 .......................................................................................... 738 N A S L Q P E T L E L E N A I Y D A E G K L V K K H V R S L Query_1 778 AACGCTTCCTTGCAACCGGAAACACTGGAACTGGAGAATGCAATCTATGATGCGGAAGGCAAACTGGTGAAGAAGCATGTACGCAGTTTG 867 0 577 .......................................................................................... 666 2 739 .......................................................................................... 828 E L T P Q G M Q S Y T A S F Q L K R P H L W Q G R K D P Y Query_1 868 GAACTGACTCCGCAAGGAATGCAGTCGTATACGGCTTCTTTCCAATTAAAACGTCCGCATTT-GTGGCAGGGACGGAAAGAT-CCTTA-T 954 0 667 ..............................................................-...................-.....-. 753 2 829 ............................................N.-...............T......-............N.....T. 916 2 1018 ........... 1028 L Y K V V S R L K Q E G R V I D E V V Q P L G I R K Y E I Query_1 955 TTATATAAGGTAGTATCCCGTCTGAAGCAGGAGGGACGGGTGATTGATGAAGTGGTACAGCCGTTGGGTA-TCCGTAAATATGAAATTGT 1043 0 754 ......................................................................-................... 842 0 1852 ....-........ 1863 2 917 ..........A.................................................N.........T....N.............. 1006 1 320 ....-........ 309 V A G K G F F L N G E K Y P M Y G V T R H Q D W W G K G Query_1 1044 GGCCGGG-AAAGGCTTC-TTCCTGAATGGAGAGAAA-TATCCTATGTA-TGGAGTAAC-TCG-CCATCAGGA-CTGGTGGGGAAAA-GGT 1125 0 843 .......-.........-..................-...........-.........-...-.........-.............-... 924 2 1007 .......A.........T..................N...........N.........N...T.NT......NN............G... 1096 S A L T N Q E H D A D L A M I M D I G A T T I R L A Query_1 1126 AGT-GCGTTGACAAA-TCAGGAACA--TGACGC-GGATTTAGC-CAT-GATTATGGA-TA-TTGGAGCTACTACC--ATTCGCCTGGC-A 1203 0 925 ...-...........-.........--......-.........-...-.........-..-..............--...........-. 1002 2 1097 ...N.....NN....T.....G.NNNTN.....N...A..NN.N...N..NN.....N..T.......N......CAT.....N..N.N. 1186 H Y Q Q A E Y M Y A K C D S L G L I V W A E I P F V N R V T Query_1 1204 CATTATCAGCAGGCAGAGTATATGTATGCTAAATGCGATAGTCTGGGACTGATAGTCTGGGCTGAGATTCCTTTTGTAAATCGGGTGACG 1293 0 1003 .......................................................................................... 1092 2 1187 ..... 1191 1 1109 ........ 1102 G Q E W D N A H Q Q M R E L V R Q N F N H P S I Y I Query_1 1294 GGG--CAGGAATGGGA--TAAT-GCCCATCAG-CAGAT-GAGA-GAATTGGTTCGACAGAATTTTAATCACCCTT-CCATTTA-TATTTG 1373 0 1093 ...--...........--....-.........-.....-....-...............................-.......-...... 1172 1 1101 ...GCN......T...GA....TNNNN.....G...NNN.N..N.N............NG...............T.......A...... 1012 W G I H N E V Y H P H G Y T A A L T R S L H D V C K T D D Query_1 1374 GGGAATTCATAATGAGGTGTAT-CAT-CCGCA-TGGTTACACAGCTGCA-TTGACCCGTTCGTTGCATGATGTATGTAAGACGGATGATC 1459 0 1173 ......................-...-.....-................-........................................ 1258 1 1011 ......................T...T.....A................A.............G........................AT 922 P D R Y T A A V N G Y G H A E H P V N Q N A D V Q G M N R Query_1 1460 CGGACCGTTATACGGCTGCTGT-AAACGGGTATGGACATGCTGAACATCCGG-TGAACCAGAATGCAGACGTTCAAGGG-ATGAACCGCT 1546 0 1259 ......................-.............................-..........................-.......... 1345 0 402 .....-....... 413 2 564 .....-....... 575 1 921 .C.N..................T..........N..........G.......G..........N...............G.......... 832 Y F G W Y E R K I Q D I K P W V E G L E K N Y P W Q K L M Query_1 1547 ACTTTGGCTGGTATGAAA-GGAAGATACAGGATATCAAACCTTGGGTGGAA-GGTTTGGaaaaaaaTTACCCCTGGCAGAAACTGATGTT 1634 0 1346 ..................-................................-...................................... 1433 1 831 .........N........A................................A...................................... 742 L T A Y G A D A N L E H Q T E Y L G D A L N W G K P F Y P E Query_1 1635 GACAGCTTATGGGGCGGATGCGAACTTGGAGCATCAGACTGAATATCTGGGAGATGCCTTGAA-TTGGGGAAAA-CCTTTTTATCCTGAG 1722 0 1434 .....AG........................................................-..........-............... 1521 1 741 ...............................................................A..........A............... 652 T F Q T K V H E Y Q W S V I A E H P Y I I A S Y L W N M F Query_1 1723 ACTTTCCAGACAAAA-GTACATGAGTATCAGTGGAGCGTGATTGCTGAACACCCTTACATTATTGCTTCTTACTTGTGGAATATGTTTGA 1811 0 1522 ...............-.......................................................................... 1610 1 651 ...............A.......................................................................... 562 D F A V P M W E R G G V P A R N L K G M V T F D R K I K K D Query_1 1812 TTTTGCTGTGCCTATGTGGGAGCGTGGAGGAGTTCCTGCACGTAACTTGAAGGGAATGGTAACTTTCGACCGTAAGATTAAGAAGGATTC 1901 0 1611 .......................................................................................... 1700 1 561 .......................................................................................... 472 S Y Y W Y K A N W S K E P V L Y L T Q R R N V D R E R K E T Query_1 1902 CTATTATTGGTATAAAGCTAACTGGAGTAAGGAACCTGTACTTTATCTGACACAGCGTCGTAATGTGGACAGAGAAAGAAAAGAAACTTC 1991 0 1701 .......................................................................................... 1790 2 138 ............ 149 1 471 .......................................................................................... 382 S V T V Y S N I G T P V V Y L N G K P L E G I R K G Y T E V Query_1 1992 GGTAACCGTGTATTCTAATATCGGAACTCCGGTGGTATATCTGAACGGGAAACCTTTGGAAGGTATCCGTAAAGGATATACAGAGGTGCA 2081 0 1791 .......................................................................................... 1880 0 820 ............ 831 1 381 .......................................................................................... 292 H Y V F D K V T L V Q G K N I L K A V V S Y Q G K E Y V D E Query_1 2082 CTATGTATTTGATAAAGTGACGCTGGTTCAGGGAAAGAATATATTGAAGGCTGTAGTCAGCTATCAGGGAAAAGAGTACGTAGATGAGAT 2171 0 1881 .......................................................................................... 1970 1 291 .......................................................................................... 202 I E W N Y T G E K N R G T V V H E N K K E H A G W * H W K W Query_1 2172 AGAATGGAACTATACTGGTGAAAAGAATAGGGGGACTGTCGTGCATGAGAATAAGAAGGAGCATGCAGGATGGTAACATTGGAAGTGGAT 2261 0 1971 ......................................................................... 2043 1 201 .......................................................................................... 112 I T D P N S S S V D K L A A A L E H H H H H H * D P A A N K Query_1 2262 AACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGC 2351 1 111 ...............................NN................---...................................... 25 A R Query_1 2352 CCGAAA 2357 1 24 ...... 19 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11097515 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,616 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200128.106 (CMR200128.23-703.P(703).E418Q.pMCSG68)
REVISFNDGWLFKKGPFATDVMKAAAQWESKWTEVTIPHTWNAQDMQVKTNSFYEGAAYYRKTYVIPETMKGKRLFLRFEGVGTCTEVYVNGYLVGTHKGGYSAFVCEIGEQVKYGESNEIVVKADNTARPDVIPVNHSLFGVYGGIYRPVSLVVTEPCHIAVEDYASPGVYITQKNVSKSAAEVNVKVKLDNASLQPETLELENAIYDAEGKLVKKHVRSLELTPQGMQSYTASFQLKRPHLWQGRKDPYLYKVVSRLKQEGRVIDEVVQPLGIRKYEIVAGKGFFLNGEKYPMYGVTRHQDWWGKGSALTNQEHDADLAMIMDIGATTIRLAHYQQAEYMYAKCDSLGLIVWAEIPFVNRVTGQEWDNAHQQMRELVRQNFNHPSIYIWGIHNEVYHPHGYTAALTRSLHDVCKTDDPDRYTAAVNGYGHAEHPVNQNADVQGMNRYFGWYERKIQDIKPWVEGLEKNYPWQKLMLTEYGADANLEHQTEYLGDALNWGKPFYPETFQTKVHEYQWSVIAEHPYIIASYLWNMFDFAVPMWERGGVPARNLKGMVTFDRKIKKDSYYWYKANWSKEPVLYLTQRRNVDRERKETSVTVYSNIGTPVVYLNGKPLEGIRKGYTEVHYVFDKVTLVQGKNILKAVVSYQGKEYVDEIEWNYTGEKNRGTVVHENKKEHAGW
AGAGAGGTAATCAGCTTTAATGATGGCTGGTTGTTTAAAAAAGGGCCGTTTGCAACAGACGTGATGAAGGCAGCTGCCCAGTGGGAGAGTAAATGGACAGAGGTGACCATCCCTCATACCTGGAATGCGCAGGATATGCAGGTGAAGACAAACTCTTTTTATGAGGGAGCGGCTTATTATCGTAAGACTTATGTGATTCCCGAGACCATGAAGGGGAAGCGTTTGTTCTTGCGCTTTGAAGGTGTGGGGACTTGTACGGAAGTGTATGTCAACGGTTATCTGGTGGGTACACACAAGGGAGGATATTCCGCCTTTGTCTGTGAGATTGGTGAGCAGGTAAAATATGGGGAGAGCAATGAGATTGTAGTGAAGGCTGACAATACAGCTCGTCCGGATGTCATTCCGGTGAACCATAGCCTTTTTGGAGTATACGGAGGTATCTATCGCCCGGTGTCGTTGGTAGTGACAGAGCCTTGTCATATTGCGGTGGAAGATTATGCTTCTCCAGGGGTCTATATTACCCAGAAAAATGTGTCGAAAAGTGCAGCGGAAGTAAATGTGAAGGTGAAACTGGACAACGCTTCCTTGCAACCGGAAACACTGGAACTGGAGAATGCAATCTATGATGCGGAAGGCAAACTGGTGAAGAAGCATGTACGCAGTTTGGAACTGACTCCGCAAGGAATGCAGTCGTATACGGCTTCTTTCCAATTAAAACGTCCGCATTTGTGGCAGGGACGGAAAGATCCTTATTTATATAAGGTAGTATCCCGTCTGAAGCAGGAGGGACGGGTGATTGATGAAGTGGTACAGCCGTTGGGTATCCGTAAATATGAAATTGTGGCCGGGAAAGGCTTCTTCCTGAATGGAGAGAAATATCCTATGTATGGAGTAACTCGCCATCAGGACTGGTGGGGAAAAGGTAGTGCGTTGACAAATCAGGAACATGACGCGGATTTAGCCATGATTATGGATATTGGAGCTACTACCATTCGCCTGGCACATTATCAGCAGGCAGAGTATATGTATGCTAAATGCGATAGTCTGGGACTGATAGTCTGGGCTGAGATTCCTTTTGTAAATCGGGTGACGGGGCAGGAATGGGATAATGCCCATCAGCAGATGAGAGAATTGGTTCGACAGAATTTTAATCACCCTTCCATTTATATTTGGGGAATTCATAATGAGGTGTATCATCCGCATGGTTACACAGCTGCATTGACCCGTTCGTTGCATGATGTATGTAAGACGGATGATCCGGACCGTTATACGGCTGCTGTAAACGGGTATGGACATGCTGAACATCCGGTGAACCAGAATGCAGACGTTCAAGGGATGAACCGCTACTTTGGCTGGTATGAAAGGAAGATACAGGATATCAAACCTTGGGTGGAAGGTTTGGAAAAAAATTACCCCTGGCAGAAACTGATGTTGACAGAGTATGGGGCGGATGCGAACTTGGAGCATCAGACTGAATATCTGGGAGATGCCTTGAATTGGGGAAAACCTTTTTATCCTGAGACTTTCCAGACAAAAGTACATGAGTATCAGTGGAGCGTGATTGCTGAACACCCTTACATTATTGCTTCTTACTTGTGGAATATGTTTGATTTTGCTGTGCCTATGTGGGAGCGTGGAGGAGTTCCTGCACGTAACTTGAAGGGAATGGTAACTTTCGACCGTAAGATTAAGAAGGATTCCTATTATTGGTATAAAGCTAACTGGAGTAAGGAACCTGTACTTTATCTGACACAGCGTCGTAATGTGGACAGAGAAAGAAAAGAAACTTCGGTAACCGTGTATTCTAATATCGGAACTCCGGTGGTATATCTGAACGGGAAACCTTTGGAAGGTATCCGTAAAGGATATACAGAGGTGCACTATGTATTTGATAAAGTGACGCTGGTTCAGGGAAAGAATATATTGAAGGCTGTAGTCAGCTATCAGGGAAAAGAGTACGTAGATGAGATAGAATGGAACTATACTGGTGAAAAGAATAGGGGGACTGTCGTGCATGAGAATAAGAAGGAGCATGCAGGATGG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,303 total letters Query= CMR200128.106 Length=2448 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3680 0.0 1_05E_E8_CMR200128.106_T7term.seq 1721 0.0 2_08E_E8_CMR200128.106_T7S.seq 1709 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 60 CCCCTCTAGAAATAATTTT-GTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGT 148 2 21 ...N...N...N.......T...................................................................... 110 S H P Q F E K G T E N L Y F Q S N A R E V I S F N D G W L F Query_1 149 CTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCAGAGAGGTAATCAGCTTTAATGATGGCTGGTTGTTTA 238 0 1 ..................................... 37 0 1732 ............ 1743 1 439 ............ 428 2 111 .......................................................................................... 200 K K G P F A T D V M K A A A Q W E S K W T E V T I P H T W N Query_1 239 AAAAAGGGCCGTTTGCAACAGACGTGATGAAGGCAGCTGCCCAGTGGGAGAGTAAATGGACAGAGGTGACCATCCCTCATACCTGGAATG 328 0 38 .......................................................................................... 127 2 201 .......................................................................................... 290 A Q D M Q V K T N S F Y E G A A Y Y R K T Y V I P E T M K G Query_1 329 CGCAGGATATGCAGGTGAAGACAAACTCTTTTTATGAGGGAGCGGCTTATTATCGTAAGACTTATGTGATTCCCGAGACCATGAAGGGGA 418 0 128 .......................................................................................... 217 2 291 .......................................................................................... 380 K R L F L R F E G V G T C T E V Y V N G Y L V G T H K G G Y Query_1 419 AGCGTTTGTTCTTGCGCTTTGAAGGTGTGGGGACTTGTACGGAAGTGTATGTCAACGGTTATCTGGTGGGTACACACAAGGGAGGATATT 508 0 218 .......................................................................................... 307 2 381 .......................................................................................... 470 S A F V C E I G E Q V K Y G E S N E I V V K A D N T A R P D Query_1 509 CCGCCTTTGTCTGTGAGATTGGTGAGCAGGTAAAATATGGGGAGAGCAATGAGATTGTAGTGAAGGCTGACAATACAGCTCGTCCGGATG 598 0 308 .......................................................................................... 397 2 471 .......................................................................................... 560 V I P V N H S L F G V Y G G I Y R P V S L V V T E P C H I A Query_1 599 TCATTCCGGTGAACCATAGCCTTTTTGGAGTATACGGAGGTATCTATCGCCCGGTGTCGTTGGTAGTGACAGAGCCTTGTCATATTGCGG 688 0 398 .......................................................................................... 487 0 1305 ............ 1316 1 874 ...C........... 860 2 561 .......................................................................................... 650 V E D Y A S P G V Y I T Q K N V S K S A A E V N V K V K L D Query_1 689 TGGAAGATTATGCTTCTCCAGGGGTCTATATTACCCAGAAAAATGTGTCGAAAAGTGCAGCGGAAGTAAATGTGAAGGTGAAACTGGACA 778 0 488 .......................................................................................... 577 2 651 .......................................................................................... 740 N A S L Q P E T L E L E N A I Y D A E G K L V K K H V R S Query_1 779 ACGC-TTCCTTGCAACCGGAAACACTGGAACTGGAGAATGCAATCTATGATGCGGAAGGCAAACTGGTGAAGAAGCATGTACGCAG-TTT 866 0 578 ....-.................................................................................-... 665 2 741 ....T..................................................................N..............T... 830 L E L T P Q G M Q S Y T A S F Q L K R P H L W Q G R K D P Query_1 867 GGAACTGACTCCGCAAGGAA-TGCAGTCG-TATACGGCTT--CTTTCCAATTAAAACGTCCGCATTTGTGGCAGGGACGGAAAGATCCTT 952 0 666 ....................-........-..........--................................................ 751 2 831 .N..................N........T..........TCT...............N............................... 920 Y L Y K V V S R L K Q E G R V I D E V V Q P L G I R K Y E Query_1 953 --ATTTATATAAGGTAGTATCCCGTCTGAAGCAGGAGGGACGGGTGATTGATGAAGTGGTACAGCCGTTGGGT-ATCCGTAAATATGAAA 1039 0 752 --.......................................................................-................ 838 0 1852 ...-......... 1863 1 319 ...-......... 308 2 921 TAT..............................T.......-.....-...........ACN..N......N.A................ 1008 I V A G K G F F L N G E K Y P M Y G V T R H Q D W W G Query_1 1040 TTGTGGCCGGGAAAGGCTTCTTCCTGAATGG-AGAGAAATA-TCCTAT-GTATGGAGTAACT-CGCCA-TCAGGACTG---GTGGGGAAA 1121 0 839 ...............................-.........-......-.............-.....-.........---......... 920 2 1009 ...N......C..................N.A.N.N.....N......G..N....N.....C..T..T.......NNGGT....N.... 1098 K G S A L T N Q E H D A D L A M I M D I G A T T I R L A H Y Query_1 1122 AGGTAGTGCGTTGACAAATCAGGAACATGACGCGGATTTAGCCATGATTATGGATATTGGAGCTACTACCATTCGCCTGGCACATTATCA 1211 0 921 .......................................................................................... 1010 2 1099 . 1099 Q Q A E Y M Y A K C D S L G L I V W A E I P F V N R V T G Q Query_1 1212 GCAGGCAGAGTATATGTATGCTAAATGCGATAGTCTGGGACTGATAGTCTGGGCTGAGATTCCTTTTGTAAATCGGGTGACGGGGCAGGA 1301 0 1011 .......................................................................................... 1100 1 1091 ...N..... 1083 E W D N A H Q Q M R E L V R Q N F N H P S I Y I W G I H Query_1 1302 A-TGGGATAA--TGCCCATCAGCAG-ATGAGAG-AATTGGTT-CGACAGAATTTTAATCACCCTTCCATTTATATTTGGGGAATTCATAA 1385 0 1101 .-........--.............-.......-........-............................................... 1184 1 1082 .A...N....ATNC.........N.N....NN.N........T...........N.-..........-............-.......N. 996 N Q V Y H P H G Y T A A L T R S L H D V C K T D D P D R Y T Query_1 1386 TCAGGTGTATCATCCGCATGGTTACAC-AGCTGCATTGACCCGTTCGTTGCATGATGTATGTAAGACGGATGATCCGGACCGTTAT-ACG 1473 0 1185 .G.........................-..........................................................-... 1272 1 995 ...........................C.......N................N......N......NC..N...............N... 906 2 858 ......-... 866 A A V N G Y G H A E H P V N Q N A D V Q G M N R Y F G W Query_1 1474 GCTGCTGTAAACGGGTATGGACA-TGCTGAACATCC-GGTGAACCA-GAATGCAGACGTTCAAGGGATGAACCGCTACTTT-GGCTGGTA 1559 0 1273 .......................-............-.........-..................................-........ 1358 0 402 ...-......... 413 1 905 ...N...................A......G.....C.........A..................................T........ 816 2 565 ...-......... 576 2 867 ... 869 Y E R K I Q D I K P W V E G L E K N Y P W Q K L M L T E Y Query_1 1560 TGAAAGGAAGATACAGGATATCAAACCTTGGGTGGAA-GGTTTGGaaaaaaaTT-ACCCCTGG-CAGAAACTGATGTTGACAGAGTATGG 1646 0 1359 .....................................-................-........-.......................... 1445 1 815 .....................................A................N........G.......................... 726 G A D A N L E H Q T E Y L G D A L N W G K P F Y P E T F Q T Query_1 1647 GGCGGATGCGAACTTGGAGCATCAGACTGAATATCTGGGAGATGCCTTGAATTGGGGAAAACCTTTTTATCCTGAGACTTTCCAGACAAA 1736 0 1446 .......................................................................................... 1535 1 725 .......................................................................................... 636 K V H E Y Q W S V I A E H P Y I I A S Y L W N M F D F A V P Query_1 1737 AGTACATGAGTATCAGTGGAGCGTGATTGCTGAACACCCTTACATTATTGCTTCTTACTTGTGGAATATGTTTGATTTTGCTGTGCCTAT 1826 0 1536 .......................................................................................... 1625 1 635 .......................................................................................... 546 M W E R G G V P A R N L K G M V T F D R K I K K D S Y Y W Y Query_1 1827 GTGGGAGCGTGGAGGAGTTCCTGCACGTAACTTGAAGGGAATGGTAACTTTCGACCGTAAGATTAAGAAGGATTCCTATTATTGGTATAA 1916 0 1626 .......................................................................................... 1715 1 545 .......................................................................................... 456 K A N W S K E P V L Y L T Q R R N V D R E R K E T S V T V Y Query_1 1917 AGCTAACTGGAGTAAGGAACCTGTACTTTATCTGACACAGCGTCGTAATGTGGACAGAGAAAGAAAAGAAACTTCGGTAACCGTGTATTC 2006 0 1716 .......................................................................................... 1805 1 455 .......................................................................................... 366 2 139 ............ 150 S N I G T P V V Y L N G K P L E G I R K G Y T E V H Y V F D Query_1 2007 TAATATCGGAACTCCGGTGGTATATCTGAACGGGAAACCTTTGGAAGGTATCCGTAAAGGATATACAGAGGTGCACTATGTATTTGATAA 2096 0 1806 .......................................................................................... 1895 0 820 ............ 831 1 365 .......................................................................................... 276 K V T L V Q G K N I L K A V V S Y Q G K E Y V D E I E W N Y Query_1 2097 AGTGACGCTGGTTCAGGGAAAGAATATATTGAAGGCTGTAGTCAGCTATCAGGGAAAAGAGTACGTAGATGAGATAGAATGGAACTATAC 2186 0 1896 .......................................................................................... 1985 1 275 .......................................................................................... 186 T G E K N R G T V V H E N K K E H A G W * H W K W I T D P N Query_1 2187 TGGTGAAAAGAATAGGGGGACTGTCGTGCATGAGAATAAGAAGGAGCATGCAGGATGGTAACATTGGAAGTGGATAACGGATCCGAATTC 2276 0 1986 .......................................................... 2043 1 185 .......................................................................................... 96 S S S V D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 2277 GAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAG-CCCGAAAGGAAG 2362 1 95 ..................N...............---.....................................C........A... 12 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 10336612 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,303 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200128.107 (CMR200128.23-703.P(703).E502Q.pMCSG68)
REVISFNDGWLFKKGPFATDVMKAAAQWESKWTEVTIPHTWNAQDMQVKTNSFYEGAAYYRKTYVIPETMKGKRLFLRFEGVGTCTEVYVNGYLVGTHKGGYSAFVCEIGEQVKYGESNEIVVKADNTARPDVIPVNHSLFGVYGGIYRPVSLVVTEPCHIAVEDYASPGVYITQKNVSKSAAEVNVKVKLDNASLQPETLELENAIYDAEGKLVKKHVRSLELTPQGMQSYTASFQLKRPHLWQGRKDPYLYKVVSRLKQEGRVIDEVVQPLGIRKYEIVAGKGFFLNGEKYPMYGVTRHQDWWGKGSALTNQEHDADLAMIMDIGATTIRLAHYQQAEYMYAKCDSLGLIVWAEIPFVNRVTGQEWDNAHQQMRELVRQNFNHPSIYIWGIHNEVYHPHGYTAALTRSLHDVCKTDDPDRYTAAVNGYGHAEHPVNQNADVQGMNRYFGWYERKIQDIKPWVEGLEKNYPWQKLMLTEYGADANLEHQTEYLGDALNWGKPFYPETFQTKVHEYQWSVIAEHPYIIASYLWNMFDFAVPMWERGGVPARNLKGMVTFDRKIKKDSYYWYKANWSKEPVLYLTQRRNVDRERKETSVTVYSNIGTPVVYLNGKPLEGIRKGYTEVHYVFDKVTLVQGKNILKAVVSYQGKEYVDEIEWNYTGEKNRGTVVHENKKEHAGW
