Clone ID: CPX300511.102 (CPX300511.1-289.F..pMCSG68)
MSILVNKNTKVIVQGFTGKEATFHAEQCMAYGTNIVGGITPHKGGQTHLGKPVFDTVADAVKATKADVSLIFVPAFAVGDSVIEAADAGIKLAVVITEHTPVKDMMFAKQYANKKGMKIIGPNCPGIITSEECKLGIMPGFIFKKGCVGLISKSGTLTYEAANQVVQGGYGISTAVGIGGDPIIGLAYKELLSEFQKDDETKAIVMIGEIGGSLEVEAAKFIKENISKPVVAFIAGATAPKGKRMGHAGAIVGSADESAAAKKEALKSYGIHVVDSPALIGEEIQKILG
ATGAGTATATTGGTTAATAAAAATACAAAAGTAATAGTTCAAGGTTTTACAGGTAAAGAAGCAACTTTTCATGCCGAGCAATGCATGGCTTATGGTACAAATATAGTCGGAGGCATCACTCCTCATAAAGGTGGACAAACTCATTTAGGTAAACCAGTTTTTGATACCGTTGCAGATGCGGTTAAAGCAACAAAGGCTGACGTGAGCTTGATCTTTGTACCTGCTTTTGCTGTAGGTGATAGCGTGATTGAAGCAGCTGATGCGGGTATTAAGCTTGCAGTTGTAATCACCGAGCATACGCCTGTAAAGGATATGATGTTTGCTAAGCAATATGCTAATAAAAAGGGTATGAAAATCATTGGACCAAATTGTCCAGGTATTATCACTTCTGAAGAATGTAAGTTAGGTATTATGCCAGGTTTTATTTTTAAAAAAGGTTGCGTGGGGCTTATTTCAAAAAGTGGAACTTTAACTTATGAAGCTGCTAATCAGGTAGTTCAAGGTGGTTATGGAATTTCTACTGCAGTAGGAATTGGTGGTGATCCTATCATAGGTCTTGCTTATAAAGAACTTTTAAGTGAATTCCAAAAAGATGATGAAACTAAAGCTATTGTAATGATAGGTGAAATTGGTGGTAGTTTGGAAGTGGAAGCGGCTAAATTTATTAAAGAAAATATTAGCAAACCTGTGGTAGCATTCATTGCAGGTGCAACTGCTCCTAAAGGCAAAAGAATGGGGCATGCAGGTGCCATAGTAGGAAGTGCTGATGAAAGTGCTGCAGCTAAAAAAGAAGCTTTAAAAAGCTATGGAATTCATGTGGTGGATTCTCCGGCTTTAATTGGTGAAGAAATTCAAAAAATATTAGGT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,320 total letters Query= CPX300511.102 Length=2445 Score E Sequences producing significant alignments: (Bits) Value 1_03E_E3_CPX300511.102_T7S.seq 1932 0.0 2_09E_E3_CPX300511.102_T7term.seq 1911 0.0 0_wt 1564 0.0 Q F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 55 CAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTG 144 1 2 ................-......................................................................... 90 1 481 ........... 491 W S H P Q F E K G T E N L Y F Q S N A M N I H E Y Q A K A I Query_1 145 TGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGAACATACATGAGTATCAAGCAAAAGCAATT 234 1 91 .......................................................................................... 180 F V D N G I P T L K G K V A F S V D E A V A N A K E L G G S Query_1 235 TTTGTAGACAATGGCATCCCTACCTTAAAGGGAAAAGTTGCATTTAGCGTAGATGAAGCTGTGGCTAATGCTAAAGAATTAGGCGGTAGT 324 1 181 ...................................G...................................................... 270 V W A V K A Q I H A G G R G L G G G V K I A K N L D E V K D Query_1 325 GTTTGGGCAGTTAAAGCTCAAATTCATGCAGGAGGCCGTGGTCTTGGTGGTGGTGTTAAAATCGCTAAGAATTTAGATGAGGTAAAAGAT 414 1 271 .......................................................................................... 360 2 53 .....GG........... 70 Y A S K I L G M N L V T H Q T G P E G K L V Q K L Y I E S G Query_1 415 TATGCAAGTAAAATTTTAGGAATGAATTTAGTAACTCATCAAACAGGACCTGAAGGCAAACTTGTGCAAAAACTTTATATAGAAAGTGGT 504 1 361 .......................................................................................... 450 A N I V K E Y Y L A I L F N R M A E Q I T I I A S S E G G M Query_1 505 GCTAATATAGTAAAAGAGTATTATTTGGCTATTTTGTTTAATAGAATGGCAGAGCAAATTACCATTATCGCTTCAAGTGAAGGAGGGATG 594 1 451 .......................................................................................... 540 1 20 ........... 30 D I E K V A K E S P E K I A K V G I D P Q I G F K M F H G L Query_1 595 GATATAGAAAAAGTAGCCAAAGAAAGTCCTGAAAAAATTGCAAAAGTAGGTATTGATCCACAAATTGGATTTAAAATGTTTCATGGACTT 684 1 541 .......................................................................................... 630 E V A R V L G L D K D E G K K L I S M I A K L Y K L Y M D K Query_1 685 GAAGTAGCGAGAGTTTTGGGACTTGATAAAGATGAAGGCAAAAAACTCATTTCTATGATAGCTAAACTTTATAAGCTTTACATGGATAAA 774 1 631 .......................................................................................... 720 D M N M L E I N P L I K T A E G D F Y A L D A K C S F D D S Query_1 775 GATATGAATATGCTTGAAATTAATCCTTTAATTAAAACTGCAGAAGGGGATTTTTATGCCCTTGATGCAAAATGCAGTTTTGATGATAGT 864 1 721 .......................................................................................... 810 A L Y R H P E I A E L R D T T E E N P A E R E A A E F G L S Query_1 865 GCGCTTTATCGTCATCCTGAAATTGCTGAACTTAGAGATACTACTGAAGAAAATCCTGCTGAAAGAGAAGCGGCTGAATTTGGCTTAAGC 954 1 811 .......................................................................................... 900 Y V K L D G D V A C M V N G A G L A M A T M D I I N Y S G A Query_1 955 TATGTGAAATTAGATGGAGATGTAGCTTGTATGGTAAATGGTGCGGGTCTAGCAATGGCAACTATGGATATTATTAATTATAGTGGTGCA 1044 1 901 .......................................................................................... 990 K P A N F L D V G G G A S P E T V A K A F E I I L R D K Query_1 1045 AAACCTGCAAATTTCTTAGATGTAGGT-GGGGGAGCTTCTCCAGAAACTGTTGCAAAGGCTTTTGAAATTATTTTAAGAGATAAAAA--- 1130 1 991 ...........................G.....N......T............................NN........N.......AAT 1080 N V K V I F I N I F G G I V R C D R I A N G I L E A T K N V Query_1 1131 TGTAAAAGTTATATTTATCAATATCTTTGGTGGTATTGTTCG-TTGTGATAGGATAGCAAATGGAATTTTAGAAGCTACTAAGAATGTTG 1219 1 1081 G.....N..AT....N...-NN...N.NN..NNNN..NA...NNN.......NTA...-..............G.......--G.NNN.. 1166 2 1182 ........NN-...........-.....N.. 1154 E V N I P I V V R L D G T N A A E A K T I L D N S N L K N Query_1 1220 AGG-TAAATATTCCAATTGTAGTGCG-TCTTGATGGCACTAACGCAGCAGAAGCAAAGACAATTTTAGATAATTCAAATCTTAAAAATAT 1307 1 1167 ...-..... 1174 2 1153 ...G..........N-......N...G..C...N.NNNNNCG.-......................NN.NN................... 1066 I K A A T N L K N G A E L V K S L V G * G W K M S I L V N K Query_1 1308 CAAAGCAGCAACCAATCTTAAAAACGGTGCAGAGCTTGTAAAAAGTTTAGTAGGATAAGGATGGAAAATGAGTATATTGGTTAATAAAAA 1397 2 1065 ..........N.....N.--......-.........GT.........-...................-...................... 981 0 1 ....................... 23 N T K V I V Q G F T G K E A T F H A E Q C M A Y G T N I V G Query_1 1398 -TACAAAAGTAATAGTTCAAGGTTTTACAGGTAAAGAAGCAACTTTTCATGCCGAGCAATGCATGGCTTATGGTACAAATATAGTCGGAG 1486 2 980 A......-.................................................................................. 892 2 510 ........... 500 0 24 -......................................................................................... 112 0 494 ........... 504 G I T P H K G G Q T H L G K P V F D T V A D A V K A T K A D Query_1 1487 GCATCACTCCTCATAAAGGTGGACAAACTCATTTAGGTAAACCAGTTTTTGATACCGTTGCAGATGCGGTTAAAGCAACAAAGGCTGACG 1576 2 891 .......................................................................................... 802 0 113 .......................................................................................... 202 V S L I F V P A F A V G D S V I E A A D A G I K L A V V I T Query_1 1577 TGAGCTTGATCTTTGTACCTGCTTTTGCTGTAGGTGATAGCGTGATTGAAGCAGCTGATGCGGGTATTAAGCTTGCAGTTGTAATCACCG 1666 2 801 .......................................................................................... 712 0 203 .......................................................................................... 292 E H T P V K D M M F A K Q Y A N K K G M K I I G P N C P G I Query_1 1667 AGCATACGCCTGTAAAGGATATGATGTTTGCTAAGCAATATGCTAATAAAAAGGGTATGAAAATCATTGGACCAAATTGTCCAGGTATTA 1756 2 711 .......................................................................................... 622 0 293 .......................................................................................... 382 I T S E E C K L G I M P G F I F K K G C V G L I S K S G T L Query_1 1757 TCACTTCTGAAGAATGTAAGTTAGGTATTATGCCAGGTTTTATTTTTAAAAAAGGTTGCGTGGGGCTTATTTCAAAAAGTGGAACTTTAA 1846 2 621 .......................................................................................... 532 0 383 .......................................................................................... 472 T Y E A A N Q V V Q G G Y G I S T A V G I G G D P I I G L A Query_1 1847 CTTATGAAGCTGCTAATCAGGTAGTTCAAGGTGGTTATGGAATTTCTACTGCAGTAGGAATTGGTGGTGATCCTATCATAGGTCTTGCTT 1936 2 531 .......................................................................................... 442 2 470 ....C............A.... 491 2 969 ........... 959 0 473 .......................................................................................... 562 0 534 ....C............A.... 513 0 35 ........... 45 Y K E L L S E F Q K D D E T K A I V M I G E I G G S L E V E Query_1 1937 ATAAAGAACTTTTAAGTGAATTCCAAAAAGATGATGAAACTAAAGCTATTGTAATGATAGGTGAAATTGGTGGTAGTTTGGAAGTGGAAG 2026 2 441 .......................................................................................... 352 2 131 ........... 121 0 563 .......................................................................................... 652 A A K F I K E N I S K P V V A F I A G A T A P K G K R M G H Query_1 2027 CGGCTAAATTTATTAAAGAAAATATTAGCAAACCTGTGGTAGCATTCATTGCAGGTGCAACTGCTCCTAAAGGCAAAAGAATGGGGCATG 2116 2 351 .......................................................................................... 262 0 653 .......................................................................................... 742 A G A I V G S A D E S A A A K K E A L K S Y G I H V V D S P Query_1 2117 CAGGTGCCATAGTAGGAAGTGCTGATGAAAGTGCTGCAGCTAAAAAAGAAGCTTTAAAAAGCTATGGAATTCATGTGGTGGATTCTCCGG 2206 2 261 .......................................................................................... 172 0 743 .......................................................................................... 832 A L I G E E I Q K I L G * H W K W I T D P N S S S V D K L A Query_1 2207 CTTTAATTGGTGAAGAAATTCAAAAAATATTAGGTTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGG 2296 2 171 .......................................................................................... 82 2 364 ........... 354 0 833 ................................... 867 0 640 ........... 650 A A L E H H H H H H * D P A A N K A R Query_1 2297 CCGCACTCGAGcaccaccaccaccaccacTGAG-ATCCGGCTGCTAACAAAG-CCCGAAA 2354 2 81 .................................A..................C....... 22 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 7937132 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 3,320 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CPX300512.102 (CPX300512.1-290.F..pMCSG68)
MSVLVDKNTKVLVQGFTGKNGTFHSEQAIAYGTNIVGGVTPGKGGTTHLDRPVFNTMAEAVAATGADASVIYVPAPFVKDSAIEAIDSGVKLVVIITEGVPTLDMLVVKEYLKDKDVRVIGPNCPGIITPGECKIGIMPGHIHMKGKVGIISRSGTLTYEAVAQTTKLGFGQSTCIGIGGDPIPGMNQIEALKLLENDPQTEAIILIGEIGGTAEEEAAEYIKHNVTKPVIGYIAGVTAPPGKRMGHAGAIISGGKGTAEEKFAAFEAAGIAYTRSPAEIGKKLKEVTGW
ATGAGCGTATTAGTTGATAAAAATACAAAAGTTTTAGTACAGGGTTTTACTGGTAAAAATGGTACTTTCCATTCAGAACAAGCTATTGCTTATGGTACAAATATTGTAGGTGGCGTAACTCCTGGTAAAGGTGGCACTACTCACTTAGATAGACCAGTGTTCAATACAATGGCTGAAGCTGTAGCAGCTACTGGTGCTGATGCGTCTGTAATCTATGTTCCAGCTCCATTTGTAAAAGACTCTGCAATTGAAGCGATCGATTCTGGTGTTAAGCTAGTAGTAATCATTACAGAAGGTGTCCCTACTCTAGATATGCTAGTAGTTAAAGAGTACTTAAAAGATAAAGATGTGAGAGTAATTGGTCCTAACTGTCCAGGGATCATCACTCCAGGTGAGTGTAAGATTGGTATTATGCCGGGTCATATTCATATGAAAGGTAAAGTTGGTATCATTTCTCGTTCTGGTACTTTAACTTACGAAGCAGTTGCTCAAACTACTAAACTAGGCTTTGGTCAATCTACTTGTATTGGTATAGGCGGAGACCCTATCCCAGGTATGAACCAAATCGAAGCTCTTAAACTTTTAGAGAATGACCCACAAACTGAAGCTATCATCTTAATCGGTGAAATTGGTGGTACAGCTGAAGAAGAAGCTGCTGAATATATCAAGCACAATGTAACTAAGCCTGTTATTGGATATATCGCTGGTGTTACAGCTCCTCCAGGAAAGCGTATGGGTCATGCTGGTGCTATTATCTCTGGTGGTAAAGGTACCGCTGAAGAAAAATTTGCCGCATTCGAAGCTGCTGGTATTGCTTATACTAGATCACCTGCTGAGATCGGCAAGAAGCTTAAAGAAGTTACTGGCTGG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,344 total letters Query= CPX300512.102 Length=2470 Score E Sequences producing significant alignments: (Bits) Value 1_03F_F3_CPX300512.102_T7S.seq 1959 0.0 2_09F_F3_CPX300512.102_T7term.seq 1896 0.0 0_wt 1570 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 56 AATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGT 145 1 2 ...............-.......................................................................... 90 W S H P Q F E K G T E N L Y F Q S N A M N L H E Y Q A K D L Query_1 146 GGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGAACTTACATGAATATCAAGCTAAAGATCTTT 235 1 91 .......................................................................................... 180 L E S Y G L K V Q K G I V A H N P N E A A Q A F D Q L G G K Query_1 236 TAGAAAGCTATGGTCTAAAAGTTCAAAAAGGTATCGTAGCTCATAACCCAAATGAAGCTGCTCAAGCATTTGACCAATTAGGTGGCAAAT 325 1 181 .......................................................................................... 270 F A V V K A Q V H A G G R G K A G G V K V V K S S Q E A R E Query_1 326 TTGCTGTGGTTAAAGCTCAGGTACATGCTGGTGGTCGTGGTAAAGCTGGTGGTGTAAAGGTTGTTAAATCATCTCAAGAAGCTCGTGAAG 415 1 271 ................................................................................A......... 360 1 625 ............ 636 V A E S L I G K N L V T F Q T D A E G Q P V N S V G V F E D Query_1 416 TAGCAGAAAGCTTAATCGGTAAAAATCTTGTTACTTTCCAAACTGATGCTGAAGGTCAGCCAGTTAATTCTGTAGGTGTATTTGAAGATG 505 1 361 .......................................................................................... 450 1 637 .... 640 V Y P V T R E L Y L G A V V D R S S R K V T F M A S T E G G Query_1 506 TATACCCTGTTACTCGTGAGTTATACCTTGGTGCGGTAGTTGATAGATCTAGCCGTAAAGTAACATTCATGGCTTCAACTGAAGGCGGTG 595 1 451 .......................................................................................... 540 V D I E E V A H N S P E K I L K V E V D P L V G L Q P F Q A Query_1 596 TAGATATTGAAGAAGTTGCACACAACTCTCCTGAAAAAATCCTTAAAGTAGAAGTAGATCCTTTAGTTGGCTTACAACCTTTCCAAGCTC 685 1 541 .......................................................................................... 630 1 349 ..A... 354 R E V A F K L G L E G K Q I N D F V K T M L G A Y K A F I E Query_1 686 GTGAAGTAGCTTTCAAGCTTGGTCTAGAAGGCAAGCAAATCAATGATTTTGTTAAAACTATGCTAGGCGCTTACAAAGCATTCATCGAAT 775 1 631 .......................................................................................... 720 1 355 .......... 364 C D F A L F E I N P L A V R E N G E I V C V D G K I N L D S Query_1 776 GTGATTTTGCCCTATTTGAAATCAACCCTCTTGCAGTTAGAGAAAACGGAGAGATTGTCTGTGTTGATGGTAAAATCAACCTTGATTCAA 865 1 721 .......................................................................................... 810 N A L Y R H P K L L A L R D K S Q E N A K E L K A S E H E L Query_1 866 ATGCTCTTTATAGACACCCTAAACTTTTAGCTCTAAGAGACAAATCTCAAGAAAATGCTAAAGAACTTAAAGCTTCAGAGCATGAGCTAA 955 1 811 .......................................................................................... 900 1 341 ........... 351 2 430 ........... 420 0 576 ........... 586 N Y V A L E G N I G C M V N G A G L A M A T M D I I Q L Y Query_1 956 ACTATGTAGCTCTTGAAGGTAACATCGGTTGTATGGTAAACGG-TGCTGGTCTAGCAATGGCTACTATGGATATTATCCAATTATAC-GG 1043 1 901 ...........................................N...........................................G.. 990 G G K P A N F L D V G G G A T K E R V I E A F K L I L D D E Query_1 1044 TGGTAAGCCTGCTAACTTCCTAGATGTAGGTGGTGGTGCTACTAAAGAGAGAGTAATCGAAGCTTTCAAACTAATCCTAGATGATGAAAA 1133 1 991 .............-......................N...........N..........................-.N............ 1078 2 60 ............ 71 N V K A I L I N I F G G I V R C D M I A E A I I E A V K E Query_1 1134 TGTAAAAGCTATTTTAATTAACATCTT--CGGTGGTA-TTGTTCGTTGTGATATGATCGCTG-AAGCAATTATTGAAGCTGTTAAAGAAG 1219 1 1079 ...........................TCG......NT..NNN..NN....N..........A.....NNN....G.ANNN.NNN.N... 1168 V N V T V P V V V R L E G N N A E K G A K I L A D S G L K Query_1 1220 -TAAATGT-AACTG-TACCAGTAGTTGTTCGTCTAGAAGGTAACAATGCAGAAAAA-GGTGCTAAAATATTAGCTGATTCAGGTTTAAAA 1305 1 1169 N.....NNA.....N....... 1190 2 1163 ...-.TNN......-......N...NNTN---.C...........A.........-.........N.NNNNT----... 1095 L I P A D G L A D A A D K V V K S L G * * I K T V K R I L N Query_1 1306 CTAATACCTGCTGATGGTTTAGCTGATGCTGCTGATAAAGTTGTGAAATCACTAGGTTAATAAATTAAAACAGTTAAAAGGATATTAAAT 1395 2 1094 ......N-.......NN..-.................................................-..................N. 1008 N E R I S * * K Y K S F S T G F Y W * K W Y F P F R T S Y Query_1 1396 AATGAGCGTATTAGTTGATAAAAA-TACAAAAGTTTTAGTACAGGGTTTTACTGGTAAAAATGGTACTTTCCATTCAGAACAAGCTATTG 1484 2 1007 ........................A............................................C.................... 918 0 1 .......................-................................................................. 88 C L W Y K Y C R W R N S W * R W H Y S L R * T S V Q Y N G * Query_1 1485 CTTATGGTACAAATATTGTAGGTGGCGTAACTCCTGGTAAAGGTGGCACTACTCACTTAGATAGACCAGTGTTCAATACAATGGCTGAAG 1574 2 917 .......................................................................................... 828 0 89 .......................................................................................... 178 S C S S Y W C * C V C N L C S S S I C K R L C N * S D R F W Query_1 1575 CTGTAGCAGCTACTGGTGCTGATGCGTCTGTAATCTATGTTCCAGCTCCATTTGTAAAAGACTCTGCAATTGAAGCGATCGATTCTGGTG 1664 2 827 ...........................................................................T.............. 738 0 179 .......................................................................................... 268 C * A S S N H Y R R C P Y S R Y A S S * R V L K R * R C E S Query_1 1665 TTAAGCTAGTAGTAATCATTACAGAAGGTGTCCCTACTCTAGATATGCTAGTAGTTAAAGAGTACTTAAAAGATAAAGATGTGAGAGTAA 1754 2 737 .......................................................................................... 648 0 269 .......................................................................................... 358 N W S * L S R D H H S R * V * D W Y Y A G S Y S Y E R * S W Query_1 1755 TTGGTCCTAACTGTCCAGGGATCATCACTCCAGGTGAGTGTAAGATTGGTATTATGCCGGGTCATATTCATATGAAAGGTAAAGTTGGTA 1844 2 647 .......................................................................................... 558 0 359 .......................................................................................... 448 Y H F S F W Y F N L R S S C S N Y * T R L W S I Y L Y W Y R Query_1 1845 TCATTTCTCGTTCTGGTACTTTAACTTACGAAGCAGTTGCTCAAACTACTAAACTAGGCTTTGGTCAATCTACTTGTATTGGTATAGGCG 1934 2 557 .......................................................................................... 468 0 449 .......................................................................................... 538 R R P Y P R Y E P N R S S * T F R E * P T N * S Y H L N R * Query_1 1935 GAGACCCTATCCCAGGTATGAACCAAATCGAAGCTCTTAAACTTTTAGAGAATGACCCACAAACTGAAGCTATCATCTTAATCGGTGAAA 2024 1 830 ........... 840 2 467 .......................................................................................... 378 0 539 .......................................................................................... 628 N W W Y S * R R S C * I Y Q A Q C N * A C Y W I Y R W C Y S Query_1 2025 TTGGTGGTACAGCTGAAGAAGAAGCTGCTGAATATATCAAGCACAATGTAACTAAGCCTGTTATTGGATATATCGCTGGTGTTACAGCTC 2114 2 377 .......................................................................................... 288 0 629 .......................................................................................... 718 S S R K A Y G S C W C Y Y L W W * R Y R * R K I C R I R S C Query_1 2115 CTCCAGGAAAGCGTATGGGTCATGCTGGTGCTATTATCTCTGGTGGTAAAGGTACCGCTGAAGAAAAATTTGCCGCATTCGAAGCTGCTG 2204 2 287 .......................................................................................... 198 0 719 .......................................................................................... 808 W Y C L Y * I T C * D R Q E A * R S Y W L V T L E V D N G S Query_1 2205 GTATTGCTTATACTAGATCACCTGCTGAGATCGGCAAGAAGCTTAAAGAAGTTACTGGCTGGTAACATTGGAAGTGGATAACGGATCCGA 2294 2 197 .......................................................................................... 108 0 809 .............................................................. 870 E F E L R R Q A C G R T R A P P P P P L R S G C * Q S P K Query_1 2295 ATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCT-AACAAAGCCC--GAAAGG 2381 2 107 .......................................................................A..........CGA..... 18 G Query_1 2382 AA 2383 2 17 .. 16 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 8077729 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 3,344 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: CPX300513.102 (CPX300513.1-300.F..pMCSG68)
MSVLVNKDTKVIVQGITGSQGLFHTKQMIEYGTKIVGGVTPGKGGTDIEGVPVFDTVEDAVKATGANASVVYVPPAFAADAIMEAVDAEIDLVVCITEGIPVLDMVNVKRYMAGKHTRLLGPNCPGVITPDECKIGIMPGYIHKKGHVGIVSRSGTLTYEAVHQLTQEGIGQSTAVGIGGDPVNGTDFIDALKAFNEDEETHAVIMIGEIGGTAEEEAAEWVKANMTKPVVGFIGGQTAPAGKRMGHAGAIISGGKGTAAEKIKTMEACGIKVAETPAVMGETLISVLKEKGLFETCKNY
ATGAGCGTATTAGTTAATAAAGATACAAAAGTTATTGTTCAAGGTATTACAGGTTCTCAAGGATTATTCCACACAAAACAAATGATTGAATACGGTACGAAAATTGTCGGTGGTGTAACGCCAGGTAAAGGTGGCACTGACATTGAAGGTGTACCAGTATTTGATACTGTAGAAGATGCAGTGAAAGCAACAGGCGCAAACGCTTCAGTTGTATACGTTCCACCCGCTTTTGCGGCTGATGCAATTATGGAAGCAGTTGATGCAGAAATTGATTTAGTAGTATGTATTACCGAAGGAATTCCTGTATTAGATATGGTAAACGTAAAGAGATATATGGCTGGTAAACATACACGTTTACTTGGACCAAACTGCCCAGGTGTTATTACGCCTGATGAATGTAAAATCGGTATTATGCCAGGATACATTCATAAAAAAGGTCATGTAGGTATCGTATCTCGCTCTGGTACATTAACGTATGAGGCTGTACATCAGTTAACACAAGAAGGTATTGGCCAATCTACTGCTGTAGGTATCGGCGGAGACCCAGTTAACGGTACAGACTTTATTGATGCATTAAAAGCATTTAATGAAGATGAAGAAACACATGCTGTAATTATGATTGGTGAAATCGGTGGTACAGCGGAAGAAGAGGCAGCTGAATGGGTGAAAGCTAATATGACAAAACCTGTTGTAGGCTTCATTGGTGGCCAAACAGCGCCTGCTGGCAAACGTATGGGCCATGCTGGTGCAATCATTTCTGGCGGTAAAGGAACTGCTGCAGAAAAGATTAAAACGATGGAAGCTTGTGGTATTAAAGTAGCGGAAACTCCAGCAGTTATGGGAGAAACGCTAATCTCTGTCTTAAAAGAAAAAGGTCTATTTGAGACTTGTAAAAACTAC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,634 total letters Query= CPX300513.102 Length=2486 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1624 0.0 2_09G_G3_CPX300513.102_T7term.seq 1559 0.0 1_03G_G3_CPX300513.102_T7S.seq 700 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V Query_1 60 CCCCTCT-AGAAATAA--TTTTGTTTAACTTT-AAGAAGGAGATATA-CATAT-GCACCATCAT-CATCAT-CATTCTTCTGG-TGTAG- 139 2 1089 .-....N...CA.A.....N 1071 1 9 ...N...T......T.AT..............T..N...........N.....T..........T......T...........-.....- 96 D L W S H P Q F E K G T E N L Y F Q S N A M N I H E Y Q G Query_1 140 ATCTGTGGTCTCATCCGCAGTTCGAAAAGGG-TACCG-AGAACC-TGTAC-TTCCAATCCAATGCCATGAATATCCATGAGTACCAAGGT 225 0 58 .-......-.... 48 2 1070 ..N.AN.N.N........N.G..........T....NA....N.A.....T....N...... 1009 1 97 ...............................-.....-......-.....-................... 162 1 216 .-......-.... 206 K A V L R S Y G V S V P N G K V A F T V E E A V E A A K E L Query_1 226 AAGGCAGTCCTTAGAAGTTATGGGGTTAGCGTTCCGAACGGGAAGGTTGCATTTACAGTAGAAGAAGCTGTAGAAGCAGCGAAAGAATTA 315 G T D V C V V K A Q I H A G G R G K A G G V K V A K N L D E Query_1 316 GGAACGGATGTATGTGTAGTAAAAGCACAAATTCACGCTGGTGGACGCGGCAAAGCTGGCGGAGTAAAAGTTGCAAAAAATTTAGATGAA 405 V R T Y A E S I L G T T L V T H Q T G P E G K E V K R L L I Query_1 406 GTTCGTACATATGCAGAGAGCATTTTAGGAACTACACTTGTGACACATCAAACAGGTCCTGAAGGTAAGGAAGTAAAACGCTTACTTATC 495 E E G C D I K K E Y Y V G L V L D R A T S Q V V L M A S E E Query_1 496 GAAGAAGGTTGCGATATTAAAAAAGAATATTATGTAGGTCTTGTTTTAGATCGTGCAACTTCTCAAGTTGTTTTAATGGCGTCTGAAGAA 585 G G T E I E E V A E K T P E K I F K E Y I D P A V G L Q G F Query_1 586 GGCGGAACAGAAATTGAAGAAGTAGCAGAAAAAACACCAGAAAAAATCTTTAAAGAATATATTGATCCAGCAGTAGGTTTACAAGGCTTC 675 Q A R R I A F N I N I P K E L V G Q A V K F M M G L Y R A F Query_1 676 CAAGCACGCCGGATTGCATTTAACATCAATATTCCAAAAGAGCTTGTAGGACAAGCTGTGAAGTTTATGATGGGCTTATACCGTGCGTTT 765 I E K D C S I A E I N P L V T T G D G K V M A L D A K L N F Query_1 766 ATCGAAAAAGATTGTTCTATCGCAGAGATTAATCCACTTGTTACAACAGGTGACGGTAAAGTTATGGCATTAGATGCGAAATTAAACTTT 855 D S N A L Y R H K D I L E L R D L D E E D A K E I E A S K Y Query_1 856 GATTCTAACGCTTTATATCGCCATAAAGACATTTTAGAACTTCGTGATCTTGATGAAGAAGATGCAAAAGAAATCGAAGCTTCTAAATAT 945 D L N Y I P L D G N I G C M V N G A G L A M A T M D I I K H Query_1 946 GACTTAAACTACATTCCTTTAGATGGAAATATCGGTTGTATGGTTAATGGTGCAGGTTTAGCGATGGCTACGATGGATATCATTAAACAT 1035 Y H G D P A N F L D V G G G A T A E K V T E A F K I I L S D Query_1 1036 TACCATGGCGATCCAGCTAACTTCTTAGATGTTGGTGGCGGTGCGACAGCTGAAAAAGTTACAGAAGCATTTAAAATTATCCTTTCTGAC 1125 K N V K G I F V N I F G G I M K C D V I A E G V I E A T K Q Query_1 1126 AAAAATGTAAAAGGTATCTTCGTTAACATTTTTGGTGGCATTATGAAGTGTGATGTTATTGCAGAAGGTGTTATTGAAGCAACGAAACAA 1215 V G L E L P L V V R L E G T N V E L G K K I L N E S G L N I Query_1 1216 GTAGGTCTTGAATTGCCTTTAGTTGTACGTCTTGAAGGTACAAACGTTGAGTTAGGTAAGAAAATTTTAAATGAGTCTGGCTTAAATATT 1305 V A A E S M T D G A Q K I V S L V G * * K A G E K K * A Y * Query_1 1306 GTTGCAGCAGAATCTATGACAGACGGTGCACAAAAAATTGTTTCACTAGTGGGCTAATAGAAAGCGGGGGAGAAAAAATGAGCGTATTAG 1395 0 1 ............. 13 2 1002 .....A..... 992 1 159 ............. 171 L I K I Q K L L F K V L Q V L K D Y S T Q N K * L N T V R K Query_1 1396 TTAATAAAGATACAAAAGTTATTGTTCAAGGTATTACAGGTTCTCAAGGATTATTCCACACAAAACAAATGATTGAATACGGTACGAAAA 1485 0 14 .......................................................................................... 103 2 991 ...-.......G....-.....-.........NN.N.-..................-.....N.-.....-....G.......T...... 909 1 172 .....................................................................................N.... 261 1 142 ........... 132 L S V V * R Q V K V A L T L K V Y Q Y L I L * K M Q * K Q Q Query_1 1486 TTGTCGGTGGTGTAACGCCAGGTAAAGGTGGCACTGACATTGAAGGTGTACCAGTATTTGATACTGTAGAAGATGCAGTGAAAGCAACAG 1575 0 104 .......................................................................................... 193 2 908 ................N.-...........A.........-....-....T.......N......-.N..................-... 824 1 262 .......N............................................................................N..... 351 A Q T L Q L Y T F H P L L R L M Q L W K Q L M Q K L I * * Query_1 1576 GCGCAAACGCTTCAGTTGTATACGTT-CCACCCGCTTTTGCGGCTGATGCAATTATGGAAGCAGTTGATGCAGAAA-TTGATTTAGTAGT 1663 0 194 ..........................-.................................................-............. 281 2 823 .N.......-................T...........-.....................................A.N........... 736 1 352 ..........................-.............................................N..N-...........N. 439 Y V L P K E F L Y * I W * T * R D I W L V N I H V Y L D Q Query_1 1664 ATGTATTACCGAA-GGAATTCCTGTATTAGATATGGTAAACGTAAAGAGATATATGGCTGGTAAACATACACGTTTACTTGGACCAAA-C 1751 0 282 .............-..........................................................................-. 369 2 735 .......N.....A..........................................................................A. 646 1 440 .............-T..........................T....N.........................................-. 527 T A Q V L L R L M N V K S V L C Q D T F I K K V M * V S Y L Query_1 1752 TGCCC-AGGTGTTATTACGCCTGATGAATGTAAAATCGGTATTATGCCAGGATACATTCATAAAAAAGGTCATGTAGGTATCGTATCTCG 1840 0 370 .....-.................................................................................... 458 0 525 ........... 535 2 645 .....C.................................................................................... 556 2 489 ........... 479 1 528 .....-............N..................N..... 569 A L V H * R M R L Y I S * H K K V L A N L L L * V S A E T Q Query_1 1841 CTCTGGTACATTAACGTATGAGGCTGTACATCAGTTAACACAAGAAGGTATTGGCCAATCTACTGCTGTAGGTATCGGCGGAGACCCAGT 1930 0 459 .......................................................................................... 548 0 441 ........... 451 2 555 .............................................................N............................ 466 2 573 ........... 563 L T V Q T L L M H * K H L M K M K K H M L * L * L V K S V V Query_1 1931 TAACGGTACAGACTTTATTGATGCATTAAAAGCATTTAATGAAGATGAAGAAACACATGCTGTAATTATGATTGGTGAAATCGGTGGTAC 2020 0 549 .......................................................................................... 638 2 465 .......................................................................................... 376 Q R K K R Q L N G * K L I * Q N L L * A S L V A K Q R L L A Query_1 2021 AGCGGAAGAAGAGGCAGCTGAATGGGTGAAAGCTAATATGACAAAACCTGTTGTAGGCTTCATTGGTGGCCAAACAGCGCCTGCTGGCAA 2110 0 639 .......................................................................................... 728 2 375 .......................................................................................... 286 N V W A M L V Q S F L A V K E L L Q K R L K R W K L V V L K Query_1 2111 ACGTATGGGCCATGCTGGTGCAATCATTTCTGGCGGTAAAGGAACTGCTGCAGAAAAGATTAAAACGATGGAAGCTTGTGGTATTAAAGT 2200 0 729 .......................................................................................... 818 2 285 .......................................................................................... 196 * R K L Q Q L W E K R * S L S * K K K V Y L R L V K T T N I Query_1 2201 AGCGGAAACTCCAGCAGTTATGGGAGAAACGCTAATCTCTGTCTTAAAAGAAAAAGGTCTATTTGAGACTTGTAAAAACTACTAACATTG 2290 0 819 .................................................................................. 900 2 195 .......................................................................................... 106 G S G * R I R I R A P S T S L R P H S S T T T T T T E I R Query_1 2291 GAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccacc-accacc-acTGA-GATCCGG 2376 2 105 ....................................................................T......A.....N....... 17 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 6387420 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 2,634 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP02187.105 (IDP02187.1-387.F..pMCSG73)
MNLHEYQAKDLLESYGLKVQKGIVAHNPNEAAQAFDQLGGKFAVVKAQVHAGGRGKAGGVKVVKSSQEAREVAESLIGKNLVTFQTDAEGQPVNSVGVFEDVYPVTRELYLGAVVDRSSRKVTFMASTEGGVDIEEVAHNSPEKILKVEVDPLVGLQPFQAREVAFKLGLEGKQINDFVKTMLGAYKAFIECDFALFEINPLAVRENGEIVCVDGKINLDSNALYRHPKLLALRDKSQENAKELKASEHELNYVALEGNIGCMVNGAGLAMATMDIIQLYGGKPANFLDVGGGATKERVIEAFKLILDDENVKAILINIFGGIVRCDMIAEAIIEAVKEVNVTVPVVVRLEGNNAEKGAKILADSGLKLIPADGLADAADKVVKSLG
ATGAACTTACATGAATATCAAGCTAAAGATCTTTTAGAAAGCTATGGTCTAAAAGTTCAAAAAGGTATCGTAGCTCATAACCCAAATGAAGCTGCTCAAGCATTTGACCAATTAGGTGGCAAATTTGCTGTGGTTAAAGCTCAGGTACATGCTGGTGGTCGTGGTAAAGCTGGTGGTGTAAAGGTTGTTAAATCATCTCAAGAAGCTCGTGAAGTAGCAGAAAGCTTAATCGGTAAAAATCTTGTTACTTTCCAAACTGATGCTGAAGGTCAGCCAGTTAATTCTGTAGGTGTATTTGAAGATGTATACCCTGTTACTCGTGAGTTATACCTTGGTGCGGTAGTTGATAGATCTAGCCGTAAAGTAACATTCATGGCTTCAACTGAAGGCGGTGTAGATATTGAAGAAGTTGCACACAACTCTCCTGAAAAAATCCTTAAAGTAGAAGTAGATCCTTTAGTTGGCTTACAACCTTTCCAAGCTCGTGAAGTAGCTTTCAAGCTTGGTCTAGAAGGCAAGCAAATCAATGATTTTGTTAAAACTATGCTAGGCGCTTACAAAGCATTCATCGAATGTGATTTTGCCCTATTTGAAATCAACCCTCTTGCAGTTAGAGAAAACGGAGAGATTGTCTGTGTTGATGGTAAAATCAACCTTGATTCAAATGCTCTTTATAGACACCCTAAACTTTTAGCTCTAAGAGACAAATCTCAAGAAAATGCTAAAGAACTTAAAGCTTCAGAGCATGAGCTAAACTATGTAGCTCTTGAAGGTAACATCGGTTGTATGGTAAACGGTGCTGGTCTAGCAATGGCTACTATGGATATTATCCAATTATACGGTGGTAAGCCTGCTAACTTCCTAGATGTAGGTGGTGGTGCTACTAAAGAGAGAGTAATCGAAGCTTTCAAACTAATCCTAGATGATGAAAATGTAAAAGCTATTTTAATTAACATCTTCGGTGGTATTGTTCGTTGTGATATGATCGCTGAAGCAATTATTGAAGCTGTTAAAGAAGTAAATGTAACTGTACCAGTAGTTGTTCGTCTAGAAGGTAACAATGCAGAAAAAGGTGCTAAAATATTAGCTGATTCAGGTTTAAAACTAATACCTGCTGATGGTTTAGCTGATGCTGCTGATAAAGTTGTGAAATCACTAGGT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,655 total letters Query= IDP02187.105 Length=1566 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2094 0.0 2_04B_B8_IDP02187.105_v73F.seq 1865 0.0 1_02B_B8_IDP02187.105_T7Term.seq 1813 0.0 H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 111 CCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGC 200 2 45 ..........A........G....------......................... 93 2 27 ...C........... 41 A M N L H E Y Q A K D L L E S Y G L K V Q K G I V A H N P N Query_1 201 CATGAACTTACATGAATATCAAGCTAAAGATCTTTTAGAAAGCTATGGTCTAAAAGTTCAAAAAGGTATCGTAGCTC-ATAACCCAAATG 289 0 1 ............................................................................-............ 88 2 94 .............................................................................-............ 182 1 1159 ...........C.........-.. 1137 E A A Q A F D Q L G G K F A V V K A Q V H A G G R G K A Query_1 290 AAGCTGCTCAAG-CATTTGACC-AATTAGGTGGCAAATTTGCTGTGGTTAAAGCT-CAGGTACATGCTGGTGGTCGT-GGT-AAAGCTGG 374 0 89 ............-.........-................................-.....................-...-........ 173 2 183 ............-.........-................................-.....................-...-........ 267 1 1136 .-.N........G.....NN..C.........C--.....N.N............T.....................N...N........ 1050 G G V K V V K S S Q E A R E V A E S L I G K N L V T F Q T D Query_1 375 -TGGTGTAAAGGTTGTTAAATCATCTCAAGAAGCTCGTGAAGTAGCAGAAAGCTTAATCGGTAAAAATCTTGTTACTTTCCAAACTGATG 463 0 174 -......................................................................................... 262 0 479 ................ 494 2 268 -......................................................................................... 356 2 573 ................ 588 1 1049 G..NNN..........-.......N....NNGCT...........................NT...............-........... 962 1 748 ................ 733 A E G Q P V N S V G V F E D V Y P V T R E L Y L G A V V D R Query_1 464 CTGAAGGTCAGCCAGTTAATTCTGTAGGTGTATTTGAAGATGTATACCCTGTTACTCGTGAGTTATACCTTGGTGCGGTAGTTGATAGAT 553 0 263 .......................................................................................... 352 2 357 .......................................................................................... 446 1 961 ..........................................N.....-..............-.......................... 874 S S R K V T F M A S T E G G V D I E E V A H N S P E K I L K Query_1 554 CTAGCCGTAAAGTAACATTCATGGCTTCAACTGAAGGCGGTGTAGATATTGAAGAAGTTGCACACAACTCTCCTGAAAAAATCCTTAAAG 643 0 353 .......................................................................................... 442 2 447 .......................................................................................... 536 1 873 ..........................-.............................................-................. 786 V E V D P L V G L Q P F Q A R E V A F K L G L E G K Q I N D Query_1 644 TAGAAGTAGATCCTTTAGTTGG-CTTACAACCTTTCCAAGCTCGTGAAGTAGCTTTCAAGCTTGGTCTAGAAGGCAAGCAAATCAATGAT 732 0 443 ......................-................................................................... 531 0 203 ................ 218 2 537 ......................-................................................................... 625 2 297 ................ 312 1 785 ..............N.......G................................................................... 696 1 1016 ............ 1005 1 1032 ........... 1042 F V K T M L G A Y K A F I E C D F A L F E I N P L A V R E N Query_1 733 TTTGTTAAAACTATGCTAGGCGCTTACAAAGCATTCATCGAATGTGATTTTGCCCTATTTGAAATCAACCCTCTTGCAGTTAGAGAAAAC 822 0 532 .......................................................................................... 621 2 626 .......................................................................................... 715 1 695 .......................................................................................... 606 G E I V C V D G K I N L D S N A L Y R H P K L L A L R D K S Query_1 823 GGAGAGATTGTCTGTGTTGATGGTAAAATCAACCTTGATTCAAATGCTCTTTATAGACACCCTAAACTTTTAGCTCTAAGAGACAAATCT 912 0 622 .......................................................................................... 711 2 716 .......................................................................................... 805 1 605 .......................................................................................... 516 Q E N A K E L K A S E H E L N Y V A L E G N I G C M V N G A Query_1 913 CAAGAAAATGCTAAAGAACTTAAAGCTTCAGAGCATGAGCTAAACTATGTAGCTCTTGAAGGTAACATCGGTTGTATGGTAAACGGTGCT 1002 0 712 .......................................................................................... 801 2 806 ......................................N.....................N............................. 895 1 515 .......................................................................................... 426 1 1092 ............ 1081 G L A M A T M D I I Q L Y G G K P A N F L D V G G G A T K E Query_1 1003 GGTCTAGCAATGGCTACTATGGATATTATCCAATTATACGGTGGTAAGCCTGCTAACTTCCTAGATGTAGGTGGTGGTGCTACTAAAGAG 1092 0 802 .......................................................................................... 891 2 896 .........................................G.........................N...................... 985 1 425 .......................................................................................... 336 R V I E A F K L I L D D E N V K A I L I N I F G G I V R C Query_1 1093 AGAGTAA-TCGAAGCTTTCAAACTAATCCTAGATGATGAAAATGTAAAAGCTATTTTAATTAACATCTTCGGTGGTATTGTTCGTTGTGA 1181 0 892 .......-.................................................................................. 980 2 986 .......T.......................N.......................................--..NNN.N.......... 1073 1 335 .......-.................................................................................. 247 D M I A E A I I E A V K E V N V T V P V V V R L E G N N Query_1 1182 TATG-ATCGC-TGAAGCAATTATTGAAGC--TGTTAAAGAAGTAAAT-GTAACTGTACCAGTAG-TTGTTCGTCTAGAAGGTAACAATGC 1265 0 981 ....-.....-..................--................-................-......................... 1064 2 1074 ....A.N...T..................CTNN........NN....G............N.NNT...N.......-N.NNAN....... 1162 1 246 ....-.....-..................--................-................-......................... 163 A E K G A K I L A D S G L K L I P A D G L A D A A D K V V K Query_1 1266 AGAAAAAGGTGCTAAAATATTAGCTGATTCAGGTTTAAAACTAATACCTGCTGATGGTTTAGCTGATGCTGCTGATAAAGTTGTGAAATC 1355 0 1065 .......................................................................................... 1154 2 1163 ..-..N...N............ 1183 1 162 .......................................................................................... 73 S L G * H W K W I T D P N S S S V D K L A A A L E H H H H H Query_1 1356 ACTAGGTTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccacca 1445 0 1155 ....... 1161 1 72 ...........................--..... 41 H * D P A A N K A R K Query_1 1446 cTGAGATCCGGCTGCTAAC-AAAGCCC-GAAAGG 1477 1 45 ..............A.......NN..... 17 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5590850 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 3,655 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP02313.102 (IDP02313.1-300.F..pMCSG68)
MSVLVNKDTKVIVQGITGSQGLFHTKQMIEYGTKIVGGVTPGKGGTDIEGVPVFDTVEDAVKATGANASVVYVPPAFAADAIMEAVDAEIDLVVCITEGIPVLDMVNVKRYMAGKHTRLLGPNCPGVITPDECKIGIMPGYIHKKGHVGIVSRSGTLTYEAVHQLTQEGIGQSTAVGIGGDPVNGTDFIDALKAFNEDEETHAVIMIGEIGGTAEEEAAEWVKANMTKPVVGFIGGQTAPAGKRMGHAGAIISGGKGTAAEKIKTMEACGIKVAETPAVMGETLISVLKEKGLFETCKNY
