Clone ID: IDP00423.105 (IDP00423.25-962.P(962)..pMCSG73)
AIPAWQPLAETIYKSEHDLRKYQAIKLSNGMTVLLVSDTQAPKSLAALALPVGSLEDPDNQLGLAHYLEHMVLMGSKHFPEPGSFSEFLKKHGGSHNASTASYRTAFYLEIENDALAPAVERLADAIAEPLLDPINADRERNAVNAELTMARSRDGMRMAQVNAETLNPAHPSARFSGGNLETLRDKPDGKLHDELVSFYHRYYSANLMVGVLYSNQSLEQLAQLAADTFGRIPNRDAKVPTITVPVVTPDQTGIIIHYVPAQPRKQIKVDFRIANNSADFRSKTDTYISYLISNRSKNTLSDWLQKQGLADAINAGADPMLDRNGGVFSITVSLTDKGLAQRDVVVAAIFDYINMLHKEGIKKSYFDEIAHVLNLDFRYPSITRDMDYIEWLVDMMLRVPVAHTLDAPYLADQYDPKAIASRLAEMTPENARIWFVSPEEPHNKVAYFVDAPYQVDKIGVQRMKEWQQLGQKIALSLPALNPYIPDNFTLIKADKNITRPQNVADQPGLRVFYMPSQYFADEPKADITVAFRNPHALNSARHQVLFALTDYLAGLSLDQLSYQASIGGISFSTAPNNGLYVNAGGFTQRMPQLLTSLVSGYASFTPTEEQLVQAKSWYREQLDVAEKGKAYELAIQPAKLLSNVPYSERSERRKLLDSISVQDVLTYRDDLLKQSAIEVLAVGNMTAEQVTELTESLKKQLNLIGTTWWVGEDVIIEKTQLANMERVGSSSDAALAAVYVPTGYTEIAGMARSALLGQIIQPWFYDQLRTEEQLGYAVFSFPMSVGHQWGIGFLLQSNSKEPNYLYQRYLAFYPQAEKRLREMKPDDFEQYKQGLVNQLLQRPQTLDEEAERYRKDFNLNNFAFDSREKMIAQVKQLTANELADFFQQAVIKPQGLALLSQVKGQGQAGGFAVPEGWTTYPTTSALQATLPQKVLAP
GCAATTCCTGCGTGGCAACCTTTGGCGGAAACCATTTACAAAAGTGAACATGACTTACGTAAATATCAGGCAATAAAATTATCCAATGGTATGACGGTACTACTGGTTTCCGATACACAAGCCCCTAAATCTTTGGCGGCCTTAGCGCTACCGGTAGGGTCACTGGAAGACCCAGATAATCAGCTCGGCTTGGCCCACTACTTGGAGCATATGGTGCTCATGGGGTCCAAACATTTCCCTGAACCTGGCAGTTTTTCCGAATTCCTAAAAAAGCATGGGGGGAGCCATAATGCGAGCACGGCTTCTTACCGAACCGCTTTCTATCTTGAAATTGAAAATGATGCATTGGCACCTGCGGTTGAGCGGTTGGCGGATGCCATTGCTGAACCCTTGTTAGACCCCATTAATGCGGATCGTGAGCGTAATGCTGTCAATGCTGAATTGACGATGGCCCGTTCACGCGATGGTATGCGTATGGCGCAAGTTAATGCGGAAACGCTTAATCCGGCACATCCAAGCGCACGTTTTTCTGGTGGTAACCTTGAAACTCTTAGGGACAAGCCCGATGGCAAGCTACATGATGAATTGGTGAGCTTCTATCACCGCTACTATTCTGCCAATCTCATGGTCGGGGTGTTGTACAGCAACCAATCATTAGAGCAGCTAGCACAATTAGCCGCAGACACCTTCGGCCGTATCCCTAATAGGGATGCAAAAGTACCCACGATTACGGTCCCAGTCGTGACACCTGATCAAACGGGAATCATCATTCACTATGTTCCTGCTCAACCTCGTAAACAGATAAAAGTCGATTTCCGTATTGCGAACAATAGTGCGGATTTTCGTAGTAAAACAGATACCTATATTAGCTACTTGATCAGTAATCGCAGTAAAAACACCTTATCTGACTGGCTACAAAAACAGGGGCTGGCAGATGCAATCAATGCGGGTGCTGACCCAATGTTGGATAGAAATGGGGGGGTGTTCTCTATCACTGTCTCACTAACCGATAAGGGGCTGGCACAACGTGATGTTGTCGTCGCGGCAATTTTTGATTACATCAATATGCTGCATAAAGAGGGGATTAAAAAAAGCTATTTCGATGAAATTGCACATGTATTGAATCTCGATTTCCGTTATCCCTCTATCACCCGTGATATGGACTATATCGAATGGCTCGTGGATATGATGTTACGAGTTCCTGTTGCACATACGCTTGATGCGCCTTATCTGGCTGATCAGTATGATCCCAAAGCAATTGCATCCCGATTGGCCGAGATGACGCCTGAAAATGCCCGCATCTGGTTTGTCAGCCCTGAAGAACCGCATAACAAAGTAGCTTATTTTGTCGATGCACCTTATCAGGTCGATAAAATAGGCGTACAGCGGATGAAAGAATGGCAGCAACTGGGGCAAAAGATTGCGCTAAGTTTGCCAGCACTGAACCCGTACATTCCTGATAATTTCACTCTGATCAAAGCAGACAAGAACATTACCCGCCCACAGAACGTGGCAGACCAGCCAGGATTGCGGGTGTTTTACATGCCAAGTCAGTATTTTGCTGACGAACCCAAAGCCGACATTACGGTTGCTTTCCGCAACCCTCATGCATTGAACTCAGCTCGCCATCAGGTGCTTTTTGCCCTGACGGATTACCTTGCGGGTCTCTCACTTGATCAACTGAGTTATCAGGCCTCTATTGGTGGGATCAGTTTCTCTACCGCACCGAACAATGGCTTGTATGTTAATGCTGGTGGTTTTACACAGCGTATGCCGCAGTTACTGACATCTTTGGTTTCAGGCTATGCCAGTTTTACTCCGACAGAAGAGCAATTGGTACAGGCTAAATCTTGGTATCGTGAGCAATTAGACGTGGCAGAGAAAGGCAAAGCTTATGAGTTGGCTATTCAGCCCGCTAAGCTGCTATCGAATGTGCCTTATTCGGAGCGAAGTGAGCGACGTAAACTACTTGATAGCATCAGTGTGCAGGATGTGCTGACCTATAGAGATGATTTACTGAAACAGTCTGCGATAGAAGTTTTGGCCGTGGGCAATATGACAGCCGAACAAGTCACTGAACTCACTGAATCGCTGAAAAAACAGCTGAACTTAATCGGAACCACGTGGTGGGTCGGTGAAGATGTCATTATTGAGAAAACACAGTTGGCCAATATGGAACGGGTCGGCAGTAGTTCTGACGCGGCTTTGGCAGCAGTTTATGTCCCTACTGGCTATACAGAAATTGCTGGTATGGCACGTAGTGCCTTGTTAGGGCAGATCATTCAACCATGGTTCTATGATCAATTACGGACAGAAGAACAGCTTGGTTATGCTGTATTTTCTTTCCCAATGTCGGTTGGTCATCAATGGGGCATCGGCTTCTTACTGCAAAGTAATAGTAAGGAACCTAATTATCTTTACCAGCGATACCTCGCATTTTATCCGCAAGCTGAAAAACGCCTGCGCGAAATGAAGCCCGATGATTTTGAACAATATAAGCAGGGGCTGGTCAATCAACTATTGCAAAGGCCACAGACATTAGATGAAGAGGCAGAGCGCTACCGTAAAGACTTCAATCTCAATAATTTTGCATTTGATAGCCGTGAGAAGATGATTGCTCAAGTGAAACAGCTTACGGCTAATGAACTGGCGGATTTCTTCCAGCAAGCGGTCATTAAACCACAAGGTCTGGCACTGCTTTCTCAAGTTAAAGGTCAGGGGCAGGCCGGAGGATTCGCAGTACCGGAGGGATGGACGACTTATCCAACCACTTCCGCTTTACAGGCTACGCTGCCGCAGAAGGTATTGGCACCA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,948 total letters Query= IDP00423.105 Length=3219 Score E Sequences producing significant alignments: (Bits) Value 0_wt 5075 0.0 2_12F_F6_IDP00423.105_T7term.seq 1900 0.0 1_06F_F6_IDP00423.105_v73F.seq 1519 0.0 W S H P Q F E K G T E N L Y F Q S N A A I P A W Q P L A E T Query_1 145 TGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGCAATTCCTGCGTGGCAACCTTTGGCGGAAACC 234 0 1 ................................. 33 1 46 ...........A........G....------........................................................... 129 I Y K S E H D L R K Y Q A I K L S N G M T V L L V S D T Q A Query_1 235 ATTTACAAAAGTGAACATGACTTACGTAAATATCAGGCAATAAAATTATCCAATGGTATGACGGTACTACTGGTTTCCGATACACAAGCC 324 0 34 .......................................................................................... 123 1 130 .......................................................................................... 219 P K S L A A L A L P V G S L E D P D N Q L G L A H Y L E H M Query_1 325 CCTAAATCTTTGGCGGCCTTAGCGCTACCGGTAGGGTCACTGGAAGACCCAGATAATCAGCTCGGCTTGGCCCACTACTTGGAGCATATG 414 0 124 .......................................................................................... 213 1 220 .......................................................................................... 309 V L M G S K H F P E P G S F S E F L K K H G G S H N A S T A Query_1 415 GTGCTCATGGGGTCCAAACATTTCCCTGAACCTGGCAGTTTTTCCGAATTCCTAAAAAAGCATGGGGGGAGCCATAATGCGAGCACGGCT 504 0 214 .......................................................................................... 303 0 2376 ..... 2380 2 504 ..... 500 1 310 .......................................................................................... 399 S Y R T A F Y L E I E N D A L A P A V E R L A D A I A E P L Query_1 505 TCTTACCGAACCGCTTTCTATCTTGAAATTGAAAATGATGCATTGGCACCTGCGGTTGAGCGGTTGGCGGATGCCATTGCTGAACCCTTG 594 0 304 .......................................................................................... 393 0 2381 ...... 2386 2 499 ...... 494 1 400 .......................................................................................... 489 L D P I N A D R E R N A V N A E L T M A R S R D G M R M A Q Query_1 595 TTAGACCCCATTAATGCGGATCGTGAGCGTAATGCTGTCAATGCTGAATTGACGATGGCCCGTTCACGCGATGGTATGCGTATGGCGCAA 684 0 394 .......................................................................................... 483 1 490 .......................................................................................... 579 V N A E T L N P A H P S A R F S G G N L E T L R D K P D G K Query_1 685 GTTAATGCGGAAACGCTTAATCCGGCACATCCAAGCGCACGTTTTTCTGGTGGTAACCTTGAAACTCTTAGGGACAAGCCCGATGGCAAG 774 0 484 .......................................................................................... 573 1 580 ..........T............................................................................... 669 L H D E L V S F Y H R Y Y S A N L M V G V L Y S N Q S L E Q Query_1 775 CTACATGATGAATTGGTGAGCTTCTATCACCGCTACTATTCTGCCAATCTCATGGTCGGGGTGTTGTACAGCAACCAATCATTAGAGCAG 864 0 574 .......................................................................................... 663 1 670 .......................................................................................... 759 L A Q L A A D T F G R I P N R D A K V P T I T V P V V T P D Query_1 865 CTAGCACAATTAGCCGCAGACACCTTCGGCCGTATCCCTAATAGGGATGCAAAAGTACCCACGATTACGGTCCCAGTCGTGACACCTGAT 954 0 664 .......................................................................................... 753 1 760 .......................................................................................... 849 Q T G I I I H Y V P A Q P R K Q I K V D F R I A N N S A D Query_1 955 CAAACGGGAATCATCATTCACTATGTT-CCTGCT-CAACCTCGTAAACAGATAAAAGTCGA-TTTCCGTATTGCGAACAATAGTGCGGAT 1041 0 754 ...........................-......-..........................-............................ 840 0 1127 ....-....... 1137 1 850 ...........................C......NN.........................T........ 919 F R S K T D T Y I S Y L I S N R S K N T L S D W L Q K Q G L Query_1 1042 TTTCGTAGTAAAACAGATACCTATATTAGCTACTTGATCAGTAATCGCAGTAAAAACACCTTATCTGACTGGCTACAAAAACAGGGGCTG 1131 0 841 .......................................................................................... 930 0 1013 ........ 1020 A D A I N A G A D P M L D R N G G V F S I T V S L T D K G L Query_1 1132 GCAGATGCAATCAATGCGGGTGCTGACCCAATGTTGGATAGAAATgggggggTGTTCTCTATCACTGTCTCACTAACCGATAAGGGGCTG 1221 0 931 .......................................................................................... 1020 0 1021 ... 1023 A Q R D V V V A A I F D Y I N M L H K E G I K K S Y F D E I Query_1 1222 GCACAACGTGATGTTGTCGTCGCGGCAATTTTTGATTACATCAATATGCTGCATAAAGAGGGGATTaaaaaaaGCTATTTCGATGAAATT 1311 0 1021 .......................................................................................... 1110 A H V L N L D F R Y P S I T R D M D Y I E W L V D M M L R V Query_1 1312 GCACATGTATTGAATCTCGATTTCCGTTATCCCTCTATCACCCGTGATATGGACTATATCGAATGGCTCGTGGATATGATGTTACGAGTT 1401 0 1111 .......................................................................................... 1200 0 809 ........... 819 P V A H T L D A P Y L A D Q Y D P K A I A S R L A E M T P E Query_1 1402 CCTGTTGCACATACGCTTGATGCGCCTTATCTGGCTGATCAGTATGATCCCAAAGCAATTGCATCCCGATTGGCCGAGATGACGCCTGAA 1491 0 1201 .......................................................................................... 1290 N A R I W F V S P E E P H N K V A Y F V D A P Y Q V D K I G Query_1 1492 AATGCCCGCATCTGGTTTGTCAGCCCTGAAGAACCGCATAACAAAGTAGCTTATTTTGTCGATGCACCTTATCAGGTCGATAAAATAGGC 1581 0 1291 .......................................................................................... 1380 V Q R M K E W Q Q L G Q K I A L S L P A L N P Y I P D N F T Query_1 1582 GTACAGCGGATGAAAGAATGGCAGCAACTGGGGCAAAAGATTGCGCTAAGTTTGCCAGCACTGAACCCGTACATTCCTGATAATTTCACT 1671 0 1381 .......................................................................................... 1470 L I K A D K N I T R P Q N V A D Q P G L R V F Y M P S Q Y F Query_1 1672 CTGATCAAAGCAGACAAGAACATTACCCGCCCACAGAACGTGGCAGACCAGCCAGGATTGCGGGTGTTTTACATGCCAAGTCAGTATTTT 1761 0 1471 .......................................................................................... 1560 A D E P K A D I T V A F R N P H A L N S A R H Q V L F A L T Query_1 1762 GCTGACGAACCCAAAGCCGACATTACGGTTGCTTTCCGCAACCCTCATGCATTGAACTCAGCTCGCCATCAGGTGCTTTTTGCCCTGACG 1851 0 1561 .......................................................................................... 1650 D Y L A G L S L D Q L S Y Q A S I G G I S F S T A P N N G L Query_1 1852 GATTACCTTGCGGGTCTCTCACTTGATCAACTGAGTTATCAGGCCTCTATTGGTGGGATCAGTTTCTCTACCGCACCGAACAATGGCTTG 1941 0 1651 .......................................................................................... 1740 2 1143 ..... 1139 Y V N A G G F T Q R M P Q L L T S L V S G Y A S F T P T E Query_1 1942 TATGTTAATGCTGGTGGTTTTACACAGCGT-ATGCCGCAGTTACTGA-CATCTTTGGTTTCAGGCTATGCCAGTTTTACTCCGACAGAAG 2029 0 1741 ..............................-................-.......................................... 1828 2 1138 ................N..AC...-.....T................A...........-...NN...NN-.....A............. 1052 E Q L V Q A K S W Y R E Q L D V A E K G K A Y E L A I Q P A Query_1 2030 AGCAATTGGTACAGGCTAAATCTTGGTAT-CGTGAGCAATTAGACGTGGCAGAGAAAGGCAAAGCTTATGAGTTGGCTATTCAGCCCGCT 2118 0 1829 .............................-............................................................ 1917 2 1051 ....-........................T............................................................ 963 K L L S N V P Y S E R S E R R K L L D S I S V Q D V L T Y R Query_1 2119 AAGCTGCTATCGAATGTGCCTTATTCGGAGCGAAGTGAGCGACGTAAACTACTTGATAGCATCAGTGTGCAGGATGTGCTGACCTATAGA 2208 0 1918 .......................................................................................... 2007 2 962 .......................................................................................... 873 D D L L K Q S A I E V L A V G N M T A E Q V T E L T E S L K Query_1 2209 GATGATTTACTGAAACAGTCTGCGATAGAAGTTTTGGCCGTGGGCAATATGACAGCCGAACAAGTCACTGAACTCACTGAATCGCTGAAA 2298 0 2008 .......................................................................................... 2097 2 872 .......................................................................................... 783 K Q L N L I G T T W W V G E D V I I E K T Q L A N M E R V G Query_1 2299 AAACAGCTGAACTTAATCGGAACCACGTGGTGGGTCGGTGAAGATGTCATTATTGAGAAAACACAGTTGGCCAATATGGAACGGGTCGGC 2388 0 2098 .......................................................................................... 2187 2 782 .......................................................................................... 693 S S S D A A L A A V Y V P T G Y T E I A G M A R S A L L G Q Query_1 2389 AGTAGTTCTGACGCGGCTTTGGCAGCAGTTTATGTCCCTACTGGCTATACAGAAATTGCTGGTATGGCACGTAGTGCCTTGTTAGGGCAG 2478 0 2188 .......................................................................................... 2277 2 692 .......................................................................................... 603 I I Q P W F Y D Q L R T E E Q L G Y A V F S F P M S V G H Q Query_1 2479 ATCATTCAACCATGGTTCTATGATCAATTACGGACAGAAGAACAGCTTGGTTATGCTGTATTTTCTTTCCCAATGTCGGTTGGTCATCAA 2568 0 2278 .......................................................................................... 2367 2 602 .......................................................................................... 513 W G I G F L L Q S N S K E P N Y L Y Q R Y L A F Y P Q A E K Query_1 2569 TGGGGCATCGGCTTCTTACTGCAAAGTAATAGTAAGGAACCTAATTATCTTTACCAGCGATACCTCGCATTTTATCCGCAAGCTGAAAAA 2658 0 2368 .......................................................................................... 2457 2 512 .......................................................................................... 423 R L R E M K P D D F E Q Y K Q G L V N Q L L Q R P Q T L D E Query_1 2659 CGCCTGCGCGAAATGAAGCCCGATGATTTTGAACAATATAAGCAGGGGCTGGTCAATCAACTATTGCAAAGGCCACAGACATTAGATGAA 2748 0 2458 .......................................................................................... 2547 2 422 .......................................................................................... 333 E A E R Y R K D F N L N N F A F D S R E K M I A Q V K Q L T Query_1 2749 GAGGCAGAGCGCTACCGTAAAGACTTCAATCTCAATAATTTTGCATTTGATAGCCGTGAGAAGATGATTGCTCAAGTGAAACAGCTTACG 2838 0 2548 .......................................................................................... 2637 2 332 .......................................................................................... 243 A N E L A D F F Q Q A V I K P Q G L A L L S Q V K G Q G Q A Query_1 2839 GCTAATGAACTGGCGGATTTCTTCCAGCAAGCGGTCATTAAACCACAAGGTCTGGCACTGCTTTCTCAAGTTAAAGGTCAGGGGCAGGCC 2928 0 2638 .......................................................................................... 2727 2 242 .......................................................................................... 153 G G F A V P E G W T T Y P T T S A L Q A T L P Q K V L A P * Query_1 2929 GGAGGATTCGCAGTACCGGAGGGATGGACGACTTATCCAACCACTTCCGCTTTACAGGCTACGCTGCCGCAGAAGGTATTGGCACCATAA 3018 0 2728 ....................................................................................... 2814 2 152 .......................................................................................... 63 H W K W I T D P N S S S V D K L A A A L E H H H H H H * D P Query_1 3019 CATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCG 3108 2 62 .................--..... 41 2 45 ..... 41 A A N K A R Query_1 3109 GCTGCTAACAAAG-CCCGAAA 3128 2 40 .............C....... 20 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 15665694 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 4,948 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP00510.102 (IDP00510.20-207.P(207)..pMCSG68)
VDQSNPYRLMDEAAKKTFTRLKNEQPKIKQNPDYLRTIVREELLPFVQIKYAGALVLGSYYKDATPAQREAYFNAFGKYLEQAYGQALALYHGQTYDVAPDQPLGDANIVAIRVTILDPSGRPPVRLDFQWRKNSQTGNWQAYDMIAEGVSMISTKQNEWASILRQKGVDGLTQQLLSAAKQPITLDK
GTCGATCAAAGCAACCCTTATCGTCTGATGGATGAGGCAGCGAAGAAAACCTTTACGCGTCTGAAAAATGAGCAGCCTAAGATTAAGCAAAATCCAGATTATCTCCGCACCATTGTACGCGAAGAGCTGTTGCCGTTTGTTCAGATCAAATACGCCGGCGCGTTAGTACTGGGTAGCTATTATAAAGATGCTACACCTGCACAGCGTGAAGCGTATTTCAATGCATTCGGTAAATATCTGGAGCAGGCATACGGGCAGGCATTGGCGTTGTATCACGGCCAAACTTACGATGTGGCACCAGACCAGCCTTTAGGGGATGCCAACATCGTTGCTATCCGCGTCACCATTCTCGATCCAAGTGGGCGTCCACCTGTCCGGTTAGATTTCCAATGGCGTAAAAATAGCCAAACCGGTAACTGGCAGGCTTATGACATGATCGCCGAAGGGGTGAGCATGATTAGCACCAAACAGAATGAATGGGCCTCTATCCTGCGCCAAAAGGGTGTGGATGGTTTGACCCAACAATTGCTGAGTGCGGCTAAACAGCCAATCACCTTAGATAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 574 total letters Query= IDP00510.102 Length=969 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1018 0.0 V D Q S N P Y R L M D E A A K K T F T R L K N E Q P K I K Q Query_1 202 GTCGATCAAAGCAACCCTTATCGTCTGATGGATGAGGCAGCGAAGAAAACCTTTACGCGTCTGAAAAATGAGCAGCCTAAGATTAAGCAA 291 0 1 .......................................................................................... 90 N P D Y L R T I V R E E L L P F V Q I K Y A G A L V L G S Y Query_1 292 AATCCAGATTATCTCCGCACCATTGTACGCGAAGAGCTGTTGCCGTTTGTTCAGATCAAATACGCCGGCGCGTTAGTACTGGGTAGCTAT 381 0 91 .......................................................................................... 180 Y K D A T P A Q R E A Y F N A F G K Y L E Q A Y G Q A L A L Query_1 382 TATAAAGATGCTACACCTGCACAGCGTGAAGCGTATTTCAATGCATTCGGTAAATATCTGGAGCAGGCATACGGGCAGGCATTGGCGTTG 471 0 181 .......................................................................................... 270 Y H G Q T Y D V A P D Q P L G D A N I V A I R V T I L D P S Query_1 472 TATCACGGCCAAACTTACGATGTGGCACCAGACCAGCCTTTAGGGGATGCCAACATCGTTGCTATCCGCGTCACCATTCTCGATCCAAGT 561 0 271 .......................................................................................... 360 G R P P V R L D F Q W R K N S Q T G N W Q A Y D M I A E G V Query_1 562 GGGCGTCCACCTGTCCGGTTAGATTTCCAATGGCGTAAAAATAGCCAAACCGGTAACTGGCAGGCTTATGACATGATCGCCGAAGGGGTG 651 0 361 .......................................................................................... 450 S M I S T K Q N E W A S I L R Q K G V D G L T Q Q L L S A A Query_1 652 AGCATGATTAGCACCAAACAGAATGAATGGGCCTCTATCCTGCGCCAAAAGGGTGTGGATGGTTTGACCCAACAATTGCTGAGTGCGGCT 741 0 451 .......................................................................................... 540 K Q P I T L D K Query_1 742 AAACAGCCAATCACCTTAGATAAA 765 0 541 ........................ 564 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 511460 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 574 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP00563.103 (IDP00563.29-676.P(676)..pMCSG73)
ADTTTTQTSSKKHSTDTMVVTATGNERSSFEAPMMVTVIEGNAPTSQTAATAADMLRQVPGLTVTGSGRTNGQDVVMRGYGKQGVLTLVDGVRQGTDTGHLNSTFLDPALVKRIEIVRGPAALLYGSGALGGVIAYETVDAADMLQPGQNSGYRVYSSAATGDHSFGLGASAFGRTDDLDGILSFGTRDIGNIRQSNGFNAPNDETISNVLAKGTWQIDSIQSLSANLRYYNNSAIEPKNPQTSAPSSTNVMTNRSTIQRDAQLRYNIKPLDQEWLNATAQVYYSEVEINARPQGSAEEGREQTTEGVKLENRTRLFIESPASHLLTYGTETYKQEQTPGGATESFPQAKIRFSSGWLQDEITLRDLPVSILAGTRYDNYSGSSDGYADVDADKWSSRGAISITPTDWLMLFGSYAQAFRAPTMGEMYNDSKHFAIPIRPGLTLTNYWVPNPNLKPETNETQEYGFGLRFSDLLMAEDDLQFKVSYFDTKAKDYISTRVDMQAMTTTSVNIDQAKIWGWDASMSYKTALFNWDLAYNRTRGKNQNTDEWLDTINPDTVTSIVDVPVANSGFSVGWIGTFANRSSRVSSSTPQAGYGVNDFYVSYKGQEAFKGMTTTMLLGNVFEKEYYTPQGIPQDGRNVKFFVSYQW
GCTGATACCACAACCACTCAAACCAGCAGTAAAAAGCACAGCACCGACACCATGGTCGTCACAGCAACCGGTAATGAGCGTAGCAGTTTTGAAGCGCCAATGATGGTAACGGTCATCGAAGGGAATGCCCCTACCAGCCAAACCGCCGCGACGGCCGCAGATATGCTGCGACAAGTACCAGGGCTGACCGTCACTGGCAGCGGGCGTACCAACGGTCAAGATGTGGTGATGCGAGGTTACGGTAAACAAGGCGTGTTGACACTGGTTGATGGTGTTCGTCAGGGGACCGATACCGGTCACTTGAACTCCACTTTCCTCGATCCTGCGTTAGTCAAACGTATCGAAATCGTCCGTGGGCCAGCAGCACTGCTGTACGGCAGTGGCGCTCTCGGCGGGGTTATTGCCTATGAAACCGTGGATGCAGCGGACATGCTGCAACCGGGCCAAAACAGTGGTTATCGGGTTTACAGCTCTGCCGCAACGGGTGATCACAGCTTCGGTCTGGGAGCCAGCGCCTTTGGCCGCACCGATGATCTGGATGGTATCCTCTCTTTCGGTACCCGTGATATCGGCAACATTCGTCAAAGCAACGGTTTTAATGCCCCGAATGATGAAACTATCAGTAATGTATTGGCAAAAGGCACTTGGCAGATAGACTCGATTCAATCGTTAAGCGCCAATCTGCGTTATTACAACAACAGTGCAATAGAGCCAAAGAATCCGCAAACCAGCGCACCATCCAGTACCAATGTCATGACCAATCGTTCAACGATCCAGCGTGATGCCCAGCTCAGATATAATATTAAGCCTCTCGATCAAGAATGGTTGAATGCCACGGCACAGGTTTATTACTCCGAAGTGGAAATCAATGCCCGTCCACAAGGTTCAGCCGAAGAAGGCCGCGAGCAGACAACCGAAGGTGTGAAGCTGGAAAACCGTACCCGTCTGTTTATCGAAAGCCCAGCCTCCCACTTGCTGACCTATGGTACTGAAACCTATAAACAGGAACAGACCCCCGGTGGGGCAACGGAAAGTTTCCCACAGGCCAAAATTCGGTTTAGTTCGGGCTGGCTACAAGATGAAATCACCTTACGCGACCTGCCTGTCTCCATATTAGCCGGTACGCGTTATGACAATTACAGTGGTAGCAGCGATGGCTATGCCGATGTCGATGCCGATAAATGGTCATCACGTGGTGCAATCAGCATTACACCAACTGATTGGCTGATGCTGTTTGGCTCCTATGCTCAGGCTTTCCGCGCCCCAACCATGGGTGAGATGTATAACGATTCCAAACACTTTGCGATCCCTATCCGGCCTGGGTTGACACTCACCAACTATTGGGTCCCCAACCCAAATCTGAAACCAGAAACCAATGAGACCCAGGAATACGGTTTTGGCCTACGTTTCAGTGATTTACTGATGGCTGAAGATGACCTGCAATTTAAAGTCAGTTACTTCGATACCAAGGCTAAAGACTACATCAGTACCCGCGTTGACATGCAAGCCATGACCACCACATCGGTGAATATCGATCAGGCAAAAATCTGGGGTTGGGATGCCAGCATGAGCTATAAAACGGCCTTGTTTAACTGGGATCTGGCCTATAACCGCACCCGTGGTAAAAATCAGAATACCGATGAATGGCTCGACACCATCAACCCGGATACCGTCACCAGTATCGTTGATGTACCTGTCGCCAACAGTGGTTTCTCCGTCGGTTGGATTGGGACCTTCGCTAATCGCTCCAGCCGCGTTTCCAGTTCAACACCGCAAGCCGGTTATGGCGTCAATGATTTCTATGTGAGCTATAAAGGCCAGGAAGCATTCAAAGGCATGACCACAACAATGTTATTGGGTAATGTCTTCGAAAAAGAGTATTACACGCCACAAGGCATCCCACAAGATGGCCGTAACGTGAAATTTTTCGTGAGTTATCAGTGG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,143 total letters Query= IDP00563.103 Length=2349 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3507 0.0 A D T T T T Q T S S K K H S T D T M V V T A T G N E R S S F Query_1 202 GCTGATACCACAACCACTCAAACCAGCAGTAAAAAGCACAGCACCGACACCATGGTCGTCACAGCAACCGGTAATGAGCGTAGCAGTTTT 291 0 1 .......................................................................................... 90 E A P M M V T V I E G N A P T S Q T A A T A A D M L R Q V P Query_1 292 GAAGCGCCAATGATGGTAACGGTCATCGAAGGGAATGCCCCTACCAGCCAAACCGCCGCGACGGCCGCAGATATGCTGCGACAAGTACCA 381 0 91 .......................................................................................... 180 G L T V T G S G R T N G Q D V V M R G Y G K Q G V L T L V D Query_1 382 GGGCTGACCGTCACTGGCAGCGGGCGTACCAACGGTCAAGATGTGGTGATGCGAGGTTACGGTAAACAAGGCGTGTTGACACTGGTTGAT 471 0 181 .......................................................................................... 270 0 1663 ....... 1657 G V R Q G T D T G H L N S T F L D P A L V K R I E I V R G P Query_1 472 GGTGTTCGTCAGGGGACCGATACCGGTCACTTGAACTCCACTTTCCTCGATCCTGCGTTAGTCAAACGTATCGAAATCGTCCGTGGGCCA 561 0 271 .......................................................................................... 360 0 1656 ..... 1652 A A L L Y G S G A L G G V I A Y E T V D A A D M L Q P G Q N Query_1 562 GCAGCACTGCTGTACGGCAGTGGCGCTCTCGGCGGGGTTATTGCCTATGAAACCGTGGATGCAGCGGACATGCTGCAACCGGGCCAAAAC 651 0 361 .......................................................................................... 450 S G Y R V Y S S A A T G D H S F G L G A S A F G R T D D L D Query_1 652 AGTGGTTATCGGGTTTACAGCTCTGCCGCAACGGGTGATCACAGCTTCGGTCTGGGAGCCAGCGCCTTTGGCCGCACCGATGATCTGGAT 741 0 451 .......................................................................................... 540 G I L S F G T R D I G N I R Q S N G F N A P N D E T I S N V Query_1 742 GGTATCCTCTCTTTCGGTACCCGTGATATCGGCAACATTCGTCAAAGCAACGGTTTTAATGCCCCGAATGATGAAACTATCAGTAATGTA 831 0 541 .......................................................................................... 630 L A K G T W Q I D S I Q S L S A N L R Y Y N N S A I E P K N Query_1 832 TTGGCAAAAGGCACTTGGCAGATAGACTCGATTCAATCGTTAAGCGCCAATCTGCGTTATTACAACAACAGTGCAATAGAGCCAAAGAAT 921 0 631 .......................................................................................... 720 P Q T S A P S S T N V M T N R S T I Q R D A Q L R Y N I K P Query_1 922 CCGCAAACCAGCGCACCATCCAGTACCAATGTCATGACCAATCGTTCAACGATCCAGCGTGATGCCCAGCTCAGATATAATATTAAGCCT 1011 0 721 .......................................................................................... 810 L D Q E W L N A T A Q V Y Y S E V E I N A R P Q G S A E E G Query_1 1012 CTCGATCAAGAATGGTTGAATGCCACGGCACAGGTTTATTACTCCGAAGTGGAAATCAATGCCCGTCCACAAGGTTCAGCCGAAGAAGGC 1101 0 811 .......................................................................................... 900 R E Q T T E G V K L E N R T R L F I E S P A S H L L T Y G T Query_1 1102 CGCGAGCAGACAACCGAAGGTGTGAAGCTGGAAAACCGTACCCGTCTGTTTATCGAAAGCCCAGCCTCCCACTTGCTGACCTATGGTACT 1191 0 901 .......................................................................................... 990 E T Y K Q E Q T P G G A T E S F P Q A K I R F S S G W L Q D Query_1 1192 GAAACCTATAAACAGGAACAGACCCCCGGTGGGGCAACGGAAAGTTTCCCACAGGCCAAAATTCGGTTTAGTTCGGGCTGGCTACAAGAT 1281 0 991 .......................................................................................... 1080 E I T L R D L P V S I L A G T R Y D N Y S G S S D G Y A D V Query_1 1282 GAAATCACCTTACGCGACCTGCCTGTCTCCATATTAGCCGGTACGCGTTATGACAATTACAGTGGTAGCAGCGATGGCTATGCCGATGTC 1371 0 1081 .......................................................................................... 1170 D A D K W S S R G A I S I T P T D W L M L F G S Y A Q A F R Query_1 1372 GATGCCGATAAATGGTCATCACGTGGTGCAATCAGCATTACACCAACTGATTGGCTGATGCTGTTTGGCTCCTATGCTCAGGCTTTCCGC 1461 0 1171 .......................................................................................... 1260 A P T M G E M Y N D S K H F A I P I R P G L T L T N Y W V P Query_1 1462 GCCCCAACCATGGGTGAGATGTATAACGATTCCAAACACTTTGCGATCCCTATCCGGCCTGGGTTGACACTCACCAACTATTGGGTCCCC 1551 0 1261 .......................................................................................... 1350 N P N L K P E T N E T Q E Y G F G L R F S D L L M A E D D L Query_1 1552 AACCCAAATCTGAAACCAGAAACCAATGAGACCCAGGAATACGGTTTTGGCCTACGTTTCAGTGATTTACTGATGGCTGAAGATGACCTG 1641 0 1351 .......................................................................................... 1440 Q F K V S Y F D T K A K D Y I S T R V D M Q A M T T T S V N Query_1 1642 CAATTTAAAGTCAGTTACTTCGATACCAAGGCTAAAGACTACATCAGTACCCGCGTTGACATGCAAGCCATGACCACCACATCGGTGAAT 1731 0 1441 .......................................................................................... 1530 I D Q A K I W G W D A S M S Y K T A L F N W D L A Y N R T R Query_1 1732 ATCGATCAGGCAAAAATCTGGGGTTGGGATGCCAGCATGAGCTATAAAACGGCCTTGTTTAACTGGGATCTGGCCTATAACCGCACCCGT 1821 0 1531 .......................................................................................... 1620 0 1805 ........... 1815 G K N Q N T D E W L D T I N P D T V T S I V D V P V A N S G Query_1 1822 GGTAAAAATCAGAATACCGATGAATGGCTCGACACCATCAACCCGGATACCGTCACCAGTATCGTTGATGTACCTGTCGCCAACAGTGGT 1911 0 1621 .......................................................................................... 1710 0 275 ............ 264 F S V G W I G T F A N R S S R V S S S T P Q A G Y G V N D F Query_1 1912 TTCTCCGTCGGTTGGATTGGGACCTTCGCTAATCGCTCCAGCCGCGTTTCCAGTTCAACACCGCAAGCCGGTTATGGCGTCAATGATTTC 2001 0 1711 .......................................................................................... 1800 Y V S Y K G Q E A F K G M T T T M L L G N V F E K E Y Y T P Query_1 2002 TATGTGAGCTATAAAGGCCAGGAAGCATTCAAAGGCATGACCACAACAATGTTATTGGGTAATGTCTTCGAAAAAGAGTATTACACGCCA 2091 0 1801 .......................................................................................... 1890 0 1568 ........... 1578 Q G I P Q D G R N V K F F V S Y Q W Query_1 2092 CAAGGCATCCCACAAGATGGCCGTAACGTGAAATTTTTCGTGAGTTATCAGTGG 2145 0 1891 ...................................................... 1944 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4887635 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 2,143 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP02374.103 (IDP02374.1-868.F..pMCSG73)
MSSFVLDFQEIEKTQLSLVGGKGLNLGALSNMQGIQVPEGFCVTTAGYEKAIEQNEELQALLQQLTKLKREERVRIGEISKKIRENIMAVQIPSDVVEAVAHYLSRFGNEHAYAVRSSATAEDLPYASFAGQQDTYLNIIGENAILQHVRKCWASLFTDRAVMYRMQNGFEHNQVSICVVVQKMVFPQASGILFTADPITSNRKVLSIDASFGLGEALVSGLVSADNYKVKEGEIIDKMIATKKLAIYALKEGGTETKQNDPVQQKIQTLSDQQILQLVQIGRQIEAYFGCPQDIEWCLVDHTFYIVQSRPITTLYPIPEENDEGNHVYISVGHQQMMTDAMKPLGLSFFLLTTNAPMRKAGGRLFVDATQQLVLPASRDYLINTLGKSDPLVRDALTTIIERDDFITLLPDEEKEKSASKSKPPVSSQPEIENDPAIVTELIRNSEASLEELKQNMQLKSGVDVFDFILEDIQQLKKVLFNPQSIAVIMAGMNASTWINEKMEQWLGEKNAADMLSQSVQNNITSEMGLALMDVADAIRPYPEVITYLQHVEDDSFLDELVQYKGGEKARDTIVAFLNKYGMRCSGEIDITKTRWSEKPTTIIPMILNNIRDFEAGASKQKFEKGLQEALNKEEELLDRLQHLPKGKQKVEETKRMIRNIRNFIGYREYPKYGMINRYFIYKQALLKEAEQLVQSGVICEVDDIYYLTFEELHEVVRTNKLDYELIQKQKNDYKLYEKLTPPRVMTSDGEIITGKYKRENLPADAIVGLPVSSGVVEGRARVILNMEEANLEEGDILVTAFTDPGWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENATKRIKDGQRIRVHGTEGYIEML