AGAGAGGTAATCAGCTTTAATGATGGCTGGTTGTTTAAAAAAGGGCCGTTTGCAACAGACGTGATGAAGGCAGCTGCCCAGTGGGAGAGTAAATGGACAGAGGTGACCATCCCTCATACCTGGAATGCGCAGGATATGCAGGTGAAGACAAACTCTTTTTATGAGGGAGCGGCTTATTATCGTAAGACTTATGTGATTCCCGAGACCATGAAGGGGAAGCGTTTGTTCTTGCGCTTTGAAGGTGTGGGGACTTGTACGGAAGTGTATGTCAACGGTTATCTGGTGGGTACACACAAGGGAGGATATTCCGCCTTTGTCTGTGAGATTGGTGAGCAGGTAAAATATGGGGAGAGCAATGAGATTGTAGTGAAGGCTGACAATACAGCTCGTCCGGATGTCATTCCGGTGAACCATAGCCTTTTTGGAGTATACGGAGGTATCTATCGCCCGGTGTCGTTGGTAGTGACAGAGCCTTGTCATATTGCGGTGGAAGATTATGCTTCTCCAGGGGTCTATATTACCCAGAAAAATGTGTCGAAAAGTGCAGCGGAAGTAAATGTGAAGGTGAAACTGGACAACGCTTCCTTGCAACCGGAAACACTGGAACTGGAGAATGCAATCTATGATGCGGAAGGCAAACTGGTGAAGAAGCATGTACGCAGTTTGGAACTGACTCCGCAAGGAATGCAGTCGTATACGGCTTCTTTCCAATTAAAACGTCCGCATTTGTGGCAGGGACGGAAAGATCCTTATTTATATAAGGTAGTATCCCGTCTGAAGCAGGAGGGACGGGTGATTGATGAAGTGGTACAGCCGTTGGGTATCCGTAAATATGAAATTGTGGCCGGGAAAGGCTTCTTCCTGAATGGAGAGAAATATCCTATGTATGGAGTAACTCGCCATCAGGACTGGTGGGGAAAAGGTAGTGCGTTGACAAATCAGGAACATGACGCGGATTTAGCCATGATTATGGATATTGGAGCTACTACCATTCGCCTGGCACATTATCAGCAGGCAGAGTATATGTATGCTAAATGCGATAGTCTGGGACTGATAGTCTGGGCTGAGATTCCTTTTGTAAATCGGGTGACGGGGCAGGAATGGGATAATGCCCATCAGCAGATGAGAGAATTGGTTCGACAGAATTTTAATCACCCTTCCATTTATATTTGGGGAATTCATAATGAGGTGTATCATCCGCATGGTTACACAGCTGCATTGACCCGTTCGTTGCATGATGTATGTAAGACGGATGATCCGGACCGTTATACGGCTGCTGTAAACGGGTATGGACATGCTGAACATCCGGTGAACCAGAATGCAGACGTTCAAGGGATGAACCGCTACTTTGGCTGGTATGAAAGGAAGATACAGGATATCAAACCTTGGGTGGAAGGTTTGGAAAAAAATTACCCCTGGCAGAAACTGATGTTGACAGAGTATGGGGCGGATGCGAACTTGGAGCATCAGACTGAATATCTGGGAGATGCCTTGAATTGGGGAAAACCTTTTTATCCTGAGACTTTCCAGACAAAAGTACATGAGTATCAGTGGAGCGTGATTGCTGAACACCCTTACATTATTGCTTCTTACTTGTGGAATATGTTTGATTTTGCTGTGCCTATGTGGGAGCGTGGAGGAGTTCCTGCACGTAACTTGAAGGGAATGGTAACTTTCGACCGTAAGATTAAGAAGGATTCCTATTATTGGTATAAAGCTAACTGGAGTAAGGAACCTGTACTTTATCTGACACAGCGTCGTAATGTGGACAGAGAAAGAAAAGAAACTTCGGTAACCGTGTATTCTAATATCGGAACTCCGGTGGTATATCTGAACGGGAAACCTTTGGAAGGTATCCGTAAAGGATATACAGAGGTGCACTATGTATTTGATAAAGTGACGCTGGTTCAGGGAAAGAATATATTGAAGGCTGTAGTCAGCTATCAGGGAAAAGAGTACGTAGATGAGATAGAATGGAACTATACTGGTGAAAAGAATAGGGGGACTGTCGTGCATGAGAATAAGAAGGAGCATGCAGGATGG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,395 total letters Query= CMR200128.107 Length=2448 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3680 0.0 2_08F_F8_CMR200128.107_T7S.seq 1793 0.0 1_05F_F8_CMR200128.107_T7term.seq 1790 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 56 AATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGT 145 2 15 .......................................................................................... 104 W S H P Q F E K G T E N L Y F Q S N A R E V I S F N D G W L Query_1 146 GGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCAGAGAGGTAATCAGCTTTAATGATGGCTGGTTGT 235 0 1 .................................. 34 0 1732 ............ 1743 2 105 .......................................................................................... 194 1 442 ............ 431 F K K G P F A T D V M K A A A Q W E S K W T E V T I P H T W Query_1 236 TTAAAAAAGGGCCGTTTGCAACAGACGTGATGAAGGCAGCTGCCCAGTGGGAGAGTAAATGGACAGAGGTGACCATCCCTCATACCTGGA 325 0 35 .......................................................................................... 124 2 195 .......................................................................................... 284 N A Q D M Q V K T N S F Y E G A A Y Y R K T Y V I P E T M K Query_1 326 ATGCGCAGGATATGCAGGTGAAGACAAACTCTTTTTATGAGGGAGCGGCTTATTATCGTAAGACTTATGTGATTCCCGAGACCATGAAGG 415 0 125 .......................................................................................... 214 2 285 .......................................................................................... 374 G K R L F L R F E G V G T C T E V Y V N G Y L V G T H K G G Query_1 416 GGAAGCGTTTGTTCTTGCGCTTTGAAGGTGTGGGGACTTGTACGGAAGTGTATGTCAACGGTTATCTGGTGGGTACACACAAGGGAGGAT 505 0 215 .......................................................................................... 304 2 375 .......................................................................................... 464 Y S A F V C E I G E Q V K Y G E S N E I V V K A D N T A R P Query_1 506 ATTCCGCCTTTGTCTGTGAGATTGGTGAGCAGGTAAAATATGGGGAGAGCAATGAGATTGTAGTGAAGGCTGACAATACAGCTCGTCCGG 595 0 305 .......................................................................................... 394 2 465 .......................................................................................... 554 D V I P V N H S L F G V Y G G I Y R P V S L V V T E P C H I Query_1 596 ATGTCATTCCGGTGAACCATAGCCTTTTTGGAGTATACGGAGGTATCTATCGCCCGGTGTCGTTGGTAGTGACAGAGCCTTGTCATATTG 685 0 395 .......................................................................................... 484 0 1305 ............ 1316 2 555 .......................................................................................... 644 A V E D Y A S P G V Y I T Q K N V S K S A A E V N V K V K L Query_1 686 CGGTGGAAGATTATGCTTCTCCAGGGGTCTATATTACCCAGAAAAATGTGTCGAAAAGTGCAGCGGAAGTAAATGTGAAGGTGAAACTGG 775 0 485 .......................................................................................... 574 2 645 .......................................................................................... 734 D N A S L Q P E T L E L E N A I Y D A E G K L V K K H V R S Query_1 776 ACAACGCTTCCTTGCAACCGGAAACACTGGAACTGGAGAATGCAATCTATGATGCGGAAGGCAAACTGGTGAAGAAGCATGTACGCAGTT 865 0 575 .......................................................................................... 664 2 735 .......................................................................................... 824 L E L T P Q G M Q S Y T A S F Q L K R P H L W Q G R K D P Query_1 866 TGGAACTGACTCCGCAAGGAATGCAGTCGTATACGGC-TTCTTTCCAATTAAAACGTCCGCA-TTTGTGGCAGGGACGGAAAGATCC-TT 952 0 665 .....................................-........................-........................-.. 751 2 825 ...............-.....................T............T.....-N....T................-.......T.. 911 Y L Y K V V S R L K Q E G R V I D E V V Q P L G I R K Y E I Query_1 953 ATTTATATAAGGTAGTATCCCGTCTGAAGCAGGAGGGACGGGTGATTGATGAAGTGGTACAGCCGTTGGGTATCCGTAAATATGAAATTG 1042 0 752 .......................................................................................... 841 0 1852 ............ 1863 2 912 ......N...-..........N...............................N.......-N..........-...-...N........ 997 1 322 ............ 311 V A G K G F F L N G E K Y P M Y G V T R H Q D W W G K G S Query_1 1043 TGGCCGGGAAAGGC-TTCTTCCTGAATGGAGAGAAA-TATCCTATGTATGGAGTAACTCGCCATCAGGACT-GGTGGGGAAAAGGTAGTG 1129 0 842 ..............-.....................-..................................-.................. 928 2 998 GN......N.....T.....N......-........T.NNT..N.NG.......N.......N........G..C.C............. 1086 A L T N Q E H D A D L A M I M D I G A T T I R L A H Y Q Q Query_1 1130 CGTTGACAAATCAGGAACA-TGACGCGGATTTAGCCATGA-TTATGGATATTGGAGC-TACTACCATTCGCCTGGCACATTATCAGCAGG 1216 0 929 ...................-....................-................-................................ 1015 2 1087 ......NNNT..-T.....N.........-..N...N...T..N...N.......A.T.....N.CA...N.... 1159 A E Y M Y A K C D S L G L I V W A E I P F V N R V T G Q E Query_1 1217 CAGAGTATATGTATGCTAAATGCGATAGTCTGGGACTGATAGTCTGGGCTGAGATTCCTTTTGTAAATCGGGTGACGGGGCA--GGAATG 1304 0 1016 ..................................................................................--...... 1103 1 1103 ..N.........NAG...... 1083 W D N A H Q Q M R E L V R Q N F N H P S I Y I W G I H N Query_1 1305 GGATAA-TGCCCATC-AGCAGATGA-GAG-AATTGGTT-CGACAGAATTTTAATCACCCTTCCATTTATATTTGGGG-AATTCATAA-TG 1387 0 1104 ......-........-.........-...-........-......................................-.........-.. 1186 1 1082 ......A.NN.....C.........A..NN........T...........................A..........G.........A.. 993 E V Y H P H G Y T A A L T R S L H D V C K T D D P D R Y T Query_1 1388 A-GGTGTATCATCCGCATGGTTACACAGCTGCATTGA-CCCGTTCGTTGCA-TGATGTATGTAAGACGGATGATCCGGACCGTTATACGG 1474 0 1187 .-...................................-.............-...................................... 1273 1 992 NN....NN.............................N.............N..................................T... 903 A A V N G Y G H A E H P V N Q N A D V Q G M N R Y F G W Y E Query_1 1475 CTGCTGTAAACGGGTATGGACATGCTGAACATCC-GGTGAACCAGAATGCAGACGTTCAAGGGATGAACCGCTACTTTGGCTGGTATGAA 1563 0 1274 ..................................-....................................................... 1362 0 402 ...-......... 413 2 562 ...-......... 573 1 902 ............................G.....N....................................................... 813 R K I Q D I K P W V E G L E K N Y P W Q K L M L T Q Y G A D Query_1 1564 AGGAAGATACAGGATATCAAACCTTGGGTGGAAGGTTTGGaaaaaaaTTACCCCTGGCAGAAACTGATGTTGACACAGTATGGGGCGGAT 1653 0 1363 ...........................................................................G.............. 1452 1 812 .....................................N.................................................... 723 A N L E H Q T E Y L G D A L N W G K P F Y P E T F Q T K V Query_1 1654 GCG-AACTTGGAGCATCAGACTGAATATCTGGGAGATGCCTTGAATTGGGGAAAACCTTTTTATCCTGAGACTTTCCAGACAAAAGTACA 1742 0 1453 ...-...................................................................................... 1541 1 722 ...R...................................................................................... 633 H E Y Q W S V I A E H P Y I I A S Y L W N M F D F A V P M W Query_1 1743 TGAGTATCAGTGGAGCGTGATTGCTGAACACCCTTACATTATTGCTTCTTACTTGTGGAATATGTTTGATTTTGCTGTGCCTATGTGGGA 1832 0 1542 .......................................................................................... 1631 1 632 .......................................................................................... 543 E R G G V P A R N L K G M V T F D R K I K K D S Y Y W Y K A Query_1 1833 GCGTGGAGGAGTTCCTGCACGTAACTTGAAGGGAATGGTAACTTTCGACCGTAAGATTAAGAAGGATTCCTATTATTGGTATAAAGCTAA 1922 0 1632 .......................................................................................... 1721 1 542 .......................................................................................... 453 N W S K E P V L Y L T Q R R N V D R E R K E T S V T V Y S N Query_1 1923 CTGGAGTAAGGAACCTGTACTTTATCTGACACAGCGTCGTAATGTGGACAGAGAAAGAAAAGAAACTTCGGTAACCGTGTATTCTAATAT 2012 0 1722 .......................................................................................... 1811 2 136 ............ 147 1 452 .......................................................................................... 363 I G T P V V Y L N G K P L E G I R K G Y T E V H Y V F D K V Query_1 2013 CGGAACTCCGGTGGTATATCTGAACGGGAAACCTTTGGAAGGTATCCGTAAAGGATATACAGAGGTGCACTATGTATTTGATAAAGTGAC 2102 0 1812 .......................................................................................... 1901 0 820 ............ 831 1 362 .......................................................................................... 273 T L V Q G K N I L K A V V S Y Q G K E Y V D E I E W N Y T G Query_1 2103 GCTGGTTCAGGGAAAGAATATATTGAAGGCTGTAGTCAGCTATCAGGGAAAAGAGTACGTAGATGAGATAGAATGGAACTATACTGGTGA 2192 0 1902 .......................................................................................... 1991 1 272 .......................................................................................... 183 E K N R G T V V H E N K K E H A G W * H W K W I T D P N S S Query_1 2193 AAAGAATAGGGGGACTGTCGTGCATGAGAATAAGAAGGAGCATGCAGGATGGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTC 2282 0 1992 .................................................... 2043 1 182 .......................................................................................... 93 S V D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 2283 CGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCG--AAAGGAAG 2362 1 92 ............................---.........................................AA........ 14 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 10560264 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,395 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200133.104 (CMR200133.20-1037.P(1037).E465A.pMCSG68)
QTFKEWQDPEINAINRAPMHANFFAYENAEMAAKAVKEDSKNFMSLNGTWKFFWVRNADARPTDFWKTGFNDKGWDNLQVPGVWETHGYGDPIYINVGYGWRSRFENNPPYVPTEENHVGSYRREIMVPADWKGKDIIAHFGAVSSNMYLWVNGKFVGYSEDSKLEAEFNLTPYLKPGQKNLIAFQVFRWCDGSYLEDQDFFRYTGVARDCYLYTRNKKRIDDIRVTPDLDSEYKNGSLAINLNLKGSGNVSLELLDAQNQTVASTEVKGSGNVSTTLQVENPKKWSAETPYLYTLRATLKDGSQASEVVPVKVGFRKIELKNAQILVNGQPVLFKGADRHELDPDGGYVVSRDRMIQDIQIMKQFNINAVRTCHYPDDNFWYELCDKYGIYVVAEANIESHGMGYGDKTLAKEPTYKKAHMERNQRNVQRGFNHPSIIFWSLGNEAGYGPNFEAAYDWIKAEDPSRAVQYEQARIEGKTDIYCPMYANYKWCEEYSKDDKYQKPLIQCEYAHAMGNSQGGFKEYWDLIRKYPKYQGGFIWDFVDQSIRWTGKNGKMIYAYAGDFNRMDDSGDKNFCNNGLISPDRKPNPHAYEVQYFYQNIWTTPGDLAKGEIKVYNENFFRDLSAYALEWEVLKDGKAIRSGRIENLNVAPHQTASLKLDLGKTCQCGEWLLNVKYIQKNREGLIPAGFVVAKDQLVLNPYKAPAMELKNVVETNQQVVEPQINPNYPDYITVEGDGFYLEFSKRNGYLDRYDVNGVSMLKEGEALTPNFWRAPTDNDFGAGLQNKYAAWKNPGIKLTDFNYKVENGQAIVNSTYDLKEVSAKLYLTYQINNQGAVKVTQKMVADKNAKVSPMFRFGMQMVMPKSFEKISYYGRGPIENYSDRNHCTDLGIYNQNVSDQFYPYIRPQENGNKTDIRWWKQVNMAGNGLMFVAEAPFSASALHYTIASLDDGTEKKQSHSFEVEEADLTNLLIDKAQMGLGCVNSWGALPEDKYMLPYGDYEFTFIMTPVKGYIAHE
CAGACATTTAAAGAATGGCAAGACCCGGAAATAAATGCCATCAACCGGGCACCGATGCACGCAAACTTCTTTGCGTACGAAAATGCAGAAATGGCTGCCAAAGCAGTCAAGGAAGACTCCAAGAACTTTATGAGCCTGAATGGAACCTGGAAATTCTTCTGGGTGCGGAATGCAGATGCACGCCCCACGGATTTCTGGAAAACCGGCTTCAACGATAAAGGTTGGGACAACCTTCAGGTGCCGGGAGTATGGGAAACTCACGGATACGGCGACCCTATCTACATCAACGTAGGCTACGGATGGCGCAGCCGTTTCGAGAACAATCCTCCTTACGTACCGACTGAAGAAAACCACGTAGGTTCTTACCGTCGTGAAATTATGGTTCCGGCCGACTGGAAAGGCAAAGACATCATCGCTCATTTTGGTGCAGTCAGCTCAAACATGTACCTGTGGGTAAACGGTAAGTTCGTTGGCTACAGCGAAGACAGCAAGCTGGAAGCTGAATTCAACCTGACTCCGTACCTGAAACCGGGACAGAAAAACCTGATTGCTTTCCAGGTGTTCCGCTGGTGTGACGGTTCATACCTGGAAGACCAGGACTTCTTCCGCTATACCGGAGTAGCACGCGACTGCTATCTTTATACCCGCAACAAAAAACGTATCGATGACATCCGCGTGACTCCCGACCTGGACAGCGAATACAAGAACGGTTCACTGGCCATCAACCTGAACCTGAAAGGAAGCGGCAATGTCAGCCTGGAACTGCTGGATGCCCAAAACCAGACCGTAGCTTCTACCGAAGTAAAAGGTTCAGGCAATGTTTCTACTACACTTCAGGTAGAAAATCCGAAAAAATGGAGCGCAGAAACTCCTTACTTATACACACTGCGTGCTACACTGAAAGACGGTAGCCAGGCTAGCGAAGTTGTACCTGTGAAAGTAGGTTTCCGCAAGATTGAACTCAAAAACGCACAGATTCTGGTCAACGGCCAGCCTGTACTTTTCAAAGGTGCCGACCGTCATGAACTGGACCCGGACGGTGGATACGTAGTTTCACGCGACCGCATGATTCAGGACATTCAGATTATGAAGCAGTTCAACATCAACGCCGTAAGAACCTGTCACTATCCGGACGACAACTTCTGGTACGAACTCTGCGACAAATACGGTATTTATGTAGTGGCTGAAGCCAACATCGAATCTCACGGAATGGGATACGGCGACAAGACACTGGCCAAGGAACCGACTTATAAAAAGGCACACATGGAACGCAACCAGCGCAACGTACAGCGTGGTTTCAACCATCCGAGCATCATCTTCTGGTCATTGGGTAACGAGGCCGGATACGGTCCGAACTTCGAAGCTGCTTACGACTGGATCAAGGCAGAAGACCCCAGCCGTGCCGTACAATACGAACAGGCCCGCATCGAAGGAAAGACTGATATCTACTGCCCGATGTATGCCAACTACAAATGGTGCGAAGAATACAGCAAGGACGATAAATACCAGAAACCGCTGATTCAGTGTGAATATGCACACGCCATGGGTAATTCACAAGGTGGATTCAAGGAATACTGGGACCTGATTCGCAAGTATCCGAAATATCAGGGTGGATTCATCTGGGACTTCGTAGACCAGTCTATCCGCTGGACAGGAAAGAACGGCAAGATGATTTATGCTTATGCCGGCGACTTCAACCGTATGGATGATTCTGGCGACAAGAACTTCTGTAACAACGGACTTATCAGCCCTGACCGTAAACCGAATCCGCATGCATACGAAGTACAGTACTTCTACCAGAACATCTGGACTACTCCGGGCGATTTGGCAAAGGGAGAAATCAAGGTATACAACGAAAACTTCTTCCGTGACCTGTCTGCATACGCACTGGAATGGGAAGTACTGAAAGATGGTAAGGCTATCCGTAGCGGACGCATTGAAAACCTGAACGTGGCACCTCATCAGACTGCCAGCCTGAAACTTGATCTGGGCAAGACTTGCCAGTGTGGTGAATGGCTGCTCAACGTGAAATACATCCAGAAGAACCGTGAAGGTTTGATCCCGGCCGGTTTTGTAGTAGCTAAAGACCAGCTTGTGCTGAATCCATACAAAGCACCGGCTATGGAACTGAAGAACGTAGTTGAAACTAACCAGCAGGTAGTTGAACCGCAAATCAACCCGAACTATCCGGACTACATCACTGTAGAAGGCGACGGATTCTATCTGGAATTCAGCAAACGCAACGGTTATCTTGACAGATACGATGTAAACGGAGTAAGCATGTTGAAAGAAGGCGAAGCACTGACACCGAACTTCTGGCGTGCCCCGACCGACAACGACTTCGGTGCTGGTCTGCAGAACAAATATGCTGCATGGAAGAACCCGGGCATCAAACTGACAGACTTCAACTACAAGGTAGAAAACGGACAGGCCATCGTAAACTCTACTTACGACCTCAAGGAAGTATCTGCCAAGTTGTATCTCACTTACCAGATCAACAACCAGGGAGCAGTGAAAGTGACACAGAAAATGGTAGCCGACAAGAATGCCAAGGTATCACCGATGTTCCGTTTCGGTATGCAGATGGTAATGCCGAAGTCATTTGAAAAGATTTCTTACTACGGACGCGGTCCGATAGAAAACTACAGCGACCGTAACCATTGCACTGACCTGGGAATCTACAACCAGAATGTAAGCGACCAGTTCTATCCGTACATCCGTCCGCAGGAAAACGGCAACAAGACTGACATCCGCTGGTGGAAACAGGTAAACATGGCTGGAAACGGACTGATGTTCGTAGCTGAAGCACCGTTCTCTGCTTCTGCTCTCCACTACACCATCGCTTCACTGGACGACGGTACTGAAAAGAAACAGAGCCACTCGTTCGAAGTAGAAGAAGCCGACCTGACCAACCTGTTGATTGACAAGGCACAGATGGGCTTAGGTTGTGTCAACAGCTGGGGTGCCCTGCCGGAAGACAAGTACATGCTTCCTTACGGAGATTATGAATTTACGTTCATCATGACTCCGGTGAAAGGCTATATCGCACACGAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,561 total letters Query= CMR200133.104 Length=3459 Score E Sequences producing significant alignments: (Bits) Value 0_wt 5499 0.0 1_05G_G8_CMR200133.104_T7term.seq 1795 0.0 2_08G_G8_CMR200133.104_T7S.seq 91.5 6e-21 * Q F P S R N N F V * L * E G D I H M H H H H H H S S G V D Query_1 52 TAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGAT 141 2 12 ....TT.C.................................................A.. 71 L W S H P Q F E K G T E N L Y F Q S N A Q T F K E W Q D P E Query_1 142 CTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAGACATTTAAAGAATGGCAAGACCCGGAA 231 0 1 .............................. 30 I N A I N R A P M H A N F F A Y E N A E M A A K A V K E D S Query_1 232 ATAAATGCCATCAACCGGGCACCGATGCACGCAAACTTCTTTGCGTACGAAAATGCAGAAATGGCTGCCAAAGCAGTCAAGGAAGACTCC 321 0 31 .......................................................................................... 120 K N F M S L N G T W K F F W V R N A D A R P T D F W K T G F Query_1 322 AAGAACTTTATGAGCCTGAATGGAACCTGGAAATTCTTCTGGGTGCGGAATGCAGATGCACGCCCCACGGATTTCTGGAAAACCGGCTTC 411 0 121 .......................................................................................... 210 N D K G W D N L Q V P G V W E T H G Y G D P I Y I N V G Y G Query_1 412 AACGATAAAGGTTGGGACAACCTTCAGGTGCCGGGAGTATGGGAAACTCACGGATACGGCGACCCTATCTACATCAACGTAGGCTACGGA 501 0 211 .......................................................................................... 300 0 1213 ............ 1224 W R S R F E N N P P Y V P T E E N H V G S Y R R E I M V P A Query_1 502 TGGCGCAGCCGTTTCGAGAACAATCCTCCTTACGTACCGACTGAAGAAAACCACGTAGGTTCTTACCGTCGTGAAATTATGGTTCCGGCC 591 0 301 .......................................................................................... 390 D W K G K D I I A H F G A V S S N M Y L W V N G K F V G Y S Query_1 592 GACTGGAAAGGCAAAGACATCATCGCTCATTTTGGTGCAGTCAGCTCAAACATGTACCTGTGGGTAAACGGTAAGTTCGTTGGCTACAGC 681 0 391 .......................................................................................... 480 E D S K L E A E F N L T P Y L K P G Q K N L I A F Q V F R W Query_1 682 GAAGACAGCAAGCTGGAAGCTGAATTCAACCTGACTCCGTACCTGAAACCGGGACAGAAAAACCTGATTGCTTTCCAGGTGTTCCGCTGG 771 0 481 .......................................................................................... 570 C D G S Y L E D Q D F F R Y T G V A R D C Y L Y T R N K K R Query_1 772 TGTGACGGTTCATACCTGGAAGACCAGGACTTCTTCCGCTATACCGGAGTAGCACGCGACTGCTATCTTTATACCCGCAACAAAAAACGT 861 0 571 .......................................................................................... 660 2 785 ....G.T...... 797 I D D I R V T P D L D S E Y K N G S L A I N L N L K G S G N Query_1 862 ATCGATGACATCCGCGTGACTCCCGACCTGGACAGCGAATACAAGAACGGTTCACTGGCCATCAACCTGAACCTGAAAGGAAGCGGCAAT 951 0 661 .......................................................................................... 750 2 798 ...... 803 V S L E L L D A Q N Q T V A S T E V K G S G N V S T T L Q V Query_1 952 GTCAGCCTGGAACTGCTGGATGCCCAAAACCAGACCGTAGCTTCTACCGAAGTAAAAGGTTCAGGCAATGTTTCTACTACACTTCAGGTA 1041 0 751 .......................................................................................... 840 E N P K K W S A E T P Y L Y T L R A T L K D G S Q A S E V V Query_1 1042 GAAAATCCGAAAAAATGGAGCGCAGAAACTCCTTACTTATACACACTGCGTGCTACACTGAAAGACGGTAGCCAGGCTAGCGAAGTTGTA 1131 0 841 .......................................................................................... 930 P V K V G F R K I E L K N A Q I L V N G Q P V L F K G A D R Query_1 1132 CCTGTGAAAGTAGGTTTCCGCAAGATTGAACTCAAAAACGCACAGATTCTGGTCAACGGCCAGCCTGTACTTTTCAAAGGTGCCGACCGT 1221 0 931 .......................................................................................... 1020 H E L D P D G G Y V V S R D R M I Q D I Q I M K Q F N I N A Query_1 1222 CATGAACTGGACCCGGACGGTGGATACGTAGTTTCACGCGACCGCATGATTCAGGACATTCAGATTATGAAGCAGTTCAACATCAACGCC 1311 0 1021 .......................................................................................... 1110 V R T C H Y P D D N F W Y E L C D K Y G I Y V V A E A N I E Query_1 1312 GTAAGAACCTGTCACTATCCGGACGACAACTTCTGGTACGAACTCTGCGACAAATACGGTATTTATGTAGTGGCTGAAGCCAACATCGAA 1401 0 1111 .......................................................................................... 1200 0 2183 ...........T... 2197 1 937 ...........T... 923 S H G M G Y G D K T L A K E P T Y K K A H M E R N Q R N V Q Query_1 1402 TCTCACGGAATGGGATACGGCGACAAGACACTGGCCAAGGAACCGACTTATAAAAAGGCACACATGGAACGCAACCAGCGCAACGTACAG 1491 0 1201 .......................................................................................... 1290 0 262 ............ 273 R G F N H P S I I F W S L G N A A G Y G P N F E A A Y D W I Query_1 1492 CGTGGTTTCAACCATCCGAGCATCATCTTCTGGTCATTGGGTAACGCTGCCGGATACGGTCCGAACTTCGAAGCTGCTTACGACTGGATC 1581 0 1291 ..............................................AG.......................................... 1380 K A E D P S R A V Q Y E Q A R I E G K T D I Y C P M Y A N Y Query_1 1582 AAGGCAGAAGACCCCAGCCGTGCCGTACAATACGAACAGGCCCGCATCGAAGGAAAGACTGATATCTACTGCCCGATGTATGCCAACTAC 1671 0 1381 .......................................................................................... 1470 K W C E E Y S K D D K Y Q K P L I Q C E Y A H A M G N S Q G Query_1 1672 AAATGGTGCGAAGAATACAGCAAGGACGATAAATACCAGAAACCGCTGATTCAGTGTGAATATGCACACGCCATGGGTAATTCACAAGGT 1761 0 1471 .......................................................................................... 1560 G F K E Y W D L I R K Y P K Y Q G G F I W D F V D Q S I R W Query_1 1762 GGATTCAAGGAATACTGGGACCTGATTCGCAAGTATCCGAAATATCAGGGTGGATTCATCTGGGACTTCGTAGACCAGTCTATCCGCTGG 1851 0 1561 .......................................................................................... 1650 T G K N G K M I Y A Y A G D F N R M D D S G D K N F C N N G Query_1 1852 ACAGGAAAGAACGGCAAGATGATTTATGCTTATGCCGGCGACTTCAACCGTATGGATGATTCTGGCGACAAGAACTTCTGTAACAACGGA 1941 0 1651 .......................................................................................... 1740 L I S P D R K P N P H A Y E V Q Y F Y Q N I W T T P G D L A Query_1 1942 CTTATCAGCCCTGACCGTAAACCGAATCCGCATGCATACGAAGTACAGTACTTCTACCAGAACATCTGGACTACTCCGGGCGATTTGGCA 2031 0 1741 .......................................................................................... 