ATGAGCGTATTAGTTAATAAAGATACAAAAGTTATTGTTCAAGGTATTACAGGTTCTCAAGGATTATTCCACACAAAACAAATGATTGAATACGGTACGAAAATTGTCGGTGGTGTAACGCCAGGTAAAGGTGGCACTGACATTGAAGGTGTACCAGTATTTGATACTGTAGAAGATGCAGTGAAAGCAACAGGCGCAAACGCTTCAGTTGTATACGTTCCACCCGCTTTTGCGGCTGATGCAATTATGGAAGCAGTTGATGCAGAAATTGATTTAGTAGTATGTATTACCGAAGGAATTCCTGTATTAGATATGGTAAACGTAAAGAGATATATGGCTGGTAAACATACACGTTTACTTGGACCAAACTGCCCAGGTGTTATTACGCCTGATGAATGTAAAATCGGTATTATGCCAGGATACATTCATAAAAAAGGTCATGTAGGTATCGTATCTCGCTCTGGTACATTAACGTATGAGGCTGTACATCAGTTAACACAAGAAGGTATTGGCCAATCTACTGCTGTAGGTATCGGCGGAGACCCAGTTAACGGTACAGACTTTATTGATGCATTAAAAGCATTTAATGAAGATGAAGAAACACATGCTGTAATTATGATTGGTGAAATCGGTGGTACAGCGGAAGAAGAGGCAGCTGAATGGGTGAAAGCTAATATGACAAAACCTGTTGTAGGCTTCATTGGTGGCCAAACAGCGCCTGCTGGCAAACGTATGGGCCATGCTGGTGCAATCATTTCTGGCGGTAAAGGAACTGCTGCAGAAAAGATTAAAACGATGGAAGCTTGTGGTATTAAAGTAGCGGAAACTCCAGCAGTTATGGGAGAAACGCTAATCTCTGTCTTAAAAGAAAAAGGTCTATTTGAGACTTGTAAAAACTAC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,324 total letters Query= IDP02313.102 Length=1305 Score E Sequences producing significant alignments: (Bits) Value 1_03D_D3_IDP02313.102_T7S.seq 1925 0.0 2_09D_D3_IDP02313.102_T7term.seq 1824 0.0 0_wt 1624 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 58 TTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCAT-CATCATCATTCTTCTGGTGTAGATCTGTG 146 1 6 .....T...N..................................................-............................. 94 2 1121 .....T.......N..N...N.NN-.......... 1088 W S H P Q F E K G T E N L Y F Q S N A M S V L V N K D T K Query_1 147 GT-CTCATCCGCAGTTCGAAAAGGGTACCGAG-AACCTGTACTTCCAATCCAATGCCATG-AGCGTATTAGTTAATAAAGATACAAAAGT 233 1 95 ..-.............................-...........................-............................. 181 1 207 ...-........ 197 2 1087 ..TN...........CN---..N.N.......G............A.N--..........G.................-........-.. 1005 2 980 ...-........ 990 0 1 ...-............................. 32 0 58 ...-........ 48 V I V Q G I T G S Q G L F H T K Q M I E Y G T K I V G G V T Query_1 234 TATTGTTCAAGGTATTACAGGTTCTCAAGGATTATTCCACACAAAACAAATGATTGAATACGGTACGAAAATTGTCGGTGGTGTAACGCC 323 1 182 .......................................................................................... 271 1 133 ........... 123 2 1004 ....N.-................................................................................... 916 0 33 .......................................................................................... 122 P G K G G T D I E G V P V F D T V E D A V K A T G A N A S V Query_1 324 AGGTAAA-GGTGGCACTGACATTGAAGGTGTACCAGTATTTGATACTGTAGAAGATGCAGTGAAAGCAACAGGCGCAAACGCTT-CAGTT 411 1 272 .......-............................................................................-..... 359 2 915 .......A............................................................................T..... 826 0 123 .......-............................................................................-..... 210 V Y V P P A F A A D A I M E A V D A E I D L V V C I T E G Query_1 412 GTATACGTTCCACCCGCTTTTGCGGCTGATGCAATT-ATGGAAGCAGTTGATGCAGAAATTGATTTAGTAGTATGTATTACCGAAGGAAT 500 1 360 ....................................-.............................G....................... 448 2 825 ....................................T.............................G....................... 736 0 211 ....................................-..................................................... 299 I P V L D M V N V K R Y M A G K H T R L L G P N C P G V I T Query_1 501 TCCTGTATTAGATATGGTAAACGTAAAGAGATATATGGCTGGTAAACATACACGTTTACTTGGACCAAACTGCCCAGGTGTTATTACGCC 590 1 449 .......................................................................................... 538 2 735 .......................................................................................... 646 0 300 .......................................................................................... 389 P D E C K I G I M P G Y I H K K G H V G I V S R S G T L T Y Query_1 591 TGATGAATGTAAAATCGGTATTATGCCAGGATACATTCATAAAAAAGGTCATGTAGGTATCGTATCTCGCTCTGGTACATTAACGTATGA 680 1 539 .......................................................................................... 628 1 674 ........... 684 2 645 .......................................................................................... 556 2 510 ........... 500 0 390 .......................................................................................... 479 0 525 ........... 535 E A V H Q L T Q E G I G Q S T A V G I G G D P V N G T D F I Query_1 681 GGCTGTACATCAGTTAACACAAGAAGGTATTGGCCAATCTACTGCTGTAGGTATCGGCGGAGACCCAGTTAACGGTACAGACTTTATTGA 770 1 629 .......................................................................................... 718 1 590 ........... 600 2 555 .......................................................................................... 466 2 594 ........... 584 0 480 .......................................................................................... 569 0 441 ........... 451 D A L K A F N E D E E T H A V I M I G E I G G T A E E E A A Query_1 771 TGCATTAAAAGCATTTAATGAAGATGAAGAAACACATGCTGTAATTATGATTGGTGAAATCGGTGGTACAGCGGAAGAAGAGGCAGCTGA 860 1 719 .......................................................................................... 808 2 465 .......................................................................................... 376 0 570 .......................................................................................... 659 E W V K A N M T K P V V G F I G G Q T A P A G K R M G H A G Query_1 861 ATGGGTGAAAGCTAATATGACAAAACCTGTTGTAGGCTTCATTGGTGGCCAAACAGCGCCTGCTGGCAAACGTATGGGCCATGCTGGTGC 950 1 809 ........................N.........................-....................................... 897 2 375 .......................................................................................... 286 0 660 .......................................................................................... 749 A I I S G G K G T A A E K I K T M E A C G I K V A E T P A V Query_1 951 AATCATTTCTGGCGGTAAAGGAACTGCTGCAGAAAAGATTAAAACGATGGAAGCTTGTGGTATTAAAGTAGCGGAAACTCCAGCAGTTAT 1040 1 898 .T....................................-................................................... 986 2 285 .......................................................................................... 196 0 750 .......................................................................................... 839 M G E T L I S V L K E K G L F E T C K N Y * H W K W I T D Query_1 1041 GGGAGAAACGCTAA-TCTC-TGTCTTAAAAGAAAAAGGTCTA-TTTGAGACTTGTAAAAACTACTAACATTGG-AAGTGGATAACGGATC 1126 1 987 .....-........T....T......................T.....N.....N...........N......A........-....... 1074 2 195 ..............-....-......................-..............................-................ 110 0 840 ..............-....-......................-..................... 900 P N S S S V D K L A A A L E H H H H H H * D P A A N K A R Query_1 1127 CGAATTCGAGC-TCCGTCGACAAGCTTGCGGCCGCAC-TCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAAC-AAAGCCCGAA 1213 1 1075 ..-.N......T.........................T..N....-.....-..NA.T.....NN...N.N.......N-..--NN.... 1158 2 109 ...........-.........................-.........................................A.......... 22 K E A E L A A A T A E Q * L A * P L G A S Query_1 1214 AGGAAG-CTGAGTTGGCTGCTGCCAC-CGCTGAGCAATAACTAGCATAACCCCTT--GGGGCCTCT 1275 1 1159 N.N...C....NN....N...NA...T.NN..NAN......N.N.N......NA.NN....A.... 1224 2 21 . 21 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4222764 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 3,324 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP02692.103 (IDP02692.1-290.F..pMCSG73)
MSVLVDKNTKVLVQGFTGKNGTFHSEQAIAYGTNIVGGVTPGKGGTTHLDRPVFNTMAEAVAATGADASVIYVPAPFVKDSAIEAIDSGVKLVVIITEGVPTLDMLVVKEYLKDKDVRVIGPNCPGIITPGECKIGIMPGHIHMKGKVGIISRSGTLTYEAVAQTTKLGFGQSTCIGIGGDPIPGMNQIEALKLLENDPQTEAIILIGEIGGTAEEEAAEYIKHNVTKPVIGYIAGVTAPPGKRMGHAGAIISGGKGTAEEKFAAFEAAGIAYTRSPAEIGKKLKEVTGW
ATGAGCGTATTAGTTGATAAAAATACAAAAGTTTTAGTACAGGGTTTTACTGGTAAAAATGGTACTTTCCATTCAGAACAAGCTATTGCTTATGGTACAAATATTGTAGGTGGCGTAACTCCTGGTAAAGGTGGCACTACTCACTTAGATAGACCAGTGTTCAATACAATGGCTGAAGCTGTAGCAGCTACTGGTGCTGATGCGTCTGTAATCTATGTTCCAGCTCCATTTGTAAAAGACTCTGCAATTGAAGCGATCGATTCTGGTGTTAAGCTAGTAGTAATCATTACAGAAGGTGTCCCTACTCTAGATATGCTAGTAGTTAAAGAGTACTTAAAAGATAAAGATGTGAGAGTAATTGGTCCTAACTGTCCAGGGATCATCACTCCAGGTGAGTGTAAGATTGGTATTATGCCGGGTCATATTCATATGAAAGGTAAAGTTGGTATCATTTCTCGTTCTGGTACTTTAACTTACGAAGCAGTTGCTCAAACTACTAAACTAGGCTTTGGTCAATCTACTTGTATTGGTATAGGCGGAGACCCTATCCCAGGTATGAACCAAATCGAAGCTCTTAAACTTTTAGAGAATGACCCACAAACTGAAGCTATCATCTTAATCGGTGAAATTGGTGGTACAGCTGAAGAAGAAGCTGCTGAATATATCAAGCACAATGTAACTAAGCCTGTTATTGGATATATCGCTGGTGTTACAGCTCCTCCAGGAAAGCGTATGGGTCATGCTGGTGCTATTATCTCTGGTGGTAAAGGTACCGCTGAAGAAAAATTTGCCGCATTCGAAGCTGCTGGTATTGCTTATACTAGATCACCTGCTGAGATCGGCAAGAAGCTTAAAGAAGTTACTGGCTGG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,253 total letters Query= IDP02692.103 Length=1275 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1570 0.0 2_04A_A8_IDP02692.103_v73F.seq 156 3e-41 1_02A_A8_IDP02692.103_T7Term.seq 64.4 2e-13 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCAT-CCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAA 197 2 44 .........T..A........G....------...................... 91 2 27 ...C........... 41 2 28 .............. 41 1 935 ...NC.........T..A........G.N..------...................... 883 N A M S V L V D K N T K V L V Q G F T G K N G T F H S E Q A Query_1 198 TGCCATGAGCGTATTAGTTGATAAAAATACAAAAGTTTTAGTACAGGGTTTTACTGGTAAAAATGGTACTTTCCATTCAGAACAAGCTAT 287 0 1 ...................................................................................... 86 2 92 ........ 99 1 882 ....... 876 I A Y G T N I V G G V T P G K G G T T H L D R P V F N T M A Query_1 288 TGCTTATGGTACAAATATTGTAGGTGGCGTAACTCCTGGTAAAGGTGGCACTACTCACTTAGATAGACCAGTGTTCAATACAATGGCTGA 377 0 87 .......................................................................................... 176 E A V A A T G A D A S V I Y V P A P F V K D S A I E A I D S Query_1 378 AGCTGTAGCAGCTACTGGTGCTGATGCGTCTGTAATCTATGTTCCAGCTCCATTTGTAAAAGACTCTGCAATTGAAGCGATCGATTCTGG 467 0 177 .......................................................................................... 266 G V K L V V I I T E G V P T L D M L V V K E Y L K D K D V R Query_1 468 TGTTAAGCTAGTAGTAATCATTACAGAAGGTGTCCCTACTCTAGATATGCTAGTAGTTAAAGAGTACTTAAAAGATAAAGATGTGAGAGT 557 0 267 .......................................................................................... 356 V I G P N C P G I I T P G E C K I G I M P G H I H M K G K V Query_1 558 AATTGGTCCTAACTGTCCAGGGATCATCACTCCAGGTGAGTGTAAGATTGGTATTATGCCGGGTCATATTCATATGAAAGGTAAAGTTGG 647 0 357 .......................................................................................... 446 G I I S R S G T L T Y E A V A Q T T K L G F G Q S T C I G I Query_1 648 TATCATTTCTCGTTCTGGTACTTTAACTTACGAAGCAGTTGCTCAAACTACTAAACTAGGCTTTGGTCAATCTACTTGTATTGGTATAGG 737 0 447 .......................................................................................... 536 G G D P I P G M N Q I E A L K L L E N D P Q T E A I I L I G Query_1 738 CGGAGACCCTATCCCAGGTATGAACCAAATCGAAGCTCTTAAACTTTTAGAGAATGACCCACAAACTGAAGCTATCATCTTAATCGGTGA 827 0 537 .......................................................................................... 626 E I G G T A E E E A A E Y I K H N V T K P V I G Y I A G V T Query_1 828 AATTGGTGGTACAGCTGAAGAAGAAGCTGCTGAATATATCAAGCACAATGTAACTAAGCCTGTTATTGGATATATCGCTGGTGTTACAGC 917 0 627 .......................................................................................... 716 A P P G K R M G H A G A I I S G G K G T A E E K F A A F E A Query_1 918 TCCTCCAGGAAAGCGTATGGGTCATGCTGGTGCTATTATCTCTGGTGGTAAAGGTACCGCTGAAGAAAAATTTGCCGCATTCGAAGCTGC 1007 0 717 .......................................................................................... 806 A G I A Y T R S P A E I G K K L K E V T G W * H W K W I T D Query_1 1008 TGGTATTGCTTATACTAGATCACCTGCTGAGATCGGCAAGAAGCTTAAAGAAGTTACTGGCTGGTAACATTGGAAGTGG-ATAACGGATC 1096 0 807 ................................................................ 870 2 903 ...............-.....--... 925 1 67 ...............A.....--... 44 P N S S S V D K L A A A L E H H H H H H * D P A A N K A R K Query_1 1097 CGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGG 1186 2 923 ........................... 949 2 926 .. 927 1 46 .................N...N... 22 1 43 .. 42 E A E L A A A T A E Q * L A * P L G A S K R V L R G F L Query_1 1187 AAGCTGAGTTGGC-TGCTGCCACCGCTGAG-CAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTT-GAGGGGTTTTTTGC 1270 2 950 .............T........-.......C.............................CT...N......G.....A........ 1035 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4035095 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 3,253 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP90676.103 (IDP90676.1-387.F..pMCSG73)
MNIHEYQAKAIFVDNGIPTLKGKVAFSVDEAVANAKELGGSVWAVKAQIHAGGRGLGGGVKIAKNLDEVKDYASKILGMNLVTHQTGPEGKLVQKLYIESGANIVKEYYLAILFNRMAEQITIIASSEGGMDIEKVAKESPEKIAKVGIDPQIGFKMFHGLEVARVLGLDKDEGKKLISMIAKLYKLYMDKDMNMLEINPLIKTAEGDFYALDAKCSFDDSALYRHPEIAELRDTTEENPAEREAAEFGLSYVKLDGDVACMVNGAGLAMATMDIINYSGAKPANFLDVGGGASPETVAKAFEIILRDKNVKVIFINIFGGIVRCDRIANGILEATKNVEVNIPIVVRLDGTNAAEAKTILDNSNLKNIKAATNLKNGAELVKSLVG
ATGAACATACATGAGTATCAAGCAAAAGCAATTTTTGTAGACAATGGCATCCCTACCTTAAAGGGAAAAGTTGCATTTAGCGTAGATGAAGCTGTGGCTAATGCTAAAGAATTAGGCGGTAGTGTTTGGGCAGTTAAAGCTCAAATTCATGCAGGAGGCCGTGGTCTTGGTGGTGGTGTTAAAATCGCTAAGAATTTAGATGAGGTAAAAGATTATGCAAGTAAAATTTTAGGAATGAATTTAGTAACTCATCAAACAGGACCTGAAGGCAAACTTGTGCAAAAACTTTATATAGAAAGTGGTGCTAATATAGTAAAAGAGTATTATTTGGCTATTTTGTTTAATAGAATGGCAGAGCAAATTACCATTATCGCTTCAAGTGAAGGAGGGATGGATATAGAAAAAGTAGCCAAAGAAAGTCCTGAAAAAATTGCAAAAGTAGGTATTGATCCACAAATTGGATTTAAAATGTTTCATGGACTTGAAGTAGCGAGAGTTTTGGGACTTGATAAAGATGAAGGCAAAAAACTCATTTCTATGATAGCTAAACTTTATAAGCTTTACATGGATAAAGATATGAATATGCTTGAAATTAATCCTTTAATTAAAACTGCAGAAGGGGATTTTTATGCCCTTGATGCAAAATGCAGTTTTGATGATAGTGCGCTTTATCGTCATCCTGAAATTGCTGAACTTAGAGATACTACTGAAGAAAATCCTGCTGAAAGAGAAGCGGCTGAATTTGGCTTAAGCTATGTGAAATTAGATGGAGATGTAGCTTGTATGGTAAATGGTGCGGGTCTAGCAATGGCAACTATGGATATTATTAATTATAGTGGTGCAAAACCTGCAAATTTCTTAGATGTAGGTGGGGGAGCTTCTCCAGAAACTGTTGCAAAGGCTTTTGAAATTATTTTAAGAGATAAAAATGTAAAAGTTATATTTATCAATATCTTTGGTGGTATTGTTCGTTGTGATAGGATAGCAAATGGAATTTTAGAAGCTACTAAGAATGTTGAGGTAAATATTCCAATTGTAGTGCGTCTTGATGGCACTAACGCAGCAGAAGCAAAGACAATTTTAGATAATTCAAATCTTAAAAATATCAAAGCAGCAACCAATCTTAAAAACGGTGCAGAGCTTGTAAAAAGTTTAGTAGGA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,560 total letters Query= IDP90676.103 Length=1566 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2094 0.0 2_03G_G7_IDP90676.103_v73F.seq 1737 0.0 1_01G_G7_IDP90676.103_T7Term.seq 1673 0.0 F V * L * E G D I H M H H H H H H S S G V D L W S H P Q F Query_1 74 ATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCG 163 0 334 ........... 344 2 45 ...........A....... 63 2 27 .....C........... 43 2 429 ........... 439 1 907 ........... 897 E K G T E N L Y F Q S N A M N I H E Y Q A K A I F V D N G I Query_1 164 AAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGAACATACATGAGTATCAAGCAAAAGCAATTTTTGTAGACAATGGCATCC 253 0 1 .................................................... 52 2 64 .G....------.............................................................................. 147 P T L K G K V A F S V D E A V A N A K E L G G S V W A V Query_1 254 CTACCTTAAAGGGAAAAGTTGCATTTAGCGTAGATGAAGCTGTGGCTAATGCTAAAGA--ATTAG-GCGGTA-GTGTTT--GGGCAGTT- 336 0 53 ..........................................................--.....-......-......--........- 135 2 148 ..........................................................--.....-......-......--........- 230 1 1162 ....N......GAN....N......N.N....TG..N.....T 1120 K A Q I H A G G R G L G G G V K I A K N L D E V K D Y A Query_1 337 AAAGCT-CAAATTCATGCAGGAGGCCGTGGTCTTGGTGGTGGTG--TTAAAATCGCTAAGAATTTAGATGAGG-TAAAAGATTATGCAAG 422 0 136 ......-.....................................--...........................-................ 221 2 231 ......-.....................................--...........................-................ 316 1 1119 ......T.......N......G.....N...........N..NNGT.......N.N.........G.......N..........NT.C.. 1030 S K I L G M N L V T H Q T G P E G K L V Q K L Y I E S G Query_1 423 TAAAATTTTAGG-AATGAATTTA--GTAACTCATCAAAC-AGGACCTGAA--GGCAAACTTGTGCAAAAA-CTTTATATAGAAAGTGGTG 505 0 222 ............-..........--..............-..........--..................-................... 304 2 317 ............-..........--..............-..........--..................-................... 399 1 1029 ....N.......G.........TAG.N.C......N...C........N.NA.C...NN...........A................... 940 A N I V K E Y Y L A I L F N R M A E Q I T I I A S S E Query_1 506 CTAA-TATAGTAAAA--GAGTATTATTTGGCTATTTTGTTTAATAGAATGGCAG-AGCAAATTACCATTAT-CGCTT-CAAGTGAA--GG 587 0 305 ....-..........--.....................................-................-.....-........--.. 386 2 400 ....-..........--.....................................-................-.....-........--.. 481 1 939 ....A..........AGN....................................GN...............T.....T......G.AG.. 850 G G M D I E K V A K E S P E K I A K V G I D P Q I G F K M Query_1 588 AGGGATGGATATAGAAAAA-GTAGCCAAA-GAAAGTCCTGAAAAAA-TTGCAAAAGTAGGTATTGATCCACAAA-TTGGATTT-AAAATG 672 0 387 ...................-.........-................-...........................-........-...... 471 2 482 ...................-.........-................-...........................-........-...... 566 1 849 ...................A.........A................A...........................A........T...... 760 F H G L E V A R V L G L D K D E G K K L I S M I A K L Y K Query_1 673 TTT-CATGGACTTGAAGTAGCGAGAGTTTTGGGACTTGATAAAGATGAAGGCAAAAAACTCATTTCTATGATAGCTAAACTTTATAAGCT 761 0 472 ...-...................................................................................... 560 2 567 ...-...................................................................................... 655 1 759 ...T...................................................................................... 670 L Y M D K D M N M L E I N P L I K T A E G D F Y A L D A K C Query_1 762 TTACATGGATAAAGATATGAATATGCTTGAAATTAATCCTTTAATTAAAACTGCAGAAGGGGATTTTTATGCCCTTGATGCAAAATGCAG 851 0 561 .......................................................................................... 650 2 656 .......................................................................................... 745 1 669 .......................................................................................... 580 S F D D S A L Y R H P E I A E L R D T T E E N P A E R E A A Query_1 852 TTTTGATGATAGTGCGCTTTATCGTCATCCTGAAATTGCTGAACTTAGAGATACTACTGAAGAAAATCCTGCTGAAAGAGAAGCGGCTGA 941 0 651 .......................................................................................... 740 2 746 .......................................................................................... 835 1 579 .......................................................................................... 490 E F G L S Y V K L D G D V A C M V N G A G L A M A T M D I I Query_1 942 ATTTGG-CTTAAGCTATGTGAAATTAGATGGAGATGTAGCTTGTATGGTAAATGGTGCGGGTCTAGCAATGGCAACTATGGATATTATTA 1030 0 741 ......-................................................................................... 829 2 836 ......N....................................C.............................C................ 925 1 489 ......-................................................................................... 401 N Y S G A K P A N F L D V G G G A S P E T V A K A F E I I L Query_1 1031 ATTATAGTGGTGCAAAACCTGCAAATTTCTTAGATGTAGGTGGGGGAGCTTCTCCAGAAACTGTTGCAAAGGCTTTTGAAATTATTTTAA 1120 0 830 .......................................................................................... 919 2 926 ......................-................--T...............-............-.......-.....-.N... 1008 1 400 .......................................................................................... 311 R D K N V K V I F I N I F G G I V R C D R I A N G I L E A T Query_1 1121 GAGATAAAAATGTAAAAGTTATATTTATCAATATCTTTGGTGGTATTGTTCGTTGTGATAGGATAGCAAATGGAATTTTAGAAGCTACTA 1210 0 920 .......................................................................................... 1009 2 1009 .....-.......-.....N.........-.NN............-..G..CNNNNN.........-NNN....--N............. 1091 1 310 .......................................................................................... 221 K N V E V N I P I V V R L D G T N A A E A K T I L D N S N L Query_1 1211 AGAATGTTGAGGTAAATATTCCAATTGTAGTGCGTCTT-GATGGCACTAACGCAGCAGAAGCAAAGACAATTTTAGATAATTCAAATCTT 1299 0 1010 ......................................-................................................... 1098 2 1092 GC........-.N..CNT.........N..-N......G......C.N..... 1142 1 220 ......................................-................................................... 132 K N I K A A T N L K N G A E L V K S L V G * H W K W I T D P Query_1 1300 AAAAATATCAAAGCAGCAACCAATCTTAAAAACGGTGCAGAGCTTGTAAAAAGTTTAGTAGGATAACATTGGAAGTGGATAACGGATCCG 1389 0 1099 ............................................................... 1161 1 131 ................................................................................... 49 N S S S V D K L A A A L E H H H H H H * D P A A N K A Query_1 1390 AATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCC 1471 1 45 ................... 27 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5443600 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 3,560 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP90715.103 (IDP90715.1-289.F..pMCSG73)
MSILVNKNTKVIVQGFTGKEATFHAEQCMAYGTNIVGGITPHKGGQTHLGKPVFDTVADAVKATKADVSLIFVPAFAVGDSVIEAADAGIKLAVVITEHTPVKDMMFAKQYANKKGMKIIGPNCPGIITSEECKLGIMPGFIFKKGCVGLISKSGTLTYEAANQVVQGGYGISTAVGIGGDPIIGLAYKELLSEFQKDDETKAIVMIGEIGGSLEVEAAKFIKENISKPVVAFIAGATAPKGKRMGHAGAIVGSADESAAAKKEALKSYGIHVVDSPALIGEEIQKILG
ATGAGTATATTGGTTAATAAAAATACAAAAGTAATAGTTCAAGGTTTTACAGGTAAAGAAGCAACTTTTCATGCCGAGCAATGCATGGCTTATGGTACAAATATAGTCGGAGGCATCACTCCTCATAAAGGTGGACAAACTCATTTAGGTAAACCAGTTTTTGATACCGTTGCAGATGCGGTTAAAGCAACAAAGGCTGACGTGAGCTTGATCTTTGTACCTGCTTTTGCTGTAGGTGATAGCGTGATTGAAGCAGCTGATGCGGGTATTAAGCTTGCAGTTGTAATCACCGAGCATACGCCTGTAAAGGATATGATGTTTGCTAAGCAATATGCTAATAAAAAGGGTATGAAAATCATTGGACCAAATTGTCCAGGTATTATCACTTCTGAAGAATGTAAGTTAGGTATTATGCCAGGTTTTATTTTTAAAAAAGGTTGCGTGGGGCTTATTTCAAAAAGTGGAACTTTAACTTATGAAGCTGCTAATCAGGTAGTTCAAGGTGGTTATGGAATTTCTACTGCAGTAGGAATTGGTGGTGATCCTATCATAGGTCTTGCTTATAAAGAACTTTTAAGTGAATTCCAAAAAGATGATGAAACTAAAGCTATTGTAATGATAGGTGAAATTGGTGGTAGTTTGGAAGTGGAAGCGGCTAAATTTATTAAAGAAAATATTAGCAAACCTGTGGTAGCATTCATTGCAGGTGCAACTGCTCCTAAAGGCAAAAGAATGGGGCATGCAGGTGCCATAGTAGGAAGTGCTGATGAAAGTGCTGCAGCTAAAAAAGAAGCTTTAAAAAGCTATGGAATTCATGTGGTGGATTCTCCGGCTTTAATTGGTGAAGAAATTCAAAAAATATTAGGT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,378 total letters Query= IDP90715.103 Length=1272 Score E Sequences producing significant alignments: (Bits) Value 2_03H_H7_IDP90715.103_v73F.seq 1669 0.0 1_01H_H7_IDP90715.103_T7Term.seq 1649 0.0 0_wt 1564 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACC-TGTACTTCCAATCCAA 197 2 46 ...........A........G....------......-................ 92 2 28 ....NC........... 44 1 986 ...........A........G....------......C................ 939 1 1004 .....C........... 988 N A M S I L V N K N T K V I V Q G F T G K E A T F H A E Q C Query_1 198 TGCCATGAGTATATTGGTTAATAAAAATACAAAAGTAATAGTTCAAGGTTTTACAGGTAAA-GAAGCAACTTTTCATGCCGAGCAATGCA 286 2 93 .............................................................-............................ 181 2 590 ........... 600 1 938 .............................................................A............................ 849 1 439 ........... 429 0 1 .........................................................-............................ 85 0 494 ........... 504 M A Y G T N I V G G I T P H K G G Q T H L G K P V F D T V A Query_1 287 TGGCTTATGGTACAAA-TATAGTCGGAGGCATCACTCCTCATAAAGGTGGACAAACTCATTTAGGTAAACCAGTTTTTGATACCGTTGCA 375 2 182 ................-......................................................................... 270 1 848 ................A......................................................................... 759 0 86 ................-......................................................................... 174 D A V K A T K A D V S L I F V P A F A V G D S V I E A A D A Query_1 376 GATGCGGTTAAAGCAACAAAGGCTGACGTGAGCTTGATCTTTGTACCTGCTTTTGCTGTAGGTGATAGCGTGATTGAAGCAGCTGATGCG 465 2 271 .......................................................................................... 360 1 758 .......................................................................................... 669 0 175 .......................................................................................... 264 G I K L A V V I T E H T P V K D M M F A K Q Y A N K K G M K Query_1 466 GGTATTAAGCTTGCAGTTGTAATCACCGAGCATACGCCTGTAAAGGATATGATGTTTGCTAAGCAATATGCTAATAAAAAGGGTATGAAA 555 2 361 .......................................................................................... 450 1 668 .......................................................................................... 579 0 265 .......................................................................................... 354 I I G P N C P G I I T S E E C K L G I M P G F I F K K G C V Query_1 556 ATCATTGGACCAAATTGTCCAGGTATTATCACTTCTGAAGAATGTAAGTTAGGTATTATGCCAGGTTTTATTTTTAAAAAAGGTTGCGTG 645 2 451 .......................................................................................... 540 1 578 .......................................................................................... 489 0 355 .......................................................................................... 444 G L I S K S G T L T Y E A A N Q V V Q G G Y G I S T A V G I Query_1 646 GGGCTTATTTCAAAAAGTGGAACTTTAACTTATGAAGCTGCTAATCAGGTAGTTCAAGGTGGTTATGGAATTTCTACTGCAGTAGGAATT 735 2 541 .......................................................................................... 630 2 630 ....C............A.... 609 2 131 ........... 141 1 488 .......................................................................................... 399 1 399 ....C............A.... 420 1 900 ........... 890 0 445 .......................................................................................... 534 0 534 ....C............A.... 513 0 35 ........... 45 G G D P I I G L A Y K E L L S E F Q K D D E T K A I V M I G Query_1 736 GGTGGTGATCCTATCATAGGTCTTGCTTATAAAGAACTTTTAAGTGAATTCCAAAAAGATGATGAAACTAAAGCTATTGTAATGATAGGT 825 2 631 .......................................................................................... 720 1 398 .......................................................................................... 309 0 535 .......................................................................................... 624 E I G G S L E V E A A K F I K E N I S K P V V A F I A G A T Query_1 826 GAAATTGGTGGTAGTTTGGAAGTGGAAGCGGCTAAATTTATTAAAGAAAATATTAGCAAACCTGTGGTAGCATTCATTGCAGGTGCAACT 915 2 721 .......................................................................................... 810 2 969 ........... 979 1 308 .......................................................................................... 219 1 60 ........... 50 0 625 .......................................................................................... 714 A P K G K R M G H A G A I V G S A D E S A A A K K E A L K S Query_1 916 GCTCCTAAAGGCAAAAGAATGGGGCATGCAGGTGCCATAGTAGGAAGTGCTGATGAAAGTGCTGCAGCTAAAAAAGAAGCTTTAAAAAGC 1005 2 811 .......................................................................................... 900 1 218 .......................................................................................... 129 0 715 .......................................................................................... 804 Y G I H V V D S P A L I G E E I Q K I L G * H W K W I T D P Query_1 1006 TATGGAATTCATGTGGTGGATTCTCCGGCTTTAATTGGTGAAGAAATTCAAAAAATATTAGGTTAACATTGGAAGTGGATAACGGATCCG 1095 2 901 ...................................................................................--..... 988 2 736 ........... 746 1 128 ...................................................................................--..... 41 1 293 ........... 283 0 805 ............................................................... 867 0 640 ........... 650 N S S S V D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 1096 AATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAA 1185 2 984 ................-.......G.--. 1009 1 45 .........................A... 17 A E L A A A T A E Q * L A * P L G A S K R V L R G F L L Query_1 1186 GCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCAT-AACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAA 1271 2 1010 .........N-......NN.-.................N.A..........N.NN.......-..N.....N..--........... 1091 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4182480 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 3,378 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP91402.102 (IDP91402.24-250.P(250)..pMCSG68)
EEVVKVGMSGRYFPFTFVKQDQLQGFEVDMWDEIGKRNGYKVEYVTANFSGLFGLLETGRIDTISNQITMTDARKAKYLFADPYVVDGAQITVRKGNDSIKGVADLAGKTVAVNLGSNFEQLLRDYDKDGKINIKTYDTGIEHDVALGRADAFVMDRLSALELIKKTGLPLQLAGEPFETIANAWPFVNNERGVKLQGEVNKALAEMRADGTVEQISIKWFGADITK
GAAGAAGTGGTTAAAGTGGGTATGTCTGGCCGCTACTTCCCATTCACCTTTGTAAAGCAAGACCAACTGCAAGGTTTTGAAGTGGATATGTGGGATGAGATTGGTAAGCGTAATGGCTACAAAGTCGAATACGTAACCGCAAACTTCTCAGGTCTGTTTGGCTTACTTGAAACGGGTCGCATCGATACCATTTCCAACCAAATCACCATGACGGATGCGCGCAAAGCCAAATACCTTTTTGCTGATCCTTATGTGGTAGACGGCGCTCAGATCACCGTACGCAAGGGCAATGACAGCATCAAAGGCGTTGCAGACCTTGCAGGTAAAACCGTTGCGGTCAACCTAGGCTCAAACTTTGAGCAGCTATTACGCGATTACGATAAAGACGGCAAAATCAATATCAAGACTTACGATACCGGTATTGAACATGATGTTGCGTTAGGACGTGCCGATGCATTCGTGATGGATCGCCTGTCAGCACTAGAACTGATCAAGAAAACCGGTTTGCCACTACAATTGGCAGGTGAACCGTTTGAAACCATCGCTAACGCATGGCCATTTGTGAACAACGAACGTGGCGTGAAGCTGCAAGGTGAAGTCAACAAAGCCCTTGCTGAGATGCGCGCAGACGGTACGGTTGAGCAAATCTCAATCAAATGGTTTGGTGCAGACATCACCAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,127 total letters Query= IDP91402.102 Length=1086 Score E Sequences producing significant alignments: (Bits) Value 1_03C_C3_IDP91402.102_T7S.seq 1817 0.0 2_09C_C3_IDP91402.102_T7term.seq 1764 0.0 0_wt 1229 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAA 90 1 5 .........N...-................... 36 2 1013 ......N..-...........................-.................................................... 926 2 948 ...G...........T.T.C.C.C......... 980 E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E N Query_1 91 GAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAAC 180 1 37 .......................................................................................... 126 2 925 ..-....................................................................................... 837 L Y F Q S N A E E V V K V G M S G R Y F P F T F V K Q D Q L Query_1 181 CTGTACTTCCAATCCAATGCCGAAGAAGTGGTTAAAGTGGGTATGTCTGGCCGCTACTTCCCATTCACCTTTGTAAAGCAAGACCAACTG 270 1 127 .......................................................................................... 216 2 836 .......................................................................................... 747 0 1 ..................................................................... 69 Q G F E V D M W D E I G K R N G Y K V E Y V T A N F S G L F Query_1 271 CAAGGTTTTGAAGTGGATATGTGGGATGAGATTGGTAAGCGTAATGGCTACAAAGTCGAATACGTAACCGCAAACTTCTCAGGTCTGTTT 360 1 217 .......................................................................................... 306 2 746 .......................................................................................... 657 0 70 .......................................................................................... 159 G L L E T G R I D T I S N Q I T M T D A R K A K Y L F A D P Query_1 361 GGCTTACTTGAAACGGGTCGCATCGATACCATTTCCAACCAAATCACCATGACGGATGCGCGCAAAGCCAAATACCTTTTTGCTGATCCT 450 1 307 .......................................................................................... 396 2 656 .......................................................................................... 567 0 160 .......................................................................................... 249 Y V V D G A Q I T V R K G N D S I K G V A D L A G K T V A V Query_1 451 TATGTGGTAGACGGCGCTCAGATCACCGTACGCAAGGGCAATGACAGCATCAAAGGCGTTGCAGACCTTGCAGGTAAAACCGTTGCGGTC 540 1 397 .......................................................................................... 486 2 566 .......................................................................................... 477 0 250 .......................................................................................... 339 N L G S N F E Q L L R D Y D K D G K I N I K T Y D T G I E H Query_1 541 AACCTAGGCTCAAACTTTGAGCAGCTATTACGCGATTACGATAAAGACGGCAAAATCAATATCAAGACTTACGATACCGGTATTGAACAT 630 1 487 .......................................................................................... 576 2 476 .......................................................................................... 387 0 340 .......................................................................................... 429 D V A L G R A D A F V M D R L S A L E L I K K T G L P L Q L Query_1 631 GATGTTGCGTTAGGACGTGCCGATGCATTCGTGATGGATCGCCTGTCAGCACTAGAACTGATCAAGAAAACCGGTTTGCCACTACAATTG 720 1 577 .......................................................................................... 666 2 386 .......................................................................................... 297 0 430 .......................................................................................... 519 A G E P F E T I A N A W P F V N N E R G V K L Q G E V N K A Query_1 721 GCAGGTGAACCGTTTGAAACCATCGCTAACGCATGGCCATTTGTGAACAACGAACGTGGCGTGAAGCTGCAAGGTGAAGTCAACAAAGCC 810 1 667 .......................................................................................... 756 2 296 .......................................................................................... 207 0 520 .......................................................................................... 609 L A E M R A D G T V E Q I S I K W F G A D I T K * H W K W I Query_1 811 CTTGCTGAGATGCGCGCAGACGGTACGGTTGAGCAAATCTCAATCAAATGGTTTGGTGCAGACATCACCAAATAACATTGGAAGTGGATA 900 1 757 .......................................................................................... 846 2 206 .......................................................................................... 117 0 610 ........................................................................ 681 T D P N S S S V D K L A A A L E H H H H H H * D P A A N K Query_1 901 ACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAG-C 989 1 847 ........................................................................................-. 935 2 116 ........................................................................................C. 27 A R K E A E L A A A T A E Q * L A * P L G A S K R V L R G F Query_1 990 CCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTT 1079 1 936 ........................................................N............--C........-......... 1022 2 26 ........ 19 L L K Query_1 1080 GCTGAAA 1086 1 1023 ....... 1029 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3294530 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 3,127 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95098.103 (IDP95098.1-471.F..pMCSG73)
MLLPVIMAGGTGSRLWPMSRELYPKQFLRLFGQNSMLQETITRLSGLEIHEPMVICNEEHRFLVAEQLRQLNKLSNNIILEPVGRNTAPAIALAALQATRHGDDPLMLVLAADHIINNQPVFHDAIRVAEQYADEGHLVTFGIVPNAPETGYGYIQRGVALTDSAHTPYQVARFVEKPDRERAEAYLASGEYYWNSGMFMFRAKKYLSELAKFRPDILEACQAAVNAADNGSDFISIPHDIFCECPDESVDYAVMEKTADAVVVGLDADWSDVGSWSALWEVSPKDGQGNVLSGDAWVHNSENCYINSDEKLVAAIGVENLVIISTKDAVLVMNRERSQDVKKAVEFLKQNQRSEYKRHREIYRPWGRCDVVVQTPRFNVNRITVKPGGAFSMQMHHHRAEHWVILAGTGQVTVNGKQFLLTENQSTFIPIGAEHSLENPGRIPLEVLEIQSGSYLGEDDIIRIKDQYGRC
ATGTTGCTTCCTGTGATAATGGCTGGTGGTACCGGCAGTCGTCTCTGGCCGATGTCTCGCGAGCTTTATCCGAAACAGTTCCTCCGCCTGTTCGGGCAGAACTCCATGCTACAGGAAACCATCACCCGACTCTCGGGCCTTGAAATCCATGAACCGATGGTCATCTGTAACGAAGAGCACCGCTTCCTGGTGGCCGAACAGCTGCGCCAGCTCAACAAGCTGTCCAACAACATTATTCTTGAGCCGGTCGGGCGCAACACCGCCCCGGCCATCGCCCTGGCGGCCCTCCAGGCCACCCGCCACGGCGACGACCCGCTGATGCTGGTCCTCGCCGCCGACCATATCATCAATAACCAGCCGGTCTTCCACGACGCCATCCGCGTCGCCGAGCAGTATGCCGATGAAGGCCATCTGGTCACCTTCGGTATCGTGCCGAACGCCCCGGAAACCGGCTACGGCTACATCCAGCGCGGCGTGGCCCTCACCGACAGCGCCCATACTCCGTACCAGGTGGCCCGCTTCGTGGAGAAGCCGGACCGCGAGCGCGCCGAGGCTTACCTCGCCTCCGGGGAGTACTACTGGAACAGCGGCATGTTTATGTTCCGCGCCAAAAAATACCTTTCCGAGCTGGCCAAATTCCGCCCGGATATCCTCGAAGCCTGCCAGGCCGCGGTCAATGCCGCCGATAACGGCAGCGACTTCATCAGCATCCCGCATGACATTTTCTGCGAGTGTCCGGACGAGTCTGTGGACTACGCGGTGATGGAGAAAACCGCCGACGCGGTGGTGGTCGGTCTTGATGCCGACTGGAGCGACGTCGGCTCCTGGTCCGCCCTGTGGGAGGTCAGCCCGAAAGACGGGCAGGGTAACGTCCTCAGCGGCGACGCGTGGGTGCACAACAGCGAAAACTGCTACATCAACAGCGACGAGAAGCTGGTGGCGGCCATCGGCGTGGAGAATCTGGTGATTATCAGCACCAAGGACGCCGTGCTGGTGATGAACCGCGAGCGTTCCCAGGACGTGAAGAAGGCGGTCGAGTTTCTCAAGCAGAACCAGCGCAGCGAGTACAAGCGCCACCGCGAGATTTACCGTCCCTGGGGCCGCTGCGACGTGGTGGTCCAGACCCCGCGCTTCAACGTTAACCGCATTACGGTGAAACCGGGCGGCGCCTTCTCGATGCAGATGCATCACCACCGCGCCGAGCACTGGGTCATTCTCGCCGGCACCGGCCAGGTGACCGTCAACGGCAAGCAGTTCCTGCTGACCGAGAACCAGTCCACCTTTATTCCGATTGGCGCCGAGCACAGCCTGGAAAACCCGGGCCGCATTCCGCTGGAAGTGCTGGAGATCCAGTCGGGGTCGTACCTCGGCGAGGACGACATTATTCGTATTAAAGACCAGTATGGTCGTTGC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,820 total letters Query= IDP95098.103 Length=1818 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2549 0.0 2_10A_A4_IDP95098.103_T7_Term.seq 1902 0.0 1_04A_A4_IDP95098.103_v73F.seq 1754 0.0 S H P Q F E K G T E N L Y F Q S N A M L L P V I M A G G T Query_1 146 GGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGTTGCTTCCTGTGATAATGGCTGGTGGTACCG 235 0 1 .................................. 34 1 35 ..........A........G....------............................................................ 118 G S R L W P M S R E L Y P K Q F L R L F G Q N S M L Q E T I Query_1 236 GCAGTCGTCTCTGGCCGATGTCTCGCGAGCTTTATCCGAAACAGTTCCTCCGCCTGTTCGGGCAGAACTCCATGCTACAGGAAACCATCA 325 0 35 .......................................................................................... 124 1 119 .......................................................................................... 208 T R L S G L E I H E P M V I C N E E H R F L V A E Q L R Q L Query_1 326 CCCGACTCTCGGGCCTTGAAATCCATGAACCGATGGTCATCTGTAACGAAGAGCACCGCTTCCTGGTGGCCGAACAGCTGCGCCAGCTCA 415 0 125 .......................................................................................... 214 1 209 .......................................................................................... 298 N K L S N N I I L E P V G R N T A P A I A L A A L Q A T R H Query_1 416 ACAAGCTGTCCAACAACATTATTCTTGAGCCGGTCGGGCGCAACACCGCCCCGGCCATCGCCCTGGCGGCCCTCCAGGCCACCCGCCACG 505 0 215 .......................................................................................... 304 2 1188 ... 1186 1 299 .......................................................................................... 388 G D D P L M L V L A A D H I I N N Q P V F H D A I R V A Query_1 506 GCGACGACCCGCTGATG-CTGGT-CCTCGCCG-CCGACCATATCATCAATAACCAGCCGGTCTTCCACGACGCCATCCG-CGTCGCC-GA 590 0 305 .................-.....-........-..............................................-.......-.. 389 2 1185 .N..NN...NNT.....N.....T..NNN...N..NNN.............N...N...N...NN..........NNN.GN......C.. 1096 1 389 .................-.....-........-..............................................-.......-.. 473 E Q Y A D E G H L V T F G I V P N A P E T G Y G Y I Q R G Query_1 591 GCAGTATGCCGATGAAGGCCATCTGGTCACCTT-CGGTATCG-TGCCGAACGCCCCGGAAACCGGCTACGG-CTACATCCAGCGCGGCGT 677 0 390 .................................-........-............................-.................. 476 2 1095 .................................T........G........N..........N........G.................. 1006 1 474 .................................-........-............................-.................. 560 V A L T D S A H T P Y Q V A R F V E K P D R E R A E A Y L A Query_1 678 GGCCCTCACCGACAGCGCCCATACTCCGTACCAGGTGGCCCGCTTCGTGGAGAAGCCGGACCGCGAGCGCGCCGAGGCTTACCTCGCCTC 767 0 477 .......................................................................................... 566 2 1005 .......................................................................................... 916 1 561 .......................................................................................... 650 S G E Y Y W N S G M F M F R A K K Y L S E L A K F R P D I L Query_1 768 CGGGGAGTACTACTGGAACAGCGGCATGTTTATGTTCCGCGCC-AAAAAATACC-TTTCCGAGCTGGCCAAATTCCGCCCGGATATCCTC 855 0 567 ...........................................-..........-................................... 654 2 915 ...........................................C..........-................................... 827 1 651 ...........................................-..........T................................... 739 E A C Q A A V N A A D N G S D F I S I P H D I F C E C P D E Query_1 856 GAAGCCTGCCAGGCCGCGGTCAATGCCGCCGATAACGGCAGCGACTTCATCAGCATCCCGCATGACATTTTCTGCGAGTGTCCGGACGAG 945 0 655 .......................................................................................... 744 2 826 .......................................................................................... 737 1 740 .......................................................................................... 829 S V D Y A V M E K T A D A V V V G L D A D W S D V G S W S Query_1 946 TCTGTGGACTACGCGGTGATGGAGAAAACCGCCGACGCGGTGGTGGTCGGTCTTGATGCCGACTGGAGCGACGTCGGC-TCCTGGTCCGC 1034 0 745 ..............................................................................-........... 833 2 736 ..............................................................................-........... 648 1 830 ..............................................................................T........... 919 A L W E V S P K D G Q G N V L S G D A W V H N S E N C Y I N Query_1 1035 CCTGTGGGAGGTCAGCCCGAAAGACGGGCAGGGTAACGTCCTCAGCGGCGACGCGT-GGGTGCACAACAGCGAAAACTGCTACATCAACA 1123 0 834 ........................................................-................................. 922 2 647 ........................................................-................................. 559 1 920 ........................................................G................................. 1009 S D E K L V A A I G V E N L V I I S T K D A V L V M N R Query_1 1124 GCGACG-AGAA-GCT--GGTGGC-GGCCATCGGCGTGGAGAATCTGGTG-ATTATCAGCACCAAGGA-CGCCGTGCTGGTGATGAACCGC 1206 0 923 ......-....-...--......-.........................-.................-...................... 1005 2 558 ......-....-...--......-.........................-.................-...................... 476 1 1010 N.....A....NN..TG......G.........NN.-NNN.........A......NN..NNNNNNNC.....N.....NN....NGG.. 1098 E R S Q D V K K A V E F L K Q N Q R S E Y K R H R E I Y Query_1 1207 G--AGCGTTCCCAGGA-CGTGAAGAAGGCGGTCG-AGTTT-CTCAAGCAGAA-CCAGCGCAGCGAGTACAAGCGCCACCGCGAGATTTAC 1290 0 1006 .--.............-.................-.....-...........-..................................... 1089 2 475 .--.............-.................-.....-...........-..................................... 392 1 1099 .NANNNT.........C....-......NC...NA.....C......GC...C........N.. 1161 R P W G R C D V V V Q T P R F N V N R I T V K P G G A F S M Query_1 1291 CGTCCCTGGGGCCGCTGCGACGTGGTGGTCCAGACCCCGCGCTTCAACGTTAACCGCATTACGGTGAAACCGGGCGGCGCCTTCTCGATG 1380 0 1090 .......................................................................................... 1179 2 391 .......................................................................................... 302 Q M H H H R A E H W V I L A G T G Q V T V N G K Q F L L T E Query_1 1381 CAGATGCATCACCACCGCGCCGAGCACTGGGTCATTCTCGCCGGCACCGGCCAGGTGACCGTCAACGGCAAGCAGTTCCTGCTGACCGAG 1470 0 1180 .......................................................................................... 1269 0 1295 ........... 1305 2 301 .......................................................................................... 212 2 186 ........... 176 N Q S T F I P I G A E H S L E N P G R I P L E V L E I Q S G Query_1 1471 AACCAGTCCACCTTTATTCCGATTGGCGCCGAGCACAGCCTGGAAAACCCGGGCCGCATTCCGCTGGAAGTGCTGGAGATCCAGTCGGGG 1560 0 1270 .......................................................................................... 1359 0 1196 ........... 1206 2 211 .......................................................................................... 122 2 285 ........... 275 S Y L G E D D I I R I K D Q Y G R C * H W K W I T D P N S S Query_1 1561 TCGTACCTCGGCGAGGACGACATTATTCGTATTAAAGACCAGTATGGTCGTTGCTAACATTGGAAGTGGATAACGGATCCGAATTCGAGC 1650 0 1360 ...................................................... 1413 2 121 ..........................................................................--..... 43 S V D K L A A A L E H H H H H H * D P A A N K A R Query_1 1651 TCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAAC-AAAGCCCGAAA 1727 2 47 ..............A........... 22 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 6797144 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 3,820 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96863.102 (IDP96863.1-82.F..pMCSG68)
MGKFVVVLGDVTSHGGKVTSASSSFDIFGNNAALLNDTVSCPKHGMNKIIECDVSAYEENGRGIVLHGCKTQCGASPDMEVG