ATGAGTTCTTTCGTTCTCGATTTTCAGGAAATAGAAAAAACGCAGCTTTCACTCGTTGGTGGAAAAGGTTTGAATTTAGGGGCGTTATCCAACATGCAAGGGATACAAGTGCCAGAAGGTTTTTGTGTTACAACGGCAGGATATGAAAAGGCCATCGAGCAAAATGAGGAGCTTCAAGCTTTGTTGCAACAGCTAACAAAGTTGAAACGTGAAGAGCGAGTTCGAATTGGTGAAATCAGCAAGAAGATTAGAGAAAACATTATGGCGGTACAAATCCCTTCTGATGTAGTGGAAGCAGTAGCTCATTATCTCTCACGCTTTGGAAATGAACATGCGTATGCCGTACGCTCTAGTGCGACGGCTGAAGATTTACCGTATGCCTCGTTTGCTGGTCAACAAGATACGTATTTAAATATTATCGGAGAAAACGCAATTTTACAGCATGTAAGAAAGTGCTGGGCTTCTTTATTTACAGATCGTGCAGTTATGTACCGCATGCAAAATGGTTTTGAACATAATCAAGTTTCTATATGTGTTGTCGTTCAAAAAATGGTTTTTCCGCAGGCTTCAGGAATTTTATTTACCGCTGATCCGATTACTTCTAACCGGAAGGTGTTATCAATCGATGCCAGTTTTGGACTTGGCGAGGCATTAGTATCGGGACTCGTCTCTGCCGATAATTATAAAGTAAAAGAAGGCGAAATCATCGATAAGATGATTGCAACTAAAAAATTAGCTATTTATGCTTTAAAAGAGGGCGGAACTGAGACGAAACAGAATGATCCGGTTCAGCAAAAAATTCAAACACTATCTGACCAACAAATATTACAACTAGTACAGATTGGAAGGCAGATTGAAGCTTATTTTGGCTGTCCGCAAGATATTGAATGGTGTTTAGTCGATCACACATTTTATATTGTCCAAAGTCGTCCGATAACGACTTTATATCCAATTCCGGAAGAAAATGATGAGGGAAATCATGTGTATATATCAGTTGGTCATCAACAAATGATGACCGATGCGATGAAACCACTAGGGTTATCTTTTTTCTTGTTAACGACTAATGCTCCTATGCGTAAAGCGGGAGGCAGGCTGTTTGTAGATGCTACGCAACAATTAGTTTTACCTGCTAGCAGAGATTATTTAATAAACACTTTAGGGAAATCGGATCCACTTGTAAGAGATGCATTGACGACTATAATCGAGCGAGATGATTTTATTACATTATTACCGGATGAAGAAAAAGAAAAGAGTGCTAGCAAAAGTAAGCCACCAGTAAGTTCGCAACCAGAAATCGAAAATGATCCAGCAATCGTTACGGAATTAATAAGGAATAGTGAAGCATCACTTGAAGAGTTAAAACAAAATATGCAACTGAAATCAGGAGTGGATGTATTTGATTTTATTTTGGAAGACATCCAGCAATTAAAGAAAGTATTATTTAATCCGCAAAGTATAGCTGTCATCATGGCAGGTATGAATGCTTCAACATGGATTAATGAAAAAATGGAGCAGTGGCTAGGTGAAAAGAATGCAGCAGACATGCTTTCTCAATCGGTACAAAATAATATTACATCAGAAATGGGTTTAGCATTAATGGATGTTGCTGATGCAATTCGTCCGTATCCAGAAGTTATTACGTATTTACAACATGTAGAAGATGATAGCTTTTTAGATGAACTTGTTCAGTATAAAGGCGGAGAAAAAGCTCGAGATACAATCGTTGCTTTTCTAAATAAATACGGAATGAGATGTAGCGGCGAAATCGACATTACGAAAACGCGCTGGAGTGAGAAGCCAACAACCATTATCCCAATGATATTAAATAATATAAGAGACTTTGAAGCGGGTGCTAGTAAGCAGAAGTTTGAAAAAGGACTGCAGGAAGCTTTGAATAAAGAAGAAGAGTTATTAGACCGATTGCAACATTTGCCGAAGGGTAAACAAAAAGTAGAAGAAACGAAGCGAATGATTCGTAATATCCGTAATTTTATTGGCTATCGTGAATATCCAAAATACGGCATGATTAATCGTTACTTCATATATAAGCAGGCGTTATTGAAAGAAGCTGAGCAACTTGTACAAAGCGGTGTCATTTGTGAGGTCGATGATATATACTATCTAACTTTTGAAGAACTTCATGAAGTTGTTCGCACGAATAAACTAGATTACGAGCTTATCCAAAAACAAAAAAATGATTATAAATTGTATGAAAAACTAACGCCTCCCCGCGTTATGACGTCTGATGGAGAAATCATTACAGGTAAATATAAACGAGAAAATCTCCCAGCTGACGCGATTGTAGGCCTGCCTGTTTCTTCCGGAGTAGTTGAGGGGAGAGCCCGTGTTATTTTAAACATGGAAGAAGCGAATTTAGAAGAGGGAGATATATTAGTTACTGCTTTTACTGATCCTGGCTGGACACCGTTGTTTGTTTCTATAAAAGGGTTAGTGACCGAAGTTGGTGGGCTCATGACACACGGGGCAGTGATTGCACGTGAATATGGATTACCAGCAGTTGTTGGAGTAGAGAATGCTACGAAACGAATCAAAGATGGGCAACGAATTCGGGTACATGGAACAGAAGGGTATATTGAAATGTTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,416 total letters Query= IDP02374.103 Length=3009 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4697 0.0 2_07C_C8_IDP02374.103_T7term.seq 1294 0.0 M S S F V L D F Q E I E K T Q L S L V G G K G L N L G A L S Query_1 202 ATGAGTTCTTTCGTTCTCGATTTTCAGGAAATAGAAAAAACGCAGCTTTCACTCGTTGGTGGAAAAGGTTTGAATTTAGGGGCGTTATCC 291 0 1 .......................................................................................... 90 N M Q G I Q V P E G F C V T T A G Y E K A I E Q N E E L Q A Query_1 292 AACATGCAAGGGATACAAGTGCCAGAAGGTTTTTGTGTTACAACGGCAGGATATGAAAAGGCCATCGAGCAAAATGAGGAGCTTCAAGCT 381 0 91 .......................................................................................... 180 L L Q Q L T K L K R E E R V R I G E I S K K I R E N I M A V Query_1 382 TTGTTGCAACAGCTAACAAAGTTGAAACGTGAAGAGCGAGTTCGAATTGGTGAAATCAGCAAGAAGATTAGAGAAAACATTATGGCGGTA 471 0 181 .......................................................................................... 270 Q I P S D V V E A V A H Y L S R F G N E H A Y A V R S S A T Query_1 472 CAAATCCCTTCTGATGTAGTGGAAGCAGTAGCTCATTATCTCTCACGCTTTGGAAATGAACATGCGTATGCCGTACGCTCTAGTGCGACG 561 0 271 .......................................................................................... 360 A E D L P Y A S F A G Q Q D T Y L N I I G E N A I L Q H V R Query_1 562 GCTGAAGATTTACCGTATGCCTCGTTTGCTGGTCAACAAGATACGTATTTAAATATTATCGGAGAAAACGCAATTTTACAGCATGTAAGA 651 0 361 .......................................................................................... 450 K C W A S L F T D R A V M Y R M Q N G F E H N Q V S I C V V Query_1 652 AAGTGCTGGGCTTCTTTATTTACAGATCGTGCAGTTATGTACCGCATGCAAAATGGTTTTGAACATAATCAAGTTTCTATATGTGTTGTC 741 0 451 .......................................................................................... 540 V Q K M V F P Q A S G I L F T A D P I T S N R K V L S I D A Query_1 742 GTTCAAAAAATGGTTTTTCCGCAGGCTTCAGGAATTTTATTTACCGCTGATCCGATTACTTCTAACCGGAAGGTGTTATCAATCGATGCC 831 0 541 .......................................................................................... 630 S F G L G E A L V S G L V S A D N Y K V K E G E I I D K M I Query_1 832 AGTTTTGGACTTGGCGAGGCATTAGTATCGGGACTCGTCTCTGCCGATAATTATAAAGTAAAAGAAGGCGAAATCATCGATAAGATGATT 921 0 631 .......................................................................................... 720 A T K K L A I Y A L K E G G T E T K Q N D P V Q Q K I Q T L Query_1 922 GCAACTAAAAAATTAGCTATTTATGCTTTAAAAGAGGGCGGAACTGAGACGAAACAGAATGATCCGGTTCAGCAAAAAATTCAAACACTA 1011 0 721 .......................................................................................... 810 S D Q Q I L Q L V Q I G R Q I E A Y F G C P Q D I E W C L V Query_1 1012 TCTGACCAACAAATATTACAACTAGTACAGATTGGAAGGCAGATTGAAGCTTATTTTGGCTGTCCGCAAGATATTGAATGGTGTTTAGTC 1101 0 811 .......................................................................................... 900 D H T F Y I V Q S R P I T T L Y P I P E E N D E G N H V Y I Query_1 1102 GATCACACATTTTATATTGTCCAAAGTCGTCCGATAACGACTTTATATCCAATTCCGGAAGAAAATGATGAGGGAAATCATGTGTATATA 1191 0 901 .......................................................................................... 990 0 2325 ........... 2315 2 354 ........... 364 S V G H Q Q M M T D A M K P L G L S F F L L T T N A P M R K Query_1 1192 TCAGTTGGTCATCAACAAATGATGACCGATGCGATGAAACCACTAGGGTTATCTTTTTTCTTGTTAACGACTAATGCTCCTATGCGTAAA 1281 0 991 .......................................................................................... 1080 A G G R L F V D A T Q Q L V L P A S R D Y L I N T L G K S D Query_1 1282 GCGGGAGGCAGGCTGTTTGTAGATGCTACGCAACAATTAGTTTTACCTGCTAGCAGAGATTATTTAATAAACACTTTAGGGAAATCGGAT 1371 0 1081 .......................................................................................... 1170 0 1830 ........... 1820 P L V R D A L T T I I E R D D F I T L L P D E E K E K S A S Query_1 1372 CCACTTGTAAGAGATGCATTGACGACTATAATCGAGCGAGATGATTTTATTACATTATTACCGGATGAAGAAAAAGAAAAGAGTGCTAGC 1461 0 1171 .......................................................................................... 1260 K S K P P V S S Q P E I E N D P A I V T E L I R N S E A S L Query_1 1462 AAAAGTAAGCCACCAGTAAGTTCGCAACCAGAAATCGAAAATGATCCAGCAATCGTTACGGAATTAATAAGGAATAGTGAAGCATCACTT 1551 0 1261 .......................................................................................... 1350 E E L K Q N M Q L K S G V D V F D F I L E D I Q Q L K K V L Query_1 1552 GAAGAGTTAAAACAAAATATGCAACTGAAATCAGGAGTGGATGTATTTGATTTTATTTTGGAAGACATCCAGCAATTAAAGAAAGTATTA 1641 0 1351 .......................................................................................... 1440 0 1831 ..... 1827 F N P Q S I A V I M A G M N A S T W I N E K M E Q W L G E K Query_1 1642 TTTAATCCGCAAAGTATAGCTGTCATCATGGCAGGTATGAATGCTTCAACATGGATTAATGAAAAAATGGAGCAGTGGCTAGGTGAAAAG 1731 0 1441 .......................................................................................... 1530 0 1826 ...... 1821 N A A D M L S Q S V Q N N I T S E M G L A L M D V A D A I R Query_1 1732 AATGCAGCAGACATGCTTTCTCAATCGGTACAAAATAATATTACATCAGAAATGGGTTTAGCATTAATGGATGTTGCTGATGCAATTCGT 1821 0 1531 .......................................................................................... 1620 P Y P E V I T Y L Q H V E D D S F L D E L V Q Y K G G E K A Query_1 1822 CCGTATCCAGAAGTTATTACGTATTTACAACATGTAGAAGATGATAGCTTTTTAGATGAACTTGTTCAGTATAAAGGCGGAGAAAAAGCT 1911 0 1621 .......................................................................................... 1710 R D T I V A F L N K Y G M R C S G E I D I T K T R W S E K P Query_1 1912 CGAGATACAATCGTTGCTTTTCTAAATAAATACGGAATGAGATGTAGCGGCGAAATCGACATTACGAAAACGCGCTGGAGTGAGAAGCCA 2001 0 1711 .......................................................................................... 1800 T T I I P M I L N N I R D F E A G A S K Q K F E K G L Q E A Query_1 2002 ACAACCATTATCCCAATGATATTAAATAATATAAGAGACTTTGAAGCGGGTGCTAGTAAGCAGAAGTTTGAAAAAGGACTGCAGGAAGCT 2091 0 1801 .......................................................................................... 1890 0 1149 ........... 1139 0 1446 ........... 1436 2 807 ...N.NN........... 790 L N K E E E L L D R L Q H L P K G K Q K V E E T K R M I R Query_1 2092 TTGAATAAAGAAGAAGAGTTATTAGACCGATTGCAACATTTGCCGAAGGG-TAAACAAAAAGTAGAAGAAACGAAGCGAATGATTCGTAA 2180 0 1891 ..................................................-....................................... 1979 2 789 ..................................................G...N................................... 700 N I R N F I G Y R E Y P K Y G M I N R Y F I Y K Q A L L K E Query_1 2181 TATCCGTAATTTTATTGGCTATCGTGAATATCCAAAATACGGCATGATTAATCGTTACTTCATATATAAGCAGGCGTTATTGAAAGAAGC 2270 0 1980 .......................................................................................... 2069 2 699 ...................N.....................N................................................ 610 A E Q L V Q S G V I C E V D D I Y Y L T F E E L H E V V R T Query_1 2271 TGAGCAACTTGTACAAAGCGGTGTCATTTGTGAGGTCGATGATATATACTATCTAACTTTTGAAGAACTTCATGAAGTTGTTCGCACGAA 2360 0 2070 .......................................................................................... 2159 0 2152 ...C..............C... 2131 2 609 .......................................................................................... 520 2 527 ...C..............C... 548 N K L D Y E L I Q K Q K N D Y K L Y E K L T P P R V M T S D Query_1 2361 TAAACTAGATTACGAGCTTATCCaaaaacaaaaaaaTGATTATAAATTGTATGAAAAACTAACGCCTCCCCGCGTTATGACGTCTGATGG 2450 0 2160 .......................................................................................... 2249 2 519 .......................................................................................... 430 G E I I T G K Y K R E N L P A D A I V G L P V S S G V V E G Query_1 2451 AGAAATCATTACAGGTAAATATAAACGAGAAAATCTCCCAGCTGACGCGATTGTAGGCCTGCCTGTTTCTTCCGGAGTAGTTGAGGGGAG 2540 0 2250 .......................................................................................... 2339 0 964 ........... 954 2 429 .......................................................................................... 340 R A R V I L N M E E A N L E E G D I L V T A F T D P G W T P Query_1 2541 AGCCCGTGTTATTTTAAACATGGAAGAAGCGAATTTAGAAGAGGGAGATATATTAGTTACTGCTTTTACTGATCCTGGCTGGACACCGTT 2630 0 2340 .......................................................................................... 2429 2 339 .......................................................................................... 250 L F V S I K G L V T E V G G L M T H G A V I A R E Y G L P A Query_1 2631 GTTTGTTTCTATAAAAGGGTTAGTGACCGAAGTTGGTGGGCTCATGACACACGGGGCAGTGATTGCACGTGAATATGGATTACCAGCAGT 2720 0 2430 .......................................................................................... 2519 2 249 .......................................................................................... 160 V V G V E N A T K R I K D G Q R I R V H G T E G Y I E M L * Query_1 2721 TGTTGGAGTAGAGAATGCTACGAAACGAATCAAAGATGGGCAACGAATTCGGGTACATGGAACAGAAGGGTATATTGAAATGTTGTAACA 2810 0 2520 ..................................................................................... 2604 2 159 .....................................................................................C.T.. 70 H W K W I T D P N S S S V D K L A A A L E H H H H H H * D P Query_1 2811 TTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGC 2900 2 69 . 69 A A N K A R K E A E L A A A T A E Q * L A * P L G A S K R V Query_1 2901 TGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTT 2990 L R G F L L Query_1 2991 GAGGGGTTTTTTGCTGAA 3008 0 799 ............ 788 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 10068080 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 3,416 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP05031.103 (IDP05031.22-528.P(528)..pMCSG73)
SGEKDSKQAGTSKSGIPKDGGTLTIGVSDNPETMNPLYANDRVSLTVQQALYAPLYHMEDGKKKFVLAESFTPSEDQLTWTLKLKDNLKWHDGKKITSDDIAFTFQSILDEKQNSSSRENFVFKGKPLEVKKVDELTTQFVLPQVSASFEGVMNDFFPIPKHVFEGEADLAKSKKNLQPVGSGPFKFKEYKSDEYVALDRFDDYVGGKAKLDSIVYRVVKDRNTANVSLQNGQINMKMIEPQDFKKLDSTGNFSMVTFPEGRLFYLSYNMNTDLMKKKEVRQAIAHALDKKEMINSAFVSSQFAEPANSILTPDAMYYAKDIKEYKYDKKEAKDLLAKAGVKDKEKVRVMYVTNNKIMESLALYTQQKLQEVGLEVELNALDASAASDKSLDKENKEYDITFGGYIMGPEPDSYKSLFLSNAEYNYARYKNADFDKLWEEAAVETDKTKRAELYHKIQETAREDLPYLPIAYPKAVIAVDKKFDGLKEAKAIPVTMFEDVSKIYEVK
TCAGGGGAAAAAGATTCGAAGCAAGCAGGAACTAGTAAATCAGGGATTCCGAAAGATGGAGGAACGTTAACGATTGGTGTTAGCGATAACCCAGAGACGATGAATCCGCTTTATGCGAATGATCGTGTTTCGTTAACGGTGCAGCAAGCTTTATATGCACCGCTGTATCATATGGAAGACGGTAAGAAGAAGTTTGTTCTTGCTGAGAGCTTTACGCCTTCAGAGGATCAATTAACATGGACTTTAAAACTAAAGGATAATTTGAAATGGCATGACGGTAAGAAGATTACATCAGACGACATAGCATTTACATTCCAATCTATTTTAGATGAGAAGCAAAATAGCTCAAGTCGTGAAAACTTTGTTTTTAAAGGAAAACCGCTTGAGGTGAAAAAGGTAGATGAGTTAACAACTCAATTCGTTTTACCACAAGTAAGTGCATCTTTCGAAGGTGTGATGAATGATTTCTTCCCAATTCCGAAACATGTATTTGAAGGGGAAGCAGATTTAGCGAAGAGTAAGAAAAACTTACAGCCTGTAGGATCAGGTCCGTTTAAGTTTAAAGAGTATAAGTCTGATGAGTACGTTGCATTAGATCGATTTGATGATTATGTAGGTGGTAAAGCTAAATTAGATTCTATCGTATACCGCGTTGTAAAAGATCGTAATACAGCAAATGTTTCACTACAAAATGGTCAAATCAACATGAAGATGATTGAGCCACAAGACTTTAAGAAATTAGATAGTACAGGGAACTTCTCAATGGTGACATTCCCAGAAGGTAGATTATTCTACTTATCTTATAACATGAATACAGATCTGATGAAGAAAAAAGAAGTGCGCCAAGCAATTGCACATGCGTTAGATAAAAAAGAAATGATTAACTCAGCATTCGTTTCTAGTCAATTTGCAGAACCAGCAAATTCAATTTTAACGCCAGACGCTATGTATTATGCAAAAGACATTAAAGAGTATAAGTATGATAAAAAAGAAGCGAAAGATTTATTAGCGAAAGCTGGCGTGAAGGATAAAGAAAAAGTACGCGTTATGTATGTAACGAATAATAAAATTATGGAAAGCTTAGCGCTTTATACACAACAAAAATTACAAGAAGTTGGCTTAGAAGTTGAACTGAATGCATTAGATGCTAGTGCAGCAAGTGACAAAAGCTTAGATAAAGAGAATAAAGAATATGACATTACATTCGGTGGTTACATAATGGGACCTGAGCCAGATTCATATAAGAGCTTATTCTTAAGTAATGCTGAATACAACTATGCACGTTATAAAAACGCTGATTTCGATAAGTTATGGGAAGAAGCTGCAGTTGAAACAGATAAAACAAAACGCGCAGAACTATATCATAAAATTCAAGAGACAGCTAGAGAAGACTTACCTTATCTACCAATTGCGTATCCGAAAGCAGTTATTGCAGTAGATAAAAAGTTTGATGGATTGAAAGAAGCAAAAGCAATTCCTGTCACAATGTTTGAAGATGTATCTAAGATTTATGAAGTGAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 1,531 total letters Query= IDP05031.103 Length=1926 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2744 0.0 S G E K D S K Q A G T S K S G I P K D G G T L T I G V S D N Query_1 202 TCAGGGGAAAAAGATTCGAAGCAAGCAGGAACTAGTAAATCAGGGATTCCGAAAGATGGAGGAACGTTAACGATTGGTGTTAGCGATAAC 291 0 1 .......................................................................................... 90 P E T M N P L Y A N D R V S L T V Q Q A L Y A P L Y H M E D Query_1 292 CCAGAGACGATGAATCCGCTTTATGCGAATGATCGTGTTTCGTTAACGGTGCAGCAAGCTTTATATGCACCGCTGTATCATATGGAAGAC 381 0 91 .......................................................................................... 180 0 274 ... 276 G K K K F V L A E S F T P S E D Q L T W T L K L K D N L K W Query_1 382 GGTAAGAAGAAGTTTGTTCTTGCTGAGAGCTTTACGCCTTCAGAGGATCAATTAACATGGACTTTAAAACTAAAGGATAATTTGAAATGG 471 0 181 .......................................................................................... 270 0 277 .......... 286 H D G K K I T S D D I A F T F Q S I L D E K Q N S S S R E N Query_1 472 CATGACGGTAAGAAGATTACATCAGACGACATAGCATTTACATTCCAATCTATTTTAGATGAGAAGCAAAATAGCTCAAGTCGTGAAAAC 561 0 271 .......................................................................................... 360 0 178 ............. 190 F V F K G K P L E V K K V D E L T T Q F V L P Q V S A S F E Query_1 562 TTTGTTTTTAAAGGAAAACCGCTTGAGGTGAAAAAGGTAGATGAGTTAACAACTCAATTCGTTTTACCACAAGTAAGTGCATCTTTCGAA 651 0 361 .......................................................................................... 450 G V M N D F F P I P K H V F E G E A D L A K S K K N L Q P V Query_1 652 GGTGTGATGAATGATTTCTTCCCAATTCCGAAACATGTATTTGAAGGGGAAGCAGATTTAGCGAAGAGTAAGAAAAACTTACAGCCTGTA 741 0 451 .......................................................................................... 540 G S G P F K F K E Y K S D E Y V A L D R F D D Y V G G K A K Query_1 742 GGATCAGGTCCGTTTAAGTTTAAAGAGTATAAGTCTGATGAGTACGTTGCATTAGATCGATTTGATGATTATGTAGGTGGTAAAGCTAAA 831 0 541 .......................................................................................... 630 0 965 ...............A.... 984 L D S I V Y R V V K D R N T A N V S L Q N G Q I N M K M I E Query_1 832 TTAGATTCTATCGTATACCGCGTTGTAAAAGATCGTAATACAGCAAATGTTTCACTACAAAATGGTCAAATCAACATGAAGATGATTGAG 921 0 631 .......................................................................................... 720 P Q D F K K L D S T G N F S M V T F P E G R L F Y L S Y N M Query_1 922 CCACAAGACTTTAAGAAATTAGATAGTACAGGGAACTTCTCAATGGTGACATTCCCAGAAGGTAGATTATTCTACTTATCTTATAACATG 1011 0 721 .......................................................................................... 810 N T D L M K K K E V R Q A I A H A L D K K E M I N S A F V S Query_1 1012 AATACAGATCTGATGAAGAAAAAAGAAGTGCGCCAAGCAATTGCACATGCGTTAGATAAAAAAGAAATGATTAACTCAGCATTCGTTTCT 1101 0 811 .......................................................................................... 900 0 982 ............ 993 S Q F A E P A N S I L T P D A M Y Y A K D I K E Y K Y D K K Query_1 1102 AGTCAATTTGCAGAACCAGCAAATTCAATTTTAACGCCAGACGCTATGTATTATGCAAAAGACATTAAAGAGTATAAGTATGATAAAAAA 1191 0 901 .......................................................................................... 990 0 560 ...............C.... 579 0 865 ......... 873 E A K D L L A K A G V K D K E K V R V M Y V T N N K I M E S Query_1 1192 GAAGCGAAAGATTTATTAGCGAAAGCTGGCGTGAAGGATAAAGAAAAAGTACGCGTTATGTATGTAACGAATAATAAAATTATGGAAAGC 1281 0 991 .......................................................................................... 1080 0 874 ... 876 L A L Y T Q Q K L Q E V G L E V E L N A L D A S A A S D K S Query_1 1282 TTAGCGCTTTATACACAACAAAAATTACAAGAAGTTGGCTTAGAAGTTGAACTGAATGCATTAGATGCTAGTGCAGCAAGTGACAAAAGC 1371 0 1081 .......................................................................................... 1170 L D K E N K E Y D I T F G G Y I M G P E P D S Y K S L F L S Query_1 1372 TTAGATAAAGAGAATAAAGAATATGACATTACATTCGGTGGTTACATAATGGGACCTGAGCCAGATTCATATAAGAGCTTATTCTTAAGT 1461 0 1171 .......................................................................................... 1260 N A E Y N Y A R Y K N A D F D K L W E E A A V E T D K T K R Query_1 1462 AATGCTGAATACAACTATGCACGTTATAAAAACGCTGATTTCGATAAGTTATGGGAAGAAGCTGCAGTTGAAACAGATAAAACAAAACGC 1551 0 1261 .......................................................................................... 1350 A E L Y H K I Q E T A R E D L P Y L P I A Y P K A V I A V D Query_1 1552 GCAGAACTATATCATAAAATTCAAGAGACAGCTAGAGAAGACTTACCTTATCTACCAATTGCGTATCCGAAAGCAGTTATTGCAGTAGAT 1641 0 1351 .......................................................................................... 1440 K K F D G L K E A K A I P V T M F E D V S K I Y E V K Query_1 1642 AAAAAGTTTGATGGATTGAAAGAAGCAAAAGCAATTCCTGTCACAATGTTTGAAGATGTATCTAAGATTTATGAAGTGAAA 1722 0 1441 ................................................................................. 1521 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2839746 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 1,531 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP05160.103 (IDP05160.25-256.P(256)..pMCSG73)
KAHTNKVAIVIDDFGNNMKGTDKMLSLPIPLTVAVMPFLPSTKEDAIAAHKKGHEVIIHMPMEPIKGKKEWLGPKAITTDLSDEEINNRLEQAIQEVPHAIGMNNHMGSKVTADERIVRLILAACKKHGLFYLDSKTNPKSVVPKIGKELGVPIIENQLFFDDVYTAAHISKQAQLLIKKLQEKPIMVAIGHVGPPGEITSRVIETSIPKIREHADFIFLSDLALSPPPVSK
AAGGCCCATACAAACAAAGTAGCTATTGTCATTGATGACTTCGGCAATAATATGAAGGGGACTGATAAAATGTTATCACTTCCTATTCCCCTAACTGTTGCTGTTATGCCTTTTCTTCCTTCCACAAAGGAAGATGCGATAGCGGCTCATAAGAAAGGACACGAAGTTATTATACATATGCCAATGGAACCCATTAAAGGTAAAAAAGAGTGGCTTGGACCAAAAGCAATTACAACTGATTTAAGCGACGAAGAAATAAACAATCGACTCGAACAAGCAATTCAAGAAGTACCACATGCAATTGGGATGAATAATCATATGGGATCAAAAGTGACTGCAGACGAAAGAATTGTACGACTTATACTTGCAGCTTGTAAAAAACACGGCTTATTTTATTTAGATAGCAAAACAAACCCTAAAAGTGTCGTCCCAAAAATTGGGAAAGAATTAGGGGTGCCAATTATTGAAAATCAATTATTTTTTGATGATGTGTATACTGCGGCACATATTTCAAAACAAGCCCAATTACTCATAAAAAAACTTCAAGAAAAACCGATTATGGTGGCTATTGGACATGTTGGCCCTCCAGGTGAAATTACATCACGTGTTATAGAAACTTCTATTCCTAAAATTCGTGAACACGCAGATTTTATTTTTTTATCTGATTTAGCATTATCTCCACCTCCTGTTTCGAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,292 total letters Query= IDP05160.103 Length=1101 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1256 0.0 2_06H_H7_IDP05160.103_T7term.seq 1038 0.0 1_04H_H7_IDP05160.103_v73F.seq 726 0.0 I H M H H H H H H S S G V D L W S H P Q F E K G T E N L Y Query_1 99 TATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTT 188 0 171 ........... 181 2 593 ........... 583 1 67 ..G....... 76 F Q S N A K A H T N K V A I V I D D F G N N M K G T D K M L Query_1 189 CCAATCCAATGCCAAGGCCCATACAAACAAAGTAGCTATTGTCATTGATGACTTCGGCAATAATATGAAGGGGACTGATAAAATGTTATC 278 0 1 ............................................................................. 77 1 77 .T.TC..--.C..CC.....TN....CT.C.C.GA...................T...N..C-..C.C...........TC.CC...... 163 S L P I P L T V A V M P F L P S T K E D A I A A H K K G H E Query_1 279 ACTTCCTATTCCCCTAACTGTTGCTGTTATGCCTTTTCTTCCTTCCACAAAGGAAGATGCGATAGCGGCT-CATAAGAAAGGACACGAAG 367 0 78 ......................................................................-................... 166 2 647 ........................NNNAAAAN.................. 598 1 164 .....T..............N..........T...........N..TN..-....NC.........C...-.....C......T-....C 250 V I I H M P M E P I K G K K E W L G P K A I T T D L S D E E Query_1 368 TTATTATACATATGCCAA-TGGAACCCATTAAAGGTAAAAAAGAGTGGCTTGGACCAAAAGCAATTACAACTGATTTAAGCGACGAAGAA 456 0 167 ..................-....................................................................... 255 2 597 ..................A....................................................................... 508 1 251 ........T.-..T....-....T.....N............C...........T..N....C......N.........T...T...... 338 I N N R L E Q A I Q E V P H A I G M N N H M G S K V T A D E Query_1 457 ATAAACAATCGACTCGAACAAGCAATTCAAGAAGTACCACATGCAATTGGGATGAATAATCATATGGGATCAAAAGTGACTGCAGACGAA 546 0 256 .......................................................................................... 345 2 507 .......................................................................................... 418 1 339 ..T.T..T.T.....CCC...ANT-..........C.NG.............A....C...C..N..T...............NC..A.. 427 R I V R L I L A A C K K H G L F Y L D S K T N P K S V V P K Query_1 547 AGAATTGTACGACTTATACTTGCAGCTTGTAAAAAACACGGCTTATTTTATTTAGATAGCAAAACAAACCCTAAAAGTGTCGTCCCAAAA 636 0 346 .......................................................................................... 435 2 417 ............N............................................................................. 328 1 428 N.........N............N....T.N.....TT-..N......C.......C.T-..........................N... 515 I G K E L G V P I I E N Q L F F D D V Y T A A H I S K Q A Q Query_1 637 ATTGGGAAAGAATTAGGGGTGCCAATTATTGAAAATCAATTATTTTTTGATGATGTGTATACTGCGGCACATATTTCAAAACAAGCCCAA 726 0 436 .......................................................................................... 525 2 327 .......................................................................................... 238 1 516 .........A...CCA..-......N..C............................N.......C................TT.N.TN. 604 L L I K K L Q E K P I M V A I G H V G P P G E I T S R V I E Query_1 727 TTACTCATaaaaaaaCTTCAAGAAAAACCGATTATGGTGGCTATTGGACATGTTGGCCCTCCAGGTGAAATTACATCACGTGTTATAGAA 816 0 526 .......................................................................................... 615 2 237 .......................................................................................... 148 1 605 .N.................N-.........................N....N...........N.C................N...CCC. 693 T S I P K I R E H A D F I F L S D L A L S P P P V S K * H Query_1 817 ACTTCTATTCCTAAAATTCGTGAACACGCAGATTTTA--tttttttATCTGATTTAGCATTATCTCCACCTCCTG-TTTCGAAATAACAT 903 0 616 .....................................--....................................-........ 696 2 147 .....................................--....................................-.............. 61 1 694 ..................NNC...NT.N....AC...NT.........G..................N.......T....A...N..... 783 W K W I T D P N S S S V D K L A A A L E H H H H H H * D P Query_1 904 TGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGG-C 992 2 60 ..............--..... 42 2 46 ......G. 39 1 784 .. 785 A A N K A R K E A E L A A A T A E Q * L A * P L G A S Query_1 993 TGCTAACAAAGCC-CG-AAAGGAAG-CTGAG--TTGGCTGCT-GCCACCGCTG-AGCAATAACTAGCATAACCCC-TTGGGGCC-TCTAA 1073 2 38 .............A..A....... 15 1 812 ......NN.N..-..T....AT....TAT.......T.T.........NN.N.N......N..N.......C........N..... 896 K R V L R G F L Query_1 1074 ACGGGTCT-TGAGGGGTTTTTTG 1095 1 897 .....A..A..GA.......... 919 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2440242 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 2,292 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP05180.102 (IDP05180.24-568.P(568)..pMCSG68)
ACSGSGGEKKSTTTSSGGGEEKKSEIKYAAKQVLNRTENQEIPTMDVSKSTDTLGSQILGNTMEGLYRLDKDNKPIPAAAESSTKSEDGKKYTFKLRKDAKWSNGDPVTAKDFVYAWQRLLDKNTAAEYAFIAYYIKNAEAINKGEKPLTDLGAKAVDDYTLEVELEKPVPYFLNLMAFPSYYPLNEKFVKEKGDKFGLEADTTLYNGPFVMASWKHEQGWQLKKNDKYWDNKTVKLEEINYSVVKEVATKVNLYDTGSIDFTLLSGEFVDKYKSNKEEYGEYSEASTFFLRLNQKRNGQDTPLKSKKLREAIALSIDKKGLATVILNNGSKATDQLVPKGLATGPDGKDYQDTFKNGLKYDPKKGAAAWEEAKKELGKDQVTIELLSYDDGTAKKIADYFKDQIEKNLKGVTVNTKIQPFKQKLKLESAQDYEVSFAGWSPDYSDPMTFIDMFESKSPYNQMSYSNPKYDEMVAKAGNELLSDPKKRWETLGKAEKLFLEEDAGLVPLYQTGRAYVMKPNVKGIVKHNISPEYSFKWAYVTEGK
GCTTGTTCTGGATCTGGTGGGGAGAAGAAATCAACTACGACGTCTAGTGGTGGTGGGGAAGAGAAAAAGTCTGAAATTAAATACGCAGCGAAACAAGTGTTAAATCGTACAGAGAATCAAGAGATTCCGACGATGGATGTTTCAAAATCTACCGATACATTAGGTTCTCAAATTTTAGGGAACACGATGGAAGGTTTATATCGATTAGATAAAGATAATAAGCCAATCCCAGCTGCAGCAGAATCTAGTACGAAAAGCGAGGATGGCAAAAAATATACATTTAAATTACGTAAAGATGCAAAATGGTCAAATGGTGATCCTGTAACAGCGAAAGATTTCGTATATGCATGGCAGCGCTTACTTGATAAAAATACAGCGGCAGAATATGCATTTATTGCTTACTATATTAAAAACGCAGAGGCAATTAATAAAGGTGAAAAACCACTAACAGATTTAGGAGCAAAAGCGGTAGATGATTATACGCTAGAAGTAGAATTAGAGAAACCAGTACCATATTTCTTGAATTTAATGGCATTCCCATCTTACTATCCTTTAAATGAAAAGTTCGTAAAAGAAAAAGGAGATAAATTCGGTTTAGAAGCAGATACAACGTTGTATAACGGACCGTTCGTTATGGCTTCATGGAAACATGAACAAGGATGGCAGCTAAAGAAAAATGATAAGTACTGGGATAATAAGACTGTAAAATTAGAAGAAATTAACTATAGTGTAGTAAAAGAAGTTGCGACGAAAGTAAACTTATATGATACAGGATCAATTGATTTCACGTTATTATCAGGAGAATTCGTTGATAAATATAAATCGAACAAAGAAGAGTACGGCGAGTATTCGGAAGCAAGTACATTCTTCTTACGTTTAAATCAAAAGCGTAACGGACAAGATACACCGTTAAAGAGCAAAAAACTTCGTGAAGCGATCGCATTATCAATTGATAAAAAAGGATTAGCAACCGTTATTTTAAATAACGGTTCAAAAGCAACAGATCAATTAGTACCAAAAGGGCTTGCGACAGGACCAGACGGTAAAGACTACCAAGATACGTTTAAAAATGGTCTAAAATATGATCCGAAAAAAGGTGCAGCAGCTTGGGAAGAAGCGAAAAAAGAACTTGGAAAAGATCAAGTGACAATTGAATTACTAAGCTATGATGATGGAACTGCGAAAAAAATTGCTGACTACTTTAAAGATCAAATTGAGAAAAACTTAAAAGGTGTAACGGTTAACACGAAAATTCAACCGTTCAAACAAAAACTAAAATTAGAGTCAGCACAAGATTATGAAGTTTCGTTTGCAGGTTGGAGTCCAGATTATTCGGATCCAATGACATTTATTGATATGTTTGAATCGAAGAGCCCATATAACCAAATGAGTTATTCGAATCCAAAATATGATGAAATGGTAGCGAAAGCAGGTAATGAATTACTGTCTGATCCGAAGAAGCGTTGGGAAACGTTAGGAAAAGCAGAGAAATTATTCCTTGAAGAAGATGCAGGATTAGTTCCTTTATATCAAACAGGAAGAGCGTATGTAATGAAACCGAATGTAAAAGGAATTGTGAAACATAACATTAGTCCAGAATATAGCTTTAAGTGGGCTTATGTAACGGAAGGTAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,241 total letters Query= IDP05180.102 Length=2040 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2949 0.0 2_08B_B2_IDP05180.102_T7term.seq 978 0.0 A C S G S G G E K K S T T T S S G G G E E K K S E I K Y A A Query_1 202 GCTTGTTCTGGATCTGGTGGGGAGAAGAAATCAACTACGACGTCTAGTGGTGGTGGGGAAGAGAAAAAGTCTGAAATTAAATACGCAGCG 291 0 1 .......................................................................................... 90 K Q V L N R T E N Q E I P T M D V S K S T D T L G S Q I L G Query_1 292 AAACAAGTGTTAAATCGTACAGAGAATCAAGAGATTCCGACGATGGATGTTTCAAAATCTACCGATACATTAGGTTCTCAAATTTTAGGG 381 0 91 .......................................................................................... 180 N T M E G L Y R L D K D N K P I P A A A E S S T K S E D G K Query_1 382 AACACGATGGAAGGTTTATATCGATTAGATAAAGATAATAAGCCAATCCCAGCTGCAGCAGAATCTAGTACGAAAAGCGAGGATGGCAAA 471 0 181 .......................................................................................... 270 K Y T F K L R K D A K W S N G D P V T A K D F V Y A W Q R L Query_1 472 AAATATACATTTAAATTACGTAAAGATGCAAAATGGTCAAATGGTGATCCTGTAACAGCGAAAGATTTCGTATATGCATGGCAGCGCTTA 561 0 271 .......................................................................................... 360 L D K N T A A E Y A F I A Y Y I K N A E A I N K G E K P L T Query_1 562 CTTGATAAAAATACAGCGGCAGAATATGCATTTATTGCTTACTATATTAAAAACGCAGAGGCAATTAATAAAGGTGAAAAACCACTAACA 651 0 361 .......................................................................................... 450 D L G A K A V D D Y T L E V E L E K P V P Y F L N L M A F P Query_1 652 GATTTAGGAGCAAAAGCGGTAGATGATTATACGCTAGAAGTAGAATTAGAGAAACCAGTACCATATTTCTTGAATTTAATGGCATTCCCA 741 0 451 .......................................................................................... 540 S Y Y P L N E K F V K E K G D K F G L E A D T T L Y N G P F Query_1 742 TCTTACTATCCTTTAAATGAAAAGTTCGTAAAAGAAAAAGGAGATAAATTCGGTTTAGAAGCAGATACAACGTTGTATAACGGACCGTTC 831 0 541 .......................................................................................... 630 0 618 .............. 605 V M A S W K H E Q G W Q L K K N D K Y W D N K T V K L E E I Query_1 832 GTTATGGCTTCATGGAAACATGAACAAGGATGGCAGCTAAAGAAAAATGATAAGTACTGGGATAATAAGACTGTAAAATTAGAAGAAATT 921 0 631 .......................................................................................... 720 N Y S V V K E V A T K V N L Y D T G S I D F T L L S G E F V Query_1 922 AACTATAGTGTAGTAAAAGAAGTTGCGACGAAAGTAAACTTATATGATACAGGATCAATTGATTTCACGTTATTATCAGGAGAATTCGTT 1011 0 721 .......................................................................................... 810 D K Y K S N K E E Y G E Y S E A S T F F L R L N Q K R N G Q Query_1 1012 GATAAATATAAATCGAACAAAGAAGAGTACGGCGAGTATTCGGAAGCAAGTACATTCTTCTTACGTTTAAATCAAAAGCGTAACGGACAA 1101 0 811 .......................................................................................... 900 D T P L K S K K L R E A I A L S I D K K G L A T V I L N N G Query_1 1102 GATACACCGTTAAAGAGCAAAAAACTTCGTGAAGCGATCGCATTATCAATTGATAAAAAAGGATTAGCAACCGTTATTTTAAATAACGGT 1191 0 901 .......................................................................................... 990 0 991 ...........A.......... 970 0 956 .............. 943 S K A T D Q L V P K G L A T G P D G K D Y Q D T F K N G L K Query_1 1192 TCAAAAGCAACAGATCAATTAGTACCAAAAGGGCTTGCGACAGGACCAGACGGTAAAGACTACCAAGATACGTTTAAAAATGGTCTAAAA 1281 0 991 .......................................................................................... 1080 0 969 . 969 Y D P K K G A A A W E E A K K E L G K D Q V T I E L L S Y D Query_1 1282 TATGATCCGAAAAAAGGTGCAGCAGCTTGGGAAGAAGCGAAAAAAGAACTTGGAAAAGATCAAGTGACAATTGAATTACTAAGCTATGAT 1371 0 1081 .......................................................................................... 1170 2 601 ......A 595 D G T A K K I A D Y F K D Q I E K N L K G V T V N T K I Q P Query_1 1372 GATGGAACTGCGaaaaaaaTTGCTGACTACTTTAAAGATCAAATTGAGAAAAACTTAAAAGGTGTAACGGTTAACACGAAAATTCAACCG 1461 0 1171 .......................................................................................... 1260 2 594 ..AA...N..............N...N.....N..-........................................N..........A.. 506 F K Q K L K L E S A Q D Y E V S F A G W S P D Y S D P M T F Query_1 1462 TTCAAACAAAAACTAAAATTAGAGTCAGCACAAGATTATGAAGTTTCGTTTGCAGGTTGGAGTCCAGATTATTCGGATCCAATGACATTT 1551 0 1261 .......................................................................................... 1350 2 505 ..............................................N...N.....N................................. 416 I D M F E S K S P Y N Q M S Y S N P K Y D E M V A K A G N E Query_1 1552 ATTGATATGTTTGAATCGAAGAGCCCATATAACCAAATGAGTTATTCGAATCCAAAATATGATGAAATGGTAGCGAAAGCAGGTAATGAA 1641 0 1351 .......................................................................................... 1440 2 415 .......N...............................................................CA................. 326 L L S D P K K R W E T L G K A E K L F L E E D A G L V P L Y Query_1 1642 TTACTGTCTGATCCGAAGAAGCGTTGGGAAACGTTAGGAAAAGCAGAGAAATTATTCCTTGAAGAAGATGCAGGATTAGTTCCTTTATAT 1731 0 1441 .......................................................................................... 1530 2 325 .......................................................................................... 236 Q T G R A Y V M K P N V K G I V K H N I S P E Y S F K W A Y Query_1 1732 CAAACAGGAAGAGCGTATGTAATGAAACCGAATGTAAAAGGAATTGTGAAACATAACATTAGTCCAGAATATAGCTTTAAGTGGGCTTAT 1821 0 1531 .......................................................................................... 1620 2 235 .......................................................................................... 146 V T E G K * H W K W I T D P N S S S V D K L A A A L E H H H Query_1 1822 GTAACGGAAGGTAAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccac 1911 0 1621 ............... 1635 2 145 ..............................................................A.A......................... 56 H H H * D P A A N K A R K E Query_1 1912 caccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAA 1953 2 55 ........N.........................N....... 14 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4438632 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 2,241 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP05180.103 (IDP05180.24-568.P(568)..pMCSG73)