1830 K G E I K V Y N E N F F R D L S A Y A L E W E V L K D G K A Query_1 2032 AAGGGAGAAATCAAGGTATACAACGAAAACTTCTTCCGTGACCTGTCTGCATACGCACTGGAATGGGAAGTACTGAAAGATGGTAAGGCT 2121 0 1831 .......................................................................................... 1920 I R S G R I E N L N V A P H Q T A S L K L D L G K T C Q C Query_1 2122 ATCCGTAGCGGACGCATTGAAAACCTGAACGTGGCACCTCATCAGACTGCCAGCCTGAAACTTGATCTGGGCAAGACTTGC-CAGTGT-- 2208 0 1921 .................................................................................-......-- 2007 1 1147 .........NN...N..TN 1129 2 519 ..G........... 532 G E W L L N V K Y I Q K N R E G L I P A G F V V A K Query_1 2209 -GGTGAATGGCTGC-TCAACG-TGAAA--TACATCCA-GAAGAACCGTGAAGGTTTGAT-CCCGGCCGG--TTTTGTA-GTAGCTAAA-- 2286 0 2008 -.............-......-.....--........-.....................-.........--.......-.........-- 2085 1 1128 G.......N.....N...N..G.....ATNN.N....N..................N..N....N....NT.......A.........AG 1039 D Q L V L N P Y K A P A M E L K N V V E T N Q Q V V E P Query_1 2287 GACCAGCTTGT-GCTGAA--TCCATACAAA-GCACCGGCTATGGAACTGAAGAACGTAGTTGAAACTAACCAGCAGGTAGTTGAACCGCA 2372 0 2086 ...........-......--..........-........................................................... 2171 1 1038 N....N...N.T....G.AT..........A........................................................... 949 Q I N P N Y P D Y I T V E G D G F Y L E F S K R N G Y L D R Query_1 2373 AATCAACCCGAACTATCCGGACTACATCACTGTAGAAGGCGACGGA-TTCTATCTGGAATTCAGCAAACGCAACGGTTATCTTGACAGAT 2461 0 2172 ..............................................-........................................... 2260 0 1124 ...........G... 1138 1 948 .............................................NN....................-...................... 860 Y D V N G V S M L K E G E A L T P N F W R A P T D N D F G A Query_1 2462 ACGATGTAAACGGAGTAAGCATGTTGAAAGAAGGCGAAGCACTGACACCGAACTTCTGGCGTGCCCCGACCGACAACGACTTCGGTGCTG 2551 0 2261 .......................................................................................... 2350 1 859 .......................................................................................... 770 G L Q N K Y A A W K N P G I K L T D F N Y K V E N G Q A I V Query_1 2552 GTCTGCAGAACAAATATGCTGCATGGAAGAACCCGGGCATCAAACTGACAGACTTCAACTACAAGGTAGAAAACGGACAGGCCATCGTAA 2641 0 2351 .......................................................................................... 2440 1 769 .......................................................................................... 680 2 96 ........... 86 N S T Y D L K E V S A K L Y L T Y Q I N N Q G A V K V T Q K Query_1 2642 ACTCTACTTACGACCTCAAGGAAGTATCTGCCAAGTTGTATCTCACTTACCAGATCAACAACCAGGGAGCAGTGAAAGTGACACAGAAAA 2731 0 2441 .......................................................................................... 2530 1 679 .......................................................................................... 590 M V A D K N A K V S P M F R F G M Q M V M P K S F E K I S Y Query_1 2732 TGGTAGCCGACAAGAATGCCAAGGTATCACCGATGTTCCGTTTCGGTATGCAGATGGTAATGCCGAAGTCATTTGAAAAGATTTCTTACT 2821 0 2531 .......................................................................................... 2620 1 589 .......................................................................................... 500 Y G R G P I E N Y S D R N H C T D L G I Y N Q N V S D Q F Y Query_1 2822 ACGGACGCGGTCCGATAGAAAACTACAGCGACCGTAACCATTGCACTGACCTGGGAATCTACAACCAGAATGTAAGCGACCAGTTCTATC 2911 0 2621 .......................................................................................... 2710 1 499 .......................................................................................... 410 P Y I R P Q E N G N K T D I R W W K Q V N M A G N G L M F V Query_1 2912 CGTACATCCGTCCGCAGGAAAACGGCAACAAGACTGACATCCGCTGGTGGAAACAGGTAAACATGGCTGGAAACGGACTGATGTTCGTAG 3001 0 2711 .......................................................................................... 2800 1 409 .......................................................................................... 320 A E A P F S A S A L H Y T I A S L D D G T E K K Q S H S F E Query_1 3002 CTGAAGCACCGTTCTCTGCTTCTGCTCTCCACTACACCATCGCTTCACTGGACGACGGTACTGAAAAGAAACAGAGCCACTCGTTCGAAG 3091 0 2801 .......................................................................................... 2890 1 319 .......................................................................................... 230 V E E A D L T N L L I D K A Q M G L G C V N S W G A L P E D Query_1 3092 TAGAAGAAGCCGACCTGACCAACCTGTTGATTGACAAGGCACAGATGGGCTTAGGTTGTGTCAACAGCTGGGGTGCCCTGCCGGAAGACA 3181 0 2891 .......................................................................................... 2980 1 229 .......................................................................................... 140 K Y M L P Y G D Y E F T F I M T P V K G Y I A H E * H W K W Query_1 3182 AGTACATGCTTCCTTACGGAGATTATGAATTTACGTTCATCATGACTCCGGTGAAAGGCTATATCGCACACGAATAACATTGGAAGTGGA 3271 0 2981 .......................................................................... 3054 1 139 .......................................................................................... 50 I T D P N S S S V D K L A A A L E H H H H H H * D P A A N K Query_1 3272 TAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAG 3361 1 49 ....--..... 41 1 45 .................. 28 A R K E Query_1 3362 CCCGAAAGGAAG 3373 1 27 .......A.... 16 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 18949587 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 5,561 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200133.105 (CMR200133.20-1037.P(1037).E529A.pMCSG68)
QTFKEWQDPEINAINRAPMHANFFAYENAEMAAKAVKEDSKNFMSLNGTWKFFWVRNADARPTDFWKTGFNDKGWDNLQVPGVWETHGYGDPIYINVGYGWRSRFENNPPYVPTEENHVGSYRREIMVPADWKGKDIIAHFGAVSSNMYLWVNGKFVGYSEDSKLEAEFNLTPYLKPGQKNLIAFQVFRWCDGSYLEDQDFFRYTGVARDCYLYTRNKKRIDDIRVTPDLDSEYKNGSLAINLNLKGSGNVSLELLDAQNQTVASTEVKGSGNVSTTLQVENPKKWSAETPYLYTLRATLKDGSQASEVVPVKVGFRKIELKNAQILVNGQPVLFKGADRHELDPDGGYVVSRDRMIQDIQIMKQFNINAVRTCHYPDDNFWYELCDKYGIYVVAEANIESHGMGYGDKTLAKEPTYKKAHMERNQRNVQRGFNHPSIIFWSLGNEAGYGPNFEAAYDWIKAEDPSRAVQYEQARIEGKTDIYCPMYANYKWCEEYSKDDKYQKPLIQCEYAHAMGNSQGGFKEYWDLIRKYPKYQGGFIWDFVDQSIRWTGKNGKMIYAYAGDFNRMDDSGDKNFCNNGLISPDRKPNPHAYEVQYFYQNIWTTPGDLAKGEIKVYNENFFRDLSAYALEWEVLKDGKAIRSGRIENLNVAPHQTASLKLDLGKTCQCGEWLLNVKYIQKNREGLIPAGFVVAKDQLVLNPYKAPAMELKNVVETNQQVVEPQINPNYPDYITVEGDGFYLEFSKRNGYLDRYDVNGVSMLKEGEALTPNFWRAPTDNDFGAGLQNKYAAWKNPGIKLTDFNYKVENGQAIVNSTYDLKEVSAKLYLTYQINNQGAVKVTQKMVADKNAKVSPMFRFGMQMVMPKSFEKISYYGRGPIENYSDRNHCTDLGIYNQNVSDQFYPYIRPQENGNKTDIRWWKQVNMAGNGLMFVAEAPFSASALHYTIASLDDGTEKKQSHSFEVEEADLTNLLIDKAQMGLGCVNSWGALPEDKYMLPYGDYEFTFIMTPVKGYIAHE
CAGACATTTAAAGAATGGCAAGACCCGGAAATAAATGCCATCAACCGGGCACCGATGCACGCAAACTTCTTTGCGTACGAAAATGCAGAAATGGCTGCCAAAGCAGTCAAGGAAGACTCCAAGAACTTTATGAGCCTGAATGGAACCTGGAAATTCTTCTGGGTGCGGAATGCAGATGCACGCCCCACGGATTTCTGGAAAACCGGCTTCAACGATAAAGGTTGGGACAACCTTCAGGTGCCGGGAGTATGGGAAACTCACGGATACGGCGACCCTATCTACATCAACGTAGGCTACGGATGGCGCAGCCGTTTCGAGAACAATCCTCCTTACGTACCGACTGAAGAAAACCACGTAGGTTCTTACCGTCGTGAAATTATGGTTCCGGCCGACTGGAAAGGCAAAGACATCATCGCTCATTTTGGTGCAGTCAGCTCAAACATGTACCTGTGGGTAAACGGTAAGTTCGTTGGCTACAGCGAAGACAGCAAGCTGGAAGCTGAATTCAACCTGACTCCGTACCTGAAACCGGGACAGAAAAACCTGATTGCTTTCCAGGTGTTCCGCTGGTGTGACGGTTCATACCTGGAAGACCAGGACTTCTTCCGCTATACCGGAGTAGCACGCGACTGCTATCTTTATACCCGCAACAAAAAACGTATCGATGACATCCGCGTGACTCCCGACCTGGACAGCGAATACAAGAACGGTTCACTGGCCATCAACCTGAACCTGAAAGGAAGCGGCAATGTCAGCCTGGAACTGCTGGATGCCCAAAACCAGACCGTAGCTTCTACCGAAGTAAAAGGTTCAGGCAATGTTTCTACTACACTTCAGGTAGAAAATCCGAAAAAATGGAGCGCAGAAACTCCTTACTTATACACACTGCGTGCTACACTGAAAGACGGTAGCCAGGCTAGCGAAGTTGTACCTGTGAAAGTAGGTTTCCGCAAGATTGAACTCAAAAACGCACAGATTCTGGTCAACGGCCAGCCTGTACTTTTCAAAGGTGCCGACCGTCATGAACTGGACCCGGACGGTGGATACGTAGTTTCACGCGACCGCATGATTCAGGACATTCAGATTATGAAGCAGTTCAACATCAACGCCGTAAGAACCTGTCACTATCCGGACGACAACTTCTGGTACGAACTCTGCGACAAATACGGTATTTATGTAGTGGCTGAAGCCAACATCGAATCTCACGGAATGGGATACGGCGACAAGACACTGGCCAAGGAACCGACTTATAAAAAGGCACACATGGAACGCAACCAGCGCAACGTACAGCGTGGTTTCAACCATCCGAGCATCATCTTCTGGTCATTGGGTAACGAGGCCGGATACGGTCCGAACTTCGAAGCTGCTTACGACTGGATCAAGGCAGAAGACCCCAGCCGTGCCGTACAATACGAACAGGCCCGCATCGAAGGAAAGACTGATATCTACTGCCCGATGTATGCCAACTACAAATGGTGCGAAGAATACAGCAAGGACGATAAATACCAGAAACCGCTGATTCAGTGTGAATATGCACACGCCATGGGTAATTCACAAGGTGGATTCAAGGAATACTGGGACCTGATTCGCAAGTATCCGAAATATCAGGGTGGATTCATCTGGGACTTCGTAGACCAGTCTATCCGCTGGACAGGAAAGAACGGCAAGATGATTTATGCTTATGCCGGCGACTTCAACCGTATGGATGATTCTGGCGACAAGAACTTCTGTAACAACGGACTTATCAGCCCTGACCGTAAACCGAATCCGCATGCATACGAAGTACAGTACTTCTACCAGAACATCTGGACTACTCCGGGCGATTTGGCAAAGGGAGAAATCAAGGTATACAACGAAAACTTCTTCCGTGACCTGTCTGCATACGCACTGGAATGGGAAGTACTGAAAGATGGTAAGGCTATCCGTAGCGGACGCATTGAAAACCTGAACGTGGCACCTCATCAGACTGCCAGCCTGAAACTTGATCTGGGCAAGACTTGCCAGTGTGGTGAATGGCTGCTCAACGTGAAATACATCCAGAAGAACCGTGAAGGTTTGATCCCGGCCGGTTTTGTAGTAGCTAAAGACCAGCTTGTGCTGAATCCATACAAAGCACCGGCTATGGAACTGAAGAACGTAGTTGAAACTAACCAGCAGGTAGTTGAACCGCAAATCAACCCGAACTATCCGGACTACATCACTGTAGAAGGCGACGGATTCTATCTGGAATTCAGCAAACGCAACGGTTATCTTGACAGATACGATGTAAACGGAGTAAGCATGTTGAAAGAAGGCGAAGCACTGACACCGAACTTCTGGCGTGCCCCGACCGACAACGACTTCGGTGCTGGTCTGCAGAACAAATATGCTGCATGGAAGAACCCGGGCATCAAACTGACAGACTTCAACTACAAGGTAGAAAACGGACAGGCCATCGTAAACTCTACTTACGACCTCAAGGAAGTATCTGCCAAGTTGTATCTCACTTACCAGATCAACAACCAGGGAGCAGTGAAAGTGACACAGAAAATGGTAGCCGACAAGAATGCCAAGGTATCACCGATGTTCCGTTTCGGTATGCAGATGGTAATGCCGAAGTCATTTGAAAAGATTTCTTACTACGGACGCGGTCCGATAGAAAACTACAGCGACCGTAACCATTGCACTGACCTGGGAATCTACAACCAGAATGTAAGCGACCAGTTCTATCCGTACATCCGTCCGCAGGAAAACGGCAACAAGACTGACATCCGCTGGTGGAAACAGGTAAACATGGCTGGAAACGGACTGATGTTCGTAGCTGAAGCACCGTTCTCTGCTTCTGCTCTCCACTACACCATCGCTTCACTGGACGACGGTACTGAAAAGAAACAGAGCCACTCGTTCGAAGTAGAAGAAGCCGACCTGACCAACCTGTTGATTGACAAGGCACAGATGGGCTTAGGTTGTGTCAACAGCTGGGGTGCCCTGCCGGAAGACAAGTACATGCTTCCTTACGGAGATTATGAATTTACGTTCATCATGACTCCGGTGAAAGGCTATATCGCACACGAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,501 total letters Query= CMR200133.105 Length=3459 Score E Sequences producing significant alignments: (Bits) Value 0_wt 5499 0.0 1_05H_H8_CMR200133.105_T7term.seq 1871 0.0 2_08H_H8_CMR200133.105_T7S.seq 69.8 2e-14 S R N N F V * L * E G D I H M H H H H H H S S G V D L W S Query_1 62 CCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTC 151 2 10 .........-............-........................A.. 57 H P Q F E K G T E N L Y F Q S N A Q T F K E W Q D P E I N A Query_1 152 ATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAGACATTTAAAGAATGGCAAGACCCGGAAATAAATGCCA 241 0 1 ........................................ 40 I N R A P M H A N F F A Y E N A E M A A K A V K E D S K N F Query_1 242 TCAACCGGGCACCGATGCACGCAAACTTCTTTGCGTACGAAAATGCAGAAATGGCTGCCAAAGCAGTCAAGGAAGACTCCAAGAACTTTA 331 0 41 .......................................................................................... 130 M S L N G T W K F F W V R N A D A R P T D F W K T G F N D K Query_1 332 TGAGCCTGAATGGAACCTGGAAATTCTTCTGGGTGCGGAATGCAGATGCACGCCCCACGGATTTCTGGAAAACCGGCTTCAACGATAAAG 421 0 131 .......................................................................................... 220 2 988 ............. 976 G W D N L Q V P G V W E T H G Y G D P I Y I N V G Y G W R S Query_1 422 GTTGGGACAACCTTCAGGTGCCGGGAGTATGGGAAACTCACGGATACGGCGACCCTATCTACATCAACGTAGGCTACGGATGGCGCAGCC 511 0 221 .......................................................................................... 310 0 1213 ............ 1224 R F E N N P P Y V P T E E N H V G S Y R R E I M V P A D W K Query_1 512 GTTTCGAGAACAATCCTCCTTACGTACCGACTGAAGAAAACCACGTAGGTTCTTACCGTCGTGAAATTATGGTTCCGGCCGACTGGAAAG 601 0 311 .......................................................................................... 400 G K D I I A H F G A V S S N M Y L W V N G K F V G Y S E D S Query_1 602 GCAAAGACATCATCGCTCATTTTGGTGCAGTCAGCTCAAACATGTACCTGTGGGTAAACGGTAAGTTCGTTGGCTACAGCGAAGACAGCA 691 0 401 .......................................................................................... 490 K L E A E F N L T P Y L K P G Q K N L I A F Q V F R W C D G Query_1 692 AGCTGGAAGCTGAATTCAACCTGACTCCGTACCTGAAACCGGGACAGAAAAACCTGATTGCTTTCCAGGTGTTCCGCTGGTGTGACGGTT 781 0 491 .......................................................................................... 580 S Y L E D Q D F F R Y T G V A R D C Y L Y T R N K K R I D D Query_1 782 CATACCTGGAAGACCAGGACTTCTTCCGCTATACCGGAGTAGCACGCGACTGCTATCTTTATACCCGCAACAAAAAACGTATCGATGACA 871 0 581 .......................................................................................... 670 2 772 ....G.T............ 790 I R V T P D L D S E Y K N G S L A I N L N L K G S G N V S L Query_1 872 TCCGCGTGACTCCCGACCTGGACAGCGAATACAAGAACGGTTCACTGGCCATCAACCTGAACCTGAAAGGAAGCGGCAATGTCAGCCTGG 961 0 671 .......................................................................................... 760 E L L D A Q N Q T V A S T E V K G S G N V S T T L Q V E N P Query_1 962 AACTGCTGGATGCCCAAAACCAGACCGTAGCTTCTACCGAAGTAAAAGGTTCAGGCAATGTTTCTACTACACTTCAGGTAGAAAATCCGA 1051 0 761 .......................................................................................... 850 K K W S A E T P Y L Y T L R A T L K D G S Q A S E V V P V K Query_1 1052 AAAAATGGAGCGCAGAAACTCCTTACTTATACACACTGCGTGCTACACTGAAAGACGGTAGCCAGGCTAGCGAAGTTGTACCTGTGAAAG 1141 0 851 .......................................................................................... 940 V G F R K I E L K N A Q I L V N G Q P V L F K G A D R H E L Query_1 1142 TAGGTTTCCGCAAGATTGAACTCAAAAACGCACAGATTCTGGTCAACGGCCAGCCTGTACTTTTCAAAGGTGCCGACCGTCATGAACTGG 1231 0 941 .......................................................................................... 1030 D P D G G Y V V S R D R M I Q D I Q I M K Q F N I N A V R T Query_1 1232 ACCCGGACGGTGGATACGTAGTTTCACGCGACCGCATGATTCAGGACATTCAGATTATGAAGCAGTTCAACATCAACGCCGTAAGAACCT 1321 0 1031 .......................................................................................... 1120 C H Y P D D N F W Y E L C D K Y G I Y V V A E A N I E S H G Query_1 1322 GTCACTATCCGGACGACAACTTCTGGTACGAACTCTGCGACAAATACGGTATTTATGTAGTGGCTGAAGCCAACATCGAATCTCACGGAA 1411 0 1121 .......................................................................................... 1210 0 2183 ...........T... 2197 1 936 ...........T... 922 M G Y G D K T L A K E P T Y K K A H M E R N Q R N V Q R G F Query_1 1412 TGGGATACGGCGACAAGACACTGGCCAAGGAACCGACTTATAAAAAGGCACACATGGAACGCAACCAGCGCAACGTACAGCGTGGTTTCA 1501 0 1211 .......................................................................................... 1300 0 262 ............ 273 N H P S I I F W S L G N E A G Y G P N F E A A Y D W I K A E Query_1 1502 ACCATCCGAGCATCATCTTCTGGTCATTGGGTAACGAGGCCGGATACGGTCCGAACTTCGAAGCTGCTTACGACTGGATCAAGGCAGAAG 1591 0 1301 .......................................................................................... 1390 D P S R A V Q Y E Q A R I E G K T D I Y C P M Y A N Y K W C Query_1 1592 ACCCCAGCCGTGCCGTACAATACGAACAGGCCCGCATCGAAGGAAAGACTGATATCTACTGCCCGATGTATGCCAACTACAAATGGTGCG 1681 0 1391 .......................................................................................... 1480 E E Y S K D D K Y Q K P L I Q C A Y A H A M G N S Q G G F K Query_1 1682 AAGAATACAGCAAGGACGATAAATACCAGAAACCGCTGATTCAGTGTGCTTATGCACACGCCATGGGTAATTCACAAGGTGGATTCAAGG 1771 0 1481 ................................................AA........................................ 1570 E Y W D L I R K Y P K Y Q G G F I W D F V D Q S I R W T G K Query_1 1772 AATACTGGGACCTGATTCGCAAGTATCCGAAATATCAGGGTGGATTCATCTGGGACTTCGTAGACCAGTCTATCCGCTGGACAGGAAAGA 1861 0 1571 .......................................................................................... 1660 N G K M I Y A Y A G D F N R M D D S G D K N F C N N G L I S Query_1 1862 ACGGCAAGATGATTTATGCTTATGCCGGCGACTTCAACCGTATGGATGATTCTGGCGACAAGAACTTCTGTAACAACGGACTTATCAGCC 1951 0 1661 .......................................................................................... 1750 P D R K P N P H A Y E V Q Y F Y Q N I W T T P G D L A K G E Query_1 1952 CTGACCGTAAACCGAATCCGCATGCATACGAAGTACAGTACTTCTACCAGAACATCTGGACTACTCCGGGCGATTTGGCAAAGGGAGAAA 2041 0 1751 .......................................................................................... 1840 I K V Y N E N F F R D L S A Y A L E W E V L K D G K A I R S Query_1 2042 TCAAGGTATACAACGAAAACTTCTTCCGTGACCTGTCTGCATACGCACTGGAATGGGAAGTACTGAAAGATGGTAAGGCTATCCGTAGCG 2131 0 1841 .......................................................................................... 1930 1 1215 ..A...NN......N......N.NNNT--....... 1182 G R I E N L N V A P H Q T A S L K L D L G K T C Q C G E W L Query_1 2132 GACGCATTGAAAACCTGAACGTGGCACCTCATCAGACTGCCAGCCTGAAACTTGATCTGGGC-AAGACTTGCCAGTGTGGTGAATGGCTG 2220 0 1931 ..............................................................-........................... 2019 1 1181 .NGC.N.G....--.....NC.NNN...NN.......N......T.NNNC--....N.....C..........CAGNNN.......-... 1097 2 506 ..G.........-.. 519 L N V K Y I Q K N R E G L I P A G F V V A K D Q L V L N P Query_1 2221 CTCAACGTGAAATACATCCAGAAGAACCGTGAAGGTTTGATCCCGGCCGGTTTTGTAGTAGCTAAA-GACCAGCTTGTGCTGAATCCATA 2309 0 2020 ..................................................................-....................... 2108 1 1096 ..................AN.......-.........-..N.......CNN...............AN...................... 1009 Y K A P A M E L K N V V E T N Q Q V V E P Q I N P N Y P D Y Query_1 2310 CAAAGCACCGGCTATGGAACTGAAGAACGTAGTTGAAACTAACCAGCAGGTAGTTGAACCGCAAATCAACCCGAACTATCCGGACTACAT 2399 0 2109 .......................................................................................... 2198 0 1124 ...........G... 1138 1 1008 ...................N.............-....................-................................... 921 I T V E G D G F Y L E F S K R N G Y L D R Y D V N G V S M L Query_1 2400 CACTGTAGAA-GGCGACGGATTCTATCTGGAATTCAGCAAACGCAACGGTTATCTTGACAGATACGATGTAAACGGAGTAAGCATGTTGA 2488 0 2199 ..........-............................................................................... 2287 1 920 ..........A............................................................................... 831 K E G E A L T P N F W R A P T D N D F G A G L Q N K Y A A W Query_1 2489 AAGAAGGCGAAGCACTGACACCGAACTTCTGGCGTGCCCCGACCGACAACGACTTCGGTGCTGGTCTGCAGAACAAATATGCTGCATGGA 2578 0 2288 .......................................................................................... 2377 1 830 .......................................................................................... 741 K N P G I K L T D F N Y K V E N G Q A I V N S T Y D L K E V Query_1 2579 AGAACCCGGGCATCAAACTGACAGACTTCAACTACAAGGTAGAAAACGGACAGGCCATCGTAAACTCTACTTACGACCTCAAGGAAGTAT 2668 0 2378 .......................................................................................... 2467 1 740 .......................................................................................... 651 2 83 ........... 73 S A K L Y L T Y Q I N N Q G A V K V T Q K M V A D K N A K V Query_1 2669 CTGCCAAGTTGTATCTCACTTACCAGATCAACAACCAGGGAGCAGTGAAAGTGACACAGAAAATGGTAGCCGACAAGAATGCCAAGGTAT 2758 0 2468 .......................................................................................... 2557 1 650 .......................................................................................... 561 S P M F R F G M Q M V M P K S F E K I S Y Y G R G P I E N Y Query_1 2759 CACCGATGTTCCGTTTCGGTATGCAGATGGTAATGCCGAAGTCATTTGAAAAGATTTCTTACTACGGACGCGGTCCGATAGAAAACTACA 2848 0 2558 .......................................................................................... 2647 1 560 .......................................................................................... 471 S D R N H C T D L G I Y N Q N V S D Q F Y P Y I R P Q E N G Query_1 2849 GCGACCGTAACCATTGCACTGACCTGGGAATCTACAACCAGAATGTAAGCGACCAGTTCTATCCGTACATCCGTCCGCAGGAAAACGGCA 2938 0 2648 .......................................................................................... 2737 1 470 .......................................................................................... 381 N K T D I R W W K Q V N M A G N G L M F V A E A P F S A S A Query_1 2939 ACAAGACTGACATCCGCTGGTGGAAACAGGTAAACATGGCTGGAAACGGACTGATGTTCGTAGCTGAAGCACCGTTCTCTGCTTCTGCTC 3028 0 2738 .......................................................................................... 2827 1 380 .......................................................................................... 291 L H Y T I A S L D D G T E K K Q S H S F E V E E A D L T N L Query_1 3029 TCCACTACACCATCGCTTCACTGGACGACGGTACTGAAAAGAAACAGAGCCACTCGTTCGAAGTAGAAGAAGCCGACCTGACCAACCTGT 3118 0 2828 .......................................................................................... 2917 1 290 .......................................................................................... 201 L I D K A Q M G L G C V N S W G A L P E D K Y M L P Y G D Y Query_1 3119 TGATTGACAAGGCACAGATGGGCTTAGGTTGTGTCAACAGCTGGGGTGCCCTGCCGGAAGACAAGTACATGCTTCCTTACGGAGATTATG 3208 0 2918 .......................................................................................... 3007 1 200 .......................................................................................... 111 E F T F I M T P V K G Y I A H E * H W K W I T D P N S S S V Query_1 3209 AATTTACGTTCATCATGACTCCGGTGAAAGGCTATATCGCACACGAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCG 3298 0 3008 ............................................... 3054 1 110 ...................................................................--..... 39 D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 3299 ACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGC 3374 1 43 .........................-..... 14 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 18743127 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 5,501 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200133.106 (CMR200133.20-1037.P(1037).E465Q.pMCSG68)