ATGGGGAAGTTTGTCGTCGTTTTAGGGGACGTGACTTCGCACGGTGGCAAAGTCACATCAGCCTCATCCAGCTTCGATATATTCGGTAATAATGCCGCACTTTTGAATGATACCGTAAGTTGCCCGAAACACGGTATGAATAAAATTATTGAATGCGATGTTTCTGCGTATGAAGAAAACGGGCGTGGGATTGTATTGCATGGCTGTAAGACACAGTGTGGTGCGAGTCCGGATATGGAGGTTGGC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,736 total letters Query= IDP96863.102 Length=651 Score E Sequences producing significant alignments: (Bits) Value 1_01B_B1_IDP96863.102_T7S.seq 1043 0.0 2_07B_B1_IDP96863.102_T7term.seq 980 0.0 0_wt 444 2e-128 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTC-TAGAAATAATTTTGTTTAACTTTA 89 1 8 ......C.N...................... 38 2 580 .................................................................-........................ 492 2 513 ...G...........T.T.C.C.C...........A-... 551 * E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E Query_1 90 AGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAA 179 1 39 .......................................................................................... 128 2 491 .......................................................................................... 402 N L Y F Q S N A M G K F V V V L G D V T S H G G K V T S A S Query_1 180 CCTGTACTTCCAATCCAATGCCATGGGGAAGTTTGTCGTCGTTTTAGGGGACGTGACTTCGCACGGTGGCAAAGTCACATCAGCCTCATC 269 1 129 .......................................................................................... 218 2 401 .......................................................................................... 312 0 1 .................................................................... 68 S S F D I F G N N A A L L N D T V S C P K H G M N K I I E C Query_1 270 CAGCTTCGATATATTCGGTAATAATGCCGCACTTTTGAATGATACCGTAAGTTGCCCGAAACACGGTATGAATAAAATTATTGAATGCGA 359 1 219 .......................................................................................... 308 1 989 ........... 979 2 311 .......................................................................................... 222 0 69 .......................................................................................... 158 D V S A Y E E N G R G I V L H G C K T Q C G A S P D M E V G Query_1 360 TGTTTCTGCGTATGAAGAAAACGGGCGTGGGATTGTATTGCATGGCTGTAAGACACAGTGTGGTGCGAGTCCGGATATGGAGGTTGGCTA 449 1 309 .......................................................................................... 398 2 221 .......................................................................................... 132 0 159 ........................................................................................ 246 * H W K W I T D P N S S S V D K L A A A L E H H H H H H * D Query_1 450 ACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTG-AGATC 538 1 399 ................................................................---.................-..... 484 2 131 ................................................................---.................A..... 45 P A A N K A R K E A E L A A A T A E Q * L A * P L G A S K Query_1 539 CGGCTGCTAAC--AAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACG 626 1 485 ...........--............................................................................. 572 2 44 ...........AA........... 21 R V L R G F L L K Query_1 627 GGTCTTGAGGGGTTTTTTGCTGAAA 651 1 573 ......................... 597 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1711476 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 2,736 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96864.103 (IDP96864.185-1411.P(1411)..pMCSG68)
SIAEKLGGGILSVLTFASNRTRRDDTWVDEGEYEDDEEEYDDEEAARPQESRRARILRSALARRKRLAEKFTNPMGRKTDAALFSGKRMDDGEEVVQYSASGAPVAADDVLFSGASAARPAEDDVLFSGASAVRPGDFDPYDPLLNGHSIAEPVSAAAAATAAPQAWAESPVGHHGAAPAYQPEASYPPQQAYQPEPAPFQQAYQPEPAPFQQAAYQPPAGQTAPQAYQPEPAPYQQPVYDPRAGQPAPQAYQPEPAPYQQPAYDPYAGQPAPQAYQPEPAPYQQPAYDPHAGQPAPQAYQPEPAPYQQPAYDPYAGQPAPQAYQPEPAPYQQPTYDPYAGQPAPQTYQQPAYDPNAGQPAPQPYQPEPAAYQPQSAPVPPPEPEPEVVQEEVKRPPLYYFEEVEEKRARERELLASWYQPIPEPESPIATKPLTPPTTASKPPVETTVVSAVAAGVHQATAASGGAAAATSSTAASAAATPLFSPASSGPRVQVKEGIGPKLPRPNRVRVPTRRELASYGIKLPSQREAEQRARQAERDPHYDDELLSDEEADAMEQDELARQFAATQQQRYGHRWEDDNATDDDEADAAAEAELARQFAATQQQRYATEQPPGANPFSPADYEFSPMKTLVNDGPSEPLFTPTPEVQPQQPAQRYQQPAAAPQQGYQPAQHQPIHHQPVPPQPQSYPTASQPVQPQQPVAPQGHQPAAPAPQESLIHPLLMRNGDSRPLQKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGDPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWSVNEMERRYKLMSALGVRNLAGYNEKIAEAARMGRPIPDPYWKPGDSMDAVHPVLEKLPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILDQGGAESLLGMGDMLYSGPNSTTPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSDSESEGGGGGFDGGEELDPLFDQAVNFVTEKRKASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFE
AGCATTGCGGAAAAGCTGGGCGGCGGCATCCTGTCCGTTCTCACCTTTGCCAGCAACCGTACCCGTCGGGATGATACCTGGGTCGATGAAGGCGAATATGAAGACGACGAGGAAGAGTACGACGACGAAGAGGCGGCCAGGCCGCAGGAATCGCGTCGCGCCCGTATCTTACGCAGCGCGCTGGCGCGGCGTAAGCGTCTGGCCGAGAAGTTTACCAACCCTATGGGGCGTAAAACCGACGCTGCGCTTTTCTCCGGCAAACGGATGGATGACGGCGAAGAGGTGGTGCAATACAGCGCCAGCGGGGCGCCTGTTGCCGCCGACGATGTACTGTTTTCCGGCGCCAGCGCCGCGCGTCCCGCAGAGGATGATGTGCTGTTCTCCGGCGCCAGCGCCGTGCGCCCGGGCGATTTCGACCCTTACGATCCGTTGTTGAATGGCCACAGTATCGCTGAGCCGGTAAGCGCAGCGGCGGCGGCTACAGCCGCGCCGCAGGCGTGGGCAGAATCACCGGTGGGCCATCACGGCGCTGCGCCAGCTTATCAGCCGGAAGCCAGCTATCCGCCGCAGCAGGCCTATCAGCCTGAACCCGCTCCGTTCCAGCAGGCCTATCAGCCTGAACCCGCTCCGTTCCAGCAGGCTGCTTATCAGCCGCCAGCGGGGCAAACCGCACCGCAGGCGTATCAGCCTGAGCCAGCGCCGTATCAACAGCCGGTTTACGATCCGCGTGCCGGTCAACCTGCGCCGCAGGCCTATCAGCCTGAGCCAGCGCCGTATCAGCAGCCGGCTTACGATCCGTATGCCGGTCAACCTGCGCCGCAGGCCTATCAGCCTGAACCTGCGCCGTATCAGCAGCCGGCTTACGATCCGCATGCCGGTCAACCTGCACCGCAGGCCTATCAGCCTGAGCCAGCGCCGTATCAGCAGCCGGCTTACGATCCCTATGCCGGTCAACCTGCGCCGCAGGCCTATCAGCCGGAGCCAGCGCCGTATCAGCAGCCAACTTACGATCCCTATGCCGGTCAGCCTGCGCCTCAGACCTATCAGCAGCCGGCTTACGATCCGAATGCCGGTCAGCCCGCGCCGCAGCCGTATCAGCCGGAGCCAGCGGCGTATCAGCCGCAAAGCGCGCCAGTTCCCCCACCGGAGCCAGAGCCCGAGGTCGTGCAGGAGGAAGTGAAACGTCCGCCGCTCTATTATTTCGAGGAAGTGGAAGAGAAGCGGGCGCGCGAACGCGAGCTGTTGGCCTCCTGGTATCAGCCAATTCCTGAGCCGGAAAGTCCGATTGCCACTAAACCGCTGACGCCGCCGACCACTGCGTCCAAACCGCCAGTGGAGACAACCGTAGTCTCTGCGGTAGCGGCTGGGGTGCATCAGGCTACCGCCGCCAGCGGCGGCGCGGCGGCAGCAACCTCGTCCACTGCCGCATCCGCTGCGGCTACGCCATTGTTCAGCCCGGCGTCCAGCGGCCCAAGGGTTCAGGTGAAAGAGGGCATCGGTCCAAAACTACCGCGGCCCAATCGCGTGCGTGTTCCTACGCGTCGGGAACTGGCCTCCTACGGCATCAAGCTACCGTCGCAGCGGGAGGCGGAACAGCGCGCGCGGCAGGCGGAGCGCGATCCGCATTATGATGATGAGCTGCTCTCGGATGAGGAAGCGGATGCTATGGAGCAGGATGAACTGGCTCGCCAGTTCGCCGCCACCCAGCAGCAGCGCTACGGTCATCGCTGGGAAGACGATAACGCGACTGATGACGATGAGGCCGACGCCGCGGCGGAAGCGGAGCTGGCGCGTCAGTTTGCCGCTACCCAGCAGCAGCGGTACGCTACCGAGCAGCCGCCGGGCGCCAACCCGTTCTCGCCGGCAGATTATGAATTCTCGCCGATGAAAACGTTGGTCAATGACGGCCCGAGCGAACCGCTGTTTACGCCGACGCCGGAAGTCCAGCCGCAGCAGCCGGCCCAGCGCTATCAACAACCGGCGGCCGCTCCGCAGCAGGGTTATCAACCTGCGCAGCATCAGCCGATACACCATCAGCCTGTGCCGCCACAGCCGCAGTCCTATCCGACTGCGTCGCAGCCCGTACAGCCGCAACAACCGGTTGCCCCGCAGGGGCATCAGCCTGCCGCCCCTGCGCCGCAGGAGAGCCTGATCCACCCGCTGCTGATGCGCAATGGCGATAGTCGACCGCTGCAAAAGCCGACCACGCCACTGCCGTCGCTGGATCTGCTTACCCCGCCGCCGAGTGAAGTCGAGCCGGTGGATACCTTTGCTCTCGAGCAGATGGCACGCCTGGTGGAAGCGCGACTCGCTGATTTCCGCATTAAAGCGGATGTGGTGAACTACTCACCGGGGCCGGTGATCACCCGCTTCGAACTGAATCTGGCGCCTGGCGTTAAGGCCGCACGGATCTCTAACCTGTCACGGGACCTGGCGCGATCGCTGTCAACGGTCGCCGTGCGCGTGGTGGAGGTGATCCCGGGCAAACCGTATGTCGGGCTTGAGCTGCCGAATAAAAAACGCCAGACCGTCTACCTGCGTGAAGTGCTCGACAACGCCAAGTTCCGTGATAACCCATCTCCGCTCACCGTGGTGTTGGGTAAAGACATCGCTGGCGATCCGGTAGTAGCCGATCTGGCGAAAATGCCGCATCTGCTGGTGGCCGGTACCACCGGTTCCGGTAAGTCTGTTGGCGTCAACGCCATGATCCTCAGCATGCTCTACAAGGCGCAGCCGGAAGATGTGCGTTTCATTATGATCGACCCGAAAATGCTCGAGCTGTCGGTCTACGAAGGAATTCCGCACCTGCTGACGGAAGTGGTCACCGACATGAAAGACGCCGCCAATGCGCTGCGCTGGAGCGTCAATGAGATGGAGCGCCGCTACAAGCTGATGTCGGCGCTGGGCGTGCGTAACCTCGCGGGCTACAACGAGAAGATCGCCGAAGCCGCGCGCATGGGACGTCCGATCCCGGATCCGTACTGGAAGCCTGGCGACAGCATGGACGCCGTACATCCGGTGCTGGAAAAACTGCCGTACATCGTGGTGCTGGTGGATGAATTCGCCGATCTGATGATGACCGTCGGCAAAAAGGTGGAAGAGCTGATCGCTCGCCTGGCGCAGAAAGCGCGCGCGGCGGGGATCCACCTGGTGCTGGCGACACAGCGTCCGTCGGTAGATGTTATTACCGGCCTGATTAAGGCCAACATCCCGACGCGCATCGCCTTTACCGTGTCGAGTAAAATTGACTCACGTACCATTCTCGATCAGGGCGGCGCGGAATCGCTGCTGGGTATGGGGGATATGCTTTACTCCGGGCCGAACTCTACCACGCCGGTGCGTGTCCACGGGGCGTTTGTGCGCGACCAGGAAGTCCACGCCGTGGTTCAGGACTGGAAAGCCCGCGGTCGCCCGCAATATGTGGATGGCATTACCTCCGACAGCGAAAGCGAAGGCGGCGGTGGCGGCTTCGACGGCGGGGAAGAGTTGGATCCGTTGTTCGATCAGGCAGTCAACTTTGTGACCGAGAAGCGCAAAGCGTCGATTTCCGGGGTTCAGCGTCAGTTCCGCATCGGCTATAACCGTGCCGCGCGTATTATCGAACAGATGGAAGCGCAGGGTATCGTCAGCGAGCAGGGCCATAACGGTAACCGCGAAGTGCTGGCGCCGCCGCCCTTTGAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,691 total letters Query= IDP96864.103 Length=4086 Score E Sequences producing significant alignments: (Bits) Value 0_wt 6639 0.0 S I A E K L G G G I L S V L T F A S N R T R R D D T W V D E Query_1 202 AGCATTGCGGAAAAGCTGGGCGGCGGCATCCTGTCCGTTCTCACCTTTGCCAGCAACCGTACCCGTCGGGATGATACCTGGGTCGATGAA 291 0 1 .......................................................................................... 90 G E Y E D D E E E Y D D E E A A R P Q E S R R A R I L R S A Query_1 292 GGCGAATATGAAGACGACGAGGAAGAGTACGACGACGAAGAGGCGGCCAGGCCGCAGGAATCGCGTCGCGCCCGTATCTTACGCAGCGCG 381 0 91 .......................................................................................... 180 L A R R K R L A E K F T N P M G R K T D A A L F S G K R M D Query_1 382 CTGGCGCGGCGTAAGCGTCTGGCCGAGAAGTTTACCAACCCTATGGGGCGTAAAACCGACGCTGCGCTTTTCTCCGGCAAACGGATGGAT 471 0 181 .......................................................................................... 270 D G E E V V Q Y S A S G A P V A A D D V L F S G A S A A R P Query_1 472 GACGGCGAAGAGGTGGTGCAATACAGCGCCAGCGGGGCGCCTGTTGCCGCCGACGATGTACTGTTTTCCGGCGCCAGCGCCGCGCGTCCC 561 0 271 .......................................................................................... 360 0 653 ............ 664 A E D D V L F S G A S A V R P G D F D P Y D P L L N G H S I Query_1 562 GCAGAGGATGATGTGCTGTTCTCCGGCGCCAGCGCCGTGCGCCCGGGCGATTTCGACCCTTACGATCCGTTGTTGAATGGCCACAGTATC 651 0 361 .......................................................................................... 450 0 788 ............------...---C.G..CAA 810 0 976 ....A........... 991 0 693 ........... 703 0 765 ........... 775 0 909 ........... 919 0 3490 ........... 3500 A E P V S A A A A A T A A P Q A W A E S P V G H H G A A P Query_1 652 GCTGAGCCGGTAAGCGCAGCGGCGGCGGCTACAGCCGCGCCG---CAGGCGTGGGCAGAATCACCGGTGGGCCATCACGGCGCTGCGCCA 738 0 451 ..........................................---............................................. 537 0 811 C...C...-.C.G..CT.T.A..-----..GA.C.T......TAT...CA.CC...TT.CGAT...CAT.C.GG...ACCT..AC...AG 894 0 783 ...G 786 0 855 ...G 858 0 1079 ........---...C... 1093 0 742 ......---..... 752 0 958 ......---..... 968 0 1389 ........... 1399 A Y Q P E A S Y P P Q Q A Y Q P E P A P F Q Q Query_1 739 GCTTATCAGCCGGAAGCCAGC----TATCCG---CCGCAGCAGGCCTATCAGCCTGAACCCGCTC--CGTTC-CAGCAG----------- 807 0 538 .....................----......---...............................--.....-......----------- 606 0 747 ....----------- 750 0 819 ....----------- 822 0 891 ....----------- 894 0 895 ..C........T.-.......------G...TAT.A.....C...T..CG.T..CT.TG...G..AA.C.G.G.C....----------- 966 0 959 ...C...............G..G..A..G.--...AT-......CCAACTTACGA 1010 0 787 .....CG.T...T.T...G.T----C.A..T---G...C.....................T..G.--... 847 0 859 .....CG.T...C.T...G.T----C.A..T---G.A.C..................G..A..G.--... 919 0 743 ...C..................G..A..G.--... 775 0 967 ..C..........-....... 986 0 1093 ..........-.......GGCG....A.---..... 1124 A Y Q P E P A P F Q Q A A Y Q P P A G Q T A P Q A Y Q Query_1 808 -------GCCTATCAGCCTGAACCCGCTCCGTTCCAGCAGGCTGCTTATCAGCCGCCAGCGGGGCAAACCGCACCGCAGGCGTATCAGCC 890 0 607 -------................................................................................... 689 0 751 -------..............G..A..G....AT......C.G.....CG.T...TAT..C..T...C.T..G........C........ 833 0 823 -------.................T..G....AT......C.G.....CG.T....AT..C..T...C.T...........C........ 905 0 895 -------..............G..A..G....AT......C.G.....CG.T..CTAT..C..T...C.T..G........C........ 977 0 967 -------...........G..G..A..G....AT......C.AA....---------------------..AT..CT.T..CGG...... 1028 0 1011 TCCCTAT...GG........CG..TCAGA.C.AT......C.G.....CG.T...AAT..C..T..GC....G......C.......... 1100 0 1945 ...C..C.G..... 1958 0 297 ............ 308 0 1110 ............ 1121 0 1384 ........... 1394 P E P A P Y Q Q P V Y D P R A G Q P A P Q A Y Q P E P A P Query_1 891 TGAGCCAGCGCCGTATCAACAGCCGGTTTACGATCCGCGTGCCGG----TCAACCTGCGCCGCAGGCCTATCAGCCTGAGCCAGCGCCGT 976 0 690 .............................................----......................................... 775 0 603 ..................A..C..T.... 631 0 834 ...A..T...........G.......C...........A......----.........A............................... 919 0 906 ..................G.......C.........CTA......----...........................G............. 991 0 978 G.................G.....AAC.........CTA......----...G........T...A..---------------------. 1042 0 1029 ..C...TCA.A.C.....G.......C..........AA......----...G..C.........C.G........G.........G... 1114 0 1101 G.........G....... 1118 0 566 ...A..................A..C..T.... 598 0 1959 G.C-.......--........A....CGGC..C.....A-..A..GTTA...........A... 2018 0 1962 .......--. 1969 0 2131 ............. 2143 0 487 ........... 497 Y Q Q P A Y D P Y A G Q P A P Q A Y Q P E P A P Y Q Q P A Query_1 977 ATCAGCAGCCGGCTTACGATCCGTATGCCGG----TCAACCTGCGCCGCAGGCCTATCAGCCTGAACCTGCGCCGTATCAGCAGCCGGCT 1062 0 776 ...............................----....................................................... 861 0 632 TC......G.T.....TC.G...CCA..G..----G...A.C..A........G...........G..A...........A.......T. 717 0 920 ......................C........----...........................G..G..A.................AA.. 1005 0 603 .....................C..T....TC......G.T... 645 0 992 ..........AA..........C........----...G........T...A..---------------------............... 1056 0 1043 .......................A.......----...G..C.........C.G........G..G..A...G.........C.. 1123 0 1115 ......C.. 1123 0 534 ...A.....TC.G...G.A...A.----CT.T..GC...A.....................C..T.... 598 0 534 ...A... 540 0 1970 ....A..A.....GGC..C....C.-..A..GTTA...........A... 2018 0 2131 ............. 2143 0 487 ........... 497 Y D P H A G Q P A P Q A Y Q P E P A P Y Q Q P A Y D P Y A Query_1 1063 TACGATCCGCATGCCGGTCAACCTGCACCGCAGGCCTATCAGCCTGAGCCAGCGCCGTATCAGCAGCCGGCTTACGATCCCTATGCCGG- 1151 0 862 .........................................................................................- 950 0 718 ..........G...............G.....................................................G........- 806 0 1006 ........CT..........G.....G..T...A..---------------------.......................GA.......- 1073 0 646 ..TC.G....CA..G..G...A.C...........G..........................A.......T.........GCG......- 734 0 1057 .........A..........G..C..G......C.G........G.........G.........C.. 1123 0 603 ..................A..C..T....TC......G.T.....TC.G..GCCA..G..- 662 0 541 ..TC.G...G.A...A.CT.T..GC.G.A..................A..C..T.... 598 0 1962 .......--.....A..A.....GGC..C...GC.-..A..G 2000 G Q P A P Q A Y Q P E P A P Y Q Q P T Y D P Y A G Q P A P Query_1 1152 ---TCAACCTGCGCCGCAGGCCTATCAGCCGGAGCCAGCGCCGTATCAGCAGCCAACTTACGATCCCTATGCCGGTCAGCCTGCGCCTCA 1238 0 951 ---....................................................................................... 1037 0 807 ---...........................T..A..T.................GG..........GC..........A.....A..G.. 893 0 1074 ---...G..C.........C.G..................G.........C..A.. 1126 0 735 ---...........................T.......................GG..........G...........A........G.. 821 0 663 ---G...A.C..A........G........T.................A.....GGT.........GCG.........A........G.. 749 0 566 ...A...............T..A..C..T....TC......------------------...TA........AA..CGC 626 0 2001 TTA...........A... 2018 0 383 ....C........... 398 0 2131 ............. 2143 0 487 ........... 497 Q T Y Q Q P A Y D P N A G Q P A P Q P Y Q P E Query_1 1239 GACC---------------------TATCAGCAGCCGGCTTACGATCCGAATGCCGGTCAGCCCGCGCCGCAGCCGTATCAGCCGG-AGC 1306 0 1038 ....---------------------.............................................................-... 1105 0 894 .G..TATCAGCCTGAGCCAGCGCCG.......................CT..........A..T.........G.C..........-... 982 0 822 .G..TATCAGCCTGAACCTGCGCCG........................C..........A..T..A......G.C........T.-... 910 0 750 .G..TATCAGCCTGAGCCAGCGCCG........................T..........A..T.........G.C........T.-.A. 838 0 627 TC.G---------------------.TC......G.T.....TC.G...CCA..G..G..AA....A......G..........T.-... 694 0 485 ...........G... 499 0 541 ..........A... 554 P A A Y Q P Q S A P V P P P E P E P E V V Q E E V K R P P L Query_1 1307 CAGCGGCGTATCAGCCGCAAAGCGCGCCAGTTCCCCCACCGGAGCCAGAGCCCGAGGTCGTGCAGGAGGAAGTGAAACGTCCGCCGCTCT 1396 0 1106 .......................................................................................... 1195 0 983 .....C.........A..C.. 1003 0 911 .....C.........A.. 928 0 839 .T...C.........A.. 856 0 695 .....C....... 707 0 678 ............ 689 0 555 ....----....C...... 569 Y Y F E E V E E K R A R E R E L L A S W Y Q P I P E P E S P Query_1 1397 ATTATTTCGAGGAAGTGGAAGAGAAGCGGGCGCGCGAACGCGAGCTGTTGGCCTCCTGGTATCAGCCAATTCCTGAGCCGGAAAGTCCGA 1486 0 1196 .......................................................................................... 1285 I A T K P L T P P T T A S K P P V E T T V V S A V A A G V H Query_1 1487 TTGCCACTAAACCGCTGACGCCGCCGACCACTGCGTCCAAACCGCCAGTGGAGACAACCGTAGTCTCTGCGGTAGCGGCTGGGGTGCATC 1576 0 1286 .......................................................................................... 1375 Q A T A A S G G A A A A T S S T A A S A A A T P L F S P A S Query_1 1577 AGGCTACCGCCGCCAGCGGCGGCGCGGCGGCAGCAACCTCGTCCACTGCCGCATCCGCTGCGGCTACGCCATTGTTCAGCCCGGCGTCCA 1666 0 1376 .......................................................................................... 1465 0 651 ........... 661 S G P R V Q V K E G I G P K L P R P N R V R V P T R R E L A Query_1 1667 GCGGCCCAAGGGTTCAGGTGAAAGAGGGCATCGGTCCAAAACTACCGCGGCCCAATCGCGTGCGTGTTCCTACGCGTCGGGAACTGGCCT 1756 0 1466 .......................................................................................... 1555 S Y G I K L P S Q R E A E Q R A R Q A E R D P H Y D D E L L Query_1 1757 CCTACGGCATCAAGCTACCGTCGCAGCGGGAGGCGGAACAGCGCGCGCGGCAGGCGGAGCGCGATCCGCATTATGATGATGAGCTGCTCT 1846 0 1556 .......................................................................................... 1645 0 3126 ............G.. 3140 S D E E A D A M E Q D E L A R Q F A A T Q Q Q R Y G H R W E Query_1 1847 CGGATGAGGAAGCGGATGCTATGGAGCAGGATGAACTGGCTCGCCAGTTCGCCGCCACCCAGCAGCAGCGCTACGGTCATCGCTGGGAAG 1936 0 1646 .......................................................................................... 1735 0 1783 ..G.....G..T.....T.....T..............G.... 1825 D D N A T D D D E A D A A A E A E L A R Q F A A T Q Q Q R Y Query_1 1937 ACGATAACGCGACTGATGACGATGAGGCCGACGCCGCGGCGGAAGCGGAGCTGGCGCGTCAGTTTGCCGCTACCCAGCAGCAGCGGTACG 2026 0 1736 .......................................................................................... 1825 0 1678 ..A.....T..C.....C.....C..............C.... 1720 0 3295 ........... 3285 A T E Q P P G A N P F S P A D Y E F S P M K T L V N D G P S Query_1 2027 CTACCGAGCAGCCGCCGGGCGCCAACCCGTTCTCGCCGGCAGATTATGAATTCTCGCCGATGAAAACGTTGGTCAATGACGGCCCGAGCG 2116 0 1826 .......................................................................................... 1915 E P L F T P T P E V Q P Q Q P A Q R Y Q Q P A A A P Q Q G Query_1 2117 AACCGCTGTTTACGCCGACGCCGGAAGTCCAGCCGCAGCAGCCGGC-CCAGCGC--TATCAACAACCGGCGGCCGCTCCGCA-GCAGGGT 2202 0 1916 ..............................................-.......--..........................-....... 2001 0 676 ...G..T.T.....T.AG.......CG........G....TTTA..A.....GT..C..-- 734 0 766 .......CG.....G..G.....TTA..A....T.T..C..-- 806 0 910 .......CG.....G..G.....TTA..A...CT.T..C..-- 950 0 778 ........... 788 0 850 ........... 860 0 922 ........... 932 0 1045 ........... 1055 Y Q P A Q H Q P I H H Q P V P P Q P Q S Y P T A S Q P V Q P Query_1 2203 TATCAACCTGCGCAGCATCAGCCGATACACCATCAGCCTGTGCCGCCACAGCCGCAGTCCTATCCGACTGCGTCGCAGCCCGTACAGCCG 2292 0 2002 .......................................................................................... 2091 0 735 --...........C... 749 0 807 --...........C... 821 0 951 --...........C... 965 Q Q P V A P Q G H Q P A A P A P Q E S L I H P L L M R N G D Query_1 2293 CAACAACCGGTTGCCCCGCAGGGGCATCAGCCTGCCGCCCCTGCGCCGCAGGAGAGCCTGATCCACCCGCTGCTGATGCGCAATGGCGAT 2382 0 2092 .......................................................................................... 2181 0 739 ............. 751 0 811 ............. 823 0 955 ............. 967 S R P L Q K P T T P L P S L D L L T P P P S E V E P V D T F Query_1 2383 AGTCGACCGCTGCAAAAGCCGACCACGCCACTGCCGTCGCTGGATCTGCTTACCCCGCCGCCGAGTGAAGTCGAGCCGGTGGATACCTTT 2472 0 2182 .......................................................................................... 2271 A L E Q M A R L V E A R L A D F R I K A D V V N Y S P G P V Query_1 2473 GCTCTCGAGCAGATGGCACGCCTGGTGGAAGCGCGACTCGCTGATTTCCGCATTAAAGCGGATGTGGTGAACTACTCACCGGGGCCGGTG 2562 0 2272 .......................................................................................... 2361 I T R F E L N L A P G V K A A R I S N L S R D L A R S L S T Query_1 2563 ATCACCCGCTTCGAACTGAATCTGGCGCCTGGCGTTAAGGCCGCACGGATCTCTAACCTGTCACGGGACCTGGCGCGATCGCTGTCAACG 2652 0 2362 .......................................................................................... 2451 V A V R V V E V I P G K P Y V G L E L P N K K R Q T V Y L R Query_1 2653 GTCGCCGTGCGCGTGGTGGAGGTGATCCCGGGCAAACCGTATGTCGGGCTTGAGCTGCCGAATAAAAAACGCCAGACCGTCTACCTGCGT 2742 0 2452 .......................................................................................... 2541 E V L D N A K F R D N P S P L T V V L G K D I A G D P V V A Query_1 2743 GAAGTGCTCGACAACGCCAAGTTCCGTGATAACCCATCTCCGCTCACCGTGGTGTTGGGTAAAGACATCGCTGGCGATCCGGTAGTAGCC 2832 0 2542 .......................................................................................... 2631 D L A K M P H L L V A G T T G S G K S V G V N A M I L S M L Query_1 2833 GATCTGGCGAAAATGCCGCATCTGCTGGTGGCCGGTACCACCGGTTCCGGTAAGTCTGTTGGCGTCAACGCCATGATCCTCAGCATGCTC 2922 0 2632 .......................................................................................... 2721 Y K A Q P E D V R F I M I D P K M L E L S V Y E G I P H L L Query_1 2923 TACAAGGCGCAGCCGGAAGATGTGCGTTTCATTATGATCGACCCGAAAATGCTCGAGCTGTCGGTCTACGAAGGAATTCCGCACCTGCTG 3012 0 2722 .......................................................................................... 2811 T E V V T D M K D A A N A L R W S V N E M E R R Y K L M S A Query_1 3013 ACGGAAGTGGTCACCGACATGAAAGACGCCGCCAATGCGCTGCGCTGGAGCGTCAATGAGATGGAGCGCCGCTACAAGCTGATGTCGGCG 3102 0 2812 .......................................................................................... 2901 L G V R N L A G Y N E K I A E A A R M G R P I P D P Y W K P Query_1 3103 CTGGGCGTGCGTAACCTCGCGGGCTACAACGAGAAGATCGCCGAAGCCGCGCGCATGGGACGTCCGATCCCGGATCCGTACTGGAAGCCT 3192 0 2902 .......................................................................................... 2991 G D S M D A V H P V L E K L P Y I V V L V D E F A D L M M T Query_1 3193 GGCGACAGCATGGACGCCGTACATCCGGTGCTGGAAAAACTGCCGTACATCGTGGTGCTGGTGGATGAATTCGCCGATCTGATGATGACC 3282 0 2992 .......................................................................................... 3081 V G K K V E E L I A R L A Q K A R A A G I H L V L A T Q R P Query_1 3283 GTCGGCAAAAAGGTGGAAGAGCTGATCGCTCGCCTGGCGCAGAAAGCGCGCGCGGCGGGGATCCACCTGGTGCTGGCGACACAGCGTCCG 3372 0 3082 .......................................................................................... 3171 0 1595 ............A.. 1609 S V D V I T G L I K A N I P T R I A F T V S S K I D S R T I Query_1 3373 TCGGTAGATGTTATTACCGGCCTGATTAAGGCCAACATCCCGACGCGCATCGCCTTTACCGTGTCGAGTAAAATTGACTCACGTACCATT 3462 0 3172 .......................................................................................... 3261 L D Q G G A E S L L G M G D M L Y S G P N S T T P V R V H G Query_1 3463 CTCGATCAGGGCGGCGCGGAATCGCTGCTGGGTATGGGGGATATGCTTTACTCCGGGCCGAACTCTACCACGCCGGTGCGTGTCCACGGG 3552 0 3262 .......................................................................................... 3351 0 1816 ........... 1806 A F V R D Q E V H A V V Q D W K A R G R P Q Y V D G I T S D Query_1 3553 GCGTTTGTGCGCGACCAGGAAGTCCACGCCGTGGTTCAGGACTGGAAAGCCCGCGGTCGCCCGCAATATGTGGATGGCATTACCTCCGAC 3642 0 3352 .......................................................................................... 3441 S E S E G G G G G F D G G E E L D P L F D Q A V N F V T E K Query_1 3643 AGCGAAAGCGAAGGCGGCGGTGGCGGCTTCGACGGCGGGGAAGAGTTGGATCCGTTGTTCGATCAGGCAGTCAACTTTGTGACCGAGAAG 3732 0 3442 .......................................................................................... 3531 R K A S I S G V Q R Q F R I G Y N R A A R I I E Q M E A Q G Query_1 3733 CGCAAAGCGTCGATTTCCGGGGTTCAGCGTCAGTTCCGCATCGGCTATAACCGTGCCGCGCGTATTATCGAACAGATGGAAGCGCAGGGT 3822 0 3532 .......................................................................................... 3621 I V S E Q G H N G N R E V L A P P P F E * H W K W I T D P N Query_1 3823 ATCGTCAGCGAGCAGGGCCATAACGGTAACCGCGAAGTGCTGGCGCCGCCGCCCTTTGAATAACATTGGAAGTGGATAACGGATCCGAAT 3912 0 3622 ............................................................ 3681 S S S V D K L A A A L E H H H H H H * D P A A N K A R K E A Query_1 3913 TCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCT 4002 E L A A Query_1 4003 GAGTTGGCTGCTG 4015 0 1005 ............ 994 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 14811160 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 3,691 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96865.103 (IDP96865.1-623.F..pMCSG73)
MNINEKTPVGEVRPSQLLWTYGPGALIDLPNLSVVTLGIDRWEKNCCQPIQETRLLAAVRKVLGDQVESLRMPPFQKSEVTDMWSAEANIGVPVRPFPRWLRCVKCGLLSPYDAGLFEIKENRFRPERTRFVHKDCRGSKGDQPARDADAVPARFLLACRDGHLDDFPWHYFVHGGNNPCKGTLRFFESGASLQTENLWVKCDSCELSRSMAHAFGKAGKENLPACRGRHPHLDYFDAECDEEPRAVLLGATNSWFPVTLSALAIPLSNVTPLHQIIIDYWDDFSDVEDLSELKGILKILNKRGEAQGIEKHAAEEIWSAIETHRSQDGEDFVGEADIKDPEWNVLIEPTPPTDYPHFMSKKTRTPTRFAPYIERVLLLERLREVNALLGFTRVEAPEESEDLNERPQMASLSRSKPDWVPANQVHGEGIFLQFNEQALVAWESLDAVKQSDKMLRGGHTGWRNARHLDPEEGYPGIRYAMLHTLSHLLIRELALECGYNAASIRERIYANTTNGTPQAGILIYTAAADSDGTLGGLVDLGKPENLGRLLEQALNRSRICSSDPLCSEHDPEKDRSLHGAACHACSLVAETSCEKGNRYLDRALLVPTLEHTEAAFFKDLLNG
ATGAACATTAATGAGAAAACACCCGTTGGTGAAGTTCGCCCGAGCCAATTGCTTTGGACATATGGTCCTGGAGCCCTAATTGATTTGCCCAACTTGTCAGTTGTAACACTTGGGATCGACAGGTGGGAAAAGAACTGTTGTCAGCCAATTCAGGAAACCCGGTTACTAGCTGCCGTTAGAAAGGTATTAGGTGATCAGGTTGAAAGCTTGCGAATGCCACCATTTCAAAAAAGCGAAGTCACAGATATGTGGTCTGCTGAAGCAAATATAGGGGTACCTGTACGTCCTTTCCCTAGATGGTTGCGATGCGTTAAATGTGGGTTACTTTCACCTTACGATGCCGGGCTTTTCGAAATAAAAGAAAATCGATTTAGGCCTGAACGAACTCGCTTTGTTCATAAAGACTGCCGGGGTTCTAAGGGCGATCAACCTGCAAGGGATGCTGATGCAGTTCCAGCCCGTTTTCTCTTGGCTTGCCGTGATGGTCATCTTGATGATTTTCCGTGGCACTATTTTGTTCATGGTGGTAACAATCCATGCAAAGGAACGCTGCGATTTTTTGAAAGTGGCGCTTCATTGCAGACAGAAAATCTTTGGGTAAAATGCGATTCCTGTGAATTATCAAGAAGTATGGCGCATGCATTTGGTAAGGCCGGTAAAGAAAATCTTCCCGCATGTAGAGGACGTCATCCTCATCTGGATTACTTTGATGCTGAATGTGACGAAGAACCTCGTGCAGTACTGCTTGGGGCTACGAATAGCTGGTTTCCTGTAACGCTATCAGCACTAGCGATCCCATTATCAAATGTTACTCCATTACATCAGATAATCATTGATTACTGGGATGATTTTTCTGATGTAGAAGATCTTTCTGAACTTAAAGGGATACTAAAAATCCTCAACAAACGTGGTGAAGCTCAAGGTATTGAAAAGCATGCTGCAGAGGAAATATGGTCTGCAATTGAAACTCATCGCTCACAAGATGGCGAGGATTTTGTCGGTGAGGCTGATATTAAAGATCCTGAATGGAATGTACTAATAGAACCTACTCCCCCCACTGATTATCCTCATTTTATGAGTAAAAAAACCCGTACTCCTACGCGGTTTGCGCCATATATTGAGCGAGTTTTACTCTTAGAACGATTACGTGAGGTTAATGCTTTACTTGGTTTTACCCGGGTCGAGGCTCCTGAAGAATCTGAAGATCTCAACGAAAGACCGCAAATGGCCAGCCTGTCACGGAGCAAACCTGATTGGGTACCCGCCAATCAGGTCCATGGTGAAGGGATATTCCTGCAGTTCAATGAACAAGCGCTTGTGGCATGGGAATCTCTGGACGCTGTAAAACAATCGGACAAAATGCTTCGCGGTGGGCATACAGGTTGGAGAAATGCTCGTCACTTGGATCCAGAAGAAGGTTATCCCGGCATTCGCTATGCTATGTTGCATACACTTTCCCATTTACTGATTCGAGAATTGGCTCTGGAATGTGGATATAATGCCGCGAGTATTCGTGAGCGTATCTATGCAAATACAACCAATGGTACTCCTCAGGCGGGTATCCTTATTTATACCGCTGCCGCTGATTCAGACGGTACTTTAGGGGGACTGGTTGATCTTGGCAAACCAGAAAATCTTGGACGTTTACTTGAACAAGCATTAAATCGCTCAAGGATCTGCTCATCAGACCCATTATGTTCAGAGCATGATCCAGAGAAGGATCGTTCGCTGCATGGAGCTGCTTGCCATGCCTGTAGCTTAGTTGCAGAAACCTCTTGCGAGAAAGGGAATCGCTATTTAGATAGAGCCCTGCTTGTACCAACACTGGAGCATACTGAAGCAGCTTTCTTCAAGGATTTATTAAATGGA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,301 total letters Query= IDP96865.103 Length=2274 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3371 0.0 2_10B_B4_IDP96865.103_T7_Term.seq 1923 0.0 1_04B_B4_IDP96865.103_v73F.seq 1842 0.0 H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 110 ACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATG 199 1 33 ...........A........G....------........................ 81 1 16 ....C........... 31 A M N I N E K T P V G E V R P S Q L L W T Y G P G A L I D L Query_1 200 CCATGAACATTAATGAGAAAACACCCGTTGGTGAAGTTCGCCCGAGCCAATTGCTTTGGACATATGGTCCTGGAGCCCTAATTGATTTGC 289 0 1 ........................................................................................ 88 1 82 .......................................................................................... 171 P N L S V V T L G I D R W E K N C C Q P I Q E T R L L A A V Query_1 290 CCAACTTGTCAGTTGTAACACTTGGGATCGACAGGTGGGAAAAGAACTGTTGTCAGCCAATTCAGGAAACCCGGTTACTAGCTGCCGTTA 379 0 89 .......................................................................................... 178 1 172 .......................................................................................... 261 R K V L G D Q V E S L R M P P F Q K S E V T D M W S A E A N Query_1 380 GAAAGGTATTAGGTGATCAGGTTGAAAGCTTGCGAATGCCACCATTTCAAAAAAGCGAAGTCACAGATATGTGGTCTGCTGAAGCAAATA 469 0 179 .......................................................................................... 268 1 262 .......................................................................................... 351 I G V P V R P F P R W L R C V K C G L L S P Y D A G L F E I Query_1 470 TAGGGGTACCTGTACGTCCTTTCCCTAGATGGTTGCGATGCGTTAAATGTGGGTTACTTTCACCTTACGATGCCGGGCTTTTCGAAATAA 559 0 269 .......................................................................................... 358 1 352 .......................................................................................... 441 K E N R F R P E R T R F V H K D C R G S K G D Q P A R D A D Query_1 560 AAGAAAATCGATTTAGGCCTGAACGAACTCGCTTTGTTCATAAAGACTGCCGGGGTTCTAAGGGCGATCAACCTGCAAGGGATGCTGATG 649 0 359 .......................................................................................... 448 1 442 .......................................................................................... 531 A V P A R F L L A C R D G H L D D F P W H Y F V H G G N N P Query_1 650 CAGTTCCAGCCCGTTTTCTCTTGGCTTGCCGTGATGGTCATCTTGATGATTTTCCGTGGCACTATTTTGTTCATGGTGGTAACAATCCAT 739 0 449 .......................................................................................... 538 1 532 .......................................................................................... 621 C K G T L R F F E S G A S L Q T E N L W V K C D S C E L S R Query_1 740 GCAAAGGAACGCTGCGATTTTTTGAAAGTGGCGCTTCATTGCAGACAGAAAATCTTTGGGTAAAATGCGATTCCTGTGAATTATCAAGAA 829 0 539 .......................................................................................... 628 0 1628 ........... 1638 2 969 ............ 980 2 308 ........... 298 1 622 .......................................................................................... 711 S M A H A F G K A G K E N L P A C R G R H P H L D Y F D A E Query_1 830 GTATGGCGCATGCATTTGGTAAGGCCGGTAAAGAAAATCTTCCCGCATGTAGAGGACGTCATCCTCATCTGGATTACTTTGATGCTGAAT 919 0 629 .......................................................................................... 718 1 712 .......................................................................................... 801 C D E E P R A V L L G A T N S W F P V T L S A L A I P L S N Query_1 920 GTGACGAAGAACCTCGTGCAGTACTGCTTGGGGCTACGAATAGCTGGTTTCCTGTAACGCTAT-CAGCACTAGCGATCCCATTATCAAAT 1008 0 719 ...............................................................-.......................... 807 2 1159 .....T...NN....N.NN..-......C... 1129 1 802 ...............................................................-......................-... 889 V T P L H Q I I I D Y W D D F S D V E D L S E L K G I L K Query_1 1009 GTT-ACTCCATTACATCAGATAATCATTGATTACTGGGATGATTTTTCTGATGTAGAA-GATCTTTCTGAACTTAAAGGGATACTAAAAA 1096 0 808 ...-......................................................-............................... 895 2 1128 ...T..N......................N....N.........N.............A............................... 1039 2 1064 ..-......... 1074 1 890 ...-......................................................-............................... 977 I L N K R G E A Q G I E K H A A E E I W S A I E T H R S Q Query_1 1097 TCCTCAACAAACGTGGTGAAGCTCAAGGTATTGAAAAGCATGC-TGCAGAGGAAATATGGTCTGCAATTGAAACTCATCGCTCACAAGA- 1184 0 896 ...........................................-.............................................- 983 2 1038 .T.........................................-........................-....................- 952 1 978 ..............-.NN.NCT....N......-.........N...N..A.....-NN...........-...............NN.A 1063 D G E D F V G E A D I K D P E W N V L I E P T P P T D Y P Query_1 1185 -TGGCGAGGATTTTGTCGGTGAGGCTGATA-TTAAAGATCC-TGAATGGAATGTACTAATAGAACCTACT--CCCCCCACTGATTATCCT 1269 0 984 -.............................-..........-............................--.................. 1068 2 951 -............-................-..........-............................--.................. 868 1 1064 TG.....-NN.......-.N..N.......T..........T......N...............-.N...TC...........-NN.... 1149 H F M S K K T R T P T R F A P Y I E R V L L L E R L R E V Query_1 1270 -CATTTTATGAGTaaaaaaaCCCGTACTCCTACGCGGTTTGCGCCATATATTGAGCGAGTTTTACTCTTAGAACGATTACGTGAGGTTAA 1358 0 1069 -......................................................................................... 1157 2 867 -......................................................................................... 779 1 1150 C............-..N.NN...........TN..C.NN....... 1194 N A L L G F T R V E A P E E S E D L N E R P Q M A S L S R S Query_1 1359 TGCTTTACTTGGTTTTACCCGGGTCGAGGCTCCTGAAGAATCTGAAGATCTCAACGAAAGACCGCAAATGGCCAGCCTGTCACGGAGCAA 1448 0 1158 .......................................................................................... 1247 2 778 .......................................................................................... 689 K P D W V P A N Q V H G E G I F L Q F N E Q A L V A W E S L Query_1 1449 ACCTGATTGGGTACCCGCCAATCAGGTCCATGGTGAAGGGATATTCCTGCAGTTCAATGAACAAGCGCTTGTGGCATGGGAATCTCTGGA 1538 0 1248 .......................................................................................... 1337 0 1319 ............ 1308 2 688 .......................................................................................... 599 2 617 ............ 628 D A V K Q S D K M L R G G H T G W R N A R H L D P E E G Y P Query_1 1539 CGCTGTAAAACAATCGGACAAAATGCTTCGCGGTGGGCATACAGGTTGGAGAAATGCTCGTCACTTGGATCCAGAAGAAGGTTATCCCGG 1628 0 1338 .......................................................................................... 1427 2 598 .......................................................................................... 509 G I R Y A M L H T L S H L L I R E L A L E C G Y N A A S I R Query_1 1629 CATTCGCTATGCTATGTTGCATACACTTTCCCATTTACTGATTCGAGAATTGGCTCTGGAATGTGGATATAATGCCGCGAGTATTCGTGA 1718 0 1428 .......................................................................................... 1517 2 508 .......................................................................................... 419 E R I Y A N T T N G T P Q A G I L I Y T A A A D S D G T L G Query_1 1719 GCGTATCTATGCAAATACAACCAATGGTACTCCTCAGGCGGGTATCCTTATTTATACCGCTGCCGCTGATTCAGACGGTACTTTAGGGGG 1808 0 1518 .......................................................................................... 1607 2 418 .......................................................................................... 329 G L V D L G K P E N L G R L L E Q A L N R S R I C S S D P L Query_1 1809 ACTGGTTGATCTTGGCAAACCAGAAAATCTTGGACGTTTACTTGAACAAGCATTAAATCGCTCAAGGATCTGCTCATCAGACCCATTATG 1898 0 1608 .......................................................................................... 1697 0 584 ........... 594 2 328 .......................................................................................... 239 1 667 ........... 677 C S E H D P E K D R S L H G A A C H A C S L V A E T S C E K Query_1 1899 TTCAGAGCATGATCCAGAGAAGGATCGTTCGCTGCATGGAGCTGCTTGCCATGCCTGTAGCTTAGTTGCAGAAACCTCTTGCGAGAAAGG 1988 0 1698 .......................................................................................... 1787 2 238 .......................................................................................... 149 G N R Y L D R A L L V P T L E H T E A A F F K D L L N G * H Query_1 1989 GAATCGCTATTTAGATAGAGCCCTGCTTGTACCAACACTGGAGCATACTGAAGCAGCTTTCTTCAAGGATTTATTAAATGGATAACATTG 2078 0 1788 .................................................................................. 1869 2 148 .......................................................................................... 59 W K W I T D P N S S S V D K L A A A L E H H H H H H * D P A Query_1 2079 GAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGC 2168 2 58 ............--..... 42 2 46 .......... 37 A N K A R K E Query_1 2169 TAACAAAG-CCCGAAAGGAAG 2188 2 36 ........C.......A.... 16 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 9592250 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 4,301 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96868.102 (IDP96868.1-1343.F..pMCSG68)
MSLNNIHAEWLSLVDISGPFLAIPVLTHTFPQGLEVLSSFKRRRLRQAYDEWREALELEDPQLEELHSAWIDEVLSRVLEFDDDGKGDVLKREEWCRSHLKSVLPDQGVVEYPDLAVVDEQQDNKPLLLIHTYKPNVELESTVKYEGWITTPADRMVQLCRSLGCRLGLLTNGECWMLVDAPVGAVTSFSSWYARLWSQEPITLQAFVHLLGIRRFFVDESEQLPALFDASLKYQDEVTDALGEQVCRAVEVLIQSLDKADQDRNRELLHDVNETELYEAALAVMMRLVFLLSAEEQGLLLLGDECYESNYAISTLRMQLRQEPSELLERRWDAWTRLLSTFRAVYGGVEHEELRLPALGSSLFDPDHFPFLEGRTKGSNWKIDTAVPLPINNRTVLLLLESIQLFQGRTLSYRALDVEQIGYVYEGLLERTVKRAAEVTLELNATKKAQSPWVTLAELDSVSMDGTEQLIELLHERSGSSISKIRNDLSKLIDDSLIERLLITCQGDTKLRDRIKPYVHLLRTDPWGYPLVYPAGSFIVTSGSNRRETGTHYTPKSLTEAIVAETLTPLTYIGPTEGIPREKWQLKSSSELLTLKICDPAMGSGAFLVQTCRWLADRLVESWTLAEESGKKISVDGYILGTSSTQEYLPHDTDARIVIARRLIAERCLYGVDLNPLAVELAKLSIWLVTLAKDRPFGFLEHNLRCGDSLLGIERIDQLTQFSMNPTAQGQKHLFSDEIECAVDEAVVLRQQLRKMTIRDIHDVESMGELNSLAEVRLKITRCIADAYIGEVLMAQGNGTDLEKALAHLSIQVRDVIQGNTEQLELIKQRALTALSFELSVGKASRRPFHWPLEFPEVFSLDGGFDCIVGNPPFLGGQKITGAMGDCYREYLVNTIACGKRGSADLVAYFFIKANALLCSSGFFGLIAVNTISEGNTREIGLDSILRCGSVIYRANPNLVWPGKANVVTSSVFVSKSNWNAPFYISGQQVPVISSALTQRENWQPVKLKDSCGLVFQGVITLGDGFLIDNDTYEKWVDENDESCKVVFDYLIGKEVNQSPRHLPARKVINFFDWSMDTSSKFTSAFNYVIDHVKENRENDKDKGAREAWWRFLRPRPELFHLLGRGGSFEKHPKGWDKDKKALDRVIVFATGATKYPCFTLVPNTYIYAHSLCVIASDSYSLFSCLSSDIHGIWAFEHGSRLHERLRYTHGDIFETFPLPGEKNHEILSNLGAEFFEERAGYMVNKNVGMTKFYNEFHNPAINSPSLSKLRELQVSINHAVLRAYGFDDIDLNHDFHEVAYLPEGKNIRYTICESAREKLLYRLASLNKIRYEEGQKGGINDL