ACSGSGGEKKSTTTSSGGGEEKKSEIKYAAKQVLNRTENQEIPTMDVSKSTDTLGSQILGNTMEGLYRLDKDNKPIPAAAESSTKSEDGKKYTFKLRKDAKWSNGDPVTAKDFVYAWQRLLDKNTAAEYAFIAYYIKNAEAINKGEKPLTDLGAKAVDDYTLEVELEKPVPYFLNLMAFPSYYPLNEKFVKEKGDKFGLEADTTLYNGPFVMASWKHEQGWQLKKNDKYWDNKTVKLEEINYSVVKEVATKVNLYDTGSIDFTLLSGEFVDKYKSNKEEYGEYSEASTFFLRLNQKRNGQDTPLKSKKLREAIALSIDKKGLATVILNNGSKATDQLVPKGLATGPDGKDYQDTFKNGLKYDPKKGAAAWEEAKKELGKDQVTIELLSYDDGTAKKIADYFKDQIEKNLKGVTVNTKIQPFKQKLKLESAQDYEVSFAGWSPDYSDPMTFIDMFESKSPYNQMSYSNPKYDEMVAKAGNELLSDPKKRWETLGKAEKLFLEEDAGLVPLYQTGRAYVMKPNVKGIVKHNISPEYSFKWAYVTEGK
GCTTGTTCTGGATCTGGTGGGGAGAAGAAATCAACTACGACGTCTAGTGGTGGTGGGGAAGAGAAAAAGTCTGAAATTAAATACGCAGCGAAACAAGTGTTAAATCGTACAGAGAATCAAGAGATTCCGACGATGGATGTTTCAAAATCTACCGATACATTAGGTTCTCAAATTTTAGGGAACACGATGGAAGGTTTATATCGATTAGATAAAGATAATAAGCCAATCCCAGCTGCAGCAGAATCTAGTACGAAAAGCGAGGATGGCAAAAAATATACATTTAAATTACGTAAAGATGCAAAATGGTCAAATGGTGATCCTGTAACAGCGAAAGATTTCGTATATGCATGGCAGCGCTTACTTGATAAAAATACAGCGGCAGAATATGCATTTATTGCTTACTATATTAAAAACGCAGAGGCAATTAATAAAGGTGAAAAACCACTAACAGATTTAGGAGCAAAAGCGGTAGATGATTATACGCTAGAAGTAGAATTAGAGAAACCAGTACCATATTTCTTGAATTTAATGGCATTCCCATCTTACTATCCTTTAAATGAAAAGTTCGTAAAAGAAAAAGGAGATAAATTCGGTTTAGAAGCAGATACAACGTTGTATAACGGACCGTTCGTTATGGCTTCATGGAAACATGAACAAGGATGGCAGCTAAAGAAAAATGATAAGTACTGGGATAATAAGACTGTAAAATTAGAAGAAATTAACTATAGTGTAGTAAAAGAAGTTGCGACGAAAGTAAACTTATATGATACAGGATCAATTGATTTCACGTTATTATCAGGAGAATTCGTTGATAAATATAAATCGAACAAAGAAGAGTACGGCGAGTATTCGGAAGCAAGTACATTCTTCTTACGTTTAAATCAAAAGCGTAACGGACAAGATACACCGTTAAAGAGCAAAAAACTTCGTGAAGCGATCGCATTATCAATTGATAAAAAAGGATTAGCAACCGTTATTTTAAATAACGGTTCAAAAGCAACAGATCAATTAGTACCAAAAGGGCTTGCGACAGGACCAGACGGTAAAGACTACCAAGATACGTTTAAAAATGGTCTAAAATATGATCCGAAAAAAGGTGCAGCAGCTTGGGAAGAAGCGAAAAAAGAACTTGGAAAAGATCAAGTGACAATTGAATTACTAAGCTATGATGATGGAACTGCGAAAAAAATTGCTGACTACTTTAAAGATCAAATTGAGAAAAACTTAAAAGGTGTAACGGTTAACACGAAAATTCAACCGTTCAAACAAAAACTAAAATTAGAGTCAGCACAAGATTATGAAGTTTCGTTTGCAGGTTGGAGTCCAGATTATTCGGATCCAATGACATTTATTGATATGTTTGAATCGAAGAGCCCATATAACCAAATGAGTTATTCGAATCCAAAATATGATGAAATGGTAGCGAAAGCAGGTAATGAATTACTGTCTGATCCGAAGAAGCGTTGGGAAACGTTAGGAAAAGCAGAGAAATTATTCCTTGAAGAAGATGCAGGATTAGTTCCTTTATATCAAACAGGAAGAGCGTATGTAATGAAACCGAATGTAAAAGGAATTGTGAAACATAACATTAGTCCAGAATATAGCTTTAAGTGGGCTTATGTAACGGAAGGTAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,310 total letters Query= IDP05180.103 Length=2040 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2949 0.0 1_04F_F7_IDP05180.103_v73F.seq 682 0.0 E N L Y F Q S N A A C S G S G G E K K S T T T S S G G G E E Query_1 175 GAGAACCTGTACTTCCAATCCAATGCCGCTTGTTCTGGATCTGGTGGGGAGAAGAAATCAACTACGACGTCTAGTGGTGGTGGGGAAGAG 264 0 1 ............................................................... 63 1 57 ..N......C......T..........N...C...............NT.N..A...........C.......N..T............. 146 K K S E I K Y A A K Q V L N R T E N Q E I P T M D V S K S T Query_1 265 AAAAAGTCTGAAATTAAATACGCAGCGAAACAAGTGTTAAATCGTACAGAGAATCAAGAGATTCCGACGATGGATGTTTCAAAATCTACC 354 0 64 .......................................................................................... 153 1 147 ......C....T...CT......T.............N..T..N............T......T...........-..........C... 235 D T L G S Q I L G N T M E G L Y R L D K D N K P I P A A A E Query_1 355 GATACATTAGGTTCTCAAATTTTAGGGAACACGATGGAAGGTTTATATCGATTAGATAAAGATAATAAGCCAATCCCAGCTGCAGCAGAA 444 0 154 .......................................................................................... 243 1 236 .........T.....TNC-....................T.........CC...T.......C......................NT... 324 S S T K S E D G K K Y T F K L R K D A K W S N G D P V T A K Query_1 445 TCTAGTACGAAAAGCGAGGATGGCAAAAAATATACATTTAAATTACGTAAAGATGCAAAATGGTCAAATGGTGATCCTGTAACAGCGAAA 534 0 244 .......................................................................................... 333 1 325 ...N......C.............T.............CC....N...T........NN......C.............C......N... 414 D F V Y A W Q R L L D K N T A A E Y A F I A Y Y I K N A E A Query_1 535 GATTTCGTATATGCATGGCAGCGCTTACTTGATAAAAATACAGCGGCAGAATATGCATTTATTGCTTACTATATTAAAAACGCAGAGGCA 624 0 334 .......................................................................................... 423 1 415 ......TC...........NN.A...................C..NTTC......T...CC....................N..A.TN.- 503 I N K G E K P L T D L G A K A V D D Y T L E V E L E K P V P Query_1 625 ATTAATAAAGGTGAAAAACCACTAACAGATTTAGGAGCAAAAGCGGTAGATGATTATACGCTAGAAGTAGAATTAGAGAAACCAGTACCA 714 0 424 .......................................................................................... 513 1 504 T..TT..N.TN.........NTCT.....-...N..-T......A...N..........N..T.N.N..N.....T.......N...... 591 Y F L N L M A F P S Y Y P L N E K F V K E K G D K F G L E Query_1 715 TATTTCTTGAATTTAATGGCATTCCCATCTTACTATCCTTTAAATGAAAAGTTCGTAAAAGAAA-AAGGAGATAAATTCGGTTTAGAAGC 803 0 514 ................................................................-......................... 602 1 592 .............N..............T...................N..C..T.....N...N.....T...... 668 A D T T L Y N G P F V M A S W K H E Q G W Q L K K N D K Y W Query_1 804 AGATACAACGTTGTATAACGGACCGTTCGTTATGGCTTCATGGAAACATGAACAAGGATGGCAGCTAAAGAAAAATGATAAGTACTGGGA 893 0 603 .......................................................................................... 692 0 618 .............. 605 D N K T V K L E E I N Y S V V K E V A T K V N L Y D T G S I Query_1 894 TAATAAGACTGTAAAATTAGAAGAAATTAACTATAGTGTAGTAAAAGAAGTTGCGACGAAAGTAAACTTATATGATACAGGATCAATTGA 983 0 693 .......................................................................................... 782 D F T L L S G E F V D K Y K S N K E E Y G E Y S E A S T F F Query_1 984 TTTCACGTTATTATCAGGAGAATTCGTTGATAAATATAAATCGAACAAAGAAGAGTACGGCGAGTATTCGGAAGCAAGTACATTCTTCTT 1073 0 783 .......................................................................................... 872 L R L N Q K R N G Q D T P L K S K K L R E A I A L S I D K K Query_1 1074 ACGTTTAAATCAAAAGCGTAACGGACAAGATACACCGTTAAAGAGCAAAAAACTTCGTGAAGCGATCGCATTATCAATTGATAAAAAAGG 1163 0 873 .......................................................................................... 962 0 956 .............. 943 G L A T V I L N N G S K A T D Q L V P K G L A T G P D G K D Query_1 1164 ATTAGCAACCGTTATTTTAAATAACGGTTCAAAAGCAACAGATCAATTAGTACCAAAAGGGCTTGCGACAGGACCAGACGGTAAAGACTA 1253 0 963 .......................................................................................... 1052 0 991 ...........A........... 969 Y Q D T F K N G L K Y D P K K G A A A W E E A K K E L G K D Query_1 1254 CCAAGATACGTTTAAAAATGGTCTAAAATATGATCCGAAAAAAGGTGCAGCAGCTTGGGAAGAAGCGAAAAAAGAACTTGGAAAAGATCA 1343 0 1053 .......................................................................................... 1142 Q V T I E L L S Y D D G T A K K I A D Y F K D Q I E K N L K Query_1 1344 AGTGACAATTGAATTACTAAGCTATGATGATGGAACTGCGaaaaaaaTTGCTGACTACTTTAAAGATCAAATTGAGAAAAACTTAAAAGG 1433 0 1143 .......................................................................................... 1232 G V T V N T K I Q P F K Q K L K L E S A Q D Y E V S F A G W Query_1 1434 TGTAACGGTTAACACGAAAATTCAACCGTTCAAACAAAAACTAAAATTAGAGTCAGCACAAGATTATGAAGTTTCGTTTGCAGGTTGGAG 1523 0 1233 .......................................................................................... 1322 S P D Y S D P M T F I D M F E S K S P Y N Q M S Y S N P K Y Query_1 1524 TCCAGATTATTCGGATCCAATGACATTTATTGATATGTTTGAATCGAAGAGCCCATATAACCAAATGAGTTATTCGAATCCAAAATATGA 1613 0 1323 .......................................................................................... 1412 D E M V A K A G N E L L S D P K K R W E T L G K A E K L F L Query_1 1614 TGAAATGGTAGCGAAAGCAGGTAATGAATTACTGTCTGATCCGAAGAAGCGTTGGGAAACGTTAGGAAAAGCAGAGAAATTATTCCTTGA 1703 0 1413 .......................................................................................... 1502 E E D A G L V P L Y Q T G R A Y V M K P N V K G I V K H N I Query_1 1704 AGAAGATGCAGGATTAGTTCCTTTATATCAAACAGGAAGAGCGTATGTAATGAAACCGAATGTAAAAGGAATTGTGAAACATAACATTAG 1793 0 1503 .......................................................................................... 1592 S P E Y S F K W A Y V T E G K Query_1 1794 TCCAGAATATAGCTTTAAGTGGGCTTATGTAACGGAAGGTAAA 1836 0 1593 ........................................... 1635 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4578288 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 2,310 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP05387.102 (IDP05387.21-553.P(553)..pMCSG68)
GCGNGEKASTTKKEAGKGAADKQVLNLLETAEIPSMDTSKSTDSVSFRAFVNAMEGLYRLDKDNKPTPGMAKDVKISEDKKIYTFELRDAKWSNGDPVRAQDFVYAWQRTLDKATAAEYAFILFDVKNAQKVNKGELPLDQLGVKAKDDKTLVVELEQPAPYFLELTSFPTFFPLNEKYVKEQGDKYALEADKLLYNGPFVMSEWKHERSYQLKKNPNYWDKDTVKLEEINVNIVKETSTGVNLYDSDQADRVILSSEFVDKYKKNKPDEFKTKLDPRMYFLRFNQKNATFKNQKVREAIDLAYDKKGIANVILNDGSLPAYFLVPEKFTTGPDGKDFRSVNKNFRDSKDNAAKAKKLWEEAKKELGQDTITVELLNDDNGSRKKVGDFIKEELEKNLPGIKVNLKQQPYKQKLDLEKKFEYEFSLSAWSPDYPDPMTYIDMFVTGSSFNEMDYSNPKYDELVAKSKGELLKDDKARWNALAEAEKILIGQDAAISPTYQQSTAYLEKPYVKGIANHTFGGDFSYKWAYITKK
GGTTGCGGAAATGGGGAGAAGGCATCTACAACGAAGAAAGAAGCAGGTAAGGGAGCAGCGGATAAGCAAGTATTAAACTTACTAGAAACAGCAGAAATTCCTTCAATGGATACGTCAAAATCAACAGACTCTGTATCATTCCGTGCCTTTGTAAATGCAATGGAAGGATTATATCGTTTAGATAAGGATAATAAACCAACTCCAGGTATGGCAAAAGATGTGAAAATTAGTGAAGATAAAAAAATATATACATTTGAATTACGAGACGCAAAGTGGTCTAATGGCGATCCAGTTCGAGCGCAAGATTTTGTATATGCATGGCAACGTACATTAGATAAAGCAACGGCAGCTGAGTATGCATTTATTTTATTTGATGTAAAGAATGCACAGAAAGTCAATAAAGGAGAATTACCGTTAGATCAATTAGGGGTGAAAGCGAAGGATGATAAAACATTAGTAGTAGAATTAGAACAACCAGCACCATATTTCCTTGAGCTTACTTCATTCCCAACATTTTTCCCTTTAAATGAAAAATATGTGAAAGAACAAGGGGATAAATATGCATTAGAAGCAGATAAACTATTATACAATGGCCCATTTGTAATGAGTGAATGGAAACATGAACGAAGTTATCAGTTAAAGAAAAATCCTAATTATTGGGATAAAGATACTGTGAAATTAGAAGAAATTAATGTGAATATTGTAAAGGAAACGAGCACGGGAGTAAATTTATATGATAGTGATCAAGCAGACCGAGTTATTTTAAGTTCTGAATTTGTTGATAAATATAAAAAGAATAAACCAGATGAATTTAAAACGAAGTTAGATCCGCGTATGTATTTCTTACGCTTTAACCAGAAGAATGCAACGTTTAAAAACCAAAAAGTTCGTGAAGCGATTGATCTGGCATATGATAAAAAAGGAATTGCAAATGTTATTTTAAATGATGGATCATTGCCAGCATACTTCCTAGTACCTGAAAAATTTACGACAGGACCAGATGGGAAAGATTTCCGTTCTGTAAATAAAAATTTCCGTGACAGTAAAGATAATGCAGCAAAAGCAAAAAAACTATGGGAAGAAGCGAAGAAAGAACTTGGTCAAGATACAATCACAGTAGAATTACTAAATGATGACAACGGTAGTCGGAAAAAAGTAGGAGACTTTATTAAAGAAGAGTTAGAGAAAAACTTACCTGGTATTAAAGTGAATTTAAAACAACAGCCATATAAGCAAAAGTTAGATTTAGAAAAGAAATTTGAATATGAATTCTCTTTATCTGCATGGAGTCCAGATTATCCAGATCCAATGACTTATATTGATATGTTCGTTACGGGAAGCTCATTTAATGAAATGGATTATTCTAATCCGAAGTATGATGAGTTAGTAGCGAAATCAAAAGGAGAGCTATTAAAAGATGATAAAGCACGCTGGAATGCACTAGCAGAAGCTGAAAAAATCTTAATTGGTCAAGATGCTGCCATTTCTCCTACGTACCAACAAAGTACCGCATACTTAGAGAAACCATACGTAAAAGGTATTGCAAATCATACGTTTGGTGGGGACTTCAGTTATAAATGGGCATATATTACAAAAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 1,609 total letters Query= IDP05387.102 Length=2004 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2884 0.0 H S S G V D L W S H P Q F E K G T E N L Y F Q S N A G C G N Query_1 124 CATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGGTTGCGGAAAT 213 0 1 ............ 12 0 865 ............ 854 G E K A S T T K K E A G K G A A D K Q V L N L L E T A E I P Query_1 214 GGGGAGAAGGCATCTACAACGAAGAAAGAAGCAGGTAAGGGAGCAGCGGATAAGCAAGTATTAAACTTACTAGAAACAGCAGAAATTCCT 303 0 13 .......................................................................................... 102 0 1085 ........... 1095 S M D T S K S T D S V S F R A F V N A M E G L Y R L D K D N Query_1 304 TCAATGGATACGTCAAAATCAACAGACTCTGTATCATTCCGTGCCTTTGTAAATGCAATGGAAGGATTATATCGTTTAGATAAGGATAAT 393 0 103 .......................................................................................... 192 K P T P G M A K D V K I S E D K K I Y T F E L R D A K W S N Query_1 394 AAACCAACTCCAGGTATGGCAAAAGATGTGAAAATTAGTGAAGATaaaaaaaTATATACATTTGAATTACGAGACGCAAAGTGGTCTAAT 483 0 193 .......................................................................................... 282 G D P V R A Q D F V Y A W Q R T L D K A T A A E Y A F I L F Query_1 484 GGCGATCCAGTTCGAGCGCAAGATTTTGTATATGCATGGCAACGTACATTAGATAAAGCAACGGCAGCTGAGTATGCATTTATTTTATTT 573 0 283 .......................................................................................... 372 D V K N A Q K V N K G E L P L D Q L G V K A K D D K T L V V Query_1 574 GATGTAAAGAATGCACAGAAAGTCAATAAAGGAGAATTACCGTTAGATCAATTAGGGGTGAAAGCGAAGGATGATAAAACATTAGTAGTA 663 0 373 .......................................................................................... 462 E L E Q P A P Y F L E L T S F P T F F P L N E K Y V K E Q G Query_1 664 GAATTAGAACAACCAGCACCATATTTCCTTGAGCTTACTTCATTCCCAACATTTTTCCCTTTAAATGAAAAATATGTGAAAGAACAAGGG 753 0 463 .......................................................................................... 552 D K Y A L E A D K L L Y N G P F V M S E W K H E R S Y Q L K Query_1 754 GATAAATATGCATTAGAAGCAGATAAACTATTATACAATGGCCCATTTGTAATGAGTGAATGGAAACATGAACGAAGTTATCAGTTAAAG 843 0 553 .......................................................................................... 642 K N P N Y W D K D T V K L E E I N V N I V K E T S T G V N L Query_1 844 AAAAATCCTAATTATTGGGATAAAGATACTGTGAAATTAGAAGAAATTAATGTGAATATTGTAAAGGAAACGAGCACGGGAGTAAATTTA 933 0 643 .......................................................................................... 732 Y D S D Q A D R V I L S S E F V D K Y K K N K P D E F K T K Query_1 934 TATGATAGTGATCAAGCAGACCGAGTTATTTTAAGTTCTGAATTTGTTGATAAATATAAAAAGAATAAACCAGATGAATTTAAAACGAAG 1023 0 733 .......................................................................................... 822 0 1208 ............ 1219 L D P R M Y F L R F N Q K N A T F K N Q K V R E A I D L A Y Query_1 1024 TTAGATCCGCGTATGTATTTCTTACGCTTTAACCAGAAGAATGCAACGTTTAAAAACCAAAAAGTTCGTGAAGCGATTGATCTGGCATAT 1113 0 823 .......................................................................................... 912 D K K G I A N V I L N D G S L P A Y F L V P E K F T T G P D Query_1 1114 GATAAAAAAGGAATTGCAAATGTTATTTTAAATGATGGATCATTGCCAGCATACTTCCTAGTACCTGAAAAATTTACGACAGGACCAGAT 1203 0 913 .......................................................................................... 1002 G K D F R S V N K N F R D S K D N A A K A K K L W E E A K K Query_1 1204 GGGAAAGATTTCCGTTCTGTAAATAAAAATTTCCGTGACAGTAAAGATAATGCAGCAAAAGCaaaaaaaCTATGGGAAGAAGCGAAGAAA 1293 0 1003 .......................................................................................... 1092 0 32 ........ 39 E L G Q D T I T V E L L N D D N G S R K K V G D F I K E E L Query_1 1294 GAACTTGGTCAAGATACAATCACAGTAGAATTACTAAATGATGACAACGGTAGTCGGAAAAAAGTAGGAGACTTTATTAAAGAAGAGTTA 1383 0 1093 .......................................................................................... 1182 0 40 ... 42 0 1466 ........... 1476 E K N L P G I K V N L K Q Q P Y K Q K L D L E K K F E Y E F Query_1 1384 GAGAAAAACTTACCTGGTATTAAAGTGAATTTAAAACAACAGCCATATAAGCAAAAGTTAGATTTAGAAAAGAAATTTGAATATGAATTC 1473 0 1183 .......................................................................................... 1272 0 807 ............ 818 S L S A W S P D Y P D P M T Y I D M F V T G S S F N E M D Y Query_1 1474 TCTTTATCTGCATGGAGTCCAGATTATCCAGATCCAATGACTTATATTGATATGTTCGTTACGGGAAGCTCATTTAATGAAATGGATTAT 1563 0 1273 .......................................................................................... 1362 S N P K Y D E L V A K S K G E L L K D D K A R W N A L A E A Query_1 1564 TCTAATCCGAAGTATGATGAGTTAGTAGCGAAATCAAAAGGAGAGCTATTAAAAGATGATAAAGCACGCTGGAATGCACTAGCAGAAGCT 1653 0 1363 .......................................................................................... 1452 E K I L I G Q D A A I S P T Y Q Q S T A Y L E K P Y V K G I Query_1 1654 GAAAAAATCTTAATTGGTCAAGATGCTGCCATTTCTCCTACGTACCAACAAAGTACCGCATACTTAGAGAAACCATACGTAAAAGGTATT 1743 0 1453 .......................................................................................... 1542 0 1097 ........... 1107 A N H T F G G D F S Y K W A Y I T K K Query_1 1744 GCAAATCATACGTTTGGTGGGGACTTCAGTTATAAATGGGCATATATTACaaaaaaa 1800 0 1543 ......................................................... 1599 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3110796 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 1,609 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP05634.105 (IDP05634.31-270.P(270)..pMCSG73)
KEEVKNEKVITVASSNSADPYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMSKERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVATSVNKDNLFQEKVYGDGTSGLQDLDLGRVSAWIDAELTIVGDAKKANMKVRVLDKKLSATNIAYFFKKNDDKSKTIKEDVNKAIEEMLADGTVKKISEKWFGMDVSADIQK
AAAGAGGAAGTAAAGAATGAAAAAGTTATAACTGTAGCTTCATCTAATTCAGCTGACCCATATTCTTATGTTGATAATGGTGAGCATAAGGGTTTTGAAGTTGACATGTGGAAAGAGATTGGTAAAAGAAGTGGATATAAGATAAAAATGGAACCAACAGGTTTTAGTTCTATATTTGGTATGTTAGATTCTGGTAAAGTTGATGTGGCAGCCAACTTCTTTGGAATGTCTAAAGAAAGATTAGAAAAATACGATGCTTCTATACCTTATGGTTCTGATTCTGTGTGTATAGCTGTAAAAGATGGAAATAACGAAATTAATAAGCTTGAAGATTTAAAGGGAAAAGTTGTAGCAGTTTCAGAAGGTTCTCAAGGTCAACAGGTTGCAACTAGTGTTAATAAAGATAATTTGTTTCAAGAAAAGGTTTACGGTGATGGAACTAGTGGTCTTCAAGATTTAGATTTGGGAAGAGTTAGTGCATGGATTGATGCAGAGCTTACTATTGTGGGAGACGCTAAAAAAGCTAATATGAAAGTAAGAGTATTAGATAAAAAATTATCAGCTACTAATATAGCATACTTCTTTAAGAAAAACGATGATAAATCTAAAACGATAAAAGAAGATGTAAACAAAGCAATAGAAGAAATGCTTGCAGATGGAACAGTGAAAAAAATATCTGAAAAATGGTTTGGAATGGATGTAAGTGCTGATATACAAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 730 total letters Query= IDP05634.105 Length=1125 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1299 0.0 E I N T T H Y R G I V S G * Q F P S R N N F V * L * E G D Query_1 12 CGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATAT 101 0 312 ........... 322 I H M H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Query_1 102 ACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCA 191 Q S N A K E E V K N E K V I T V A S S N S A D P Y S Y V D N Query_1 192 ATCCAATGCCAAAGAGGAAGTAAAGAATGAAAAAGTTATAACTGTAGCTTCATCTAATTCAGCTGACCCATATTCTTATGTTGATAATGG 281 0 1 ................................................................................ 80 G E H K G F E V D M W K E I G K R S G Y K I K M E P T G F S Query_1 282 TGAGCATAAGGGTTTTGAAGTTGACATGTGGAAAGAGATTGGTAAAAGAAGTGGATATAAGATAAAAATGGAACCAACAGGTTTTAGTTC 371 0 81 .......................................................................................... 170 S I F G M L D S G K V D V A A N F F G M S K E R L E K Y D A Query_1 372 TATATTTGGTATGTTAGATTCTGGTAAAGTTGATGTGGCAGCCAACTTCTTTGGAATGTCTAAAGAAAGATTAGAAAAATACGATGCTTC 461 0 171 .......................................................................................... 260 S I P Y G S D S V C I A V K D G N N E I N K L E D L K G K V Query_1 462 TATACCTTATGGTTCTGATTCTGTGTGTATAGCTGTAAAAGATGGAAATAACGAAATTAATAAGCTTGAAGATTTAAAGGGAAAAGTTGT 551 0 261 .......................................................................................... 350 V A V S E G S Q G Q Q V A T S V N K D N L F Q E K V Y G D G Query_1 552 AGCAGTTTCAGAAGGTTCTCAAGGTCAACAGGTTGCAACTAGTGTTAATAAAGATAATTTGTTTCAAGAAAAGGTTTACGGTGATGGAAC 641 0 351 .......................................................................................... 440 T S G L Q D L D L G R V S A W I D A E L T I V G D A K K A N Query_1 642 TAGTGGTCTTCAAGATTTAGATTTGGGAAGAGTTAGTGCATGGATTGATGCAGAGCTTACTATTGTGGGAGACGCTAAAAAAGCTAATAT 731 0 441 .......................................................................................... 530 M K V R V L D K K L S A T N I A Y F F K K N D D K S K T I K Query_1 732 GAAAGTAAGAGTATTAGATAAAAAATTATCAGCTACTAATATAGCATACTTCTTTAAGAAAAACGATGATAAATCTAAAACGATAAAAGA 821 0 531 .......................................................................................... 620 E D V N K A I E E M L A D G T V K K I S E K W F G M D V S A Query_1 822 AGATGTAAACAAAGCAATAGAAGAAATGCTTGCAGATGGAACAGTGaaaaaaaTATCTGAAAAATGGTTTGGAATGGATGTAAGTGCTGA 911 0 621 .......................................................................................... 710 D I Q K Query_1 912 TATACAAAAA 921 0 711 .......... 720 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 764368 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 730 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP09018.106 (IDP09018.1-369.F..pMCSG68)
MSQQVTQLNPTQQTTQSAFLATTVITAQCHAILNTQFTPPTVKPDWFDDLSKKLDSAKLVAKQWIDDLGPQVSASIPSSVINFDATFQASIDAIHELYKADPTASGKDNTTVQQASQIMTALSSQVSGIEATVKGMNKELSDWGVKMQAAHDDLVNGATNIQKTIIDLQTDIESMNNAIDNNRAAIEKLNKDLVYAQVAVGVGIFMLVAGVALTVATAGTAAAVSGGIAAVGAASIIAGGVTWGVLQNQIDDDYDSIAQEQKQKAEDQQQIIALQGLSNASSAVVSAIETSTSVLSDFETTWTVFGNELDDVVTKLNNGASMQSIIMEKVMSDAAKNEWDDAVELAKQLASAKIAIETKELAPAVKQAA
ATGTCACAACAAGTTACTCAGTTGAACCCAACACAGCAAACCACTCAATCTGCATTTTTGGCAACCACAGTCATCACAGCGCAGTGCCATGCCATTTTAAACACACAATTTACGCCACCTACGGTAAAACCAGATTGGTTTGATGACCTGAGTAAAAAGTTAGACTCAGCAAAATTGGTTGCTAAGCAATGGATTGACGATTTGGGACCACAAGTATCAGCATCGATTCCCTCCAGCGTAATTAATTTTGACGCTACTTTTCAAGCATCAATCGATGCCATTCATGAGTTATATAAAGCGGATCCAACAGCAAGTGGCAAAGACAATACAACGGTTCAGCAAGCTTCACAGATTATGACCGCCCTATCTTCTCAAGTCAGCGGTATCGAAGCCACAGTCAAAGGGATGAATAAGGAACTCTCTGATTGGGGGGTGAAAATGCAAGCAGCACATGATGACTTAGTCAATGGTGCGACCAATATTCAAAAAACAATTATTGATTTACAAACAGATATTGAAAGTATGAATAACGCAATAGACAACAATCGTGCAGCCATTGAGAAACTGAATAAAGATCTGGTCTATGCACAAGTTGCGGTTGGTGTTGGCATCTTTATGCTCGTCGCAGGCGTTGCGCTTACCGTCGCAACCGCTGGCACCGCAGCTGCAGTCTCCGGCGGTATCGCAGCCGTCGGCGCGGCATCGATTATTGCTGGTGGCGTTACATGGGGCGTATTACAAAATCAAATTGATGATGACTACGACAGCATCGCGCAAGAACAAAAGCAAAAAGCTGAAGATCAACAACAGATTATCGCTTTACAGGGGCTTTCCAATGCCAGTAGCGCCGTTGTTTCAGCGATTGAAACATCGACATCAGTATTATCTGATTTTGAAACAACGTGGACTGTCTTTGGCAACGAGTTAGATGATGTAGTAACAAAGCTTAATAATGGTGCATCCATGCAGAGCATTATAATGGAAAAAGTGATGTCTGACGCAGCGAAAAACGAATGGGATGATGCGGTTGAACTTGCTAAACAGCTAGCTAGCGCAAAAATAGCTATCGAAACGAAAGAGTTAGCACCTGCAGTAAAACAAGCAGCT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 1,432 total letters Query= IDP09018.106 Length=1512 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1997 0.0 2_08E_E2_IDP09018.106_T7term.seq 506 8e-147 M S Q Q V T Q L N P T Q Q T T Q S A F L A T T V I T A Q C H Query_1 202 ATGTCACAACAAGTTACTCAGTTGAACCCAACACAGCAAACCACTCAATCTGCATTTTTGGCAACCACAGTCATCACAGCGCAGTGCCAT 291 0 1 .......................................................................................... 90 A I L N T Q F T P P T V K P D W F D D L S K K L D S A K L V Query_1 292 GCCATTTTAAACACACAATTTACGCCACCTACGGTAAAACCAGATTGGTTTGATGACCTGAGTAAAAAGTTAGACTCAGCAAAATTGGTT 381 0 91 .......................................................................................... 180 A K Q W I D D L G P Q V S A S I P S S V I N F D A T F Q A S Query_1 382 GCTAAGCAATGGATTGACGATTTGGGACCACAAGTATCAGCATCGATTCCCTCCAGCGTAATTAATTTTGACGCTACTTTTCAAGCATCA 471 0 181 .......................................................................................... 270 I D A I H E L Y K A D P T A S G K D N T T V Q Q A S Q I M T Query_1 472 ATCGATGCCATTCATGAGTTATATAAAGCGGATCCAACAGCAAGTGGCAAAGACAATACAACGGTTCAGCAAGCTTCACAGATTATGACC 561 0 271 .......................................................................................... 360 A L S S Q V S G I E A T V K G M N K E L S D W G V K M Q A A Query_1 562 GCCCTATCTTCTCAAGTCAGCGGTATCGAAGCCACAGTCAAAGGGATGAATAAGGAACTCTCTGATTGGGGGGTGAAAATGCAAGCAGCA 651 0 361 .......................................................................................... 450 H D D L V N G A T N I Q K T I I D L Q T D I E S M N N A I D Query_1 652 CATGATGACTTAGTCAATGGTGCGACCAATATTCAAAAAACAATTATTGATTTACAAACAGATATTGAAAGTATGAATAACGCAATAGAC 741 0 451 .......................................................................................... 540 N N R A A I E K L N K D L V Y A Q V A V G V G I F M L V A G Query_1 742 AACAATCGTGCAGCCATTGAGAAACTGAATAAAGATCTGGTCTATGCACAAGTTGCGGTTGGTGTTGGCATCTTTATGCTCGTCGCAGGC 831 0 541 .......................................................................................... 630 V A L T V A T A G T A A A V S G G I A A V G A A S I I A G G Query_1 832 GTTGCGCTTACCGTCGCAACCGCTGGCACCGCAGCTGCAGTCTCCGGCGGTATCGCAGCCGTCGGCGCGGCATCGATTATTGCTGGTGGC 921 0 631 .......................................................................................... 720 V T W G V L Q N Q I D D D Y D S I A Q E Q K Q K A E D Q Q Q Query_1 922 GTTACATGGGGCGTATTACAAAATCAAATTGATGATGACTACGACAGCATCGCGCAAGAACAAAAGCAAAAAGCTGAAGATCAACAACAG 1011 0 721 .......................................................................................... 810 I I A L Q G L S N A S S A V V S A I E T S T S V L S D F E T Query_1 1012 ATTATCGCTTTACAGGGGCTTTCCAATGCCAGTAGCGCCGTTGTTTCAGCGATTGAAACATCGACATCAGTATTATCTGATTTTGAAACA 1101 0 811 .......................................................................................... 900 2 320 .... 317 T W T V F G N E L D D V V T K L N N G A S M Q S I I M E K V Query_1 1102 ACGTGGACTGTCTTTGGCAACGAGTTAGATGATGTAGTAACAAAGCTTAATAATGGTGCATCCATGCAGAGCATTATAATGGAAAAAGTG 1191 0 901 .......................................................................................... 990 2 316 ...................N...................................................................... 227 M S D A A K N E W D D A V E L A K Q L A S A K I A I E T K E Query_1 1192 ATGTCTGACGCAGCGAAAAACGAATGGGATGATGCGGTTGAACTTGCTAAACAGCTAGCTAGCGCAAAAATAGCTATCGAAACGAAAGAG 1281 0 991 .......................................................................................... 1080 2 226 ........................................................................N................. 137 L A P A V K Q A A * H W K W I T D P N S S S V D K L A A A L Query_1 1282 TTAGCACCTGCAGTAAAACAAGCAGCTTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTC 1371 0 1081 ........................... 1107 2 136 .............................................................................N.........N.. 47 E H H H H H H * D P Query_1 1372 GAGcaccaccaccaccaccacTGAG-ATCCGG 1402 2 46 --.G.G..............N....A...... 17 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2076339 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 1,432 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP90822.103 (IDP90822.1-321.F..pMCSG73)
MHVLIVKTSSMGDVLHTLPALTDAMNAIPGIRFDWVVEEGFSQIPSWHPAVDKVIPVAIRRWRKNWFGSDTRQERCDFKRVVQQRSYDRVIDAQGLIKSAALITRIAKGTKHGPDCKSAREPFASWFYNCRHEIDTKQHAVERIRQLFAKSLGYDKPESYGDYAIAQRFLNQLPADAGQYLVFLHATTRDNKHWPESHWVQLIELVQPTGLKIKLPWGAEHEHQRALRLAEHFPHVEVLPKLSLQQVAEVLAGAKAVVSVDTGLSHLTAALDRPNITLFGPTDPGLIGGYGKNQIAVISEQKKMDSLSAPTIMARLETLIS