QTFKEWQDPEINAINRAPMHANFFAYENAEMAAKAVKEDSKNFMSLNGTWKFFWVRNADARPTDFWKTGFNDKGWDNLQVPGVWETHGYGDPIYINVGYGWRSRFENNPPYVPTEENHVGSYRREIMVPADWKGKDIIAHFGAVSSNMYLWVNGKFVGYSEDSKLEAEFNLTPYLKPGQKNLIAFQVFRWCDGSYLEDQDFFRYTGVARDCYLYTRNKKRIDDIRVTPDLDSEYKNGSLAINLNLKGSGNVSLELLDAQNQTVASTEVKGSGNVSTTLQVENPKKWSAETPYLYTLRATLKDGSQASEVVPVKVGFRKIELKNAQILVNGQPVLFKGADRHELDPDGGYVVSRDRMIQDIQIMKQFNINAVRTCHYPDDNFWYELCDKYGIYVVAEANIESHGMGYGDKTLAKEPTYKKAHMERNQRNVQRGFNHPSIIFWSLGNEAGYGPNFEAAYDWIKAEDPSRAVQYEQARIEGKTDIYCPMYANYKWCEEYSKDDKYQKPLIQCEYAHAMGNSQGGFKEYWDLIRKYPKYQGGFIWDFVDQSIRWTGKNGKMIYAYAGDFNRMDDSGDKNFCNNGLISPDRKPNPHAYEVQYFYQNIWTTPGDLAKGEIKVYNENFFRDLSAYALEWEVLKDGKAIRSGRIENLNVAPHQTASLKLDLGKTCQCGEWLLNVKYIQKNREGLIPAGFVVAKDQLVLNPYKAPAMELKNVVETNQQVVEPQINPNYPDYITVEGDGFYLEFSKRNGYLDRYDVNGVSMLKEGEALTPNFWRAPTDNDFGAGLQNKYAAWKNPGIKLTDFNYKVENGQAIVNSTYDLKEVSAKLYLTYQINNQGAVKVTQKMVADKNAKVSPMFRFGMQMVMPKSFEKISYYGRGPIENYSDRNHCTDLGIYNQNVSDQFYPYIRPQENGNKTDIRWWKQVNMAGNGLMFVAEAPFSASALHYTIASLDDGTEKKQSHSFEVEEADLTNLLIDKAQMGLGCVNSWGALPEDKYMLPYGDYEFTFIMTPVKGYIAHE
CAGACATTTAAAGAATGGCAAGACCCGGAAATAAATGCCATCAACCGGGCACCGATGCACGCAAACTTCTTTGCGTACGAAAATGCAGAAATGGCTGCCAAAGCAGTCAAGGAAGACTCCAAGAACTTTATGAGCCTGAATGGAACCTGGAAATTCTTCTGGGTGCGGAATGCAGATGCACGCCCCACGGATTTCTGGAAAACCGGCTTCAACGATAAAGGTTGGGACAACCTTCAGGTGCCGGGAGTATGGGAAACTCACGGATACGGCGACCCTATCTACATCAACGTAGGCTACGGATGGCGCAGCCGTTTCGAGAACAATCCTCCTTACGTACCGACTGAAGAAAACCACGTAGGTTCTTACCGTCGTGAAATTATGGTTCCGGCCGACTGGAAAGGCAAAGACATCATCGCTCATTTTGGTGCAGTCAGCTCAAACATGTACCTGTGGGTAAACGGTAAGTTCGTTGGCTACAGCGAAGACAGCAAGCTGGAAGCTGAATTCAACCTGACTCCGTACCTGAAACCGGGACAGAAAAACCTGATTGCTTTCCAGGTGTTCCGCTGGTGTGACGGTTCATACCTGGAAGACCAGGACTTCTTCCGCTATACCGGAGTAGCACGCGACTGCTATCTTTATACCCGCAACAAAAAACGTATCGATGACATCCGCGTGACTCCCGACCTGGACAGCGAATACAAGAACGGTTCACTGGCCATCAACCTGAACCTGAAAGGAAGCGGCAATGTCAGCCTGGAACTGCTGGATGCCCAAAACCAGACCGTAGCTTCTACCGAAGTAAAAGGTTCAGGCAATGTTTCTACTACACTTCAGGTAGAAAATCCGAAAAAATGGAGCGCAGAAACTCCTTACTTATACACACTGCGTGCTACACTGAAAGACGGTAGCCAGGCTAGCGAAGTTGTACCTGTGAAAGTAGGTTTCCGCAAGATTGAACTCAAAAACGCACAGATTCTGGTCAACGGCCAGCCTGTACTTTTCAAAGGTGCCGACCGTCATGAACTGGACCCGGACGGTGGATACGTAGTTTCACGCGACCGCATGATTCAGGACATTCAGATTATGAAGCAGTTCAACATCAACGCCGTAAGAACCTGTCACTATCCGGACGACAACTTCTGGTACGAACTCTGCGACAAATACGGTATTTATGTAGTGGCTGAAGCCAACATCGAATCTCACGGAATGGGATACGGCGACAAGACACTGGCCAAGGAACCGACTTATAAAAAGGCACACATGGAACGCAACCAGCGCAACGTACAGCGTGGTTTCAACCATCCGAGCATCATCTTCTGGTCATTGGGTAACGAGGCCGGATACGGTCCGAACTTCGAAGCTGCTTACGACTGGATCAAGGCAGAAGACCCCAGCCGTGCCGTACAATACGAACAGGCCCGCATCGAAGGAAAGACTGATATCTACTGCCCGATGTATGCCAACTACAAATGGTGCGAAGAATACAGCAAGGACGATAAATACCAGAAACCGCTGATTCAGTGTGAATATGCACACGCCATGGGTAATTCACAAGGTGGATTCAAGGAATACTGGGACCTGATTCGCAAGTATCCGAAATATCAGGGTGGATTCATCTGGGACTTCGTAGACCAGTCTATCCGCTGGACAGGAAAGAACGGCAAGATGATTTATGCTTATGCCGGCGACTTCAACCGTATGGATGATTCTGGCGACAAGAACTTCTGTAACAACGGACTTATCAGCCCTGACCGTAAACCGAATCCGCATGCATACGAAGTACAGTACTTCTACCAGAACATCTGGACTACTCCGGGCGATTTGGCAAAGGGAGAAATCAAGGTATACAACGAAAACTTCTTCCGTGACCTGTCTGCATACGCACTGGAATGGGAAGTACTGAAAGATGGTAAGGCTATCCGTAGCGGACGCATTGAAAACCTGAACGTGGCACCTCATCAGACTGCCAGCCTGAAACTTGATCTGGGCAAGACTTGCCAGTGTGGTGAATGGCTGCTCAACGTGAAATACATCCAGAAGAACCGTGAAGGTTTGATCCCGGCCGGTTTTGTAGTAGCTAAAGACCAGCTTGTGCTGAATCCATACAAAGCACCGGCTATGGAACTGAAGAACGTAGTTGAAACTAACCAGCAGGTAGTTGAACCGCAAATCAACCCGAACTATCCGGACTACATCACTGTAGAAGGCGACGGATTCTATCTGGAATTCAGCAAACGCAACGGTTATCTTGACAGATACGATGTAAACGGAGTAAGCATGTTGAAAGAAGGCGAAGCACTGACACCGAACTTCTGGCGTGCCCCGACCGACAACGACTTCGGTGCTGGTCTGCAGAACAAATATGCTGCATGGAAGAACCCGGGCATCAAACTGACAGACTTCAACTACAAGGTAGAAAACGGACAGGCCATCGTAAACTCTACTTACGACCTCAAGGAAGTATCTGCCAAGTTGTATCTCACTTACCAGATCAACAACCAGGGAGCAGTGAAAGTGACACAGAAAATGGTAGCCGACAAGAATGCCAAGGTATCACCGATGTTCCGTTTCGGTATGCAGATGGTAATGCCGAAGTCATTTGAAAAGATTTCTTACTACGGACGCGGTCCGATAGAAAACTACAGCGACCGTAACCATTGCACTGACCTGGGAATCTACAACCAGAATGTAAGCGACCAGTTCTATCCGTACATCCGTCCGCAGGAAAACGGCAACAAGACTGACATCCGCTGGTGGAAACAGGTAAACATGGCTGGAAACGGACTGATGTTCGTAGCTGAAGCACCGTTCTCTGCTTCTGCTCTCCACTACACCATCGCTTCACTGGACGACGGTACTGAAAAGAAACAGAGCCACTCGTTCGAAGTAGAAGAAGCCGACCTGACCAACCTGTTGATTGACAAGGCACAGATGGGCTTAGGTTGTGTCAACAGCTGGGGTGCCCTGCCGGAAGACAAGTACATGCTTCCTTACGGAGATTATGAATTTACGTTCATCATGACTCCGGTGAAAGGCTATATCGCACACGAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,558 total letters Query= CMR200133.106 Length=3459 Score E Sequences producing significant alignments: (Bits) Value 0_wt 5503 0.0 1_06A_A9_CMR200133.106_T7term.seq 1847 0.0 2_09A_A9_CMR200133.106_T7S.seq 89.7 2e-20 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 57 ATTCCCCTCTAGAAA-TAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGT 145 2 16 ...............A.....................................A.. 71 W S H P Q F E K G T E N L Y F Q S N A Q T F K E W Q D P E I Query_1 146 GGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAGACATTTAAAGAATGGCAAGACCCGGAAATAA 235 0 1 .................................. 34 N A I N R A P M H A N F F A Y E N A E M A A K A V K E D S K Query_1 236 ATGCCATCAACCGGGCACCGATGCACGCAAACTTCTTTGCGTACGAAAATGCAGAAATGGCTGCCAAAGCAGTCAAGGAAGACTCCAAGA 325 0 35 .......................................................................................... 124 N F M S L N G T W K F F W V R N A D A R P T D F W K T G F N Query_1 326 ACTTTATGAGCCTGAATGGAACCTGGAAATTCTTCTGGGTGCGGAATGCAGATGCACGCCCCACGGATTTCTGGAAAACCGGCTTCAACG 415 0 125 .......................................................................................... 214 D K G W D N L Q V P G V W E T H G Y G D P I Y I N V G Y G W Query_1 416 ATAAAGGTTGGGACAACCTTCAGGTGCCGGGAGTATGGGAAACTCACGGATACGGCGACCCTATCTACATCAACGTAGGCTACGGATGGC 505 0 215 .......................................................................................... 304 0 1213 ............ 1224 R S R F E N N P P Y V P T E E N H V G S Y R R E I M V P A D Query_1 506 GCAGCCGTTTCGAGAACAATCCTCCTTACGTACCGACTGAAGAAAACCACGTAGGTTCTTACCGTCGTGAAATTATGGTTCCGGCCGACT 595 0 305 .......................................................................................... 394 W K G K D I I A H F G A V S S N M Y L W V N G K F V G Y S E Query_1 596 GGAAAGGCAAAGACATCATCGCTCATTTTGGTGCAGTCAGCTCAAACATGTACCTGTGGGTAAACGGTAAGTTCGTTGGCTACAGCGAAG 685 0 395 .......................................................................................... 484 D S K L E A E F N L T P Y L K P G Q K N L I A F Q V F R W C Query_1 686 ACAGCAAGCTGGAAGCTGAATTCAACCTGACTCCGTACCTGAAACCGGGACAGAAAAACCTGATTGCTTTCCAGGTGTTCCGCTGGTGTG 775 0 485 .......................................................................................... 574 D G S Y L E D Q D F F R Y T G V A R D C Y L Y T R N K K R I Query_1 776 ACGGTTCATACCTGGAAGACCAGGACTTCTTCCGCTATACCGGAGTAGCACGCGACTGCTATCTTTATACCCGCAACAAAAAACGTATCG 865 0 575 .......................................................................................... 664 2 785 ....G.T.......... 801 D D I R V T P D L D S E Y K N G S L A I N L N L K G S G N V Query_1 866 ATGACATCCGCGTGACTCCCGACCTGGACAGCGAATACAAGAACGGTTCACTGGCCATCAACCTGAACCTGAAAGGAAGCGGCAATGTCA 955 0 665 .......................................................................................... 754 2 802 .. 803 S L E L L D A Q N Q T V A S T E V K G S G N V S T T L Q V E Query_1 956 GCCTGGAACTGCTGGATGCCCAAAACCAGACCGTAGCTTCTACCGAAGTAAAAGGTTCAGGCAATGTTTCTACTACACTTCAGGTAGAAA 1045 0 755 .......................................................................................... 844 N P K K W S A E T P Y L Y T L R A T L K D G S Q A S E V V P Query_1 1046 ATCCGAAAAAATGGAGCGCAGAAACTCCTTACTTATACACACTGCGTGCTACACTGAAAGACGGTAGCCAGGCTAGCGAAGTTGTACCTG 1135 0 845 .......................................................................................... 934 V K V G F R K I E L K N A Q I L V N G Q P V L F K G A D R H Query_1 1136 TGAAAGTAGGTTTCCGCAAGATTGAACTCAAAAACGCACAGATTCTGGTCAACGGCCAGCCTGTACTTTTCAAAGGTGCCGACCGTCATG 1225 0 935 .......................................................................................... 1024 E L D P D G G Y V V S R D R M I Q D I Q I M K Q F N I N A V Query_1 1226 AACTGGACCCGGACGGTGGATACGTAGTTTCACGCGACCGCATGATTCAGGACATTCAGATTATGAAGCAGTTCAACATCAACGCCGTAA 1315 0 1025 .......................................................................................... 1114 R T C H Y P D D N F W Y E L C D K Y G I Y V V A E A N I E S Query_1 1316 GAACCTGTCACTATCCGGACGACAACTTCTGGTACGAACTCTGCGACAAATACGGTATTTATGTAGTGGCTGAAGCCAACATCGAATCTC 1405 0 1115 .......................................................................................... 1204 0 2183 ...........T... 2197 1 934 ...........T... 920 H G M G Y G D K T L A K E P T Y K K A H M E R N Q R N V Q R Query_1 1406 ACGGAATGGGATACGGCGACAAGACACTGGCCAAGGAACCGACTTATAAAAAGGCACACATGGAACGCAACCAGCGCAACGTACAGCGTG 1495 0 1205 .......................................................................................... 1294 0 262 ............ 273 G F N H P S I I F W S L G N Q A G Y G P N F E A A Y D W I K Query_1 1496 GTTTCAACCATCCGAGCATCATCTTCTGGTCATTGGGTAACCAGGCCGGATACGGTCCGAACTTCGAAGCTGCTTACGACTGGATCAAGG 1585 0 1295 .........................................G................................................ 1384 A E D P S R A V Q Y E Q A R I E G K T D I Y C P M Y A N Y K Query_1 1586 CAGAAGACCCCAGCCGTGCCGTACAATACGAACAGGCCCGCATCGAAGGAAAGACTGATATCTACTGCCCGATGTATGCCAACTACAAAT 1675 0 1385 .......................................................................................... 1474 W C E E Y S K D D K Y Q K P L I Q C E Y A H A M G N S Q G G Query_1 1676 GGTGCGAAGAATACAGCAAGGACGATAAATACCAGAAACCGCTGATTCAGTGTGAATATGCACACGCCATGGGTAATTCACAAGGTGGAT 1765 0 1475 .......................................................................................... 1564 F K E Y W D L I R K Y P K Y Q G G F I W D F V D Q S I R W T Query_1 1766 TCAAGGAATACTGGGACCTGATTCGCAAGTATCCGAAATATCAGGGTGGATTCATCTGGGACTTCGTAGACCAGTCTATCCGCTGGACAG 1855 0 1565 .......................................................................................... 1654 G K N G K M I Y A Y A G D F N R M D D S G D K N F C N N G L Query_1 1856 GAAAGAACGGCAAGATGATTTATGCTTATGCCGGCGACTTCAACCGTATGGATGATTCTGGCGACAAGAACTTCTGTAACAACGGACTTA 1945 0 1655 .......................................................................................... 1744 I S P D R K P N P H A Y E V Q Y F Y Q N I W T T P G D L A K Query_1 1946 TCAGCCCTGACCGTAAACCGAATCCGCATGCATACGAAGTACAGTACTTCTACCAGAACATCTGGACTACTCCGGGCGATTTGGCAAAGG 2035 0 1745 .......................................................................................... 1834 G E I K V Y N E N F F R D L S A Y A L E W E V L K D G K A I Query_1 2036 GAGAAATCAAGGTATACAACGAAAACTTCTTCCGTGACCTGTCTGCATACGCACTGGAATGGGAAGTACTGAAAGATGGTAAGGCTATCC 2125 0 1835 .......................................................................................... 1924 R S G R I E N L N V A P H Q T A S L K L D L G K T C Q C G E Query_1 2126 GTAGCGGACGCATTGAAAACCTGAACGTGGCACCTCATCAGACTGCCAGCCTGAAACTTGATCTGGGCAAGACTTGCCAG-TGTGGTGAA 2214 0 1925 ................................................................................-......... 2013 1 1121 ...G.....G.AN...... 1103 2 519 ..G........... 532 W L L N V K Y I Q K N R E G L I P A G F V V A K D Q L V L Query_1 2215 TGGCTGCTCAACGTGAAA-TACATCCAGAAGAACCGTGAAGGTTTGATCCCGGCCGGTTTTGTAGTAGCTAAAGACCAGCTTGTGCTGAA 2303 0 2014 ..................-....................................................................... 2102 1 1102 AT........N.......A.....T......NN.......N...-..N..-....................................... 1015 N P Y K A P A M E L K N V V E T N Q Q V V E P Q I N P N Y P Query_1 2304 T-CCATACAAAGCACCGGCTATGGAACTGAAGAACGTAGTTGAAACTAACCAGCAGGTAGTTGAACCGCAAATCAACCCGAACTATCCGG 2392 0 2103 .-........................................................................................ 2191 0 1124 ......... 1132 1 1014 .T...........N...............................................-............................ 926 D Y I T V E G D G F Y L E F S K R N G Y L D R Y D V N G V S Query_1 2393 ACTACATCACTGTAGAAGGCGACGGATTCTATCTGGAATTCAGCAAACGCAACGGTTATCTTGACAGATACGATGTAAACGGAGTAAGCA 2482 0 2192 .......................................................................................... 2281 0 1133 ..G... 1138 1 925 .......................................................................................... 836 1 1010 ............ 1021 M L K E G E A L T P N F W R A P T D N D F G A G L Q N K Y A Query_1 2483 TGTTGAAAGAAGGCGAAGCACTGACACCGAACTTCTGGCGTGCCCCGACCGACAACGACTTCGGTGCTGGTCTGCAGAACAAATATGCTG 2572 0 2282 .......................................................................................... 2371 1 835 ...-...................................................................................... 747 A W K N P G I K L T D F N Y K V E N G Q A I V N S T Y D L K Query_1 2573 CATGGAAGAACCCGGGCATCAAACTGACAGACTTCAACTACAAGGTAGAAAACGGACAGGCCATCGTAAACTCTACTTACGACCTCAAGG 2662 0 2372 .......................................................................................... 2461 1 746 .......................................................................................... 657 2 96 ........... 86 E V S A K L Y L T Y Q I N N Q G A V K V T Q K M V A D K N A Query_1 2663 AAGTATCTGCCAAGTTGTATCTCACTTACCAGATCAACAACCAGGGAGCAGTGAAAGTGACACAGAAAATGGTAGCCGACAAGAATGCCA 2752 0 2462 .......................................................................................... 2551 1 656 .......................................................................................... 567 K V S P M F R F G M Q M V M P K S F E K I S Y Y G R G P I E Query_1 2753 AGGTATCACCGATGTTCCGTTTCGGTATGCAGATGGTAATGCCGAAGTCATTTGAAAAGATTTCTTACTACGGACGCGGTCCGATAGAAA 2842 0 2552 .......................................................................................... 2641 1 566 .......................................................................................... 477 N Y S D R N H C T D L G I Y N Q N V S D Q F Y P Y I R P Q E Query_1 2843 ACTACAGCGACCGTAACCATTGCACTGACCTGGGAATCTACAACCAGAATGTAAGCGACCAGTTCTATCCGTACATCCGTCCGCAGGAAA 2932 0 2642 .......................................................................................... 2731 1 476 .......................................................................................... 387 N G N K T D I R W W K Q V N M A G N G L M F V A E A P F S A Query_1 2933 ACGGCAACAAGACTGACATCCGCTGGTGGAAACAGGTAAACATGGCTGGAAACGGACTGATGTTCGTAGCTGAAGCACCGTTCTCTGCTT 3022 0 2732 .......................................................................................... 2821 1 386 .......................................................................................... 297 S A L H Y T I A S L D D G T E K K Q S H S F E V E E A D L T Query_1 3023 CTGCTCTCCACTACACCATCGCTTCACTGGACGACGGTACTGAAAAGAAACAGAGCCACTCGTTCGAAGTAGAAGAAGCCGACCTGACCA 3112 0 2822 .......................................................................................... 2911 1 296 .......................................................................................... 207 N L L I D K A Q M G L G C V N S W G A L P E D K Y M L P Y G Query_1 3113 ACCTGTTGATTGACAAGGCACAGATGGGCTTAGGTTGTGTCAACAGCTGGGGTGCCCTGCCGGAAGACAAGTACATGCTTCCTTACGGAG 3202 0 2912 .......................................................................................... 3001 1 206 .......................................................................................... 117 D Y E F T F I M T P V K G Y I A H E * H W K W I T D P N S S Query_1 3203 ATTATGAATTTACGTTCATCATGACTCCGGTGAAAGGCTATATCGCACACGAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCT 3292 0 3002 ..................................................... 3054 1 116 .........................................................................--..... 39 S V D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 3293 CCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGC 3374 1 43 ..........................A.... 13 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 18939264 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 5,558 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200133.107 (CMR200133.20-1037.P(1037).E529Q.pMCSG68)
QTFKEWQDPEINAINRAPMHANFFAYENAEMAAKAVKEDSKNFMSLNGTWKFFWVRNADARPTDFWKTGFNDKGWDNLQVPGVWETHGYGDPIYINVGYGWRSRFENNPPYVPTEENHVGSYRREIMVPADWKGKDIIAHFGAVSSNMYLWVNGKFVGYSEDSKLEAEFNLTPYLKPGQKNLIAFQVFRWCDGSYLEDQDFFRYTGVARDCYLYTRNKKRIDDIRVTPDLDSEYKNGSLAINLNLKGSGNVSLELLDAQNQTVASTEVKGSGNVSTTLQVENPKKWSAETPYLYTLRATLKDGSQASEVVPVKVGFRKIELKNAQILVNGQPVLFKGADRHELDPDGGYVVSRDRMIQDIQIMKQFNINAVRTCHYPDDNFWYELCDKYGIYVVAEANIESHGMGYGDKTLAKEPTYKKAHMERNQRNVQRGFNHPSIIFWSLGNEAGYGPNFEAAYDWIKAEDPSRAVQYEQARIEGKTDIYCPMYANYKWCEEYSKDDKYQKPLIQCEYAHAMGNSQGGFKEYWDLIRKYPKYQGGFIWDFVDQSIRWTGKNGKMIYAYAGDFNRMDDSGDKNFCNNGLISPDRKPNPHAYEVQYFYQNIWTTPGDLAKGEIKVYNENFFRDLSAYALEWEVLKDGKAIRSGRIENLNVAPHQTASLKLDLGKTCQCGEWLLNVKYIQKNREGLIPAGFVVAKDQLVLNPYKAPAMELKNVVETNQQVVEPQINPNYPDYITVEGDGFYLEFSKRNGYLDRYDVNGVSMLKEGEALTPNFWRAPTDNDFGAGLQNKYAAWKNPGIKLTDFNYKVENGQAIVNSTYDLKEVSAKLYLTYQINNQGAVKVTQKMVADKNAKVSPMFRFGMQMVMPKSFEKISYYGRGPIENYSDRNHCTDLGIYNQNVSDQFYPYIRPQENGNKTDIRWWKQVNMAGNGLMFVAEAPFSASALHYTIASLDDGTEKKQSHSFEVEEADLTNLLIDKAQMGLGCVNSWGALPEDKYMLPYGDYEFTFIMTPVKGYIAHE
CAGACATTTAAAGAATGGCAAGACCCGGAAATAAATGCCATCAACCGGGCACCGATGCACGCAAACTTCTTTGCGTACGAAAATGCAGAAATGGCTGCCAAAGCAGTCAAGGAAGACTCCAAGAACTTTATGAGCCTGAATGGAACCTGGAAATTCTTCTGGGTGCGGAATGCAGATGCACGCCCCACGGATTTCTGGAAAACCGGCTTCAACGATAAAGGTTGGGACAACCTTCAGGTGCCGGGAGTATGGGAAACTCACGGATACGGCGACCCTATCTACATCAACGTAGGCTACGGATGGCGCAGCCGTTTCGAGAACAATCCTCCTTACGTACCGACTGAAGAAAACCACGTAGGTTCTTACCGTCGTGAAATTATGGTTCCGGCCGACTGGAAAGGCAAAGACATCATCGCTCATTTTGGTGCAGTCAGCTCAAACATGTACCTGTGGGTAAACGGTAAGTTCGTTGGCTACAGCGAAGACAGCAAGCTGGAAGCTGAATTCAACCTGACTCCGTACCTGAAACCGGGACAGAAAAACCTGATTGCTTTCCAGGTGTTCCGCTGGTGTGACGGTTCATACCTGGAAGACCAGGACTTCTTCCGCTATACCGGAGTAGCACGCGACTGCTATCTTTATACCCGCAACAAAAAACGTATCGATGACATCCGCGTGACTCCCGACCTGGACAGCGAATACAAGAACGGTTCACTGGCCATCAACCTGAACCTGAAAGGAAGCGGCAATGTCAGCCTGGAACTGCTGGATGCCCAAAACCAGACCGTAGCTTCTACCGAAGTAAAAGGTTCAGGCAATGTTTCTACTACACTTCAGGTAGAAAATCCGAAAAAATGGAGCGCAGAAACTCCTTACTTATACACACTGCGTGCTACACTGAAAGACGGTAGCCAGGCTAGCGAAGTTGTACCTGTGAAAGTAGGTTTCCGCAAGATTGAACTCAAAAACGCACAGATTCTGGTCAACGGCCAGCCTGTACTTTTCAAAGGTGCCGACCGTCATGAACTGGACCCGGACGGTGGATACGTAGTTTCACGCGACCGCATGATTCAGGACATTCAGATTATGAAGCAGTTCAACATCAACGCCGTAAGAACCTGTCACTATCCGGACGACAACTTCTGGTACGAACTCTGCGACAAATACGGTATTTATGTAGTGGCTGAAGCCAACATCGAATCTCACGGAATGGGATACGGCGACAAGACACTGGCCAAGGAACCGACTTATAAAAAGGCACACATGGAACGCAACCAGCGCAACGTACAGCGTGGTTTCAACCATCCGAGCATCATCTTCTGGTCATTGGGTAACGAGGCCGGATACGGTCCGAACTTCGAAGCTGCTTACGACTGGATCAAGGCAGAAGACCCCAGCCGTGCCGTACAATACGAACAGGCCCGCATCGAAGGAAAGACTGATATCTACTGCCCGATGTATGCCAACTACAAATGGTGCGAAGAATACAGCAAGGACGATAAATACCAGAAACCGCTGATTCAGTGTGAATATGCACACGCCATGGGTAATTCACAAGGTGGATTCAAGGAATACTGGGACCTGATTCGCAAGTATCCGAAATATCAGGGTGGATTCATCTGGGACTTCGTAGACCAGTCTATCCGCTGGACAGGAAAGAACGGCAAGATGATTTATGCTTATGCCGGCGACTTCAACCGTATGGATGATTCTGGCGACAAGAACTTCTGTAACAACGGACTTATCAGCCCTGACCGTAAACCGAATCCGCATGCATACGAAGTACAGTACTTCTACCAGAACATCTGGACTACTCCGGGCGATTTGGCAAAGGGAGAAATCAAGGTATACAACGAAAACTTCTTCCGTGACCTGTCTGCATACGCACTGGAATGGGAAGTACTGAAAGATGGTAAGGCTATCCGTAGCGGACGCATTGAAAACCTGAACGTGGCACCTCATCAGACTGCCAGCCTGAAACTTGATCTGGGCAAGACTTGCCAGTGTGGTGAATGGCTGCTCAACGTGAAATACATCCAGAAGAACCGTGAAGGTTTGATCCCGGCCGGTTTTGTAGTAGCTAAAGACCAGCTTGTGCTGAATCCATACAAAGCACCGGCTATGGAACTGAAGAACGTAGTTGAAACTAACCAGCAGGTAGTTGAACCGCAAATCAACCCGAACTATCCGGACTACATCACTGTAGAAGGCGACGGATTCTATCTGGAATTCAGCAAACGCAACGGTTATCTTGACAGATACGATGTAAACGGAGTAAGCATGTTGAAAGAAGGCGAAGCACTGACACCGAACTTCTGGCGTGCCCCGACCGACAACGACTTCGGTGCTGGTCTGCAGAACAAATATGCTGCATGGAAGAACCCGGGCATCAAACTGACAGACTTCAACTACAAGGTAGAAAACGGACAGGCCATCGTAAACTCTACTTACGACCTCAAGGAAGTATCTGCCAAGTTGTATCTCACTTACCAGATCAACAACCAGGGAGCAGTGAAAGTGACACAGAAAATGGTAGCCGACAAGAATGCCAAGGTATCACCGATGTTCCGTTTCGGTATGCAGATGGTAATGCCGAAGTCATTTGAAAAGATTTCTTACTACGGACGCGGTCCGATAGAAAACTACAGCGACCGTAACCATTGCACTGACCTGGGAATCTACAACCAGAATGTAAGCGACCAGTTCTATCCGTACATCCGTCCGCAGGAAAACGGCAACAAGACTGACATCCGCTGGTGGAAACAGGTAAACATGGCTGGAAACGGACTGATGTTCGTAGCTGAAGCACCGTTCTCTGCTTCTGCTCTCCACTACACCATCGCTTCACTGGACGACGGTACTGAAAAGAAACAGAGCCACTCGTTCGAAGTAGAAGAAGCCGACCTGACCAACCTGTTGATTGACAAGGCACAGATGGGCTTAGGTTGTGTCAACAGCTGGGGTGCCCTGCCGGAAGACAAGTACATGCTTCCTTACGGAGATTATGAATTTACGTTCATCATGACTCCGGTGAAAGGCTATATCGCACACGAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,526 total letters Query= CMR200133.107 Length=3459 Score E Sequences producing significant alignments: (Bits) Value 0_wt 5499 0.0 1_06B_B9_CMR200133.107_T7term.seq 1829 0.0 2_09B_B9_CMR200133.107_T7S.seq 87.8 7e-20 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 57 ATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTG 146 2 13 ..............-.....................................A.. 66 W S H P Q F E K G T E N L Y F Q S N A Q T F K E W Q D P E I Query_1 147 GTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCAGACATTTAAAGAATGGCAAGACCCGGAAATAAA 236 0 1 ................................... 35 N A I N R A P M H A N F F A Y E N A E M A A K A V K E D S K Query_1 237 TGCCATCAACCGGGCACCGATGCACGCAAACTTCTTTGCGTACGAAAATGCAGAAATGGCTGCCAAAGCAGTCAAGGAAGACTCCAAGAA 326 0 36 .......................................................................................... 