ATGAGTTTAAATAACATTCACGCTGAATGGCTTTCTCTGGTAGATATTTCGGGACCTTTTCTCGCTATCCCAGTATTAACGCATACATTTCCTCAGGGACTAGAGGTGCTTAGTAGCTTCAAGCGTAGGCGGTTGCGCCAAGCCTATGATGAATGGCGGGAGGCATTAGAACTGGAAGATCCTCAGCTAGAAGAATTGCACTCTGCATGGATAGACGAAGTGCTGTCACGAGTGTTGGAATTTGATGATGATGGTAAAGGTGATGTGCTGAAACGTGAAGAGTGGTGTAGAAGCCACTTGAAATCAGTATTGCCAGATCAGGGAGTGGTTGAATATCCCGATTTGGCAGTTGTTGATGAACAGCAAGATAACAAGCCATTGTTACTCATACATACCTATAAACCAAATGTAGAACTAGAATCAACAGTAAAATATGAGGGATGGATAACCACCCCGGCAGATCGTATGGTTCAGTTATGTCGCTCACTTGGTTGCCGTCTCGGATTGCTAACCAACGGCGAATGCTGGATGTTAGTAGATGCACCAGTGGGTGCTGTAACGTCTTTCTCAAGCTGGTACGCACGTCTTTGGAGTCAAGAGCCAATCACACTGCAAGCATTCGTGCATTTACTAGGAATCCGCCGTTTCTTCGTTGACGAGTCTGAGCAGCTACCTGCATTGTTTGATGCTTCGCTGAAGTACCAGGATGAAGTGACTGACGCTTTAGGTGAACAAGTTTGCCGTGCTGTAGAAGTGTTAATTCAGTCGCTGGACAAAGCCGATCAGGATCGAAATCGTGAATTATTGCACGATGTCAATGAAACAGAGCTCTATGAAGCTGCGTTGGCAGTGATGATGCGATTGGTATTCCTGCTCTCAGCTGAGGAACAGGGCCTACTGCTCTTAGGTGACGAATGCTATGAGTCTAATTACGCAATTTCCACTTTGCGTATGCAACTACGGCAAGAACCTTCAGAGCTCCTTGAGCGACGTTGGGATGCCTGGACACGATTGCTTTCTACCTTCCGTGCAGTTTATGGCGGGGTGGAGCATGAAGAGCTGCGCCTACCGGCGTTAGGGAGTTCATTGTTTGATCCTGACCATTTTCCTTTTCTTGAAGGAAGAACTAAAGGCTCAAACTGGAAAATCGATACAGCTGTACCCTTACCAATAAATAATCGAACGGTTCTCCTATTACTGGAATCTATCCAACTATTTCAGGGGCGTACTCTTTCTTACCGCGCATTAGACGTTGAGCAGATCGGCTATGTCTATGAAGGTTTACTGGAACGTACTGTCAAACGTGCCGCTGAAGTGACGTTAGAACTGAATGCTACCAAAAAAGCTCAATCGCCTTGGGTTACGTTAGCTGAACTAGATTCAGTAAGTATGGACGGTACTGAGCAGCTGATTGAGCTACTGCATGAACGTTCTGGTAGTTCGATCAGTAAGATACGTAATGATCTATCCAAACTAATTGATGATAGTCTGATTGAACGCCTCTTGATTACCTGTCAGGGGGATACCAAATTACGTGATCGAATTAAACCTTATGTACATTTGCTTCGTACAGATCCTTGGGGCTATCCGCTGGTTTATCCAGCAGGATCTTTTATTGTAACTAGTGGTTCCAATCGTCGTGAAACAGGTACTCATTACACACCTAAGTCACTTACCGAGGCGATTGTTGCTGAAACACTAACACCACTGACTTATATTGGCCCAACAGAGGGTATTCCACGTGAAAAGTGGCAACTAAAATCCTCTTCTGAACTACTCACTCTCAAGATTTGTGATCCTGCAATGGGTTCGGGAGCCTTTTTAGTTCAAACTTGCCGCTGGCTGGCCGATCGACTTGTTGAATCTTGGACTTTAGCTGAAGAGTCAGGAAAAAAAATCAGTGTTGATGGCTACATTCTAGGTACTTCGAGTACTCAAGAATATCTACCACATGATACAGACGCTCGAATAGTTATTGCTCGTCGGTTAATTGCCGAACGCTGTCTTTACGGTGTTGACTTAAATCCATTGGCTGTTGAGCTAGCTAAACTTTCCATTTGGTTGGTAACACTCGCTAAAGATCGACCATTTGGTTTTCTTGAGCACAATTTACGGTGTGGTGACAGCTTACTTGGTATTGAACGAATAGATCAGCTTACCCAGTTTTCGATGAATCCTACAGCCCAAGGACAAAAGCATCTTTTTAGCGACGAAATTGAATGTGCGGTAGATGAAGCAGTTGTGCTACGTCAGCAGTTGCGCAAAATGACAATTCGCGACATACATGATGTGGAATCAATGGGAGAATTGAACTCGCTTGCAGAAGTTAGGCTGAAAATAACTAGATGTATTGCAGATGCGTATATCGGAGAAGTCCTTATGGCGCAAGGCAATGGTACGGATCTCGAAAAGGCTTTAGCTCATCTTTCCATTCAAGTTAGAGATGTTATACAAGGCAATACAGAACAACTTGAGTTGATAAAACAAAGAGCACTCACAGCTTTGTCTTTTGAACTATCCGTAGGAAAAGCTTCTCGGAGACCTTTCCATTGGCCGTTAGAATTTCCGGAGGTATTTAGTCTTGATGGTGGTTTTGATTGTATTGTCGGAAATCCACCATTCCTGGGCGGGCAAAAAATTACAGGTGCCATGGGCGATTGTTATCGCGAATATTTGGTAAATACAATCGCATGTGGTAAGCGAGGTTCTGCTGACTTAGTTGCGTACTTTTTTATTAAAGCCAATGCTTTACTTTGCTCTAGTGGTTTCTTTGGATTGATAGCGGTTAATACGATATCAGAAGGGAACACTAGAGAAATTGGACTTGATTCGATACTTCGTTGTGGTTCGGTGATATATAGGGCAAACCCAAACCTAGTATGGCCTGGAAAAGCAAATGTAGTCACAAGTAGTGTTTTTGTTTCTAAATCAAATTGGAATGCTCCTTTTTATATCTCAGGTCAACAAGTTCCTGTAATTAGTTCCGCGCTAACACAACGGGAAAACTGGCAACCCGTTAAGTTAAAAGACAGTTGTGGTCTTGTATTTCAAGGGGTTATTACGCTTGGTGATGGATTTTTGATAGATAACGATACCTATGAAAAATGGGTGGACGAAAATGATGAATCCTGCAAAGTAGTATTCGATTATTTGATTGGTAAAGAAGTCAATCAGTCACCAAGGCACCTGCCCGCACGGAAAGTGATCAATTTTTTTGACTGGAGTATGGATACTAGCTCTAAATTCACAAGTGCTTTTAACTATGTAATAGATCATGTTAAAGAAAATAGAGAAAATGATAAAGATAAAGGAGCAAGGGAGGCTTGGTGGCGATTTCTTCGGCCGCGGCCTGAACTTTTTCATCTTCTTGGACGAGGAGGTTCTTTTGAAAAACATCCGAAAGGATGGGATAAGGACAAAAAAGCACTTGACCGTGTAATTGTTTTTGCAACTGGCGCGACAAAATACCCCTGCTTCACGTTAGTCCCAAACACTTATATTTATGCACATTCTCTATGTGTAATTGCCAGTGACAGCTATTCATTATTTTCGTGTTTATCATCAGATATCCATGGAATATGGGCGTTCGAGCATGGTTCACGGTTGCATGAACGTCTTAGATATACCCATGGAGATATCTTTGAAACATTCCCTTTGCCAGGTGAGAAAAATCATGAAATATTATCAAATCTTGGGGCAGAGTTTTTCGAAGAAAGGGCGGGATATATGGTGAATAAAAATGTTGGTATGACCAAGTTTTACAACGAATTCCATAATCCGGCGATAAATTCACCATCACTCAGCAAATTACGTGAATTGCAGGTGAGTATAAACCATGCTGTGCTGCGTGCGTATGGTTTTGATGATATAGATTTAAATCATGATTTTCATGAGGTTGCATACCTTCCTGAAGGAAAGAATATTCGTTACACGATTTGTGAGTCTGCACGGGAAAAATTACTCTATCGGTTGGCCTCATTGAATAAAATTCGTTATGAAGAAGGGCAGAAAGGTGGGATTAACGATCTC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,039 total letters Query= IDP96868.102 Length=4434 Score E Sequences producing significant alignments: (Bits) Value 0_wt 7267 0.0 H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 110 ACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATG 199 0 255 ............. 243 A M S L N N I H A E W L S L V D I S G P F L A I P V L T H T Query_1 200 CCATGAGTTTAAATAACATTCACGCTGAATGGCTTTCTCTGGTAGATATTTCGGGACCTTTTCTCGCTATCCCAGTATTAACGCATACAT 289 0 1 ........................................................................................ 88 0 1950 ........... 1940 F P Q G L E V L S S F K R R R L R Q A Y D E W R E A L E L E Query_1 290 TTCCTCAGGGACTAGAGGTGCTTAGTAGCTTCAAGCGTAGGCGGTTGCGCCAAGCCTATGATGAATGGCGGGAGGCATTAGAACTGGAAG 379 0 89 .......................................................................................... 178 D P Q L E E L H S A W I D E V L S R V L E F D D D G K G D V Query_1 380 ATCCTCAGCTAGAAGAATTGCACTCTGCATGGATAGACGAAGTGCTGTCACGAGTGTTGGAATTTGATGATGATGGTAAAGGTGATGTGC 469 0 179 .......................................................................................... 268 L K R E E W C R S H L K S V L P D Q G V V E Y P D L A V V D Query_1 470 TGAAACGTGAAGAGTGGTGTAGAAGCCACTTGAAATCAGTATTGCCAGATCAGGGAGTGGTTGAATATCCCGATTTGGCAGTTGTTGATG 559 0 269 .......................................................................................... 358 E Q Q D N K P L L L I H T Y K P N V E L E S T V K Y E G W I Query_1 560 AACAGCAAGATAACAAGCCATTGTTACTCATACATACCTATAAACCAAATGTAGAACTAGAATCAACAGTAAAATATGAGGGATGGATAA 649 0 359 .......................................................................................... 448 T T P A D R M V Q L C R S L G C R L G L L T N G E C W M L V Query_1 650 CCACCCCGGCAGATCGTATGGTTCAGTTATGTCGCTCACTTGGTTGCCGTCTCGGATTGCTAACCAACGGCGAATGCTGGATGTTAGTAG 739 0 449 .......................................................................................... 538 D A P V G A V T S F S S W Y A R L W S Q E P I T L Q A F V H Query_1 740 ATGCACCAGTGGGTGCTGTAACGTCTTTCTCAAGCTGGTACGCACGTCTTTGGAGTCAAGAGCCAATCACACTGCAAGCATTCGTGCATT 829 0 539 .......................................................................................... 628 L L G I R R F F V D E S E Q L P A L F D A S L K Y Q D E V T Query_1 830 TACTAGGAATCCGCCGTTTCTTCGTTGACGAGTCTGAGCAGCTACCTGCATTGTTTGATGCTTCGCTGAAGTACCAGGATGAAGTGACTG 919 0 629 .......................................................................................... 718 0 1085 ...........C.. 1098 D A L G E Q V C R A V E V L I Q S L D K A D Q D R N R E L L Query_1 920 ACGCTTTAGGTGAACAAGTTTGCCGTGCTGTAGAAGTGTTAATTCAGTCGCTGGACAAAGCCGATCAGGATCGAAATCGTGAATTATTGC 1009 0 719 .......................................................................................... 808 H D V N E T E L Y E A A L A V M M R L V F L L S A E E Q G L Query_1 1010 ACGATGTCAATGAAACAGAGCTCTATGAAGCTGCGTTGGCAGTGATGATGCGATTGGTATTCCTGCTCTCAGCTGAGGAACAGGGCCTAC 1099 0 809 .......................................................................................... 898 L L L G D E C Y E S N Y A I S T L R M Q L R Q E P S E L L E Query_1 1100 TGCTCTTAGGTGACGAATGCTATGAGTCTAATTACGCAATTTCCACTTTGCGTATGCAACTACGGCAAGAACCTTCAGAGCTCCTTGAGC 1189 0 899 .......................................................................................... 988 R R W D A W T R L L S T F R A V Y G G V E H E E L R L P A L Query_1 1190 GACGTTGGGATGCCTGGACACGATTGCTTTCTACCTTCCGTGCAGTTTATGGCGGGGTGGAGCATGAAGAGCTGCGCCTACCGGCGTTAG 1279 0 989 .......................................................................................... 1078 G S S L F D P D H F P F L E G R T K G S N W K I D T A V P L Query_1 1280 GGAGTTCATTGTTTGATCCTGACCATTTTCCTTTTCTTGAAGGAAGAACTAAAGGCTCAAACTGGAAAATCGATACAGCTGTACCCTTAC 1369 0 1079 .......................................................................................... 1168 0 677 ...........G.. 690 0 3161 .... 3158 P I N N R T V L L L L E S I Q L F Q G R T L S Y R A L D V E Query_1 1370 CAAT-AAATAATCGAACGGTTCTCCTATTACTGGAATCTATCCAACTATTTCAGGGGCGTACTCTTTCTTACCGCGCATTAGACGTTGAG 1458 0 1169 ....-..................................................................................... 1257 0 3157 ....C........... 3142 Q I G Y V Y E G L L E R T V K R A A E V T L E L N A T K K A Query_1 1459 CAGATCGGCTATGTCTATGAAGGTTTACTGGAACGTACTGTCAAACGTGCCGCTGAAGTGACGTTAGAACTGAATGCTACCAAAAAAGCT 1548 0 1258 .......................................................................................... 1347 Q S P W V T L A E L D S V S M D G T E Q L I E L L H E R S G Query_1 1549 CAATCGCCTTGGGTTACGTTAGCTGAACTAGATTCAGTAAGTATGGACGGTACTGAGCAGCTGATTGAGCTACTGCATGAACGTTCTGGT 1638 0 1348 .......................................................................................... 1437 0 3605 ........... 3615 S S I S K I R N D L S K L I D D S L I E R L L I T C Q G D T Query_1 1639 AGTTCGATCAGTAAGATACGTAATGATCTATCCAAACTAATTGATGATAGTCTGATTGAACGCCTCTTGATTACCTGTCAGGGGGATACC 1728 0 1438 .......................................................................................... 1527 0 3804 . 3804 K L R D R I K P Y V H L L R T D P W G Y P L V Y P A G S F I Query_1 1729 AAATTACGTGATCGAATTAAACCTTATGTACATTTGCTTCGTACAGATCCTTGGGGCTATCCGCTGGTTTATCCAGCAGGATCTTTTATT 1818 0 1528 .......................................................................................... 1617 0 3805 ........... 3815 V T S G S N R R E T G T H Y T P K S L T E A I V A E T L T P Query_1 1819 GTAACTAGTGGTTCCAATCGTCGTGAAACAGGTACTCATTACACACCTAAGTCACTTACCGAGGCGATTGTTGCTGAAACACTAACACCA 1908 0 1618 .......................................................................................... 1707 L T Y I G P T E G I P R E K W Q L K S S S E L L T L K I C D Query_1 1909 CTGACTTATATTGGCCCAACAGAGGGTATTCCACGTGAAAAGTGGCAACTAAAATCCTCTTCTGAACTACTCACTCTCAAGATTTGTGAT 1998 0 1708 .......................................................................................... 1797 P A M G S G A F L V Q T C R W L A D R L V E S W T L A E E S Query_1 1999 CCTGCAATGGGTTCGGGAGCCTTTTTAGTTCAAACTTGCCGCTGGCTGGCCGATCGACTTGTTGAATCTTGGACTTTAGCTGAAGAGTCA 2088 0 1798 .......................................................................................... 1887 G K K I S V D G Y I L G T S S T Q E Y L P H D T D A R I V I Query_1 2089 GGaaaaaaaaTCAGTGTTGATGGCTACATTCTAGGTACTTCGAGTACTCAAGAATATCTACCACATGATACAGACGCTCGAATAGTTATT 2178 0 1888 .......................................................................................... 1977 0 48 ........... 38 A R R L I A E R C L Y G V D L N P L A V E L A K L S I W L V Query_1 2179 GCTCGTCGGTTAATTGCCGAACGCTGTCTTTACGGTGTTGACTTAAATCCATTGGCTGTTGAGCTAGCTAAACTTTCCATTTGGTTGGTA 2268 0 1978 .......................................................................................... 2067 T L A K D R P F G F L E H N L R C G D S L L G I E R I D Q L Query_1 2269 ACACTCGCTAAAGATCGACCATTTGGTTTTCTTGAGCACAATTTACGGTGTGGTGACAGCTTACTTGGTATTGAACGAATAGATCAGCTT 2358 0 2068 .......................................................................................... 2157 T Q F S M N P T A Q G Q K H L F S D E I E C A V D E A V V L Query_1 2359 ACCCAGTTTTCGATGAATCCTACAGCCCAAGGACAAAAGCATCTTTTTAGCGACGAAATTGAATGTGCGGTAGATGAAGCAGTTGTGCTA 2448 0 2158 .......................................................................................... 2247 R Q Q L R K M T I R D I H D V E S M G E L N S L A E V R L K Query_1 2449 CGTCAGCAGTTGCGCAAAATGACAATTCGCGACATACATGATGTGGAATCAATGGGAGAATTGAACTCGCTTGCAGAAGTTAGGCTGAAA 2538 0 2248 .......................................................................................... 2337 I T R C I A D A Y I G E V L M A Q G N G T D L E K A L A H L Query_1 2539 ATAACTAGATGTATTGCAGATGCGTATATCGGAGAAGTCCTTATGGCGCAAGGCAATGGTACGGATCTCGAAAAGGCTTTAGCTCATCTT 2628 0 2338 .......................................................................................... 2427 S I Q V R D V I Q G N T E Q L E L I K Q R A L T A L S F E L Query_1 2629 TCCATTCAAGTTAGAGATGTTATACAAGGCAATACAGAACAACTTGAGTTGATAAAACAAAGAGCACTCACAGCTTTGTCTTTTGAACTA 2718 0 2428 .......................................................................................... 2517 S V G K A S R R P F H W P L E F P E V F S L D G G F D C I V Query_1 2719 TCCGTAGGAAAAGCTTCTCGGAGACCTTTCCATTGGCCGTTAGAATTTCCGGAGGTATTTAGTCTTGATGGTGGTTTTGATTGTATTGTC 2808 0 2518 .......................................................................................... 2607 G N P P F L G G Q K I T G A M G D C Y R E Y L V N T I A C G Query_1 2809 GGAAATCCACCATTCCTGGGCGGGCAAAAAATTACAGGTGCCATGGGCGATTGTTATCGCGAATATTTGGTAAATACAATCGCATGTGGT 2898 0 2608 .......................................................................................... 2697 K R G S A D L V A Y F F I K A N A L L C S S G F F G L I A V Query_1 2899 AAGCGAGGTTCTGCTGACTTAGTTGCGTACTTTTTTATTAAAGCCAATGCTTTACTTTGCTCTAGTGGTTTCTTTGGATTGATAGCGGTT 2988 0 2698 .......................................................................................... 2787 N T I S E G N T R E I G L D S I L R C G S V I Y R A N P N L Query_1 2989 AATACGATATCAGAAGGGAACACTAGAGAAATTGGACTTGATTCGATACTTCGTTGTGGTTCGGTGATATATAGGGCAAACCCAAACCTA 3078 0 2788 .......................................................................................... 2877 V W P G K A N V V T S S V F V S K S N W N A P F Y I S G Q Q Query_1 3079 GTATGGCCTGGAAAAGCAAATGTAGTCACAAGTAGTGTTTTTGTTTCTAAATCAAATTGGAATGCTCCTTTTTATATCTCAGGTCAACAA 3168 0 2878 .......................................................................................... 2967 V P V I S S A L T Q R E N W Q P V K L K D S C G L V F Q G V Query_1 3169 GTTCCTGTAATTAGTTCCGCGCTAACACAACGGGAAAACTGGCAACCCGTTAAGTTAAAAGACAGTTGTGGTCTTGTATTTCAAGGGGTT 3258 0 2968 .......................................................................................... 3057 I T L G D G F L I D N D T Y E K W V D E N D E S C K V V F D Query_1 3259 ATTACGCTTGGTGATGGATTTTTGATAGATAACGATACCTATGAAAAATGGGTGGACGAAAATGATGAATCCTGCAAAGTAGTATTCGAT 3348 0 3058 .......................................................................................... 3147 0 1183 ...... 1178 Y L I G K E V N Q S P R H L P A R K V I N F F D W S M D T S Query_1 3349 TATTTGATTGGTAAAGAAGTCAATCAGTCACCAAGGCACCTGCCCGCACGGAAAGTGATCAAtttttttGACTGGAGTATGGATACTAGC 3438 0 3148 .......................................................................................... 3237 0 1177 .....-........ 1165 S K F T S A F N Y V I D H V K E N R E N D K D K G A R E A W Query_1 3439 TCTAAATTCACAAGTGCTTTTAACTATGTAATAGATCATGTTAAAGAAAATAGAGAAAATGATAAAGATAAAGGAGCAAGGGAGGCTTGG 3528 0 3238 .......................................................................................... 3327 W R F L R P R P E L F H L L G R G G S F E K H P K G W D K D Query_1 3529 TGGCGATTTCTTCGGCCGCGGCCTGAACTTTTTCATCTTCTTGGACGAGGAGGTTCTTTTGAAAAACATCCGAAAGGATGGGATAAGGAC 3618 0 3328 .......................................................................................... 3417 K K A L D R V I V F A T G A T K Y P C F T L V P N T Y I Y A Query_1 3619 AAAAAAGCACTTGACCGTGTAATTGTTTTTGCAACTGGCGCGACAAAATACCCCTGCTTCACGTTAGTCCCAAACACTTATATTTATGCA 3708 0 3418 .......................................................................................... 3507 H S L C V I A S D S Y S L F S C L S S D I H G I W A F E H G Query_1 3709 CATTCTCTATGTGTAATTGCCAGTGACAGCTATTCATTATTTTCGTGTTTATCATCAGATATCCATGGAATATGGGCGTTCGAGCATGGT 3798 0 3508 .......................................................................................... 3597 S R L H E R L R Y T H G D I F E T F P L P G E K N H E I L S Query_1 3799 TCACGGTTGCATGAACGTCTTAGATATACCCATGGAGATATCTTTGAAACATTCCCTTTGCCAGGTGAGAAAAATCATGAAATATTATCA 3888 0 3598 .......................................................................................... 3687 0 1421 ........... 1431 N L G A E F F E E R A G Y M V N K N V G M T K F Y N E F H N Query_1 3889 AATCTTGGGGCAGAGTTTTTCGAAGAAAGGGCGGGATATATGGTGAATAAAAATGTTGGTATGACCAAGTTTTACAACGAATTCCATAAT 3978 0 3688 .......................................................................................... 3777 P A I N S P S L S K L R E L Q V S I N H A V L R A Y G F D D Query_1 3979 CCGGCGATAAATTCACCATCACTCAGCAAATTACGTGAATTGCAGGTGAGTATAAACCATGCTGTGCTGCGTGCGTATGGTTTTGATGAT 4068 0 3778 .......................................................................................... 3867 0 1527 ............ 1538 I D L N H D F H E V A Y L P E G K N I R Y T I C E S A R E K Query_1 4069 ATAGATTTAAATCATGATTTTCATGAGGTTGCATACCTTCCTGAAGGAAAGAATATTCGTTACACGATTTGTGAGTCTGCACGGGAAAAA 4158 0 3868 .......................................................................................... 3957 0 3887 ............ 3876 L L Y R L A S L N K I R Y E E G Q K G G I N D L * H W K W I Query_1 4159 TTACTCTATCGGTTGGCCTCATTGAATAAAATTCGTTATGAAGAAGGGCAGAAAGGTGGGATTAACGATCTCTAACATTGGAAGTGGATA 4248 0 3958 ........................................................................ 4029 T D P N S S S V D K L A A A L E H H H H H H * D P A A N K A Query_1 4249 ACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCC 4338 R K E A E L A A A T A E Q * L A * P L Query_1 4339 CGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTG 4396 0 1987 ....AC............ 1970 0 3059 ........... 3049 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 17597760 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 4,039 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96869.103 (IDP96869.1-425.F..pMCSG73)
MNNDDYPWFRKRGYLHFDEPVSLKKAVKYVSSPEKIIKHSFLPFLSFEVKSFKIKKDKSTKQLSKTEKLRPIAYSSHLDSHIYAFYAEYLTGHYELLIQENNLHENILAFRSLNKSNIEFAKRAFDTITEMGECSAVALDLSGFFDNLDHQILKHQWCKVIGTEALPQDHFAIYKSITRYSKVDKNRAYEILGISKNNPKYNRRKICTPVDFRNKIRKNGLIIVNNSQKGIPQGSPISALLSNIYMLDFDIEMRDYAQERGGHYYRYCDDMLFIVPTKYNKTLAGDVAQRIKHLKVELNTKKTEIRDFIYKDSTLVANMPLQYLGFIFDGSNILLRSSSLARYSERMKRGVRLAKATMDSKNRIRENKGEALKALFKKKLYARYSHIGRRNFLTYGYRAAKIMNSKAIKRQLKPLQKRLENEILK
ATGAATAATGATGATTACCCATGGTTCAGAAAACGTGGTTATTTGCATTTCGATGAACCTGTTTCATTAAAAAAAGCGGTTAAATATGTTTCCTCTCCAGAAAAAATAATAAAACATTCTTTTCTGCCATTTTTAAGCTTTGAAGTAAAATCGTTTAAAATCAAAAAAGACAAATCAACAAAACAATTAAGTAAAACTGAAAAATTAAGACCTATTGCCTATTCCTCACATTTGGATAGTCATATTTATGCATTTTACGCAGAATATCTTACTGGACATTATGAATTATTGATCCAAGAAAACAATTTACACGAGAACATCCTTGCCTTCAGATCTTTAAATAAAAGCAATATAGAATTTGCCAAGAGAGCATTTGATACAATTACTGAAATGGGTGAGTGTAGCGCTGTTGCATTAGATCTTTCTGGTTTTTTTGACAATTTAGATCATCAAATTTTGAAACACCAGTGGTGCAAAGTTATTGGGACTGAAGCGTTGCCGCAAGACCATTTTGCCATATACAAAAGTATAACAAGATATTCTAAAGTTGATAAAAATAGAGCGTATGAGATTTTAGGTATATCAAAGAATAACCCCAAGTATAATAGACGCAAGATCTGCACCCCTGTTGATTTTAGAAATAAGATTAGAAAAAATGGTCTTATTATAGTTAATAATTCCCAAAAAGGTATACCCCAAGGCTCGCCAATTAGTGCTCTACTTTCAAATATATATATGCTTGACTTTGATATTGAAATGAGAGATTACGCGCAGGAACGTGGTGGCCATTATTATCGCTATTGTGATGATATGCTATTCATTGTACCAACTAAGTATAATAAAACTCTAGCAGGTGATGTAGCCCAGCGGATTAAGCATCTTAAGGTAGAACTCAATACTAAGAAAACTGAGATTCGAGATTTTATATACAAAGACAGTACCTTAGTGGCAAATATGCCTTTACAGTATCTTGGGTTTATTTTTGATGGGAGTAATATATTATTACGTTCATCTTCTCTCGCAAGATATTCGGAACGAATGAAAAGAGGTGTCCGCTTAGCAAAAGCTACAATGGACAGCAAGAATAGGATTAGAGAAAATAAAGGTGAAGCTTTAAAAGCTTTATTTAAGAAAAAATTATATGCCAGATATTCACATATTGGAAGAAGGAATTTTTTGACTTATGGTTATCGCGCCGCGAAGATCATGAATTCGAAAGCTATAAAAAGACAGTTAAAACCATTGCAGAAAAGATTGGAAAATGAAATACTAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,731 total letters Query= IDP96869.103 Length=1680 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2300 0.0 1_04C_C4_IDP96869.103_v73F.seq 1867 0.0 2_10C_C4_IDP96869.103_T7_Term.seq 1847 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 1 45 ...........A........G....------....................... 92 1 27 .....C........... 43 A M N N D D Y P W F R K R G Y L H F D E P V S L K K A V K Y Query_1 199 GCCATGAATAATGATGATTACCCATGGTTCAGAAAACGTGGTTATTTGCATTTCGATGAACCTGTTTCATTaaaaaaaGCGGTTAAATAT 288 0 1 ....................................................................................... 87 1 93 .......................................................................................... 182 V S S P E K I I K H S F L P F L S F E V K S F K I K K D K S Query_1 289 GTTTCCTCTCCAGAAAAAATAATAAAACATTCTTTTCTGCCATTTTTAAGCTTTGAAGtaaaatcgtttaaaatcaaaaaagacaaatca 378 0 88 .......................................................................................... 177 1 183 .......................................................................................... 272 T K Q L S K T E K L R P I A Y S S H L D S H I Y A F Y A E Query_1 379 acaaaacaattaagtaaaactgaaaaatTAAGACCTATTGCCTATTCCTCAC-ATTTGG-ATAGTCATATTT-ATGCATTTTACGCAGAA 465 0 178 ....................................................-......-............-................. 264 1 273 ....................................................-......-............-................. 359 2 1144 ...N..G....N..N-....N...............C......G............N....N..--........ 1074 Y L T G H Y E L L I Q E N N L H E N I L A F R S L N K S N Query_1 466 TAT-CTTACTGGACATTATGAATTATTGATCCAAGAAAACAATTTACACGAGAACATCCTTGCCTTCAGATCTTTAAATAAAA-GCAATA 553 0 265 ...-...............................................................................-...... 352 1 360 ...-...............................................................................-...... 447 2 1073 ...T.........................N.-......-...........GAG.......-.............N........A...... 987 I E F A K R A F D T I T E M G E C S A V A L D L S G F F D N Query_1 554 TAGAATTTGCCAAGAGAGCATTTGATACAATTACTGAAA-TGGGTGAGTGTAGCGCTGTTGCATTAGATCTTTCTGGtttttttGACAAT 642 0 353 .......................................-.................................................. 441 1 448 .......................................-.................................................. 536 2 986 ....-..................................A.................................................. 898 L D H Q I L K H Q W C K V I G T E A L P Q D H F A I Y K S I Query_1 643 TTAGATCATCAAATTTTGAAACACCAGTGGTGCAAAGTTATTGGGACTGAAGCGTTGCCGCAAGACCATTTTGCCATATACAAAAGTATA 732 0 442 .......................................................................................... 531 0 663 ........... 653 1 537 .......................................................................................... 626 1 758 ........... 748 2 897 .......................................................................................... 808 2 676 ........... 686 T R Y S K V D K N R A Y E I L G I S K N N P K Y N R R K I C Query_1 733 ACAAGATATTCTAAAGTTGATAAAAATAGAGCGTATGAGATTTTAGGTATATCAAAGAATAACCCCAAGTATAATAGACGCAAGATCTGC 822 0 532 .......................................................................................... 621 1 627 .......................................................................................... 716 2 807 .......................................................................................... 718 T P V D F R N K I R K N G L I I V N N S Q K G I P Q G S P I Query_1 823 ACCCCTGTTGATTTTAGAAATAAGATTAGAAAAAATGGTCTTATTATAGTTAATAATTCCCAAAAAGGTATACCCCAAGGCTCGCCAATT 912 0 622 .......................................................................................... 711 0 513 ........... 503 1 717 .......................................................................................... 806 1 608 ........... 598 2 717 .......................................................................................... 628 2 826 ........... 836 S A L L S N I Y M L D F D I E M R D Y A Q E R G G H Y Y R Query_1 913 AGTGCTCTAC-TTTCAAATATATATATGCTTGACTTTGATATTGAAATGAGAGATTACGCGCAGGAACGTGGTGGCCATTATTATCGCTA 1001 0 712 ..........-............................................................................... 800 1 807 ..........T............................................................................... 896 2 627 ..........-............................................................................... 539 Y C D D M L F I V P T K Y N K T L A G D V A Q R I K H L K V Query_1 1002 TTGTGATGATATGCTATTCATTGTACCAACTAAGTATAATAAAACTCTAGCAGGTGATGTAGCCCAGCGGATTAAGCATCTTAAGGTAGA 1091 0 801 .......................................................................................... 890 1 897 .......................................................................................... 986 2 538 .......................................................................................... 449 E L N T K K T E I R D F I Y K D S T L V A N M P L Q Y L G F Query_1 1092 ACTCAATACTAAGAAAACTGAGATTCGAGATTTTATATACAAAGACAGTACCTTAGTGGCAAATATGCCTTTACAGTATCTTGGGTTTAT 1181 0 891 .......................................................................................... 980 1 987 ........................................N.................N.......TN............-.N....... 1075 2 448 .......................................................................................... 359 I F D G S N I L L R S S S L A R Y S E R M K R G V R L A K A Query_1 1182 TTTTGATGGGAGTAATATATTATTACGTTCA-TCTTCTCTCGCAAGATATTCGGAACGAATGAAAAGAGGTGTCCGCTTAGCAAAAGCTA 1270 0 981 ...............................-.......................................................... 1069 1 1076 .................-NN...........T...........-...-N....--...-...-.NNN....N............ 1152 2 358 ...............................-.......................................................... 270 T M D S K N R I R E N K G E A L K A L F K K K L Y A R Y S H Query_1 1271 CAATGGACAGCAAGAATAGGATTAGAGAAAATAAAGGTGAAGCTTTAAAAGCTTTATTTAAGAAAAAATTATATGCCAGATATTCACATA 1360 0 1070 .......................................................................................... 1159 2 269 .......................................................................................... 180 I G R R N F L T Y G Y R A A K I M N S K A I K R Q L K P L Q Query_1 1361 TTGGAAGAAGGAATTTTTTGACTTATGGTTATCGCGCCGCGAAGATCATGAATTCGAAAGCTATAAAAAGACAGTTAAAACCATTGCAGA 1450 0 1160 .......................................................................................... 1249 2 179 .......................................................................................... 90 K R L E N E I L K * H W K W I T D P N S S S V D K L A A A L Query_1 1451 AAAGATTGGAAAATGAAATACTAAAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCG 1540 0 1250 .......................... 1275 2 89 ..............-...............................--..... 40 E H H H H H H * D P A A N K A R K E Query_1 1541 AGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCC--GAAAGGAA 1593 2 44 .....................CGA....... 14 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 6128512 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 3,731 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96870.104 (IDP96870.1-220.F..pMCSG73)
MSESVSYFDKVISLINAVVPVLAIYFAQRLWHAKNIERAHQAANDFLDEMTSLPMRVMLLETEMVRGLVRAESVISYKNKNEFVCELLRLMDNSNKIRLSFFNSYERVVRRGINIKPSQKHMKLEKSVIEFTEHVSKILQNAAEAISRSTTTEEYREPFRTLAGARIVITKNKNTLNEACAATKDISFDDYFSFPSAYGWFRHKWDSLIRPFLFKPFKNM
ATGAGTGAAAGTGTAAGTTACTTTGACAAAGTCATATCTTTAATAAATGCAGTGGTACCAGTCCTAGCTATTTATTTTGCTCAGAGGCTTTGGCATGCCAAAAATATTGAGCGAGCGCATCAGGCGGCAAATGATTTTCTTGACGAAATGACATCATTACCAATGAGAGTAATGTTACTAGAAACAGAGATGGTCAGAGGGTTAGTTAGAGCTGAATCAGTTATTAGTTATAAAAATAAAAATGAATTTGTGTGTGAGTTGCTGCGTCTTATGGATAATTCGAATAAGATCAGACTTTCGTTTTTTAATTCCTATGAAAGAGTTGTTCGAAGGGGGATTAACATCAAACCTTCCCAAAAACATATGAAACTCGAAAAATCTGTTATTGAGTTTACTGAACATGTTTCAAAAATTCTCCAGAATGCAGCAGAGGCTATTAGTAGATCGACAACAACAGAAGAATATAGAGAGCCATTCAGAACGTTAGCTGGTGCTAGAATTGTTATAACCAAAAATAAAAATACACTCAATGAAGCCTGTGCTGCTACAAAGGATATTTCGTTTGATGACTATTTTTCCTTTCCTTCAGCATATGGTTGGTTCAGGCATAAATGGGATAGTTTGATTCGACCTTTTTTATTTAAACCGTTTAAAAATATG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,111 total letters Query= IDP96870.104 Length=1065 Score E Sequences producing significant alignments: (Bits) Value 2_10D_D4_IDP96870.104_T7_Term.seq 1296 0.0 1_04D_D4_IDP96870.104_v73F.seq 1296 0.0 0_wt 1191 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 2 776 ...........A........G....------....................... 729 2 794 .....C........... 778 1 43 ...........A........G....------....................... 90 1 27 ...C........... 41 A M S E S V S Y F D K V I S L I N A V V P V L A I Y F A Q R Query_1 199 GCCATGAGTGAAAGTGTAAGTTACTTTGACAAAGTCATATCTTTAATAAATGCAGTGGTACCAGTCCTAGCTATTTATTTTGCTCAGAGG 288 2 728 .......................................................................................... 639 1 91 .......................................................................................... 180 0 1 ....................................................................................... 87 L W H A K N I E R A H Q A A N D F L D E M T S L P M R V M L Query_1 289 CTTTGGCATGCCAAAAATATTGAGCGAGCGCATCAGGCGGCAAATGATTTTCTTGACGAAATGACATCATTACCAATGAGAGTAATGTTA 378 2 638 .......................................................................................... 549 2 624 .............. 637 1 181 .......................................................................................... 270 1 195 .............. 182 0 88 .......................................................................................... 177 0 102 .............. 89 L E T E M V R G L V R A E S V I S Y K N K N E F V C E L L R Query_1 379 CTAGAAACAGAGATGGTCAGAGGGTTAGTTAGAGCTGAATCAGTTATTAGTTATAAAAATAAAAATGAATTTGTGTGTGAGTTGCTGCGT 468 2 548 .......................................................................................... 459 2 215 ............ 204 1 271 .......................................................................................... 360 1 604 ............ 615 0 178 .......................................................................................... 267 0 511 ............ 522 L M D N S N K I R L S F F N S Y E R V V R R G I N I K P S Q Query_1 469 CTTATGGATAATTCGAATAAGATCAGACTTTCGTTTTTTAATTCCTATGAAAGAGTTGTTCGAAGGGGGATTAACATCAAACCTTCCCAA 558 2 458 .......................................................................................... 369 1 361 .......................................................................................... 450 0 268 .......................................................................................... 357 K H M K L E K S V I E F T E H V S K I L Q N A A E A I S R S Query_1 559 AAACATATGAAACTCGAAAAATCTGTTATTGAGTTTACTGAACATGTTTCAAAAATTCTCCAGAATGCAGCAGAGGCTATTAGTAGATCG 648 2 368 .......................................................................................... 279 1 451 .......................................................................................... 540 0 358 .......................................................................................... 447 T T T E E Y R E P F R T L A G A R I V I T K N K N T L N E A Query_1 649 ACAACAACAGAAGAATATAGAGAGCCATTCAGAACGTTAGCTGGTGCTAGAATTGTTATAACCAAAAATAAAAATACACTCAATGAAGCC 738 2 278 .......................................................................................... 189 2 494 ............ 483 1 541 .......................................................................................... 630 1 325 ............ 336 0 448 .......................................................................................... 537 0 232 ............ 243 C A A T K D I S F D D Y F S F P S A Y G W F R H K W D S L I Query_1 739 TGTGCTGCTACAAAGGATATTTCGTTTGATGACTATTTTTCCTTTCCTTCAGCATATGGTTGGTTCAGGCATAAATGGGATAGTTTGATT 828 2 188 .......................................................................................... 99 1 631 .......................................................................................... 720 0 538 .......................................................................................... 627 R P F L F K P F K N M * H W K W I T D P N S S S V D K L A A Query_1 829 CGACCTTTTTTATTTAAACCGTTTAAAAATATGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCC 918 2 98 .....................................................--..... 41 1 721 .....................................................--..... 778 0 628 ................................. 660 A L E H H H H H H * D P A A N K A R K E A E L A A A T A E Q Query_1 919 GCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAA 1008 2 45 .....................N...A... 17 1 774 .........................................................-. 831 * L A * P L G A S K R V L R G F L L Query_1 1009 TAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAA 1064 1 832 ........................................-............... 886 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3213087 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 3,111 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96871.103 (IDP96871.1-311.F..pMCSG73)
MAQHHWPTWGNENVVNLLKSQRDLYRYINDQTLRMANEGLTRDEIAANFKLPDSLANTWANRGYYGSVSHDVKATYVLYLGWFDGNPATLDELPPEEAAKKFVDYMGGADAILKKAKEDYDQGNYRWVAQVVSKVVFADPNNQQARNLEADALEQLGYQAESGPWRNFYLTGAQELRNGVVKGPTPNTASPDTVRAMTPEMFFDYLAVHINGEKAGNAKSVFNIDLGSDGGQYKLELENGVLNHTANAQAKEADATLTLDRATLNKIILKEETLKQAEDKGEVKISGNGAKLDEMLGYMDPFDFWFNIVTP
ATGGCCCAGCATCACTGGCCGACCTGGGGCAACGAGAACGTGGTGAACCTGCTGAAGAGCCAGCGTGACCTGTATCGTTATATCAACGACCAGACCCTGAGGATGGCTAACGAAGGCCTGACCCGCGATGAAATCGCCGCTAACTTTAAGCTACCGGACAGCCTCGCCAATACCTGGGCTAACCGCGGCTACTACGGCTCGGTCAGCCACGATGTGAAGGCCACCTACGTGCTGTATCTCGGCTGGTTCGACGGCAACCCGGCCACCCTTGACGAACTGCCGCCGGAAGAAGCGGCGAAGAAGTTCGTCGATTACATGGGTGGCGCGGATGCCATCCTGAAGAAAGCGAAAGAGGACTATGACCAGGGTAACTACCGCTGGGTTGCCCAGGTGGTGAGCAAAGTCGTCTTCGCCGACCCTAACAACCAGCAGGCGCGTAATCTGGAAGCCGATGCCCTGGAGCAGTTAGGCTATCAGGCGGAGTCCGGCCCGTGGCGTAACTTCTATCTCACCGGCGCCCAGGAGCTGCGTAACGGTGTGGTAAAAGGCCCGACCCCGAACACCGCCAGCCCGGACACCGTCCGCGCCATGACCCCGGAAATGTTCTTTGATTATCTGGCGGTCCATATCAATGGCGAAAAAGCGGGTAACGCGAAGTCGGTGTTCAATATCGATCTCGGCAGCGATGGCGGCCAATATAAGCTGGAGCTGGAAAACGGCGTGCTGAACCACACCGCGAATGCGCAAGCCAAAGAGGCGGATGCCACCCTGACGCTGGATCGCGCCACCCTGAACAAAATCATCCTCAAAGAAGAGACCCTGAAGCAGGCGGAAGATAAGGGTGAGGTGAAAATCAGCGGTAACGGGGCTAAACTCGATGAAATGCTTGGCTATATGGACCCGTTTGACTTCTGGTTTAACATCGTGACGCCG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,404 total letters Query= IDP96871.103 Length=1338 Score E Sequences producing significant alignments: (Bits) Value 2_10E_E4_IDP96871.103_T7_Term.seq 1770 0.0 1_04E_E4_IDP96871.103_v73F.seq 1759 0.0 0_wt 1683 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGC-AGTTCGAAAAGGGTACCGAGAACCTGTACTT-CCAATCCA 196 2 1051 ...........A.C.......G....AG-----............T........ 1003 2 1067 ...C........... 1053 2 1066 .............. 1053 1 42 ...........A.-.......G....------.............-........ 87 1 24 .....C........... 40 N A M A Q H H W P T W G N E N V V N L L K S Q R D L Y R Y I Query_1 197 ATGCCATGGCCCAGCATCACTGGCCGACCTGGGGCAACGAGAACGTGGTGAACCTGCTGAAGAGCCAGCGTGACCTGTATCGTTATATCA 286 2 1002 .......................................................................................... 913 1 88 .......................................................................................... 177 0 1 ..................................................................................... 85 N D Q T L R M A N E G L T R D E I A A N F K L P D S L A N T Query_1 287 ACGACCAGACCCTGAGGATGGCTAACGAAGGCCTGACCCGCGATGAAATCGCCGCTAACTTTAAGCTACCGGACAGCCTCGCCAATACCT 376 2 912 .......................................................................................... 823 1 178 .......................................................................................... 267 0 86 .......................................................................................... 175 W A N R G Y Y G S V S H D V K A T Y V L Y L G W F D G N P A Query_1 377 GGGCTAACCGCGGCTACTACGGCTCGGTCAGCCACGATGTGAAGGCCACCTACGTGCTGTATCTCGGCTGGTTCGACGGCAACCCGGCCA 466 2 822 .......................................................................................... 733 1 268 .......................................................................................... 357 0 176 .......................................................................................... 265 T L D E L P P E E A A K K F V D Y M G G A D A I L K K A K E Query_1 467 CCCTTGACGAACTGCCGCCGGAAGAAGCGGCGAAGAAGTTCGTCGATTACATGGGTGGCGCGGATGCCATCCTGAAGAAAGCGAAAGAGG 556 2 732 .......................................................................................... 643 1 358 .......................................................................................... 447 0 266 .......................................................................................... 355 D Y D Q G N Y R W V A Q V V S K V V F A D P N N Q Q A R N L Query_1 557 ACTATGACCAGGGTAACTACCGCTGGGTTGCCCAGGTGGTGAGCAAAGTCGTCTTCGCCGACCCTAACAACCAGCAGGCGCGTAATCTGG 646 2 642 .......................................................................................... 553 1 448 .......................................................................................... 537 0 356 .......................................................................................... 445 E A D A L E Q L G Y Q A E S G P W R N F Y L T G A Q E L R N Query_1 647 AAGCCGATGCCCTGGAGCAGTTAGGCTATCAGGCGGAGTCCGGCCCGTGGCGTAACTTCTATCTCACCGGCGCCCAGGAGCTGCGTAACG 736 2 552 .......................................................................................... 463 1 538 .......................................................................................... 627 0 446 .......................................................................................... 535 G V V K G P T P N T A S P D T V R A M T P E M F F D Y L A V Query_1 737 GTGTGGTAAAAGGCCCGACCCCGAACACCGCCAGCCCGGACACCGTCCGCGCCATGACCCCGGAAATGTTCTTTGATTATCTGGCGGTCC 826 2 462 .......................................................................................... 373 1 628 .......................................................................................... 717 0 536 .......................................................................................... 625 H I N G E K A G N A K S V F N I D L G S D G G Q Y K L E L E Query_1 827 ATATCAATGGCGAAAAAGCGGGTAACGCGAAGTCGGTGTTCAATATCGATCTCGGCAGCGATGGCGGCCAATATAAGCTGGAGCTGGAAA 916 2 372 .......................................................................................... 283 1 718 .......................................................................................... 807 0 626 .......................................................................................... 715 N G V L N H T A N A Q A K E A D A T L T L D R A T L N K I I Query_1 917 ACGGCGTGCTGAACCACACCGCGAATGCGCAAGCCAAAGAGGCGGATGCCACCCTGACGCTGGATCGCGCCACCCTGAACAAAATCATCC 1006 2 282 .......................................................................................... 193 1 808 .......................................................................................... 897 0 716 .......................................................................................... 805 L K E E T L K Q A E D K G E V K I S G N G A K L D E M L G Y Query_1 1007 TCAAAGAAGAGACCCTGAAGCAGGCGGAAGATAAGGGTGAGGTGAAAATCAGCGGTAACGGGGCTAAACTCGATGAAATGCTTGGCTATA 1096 2 192 .......................................................................................... 103 1 898 .......................................................................................... 987 0 806 .......................................................................................... 895 M D P F D F W F N I V T P * H W K W I T D P N S S S V D K L Query_1 1097 TGGACCCGTTTGACTTCTGGTTTAACATCGTGACGCCGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTG 1186 2 102 ..........................................................--..... 40 1 988 ....................-.............NN..N.............. 1039 0 896 ...................................... 933 A A A L E H H H H H H * D P A A N K A R K E A E L A A A T A Query_1 1187 CGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTG 1276 2 44 ........................... 18 1 1049 ..........C...-...........-.........N-.............. 1097 E Q * L A * P L G A S K R V L R G F L L Query_1 1277 AGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAA 1337 1 1098 ....--T.........--...NN...........--C....-.....----N......... 1147 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4436632 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 3,404 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96873.103 (IDP96873.19-125.P(125)..pMCSG73)
TTIGGNEDKLTPAYLKQHLIVGKTTKAEVRQYFGAPDSGHTTVTASGKESWYYSVDKGINLVSLAGSMIPVTGASQAADAVDKSSQKDSNSLMIFFDEKGVVENWVR
ACAACGATTGGCGGTAATGAAGATAAATTAACGCCAGCCTATCTGAAACAACATCTCATTGTCGGAAAAACCACCAAGGCCGAGGTTCGTCAGTATTTTGGTGCCCCGGATTCTGGTCATACGACGGTGACGGCGTCGGGTAAAGAGAGCTGGTATTATTCAGTAGATAAAGGGATCAACCTGGTCAGCCTCGCGGGTTCCATGATCCCGGTAACCGGGGCATCGCAAGCTGCCGATGCGGTCGATAAGTCCAGCCAAAAAGATTCGAACTCGCTGATGATTTTCTTCGATGAAAAAGGCGTGGTGGAAAACTGGGTGCGT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,817 total letters Query= IDP96873.103 Length=726 Score E Sequences producing significant alignments: (Bits) Value 2_09H_H3_IDP96873.103_T7_Term.seq 684 0.0 1_03H_H3_IDP96873.103_v73F.seq 684 0.0 0_wt 580 4e-169 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 2 438 ...........A........G....------....................... 391 2 456 .....C........... 440 1 32 ...........A........G....------....................... 79 1 19 ............ 30 1 20 ........... 30 A T T I G G N E D K L T P A Y L K Q H L I V G K T T K A E V Query_1 199 GCCACAACGATTGGCGGTAATGAAGATAAATTAACGCCAGCCTATCTGAAACAACATCTCATTGTCGGAAAAACCACCAAGGCCGAGGTT 288 2 390 .......................................................................................... 301 1 80 .......................................................................................... 169 0 1 ....................................................................................... 87 R Q Y F G A P D S G H T T V T A S G K E S W Y Y S V D K G I Query_1 289 CGTCAGTATTTTGGTGCCCCGGATTCTGGTCATACGACGGTGACGGCGTCGGGTAAAGAGAGCTGGTATTATTCAGTAGATAAAGGGATC 378 2 300 .......................................................................................... 211 1 170 .......................................................................................... 259 0 88 .......................................................................................... 177 N L V S L A G S M I P V T G A S Q A A D A V D K S S Q K D S Query_1 379 AACCTGGTCAGCCTCGCGGGTTCCATGATCCCGGTAACCGGGGCATCGCAAGCTGCCGATGCGGTCGATAAGTCCAGCCAAAAAGATTCG 468 2 210 .......................................................................................... 121 1 260 .......................................................................................... 349 0 178 .......................................................................................... 267 N S L M I F F D E K G V V E N W V R * H W K W I T D P N S S Query_1 469 AACTCGCTGATGATTTTCTTCGATGAAAAAGGCGTGGTGGAAAACTGGGTGCGTTAACATTGGAAGTGGATAACGGATCCGAATTCGAGC 558 2 120 ..........................................................................--..... 42 1 350 ..........................................................................--..... 428 0 268 ...................................................... 321 S V D K L A A A L E H H H H H H * D P A A N K A R K E A E Query_1 559 TCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCC--GAAAGGAAGCTGAGT 646 2 46 .....................CGA........ 15 1 424 .....................--............... 459 L A A A T A E Q * L A * P L G A S K R V L R G F L L K Query_1 647 TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAA 726 1 460 ................................................................................ 539 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1970892 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 2,817 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96874.102 (IDP96874.1-156.F..pMCSG68)
MMTLRTFPMLHDLSDSLFADRFNRIDRLFSQLTGSTPLPSTPSYNIRRMGDNRYELTLSVPGWKESELEIETVGGQLNISGKREEEKTENGEEGWIHRGISRSDFRASYSLPEHVKVTGASLENGLLAIELHQDIPEEEKPQRIAINNNPAIEHKP