ATGCACGTATTAATCGTTAAAACCTCGTCAATGGGCGATGTTTTACATACCTTACCCGCGCTGACGGATGCCATGAATGCCATACCCGGTATTCGCTTCGATTGGGTGGTAGAAGAAGGGTTCAGCCAAATCCCCAGTTGGCATCCGGCGGTGGATAAAGTGATCCCGGTGGCCATCCGCCGTTGGCGTAAAAACTGGTTTGGTAGTGATACCCGGCAGGAACGCTGTGATTTTAAGCGTGTGGTACAACAACGCAGTTATGATCGGGTCATTGATGCCCAAGGGTTGATTAAAAGCGCCGCGCTGATAACCCGGATTGCGAAAGGCACGAAGCACGGGCCGGATTGTAAAAGTGCCCGTGAGCCGTTTGCCAGTTGGTTTTATAACTGCCGCCATGAAATTGATACCAAACAACATGCCGTAGAACGTATACGTCAACTGTTTGCTAAAAGCCTCGGCTATGATAAACCGGAAAGTTATGGCGACTATGCCATCGCACAACGCTTTTTAAATCAGTTACCGGCCGATGCAGGTCAGTACTTGGTTTTCCTACATGCGACGACCCGTGACAACAAGCACTGGCCCGAGAGCCATTGGGTACAATTGATTGAGTTAGTGCAACCCACAGGTTTAAAAATCAAACTACCGTGGGGCGCAGAACATGAGCATCAGCGCGCTTTACGATTAGCGGAACATTTCCCACACGTAGAGGTTCTGCCTAAGCTCAGTCTTCAGCAGGTCGCCGAGGTTCTGGCGGGTGCAAAAGCCGTAGTCTCTGTCGATACTGGCCTCAGCCATCTGACCGCCGCCCTGGATCGCCCAAACATTACGCTGTTTGGCCCCACTGACCCCGGTTTAATCGGCGGCTACGGGAAAAACCAAATTGCCGTGATTTCCGAGCAAAAAAAGATGGATAGCCTCTCTGCGCCCACTATTATGGCACGATTAGAAACACTCATTTCA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 1,129 total letters Query= IDP90822.103 Length=1368 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1737 0.0 M H V L I V K T S S M G D V L H T L P A L T D A M N A I P G Query_1 202 ATGCACGTATTAATCGTTAAAACCTCGTCAATGGGCGATGTTTTACATACCTTACCCGCGCTGACGGATGCCATGAATGCCATACCCGGT 291 0 1 .......................................................................................... 90 I R F D W V V E E G F S Q I P S W H P A V D K V I P V A I R Query_1 292 ATTCGCTTCGATTGGGTGGTAGAAGAAGGGTTCAGCCAAATCCCCAGTTGGCATCCGGCGGTGGATAAAGTGATCCCGGTGGCCATCCGC 381 0 91 .......................................................................................... 180 R W R K N W F G S D T R Q E R C D F K R V V Q Q R S Y D R V Query_1 382 CGTTGGCGTAAAAACTGGTTTGGTAGTGATACCCGGCAGGAACGCTGTGATTTTAAGCGTGTGGTACAACAACGCAGTTATGATCGGGTC 471 0 181 .......................................................................................... 270 I D A Q G L I K S A A L I T R I A K G T K H G P D C K S A R Query_1 472 ATTGATGCCCAAGGGTTGATTAAAAGCGCCGCGCTGATAACCCGGATTGCGAAAGGCACGAAGCACGGGCCGGATTGTAAAAGTGCCCGT 561 0 271 .......................................................................................... 360 E P F A S W F Y N C R H E I D T K Q H A V E R I R Q L F A K Query_1 562 GAGCCGTTTGCCAGTTGGTTTTATAACTGCCGCCATGAAATTGATACCAAACAACATGCCGTAGAACGTATACGTCAACTGTTTGCTAAA 651 0 361 .......................................................................................... 450 S L G Y D K P E S Y G D Y A I A Q R F L N Q L P A D A G Q Y Query_1 652 AGCCTCGGCTATGATAAACCGGAAAGTTATGGCGACTATGCCATCGCACAACGCTTTTTAAATCAGTTACCGGCCGATGCAGGTCAGTAC 741 0 451 .......................................................................................... 540 L V F L H A T T R D N K H W P E S H W V Q L I E L V Q P T G Query_1 742 TTGGTTTTCCTACATGCGACGACCCGTGACAACAAGCACTGGCCCGAGAGCCATTGGGTACAATTGATTGAGTTAGTGCAACCCACAGGT 831 0 541 .......................................................................................... 630 L K I K L P W G A E H E H Q R A L R L A E H F P H V E V L P Query_1 832 TTAAAAATCAAACTACCGTGGGGCGCAGAACATGAGCATCAGCGCGCTTTACGATTAGCGGAACATTTCCCACACGTAGAGGTTCTGCCT 921 0 631 .......................................................................................... 720 K L S L Q Q V A E V L A G A K A V V S V D T G L S H L T A A Query_1 922 AAGCTCAGTCTTCAGCAGGTCGCCGAGGTTCTGGCGGGTGCAAAAGCCGTAGTCTCTGTCGATACTGGCCTCAGCCATCTGACCGCCGCC 1011 0 721 .......................................................................................... 810 L D R P N I T L F G P T D P G L I G G Y G K N Q I A V I S E Query_1 1012 CTGGATCGCCCAAACATTACGCTGTTTGGCCCCACTGACCCCGGTTTAATCGGCGGCTACGGGAAAAACCAAATTGCCGTGATTTCCGAG 1101 0 811 .......................................................................................... 900 Q K K M D S L S A P T I M A R L E T L I S Query_1 1102 CaaaaaaaGATGGATAGCCTCTCTGCGCCCACTATTATGGCACGATTAGAAACACTCATTTCA 1164 0 901 ............................................................... 963 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1466652 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 1,129 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP91062.103 (IDP91062.26-266.P(266)..pMCSG73)
ADATSPVVTNSAFKNNDQQSAYALGASLGRYMDNSLKEQEKLGIKLDKDQLIAGVQDAFANKSKLTDEEIEKTLQNFEARVKASAQAKMEQDAKENADKGAKYRDTFAKEKGVKKTASGLLYKVENAGTGDAPKDSDTVVVNYKGTLADGTEFDNSYKRGEPLSFRLDGVIPGWTEGLKQIKKGGKITLVIPPELAYGKAGVPGIPANSTLIFDVELLDVKAAAKADAQEQQPAVDTKAKK
GCTGACGCAACCTCACCAGTAGTAACCAATAGCGCATTTAAAAATAATGATCAGCAATCTGCTTATGCATTAGGTGCGTCTTTGGGCCGCTATATGGATAATTCCCTGAAAGAACAAGAAAAATTGGGGATTAAGTTGGATAAAGACCAGTTGATCGCTGGTGTGCAAGATGCTTTTGCTAACAAAAGCAAACTGACTGACGAAGAGATCGAAAAAACCCTGCAAAATTTTGAAGCTCGGGTAAAAGCGTCTGCTCAAGCCAAAATGGAGCAAGATGCCAAAGAAAACGCGGATAAAGGCGCTAAGTACCGCGATACCTTTGCTAAAGAAAAAGGTGTGAAGAAAACCGCATCTGGCCTGCTGTACAAGGTAGAGAACGCCGGTACGGGTGATGCACCAAAAGACAGCGATACTGTTGTCGTTAACTACAAAGGCACTTTGGCTGACGGAACTGAGTTTGATAACTCATACAAACGTGGCGAGCCATTGTCTTTCCGTCTGGATGGCGTTATCCCAGGCTGGACTGAAGGCCTGAAGCAAATCAAAAAAGGTGGCAAAATCACCTTGGTTATCCCGCCAGAACTGGCTTACGGCAAAGCAGGCGTCCCTGGTATTCCTGCCAACTCTACTCTGATATTCGATGTAGAACTGCTGGATGTGAAAGCCGCAGCTAAAGCCGATGCTCAAGAGCAACAGCCTGCGGTTGATACAAAAGCTAAGAAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,242 total letters Query= IDP91062.103 Length=1128 Score E Sequences producing significant alignments: (Bits) Value 2_07B_B8_IDP91062.103_T7term.seq 1389 0.0 0_wt 1305 0.0 1_05B_B8_IDP91062.103_v73F.seq 820 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCA-GTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAA 197 2 844 ...........A..A......G....------...................... 797 2 862 .....C........... 846 1 43 ...........A..-......G....------...............T...... 89 N A A D A T S P V V T N S A F K N N D Q Q S A Y A L G A S L Query_1 198 TGCCGCTGACGCAACCTCACCAGTAGTAA-CCAATAGCGCATTTAAAAATAATGATCAGCAATCTGCTTATGCATTAGGTGCGTCTTTGG 286 2 796 .............................A............................................................ 707 0 1 .........................-............................................................ 85 1 90 ..................N....N.....-............................................................ 178 G R Y M D N S L K E Q E K L G I K L D K D Q L I A G V Q D A Query_1 287 GCCGCTATATGGATAATTCCCTGAAAGAACAAGAAAAATTGGGGATTAAGTTGGATAAAGACCAGTTGATCGCTGGTGTGCAAGATGCTT 376 2 706 .......................................................................................... 617 0 86 .......................................................................................... 175 1 179 ............................N............................................................. 268 F A N K S K L T D E E I E K T L Q N F E A R V K A S A Q A K Query_1 377 TTGCTAACAAAAGCAAACTGACTGACGAA-GAGATCGAAAAAACCCTGCAAAATTTTGAAGCTCGGGTAAAAGCGTCTGCTCAAGCCAAA 465 2 616 .............................A............................................................ 527 2 39 ........... 29 0 176 .............................-............................................................ 264 1 269 ..........................N..-N.....N..................................................... 357 M E Q D A K E N A D K G A K Y R D T F A K E K G V K K T A S Query_1 466 ATGGAGCAAGATGCCAAAGAAAACGCGGATAAAGGCGCTAAGTACCGCGATACCTTTGCTAAAGAAAAAGGTGTGAAGAAAACCGCATCT 555 2 526 .......................................................................................... 437 0 265 .......................................................................................... 354 1 358 .......................................................................................... 447 G L L Y K V E N A G T G D A P K D S D T V V V N Y K G T L A Query_1 556 GGCCTGCTGTACAAGGTAGAGAACGCCGGTACGGGTGATGCACCAAAAGACAGCGATACTGTTGTCGTTAACTACAAAGGCACTTTGGCT 645 2 436 .......................................................................................... 347 0 355 .......................................................................................... 444 1 448 ...........................................................................N... 526 D G T E F D N S Y K R G E P L S F R L D G V I P G W T E G L Query_1 646 GACGGAACTGAGTTTGATAACTCATACAAACGTGGCGAGCCATTGTCTTTCCGTCTGGATGGCGTTATCCCAGGCTGGACTGAAGGCCTG 735 2 346 .......................................................................................... 257 0 445 .......................................................................................... 534 K Q I K K G G K I T L V I P P E L A Y G K A G V P G I P A N Query_1 736 AAGCAAATCAAAAAAGGTGGCAAAATCACCTTGGTTATCCCGCCAGAACTGGCTTACGGCAAAGCAGGCGTCCCTGGTATTCCTGCCAAC 825 2 256 .......................................................................................... 167 0 535 .......................................................................................... 624 S T L I F D V E L L D V K A A A K A D A Q E Q Q P A V D T K Query_1 826 TCTACTCTGATATTCGATGTAGAACTGCTGGATGTGAAAGCCGCAGCTAAAGCCGATGCTCAAGAGCAACAGCCTGCGGTTGATACAAAA 915 2 166 .......................................................................................... 77 0 625 .......................................................................................... 714 A K K * H W K W I T D P N S S S V D K L A A A L E H H H H H Query_1 916 GCTAAGAAGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccac 1005 2 76 ............................. 48 0 715 ......... 723 H * D P A A N K Query_1 1006 cacTGAGATCCGGCTGCTAACAAA 1029 2 46 ................. 30 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2445261 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 2,242 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP91528.102 (IDP91528.24-261.P(261)..pMCSG68)
ADTASASLSDAQKKEIEKVIHDYLINNPEVLLEASQALQQKQQQNMQQQAQAAIQENAEQVFQGKLTTVGNPKGNVTLVEFFDYQCIHCKKMASTIENLVKKDSGLRVIYKEFPIFGKTSDLASRVALAAGMQGKYQAMHNALITIDKRLDEKTVMDAAKSIGLDMQKLKKDMDSQEVTDILDANRQLAEKLHLMGTPAFIIGSTPDGQYKKGSEISFIPGATSEQSLRELIKKAAGN
GCTGATACGGCTAGCGCTTCTTTATCAGATGCTCAAAAAAAGGAAATTGAAAAAGTCATTCATGATTATCTGATTAATAACCCGGAAGTTTTATTGGAGGCTTCGCAAGCATTGCAACAAAAACAGCAACAAAATATGCAACAGCAAGCTCAAGCCGCAATTCAGGAAAATGCTGAACAAGTTTTTCAAGGCAAATTAACTACTGTTGGTAATCCTAAAGGGAATGTAACACTGGTTGAGTTTTTTGATTATCAATGCATTCATTGCAAAAAAATGGCATCTACCATTGAAAATCTGGTTAAAAAAGACAGCGGATTACGAGTTATATACAAAGAATTTCCAATCTTTGGCAAGACTTCTGATCTGGCTTCCAGAGTTGCTCTGGCGGCTGGCATGCAAGGCAAATATCAGGCTATGCATAATGCTTTGATTACGATAGACAAACGTTTGGATGAAAAAACAGTGATGGATGCCGCAAAATCCATAGGTTTGGATATGCAAAAGCTGAAAAAAGATATGGATAGTCAGGAAGTGACCGATATCCTGGATGCGAATCGCCAATTGGCTGAAAAATTACATTTGATGGGTACACCAGCCTTTATAATTGGCTCAACACCAGATGGTCAATATAAAAAGGGCAGTGAAATATCATTTATACCAGGCGCTACAAGCGAGCAATCACTACGTGAGTTAATTAAAAAGGCGGCTGGCAAT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 1,849 total letters Query= IDP91528.102 Length=1119 Score E Sequences producing significant alignments: (Bits) Value 1_01C_C1_IDP91528.102_T7sh.seq 1658 0.0 0_wt 1288 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 60 CCCCTC-TAGAAATAATTTTGTTT-AACTTT-AAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTG 146 1 19 ......C.................T......N.......................................................... 108 W S H P Q F E K G T E N L Y F Q S N A A D T A S A S L S D A Query_1 147 GTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGCTGATACGGCTAGCGCTTCTTTATCAGATGCTCa 236 1 109 .......................................................................................C.. 198 0 1 ................................... 35 Q K K E I E K V I H D Y L I N N P E V L L E A S Q A L Q Q K Query_1 237 aaaaaaGGAAATTGAAAAAGTCATTCATGATTATCTGATTAATAACCCGGAAGTTTTATTGGAGGCTTCGCAAGCATTGCAACAAAAACA 326 1 199 ..............................................................................A........... 288 0 36 .......................................................................................... 125 Q Q Q N M Q Q Q A Q A A I Q E N A E Q V F Q G K L T T V G N Query_1 327 GCAACAAAATATGCAACAGCAAGCTCAAGCCGCAATTCAGGAAAATGCTGAACAAGTTTTTCAAGGCAAATTAACTACTGTTGGTAATCC 416 1 289 ...G..........................T.......................G..........................A........ 378 0 126 .......................................................................................... 215 P K G N V T L V E F F D Y Q C I H C K K M A S T I E N L V K Query_1 417 TAAAGGGAATGTAACACTGGTTGAGTTTTTTGATTATCAATGCATTCATTGCaaaaaaaTGGCATCTACCATTGAAAATCTGGTTAAAAA 506 1 379 .......................................................................................... 468 0 216 .......................................................................................... 305 K D S G L R V I Y K E F P I F G K T S D L A S R V A L A A G Query_1 507 AGACAGCGGATTACGAGTTATATACAAAGAATTTCCAATCTTTGGCAAGACTTCTGATCTGGCTTCCAGAGTTGCTCTGGCGGCTGGCAT 596 1 469 ........................................................................................N. 558 1 867 ........... 877 0 306 .......................................................................................... 395 0 702 ........... 712 M Q G K Y Q A M H N A L I T I D K R L D E K T V M D A A K S Query_1 597 GCAAGGCAAATATCAGGCTATGCATAATGCTTTGATTACGATAGACAAACGTTTGGATGAAAAAACAGTGATGGATGCCGCAAAATCCAT 686 1 559 ......A........N.................................................................C........ 648 0 396 .......................................................................................... 485 I G L D M Q K L K K D M D S Q E V T D I L D A N R Q L A E K Query_1 687 AGGTTTGGATATGCAAAAGCTGAAAAAAGATATGGATAGTCAGGAAGTGACCGATATCCTGGATGCGAATCGCCAATTGGCTG-AAAAAT 775 1 649 ....C.............A.......................N.......................N.........C......A...... 738 0 486 ...................................................................................-...... 574 L H L M G T P A F I I G S T P D G Q Y K K G S E I S F I P Query_1 776 TACA-TTTGATGGGTACACCAGC-CTTTATAATTGGCTCAACACCAGATGGTCAATATAAAAAGGGCAGTGAAATATCATTTATACCAGG 863 1 739 ....T..................N................N..........G...................................... 828 0 575 ....-..................-.................................................................. 662 G A T S E Q S L R E L I K K A A G N * H W K W I T D P N S Query_1 864 CGCTACAAGCGAGCAATCACTACGTGAGTTAATTAAAAAGGCGGCTGGCAATT-AACATT-GGAAGTGGATAACGGATCCGAATT-CGAG 950 1 829 ......-..............................................A......G......C.................C.... 917 0 663 .................................................... 714 0 384 ........... 394 S S V D K L A A A L E H H H H H H * D P A A N K A R K E A E Query_1 951 CTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTG-CTAACAAAGCCCGAAA-GGAAGCTGAG 1038 1 918 ...............GC.............................T...............C................G.......... 1007 L A A A T A E Q * L A * P L G A S K R V L R G F L L Query_1 1039 TTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAA 1118 1 1008 ..-.N......NC.T....NA....-.N......-....TN...N........--C.................N...... 1082 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1991616 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 1,849 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP91528.103 (IDP91528.24-261.P(261)..pMCSG73)
ADTASASLSDAQKKEIEKVIHDYLINNPEVLLEASQALQQKQQQNMQQQAQAAIQENAEQVFQGKLTTVGNPKGNVTLVEFFDYQCIHCKKMASTIENLVKKDSGLRVIYKEFPIFGKTSDLASRVALAAGMQGKYQAMHNALITIDKRLDEKTVMDAAKSIGLDMQKLKKDMDSQEVTDILDANRQLAEKLHLMGTPAFIIGSTPDGQYKKGSEISFIPGATSEQSLRELIKKAAGN
GCTGATACGGCTAGCGCTTCTTTATCAGATGCTCAAAAAAAGGAAATTGAAAAAGTCATTCATGATTATCTGATTAATAACCCGGAAGTTTTATTGGAGGCTTCGCAAGCATTGCAACAAAAACAGCAACAAAATATGCAACAGCAAGCTCAAGCCGCAATTCAGGAAAATGCTGAACAAGTTTTTCAAGGCAAATTAACTACTGTTGGTAATCCTAAAGGGAATGTAACACTGGTTGAGTTTTTTGATTATCAATGCATTCATTGCAAAAAAATGGCATCTACCATTGAAAATCTGGTTAAAAAAGACAGCGGATTACGAGTTATATACAAAGAATTTCCAATCTTTGGCAAGACTTCTGATCTGGCTTCCAGAGTTGCTCTGGCGGCTGGCATGCAAGGCAAATATCAGGCTATGCATAATGCTTTGATTACGATAGACAAACGTTTGGATGAAAAAACAGTGATGGATGCCGCAAAATCCATAGGTTTGGATATGCAAAAGCTGAAAAAAGATATGGATAGTCAGGAAGTGACCGATATCCTGGATGCGAATCGCCAATTGGCTGAAAAATTACATTTGATGGGTACACCAGCCTTTATAATTGGCTCAACACCAGATGGTCAATATAAAAAGGGCAGTGAAATATCATTTATACCAGGCGCTACAAGCGAGCAATCACTACGTGAGTTAATTAAAAAGGCGGCTGGCAAT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 1,321 total letters Query= IDP91528.103 Length=1119 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1288 0.0 2_12C_C6_IDP91528.103_T7term.seq 942 0.0 A D T A S A S L S D A Q K K E I E K V I H D Y L I N N P E V Query_1 202 GCTGATACGGCTAGCGCTTCTTTATCAGATGCTCaaaaaaaGGAAATTGAAAAAGTCATTCATGATTATCTGATTAATAACCCGGAAGTT 291 0 1 .......................................................................................... 90 L L E A S Q A L Q Q K Q Q Q N M Q Q Q A Q A A I Q E N A E Q Query_1 292 TTATTGGAGGCTTCGCAAGCATTGCAACAAAAACAGCAACAAAATATGCAACAGCAAGCTCAAGCCGCAATTCAGGAAAATGCTGAACAA 381 0 91 .......................................................................................... 180 V F Q G K L T T V G N P K G N V T L V E F F D Y Q C I H C K Query_1 382 GTTTTTCAAGGCAAATTAACTACTGTTGGTAATCCTAAAGGGAATGTAACACTGGTTGAGTTTTTTGATTATCAATGCATTCATTGCaaa 471 0 181 .......................................................................................... 270 2 595 ..........................A........................N...................................... 506 K M A S T I E N L V K K D S G L R V I Y K E F P I F G K T S Query_1 472 aaaaTGGCATCTACCATTGAAAATCTGGTTAAAAAAGACAGCGGATTACGAGTTATATACAAAGAATTTCCAATCTTTGGCAAGACTTCT 561 0 271 .......................................................................................... 360 2 505 .....................................G.................................................... 416 D L A S R V A L A A G M Q G K Y Q A M H N A L I T I D K R L Query_1 562 GATCTGGCTTCCAGAGTTGCTCTGGCGGCTGGCATGCAAGGCAAATATCAGGCTATGCATAATGCTTTGATTACGATAGACAAACGTTTG 651 0 361 .......................................................................................... 450 0 702 ........... 712 2 415 .........................N.............N.A................................................ 326 2 74 ........... 64 D E K T V M D A A K S I G L D M Q K L K K D M D S Q E V T D Query_1 652 GATGAAAAAACAGTGATGGATGCCGCAAAATCCATAGGTTTGGATATGCAAAAGCTGAAAAAAGATATGGATAGTCAGGAAGTGACCGAT 741 0 451 .......................................................................................... 540 2 325 ..........................C............C.............A.................................... 236 I L D A N R Q L A E K L H L M G T P A F I I G S T P D G Q Y Query_1 742 ATCCTGGATGCGAATCGCCAATTGGCTGAAAAATTACATTTGATGGGTACACCAGCCTTTATAATTGGCTCAACACCAGATGGTCAATAT 831 0 541 .......................................................................................... 630 2 235 .....................C.............................................................G...... 146 K K G S E I S F I P G A T S E Q S L R E L I K K A A G N * H Query_1 832 AAAAAGGGCAGTGAAATATCATTTATACCAGGCGCTACAAGCGAGCAATCACTACGTGAGTTAATTAAAAAGGCGGCTGGCAATTAACAT 921 0 631 .................................................................................... 714 0 384 ........... 394 2 145 ......................................................................................N..- 57 W K W I T D P N S S S V D K L A A A L E H H H H H H * D P A Query_1 922 TGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCT 1011 2 56 ..............--..... 38 2 42 ........ 35 A N K A R Query_1 1012 GCTAACAAAGCCCGAAA 1028 2 34 .........N....... 18 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1413295 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 1,321 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95771.103 (IDP95771.19-628.P(628)..pMCSG68)
AKKLAKKYKKDKDVNITLIDRHSYHTMMTELHEVAGGRVEPTAIQYDLRRLFNRTKVNLVTDNVTHVDHDKKVVTTEHGSYPFDYLVLGMGGEPNDFGTPGVGENGFTLWSWEDSVKLRNHIEETVTKASREQDVEKRKAMLTFVVCGSGFTGIEMVGELLEWKDRLAKDNKIDASEIKLVVVEAAPTILNMLERRDADKAERYMVKKGIEIMKNAAIVEVKPESIVLKSGEELPTSTLIWTAGVRANSDTKDYGMESARAGRLKVNQYMEAEGLKDVYVVGDLAYFEDEEGKPTPQIVEGAEQTALTAAKSIIVEMSGTGEKEPFQGKYHGVMVSIGAKYGVAHLGGMHLSGWFAILMKHMVNLYYFFGIRSGYYMWQYIMHEFFHIKDHRNIFRGWTSRYGNVLWVLPLRVYLGWFWIDEALSKIYGETTWDKVSITNLKPLFNGIGSDSWLTATTSKMPFEWLQTAATSGASQAAGDAAGAAATNVTTPILSHMPGWFQWIMELLMPNLDVALVMQKVVPFVELAIGLAMVVGLFTWLVSIGSAGFLVMFTLSAMLGWDKFWALPASIALLNGAGRTFGLDYWAVPWFQKHLGHWWYGKPRSVYRDK
GCTAAGAAATTAGCTAAGAAATACAAGAAAGACAAAGACGTTAATATTACATTAATCGATCGTCATTCGTACCATACAATGATGACTGAACTACATGAGGTTGCTGGTGGTCGTGTTGAACCAACTGCAATTCAATATGATTTACGTCGTTTGTTTAATAGAACAAAAGTTAATCTTGTAACTGATAACGTGACACATGTAGATCATGATAAAAAAGTTGTAACAACAGAGCACGGTAGTTATCCGTTTGATTACCTAGTACTTGGTATGGGCGGAGAACCTAATGATTTCGGAACTCCTGGTGTTGGCGAAAATGGCTTTACACTTTGGTCTTGGGAAGATTCTGTTAAGTTACGCAATCATATTGAAGAAACTGTAACGAAAGCATCTCGCGAACAAGACGTTGAAAAACGTAAAGCGATGTTAACATTCGTTGTTTGTGGATCTGGATTTACTGGTATCGAAATGGTTGGGGAACTTTTAGAATGGAAAGATCGTCTTGCTAAAGATAACAAAATTGACGCATCTGAAATTAAACTAGTTGTAGTAGAAGCTGCTCCAACAATCCTAAACATGCTTGAAAGAAGAGACGCTGACAAAGCAGAACGTTACATGGTTAAAAAAGGTATTGAAATCATGAAAAATGCTGCTATCGTTGAAGTTAAACCTGAAAGCATCGTACTTAAATCTGGCGAAGAACTTCCAACAAGCACATTAATTTGGACTGCTGGTGTTCGCGCTAACTCCGATACAAAAGATTACGGTATGGAATCTGCTCGTGCAGGCCGTTTGAAAGTAAACCAATATATGGAAGCAGAAGGTCTTAAAGACGTTTATGTTGTTGGTGACCTTGCTTACTTTGAAGATGAAGAAGGCAAACCAACTCCGCAAATCGTTGAAGGTGCTGAACAAACTGCACTAACTGCAGCGAAAAGTATCATCGTTGAAATGAGCGGTACTGGCGAGAAAGAACCATTCCAAGGTAAATACCATGGTGTAATGGTTTCTATTGGAGCTAAATATGGTGTTGCTCATCTTGGTGGCATGCATCTTTCTGGTTGGTTCGCTATTTTAATGAAACATATGGTTAACTTGTACTACTTCTTCGGTATCCGTAGTGGTTACTACATGTGGCAATATATTATGCATGAATTCTTCCACATTAAAGATCACCGTAATATCTTCCGTGGTTGGACTTCTCGTTACGGTAACGTACTTTGGGTTCTTCCTTTACGTGTATATCTAGGTTGGTTCTGGATTGACGAAGCTCTTTCTAAAATCTACGGTGAAACTACATGGGATAAAGTAAGTATCACAAATTTAAAACCTTTATTTAACGGTATCGGTTCTGACTCTTGGTTAACTGCAACTACCTCTAAAATGCCATTCGAATGGTTACAAACTGCTGCTACATCTGGTGCTAGTCAAGCTGCCGGTGATGCTGCTGGGGCTGCTGCAACAAACGTAACTACTCCAATTCTTAGTCATATGCCTGGTTGGTTCCAGTGGATTATGGAACTTCTAATGCCAAATCTTGATGTTGCTCTAGTAATGCAAAAAGTTGTACCTTTTGTTGAACTTGCAATCGGTCTTGCTATGGTAGTAGGTCTATTCACTTGGCTTGTAAGTATCGGAAGTGCTGGATTCCTAGTAATGTTTACACTAAGCGCTATGCTTGGTTGGGACAAATTCTGGGCGTTACCAGCTTCTATCGCACTTCTAAATGGCGCTGGGCGTACATTTGGTCTTGATTATTGGGCTGTTCCTTGGTTCCAAAAACATCTTGGTCATTGGTGGTACGGTAAGCCGAGATCCGTTTACAGAGACAAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 1,840 total letters Query= IDP95771.103 Length=2235 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3301 0.0 A K K L A K K Y K K D K D V N I T L I D R H S Y H T M M T E Query_1 202 GCTAAGAAATTAGCTAAGAAATACAAGAAAGACAAAGACGTTAATATTACATTAATCGATCGTCATTCGTACCATACAATGATGACTGAA 291 0 1 .......................................................................................... 90 L H E V A G G R V E P T A I Q Y D L R R L F N R T K V N L V Query_1 292 CTACATGAGGTTGCTGGTGGTCGTGTTGAACCAACTGCAATTCAATATGATTTACGTCGTTTGTTTAATAGAACAAAAGTTAATCTTGTA 381 0 91 .......................................................................................... 180 0 369 ............ 358 T D N V T H V D H D K K V V T T E H G S Y P F D Y L V L G M Query_1 382 ACTGATAACGTGACACATGTAGATCATGATAAAAAAGTTGTAACAACAGAGCACGGTAGTTATCCGTTTGATTACCTAGTACTTGGTATG 471 0 181 .......................................................................................... 270 0 1556 ........... 1566 G G E P N D F G T P G V G E N G F T L W S W E D S V K L R N Query_1 472 GGCGGAGAACCTAATGATTTCGGAACTCCTGGTGTTGGCGAAAATGGCTTTACACTTTGGTCTTGGGAAGATTCTGTTAAGTTACGCAAT 561 0 271 .......................................................................................... 360 0 142 ... 140 H I E E T V T K A S R E Q D V E K R K A M L T F V V C G S G Query_1 562 CATATTGAAGAAACTGTAACGAAAGCATCTCGCGAACAAGACGTTGAAAAACGTAAAGCGATGTTAACATTCGTTGTTTGTGGATCTGGA 651 0 361 .......................................................................................... 450 0 139 ......... 131 F T G I E M V G E L L E W K D R L A K D N K I D A S E I K L Query_1 652 TTTACTGGTATCGAAATGGTTGGGGAACTTTTAGAATGGAAAGATCGTCTTGCTAAAGATAACAAAATTGACGCATCTGAAATTAAACTA 741 0 451 .......................................................................................... 540 V V V E A A P T I L N M L E R R D A D K A E R Y M V K K G I Query_1 742 GTTGTAGTAGAAGCTGCTCCAACAATCCTAAACATGCTTGAAAGAAGAGACGCTGACAAAGCAGAACGTTACATGGTTAAAAAAGGTATT 831 0 541 .......................................................................................... 630 E I M K N A A I V E V K P E S I V L K S G E E L P T S T L I Query_1 832 GAAATCATGAAAAATGCTGCTATCGTTGAAGTTAAACCTGAAAGCATCGTACTTAAATCTGGCGAAGAACTTCCAACAAGCACATTAATT 921 0 631 .......................................................................................... 720 W T A G V R A N S D T K D Y G M E S A R A G R L K V N Q Y M Query_1 922 TGGACTGCTGGTGTTCGCGCTAACTCCGATACAAAAGATTACGGTATGGAATCTGCTCGTGCAGGCCGTTTGAAAGTAAACCAATATATG 1011 0 721 .......................................................................................... 810 E A E G L K D V Y V V G D L A Y F E D E E G K P T P Q I V E Query_1 1012 GAAGCAGAAGGTCTTAAAGACGTTTATGTTGTTGGTGACCTTGCTTACTTTGAAGATGAAGAAGGCAAACCAACTCCGCAAATCGTTGAA 1101 0 811 .......................................................................................... 900 G A E Q T A L T A A K S I I V E M S G T G E K E P F Q G K Y Query_1 1102 GGTGCTGAACAAACTGCACTAACTGCAGCGAAAAGTATCATCGTTGAAATGAGCGGTACTGGCGAGAAAGAACCATTCCAAGGTAAATAC 1191 0 901 .......................................................................................... 990 H G V M V S I G A K Y G V A H L G G M H L S G W F A I L M K Query_1 1192 CATGGTGTAATGGTTTCTATTGGAGCTAAATATGGTGTTGCTCATCTTGGTGGCATGCATCTTTCTGGTTGGTTCGCTATTTTAATGAAA 1281 0 991 .......................................................................................... 1080 0 1493 ........... 1503 H M V N L Y Y F F G I R S G Y Y M W Q Y I M H E F F H I K D Query_1 1282 CATATGGTTAACTTGTACTACTTCTTCGGTATCCGTAGTGGTTACTACATGTGGCAATATATTATGCATGAATTCTTCCACATTAAAGAT 1371 0 1081 .......................................................................................... 1170 H R N I F R G W T S R Y G N V L W V L P L R V Y L G W F W I Query_1 1372 CACCGTAATATCTTCCGTGGTTGGACTTCTCGTTACGGTAACGTACTTTGGGTTCTTCCTTTACGTGTATATCTAGGTTGGTTCTGGATT 1461 0 1171 .......................................................................................... 1260 D E A L S K I Y G E T T W D K V S I T N L K P L F N G I G S Query_1 1462 GACGAAGCTCTTTCTAAAATCTACGGTGAAACTACATGGGATAAAGTAAGTATCACAAATTTAAAACCTTTATTTAACGGTATCGGTTCT 1551 0 1261 .......................................................................................... 1350 D S W L T A T T S K M P F E W L Q T A A T S G A S Q A A G D Query_1 1552 GACTCTTGGTTAACTGCAACTACCTCTAAAATGCCATTCGAATGGTTACAAACTGCTGCTACATCTGGTGCTAGTCAAGCTGCCGGTGAT 1641 0 1351 .......................................................................................... 1440 0 1395 ............ 1384 A A G A A A T N V T T P I L S H M P G W F Q W I M E L L M P Query_1 1642 GCTGCTGGGGCTGCTGCAACAAACGTAACTACTCCAATTCTTAGTCATATGCCTGGTTGGTTCCAGTGGATTATGGAACTTCTAATGCCA 1731 0 1441 .......................................................................................... 1530 0 1055 ........... 1065 N L D V A L V M Q K V V P F V E L A I G L A M V V G L F T W Query_1 1732 AATCTTGATGTTGCTCTAGTAATGCAAAAAGTTGTACCTTTTGTTGAACTTGCAATCGGTCTTGCTATGGTAGTAGGTCTATTCACTTGG 1821 0 1531 .......................................................................................... 1620 0 212 ........... 222 L V S I G S A G F L V M F T L S A M L G W D K F W A L P A S Query_1 1822 CTTGTAAGTATCGGAAGTGCTGGATTCCTAGTAATGTTTACACTAAGCGCTATGCTTGGTTGGGACAAATTCTGGGCGTTACCAGCTTCT 1911 0 1621 .......................................................................................... 1710 I A L L N G A G R T F G L D Y W A V P W F Q K H L G H W W Y Query_1 1912 ATCGCACTTCTAAATGGCGCTGGGCGTACATTTGGTCTTGATTATTGGGCTGTTCCTTGGTTCCAAAAACATCTTGGTCATTGGTGGTAC 2001 0 1711 .......................................................................................... 1800 G K P R S V Y R D K Query_1 2002 GGTAAGCCGAGATCCGTTTACAGAGACAAG 2031 0 1801 .............................. 1830 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3976448 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 1,840 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95810.102 (IDP95810.1-377.F..pMCSG68)
MMEFTIKRDYFITQLNDTLKAISPRTTLPILTGIKIDAKEHEVILTGSDSEISIEITIPKTVDGEDIVNISETGSVVLPGRFFVDIIKKLPGKDVKLSTNEQFQTLITSGHSEFNLSGLDPDQYPLLPQVSRDDAIQLSVKVLKNVIAQTNFAVSTSETRPVLTGVNWLIQENELICTATDSHRLAVRKLQLEDVSENKNVIIPGKALAELNKIMSDNEEDIDIFFASNQVLFKVGNVNFISRLLEGHYPDTTRLFPENYEIKLSIDNGEFYHAIDRASLLAREGGNNVIKLSTGDDVVELSSTSPEIGTVKEEVDANDVEGGSLKISFNSKYMMDALKAIDNDEVEVEFFGTMKPFILKPKGDDSVTQLILPIRTY
ATGATGGAATTCACTATTAAAAGAGATTATTTTATTACACAATTAAATGACACATTAAAAGCTATTTCACCAAGAACAACATTACCTATATTAACTGGTATCAAAATCGATGCGAAAGAACATGAAGTTATATTAACTGGTTCAGACTCTGAAATTTCAATAGAAATCACTATTCCTAAAACTGTAGATGGCGAAGATATTGTCAATATTTCAGAAACAGGCTCAGTAGTACTTCCTGGACGATTCTTTGTTGATATTATAAAAAAATTACCTGGTAAAGATGTTAAATTATCTACAAATGAACAATTCCAGACATTAATTACATCAGGTCATTCTGAATTTAATTTAAGTGGCTTAGATCCAGATCAATATCCTTTATTACCTCAAGTTTCTAGAGATGACGCAATTCAATTGTCGGTAAAAGTGCTTAAAAACGTGATTGCACAAACAAATTTTGCAGTGTCCACCTCAGAAACACGCCCAGTACTAACTGGTGTGAACTGGCTTATACAAGAAAATGAATTAATATGCACAGCGACTGACTCACACCGCTTGGCTGTAAGAAAGTTGCAGTTAGAAGATGTTTCTGAAAACAAAAATGTCATCATTCCAGGTAAGGCTTTAGCTGAATTAAATAAAATTATGTCTGACAATGAAGAAGACATTGATATCTTCTTTGCTTCAAACCAAGTTTTATTTAAAGTTGGAAATGTGAACTTTATTTCTCGATTATTAGAAGGACATTATCCTGATACAACACGTTTATTCCCTGAAAACTATGAAATTAAATTAAGTATAGACAATGGGGAGTTTTATCATGCGATTGATCGTGCCTCTTTATTAGCGCGTGAAGGTGGTAATAACGTTATTAAATTAAGTACAGGTGATGACGTTGTTGAATTGTCTTCTACATCACCAGAAATTGGTACTGTAAAAGAAGAAGTTGATGCAAACGATGTTGAAGGTGGTAGCCTGAAAATTTCATTCAACTCTAAATATATGATGGATGCTTTAAAAGCAATCGATAATGATGAGGTTGAAGTTGAATTCTTCGGTACAATGAAACCATTTATTCTAAAACCAAAAGGTGACGACTCGGTAACGCAATTAATTTTACCAATCAGAACTTAC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 1,141 total letters Query= IDP95810.102 Length=1536 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2040 0.0 M M E F T I K R D Y F I T Q L N D T L K A I S P R T T L P I Query_1 202 ATGATGGAATTCACTATTAAAAGAGATTATTTTATTACACAATTAAATGACACATTAAAAGCTATTTCACCAAGAACAACATTACCTATA 291 0 1 .......................................................................................... 90 L T G I K I D A K E H E V I L T G S D S E I S I E I T I P K Query_1 292 TTAACTGGTATCAAAATCGATGCGAAAGAACATGAAGTTATATTAACTGGTTCAGACTCTGAAATTTCAATAGAAATCACTATTCCTAAA 381 0 91 .......................................................................................... 180 T V D G E D I V N I S E T G S V V L P G R F F V D I I K K L Query_1 382 ACTGTAGATGGCGAAGATATTGTCAATATTTCAGAAACAGGCTCAGTAGTACTTCCTGGACGATTCTTTGTTGATATTATaaaaaaaTTA 471 0 181 .......................................................................................... 270 0 592 ........... 582 P G K D V K L S T N E Q F Q T L I T S G H S E F N L S G L D Query_1 472 CCTGGTAAAGATGTTAAATTATCTACAAATGAACAATTCCAGACATTAATTACATCAGGTCATTCTGAATTTAATTTAAGTGGCTTAGAT 561 0 271 .......................................................................................... 360 P D Q Y P L L P Q V S R D D A I Q L S V K V L K N V I A Q T Query_1 562 CCAGATCAATATCCTTTATTACCTCAAGTTTCTAGAGATGACGCAATTCAATTGTCGGTAAAAGTGCTTAAAAACGTGATTGCACAAACA 651 0 361 .......................................................................................... 450 N F A V S T S E T R P V L T G V N W L I Q E N E L I C T A T Query_1 652 AATTTTGCAGTGTCCACCTCAGAAACACGCCCAGTACTAACTGGTGTGAACTGGCTTATACAAGAAAATGAATTAATATGCACAGCGACT 741 0 451 .......................................................................................... 540 D S H R L A V R K L Q L E D V S E N K N V I I P G K A L A E Query_1 742 GACTCACACCGCTTGGCTGTAAGAAAGTTGCAGTTAGAAGATGTTTCTGAAAACAAAAATGTCATCATTCCAGGTAAGGCTTTAGCTGAA 831 0 541 .......................................................................................... 630 0 219 ........... 209 L N K I M S D N E E D I D I F F A S N Q V L F K V G N V N F Query_1 832 TTAAATAAAATTATGTCTGACAATGAAGAAGACATTGATATCTTCTTTGCTTCAAACCAAGTTTTATTTAAAGTTGGAAATGTGAACTTT 921 0 631 .......................................................................................... 720 I S R L L E G H Y P D T T R L F P E N Y E I K L S I D N G E Query_1 922 ATTTCTCGATTATTAGAAGGACATTATCCTGATACAACACGTTTATTCCCTGAAAACTATGAAATTAAATTAAGTATAGACAATGGGGAG 1011 0 721 .......................................................................................... 810 0 868 .............C.. 883 F Y H A I D R A S L L A R E G G N N V I K L S T G D D V V E Query_1 1012 TTTTATCATGCGATTGATCGTGCCTCTTTATTAGCGCGTGAAGGTGGTAATAACGTTATTAAATTAAGTACAGGTGATGACGTTGTTGAA 1101 0 811 .......................................................................................... 900 0 784 .............T.. 799 0 871 .............. 858 0 960 ........... 970 L S S T S P E I G T V K E E V D A N D V E G G S L K I S F N Query_1 1102 TTGTCTTCTACATCACCAGAAATTGGTACTGTAAAAGAAGAAGTTGATGCAAACGATGTTGAAGGTGGTAGCCTGAAAATTTCATTCAAC 1191 0 901 .......................................................................................... 990 0 849 ........... 859 S K Y M M D A L K A I D N D E V E V E F F G T M K P F I L K Query_1 1192 TCTAAATATATGATGGATGCTTTAAAAGCAATCGATAATGATGAGGTTGAAGTTGAATTCTTCGGTACAATGAAACCATTTATTCTAAAA 1281 0 991 .......................................................................................... 1080 P K G D D S V T Q L I L P I R T Y Query_1 1282 CCAAAAGGTGACGACTCGGTAACGCAATTAATTTTACCAATCAGAACTTAC 1332 0 1081 ................................................... 1131 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1667016 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 1,141 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95810.103 (IDP95810.1-377.F..pMCSG73)