125 N F M S L N G T W K F F W V R N A D A R P T D F W K T G F N Query_1 327 CTTTATGAGCCTGAATGGAACCTGGAAATTCTTCTGGGTGCGGAATGCAGATGCACGCCCCACGGATTTCTGGAAAACCGGCTTCAACGA 416 0 126 .......................................................................................... 215 D K G W D N L Q V P G V W E T H G Y G D P I Y I N V G Y G W Query_1 417 TAAAGGTTGGGACAACCTTCAGGTGCCGGGAGTATGGGAAACTCACGGATACGGCGACCCTATCTACATCAACGTAGGCTACGGATGGCG 506 0 216 .......................................................................................... 305 0 1213 ............ 1224 R S R F E N N P P Y V P T E E N H V G S Y R R E I M V P A D Query_1 507 CAGCCGTTTCGAGAACAATCCTCCTTACGTACCGACTGAAGAAAACCACGTAGGTTCTTACCGTCGTGAAATTATGGTTCCGGCCGACTG 596 0 306 .......................................................................................... 395 W K G K D I I A H F G A V S S N M Y L W V N G K F V G Y S E Query_1 597 GAAAGGCAAAGACATCATCGCTCATTTTGGTGCAGTCAGCTCAAACATGTACCTGTGGGTAAACGGTAAGTTCGTTGGCTACAGCGAAGA 686 0 396 .......................................................................................... 485 D S K L E A E F N L T P Y L K P G Q K N L I A F Q V F R W C Query_1 687 CAGCAAGCTGGAAGCTGAATTCAACCTGACTCCGTACCTGAAACCGGGACAGAAAAACCTGATTGCTTTCCAGGTGTTCCGCTGGTGTGA 776 0 486 .......................................................................................... 575 D G S Y L E D Q D F F R Y T G V A R D C Y L Y T R N K K R I Query_1 777 CGGTTCATACCTGGAAGACCAGGACTTCTTCCGCTATACCGGAGTAGCACGCGACTGCTATCTTTATACCCGCAACAAAAAACGTATCGA 866 0 576 .......................................................................................... 665 2 780 ....G.T........... 797 D D I R V T P D L D S E Y K N G S L A I N L N L K G S G N V Query_1 867 TGACATCCGCGTGACTCCCGACCTGGACAGCGAATACAAGAACGGTTCACTGGCCATCAACCTGAACCTGAAAGGAAGCGGCAATGTCAG 956 0 666 .......................................................................................... 755 2 798 . 798 S L E L L D A Q N Q T V A S T E V K G S G N V S T T L Q V E Query_1 957 CCTGGAACTGCTGGATGCCCAAAACCAGACCGTAGCTTCTACCGAAGTAAAAGGTTCAGGCAATGTTTCTACTACACTTCAGGTAGAAAA 1046 0 756 .......................................................................................... 845 N P K K W S A E T P Y L Y T L R A T L K D G S Q A S E V V P Query_1 1047 TCCGAAAAAATGGAGCGCAGAAACTCCTTACTTATACACACTGCGTGCTACACTGAAAGACGGTAGCCAGGCTAGCGAAGTTGTACCTGT 1136 0 846 .......................................................................................... 935 V K V G F R K I E L K N A Q I L V N G Q P V L F K G A D R H Query_1 1137 GAAAGTAGGTTTCCGCAAGATTGAACTCAAAAACGCACAGATTCTGGTCAACGGCCAGCCTGTACTTTTCAAAGGTGCCGACCGTCATGA 1226 0 936 .......................................................................................... 1025 E L D P D G G Y V V S R D R M I Q D I Q I M K Q F N I N A V Query_1 1227 ACTGGACCCGGACGGTGGATACGTAGTTTCACGCGACCGCATGATTCAGGACATTCAGATTATGAAGCAGTTCAACATCAACGCCGTAAG 1316 0 1026 .......................................................................................... 1115 R T C H Y P D D N F W Y E L C D K Y G I Y V V A E A N I E S Query_1 1317 AACCTGTCACTATCCGGACGACAACTTCTGGTACGAACTCTGCGACAAATACGGTATTTATGTAGTGGCTGAAGCCAACATCGAATCTCA 1406 0 1116 .......................................................................................... 1205 0 2183 ...........T... 2197 1 934 ...........T... 920 H G M G Y G D K T L A K E P T Y K K A H M E R N Q R N V Q R Query_1 1407 CGGAATGGGATACGGCGACAAGACACTGGCCAAGGAACCGACTTATAAAAAGGCACACATGGAACGCAACCAGCGCAACGTACAGCGTGG 1496 0 1206 .......................................................................................... 1295 0 262 ............ 273 G F N H P S I I F W S L G N E A G Y G P N F E A A Y D W I K Query_1 1497 TTTCAACCATCCGAGCATCATCTTCTGGTCATTGGGTAACGAGGCCGGATACGGTCCGAACTTCGAAGCTGCTTACGACTGGATCAAGGC 1586 0 1296 .......................................................................................... 1385 A E D P S R A V Q Y E Q A R I E G K T D I Y C P M Y A N Y K Query_1 1587 AGAAGACCCCAGCCGTGCCGTACAATACGAACAGGCCCGCATCGAAGGAAAGACTGATATCTACTGCCCGATGTATGCCAACTACAAATG 1676 0 1386 .......................................................................................... 1475 W C E E Y S K D D K Y Q K P L I Q C Q Y A H A M G N S Q G G Query_1 1677 GTGCGAAGAATACAGCAAGGACGATAAATACCAGAAACCGCTGATTCAGTGTCAGTATGCACACGCCATGGGTAATTCACAAGGTGGATT 1766 0 1476 ....................................................G.A................................... 1565 F K E Y W D L I R K Y P K Y Q G G F I W D F V D Q S I R W T Query_1 1767 CAAGGAATACTGGGACCTGATTCGCAAGTATCCGAAATATCAGGGTGGATTCATCTGGGACTTCGTAGACCAGTCTATCCGCTGGACAGG 1856 0 1566 .......................................................................................... 1655 G K N G K M I Y A Y A G D F N R M D D S G D K N F C N N G L Query_1 1857 AAAGAACGGCAAGATGATTTATGCTTATGCCGGCGACTTCAACCGTATGGATGATTCTGGCGACAAGAACTTCTGTAACAACGGACTTAT 1946 0 1656 .......................................................................................... 1745 I S P D R K P N P H A Y E V Q Y F Y Q N I W T T P G D L A K Query_1 1947 CAGCCCTGACCGTAAACCGAATCCGCATGCATACGAAGTACAGTACTTCTACCAGAACATCTGGACTACTCCGGGCGATTTGGCAAAGGG 2036 0 1746 .......................................................................................... 1835 G E I K V Y N E N F F R D L S A Y A L E W E V L K D G K A I Query_1 2037 AGAAATCAAGGTATACAACGAAAACTTCTTCCGTGACCTGTCTGCATACGCACTGGAATGGGAAG-TACTGAAAGATGGT-AAGGCTATC 2124 0 1836 .................................................................-..............-......... 1923 1 1210 ......A....TN.....N..T......... 1180 R S G R I E N L N V A P H Q T A S L K L D L G K T C Q C G Query_1 2125 CGTAGCGGACGCATTGAAAACCTGAACGT-GGCACCTCATCAGACTGCCAGCCTGAAACTTGATCTGGGCAAGACTTGCCAGTGTGGTGA 2213 0 1924 .............................-............................................................ 2012 1 1179 .......NN.NTN------.N...NNN..T..........TCNNN..........N.N--.....N.N--...N..-..........-.T 1102 2 514 ..G........... 527 E W L L N V K Y I Q K N R E G L I P A G F V V A K D Q L V L Query_1 2214 ATGGCTGCTCAACGTGAAA-TACA-TCCAGAAGAACCGTGAAGGTTTGATCCCGGCCGGTTTTGTAGTAGCTAAAGACCAGCTTGTGCTG 2301 0 2013 ...................-....-................................................................. 2100 1 1101 GNN....N...........AN.N.A........NN--.........-.......N...N...-...........-.N......-...... 1018 N P Y K A P A M E L K N V V E T N Q Q V V E P Q I N P N Y Query_1 2302 AATCCATACAAAGCACCGGCTATGGAACTGAAGAACGTAGTTGAAACTAA-CCAGCAGGTAGTTGAACCGCAAATCAACCCGAACTATCC 2390 0 2101 ..................................................-....................................... 2189 0 1124 ....... 1130 1 1017 ......NT.N.......................................NN....................................... 928 P D Y I T V E G D G F Y L E F S K R N G Y L D R Y D V N G V Query_1 2391 GGACTACATCACTGTAGAAGGCGACGGATTCTATCTGGAATTCAGCAAACGCAACGGTTATCTTGACAGATACGATGTAAACGGAGTAAG 2480 0 2190 .......................................................................................... 2279 0 1131 ....G... 1138 1 927 ...............................................................-.......................... 839 S M L K E G E A L T P N F W R A P T D N D F G A G L Q N K Y Query_1 2481 CATGTTGAAAGAAGGCGAAGCACTGACACCGAACTTCTGGCGTGCCCCGACCGACAACGACTTCGGTGCTGGTCTGCAGAACAAATATGC 2570 0 2280 .......................................................................................... 2369 1 838 .......................................................................................... 749 A A W K N P G I K L T D F N Y K V E N G Q A I V N S T Y D L Query_1 2571 TGCATGGAAGAACCCGGGCATCAAACTGACAGACTTCAACTACAAGGTAGAAAACGGACAGGCCATCGTAAACTCTACTTACGACCTCAA 2660 0 2370 .......................................................................................... 2459 1 748 .......................................................................................... 659 2 91 ........... 81 K E V S A K L Y L T Y Q I N N Q G A V K V T Q K M V A D K N Query_1 2661 GGAAGTATCTGCCAAGTTGTATCTCACTTACCAGATCAACAACCAGGGAGCAGTGAAAGTGACACAGAAAATGGTAGCCGACAAGAATGC 2750 0 2460 .......................................................................................... 2549 1 658 .......................................................................................... 569 A K V S P M F R F G M Q M V M P K S F E K I S Y Y G R G P I Query_1 2751 CAAGGTATCACCGATGTTCCGTTTCGGTATGCAGATGGTAATGCCGAAGTCATTTGAAAAGATTTCTTACTACGGACGCGGTCCGATAGA 2840 0 2550 .......................................................................................... 2639 1 568 .......................................................................................... 479 E N Y S D R N H C T D L G I Y N Q N V S D Q F Y P Y I R P Q Query_1 2841 AAACTACAGCGACCGTAACCATTGCACTGACCTGGGAATCTACAACCAGAATGTAAGCGACCAGTTCTATCCGTACATCCGTCCGCAGGA 2930 0 2640 .......................................................................................... 2729 1 478 .......................................................................................... 389 E N G N K T D I R W W K Q V N M A G N G L M F V A E A P F S Query_1 2931 AAACGGCAACAAGACTGACATCCGCTGGTGGAAACAGGTAAACATGGCTGGAAACGGACTGATGTTCGTAGCTGAAGCACCGTTCTCTGC 3020 0 2730 .......................................................................................... 2819 1 388 .......................................................................................... 299 A S A L H Y T I A S L D D G T E K K Q S H S F E V E E A D L Query_1 3021 TTCTGCTCTCCACTACACCATCGCTTCACTGGACGACGGTACTGAAAAGAAACAGAGCCACTCGTTCGAAGTAGAAGAAGCCGACCTGAC 3110 0 2820 .......................................................................................... 2909 1 298 .......................................................................................... 209 T N L L I D K A Q M G L G C V N S W G A L P E D K Y M L P Y Query_1 3111 CAACCTGTTGATTGACAAGGCACAGATGGGCTTAGGTTGTGTCAACAGCTGGGGTGCCCTGCCGGAAGACAAGTACATGCTTCCTTACGG 3200 0 2910 .......................................................................................... 2999 1 208 .......................................................................................... 119 G D Y E F T F I M T P V K G Y I A H E * H W K W I T D P N S Query_1 3201 AGATTATGAATTTACGTTCATCATGACTCCGGTGAAAGGCTATATCGCACACGAATAACATTGGAAGTGGATAACGGATCCGAATTCGAG 3290 0 3000 ....................................................... 3054 1 118 ...........................................................................--..... 39 S S V D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 3291 CTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAA 3372 1 43 .........................A... 15 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 18829152 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 5,526 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200137.120 (CMR200137.19-931.P(931).E435A.pMCSG68)
QTDKIDLAGSWTFSTDSMDWSRVIELPGSMASNGFGEDIAVGTDWTGGIVDSSYFFKPSYAKYREAGNIKVPFWLQPVKYYKGKAWYQKEVVIPDSWEGKDISLFLERCHWESRLYIDGKEIGMQNALGAPHRYDLTGKLSAGKHVLMLCVDNRVKNIDPGENSHSISDHTQGNWNGVVGDMFLEVKPEVNVSSVKIMPERLAKKVSVSASLMNRYEKDANVVLEMTVGNEKVQQQCTLKPGENQVMMSLAMKGDIKCWDEFSPSLYDLKLSVKDADSGETDVYAERFGFRDVKVKDGKLTINDRRLFLRGTLDCAVFPKTGFPPTDVESWKKIYTTCRQHGLNHVRFHSWCPPEAAFAAADGMGMYLEIECSSWANQSTTIGDGGDLDRFIWEESERIVREFGNHPSFCMMMYGNEPAGEGSNAYLTNFVTTWKERDARRLYCSGAGWPNLPVNDFLSDSNPRIQAWGQGVKSIINAQAPRTDYDWSEYIGRFQQPMVSHEIGQWCVYPNFKEMAKYDGVMRPRNFEIFQETLAENGMAHLADSFLLASGKLQALCYKADIEAALRTKDFGGFQLLGLSDFPGQGTALVGVLDAFWEEKGYIRPEEYRRFCNSTVPLLRLPKLIYTNQETVKGSLEVAHFGAAPLEVTSTVWTLKTKEGKTIASGTLAHQPVGIGNCIPLGQLEIPLDKVDVPSCLTLEATLGDYANSWHIWVYPAAVQKVADEAQLLMTDRLDAKALQRLQEGGNVLLSLRKGSLPAEAGGEVVIGFSSIFWNTAWTLGQAPHTLGILCNPAHPALSEFPTEYYSDYQWWDAMSHSGAIEVVKIDKNLQPIVRVIDDWFTNRPLALLFEVKVGKGKLLVSGIDFWQDMDKRTEARQLLYSLKKYMCGNRFNPSSEVDAKDLSILFSIKNQK
CAGACTGATAAGATTGACCTGGCCGGCTCGTGGACATTTTCTACGGACAGCATGGACTGGAGCCGGGTGATTGAACTGCCGGGTTCAATGGCTTCCAATGGTTTTGGGGAAGATATTGCCGTGGGTACTGATTGGACGGGCGGTATTGTGGATTCTTCTTATTTCTTTAAACCTTCGTATGCCAAATACCGTGAGGCAGGAAATATCAAGGTACCTTTCTGGCTTCAGCCGGTAAAATATTACAAGGGTAAGGCGTGGTATCAGAAAGAGGTGGTGATTCCGGACAGTTGGGAAGGAAAGGACATTTCTCTCTTTTTGGAACGATGCCATTGGGAGAGCCGTTTGTATATAGACGGAAAGGAAATCGGCATGCAAAATGCTTTGGGGGCGCCCCATCGTTATGACCTGACAGGCAAGCTTTCAGCAGGGAAACATGTGTTGATGCTGTGTGTAGACAATCGGGTGAAAAACATTGATCCGGGGGAGAACTCACATAGTATTTCCGACCATACACAAGGAAACTGGAACGGGGTGGTAGGCGATATGTTCCTGGAAGTAAAGCCGGAAGTGAATGTGTCTTCCGTCAAGATTATGCCGGAGCGTCTGGCTAAGAAAGTCAGTGTGTCGGCTTCCTTGATGAACCGTTATGAAAAAGATGCCAATGTGGTACTGGAGATGACGGTAGGTAATGAAAAAGTACAGCAACAATGTACGTTGAAGCCGGGCGAAAATCAAGTGATGATGTCGCTGGCCATGAAGGGAGACATTAAGTGCTGGGATGAGTTTTCTCCATCCTTATATGATTTGAAGCTGAGTGTGAAGGATGCGGATAGCGGTGAAACGGATGTCTATGCGGAACGTTTTGGTTTCCGTGATGTGAAGGTGAAAGACGGCAAACTCACCATCAACGACCGCCGTTTGTTCCTGCGTGGTACGCTGGATTGTGCCGTATTTCCGAAGACCGGTTTCCCGCCCACGGATGTAGAATCCTGGAAAAAGATTTATACCACCTGTCGGCAGCACGGACTGAACCATGTGCGCTTCCATTCCTGGTGTCCGCCCGAAGCTGCTTTTGCAGCTGCCGATGGGATGGGTATGTACCTGGAGATAGAATGTTCTTCCTGGGCTAACCAGTCGACTACCATTGGCGATGGAGGCGATCTGGACCGCTTTATCTGGGAGGAAAGTGAACGCATCGTCCGTGAGTTTGGTAACCATCCTTCTTTCTGCATGATGATGTACGGTAACGAACCGGCTGGTGAGGGAAGTAATGCCTATCTGACTAATTTTGTTACTACCTGGAAAGAGCGCGATGCCCGCCGTTTATATTGTTCGGGTGCCGGATGGCCCAATTTGCCGGTTAACGACTTCTTGAGCGATTCCAATCCTCGTATTCAGGCGTGGGGACAAGGTGTGAAGAGTATTATCAACGCACAGGCTCCGCGTACCGACTATGACTGGTCAGAATACATCGGACGTTTCCAGCAGCCGATGGTGAGCCACGAAATCGGGCAGTGGTGTGTATATCCCAACTTCAAGGAAATGGCCAAATACGACGGGGTGATGCGCCCGCGTAATTTTGAGATATTCCAGGAAACACTGGCTGAAAACGGTATGGCACATTTGGCTGACAGCTTCCTGCTGGCTTCCGGAAAATTGCAGGCGTTGTGTTATAAGGCCGATATCGAAGCTGCTTTGCGTACAAAAGACTTCGGTGGATTCCAGTTACTGGGCTTGTCTGATTTCCCGGGGCAGGGTACGGCTTTGGTAGGAGTGCTCGATGCGTTCTGGGAAGAAAAAGGCTACATCCGTCCGGAAGAATACCGTCGTTTCTGTAATAGTACGGTACCATTACTGCGCTTGCCGAAGTTGATTTATACCAACCAGGAAACGGTGAAAGGAAGTCTGGAAGTGGCACATTTCGGAGCTGCTCCGCTGGAGGTGACTTCTACTGTCTGGACCCTGAAAACAAAAGAAGGAAAGACAATTGCTTCGGGCACGCTGGCACACCAGCCGGTAGGTATCGGCAATTGTATTCCGTTGGGGCAGCTGGAGATTCCATTGGATAAGGTGGACGTCCCTTCATGTCTGACACTGGAAGCTACATTGGGAGATTACGCCAACAGCTGGCACATCTGGGTATATCCTGCTGCGGTACAGAAAGTAGCTGATGAAGCACAATTGCTGATGACCGACCGTCTGGATGCAAAAGCTTTGCAACGTCTTCAGGAAGGTGGCAACGTACTGCTTTCTTTACGGAAAGGCTCCTTGCCTGCCGAAGCGGGAGGCGAAGTAGTGATAGGTTTCTCTAGCATCTTCTGGAACACGGCCTGGACGCTGGGACAAGCACCGCACACACTGGGTATCCTGTGTAACCCCGCTCATCCGGCACTTTCAGAGTTCCCTACAGAGTATTACAGTGATTATCAGTGGTGGGATGCCATGAGCCATTCCGGTGCCATCGAAGTGGTCAAGATTGATAAAAACTTGCAGCCGATTGTACGAGTTATCGACGACTGGTTTACGAACCGTCCGCTGGCTTTGTTGTTCGAAGTGAAGGTGGGTAAGGGTAAATTGCTTGTGTCAGGAATTGATTTCTGGCAGGATATGGACAAGCGTACGGAAGCCCGTCAGTTACTCTACAGCTTGAAGAAATATATGTGCGGTAATCGCTTCAATCCCTCTTCTGAAGTCGATGCGAAAGATTTAAGTATTTTGTTTTCCATTAAAAATCAAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,116 total letters Query= CMR200137.120 Length=3144 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4931 0.0 1_01A_A4_CMR200137.120_ST7term.seq 1903 0.0 2_04A_CMR200137.120Seq171A04.seq 376 8e-107 Q T D K I D L A G S W T F S T D S M D W S R V I E L P G S M Query_1 202 CAGACTGATAAGATTGACCTGGCCGGCTCGTGGACATTTTCTACGGACAGCATGGACTGGAGCCGGGTGATTGAACTGCCGGGTTCAATG 291 0 1 .......................................................................................... 90 A S N G F G E D I A V G T D W T G G I V D S S Y F F K P S Y Query_1 292 GCTTCCAATGGTTTTGGGGAAGATATTGCCGTGGGTACTGATTGGACGGGCGGTATTGTGGATTCTTCTTATTTCTTTAAACCTTCGTAT 381 0 91 .......................................................................................... 180 A K Y R E A G N I K V P F W L Q P V K Y Y K G K A W Y Q K E Query_1 382 GCCAAATACCGTGAGGCAGGAAATATCAAGGTACCTTTCTGGCTTCAGCCGGTAAAATATTACAAGGGTAAGGCGTGGTATCAGAAAGAG 471 0 181 .......................................................................................... 270 V V I P D S W E G K D I S L F L E R C H W E S R L Y I D G K Query_1 472 GTGGTGATTCCGGACAGTTGGGAAGGAAAGGACATTTCTCTCTTTTTGGAACGATGCCATTGGGAGAGCCGTTTGTATATAGACGGAAAG 561 0 271 .......................................................................................... 360 E I G M Q N A L G A P H R Y D L T G K L S A G K H V L M L C Query_1 562 GAAATCGGCATGCAAAATGCTTTGGGGGCGCCCCATCGTTATGACCTGACAGGCAAGCTTTCAGCAGGGAAACATGTGTTGATGCTGTGT 651 0 361 .......................................................................................... 450 V D N R V K N I D P G E N S H S I S D H T Q G N W N G V V G Query_1 652 GTAGACAATCGGGTGAAAAACATTGATCCGGGGGAGAACTCACATAGTATTTCCGACCATACACAAGGAAACTGGAACGGGGTGGTAGGC 741 0 451 .......................................................................................... 540 2 822 ....C..N.T........N..A..T..N.TC.A.N.G..C..CT 779 D M F L E V K P E V N V S S V K I M P E R L A K K V S V S Query_1 742 GATATGTTCCTGGAAGTAAAGCCGG-AAGTGAATGTGTCTTCCGTCAAGATTATGCCGGAGCGTCTGGCTAAG-AAAGTCAGTGTGTCGG 829 0 541 .........................-...............................................-................ 628 2 778 ....A.N.NNN.A.NT.......T.CN...C.CC........G........A..-.AA.T.N...AA..GC..T....G..CN....... 690 2 117 ............ 128 A S L M N R Y E K D A N V V L E M T V G N E K V Q Q Q C T Query_1 830 CTTCCTTGATGAACC-GTTATGAAAAAGATGCCAATGTGGTACTGGAGATGACGGTAGGTAATGAAAAAGTACAG---CAACAATGTACG 915 0 629 ...............-...........................................................---............ 714 2 689 N.C.GAN.....N.GT......C..........T..CNTT.TTC.T.CCA.....C.T....CA.TC....N..AAGC....CC..CC.- 601 L K P G E N Q V M M S L A M K G D I K C W D E F S P S L Y D Query_1 916 TTGAAGCCGGGCGAAAATCAAGTGATGATGTCGCTGGCCATGAAGGGAGACATTAAGTGCTGGGATGAGTTTTCTCCATCCTTATATGAT 1005 0 715 .......................................................................................... 804 2 600 ---......AT.AG.C.A.......A.....NATCC...GN..T.T.N.G...GC.AA..A...........GNC..T..AAC.C..... 514 L K L S V K D A D S G E T D V Y A E R F G F R D V K V K D Query_1 1006 TTGAAGCTGAGTGTGAAGGATGC-GGATAGCGGTGAAACGGATGTCTATGCGGAACGTTTTGGTTTCCGTGATGTGAAGGTGAAAGACGG 1094 0 805 .......................-.................................................................. 893 2 513 N..C.AT..TCC.-....CCA..A...AG...TC...CAAA........N...TT.TC...CAG...TC-..G.N......C....TT.. 426 G K L T I N D R R L F L R G T L D C A V F P K T G F P P Query_1 1095 CAAA-CTCACCATCAACGACCGCCGTTTGTTCCTG--CGTGGTACGCTGG--ATTGTGCCGTATTTCCGAAGACCG-GTTTCCCGCCCAC 1178 0 894 ....-..............................--.............--........................-............. 977 2 425 ...GG.G..T..C.C-..T....N--...NC.A.TTC....--.A.T...GA.A.NCA.N.C..........N...T.......-.ATCG 342 T D V E S W K K I Y T T C R Q H G L N H V R F H S W C P P E Query_1 1179 GGATGTAGAATCCTGGAAAAAGATTTATACCACCTGTCGGCAGCACGGACTGAACCATGTGCGCTTCCATTCCTGGTGTCCGCCCGAAGC 1268 0 978 .......................................................................................... 1067 0 1686 ...... 1691 0 1872 ........... 1882 1 999 ........... 989 2 341 ...N..G.....N.....TGTA......C.....ATAG......CG..GAG.....G... 282 A A F A A A D G M G M Y L E I E C S S W A N Q S T T I G D G Query_1 1269 TGCTTTTGCAGCTGCCGATGGGATGGGTATGTACCTGGAGATAGAATGTTCTTCCTGGGCTAACCAGTCGACTACCATTGGCGATGGAGG 1358 0 1068 .......................................................................................... 1157 0 1692 ...... 1697 2 2 ....-..... 10 2 218 ............. 206 G D L D R F I W E E S E R I V R E F G N H P S F C M M M Y G Query_1 1359 CGATCTGGACCGCTTTATCTGGGAGGAAAGTGAACGCATCGTCCGTGAGTTTGGTAACCATCCTTCTTTCTGCATGATGATGTACGGTAA 1448 0 1158 .......................................................................................... 1247 2 11 .........................................................G..........G....T.............G.. 100 N A P A G E G S N A Y L T N F V T T W K E R D A R R L Y C S Query_1 1449 CGCTCCGGCTGGTGAGGGAAGTAATGCCTATCTGACTAATTTTGTTACTACCTGGAAAGAGCGCGATGCCCGCCGTTTATATTGTTCGGG 1538 0 1248 ..AA...................................................................................... 1337 2 101 ....G...................A............C..............G......................-.GG.....NA.T.. 189 G A G W P N L P V N D F L S D S N P R I Q A W G Q G V K S I Query_1 1539 TGCCGGATGGCCCAATTTGCCGGTTAACGACTTCTTGAGCGATTCCAATCCTCGTATTCAGGCGTGGGGACAAGGTGTGAAGAGTATTAT 1628 0 1338 .......................................................................................... 1427 2 190 ..................CTATC.CC.G...C..AC.CAT....T.N....CT.C.AA.GN.....C....G.....C...A.TG..... 279 I N A Q A P R T D Y D W S E Y I G R F Q Q P M V S H E I G Q Query_1 1629 CAACGCACAGGCTCCGCG--TACCGACTATGACTGGTCAGAATACATCGGACGTTTCCAGCAGCCGATGGTGAGCCACGAAATCGGGCAG 1716 0 1428 ..................--...................................................................... 1515 2 280 ....A.GGTTC....C..GC.G..--.....GTG..AT.A.T.....TCC.N.A.....CNTC.......G..AA....GNC.TC..A.A 367 W C V Y P N F K E M A K Y D G V M R P R N F E I F Q E T L A Query_1 1717 TGGTGTGTATATCCCAACTTCAAGGAAATGGCCAAATACGACGGGGTGATGCGCCCGCGTAATTTTGAGATATTCCAGGAAACACTGGCT 1806 0 1516 .......................................................................................... 1605 2 368 ..CN...N..T...........C........N...NGCG................TTGCC..C.....CC.TCN..TC..G.-....AAA 456 E N G M A H L A D S F L L A S G K L Q A L C Y K A D I E A Query_1 1807 GAAAACGGTATGGCACATTTGGCT-GACAGCTTCCTGCTGGCTTCCGGAAAATTGCAGGCGTTGTGTTATAAGGCCGATATCGAAGCTGC 1895 0 1606 ........................