ATGATGACTCTCAGAACTTTTCCCATGTTGCACGATCTCTCCGACTCTCTGTTTGCGGACCGTTTTAACCGCATCGATCGCCTTTTTAGCCAGCTAACCGGCAGTACACCGTTGCCCTCCACGCCTTCTTACAATATCCGGCGGATGGGTGATAACCGCTATGAACTGACGCTCAGTGTGCCGGGCTGGAAAGAGAGCGAACTGGAAATTGAAACCGTCGGCGGTCAGCTGAACATTAGCGGTAAACGTGAAGAGGAGAAAACCGAAAACGGCGAAGAAGGATGGATCCATCGGGGTATCAGCCGCAGCGACTTCCGGGCCAGCTATAGTCTGCCGGAGCATGTGAAAGTGACCGGCGCTTCGCTGGAAAACGGCTTGCTGGCGATTGAATTACACCAGGATATCCCGGAAGAAGAGAAACCGCAGCGCATCGCCATCAACAACAACCCGGCGATTGAACACAAGCCG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,818 total letters Query= IDP96874.102 Length=873 Score E Sequences producing significant alignments: (Bits) Value 1_01E_E1_IDP96874.102_T7S.seq 1463 0.0 2_07E_E1_IDP96874.102_T7term.seq 1342 0.0 0_wt 845 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATT-CCCCTCTAGAAATAATTTTGTTTAACTTTA 89 1 2 .....N...........-.................. 36 2 761 ............................N..............................-............................-. 674 2 694 ...G...........T.T.C.C.C......-.....A... 732 * E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E Query_1 90 AGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAA 179 1 37 .......................................................................................... 126 2 673 .......................................................................................... 584 N L Y F Q S N A M M T L R T F P M L H D L S D S L F A D R F Query_1 180 CCTGTACTTCCAATCCAATGCCATGATGACTCTCAGAACTTTTCCCATGTTGCACGATCTCTCCGACTCTCTGTTTGCGGACCGTTTTAA 269 1 127 .......................................................................................... 216 2 583 .......................................................................................... 494 0 1 .................................................................... 68 N R I D R L F S Q L T G S T P L P S T P S Y N I R R M G D N Query_1 270 CCGCATCGATCGCCTTTTTAGCCAGCTAACCGGCAGTACACCGTTGCCCTCCACGCCTTCTTACAATATCCGGCGGATGGGTGATAACCG 359 1 217 .......................................................................................... 306 2 493 .......................................................................................... 404 0 69 .......................................................................................... 158 R Y E L T L S V P G W K E S E L E I E T V G G Q L N I S G K Query_1 360 CTATGAACTGACGCTCAGTGTGCCGGGCTGGAAAGAGAGCGAACTGGAAATTGAAACCGTCGGCGGTCAGCTGAACATTAGCGGTAAACG 449 1 307 .......................................................................................... 396 2 403 .......................................................................................... 314 0 159 .......................................................................................... 248 R E E E K T E N G E E G W I H R G I S R S D F R A S Y S L P Query_1 450 TGAAGAGGAGAAAACCGAAAACGGCGAAGAAGGATGGATCCATCGGGGTATCAGCCGCAGCGACTTCCGGGCCAGCTATAGTCTGCCGGA 539 1 397 .......................................................................................... 486 1 440 ............ 429 2 313 .......................................................................................... 224 2 270 ............ 281 0 249 .......................................................................................... 338 0 292 ............ 281 E H V K V T G A S L E N G L L A I E L H Q D I P E E E K P Q Query_1 540 GCATGTGAAAGTGACCGGCGCTTCGCTGGAAAACGGCTTGCTGGCGATTGAATTACACCAGGATATCCCGGAAGAAGAGAAACCGCAGCG 629 1 487 .......................................................................................... 576 2 223 .......................................................................................... 134 0 339 .......................................................................................... 428 R I A I N N N P A I E H K P * H W K W I T D P N S S S V D K Query_1 630 CATCGCCATCAACAACAACCCGGCGATTGAACACAAGCCGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCT 719 1 577 .......................................................................................... 666 2 133 .......................................................................................... 44 0 429 ........................................ 468 L A A A L E H H H H H H * D P A A N K A R K E A E L A A A T Query_1 720 TGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGC 809 1 667 .......................................................................................... 756 2 43 ................................ 12 A E Q * L A * P L G A S K R V L R G F L L K Query_1 810 TGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAA 873 1 757 ................................................................ 820 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2379234 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 2,818 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96877.102 (IDP96877.1-740.F..pMCSG68)
MKKILIMTPDIEGPVRNGGIGTAFTALATTLAKKGYDVDVLYTCGDYSESSVSKFSDWSRIYSTFGINLLRTGLIKEINIDAPYFRRKSYSIYLWLKENNIYDTVISCEWQADLYYTLLSKKNGTDFENTKFIVNTHSSTLWADEGNYQLPYDQNHLELYYMEKMVVEMADEVVSPSQYLIDWMLSKHWNVPEERHVILNCEPFQGFVTRDDVTVKINEKPASGVELVFFGRLETRKGLDIFLRALRKLSDEDKESISGVTFLGKNVTMGKTDSFTYIMNQTKNLGLAVNVISDYDRTNANEYIKRKNVLVIIPSLVENSPYTVYECLINNVNFLASNVGGIPELIPQEHHAEVLFIPTPVDLYGKIHYRLKNINIKPGLAESQDNIKEAWFVAVERKNNRAFKKIDEANSPLVSVCITHFERHHLLQQALASIKSQTYQNIEVILVDDGSTTEDSHRYLNLIENDFNSRGWKIVRSSNNYLGAARNLAARHASGEYLMFMDDDNVAKPFEVETFVTAALNSGADVLTTPSDLIFGEEFPSPFRKMTHCWLPLGPDLNIASFSNCFGDANALIRKEVFEKVGGFTEDYGLGHEDWEFFAKISLQGYKLQIVPEPLFWYRVANSGMLLSGNKSKNNYRSFRPFMDENVKYNYAMGLIPSYLEKIQELESEVNRLRSINGGHSVSNELQLLNNKVDGLISQQRDGWAHDRFNALYEAIHVQGAKRGTSLVRRVARKVKSMLK
ATGAAGAAAATTCTTATAATGACGCCGGACATTGAGGGGCCTGTCCGTAACGGCGGTATTGGTACTGCTTTCACTGCTCTTGCCACTACTTTGGCAAAAAAGGGGTATGATGTTGATGTATTGTATACATGTGGCGACTATTCTGAATCATCTGTATCGAAATTTAGCGACTGGTCACGTATTTATAGTACCTTTGGTATCAATCTGCTAAGAACCGGACTGATAAAAGAGATTAATATTGATGCACCGTATTTTAGAAGGAAAAGTTATTCAATTTATCTCTGGTTGAAAGAAAATAACATCTATGACACTGTTATTTCTTGTGAGTGGCAGGCAGATCTTTATTACACTTTATTAAGCAAAAAGAATGGAACGGATTTTGAAAATACAAAGTTCATTGTAAATACTCACAGTTCAACGTTATGGGCTGATGAAGGTAATTACCAGCTTCCATATGATCAGAACCATCTTGAACTCTATTATATGGAGAAAATGGTGGTTGAAATGGCGGATGAAGTTGTTAGTCCGTCTCAGTATTTAATTGATTGGATGTTGAGTAAGCACTGGAATGTTCCTGAAGAACGTCATGTAATTTTAAATTGCGAGCCATTTCAAGGGTTTGTGACGAGAGATGATGTTACAGTTAAAATAAATGAAAAGCCAGCTTCTGGCGTTGAGCTTGTATTTTTCGGCCGCCTTGAAACCCGTAAAGGACTTGACATATTCCTGCGTGCATTAAGAAAACTATCTGATGAAGATAAAGAGAGCATTTCTGGAGTAACCTTCCTCGGAAAAAATGTCACTATGGGGAAAACTGATTCATTTACTTATATTATGAATCAGACTAAAAATTTGGGACTCGCAGTTAATGTAATCAGCGACTATGATCGTACCAACGCTAATGAATATATAAAAAGAAAAAATGTATTAGTCATCATTCCATCACTTGTAGAAAACTCACCCTATACTGTTTATGAATGCTTGATTAATAACGTTAATTTCCTCGCTTCAAACGTTGGTGGAATTCCAGAGCTTATTCCGCAAGAGCATCATGCGGAAGTTCTATTTATTCCTACACCTGTCGATTTATACGGGAAAATCCACTATCGCTTAAAAAATATAAATATAAAACCAGGGCTTGCTGAATCACAAGACAATATTAAAGAAGCTTGGTTTGTCGCAGTTGAACGAAAAAACAACCGCGCATTCAAGAAAATCGATGAAGCTAACAGCCCGTTAGTTAGCGTGTGTATAACTCACTTCGAACGTCACCATTTGCTTCAGCAAGCACTCGCATCAATAAAATCTCAGACGTACCAAAATATTGAGGTCATCTTGGTTGATGATGGAAGTACGACAGAAGATTCTCATCGTTATTTAAATCTCATCGAGAATGATTTTAACTCTCGAGGCTGGAAAATTGTCCGTAGTTCTAATAACTATCTGGGTGCTGCAAGGAATTTGGCTGCGCGACACGCCTCTGGCGAATATCTGATGTTTATGGACGATGATAATGTTGCTAAGCCTTTTGAGGTAGAAACGTTTGTTACTGCAGCATTAAACTCTGGGGCCGATGTGTTAACCACACCAAGCGATCTTATTTTTGGTGAGGAGTTCCCTTCTCCGTTCCGTAAAATGACGCACTGCTGGCTTCCGTTAGGGCCTGATTTAAATATCGCCAGCTTTAGTAACTGCTTTGGCGATGCTAATGCGCTGATCAGAAAAGAGGTTTTCGAAAAAGTAGGCGGATTTACTGAAGATTACGGTTTAGGTCATGAAGACTGGGAGTTTTTTGCCAAAATATCATTACAGGGATATAAATTGCAAATCGTCCCGGAACCTCTATTTTGGTATAGAGTTGCAAACTCCGGCATGTTGTTAAGTGGAAATAAGAGTAAAAATAACTACCGCAGTTTCCGTCCTTTTATGGATGAGAATGTTAAATATAACTATGCAATGGGGTTGATACCTTCCTACCTCGAGAAGATTCAAGAACTTGAGAGTGAAGTGAATCGCTTGCGGAGCATCAATGGTGGTCATTCTGTCAGTAACGAGTTACAACTTTTAAATAATAAGGTTGATGGTCTTATTTCTCAGCAAAGAGATGGCTGGGCCCATGACCGTTTTAATGCTCTGTATGAAGCAATTCACGTCCAAGGCGCAAAACGAGGCACCAGCCTGGTTCGCCGGGTTGCCCGGAAAGTGAAATCAATGTTAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,708 total letters Query= IDP96877.102 Length=2625 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4004 0.0 2_07A_A1_IDP96877.102_T7term.seq 417 2e-119 1_01A_A1_IDP96877.102_T7S.seq 414 2e-118 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAA 90 2 361 .......................................................................................... 272 2 294 ...G...........T.T.C.C.C...........A... 332 1 3 .................................. 36 E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E N Query_1 91 GAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAAC 180 2 271 .......................................................................................... 182 1 37 .......................................................................................... 126 L Y F Q S N A M K K I L I M T P D I E G P V R N G G I G T A Query_1 181 CTGTACTTCCAATCCAATGCCATGAAGAAAATTCTTATAATGACGCCGGACATTGAGGGGCCTGTCCGTAACGGCGGTATTGGTACTGCT 270 0 1 ..................................................................... 69 2 181 ..................................................A.G... 126 1 127 ..................................................A.G... 182 F T A L A T T L A K K G Y D V D V L Y T C G D Y S E S S V S Query_1 271 TTCACTGCTCTTGCCACTACTTTGGCAAAAAAGGGGTATGATGTTGATGTATTGTATACATGTGGCGACTATTCTGAATCATCTGTATCG 360 0 70 .......................................................................................... 159 0 1803 ..T............ 1789 1 851 ........... 841 K F S D W S R I Y S T F G I N L L R T G L I K E I N I D A P Query_1 361 AAATTTAGCGACTGGTCACGTATTTATAGTACCTTTGGTATCAATCTGCTAAGAACCGGACTGATAAAAGAGATTAATATTGATGCACCG 450 0 160 .......................................................................................... 249 Y F R R K S Y S I Y L W L K E N N I Y D T V I S C E W Q A D Query_1 451 TATTTTAGAAGGAAAAGTTATTCAATTTATCTCTGGTTGAAAGAAAATAACATCTATGACACTGTTATTTCTTGTGAGTGGCAGGCAGAT 540 0 250 .......................................................................................... 339 L Y Y T L L S K K N G T D F E N T K F I V N T H S S T L W A Query_1 541 CTTTATTACACTTTATTAAGCAAAAAGAATGGAACGGATTTTGAAAATACAAAGTTCATTGTAAATACTCACAGTTCAACGTTATGGGCT 630 0 340 .......................................................................................... 429 D E G N Y Q L P Y D Q N H L E L Y Y M E K M V V E M A D E V Query_1 631 GATGAAGGTAATTACCAGCTTCCATATGATCAGAACCATCTTGAACTCTATTATATGGAGAAAATGGTGGTTGAAATGGCGGATGAAGTT 720 0 430 .......................................................................................... 519 V S P S Q Y L I D W M L S K H W N V P E E R H V I L N C E P Query_1 721 GTTAGTCCGTCTCAGTATTTAATTGATTGGATGTTGAGTAAGCACTGGAATGTTCCTGAAGAACGTCATGTAATTTTAAATTGCGAGCCA 810 0 520 .......................................................................................... 609 F Q G F V T R D D V T V K I N E K P A S G V E L V F F G R L Query_1 811 TTTCAAGGGTTTGTGACGAGAGATGATGTTACAGTTAAAATAAATGAAAAGCCAGCTTCTGGCGTTGAGCTTGTATTTTTCGGCCGCCTT 900 0 610 .......................................................................................... 699 E T R K G L D I F L R A L R K L S D E D K E S I S G V T F L Query_1 901 GAAACCCGTAAAGGACTTGACATATTCCTGCGTGCATTAAGAAAACTATCTGATGAAGATAAAGAGAGCATTTCTGGAGTAACCTTCCTC 990 0 700 .......................................................................................... 789 G K N V T M G K T D S F T Y I M N Q T K N L G L A V N V I S Query_1 991 GGAAAAAATGTCACTATGGGGAAAACTGATTCATTTACTTATATTATGAATCAGACTAAAAATTTGGGACTCGCAGTTAATGTAATCAGC 1080 0 790 .......................................................................................... 879 D Y D R T N A N E Y I K R K N V L V I I P S L V E N S P Y T Query_1 1081 GACTATGATCGTACCAACGCTAATGAATATATaaaaagaaaaaaTGTATTAGTCATCATTCCATCACTTGTAGAAAACTCACCCTATACT 1170 0 880 .......................................................................................... 969 V Y E C L I N N V N F L A S N V G G I P E L I P Q E H H A E Query_1 1171 GTTTATGAATGCTTGATTAATAACGTTAATTTCCTCGCTTCAAACGTTGGTGGAATTCCAGAGCTTATTCCGCAAGAGCATCATGCGGAA 1260 0 970 .......................................................................................... 1059 V L F I P T P V D L Y G K I H Y R L K N I N I K P G L A E S Query_1 1261 GTTCTATTTATTCCTACACCTGTCGATTTATACGGGAAAATCCACTATCGCTTAAAAAATATAAATATAAAACCAGGGCTTGCTGAATCA 1350 0 1060 .......................................................................................... 1149 Q D N I K E A W F V A V E R K N N R A F K K I D E A N S P L Query_1 1351 CAAGACAATATTAAAGAAGCTTGGTTTGTCGCAGTTGAACGAAAAAACAACCGCGCATTCAAGAAAATCGATGAAGCTAACAGCCCGTTA 1440 0 1150 .......................................................................................... 1239 V S V C I T H F E R H H L L Q Q A L A S I K S Q T Y Q N I E Query_1 1441 GTTAGCGTGTGTATAACTCACTTCGAACGTCACCATTTGCTTCAGCAAGCACTCGCATCAATAAAATCTCAGACGTACCAAAATATTGAG 1530 0 1240 .......................................................................................... 1329 V I L V D D G S T T E D S H R Y L N L I E N D F N S R G W K Query_1 1531 GTCATCTTGGTTGATGATGGAAGTACGACAGAAGATTCTCATCGTTATTTAAATCTCATCGAGAATGATTTTAACTCTCGAGGCTGGAAA 1620 0 1330 .......................................................................................... 1419 I V R S S N N Y L G A A R N L A A R H A S G E Y L M F M D D Query_1 1621 ATTGTCCGTAGTTCTAATAACTATCTGGGTGCTGCAAGGAATTTGGCTGCGCGACACGCCTCTGGCGAATATCTGATGTTTATGGACGAT 1710 0 1420 .......................................................................................... 1509 D N V A K P F E V E T F V T A A L N S G A D V L T T P S D L Query_1 1711 GATAATGTTGCTAAGCCTTTTGAGGTAGAAACGTTTGTTACTGCAGCATTAAACTCTGGGGCCGATGTGTTAACCACACCAAGCGATCTT 1800 0 1510 .......................................................................................... 1599 I F G E E F P S P F R K M T H C W L P L G P D L N I A S F S Query_1 1801 ATTTTTGGTGAGGAGTTCCCTTCTCCGTTCCGTAAAATGACGCACTGCTGGCTTCCGTTAGGGCCTGATTTAAATATCGCCAGCTTTAGT 1890 0 1600 .......................................................................................... 1689 2 538 ........... 548 N C F G D A N A L I R K E V F E K V G G F T E D Y G L G H E Query_1 1891 AACTGCTTTGGCGATGCTAATGCGCTGATCAGAAAAGAGGTTTTCGAAAAAGTAGGCGGATTTACTGAAGATTACGGTTTAGGTCATGAA 1980 0 1690 .......................................................................................... 1779 D W E F F A K I S L Q G Y K L Q I V P E P L F W Y R V A N S Query_1 1981 GACTGGGAGTTTTTTGCCAAAATATCATTACAGGGATATAAATTGCAAATCGTCCCGGAACCTCTATTTTGGTATAGAGTTGCAAACTCC 2070 0 1780 .......................................................................................... 1869 0 101 ............G.. 87 G M L L S G N K S K N N Y R S F R P F M D E N V K Y N Y A M Query_1 2071 GGCATGTTGTTAAGTGGAAATAAGAGTAAAAATAACTACCGCAGTTTCCGTCCTTTTATGGATGAGAATGTTAAATATAACTATGCAATG 2160 0 1870 .......................................................................................... 1959 G L I P S Y L E K I Q E L E S E V N R L R S I N G G H S V S Query_1 2161 GGGTTGATACCTTCCTACCTCGAGAAGATTCAAGAACTTGAGAGTGAAGTGAATCGCTTGCGGAGCATCAATGGTGGTCATTCTGTCAGT 2250 0 1960 .......................................................................................... 2049 N E L Q L L N N K V D G L I S Q Q R D G W A H D R F N A L Y Query_1 2251 AACGAGTTACAACTTTTAAATAATAAGGTTGATGGTCTTATTTCTCAGCAAAGAGATGGCTGGGCCCATGACCGTTTTAATGCTCTGTAT 2340 0 2050 .......................................................................................... 2139 E A I H V Q G A K R G T S L V R R V A R K V K S M L K * H W Query_1 2341 GAAGCAATTCACGTCCAAGGCGCAAAACGAGGCACCAGCCTGGTTCGCCGGGTTGCCCGGAAAGTGAAATCAATGTTAAAATAACATTGG 2430 0 2140 ................................................................................. 2220 2 136 .................................. 103 1 172 .................................. 205 K W I T D P N S S S V D K L A A A L E H H H H H H * D P A A Query_1 2431 AAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCT 2520 2 102 ....................................................................................... 16 1 206 .......................................................................................... 295 N K A R K E A E L A A A T A E Q * L A * P L G A S K R V L R Query_1 2521 AACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGG 2610 1 296 .......................................................................................... 385 G F L L K Query_1 2611 GGTTTTTTGCTGAAA 2625 1 386 ............... 400 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12145456 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 4,708 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96878.103 (IDP96878.1-342.F..pMCSG73)
MITMTIKTYREAVKEALAQEMERDERVVLIGEDLRGGHGGNAPEEAKIEAFGGVLGVTKGLWTQFGSDRVIDTPITESAIIGMAAGAAATGLRPVAELMFMDFFGVSHDALYNQAAKFRYMFGGKARAPLVMRGMIGAGFSAAAQHSQSPYNIFATTPGLKVVVPSTPYDVKGLLIQSIRDDDPVVFCEHKMLYDLKGEVPDEIYTIPLGVANYTREGEDVTIIALSAMVHKANQVADKLAKEGISVEVVDPRTISPLDEEGILESVASTGRVVIVDESAARFGFAHDVAALIASQAFHFLKAPVLLVTPPHTPVPFSPALEKLWIPGVERIEAAVRQVLED
GTGATAACCATGACAATAAAAACCTATCGTGAAGCGGTCAAGGAAGCCCTGGCTCAGGAAATGGAACGCGATGAACGCGTGGTGCTCATCGGTGAAGACTTGCGTGGCGGTCATGGCGGAAATGCGCCCGAAGAGGCGAAAATAGAAGCCTTTGGCGGTGTGCTGGGTGTCACTAAAGGGCTGTGGACGCAGTTCGGCTCCGATCGGGTGATCGACACGCCCATTACCGAGTCGGCCATCATCGGTATGGCGGCCGGCGCCGCAGCGACGGGTTTGCGGCCGGTCGCGGAATTGATGTTCATGGATTTTTTTGGCGTGAGTCACGATGCGCTGTACAACCAGGCGGCTAAGTTCCGCTACATGTTTGGTGGCAAAGCCAGAGCTCCGCTGGTGATGCGAGGGATGATCGGCGCGGGGTTTTCTGCCGCAGCCCAGCATTCACAGTCACCCTATAATATCTTTGCCACCACGCCTGGACTGAAGGTGGTGGTGCCCTCGACGCCTTATGACGTCAAAGGTCTGTTAATCCAGTCCATTCGCGACGACGACCCGGTGGTATTCTGCGAGCATAAAATGCTGTACGACCTCAAGGGCGAGGTACCGGACGAGATCTATACCATCCCGCTAGGTGTAGCCAACTACACGCGCGAAGGAGAGGACGTCACCATCATTGCGCTGTCGGCAATGGTGCATAAAGCCAACCAGGTGGCGGACAAGCTGGCGAAAGAGGGGATCTCGGTCGAGGTGGTCGACCCGCGAACCATTTCGCCGCTGGATGAGGAAGGGATTCTGGAATCGGTGGCGTCCACGGGGCGGGTCGTGATTGTCGACGAATCCGCGGCACGCTTCGGTTTTGCTCATGATGTCGCAGCGCTGATTGCGTCCCAGGCATTCCATTTCCTCAAAGCGCCCGTTCTGCTGGTGACGCCGCCACACACGCCGGTCCCGTTCTCCCCTGCTCTCGAAAAACTCTGGATCCCTGGCGTAGAACGTATCGAAGCCGCCGTCCGTCAAGTGCTGGAGGAT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,054 total letters Query= IDP96878.103 Length=1431 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1851 0.0 2_10F_F4_IDP96878.103_T7_Term.seq 1748 0.0 1_04F_F4_IDP96878.103_v73F.seq 1245 0.0 S H P Q F E K G T E N L Y F Q S N A V I T M T I K T Y Query_1 148 TCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAAT--CCAATGCCGT-GATAA-CCATGAC-AATAAAAA--CCTAT-C 229 0 1 ..-.....-.......-........--.....-. 28 2 1102 .....TC........N.T.....N.....N.C........AC.....T. 1054 1 37 ........A.......G.G.A..-----..................--........A.-.....-.......-........--.....-. 113 R E A V K E A L A Q E M E R D E R V V L I G E D L R G Query_1 230 GTGAAGCGGT-CAAGGAA--GCCCTGGCT--CAGGAAATGGAACGCGATGAACGCGTGGTGCTCAT-CGGTG-AAGACTTGCGTGG--CG 310 0 29 ..........-.......--.........--...................................-.....-.............--.. 109 0 902 ...........T.. 889 2 1053 NN.T......T.....N.AG.........TC...........CN......................T.....G...........T.GC.. 964 2 169 ...........T.. 182 1 114 ...T......-.......--.........--...................................-.....-.............--.. 194 G H G G N A P E E A K I E A F G G V L G V T K G L W T Q F G Query_1 311 GTCATGGCGGAAATGCGCCCGAAGAGGCGAAAATAGAAGCCTTTGGCGGTGTGCTGGGTGTCACT-AAAGGGCTGTGGACGCAGTTCGGC 399 0 110 .................................................................-........................ 198 2 963 .........................N.................N.............-.......N........................ 875 1 195 .................................................................-........................ 283 S D R V I D T P I T E S A I I G M A A G A A A T G L R P V Query_1 400 TCCGATCGGGTGA-TCGACACGCCCATTACCGAGTCGGCCATCATCGGTATGGCGGCCGGCGCCGCAGCGACGGGTTTGCGGCCGGTCGC 488 0 199 .............-............................................................................ 287 2 874 .............A.........................................C.........................N........ 785 1 284 .............-............................................................................ 372 A E L M F M D F F G V S H D A L Y N Q A A K F R Y M F G G K Query_1 489 GGAATTGATGTTCATGGAtttttttGGCGTGAGTCACGATGCGCTGTACAACCAGGCGGCTAAGTTCCGCTACA-TGTTTGGTGGCAAAG 577 0 288 ..........................................................................-............... 376 0 469 ........... 459 2 784 ..........................................................................A............... 695 2 602 ........... 612 1 373 ..........................................................................-............... 461 1 554 ........... 544 A R A P L V M R G M I G A G F S A A A Q H S Q S P Y N I F A Query_1 578 CCAGAGCTCCGCTGGTGATGCGAGGGATGATCGGCGCGGGGTTTTCTGCCGCAGCCCAGCATTCACAGTCACCCTATAATATCTTTGCCA 667 0 377 .......................................................................................... 466 0 376 ........ 369 2 694 .......................................................................................... 605 2 695 ........ 702 1 462 .....................................................N.................................... 551 1 461 ........ 454 T T P G L K V V V P S T P Y D V K G L L I Q S I R D D D P V Query_1 668 CCACGCCTGGACTGAAGGTGGTGGTGCCCTCGACGCCTTATGACGTCAAAGGTCTGTTAATCCAGTCCATTCGCGACGACGACCCGGTGG 757 0 467 .......................................................................................... 556 0 368 ... 366 2 604 .......................................................................................... 515 2 703 ... 705 1 552 ...............................................N................N...............N.....N... 641 1 453 ... 451 V F C E H K M L Y D L K G E V P D E I Y T I P L G V A N Y T Query_1 758 TATTCTGCGAGCATAAAATGCTGTACGACCTCAAGGGCGAGGTACCGGACGAGATCTATACCATCCCGCTAGGTGTAGCCAACTACACGC 847 0 557 .......................................................................................... 646 2 514 .......................................................................................... 425 1 642 .......................................................................................... 731 R E G E D V T I I A L S A M V H K A N Q V A D K L A K E G I Query_1 848 GCGAAGGAGAGGACGTCACCATCATTGCGCTGTCGGCAATGGTGCATAAAGCCAACCAGGTGGCGGACAAGCTGGCGAAAGAGGGGATCT 937 0 647 .......................................................................................... 736 2 424 .......................................................................................... 335 1 732 ........T...................N... 763 S V E V V D P R T I S P L D E E G I L E S V A S T G R V V I Query_1 938 CGGTCGAGGTGGTCGACCCGCGAACCATTTCGCCGCTGGATGAGGAAGGGATTCTGGAATCGGTGGCGTCCACGGGGCGGGTCGTGATTG 1027 0 737 .......................................................................................... 826 2 334 .......................................................................................... 245 V D E S A A R F G F A H D V A A L I A S Q A F H F L K A P V Query_1 1028 TCGACGAATCCGCGGCACGCTTCGGTTTTGCTCATGATGTCGCAGCGCTGATTGCGTCCCAGGCATTCCATTTCCTCAAAGCGCCCGTTC 1117 0 827 .......................................................................................... 916 0 69 ..G........... 56 2 244 .......................................................................................... 155 1 154 ..G........... 141 L L V T P P H T P V P F S P A L E K L W I P G V E R I E A A Query_1 1118 TGCTGGTGACGCCGCCACACACGCCGGTCCCGTTCTCCCCTGCTCTCGAAAAACTCTGGATCCCTGGCGTAGAACGTATCGAAGCCGCCG 1207 0 917 .......................................................................................... 1006 2 154 .......................................................................................... 65 V R Q V L E D * H W K W I T D P Query_1 1208 TCCGTCAAGTGCTGGAGGATTAACATTGGAAGTGGATAACGGATCCG 1254 0 1007 .................... 1026 2 64 ........................................--..... 20 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4253490 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 3,054 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96881.103 (IDP96881.34-222.P(222)..pMCSG73)
KDYQAGKNFTVIHSTVKQPPPLVEFFSFYCGPCYAFAERINVDTAIRKRLPDDMKLEKYHVSQMGPLGPALTEAWAVAQYAGVDGKVEKLLFEGLQVKRDIKTAADIVKVFNQLGITSEKYAEMQSNFMVKALIARQDNLVEKMKVHGTPSFYVSGKYHINNASLAQDDYDTYAEDMANLVLFLLNKPL
AAAGATTATCAGGCAGGGAAAAACTTCACTGTTATTCATTCTACTGTTAAGCAACCGCCGCCACTGGTTGAGTTTTTTTCCTTCTACTGCGGACCTTGTTATGCGTTTGCAGAGCGCATCAATGTTGATACAGCTATCAGAAAGCGCCTGCCCGACGATATGAAACTGGAAAAATACCATGTCAGCCAGATGGGGCCTTTAGGACCAGCATTAACAGAAGCATGGGCCGTAGCACAATATGCTGGGGTAGATGGCAAGGTGGAAAAGTTATTGTTTGAGGGTTTACAGGTTAAGCGTGATATTAAAACGGCAGCTGATATTGTTAAGGTGTTTAATCAACTTGGCATTACGTCAGAGAAATATGCCGAAATGCAAAGTAACTTTATGGTAAAAGCTTTAATTGCCAGACAGGATAATCTGGTCGAAAAAATGAAAGTGCATGGCACGCCTTCTTTTTATGTTTCAGGAAAATATCATATTAACAATGCATCACTCGCGCAAGATGACTATGATACCTATGCAGAGGATATGGCTAATCTGGTTTTATTTTTGCTTAACAAGCCCCTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,047 total letters Query= IDP96881.103 Length=972 Score E Sequences producing significant alignments: (Bits) Value 2_10G_G4_IDP96881.103_T7_Term.seq 1128 0.0 1_04G_G4_IDP96881.103_v73F.seq 1128 0.0 0_wt 1023 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 2 685 ...........A........G....------....................... 638 2 703 .....C........... 687 1 45 ...........A........G....------....................... 92 1 28 ...C........... 42 A K D Y Q A G K N F T V I H S T V K Q P P P L V E F F S F Y Query_1 199 GCCAAAGATTATCAGGCAGGGAAAAACTTCACTGTTATTCATTCTACTGTTAAGCAACCGCCGCCACTGGTTGAGtttttttCCTTCTAC 288 2 637 .......................................................................................... 548 1 93 .......................................................................................... 182 0 1 ....................................................................................... 87 C G P C Y A F A E R I N V D T A I R K R L P D D M K L E K Y Query_1 289 TGCGGACCTTGTTATGCGTTTGCAGAGCGCATCAATGTTGATACAGCTATCAGAAAGCGCCTGCCCGACGATATGAAACTGGAAAAATAC 378 2 547 .......................................................................................... 458 1 183 .......................................................................................... 272 0 88 .......................................................................................... 177 H V S Q M G P L G P A L T E A W A V A Q Y A G V D G K V E K Query_1 379 CATGTCAGCCAGATGGGGCCTTTAGGACCAGCATTAACAGAAGCATGGGCCGTAGCACAATATGCTGGGGTAGATGGCAAGGTGGAAAAG 468 2 457 .......................................................................................... 368 1 273 .......................................................................................... 362 0 178 .......................................................................................... 267 L L F E G L Q V K R D I K T A A D I V K V F N Q L G I T S E Query_1 469 TTATTGTTTGAGGGTTTACAGGTTAAGCGTGATATTAAAACGGCAGCTGATATTGTTAAGGTGTTTAATCAACTTGGCATTACGTCAGAG 558 2 367 .......................................................................................... 278 1 363 .......................................................................................... 452 0 268 .......................................................................................... 357 K Y A E M Q S N F M V K A L I A R Q D N L V E K M K V H G T Query_1 559 AAATATGCCGAAATGCAAAGTAACTTTATGGTAAAAGCTTTAATTGCCAGACAGGATAATCTGGTCGAAAAAATGAAAGTGCATGGCACG 648 2 277 .......................................................................................... 188 1 453 .......................................................................................... 542 0 358 .......................................................................................... 447 P S F Y V S G K Y H I N N A S L A Q D D Y D T Y A E D M A N Query_1 649 CCTTCTTTTTATGTTTCAGGAAAATATCATATTAACAATGCATCACTCGCGCAAGATGACTATGATACCTATGCAGAGGATATGGCTAAT 738 2 187 .......................................................................................... 98 1 543 .......................................................................................... 632 0 448 .......................................................................................... 537 L V L F L L N K P L * H W K W I T D P N S S S V D K L A A A Query_1 739 CTGGTTTTATTTTTGCTTAACAAGCCCCTGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCA 828 2 97 ..................................................--..... 43 1 633 ..................................................--..... 687 0 538 .............................. 567 L E H H H H H H * D P A A N K A R K E A E L A A A T A E Q Query_1 829 CTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAA--GCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAAT 916 2 47 .................AGC........... 17 1 683 .................--........................................... 742 * L A * P L G A S K R V L R G F L L Query_1 917 AACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAA 971 1 743 ....................................................... 797 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2872914 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 3,047 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96883.102 (IDP96883.1-123.F..pMCSG68)
MSWNPETPYNDLPIPPPDLERVETRNVLNACISARAAIAKLKTVSELIPYQRVLINILPMLETKDSSRIENIITTSDQLFQYADRASGADPATKEALRYRTALYDGYIHLEAYQLCENTAITI
ATGAGCTGGAATCCAGAGACCCCTTACAACGATCTGCCGATACCGCCGCCCGATCTGGAGCGCGTTGAAACACGCAATGTACTCAACGCATGCATAAGTGCGCGTGCAGCAATTGCAAAGTTGAAAACTGTCAGTGAGCTGATCCCCTATCAGAGGGTACTGATAAACATTCTCCCGATGCTGGAAACGAAAGATTCTTCACGCATTGAGAACATCATAACGACCAGCGATCAGCTCTTTCAATATGCTGACCGCGCAAGTGGTGCAGATCCAGCCACGAAAGAAGCACTGCGCTATCGTACGGCGCTTTATGACGGTTATATCCACCTTGAGGCATATCAGCTCTGTGAAAACACCGCGATAACTATC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,778 total letters Query= IDP96883.102 Length=774 Score E Sequences producing significant alignments: (Bits) Value 1_01F_F1_IDP96883.102_T7S.seq 1294 0.0 2_07F_F1_IDP96883.102_T7term.seq 1213 0.0 0_wt 666 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAA 90 1 2 ..T................................. 37 2 706 .......................................................................................... 617 2 639 ...G...........T.T.C.C.C...........A... 677 E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E N Query_1 91 GAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAAC 180 1 38 .......................................................................................... 127 2 616 .......................................................................................... 527 L Y F Q S N A M S W N P E T P Y N D L P I P P P D L E R V E Query_1 181 CTGTACTTCCAATCCAATGCCATGAGCTGGAATCCAGAGACCCCTTACAACGATCTGCCGATACCGCCGCCCGATCTGGAGCGCGTTGAA 270 1 128 .......................................................................................... 217 2 526 .......................................................................................... 437 0 1 ..................................................................... 69 T R N V L N A C I S A R A A I A K L K T V S E L I P Y Q R V Query_1 271 ACACGCAATGTACTCAACGCATGCATAAGTGCGCGTGCAGCAATTGCAAAGTTGAAAACTGTCAGTGAGCTGATCCCCTATCAGAGGGTA 360 1 218 .......................................................................................... 307 2 436 .......................................................................................... 347 0 70 .......................................................................................... 159 L I N I L P M L E T K D S S R I E N I I T T S D Q L F Q Y A Query_1 361 CTGATAAACATTCTCCCGATGCTGGAAACGAAAGATTCTTCACGCATTGAGAACATCATAACGACCAGCGATCAGCTCTTTCAATATGCT 450 1 308 .......................................................................................... 397 2 346 .......................................................................................... 257 0 160 .......................................................................................... 249 D R A S G A D P A T K E A L R Y R T A L Y D G Y I H L E A Y Query_1 451 GACCGCGCAAGTGGTGCAGATCCAGCCACGAAAGAAGCACTGCGCTATCGTACGGCGCTTTATGACGGTTATATCCACCTTGAGGCATAT 540 1 398 .......................................................................................... 487 2 256 .......................................................................................... 167 0 250 .......................................................................................... 339 Q L C E N T A I T I * H W K W I T D P N S S S V D K L A A A Query_1 541 CAGCTCTGTGAAAACACCGCGATAACTATCTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCA 630 1 488 .......................................................................................... 577 2 166 .......................................................................................... 77 0 340 .............................. 369 L E H H H H H H * D P A A N K A R K E A E L A A A T A E Q Query_1 631 CTCGAGcaccaccaccaccaccacTG-AGATCCGGCTGCT-AACAAAG-CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAA 717 1 578 ..........................-.............-.......-......................................... 664 2 76 ..........................A.............A.......C....... 21 * L A * P L G A S K R V L R G F L L K Query_1 718 TAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAA 774 1 665 ......................................................... 721 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2074347 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 2,778 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96884.102 (IDP96884.21-274.P(274)..pMCSG68)
STDCGAGSPSAETIAATMHQAQRLKQQLNSEPEPVVILVRQGQDMSSRGLTWSHAGYALRQADNSWRVYHNLNTCGTARSALYVQGLYEFLADELVNQQIAVLRPRGELNAALQELLHSPVKLNLFHSPRYNLIAWPFSGPYQNSNGWLLELFARANDSHIWSRQQARDWLAQESYQPSLVNVTLFERLGAKVVASNVFTDDQPDELLAQGKVGLNSGDSVIRFIARYSRAIPGCQHLNLPDSVCVFIPEHQVQ
AGCACCGATTGCGGCGCGGGGTCGCCATCGGCAGAAACTATCGCCGCCACCATGCATCAGGCGCAACGGCTCAAACAGCAATTGAACAGCGAGCCGGAACCGGTGGTTATTTTAGTGCGCCAGGGTCAGGATATGTCCAGCCGCGGGCTGACCTGGTCTCACGCAGGTTACGCCCTCCGACAGGCCGATAATAGCTGGCGGGTGTACCATAACCTGAATACCTGCGGTACGGCGCGGTCAGCGCTGTACGTGCAGGGGCTCTATGAGTTTCTCGCCGATGAACTGGTTAACCAGCAGATAGCGGTTTTACGCCCGCGCGGCGAACTTAACGCCGCCTTACAAGAGCTTTTACACAGTCCTGTCAAACTGAACCTGTTCCACAGCCCGCGCTATAACCTGATCGCCTGGCCCTTCTCCGGGCCGTATCAAAACTCTAACGGCTGGCTGCTGGAACTCTTCGCCCGCGCCAACGACAGCCATATCTGGTCACGTCAGCAGGCCAGAGACTGGCTGGCGCAGGAGTCGTACCAACCCTCGCTGGTCAACGTCACGCTTTTTGAGCGCCTGGGCGCAAAGGTGGTGGCCAGCAATGTTTTTACCGACGATCAGCCGGATGAGCTATTGGCGCAAGGTAAAGTGGGTTTAAATAGCGGCGATAGCGTGATTCGGTTTATCGCGCGTTACAGCCGGGCGATACCCGGCTGTCAGCATCTGAATCTACCCGACTCTGTTTGCGTTTTTATACCTGAACACCAGGTTCAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,200 total letters Query= IDP96884.102 Length=1167 Score E Sequences producing significant alignments: (Bits) Value 1_01G_G1_IDP96884.102_T7S.seq 1902 0.0 2_07G_G1_IDP96884.102_T7term.seq 1824 0.0 0_wt 1375 0.0 I N T T H Y R G I V S G * Q F P S R N N F V * L * E Query_1 14 AAATTAA-TACG-ACTCACTA-TA--GGGGAATTGTGA--GCGG--ATAACAATTCCCCTCTAGAAA-TAATTTTGTTTAACTTTAAGAA 93 1 5 ..............-...................... 40 2 1095 .......A....N.N..N...A..AG...........GAG....GA..............N......A...................... 1006 2 1032 ...-N.--.......... 1046 G D I H M H H H H H H S S G V D L W S H P Q F E K G T E N Query_1 94 GGAGATATACATA-TGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGG-TCTCATCCGCAGTTCGAAAAGGGTACCGAGAACC 181 1 41 .............-.........................................-.................................. 128 2 1005 .............A.........................................G............G..................... 916 L Y F Q S N A S T D C G A G S P S A E T I A A T M H Q A Q R Query_1 182 TGTACTTCCAATCCAATGCCAGCACCGATTGCGGCGCGGGGTCGCCATCGGCAGAAACTATCGCCGCCACCATGCATCAGG-CGCAACGG 270 1 129 .................................................................................-........ 217 2 915 .................................................................................G........ 826 0 1 .............................................................-........ 69 L K Q Q L N S E P E P V V I L V R Q G Q D M S S R G L T W S Query_1 271 CTCAAACAGCAATTGAACAGCGAGCCGGAACCGGTGGTTATTTTAGTGCGCCAGGGTCAGGATATGTCCAGCCGCGGGCTGACCTGGTCT 360 1 218 .......................................................................................... 307 2 825 .......................................................................................... 736 0 70 .......................................................................................... 159 H A G Y A L R Q A D N S W R V Y H N L N T C G T A R S A L Y Query_1 361 CACGCAGGTTACGCCCTCCGACAGGCCGATAATAGCTGGCGGGTGTACCATAACCTGAATACCTGCGGTACGGCGCGGTCAGCGCTGTAC 450 1 308 .......................................................................................... 397 2 735 .......................................................................................... 646 0 160 .......................................................................................... 249 V Q G L Y E F L A D E L V N Q Q I A V L R P R G E L N A A L Query_1 451 GTGCAGGGGCTCTATGAGTTTCTCGCCGATGAACTGGTTAACCAGCAGATAGCGGTTTTACGCCCGCGCGGCGAACTTAACGCCGCCTTA 540 1 398 .......................................................................................... 487 1 442 ............ 431 2 645 .......................................................................................... 556 2 601 ............ 612 0 250 .......................................................................................... 339 0 294 ............ 283 Q E L L H S P V K L N L F H S P R Y N L I A W P F S G P Y Q Query_1 541 CAAGAGCTTTTACACAGTCCTGTCAAACTGAACCTGTTCCACAGCCCGCGCTATAACCTGATCGCCTGGCCCTTCTCCGGGCCGTATCAA 630 1 488 .......................................................................................... 577 2 555 .......................................................................................... 466 0 340 .......................................................................................... 429 N S N G W L L E L F A R A N D S H I W S R Q Q A R D W L A Q Query_1 631 AACTCTAACGGCTGGCTGCTGGAACTCTTCGCCCGCGCCAACGACAGCCATATCTGGTCACGTCAGCAGGCCAGAGACTGGCTGGCGCAG 720 1 578 .......................................................................................... 667 2 465 .......................................................................................... 376 0 430 .......................................................................................... 519 E S Y Q P S L V N V T L F E R L G A K V V A S N V F T D D Q Query_1 721 GAGTCGTACCAACCCTCGCTGGTCAACGTCACGCTTTTTGAGCGCCTGGGCGCAAAGGTGGTGGCCAGCAATGTTTTTACCGACGATCAG 810 1 668 .......................................................................................... 757 2 375 .......................................................................................... 286 0 520 .......................................................................................... 609 P D E L L A Q G K V G L N S G D S V I R F I A R Y S R A I Query_1 811 CCGGATGAGCTATTGGCGCAAGGTAAAGTGGG-TTTAAATAGCGGCGATAGCGTGATTCGGTTTATCGCGCGTTACAGCCGGGCGATACC 899 1 758 ................................T......................................................... 847 2 285 ................................-......................................................... 197 0 610 ................................-......................................................... 698 P G C Q H L N L P D S V C V F I P E H Q V Q * H W K W I T D Query_1 900 CGGCTGTCAGCATCTGAATCTACCCGACTCTGTTTGCGTTTTTATACCTGAACACCAGGTTCAATAACATT-GGAAGTGGATAACGGATC 988 1 848 .......................................................................G.................. 937 2 196 .......................................................................-.................. 108 0 699 ................................................................ 762 P N S S S V D K L A A A L E H H H H H H * D P A A N K A R Query_1 989 CGAATTCGAGCTCCG-TCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCC--GAA 1075 1 938 ...............T.....................................N.....................-.........--... 1024 2 107 ...............-.....................................................................CGA.. 19 K E A E L A A A T A E Q * L A * P L G A S K R V L R G F L L Query_1 1076 AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGA 1165 1 1025 .....................-.NN...C........NT............-.........CT...N..N.N......--.......... 1110 2 18 ..... 14 Query_1 1166 A 1166 1 1111 . 1111 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3627952 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 3,200 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96885.102 (IDP96885.1-441.F..pMCSG68)
MSKATEQNDKLKRAIIISVALHIILIALLIWSSFDEHLDASAGGGGGSSIDAVMVDPGAVVNNYNRQQQQQASARRAAEQREKQAQQQAEELREKQAAEQERLKQLEQERLQAQEAAKEAKEQQKQAEEAAAKAAAAAKAKADAQAKEAQEAAAKAAAEAKAKADAQAKAAEQAAAKAAADAKKQAEAAAAKAAAEAKKQAEAEAAKAAAEAQKKAEAAAAKKAQQEAEKKAQQEAAKQAAAEKAAAEKAAEKAAAQKAAAEKAAAEKAAAAEKAAAAKAAAAEKAAADKAAKAAAAKAAAAKKAAAAKEADGVDNLLGDLSSGKNAPKTGGGAKGNNASAAGSGNTKNSASGADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGLLLNIQSEGGDPALCQAALAAARQAKFPKPPSQAVYEVFKNAPLDFKPQ