MMEFTIKRDYFITQLNDTLKAISPRTTLPILTGIKIDAKEHEVILTGSDSEISIEITIPKTVDGEDIVNISETGSVVLPGRFFVDIIKKLPGKDVKLSTNEQFQTLITSGHSEFNLSGLDPDQYPLLPQVSRDDAIQLSVKVLKNVIAQTNFAVSTSETRPVLTGVNWLIQENELICTATDSHRLAVRKLQLEDVSENKNVIIPGKALAELNKIMSDNEEDIDIFFASNQVLFKVGNVNFISRLLEGHYPDTTRLFPENYEIKLSIDNGEFYHAIDRASLLAREGGNNVIKLSTGDDVVELSSTSPEIGTVKEEVDANDVEGGSLKISFNSKYMMDALKAIDNDEVEVEFFGTMKPFILKPKGDDSVTQLILPIRTY
ATGATGGAATTCACTATTAAAAGAGATTATTTTATTACACAATTAAATGACACATTAAAAGCTATTTCACCAAGAACAACATTACCTATATTAACTGGTATCAAAATCGATGCGAAAGAACATGAAGTTATATTAACTGGTTCAGACTCTGAAATTTCAATAGAAATCACTATTCCTAAAACTGTAGATGGCGAAGATATTGTCAATATTTCAGAAACAGGCTCAGTAGTACTTCCTGGACGATTCTTTGTTGATATTATAAAAAAATTACCTGGTAAAGATGTTAAATTATCTACAAATGAACAATTCCAGACATTAATTACATCAGGTCATTCTGAATTTAATTTAAGTGGCTTAGATCCAGATCAATATCCTTTATTACCTCAAGTTTCTAGAGATGACGCAATTCAATTGTCGGTAAAAGTGCTTAAAAACGTGATTGCACAAACAAATTTTGCAGTGTCCACCTCAGAAACACGCCCAGTACTAACTGGTGTGAACTGGCTTATACAAGAAAATGAATTAATATGCACAGCGACTGACTCACACCGCTTGGCTGTAAGAAAGTTGCAGTTAGAAGATGTTTCTGAAAACAAAAATGTCATCATTCCAGGTAAGGCTTTAGCTGAATTAAATAAAATTATGTCTGACAATGAAGAAGACATTGATATCTTCTTTGCTTCAAACCAAGTTTTATTTAAAGTTGGAAATGTGAACTTTATTTCTCGATTATTAGAAGGACATTATCCTGATACAACACGTTTATTCCCTGAAAACTATGAAATTAAATTAAGTATAGACAATGGGGAGTTTTATCATGCGATTGATCGTGCCTCTTTATTAGCGCGTGAAGGTGGTAATAACGTTATTAAATTAAGTACAGGTGATGACGTTGTTGAATTGTCTTCTACATCACCAGAAATTGGTACTGTAAAAGAAGAAGTTGATGCAAACGATGTTGAAGGTGGTAGCCTGAAAATTTCATTCAACTCTAAATATATGATGGATGCTTTAAAAGCAATCGATAATGATGAGGTTGAAGTTGAATTCTTCGGTACAATGAAACCATTTATTCTAAAACCAAAAGGTGACGACTCGGTAACGCAATTAATTTTACCAATCAGAACTTAC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,995 total letters Query= IDP95810.103 Length=1536 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2040 0.0 2_07A_A8_IDP95810.103_T7term.seq 1772 0.0 1_05A_A8_IDP95810.103_v73F.seq 967 0.0 W S H P Q F E K G T E N L Y F Q S N A M M E F T I K R D Y Query_1 145 TGGTCTCATCCGCAGTT-CGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGATGGAATTCACT--ATTAAAAGAGATTAT 231 0 1 ...............--............... 30 2 1219 ............GTC.NN.....NNN.GT. 1190 2 1048 ..A...N.....--... 1034 1 44 ...........A.....T...G....------.........................................--............... 125 F I T Q L N D T L K A I S P R T T L P I L T G I K I D Query_1 232 TTTA-TTACACAATTAAATGACACATTAAAAGCTATTTCACCAAGAA-CAA-CATTA-CCTATATT-AACTGGTA-TCAAAATC-GATGC 314 0 31 ....-..........................................-...-.....-........-........-........-..... 113 2 1189 ....N....C..T..N.T..G.N.C.....G.N....CA...C....G..NN.NN..N........T.N......N.....T.NN.T..N 1100 1 126 ....-..........................................-...-.....-........-........-........-..... 208 A K E H E V I L T G S D S E I S I E I T I P K T V D G E Query_1 315 GAAAGA-AC-ATGAAGTTATAT--TAACTGGTTCA-GACTCTGAAATTTCAATAGAAATCACTATTCCT-AAAACTGTAGATGGCGAAGA 398 0 114 ......-..-............--...........-.................................-.................... 197 2 1099 .....NN..N........T...ACNNNN.......A.....................N...........T...................N 1010 1 209 ......-..-...........C--...........-.................................-.................... 292 D I V N I S E T G S V V L P G R F F V D I I K K L P G K D V Query_1 399 -TATTGTCAATATTT-CAGAAACAGGCTCAGTAGTACTTCCTGGACGATTCTTTGTTGATATTATaaaaaaaTTACCTGGTAAAGATGTT 486 0 198 -..............-.......................................................................... 285 0 592 ...-........ 582 2 1009 N..............T.....-...............................N.................................... 921 2 609 ...-........ 619 1 293 -..............-.......................................................................... 380 K L S T N E Q F Q T L I T S G H S E F N L S G L D P D Q Y Query_1 487 AAATTATCTAC-AAATGAACAATTCC-AGACATTAATTACATCAGG-TCATTCTGAATTTAATTTAAGTGGCTTAGATCCAGATCAATAT 573 0 286 ...........-..............-...................-........................................... 372 2 920 ...........N..............C...................G........................................... 831 1 381 ...........-..............-...................-........................................... 467 P L L P Q V S R D D A I Q L S V K V L K N V I A Q T N F A Query_1 574 CCTTTATTACCTCAAGTTTCTAGAGATGACGCAATTCAATTGTCGGTAAAAGTGCTTAAAAACGTGATTGCACAAA-CAAATTTTGCAGT 662 0 373 ............................................................................-............. 461 2 830 ............................................................................A.G........... 741 1 468 ....................................N.......................................-.G........... 556 V S T S E T R P V L T G V N W L I Q E N E L I C T A T D S H Query_1 663 GTCCACCTCAGAAA-CACGCCCAGTACTAACTGGTGTGAACTGGCTTATACAAGAAAATGAATTAATATGCACAGCGACTGACTCACACC 751 0 462 ..............-........................................................................... 550 2 740 ..............A........................................................................... 651 1 557 ..............-.................................. 604 1 226 ........... 236 R L A V R K L Q L E D V S E N K N V I I P G K A L A E L N K Query_1 752 GCTTGGCTGTAAGAAAGTTGCAGTTAGAAGATGTTTCTGAAAACAAAAATGTCATCATTCCAGGTAAGGCTTTAGCTGAATTAAATAAAA 841 0 551 .......................................................................................... 640 0 219 ........... 209 2 650 .......................................................................................... 561 1 314 ........... 304 I M S D N E E D I D I F F A S N Q V L F K V G N V N F I S R Query_1 842 TTATGTCTGACAATGAAGAAGACATTGATATCTTCTTTGCTTCAAACCAAGTTTTATTTAAAGTTGGAAATGTGAACTTTATTTCTCGAT 931 0 641 .......................................................................................... 730 2 560 .......................................................................................... 471 L L E G H Y P D T T R L F P E N Y E I K L S I D N G E F Y H Query_1 932 TATTAGAAGGACATTATCCTGATACAACACGTTTATTCCCTGAAAACTATGAAATTAAATTAAGTATAGACAATGGGGAGTTTTATCATG 1021 0 731 .......................................................................................... 820 0 868 .............C.. 883 2 470 .......................................................................................... 381 2 333 .............C.. 318 A I D R A S L L A R E G G N N V I K L S T G D D V V E L S S Query_1 1022 CGATTGATCGTGCCTCTTTATTAGCGCGTGAAGGTGGTAATAACGTTATTAAATTAAGTACAGGTGATGACGTTGTTGAATTGTCTTCTA 1111 0 821 .......................................................................................... 910 0 784 .............T.. 799 0 871 .............. 858 0 960 ........... 970 2 380 .........................A........................................................A....... 291 2 417 .............T.. 402 2 330 .............. 343 2 241 ........... 231 T S P E I G T V K E E V D A N D V E G G S L K I S F N S K Y Query_1 1112 CATCACCAGAAATTGGTACTGTAAAAGAAGAAGTTGATGCAAACGATGTTGAAGGTGGTAGCCTGAAAATTTCATTCAACTCTAAATATA 1201 0 911 .......................................................................................... 1000 0 849 ........... 859 2 290 .......................................................................................... 201 2 352 ........... 342 M M D A L K A I D N D E V E V E F F G T M K P F I L K P K G Query_1 1202 TGATGGATGCTTTAAAAGCAATCGATAATGATGAGGTTGAAGTTGAATTCTTCGGTACAATGAAACCATTTATTCTAAAACCAAAAGGTG 1291 0 1001 .......................................................................................... 1090 2 200 .......................................................................................... 111 D D S V T Q L I L P I R T Y * H W K W I T D P N S S S V D K Query_1 1292 ACGACTCGGTAACGCAATTAATTTTACCAATCAGAACTTACTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGC 1381 0 1091 ......................................... 1131 2 110 ............................................................ 51 L A A A L E H H H H H H * D P A A N K A R Query_1 1382 TTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAG-CCCGAAA 1445 2 48 ..................C....... 23 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4479440 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 2,995 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95831.103 (IDP95831.1-428.F..pMCSG73)
MEKDPIPQATSPLATWLSYLEHLHSKTIDLGLARVSEVAGQMDIVKPAPFVFTVAGTNGKGTTCRTLETILMAAGYKVGVYSSPHLVRYTERVRIQGAELPEAAHTASFAEIEAARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVDADVAVVTSIALDHTDWLGPDRESIGREKAGIFRAGKPAVVGEPDMPLTIAEVASEKGALLQRRGVDWRYEVEGETWSFRDTAGALSHLPLPQVPLPNAATAVAALRASGLAVDDAILRAGIRDAMLPGRFQIISDAPRVILDVAHNPHAAAYLAGRLKTLAKTGRVLAVIGMLHDKDIAGTLANLAPEVDAWYCAPLEGPRGATAEQLVEHLRCGTVYSSVAQAWRAAMADAKVEDTVLVCGSFHTVAQVMEEIDAGXNRWRVSFRTV
ATGGAAAAAGATCCTATTCCTCAGGCCACGTCGCCCCTGGCTACGTGGCTTTCTTATCTGGAACATCTGCACAGTAAAACCATCGACCTTGGGCTGGCGCGCGTCAGCGAGGTCGCCGGGCAGATGGATATCGTTAAACCGGCGCCGTTCGTTTTTACCGTTGCGGGCACCAATGGCAAAGGCACCACCTGCCGCACGCTGGAAACCATCCTGATGGCGGCCGGTTATAAAGTGGGGGTCTACAGCTCTCCGCATCTGGTGCGCTACACCGAACGCGTGCGCATTCAGGGGGCTGAACTGCCGGAGGCGGCGCATACAGCTTCGTTTGCTGAAATCGAAGCGGCGCGGGGTGACATCTCGCTGACCTATTTTGAATACGGCACGCTCTCGGCGCTGTGGCTGTTTAAGCAGGCGCAGCTGGATGTGGTGATCCTTGAGGTCGGGCTCGGCGGGCGTCTCGACGCCACCAACATCGTGGATGCCGACGTCGCGGTGGTCACCAGCATCGCCCTCGATCATACCGACTGGCTGGGCCCGGATCGCGAAAGTATCGGCCGGGAAAAAGCGGGCATCTTTCGCGCCGGTAAACCGGCGGTGGTGGGGGAGCCGGATATGCCGCTCACCATCGCGGAAGTGGCTAGCGAGAAGGGCGCGCTGCTCCAGCGCCGCGGCGTCGACTGGCGCTATGAGGTGGAGGGCGAAACGTGGTCCTTCCGCGATACGGCAGGGGCGTTAAGCCACCTGCCTCTGCCTCAGGTGCCGTTGCCGAATGCGGCGACGGCCGTGGCGGCGCTGCGCGCCAGCGGGCTTGCCGTGGATGACGCTATCCTGCGCGCAGGGATCCGCGATGCGATGCTCCCGGGACGCTTTCAGATAATCAGCGACGCCCCGCGAGTGATCCTCGATGTGGCGCACAACCCGCACGCGGCGGCCTATCTCGCCGGGCGTCTCAAAACGTTAGCGAAAACGGGACGCGTGCTGGCGGTTATTGGTATGCTGCATGATAAGGATATCGCCGGCACGTTGGCCAATCTGGCCCCGGAAGTCGATGCCTGGTACTGCGCGCCGCTGGAAGGCCCGCGCGGCGCGACGGCAGAGCAGCTGGTGGAACATTTGCGCTGCGGTACAGTCTATAGCAGCGTGGCGCAGGCGTGGCGTGCGGCCATGGCTGACGCTAAAGTGGAAGATACCGTGCTGGTGTGTGGTTCGTTTCACACCGTAGCGCAGGTTATGGAAGAGATAGACGCGGGGNAGAATCGGTGGCGAGTAAGTTTCAGAACCGTT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,118 total letters Query= IDP95831.103 Length=1689 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2313 0.0 2_12B_B6_IDP95831.103_T7term.seq 1438 0.0 1_06B_B6_IDP95831.103_v73F.seq 930 0.0 W S H P Q F E K G T E N L Y F Q S N A M E K D P I P Q A T Query_1 145 TGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGT-ACTTCCAATCCAATGCCATGGAAAAAGATCCTATTCCTCAGGCCACGTC 233 0 1 ................................ 32 1 45 ...........A........G....------.........T................................................. 128 S P L A T W L S Y L E H L H S K T I D L G L A R V S E V A G Query_1 234 GCCCCTGGCTACGTGGCTTTCTTATCTGGAACATCTGCACAGTAAAACCATCGACCTTGGGCTGGCGCGCGTCAGCGAGGTCGCCGGGCA 323 0 33 .......................................................................................... 122 1 129 .........................................................G..........................GC.... 218 Q M D I V K P A P F V F T V A G T N G K G T T C R T L E T I Query_1 324 GATGGATATCGTTAAACCGGCGCCGTTCGTTTTTACCGTTGCGGGCACCAATGGCAAAGGCACCACCTGCCGCACGCTGGAAACCATCCT 413 0 123 .......................................................................................... 212 1 219 ............C.................C.....G..C.............................................G.... 308 L M A A G Y K V G V Y S S P H L V R Y T E R V R I Q G Query_1 414 GATGGCGGCCGGTTATAAAGTGGGGG--TCTACA-GCTCTCCG-CAT-CTGGTG-CGCTACACC--GAACGCGTGCGCATT-CAGGGG-- 492 0 213 ..........................--......-........-...-......-.........--...............-......-- 291 2 1146 .........GT.T...NN.N......N...T......G.NT......CGNNNG....NN.....T.....NAA 1074 1 309 ............C.............--......-........-...-......-..T......--...............-.....A-- 387 A E L P E A A H T A S F A E I E A A R G D I S L T Y F Query_1 493 GCTGAACTGCCGGAGG-CGGCGC-ATACAG--CTTCGTTTGCTGAA-ATCGAA-GCGGCGCGGGGT-GACATCTCGCTGACCTATTTTGA 575 0 292 ................-......-......--..............-......-............-....................... 374 2 1073 ..G.............A...N..C......GC........N.G..GG..T...A...........GC.................C..... 984 1 388 ..G.............-......-....G.--.C........G..G-..T...-...........C-.................C..... 470 E Y G T L S A L W L F K Q A Q L D V V I L E V G L G G R L Query_1 576 AT-ACGGCACGCTCTCGGCGCTGTGGCT-GTTT-AAGCAGG-CGCAGCTGGATGTGGTGAT-CCTTGAGGTCGGGCTCGGCGGGCGTCTC 660 0 375 ..-.........................-....-.......-...................-............................ 459 2 983 G.T.............T.....A...G.T....N.......G..........C........T...G.....G...........C...... 894 1 471 G.-.............T.....A...T.-....-.......-..........C........-...G.....G...........C...... 555 D A T N I V D A D V A V V T S I A L D H T D W L G P D R E Query_1 661 GACGCCACCAACATCGTGGATGCCGACGTCGCGGTGGTCACCAGCATCGCCCTCGATCATACCGACTGGCTGGGCCCGGATCGCGAAA-G 749 0 460 ........................................................................................-. 548 2 893 ..N.....T..T..............T.....C.....T...........T.......................A.............A. 804 1 556 ........T..T..............T.....C.....T....N......T.......................A.............-. 644 S I G R E K A G I F R A G K P A V V G E P D M P L T I A E V Query_1 750 TATCGGCCGGGA-AAAAGCGGGCATCTT-TCGCGCCGGTAAACCGGCGGTGGTGGGGGAGCCGGATATGCCGCTCACCATCGCGGAAGTG 837 0 549 ............-...............-............................................................. 636 2 803 ............-G.....C..T.....-C..T.....A...........A..A...................A......T..T...... 716 1 645 ......N.....G......C..T.....CC..T.....A..... 688 A S E K G A L L Q R R G V D W R Y E V E G E T W S F R D T Query_1 838 GCTAGCGAGAA-GGGCGCGCTGCTCCAGCGCCGCGGCGTCGACTGGCGCTATGAGGTGGAGGGCGAAACGTGGTCCTTCCGCGATACGGC 926 0 637 ...........-.............................................................................. 725 2 715 ..G........A......C.........................................A.....G..A.....T........CG...G 626 A G A L S H L P L P Q V P L P N A A T A V A A L R A S G L A Query_1 927 AGGGGCGTTAAGCCACCTGCCTCTGCCTCAGGTGCCGTTGCCGAATGCGGCGACGGCCGTGGCGGCGCTGCGCGCCAGCGGGCTTGCCGT 1016 0 726 .......................................................................................... 815 2 625 C............................................C..C..C.............................A........ 536 2 560 ..C..C............... 580 V D D A I L R A G I R D A M L P G R F Q I I S D A P R V I L Query_1 1017 GGATGACGCTATCCTGCGCGCAGGGATCCGCGATGCGATGCTCCCGGGACGCTTTCAGATAATCAGCGACGCCCCGCGAGTGATCCTCGA 1106 0 816 .......................................................................................... 905 0 839 ............ 828 2 535 .........A...........G...........C........G...................................G........... 446 D V A H N P H A A A Y L A G R L K T L A K T G R V L A V I G Query_1 1107 TGTGGCGCACAACCCGCACGCGGCGGCCTATCTCGCCGGGCGTCTCAAAACGTTAGCGAAAACGGGACGCGTGCTGGCGGTTATTGGTAT 1196 0 906 .......................................................................................... 995 2 445 .........T.................T.............................................................. 356 M L H D K D I A G T L A N L A P E V D A W Y C A P L E G P R Query_1 1197 GCTGCATGATAAGGATATCGCCGGCACGTTGGCCAATCTGGCCCCGGAAGTCGATGCCTGGTACTGCGCGCCGCTGGAAGGCCCGCGCGG 1286 0 996 .......................................................................................... 1085 2 355 ...............C..T...................................C..........................G........ 266 G A T A E Q L V E H L R C G T V Y S S V A Q A W R A A M A D Query_1 1287 CGCGACGGCAGAGCAGCTGGTGGAACATTTGCGCTGCGGTACAGTCTATAGCAGCGTGGCGCAGGCGTGGCGTGCGGCCATGGCTGACGC 1376 0 1086 .......................................................................................... 1175 2 265 G........G.......................TG....C...............................A.................. 176 A K V E D T V L V C G S F H T V A Q V M E E I D A G # N R W Query_1 1377 TAAAGTGGAAGATACCGTGCTGGTGTGTGGTTCGTTTCACACCGTAGCGCAGGTTATGGAAGAGATAGACGCGGGGNAGAATCGGTGGCG 1466 0 1176 .......................................................................................... 1265 2 175 ..........................................G..C..A...........................-............. 87 2 775 ........... 785 1 674 ........... 664 R V S F R T V * H W K W I T D P N S S S V D K L A A A L E H Query_1 1467 AGTAAGTTTCAGAACCGTTTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcacca 1556 0 1266 ................... 1284 2 86 .......................................--..... 43 H H H H H * D P A A N K A Query_1 1557 ccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCC 1594 2 47 ..................... 27 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5136110 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 3,118 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95851.105 (IDP95851.49-269.P(399)..pMCSG73)
NGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVVLQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGDQIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGNITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGL
AACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 1,189 total letters Query= IDP95851.105 Length=1068 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1196 0.0 2_12G_G6_IDP95851.105_T7term.seq 834 0.0 N G F E R E L Q N N I L G L M P Q A I L S S E H G S L N P Q Query_1 202 AACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAG 291 0 1 .......................................................................................... 90 Q L P E T A V K L D G V N R V A P I T T G D V V L Q S A R S Query_1 292 CAACTCCCAGAAACGGCAGTCAAACTGGACGGCGTTAATCGCGTCGCACCTATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGC 381 0 91 .......................................................................................... 180 0 319 ............. 331 2 389 ............. 377 V A V G V M L G I D P A Q K D P L T P Y L V N V K Q T D L E Query_1 382 GTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAG 471 0 181 .......................................................................................... 270 2 521 ..............N...........................N....N.N....A.................N........... 438 P G K Y N V I L G E Q L A S Q L G V N R G D Q I R V M V P S Query_1 472 CCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCT 561 0 271 .......................................................................................... 360 0 121 ............. 133 0 359 ............ 348 2 437 ..........................N..................N................N.............N............. 348 2 349 ............ 360 A S Q F T P M G R I P S Q R L F N V I G T F A A N S E V D G Query_1 562 GCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGC 651 0 361 .......................................................................................... 450 2 347 ...................................T...................................................... 258 Y E M L V N I E D A S R L M R Y P A G N I T G W R L W L D E Query_1 652 TATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAG 741 0 451 .......................................................................................... 540 2 257 A..............................N....N..................................................... 168 P L K V D S L S Q Q K L P E G S K W Q D W R D R K G E L F Q Query_1 742 CCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAG 831 0 541 .......................................................................................... 630 2 167 .....................................................................N................N... 78 A V R M E K N M M G L * H W K W I Query_1 832 GCCGTACGCATGGaaaaaaaTATGATGGGTTTATAACATTGGAAGTGGATAA 883 0 631 ................................. 663 2 77 .................................................... 26 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1208938 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 1,189 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95852.102 (IDP95852.1-422.F..pMCSG68)
MENKSIPQATSPLAAWLSYLENLHSKTIDMGLERVSQVAARLDVLKPAPFVFTVAGTNGKGTTCRTLESVLMAAGYKVGVYSSPHLVRYTERVRVQNTELAESAHTASFAAIEAARGETSLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNMVDADVAVVTSIALDHTDWLGPDRESIGREKAGIFRANKPAVVGEPDMPHTIAEVAKEKGARLLRRGVDWQYEVQDNGWRFSDAQGVLESLPLPQVPQPNAATALAALRASGLAVSEQAIRDGIQNALLPGRFQIVSESPRLILDVAHNPHAAAYLAGRLKSLPKTGRVLAVIGMLHDKDIGGTLACMESVVDSWYCAPLEGPRGATAEQLMEHLGKGEIYSSVVSAWRAAMAEAKPEDTVLVCGSFHTVAHVMEVMDAGRTGGE
ATGGAAAATAAAAGCATTCCTCAAGCCACGTCGCCCCTGGCCGCGTGGCTTTCTTATCTGGAAAACCTGCACAGTAAAACTATCGACATGGGCCTTGAGCGCGTAAGCCAGGTTGCCGCGCGTCTTGACGTGCTGAAACCCGCGCCTTTCGTGTTTACCGTCGCCGGCACTAACGGTAAAGGCACCACCTGCCGCACGCTGGAATCTGTCCTGATGGCGGCGGGGTACAAAGTTGGCGTTTACAGCTCGCCGCATCTGGTGCGCTATACCGAGCGCGTGCGGGTGCAAAACACCGAGCTGGCGGAATCGGCCCATACGGCGTCGTTTGCCGCTATCGAAGCGGCGCGCGGCGAAACCTCATTAACCTATTTCGAATATGGCACCCTGTCGGCGCTGTGGCTGTTTAAGCAGGCGCAGCTGGACGTGGTGATCCTGGAAGTGGGGCTGGGCGGGCGTCTGGATGCCACCAATATGGTGGATGCGGATGTCGCCGTCGTCACCAGCATCGCGCTGGATCACACCGACTGGCTGGGCCCGGACCGCGAGAGCATTGGCCGTGAGAAGGCCGGTATTTTCCGGGCCAATAAGCCTGCCGTGGTAGGCGAACCGGACATGCCACATACCATCGCTGAGGTAGCGAAAGAGAAGGGCGCTCGTCTGCTGCGTCGCGGCGTGGACTGGCAGTATGAGGTGCAGGACAACGGCTGGCGCTTCAGCGATGCGCAGGGCGTGCTGGAGAGTCTGCCCCTGCCGCAGGTGCCACAGCCTAACGCGGCGACTGCGCTGGCAGCCCTGCGCGCCAGCGGATTAGCGGTAAGCGAGCAGGCAATCCGCGATGGCATTCAGAACGCACTGTTACCAGGACGTTTTCAGATTGTGAGTGAGTCACCGCGTCTGATTCTGGATGTTGCACATAACCCGCATGCGGCGGCGTACCTCGCAGGACGTCTCAAATCGTTACCAAAAACCGGGCGCGTGCTGGCGGTTATCGGTATGCTTCATGATAAAGATATCGGCGGCACGCTGGCCTGCATGGAGAGTGTGGTCGATAGCTGGTATTGTGCTCCTCTGGAAGGGCCGCGCGGCGCGACTGCTGAGCAGTTGATGGAACATCTCGGCAAAGGCGAAATCTACAGCAGTGTGGTTTCGGCCTGGCGTGCCGCGATGGCGGAGGCTAAACCAGAAGATACCGTGCTGGTGTGTGGGTCATTCCACACGGTGGCACATGTCATGGAAGTGATGGACGCGGGGAGAACCGGTGGCGAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,497 total letters Query= IDP95852.102 Length=1671 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2284 0.0 2_07A_A1_IDP95852.102_T7term.seq 1236 0.0 1_01A_A1_IDP95852.102_T7sh.seq 1191 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 60 CCCCTCTAGAAATAA-TTTTGTTTAACTTT-AAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGG 147 1 24 ...T...........A..............T........................................................... 113 S H P Q F E K G T E N L Y F Q S N A M E N K S I P Q A T S P Query_1 148 TCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGGAAAATAAAAGCATTCCTCAAGCCACGTCGCCC 237 0 1 .................................... 36 1 114 .......................................................................................... 203 L A A W L S Y L E N L H S K T I D M G L E R V S Q V A A R L Query_1 238 CTGGCCGCGTGGCTTTCTTATCTGGAAAACCTGCACAGTAAAACTATCGACATGGGCCTTGAGCGCGTAAGCCAGGTTGCCGCGCGTCTT 327 0 37 .......................................................................................... 126 2 463 ........... 453 1 204 ......A...................G.................C..T..T.....G.....A.................T........C 293 D V L K P A P F V F T V A G T N G K G T T C R T L E S V L M Query_1 328 GACGTGCTGAAACCCGCGCCTTTCGTGTTTACCGTCGCCGGCACTAACGGTAAAGGCACCACCTGCCGCACGCTGGAATCTGTCCTGATG 417 0 127 .......................................................................................... 216 1 294 ..T...........T.....G..T..T..............A..C.......................T.........G........... 383 A A G Y K V G V Y S S P H L V R Y T E R V R V Q N T E L A E Query_1 418 GCGGCGGGGTACAAAGTTGGCGTTTACAGCTCGCCGCATCTGGTGCGCTATACCGAGCGCGTGCGGGTGCAAAACACCGAGCTGGCGGAA 507 0 217 .......................................................................................... 306 1 384 ..T..C..T.....G.....T..C........T.....C.....A..T...........G..T..C..........G...A...C.A..G 473 S A H T A S F A A I E A A R G E T S L T Y F E Y G T L S A Query_1 508 TCGGCCCATACGGCGTCGTTTGCCGCTATCGAAGCGGCGCGCGGCGAAACCTCATTAACCTATTT--CGAATATG-GCACCCTGTCGGCG 594 0 307 .................................................................--........-.............. 393 2 1055 .........N...TTN.....C.N...........A.A 1018 1 474 ..T..G..............C..G.AA..T..............T..G.T...............--......C.-.............A 560 L W L F K Q A Q L D V V I L E V G L G G R L D A T N M V Query_1 595 CTGTGGCTGTTTAAGC-AGGCGCAGCTGGACGTGGTGATCCTGGAAGTGGGGCT-GGGCGGGC-GTCTGGATGCCACCAATATG-GTGGA 680 0 394 ................-.....................................-........-....................-..... 479 2 1017 .N............A.C.......A..............T...........N..CN.......C..TCT..N............A..... 928 1 561 ..............A.-.......A..............T...........C..-C.......-....................-..... 646 D A D V A V V T S I A L D H T D W L G P D R E S I G R E K Query_1 681 TGCGGATGTCGCCGTCGTC-ACCAGCATCGCGCTGGATCACACCGACTGG-CTGGGCCCGGACCGCGAGA-GCATTGGCCGTGAGAA-GG 766 0 480 ...................-..............................-...................-................-.. 565 2 927 ...C.....G..G..GN..N.....T..T..C........T.........GNN......C..T.....A.A.T..............N.. 838 1 647 ...C.....G..G..G...-.....T..T..C........T.........-........C..T.....A.-.T..............-.. 732 A G I F R A N K P A V V G E P D M P H T I A E V A K E K G A Query_1 767 CCGGTATTTTCCGGGCCAATAAGCCTGCCGTGGTAGGCGAACCGGACATGCCACATACCATCGCTGAGGTAGCGAAAGAGAAGGGCGCTC 856 0 566 .......................................................................................... 655 2 837 .............CA.G........C..G.....N.....G.....T........C.....T..C..C..G................... 748 1 733 .............CA.G........C..G.....T.....G.....T........C.....T..C..C..G...........-....... 821 R L L R R G V D W Q Y E V Q D N G W R F S D A Q G V L E S L Query_1 857 GTCTGCTGCGTCGCGGCGTGGACTGGCAGTATGAGGTGCAGGACAACGGCTGGCGCTTCAGCGATGCGCAGGGC-GTGCTGGAGAGTCTG 945 0 656 ..........................................................................-............... 744 2 747 AC........C..G..T....................T...A...............ATC.............A-AC........A.... 659 1 822 AC........C..G..T........A...........-...A..T............ATC.............NAAC......N.A.... 910 P L P Q V P Q P N A A T A L A A L R A S G L A V S E Q A I R Query_1 946 CCCCTGCCGCAGGTGCCACAGCCTAACGCGGCGACTGCGCTGGCAGCCCTGCGCGCCAGCGGATTAGCGGTAAGCGAGCAGGCAATCCGC 1035 0 745 .......................................................................................... 834 2 658 ..............A..G..A.....T..C..C..C..C.....G..A........A..T..GC.G.....G.....A.....C...... 569 1 911 ..G...........A..G..A.....T.GC..C..C..-...TGC.TA........A..T..NC.GCN...G.....A.....C...... 999 D G I Q N A L L P G R F Q I V S E S P R L I L D V A H N P Query_1 1036 GATGG-CATTCAGAACGCACTGTTACCAGGACGTTTTCAGATTGTGAGTGAGTCACCGCGTCTGATTCTGGATGTTGCACATAACCCGCA 1124 0 835 .....-.................................................................................... 923 2 568 ..C..-T........T..GA.TC.G..C..G..............C..C..CG.T.....G.....A........C.............. 480 1 1000 ..C..TT...... 1012 H A A A Y L A G R L K S L P K T G R V L A V I G M L H D K D Query_1 1125 TGCGGCGGCGTACCTCGCAGGACGTCTCAAATCGTTACCAAAAACCGGGCGCGTGCTGGCGGTTATCGGTATGCTTCATGATAAAGATAT 1214 0 924 .......................................................................................... 1013 2 479 ...T........T.....C.CG.................................................................... 390 I G G T L A C M E S V V D S W Y C A P L E G P R G A T A E Q Query_1 1215 CGGCGGCACGCTGGCCTGCATGGAGAGTGTGGTCGATAGCTGGTATTGTGCTCCTCTGGAAGGGCCGCGCGGCGCGACTGCTGAGCAGTT 1304 0 1014 .......................................................................................... 1103 2 389 ......T.....................................................G...........T.....G..C........ 300 L M E H L G K G E I Y S S V V S A W R A A M A E A K P E D T Query_1 1305 GATGGAACATCTCGGCAAAGGCGAAATCTACAGCAGTGTGGTTTCGGCCTGGCGTGCCGCGATGGCGGAGGCTAAACCAGAAGATACCGT 1394 0 1104 .......................................................................................... 1193 2 299 AC.....................C........AT..C....C.CA...A....AA..............T.................... 210 V L V C G S F H T V A H V M E V M D A G R T G G E * H W K W Query_1 1395 GCTGGTGTGTGGGTCATTCCACACGGTGGCACATGTCATGGAAGTGATGGACGCGGGGAGAACCGGTGGCGAGTAACATTGGAAGTGGAT 1484 0 1194 ......................................................................... 1266 2 209 ............T..G.......................................................................... 120 I T D P N S S S V D K L A A A L E H H H H H H * D P A A N K Query_1 1485 AACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCT-AACAAAG 1573 2 119 ..................................................................................A....... 30 A Query_1 1574 CCC 1576 2 29 N.. 27 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5708095 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 3,497 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95866.102 (IDP95866.1-307.F..pMCSG68)