-................................................................. 1694 0 1062 ......... 1070 1 1183 ... 1181 2 457 ..G...C.N..A.-......T.T.C...GC...................C.......NATCA......GA.G..N.A.AC..ATCC.... 545 A L R T K D F G G F Q L L G L S D F P G Q G T A L V G V L Query_1 1896 TTTGCGTACAAAAGACTT-CGGTGGATTCCA--GTTACTGGGCTTGTCTGATTTCCCGGGGCAGGGTACGGCTTTGG-TAGGAGTGCTCG 1981 0 1695 ..................-............--............................................-............ 1780 0 1071 ... 1073 1 1180 N.N....TAC........T....T....N..--..N.....-..A........N......-..A......-...G.T-A........NN. 1097 2 546 .....A.G.CNC.C.TCN-...C....NA..TCT.C...T.--.........CGG.TT.........-T.......NACTT..T..T.AC 631 D A F W E E K G Y I R P E E Y R R F C N S T V P L L R L Query_1 1982 ATGCG-TTCTGGGAAG--AAAAAGGCTACAT-CCGTCC-GGAAGAATACCGTCGTTTCTGT-AATAGTACGGTACCATTACTGCGC-TTG 2064 0 1781 .....-..........--.............-......-......................-........................-... 1863 1 1096 ..N..A....T....AGA.............T......C......................N..........N.....-.......C... 1008 2 632 ....C-G.....T.C.--.....N.A.. 656 2 695 ..N..G..T.........-... 715 P K L I Y T N Q E T V K G S L E V A H F G A A P L E V T S T Query_1 2065 CCGAAGTTGATTTATACCAACCAGGAAACGGTGAAAGGAAGTCTGGAAGTGGCACATTTCGGAGCTGCTCCGCTGGAGGTGACTTCTACT 2154 0 1864 .......................................................................................... 1953 0 999 ........... 1009 1 1007 ....................................A..............-......CG.A............................ 919 2 716 A.NC.C...... 727 V W T L K T K E G K T I A S G T L A H Q P V G I G N C I P L Query_1 2155 GTCTGGACCCTGAAAACAAAAGAAGGAAAGACAATTGCTTCGGGCACGCTGGCACACCAGCCGGTAGGTATCGGCAATTGTATTCCGTTG 2244 0 1954 .......................................................................................... 2043 1 918 ..................................N....................................................... 829 G Q L E I P L D K V D V P S C L T L E A T L G D Y A N S W H Query_1 2245 GGGCAGCTGGAGATTCCATTGGATAAGGTGGACGTCCCTTCATGTCTGACACTGGAAGCTACATTGGGAGATTACGCCAACAGCTGGCAC 2334 0 2044 .......................................................................................... 2133 1 828 .......................................................................................... 739 I W V Y P A A V Q K V A D E A Q L L M T D R L D A K A L Q R Query_1 2335 ATCTGGGTATATCCTGCTGCGGTACAGAAAGTAGCTGATGAAGCACAATTGCTGATGACCGACCGTCTGGATGCAAAAGCTTTGCAACGT 2424 0 2134 .......................................................................................... 2223 1 738 .......................................................................................... 649 L Q E G G N V L L S L R K G S L P A E A G G E V V I G F S S Query_1 2425 CTTCAGGAAGGTGGCAACGTACTGCTTTCTTTACGGAAAGGCTCCTTGCCTGCCGAAGCGGGAGGCGAAGTAGTGATAGGTTTCTCTAGC 2514 0 2224 .......................................................................................... 2313 1 648 .......................................................................................... 559 I F W N T A W T L G Q A P H T L G I L C N P A H P A L S E F Query_1 2515 ATCTTCTGGAACACGGCCTGGACGCTGGGACAAGCACCGCACACACTGGGTATCCTGTGTAACCCCGCTCATCCGGCACTTTCAGAGTTC 2604 0 2314 .......................................................................................... 2403 1 558 .......................................................................................... 469 P T E Y Y S D Y Q W W D A M S H S G A I E V V K I D K N L Q Query_1 2605 CCTACAGAGTATTACAGTGATTATCAGTGGTGGGATGCCATGAGCCATTCCGGTGCCATCGAAGTGGTCAAGATTGATAAAAACTTGCAG 2694 0 2404 .......................................................................................... 2493 1 468 .......................................................................................... 379 P I V R V I D D W F T N R P L A L L F E V K V G K G K L L V Query_1 2695 CCGATTGTACGAGTTATCGACGACTGGTTTACGAACCGTCCGCTGGCTTTGTTGTTCGAAGTGAAGGTGGGTAAGGGTAAATTGCTTGTG 2784 0 2494 .......................................................................................... 2583 1 378 .......................................................................................... 289 S G I D F W Q D M D K R T E A R Q L L Y S L K K Y M C G N R Query_1 2785 TCAGGAATTGATTTCTGGCAGGATATGGACAAGCGTACGGAAGCCCGTCAGTTACTCTACAGCTTGAAGAAATATATGTGCGGTAATCGC 2874 0 2584 .......................................................................................... 2673 1 288 .......................................................................................... 199 F N P S S E V D A K D L S I L F S I K N Q K * H W K W I T D Query_1 2875 TTCAATCCCTCTTCTGAAGTCGATGCGAAAGATTTAAGTATTTTGTTTTCCATTAAAAATCAAAAATAACATTGGAAGTGGATAACGGAT 2964 0 2674 .................................................................. 2739 1 198 .......................................................................................... 109 P N S S S V D K L A A A L E H H H H H H * D P A A N K A R K Query_1 2965 CCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAG 3054 1 108 .............................N...........................................................A 19 E Query_1 3055 GAA 3057 1 18 ... 16 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 15823812 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 5,116 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200137.121 (CMR200137.19-931.P(931).E435Q.pMCSG68)
QTDKIDLAGSWTFSTDSMDWSRVIELPGSMASNGFGEDIAVGTDWTGGIVDSSYFFKPSYAKYREAGNIKVPFWLQPVKYYKGKAWYQKEVVIPDSWEGKDISLFLERCHWESRLYIDGKEIGMQNALGAPHRYDLTGKLSAGKHVLMLCVDNRVKNIDPGENSHSISDHTQGNWNGVVGDMFLEVKPEVNVSSVKIMPERLAKKVSVSASLMNRYEKDANVVLEMTVGNEKVQQQCTLKPGENQVMMSLAMKGDIKCWDEFSPSLYDLKLSVKDADSGETDVYAERFGFRDVKVKDGKLTINDRRLFLRGTLDCAVFPKTGFPPTDVESWKKIYTTCRQHGLNHVRFHSWCPPEAAFAAADGMGMYLEIECSSWANQSTTIGDGGDLDRFIWEESERIVREFGNHPSFCMMMYGNEPAGEGSNAYLTNFVTTWKERDARRLYCSGAGWPNLPVNDFLSDSNPRIQAWGQGVKSIINAQAPRTDYDWSEYIGRFQQPMVSHEIGQWCVYPNFKEMAKYDGVMRPRNFEIFQETLAENGMAHLADSFLLASGKLQALCYKADIEAALRTKDFGGFQLLGLSDFPGQGTALVGVLDAFWEEKGYIRPEEYRRFCNSTVPLLRLPKLIYTNQETVKGSLEVAHFGAAPLEVTSTVWTLKTKEGKTIASGTLAHQPVGIGNCIPLGQLEIPLDKVDVPSCLTLEATLGDYANSWHIWVYPAAVQKVADEAQLLMTDRLDAKALQRLQEGGNVLLSLRKGSLPAEAGGEVVIGFSSIFWNTAWTLGQAPHTLGILCNPAHPALSEFPTEYYSDYQWWDAMSHSGAIEVVKIDKNLQPIVRVIDDWFTNRPLALLFEVKVGKGKLLVSGIDFWQDMDKRTEARQLLYSLKKYMCGNRFNPSSEVDAKDLSILFSIKNQK
CAGACTGATAAGATTGACCTGGCCGGCTCGTGGACATTTTCTACGGACAGCATGGACTGGAGCCGGGTGATTGAACTGCCGGGTTCAATGGCTTCCAATGGTTTTGGGGAAGATATTGCCGTGGGTACTGATTGGACGGGCGGTATTGTGGATTCTTCTTATTTCTTTAAACCTTCGTATGCCAAATACCGTGAGGCAGGAAATATCAAGGTACCTTTCTGGCTTCAGCCGGTAAAATATTACAAGGGTAAGGCGTGGTATCAGAAAGAGGTGGTGATTCCGGACAGTTGGGAAGGAAAGGACATTTCTCTCTTTTTGGAACGATGCCATTGGGAGAGCCGTTTGTATATAGACGGAAAGGAAATCGGCATGCAAAATGCTTTGGGGGCGCCCCATCGTTATGACCTGACAGGCAAGCTTTCAGCAGGGAAACATGTGTTGATGCTGTGTGTAGACAATCGGGTGAAAAACATTGATCCGGGGGAGAACTCACATAGTATTTCCGACCATACACAAGGAAACTGGAACGGGGTGGTAGGCGATATGTTCCTGGAAGTAAAGCCGGAAGTGAATGTGTCTTCCGTCAAGATTATGCCGGAGCGTCTGGCTAAGAAAGTCAGTGTGTCGGCTTCCTTGATGAACCGTTATGAAAAAGATGCCAATGTGGTACTGGAGATGACGGTAGGTAATGAAAAAGTACAGCAACAATGTACGTTGAAGCCGGGCGAAAATCAAGTGATGATGTCGCTGGCCATGAAGGGAGACATTAAGTGCTGGGATGAGTTTTCTCCATCCTTATATGATTTGAAGCTGAGTGTGAAGGATGCGGATAGCGGTGAAACGGATGTCTATGCGGAACGTTTTGGTTTCCGTGATGTGAAGGTGAAAGACGGCAAACTCACCATCAACGACCGCCGTTTGTTCCTGCGTGGTACGCTGGATTGTGCCGTATTTCCGAAGACCGGTTTCCCGCCCACGGATGTAGAATCCTGGAAAAAGATTTATACCACCTGTCGGCAGCACGGACTGAACCATGTGCGCTTCCATTCCTGGTGTCCGCCCGAAGCTGCTTTTGCAGCTGCCGATGGGATGGGTATGTACCTGGAGATAGAATGTTCTTCCTGGGCTAACCAGTCGACTACCATTGGCGATGGAGGCGATCTGGACCGCTTTATCTGGGAGGAAAGTGAACGCATCGTCCGTGAGTTTGGTAACCATCCTTCTTTCTGCATGATGATGTACGGTAACGAACCGGCTGGTGAGGGAAGTAATGCCTATCTGACTAATTTTGTTACTACCTGGAAAGAGCGCGATGCCCGCCGTTTATATTGTTCGGGTGCCGGATGGCCCAATTTGCCGGTTAACGACTTCTTGAGCGATTCCAATCCTCGTATTCAGGCGTGGGGACAAGGTGTGAAGAGTATTATCAACGCACAGGCTCCGCGTACCGACTATGACTGGTCAGAATACATCGGACGTTTCCAGCAGCCGATGGTGAGCCACGAAATCGGGCAGTGGTGTGTATATCCCAACTTCAAGGAAATGGCCAAATACGACGGGGTGATGCGCCCGCGTAATTTTGAGATATTCCAGGAAACACTGGCTGAAAACGGTATGGCACATTTGGCTGACAGCTTCCTGCTGGCTTCCGGAAAATTGCAGGCGTTGTGTTATAAGGCCGATATCGAAGCTGCTTTGCGTACAAAAGACTTCGGTGGATTCCAGTTACTGGGCTTGTCTGATTTCCCGGGGCAGGGTACGGCTTTGGTAGGAGTGCTCGATGCGTTCTGGGAAGAAAAAGGCTACATCCGTCCGGAAGAATACCGTCGTTTCTGTAATAGTACGGTACCATTACTGCGCTTGCCGAAGTTGATTTATACCAACCAGGAAACGGTGAAAGGAAGTCTGGAAGTGGCACATTTCGGAGCTGCTCCGCTGGAGGTGACTTCTACTGTCTGGACCCTGAAAACAAAAGAAGGAAAGACAATTGCTTCGGGCACGCTGGCACACCAGCCGGTAGGTATCGGCAATTGTATTCCGTTGGGGCAGCTGGAGATTCCATTGGATAAGGTGGACGTCCCTTCATGTCTGACACTGGAAGCTACATTGGGAGATTACGCCAACAGCTGGCACATCTGGGTATATCCTGCTGCGGTACAGAAAGTAGCTGATGAAGCACAATTGCTGATGACCGACCGTCTGGATGCAAAAGCTTTGCAACGTCTTCAGGAAGGTGGCAACGTACTGCTTTCTTTACGGAAAGGCTCCTTGCCTGCCGAAGCGGGAGGCGAAGTAGTGATAGGTTTCTCTAGCATCTTCTGGAACACGGCCTGGACGCTGGGACAAGCACCGCACACACTGGGTATCCTGTGTAACCCCGCTCATCCGGCACTTTCAGAGTTCCCTACAGAGTATTACAGTGATTATCAGTGGTGGGATGCCATGAGCCATTCCGGTGCCATCGAAGTGGTCAAGATTGATAAAAACTTGCAGCCGATTGTACGAGTTATCGACGACTGGTTTACGAACCGTCCGCTGGCTTTGTTGTTCGAAGTGAAGGTGGGTAAGGGTAAATTGCTTGTGTCAGGAATTGATTTCTGGCAGGATATGGACAAGCGTACGGAAGCCCGTCAGTTACTCTACAGCTTGAAGAAATATATGTGCGGTAATCGCTTCAATCCCTCTTCTGAAGTCGATGCGAAAGATTTAAGTATTTTGTTTTCCATTAAAAATCAAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,038 total letters Query= CMR200137.121 Length=3144 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4931 0.0 1_01B_B4_CMR200137.121_T7term.seq 1817 0.0 2_04B_CMR200137.121Seq171B04.seq 279 2e-77 Q T D K I D L A G S W T F S T D S M D W S R V I E L P G S M Query_1 202 CAGACTGATAAGATTGACCTGGCCGGCTCGTGGACATTTTCTACGGACAGCATGGACTGGAGCCGGGTGATTGAACTGCCGGGTTCAATG 291 0 1 .......................................................................................... 90 A S N G F G E D I A V G T D W T G G I V D S S Y F F K P S Y Query_1 292 GCTTCCAATGGTTTTGGGGAAGATATTGCCGTGGGTACTGATTGGACGGGCGGTATTGTGGATTCTTCTTATTTCTTTAAACCTTCGTAT 381 0 91 .......................................................................................... 180 A K Y R E A G N I K V P F W L Q P V K Y Y K G K A W Y Q K E Query_1 382 GCCAAATACCGTGAGGCAGGAAATATCAAGGTACCTTTCTGGCTTCAGCCGGTAAAATATTACAAGGGTAAGGCGTGGTATCAGAAAGAG 471 0 181 .......................................................................................... 270 V V I P D S W E G K D I S L F L E R C H W E S R L Y I D G K Query_1 472 GTGGTGATTCCGGACAGTTGGGAAGGAAAGGACATTTCTCTCTTTTTGGAACGATGCCATTGGGAGAGCCGTTTGTATATAGACGGAAAG 561 0 271 .......................................................................................... 360 E I G M Q N A L G A P H R Y D L T G K L S A G K H V L M L C Query_1 562 GAAATCGGCATGCAAAATGCTTTGGGGGCGCCCCATCGTTATGACCTGACAGGCAAGCTTTCAGCAGGGAAACATGTGTTGATGCTGTGT 651 0 361 .......................................................................................... 450 2 899 .......N..NA....A.T...AT...N...N..NCN- 863 V D N R V K N I D P G E N S H S I S D H T Q G N W N G V V Query_1 652 GTAGACAA-TCGGGTGAAAAACATTGATCCGGGGGAGAACTCACATAGTATTTC-CGACCATACACAAGGAAACTGGAACGGGGTGGTAG 739 0 451 ........-.............................................-................................... 538 2 862 .....GGNG.........N.-...A...A.T..N.T.NC.....N.N....C..GN.T...G..........T.NNNTC.A...GA.C.. 774 G D M F L E V K P E V N V S S V K I M P E R L A K K V S V S Query_1 740 GCGATATGTTCCTGGAAGTAAAGCCGGAAGTGAATGTGTCTTCCGTCAAGATTATGCCGGAGCGTCTGGCTAAG-AAAGTCAGTGTGTCG 828 0 539 ..........................................................................-............... 627 2 773 CTN.G.NAA.GNGNC....CC.....C..............-.G....G......CNN......G.AA......T.......CN...... 685 A S L M N R Y E K D A N V V L E M T V G N E K V Q Q Q C T L Query_1 829 GCTTCCTTGATGAACCGTTATGAAAAAGATGCCAATGTGGTACTGGAGATGACGGTAGGTAATGAAAAAGTACAGCAACAATGTACGTTG 918 0 628 .......................................................................................... 717 2 684 AT.C.GNA....CGGT...N.TC.G....N...T.G.N...TT.....CA.........G.GC-..........AAGC............ 596 K P G E N Q V M M S L A M K G D I K C W D E F S P S L Y D Query_1 919 AAGCCGGGCGAAAATCAAGTGATGATGTCGCTGGCCATGAAG--GGAGACATTAAGTGCTGGGATGAGTTTTCTCCATCCTTATATGATT 1006 0 718 ..........................................--.............................................. 805 2 595 ....AAAT....C.....CA..N.....AATCCN..--....TG..T.G......AA..A...........GNC..TA.AAC.C.N.NCN 508 L K L S V K D A D S G E T D V Y A E R F G F R D V K V K D Query_1 1007 TGAAGCTGAGTGTGAAGGATGCGGATAGCGGTGAAACGGATGTCTATGCGGAACGTTTTGGTT---TCCGTGATGTGAAGGTGAAAGAC- 1092 0 806 ...............................................................---.......................- 891 2 507 ..C..T......G...C...CA...A....TC....A...G...C......----....CA.CCAG..NT...G.A-..T......A.TT 423 G K L T I N D R R L F L R G T L D C A V F P K T G F P P Query_1 1093 GGCAAACTCACCATCAAC-GACCGCCGTTTG---TTCCTGCGTGGTACGCTGG-AT-TGTGCCGTATTTCCGAAGACCGGTTTCCCGCCC 1176 0 892 ..................-............---...................-..-................................. 975 2 422 ........G--.....C.C.........G.CCAT.....---..--.A.T...G..A..CN.N.C..................T..C.AT 340 T D V E S W K K I Y T T C R Q H G L N H V R F H S W C P Query_1 1177 ACGGATGTAGAATCCTGGAAAAAGATTTATACCACCTGTCGGCAGCACGGACTGAACCATGT------GCGCTTCCATTCCTGGTGTCCG 1260 0 976 ..............................................................------...................... 1059 0 1872 ........... 1882 2 339 NG...G..G.....G.....TGT.......-...GTCA........-...GGA.CN......TGATAAT.AT..T.GCA...--...... 254 P E A A F A A A D G M G M Y L E I E C S S W A N Q S T T Query_1 1261 CCCGAAGCTGCTTTTGCAGCTGCCGA-TGGGATGGGT----ATGTACCTGGAGATAGAATGTTCTTCCT-GGGCTAACCAGTCGACTACC 1344 0 1060 ..........................-..........----............................-.................... 1143 0 1686 ............ 1697 2 253 .----..GC........----.AG..T...A...AN.CGAG.A..--.G..........AT.GG.C.A.CN...--......NC.A.... 176 I G D G G D L D R F I W E E S E R I V R E F G N H P S F C M Query_1 1345 ATTGGCGATGGAGGCGATCTGGACCGCTTTATCTGGGAGGAAAGTGAACGCATCGTCCGTGAGTTTGGTAACCATCCTTCTTTCTGCATG 1434 0 1144 .......................................................................................... 1233 2 5 .............................................................................T.. 84 2 175 ...........T..........N...G.. 147 M M Y G N Q P A G E G S N A Y L T N F V T T W K E R D A R R Query_1 1435 ATGATGTACGGTAACCAGCCGGCTGGTGAGGGAAGTAATGCCTATCTGACTAATTTTGTTACTACCTGGAAAGAGCGCGATGCCCGCCGT 1524 0 1234 ...............G.A........................................................................ 1323 2 85 ....G......G......G...A............A..A..........T.CC......AN.A...G.NCC...T...C.CCAT....AA 174 L Y C S G A G W P N L P V N D F L S D S N P R I Q A W G Q G Query_1 1525 TTATATTGTTCGGGTGCCGGATGGCCCAATTTGCCGGTTAACGACTTCTTGAGCGATTCCAATCCTCGTATTCAGGCGTGGGGACAAGGT 1614 0 1324 .......................................................................................... 1413 2 175 .GG.....NA.T......N.....A.......-.TATC.CC........C..NTC.............C.AAAGC..C..C......... 263 V K S I I N A Q A P R T D Y D W S E Y I G R F Q Q P M V S Query_1 1615 GTGAAGAGTATTATCAACGCACAG-GCTCCGCGTACCGACTATGACTGGTCAGAATACATCGGACGTTTCCAGCAGCCGATGGTGAGCCA 1703 0 1414 ........................-................................................................. 1502 2 264 .C...A.TG........--..TG.N.....C..-G.T.C.G........AT.A.TC....TCC...A.....C.TC..N....G..AAAC 350 H E I G Q W C V Y P N F K E M A K Y D G V M R P R N F E I Query_1 1704 CGAAATCGGGCAGTGGTGTGTATATCCCAACTTCAAGGAAATGGCCAAATACGACG---GGGTGATGC-GCCCGCGTAATTTTGAGATAT 1789 0 1503 ........................................................---.........-..................... 1588 2 351 ..GTC.TC..A.A..CN.N.C.......................----.C...G..GTC.........A.TTT..CA.T....C.C..-. 435 F Q E T L A E N G M A H L A D S F L L A S G K L Q A L C Y K Query_1 1790 TCCAGGAAACACTGGCTGAAAACGGTATGGCACATTTGGCTGACAGCTTCCTGCTGGCTTCCGGAAAATTGCAGGCGTTGTGTTATAAGG 1879 0 1589 .......................................................................................... 1678 2 436 ...-TC..NG.....A.......C.C.... 464 A D I E A A L R T K D F G G F Q L L G L S D F P G Q G T A Query_1 1880 CCGATATCGAAGCTGCTTTGCGTAC---AAAAGACTTCGGTGGATTCCAGTTACTGGGCTTGTCTGATTTCCCGGGGCAGGGTACGG-CT 1965 0 1679 .........................---...........................................................-.. 1764 0 1062 ............ 1073 1 1196 .............G.....TNCA..............A.NNN-..N-..NN.-..N..TNN.A.NN...N.NNN.N--....G.N 1117 L V G V L D A F W E E K G Y I R P E E Y R R F C N S T V P Query_1 1966 TTGGTAGGAGTGCTCGATGCGTTCTGGGAAG--AAAAAGGCTACATCCGTCCGGAAGAATA-CCGTCGTTTCTGTAATAGTACGGTACCA 2052 0 1765 ...............................--............................-............................ 1851 1 1116 .G....A.NAGN.........A........AGA............................A.....CG.................N... 1027 L L R L P K L I Y T N Q E T V K G S L E V A H F G A A P L Query_1 2053 TTACTGCGCTTGCCGAAG-TTGATTTATACCAACCAGGAAACGGTGAAA-GGAAGTCTGGAAGTGGCACATTTCGGAGCTGCTCCGCTGG 2140 0 1852 ..................-..............................-........................................ 1939 0 999 ........... 1009 1 1026 ..........-.......G.........C......CA.G..........A.........N.......N....-................. 939 E V T S T V W T L K T K E G K T I A S G T L A H Q P V G I Query_1 2141 AGGTGACTTCTACTGTCTGGACCCTGAAAAC--AAAAGAA-GGAAAGACAATTGCTTCGGGCACGCTGGCACACCAGCCGGTAGGTATCG 2227 0 1940 ...............................--.......-................................................. 2026 1 938 ...................N..........ACA.......A................................................. 849 2 869 ...N 872 G N C I P L G Q L E I P L D K V D V P S C L T L E A T L G D Query_1 2228 GCAATTGTATTCCGTTGGGGCAGCTGGAGATTCCATTGGATAAGGTGGACGTCCC-TTCATGTCTGACACTGGAAGCTACATTGGGAGAT 2316 0 2027 .......................................................-.................................. 2115 1 848 .......................................................C.................................. 759 2 873 A............ 885 Y A N S W H I W V Y P A A V Q K V A D E A Q L L M T D R L Query_1 2317 TACGCCAA-CAGCTGGCACATCTGGGTATATCCTGCTGCGGTACAGAAAGTAGCTGATGAAGCACAATTGCTGATGACCGACCGTCTGGA 2405 0 2116 ........-................................................................................. 2204 1 758 ........A................................................................................. 669 D A K A L Q R L Q E G G N V L L S L R K G S L P A E A G G E Query_1 2406 TGCAAAAGCTTTGCAACGTCTTCAGGAAGGTGGCAACGTACTGCTTTCTTTACGGAAAGGCTCCTTGCCTGCCGAAGCGGGAGGCGAAGT 2495 0 2205 .......................................................................................... 2294 1 668 .......................................................................................... 579 V V I G F S S I F W N T A W T L G Q A P H T L G I L C N P A Query_1 2496 AGTGATAGGTTTCTCTAGCATCTTCTGGAACACGGCCTGGACGCTGGGACAAGCACCGCACACACTGGGTATCCTGTGTAACCCCGCTCA 2585 0 2295 .......................................................................................... 2384 1 578 .......................................................................................... 489 H P A L S E F P T E Y Y S D Y Q W W D A M S H S G A I E V V Query_1 2586 TCCGGCACTTTCAGAGTTCCCTACAGAGTATTACAGTGATTATCAGTGGTGGGATGCCATGAGCCATTCCGGTGCCATCGAAGTGGTCAA 2675 0 2385 .......................................................................................... 2474 1 488 .......................................................................................... 399 K I D K N L Q P I V R V I D D W F T N R P L A L L F E V K V Query_1 2676 GATTGATAAAAACTTGCAGCCGATTGTACGAGTTATCGACGACTGGTTTACGAACCGTCCGCTGGCTTTGTTGTTCGAAGTGAAGGTGGG 2765 0 2475 .......................................................................................... 2564 1 398 .......................................................................................... 309 G K G K L L V S G I D F W Q D M D K R T E A R Q L L Y S L K Query_1 2766 TAAGGGTAAATTGCTTGTGTCAGGAATTGATTTCTGGCAGGATATGGACAAGCGTACGGAAGCCCGTCAGTTACTCTACAGCTTGAAGAA 2855 0 2565 .......................................................................................... 2654 1 308 .......................................................................................... 219 K Y M C G N R F N P S S E V D A K D L S I L F S I K N Q K * Query_1 2856 ATATATGTGCGGTAATCGCTTCAATCCCTCTTCTGAAGTCGATGCGAAAGATTTAAGTATTTTGTTTTCCATTAAAAATCAAAAATAACA 2945 0 2655 ..................................................................................... 2739 1 218 .......................................................................................... 129 H W K W I T D P N S S S V D K L A A A L E H H H H H H * D P Query_1 2946 TTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGC 3035 1 128 ..............................................NN.......................................... 39 A A N K A R K E Query_1 3036 TGCTAACAAAGCCCGAAAGGAA 3057 1 38 ..................A... 17 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 15579984 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 5,038 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200137.122 (CMR200137.19-931.P(931).E550A.pMCSG68)