GTGTCAAAGGCAACCGAACAAAACGACAAGCTTAAACGAGCGATCATCATTTCAGTCGCGCTGCACATCATTCTGATCGCGCTGCTGATCTGGAGTTCGTTTGACGAGCATCTGGATGCCTCTGCCGGCGGCGGCGGCGGATCGTCGATTGATGCCGTCATGGTCGATCCGGGGGCGGTGGTAAATAACTATAACCGTCAGCAACAGCAGCAGGCCAGCGCACGTCGCGCCGCTGAACAGCGTGAAAAACAGGCGCAGCAGCAGGCGGAAGAGTTACGTGAGAAACAGGCGGCGGAACAGGAACGGCTGAAACAGCTCGAACAGGAGCGGCTGCAGGCGCAGGAAGCGGCGAAAGAAGCGAAGGAGCAGCAGAAGCAGGCTGAAGAAGCGGCTGCCAAGGCCGCCGCGGCGGCAAAAGCCAAAGCGGACGCACAGGCAAAAGAAGCGCAGGAAGCCGCTGCCAAAGCGGCCGCCGAGGCGAAAGCGAAGGCGGATGCCCAGGCGAAAGCGGCAGAACAGGCGGCGGCCAAGGCGGCTGCTGACGCGAAAAAGCAGGCCGAAGCCGCTGCAGCGAAAGCCGCTGCCGAGGCGAAGAAACAGGCGGAAGCTGAAGCGGCGAAAGCTGCGGCCGAGGCGCAGAAGAAAGCGGAAGCGGCGGCTGCGAAGAAAGCGCAACAGGAAGCGGAGAAAAAAGCCCAGCAGGAAGCGGCTAAGCAGGCGGCAGCTGAAAAAGCGGCTGCCGAAAAAGCCGCTGAGAAAGCCGCCGCGCAAAAAGCGGCCGCTGAGAAGGCCGCCGCCGAGAAAGCCGCAGCCGCTGAAAAAGCGGCGGCAGCGAAAGCGGCTGCAGCAGAGAAGGCTGCAGCTGATAAAGCGGCCAAAGCGGCAGCAGCAAAAGCCGCGGCGGCGAAGAAAGCGGCGGCTGCGAAAGAAGCGGACGGCGTTGACAACCTGCTCGGCGATCTGAGTTCTGGTAAGAATGCGCCTAAAACAGGCGGTGGGGCCAAAGGAAACAATGCCTCCGCTGCCGGGAGTGGTAATACTAAAAACAGTGCCTCAGGGGCTGATATCAACAACTATGCCGGACAGATAAAATCGGCGATTGAAAGTAAGTTTTATGACGCATCGTCCTATGCGGGCAAAACATGTACCTTGCGTATCAAACTTGCTCCTGACGGCCTGTTGTTAAATATACAGTCCGAAGGTGGTGATCCTGCTCTGTGCCAGGCCGCTCTTGCCGCAGCCCGACAGGCTAAGTTTCCGAAACCACCTAGCCAGGCAGTATATGAAGTCTTCAAAAATGCGCCACTGGACTTCAAACCTCAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 1,333 total letters Query= IDP96885.102 Length=1728 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2387 0.0 V S K A T E Q N D K L K R A I I I S V A L H I I L I A L L I Query_1 202 GTGTCAAAGGCAACCGAACAAAACGACAAGCTTAAACGAGCGATCATCATTTCAGTCGCGCTGCACATCATTCTGATCGCGCTGCTGATC 291 0 1 .......................................................................................... 90 W S S F D E H L D A S A G G G G G S S I D A V M V D P G A V Query_1 292 TGGAGTTCGTTTGACGAGCATCTGGATGCCTCTGCCGGCGGCGGCGGCGGATCGTCGATTGATGCCGTCATGGTCGATCCGGGGGCGGTG 381 0 91 .......................................................................................... 180 V N N Y N R Q Q Q Q Q A S A R R A A E Q R E K Q A Q Q Q A E Query_1 382 GTAAATAACTATAACCGTCAGCAACAGCAGCAGGCCAGCGCACGTCGCGCCGCTGAACAGCGTGAAAAACAGGCGCAGCAGCAGGCGGAA 471 0 181 .......................................................................................... 270 E L R E K Q A A E Q E R L K Q L E Q E R L Q A Q E A A K Query_1 472 GAGTTACGTGAGAAACAGGCGGCGGAACAGGAACGGCTGAAACAGCTCGAACAGGAGCGGCTGCAG--GCGCAG---GAAGCGGCGAAAG 556 0 271 ..................................................................--......---............. 355 0 610 ............. 622 0 837 ...........CA.A.A..GCT.C...T.AT.... 871 0 676 ...---.......A....A 691 0 919 ..T....... 928 0 515 ............ 526 0 593 ............ 604 0 699 ....---........ 710 E A K E Q Q K Q A E E A A A K A A A A A K A K A Query_1 557 AAGCGAAGGAGCA-GCAG--AAGCAGGC------TGAAGAAGCGGCTGCCAAG---GC------CGCCGCGGCGGCAAAAGCCAAAGCGG 628 0 356 .............-....--........------...................---..------.......................... 427 0 623 CT...GCC...GC-....--...A.A..------G....CG........G...AAA..GCAACAG.AA....A.AA.......C.GCA.. 703 0 872 CG..C..--...G-....--C....----------A...CC.....G..G...---AA------A..G.....T..G....---...... 934 0 692 ....CC..C..G.A..G.CT........GGCAGC....A...........G.A---AA------A.....T.A.---......---..C. 766 0 778 ..C....AG...---..------...... 797 0 929 ..... 933 0 413 ..------............ 400 0 895 ...........G..---G....... 916 0 905 ..------......... 895 D A Q A K E A Q E A A A K A A A E A K A K A D A Q A K Query_1 629 ACGCACAGGCAAAAGAAG-CGC--AGGAAGCCGCTGCC---AAAGCGGCCGCCGAGGCGAAAGCGAAGGCGGATGCCCAGGCG---AAAG 709 0 428 ..................-...--..............---..........................................---.... 508 0 704 .A..---...T..-------..--...CG..A....AA---........T.....---A.....CGCT.A.A.A...GCC...CAA.... 775 0 935 ... 937 0 767 -....-----.....CG.C...TG..A.G.....C...GAG.....C..A....CT.AA.....---.....-----..-...---.... 838 0 871 ..G...---........A.. 887 0 917 CG..---T..G.......-.. 933 A A E Q A A A K A A A D A K K Q A E A A A A K A A A Query_1 710 CG---GCAGAACAGGCGGCGGCCAAGGCGGCTGCTGACGC-GAAAAAGCAG-----GCCGAAGCCGCTGCAGC-GA-AAGCCGCTGCCGA 788 0 509 ..---...................................-..........-----.................-..-............. 587 0 776 ..GCC..T..GA....C..C...G..AAA..C..A.C...T........G.CGGCA..GA....G........A..G...--.....--- 860 0 839 ..---..------T..A..A.AG.....T..A...------..T.....-.-----...A....G..A.....-A.-.......G..--- 902 0 284 ............ 295 E A K K Q A E A E A A K A A A E A Q K K A E A A A A K K A Q Query_1 789 GGCGAAGAAACAGGCGGAAGCTGAAGCGGCGAAAGCTGCGGCCGAGGCGCAGAAGAAAGCGGAAGCGGCGGCTGCGAAGAAAGCGCAACA 878 0 588 .......................................................................................... 677 0 343 .............AA...AAG...CA.......C.G..T....AA........C...---..------ 401 0 861 A..TG.T...GC...CA....G.C...A..A.....C.....---....A...---------................AG..... 933 0 340 .. 341 0 903 ..........GC..... 919 0 281 ............ 292 Q E A E K K A Q Q E A A K Q A A A E K A A A E K A A Query_1 879 GGAAGCGGAGAAAAAAGCCCAGCAGGAAGC---GGCTAA------GCAGGCGGCAGCTGAAAAAGCGGCTgccga---aaaagccgctga 956 0 678 ..............................---......------..............................---............ 755 0 402 C.CC....C.GC.......A.AGC...C..ACA...A..AGAAGC.....AA..C....CC........C.....GGCG.....GAAG.C 491 0 342 ........C....G....GA..G..C.-..---A.--..------......------....G...........A.---GGCC.....G.C 410 E K A A A Q K A A A E K A A A E K A A A A E K A A Query_1 957 g---------aaagccgccgcgca---aaaagcggccgctgagaaggccgccgccgagaaagccgcagccgctgaaaaagCG---GCGG- 1030 0 756 .---------..............---.......................................................---....- 829 0 492 .---------G.T...CAG...------......---..A..AC....G..G...A..GCG..T..T.A...-........A---.---- 555 0 411 .GCAAAAGCC.....G.A...A..GGC.....AA..-..A.-....-....T...---.....G..C....AG.CG......AAG....A 494 A A K A A A A E K A A A D K A A K A A A A Query_1 1031 ----CAG-CGAAAGCGGC------TGCAGCAGAGAAG--GCTGC---AGCT-----GATaaagc-ggccaa--agcggcagcagca---- 1092 0 830 ----...-..........------.............--.....---....-----........-......--.............---- 891 0 556 ------.-.CG....C..------......-..-...CC.....CGAG..G-----A.G...CA-...GG.--...T.A...G..G---- 618 0 326 ......------.....--.C.C..-----.A---...G-----.CG....A-A..G..GG...A....A....GGCT 381 0 495 TGCC...G..........AGAACAG..G..G.CC...--..G..---T...GACGC..A.....A....G.--...C..T.....G---- 573 K A A A A K K A A A A K E A D G V D N L L G D L S Query_1 1093 aaagccgcggc---ggcgaaga---------aagcggcggctgcgaaag---aagcggacggcgTTGACAACCTGCTCGGCGATCTGAGT 1167 0 892 ...........---........---------..................---...................................... 966 0 619 .....T.....CGA....C...AGAAAGCGG................GA---..... 672 0 382 G...AA.....---T..C...G---------CC..C.....G..A....CCA......... 430 0 574 ........T..CGA........---------..CA..... 604 S G K N A P K T G G G A K G N N A S A A G S G N T K N S A S Query_1 1168 TCTGGTAAGAATGCGCCTAAAACAGGCGGTGGGGCCAAAGGAAACAATGCCTCCGCTGCCGGGAGTGGTAATACTAAAAACAGTGCCTCA 1257 0 967 .......................................................................................... 1056 G A D I N N Y A G Q I K S A I E S K F Y D A S S Y A G K T C Query_1 1258 GGGGCTGATATCAACAACTATGCCGGACAGATAAAATCGGCGATTGAAAGTAAGTTTTATGACGCATCGTCCTATGCGGGCAAAACATGT 1347 0 1057 .......................................................................................... 1146 T L R I K L A P D G L L L N I Q S E G G D P A L C Q A A L A Query_1 1348 ACCTTGCGTATCAAACTTGCTCCTGACGGCCTGTTGTTAAATATACAGTCCGAAGGTGGTGATCCTGCTCTGTGCCAGGCCGCTCTTGCC 1437 0 1147 .......................................................................................... 1236 A A R Q A K F P K P P S Q A V Y E V F K N A P L D F K P Q Query_1 1438 GCAGCCCGACAGGCTAAGTTTCCGAAACCACCTAGCCAGGCAGTATATGAAGTCTTCAAAAATGCGCCACTGGACTTCAAACCTCAG 1524 0 1237 ....................................................................................... 1323 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2206344 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 1,333 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96885.103 (IDP96885.32-441.P(441)..pMCSG68)
SSFDEHLDASAGGGGGSSIDAVMVDPGAVVNNYNRQQQQQASARRAAEQREKQAQQQAEELREKQAAEQERLKQLEQERLQAQEAAKEAKEQQKQAEEAAAKAAAAAKAKADAQAKEAQEAAAKAAAEAKAKADAQAKAAEQAAAKAAADAKKQAEAAAAKAAAEAKKQAEAEAAKAAAEAQKKAEAAAAKKAQQEAEKKAQQEAAKQAAAEKAAAEKAAEKAAAQKAAAEKAAAEKAAAAEKAAAAKAAAAEKAAADKAAKAAAAKAAAAKKAAAAKEADGVDNLLGDLSSGKNAPKTGGGAKGNNASAAGSGNTKNSASGADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGLLLNIQSEGGDPALCQAALAAARQAKFPKPPSQAVYEVFKNAPLDFKPQ
AGTTCGTTTGACGAGCATCTGGATGCCTCTGCCGGCGGCGGCGGCGGATCGTCGATTGATGCCGTCATGGTCGATCCGGGGGCGGTGGTAAATAACTATAACCGTCAGCAACAGCAGCAGGCCAGCGCACGTCGCGCCGCTGAACAGCGTGAAAAACAGGCGCAGCAGCAGGCGGAAGAGTTACGTGAGAAACAGGCGGCGGAACAGGAACGGCTGAAACAGCTCGAACAGGAGCGGCTGCAGGCGCAGGAAGCGGCGAAAGAAGCGAAGGAGCAGCAGAAGCAGGCTGAAGAAGCGGCTGCCAAGGCCGCCGCGGCGGCAAAAGCCAAAGCGGACGCACAGGCAAAAGAAGCGCAGGAAGCCGCTGCCAAAGCGGCCGCCGAGGCGAAAGCGAAGGCGGATGCCCAGGCGAAAGCGGCAGAACAGGCGGCGGCCAAGGCGGCTGCTGACGCGAAAAAGCAGGCCGAAGCCGCTGCAGCGAAAGCCGCTGCCGAGGCGAAGAAACAGGCGGAAGCTGAAGCGGCGAAAGCTGCGGCCGAGGCGCAGAAGAAAGCGGAAGCGGCGGCTGCGAAGAAAGCGCAACAGGAAGCGGAGAAAAAAGCCCAGCAGGAAGCGGCTAAGCAGGCGGCAGCTGAAAAAGCGGCTGCCGAAAAAGCCGCTGAGAAAGCCGCCGCGCAAAAAGCGGCCGCTGAGAAGGCCGCCGCCGAGAAAGCCGCAGCCGCTGAAAAAGCGGCGGCAGCGAAAGCGGCTGCAGCAGAGAAGGCTGCAGCTGATAAAGCGGCCAAAGCGGCAGCAGCAAAAGCCGCGGCGGCGAAGAAAGCGGCGGCTGCGAAAGAAGCGGACGGCGTTGACAACCTGCTCGGCGATCTGAGTTCTGGTAAGAATGCGCCTAAAACAGGCGGTGGGGCCAAAGGAAACAATGCCTCCGCTGCCGGGAGTGGTAATACTAAAAACAGTGCCTCAGGGGCTGATATCAACAACTATGCCGGACAGATAAAATCGGCGATTGAAAGTAAGTTTTATGACGCATCGTCCTATGCGGGCAAAACATGTACCTTGCGTATCAAACTTGCTCCTGACGGCCTGTTGTTAAATATACAGTCCGAAGGTGGTGATCCTGCTCTGTGCCAGGCCGCTCTTGCCGCAGCCCGACAGGCTAAGTTTCCGAAACCACCTAGCCAGGCAGTATATGAAGTCTTCAAAAATGCGCCACTGGACTTCAAACCTCAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,649 total letters Query= IDP96885.103 Length=1635 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2219 0.0 2_08A_A2_IDP96885.103_T7term.seq 1900 0.0 1_02A_A2_IDP96885.103_T7S.seq 1853 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 60 CCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGA-GATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGT 148 1 8 ...........-.........................A...................---.............................. 93 S H P Q F E K G T E N L Y F Q S N A S S F D E H L D A S A G Query_1 149 CTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCAGTTCGTTTGACGAGCATCTGGATGCCTCTGCCGGCG 238 0 1 ..................................... 37 1 94 .......................................................................................... 183 G G G G S S I D A V M V D P G A V V N N Y N R Q Q Q Q Q A S Query_1 239 GCGGCGGCGGATCGTCGATTGATGCCGTCATGGTCGATCCGGGGGCGGTGGTAAATAACTATAACCGTCAGCAACAGCAGCAGGCCAGCG 328 0 38 .......................................................................................... 127 1 184 .......................................................................................... 273 A R R A A E Q R E K Q A Q Q Q A E E L R E K Q A A E Q E R L Query_1 329 CACGTCGCGCCGCTGAACAGCGTGAAAAACAGGCGCAGCAGCAGGCGGAAGAGTTACGTGAGAAACAGGCGGCGGAACAGGAACGGCTGA 418 0 128 .......................................................................................... 217 0 422 ............ 433 0 500 ............ 511 2 1172 .........NN...N......NN...-.N....... 1138 2 865 ............ 854 2 943 ............ 932 1 274 .......................................................................................... 363 1 568 ............ 579 1 646 ............ 657 K Q L E Q E R L Q A Q E A A K E A K E Q Q K Q Query_1 419 AACAGCTCGAACAGGAGCGGCTGCAG--GCGCAG---GAAGCGGCG-----AAAGA-AGCGAAGGAGCA-GCAG--AAGCAG--GC---- 488 0 218 ..........................--......---.........-----.....-............-....--......--..---- 287 0 517 .........-----....C-T...GCC...GC-....--...A.A--..---- 554 0 744 ...........CA.A.A..GCT.C...T.AT-----....C-G..C..--...G-....--C....--------- 798 0 583 ...---.......A.-----...A.-...CC..C..G.A..G.CT......--..GGCA 630 0 826 ..T...-----.....-.... 840 0 606 ....---........ 617 2 1137 .-.....NN.-.N.A....N......--......---N.N-.....-----N....T............-....--......---.---- 1071 2 848 .........-----....C-T...GCC...GC-....--...A.A--..---- 811 2 759 ....---........T-----..GC.-G...--.C...T-.A.A--....G.CT..---- 718 2 621 ...........CA.A.A..GCT.C...T.AT-----....C-G..C..--...G-....--C....--------- 567 2 782 ...---.......A.-----...A.-...CC..C..G.A..G.CT......--..GGCA 735 2 539 ..T...-----.....-.... 525 1 364 ..........................--......---.........-----.....-............-....--......--..---- 433 1 663 .........-----....C-T...GCC...GC-....--...A.A--..---- 700 1 729 ...---.......A.-----...A.-...CC..C..G.A..G.CT......--..GGCA 776 1 752 ....---........T-----..GC.-G...--.C...T-.A.A--....G.CT..---- 793 1 945 ...C..G..-.--...A..---A........CTGCG.....-.... 983 A E E A A A K A A A A A K A K A D A Q A K E A Query_1 489 --TGAAGAAGCGGCTGCCAAG---GC------CGCC---------GCGGCGGCAAAAGCCAAAGCGGACGCACAGGCAAAAGAAG-CGC- 556 0 288 --...................---..------....---------........................................-...- 355 0 555 --G....CG........G...AAA..GCAACAG.AA---------....A.AA.......C.GCA...A..---...T..-------..- 622 0 799 ---A...CC.....G..G...---AA------A..G---------.....T..G....---......... 844 0 631 GC....A...........G.A---AA------A...---------..T.A.---......---..C.-....-----.....CG.C...T 690 0 685 ..C....AG...---..------....---------.. 704 0 320 ..------....---------........ 307 0 802 ...---------........G..---G.......CG..---T..G.......-.. 840 0 812 ..------....---------..... 802 2 1070 --...G--.......N....N---NN------N--.---------.......N...-.............NNN.......-....-...- 1009 2 810 --G....CG........G...---AA------A..GCAACAGGAA....A.AA.......C.GCA...A..---...T..-------..- 743 2 717 --C...A....C....AG..A---..------....---------..----....-----.......C...------------T.-A.A- 671 2 566 ---A...CC.....G..G...---AA------A..G---------.....T..G....---......... 521 2 734 GC....A...........G.A---AA------A...---------..T.A.---......---..C.-....-----.....CG.C...T 675 2 680 ..C....AG...---..------....---------.. 661 2 563 ...---------........G..---G.......CG..---T..G.......-.. 525 2 553 ..------....---------..... 563 1 434 --...................---..------....---------........................................-...- 501 1 701 --G....CG........G...---AA------A..----------------....C..G--.......------.AA.....CCC-A..- 753 1 777 GC....A...........G.A---AA------A...---------..T.A.---......---..C.-....-----.....CG.C...T 836 1 794 --C...A....C....AG..A---..------....---------... 821 1 831 ..C....AG...---..------....---------.. 850 1 466 ..------....---------........ 453 1 946 ...---------........G..---G.......-...---T..G.......-.. 983 1 956 ..------....---------..... 946 Q E A A A K A A A E A K A K A D A Q A K A A E Q A Query_1 557 -AGGAAGCCGCTGCC---AAAGCGGCCGCCGAGGCGAAAGCGAAGGCGGAT---GCCCAGGCG---AAAGCG---GCAG---AACAGGCG 630 0 356 -..............---.................................---.........---......---....---........ 429 0 623 -...CG..A....AA---........T.....---A.....CGCT.A.A.A---...GCC...CAA......GCC..T.---.GA....C 699 0 691 G..A.G.....C...GAG.....C..A....CT.AA.....---.....--------..-...---......---..---------T..A 753 0 778 ..G...---........A.. 794 0 191 ........ 198 2 1008 -..............---....................-............---.........---......---....---........ 936 2 742 -...CG..A....AA---........T.....A---.....CGCT.A.A.A---...GCC...CAA......---..C.CTG.GA....C 666 2 670 -...CC.....C.AG---.....C..A....CT.AA.....---.....--------..-...---......---..---------T..A 612 2 674 G..A.G.....C...GAG.....C..A....CT.AA.....---.....--------..-...---......---..---------T..A 612 2 587 ..G...---........A.. 571 1 502 -..............---.................................---.........---......---....---........ 575 1 754 -.......G...AAG---C.G.....A..T..A---......GCT..C..AAAA...GCT.A.---.....C---..C.CGC..A.A... 830 1 837 G..A.G.....C...GAG.....C..A....CT.AA.....---.....--------..-...---.....T---..---------T..A 899 1 923 ..G...---........A.. 939 1 337 ........ 344 A A K A A A D A K K Q A E A A A A K A A A E A K K Q Query_1 631 GCGGCCAAGGCGGCTGCTGACGC-GAAAAA-GCAG-----GCCGAA---GCCGCTGCAGC-GA-AAGCCGCTGCCGAGGCGAAGAAACAG 708 0 430 .......................-......-....-----......---...........-..-.......................... 507 0 700 ..C...G..AAA..C..A.C...T......-..G.CGGCA..GA..---..G........A..G...--.....---A..TG.T...GC. 780 0 754 ..A.AG.....T..A...------..T...-..-.-----...A..---..G..A.....-A.-.......G..---..........GC. 822 0 188 ........ 195 0 199 .... 202 2 935 .......................-......-....-----......---...........-..-.......................... 858 2 665 ..C...G..AAA..C..A.C...T......-..G.CGGCA..GA..---..G........A..G...--.....A.CT.---.T...GC. 585 2 611 ..A.AG.....T..A...------..T...-..-.-----...A..---..G..A.....-A.-.......G..---..........GC. 543 2 611 ..A.AG.....T..A...------..T...-..-.-----...A..---..G..A.....-A.-.......G..---..........GC. 543 1 576 .......................-......-....-----......---...........-..-.......................... 653 1 831 ..C..TG..AA...C..C.C..A-....GCC....CC---..T...AAA..G..G.....-..-....T.....A.CA.A....------ 908 1 900 ..A.AG.....T..A....-------.T..-..-.-----...A..---..G..A.....--.-.......G..---..........GC. 966 1 334 ........ 341 1 345 .... 348 A E A E A A K A A A E A Q K K A E A A A A K K A Q Q Query_1 709 GCGGAAGCTGA--AGCGGCGAAAGCTGCGGC--------CGAGGCGCAGAAGAAAGCGGAAGCGGCGGCTGCGAAGAAAGCGCAACAGGA 788 0 508 ...........--..................--------................................................... 587 0 250 ..--...........AA...AA--------G...CA.......C.G..T....AA........C...---..------C.C 311 0 781 ..CA....G.C--...A..A.....C.....-----------....A...---------................AG..... 840 0 247 ..... 251 0 823 .... 826 0 196 .... 199 2 857 ...........--..................--------................................................... 778 2 584 ..CA....G.C--...A..A.....C.....-----------....A....---------...............AG..... 525 2 542 .... 539 2 542 .... 539 1 654 ...........--..................--------................................................... 733 1 909 ..T.C......TA......C.....G..A..AGCAAAGC..C....GC........... 967 1 396 ..--...........AA...AA--------G...CA.......C.G..T....AA........C...---..------C.C 457 1 393 ..... 397 1 967 ... 969 1 342 .... 345 E A E K K A Q Q E A A K Q A A A E K A A A E K A A E Query_1 789 AGCGGAGAAAAAAGCCCAGCAGGAAGC---GGCTAA------GCAGGCGGCAGCTGAAAAAGCGGCTgccga---aaaagccgctgag-- 864 0 588 ...........................---......------..............................---.............-- 663 0 312 C....C.GC.......A.AGC...C..ACA...A..AGAAGC.....AA..C....CC........C.....GGCG.....GAAG.C.-- 399 0 252 .....C....G....GA..G..C.-..---A.--..------......------....G...........A.---GGCC.....G.C.GC 320 2 777 ...........................---......------..............................---.............-- 702 1 734 ...........................---......------..............................---.............-- 809 1 458 C....C.GC.......A.AGC...C..ACA...A..AGAAGC.....AA..C....CC........C.....GGCG.....GAAG.C.-- 545 1 398 .....C....G....GA..G..C.-..---A.--..------......------....G...........A.---GGCC.....G.C.GC 466 K A A A Q K A A A E K A A A E K A A A A E K A A Query_1 865 -------aaagccgccgcgca---aaaagcggccgctgagaaggccgccgccgagaaagccgcagccgctgaaaaagCG---GCGG---- 937 0 664 -------..............---.......................................................---....---- 736 0 400 -------G.T...CAG...------......---..A..AC....G..G...A..GCG..T..T.A...-........A---.------- 462 0 321 AAAAGCC.....G.A...A..GGC.....AA..-..A.-....-....T...---.....G..C....AG.CG......AAG....ATGC 404 2 701 -------..............---.......................................................---....---- 629 2 898 ....C---..T.---- 890 1 810 -------..............---.......................................................---....---- 882 1 546 -------G.T...CAG...------......---..A..AC....G..G...A..GCG..T..T.A...-........A---.------- 608 1 467 AAAAGCC.....G.A...A..GGC.....AA..-..A.-....-....T...---.....G..C....AG.CG......AAG....ATGC 550 A A K A A A A E K A A A D K A A K A A A A Query_1 938 -CAG-CGAAAGCGGC------TGCAGCAGAGA------AG--GCTGC---AGCT-----GATaaagc-ggccaa--agcggcagcagca- 999 0 737 -...-..........------...........------..--.....---....-----........-......--.............- 798 0 463 ---.-.CG....C..------......-..-.------..CC.....CGAG..G-----A.G...CA-...GG.--...T.A...G..G- 525 0 233 ......------.....--.C.C------..-----.A---...G-----.CG....A-A..G..GG...A....A....G 285 0 405 C...G..........AGAACAG..G..G.CC.------..--..G..---T...GACGC..A.....A....G.--...C..T.....G- 480 2 628 -...-..........------...........------..--.....---....-----........-......--.............- 567 2 889 -...-.......C..------...C.AG.C..AGAAAC..--..G.A---....-----.---....-...G..--...T..G..C.--- 827 2 956 .-..........AGAACAG..G..G.CC.------..--..G..---T...GACGC..A.....A....G.--...C..T.....G- 885 1 883 -...-.......T..------...........------..--.....---....-----...-....-......--............-- 942 1 609 ---.-.CG....C..------......-..-.------..CC.....CGAG..G-----A.G...CA-...GG.--...T.A...G..G- 671 1 379 ......------.....--.C.C------..-----.A---...G-----.CG....A-A..G..GG...A....A....G 431 1 551 C...G..........AGAACAG..G..G.CC.------..--..G..---T...GACGC..A.....A....G.--...C..T.....G- 626 K A A A A K K A A A A K E A D G V D N L L G D Query_1 1000 ---aaagccgcggc---ggcga-aga---------aagcggcggctgcgaaag---aagcggacggcgTTGACAACCTGCTCGGCGATCT 1070 0 799 ---...........---.....-...---------..................---.................................. 869 0 526 ---.....T.....CGA....C-...AGAAAGCGG................GA---..... 579 0 286 GCTG...AA.....---T..C.-..G---------CC..C.....G..A....CCA......... 337 0 481 ---........T..CGA.....-...---------..CA..... 511 2 566 ---...........---.....-...---------..................---.................................. 496 2 826 -----..G...A.A---A.AA.GC.G---------................GA---..... 786 2 884 ---........T..CGA.....-...---------..CA..... 854 1 943 ---...........---.....-...---------.......-..........---.................-................ 1011 1 672 ---.....T.....CGA....C-...AGAAAGCGG................GA---..... 725 1 432 GCTG...AA.....---T..C.-..G---------CC..C.....G..A....CCA......... 483 1 627 ---........T..CGA.....-...---------..CA..... 657 L S S G K N A P K T G G G A K G N N A S A A G S G N T K N S Query_1 1071 GAGTTCTGGTAAGAATGCGCCTAAAACAGGCGGTGGGGCCAAAGGAAACAATGCCTCCGCTGCCGGGAGTGGTAATACTAAAAACAGTGC 1160 0 870 .......................................................................................... 959 2 495 .......................................................................................... 406 1 1012 ..........-...TGC.........ACA...NN....-.NN.....--N......N.............-.N................. 1096 A S G A D I N N Y A G Q I K S A I E S K F Y D A S S Y A G K Query_1 1161 CTCAGGGGCTGATATCAACAACTATGCCGGACAGATAAAATCGGCGATTGAAAGTAAGTTTTATGACGCATCGTCCTATG-CGGGCAAAA 1249 0 960 ................................................................................-......... 1048 2 405 ................................................................................-......... 317 1 1097 .CTCNN..NN..AT...--C...........-NN.....--NC.N.....GN.....--N...N...NN...NN..N...C...N..... 1179 T C T L R I K L A P D G L L L N I Q S E G G D P A L C Q A A Query_1 1250 CATGTACCTTGCGTATCAAACTTGCTCCTGACGGCCTGTTGTTAAATATACAGTCCGAAGGTGGTGATCCTGCTCTGTGCCAGGCCGCTC 1339 0 1049 .......................................................................................... 1138 2 316 .......................................................................................... 227 1 1180 ..... 1184 L A A A R Q A K F P K P P S Q A V Y E V F K N A P L D F K P Query_1 1340 TTGCCGCAGCCCGACAGGCTAAGTTTCCGAAACCACCTAGCCAGGCAGTATATGAAGTCTTCAAAAATGCGCCACTGGACTTCAAACCTC 1429 0 1139 .......................................................................................... 1228 2 226 .......................................................................................... 137 Q * H W K W I T D P N S S S V D K L A A A L E H H H H H H * Query_1 1430 AGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAG 1519 0 1229 .. 1230 2 136 .............................-............................................................ 48 D P A A N K A R K E Query_1 1520 ATCCGGCTGCTAACAAAG--CCCGAAA-GGAAG 1549 2 47 ..................CC.......N..... 15 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5830019 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 3,649 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96886.105 (IDP96886.243-511.P(511)..pMCSG73)
MRKYLNVYTSEEERLRYAINQGYIVPYYQPLVNGKTGEIYGVEILARWQNSTTPSRSPAEFIPLAERTGLIIPLTRSLMAQVNAQMRPLFSKLPDGFHIGLNISASHINAPTFIDDCLHFQHGFEGKAVKLMLEITEQEPLLLNGVVVDKLNTLHNRGFSIALDDFGTGYSGLSYLHELVFDYIKIDQSFVARVTGEAPSSKLLDCVIEMARTLSLRIIAEGVETQAQLEYLNRQNIHLLQDYYFWKPMPYVALVMLLLSKPKARIIEQ
ATGCGTAAATATCTCAATGTTTATACCTCTGAAGAGGAAAGACTGCGCTACGCCATCAATCAGGGCTATATCGTTCCCTACTATCAGCCGCTGGTGAATGGGAAAACCGGAGAGATATACGGCGTGGAAATTCTCGCCCGCTGGCAAAACAGCACGACGCCATCGCGCTCGCCAGCGGAATTTATTCCGCTGGCCGAACGTACCGGCTTAATTATTCCCCTAACCCGCAGCCTGATGGCGCAGGTCAACGCGCAGATGAGACCACTCTTTAGTAAACTACCGGATGGGTTTCATATTGGCCTCAATATCAGCGCGTCACACATTAACGCCCCAACGTTTATCGATGATTGCCTGCACTTTCAGCACGGTTTTGAGGGTAAAGCGGTGAAGCTGATGCTGGAGATCACTGAACAGGAACCGCTGTTGCTCAACGGGGTGGTTGTCGACAAACTCAATACATTGCACAATCGCGGTTTTTCCATCGCCCTGGACGACTTTGGCACCGGCTATTCCGGGCTTTCCTATCTCCACGAGCTGGTTTTCGACTATATCAAAATCGATCAGAGCTTTGTCGCCCGCGTGACCGGGGAGGCGCCGTCAAGCAAACTGCTGGACTGCGTGATAGAGATGGCCCGGACGTTGTCACTGCGCATTATTGCCGAGGGCGTCGAAACCCAGGCCCAACTGGAATATCTTAATCGCCAGAATATTCATCTGCTGCAGGACTACTATTTCTGGAAGCCGATGCCCTACGTCGCGCTGGTGATGCTCCTGCTGAGCAAACCGAAGGCGCGGATTATCGAACAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,357 total letters Query= IDP96886.105 Length=1212 Score E Sequences producing significant alignments: (Bits) Value 2_10H_H4_IDP96886.105_T7_Term.seq 1553 0.0 1_04H_H4_IDP96886.105_v73F.seq 1548 0.0 0_wt 1456 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 2 928 ...........A........G....------....................... 881 2 946 .....C........... 930 2 612 ...........T.. 625 1 45 ...........A........G....------....................... 92 1 360 ...........T.. 347 1 29 ............ 40 0 265 ...........T.. 252 A M R K Y L N V Y T S E E E R L R Y A I N Q G Y I V P Y Y Query_1 199 GCCATGCGTAAATATCTCAATGTTTATACCTCTGAAGAGGAAA-GACTGCGCTACGCCATCAATCAGGGCTATATCGTTCCCTACTATCA 287 2 880 ...........................................A.............................................. 791 1 93 ...........................................-.............................................. 181 0 1 ........................................-.............................................. 86 Q P L V N G K T G E I Y G V E I L A R W Q N S T T P S R S P Query_1 288 GCCGCTGGTGAATGGGAAAACCGGAGAGATATACGGCGTGGAAATTCTCGCCCGCTGGCAAAACAGCACGACGCCATCGCGCTCGCCAGC 377 2 790 .......................................................................................... 701 2 683 ...... 688 1 182 .......................................................................................... 271 1 289 ...... 284 0 87 .......................................................................................... 176 0 194 ...... 189 A E F I P L A E R T G L I I P L T R S L M A Q V N A Q M R P Query_1 378 GGAATTTATTCCGCTGGCCGAACGTACCGGCTTAATTATTCCCCTAACCCGCAGCCTGATGGCGCAGGTCAACGCGCAGATGAGACCACT 467 2 700 .......................................................................................... 611 2 689 .....AA........... 706 1 272 .......................................................................................... 361 1 283 .....AA........... 266 0 177 .......................................................................................... 266 0 188 .....AA........... 171 L F S K L P D G F H I G L N I S A S H I N A P T F I D D C L Query_1 468 CTTTAGTAAACTACCGGATGGGTTTCATATTGGCCTCAATATCAGCGCGTCACACATTAACGCCCCAACGTTTATCGATGATTGCCTGCA 557 2 610 .......................................................................................... 521 1 362 .......................................................................................... 451 0 267 .......................................................................................... 356 H F Q H G F E G K A V K L M L E I T E Q E P L L L N G V V V Query_1 558 CTTTCAGCACGGTTTTGAGGGTAAAGCGGTGAAGCTGATGCTGGAGATCACTGAACAGGAACCGCTGTTGCTCAACGGGGTGGTTGTCGA 647 2 520 .......................................................................................... 431 1 452 .......................................................................................... 541 0 357 .......................................................................................... 446 D K L N T L H N R G F S I A L D D F G T G Y S G L S Y L H E Query_1 648 CAAACTCAATACATTGCACAATCGCGGTTTTTCCATCGCCCTGGACGACTTTGGCACCGGCTATTCCGGGCTTTCCTATCTCCACGAGCT 737 2 430 .......................................................................................... 341 1 542 .......................................................................................... 631 0 447 .......................................................................................... 536 L V F D Y I K I D Q S F V A R V T G E A P S S K L L D C V I Query_1 738 GGTTTTCGACTATATCAAAATCGATCAGAGCTTTGTCGCCCGCGTGACCGGGGAGGCGCCGTCAAGCAAACTGCTGGACTGCGTGATAGA 827 2 340 .......................................................................................... 251 1 632 .......................................................................................... 721 0 537 .......................................................................................... 626 E M A R T L S L R I I A E G V E T Q A Q L E Y L N R Q N I H Query_1 828 GATGGCCCGGACGTTGTCACTGCGCATTATTGCCGAGGGCGTCGAAACCCAGGCCCAACTGGAATATCTTAATCGCCAGAATA-TTCATC 916 2 250 ...................................................................................-...... 162 1 722 ...................................................................................T...... 811 0 627 ...................................................................................-...... 715 L L Q D Y Y F W K P M P Y V A L V M L L L S K P K A R I I E Query_1 917 TGCTGCAGGACTACTA-TTTCTGGAAGCCGATGCCCTACGTCGCGCTGGTGATGCTCCTGCTGAGCAAACCGAAGGCGCGGATTATCGAA 1005 2 161 ................-......................................................................... 73 1 812 ................T......................................................................... 901 0 716 ................-......................................................................... 804 Q * H W K W I T D P N S S S V D K L A A A L E H H H H H H * Query_1 1006 CAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGA 1095 2 72 .......................--..... 45 1 902 .......................--..... 929 0 805 ... 807 D P A A N K A R K E A E L A A A T A E Q * L A * P L G A Query_1 1096 GATCCGGCTGCTAAC-AAAGCCCGAAAGGAAGCTGAGTTGG-CTGCTGCCACCGC-TGAGCAATAAC-TAGCATAACCCCTTGGGGCCTC 1181 2 49 ..............A....... 28 1 925 ..............-.........................G.............N...........N...N.................. 1012 S K R V L R G F L L K Query_1 1182 TAAACGGGTCTTGAGGGGTTTTTTGC-TGAAA 1212 1 1013 .....N....................T..... 1044 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3957564 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 3,357 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96892.103 (IDP96892.26-658.P(658)..pMCSG73)
AALQQDATPATREANQALYRKLPFADKTDFNNAHQGFIAPLPPAMLKGAQGNIIWDPAKYDFVKEGEKAPDTVNPSLWRQSQLLNIGGLFKVTDGVYQIRNLDLSNMTIIEGKTGITVIDPLLSAEPAKEALALYFAHRPKKPVVAVLFTHSHVDHYGGIRGVVDEADVKAGKVKIYAPAGFMEEAVSENIMAGTAMSRRASYMYGNLLKPDAKGQVGAGLGTTTSAGTVTLIPPTHYITHTGQQEVIDGLTYDFMMAPGSEAPSEMLWYVKEKKMIEAAEDVTHTLHNTYSLRGAKIRDPLAWSKYINAAIDRWGNEAEVIIAQHHWPTWGNDNIVKLMKGQRDMYRYINDQTLRMANLGMTRDEIAANFKLPDSLEKQWSSRGYYGSVSHDVKATYVFYLGWFDGNPATLDELPPEQAAKKFVEYMGGADAIMQKAKADYQQGNYRWVAQVTSKIVFADPDNQQARDLEADALEQLGYQAEAGTWRNFYLTGAQELRNGVQKLPTPNTASPDTVRAMTPEMFFDYLGVHINGEKAGAAKAVFNIDLGKDGGKYKLELENGVLNHTANAVADNADASISLSRDTLNKIILKQETLKQAEAQGKVKISGNGAKLDEMLSYMDTFAFWFNIVTP
GCCGCGCTACAACAGGATGCCACACCGGCCACGCGTGAGGCGAATCAGGCGCTATACCGCAAACTGCCGTTCGCGGATAAAACCGACTTTAATAACGCCCATCAGGGTTTTATCGCCCCCCTGCCGCCAGCGATGCTCAAAGGCGCACAGGGAAATATTATCTGGGATCCCGCCAAATATGATTTCGTCAAAGAAGGCGAAAAGGCGCCGGACACCGTCAATCCCAGCCTGTGGCGACAGTCACAATTGCTCAATATCGGCGGCTTGTTCAAAGTTACCGACGGCGTTTACCAGATCCGTAATCTCGATCTTTCCAATATGACCATTATCGAAGGCAAAACCGGGATTACGGTCATCGACCCCTTGCTGAGCGCCGAACCGGCCAAAGAGGCTCTGGCGCTCTATTTTGCCCACCGGCCGAAAAAACCGGTGGTGGCGGTGCTGTTTACCCACAGCCATGTGGACCACTACGGCGGTATCCGTGGCGTAGTCGATGAAGCTGACGTGAAGGCCGGTAAAGTGAAGATTTACGCCCCTGCCGGCTTTATGGAGGAAGCGGTTTCCGAGAACATCATGGCCGGTACTGCGATGAGCCGCCGCGCCAGCTACATGTACGGCAACCTGCTGAAGCCGGACGCTAAGGGACAGGTCGGCGCCGGTCTCGGCACCACCACCTCGGCGGGTACCGTTACGCTGATCCCGCCGACCCACTACATTACCCATACCGGTCAGCAGGAAGTGATCGACGGTCTGACCTATGACTTTATGATGGCGCCGGGCTCGGAAGCGCCGTCGGAAATGTTGTGGTATGTCAAAGAGAAGAAAATGATCGAGGCGGCAGAGGATGTGACCCATACCCTGCACAACACTTACTCTCTGCGCGGCGCCAAAATCCGCGATCCGCTGGCGTGGTCGAAATACATCAACGCCGCCATCGACCGCTGGGGCAACGAGGCGGAAGTGATTATCGCTCAGCACCACTGGCCGACCTGGGGGAACGACAATATCGTGAAGCTGATGAAAGGTCAGCGCGATATGTATCGCTATATCAACGACCAGACCCTGCGTATGGCGAACTTAGGCATGACCCGCGATGAGATCGCCGCCAACTTCAAGCTGCCGGATTCGCTGGAGAAACAGTGGTCCAGCCGCGGCTACTACGGTTCCGTTAGCCACGATGTGAAAGCGACCTACGTCTTCTATCTCGGCTGGTTTGATGGCAACCCGGCCACCCTCGACGAGCTGCCGCCAGAACAGGCGGCGAAGAAGTTCGTGGAATATATGGGCGGCGCCGATGCCATCATGCAGAAAGCCAAAGCCGACTACCAGCAGGGGAACTACCGCTGGGTGGCCCAGGTGACCAGCAAAATCGTCTTCGCCGATCCTGACAATCAGCAGGCGCGTGATCTTGAAGCCGACGCCCTGGAGCAGCTCGGATATCAGGCGGAAGCCGGGACCTGGCGCAACTTCTACCTCACCGGCGCTCAGGAGCTACGTAACGGCGTGCAGAAACTGCCGACGCCGAACACCGCCAGCCCGGATACCGTGCGGGCGATGACGCCAGAAATGTTCTTCGATTACCTCGGGGTGCATATCAACGGCGAAAAAGCCGGGGCGGCTAAAGCGGTCTTTAATATCGACCTGGGTAAAGACGGCGGCAAGTACAAACTGGAGCTGGAAAACGGCGTGCTTAACCATACCGCCAATGCGGTGGCGGACAATGCCGACGCCAGCATCTCGCTCAGCCGCGACACCCTGAATAAGATTATCCTCAAGCAGGAAACGCTGAAGCAGGCTGAAGCTCAGGGCAAGGTCAAGATTAGCGGTAATGGCGCTAAGCTCGACGAGATGCTGAGCTACATGGACACGTTTGCCTTCTGGTTCAATATCGTCACACCA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,373 total letters Query= IDP96892.103 Length=2304 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3425 0.0 1_05A_A5_IDP96892.103_v73F.seq 1920 0.0 2_11A_A5_IDP96892.103_T7_Term.seq 1885 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 1 42 ...........A........G....------....................... 89 1 24 .....C........... 40 A A A L Q Q D A T P A T R E A N Q A L Y R K L P F A D K T D Query_1 199 GCCGCCGCGCTACAACAGGATGCCACACCGGCCACGCGTGAGGCGAATCAGGCGCTATACCGCAAACTGCCGTTCGCGGATAAAACCGAC 288 0 1 ....................................................................................... 87 1 90 .......................................................................................... 179 F N N A H Q G F I A P L P P A M L K G A Q G N I I W D P A K Query_1 289 TTTAATAACGCCCATCAGGGTTTTATCGCCCCCCTGCCGCCAGCGATGCTCAAAGGCGCACAGGGAAATATTATCTGGGATCCCGCCAAA 378 0 88 .......................................................................................... 177 1 180 .......................................................................................... 269 Y D F V K E G E K A P D T V N P S L W R Q S Q L L N I G G L Query_1 379 TATGATTTCGTCAAAGAAGGCGAAAAGGCGCCGGACACCGTCAATCCCAGCCTGTGGCGACAGTCACAATTGCTCAATATCGGCGGCTTG 468 0 178 .......................................................................................... 267 1 270 .......................................................................................... 359 F K V T D G V Y Q I R N L D L S N M T I I E G K T G I T V I Query_1 469 TTCAAAGTTACCGACGGCGTTTACCAGATCCGTAATCTCGATCTTTCCAATATGACCATTATCGAAGGCAAAACCGGGATTACGGTCATC 558 0 268 .......................................................................................... 357 1 360 .......................................................................................... 449 D P L L S A E P A K E A L A L Y F A H R P K K P V V A V L F Query_1 559 GACCCCTTGCTGAGCGCCGAACCGGCCAAAGAGGCTCTGGCGCTCTATTTTGCCCACCGGCCGAAAAAACCGGTGGTGGCGGTGCTGTTT 648 0 358 .......................................................................................... 447 1 450 .......................................................................................... 539 T H S H V D H Y G G I R G V V D E A D V K A G K V K I Y A P Query_1 649 ACCCACAGCCATGTGGACCACTACGGCGGTATCCGTGGCGTAGTCGATGAAGCTGACGTGAAGGCCGGTAAAGTGAAGATTTACGCCCCT 738 0 448 .......................................................................................... 537 1 540 .......................................................................................... 629 A G F M E E A V S E N I M A G T A M S R R A S Y M Y G N L L Query_1 739 GCCGGCTTTATGGAGGAAGCGGTTTCCGAGAACATCATGGCCGGTACTGCGATGAGCCGCCGCGCCAGCTACATGTACGGCAACCTGCTG 828 0 538 .......................................................................................... 627 1 630 .......................................................................................... 719 K P D A K G Q V G A G L G T T T S A G T V T L I P P T H Y I Query_1 829 AAGCCGGACGCTAAGGGACAGGTCGGCGCCGGTCTCGGCACCACCACCTCGGCGGGTACCGTTACGCTGATCCCGCCGACCCACTACATT 918 0 628 .......................................................................................... 717 1 720 .......................................................................................... 809 T H T G Q Q E V I D G L T Y D F M M A P G S E A P S E M L W Query_1 919 ACCCATACCGGTCAGCAGGAAGTGATCGACGGTCTGACCTATGACTTTATGATGGCGCCGGGCTCGGAAGCGCCGTCGGAAATGTTGTGG 1008 0 718 .......................................................................................... 807 1 810 .......................................................................................... 899 2 1206 ...N.GN.................CNN.NN.---.N.N.C....TCNN....N.NNCN 1152 Y V K E K K M I E A A E D V T H T L H N T Y S L R G A K I Query_1 1009 TATGTC-AAAGAGAA--GAAAATGATCGAGGCGGCAGAGGATGTGACCCATACCCTGCACAACACTTACTCTCTGCGCGGCGCCAAAATC 1095 0 808 ......-........--......................................................................... 894 1 900 ......-........--...................................................................-..... 985 2 1151 NNN...C........ANN............-......N.-.....N..-..N..-......-....-..NNN....N.NN-......... 1069 R D P L A W S K Y I N A A I D R W G N E A E V I I A Q H H W Query_1 1096 CGCGATCCGCTGGCGTGGTCGAAATACATCAACGCCGCCATCGACCGCTGGGGCAACGAGGCGGAAGTGATTATCGCTCAGCACCACTGG 1185 0 895 .......................................................................................... 984 1 986 .................N.N....................................................................-. 1074 2 1068 -...............................N..........N.............................................. 980 P T W G N D N I V K L M K G Q R D M Y R Y I N D Q T L R Query_1 1186 CCGACCT-GGGGGAACGACAATATCG-TGAAGCTGATGAAAGGTCAGCGCGAT-ATGTA-TCGC-TATATCAACGACCAGACCCT-GCGT 1269 0 985 .......-..................-..........................-.....-....-....................-.... 1068 1 1075 NN.....G..................N..............-.N.........NN...NT....T.....TC....-........G.... 1162 2 979 .......-..................-..........................-.....-....-....................-.... 896 M A N L G M T R D E I A A N F K L P D S L E K Q W S S R G Y Query_1 1270 ATGGCGAACTTAGGCATGACCCGCGATGAGATCGCCGCCAACTTCAAGCTGCCGGATTCGCTGGAGAAACAGTGGTCCAGCCGCGGCTAC 1359 0 1069 .......................................................................................... 1158 1 1163 .....-N.....A............. 1187 2 895 .......................................................................................... 806 Y G S V S H D V K A T Y V F Y L G W F D G N P A T L D E L P Query_1 1360 TACGGTTCCGTTAGCCACGATGTGAAAGCGACCTACGTCTTCTATCTCGGCTGGTTTGATGGCAACCCGGCCACCCTCGACGAGCTGCCG 1449 0 1159 .......................................................................................... 1248 2 805 .......................................................................................... 716 P E Q A A K K F V E Y M G G A D A I M Q K A K A D Y Q Q G N Query_1 1450 CCAGAACAGGCGGCGAAGAAGTTCGTGGAATATATGGGCGGCGCCGATGCCATCATGCAGAAAGCCAAAGCCGACTACCAGCAGGGGAAC 1539 0 1249 .......................................................................................... 1338 2 715 .......................................................................................... 626 Y R W V A Q V T S K I V F A D P D N Q Q A R D L E A D A L E Query_1 1540 TACCGCTGGGTGGCCCAGGTGACCAGCAAAATCGTCTTCGCCGATCCTGACAATCAGCAGGCGCGTGATCTTGAAGCCGACGCCCTGGAG 1629 0 1339 .......................................................................................... 1428 2 625 .......................................................................................... 536 Q L G Y Q A E A G T W R N F Y L T G A Q E L R N G V Q K L P Query_1 1630 CAGCTCGGATATCAGGCGGAAGCCGGGACCTGGCGCAACTTCTACCTCACCGGCGCTCAGGAGCTACGTAACGGCGTGCAGAAACTGCCG 1719 0 1429 .......................................................................................... 1518 2 535 .......................................................................................... 446 T P N T A S P D T V R A M T P E M F F D Y L G V H I N G E K Query_1 1720 ACGCCGAACACCGCCAGCCCGGATACCGTGCGGGCGATGACGCCAGAAATGTTCTTCGATTACCTCGGGGTGCATATCAACGGCGAAAAA 1809 0 1519 .......................................................................................... 1608 2 445 .......................................................................................... 356 A G A A K A V F N I D L G K D G G K Y K L E L E N G V L N H Query_1 1810 GCCGGGGCGGCTAAAGCGGTCTTTAATATCGACCTGGGTAAAGACGGCGGCAAGTACAAACTGGAGCTGGAAAACGGCGTGCTTAACCAT 1899 0 1609 .......................................................................................... 1698 2 355 .......................................................................................... 266 T A N A V A D N A D A S I S L S R D T L N K I I L K Q E T L Query_1 1900 ACCGCCAATGCGGTGGCGGACAATGCCGACGCCAGCATCTCGCTCAGCCGCGACACCCTGAATAAGATTATCCTCAAGCAGGAAACGCTG 1989 0 1699 .......................................................................................... 1788 2 265 .......................................................................................... 176 K Q A E A Q G K V K I S G N G A K L D E M L S Y M D T F A F Query_1 1990 AAGCAGGCTGAAGCTCAGGGCAAGGTCAAGATTAGCGGTAATGGCGCTAAGCTCGACGAGATGCTGAGCTACATGGACACGTTTGCCTTC 2079 0 1789 .......................................................................................... 1878 2 175 .......................................................................................... 86 W F N I V T P * H W K W I T D P N S S S V D K L A A A L E H Query_1 2080 TGGTTCAATATCGTCACACCATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcac 2169 0 1879 ..................... 1899 2 85 .........................................--..... 40 H H H H H * D P A A N K A R K E Query_1 2170 caccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGC 2219 2 44 ..........................N.... 14 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 9884414 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 4,373 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96893.102 (IDP96893.1-88.F..pMCSG68)