MSMADRDGVIWYDGELVQWRDATTHVLTHTLHYGMGVFEGVRAYDTPQGTAIFRLQAHTDRLFDSAHIMNMQIPYSRDEINEATAPPCARTTWKAPISARWCSTGSEGMGLRASGLKVHVIIASWSWGAYMGEEALQQGIKVRTSSFTRHHVNISMTRAKSNGAYINSMLALQEAISGGADEAMMLDPEGYVAEGSGENIFIIKDGVIYTPEVTACLNGITRNTILTLAAEHGFKLVEKRITRDEVYIADEAFFTGTAAEVTPIREVDGRKIGAGRRGPVTEKLQKAYFDLVSGKTEAHAEWRTLVK
ATGTCGATGGCCGATCGTGATGGCGTGATCTGGTATGACGGTGAACTGGTGCAGTGGCGCGACGCGACCACGCACGTGCTGACCCATACCCTGCACTATGGAATGGGCGTGTTCGAGGGCGTGCGCGCCTACGACACCCCGCAGGGCACGGCGATCTTCCGCCTGCAGGCGCATACCGACCGGCTGTTCGACTCCGCGCACATCATGAACATGCAGATCCCGTACAGCCGCGACGAGATCAACGAGGCGACCGCGCCGCCGTGCGCGAGAACAACCTGGAAAGCGCCTATATCCGCCCGATGGTGTTCTACGGGAAGCGAAGGCATGGGCCTGCGCGCCAGCGGCCTGAAGGTCCATGTGATCATCGCCTCCTGGAGCTGGGGCGCCTACATGGGCGAGGAAGCCCTGCAGCAAGGCATCAAGGTGCGCACCAGTTCCTTCACCCGCCACCACGTCAACATCTCGATGACCCGTGCCAAGTCCAACGGCGCCTACATCAACTCGATGCTGGCCCTCCAGGAAGCGATCTCCGGCGGCGCCGACGAGGCCATGATGCTCGATCCGGAAGGCTACGTGGCCGAAGGCTCCGGCGAGAACATCTTCATCATCAAGGATGGCGTGATCTACACCCCGGAAGTCACCGCCTGCCTGAACGGCATCACTCGTAACACTATCCTGACCCTGGCCGCCGAACACGGTTTTAAACTGGTCGAGAAGCGCATCACCCGCGACGAGGTGTACATCGCCGACGAGGCCTTCTTCACTGGCACTGCCGCGGAAGTCACGCCGATCCGCGAAGTGGACGGTCGCAAGATCGGCGCCGGCCGCCGTGGCCCGGTCACCGAAAAGCTGCAGAAAGCCTATTTCGACCTGGTCAGCGGCAAGACCGAGGCCCACGCCGAGTGGCGTACCCTGGTCAAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 1,414 total letters Query= IDP95866.102 Length=1326 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1662 0.0 1_01F_F1_IDP95866.102_T7sh.seq 765 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 60 CCCCTCTAGAAATAATTTTGTTTAACTTT-AAGAAGGAGATATACATATGCACCATCAT-CATCATCATTCTTCTGGTGTAGATCTGTGG 147 1 21 .......NN..TN.T..............T.............................T.............................. 110 S H P Q F E K G T E N L Y F Q S N A M S M A D R D G V I W Y Query_1 148 TCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGTCGATGGCCGATCGTGATGGCGTGATCTGGTAT 237 0 1 .................................... 36 0 613 ............. 625 1 111 .......................................................................................... 200 D G E L V Q W R D A T T H V L T H T L H Y G M G V F E G V R Query_1 238 GACGGTGAACTGGTGCAGTGGCGCGACGCGACCACGCACGTGCTGACCCATACCCTGCACTATGGAATGGGCGTGTTCGAGGGCGTGCGC 327 0 37 .......................................................................................... 126 1 201 ...........................N..........................A................................... 290 A Y D T P Q G T A I F R L Q A H T D R L F D S A H I M N M Q Query_1 328 GCCTACGACACCCCGCAGGGCACGGCGATCTTCCGCCTGCAGGCGCATACCGACCGGCTGTTCGACTCCGCGCACATCATGAACATGCAG 417 0 127 .......................................................................................... 216 0 171 ............ 160 1 291 ....................N...................................................................N. 380 1 335 ............ 324 I P Y S R D E I N E A T A P P C A R T T W K A P I S A R W Query_1 418 ATCCCGTACAGCCGCGACGAGATCAACGAGGCGA-CCGCGCCGCCGTGCGCGAGAACAACCTGGAAAGCGCCTATATCCGCCCGATGGTG 506 0 217 ..................................-....................................................... 305 1 381 ..................................C.....................T........N.............N.......... 470 C S T G S E G M G L R A S G L K V H V I I A S W S W G A Y M Query_1 507 TTCTACGGGAAGCGAAGGCATGGGCCTGCGCGCCAGCGGCCTGAAGGTCCATGTGATCATCGCCTCCTGGAGCTGGGGCGCCTACATGGG 596 0 306 .......................................................................................... 395 0 487 ........... 497 1 471 ......-........... 487 G E E A L Q Q G I K V R T S S F T R H H V N I S M T R A K S Query_1 597 CGAGGAAGCCCTGCAGCAAGGCATCAAGGTGCGCACCAGTTCCTTCACCCGCCACCACGTCAACATCTCGATGACCCGTGCCAAGTCCAA 686 0 396 .......................................................................................... 485 N G A Y I N S M L A L Q E A I S G G A D E A M M L D P E G Y Query_1 687 CGGCGCCTACATCAACTCGATGCTGGCCCTCCAGGAAGCGATCTCCGGCGGCGCCGACGAGGCCATGATGCTCGATCCGGAAGGCTACGT 776 0 486 .......................................................................................... 575 0 744 ............. 756 0 382 ........... 392 V A E G S G E N I F I I K D G V I Y T P E V T A C L N G I T Query_1 777 GGCCGAAGGCTCCGGCGAGAACATCTTCATCATCAAGGATGGCGTGATCTACACCCCGGAAGTCACCGCCTGCCTGAACGGCATCACTCG 866 0 576 .......................................................................................... 665 0 19 ............. 31 1 183 ............. 195 R N T I L T L A A E H G F K L V E K R I T R D E V Y I A D E Query_1 867 TAACACTATCCTGACCCTGGCCGCCGAACACGGTTTTAAACTGGTCGAGAAGCGCATCACCCGCGACGAGGTGTACATCGCCGACGAGGC 956 0 666 .......................................................................................... 755 0 537 ............ 548 A F F T G T A A E V T P I R E V D G R K I G A G R R G P V T Query_1 957 CTTCTTCACTGGCACTGCCGCGGAAGTCACGCCGATCCGCGAAGTGGACGGTCGCAAGATCGGCGCCGGCCGCCGTGGCCCGGTCACCGA 1046 0 756 .......................................................................................... 845 0 549 . 549 E K L Q K A Y F D L V S G K T E A H A E W R T L V K Query_1 1047 AAAGCTGCAGAAAGCCTATTTCGACCTGGTCAGCGGCAAGACCGAGGCCCACGCCGAGTGGCGTACCCTGGTCAAG 1122 0 846 ............................................................................ 921 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1794759 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 1,414 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95866.103 (IDP95866.1-307.F..pMCSG73)
MSMADRDGVIWYDGELVQWRDATTHVLTHTLHYGMGVFEGVRAYDTPQGTAIFRLQAHTDRLFDSAHIMNMQIPYSRDEINEATAPPCARTTWKAPISARWCSTGSEGMGLRASGLKVHVIIASWSWGAYMGEEALQQGIKVRTSSFTRHHVNISMTRAKSNGAYINSMLALQEAISGGADEAMMLDPEGYVAEGSGENIFIIKDGVIYTPEVTACLNGITRNTILTLAAEHGFKLVEKRITRDEVYIADEAFFTGTAAEVTPIREVDGRKIGAGRRGPVTEKLQKAYFDLVSGKTEAHAEWRTLVK
ATGTCGATGGCCGATCGTGATGGCGTGATCTGGTATGACGGTGAACTGGTGCAGTGGCGCGACGCGACCACGCACGTGCTGACCCATACCCTGCACTATGGAATGGGCGTGTTCGAGGGCGTGCGCGCCTACGACACCCCGCAGGGCACGGCGATCTTCCGCCTGCAGGCGCATACCGACCGGCTGTTCGACTCCGCGCACATCATGAACATGCAGATCCCGTACAGCCGCGACGAGATCAACGAGGCGACCGCGCCGCCGTGCGCGAGAACAACCTGGAAAGCGCCTATATCCGCCCGATGGTGTTCTACGGGAAGCGAAGGCATGGGCCTGCGCGCCAGCGGCCTGAAGGTCCATGTGATCATCGCCTCCTGGAGCTGGGGCGCCTACATGGGCGAGGAAGCCCTGCAGCAAGGCATCAAGGTGCGCACCAGTTCCTTCACCCGCCACCACGTCAACATCTCGATGACCCGTGCCAAGTCCAACGGCGCCTACATCAACTCGATGCTGGCCCTCCAGGAAGCGATCTCCGGCGGCGCCGACGAGGCCATGATGCTCGATCCGGAAGGCTACGTGGCCGAAGGCTCCGGCGAGAACATCTTCATCATCAAGGATGGCGTGATCTACACCCCGGAAGTCACCGCCTGCCTGAACGGCATCACTCGTAACACTATCCTGACCCTGGCCGCCGAACACGGTTTTAAACTGGTCGAGAAGCGCATCACCCGCGACGAGGTGTACATCGCCGACGAGGCCTTCTTCACTGGCACTGCCGCGGAAGTCACGCCGATCCGCGAAGTGGACGGTCGCAAGATCGGCGCCGGCCGCCGTGGCCCGGTCACCGAAAAGCTGCAGAAAGCCTATTTCGACCTGGTCAGCGGCAAGACCGAGGCCCACGCCGAGTGGCGTACCCTGGTCAAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,463 total letters Query= IDP95866.103 Length=1326 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1662 0.0 2_12H_H6_IDP95866.103_T7term.seq 1588 0.0 1_06H_H6_IDP95866.103_v73F.seq 646 0.0 N L Y F Q S N A M S M A D R D G V I W Y D G E L V Q Query_1 176 AGAACCTG--TA-CTTCCAAT-CCAATGCCATGT-CGAT-GGCCGATCGTGAT-GGCGTGATCTGGTATGACGG-TGAACTGGTGCAGTG 257 0 1 ....-....-.............-....................-............... 56 0 613 ...-.......... 625 2 1023 ....N.--..N........-.........N..N.N..-.A.....N.....T....................G....N.......... 940 2 378 ...-.......... 366 1 17 .......TGT..-........T..N.........T....T.............-..N.................-............... 103 W R D A T T H V L T H T L H Y G M G V F E G V R A Y D T P Query_1 258 GCGCGACGCGACCACGCACGT-GCTGACCCAT-ACCCTGCACTATGGAATGGG-CGTGTTCGA-GGGCGTGCGCGCCTACGACACCCCGC 343 0 57 .....................-..........-....................-.........-.......................... 142 2 939 .....................T.N........T............N.......G......N..A............TA.........-.. 851 1 104 .N.....N.............-........-.-....................-N........-.......................... 188 Q G T A I F R L Q A H T D R L F D S A H I M N M Q I P Y S R Query_1 344 AGGGCACGGCGATCTTCCGCCTGCAGGCGCATACCGACCGGCTGTTCGACTCCGCGCACATCATGAACATGCAGATCCCGTACAGCCGCG 433 0 143 .......................................................................................... 232 0 171 ............ 160 2 850 ...........................N........T..N....G............................................. 761 2 824 .......... 833 1 189 .........................N...............N...............................................N 278 1 217 ...N........ 206 D E I N E A T A P P C A R T T W K A P I S A R W C S T G S Query_1 434 ACGAGATCAACGAGG-CGA-CC-GCGCCGCCGTGCGCGAGAACAACCTGGAAA-GCGCCTATATCCGCCCGATGGTGTTCTACGGGAAGC 519 0 233 ...............-...-..-..............................-.................................... 318 2 760 ...............G...-..C..............................A...............................-.... 673 1 279 ...............-...C..-..........................N...-.............................-T..... 364 E G M G L R A S G L K V H V I I A S W S W G A Y M G E E A L Query_1 520 GAAGGCATGGGCCTGCGCGCCAGCGGCCTGAAGGTCCATGTGATCATCGCCTCCTGGAGCTGGGGCGCCTACATGGGCGAGGAAGCCCTG 609 0 319 .......................................................................................... 408 0 487 ........... 497 2 672 ...................................................G...................................... 583 2 504 ........... 494 1 365 ...N...............................................N......NN............N.. 439 Q Q G I K V R T S S F T R H H V N I S M T R A K S N G A Y I Query_1 610 CAGCAAGGCATCAAGGTGCGCACCAGTTCCTTCACCCGCCACCACGTCAACATCTCGATGACCCGTGCCAAGTCCAACGGCGCCTACATC 699 0 409 .......................................................................................... 498 0 382 ........... 392 2 582 .................................................................C........................ 493 2 609 ........... 599 N S M L A L Q E A I S G G A D E A M M L D P E G Y V A E G S Query_1 700 AACTCGATGCTGGCCCTCCAGGAAGCGATCTCCGGCGGCGCCGACGAGGCCATGATGCTCGATCCGGAAGGCTACGTGGCCGAAGGCTCC 789 0 499 .......................................................................................... 588 0 744 ............. 756 2 492 .......................................................................................... 403 2 247 ............. 235 G E N I F I I K D G V I Y T P E V T A C L N G I T R N T I L Query_1 790 GGCGAGAACATCTTCATCATCAAGGATGGCGTGATCTACACCCCGGAAGTCACCGCCTGCCTGAACGGCATCACTCGTAACACTATCCTG 879 0 589 .......................................................................................... 678 0 19 ............. 31 2 402 .......................................................................................... 313 2 976 ........... 966 T L A A E H G F K L V E K R I T R D E V Y I A D E A F F T G Query_1 880 ACCCTGGCCGCCGAACACGGTTTTAAACTGGTCGAGAAGCGCATCACCCGCGACGAGGTGTACATCGCCGACGAGGCCTTCTTCACTGGC 969 0 679 .......................................................................................... 768 0 537 ............. 549 2 312 .......................................................................................... 223 2 454 ............. 442 T A A E V T P I R E V D G R K I G A G R R G P V T E K L Q K Query_1 970 ACTGCCGCGGAAGTCACGCCGATCCGCGAAGTGGACGGTCGCAAGATCGGCGCCGGCCGCCGTGGCCCGGTCACCGAAAAGCTGCAGAAA 1059 0 769 .......................................................................................... 858 2 222 .......................................................................................... 133 A Y F D L V S G K T E A H A E W R T L V K * H W K W I T D P Query_1 1060 GCCTATTTCGACCTGGTCAGCGGCAAGACCGAGGCCCACGCCGAGTGGCGTACCCTGGTCAAGTAACATTGGAAGTGGATAACGGATCCG 1149 0 859 ............................................................... 921 2 132 ................................................................................... 50 N S S S V D K L A A A L E H H H H H H * D P A A N K A R K Query_1 1150 AATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCT-AACAAAGCCC--GAAAG 1236 2 48 ...........A..........CGA.... 20 E Query_1 1237 GAAG 1240 2 19 .... 16 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3163650 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 2,463 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95886.102 (IDP95886.1-159.F..pMCSG68)
MTLAYIAIGSNLASPLEQVNAAVQALGEIPQSKIVAVSSFYRTPPLGPQDQPDYLNAAVILETELGPEELLDNTQRIELQQGRVRKAERWGPRTLDLDIMLFGHETINTERLTVPHYDMKNRGFMLWPLFEVAPDLIFPDGTPLKSILDNLNAERPARW
ATGACCCTTGCGTATATCGCCATCGGCAGCAATTTAGCCTCTCCGCTGGAGCAGGTGAATGCTGCCGTACAGGCGCTGGGTGAGATCCCGCAAAGCAAGATTGTGGCTGTCTCCTCGTTCTACCGCACTCCGCCGTTGGGTCCGCAGGATCAGCCTGATTATCTGAACGCCGCCGTGATCCTTGAGACTGAACTGGGCCCAGAAGAGCTGCTGGACAATACCCAGCGCATCGAACTGCAGCAGGGCCGCGTTCGCAAAGCCGAGCGCTGGGGACCGCGCACGCTGGACCTCGACATTATGCTGTTTGGTCACGAGACCATTAACACTGAACGTCTCACCGTTCCGCATTACGACATGAAAAATCGCGGGTTTATGCTCTGGCCGCTGTTTGAAGTCGCCCCCGATCTTATCTTCCCGGATGGCACCCCACTTAAGTCCATTCTGGACAACCTCAACGCGGAAAGACCCGCTCGCTGG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,367 total letters Query= IDP95886.102 Length=882 Score E Sequences producing significant alignments: (Bits) Value 1_01B_B1_IDP95886.102_T7sh.seq 1076 0.0 0_wt 861 0.0 2_07B_B1_IDP95886.102_T7term.seq 809 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 60 CCCCTCT-AGAAATAATTTTGTTTAACTTTAA-GAAGGAGATATACATATGCACCATCATC-ATCATCATTCTTCTGGTGTAGATCTGTG 146 1 22 ......NN....N...................A............................-............................ 110 2 678 .....C..........G.N...N........... 645 W S H P Q F E K G T E N L Y F Q S N A M T L A Y I A I G S N Query_1 147 G-TCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGACCCTTGCGTATATCGCCATCGGCAGCAATT 235 1 111 .-.......................................................................................C 199 0 1 .................................. 34 2 644 .G...............................T..............N........................................C 555 L A S P L E Q V N A A V Q A L G E I P Q S K I V A V S S F Y Query_1 236 TAGCCTCTCCGCTGGAGCAGGTGAATGCTGCCGTACAGGCGCTGGGTGAGATCCCGCAAAGCAAGATTGTGGCTGTCTCCTCGTTCTACC 325 1 200 .G........T...........T...........T....................C..G...CGC........G...........T.... 289 0 35 .......................................................................................... 124 2 554 .G........TN..........T...........T....................C..G...CGC........G...........T.... 465 R T P P L G P Q D Q P D Y L N A A V I L E T E L G P E E L L Query_1 326 GCACTCCGCCGTTGGGTCCGCAGGATCAGCCTGATTATCTGAACGCCGCCGTGATCCTTGAGACTGAACTGGGCCCAGAAGAGCTGCTGG 415 1 290 .T..A......C.C..C....................C.....T.........G.G..G..C..C.CG....ATG.C...AC.T....T. 379 0 125 .......................................................................................... 214 2 464 .T..A......C.C..C....................CN....T.........G.G..G..C..C.CG....ATG.C...AC.T....T. 375 D N T Q R I E L Q Q G R V R K A E R W G P R T L D L D I M L Query_1 416 ACAATACCCAGCGCATCGAACTGCAGCAGGGCCGCGTTCGCAAAGCCGAGCGCTGGGGACCGCGCACGCTGGACCTCGACATTATGCTGT 505 1 380 .T..C...........T..G.....A.....T.....G..T.....A...........G..A..T..C..T...........C....... 469 0 215 .......................................................................................... 304 2 374 .T..C...........T..G.....A.....T.....G..T.....A...........G..A..T..C..T...........C....... 285 F G H E T I N T E R L T V P H Y D M K N R G F M L W P L F E Query_1 506 TTGGTCACGAGACCATTAACACTGAACGTCTCACCGTTCCGCATTACGACATGAAAAATCGCGGGTTTATGCTCTGGCCGCTGTTTGAAG 595 1 470 ....CA.....GTG.........TC...C..G..G..G..........................A......................... 559 0 305 .......................................................................................... 394 2 284 ....CA.....GTG.........TC...C..G..G..G..........................A......................... 195 V A P D L I F P D G T P L K S I L D N L N A E R P A R W * H Query_1 596 TCGCCCCCGATCTTATCTTCCCGGATGGCACCCCACTTAAGTCCATTCTGGACAACCTCAACGCGGAAAGACCCGCTCGCTGGTAACATT 685 1 560 .............C.C.........C....T...G..C.G.GAG..C............C....A......................... 649 0 395 ................................................................................... 477 2 194 .............C.C.........C....T...G..C.G.GAG..C............C....A......................... 105 W K W I T D P N S S S V D K L A A A L E H H H H H H * D P Query_1 686 GGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcacca-ccaccacca-ccacTGAGA-TCCGG 772 1 650 ........................................................N.......C..C......C.........-..... 738 2 104 ................................................................-.........-.........N..... 17 A A N K A R K E A E L A A A T A E Q * L A * P L G A S K R Query_1 773 CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAC-CCCTTGGGG-CCTC-TAAACGGG 859 1 739 ...........N...........N..........................................T.........C....T........ 828 V L R G F L L Query_1 860 TC-TTGAGGGGTTTTTTGCTGAA 881 1 829 ..T.................... 851 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2013174 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 2,367 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95892.106 (IDP95892.1-549.F..pMCSG73)
MFKHSIFLRIYAGLVILVVLVAVFGYLLVQIINYQRAQEYRESLTDGISYVISEGVARQPGKQQKIDWISDASDLLELPIYYTDASKVELSRTEKKRIEAQKSVVRYDASNSIAYVIIGLRDDPQHYLSIKVDKITERQMKALPIFVLDYLMFYPGQEQEYLAKIQKHFSYPINIQNIQDVNLDSEQIGRLRQDQSVMLYKDSATVRGTTISIVSPIPNHPAQVLVLGPVPMFNWMPLQLSAGITLFSLFLLSLGVYGLILPLERKIRQVRYALNRMKSGDLSLRVPIEGSDEMANLASSYNNMSDHIQRLIEAQRELMRAVSHELRTPVARIRFGTEMLAEEDDYNHRMHQVDMIDKDIEALNTLIDEIMTYAKLEQGTPSLDFAEIVLFEVLDQVAVETEALKTQKEIELIPPPLYVKVDAERRYLHRVVQNLVGNAVRYCDNKVRITGGIHNDGMAFVCVEDDGPGIPEQDRKRVFEAFARLDDSRTRASGGYGLGLSIVSRIAYWFGGEIKVDESPSLGGARFIMTWPAHRFKQPPLKTNKKAPA
GTGTTTAAACACAGTATATTCCTGCGAATATATGCGGGGCTGGTAATTCTTGTTGTTTTGGTAGCTGTTTTTGGGTATTTACTGGTACAAATTATCAACTACCAACGCGCACAAGAATACCGAGAATCTTTAACTGATGGTATTTCTTATGTCATTAGTGAAGGAGTCGCTCGACAACCTGGGAAGCAGCAAAAAATAGATTGGATTTCTGACGCATCTGATTTGCTCGAACTTCCAATTTATTATACCGATGCAAGTAAGGTTGAGCTGTCTCGGACCGAGAAAAAGCGGATAGAAGCACAAAAATCTGTAGTTCGTTACGATGCAAGCAATAGCATTGCATATGTCATTATTGGCTTACGTGACGACCCACAACATTACTTATCTATAAAAGTCGACAAAATTACTGAACGCCAAATGAAGGCTCTTCCTATTTTTGTGCTCGACTATTTAATGTTTTATCCGGGGCAGGAACAAGAATATCTTGCCAAGATTCAAAAGCATTTCTCATATCCGATCAATATTCAAAATATTCAAGATGTAAATCTGGATTCTGAACAGATTGGACGTTTGCGCCAAGACCAAAGTGTCATGTTGTACAAAGATAGCGCAACAGTGCGCGGTACAACCATTTCGATTGTGTCTCCAATACCGAATCATCCTGCGCAAGTGTTAGTGCTGGGTCCGGTTCCAATGTTTAACTGGATGCCTTTACAGCTTTCGGCGGGTATTACCTTATTTAGCTTATTCTTATTAAGTCTAGGTGTTTACGGTTTGATTTTGCCGTTAGAGCGCAAAATCCGACAGGTGCGTTATGCATTAAACCGTATGAAATCGGGTGATTTGTCACTGCGTGTTCCTATTGAGGGAAGCGATGAAATGGCAAACTTGGCTTCAAGTTATAACAATATGTCTGACCATATTCAGCGTTTGATTGAGGCTCAACGTGAGTTAATGAGAGCAGTATCTCATGAACTCAGAACACCTGTGGCACGTATTCGCTTTGGTACAGAAATGTTAGCCGAAGAAGATGATTACAATCATCGTATGCATCAGGTCGACATGATTGATAAAGATATTGAAGCGCTCAATACCTTAATTGATGAAATCATGACTTATGCAAAACTAGAGCAGGGTACACCTTCATTAGACTTTGCAGAAATTGTACTTTTTGAAGTGTTGGATCAGGTTGCAGTTGAAACTGAGGCTTTAAAAACACAGAAAGAAATTGAACTTATTCCACCGCCTTTATATGTGAAAGTAGATGCAGAGCGTCGTTACCTTCACCGTGTCGTGCAAAATCTAGTAGGTAATGCAGTTCGTTATTGTGATAACAAAGTTCGGATTACGGGCGGTATTCATAATGATGGCATGGCTTTTGTCTGTGTCGAAGACGACGGACCGGGTATTCCTGAGCAAGACCGCAAGCGTGTATTTGAAGCCTTTGCCCGTTTAGATGATAGCCGTACACGTGCATCTGGCGGTTATGGTTTGGGTCTCTCTATCGTAAGTCGTATTGCTTACTGGTTTGGTGGGGAAATTAAGGTAGATGAAAGTCCAAGTTTGGGAGGCGCACGCTTTATTATGACTTGGCCAGCACATCGCTTTAAACAACCGCCATTAAAGACCAATAAAAAAGCACCTGCA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 1,657 total letters Query= IDP95892.106 Length=2052 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2971 0.0 V F K H S I F L R I Y A G L V I L V V L V A V F G Y L L V Q Query_1 202 GTGTTTAAACACAGTATATTCCTGCGAATATATGCGGGGCTGGTAATTCTTGTTGTTTTGGTAGCTGTTTTTGGGTATTTACTGGTACAA 291 0 1 .......................................................................................... 90 0 12 ............ 1 I I N Y Q R A Q E Y R E S L T D G I S Y V I S E G V A R Q P Query_1 292 ATTATCAACTACCAACGCGCACAAGAATACCGAGAATCTTTAACTGATGGTATTTCTTATGTCATTAGTGAAGGAGTCGCTCGACAACCT 381 0 91 .......................................................................................... 180 G K Q Q K I D W I S D A S D L L E L P I Y Y T D A S K V E L Query_1 382 GGGAAGCAGCAAAAAATAGATTGGATTTCTGACGCATCTGATTTGCTCGAACTTCCAATTTATTATACCGATGCAAGTAAGGTTGAGCTG 471 0 181 .......................................................................................... 270 S R T E K K R I E A Q K S V V R Y D A S N S I A Y V I I G L Query_1 472 TCTCGGACCGAGAAAAAGCGGATAGAAGCACAAAAATCTGTAGTTCGTTACGATGCAAGCAATAGCATTGCATATGTCATTATTGGCTTA 561 0 271 .......................................................................................... 360 0 443 ........... 433 R D D P Q H Y L S I K V D K I T E R Q M K A L P I F V L D Y Query_1 562 CGTGACGACCCACAACATTACTTATCTATAAAAGTCGACAAAATTACTGAACGCCAAATGAAGGCTCTTCCTATTTTTGTGCTCGACTAT 651 0 361 .......................................................................................... 450 0 307 ........... 297 L M F Y P G Q E Q E Y L A K I Q K H F S Y P I N I Q N I Q D Query_1 652 TTAATGTTTTATCCGGGGCAGGAACAAGAATATCTTGCCAAGATTCAAAAGCATTTCTCATATCCGATCAATATTCAAAATATTCAAGAT 741 0 451 .......................................................................................... 540 V N L D S E Q I G R L R Q D Q S V M L Y K D S A T V R G T T Query_1 742 GTAAATCTGGATTCTGAACAGATTGGACGTTTGCGCCAAGACCAAAGTGTCATGTTGTACAAAGATAGCGCAACAGTGCGCGGTACAACC 831 0 541 .......................................................................................... 630 I S I V S P I P N H P A Q V L V L G P V P M F N W M P L Q L Query_1 832 ATTTCGATTGTGTCTCCAATACCGAATCATCCTGCGCAAGTGTTAGTGCTGGGTCCGGTTCCAATGTTTAACTGGATGCCTTTACAGCTT 921 0 631 .......................................................................................... 720 S A G I T L F S L F L L S L G V Y G L I L P L E R K I R Q V Query_1 922 TCGGCGGGTATTACCTTATTTAGCTTATTCTTATTAAGTCTAGGTGTTTACGGTTTGATTTTGCCGTTAGAGCGCAAAATCCGACAGGTG 1011 0 721 .......................................................................................... 810 R Y A L N R M K S G D L S L R V P I E G S D E M A N L A S S Query_1 1012 CGTTATGCATTAAACCGTATGAAATCGGGTGATTTGTCACTGCGTGTTCCTATTGAGGGAAGCGATGAAATGGCAAACTTGGCTTCAAGT 1101 0 811 .......................................................................................... 900 Y N N M S D H I Q R L I E A Q R E L M R A V S H E L R T P V Query_1 1102 TATAACAATATGTCTGACCATATTCAGCGTTTGATTGAGGCTCAACGTGAGTTAATGAGAGCAGTATCTCATGAACTCAGAACACCTGTG 1191 0 901 .......................................................................................... 990 A R I R F G T E M L A E E D D Y N H R M H Q V D M I D K D I Query_1 1192 GCACGTATTCGCTTTGGTACAGAAATGTTAGCCGAAGAAGATGATTACAATCATCGTATGCATCAGGTCGACATGATTGATAAAGATATT 1281 0 991 .......................................................................................... 1080 E A L N T L I D E I M T Y A K L E Q G T P S L D F A E I V L Query_1 1282 GAAGCGCTCAATACCTTAATTGATGAAATCATGACTTATGCAAAACTAGAGCAGGGTACACCTTCATTAGACTTTGCAGAAATTGTACTT 1371 0 1081 .......................................................................................... 1170 F E V L D Q V A V E T E A L K T Q K E I E L I P P P L Y V K Query_1 1372 TTTGAAGTGTTGGATCAGGTTGCAGTTGAAACTGAGGCTTTAAAAACACAGAAAGAAATTGAACTTATTCCACCGCCTTTATATGTGAAA 1461 0 1171 .......................................................................................... 1260 V D A E R R Y L H R V V Q N L V G N A V R Y C D N K V R I T Query_1 1462 GTAGATGCAGAGCGTCGTTACCTTCACCGTGTCGTGCAAAATCTAGTAGGTAATGCAGTTCGTTATTGTGATAACAAAGTTCGGATTACG 1551 0 1261 .......................................................................................... 1350 G G I H N D G M A F V C V E D D G P G I P E Q D R K R V F E Query_1 1552 GGCGGTATTCATAATGATGGCATGGCTTTTGTCTGTGTCGAAGACGACGGACCGGGTATTCCTGAGCAAGACCGCAAGCGTGTATTTGAA 1641 0 1351 .......................................................................................... 1440 A F A R L D D S R T R A S G G Y G L G L S I V S R I A Y W F Query_1 1642 GCCTTTGCCCGTTTAGATGATAGCCGTACACGTGCATCTGGCGGTTATGGTTTGGGTCTCTCTATCGTAAGTCGTATTGCTTACTGGTTT 1731 0 1441 .......................................................................................... 1530 G G E I K V D E S P S L G G A R F I M T W P A H R F K Q P P Query_1 1732 GGTGGGGAAATTAAGGTAGATGAAAGTCCAAGTTTGGGAGGCGCACGCTTTATTATGACTTGGCCAGCACATCGCTTTAAACAACCGCCA 1821 0 1531 .......................................................................................... 1620 L K T N K K A P A Query_1 1822 TTAAAGACCAATAAAAAAGCACCTGCA 1848 0 1621 ........................... 1647 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3275924 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 1,657 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95892.107 (IDP95892.30-549.P(549)..pMCSG73)
QIINYQRAQEYRESLTDGISYVISEGVARQPGKQQKIDWISDASDLLELPIYYTDASKVELSRTEKKRIEAQKSVVRYDASNSIAYVIIGLRDDPQHYLSIKVDKITERQMKALPIFVLDYLMFYPGQEQEYLAKIQKHFSYPINIQNIQDVNLDSEQIGRLRQDQSVMLYKDSATVRGTTISIVSPIPNHPAQVLVLGPVPMFNWMPLQLSAGITLFSLFLLSLGVYGLILPLERKIRQVRYALNRMKSGDLSLRVPIEGSDEMANLASSYNNMSDHIQRLIEAQRELMRAVSHELRTPVARIRFGTEMLAEEDDYNHRMHQVDMIDKDIEALNTLIDEIMTYAKLEQGTPSLDFAEIVLFEVLDQVAVETEALKTQKEIELIPPPLYVKVDAERRYLHRVVQNLVGNAVRYCDNKVRITGGIHNDGMAFVCVEDDGPGIPEQDRKRVFEAFARLDDSRTRASGGYGLGLSIVSRIAYWFGGEIKVDESPSLGGARFIMTWPAHRFKQPPLKTNKKAPA
CAAATTATCAACTACCAACGCGCACAAGAATACCGAGAATCTTTAACTGATGGTATTTCTTATGTCATTAGTGAAGGAGTCGCTCGACAACCTGGGAAGCAGCAAAAAATAGATTGGATTTCTGACGCATCTGATTTGCTCGAACTTCCAATTTATTATACCGATGCAAGTAAGGTTGAGCTGTCTCGGACCGAGAAAAAGCGGATAGAAGCACAAAAATCTGTAGTTCGTTACGATGCAAGCAATAGCATTGCATATGTCATTATTGGCTTACGTGACGACCCACAACATTACTTATCTATAAAAGTCGACAAAATTACTGAACGCCAAATGAAGGCTCTTCCTATTTTTGTGCTCGACTATTTAATGTTTTATCCGGGGCAGGAACAAGAATATCTTGCCAAGATTCAAAAGCATTTCTCATATCCGATCAATATTCAAAATATTCAAGATGTAAATCTGGATTCTGAACAGATTGGACGTTTGCGCCAAGACCAAAGTGTCATGTTGTACAAAGATAGCGCAACAGTGCGCGGTACAACCATTTCGATTGTGTCTCCAATACCGAATCATCCTGCGCAAGTGTTAGTGCTGGGTCCGGTTCCAATGTTTAACTGGATGCCTTTACAGCTTTCGGCGGGTATTACCTTATTTAGCTTATTCTTATTAAGTCTAGGTGTTTACGGTTTGATTTTGCCGTTAGAGCGCAAAATCCGACAGGTGCGTTATGCATTAAACCGTATGAAATCGGGTGATTTGTCACTGCGTGTTCCTATTGAGGGAAGCGATGAAATGGCAAACTTGGCTTCAAGTTATAACAATATGTCTGACCATATTCAGCGTTTGATTGAGGCTCAACGTGAGTTAATGAGAGCAGTATCTCATGAACTCAGAACACCTGTGGCACGTATTCGCTTTGGTACAGAAATGTTAGCCGAAGAAGATGATTACAATCATCGTATGCATCAGGTCGACATGATTGATAAAGATATTGAAGCGCTCAATACCTTAATTGATGAAATCATGACTTATGCAAAACTAGAGCAGGGTACACCTTCATTAGACTTTGCAGAAATTGTACTTTTTGAAGTGTTGGATCAGGTTGCAGTTGAAACTGAGGCTTTAAAAACACAGAAAGAAATTGAACTTATTCCACCGCCTTTATATGTGAAAGTAGATGCAGAGCGTCGTTACCTTCACCGTGTCGTGCAAAATCTAGTAGGTAATGCAGTTCGTTATTGTGATAACAAAGTTCGGATTACGGGCGGTATTCATAATGATGGCATGGCTTTTGTCTGTGTCGAAGACGACGGACCGGGTATTCCTGAGCAAGACCGCAAGCGTGTATTTGAAGCCTTTGCCCGTTTAGATGATAGCCGTACACGTGCATCTGGCGGTTATGGTTTGGGTCTCTCTATCGTAAGTCGTATTGCTTACTGGTTTGGTGGGGAAATTAAGGTAGATGAAAGTCCAAGTTTGGGAGGCGCACGCTTTATTATGACTTGGCCAGCACATCGCTTTAAACAACCGCCATTAAAGACCAATAAAAAAGCACCTGCA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 1,717 total letters Query= IDP95892.107 Length=1965 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2814 0.0 Q I I N Y Q R A Q E Y R E S L T D G I S Y V I S E G V A R Q Query_1 202 CAAATTATCAACTACCAACGCGCACAAGAATACCGAGAATCTTTAACTGATGGTATTTCTTATGTCATTAGTGAAGGAGTCGCTCGACAA 291 0 1 .......................................................................................... 90 P G K Q Q K I D W I S D A S D L L E L P I Y Y T D A S K V E Query_1 292 CCTGGGAAGCAGCAAAAAATAGATTGGATTTCTGACGCATCTGATTTGCTCGAACTTCCAATTTATTATACCGATGCAAGTAAGGTTGAG 381 0 91 .......................................................................................... 180 L S R T E K K R I E A Q K S V V R Y D A S N S I A Y V I I G Query_1 382 CTGTCTCGGACCGAGAAAAAGCGGATAGAAGCACAAAAATCTGTAGTTCGTTACGATGCAAGCAATAGCATTGCATATGTCATTATTGGC 471 0 181 .......................................................................................... 270 0 356 ........... 346 L R D D P Q H Y L S I K V D K I T E R Q M K A L P I F V L D Query_1 472 TTACGTGACGACCCACAACATTACTTATCTATAAAAGTCGACAAAATTACTGAACGCCAAATGAAGGCTCTTCCTATTTTTGTGCTCGAC 561 0 271 .......................................................................................... 360 0 220 ........... 210 Y L M F Y P G Q E Q E Y L A K I Q K H F S Y P I N I Q N I Q Query_1 562 TATTTAATGTTTTATCCGGGGCAGGAACAAGAATATCTTGCCAAGATTCAAAAGCATTTCTCATATCCGATCAATATTCAAAATATTCAA 651 0 361 .......................................................................................... 450 D V N L D S E Q I G R L R Q D Q S V M L Y K D S A T V R G T Query_1 652 GATGTAAATCTGGATTCTGAACAGATTGGACGTTTGCGCCAAGACCAAAGTGTCATGTTGTACAAAGATAGCGCAACAGTGCGCGGTACA 741 0 451 .......................................................................................... 540 T I S I V S P I P N H P A Q V L V L G P V P M F N W M P L Q Query_1 742 ACCATTTCGATTGTGTCTCCAATACCGAATCATCCTGCGCAAGTGTTAGTGCTGGGTCCGGTTCCAATGTTTAACTGGATGCCTTTACAG 831 0 541 .......................................................................................... 630 L S A G I T L F S L F L L S L G V Y G L I L P L E R K I R Q Query_1 832 CTTTCGGCGGGTATTACCTTATTTAGCTTATTCTTATTAAGTCTAGGTGTTTACGGTTTGATTTTGCCGTTAGAGCGCAAAATCCGACAG 921 0 631 .......................................................................................... 720 V R Y A L N R M K S G D L S L R V P I E G S D E M A N L A S Query_1 922 GTGCGTTATGCATTAAACCGTATGAAATCGGGTGATTTGTCACTGCGTGTTCCTATTGAGGGAAGCGATGAAATGGCAAACTTGGCTTCA 1011 0 721 .......................................................................................... 810 S Y N N M S D H I Q R L I E A Q R E L M R A V S H E L R T P Query_1 1012 AGTTATAACAATATGTCTGACCATATTCAGCGTTTGATTGAGGCTCAACGTGAGTTAATGAGAGCAGTATCTCATGAACTCAGAACACCT 1101 0 811 .......................................................................................... 900 V A R I R F G T E M L A E E D D Y N H R M H Q V D M I D K D Query_1 1102 GTGGCACGTATTCGCTTTGGTACAGAAATGTTAGCCGAAGAAGATGATTACAATCATCGTATGCATCAGGTCGACATGATTGATAAAGAT 1191 0 901 .......................................................................................... 990 I E A L N T L I D E I M T Y A K L E Q G T P S L D F A E I V Query_1 1192 ATTGAAGCGCTCAATACCTTAATTGATGAAATCATGACTTATGCAAAACTAGAGCAGGGTACACCTTCATTAGACTTTGCAGAAATTGTA 1281 0 991 .......................................................................................... 1080 L F E V L D Q V A V E T E A L K T Q K E I E L I P P P L Y V Query_1 1282 CTTTTTGAAGTGTTGGATCAGGTTGCAGTTGAAACTGAGGCTTTAAAAACACAGAAAGAAATTGAACTTATTCCACCGCCTTTATATGTG 1371 0 1081 .......................................................................................... 1170 K V D A E R R Y L H R V V Q N L V G N A V R Y C D N K V R I Query_1 1372 AAAGTAGATGCAGAGCGTCGTTACCTTCACCGTGTCGTGCAAAATCTAGTAGGTAATGCAGTTCGTTATTGTGATAACAAAGTTCGGATT 1461 0 1171 .......................................................................................... 1260 T G G I H N D G M A F V C V E D D G P G I P E Q D R K R V F Query_1 1462 ACGGGCGGTATTCATAATGATGGCATGGCTTTTGTCTGTGTCGAAGACGACGGACCGGGTATTCCTGAGCAAGACCGCAAGCGTGTATTT 1551 0 1261 .......................................................................................... 1350 E A F A R L D D S R T R A S G G Y G L G L S I V S R I A Y W Query_1 1552 GAAGCCTTTGCCCGTTTAGATGATAGCCGTACACGTGCATCTGGCGGTTATGGTTTGGGTCTCTCTATCGTAAGTCGTATTGCTTACTGG 1641 0 1351 .......................................................................................... 1440 F G G E I K V D E S P S L G G A R F I M T W P A H R F K Q P Query_1 1642 TTTGGTGGGGAAATTAAGGTAGATGAAAGTCCAAGTTTGGGAGGCGCACGCTTTATTATGACTTGGCCAGCACATCGCTTTAAACAACCG 1731 0 1441 .......................................................................................... 1530 P L K T N K K A P A Query_1 1732 CCATTAAAGACCAATAAAAAAGCACCTGCA 1761 0 1531 .............................. 1560 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3252881 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 1,717 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95893.103 (IDP95893.1-290.F..pMCSG73)