QTDKIDLAGSWTFSTDSMDWSRVIELPGSMASNGFGEDIAVGTDWTGGIVDSSYFFKPSYAKYREAGNIKVPFWLQPVKYYKGKAWYQKEVVIPDSWEGKDISLFLERCHWESRLYIDGKEIGMQNALGAPHRYDLTGKLSAGKHVLMLCVDNRVKNIDPGENSHSISDHTQGNWNGVVGDMFLEVKPEVNVSSVKIMPERLAKKVSVSASLMNRYEKDANVVLEMTVGNEKVQQQCTLKPGENQVMMSLAMKGDIKCWDEFSPSLYDLKLSVKDADSGETDVYAERFGFRDVKVKDGKLTINDRRLFLRGTLDCAVFPKTGFPPTDVESWKKIYTTCRQHGLNHVRFHSWCPPEAAFAAADGMGMYLEIECSSWANQSTTIGDGGDLDRFIWEESERIVREFGNHPSFCMMMYGNEPAGEGSNAYLTNFVTTWKERDARRLYCSGAGWPNLPVNDFLSDSNPRIQAWGQGVKSIINAQAPRTDYDWSEYIGRFQQPMVSHEIGQWCVYPNFKEMAKYDGVMRPRNFEIFQETLAENGMAHLADSFLLASGKLQALCYKADIEAALRTKDFGGFQLLGLSDFPGQGTALVGVLDAFWEEKGYIRPEEYRRFCNSTVPLLRLPKLIYTNQETVKGSLEVAHFGAAPLEVTSTVWTLKTKEGKTIASGTLAHQPVGIGNCIPLGQLEIPLDKVDVPSCLTLEATLGDYANSWHIWVYPAAVQKVADEAQLLMTDRLDAKALQRLQEGGNVLLSLRKGSLPAEAGGEVVIGFSSIFWNTAWTLGQAPHTLGILCNPAHPALSEFPTEYYSDYQWWDAMSHSGAIEVVKIDKNLQPIVRVIDDWFTNRPLALLFEVKVGKGKLLVSGIDFWQDMDKRTEARQLLYSLKKYMCGNRFNPSSEVDAKDLSILFSIKNQK
CAGACTGATAAGATTGACCTGGCCGGCTCGTGGACATTTTCTACGGACAGCATGGACTGGAGCCGGGTGATTGAACTGCCGGGTTCAATGGCTTCCAATGGTTTTGGGGAAGATATTGCCGTGGGTACTGATTGGACGGGCGGTATTGTGGATTCTTCTTATTTCTTTAAACCTTCGTATGCCAAATACCGTGAGGCAGGAAATATCAAGGTACCTTTCTGGCTTCAGCCGGTAAAATATTACAAGGGTAAGGCGTGGTATCAGAAAGAGGTGGTGATTCCGGACAGTTGGGAAGGAAAGGACATTTCTCTCTTTTTGGAACGATGCCATTGGGAGAGCCGTTTGTATATAGACGGAAAGGAAATCGGCATGCAAAATGCTTTGGGGGCGCCCCATCGTTATGACCTGACAGGCAAGCTTTCAGCAGGGAAACATGTGTTGATGCTGTGTGTAGACAATCGGGTGAAAAACATTGATCCGGGGGAGAACTCACATAGTATTTCCGACCATACACAAGGAAACTGGAACGGGGTGGTAGGCGATATGTTCCTGGAAGTAAAGCCGGAAGTGAATGTGTCTTCCGTCAAGATTATGCCGGAGCGTCTGGCTAAGAAAGTCAGTGTGTCGGCTTCCTTGATGAACCGTTATGAAAAAGATGCCAATGTGGTACTGGAGATGACGGTAGGTAATGAAAAAGTACAGCAACAATGTACGTTGAAGCCGGGCGAAAATCAAGTGATGATGTCGCTGGCCATGAAGGGAGACATTAAGTGCTGGGATGAGTTTTCTCCATCCTTATATGATTTGAAGCTGAGTGTGAAGGATGCGGATAGCGGTGAAACGGATGTCTATGCGGAACGTTTTGGTTTCCGTGATGTGAAGGTGAAAGACGGCAAACTCACCATCAACGACCGCCGTTTGTTCCTGCGTGGTACGCTGGATTGTGCCGTATTTCCGAAGACCGGTTTCCCGCCCACGGATGTAGAATCCTGGAAAAAGATTTATACCACCTGTCGGCAGCACGGACTGAACCATGTGCGCTTCCATTCCTGGTGTCCGCCCGAAGCTGCTTTTGCAGCTGCCGATGGGATGGGTATGTACCTGGAGATAGAATGTTCTTCCTGGGCTAACCAGTCGACTACCATTGGCGATGGAGGCGATCTGGACCGCTTTATCTGGGAGGAAAGTGAACGCATCGTCCGTGAGTTTGGTAACCATCCTTCTTTCTGCATGATGATGTACGGTAACGAACCGGCTGGTGAGGGAAGTAATGCCTATCTGACTAATTTTGTTACTACCTGGAAAGAGCGCGATGCCCGCCGTTTATATTGTTCGGGTGCCGGATGGCCCAATTTGCCGGTTAACGACTTCTTGAGCGATTCCAATCCTCGTATTCAGGCGTGGGGACAAGGTGTGAAGAGTATTATCAACGCACAGGCTCCGCGTACCGACTATGACTGGTCAGAATACATCGGACGTTTCCAGCAGCCGATGGTGAGCCACGAAATCGGGCAGTGGTGTGTATATCCCAACTTCAAGGAAATGGCCAAATACGACGGGGTGATGCGCCCGCGTAATTTTGAGATATTCCAGGAAACACTGGCTGAAAACGGTATGGCACATTTGGCTGACAGCTTCCTGCTGGCTTCCGGAAAATTGCAGGCGTTGTGTTATAAGGCCGATATCGAAGCTGCTTTGCGTACAAAAGACTTCGGTGGATTCCAGTTACTGGGCTTGTCTGATTTCCCGGGGCAGGGTACGGCTTTGGTAGGAGTGCTCGATGCGTTCTGGGAAGAAAAAGGCTACATCCGTCCGGAAGAATACCGTCGTTTCTGTAATAGTACGGTACCATTACTGCGCTTGCCGAAGTTGATTTATACCAACCAGGAAACGGTGAAAGGAAGTCTGGAAGTGGCACATTTCGGAGCTGCTCCGCTGGAGGTGACTTCTACTGTCTGGACCCTGAAAACAAAAGAAGGAAAGACAATTGCTTCGGGCACGCTGGCACACCAGCCGGTAGGTATCGGCAATTGTATTCCGTTGGGGCAGCTGGAGATTCCATTGGATAAGGTGGACGTCCCTTCATGTCTGACACTGGAAGCTACATTGGGAGATTACGCCAACAGCTGGCACATCTGGGTATATCCTGCTGCGGTACAGAAAGTAGCTGATGAAGCACAATTGCTGATGACCGACCGTCTGGATGCAAAAGCTTTGCAACGTCTTCAGGAAGGTGGCAACGTACTGCTTTCTTTACGGAAAGGCTCCTTGCCTGCCGAAGCGGGAGGCGAAGTAGTGATAGGTTTCTCTAGCATCTTCTGGAACACGGCCTGGACGCTGGGACAAGCACCGCACACACTGGGTATCCTGTGTAACCCCGCTCATCCGGCACTTTCAGAGTTCCCTACAGAGTATTACAGTGATTATCAGTGGTGGGATGCCATGAGCCATTCCGGTGCCATCGAAGTGGTCAAGATTGATAAAAACTTGCAGCCGATTGTACGAGTTATCGACGACTGGTTTACGAACCGTCCGCTGGCTTTGTTGTTCGAAGTGAAGGTGGGTAAGGGTAAATTGCTTGTGTCAGGAATTGATTTCTGGCAGGATATGGACAAGCGTACGGAAGCCCGTCAGTTACTCTACAGCTTGAAGAAATATATGTGCGGTAATCGCTTCAATCCCTCTTCTGAAGTCGATGCGAAAGATTTAAGTATTTTGTTTTCCATTAAAAATCAAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,032 total letters Query= CMR200137.122 Length=3144 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4931 0.0 1_01C_C4_CMR200137.122_T7term.seq 1896 0.0 2_04C_CMR200137.122Seq171C04.seq 403 6e-115 Q T D K I D L A G S W T F S T D S M D W S R V I E L P G S M Query_1 202 CAGACTGATAAGATTGACCTGGCCGGCTCGTGGACATTTTCTACGGACAGCATGGACTGGAGCCGGGTGATTGAACTGCCGGGTTCAATG 291 0 1 .......................................................................................... 90 A S N G F G E D I A V G T D W T G G I V D S S Y F F K P S Y Query_1 292 GCTTCCAATGGTTTTGGGGAAGATATTGCCGTGGGTACTGATTGGACGGGCGGTATTGTGGATTCTTCTTATTTCTTTAAACCTTCGTAT 381 0 91 .......................................................................................... 180 A K Y R E A G N I K V P F W L Q P V K Y Y K G K A W Y Q K E Query_1 382 GCCAAATACCGTGAGGCAGGAAATATCAAGGTACCTTTCTGGCTTCAGCCGGTAAAATATTACAAGGGTAAGGCGTGGTATCAGAAAGAG 471 0 181 .......................................................................................... 270 V V I P D S W E G K D I S L F L E R C H W E S R L Y I D G K Query_1 472 GTGGTGATTCCGGACAGTTGGGAAGGAAAGGACATTTCTCTCTTTTTGGAACGATGCCATTGGGAGAGCCGTTTGTATATAGACGGAAAG 561 0 271 .......................................................................................... 360 E I G M Q N A L G A P H R Y D L T G K L S A G K H V L M L Query_1 562 GAAATCGGCATGCAAAATGCTTTGGGGGCGCCCCATCGTTATGACCTGACAGGCAAGCTTTCAG-CAGGGAAACATGTGTTGATGCTGTG 650 0 361 ................................................................-......................... 449 2 909 ...GN..N....A.N....T....-..C....A....C 873 C V D N R V K N I D P G E N S H S I S D H T Q G N W N G V V Query_1 651 TGTAGACAATCGGGTGAAAAACATTGATCCGGGGGAGAACTCACATAGTATTTCCGACCATACACAAGGAAACTGGAACGGGGTG-GTAG 739 0 450 .....................................................................................-.... 538 2 872 ..-..G.T..GN.AG..T....G.A.CAAT..TN.N......T....T...C..N.T...N..........T..CNTN.....G.A.... 784 G D M F L E V K P E V N V S S V K I M P E R L A K K V S V Query_1 740 GC-GATATGTTCCTGGAAGTAAAGCC-GGAAGTGAATGTGTCTTCCGTCAAGATTATGCCGGAGCGTCTGGCTAAGAAAGTCAGTGTGTC 827 0 539 ..-.......................-............................................................... 626 2 783 ..T....AA.-.N...NTTAC.....T.........N........G........A.AT.A..T...CAA.CNC..........CN..... 695 S A S L M N R Y E K D A N V V L E M T V G N E K V Q Q Q C T Query_1 828 GGCTTCCTTGATGAACCGTTATGAAAAAGA--TGCCAATGTGGTACTGGAGATGACGGTAGGTAATGAAAAAGTACAGCAACAATGTACG 915 0 627 ..............................--.......................................................... 714 2 694 .TTN.AGAN..G...GN.....TC......GA..T-.NCC..T.T...-..CN..N....T....GC..TC.AGC............... 607 L K P G E N Q V M M S L A M K G D I K C W D E F S P S L Y Query_1 916 TTGAAGCCGGGC-GAAAATCAAGTGATGATGTCGCTGGCCATGAAGGGAGACATTAAGTGCTGGGATGAGTTTTCTCCATCCTTATATGA 1004 0 715 ............-............................................................................. 803 2 606 .......AAAT.A.....A...C...N....GNATCC...GAA.T.....N......AA..A........A...N...... 526 D L K L S V K D A D S G E T D V Y A E R F G F R D V K V K D Query_1 1005 TTTGAAGCTGAGTGTGAAGGATGCGGATAGCGGTGAAACGGATGTCTATGCGGAACGTTTTGGTTTCCGTGATGTGAAGGTGAAAGACGG 1094 0 804 .......................................................................................... 893 2 441 ...T......ATT.. 427 G K L T I N D R R L F L R G T L D C A V F P K T G F P P T Query_1 1095 CAAACTCACCATCAACGACCGCCGTTTGTTCCTG--CGTGGTACGCTGGATTGTGC--CGTATTTCCGAAGACCGGTTTCCCGCCCACGG 1180 0 894 ..................................--....................--................................ 979 2 426 .............CC..T...TA-....GC.A.TTC....--.A.T...GA.A...ACN.C..........T.......T.NC..TNG.. 340 D V E S W K K I Y T T C R Q H G L N H V R F H S W C P P E A Query_1 1181 ATGTAGAATCCTGGAAAAAGATTTATACCACCTGTCGGCAGCACGGACTGAACCATGTGCGCTTCCATTCCTGGTGTCCGCCCGAAGCTG 1270 0 980 .......................................................................................... 1069 0 1686 ........ 1693 0 1872 ........... 1882 1 1004 ........... 994 2 339 .G..G.....N...G...-.....G.C.. 312 A F A A A D G M G M Y L E I E C S S W A N Q S T T I G D G G Query_1 1271 CTTTTGCAGCTGCCGATGGGATGGGTATGTACCTGGAGATAGAATGTTCTTCCTGGGCTAACCAGTCGACTACCATTGGCGATGGAGGCG 1360 0 1070 .......................................................................................... 1159 0 1694 .... 1697 2 2 ....-....... 12 D L D R F I W E E S E R I V R E F G N H P S F C M M M Y G N Query_1 1361 ATCTGGACCGCTTTATCTGGGAGGAAAGTGAACGCATCGTCCGTGAGTTTGGTAACCATCCTTCTTTCTGCATGATGATGTACGGTAACG 1450 0 1160 .......................................................................................... 1249 2 13 .......................................................................T.............G.... 102 E P A G E G S N A Y L T N F V T T W K E R D A R R L Y C S G Query_1 1451 AACCGGCTGGTGAGGGAAGTAATGCCTATCTGACTAATTTTGTTACTACCTGGAAAGAGCGCGATGCCCGCCGTTTATATTGTTCGGGTG 1540 0 1250 .......................................................................................... 1339 2 103 ..G..............................T.........A..A...G.N.....T.....C.......AA.GG.....NA.T.... 192 A G W P N L P V N D F L S D S N P R I Q A W G Q G V K S I I Query_1 1541 CCGGATGGCCCAATTTGCCGGTTAACGACTTCTTGAGCGATTCCAATCCTCGTATTCAGGCGTGGGGACAAGGTGTGAAGAGTATTATCA 1630 0 1340 .......................................................................................... 1429 2 193 ........A.......CTATC....G..........NTC.....N....CT.C....NC..C.TC......ACACC...A.TG....... 282 N A Q A P R T D Y D W S E Y I G R F Q Q P M V S H E I G Q W Query_1 1631 ACGCACAGGCTCCGCGTACCGACTATGACTGGTCAGAATACATCGGACGTTTCCAGCAGCCGATGGTGAGCCACGAAATCGGGCAGTGGT 1720 0 1430 .......................................................................................... 1519 2 283 ..NTG........C..G.TGC........G..A..-...CTT..CC.N.A.....C.TC..N.G..N..AAAC..G.C.TC..A.A..CN 371 C V Y P N F K E M A K Y D G V M R P R N F E I F Q A T L A Query_1 1721 GTGTATATCCCAACTTCAAGGAAATGGCCAAATAC-GACGGGG-TGATGCGCCCGCGTAATTTTGAGATATTCCAGGCTACACTGGCTGA 1808 0 1520 ...................................-.......-.................................AA........... 1607 2 372 ...C..............C................G.......A..G..A.TTT..-C......C.C..TC...TC..........N... 460 E N G M A H L A D S F L L A S G K L Q A L C Y K A D I E A A Query_1 1809 AAACGGTATGGCACATTTGGCTGACAGCTTCCTGCTGGCTTCCGGAAAATTGCAGG-CGTTGTGTTATA-AGGCCGATATCGAAGCTGCT 1896 0 1608 ........................................................-............-.................... 1695 0 1062 .......... 1071 1 1229 ........G........-...TNNNN...AN.N...NN.N.C 1189 2 461 NNT.C.N....AC.T....TT....NNT.........CT.C..A..C.......N.-TCN...... 525 L R T K D F G G F Q L L G L S D F P G Q G T A L V G V L Query_1 1897 TTGCGTACAAAAGACTTCGGTGG-ATTCCAGTTACTGGGCTTGTCTGATTTCCCGGGGC-AGGGTACGGCTTTG-GTA-GGAG-TGC-TC 1980 0 1696 .......................-...................................-..............-...-....-...-.. 1779 0 1072 .. 1073 1 1188 ...N.......NNNNN.......G...........NTNN..GN.NN..........A..N..N.....AN....A...A....N...C.. 1099 D A F W E E K G Y I R P E E Y R R F C N S T V P L L R L P Query_1 1981 GATGCGT-TCTGGGAAG-AAAAAGGCTACAT-CCGTCCGGAAGAATACCGTCGTTTCTGTAATAGTACGGTACCATTACTGCGCTTGCCG 2067 0 1780 .......-.........-.............-.......................................................... 1866 1 1098 .......N.........N.............T.......-.....NN......G...NN............................... 1010 K L I Y T N Q E T V K G S L E V A H F G A A P L E V T S T Query_1 2068 AAGTTGATTTATACCAACCAGG-AAACGGTGAAAGGAAGTCTGGAAGTGGCACATTTCGGAGCTGCT-CCGCTGGAGG-TGACTTCTACT 2154 0 1867 ......................-............................................-..........-........... 1953 0 999 ........... 1009 1 1009 ......................G............................................T....N.....G........... 920 V W T L K T K E G K T I A S G T L A H Q P V G I G N C I P Query_1 2155 GTCTGGACCCTGAAAACAAAAGAAGGAAAGACAATTGCTTCGGGCACGCTGGCACACCAGCC-GGTAGGTATCGGCAATTGTATTCCGTT 2243 0 1954 ..............................................................-........................... 2042 1 919 ...........................................C..................C........................... 830 L G Q L E I P L D K V D V P S C L T L E A T L G D Y A N S W Query_1 2244 GGGGCAGCTGGAGATTCCATTGGATAAGGTGGACGTCCCTTCATGTCTGACACTGGAAGCTACATTGGGAGATTACGCCAACAGCTGGCA 2333 0 2043 .......................................................................................... 2132 1 829 .........................................................................-................ 741 H I W V Y P A A V Q K V A D E A Q L L M T D R L D A K A L Q Query_1 2334 CATCTGGGTATATCCTGCTGCGGTACAGAAAGTAGCTGATGAAGCACAATTGCTGATGACCGACCGTCTGGATGCAAAAGCTTTGCAACG 2423 0 2133 .......................................................................................... 2222 1 740 .......................................................................................... 651 R L Q E G G N V L L S L R K G S L P A E A G G E V V I G F S Query_1 2424 TCTTCAGGAAGGTGGCAACGTACTGCTTTCTTTACGGAAAGGCTCCTTGCCTGCCGAAGCGGGAGGCGAAGTAGTGATAGGTTTCTCTAG 2513 0 2223 .......................................................................................... 2312 1 650 .......................................................................................... 561 S I F W N T A W T L G Q A P H T L G I L C N P A H P A L S E Query_1 2514 CATCTTCTGGAACACGGCCTGGACGCTGGGACAAGCACCGCACACACTGGGTATCCTGTGTAACCCCGCTCATCCGGCACTTTCAGAGTT 2603 0 2313 .......................................................................................... 2402 1 560 .......................................................................................... 471 F P T E Y Y S D Y Q W W D A M S H S G A I E V V K I D K N L Query_1 2604 CCCTACAGAGTATTACAGTGATTATCAGTGGTGGGATGCCATGAGCCATTCCGGTGCCATCGAAGTGGTCAAGATTGATAAAAACTTGCA 2693 0 2403 .......................................................................................... 2492 1 470 .......................................................................................... 381 Q P I V R V I D D W F T N R P L A L L F E V K V G K G K L L Query_1 2694 GCCGATTGTACGAGTTATCGACGACTGGTTTACGAACCGTCCGCTGGCTTTGTTGTTCGAAGTGAAGGTGGGTAAGGGTAAATTGCTTGT 2783 0 2493 .......................................................................................... 2582 1 380 .......................................................................................... 291 V S G I D F W Q D M D K R T E A R Q L L Y S L K K Y M C G N Query_1 2784 GTCAGGAATTGATTTCTGGCAGGATATGGACAAGCGTACGGAAGCCCGTCAGTTACTCTACAGCTTGAAGAAATATATGTGCGGTAATCG 2873 0 2583 .......................................................................................... 2672 1 290 .......................................................................................... 201 R F N P S S E V D A K D L S I L F S I K N Q K * H W K W I T Query_1 2874 CTTCAATCCCTCTTCTGAAGTCGATGCGAAAGATTTAAGTATTTTGTTTTCCATTAAAAATCAAAAATAACATTGGAAGTGGATAACGGA 2963 0 2673 ................................................................... 2739 1 200 .......................................................................................... 111 D P N S S S V D K L A A A L E H H H H H H * D P A A N K A R Query_1 2964 TCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAG-CCCGAA 3052 1 110 ...........................N.......................................................C...... 21 K E Query_1 3053 AGGAA 3057 1 20 .A... 16 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 15561228 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 5,032 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200137.123 (CMR200137.19-931.P(931).Y503F.pMCSG68)
QTDKIDLAGSWTFSTDSMDWSRVIELPGSMASNGFGEDIAVGTDWTGGIVDSSYFFKPSYAKYREAGNIKVPFWLQPVKYYKGKAWYQKEVVIPDSWEGKDISLFLERCHWESRLYIDGKEIGMQNALGAPHRYDLTGKLSAGKHVLMLCVDNRVKNIDPGENSHSISDHTQGNWNGVVGDMFLEVKPEVNVSSVKIMPERLAKKVSVSASLMNRYEKDANVVLEMTVGNEKVQQQCTLKPGENQVMMSLAMKGDIKCWDEFSPSLYDLKLSVKDADSGETDVYAERFGFRDVKVKDGKLTINDRRLFLRGTLDCAVFPKTGFPPTDVESWKKIYTTCRQHGLNHVRFHSWCPPEAAFAAADGMGMYLEIECSSWANQSTTIGDGGDLDRFIWEESERIVREFGNHPSFCMMMYGNEPAGEGSNAYLTNFVTTWKERDARRLYCSGAGWPNLPVNDFLSDSNPRIQAWGQGVKSIINAQAPRTDYDWSEYIGRFQQPMVSHEIGQWCVYPNFKEMAKYDGVMRPRNFEIFQETLAENGMAHLADSFLLASGKLQALCYKADIEAALRTKDFGGFQLLGLSDFPGQGTALVGVLDAFWEEKGYIRPEEYRRFCNSTVPLLRLPKLIYTNQETVKGSLEVAHFGAAPLEVTSTVWTLKTKEGKTIASGTLAHQPVGIGNCIPLGQLEIPLDKVDVPSCLTLEATLGDYANSWHIWVYPAAVQKVADEAQLLMTDRLDAKALQRLQEGGNVLLSLRKGSLPAEAGGEVVIGFSSIFWNTAWTLGQAPHTLGILCNPAHPALSEFPTEYYSDYQWWDAMSHSGAIEVVKIDKNLQPIVRVIDDWFTNRPLALLFEVKVGKGKLLVSGIDFWQDMDKRTEARQLLYSLKKYMCGNRFNPSSEVDAKDLSILFSIKNQK
CAGACTGATAAGATTGACCTGGCCGGCTCGTGGACATTTTCTACGGACAGCATGGACTGGAGCCGGGTGATTGAACTGCCGGGTTCAATGGCTTCCAATGGTTTTGGGGAAGATATTGCCGTGGGTACTGATTGGACGGGCGGTATTGTGGATTCTTCTTATTTCTTTAAACCTTCGTATGCCAAATACCGTGAGGCAGGAAATATCAAGGTACCTTTCTGGCTTCAGCCGGTAAAATATTACAAGGGTAAGGCGTGGTATCAGAAAGAGGTGGTGATTCCGGACAGTTGGGAAGGAAAGGACATTTCTCTCTTTTTGGAACGATGCCATTGGGAGAGCCGTTTGTATATAGACGGAAAGGAAATCGGCATGCAAAATGCTTTGGGGGCGCCCCATCGTTATGACCTGACAGGCAAGCTTTCAGCAGGGAAACATGTGTTGATGCTGTGTGTAGACAATCGGGTGAAAAACATTGATCCGGGGGAGAACTCACATAGTATTTCCGACCATACACAAGGAAACTGGAACGGGGTGGTAGGCGATATGTTCCTGGAAGTAAAGCCGGAAGTGAATGTGTCTTCCGTCAAGATTATGCCGGAGCGTCTGGCTAAGAAAGTCAGTGTGTCGGCTTCCTTGATGAACCGTTATGAAAAAGATGCCAATGTGGTACTGGAGATGACGGTAGGTAATGAAAAAGTACAGCAACAATGTACGTTGAAGCCGGGCGAAAATCAAGTGATGATGTCGCTGGCCATGAAGGGAGACATTAAGTGCTGGGATGAGTTTTCTCCATCCTTATATGATTTGAAGCTGAGTGTGAAGGATGCGGATAGCGGTGAAACGGATGTCTATGCGGAACGTTTTGGTTTCCGTGATGTGAAGGTGAAAGACGGCAAACTCACCATCAACGACCGCCGTTTGTTCCTGCGTGGTACGCTGGATTGTGCCGTATTTCCGAAGACCGGTTTCCCGCCCACGGATGTAGAATCCTGGAAAAAGATTTATACCACCTGTCGGCAGCACGGACTGAACCATGTGCGCTTCCATTCCTGGTGTCCGCCCGAAGCTGCTTTTGCAGCTGCCGATGGGATGGGTATGTACCTGGAGATAGAATGTTCTTCCTGGGCTAACCAGTCGACTACCATTGGCGATGGAGGCGATCTGGACCGCTTTATCTGGGAGGAAAGTGAACGCATCGTCCGTGAGTTTGGTAACCATCCTTCTTTCTGCATGATGATGTACGGTAACGAACCGGCTGGTGAGGGAAGTAATGCCTATCTGACTAATTTTGTTACTACCTGGAAAGAGCGCGATGCCCGCCGTTTATATTGTTCGGGTGCCGGATGGCCCAATTTGCCGGTTAACGACTTCTTGAGCGATTCCAATCCTCGTATTCAGGCGTGGGGACAAGGTGTGAAGAGTATTATCAACGCACAGGCTCCGCGTACCGACTATGACTGGTCAGAATACATCGGACGTTTCCAGCAGCCGATGGTGAGCCACGAAATCGGGCAGTGGTGTGTATATCCCAACTTCAAGGAAATGGCCAAATACGACGGGGTGATGCGCCCGCGTAATTTTGAGATATTCCAGGAAACACTGGCTGAAAACGGTATGGCACATTTGGCTGACAGCTTCCTGCTGGCTTCCGGAAAATTGCAGGCGTTGTGTTATAAGGCCGATATCGAAGCTGCTTTGCGTACAAAAGACTTCGGTGGATTCCAGTTACTGGGCTTGTCTGATTTCCCGGGGCAGGGTACGGCTTTGGTAGGAGTGCTCGATGCGTTCTGGGAAGAAAAAGGCTACATCCGTCCGGAAGAATACCGTCGTTTCTGTAATAGTACGGTACCATTACTGCGCTTGCCGAAGTTGATTTATACCAACCAGGAAACGGTGAAAGGAAGTCTGGAAGTGGCACATTTCGGAGCTGCTCCGCTGGAGGTGACTTCTACTGTCTGGACCCTGAAAACAAAAGAAGGAAAGACAATTGCTTCGGGCACGCTGGCACACCAGCCGGTAGGTATCGGCAATTGTATTCCGTTGGGGCAGCTGGAGATTCCATTGGATAAGGTGGACGTCCCTTCATGTCTGACACTGGAAGCTACATTGGGAGATTACGCCAACAGCTGGCACATCTGGGTATATCCTGCTGCGGTACAGAAAGTAGCTGATGAAGCACAATTGCTGATGACCGACCGTCTGGATGCAAAAGCTTTGCAACGTCTTCAGGAAGGTGGCAACGTACTGCTTTCTTTACGGAAAGGCTCCTTGCCTGCCGAAGCGGGAGGCGAAGTAGTGATAGGTTTCTCTAGCATCTTCTGGAACACGGCCTGGACGCTGGGACAAGCACCGCACACACTGGGTATCCTGTGTAACCCCGCTCATCCGGCACTTTCAGAGTTCCCTACAGAGTATTACAGTGATTATCAGTGGTGGGATGCCATGAGCCATTCCGGTGCCATCGAAGTGGTCAAGATTGATAAAAACTTGCAGCCGATTGTACGAGTTATCGACGACTGGTTTACGAACCGTCCGCTGGCTTTGTTGTTCGAAGTGAAGGTGGGTAAGGGTAAATTGCTTGTGTCAGGAATTGATTTCTGGCAGGATATGGACAAGCGTACGGAAGCCCGTCAGTTACTCTACAGCTTGAAGAAATATATGTGCGGTAATCGCTTCAATCCCTCTTCTGAAGTCGATGCGAAAGATTTAAGTATTTTGTTTTCCATTAAAAATCAAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,159 total letters Query= CMR200137.123 Length=3144 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4935 0.0 2_06F_D4_CMR200137.123_T7term.seq 1397 0.0 Q T D K I D L A G S W T F S T D S M D W S R V I E L P G S M Query_1 202 CAGACTGATAAGATTGACCTGGCCGGCTCGTGGACATTTTCTACGGACAGCATGGACTGGAGCCGGGTGATTGAACTGCCGGGTTCAATG 291 0 1 .......................................................................................... 90 A S N G F G E D I A V G T D W T G G I V D S S Y F F K P S Y Query_1 292 GCTTCCAATGGTTTTGGGGAAGATATTGCCGTGGGTACTGATTGGACGGGCGGTATTGTGGATTCTTCTTATTTCTTTAAACCTTCGTAT 381 0 91 .......................................................................................... 180 A K Y R E A G N I K V P F W L Q P V K Y Y K G K A W Y Q K E Query_1 382 GCCAAATACCGTGAGGCAGGAAATATCAAGGTACCTTTCTGGCTTCAGCCGGTAAAATATTACAAGGGTAAGGCGTGGTATCAGAAAGAG 471 0 181 .......................................................................................... 270 V V I P D S W E G K D I S L F L E R C H W E S R L Y I D G K Query_1 472 GTGGTGATTCCGGACAGTTGGGAAGGAAAGGACATTTCTCTCTTTTTGGAACGATGCCATTGGGAGAGCCGTTTGTATATAGACGGAAAG 561 0 271 .......................................................................................... 360 2 901 ............A.. 887 E I G M Q N A L G A P H R Y D L T G K L S A G K H V L M L C Query_1 562 GAAATCGGCATGCAAAATGCTTTGGGGGCGCCCCATCGTTATGACCTGACAGGCAAGCTTTCAGCAGGGAAACATGTGTTGATGCTGTGT 651 0 361 .......................................................................................... 450 V D N R V K N I D P G E N S H S I S D H T Q G N W N G V V G Query_1 652 GTAGACAATCGGGTGAAAAACATTGATCCGGGGGAGAACTCACATAGTATTTCCGACCATACACAAGGAAACTGGAACGGGGTGGTAGGC 741 0 451 .......................................................................................... 540 D M F L E V K P E V N V S S V K I M P E R L A K K V S V S A Query_1 742 GATATGTTCCTGGAAGTAAAGCCGGAAGTGAATGTGTCTTCCGTCAAGATTATGCCGGAGCGTCTGGCTAAGAAAGTCAGTGTGTCGGCT 831 0 541 .......................................................................................... 630 S L M N R Y E K D A N V V L E M T V G N E K V Q Q Q C T L K Query_1 832 TCCTTGATGAACCGTTATGAAAAAGATGCCAATGTGGTACTGGAGATGACGGTAGGTAATGAAAAAGTACAGCAACAATGTACGTTGAAG 921 0 631 .......................................................................................... 