MKKVLIVCGNGLGSSFIVEMNVKKILAEMNKEAEVAHTDLTSAKSETADLILSAKDIAEHLSSHSAKVVGLSNLLDNNKIKEILAENI
ATGAAAAAAGTGCTTATCGTGTGCGGTAATGGTTTGGGCAGCAGCTTCATCGTTGAAATGAATGTCAAAAAGATCCTTGCCGAAATGAATAAAGAAGCTGAAGTCGCACATACCGACCTGACGTCGGCAAAAAGCGAAACTGCCGATCTTATTCTCAGCGCTAAAGACATTGCAGAACATCTTAGCAGTCACTCTGCAAAGGTCGTTGGATTAAGTAATTTACTTGATAACAATAAAATTAAAGAAATTTTAGCAGAAAACATT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,739 total letters Query= IDP96893.102 Length=669 Score E Sequences producing significant alignments: (Bits) Value 1_02B_B2_IDP96893.102_T7S.seq 1095 0.0 2_08B_B2_IDP96893.102_T7term.seq 1045 0.0 0_wt 477 3e-138 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAA 90 1 5 .............-................... 36 2 597 .......................................................................................... 508 2 530 ...G...........T.T.C.C.C...........A... 568 E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E N Query_1 91 GAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAAC 180 1 37 .......................................................................................... 126 2 507 .......................................................................................... 418 L Y F Q S N A M K K V L I V C G N G L G S S F I V E M N V K Query_1 181 CTGTACTTCCAATCCAATGCCATGAAAAAAGTGCTTATCGTGTGCGGTAATGGTTTGGGCAGCAGCTTCATCGTTGAAATGAATGTCAAA 270 1 127 .......................................................................................... 216 2 417 .......................................................................................... 328 0 1 ..................................................................... 69 K I L A E M N K E A E V A H T D L T S A K S E T A D L I L S Query_1 271 AAGATCCTTGCCGAAATGAATAAAGAAGCTGAAGTCGCACATACCGACCTGACGTCGGCAAAAAGCGAAACTGCCGATCTTATTCTCAGC 360 1 217 .......................................................................................... 306 2 327 .......................................................................................... 238 0 70 .......................................................................................... 159 A K D I A E H L S S H S A K V V G L S N L L D N N K I K E I Query_1 361 GCTAAAGACATTGCAGAACATCTTAGCAGTCACTCTGCAAAGGTCGTTGGATTAAGTAATTTACTTGATAACAATAAAATTAAAGAAATT 450 1 307 .......................................................................................... 396 2 237 .......................................................................................... 148 0 160 .......................................................................................... 249 L A E N I * H W K W I T D P N S S S V D K L A A A L E H H H Query_1 451 TTAGCAGAAAACATTTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccac 540 1 397 .......................................................................................... 486 2 147 .......................................................................................... 58 0 250 ............... 264 H H H * D P A A N K A R K E A E L A A A T A E Q * L A * P Query_1 541 caccaccacTGAGATCCGGCTGCTAACAAAGCCCG-AAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCT 629 1 487 ...................................-...................................................... 575 2 57 ...................................A........ 14 L G A S K R V L R G F L L K Query_1 630 TGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAA 669 1 576 ........................................ 615 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1761876 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 2,739 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96894.103 (IDP96894.1-248.F..pMCSG73)
MLISTYQERQTRNREKIKQLLNFLKEETYSDFKTLMLLFGFRDHKSLYSLLAKTERMGLIQKHVLESRTMKISLWGITSDGLAVVLTPDDKIFPARFEPSKITGWTLEHHLDNQAARLILEKKGASGWINGDRATFISQYQVKHRPDGLLTLPDGKVIAIETERRLKTRARYQSIMASHLLARTRKQWIYVFYVVPDQQKKRALELLFDSIRHVIINHQRVPLEERHRLVFRIYTVDELKHLSLDRYT
ATGCTGATTTCGACATATCAAGAGCGCCAGACACGCAACAGAGAAAAAATAAAACAACTGCTGAACTTCCTGAAAGAAGAAACCTACAGCGATTTTAAAACGCTGATGCTGCTATTTGGTTTCCGGGATCATAAGTCGCTTTATTCGCTACTGGCAAAAACAGAGCGAATGGGGTTAATACAGAAGCATGTGCTTGAATCACGAACAATGAAGATTTCATTATGGGGGATCACCAGCGACGGGCTGGCGGTGGTGTTAACCCCGGATGATAAAATCTTTCCTGCTCGGTTTGAACCATCAAAAATCACCGGCTGGACGCTGGAACACCATCTTGATAATCAGGCCGCCAGGCTTATTCTGGAGAAGAAAGGCGCTTCAGGATGGATTAACGGTGACCGTGCCACCTTTATCAGCCAGTATCAGGTGAAACATCGCCCGGACGGGTTACTGACTCTGCCTGACGGTAAGGTTATTGCCATTGAAACCGAACGCCGCCTGAAAACCCGAGCCCGTTATCAGTCGATCATGGCAAGCCACCTGCTGGCCCGTACCCGCAAGCAGTGGATTTACGTTTTTTATGTTGTGCCCGATCAACAGAAGAAGCGTGCGCTTGAACTGCTGTTTGACAGCATCAGGCATGTCATCATCAACCATCAGCGCGTCCCGCTGGAAGAACGCCACCGTCTGGTGTTTCGCATTTATACCGTTGATGAACTGAAACACCTGTCATTAGACCGCTATACG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,221 total letters Query= IDP96894.103 Length=1149 Score E Sequences producing significant alignments: (Bits) Value 2_11B_B5_IDP96894.103_T7_Term.seq 1447 0.0 1_05B_B5_IDP96894.103_v73F.seq 1447 0.0 0_wt 1342 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 2 860 ...........A........G....------....................... 813 2 878 .....C........... 862 1 43 ...........A........G....------....................... 90 1 25 .....C........... 41 A M L I S T Y Q E R Q T R N R E K I K Q L L N F L K E E T Y Query_1 199 GCCATGCTGATTTCGACATATCAAGAGCGCCAGACACGCAACAGAGAAAAAATAAAACAACTGCTGAACTTCCTGAAAGAAGAAACCTAC 288 2 812 .......................................................................................... 723 1 91 .......................................................................................... 180 0 1 ....................................................................................... 87 S D F K T L M L L F G F R D H K S L Y S L L A K T E R M G L Query_1 289 AGCGATTTTAAAACGCTGATGCTGCTATTTGGTTTCCGGGATCATAAGTCGCTTTATTCGCTACTGGCAAAAACAGAGCGAATGGGGTTA 378 2 722 .......................................................................................... 633 1 181 .......................................................................................... 270 0 88 .......................................................................................... 177 I Q K H V L E S R T M K I S L W G I T S D G L A V V L T P D Query_1 379 ATACAGAAGCATGTGCTTGAATCACGAACAATGAAGATTTCATTATGGGGGATCACCAGCGACGGGCTGGCGGTGGTGTTAACCCCGGAT 468 2 632 .......................................................................................... 543 1 271 .......................................................................................... 360 0 178 .......................................................................................... 267 D K I F P A R F E P S K I T G W T L E H H L D N Q A A R L I Query_1 469 GATAAAATCTTTCCTGCTCGGTTTGAACCATCAAAAATCACCGGCTGGACGCTGGAACACCATCTTGATAATCAGGCCGCCAGGCTTATT 558 2 542 .......................................................................................... 453 1 361 .......................................................................................... 450 0 268 .......................................................................................... 357 L E K K G A S G W I N G D R A T F I S Q Y Q V K H R P D G L Query_1 559 CTGGAGAAGAAAGGCGCTTCAGGATGGATTAACGGTGACCGTGCCACCTTTATCAGCCAGTATCAGGTGAAACATCGCCCGGACGGGTTA 648 2 452 .......................................................................................... 363 1 451 .......................................................................................... 540 0 358 .......................................................................................... 447 L T L P D G K V I A I E T E R R L K T R A R Y Q S I M A S H Query_1 649 CTGACTCTGCCTGACGGTAAGGTTATTGCCATTGAAACCGAACGCCGCCTGAAAACCCGAGCCCGTTATCAGTCGATCATGGCAAGCCAC 738 2 362 .......................................................................................... 273 1 541 .......................................................................................... 630 0 448 .......................................................................................... 537 L L A R T R K Q W I Y V F Y V V P D Q Q K K R A L E L L F D Query_1 739 CTGCTGGCCCGTACCCGCAAGCAGTGGATTTACGTTTTTTATGTTGTGCCCGATCAACAGAAGAAGCGTGCGCTTGAACTGCTGTTTGAC 828 2 272 .......................................................................................... 183 1 631 .......................................................................................... 720 0 538 .......................................................................................... 627 S I R H V I I N H Q R V P L E E R H R L V F R I Y T V D E L Query_1 829 AGCATCAGGCATGTCATCATCAACCATCAGCGCGTCCCGCTGGAAGAACGCCACCGTCTGGTGTTTCGCATTTATACCGTTGATGAACTG 918 2 182 .......................................................................................... 93 2 1008 ........... 998 1 721 .......................................................................................... 810 0 628 .......................................................................................... 717 K H L S L D R Y T * H W K W I T D P N S S S V D K L A A A L Query_1 919 AAACACCTGTCATTAGACCGCTATACGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTC 1008 2 92 ...............................................--..... 41 1 811 ...............................................--..... 862 0 718 ........................... 744 E H H H H H H * D P A A N K A R K E A E L A A A T A E Q * Query_1 1009 GAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCC-GAAAGGAAGCTGAGTT-GGCTGCTGCCACCGCTGAGCAATAAC 1096 2 45 .....................G........ 16 1 858 .....................-................G.......................... 921 L A * P L G A S K R V L R G F L L K Query_1 1097 TAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAA 1149 1 922 ......-.N..............-............................. 972 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3595009 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 3,221 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96895.103 (IDP96895.1-211.F..pMCSG73)
MNFQEIHSYYKTLTIADFFADILVEGEEVILAASKNWRPETGYIYFCTEGSLSILMPDNGLSIGNSIEHMPIGLMERYCPLAKFEYTGSGPVTLRKITYAAFDRLFIENHPQRVEALAKVLVFMSIFTIDLHNERRQVTSYQTIRPMLYRYLYRQHTHEGENEGLALFIIRRTNLSRTHVFRVLADLKAGGYITMKRGKLISIDRPLPAEY
ATGAATTTCCAGGAAATACATAGTTATTATAAAACGTTAACCATAGCCGACTTTTTTGCTGATATCCTCGTGGAGGGTGAAGAGGTTATTCTCGCCGCCAGTAAAAATTGGCGTCCGGAGACAGGATATATTTATTTTTGTACCGAGGGTTCGTTATCAATATTAATGCCGGATAATGGATTGAGTATTGGTAATTCCATTGAGCATATGCCGATTGGCCTGATGGAGCGGTATTGCCCGCTGGCGAAATTTGAATATACAGGCAGTGGCCCGGTGACGCTGCGAAAAATCACTTACGCGGCCTTCGATCGTCTGTTTATTGAAAATCATCCGCAGCGCGTTGAGGCGCTGGCGAAAGTGCTGGTGTTCATGAGTATCTTTACCATCGACCTGCACAATGAACGCCGTCAGGTGACGAGCTATCAGACGATTCGCCCGATGCTATATCGCTATCTGTATCGGCAGCATACGCATGAGGGTGAAAATGAGGGACTGGCGTTATTTATCATCAGGCGTACCAATCTTTCGCGTACCCATGTTTTTCGCGTGCTGGCGGACTTAAAGGCGGGGGGATATATCACCATGAAACGTGGCAAGCTTATCTCTATCGATCGACCGTTGCCCGCAGAGTAC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,123 total letters Query= IDP96895.103 Length=1038 Score E Sequences producing significant alignments: (Bits) Value 2_11C_C5_IDP96895.103_T7_Term.seq 1247 0.0 1_05C_C5_IDP96895.103_v73F.seq 1247 0.0 0_wt 1142 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 2 751 ...........A........G....------....................... 704 2 769 .....C........... 753 1 43 ...........A........G....------....................... 90 1 27 ...C........... 41 A M N F Q E I H S Y Y K T L T I A D F F A D I L V E G E E V Query_1 199 GCCATGAATTTCCAGGAAATACATAGTTATTATAAAACGTTAACCATAGCCGACTTTTTTGCTGATATCCTCGTGGAGGGTGAAGAGGTT 288 2 703 .......................................................................................... 614 1 91 .......................................................................................... 180 1 850 ........... 860 0 1 ....................................................................................... 87 I L A A S K N W R P E T G Y I Y F C T E G S L S I L M P D N Query_1 289 ATTCTCGCCGCCAGTAAAAATTGGCGTCCGGAGACAGGATATATTTATTTTTGTACCGAGGGTTCGTTATCAATATTAATGCCGGATAAT 378 2 613 .......................................................................................... 524 1 181 .......................................................................................... 270 0 88 .......................................................................................... 177 G L S I G N S I E H M P I G L M E R Y C P L A K F E Y T G S Query_1 379 GGATTGAGTATTGGTAATTCCATTGAGCATATGCCGATTGGCCTGATGGAGCGGTATTGCCCGCTGGCGAAATTTGAATATACAGGCAGT 468 2 523 .......................................................................................... 434 2 354 ........... 344 1 271 .......................................................................................... 360 1 440 ........... 450 0 178 .......................................................................................... 267 0 347 ........... 357 G P V T L R K I T Y A A F D R L F I E N H P Q R V E A L A K Query_1 469 GGCCCGGTGACGCTGCGAAAAATCACTTACGCGGCCTTCGATCGTCTGTTTATTGAAAATCATCCGCAGCGCGTTGAGGCGCTGGCGAAA 558 2 433 .......................................................................................... 344 2 462 ........... 452 1 361 .......................................................................................... 450 1 332 ........... 342 0 268 .......................................................................................... 357 0 239 ........... 249 V L V F M S I F T I D L H N E R R Q V T S Y Q T I R P M L Y Query_1 559 GTGCTGGTGTTCATGAGTATCTTTACCATCGACCTGCACAATGAACGCCGTCAGGTGACGAGCTATCAGACGATTCGCCCGATGCTATAT 648 2 343 .......................................................................................... 254 1 451 .......................................................................................... 540 0 358 .......................................................................................... 447 R Y L Y R Q H T H E G E N E G L A L F I I R R T N L S R T H Query_1 649 CGCTATCTGTATCGGCAGCATACGCATGAGGGTGAAAATGAGGGACTGGCGTTATTTATCATCAGGCGTACCAATCTTTCGCGTACCCAT 738 2 253 .......................................................................................... 164 1 541 .......................................................................................... 630 0 448 .......................................................................................... 537 V F R V L A D L K A G G Y I T M K R G K L I S I D R P L P A Query_1 739 GTTTTTCGCGTGCTGGCGGACTTAAAGGCGGGGGGATATATCACCATGAAACGTGGCAAGCTTATCTCTATCGATCGACCGTTGCCCGCA 828 2 163 .......................................................................................... 74 1 631 .......................................................................................... 720 0 538 .......................................................................................... 627 E Y * H W K W I T D P N S S S V D K L A A A L E H H H H H H Query_1 829 GAGTACTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccac 918 2 73 ..........................--..... 43 1 721 ..........................--..... 751 0 628 ...... 633 * D P A A N K A R K E A E L A A A T A E Q * L A * P L G A Query_1 919 TGAGATCCGGCTGCT-AACAAAGCCC--GAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCC 1005 2 47 ...........T..........CNA....... 16 1 747 ...........-..........--.............................................................. 829 S K R V L R G F L L Query_1 1006 TCTAAACGGGTCTTGAGGGGTTTTTTGCTGAA 1037 2 649 ........... 639 1 830 ................................ 861 1 145 ........... 155 0 52 ........... 62 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3142650 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 3,123 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96897.103 (IDP96897.1-378.F..pMCSG73)
MAFTAGQPSTARALILNSDSRAMTAAAAADKVAALLRGAATFVPHWQVKKIDSTLRGNPGGELEAMMAAQGCRMAVVAPAYPAAGRHTRDGRCYVHGVPLDQTEFASDPKTPVSRAEISEIIAMQSRLPCLTLNAGQLPAALATAGEEKRVLIVDAWEDSHLDQVIDAVAPHARETLLVGSAGLCEALARRLRRSEQGPLLAVVGSMSEMAQRQVAALQVHSRVRVIEIDVEQAFSGSPKEEASRIAGALREGQHCVVTTRPNHAVRQGIEARCRERGLSRAAYGEHICAWLADVTAQAVAQSSPGALYLSGGDVAIAVAHALGATGFQIRGRVAECVPYGHFLGGRWSRPVMTKAGGFGTDTTLLHVVNFIEEKLSV
GTGGCCTTCACCGCCGGACAGCCATCAACCGCCCGGGCATTAATTCTCAACAGCGACAGCAGGGCGATGACCGCCGCCGCGGCGGCGGACAAGGTCGCCGCTCTGCTGCGCGGCGCGGCGACATTCGTCCCGCACTGGCAGGTAAAGAAGATCGACTCCACGCTGCGCGGTAATCCCGGCGGCGAACTGGAGGCAATGATGGCGGCGCAAGGCTGCCGGATGGCGGTGGTGGCCCCGGCTTATCCGGCAGCCGGGCGGCATACCCGGGACGGGCGCTGCTATGTGCATGGCGTGCCCCTCGATCAGACCGAATTTGCCAGCGATCCGAAAACGCCGGTGAGCCGGGCGGAGATTAGCGAAATTATCGCTATGCAGAGCCGTCTTCCCTGTCTGACCCTGAACGCTGGCCAGCTTCCGGCGGCATTAGCGACGGCGGGCGAGGAAAAGCGAGTGTTGATTGTCGACGCCTGGGAAGACAGCCATCTGGACCAGGTGATCGACGCCGTTGCGCCCCATGCGCGGGAGACCCTGCTGGTCGGCTCGGCGGGTCTCTGCGAGGCGCTGGCTCGCCGCCTGCGCCGCAGCGAGCAGGGCCCGCTGCTGGCGGTGGTCGGTTCGATGAGCGAGATGGCGCAGCGTCAGGTTGCTGCGCTGCAGGTCCATTCCCGAGTACGGGTGATTGAAATTGATGTCGAACAGGCCTTTAGCGGATCGCCAAAGGAGGAAGCTTCACGGATCGCCGGGGCGCTGCGCGAGGGCCAGCACTGCGTGGTGACCACCCGCCCGAATCACGCCGTCCGGCAAGGGATTGAAGCCCGGTGCCGTGAACGCGGCCTCAGCCGCGCCGCCTACGGGGAGCACATCTGCGCCTGGCTTGCCGACGTCACGGCGCAGGCGGTGGCGCAGTCTTCCCCTGGCGCGCTGTATCTTTCCGGCGGCGATGTGGCCATCGCCGTCGCGCACGCGCTCGGCGCCACAGGGTTTCAGATCCGCGGCCGGGTGGCGGAGTGCGTTCCATACGGGCATTTTCTCGGCGGCCGCTGGTCGCGGCCGGTGATGACCAAAGCCGGAGGATTCGGCACCGACACCACGCTGTTACATGTTGTGAATTTTATTGAGGAGAAACTGAGTGTC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,609 total letters Query= IDP96897.103 Length=1539 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2046 0.0 2_11D_D5_IDP96897.103_T7_Term.seq 1902 0.0 1_05D_D5_IDP96897.103_v73F.seq 1802 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 1 44 ...........A........G....------....................... 91 1 27 ....C........... 42 1 29 .............. 42 A V A F T A G Q P S T A R A L I L N S D S R A M T A A A A Query_1 199 GCCGTGGCCTTCACCGCCGGACAGCCATCAACCGCCCGGGCATTAATT-CTCAACAGCGACAGCAGGGCGATGACCGCCGCC-GCGG-CG 285 0 1 .............................................-.................................-....-.. 84 0 91 ..T........-....-.. 75 2 1169 ....C.N......T....N-..GNN.......N.....N.-......-....G.. 1118 2 1114 ........C....-.. 1128 1 92 ...A............................................-.................................-....-.. 178 1 185 ..T........-....-.. 169 A D K V A A L L R G A A T F V P H W Q V K K I D S T L R Query_1 286 GCGGA-CAAGGTCGCCGCTCTGCT-GCGCGG-CGCGGCGACATTCGTCCCGCACTGGCAGGTAAA-GAAGATCGACTCCACGCTGCGCGG 371 0 85 .....-..................-......-.................................-........................ 170 0 74 ....T-.. 68 2 1117 .....A...N..NN.-........T......N.................................A..........N.-........... 1030 2 1129 ... 1131 1 179 .....-..................-......-.................................-........................ 264 1 168 ....T-.. 162 G N P G G E L E A M M A A Q G C R M A V V A P A Y P A A G R Query_1 372 TAATCCCGGCGGCGAACTGGAGGCAATGATGGCGGCGCAAGGCTGCCGGAT-G-GCGGTGGTGGCCCCGGCTTATCCGGCAGCCGGGCGG 459 0 171 ...................................................-.-.................................... 258 0 252 ...........-AA..C.G..CCA..A.C..C-........... 211 2 1029 ...................................................-.-.................................... 942 2 948 ...........AA-..C.G..CCA..A.C..-C........... 989 1 265 ...................................................-.-.................................... 352 1 346 ...........-AA..C.G..CCA..A.C..C-........... 305 H T R D G R C Y V H G V P L D Q T E F A S D P K T P V S R A Query_1 460 CATACCCGGGACGGGCGCTGCTATGTGCATGGCGTGCCCCTCGATCAGACCGAATTTGCCAGCGATCCGAAAACGCCGGTGAGCCGGGCG 549 0 259 .......................................................................................... 348 2 941 ........................................................-................................. 853 1 353 .......................................................................................... 442 E I S E I I A M Q S R L P C L T L N A G Q L P A A L A T A G Query_1 550 GAGATTAGCGAAATTATCGCTATGCAGAGCCGTCTTCCCTGTCTGACCCTGAACGCTGGCCAGCTTCCGGCGGCATTAGCGACGGCGGGC 639 0 349 .......................................................................................... 438 2 852 .......................................................................................... 763 1 443 .......................................................................................... 532 E E K R V L I V D A W E D S H L D Q V I D A V A P H A R E T Query_1 640 GAGGAAAAGCGAGTGTTGATTGTCGACGCCTGGGAAGACAGCCATCTGGACCAGGTGATCGACGCCGTTGCGCCCCATGCGCGGGAGACC 729 0 439 .......................................................................................... 528 2 762 .......................................................................................... 673 1 533 .......................................................................................... 622 L L V G S A G L C E A L A R R L R R S E Q G P L L A V V G S Query_1 730 CTGCTGGTCGGCTCGGCGGGTCTCTGCGAGGCGCTGGCTCGCCGCCTGCGCCGCAGCGAGCAGGGCCCGCTGCTGGCGGTGGTCGGTTCG 819 0 529 .......................................................................................... 618 0 898 ............ 887 2 672 .......................................................................................... 583 2 303 ............ 314 1 623 .......................................................................................... 712 1 990 ............ 979 M S E M A Q R Q V A A L Q V H S R V R V I E I D V E Q A F S Query_1 820 ATGAGCGAGATGGCGCAGCGTCAGGTTGCTGCGCTGCAGGTCCATTCCCGAGTACGGGTGATTGAAATTGATGTCGAACAGGCCTTTAGC 909 0 619 .......................................................................................... 708 2 582 .......................................................................................... 493 1 713 .......................................................................................... 802 G S P K E E A S R I A G A L R E G Q H C V V T T R P N H A V Query_1 910 GGATCGCCAAAGGAGGAAGCTTCACGGATCGCCGGGGCGCTGCGCGAGGGCCAGCACTGCGTGGTGACCACCCGCCCGAATCACGCCGTC 999 0 709 .......................................................................................... 798 2 492 .......................................................................................... 403 1 803 .......................................................................................... 892 R Q G I E A R C R E R G L S R A A Y G E H I C A W L A D V T Query_1 1000 CGGCAAGGGATTGAAGCCCGGTGCCGTGAACGCGGCCTCAGCCGCGCCGCCTACGGGGAGCACATCTGCGCCTGGCTTGCCGACGTCACG 1089 0 799 .......................................................................................... 888 0 581 .. 580 2 402 .......................................................................................... 313 2 620 .. 621 1 893 ....-.................................................-................................... 980 1 675 .. 674 A Q A V A Q S S P G A L Y L S G G D V A I A V A H A L G A Query_1 1090 GCGCAGGCGGTGGCGCAGTCTTCCCCTGGCGCGCTGTAT--CTTTCCGGCGGCGATGTGGCCATCGCCGTCGCGCAC-GCGCTCGGCGCC 1176 0 889 .......................................--....................................-............ 975 0 579 .......... 570 2 312 .......................................--....................................-............ 226 2 622 .......... 631 1 981 ...........-.................NN....NG..TCT.....N.............................G.....TC..NNN 1069 1 673 .......... 664 T G F Q I R G R V A E C V P Y G H F L G G R W S R P V M T Query_1 1177 ACAGGGTTTCAGATCC-GCGGCCGGGTGGCGGAGTGCGTTCCATACGGGCATTTT-CTCGGCGGCCGCTGGTCGCGGCCGGTGATGACCA 1264 0 976 ................-......................................-.................................. 1063 2 225 ................-......................................-.................................. 138 1 1070 N.CA..A.........NN...NN.....N....NG.........TAC........C......-NNN.....N....NNNN.N.....A.T 1158 K A G G F G T D T T L L H V V N F I E E K L S V * H W K W I Query_1 1265 AAGCCGGAGGATTCGGCACCGACACCACGCTGTTACATGTTGTGAATTTTATTGAGGAGAAACTGAGTGTCTAACATTGGAAGTGGATAA 1354 0 1064 ....................................................................... 1134 2 137 .......................................................................................... 48 1 1159 .--G.N...A......N......-....... 1186 T D P N S S S V D K L A A A L E H H H H H H * D P A A N K A Query_1 1355 CGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCC 1444 2 47 .--..... 42 2 46 ..................... 26 R K E Query_1 1445 --GAAAGGAA 1452 2 25 CGA....... 16 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5423403 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 3,609 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96898.103 (IDP96898.1-1046.F..pMCSG73)
MDISNQKNHPQQIIPEPGQLVEVRRRQWIVVEVSSSQLPPPSSQQHLITLSSIDEDGLGELLEVIWEIEPGAQVIERAGLPSITGLDDSDTLEAFLDAVRWGAVTKADHRNFLQAPFRSGVSIEDFQLDPLVRAIDMARVNLLIADDVGLGKTIEAGLIIQEMLLRHRARTVLIVCPASLQEKWRVEMLEKFGLEFQIVDTAYIKRLRRERGIHANPWTSHPRLITSMDWAKSGEGLRSMRDVLPLKPSYPRKFDMLVIDEAHNIAPVGRANYAMESQRTRFIRSISPHFQHRLFLTATPHNGYTESFTSLLELLDDQRFARNVLPDEKLLNQVMIRRLKSDLVDSEGNPLYAQRKLKALSVEYSAQERSIHKKLDEYCHSREQGAEKASNTFGTTFVNQLLKKRLFSSPAAFASTLERHISTLMHGKRPNDKNAMADRILHKAILRLEEDYADDQEIENAQSEAVEEATHYTQPLTNEQKQILDVLRQWAQVAKNQVDSKAKAILDWINTNLKTNNQWNEYRVILFTEYRTTHQWMHEILASHGLAGERLAVLHGSMPQEEREKVKAAFQTSPKASAVRILLATDAASEGIDLQNYCNYLIHLEIPYNPNVMEQRNGRIDRHGQRQKEVVIWHPVDGVDKVNARIGGHGDDILRALRKLESMRADMGSVNPVIAPQMSGLIEGTIKDFDTRLAEARIAKTKRFIGVERELKERIAKLHERLLSARQELHLTPEHIQIAVNTALMLAGRTTLEPFELHDAPSGSVFRIPALSGTWARCLEGLRHPHTQEIRPVTFDHSVAKGRDDVVLVHLNHLLVQMCLRLLRAEIWARDDIKKLHRVTVRSVPDMLLESPAVAVVSRLVITGGNHHRLHEELTIAGGYLGDKNFRREDGVNKLQQWFEQAKPVTTEFSLLDAFRMRFERAQNSILQSVEARSKDRLQYLTNTLQTRKERELSDINNILNELEITIKSELAKDQQPMQFSLFSEDERAQIKRDNAALEARLARIPAEREQEVQTIELRYTNIDDRTFPVAVIFLVPESTIQRGAA
ATGGATATTTCGAATCAAAAAAATCATCCTCAACAAATTATTCCAGAGCCAGGGCAACTTGTTGAAGTTCGTCGACGCCAGTGGATCGTTGTTGAGGTTTCATCTTCCCAGTTACCACCGCCTTCGTCTCAGCAGCATCTCATAACTCTTTCATCCATAGATGAAGATGGTTTAGGAGAACTACTTGAAGTTATCTGGGAAATTGAACCTGGCGCTCAAGTAATTGAACGAGCTGGTTTACCTTCGATTACTGGCCTGGATGACTCTGACACTCTTGAGGCTTTTCTTGATGCAGTGCGTTGGGGAGCTGTCACTAAAGCTGACCATCGTAACTTCTTACAAGCACCTTTTCGTAGTGGCGTAAGCATTGAAGACTTCCAGCTTGATCCGTTGGTGCGTGCAATTGATATGGCCCGTGTCAACCTGCTTATCGCCGATGATGTTGGACTTGGTAAGACCATTGAAGCTGGGCTAATCATCCAGGAAATGTTACTTCGGCATCGAGCTCGCACAGTTTTGATTGTTTGCCCTGCGTCGCTGCAAGAGAAGTGGCGTGTTGAGATGTTAGAAAAATTCGGTCTTGAATTTCAAATCGTCGACACCGCTTATATTAAGCGATTAAGACGAGAACGTGGCATTCATGCCAATCCGTGGACATCGCATCCACGGTTAATCACTTCAATGGATTGGGCCAAAAGTGGGGAAGGTTTGCGTTCTATGCGTGACGTCCTTCCCTTAAAGCCTAGTTATCCACGAAAGTTTGACATGCTTGTCATTGATGAGGCACACAATATAGCCCCAGTGGGTAGAGCTAACTATGCAATGGAAAGCCAGCGTACTAGGTTTATTCGTTCGATAAGCCCACACTTCCAACACCGTTTGTTTTTGACAGCGACTCCACATAATGGCTATACCGAGTCTTTCACTTCGTTGCTTGAGTTGCTGGATGATCAACGTTTTGCCAGAAATGTACTTCCAGATGAGAAGTTGTTGAATCAGGTGATGATACGCCGCCTCAAAAGTGATCTGGTCGATTCAGAGGGCAATCCACTTTACGCTCAACGTAAGCTCAAAGCCTTATCCGTCGAATATTCGGCTCAAGAGCGTTCTATCCACAAAAAACTGGATGAATATTGCCATAGCCGGGAGCAAGGAGCAGAGAAAGCCAGCAATACTTTTGGGACGACCTTTGTCAACCAGCTACTTAAAAAAAGACTTTTTTCATCACCAGCAGCCTTTGCATCTACTCTTGAAAGGCACATATCAACTTTGATGCATGGCAAACGGCCTAATGATAAAAATGCCATGGCTGATCGTATCCTTCATAAAGCAATCCTTCGTCTAGAAGAAGATTACGCCGACGATCAAGAGATAGAGAATGCCCAATCTGAAGCGGTGGAAGAAGCAACACATTACACCCAACCGCTGACGAATGAACAGAAACAAATACTCGATGTATTGCGTCAATGGGCGCAAGTAGCTAAGAATCAGGTAGACTCTAAAGCCAAGGCAATACTTGATTGGATTAATACGAATCTTAAAACAAATAATCAGTGGAATGAGTACCGGGTAATTCTGTTTACTGAATATCGAACGACACATCAATGGATGCATGAGATTCTGGCCAGCCACGGACTTGCTGGTGAGCGCTTAGCTGTTTTACATGGTAGCATGCCGCAAGAAGAACGAGAAAAAGTTAAAGCCGCCTTCCAGACATCACCAAAGGCTTCTGCTGTTCGCATACTCCTCGCAACTGATGCCGCCTCAGAAGGCATCGACTTGCAAAACTATTGTAACTACCTTATCCATTTAGAAATTCCATATAACCCCAATGTAATGGAACAACGTAACGGTCGTATAGACCGCCACGGACAACGCCAAAAAGAAGTTGTAATTTGGCACCCAGTTGATGGTGTGGATAAGGTGAATGCTCGTATCGGTGGTCACGGTGACGATATCCTACGAGCATTACGCAAACTTGAATCAATGCGGGCTGACATGGGTAGCGTCAATCCAGTCATCGCACCTCAAATGTCAGGACTGATTGAAGGCACTATCAAAGATTTTGATACACGTCTGGCTGAAGCCAGAATTGCAAAAACGAAACGTTTTATTGGTGTTGAGCGCGAACTAAAAGAGAGAATTGCGAAGCTACATGAGCGACTCCTTTCAGCCAGACAGGAGCTTCATCTCACACCTGAGCATATACAAATAGCCGTCAATACAGCTCTCATGTTAGCTGGTCGTACAACTCTTGAACCTTTTGAGTTACACGATGCTCCCTCAGGTTCTGTATTCAGGATACCTGCTCTCTCAGGTACATGGGCACGTTGTCTTGAAGGACTTCGTCATCCCCATACACAGGAGATTCGTCCTGTCACTTTTGATCACAGCGTGGCGAAAGGACGTGATGATGTCGTACTCGTCCATTTAAATCACCTGTTAGTGCAGATGTGTCTACGTCTATTACGAGCAGAAATATGGGCGCGCGATGATATAAAAAAATTACATCGAGTTACGGTGCGCTCAGTACCAGACATGTTACTTGAAAGCCCTGCGGTCGCTGTTGTTTCACGATTGGTGATTACTGGAGGTAATCACCATCGACTTCATGAAGAATTGACAATAGCTGGTGGCTATCTTGGCGACAAAAATTTTAGGCGCGAGGATGGTGTTAATAAACTACAGCAATGGTTTGAGCAGGCGAAACCTGTTACTACTGAATTTTCGCTTTTGGATGCCTTTCGCATGCGTTTTGAGCGAGCACAAAACAGTATTCTCCAGTCCGTTGAAGCTAGATCAAAAGATCGCCTGCAATATCTGACTAATACCCTTCAGACCCGCAAAGAGCGTGAATTGTCTGATATCAATAACATACTCAATGAACTTGAAATAACTATCAAATCTGAGTTAGCTAAAGATCAACAACCTATGCAGTTTTCATTGTTTTCAGAAGATGAACGCGCTCAAATCAAGCGTGATAATGCAGCGCTCGAAGCCCGTCTCGCTCGCATTCCCGCAGAGCGGGAACAGGAAGTACAAACAATCGAACTTCGCTATACCAACATTGATGATCGCACATTCCCTGTCGCCGTCATTTTTCTCGTCCCAGAATCTACTATTCAGAGAGGTGCAGCA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,660 total letters Query= IDP96898.103 Length=3543 Score E Sequences producing significant alignments: (Bits) Value 0_wt 5660 0.0 2_11E_E5_IDP96898.103_T7_Term.seq 1950 0.0 1_05E_E5_IDP96898.103_v73F.seq 1820 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 1 42 ...........A........G....------....................... 89 1 24 .....C........... 40 A M D I S N Q K N H P Q Q I I P E P G Q L V E V R R R Q W I Query_1 199 GCCATGGATATTTCGAATCaaaaaaaTCATCCTCAACAAATTATTCCAGAGCCAGGGCAACTTGTTGAAGTTCGTCGACGCCAGTGGATC 288 0 1 ....................................................................................... 87 1 90 .......................................................................................... 179 V V E V S S S Q L P P P S S Q Q H L I T L S S I D E D G L G Query_1 289 GTTGTTGAGGTTTCATCTTCCCAGTTACCACCGCCTTCGTCTCAGCAGCATCTCATAACTCTTTCATCCATAGATGAAGATGGTTTAGGA 378 0 88 .......................................................................................... 177 1 180 .......................................................................................... 269 E L L E V I W E I E P G A Q V I E R A G L P S I T G L D D S Query_1 379 GAACTACTTGAAGTTATCTGGGAAATTGAACCTGGCGCTCAAGTAATTGAACGAGCTGGTTTACCTTCGATTACTGGCCTGGATGACTCT 468 0 178 .......................................................................................... 267 1 270 .......................................................................................... 359 D T L E A F L D A V R W G A V T K A D H R N F L Q A P F R S Query_1 469 GACACTCTTGAGGCTTTTCTTGATGCAGTGCGTTGGGGAGCTGTCACTAAAGCTGACCATCGTAACTTCTTACAAGCACCTTTTCGTAGT 558 0 268 .......................................................................................... 357 1 360 .......................................................................................... 449 G V S I E D F Q L D P L V R A I D M A R V N L L I A D D V G Query_1 559 GGCGTAAGCATTGAAGACTTCCAGCTTGATCCGTTGGTGCGTGCAATTGATATGGCCCGTGTCAACCTGCTTATCGCCGATGATGTTGGA 648 0 358 .......................................................................................... 447 1 450 .......................................................................................... 539 L G K T I E A G L I I Q E M L L R H R A R T V L I V C P A S Query_1 649 CTTGGTAAGACCATTGAAGCTGGGCTAATCATCCAGGAAATGTTACTTCGGCATCGAGCTCGCACAGTTTTGATTGTTTGCCCTGCGTCG 738 0 448 .......................................................................................... 537 1 540 .......................................................................................... 629 L Q E K W R V E M L E K F G L E F Q I V D T A Y I K R L R R Query_1 739 CTGCAAGAGAAGTGGCGTGTTGAGATGTTAGAAAAATTCGGTCTTGAATTTCAAATCGTCGACACCGCTTATATTAAGCGATTAAGACGA 828 0 538 .......................................................................................... 627 1 630 .......................................................................................... 719 E R G I H A N P W T S H P R L I T S M D W A K S G E G L R Query_1 829 GAACGTGGCATTCATGCCAATCCGTGGACATCGCATCCACGGTTAATCACTTC-AATGGATTGGGCCAAAAGTGGGGAAGG-TTTGCGTT 916 0 628 .....................................................-...........................-........ 715 1 720 .....................................................A...........................T........ 809 S M R D V L P L K P S Y P R K F D M L V I D E A H N I A P V Query_1 917 CTATGCGTGACGTCCTTCCCTTAAAGCCTAGTTATCCACGAAAGTTTGACATGCTTGTCATTGATGAGGCACACAATATAGCCCCAGT-G 1005 0 716 ........................................................................................-. 804 1 810 ........................................................................................G. 899 G R A N Y A M E S Q R T R F I R S I S P H F Q H R L F L T Query_1 1006 GGTAGAGCTAACTATGCAATGGAAAGCCAGCGTACTAGGTTTATTCGTTCGATAAGCCCACACTT-CCAACACCGTTTGTTTTTGACAGC 1094 0 805 .................................................................-........................ 893 1 900 ....................................................N............N........................ 989 A T P H N G Y T E S F T S L L E L L D D Q R F A R N V L P D Query_1 1095 GAC-TCCACATAATGGC-TATACCGAGTCTTTCACTTCGTTGCTTGAGTTGCTGGATGATCAACGTTTTGCCAGAAATGTACTTCCAGAT 1182 0 894 ...-.............-........................................................................ 981 1 990 ...T.............T...........C..N..........N...............................T...........NN. 1079 E K L L N Q V M I R R L K S D L V D S E G N P L Y A Q R Query_1 1183 G-AGAAGTTGTTGAATCAGGT-GATGATACGCCGCCTCAAAAGTGATCT--GGTCGATTCAGAGGGCAATCCACTTTACG-CTCAACGT- 1266 0 982 .-...................-...........................--.............................-........- 1065 1 1080 .N.N...........N.NNN.G..NN.....N......-..........TG.T....A.TNNN.......T.....NN..C..N....NT 1168 K L K A L S V E Y S A Q E R S I H K K L D E Y C H S R E Q Query_1 1267 -AAGC-TCAAAG-CCTTATCCGTCGAATATTCGGCTCAAGAGCGTTCTATCCACAAAAAACTGGATGAATATTGCCATAGCCGGGAGCAA 1353 0 1066 -....-......-............................................................................. 1152 1 1169 A....N......T..NN..T..GNCN.N.N...CN...... 1209 G A E K A S N T F G T T F V N Q L L K K R L F S S P A A F A Query_1 1354 GGAGCAGAGAAAGCCAGCAATACTTTTGGGACGACCTTTGTCAACCAGCTACTTaaaaaaaGACTTTTTTCATCACCAGCAGCCTTTGCA 1443 0 1153 .......................................................................................... 1242 S T L E R H I S T L M H G K R P N D K N A M A D R I L H K A Query_1 1444 TCTACTCTTGAAAGGCACATATCAACTTTGATGCATGGCAAACGGCCTAATGATAAAAATGCCATGGCTGATCGTATCCTTCATAAAGCA 1533 0 1243 .......................................................................................... 1332 I L R L E E D Y A D D Q E I E N A Q S E A V E E A T H Y T Q Query_1 1534 ATCCTTCGTCTAGAAGAAGATTACGCCGACGATCAAGAGATAGAGAATGCCCAATCTGAAGCGGTGGAAGAAGCAACACATTACACCCAA 1623 0 1333 .......................................................................................... 1422 P L T N E Q K Q I L D V L R Q W A Q V A K N Q V D S K A K A Query_1 1624 CCGCTGACGAATGAACAGAAACAAATACTCGATGTATTGCGTCAATGGGCGCAAGTAGCTAAGAATCAGGTAGACTCTAAAGCCAAGGCA 1713 0 1423 .......................................................................................... 1512 I L D W I N T N L K T N N Q W N E Y R V I L F T E Y R T T H Query_1 1714 ATACTTGATTGGATTAATACGAATCTTAAAACAAATAATCAGTGGAATGAGTACCGGGTAATTCTGTTTACTGAATATCGAACGACACAT 1803 0 1513 .......................................................................................... 1602 Q W M H E I L A S H G L A G E R L A V L H G S M P Q E E R E Query_1 1804 CAATGGATGCATGAGATTCTGGCCAGCCACGGACTTGCTGGTGAGCGCTTAGCTGTTTTACATGGTAGCATGCCGCAAGAAGAACGAGAA 1893 0 1603 .......................................................................................... 1692 K V K A A F Q T S P K A S A V R I L L A T D A A S E G I D L Query_1 1894 AAAGTTAAAGCCGCCTTCCAGACATCACCAAAGGCTTCTGCTGTTCGCATACTCCTCGCAACTGATGCCGCCTCAGAAGGCATCGACTTG 1983 0 1693 .......................................................................................... 1782 Q N Y C N Y L I H L E I P Y N P N V M E Q R N G R I D R H G Query_1 1984 CAAAACTATTGTAACTACCTTATCCATTTAGAAATTCCATATAACCCCAATGTAATGGAACAACGTAACGGTCGTATAGACCGCCACGGA 2073 0 1783 .......................................................................................... 1872 Q R Q K E V V I W H P V D G V D K V N A R I G G H G D D I L Query_1 2074 CAACGCCAAAAAGAAGTTGTAATTTGGCACCCAGTTGATGGTGTGGATAAGGTGAATGCTCGTATCGGTGGTCACGGTGACGATATCCTA 2163 0 1873 .......................................................................................... 1962 R A L R K L E S M R A D M G S V N P V I A P Q M S G L I Query_1 2164 CGAGCATTACGC--AAACTTGAATC--AATGCGGGCTGACATGGGTAGCGTCAATCCAGTCATCGCACCTCAAATGTCAGGACTG-ATTG 2248 0 1963 ............--...........--..........................................................-.... 2047 2 1237 .......NC..N.......TCA.............NN.NA-...N...NNN.....NNN..N...TNNNN-.........N.... 1155 E G T I K D F D T R L A E A R I A K T K R F I G V E R E L K Query_1 2249 AAGGCAC-TATCAAAGATTTTGATACACGTCTGGCTGAAGCCAGAATTGCAAAAACGAAACGTTTTATTGGTGTTGAGCGCGAACTAAAA 2337 0 2048 .......-.................................................................................. 2136 0 2179 ........... 2169 2 1154 N-....NN..........................N...-......-...........................................G 1068 E R I A K L H E R L L S A R Q E L H L T P E H I Q I A V N T Query_1 2338 GAGAGAATTGCGAAGCTACATGAGCGACTCCTTTCAGCCAGACAGGAGCTTCATCTCACACCTGAGCATATACAAATAGCCGTCAATACA 2427 0 2137 .......................................................................................... 2226 2 1067 ANA..............................CAG........................NTGA.......................... 978 A L M L A G R T T L E P F E L H D A P S G S V F R I P A L S Query_1 2428 GCTCTCATGTTAGCTGGTCGTACAACTCTTGAACCTTTTGAGTTACACGATGCTCCCTCAGGTTCTGTATTCAGGATACCTGCTCTCTCA 2517 0 2227 .......................................................................................... 2316 2 977 .......................................................................................... 888 G T W A R C L E G L R H P H T Q E I R P V T F D H S V A K G Query_1 2518 GGTACATGGGCACGTTGTCTTGAAGGACTTCGTCATCCCCATACACAGGAGATTCGTCCTGTCACTTTTGATCACAGCGTGGCGAAAGGA 2607 0 2317 .......................................................................................... 2406 2 887 .......................................................................................... 798 R D D V V L V H L N H L L V Q M C L R L L R A E I W A R D D Query_1 2608 CGTGATGATGTCGTACTCGTCCATTTAAATCACCTGTTAGTGCAGATGTGTCTACGTCTATTACGAGCAGAAATATGGGCGCGCGATGAT 2697 0 2407 .......................................................................................... 2496 2 797 .......................................................................................... 708 I K K L H R V T V R S V P D M L L E S P A V A V V S R L V I Query_1 2698 ATaaaaaaaTTACATCGAGTTACGGTGCGCTCAGTACCAGACATGTTACTTGAAAGCCCTGCGGTCGCTGTTGTTTCACGATTGGTGATT 2787 0 2497 .......................................................................................... 2586 2 707 .......................................................................................... 618 T G G N H H R L H E E L T I A G G Y L G D K N F R R E D G V Query_1 2788 ACTGGAGGTAATCACCATCGACTTCATGAAGAATTGACAATAGCTGGTGGCTATCTTGGCGACAAAAATTTTAGGCGCGAGGATGGTGTT 2877 0 2587 .......................................................................................... 2676 2 617 .......................................................................................... 528 N K L Q Q W F E Q A K P V T T E F S L L D A F R M R F E R A Query_1 2878 AATAAACTACAGCAATGGTTTGAGCAGGCGAAACCTGTTACTACTGAATTTTCGCTTTTGGATGCCTTTCGCATGCGTTTTGAGCGAGCA 2967 0 2677 .......................................................................................... 2766 2 527 .......................................................................................... 438 Q N S I L Q S V E A R S K D R L Q Y L T N T L Q T R K E R E Query_1 2968 CAAAACAGTATTCTCCAGTCCGTTGAAGCTAGATCAAAAGATCGCCTGCAATATCTGACTAATACCCTTCAGACCCGCAAAGAGCGTGAA 3057 0 2767 .......................................................................................... 2856 2 437 .......................................................................................... 348 L S D I N N I L N E L E I T I K S E L A K D Q Q P M Q F S L Query_1 3058 TTGTCTGATATCAATAACATACTCAATGAACTTGAAATAACTATCAAATCTGAGTTAGCTAAAGATCAACAACCTATGCAGTTTTCATTG 3147 0 2857 .......................................................................................... 2946 2 347 .......................................................................................... 258 F S E D E R A Q I K R D N A A L E A R L A R I P A E R E Q E Query_1 3148 TTTTCAGAAGATGAACGCGCTCAAATCAAGCGTGATAATGCAGCGCTCGAAGCCCGTCTCGCTCGCATTCCCGCAGAGCGGGAACAGGAA 3237 0 2947 .......................................................................................... 3036 2 257 .......................................................................................... 168 V Q T I E L R Y T N I D D R T F P V A V I F L V P E S T I Q Query_1 3238 GTACAAACAATCGAACTTCGCTATACCAACATTGATGATCGCACATTCCCTGTCGCCGTCATTTTTCTCGTCCCAGAATCTACTATTCAG 3327 0 3037 .......................................................................................... 3126 2 167 .......................................................................................... 78 R G A A * H W K W I T D P N S S S V D K L A A A L E H H H H Query_1 3328 AGAGGTGCAGCATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccac 3417 0 3127 ............ 3138 2 77 ................................--..... 41 H H * D P A A N K A Query_1 3418 caccacTGAGATCCGGCTGCTAACAAAGCCC 3448 2 45 ..................... 25 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 19761150 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 5,660 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96899.103 (IDP96899.1-345.F..pMCSG73)