MKKFLGAYQNNHMHWVGDGFPVYNLFSYDRLGQTLSPFLLLDYAAPYNFSPTTEQQGVGSHPHRGFETVTIAYQGEVTHKDSSGGGGTIKTGDVQWMTAGAGVLHEEFHSPEFAEHGGLFEMVQLWVNLPSHSKMTPGKYQAIEAKDIPDIALDEHGSHLRVIAGEYADAKGAATTFSPLNVWDGKLVKGQKHTLYVPEGHTTLVVVLEGAVVVNDTNRLEGKTVAILSREGVEFSLNAEEDTKFLVLTGQPLNEPIEGYGPFVMNTKAEIMEAINDFNRGKFGSIMQEG
ATGAAGAAATTTCTTGGTGCATATCAAAATAACCACATGCATTGGGTTGGTGATGGTTTCCCTGTATATAACCTGTTCTCTTATGACCGTTTAGGCCAAACCCTAAGCCCATTTTTGTTACTGGATTATGCAGCGCCATATAACTTCTCACCAACCACCGAACAACAAGGTGTGGGTTCACATCCTCACCGTGGCTTCGAAACAGTTACTATTGCCTATCAAGGTGAAGTGACTCATAAAGACTCAAGTGGTGGTGGCGGAACCATTAAAACCGGTGACGTGCAATGGATGACTGCGGGTGCAGGGGTGTTACATGAAGAGTTCCATTCTCCTGAATTTGCAGAGCATGGCGGTCTTTTTGAAATGGTGCAATTGTGGGTAAATTTACCTTCGCATTCAAAAATGACCCCTGGAAAATATCAAGCCATTGAAGCTAAAGATATTCCGGACATTGCACTAGACGAGCACGGTAGTCATTTGCGTGTGATCGCAGGTGAATATGCAGATGCAAAAGGTGCAGCAACTACATTTAGTCCATTAAATGTATGGGACGGTAAATTGGTAAAAGGCCAAAAACATACCCTGTATGTACCTGAAGGGCATACTACCCTTGTTGTTGTGCTAGAGGGTGCGGTTGTGGTCAATGATACAAACCGTCTTGAAGGCAAAACCGTTGCGATTTTATCTCGTGAGGGCGTTGAGTTTAGCTTAAATGCTGAAGAAGACACTAAATTCTTAGTGTTGACTGGCCAACCACTAAATGAGCCAATCGAAGGTTATGGTCCATTTGTCATGAATACCAAAGCCGAGATTATGGAAGCGATTAATGATTTTAATCGTGGAAAATTCGGTTCAATCATGCAAGAAGGA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 1,900 total letters Query= IDP95893.103 Length=1275 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1570 0.0 2_12D_D6_IDP95893.103_T7term.seq 1445 0.0 N L Y F Q S N A M K K F L G A Y Q N N H M H W V G D G F P Query_1 176 AGAACCTGTACTTCCAATCCAATGCCATGAAGAAATTTCTTGGTGCAT-ATCAAAATAACCACATGCATTGGGTTGGTGATGGTTTCCCT 264 0 1 ......................-......................................... 63 0 15 ............ 4 2 963 .....-....N.N.-.......NC.N.-......-...N..N......N......-..N.............-....N........T..N 880 V Y N L F S Y D R L G Q T L S P F L L L D Y A A P Y N F S Query_1 265 GTATATAACCTGTT-CTCTTATGACCGTTTAGGCCAAACCCTAAG-CCCATTTTTGTTACTGGATTATGCAGCGCCATATAACTTCTCAC 352 0 64 ..............-..............................-............................................ 151 2 879 .G.....-......T....N............NG......N...NN.................N...N.............N........ 791 P T T E Q Q G V G S H P H R G F E T V T I A Y Q G E V T H Query_1 353 CAACCACCGAACAACAAGG-TGTGGGTTCACATCCTCACCGTGGCTT-CGAAACAGTTACTATTGCCTATCAA-GGTGAAGTGACTCATA 439 0 152 ...................-...........................-.........................-................ 238 2 790 ..........N.N...T..G...................N.......TN........................A.......A........ 701 K D S S G G G G T I K T G D V Q W M T A G A G V L H E E F H Query_1 440 AAGACTCAAGTGGTGGTGGCGGAACCATTAAAACCGGTGACGTGCAATGGATGACTGCGGGTGCAGGGG-TGTTACATGAAGAGTTCCAT 528 0 239 .....................................................................-.................... 327 2 700 .....................................................................G.................... 611 S P E F A E H G G L F E M V Q L W V N L P S H S K M T P G K Query_1 529 TCTCCTGAATTTGCAGAGCATGGCGGTCTTTTTGAAATGGTGCAATTGTGGGTAAATTTACCTTCGCATTCAAAAATGACCCCTGGAAAA 618 0 328 .......................................................................................... 417 0 389 ............ 378 2 610 ...........................N....................................N......................... 521 2 549 ............ 560 Y Q A I E A K D I P D I A L D E H G S H L R V I A G E Y A D Query_1 619 TATCAAGCCATTGAAGCTAAAGATATTCCGGACATTGCACTAGACGAGCACGGTAGTCATTTGCGTGTGATCGCAGGTGAATATGCAGAT 708 0 418 .......................................................................................... 507 2 520 .......................................................................................... 431 A K G A A T T F S P L N V W D G K L V K G Q K H T L Y V P E Query_1 709 GCAAAAGGTGCAGCAACTACATTTAGTCCATTAAATGTATGGGACGGTAAATTGGTAAAAGGCCAAAAACATACCCTGTATGTACCTGAA 798 0 508 .......................................................................................... 597 2 430 .......................................................................................... 341 G H T T L V V V L E G A V V V N D T N R L E G K T V A I L S Query_1 799 GGGCATACTACCCTTGTTGTTGTGCTAGAGGGTGCGGTTGTGGTCAATGATACAAACCGTCTTGAAGGCAAAACCGTTGCGATTTTATCT 888 0 598 .......................................................................................... 687 2 340 .......................................................................................... 251 R E G V E F S L N A E E D T K F L V L T G Q P L N E P I E G Query_1 889 CGTGAGGGCGTTGAGTTTAGCTTAAATGCTGAAGAAGACACTAAATTCTTAGTGTTGACTGGCCAACCACTAAATGAGCCAATCGAAGGT 978 0 688 .......................................................................................... 777 2 250 .......................................................................................... 161 Y G P F V M N T K A E I M E A I N D F N R G K F G S I M Q E Query_1 979 TATGGTCCATTTGTCATGAATACCAAAGCCGAGATTATGGAAGCGATTAATGATTTTAATCGTGGAAAATTCGGTTCAATCATGCAAGAA 1068 0 778 .......................................................................................... 867 2 160 .......................................................................................... 71 G * H W K W I T D P N S S S V D K L A A A L E H H H H H H * Query_1 1069 GGATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGA 1158 0 868 ... 870 2 70 .......................--..... 43 D P A A N K A R K E Query_1 1159 GATCCGGCTGCT-AACAAAGCCC---GAAAGGAAG 1189 2 47 .......N...A..........CGAA........ 14 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2337300 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 1,900 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95895.103 (IDP95895.25-394.P(394)..pMCSG68)
APVVKEKLSPLHPDSPESEESYAEVPIESIQQFVQIYGIVRDNYVDEKSDDALFLQAIKGLVSGLDRYSRYLSAEEYRQLIQYTEGDLASVDFVLSPESHVHKWMIRDLKTGSDSYKLGLRNGQTILKIDNQELKNLTHDQVLGLLYGSIGSTLQVQTEESNSPISLVRNKKIETDIEPVMLHNQVLVLKIRVFQQDTANEIKRLIEENSSSRLKAVLIDLRNNPGGLLSAAVESADLFLNHGIIVSTKSRSEGNQQFQALPGNDFQNIKVGILINHRSASAAEVFTAAMKEHQRAWVMGEKSYGKGVVQKLFPLPSGAALQMTVSHYYTPNGNMIEGQGIQPNQTYPLPPEMKEEVYLDRVADLLLKRK
GCTCCTGTTGTAAAAGAAAAATTATCGCCTCTGCATCCTGATTCCCCGGAATCAGAAGAAAGTTATGCAGAAGTGCCTATTGAGTCTATTCAACAGTTTGTACAAATATATGGGATTGTCCGTGATAATTATGTCGATGAAAAATCGGACGATGCTTTATTTTTACAGGCAATTAAAGGTTTAGTCAGTGGTCTAGACCGTTACTCCCGCTATTTGTCAGCTGAAGAATACCGTCAACTCATTCAATATACTGAAGGGGATCTTGCTTCAGTTGATTTTGTACTAAGCCCCGAATCACATGTTCATAAATGGATGATTCGTGACCTTAAGACGGGTTCTGACTCATATAAACTAGGCTTAAGAAATGGCCAAACTATTTTAAAGATTGATAATCAGGAACTTAAAAATCTGACACATGATCAGGTGCTAGGTTTGCTTTATGGATCGATAGGCTCGACGCTTCAAGTACAAACAGAAGAGTCAAATAGTCCAATTAGTTTAGTCCGTAATAAAAAGATTGAAACCGATATTGAGCCTGTGATGTTGCACAATCAGGTGTTGGTATTAAAAATTAGAGTATTTCAACAAGATACCGCCAATGAGATTAAGCGTTTAATTGAAGAAAATAGTTCTTCGCGTTTAAAAGCGGTGTTAATTGATTTAAGAAATAACCCAGGCGGCTTGTTATCTGCTGCTGTTGAGTCAGCCGATTTATTTTTAAATCATGGCATTATTGTCTCTACAAAAAGTCGTTCAGAAGGCAATCAGCAATTTCAGGCTTTGCCAGGTAACGATTTTCAAAATATAAAAGTTGGTATTTTAATTAATCACCGATCTGCCTCGGCAGCAGAAGTTTTTACTGCTGCAATGAAAGAGCATCAACGTGCTTGGGTCATGGGTGAAAAAAGTTATGGAAAGGGTGTTGTGCAAAAGCTTTTCCCTCTACCAAGTGGGGCAGCGCTACAAATGACAGTCTCACATTACTACACACCAAATGGTAATATGATTGAGGGGCAAGGTATTCAGCCTAACCAGACTTATCCCTTACCCCCAGAAATGAAAGAAGAAGTTTATCTAGATCGTGTTGCCGATCTCCTTCTAAAAAGAAAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,334 total letters Query= IDP95895.103 Length=1515 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2003 0.0 1_01D_D1_IDP95895.103_T7sh.seq 1451 0.0 2_07D_D1_IDP95895.103_T7term.seq 491 3e-142 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 60 CCCCTCTAGAAATAATTTTG--TTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGG 147 1 20 ...NN.N............TGT.................................................................... 109 S H P Q F E K G T E N L Y F Q S N A A P V V K E K L S P L H Query_1 148 TCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGCTCCTGTTGTAAAAGAAAAATTATCGCCTCTGCAT 237 0 1 .................................... 36 1 110 .......................................................................................... 199 P D S P E S E E S Y A E V P I E S I Q Q F V Q I Y G I V R D Query_1 238 CCTGATTCCCCGGAATCAGAAGAAAGTTATGCAGAAGTGCCTATTGAGTCTATTCAACAGTTTGTACAAATATATGGGATTGTCCGTGAT 327 0 37 .......................................................................................... 126 1 200 .......................................................................................... 289 N Y V D E K S D D A L F L Q A I K G L V S G L D R Y S R Y L Query_1 328 AATTATGTCGATGAAAAATCGGACGATGCTTTATTTTTACAGGCAATTAAAGGTTTAGTCAGTGGTCTAGACCGTTACTCCCGCTATTTG 417 0 127 .......................................................................................... 216 1 290 .......................................................................................... 379 S A E E Y R Q L I Q Y T E G D L A S V D F V L S P E S H V H Query_1 418 TCAGCTGAAGAATACCGTCAACTCATTCAATATACTGAAGGGGATCTTGCTTCAGTTGATTTTGTACTAAGCCCCGAATCACATGTTCAT 507 0 217 .......................................................................................... 306 1 380 .......................................................................................... 469 K W M I R D L K T G S D S Y K L G L R N G Q T I L K I D N Q Query_1 508 AAATGGATGATTCGTGACCTTAAGACGGGTTCTGACTCATATAAACTAGGCTTAAGAAATGGCCAAACTATTTTAAAGATTGATAATCAG 597 0 307 .......................................................................................... 396 1 470 .......................................................................................... 559 E L K N L T H D Q V L G L L Y G S I G S T L Q V Q T E E S Query_1 598 GAACTTAAAAATCTGACACATGATCAGGTGCTAGGTTTGC-TTTATGGATCGATAGGCTCGACGCTTCAAGTACAAACAGAAGAGTCAAA 686 0 397 ........................................-................................................. 485 1 560 ........................................T................................................. 649 N S P I S L V R N K K I E T D I E P V M L H N Q V L V L K I Query_1 687 TAGTCCAATTAGTTTAGTCCGTAATAAAAAGATTGAAACCGATATTGAGCCTGTGATGTTGCACAATCAGGTGTTGGTATTAAAAATTAG 776 0 486 .......................................................................................... 575 1 650 ...................T.............................N.............N.N.......N................ 739 R V F Q Q D T A N E I K R L I E E N S S S R L K A V L I D L Query_1 777 AGTATTTCAACAAGATACCGCCAATGAGATTAAGCGTTTAATTGAAGAAAATAGTT-CTTCGCGTTTAAAAGCGGTGTTAATTGATTTAA 865 0 576 ........................................................-................................. 664 1 740 ............N....................................N......C........N...................N..-. 828 R N N P G G L L S A A V E S A D L F L N H G I I V S T K S R Query_1 866 GAAATAACCCAGGCGGCTTGTTATCTGCTGCTGTTGAGTCAGCCGATTTATTTTTAAATCATGGCATTATTGTCTCTACAAAAAGTCGTT 955 0 665 .......................................................................................... 754 1 829 ...N.-..............-..A...N.........N...T......--N...C..NN....N......G.C...-........... 911 S E G N Q Q F Q A L P G N D F Q N I K V G I L I N H R S A S Query_1 956 CAGAAGGCAATCAGCAATTTCAGGCTTTGCCAGGTAACGATTTTCAAAATATAAAAGTTGGTATTTTAATTAATCACCGATCTGCCTCGG 1045 0 755 .......................................................................................... 844 A A E V F T A A M K E H Q R A W V M G E K S Y G K G V V Q K Query_1 1046 CAGCAGAAGTTTTTACTGCTGCAATGAAAGAGCATCAACGTGCTTGGGTCATGGGTGAAAAAAGTTATGGAAAGGGTGTTGTGCAAAAGC 1135 0 845 .......................................................................................... 934 2 313 .....N...................... 286 L F P L P S G A A L Q M T V S H Y Y T P N G N M I E G Q G I Query_1 1136 TTTTCCCTCTACCAAGTGGGGCAGCGCTACAAATGACAGTCTCACATTACTACACACCAAATGGTAATATGATTGAGGGGCAAGGTATTC 1225 0 935 .......................................................................................... 1024 2 285 ................N...............................NN...........N............................ 196 Q P N Q T Y P L P P E M K E E V Y L D R V A D L L L K R K * Query_1 1226 AGCCTAACCAGACTTATCCCTTACCCCCAGAAATGAAAGAAGAAGTTTATCTAGATCGTGTTGCCGATCTCCTTCTAAAAAGAAAGTAAC 1315 0 1025 ...................................................................................... 1110 2 195 ..................................................N.......................N............... 106 H W K W I T D P N S S S V D K L A A A L E H H H H H H * D Query_1 1316 ATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATC 1402 2 105 .A................................N.................N..........................N....... 19 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3426714 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 2,334 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95895.105 (IDP95895.25-394.P(394)..pMCSG73)
APVVKEKLSPLHPDSPESEESYAEVPIESIQQFVQIYGIVRDNYVDEKSDDALFLQAIKGLVSGLDRYSRYLSAEEYRQLIQYTEGDLASVDFVLSPESHVHKWMIRDLKTGSDSYKLGLRNGQTILKIDNQELKNLTHDQVLGLLYGSIGSTLQVQTEESNSPISLVRNKKIETDIEPVMLHNQVLVLKIRVFQQDTANEIKRLIEENSSSRLKAVLIDLRNNPGGLLSAAVESADLFLNHGIIVSTKSRSEGNQQFQALPGNDFQNIKVGILINHRSASAAEVFTAAMKEHQRAWVMGEKSYGKGVVQKLFPLPSGAALQMTVSHYYTPNGNMIEGQGIQPNQTYPLPPEMKEEVYLDRVADLLLKRK
GCTCCTGTTGTAAAAGAAAAATTATCGCCTCTGCATCCTGATTCCCCGGAATCAGAAGAAAGTTATGCAGAAGTGCCTATTGAGTCTATTCAACAGTTTGTACAAATATATGGGATTGTCCGTGATAATTATGTCGATGAAAAATCGGACGATGCTTTATTTTTACAGGCAATTAAAGGTTTAGTCAGTGGTCTAGACCGTTACTCCCGCTATTTGTCAGCTGAAGAATACCGTCAACTCATTCAATATACTGAAGGGGATCTTGCTTCAGTTGATTTTGTACTAAGCCCCGAATCACATGTTCATAAATGGATGATTCGTGACCTTAAGACGGGTTCTGACTCATATAAACTAGGCTTAAGAAATGGCCAAACTATTTTAAAGATTGATAATCAGGAACTTAAAAATCTGACACATGATCAGGTGCTAGGTTTGCTTTATGGATCGATAGGCTCGACGCTTCAAGTACAAACAGAAGAGTCAAATAGTCCAATTAGTTTAGTCCGTAATAAAAAGATTGAAACCGATATTGAGCCTGTGATGTTGCACAATCAGGTGTTGGTATTAAAAATTAGAGTATTTCAACAAGATACCGCCAATGAGATTAAGCGTTTAATTGAAGAAAATAGTTCTTCGCGTTTAAAAGCGGTGTTAATTGATTTAAGAAATAACCCAGGCGGCTTGTTATCTGCTGCTGTTGAGTCAGCCGATTTATTTTTAAATCATGGCATTATTGTCTCTACAAAAAGTCGTTCAGAAGGCAATCAGCAATTTCAGGCTTTGCCAGGTAACGATTTTCAAAATATAAAAGTTGGTATTTTAATTAATCACCGATCTGCCTCGGCAGCAGAAGTTTTTACTGCTGCAATGAAAGAGCATCAACGTGCTTGGGTCATGGGTGAAAAAAGTTATGGAAAGGGTGTTGTGCAAAAGCTTTTCCCTCTACCAAGTGGGGCAGCGCTACAAATGACAGTCTCACATTACTACACACCAAATGGTAATATGATTGAGGGGCAAGGTATTCAGCCTAACCAGACTTATCCCTTACCCCCAGAAATGAAAGAAGAAGTTTATCTAGATCGTGTTGCCGATCTCCTTCTAAAAAGAAAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 1,343 total letters Query= IDP95895.105 Length=1515 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2003 0.0 A P V V K E K L S P L H P D S P E S E E S Y A E V P I E S I Query_1 202 GCTCCTGTTGTAAAAGAAAAATTATCGCCTCTGCATCCTGATTCCCCGGAATCAGAAGAAAGTTATGCAGAAGTGCCTATTGAGTCTATT 291 0 1 .......................................................................................... 90 Q Q F V Q I Y G I V R D N Y V D E K S D D A L F L Q A I K G Query_1 292 CAACAGTTTGTACAAATATATGGGATTGTCCGTGATAATTATGTCGATGAAAAATCGGACGATGCTTTATTTTTACAGGCAATTAAAGGT 381 0 91 .......................................................................................... 180 L V S G L D R Y S R Y L S A E E Y R Q L I Q Y T E G D L A S Query_1 382 TTAGTCAGTGGTCTAGACCGTTACTCCCGCTATTTGTCAGCTGAAGAATACCGTCAACTCATTCAATATACTGAAGGGGATCTTGCTTCA 471 0 181 .......................................................................................... 270 V D F V L S P E S H V H K W M I R D L K T G S D S Y K L G L Query_1 472 GTTGATTTTGTACTAAGCCCCGAATCACATGTTCATAAATGGATGATTCGTGACCTTAAGACGGGTTCTGACTCATATAAACTAGGCTTA 561 0 271 .......................................................................................... 360 R N G Q T I L K I D N Q E L K N L T H D Q V L G L L Y G S I Query_1 562 AGAAATGGCCAAACTATTTTAAAGATTGATAATCAGGAACTTAAAAATCTGACACATGATCAGGTGCTAGGTTTGCTTTATGGATCGATA 651 0 361 .......................................................................................... 450 G S T L Q V Q T E E S N S P I S L V R N K K I E T D I E P V Query_1 652 GGCTCGACGCTTCAAGTACAAACAGAAGAGTCAAATAGTCCAATTAGTTTAGTCCGTAATAAAAAGATTGAAACCGATATTGAGCCTGTG 741 0 451 .......................................................................................... 540 M L H N Q V L V L K I R V F Q Q D T A N E I K R L I E E N S Query_1 742 ATGTTGCACAATCAGGTGTTGGTATTAAAAATTAGAGTATTTCAACAAGATACCGCCAATGAGATTAAGCGTTTAATTGAAGAAAATAGT 831 0 541 .......................................................................................... 630 S S R L K A V L I D L R N N P G G L L S A A V E S A D L F L Query_1 832 TCTTCGCGTTTAAAAGCGGTGTTAATTGATTTAAGAAATAACCCAGGCGGCTTGTTATCTGCTGCTGTTGAGTCAGCCGATTTATTTTTA 921 0 631 .......................................................................................... 720 N H G I I V S T K S R S E G N Q Q F Q A L P G N D F Q N I K Query_1 922 AATCATGGCATTATTGTCTCTACAAAAAGTCGTTCAGAAGGCAATCAGCAATTTCAGGCTTTGCCAGGTAACGATTTTCAAAATATAAAA 1011 0 721 .......................................................................................... 810 V G I L I N H R S A S A A E V F T A A M K E H Q R A W V M G Query_1 1012 GTTGGTATTTTAATTAATCACCGATCTGCCTCGGCAGCAGAAGTTTTTACTGCTGCAATGAAAGAGCATCAACGTGCTTGGGTCATGGGT 1101 0 811 .......................................................................................... 900 E K S Y G K G V V Q K L F P L P S G A A L Q M T V S H Y Y T Query_1 1102 GAAAAAAGTTATGGAAAGGGTGTTGTGCAAAAGCTTTTCCCTCTACCAAGTGGGGCAGCGCTACAAATGACAGTCTCACATTACTACACA 1191 0 901 .......................................................................................... 990 P N G N M I E G Q G I Q P N Q T Y P L P P E M K E E V Y L D Query_1 1192 CCAAATGGTAATATGATTGAGGGGCAAGGTATTCAGCCTAACCAGACTTATCCCTTACCCCCAGAAATGAAAGAAGAAGTTTATCTAGAT 1281 0 991 .......................................................................................... 1080 R V A D L L L K R K Query_1 1282 CGTGTTGCCGATCTCCTTCTAAAAAGAAAG 1311 0 1081 .............................. 1110 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1947000 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 1,343 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96096.102 (IDP96096.1-1411.F..pMCSG68)
MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVTSGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADIRLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGRTQTFHREAAGEFSGEITGVTDGAWRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAVVYDRSGKQVRSFTYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLDRREVLHTQGEAGLKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHNQLTSATGPDGLELRREYDELGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQLIAVKDTQGHETRYEYNIAGDLTAVIAPDGSRNGTQYDAWGKAVRTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRLTHRTVKGETAERWQYDERGWLTDISHISEGHRVAVHYRYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGYLAGMKLGDTPLVEYTRDRLHRETLRSFGRYELTTAYTPAGQLQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLTGVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDRHGRLTEKTDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYEEPLVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRSRIQTIYQPGSFTPLIRVETATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLPLALISTEGATAWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYGYQLNPISDIDPLGLSMWEDAKSGACTNGLCGTLSAMIGPDKFDSIDSTAYDALNKINSQSICEDKEFAGLICKDNSGRYFSTAPNRGERKGSYPFNSPCPNGTEKVSAYHTHGADSHGEYWDEIFSGKDEKIVKSKDNNIKSFYLGTPSGNFKAIDNHGKEITNRKGLPNVCRVHGNM
ATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATAACACACTGATACTCAGTGATAACGGCGGCAGAAGCCTGTATTTTGAGCACCTGTTTCCCGGTGAGGACGGTTACAGCCGCAGCGAGTCACTGTGGCTGGTGCGCGGCGGCGTGGCGAAACTGGATGAAGGTCACCGGCTGGCCGCACTCTGGCAGGCGCTGCCGGAAGAACTCCGCTTAAGTCCGCATCGTTATCTGGCGACAAACAGTCCGCAGGGGCCGTGGTGGCTGCTCGGTTGGTGTGAGCGGGTGCCGGAAGCGGATGAGGTGCTGCCTGCGCCGCTGCCGCCGTACCGGGTACTGACCGGGCTGGTGGACCGCTTCGGGCGCACACAGACGTTCCACCGCGAAGCCGCCGGTGAATTCAGCGGCGAAATCACCGGCGTGACGGATGGTGCCTGGCGTCACTTCCGGCTGGTACTGACCACGCAGGCGCAGCGGGCAGAAGAAGCCCGGCAGCAGGCCATTTCCGGCGGGACGGAACCGTCCGCTTTTCCTGATACCCTGCCGGGTTACACCGAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTGGTGCGCTATGGCTGGACGCCACGCGGCGAACTGGCGGTGGTGTATGACCGTAGTGGCAAACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGGCCGGAAATCCGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACGCAGAGTCAGTTTGACGCCGTGGGCAGGCTCAGGGCACAGACGGATGCCGCAGGCAGGACAACAGAGTACAGCCCGGATGTGGTGACGGGCCTCATCACGCGCATAACCACGCCGGATGGCAGGGCATCGGCGTTTTACTATAACCACCACAACCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAATTGCGCCGGGAATATGATGAATTGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACAGTGACTTACCCTGCGCAACGGAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACCGACTGTTCCGGTTATGTAACCCGTTATGACCATGACCGCTTCGGGCAGATGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTACCGCGCATACGACAGCCGTGGACAGTTAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTGACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCACCTTCCGTTACGATGTACTTGACCGGCTGATACAGGAAACCGGCTTTGACGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTATCCGCAGCGAGGATGAGGGTCTGGTCACCCACTGGCACTATGACGAAGCAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCATATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCAGACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGCACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTTATACCCCTGCCGGGCAGTTACAGAGCCAGCACCTGAACAGCCTGCTGTCTGACCGCGATTACACCTGGAACGACAACGGCGAACTCATCCGCATCAGCAGCCCGCGCCAGACCCGGAGTTACAGCTACAGCACCACCGGCAGGCTGACCGGCGTTCACACCACCGCAGCGAATCTGGATATCCGCATCCCGTATGCCACAGACCCGGCAGGTAACCGCCTGCCCGACCCGGAGCTGCACCCGGACAGCACCCTCAGCATGTGGCCGGATAACCGTATCGCCCGTGACGCGCACTATCTTTACCGGTATGACCGTCACGGCAGGCTGACAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGGACTCACCGGTACCATTACGACAGTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGAAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGGTGGCAAAACGGGTGTGGCGGCGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACGGCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAACTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATGGATATCAATTGAATCCGATATCAGACATCGACCCCCTGGGTTTATCTATGTGGGAGGATGCAAAATCGGGGGCATGTACTAATGGTCTTTGCGGCACACTATCCGCTATGATAGGTCCAGATAAATTTGATTCTATAGATAGCACCGCATATGACGCCTTAAATAAAATAAATAGCCAATCTATTTGCGAAGATAAAGAGTTCGCTGGTTTAATATGTAAGGATAATAGTGGCAGATATTTCTCAACAGCACCTAACCGAGGAGAAAGAAAAGGATCATATCCATTCAATAGCCCTTGCCCTAATGGTACTGAGAAAGTATCAGCTTATCATACTCATGGTGCAGATAGTCATGGAGAATATTGGGACGAAATATTTTCAGGTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATAACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,747 total letters Query= IDP96096.102 Length=4638 Score E Sequences producing significant alignments: (Bits) Value 0_wt 7634 0.0 1_01E_E1_IDP96096.102_T7sh.seq 471 2e-135 2_07E_E1_IDP96096.102_T7term.seq 260 6e-72 W S H P Q F E K G T E N L Y F Q S N A M S G K P A A R Q G D Query_1 145 TGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGAGCGGAAAACCGGCGGCGCGTCAGGGTGAC 234 0 1 ................................. 33 1 26 ..T..............NT..........................................T............T........T...... 115 M T Q Y G G S I V Q G S A G V R I G A P T G V A C S V C P G Query_1 235 ATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGC 324 0 34 .......................................................................................... 123 0 270 ...........C.. 257 1 116 ........T.-...................................N...................N..N.................... 204 G V T S G H P V N P L L G A K V L P G E T D I A L P G P L P Query_1 325 GGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCG 414 0 124 .......................................................................................... 213 1 205 ........................................................................N................. 294 F I L S R T Y S S Y R T K T P A P V G S L G P G W K M P A D Query_1 415 TTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGAT 504 0 214 .......................................................................................... 303 0 3275 ....G.T..T....CA..TG....A..--.........G.. 3313 0 103 ..A........... 90 0 309 ...... 304 0 2695 ....... 2689 1 295 ..................... 315 I R L Q L R D N T L I L S D N G G R S L Y F E H L F P G E D Query_1 505 ATCCGCTTACAGCTGCGCGATAACACACTGATACTCAGTGATAACGGCGGCAGAAGCCTGTATTTTGAGCACCTGTTTCCCGGTGAGGAC 594 0 304 .......................................................................................... 393 0 2471 ...........CA.G. 2456 0 303 ...... 298 0 2688 .... 2685 G Y S R S E S L W L V R G G V A K L D E G H R L A A L W Q A Query_1 595 GGTTACAGCCGCAGCGAGTCACTGTGGCTGGTGCGCGGCGGCGTGGCGAAACTGGATGAAGGTCACCGGCTGGCCGCACTCTGGCAGGCG 684 0 394 .......................................................................................... 483 0 2455 ... 2453 L P E E L R L S P H R Y L A T N S P Q G P W W L L G W C E R Query_1 685 CTGCCGGAAGAACTCCGCTTAAGTCCGCATCGTTATCTGGCGACAAACAGTCCGCAGGGGCCGTGGTGGCTGCTCGGTTGGTGTGAGCGG 774 0 484 .......................................................................................... 573 V P E A D E V L P A P L P P Y R V L T G L V D R F G R T Q T Query_1 775 GTGCCGGAAGCGGATGAGGTGCTGCCTGCGCCGCTGCCGCCGTACCGGGTACTGACCGGGCTGGTGGACCGCTTCGGGCGCACACAGACG 864 0 574 .......................................................................................... 663 0 1663 ............. 1675 F H R E A A G E F S G E I T G V T D G A W R H F R L V L T T Query_1 865 TTCCACCGCGAAGCCGCCGGTGAATTCAGCGGCGAAATCACCGGCGTGACGGATGGTGCCTGGCGTCACTTCCGGCTGGTACTGACCACG 954 0 664 .......................................................................................... 753 Q A Q R A E E A R Q Q A I S G G T E P S A F P D T L P G Y T Query_1 955 CAGGCGCAGCGGGCAGAAGAAGCCCGGCAGCAGGCCATTTCCGGCGGGACGGAACCGTCCGCTTTTCCTGATACCCTGCCGGGTTACACC 1044 0 754 .......................................................................................... 843 0 1124 ........... 1114 E Y G R D N G I R L S A V W L T H D P E Y P E N L P A A P L Query_1 1045 GAATATGGCCGGGACAACGGCATCCGTCTGTCTGCCGTGTGGCTGACGCACGACCCGGAATACCCGGAGAATTTACCTGCCGCGCCGCTG 1134 0 844 .......................................................................................... 933 0 1312 .............. 1299 V R Y G W T P R G E L A V V Y D R S G K Q V R S F T Y D D K Query_1 1135 GTGCGCTATGGCTGGACGCCACGCGGCGAACTGGCGGTGGTGTATGACCGTAGTGGCAAACAGGTGCGCAGCTTTACTTACGATGATAAA 1224 0 934 .......................................................................................... 1023 Y R G R M V A H R H T G R P E I R Y R Y D S D G R V T E Q L Query_1 1225 TACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGGCCGGAAATCCGTTACCGTTACGACAGCGACGGGCGGGTGACAGAACAGCTA 1314 0 1024 .......................................................................................... 1113 N P A G L S Y T Y Q Y E K D R I T I T D S L D R R E V L H T Query_1 1315 AACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACG 1404 0 1114 .......................................................................................... 1203 0 1757 ..... 1761 0 1878 .... 1881 Q G E A G L K R V V K K E H A D G S V T Q S Q F D A V G R L Query_1 1405 CAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACGCAGAGTCAGTTTGACGCCGTGGGCAGGCTC 1494 0 1204 .......................................................................................... 