720 P G E N Q V M M S L A M K G D I K C W D E F S P S L Y D L K Query_1 922 CCGGGCGAAAATCAAGTGATGATGTCGCTGGCCATGAAGGGAGACATTAAGTGCTGGGATGAGTTTTCTCCATCCTTATATGATTTGAAG 1011 0 721 .......................................................................................... 810 L S V K D A D S G E T D V Y A E R F G F R D V K V K D G K L Query_1 1012 CTGAGTGTGAAGGATGCGGATAGCGGTGAAACGGATGTCTATGCGGAACGTTTTGGTTTCCGTGATGTGAAGGTGAAAGACGGCAAACTC 1101 0 811 .......................................................................................... 900 T I N D R R L F L R G T L D C A V F P K T G F P P T D V E S Query_1 1102 ACCATCAACGACCGCCGTTTGTTCCTGCGTGGTACGCTGGATTGTGCCGTATTTCCGAAGACCGGTTTCCCGCCCACGGATGTAGAATCC 1191 0 901 .......................................................................................... 990 W K K I Y T T C R Q H G L N H V R F H S W C P P E A A F A A Query_1 1192 TGGAAAAAGATTTATACCACCTGTCGGCAGCACGGACTGAACCATGTGCGCTTCCATTCCTGGTGTCCGCCCGAAGCTGCTTTTGCAGCT 1281 0 991 .......................................................................................... 1080 0 1686 ............ 1697 0 1872 ........... 1882 A D G M G M Y L E I E C S S W A N Q S T T I G D G G D L D R Query_1 1282 GCCGATGGGATGGGTATGTACCTGGAGATAGAATGTTCTTCCTGGGCTAACCAGTCGACTACCATTGGCGATGGAGGCGATCTGGACCGC 1371 0 1081 .......................................................................................... 1170 F I W E E S E R I V R E F G N H P S F C M M M Y G N E P A G Query_1 1372 TTTATCTGGGAGGAAAGTGAACGCATCGTCCGTGAGTTTGGTAACCATCCTTCTTTCTGCATGATGATGTACGGTAACGAACCGGCTGGT 1461 0 1171 .......................................................................................... 1260 E G S N A Y L T N F V T T W K E R D A R R L Y C S G A G W P Query_1 1462 GAGGGAAGTAATGCCTATCTGACTAATTTTGTTACTACCTGGAAAGAGCGCGATGCCCGCCGTTTATATTGTTCGGGTGCCGGATGGCCC 1551 0 1261 .......................................................................................... 1350 N L P V N D F L S D S N P R I Q A W G Q G V K S I I N A Q A Query_1 1552 AATTTGCCGGTTAACGACTTCTTGAGCGATTCCAATCCTCGTATTCAGGCGTGGGGACAAGGTGTGAAGAGTATTATCAACGCACAGGCT 1641 0 1351 .......................................................................................... 1440 P R T D F D W S E Y I G R F Q Q P M V S H E I G Q W C V Y P Query_1 1642 CCGCGTACCGACTTTGACTGGTCAGAATACATCGGACGTTTCCAGCAGCCGATGGTGAGCCACGAAATCGGGCAGTGGTGTGTATATCCC 1731 0 1441 .............A............................................................................ 1530 N F K E M A K Y D G V M R P R N F E I F Q E T L A E N G M A Query_1 1732 AACTTCAAGGAAATGGCCAAATACGACGGGGTGATGCGCCCGCGTAATTTTGAGATATTCCAGGAAACACTGGCTGAAAACGGTATGGCA 1821 0 1531 .......................................................................................... 1620 H L A D S F L L A S G K L Q A L C Y K A D I E A A L R T K D Query_1 1822 CATTTGGCTGACAGCTTCCTGCTGGCTTCCGGAAAATTGCAGGCGTTGTGTTATAAGGCCGATATCGAAGCTGCTTTGCGTACAAAAGAC 1911 0 1621 .......................................................................................... 1710 0 1062 ............ 1073 F G G F Q L L G L S D F P G Q G T A L V G V L D A F W E E K Query_1 1912 TTCGGTGGATTCCAGTTACTGGGCTTGTCTGATTTCCCGGGGCAGGGTACGGCTTTGGTAGGAGTGCTCGATGCGTTCTGGGAAGAAAAA 2001 0 1711 .......................................................................................... 1800 G Y I R P E E Y R R F C N S T V P L L R L P K L I Y T N Q E Query_1 2002 GGCTACATCCGTCCGGAAGAATACCGTCGTTTCTGTAATAGTACGGTACCATTACTGCGCTTGCCGAAGTTGATTTATACCAACCAGGAA 2091 0 1801 .......................................................................................... 1890 0 999 ........... 1009 T V K G S L E V A H F G A A P L E V T S T V W T L K T K Query_1 2092 ACGGTGAAAGGAAGTCTGGAAGTGGCACATTTCGGAGCTGCTCCGCTGGAGGTGACTTCTACTGTCTGGACCC-TG-AAAACAAAAG--A 2177 0 1891 .........................................................................-..-..........--. 1976 2 924 .......C..N.........NGA. 901 E G K T I A S G T L A H Q P V G I G N C I P L G Q L E Query_1 2178 AGGAAAG-ACAATTGCTT-CGGG-CACGCTGGCACA-CCAG-CCGG-TAGGTAT-CGGCAATTGT-ATTCCGTTGGGGCAGCTGGAGA-T 2258 0 1977 .......-..........-....-............-....-....-.......-..........-......................-. 2057 2 900 .......G......NA..T....A..N.........N....A....N..N..T.T......N.N.TN....N.G............NNG. 811 I P L D K V D V P S C L T L E A T L G D Y A N S W H I W Query_1 2259 TCC-ATTGGATAAGG-TGGACGTCCC--TTCA-TGTCTGA-CACTGGAAGCTACATT-GGGAGATTACGCCAACAGCTGGCACATCTGGG 2341 0 2058 ...-...........-..........--....-.......-................-................................ 2140 2 810 ...N..G......N.N..........CT....N.N.....N........N.......T................................ 721 V Y P A A V Q K V A D E A Q L L M T D R L D A K A L Q R L Query_1 2342 TATATCCTGCTGCGGTACA-GAAAGTAGCTGATGAA-GCACAATTGCTGATGACCGACCGTCTGGATGCAAAA-GCTTTGCAACGTCTTC 2428 0 2141 ...................-................-....................................-................ 2227 2 720 ...N..T............N................A....................................A................ 631 Q E G G N V L L S L R K G S L P A E A G G E V V I G F S S I Query_1 2429 AGGAAGGTGGCAACGTACTGCTTTCTTTACGGAAAGGCTCCTTGCCTGCCGAAGCGGGAGGCGAAGTAGTGATAGGTTTCTCTAGCATCT 2518 0 2228 .......................................................................................... 2317 2 630 .......N.................................................................................. 541 F W N T A W T L G Q A P H T L G I L C N P A H P A L S E F P Query_1 2519 TCTGGAACACGGCCTGGACGCTGGGACAAGCACCGCACACACTGGGTATCCTGTGTAACCCCGCTCATCCGGCACTTTCAGAGTTCCCTA 2608 0 2318 .......................................................................................... 2407 2 540 .......................................................................................... 451 T E Y Y S D Y Q W W D A M S H S G A I E V V K I D K N L Q P Query_1 2609 CAGAGTATTACAGTGATTATCAGTGGTGGGATGCCATGAGCCATTCCGGTGCCATCGAAGTGGTCAAGATTGATAAAAACTTGCAGCCGA 2698 0 2408 .......................................................................................... 2497 2 450 .......................................................................................... 361 I V R V I D D W F T N R P L A L L F E V K V G K G K L L V S Query_1 2699 TTGTACGAGTTATCGACGACTGGTTTACGAACCGTCCGCTGGCTTTGTTGTTCGAAGTGAAGGTGGGTAAGGGTAAATTGCTTGTGTCAG 2788 0 2498 .......................................................................................... 2587 2 360 .......................................................................................... 271 G I D F W Q D M D K R T E A R Q L L Y S L K K Y M C G N R F Query_1 2789 GAATTGATTTCTGGCAGGATATGGACAAGCGTACGGAAGCCCGTCAGTTACTCTACAGCTTGAAGAAATATATGTGCGGTAATCGCTTCA 2878 0 2588 .......................................................................................... 2677 2 270 .......................................................................................... 181 N P S S E V D A K D L S I L F S I K N Q K * H W K W I T D P Query_1 2879 ATCCCTCTTCTGAAGTCGATGCGAAAGATTTAAGTATTTTGTTTTCCATTAAAAATCAAAAATAACATTGGAAGTGGATAACGGATCCGA 2968 0 2678 .............................................................. 2739 2 180 .......................................................................................... 91 N S S S V D K L A A A L E H H H H H H * D P A A N K A R K Query_1 2969 ATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAG 3054 2 90 .......................N................................................T............. 5 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12845716 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,159 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CMR200148.116 (CMR200148.28-761.P(761).E513A.pMCSG68)
KDAEMDRFISDLMGRMTLQEKLGQLNLPAGNDLVSGAVKNSKMAEAIRAGEVGGFFNVKGVDKIYQMQRMAVEETRLGIPLIVGADVIHGYETIFPIPLALSCSWDTAAVTRMARISATEASADGISWTFSPMVDICRDARWGRIAEGSGEDPYLGALMAGAYVRGYQGDGMKQNNEIMACVKHFALYGASESGRDYNSVDMSRNLMYNVYLAPYKGAVEAGVGSVMSSFNTINGVPATADKWLLTDLLRNEWGFTGFVVTDYNSIGEMKTHGVADLKEASARALNAGTDMDMVAHGFLHTLEASLKEKAVTQERIDEACRRVLEAKYKLGLFENPYKYCDTLRGRKELFTEANRKAAREIAAETFVLLKNEGKLLPLQKKGRIALIGPMADAQNNMCGTWNMDCQTDRHVTMYEAFRRAVGDKATVSYAKGSNVYYSEHIEKGAVEPRPLTRGDDRQLRAEALRVAASADVIVAALGESAEMSGESSSRTDIQIPDAQKDLLKALIATGKPVVLALFTGRPLDLCWESEHVPAILNVWFAGSEAGDAIADVMFGEVSPSGKLTTSFPRAVGQLPLYYNHLNTGRPDTDDTTFNRYGSNYIDQSNEPLYPFGYGLSYTTFRYGNLQLSAERMAKGGQLKVTVPVTNSGECDGVEIVQLYLHDVYAEISRPVKELKAFRRVALKKGETQNVEFVLDEDDLKYYNSRLEYGYEPGEFEVMVGPDSRNVQHATFVAE
AAGGATGCGGAGATGGACCGCTTTATCAGTGACCTGATGGGAAGGATGACCTTGCAGGAAAAGTTAGGACAGTTGAATCTGCCGGCTGGGAATGACCTGGTGTCGGGAGCAGTGAAGAACAGCAAGATGGCAGAAGCTATCCGAGCTGGTGAGGTCGGCGGCTTTTTCAATGTGAAGGGAGTGGATAAGATTTACCAGATGCAGCGTATGGCGGTGGAGGAAACTCGTCTGGGAATTCCTTTGATAGTGGGTGCCGATGTGATTCACGGGTACGAAACAATCTTCCCGATTCCGTTGGCCCTGTCTTGTAGCTGGGATACGGCGGCGGTGACACGTATGGCACGTATTTCTGCCACGGAAGCCAGTGCCGATGGAATCAGCTGGACCTTCAGTCCGATGGTAGACATCTGTCGGGATGCCCGCTGGGGACGTATTGCAGAAGGAAGTGGAGAGGACCCGTACCTCGGGGCGTTGATGGCTGGAGCCTATGTGCGCGGTTATCAGGGTGACGGCATGAAGCAGAACAATGAAATCATGGCCTGTGTGAAGCACTTTGCGCTGTATGGAGCTTCGGAATCGGGACGTGACTACAATTCGGTGGATATGAGTCGAAACCTGATGTATAATGTGTACCTGGCTCCTTATAAAGGGGCGGTGGAAGCCGGAGTGGGTTCGGTGATGAGCTCGTTCAATACCATCAACGGGGTACCTGCTACAGCTGACAAATGGCTGCTGACGGATTTGCTCCGCAATGAGTGGGGGTTCACGGGGTTTGTGGTGACCGACTACAATTCGATTGGTGAGATGAAGACTCATGGGGTGGCCGACTTGAAGGAGGCTTCTGCACGGGCGTTGAATGCAGGAACGGACATGGATATGGTGGCACATGGTTTCTTGCATACGCTGGAAGCTTCATTGAAGGAGAAGGCCGTGACGCAGGAGCGGATTGACGAGGCTTGTCGTCGGGTATTGGAAGCCAAGTATAAGTTAGGATTGTTTGAAAATCCTTATAAGTATTGTGATACGCTTCGGGGACGCAAGGAATTGTTTACGGAGGCGAATCGTAAAGCGGCACGTGAGATTGCGGCTGAAACGTTTGTGCTGTTGAAGAACGAGGGTAAGTTGTTGCCTTTGCAGAAAAAAGGACGCATTGCATTGATTGGGCCGATGGCTGATGCGCAGAACAATATGTGCGGCACGTGGAACATGGATTGTCAGACAGACCGTCATGTGACGATGTACGAAGCTTTCCGTCGTGCGGTAGGTGATAAGGCTACGGTTTCTTATGCCAAGGGAAGTAATGTGTATTATAGTGAGCATATTGAGAAAGGGGCGGTCGAACCTCGTCCGCTGACACGTGGCGATGACCGTCAGTTGCGGGCTGAGGCTTTGCGCGTGGCGGCTTCTGCCGATGTGATTGTGGCCGCATTAGGTGAGAGTGCTGAGATGAGCGGAGAGTCTTCTTCTCGTACAGATATTCAGATTCCGGATGCGCAGAAAGATTTGTTGAAGGCATTGATAGCTACCGGAAAGCCGGTGGTACTGGCTTTGTTTACCGGTCGTCCGCTGGATTTATGCTGGGAGTCTGAGCATGTTCCGGCTATCCTGAACGTGTGGTTTGCCGGCAGTGAAGCGGGTGATGCCATTGCCGATGTGATGTTTGGAGAAGTATCTCCTTCGGGTAAGCTGACTACGAGTTTCCCACGTGCGGTGGGACAGTTGCCGCTTTATTATAATCACCTGAATACGGGTCGTCCGGATACGGATGACACTACTTTCAATCGTTATGGCAGCAATTACATCGACCAGAGTAATGAACCGCTTTATCCTTTTGGCTATGGTTTGAGTTATACCACTTTCCGTTACGGTAATTTGCAGTTGAGTGCGGAGCGTATGGCCAAGGGTGGGCAGTTGAAGGTAACCGTGCCTGTAACCAATTCCGGCGAGTGTGACGGAGTAGAGATTGTGCAGTTGTATCTTCACGATGTGTATGCAGAAATCTCCCGTCCGGTGAAGGAGCTGAAAGCTTTCCGCCGTGTGGCCCTTAAAAAGGGAGAGACACAGAATGTAGAGTTTGTACTCGATGAGGATGATTTGAAGTATTATAATTCTCGTCTGGAATATGGATATGAACCGGGAGAGTTTGAAGTGATGGTGGGTCCGGACAGCCGGAATGTGCAGCACGCGACTTTTGTGGCTGAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,401 total letters Query= CMR200148.116 Length=2607 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3963 0.0 1_01E_E4_CMR200148.116_T7term.seq 1820 0.0 2_04E_CMR200148.116Seq171E04.seq 848 0.0 K D A E M D R F I S D L M G R M T L Q E K L G Q L N L P A G Query_1 202 AAGGATGCGGAGATGGACCGCTTTATCAGTGACCTGATGGGAAGGATGACCTTGCAGGAAAAGTTAGGACAGTTGAATCTGCCGGCTGGG 291 0 1 .......................................................................................... 90 0 1814 ...A........... 1828 1 523 ...A........... 509 N D L V S G A V K N S K M A E A I R A G E V G G F F N V K G Query_1 292 AATGACCTGGTGTCGGGAGCAGTGAAGAACAGCAAGATGGCAGAAGCTATCCGAGCTGGTGAGGTCGGCGGCTTTTTCAATGTGAAGGGA 381 0 91 .......................................................................................... 180 V D K I Y Q M Q R M A V E E T R L G I P L I V G A D V I H G Query_1 382 GTGGATAAGATTTACCAGATGCAGCGTATGGCGGTGGAGGAAACTCGTCTGGGAATTCCTTTGATAGTGGGTGCCGATGTGATTCACGGG 471 0 181 .......................................................................................... 270 0 1407 ............. 1419 0 1647 ............ 1658 1 690 ............ 679 1 929 ........... 919 2 142 .............. 129 Y E T I F P I P L A L S C S W D T A A V T R M A R I S A T E Query_1 472 TACGAAACAATCTTCCCGATTCCGTTGGCCCTGTCTTGTAGCTGGGATACGGCGGCGGTGACACGTATGGCACGTATTTCTGCCACGGAA 561 0 271 .......................................................................................... 360 0 582 ........... 572 A S A D G I S W T F S P M V D I C R D A R W G R I A E G S G Query_1 562 GCCAGTGCCGATGGAATCAGCTGGACCTTCAGTCCGATGGTAGACATCTGTCGGGATGCCCGCTGGGGACGTATTGCAGAAGGAAGTGGA 651 0 361 .......................................................................................... 450 E D P Y L G A L M A G A Y V R G Y Q G D G M K Q N N E I M A Query_1 652 GAGGACCCGTACCTCGGGGCGTTGATGGCTGGAGCCTATGTGCGCGGTTATCAGGGTGACGGCATGAAGCAGAACAATGAAATCATGGCC 741 0 451 .......................................................................................... 540 C V K H F A L Y G A S E S G R D Y N S V D M S R N L M Y N V Query_1 742 TGTGTGAAGCACTTTGCGCTGTATGGAGCTTCGGAATCGGGACGTGACTACAATTCGGTGGATATGAGTCGAAACCTGATGTATAATGTG 831 0 541 .......................................................................................... 630 0 784 ............ 795 0 294 ........... 284 Y L A P Y K G A V E A G V G S V M S S F N T I N G V P A T Query_1 832 TACCTGGCTCCTTATAAAGGGGCGGTGGAAGCCGGAGTGGGTTCGGTGATGAGCTC-GTTCAATACCATCAACGGGGTACCTGCTACAGC 920 0 631 ........................................................-................................. 719 0 1327 ...........C... 1341 2 887 ....N...N...NTA.AC.......A....C..C......T....-.N.T...A....T 830 2 220 ...........C... 206 A D K W L L T D L L R N E W G F T G F V V T D Y N S I G E M Query_1 921 TGACA-AATGGCTGCTGACGGATTTGCTCCGCAATGAGTGGGGGTTCACGGGGTTTGTGGTGACCGACTACAATTCGATTGGTGAGATGA 1009 0 720 .....-.................................................................................... 808 0 586 ............ 597 2 829 ..C..C..A.T...N.....C..C....AT....A..NN...ANNA....A...NNN.NC..T.AT.TN....C..T............. 740 K T H G V A D L K E A S A R A L N A G T D M D M V A H G F L Query_1 1010 AGACTCATGGGGTGGCCGACTTGAAGGAGGCTTCTGCACGGGCGTTGAATGCAGGAACGGACATGGATATGGTGGCACATGGTTTCTTGC 1099 0 809 .......................................................................................... 898 2 739 ....A..CAC.TC....A..N.C..CAC..N..AA....C.....GC............T...C.......AN.............N... 650 H T L E A S L K E K A V T Q E R I D E A C R R V L E A K Y Query_1 1100 ATACGCT-GGAAGCTTCATTGAAGGAGAAGGCCGTGACGCAGGAGCGGATTGACGAGGCTTGTCGTCG-GGTATTGGAAGCCAAGTATAA 1187 0 899 .......-............................................................-..................... 986 2 649 ..C.CT.T.....A......C...A........N...................A............ATC....N................ 560 K L G L F E N P Y K Y C D T L R G R K E L F T E A N R K A A Query_1 1188 GTTAGGATTGTTTGAAAATCCTTATAAGTATTGTGATACGCTTCGGGGACGCAAGGAATTGTTTACGGAGGCGAATCGTAAAGCGGCACG 1277 0 987 .......................................................................................... 1076 2 559 .......N....CAC...............NCA-...CA.G....N.........C.........N.....T............C..... 471 R E I A A E T F V L L K N E G K L L P L Q K K G R I A L I G Query_1 1278 TGAGATTGCGGCTGAAACGTTTGTGCTGTTGAAGAACGAGGGTAAGTTGTTGCCTTTGCAGAAAAAAGGACGCATTGCATTGATTGGGCC 1367 0 1077 .......................................................................................... 1166 1 1232 ..............NN.....N....CANTNN...CN. 1195 2 470 ...C.A..................AAG.CA.C....N..............N...C.C........CA......A............... 381 P M A D A Q N N M C G T W N M D C Q T D R H V T M Y E Query_1 1368 GATGGCTGATGC-GCAGAAC--AATA-TGTGCGGCACGTGGAACA-TGGA-TTGTC-AGACAGACCGTCA-TGTGACGA-TGTACGAAGC 1448 0 1167 ............-.......--....-..................-....-.....-.............-........-.......... 1247 0 1489 .....-......A--G..T-... 1507 1 1194 ....NN..NNNNN....GN.NC....N.NNN..NNNN.N.A....A....A.....N.....NG......A...NNN..A........AG 1105 1 848 .....-......A--G..T-... 830 2 380 .....N...C..-.......--C...-........T.........-....-.....-......T.N....-.....A..-...C...... 300 A F R R A V G D K A T V S Y A K G S N V Y Y S E H I E Query_1 1449 TTTCCGTC-GTGCGGT-AGGTGATAAGG-CTACGG--TTTCTTATGCC-AAGGGAA-GTAATG-TGT-ATTATA-GTGAGCATATTGAGA 1528 0 1248 ........-.......-...........-......--...........-.......-......-...-......-............... 1327 0 646 . 646 1 1104 C.......C.......N...........G....C.GT........N..C.......A......N...T......A........NN..... 1015 2 299 .......G-..NT.C.-...........-......--...NC...N..-.......-..C.N.-.C.-N.....-............... 220 K G A V E P R P L T R G D D R Q L R A E A L R V A A S A D Query_1 1529 AA-GGGGCGG-TCGAA-CCTCGTCCGCTGACACGTGGCGATGACCGTCAGTTGCGGGCTGAGGCTTTGCGCGTGGCGGCTTCTGCC-GAT 1614 0 1328 ..-.......-.....-.....................................................................-... 1413 0 647 ..-.......-.G... 660 0 252 ....-... 258 0 1647 ....-... 1653 1 1014 ..A.......G.....A.........N..............................N.................T..........C... 925 1 690 ....-... 684 2 219 ..-.......-.....-....A..............................T....N.........AA......T..-AAG....-... 135 V I V A A L G E S A E M S G A S S S R T D I Q I P D A Q K Query_1 1615 GTGATTGTGGCCGCATTAGGTGAGAGTGCTGAGATGAGCGGAGCTT-CTTCTTCTCGTACAGATATTCAGATT-CCGGATGCGCAGAAAG 1702 0 1414 ...........................................AG.-..........................-................ 1501 0 1174 ...........CA 1186 0 259 ...... 264 0 1654 ..... 1658 1 924 ..............................................T..........................-................ 836 1 683 ..... 679 2 134 .......A.T....-.C...........A..............-AC-A.......T.A...............GGA.....A........ 48 D L L K A L I A T G K P V V L A L F T G R P L D L C W E S E Query_1 1703 ATTTGTTGAAGGCATTGATAGCTACCGGAAAGCCGGTGGTACTGGCTTTGTTTACCGGTCGTCCGCTGGATTTATGCTGGGAGTCTGAGC 1792 0 1502 .......................................................................................... 1591 0 1187 ..A... 1192 1 835 .......................................................................................... 746 2 47 .-.CC.........-.....CAGG.................. 8 H V P A I L N V W F A G S E A G D A I A D V M F G E V S P S Query_1 1793 ATGTTCCGGCTATCCTGAACGTGTGGTTTGCCGGCAGTGAAGCGGGTGATGCCATTGCCGATGTGATGTTTGGAGAAGTATCTCCTTCGG 1882 0 1592 .......................................................................................... 1681 0 252 ............ 263 0 1407 ............ 1418 1 745 .......................................................................................... 656 2 141 ............ 130 G K L T T S F P R A V G Q L P L Y Y N H L N T G R P D T D D Query_1 1883 GTAAGCTGACTACGAGTTTCCCACGTGCGGTGGGACAGTTGCCGCTTTATTATAATCACCTGAATACGGGTCGTCCGGATACGGATGACA 1972 0 1682 .......................................................................................... 1771 1 655 .......................................................................................... 566 T T F N R Y G S N Y I D Q S N E P L Y P F G Y G L S Y T T F Query_1 1973 CTACTTTCAATCGTTATGGCAGCAATTACATCGACCAGAGTAATGAACCGCTTTATCCTTTTGGCTATGGTTTGAGTTATACCACTTTCC 2062 0 1772 .......................................................................................... 1861 0 13 ...G........... 27 1 565 .......................................................................................... 476 R Y G N L Q L S A E R M A K G G Q L K V T V P V T N S G E C Query_1 2063 GTTACGGTAATTTGCAGTTGAGTGCGGAGCGTATGGCCAAGGGTGGGCAGTTGAAGGTAACCGTGCCTGTAACCAATTCCGGCGAGTGTG 2152 0 1862 .......................................................................................... 1951 1 475 .......................................................................................... 386 D G V E I V Q L Y L H D V Y A E I S R P V K E L K A F R R V Query_1 2153 ACGGAGTAGAGATTGTGCAGTTGTATCTTCACGATGTGTATGCAGAAATCTCCCGTCCGGTGAAGGAGCTGAAAGCTTTCCGCCGTGTGG 2242 0 1952 .......................................................................................... 2041 1 385 .......................................................................................... 296 A L K K G E T Q N V E F V L D E D D L K Y Y N S R L E Y G Y Query_1 2243 CCCTTAAAAAGGGAGAGACACAGAATGTAGAGTTTGTACTCGATGAGGATGATTTGAAGTATTATAATTCTCGTCTGGAATATGGATATG 2332 0 2042 .......................................................................................... 2131 1 295 .......................................................................................... 206 E P G E F E V M V G P D S R N V Q H A T F V A E * H W K W I Query_1 2333 AACCGGGAGAGTTTGAAGTGATGGTGGGTCCGGACAGCCGGAATGTGCAGCACGCGACTTTTGTGGCTGAATAACATTGGAAGTGGATAA 2422 0 2132 ....................................................................... 2202 1 205 .......................................................................................... 116 T D P N S S S V D K L A A A L E H H H H H H * D P A A N K A Query_1 2423 CGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCC 2512 1 115 ...............................N.N........................................................ 26 R K E Query_1 2513 --GAAAGGAA 2520 1 25 CGA....... 16 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 11266500 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 24, 2017 11:00 AM Number of letters in database: 4,401 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2