1293 0 1762 ...... 1767 0 1823 ........... 1833 0 1882 ....... 1888 R A Q T D A A G R T T E Y S P D V V T G L I T R I T T P D G Query_1 1495 AGGGCACAGACGGATGCCGCAGGCAGGACAACAGAGTACAGCCCGGATGTGGTGACGGGCCTCATCACGCGCATAACCACGCCGGATGGC 1584 0 1294 .......................................................................................... 1383 0 874 .............. 861 R A S A F Y Y N H H N Q L T S A T G P D G L E L R R E Y D E Query_1 1585 AGGGCATCGGCGTTTTACTATAACCACCACAACCAGTTAACGTCAGCCACCGGGCCTGACGGGCTGGAATTGCGCCGGGAATATGATGAA 1674 0 1384 .......................................................................................... 1473 0 3027 ..G..A.............. 3046 0 3505 ............ 3516 L G R L I Q E T A P D G D I T R Y R Y D N P H S D L P C A T Query_1 1675 TTGGGCCGTCTGATTCAGGAAACTGCCCCTGACGGCGATATCACCCGCTACCGTTATGATAATCCACACAGTGACTTACCCTGCGCAACG 1764 0 1474 .......................................................................................... 1563 0 3517 .G.... 3522 E D A T G S R K T M T W S R Y G Q L L S F T D C S G Y V T R Query_1 1765 GAAGATGCCACCGGCAGCCGGAAAACCATGACGTGGAGCCGTTACGGTCAGTTGCTGAGCTTCACCGACTGTTCCGGTTATGTAACCCGT 1854 0 1564 .......................................................................................... 1653 0 3581 ....-............T.. 3563 Y D H D R F G Q M T A V H R E E G L S Q Y R A Y D S R G Q L Query_1 1855 TATGACCATGACCGCTTCGGGCAGATGACGGCGGTGCACCGCGAGGAAGGGCTGAGTCAGTACCGCGCATACGACAGCCGTGGACAGTTA 1944 0 1654 .......................................................................................... 1743 0 640 ............. 652 0 1695 ............ 1684 0 1822 .....-........... 1807 I A V K D T Q G H E T R Y E Y N I A G D L T A V I A P D G Query_1 1945 ATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACATCGCCGGTGACCTGACCGCCGTCAT-TGCCCCGGACGGCAG 2033 0 1744 ..........................................................................-............... 1832 0 1688 ...........C..... 1672 0 463 ........... 453 0 1247 .......... 1256 S R N G T Q Y D A W G K A V R T T Q G G L T R S M E Y D A A Query_1 2034 CAGAAACGGGACACAGTACGATGCGTGGGGAAAGGCCGTCCGTACCACGCAGGGCGGGCTGACGCGCAGTATGGAATACGATGCTGCCGG 2123 0 1833 .......................................................................................... 1922 0 1257 . 1257 G R V I R L T S E N G S H T T F R Y D V L D R L I Q E T G F Query_1 2124 ACGGGTCATCCGCCTGACCAGTGAAAACGGCAGCCACACCACCTTCCGTTACGATGTACTTGACCGGCTGATACAGGAAACCGGCTTTGA 2213 0 1923 .......................................................................................... 2012 D G R T Q R Y H H D L T G K L I R S E D E G L V T H W H Y D Query_1 2214 CGGCCGCACACAGCGTTATCACCACGACCTGACCGGCAAACTTATCCGCAGCGAGGATGAGGGTCTGGTCACCCACTGGCACTATGACGA 2303 0 2013 .......................................................................................... 2102 E A D R L T H R T V K G E T A E R W Q Y D E R G W L T D I S Query_1 2304 AGCAGACCGCCTCACGCACCGCACCGTGAAGGGTGAAACCGCAGAGCGGTGGCAGTATGACGAACGTGGCTGGCTGACAGACATCAGCCA 2393 0 2103 .......................................................................................... 2192 H I S E G H R V A V H Y R Y D E K G R L T G E R Q T V H H P Query_1 2394 TATCAGCGAAGGGCACCGGGTGGCGGTGCATTACAGGTATGATGAGAAAGGCCGGCTGACCGGTGAGCGTCAGACGGTGCATCACCCGCA 2483 0 2193 .......................................................................................... 2282 Q T E A L L W Q H E T R H A Y N A Q G L A N R C I P D S L P Query_1 2484 GACGGAAGCACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGC 2573 0 2283 .......................................................................................... 2372 A V E W L T Y G S G Y L A G M K L G D T P L V E Y T R D R L Query_1 2574 CGTGGAATGGCTGACCTACGGCAG-CGGTTACCTGGCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCGACCGCCTGC 2662 0 2373 ........................-................................................................. 2461 0 396 ....T.CT. 388 0 2732 .....G.......... 2717 H R E T L R S F G R Y E L T T A Y T P A G Q L Q S Q H L N S Query_1 2663 ACCGGGAAACGCTGCGCAGCTTCGGCCGTTATGAACTCACCACCGCTTATACCCCTGCCGGGCAGTTACAGAGCCAGCACCTGAACAGCC 2752 0 2462 .......................................................................................... 2551 0 387 .......... 378 L L S D R D Y T W N D N G E L I R I S S P R Q T R S Y S Y S Query_1 2753 TGCTGTCTGACCGCGATTACACCTGGAACGACAACGGCGAACTCATCCGCATCAGCAGCCCGCGCCAGACCCGGAGTTACAGCTACAGCA 2842 0 2552 .......................................................................................... 2641 T T G R L T G V H T T A A N L D I R I P Y A T D P A G N R L Query_1 2843 CCACCGGCAGGCTGACCGGCGTTCACACCACCGCAGCGAATCTGGATATCCGCATCCCGTATGCCACAGACCCGGCAGGTAACCGCCTGC 2932 0 2642 .......................................................................................... 2731 0 2826 ............ 2837 0 307 ........... 297 0 2406 ..........-.... 2393 P D P E L H P D S T L S M W P D N R I A R D A H Y L Y R Y D Query_1 2933 CCGACCCGGAGCTGCACCCGGACAGCACCCTCAGCATGTGGCCGGATAACCGTATCGCCCGTGACGCGCACTATCTTTACCGGTATGACC 3022 0 2732 .......................................................................................... 2821 0 2392 . 2392 R H G R L T E K T D L I P E G V I R T D D E R T H R Y H Y D Query_1 3023 GTCACGGCAGGCTGACAGAGAAAACCGACCTCATCCCGGAAGGGGTTATCCGCACGGATGATGAGCGGACTCACCGGTACCATTACGACA 3112 0 2822 .......................................................................................... 2911 0 2646 ............ 2657 S Q H R L V H Y T R T Q Y E E P L V E S R Y L Y D P L G R R Query_1 3113 GTCAGCACCGGCTGGTGCACTACACGCGGACACAATATGAAGAGCCGCTGGTCGAAAGTCGCTATCTTTACGACCCGCTGGGCCGCAGGG 3202 0 2912 .......................................................................................... 3001 V A K R V W R R E R D L T G W M S L S R K P Q V T W Y G W D Query_1 3203 TGGCAAAACGGGTGTGGCGGCGTGAACGGGACCTGACGGGCTGGATGTCGCTGTCACGGAAACCGCAAGTGACCTGGTACGGCTGGGACG 3292 0 3002 .......................................................................................... 3091 0 1432 ..C..G.............. 1451 G D R L T T I Q N D R S R I Q T I Y Q P G S F T P L I R V E Query_1 3293 GCGACCGGCTGACCACGATACAGAACGACAGGAGCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGGGTCGAAA 3382 0 3092 .......................................................................................... 3181 T A T G E L A K T Q R R S L A D A L Q Q S G G E D G G S V V Query_1 3383 CTGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCCCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGT 3472 0 3182 .......................................................................................... 3271 F P P V L V Q M L D R L E S E I L A D R V S E E S R R W L Query_1 3473 TCCCGCCGGTGCTGGTGCAGATGCTCGACC--GGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGC 3560 0 3272 ..............................--.......................................................... 3359 0 254 ....C.C..C....GG..GC....G..CC.........A.. 294 A S C G L T V E Q M Q N Q M D P V Y T P A R K I H L Y H C D Query_1 3561 ATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCA 3650 0 3360 .......................................................................................... 3449 H R G L P L A L I S T E G A T A W C A E Y D E W G N L L N E Query_1 3651 TCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGA 3740 0 3450 .......................................................................................... 3539 0 1462 .............T.... 1479 E N P H Q L Q Q L I R L P G Q Q Y D E E S G L Y Y N R H R Y Query_1 3741 GAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGG-GGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATT 3829 0 3540 ......................................-................................................... 3628 0 1588 ..T............T.... 1569 Y D P L Q G R Y I T Q D P I G L K G G W N L Y G Y Q L N P I Query_1 3830 ATGACCCGCTGCAGGGGCGATATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAACCTGTATGGATATCAATTGAATCCGATAT 3919 0 3629 .......................................................................................... 3718 S D I D P L G L S M W E D A K S G A C T N G L C G T L S A M Query_1 3920 CAGACATCGACCCCCTGGGTTTATCTATGTGGGAGGATGCAAAATCGGGGGCATGTACTAATGGTCTTTGCGGCACACTATCCGCTATGA 4009 0 3719 .......................................................................................... 3808 I G P D K F D S I D S T A Y D A L N K I N S Q S I C E D K E Query_1 4010 TAGGTCCAGATAAATTTGATTCTATAGATAGCACCGCATATGACGCCTTAAATAAAATAAATAGCCAATCTATTTGCGAAGATAAAGAGT 4099 0 3809 .......................................................................................... 3898 F A G L I C K D N S G R Y F S T A P N R G E R K G S Y P F N Query_1 4100 TCGCTGGTTTAATATGTAAGGATAATAGTGGCAGATATTTCTCAACAGCACCTAACCGAGGAGAAAGAAAAGGATCATATCCATTCAATA 4189 0 3899 .......................................................................................... 3988 S P C P N G T E K V S A Y H T H G A D S H G E Y W D E I F S Query_1 4190 GCCCTTGCCCTAATGGTACTGAGAAAGTATCAGCTTATCATACTCATGGTGCAGATAGTCATGGAGAATATTGGGACGAAATATTTTCAG 4279 0 3989 .......................................................................................... 4078 G K D E K I V K S K D N N I K S F Y L G T P S G N F K A I D Query_1 4280 GTAAAGATGAGAAAATAGTTAAAAGTAAAGATAACAATATCAAGTCATTTTATTTAGGTACGCCCAGTGGTAATTTTAAAGCAATAGATA 4369 0 4079 .......................................................................................... 4168 2 199 ... 197 N H G K E I T N R K G L P N V C R V H G N M * H W K W I T D Query_1 4370 ACCACGGGAAGGAAATAACAAACAGAAAAGGATTACCTAATGTCTGCAGAGTTCATGGTAATATGTAACATTGGAAGTGGATAACGGATC 4459 0 4169 ................................................................. 4233 2 196 .N.G................................N......N........................................A..... 107 P N S S S V D K L A A A L E H H H H H H * D P A A N K A R Query_1 4460 CGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCC--GAAA 4547 2 106 ..........N..............N................................G.....A.A..GN.T...A....N.NNG.... 17 K E Query_1 4548 GGAA 4551 2 16 .... 13 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 21681660 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 4,747 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96111.104 (IDP96111.1-809.F..pMCSG73)
MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAHPGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEGVLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLDNDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLFLPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAETSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLHLIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRLLQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMGNSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDGRYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALLVDIDELVQRCPQLVQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVLNWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLDIYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATNPTIAQGRVPLVLELLSPAQRPLQITRDLSDFWKGAYREVQKEMKGRYPKHVWPDDPANTAPTRRTKKYS
GTGTCGTCGTTGCCCGTTGCTGCCGTCTTACCTGAATTACTTACCGCTCTCGATTGTGCGCCGCAGGTATTATTAAGTGCGCCGACCGGGGCCGGGAAATCAACCTGGCTGCCGCTGCAACTGCTGGCGCATCCCGGCATTAACGGGAAAATTATCCTGCTGGAGCCGCGTCGTCTGGCGGCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTACCGGATGCGTGCGCAAAACTGCGTCGGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAGCGGTGTTGGACTGGTGATCCTTGATGAATTTCATGAGCGCAGCTTGCAGGCGGATTTGGCGTTGGCGCTGTTACTCGATGTGCAACAAGGTCTGCGTGATGACCTTAAACTGCTGATTATGTCGGCTACCCTGGACAACGACCGCTTGCAGCAAATGCTGCCAGAAGCGCCTGTCGTCATCTCAGAAGGGCGCTCGTTTCCGGTTGAACGCCGTTATTTACCGCTGCCCGCGCATCAGCGTTTTGACGATGCCGTTGCGGTAGCCACCGCTGAAATGCTGCGTCAGGAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTAGCGGCAATGCTGGTTAGCGCGAAGAACGACGACGAAGCTGCTACCGCGGCAAAAATTGCCGCCATTCTCGAAGAGCCGCCACGGATGGGCAATAGTGACCTGGGCGTGGCGTTTTCGCGCAATCAACCAGCCTGGCAGCAACGTAGTCAGCAACTGTTAAAACGCTTAAACGTACGTGGCGGTGAGGCAGACAGTTCGCTTATCGCGCCGCTACTTGCCGGGGCGTTTGCCGATCGCATTGCTCGTCGCCGTGGGCAAGATGGACGCTATCAACTGGCAAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACCGTTATTATTGCAGGGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTAGTACAACGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGACGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAGCGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGCCAGCGGTTGATGATGAAAGTTTATTGGCAGCGCTGGAAACGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCACTACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCATTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,491 total letters Query= IDP96111.104 Length=2832 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4378 0.0 2_12E_E6_IDP96111.104_T7term.seq 1582 0.0 V S S L P V A A V L P E L L T A L D C A P Q V L L S A P T G Query_1 202 GTGTCGTCGTTGCCCGTTGCTGCCGTCTTACCTGAATTACTTACCGCTCTCGATTGTGCGCCGCAGGTATTATTAAGTGCGCCGACCGGG 291 0 1 .......................................................................................... 90 A G K S T W L P L Q L L A H P G I N G K I I L L E P R R L A Query_1 292 GCCGGGAAATCAACCTGGCTGCCGCTGCAACTGCTGGCGCATCCCGGCATTAACGGGAAAATTATCCTGCTGGAGCCGCGTCGTCTGGCG 381 0 91 .......................................................................................... 180 A R N V A Q R L A E L L N E K P G D T V G Y R M R A Q N C V Query_1 382 GCGCGTAACGTCGCGCAACGGCTGGCGGAGCTGCTTAACGAAAAGCCAGGCGATACCGTTGGCTACCGGATGCGTGCGCAAAACTGCGTC 471 0 181 .......................................................................................... 270 G P N T R L E V V T E G V L T R M I Q R D P E L S G V G L V Query_1 472 GGGCCGAATACCCGCCTGGAAGTGGTTACCGAAGGCGTGCTGACGCGCATGATCCAGCGTGACCCGGAACTGAGCGGTGTTGGACTGGTG 561 0 271 .......................................................................................... 360 I L D E F H E R S L Q A D L A L A L L L D V Q Q G L R D D L Query_1 562 ATCCTTGATGAATTTCATGAGCGCAGCTTGCAGGCGGATTTGGCGTTGGCGCTGTTACTCGATGTGCAACAAGGTCTGCGTGATGACCTT 651 0 361 .......................................................................................... 450 K L L I M S A T L D N D R L Q Q M L P E A P V V I S E G R S Query_1 652 AAACTGCTGATTATGTCGGCTACCCTGGACAACGACCGCTTGCAGCAAATGCTGCCAGAAGCGCCTGTCGTCATCTCAGAAGGGCGCTCG 741 0 451 .......................................................................................... 540 F P V E R R Y L P L P A H Q R F D D A V A V A T A E M L R Q Query_1 742 TTTCCGGTTGAACGCCGTTATTTACCGCTGCCCGCGCATCAGCGTTTTGACGATGCCGTTGCGGTAGCCACCGCTGAAATGCTGCGTCAG 831 0 541 .......................................................................................... 630 E S G S L L L F L P G V G E I Q R V Q E Q L A S R I G S D V Query_1 832 GAAAGCGGATCATTACTGTTATTTTTACCTGGCGTCGGAGAAATTCAGCGTGTGCAGGAACAACTGGCTTCGCGCATCGGCAGTGATGTA 921 0 631 .......................................................................................... 720 0 1751 ........... 1761 L L C P L Y G A L S L N D Q R K A I L P A P Q G M R K V V L Query_1 922 TTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTG 1011 0 721 .......................................................................................... 810 0 1920 ........... 1930 2 578 ........... 568 A T N I A E T S L T I E G I R L V V D C A Q E R V A R F D P Query_1 1012 GCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCG 1101 0 811 .......................................................................................... 900 R T G L T R L I T Q R V S Q A S M T Q R A G R A G R L E P G Query_1 1102 CGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGT 1191 0 901 .......................................................................................... 990 I S L H L I A K E Q A E R A A A Q S E P E I L Q S D L S G L Query_1 1192 ATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTG 1281 0 991 .......................................................................................... 1080 0 1039 ............ 1028 L M E L L Q W G C S D P A Q M S W L D Q P P V V N L L A A K Query_1 1282 CTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAA 1371 0 1081 .......................................................................................... 1170 R L L Q M L G A L E G E R L S A Q G Q K M A A L G N D P R L Query_1 1372 CGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGTGCGCAAGGGCAAAAAATGGCAGCGCTGGGTAACGATCCGCGTTTA 1461 0 1171 .......................................................................................... 1260 0 1973 ............ 1984 2 525 ............ 514 A A M L V S A K N D D E A A T A A K I A A I L E E P P R M G Query_1 1462 GCGGCAATGCTGGTTAGCGCGAAGAACGACGACGAAGCTGCTACCGCGGCAAAAATTGCCGCCATTCTCGAAGAGCCGCCACGGATGGGC 1551 0 1261 .......................................................................................... 1350 N S D L G V A F S R N Q P A W Q Q R S Q Q L L K R L N V R G Query_1 1552 AATAGTGACCTGGGCGTGGCGTTTTCGCGCAATCAACCAGCCTGGCAGCAACGTAGTCAGCAACTGTTAAAACGCTTAAACGTACGTGGC 1641 0 1351 .......................................................................................... 1440 G E A D S S L I A P L L A G A F A D R I A R R R G Q D Query_1 1642 GGTGAGGCAGACAGTTCGCTTATCGCGCCGCTACTTGCCGGGG-CGTTTGCCGA-TCG-CATTGCTCGTCGCC-GTGG-GCAAG--ATGG 1724 0 1441 ...........................................-..........-...-..............-....-.....--.... 1523 2 1032 ..A.......N....GC....G...N...N...G...N..A....A....AGA...N 976 G R Y Q L A N G M G A M L D A N D A L S R H E W L I A P L L Query_1 1725 ACGCTATCAACTGGC-AAACGGCATGGGAGCGATGCTCGATGCCAACGACGCGCTAAGCCGCCACGAATGGTTGATCGCACC-GTTATTA 1812 0 1524 ...............-..................................................................-....... 1611 2 975 ....N....-.....N.....T...T......................N..N.N.............-...........N..C....... 888 L Q G S A S P D A R I L L A L L V D I D E L V Q R C P Q L Query_1 1813 TTGCAGGGCAGCGCCTCGCCGGATGCGCGGATTTTACTGGCGCTGCTGGTCGATATTGATGAGTTA-GTACAACGCTGCCCGCAGCTGGT 1901 0 1612 ..................................................................-....................... 1700 2 887 ..................................................................A....................... 798 V Q Q S D T V E W D D A Q G T L K A W R R L Q I G Q L T V K Query_1 1902 ACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGT 1991 0 1701 .......................................................................................... 1790 0 658 ........... 668 2 797 ...................................................-...................................... 709 V Q P L A K P S E D E L H Q A M L N G I R D K G L S V L N W Query_1 1992 GCAGCCGCTGGCGAAACCGTCAGAA-GACGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGA 2080 0 1791 .........................-................................................................ 1879 2 708 .........................A..............................................................N. 619 T A E A E Q L R L R L L C A A K W L P E Y D W P A V D D E S Query_1 2081 CGGCGGAAGCGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGCCAGCGGTTGATGATGAAAGTT 2170 0 1880 .......................................................................................... 1969 0 796 ........... 806 0 2003 ........... 2013 2 618 ......................................N................................................... 529 L L A A L E T W L L P H M T G V H S L R G L K S L D I Y Q A Query_1 2171 TATTGGCAGCGCTGGAAACGTGGCTGCTGCCACATATGACTGGCGTACATTCACTACGCGGCCTGAAATCACTCGACATTTATCAGGCAC 2260 0 1970 .......................................................................................... 2059 0 1232 ............ 1243 0 1938 ........... 1948 2 528 ........................................C.....G........................................... 439 2 560 ........... 550 L R G L L D W G M Q Q R L D S E L P A H Y T V P T G S R I A Query_1 2261 TACGCGGATTACTTGATTGGGGAATGCAGCAACGTCTGGATAGTGAATTGCCTGCGCATTACACTGTGCCGACGGGAAGCCGGATCGCCA 2350 0 2060 .......................................................................................... 2149 2 438 .......................................................................................... 349 I R Y H E D N P P A L A V R M Q E M F G E A T N P T I A Q G Query_1 2351 TTCGTTATCATGAAGATAACCCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGC 2440 0 2150 .......................................................................................... 2239 2 348 ..............................................G...........T............................... 259 R V P L V L E L L S P A Q R P L Q I T R D L S D F W K G A Y Query_1 2441 GCGTGCCGCTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACC 2530 0 2240 .......................................................................................... 2329 2 258 .......................................................................................... 169 R E V Q K E M K G R Y P K H V W P D D P A N T A P T R R T K Query_1 2531 GTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAA 2620 0 2330 .......................................................................................... 2419 2 168 .......................................................................................... 79 K Y S * H W K W I T D P N S S S V D K L A A A L E H H H H H Query_1 2621 AGTATTCGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccacc 2710 0 2420 ........ 2427 2 78 ............................ 51 H * D P A A N K A R K E A E L A A A T A Query_1 2711 acTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGA 2771 0 1990 ............. 2002 0 607 ........... 617 2 49 ................. 33 2 508 ............. 496 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 9683600 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 3,491 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96147.102 (IDP96147.1-481.F..pMCSG68)
MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLASALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTPGENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGVFDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMTTGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAKIAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQATLTSNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDVSWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFLDSGLLQECQQEHQQVTDTQPYHCPIPKNVTPSPLK
ATGCAGGAAATCTATCGTTTTATCGACGATGCGATTGAAGCCGATCGCCAACGTTATACCGATATTGCCGATCAAATCTGGGATCATCCAGAAACACGTTTTGAAGAGTTCTGGTCAGCGGAGCATCTGGCTTCGGCGCTGGAATCTGCAGGCTTCACCGTTACCCGCAACGTAGGCAATATCCCAAATGCCTTTATTGCTTCGTTTGGTCAAGGCAAACCGGTTATCGCCCTGCTGGGAGAATATGACGCCCTGGCAGGTTTAAGTCAGCAAGCAGGTTGCGCGCAACCTACATCCGTGACGCCCGGTGAAAATGGTCACGGTTGCGGACACAATTTGCTGGGAACCGCCGCCTTTGCCGCTGCAATAGCCGTCAAGAAATGGCTGGAACAATATGGGCAAGGCGGCACGGTGCGCTTTTATGGTTGTCCTGGCGAAGAAGGCGGCTCGGGTAAAACGTTCATGGTTCGCGAGGGGGTATTTGATGATGTGGATGCGGCACTCACCTGGCACCCGGAAGCCTTTGCCGGTATGTTCAATACCCGCACGCTGGCAAACATTCAGGCATCATGGCGCTTTAAAGGGATCGCAGCACATGCCGCGAATTCCCCTCATTTGGGACGCAGCGCCCTTGATGCCGTAACGTTGATGACCACTGGCACCAACTTCCTCAACGAACATATTATTGAAAAAGCGCGCGTACACTATGCCATCACAAATAGCGGCGGGATCTCGCCCAACGTGGTCCAGGCGCAGGCAGAAGTGCTTTATCTTATCCGCGCCCCCGAAATGACCGACGTGCAGCATATTTATGATCGGGTCGCCAAAATCGCCGAAGGTGCGGCATTGATGACCGAAACCACGGTTGAATGCCGCTTCGACAAAGCCTGTTCCAGTTATCTCCCGAATCGCACCTTAGAAAATGCCATGTACCAGGCCCTATCCCATTTTGGTACCCCGGAATGGAACTCCGAAGAACTGGCTTTTGCGAAACAAATTCAGGCTACGCTCACCTCCAACGATCGGCAAAACAGTCTGAATAATATCGCCGCAACCGGTGGCGAAAACGGCAAGGTTTTTGCACTACGTCATCGTGAAACGGTACTGGCGAATGAAGTCGCTCCATATGCCGCCACCGATAACGTGCTTGCGGCATCGACTGATGTCGGCGACGTCAGTTGGAAACTGCCTGTTGCCCAGTGTTTCAGCCCCTGTTTTGCCGTCGGTACACCGCTACATACGTGGCAACTGGTTAGCCAGGGGCGAACATCTATTGCTCATAAAGGAATGCTGCTGGCGGCGAAAACTATGGCAGCAACCACAGTCAATCTCTTCCTTGATTCAGGGCTATTGCAAGAATGCCAACAAGAGCATCAGCAAGTAACGGACACGCAACCGTATCACTGCCCTATCCCGAAAAACGTGACACCGTCACCTTTAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,283 total letters Query= IDP96147.102 Length=1848 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2603 0.0 2_08A_A2_IDP96147.102_T7term.seq 1341 0.0 M Q E I Y R F I D D A I E A D R Q R Y T D I A D Q I W D H P Query_1 202 ATGCAGGAAATCTATCGTTTTATCGACGATGCGATTGAAGCCGATCGCCAACGTTATACCGATATTGCCGATCAAATCTGGGATCATCCA 291 0 1 .......................................................................................... 90 E T R F E E F W S A E H L A S A L E S A G F T V T R N V G N Query_1 292 GAAACACGTTTTGAAGAGTTCTGGTCAGCGGAGCATCTGGCTTCGGCGCTGGAATCTGCAGGCTTCACCGTTACCCGCAACGTAGGCAAT 381 0 91 .......................................................................................... 180 I P N A F I A S F G Q G K P V I A L L G E Y D A L A G L S Q Query_1 382 ATCCCAAATGCCTTTATTGCTTCGTTTGGTCAAGGCAAACCGGTTATCGCCCTGCTGGGAGAATATGACGCCCTGGCAGGTTTAAGTCAG 471 0 181 .......................................................................................... 270 Q A G C A Q P T S V T P G E N G H G C G H N L L G T A A F A Query_1 472 CAAGCAGGTTGCGCGCAACCTACATCCGTGACGCCCGGTGAAAATGGTCACGGTTGCGGACACAATTTGCTGGGAACCGCCGCCTTTGCC 561 0 271 .......................................................................................... 360 0 291 ................ 276 A A I A V K K W L E Q Y G Q G G T V R F Y G C P G E E G G S Query_1 562 GCTGCAATAGCCGTCAAGAAATGGCTGGAACAATATGGGCAAGGCGGCACGGTGCGCTTTTATGGTTGTCCTGGCGAAGAAGGCGGCTCG 651 0 361 .......................................................................................... 450 G K T F M V R E G V F D D V D A A L T W H P E A F A G M F N Query_1 652 GGTAAAACGTTCATGGTTCGCGAGGGGGTATTTGATGATGTGGATGCGGCACTCACCTGGCACCCGGAAGCCTTTGCCGGTATGTTCAAT 741 0 451 .......................................................................................... 540 T R T L A N I Q A S W R F K G I A A H A A N S P H L G R S A Query_1 742 ACCCGCACGCTGGCAAACATTCAGGCATCATGGCGCTTTAAAGGGATCGCAGCACATGCCGCGAATTCCCCTCATTTGGGACGCAGCGCC 831 0 541 .......................................................................................... 630 L D A V T L M T T G T N F L N E H I I E K A R V H Y A I T N Query_1 832 CTTGATGCCGTAACGTTGATGACCACTGGCACCAACTTCCTCAACGAACATATTATTGAAAAAGCGCGCGTACACTATGCCATCACAAAT 921 0 631 .......................................................................................... 720 S G G I S P N V V Q A Q A E V L Y L I R A P E M T D V Q H Query_1 922 AGCGGCGGGATCTCGCCCAACGTGGTCCAGGCGCAGGCA-GAAGTGCTTTATCTTATCCGCGCCCCCGAAA-TGACCGACGTGCAGCATA 1009 0 721 .......................................-...............................-.................. 808 2 831 ..G........A..-.............-.......A...............................A......N........... 747 I Y D R V A K I A E G A A L M T E T T V E C R F D K A C S Query_1 1010 TTTATGATCGGG-TCGCCAAAATCGCCGAAGGTGCGGCATTGATGACCGAAA-CCACGGTTGAATGCCGCTTCGACAAAGCCTGTTCCAG 1097 0 809 ............-.......................................-..................................... 896 2 746 ..-.....N...G.............N.........................A...........NN...............N........ 658 S Y L P N R T L E N A M Y Q A L S H F G T P E W N S E E L A Query_1 1098 TTATCTCCCGAATCGCACCTTAGAAAATGCCA-TGTACCAGGCCCTATCCCATTTTGGTACCCCGGAATGGAACTCCGAAGAACTGGCTT 1186 0 897 ................................-......................................................... 985 2 657 ................................A......GA................................................. 568 F A K Q I Q A T L T S N D R Q N S L N N I A A T G G E N G K Query_1 1187 TTGCGAAACAAATTCAGGCTACGCTCACCTCCAACGATCGGCAAAACAGTCTGAATAATATCGCCGCAACCGGTGGCGAAAACGGCAAGG 1276 0 986 .......................................................................................... 1075 0 1222 ........... 1212 2 567 .......................................................................................... 478 2 331 ........... 341 V F A L R H R E T V L A N E V A P Y A A T D N V L A A S T D Query_1 1277 TTTTTGCACTACGTCATCGTGAAACGGTACTGGCGAATGAAGTCGCTCCATATGCCGCCACCGATAACGTGCTTGCGGCATCGACTGATG 1366 0 1076 .......................................................................................... 1165 2 477 ........N...................................................................A........A.... 388 V G D V S W K L P V A Q C F S P C F A V G T P L H T W Q L V Query_1 1367 TCGGCGACGTCAGTTGGAAACTGCCTGTTGCCCAGTGTTTCAGCCCCTGTTTTGCCGTCGGTACACCGCTACATACGTGGCAACTGGTTA 1456 0 1166 .......................................................................................... 1255 0 1034 ........... 1024 2 387 .......................................................................................... 298 2 519 ........... 529 S Q G R T S I A H K G M L L A A K T M A A T T V N L F L D S Query_1 1457 GCCAGGGGCGAACATCTATTGCTCATAAAGGAATGCTGCTGGCGGCGAAAACTATGGCAGCAACCACAGTCAATCTCTTCCTTGATTCAG 1546 0 1256 .......................................................................................... 1345 2 297 .......................................................................................... 208 G L L Q E C Q Q E H Q Q V T D T Q P Y H C P I P K N V T P S Query_1 1547 GGCTATTGCAAGAATGCCAACAAGAGCATCAGCAAGTAACGGACACGCAACCGTATCACTGCCCTATCCCGAAAAACGTGACACCGTCAC 1636 0 1346 .......................................................................................... 1435 2 207 .......................................................................................... 118 P L K * H W K W I T D P N S S S V D K L A A A L E H H H H H Query_1 1637 CTTTAAAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTC-GAGcaccaccaccaccac 1725 0 1436 ........ 1443 2 117 .......................................................................G.................. 28 H * D P A A Query_1 1726 cacTGAGATCC-GGCTGCT 1743 2 27 --T........G....... 11 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4094520 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Oct 26, 2017 10:15 AM Number of letters in database: 2,283 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2