MAFSPLFIKPVHYRDKSPVFSSGKVHKNRRKGLETSVVIDLNIISKMKDVILGKTGYEESGLQYAVKEFNKSPLYLSPGFSFNEADRDYYEELYTSFELFLKKYCPTYIDAPNSLALPPPKESKRTFKELSTGERYMHSISYLSMLLIQIIMRNFRGYSPIQKFEKYVEYMVSKVDFLTVIEAEIARYCFFESNECKDILFKKFCDKIHDNFLKKGNKKYILKNALNSASDIIYYRVVAIQSNEELDGVIQDTWLLTADEGLKYIAQSIYFIPSFDGCDHKAVRLVRNNFQRQSSYWRSCDELFESYNFQRNFINFARFTSQECESKFKLITNCILSLENELVED
ATGGCATTCTCCCCATTGTTTATTAAACCTGTTCATTATCGCGATAAAAGTCCAGTTTTTTCCTCTGGAAAAGTACATAAAAACAGAAGAAAAGGATTGGAGACTAGTGTTGTCATCGATCTAAATATAATATCGAAAATGAAAGATGTTATTTTGGGAAAGACTGGCTATGAGGAATCAGGTTTGCAATATGCTGTTAAGGAATTTAATAAGTCACCATTATATCTTTCTCCCGGATTTTCATTTAACGAAGCCGATCGTGACTATTATGAAGAGCTATATACTTCATTTGAGCTATTTTTAAAAAAATATTGCCCTACTTATATTGATGCTCCAAACTCTTTAGCATTACCACCACCTAAAGAATCTAAACGTACTTTTAAAGAGTTATCAACGGGTGAAAGATATATGCATTCAATATCATATCTTTCTATGCTGTTAATTCAAATAATCATGAGGAATTTTAGAGGTTACTCACCGATACAAAAATTTGAAAAATATGTTGAATATATGGTTTCAAAAGTTGATTTTTTAACTGTAATTGAAGCAGAAATAGCCAGATATTGTTTTTTCGAATCAAATGAATGCAAGGATATACTTTTCAAGAAATTTTGCGATAAAATACACGATAATTTCCTTAAAAAAGGAAACAAAAAATACATATTAAAAAACGCACTAAATTCAGCATCGGATATTATATATTATAGAGTGGTTGCGATTCAATCAAATGAGGAGCTGGATGGAGTAATTCAAGATACTTGGTTACTAACAGCTGATGAAGGTTTAAAATACATAGCACAGTCAATTTATTTTATCCCTTCCTTTGATGGATGTGATCACAAAGCGGTACGATTAGTAAGAAATAATTTTCAGCGACAATCTTCATACTGGCGCAGTTGTGATGAGTTATTTGAATCCTATAATTTTCAACGCAATTTTATTAACTTCGCAAGATTTACTTCTCAAGAGTGCGAGTCAAAATTCAAGCTTATAACTAATTGTATATTATCTTTAGAGAATGAATTGGTTGAAGAT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,560 total letters Query= IDP96899.103 Length=1440 Score E Sequences producing significant alignments: (Bits) Value 2_11F_F5_IDP96899.103_T7_Term.seq 1891 0.0 0_wt 1867 0.0 1_05F_F5_IDP96899.103_v73F.seq 1793 0.0 R N N F V * L * E G D I H M H H H H H H S S G V D L W S H P Query_1 67 AGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCG 156 2 1146 .....A 1141 2 1167 .............. 1154 2 1167 ..C........... 1154 2 243 ............ 232 0 859 ............ 870 1 42 ...........A 53 1 25 ....C........... 40 1 27 .............. 40 1 953 ............ 964 Q F E K G T E N L Y F Q S N A M A F S P L F I K P V H Y R Query_1 157 CAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGGCATTCTCCCCATTGTTTATTAAACC-TGTTCATTATCGCGA 245 2 1140 ........GNN..------.....N.....NNN-..................NTC...................C.NN............ 1058 2 1096 ............. 1108 2 1055 ......... 1063 0 1 .............................-............... 44 0 47 ......... 39 1 54 ........G....------.......................................................-............... 136 1 100 .............. 87 1 139 ......... 131 D K S P V F S S G K V H K N R R K G L E T S V V I D L N I I Query_1 246 TAAAAGTCCAGTTTTTTCCTCTGGAAAAGTACATAAAAACAGAAGAAAA-GGATTGGAGACTAGTGTTGTCATCGATCTAAATATAATAT 334 2 1057 ......................................-..........A........................................ 969 2 1064 ... 1066 0 45 .................................................-........................................ 133 0 38 ... 36 1 137 .................................................-........................................ 225 1 130 ... 128 S K M K D V I L G K T G Y E E S G L Q Y A V K E F N K S P L Query_1 335 CGAAAATGAAAGATGTTATTTTGGGAAAGACTGGCTATGAGGAATCAGGTTTGCAATATGCTGTTAAGGAATTTAATAAGTCACCATTAT 424 2 968 .......................................................................................... 879 2 670 ........... 660 0 134 .......................................................................................... 223 0 432 ........... 442 1 226 .......................................................................................... 315 1 524 ........... 534 Y L S P G F S F N E A D R D Y Y E E L Y T S F E L F L K K Y Query_1 425 ATCTTTCTCCCGGATTTTCATTTAACGAAGCCGATCGTGACTATTATGAAGAGCTATATACTTCATTTGAGCTATTTTTaaaaaaaTATT 514 2 878 .......................................................................................... 789 0 224 .......................................................................................... 313 1 316 .......................................................................................... 405 C P T Y I D A P N S L A L P P P K E S K R T F K E L S T G E Query_1 515 GCCCTACTTATATTGATGCTCCAAACTCTTTAGCATTACCACCACCTAAAGAATCTAAACGTACTTTTAAAGAGTTATCAACGGGTGAAA 604 2 788 .......................................................................................... 699 0 314 .......................................................................................... 403 1 406 .......................................................................................... 495 R Y M H S I S Y L S M L L I Q I I M R N F R G Y S P I Q K F Query_1 605 GATATATGCATTCAATATCATATCTTTCTATGCTGTTAATTCAAATAATCATGAGGAATTTTAGAGGTTACTCACCGATACAAAAATTTG 694 2 698 .......................................................................................... 609 2 912 ........... 902 0 404 .......................................................................................... 493 0 190 ........... 200 1 496 .......................................................................................... 585 1 282 ........... 292 E K Y V E Y M V S K V D F L T V I E A E I A R Y C F F E S N Query_1 695 AAAAATATGTTGAATATATGGTTTCAAAAGTTGATTTTTTAACTGTAATTGAAGCAGAAATAGCCAGATATTGTTTTTTCGAATCAAATG 784 2 608 .......................................................................................... 519 0 494 .......................................................................................... 583 1 586 .......................................................................................... 675 E C K D I L F K K F C D K I H D N F L K K G N K K Y I L K N Query_1 785 AATGCAAGGATATACTTTTCAAGAAATTTTGCGATAAAATACACGATAATTTCCTTaaaaaaggaaacaaaaaatacatatt-aaaaaaC 873 2 518 ..................................................................................-....... 430 0 584 ..................................................................................-....... 672 1 676 ..................................................................................A....... 765 A L N S A S D I I Y Y R V V A I Q S N E E L D G V I Q D T W Query_1 874 GCACTAAATTCAGCATCGGATATTATATATTATAGAGTGGTTGCGATTCAATCAAATGAGGAGCTGGATGGAGTAATTCAAGATACTTGG 963 2 429 .......................................................................................... 340 0 673 .......................................................................................... 762 1 766 .......................................................................................... 855 L L T A D E G L K Y I A Q S I Y F I P S F D G C D H K A V Query_1 964 TTACTAACAGCTGATGAA-GGTTTAAAATACATAGCACAGTCAATTTATTTTATCCCTTCCTTTGATGGATGTGATCACAAAGCGGTACG 1052 2 339 ..................-....................................................................... 251 0 763 ..................-....................................................................... 851 1 856 ..................N....................................................................... 945 R L V R N N F Q R Q S S Y W R S C D E L F E S Y N F Q R N Query_1 1053 ATTAGTAAGAAATAATTTTCAGCGACAATC-TTCA-TACTGGCGCAGTTGTGATGAGTTATTTG-AATCCT-ATAATTTTCAACGCAATT 1138 2 250 ..............................-....-............................-......-.................. 165 0 852 ..............................-....-............................-......-.................. 937 1 946 ..N........................T..T....T....................N.......A......A...N...N.......... 1035 F I N F A R F T S Q E C E S K F K L I T N C I L S L E N Query_1 1139 TTATTAACTTCGCAAGATTTACTT-CTCAA-GAG-TGCGAGTCAAAATTCAAGCTT-ATAACTAATTGTATA-TTATCTTTAG-AGAATG 1222 2 164 ........................-.....-...-.....................-...............-..........-...... 81 0 938 ........................-.....-...-.....................-...............-..........-...... 1021 1 1036 ........................N.....G...N.....NNNN...NN.......A.........NNAT..T..........A.N.... 1125 E L V E D * H W K W I T D P N S S S V D K L A A A L E H H H Query_1 1223 AATTGGTTGAAGATTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccacc 1312 2 80 ..................................--..... 42 0 1022 .............. 1035 1 1126 ......NN...N.....N.N.NNN.N..AN.... 1159 H H H * D P A A N K A R K E A E L A A A T A E Q * L Query_1 1313 accaccacTGAGATCCGGCTGCTAACAAAG-CCCGAAAGGAAGCTG-AGTTGGCTGCTGCCACCGCTGAGCAATAACTAG 1390 2 46 ..................C...N....... 17 1 1175 ...G.-...............N.NNN.........NNA...N..A..N..C.... 1228 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5001088 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 3,560 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96902.103 (IDP96902.51-349.P(349)..pMCSG68)
QKVEVSRKVETDAPPQIIYRIDKNRYLTLENYISCDKGGQVYYNDSQRGIKTHLGWDIEFYDFSYRRGNNLVAYKGSVINGADNGYLALPGASTRQYCGSGRSERGCPLFFFFSADHGKTFIYKIIAEEYSTPERFSKLKVVVANDGVYLKDESQTESVFSHPIGLRDLHSVNKLIFSDGKLISVYDDWQKKVHELVRKELIRKKMPYANDYGPTFHIFDYIRKLTPPKTHEERNLMANELSDIRIRIEDEVYSHRIDFPKLSTKSIDAKYLCNKSIEAKIITYTNESNGKKEVVKNEQ
CAAAAGGTTGAAGTGTCGCGGAAAGTTGAAACGGATGCTCCGCCACAAATTATTTATCGCATAGATAAAAATCGATACTTGACGCTGGAAAACTACATATCCTGTGATAAGGGTGGACAGGTATATTACAATGACTCTCAGCGGGGAATAAAAACACACCTGGGATGGGATATCGAATTTTATGATTTTTCCTATCGCCGGGGAAATAATTTAGTAGCTTATAAAGGCTCTGTTATTAATGGGGCAGATAATGGTTATCTGGCACTCCCCGGCGCATCTACCAGACAATATTGTGGTAGTGGAAGGTCAGAGCGAGGTTGTCCTTTGTTTTTCTTCTTTTCAGCAGATCATGGAAAAACCTTTATCTATAAAATTATAGCTGAGGAGTATAGTACCCCTGAACGGTTCAGTAAGCTTAAAGTGGTAGTGGCTAATGATGGGGTTTATTTAAAAGATGAGTCTCAAACAGAGAGTGTATTTTCTCACCCTATAGGTCTTCGCGATCTTCATTCAGTGAATAAATTAATATTTTCAGACGGAAAATTAATCAGTGTTTATGATGATTGGCAGAAAAAAGTTCATGAGTTAGTGAGAAAAGAACTGATAAGAAAAAAAATGCCTTATGCAAATGATTATGGACCCACATTTCATATATTCGACTATATCAGAAAATTAACACCTCCTAAAACTCATGAGGAGAGAAATTTGATGGCAAATGAATTGTCAGATATTCGAATCAGGATTGAGGATGAGGTTTATAGTCACAGGATTGATTTTCCGAAACTTTCAACGAAAAGTATCGATGCTAAATATCTATGTAATAAAAGCATTGAAGCTAAAATCATCACATATACCAATGAGTCAAATGGGAAAAAGGAGGTGGTAAAAAATGAACAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 907 total letters Query= IDP96902.103 Length=1302 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1618 0.0 Q K V E V S R K V E T D A P P Q I I Y R I D K N R Y L T L E Query_1 202 CAAAAGGTTGAAGTGTCGCGGAAAGTTGAAACGGATGCTCCGCCACAAATTATTTATCGCATAGATAAAAATCGATACTTGACGCTGGAA 291 0 1 .......................................................................................... 90 N Y I S C D K G G Q V Y Y N D S Q R G I K T H L G W D I E F Query_1 292 AACTACATATCCTGTGATAAGGGTGGACAGGTATATTACAATGACTCTCAGCGGGGAATAAAAACACACCTGGGATGGGATATCGAATTT 381 0 91 .......................................................................................... 180 Y D F S Y R R G N N L V A Y K G S V I N G A D N G Y L A L P Query_1 382 TATGATTTTTCCTATCGCCGGGGAAATAATTTAGTAGCTTATAAAGGCTCTGTTATTAATGGGGCAGATAATGGTTATCTGGCACTCCCC 471 0 181 .......................................................................................... 270 G A S T R Q Y C G S G R S E R G C P L F F F F S A D H G K T Query_1 472 GGCGCATCTACCAGACAATATTGTGGTAGTGGAAGGTCAGAGCGAGGTTGTCCTTTGTTTTTCTTCTTTTCAGCAGATCATGGAAAAACC 561 0 271 .......................................................................................... 360 F I Y K I I A E E Y S T P E R F S K L K V V V A N D G V Y L Query_1 562 TTTATCTATAAAATTATAGCTGAGGAGTATAGTACCCCTGAACGGTTCAGTAAGCTTAAAGTGGTAGTGGCTAATGATGGGGTTTATTTA 651 0 361 .......................................................................................... 450 K D E S Q T E S V F S H P I G L R D L H S V N K L I F S D G Query_1 652 AAAGATGAGTCTCAAACAGAGAGTGTATTTTCTCACCCTATAGGTCTTCGCGATCTTCATTCAGTGAATAAATTAATATTTTCAGACGGA 741 0 451 .......................................................................................... 540 K L I S V Y D D W Q K K V H E L V R K E L I R K K M P Y A N Query_1 742 AAATTAATCAGTGTTTATGATGATTGGCAGAAAAAAGTTCATGAGTTAGTGAGAAAAGAACTGATAAGaaaaaaaaTGCCTTATGCAAAT 831 0 541 .......................................................................................... 630 D Y G P T F H I F D Y I R K L T P P K T H E E R N L M A N E Query_1 832 GATTATGGACCCACATTTCATATATTCGACTATATCAGAAAATTAACACCTCCTAAAACTCATGAGGAGAGAAATTTGATGGCAAATGAA 921 0 631 .......................................................................................... 720 L S D I R I R I E D E V Y S H R I D F P K L S T K S I D A K Query_1 922 TTGTCAGATATTCGAATCAGGATTGAGGATGAGGTTTATAGTCACAGGATTGATTTTCCGAAACTTTCAACGAAAAGTATCGATGCTAAA 1011 0 721 .......................................................................................... 810 Y L C N K S I E A K I I T Y T N E S N G K K E V V K N E Q Query_1 1012 TATCTATGTAATAAAAGCATTGAAGCTAAAATCATCACATATACCAATGAGTCAAATGGGAAAAAGGAGGTGGTAAAAAATGAACAG 1098 0 811 ....................................................................................... 897 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1114120 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:50 PM Number of letters in database: 907 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96903.103 (IDP96903.1-141.F..pMCSG73)
MRQRTIVCPLIENEGHYLLCKMAADRGVFPGQWALSGGGVEPGERIEEALRREIREELGEKLILTHIAPWCFRDDTRVKTYPDGHQETIYMIYLIFNCVSANRDVTINEEFDDYAWVKAEDLKNYDLNAATRVTLSLKGLL
ATGCGCCAAAGGACGATTGTTTGCCCGCTGATCGAGAATGAAGGCCATTATCTGCTTTGCAAAATGGCCGCCGACCGCGGGGTTTTTCCCGGGCAATGGGCCTTATCCGGCGGCGGCGTGGAGCCAGGGGAACGTATTGAAGAGGCGCTGCGGCGGGAAATACGCGAAGAGCTCGGCGAAAAACTCATCCTTACCCATATCGCGCCATGGTGCTTTCGCGACGATACCCGCGTCAAAACCTACCCCGACGGGCATCAGGAAACGATCTACATGATCTATCTCATCTTCAACTGCGTGAGCGCCAATCGCGATGTGACGATCAACGAGGAGTTTGACGATTACGCCTGGGTCAAAGCCGAAGATCTCAAAAACTACGATCTTAATGCCGCCACCCGCGTTACTCTCAGCCTGAAAGGGCTACTT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,917 total letters Query= IDP96903.103 Length=828 Score E Sequences producing significant alignments: (Bits) Value 2_11G_G5_IDP96903.103_T7_Term.seq 868 0.0 1_05G_G5_IDP96903.103_v73F.seq 868 0.0 0_wt 764 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 2 540 ...........A........G....------....................... 493 2 558 .....C........... 542 1 43 ...........A........G....------....................... 90 1 27 ...C........... 41 1 28 .............. 41 A M R Q R T I V C P L I E N E G H Y L L C K M A A D R G V F Query_1 199 GCCATGCGCCAAAGGACGATTGTTTGCCCGCTGATCGAGAATGAAGGCCATTATCTGCTTTGCAAAATGGCCGCCGACCGCGGGGTTTTT 288 2 492 .......................................................................................... 403 1 91 .......................................................................................... 180 0 1 ....................................................................................... 87 P G Q W A L S G G G V E P G E R I E E A L R R E I R E E L G Query_1 289 CCCGGGCAATGGGCCTTATCCGGCGGCGGCGTGGAGCCAGGGGAACGTATTGAAGAGGCGCTGCGGCGGGAAATACGCGAAGAGCTCGGC 378 2 402 .......................................................................................... 313 1 181 .......................................................................................... 270 0 88 .......................................................................................... 177 E K L I L T H I A P W C F R D D T R V K T Y P D G H Q E T I Query_1 379 GAAAAACTCATCCTTACCCATATCGCGCCATGGTGCTTTCGCGACGATACCCGCGTCAAAACCTACCCCGACGGGCATCAGGAAACGATC 468 2 312 .......................................................................................... 223 1 271 .......................................................................................... 360 0 178 .......................................................................................... 267 Y M I Y L I F N C V S A N R D V T I N E E F D D Y A W V K A Query_1 469 TACATGATCTATCTCATCTTCAACTGCGTGAGCGCCAATCGCGATGTGACGATCAACGAGGAGTTTGACGATTACGCCTGGGTCAAAGCC 558 2 222 .......................................................................................... 133 1 361 .......................................................................................... 450 0 268 .......................................................................................... 357 E D L K N Y D L N A A T R V T L S L K G L L * H W K W I T D Query_1 559 GAAGATCTCAAAAACTACGATCTTAATGCCGCCACCCGCGTTACTCTCAGCCTGAAAGGGCTACTTTAACATTGGAAGTGGATAACGGAT 648 2 132 ......................................................................................--.. 45 1 451 ......................................................................................--.. 538 0 358 .................................................................. 423 P N S S S V D K L A A A L E H H H H H H * D P A A N K A R Query_1 649 CCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCG--AA 736 2 44 ... 42 2 46 ......................NA.. 21 1 539 ... 541 1 537 ......................--.. 560 K E A E L A A A T A E Q * L A * P L G A S K R V L R G F L L Query_1 737 AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGA 826 2 20 ...... 15 1 561 .......................................................................................... 650 K Query_1 827 AA 828 1 651 .. 652 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2334936 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:51 PM Number of letters in database: 2,917 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96907.102 (IDP96907.23-138.P(138)..pMCSG68)
KWHGCQEQNLIFFAYNIKHTKAVELCQTEEGYRYTFGPIGKPEIILEKDSNDVTRGGGMAGGFDIKNGDIIYGVMEDKFGNSALIVQRSDYSKVLAKIELDSVSKSYVNKTFEYLH
AAATGGCATGGATGTCAAGAGCAAAACCTTATTTTCTTTGCTTATAACATAAAACATACTAAAGCGGTAGAACTCTGCCAAACGGAGGAAGGTTATAGATACACTTTTGGACCTATTGGAAAACCAGAAATTATTCTAGAAAAAGATAGCAACGATGTTACTCGCGGAGGAGGTATGGCTGGAGGATTTGATATTAAAAATGGTGATATTATTTACGGAGTAATGGAAGATAAATTTGGTAATTCTGCTCTCATCGTACAGAGGTCTGATTATAGTAAAGTCTTGGCGAAAATTGAACTTGATAGTGTAAGCAAGAGCTATGTTAATAAAACCTTTGAATATCTTCAT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 358 total letters Query= IDP96907.102 Length=753 Score E Sequences producing significant alignments: (Bits) Value 0_wt 628 0.0 K W H G C Q E Q N L I F F A Y N I K H T K A V E L C Q T E E Query_1 202 AAATGGCATGGATGTCAAGAGCAAAACCTTATTTTCTTTGCTTATAACATAAAACATACTAAAGCGGTAGAACTCTGCCAAACGGAGGAA 291 0 1 .......................................................................................... 90 G Y R Y T F G P I G K P E I I L E K D S N D V T R G G G M A Query_1 292 GGTTATAGATACACTTTTGGACCTATTGGAAAACCAGAAATTATTCTAGAAAAAGATAGCAACGATGTTACTCGCGGAGGAGGTATGGCT 381 0 91 .......................................................................................... 180 G G F D I K N G D I I Y G V M E D K F G N S A L I V Q R S D Query_1 382 GGAGGATTTGATATTAAAAATGGTGATATTATTTACGGAGTAATGGAAGATAAATTTGGTAATTCTGCTCTCATCGTACAGAGGTCTGAT 471 0 181 .......................................................................................... 270 Y S K V L A K I E L D S V S K S Y V N K T F E Y L H Query_1 472 TATAGTAAAGTCTTGGCGAAAATTGAACTTGATAGTGTAAGCAAGAGCTATGTTAATAAAACCTTTGAATATCTTCAT 549 0 271 .............................................................................. 348 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 238602 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:51 PM Number of letters in database: 358 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96908.103 (IDP96908.1-85.F..pMCSG73)
MSLLLQPVEINLPDTVGAAMRQTGFSLCTAAERSSLYMLPLPAPHRHSRRIPLRDALQKEKLARQDAPALGLYVVTLRYQATSTG
ATGAGCCTGCTGCTACAGCCCGTCGAAATTAACCTTCCCGATACGGTTGGCGCCGCAATGCGCCAGACGGGCTTTAGTCTGTGTACCGCCGCCGAACGTTCATCGCTCTATATGCTTCCGCTGCCCGCCCCCCACCGACACAGCAGGCGAATACCGCTGCGCGACGCCCTACAGAAGGAGAAACTGGCGCGGCAAGACGCGCCAGCTCTGGGTTTATACGTGGTTACCTTGCGTTATCAGGCAACCAGTACCGGT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 265 total letters Query= IDP96908.103 Length=660 Score E Sequences producing significant alignments: (Bits) Value 0_wt 461 2e-134 M S L L L Q P V E I N L P D T V G A A M R Q T G F S L C T A Query_1 202 ATGAGCCTGCTGCTACAGCCCGTCGAAATTAACCTTCCCGATACGGTTGGCGCCGCAATGCGCCAGACGGGCTTTAGTCTGTGTACCGCC 291 0 1 .......................................................................................... 90 A E R S S L Y M L P L P A P H R H S R R I P L R D A L Q K E Query_1 292 GCCGAACGTTCATCGCTCTATATGCTTCCGCTGCCCGCCCCCCACCGACACAGCAGGCGAATACCGCTGCGCGACGCCCTACAGAAGGAG 381 0 91 .......................................................................................... 180 K L A R Q D A P A L G L Y V V T L R Y Q A T S T G Query_1 382 AAACTGGCGCGGCAAGACGCGCCAGCTCTGGGTTTATACGTGGTTACCTTGCGTTATCAGGCAACCAGTACCGGT 456 0 181 ........................................................................... 255 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 148392 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:51 PM Number of letters in database: 265 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96910.102 (IDP96910.1-253.F..pMCSG68)
MDDLLDAITALSSLISSEKIRAIAENISHIDDNKSMATLYNVVNTPIAVTIVKQLTMAWQKTSVSSSELAAMLLTASHVYSKATSEQSTELVWTGPTTPFVSARRTEQALLQVISTAERTLFITSFVAYDVSTIVKALNAANERGVMISILLELSQEKGGSLNFDTIEKMRIQVPSAKLYVWNNKDNEYYGGCVHAKVAVADARECFITSANLTGYAMEKNIEAGVLISGGSIPRQLNEHLFSLIKTKIICLV
ATGGATGATCTTCTGGACGCTATTACAGCGCTGAGCTCTCTAATATCATCTGAAAAAATAAGAGCTATCGCTGAAAATATTAGCCATATTGATGATAATAAATCTATGGCCACATTATACAATGTGGTCAATACTCCAATAGCTGTAACAATAGTCAAACAACTCACTATGGCCTGGCAAAAAACGTCTGTCAGTTCTAGTGAGTTGGCTGCAATGCTCCTTACTGCCAGCCATGTCTATTCAAAAGCCACTTCAGAGCAATCTACCGAGTTAGTGTGGACGGGCCCCACAACACCATTTGTTTCAGCACGTCGTACTGAACAGGCTTTGCTTCAAGTTATTAGTACCGCTGAGCGCACTCTGTTTATAACCAGCTTCGTCGCTTATGATGTTTCAACGATAGTGAAAGCATTAAATGCTGCAAATGAACGCGGAGTTATGATCTCAATTTTACTTGAATTATCACAAGAGAAAGGTGGTAGTTTAAATTTTGATACCATTGAGAAGATGAGAATACAGGTCCCTTCAGCCAAACTTTATGTATGGAATAATAAAGATAATGAATATTATGGCGGATGTGTTCATGCAAAAGTTGCTGTTGCTGATGCTCGGGAGTGCTTTATCACTAGTGCAAACCTTACTGGGTATGCTATGGAAAAAAATATTGAAGCTGGGGTGCTTATTTCTGGCGGAAGTATTCCAAGGCAACTTAATGAGCATCTTTTTTCCCTTATTAAAACTAAAATTATCTGCTTGGTT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,231 total letters Query= IDP96910.102 Length=1164 Score E Sequences producing significant alignments: (Bits) Value 1_02E_E2_IDP96910.102_T7S.seq 1882 0.0 2_08E_E2_IDP96910.102_T7term.seq 1864 0.0 0_wt 1370 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L Query_1 1 TCTCGAT-CCCGCGAAA-TTAATACGACTCACTATAGGGGAA-TTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTT 87 1 8 ...........-................ 34 2 1098 .......T.........A.....N.......NAT........A...........AN..........N.......AT.A............ 1009 2 1028 ...GN......-....NTT.C.C.C......T... 1061 * E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E Query_1 88 TAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAG 177 1 35 .......................................................................................... 124 2 1008 .......................................................................................... 919 N L Y F Q S N A M D D L L D A I T A L S S L I S S E K I R A Query_1 178 AACCTGTACTTCCAATCCAATGCCATGGATGATCTTCTGGACGCTATTACAGCGCTGAGCTCTCTAATATCATCTGAAAAAATAAGAGCT 267 1 125 .......................................................................................... 214 2 918 .......................................................................................... 829 0 1 .................................................................. 66 I A E N I S H I D D N K S M A T L Y N V V N T P I A V T I V Query_1 268 ATCGCTGAAAATATTAGCCATATTGATGATAATAAATCTATGGCCACATTATACAATGTGGTCAATACTCCAATAGCTGTAACAATAGTC 357 1 215 .......................................................................................... 304 2 828 .......................................................................................... 739 0 67 .......................................................................................... 156 K Q L T M A W Q K T S V S S S E L A A M L L T A S H V Y S K Query_1 358 AAACAACTCACTATGGCCTGGCAAAAAACGTCTGTCAGTTCTAGTGAGTTGGCTGCAATGCTCCTTACTGCCAGCCATGTCTATTCAAAA 447 1 305 .......................................................................................... 394 1 1024 ............ 1035 2 738 .......................................................................................... 649 0 157 .......................................................................................... 246 A T S E Q S T E L V W T G P T T P F V S A R R T E Q A L L Q Query_1 448 GCCACTTCAGAGCAATCTACCGAGTTAGTGTGGACGGGCCCCACAACACCATTTGTTTCAGCACGTCGTACTGAACAGGCTTTGCTTCAA 537 1 395 .......................................................................................... 484 2 648 .......................................................................................... 559 0 247 .......................................................................................... 336 V I S T A E R T L F I T S F V A Y D V S T I V K A L N A A N Query_1 538 GTTATTAGTACCGCTGAGCGCACTCTGTTTATAACCAGCTTCGTCGCTTATGATGTTTCAACGATAGTGAAAGCATTAAATGCTGCAAAT 627 1 485 .......................................................................................... 574 2 558 .......................................................................................... 469 0 337 .......................................................................................... 426 E R G V M I S I L L E L S Q E K G G S L N F D T I E K M R I Query_1 628 GAACGCGGAGTTATGATCTCAATTTTACTTGAATTATCACAAGAGAAAGGTGGTAGTTTAAATTTTGATACCATTGAGAAGATGAGAATA 717 1 575 .......................................................................................... 664 2 468 .......................................................................................... 379 0 427 .......................................................................................... 516 Q V P S A K L Y V W N N K D N E Y Y G G C V H A K V A V A D Query_1 718 CAGGTCCCTTCAGCCAAACTTTATGTATGGAATAATAAAGATAATGAATATTATGGCGGATGTGTTCATGCAAAAGTTGCTGTTGCTGAT 807 1 665 .......................................................................................... 754 2 378 .......................................................................................... 289 0 517 .......................................................................................... 606 A R E C F I T S A N L T G Y A M E K N I E A G V L I S G G S Query_1 808 GCTCGGGAGTGCTTTATCACTAGTGCAAACCTTACTGGGTATGCTATGGaaaaaaaTATTGAAGCTGGGGTGCTTATTTCTGGCGGAAGT 897 1 755 .......................................................................................... 844 2 288 .......................................................................................... 199 0 607 .......................................................................................... 696 I P R Q L N E H L F S L I K T K I I C L V * H W K W I T D P Query_1 898 ATTCCAAGGCAACTTAATGAGCATCTTTTTTCCCTTATTAAAACTAAAATTATCTGCTTGGTTTAACATTGGAAGTGGATAACGGATCCG 987 1 845 .............................................-..................-......................... 932 2 198 .......................................................................................... 109 0 697 ............................................................... 759 N S S S V D K L A A A L E H H H H H H * D P A A N K A R K Query_1 988 AATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTG-AGATCCGGCTGCTAACAAAGCCC--GAAAG 1074 1 933 ...........................................................-.......................--.-... 1018 2 108 ...........................................................A.......................CGA.... 19 E A E L A A A T A E Q * L A * P L G A S K R V L R G F L L Query_1 1075 GAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGC-ATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAA 1163 1 1019 ................................N.-......N...--.....T...N......--N.NN.N..GA...G........... 1103 1 349 ............ 360 2 18 ... 16 2 694 ............ 683 0 201 ............ 212 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3654084 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:51 PM Number of letters in database: 3,231 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96911.103 (IDP96911.1-467.F..pMCSG73)
MNTRQLLSVGIDIGTTTTQVIFSRLELVNRAAVSQVPRYEFIKRDISWQSPVFFTPVDKKGELKEAELEALILAQYRAAGIAPQAVDSGAIIITGESAKTRNARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSRQRMCRVLNIDIGGGTSNYALFDAGNVSATACLNVGGRLLETDAQGRVVHAHPPGQRIVDALFGAGTNALALTAGQLAQVARRMAELIVEVIDGTLSPLAQGLMQTEVLPAGIQPEVITLSGGVGECYRHQPADPFCFSDIGPLLATALHEHPRLREMNVQFPTQTVRATVIGAGAHTLSLSGSTIWLEGVPLPLRNLPVAIPQDAADLPNAWLQALTQLDLAPEADAYVLALPASLPVRYATLLTVIDALLAFVARFPNPRPLLLVAEQDFGKALGMLLRPQLPHLPLAVIDEVSIRAGDYIDIGTPLFGGSVVPVTVKSLAFPS
GTGAACACGCGCCAGCTGTTGAGCGTCGGTATTGATATCGGCACCACCACCACGCAGGTGATCTTCTCGCGCCTCGAGCTGGTGAACCGCGCGGCGGTTTCCCAGGTGCCGCGTTATGAATTCATCAAACGCGACATTAGCTGGCAAAGCCCGGTCTTCTTTACGCCGGTTGATAAAAAGGGAGAATTGAAAGAGGCCGAGCTTGAGGCGCTGATTCTGGCTCAGTACCGGGCCGCAGGGATCGCTCCACAAGCGGTGGATTCCGGGGCGATCATCATCACCGGCGAAAGCGCGAAAACCCGCAATGCCCGCCCGGCGGTGATGGCGCTCTCGCAGTCGCTGGGCGATTTTGTCGTCGCCAGCGCCGGGCCGCATCTGGAGTCGGTCATTGCCGGACACGGCGCCGGGGCGCAGACCCTGTCCCGGCAGCGGATGTGCCGGGTGCTCAATATCGATATTGGCGGCGGTACCTCGAACTATGCCCTGTTTGACGCCGGAAACGTCAGCGCCACCGCCTGCCTCAACGTCGGCGGCCGCCTGCTGGAAACCGACGCTCAGGGGCGCGTGGTCCACGCGCACCCGCCGGGGCAGCGGATTGTCGATGCGCTGTTTGGCGCCGGCACTAACGCCCTGGCGCTGACCGCCGGGCAGCTGGCGCAGGTGGCGCGCCGGATGGCGGAGCTGATCGTCGAGGTGATCGACGGCACCCTCTCACCGCTGGCGCAGGGGCTGATGCAAACCGAAGTACTGCCTGCGGGTATTCAGCCGGAGGTGATTACCCTTTCCGGCGGAGTGGGGGAGTGCTACCGCCATCAGCCCGCCGATCCTTTCTGTTTTTCCGATATCGGGCCGCTGCTGGCCACGGCCCTGCATGAGCATCCGCGCCTGCGTGAGATGAACGTGCAGTTTCCGACGCAAACCGTGCGCGCCACGGTGATTGGCGCCGGGGCGCATACCCTGTCCCTGTCCGGGAGCACCATCTGGCTGGAGGGGGTTCCATTGCCGCTGCGCAATCTGCCGGTGGCTATCCCGCAGGATGCCGCCGATCTGCCGAACGCCTGGCTCCAGGCGCTGACGCAGCTCGATCTGGCCCCCGAGGCGGACGCTTACGTGCTGGCGCTTCCCGCTTCACTTCCGGTGCGCTACGCCACCTTACTGACTGTAATTGACGCCCTGCTGGCGTTTGTCGCCCGCTTTCCCAATCCCCGTCCGCTGCTGCTGGTGGCCGAGCAGGATTTTGGCAAAGCGCTGGGCATGCTGCTGCGGCCACAGCTTCCACACCTTCCGCTGGCGGTCATCGATGAGGTGAGCATCCGGGCGGGAGACTATATCGACATTGGTACGCCTCTTTTTGGCGGATCGGTTGTGCCGGTGACGGTGAAATCACTCGCATTTCCTTCC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,880 total letters Query= IDP96911.103 Length=1806 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2527 0.0 2_12A_A6_IDP96911.103_T7_Term.seq 1911 0.0 1_06A_A6_IDP96911.103_v73F.seq 1865 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 1 43 ...........A........G....------....................... 90 1 27 ...C........... 41 1 28 .............. 41 A V N T R Q L L S V G I D I G T T T T Q V I F S R L E L V N Query_1 199 GCCGTGAACACGCGCCAGCTGTTGAGCGTCGGTATTGATATCGGCACCACCACCACGCAGGTGATCTTCTCGCGCCTCGAGCTGGTGAAC 288 0 1 ....................................................................................... 87 0 557 ........... 547 2 908 ........... 918 1 91 ...A...................................................................................... 180 1 650 ........... 640 R A A V S Q V P R Y E F I K R D I S W Q S P V F F T P V D K Query_1 289 CGCGCGGCGGTTTCCCAGGTGCCGCGTTATGAATTCATCAAACGCGACATTAGCTGGCAAAGCCCGGTCTTCTTTACGCCGGTTGATAAA 378 0 88 .......................................................................................... 177 1 181 .......................................................................................... 270 K G E L K E A E L E A L I L A Q Y R A A G I A P Q A V D S G Query_1 379 AAGGGAGAATTGAAAGAGGCCGAGCTTGAGGCGCTGATTCTGGCTCAGTACCGGGCCGCAGGGATCGCTCCACAAGCGGTGGATTCCGGG 468 0 178 .......................................................................................... 267 2 1206 .. 1205 1 271 .......................................................................................... 360 A I I I T G E S A K T R N A R P A V M A L S Q S L G D F Query_1 469 GCGAT-CAT-CATCACCGGCGAAAGCGCGAAAA--CCCGCAATGCCCGCCCGGCGGTGA-TGGCGCTCTCGCAGTCGCTGGGCG-ATTTT 552 0 268 .....-...-.......................--........................-........................-..... 351 2 1204 .....T...T...NN.-.N....N.....G...NN.....C.NTN......N.N.....A......NNNN-....NN.......G....N 1117 1 361 .....-...-.......................--........................-........................-..... 444 1 32 ..-...-......T.. 45 V V A S A G P H L E S V I A G H G A G A Q T L S R Q R M Query_1 553 GTCGTCGCC-AGCGCC-GGGCCGC-ATCTGGAGTCGGTCATTGCCGGACACGGCGCCGGGGC-GCAGACCCTGTCCCGGCAGCGGATGTG 638 0 352 .........-......-.......-.....................................-........................... 437 0 940 ...........-...T.......... 964 2 1116 .........C......C.NN....C........................N..NNN......NN........................... 1027 2 525 ...........-...T.......... 501 1 445 .........-......-.......-.....................................-........................... 530 C R V L N I D I G G G T S N Y A L F D A G N V S A T A C L N Query_1 639 CCGGGTGCTCAATATCGATATTGGCGGCGGTACCTCGAACTATGCCCTGTTTGACGCCGGAAACGTCAGCGCCACCGCCTGCCTCAACGT 728 0 438 .......................................................................................... 527 0 460 .............. 447 2 1026 ...................................................-...................................... 938 2 1004 .............. 1017 1 531 .......................................................................................... 620 1 553 .............. 540 V G G R L L E T D A Q G R V V H A H P P G Q R I V D A L F G Query_1 729 CGGCGGCCGCCTGCTGGAAACCGACGCTCAGGGGCGCGTGGTCCACGCGCACCCGCCGGGGCAGCGGATTGTCGATGCGCTGTTTGGCGC 818 0 528 .......................................................................................... 617 0 30 ........... 20 2 937 .......................................................................................... 848 1 621 .......................................................................................... 710 1 123 ........... 113 A G T N A L A L T A G Q L A Q V A R R M A E L I V E V I D G Query_1 819 CGGCACTAACGCCCTGGCGCTGACCGCCGGGCAGCTGGCGCAGGTGGCGCGCCGGATGGCGGAGCTGATCGTCGAGGTGATCGACGGCAC 908 0 618 .......................................................................................... 707 0 717 ........... 727 2 847 .......................................................................................... 758 2 748 ........... 738 1 711 .......................................................................................... 800 1 810 ........... 820 T L S P L A Q G L M Q T E V L P A G I Q P E V I T L S G G V Query_1 909 CCTCTCACCGCTGGCGCAGGGGCTGATGCAAACCGAAGTACTGCCTGCGGGTATTCAGCCGGAGGTGATTACCCTTTCCGGCGGAGTGGG 998 0 708 .......................................................................................... 797 0 651 ........... 661 2 757 .......................................................................................... 668 2 814 ........... 804 1 801 .......................................................................................... 890 1 744 ........... 754 G E C Y R H Q P A D P F C F S D I G P L L A T A L H E H P R Query_1 999 GGAGTGCTACCGCCATCAGCCCGCCGATCCTTTCTGTTTTTCCGATATCGGGCCGCTGCTGGCCACGGCCCTGCATGAGCATCCGCGCCT 1088 0 798 .......................................................................................... 887 2 667 .......................................................................................... 578 1 891 .......................................................................................... 980 L R E M N V Q F P T Q T V R A T V I G A G A H T L S L S G S Query_1 1089 GCGTGAGATGAACGTGCAGTTTCCGACGCAAACCGTGCGCGCCACGGTGATTGGCGCCGGGGCGCATACCCTGTCCCTGTCCGGGAGCAC 1178 0 888 .......................................................................................... 977 0 400 ..............G.......... 424 2 577 .......................................................................................... 488 1 981 ...............................................N..........NN..NN.....N....-.......-......- 1067 1 493 ..............G.......... 517 T I W L E G V P L P L R N L P V A I P Q D A A D L P N A W Query_1 1179 CATCTGGCTGGA-GGGGGTTCCATTGCCGCTGCGCAATCTGCCGGTGGCTATCCCGCAGG-ATGCCGCCGATCTGCCGAACGCCTGGC-- 1264 0 978 ............-...............................................-...........................-- 1063 2 487 ............-...............................................-...........................-- 402 1 1068 ............G......................-........N.-..N..T.......A.....-NN.N...TGN.-.......NNNT 1153 L Q A L T Q L D L A P E A D A Y V L A L P A S L P V R Y A T Query_1 1265 TCCAGGCGCTGACGCAGCTCGATCTGGCCCCCGAGGCGGACGCTTACGTGCTGGCGCTTCCCGCTTCACTTCCGGTGCGCTACGCCACCT 1354 0 1064 .......................................................................................... 1153 2 401 .......................................................................................... 312 1 1154 N.....GNN.......N........ 1178 L L T V I D A L L A F V A R F P N P R P L L L V A E Q D F G Query_1 1355 TACTGACTGTAATTGACGCCCTGCTGGCGTTTGTCGCCCGCTTTCCCAATCCCCGTCCGCTGCTGCTGGTGGCCGAGCAGGATTTTGGCA 1444 0 1154 .......................................................................................... 1243 2 311 .......................................................................................... 222 K A L G M L L R P Q L P H L P L A V I D E V S I R A G D Y I Query_1 1445 AAGCGCTGGGCATGCTGCTGCGGCCACAGCTTCCACACCTTCCGCTGGCGGTCATCGATGAGGTGAGCATCCGGGCGGGAGACTATATCG 1534 0 1244 .......................................................................................... 1333 2 221 .......................................................................................... 132 D I G T P L F G G S V V P V T V K S L A F P S * H W K W I T Query_1 1535 ACATTGGTACGCCTCTTTTTGGCGGATCGGTTGTGCCGGTGACGGTGAAATCACTCGCATTTCCTTCCTAACATTGGAAGTGGATAACGG 1624 0 1334 .................................................................... 1401 2 131 ........................................................................................-- 44 D P N S S S V D K L A A A L E H H H H H H * D P A A N K A R Query_1 1625 ATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAA 1714 2 43 ..... 39 2 43 .....................N.. 20 K E Query_1 1715 AGGAAG 1720 2 19 ..A... 14 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 6850081 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:51 PM Number of letters in database: 3,880 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96914.103 (IDP96914.1-76.F..pMCSG73)
MSSIYSDEYQLVIKLLRSARIDRGITQSQLAKSLGKPQSFVAKVENGERRLDVIEFAAIARLLSLDPAEILNAVMK
ATGTCATCTATTTATTCTGATGAATATCAGTTAGTTATAAAGTTACTGCGCTCTGCAAGAATTGATCGGGGGATTACTCAATCTCAACTGGCTAAATCGCTGGGCAAACCCCAGTCATTCGTTGCAAAGGTGGAGAATGGCGAACGTCGTTTGGATGTGATTGAATTTGCAGCTATTGCTCGCCTGCTTTCGCTTGATCCCGCAGAAATACTGAACGCAGTTATGAAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,746 total letters Query= IDP96914.103 Length=633 Score E Sequences producing significant alignments: (Bits) Value 2_12B_B6_IDP96914.103_T7_Term.seq 509 6e-148 1_06B_B6_IDP96914.103_v73F.seq 506 7e-147 0_wt 412 1e-118 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGT-ACTTCCAATCCAA 197 2 346 ...........A........G....------.........-............. 300 2 364 .....C........... 348 1 45 ...........A........G....------.........T............. 92 1 27 .....C........... 43 N A M S S I Y S D E Y Q L V I K L L R S A R I D R G I T Q S Query_1 198 TGCCATGTCATCTATTTATTCTGATGAATATCAGTTAGTTATAAAGTTACTGCGCTCTGCAAGAATTGATCGGGGGATTACTCAATCTCA 287 2 299 .......................................................................................... 210 1 93 .......................................................................................... 182 0 1 ...................................................................................... 86 Q L A K S L G K P Q S F V A K V E N G E R R L D V I E F A A Query_1 288 ACTGGCTAAATCGCTGGGCAAACCCCAGTCATTCGTTGCAAAGGTGGAGAATGGCGAACGTCGTTTGGATGTGATTGAATTTGCAGCTAT 377 2 209 .................A........................................................................ 120 2 1198 ..N......... 1187 1 183 .................A........................................................................ 272 0 87 .......................................................................................... 176 I A R L L S L D P A E I L N A V M K * H W K W I T D P N S S Query_1 378 TGCTCGCCTGCTTTCGCTTGATCCCGCAGAAATACTGAACGCAGTTATGAAGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTC 467 2 119 ....................................................................... 49 1 273 ........................................................................--..... 349 0 177 .................................................... 228 S V D K L A A A L E H H H H H H * D P A A N K A R K E A E L Query_1 468 CGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGC 557 2 46 ......................... 22 1 345 ........................................ 384 A A A T A E Q * L A * P L G A S K R V L R G F L L K Query_1 558 TGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAA 633 1 385 ............................................................................ 460 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1669218 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:51 PM Number of letters in database: 2,746 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96915.103 (IDP96915.1-143.F..pMCSG73)
MRKTSIFAAALLAISSIAHADERTTAVYALSSGIASIGSSALVSGIILSPVLVPVALITASVDKNKKQKTALLTTKDPKQNTVKMVLPLQVVEKVDLKTGDKLTLEKAPEGTGALLKKEDKVLAHMVNQEDASLSANQPLSAK
ATGCGTAAAACGTCCATCTTCGCCGCAGCATTGCTGGCAATTTCGTCCATCGCTCATGCCGATGAGCGTACCACCGCCGTCTATGCTCTCTCTTCGGGAATTGCCAGCATCGGCAGTTCGGCATTAGTGAGCGGTATTATTCTCTCGCCGGTCCTGGTTCCCGTCGCACTGATCACCGCTTCTGTCGACAAAAACAAAAAGCAAAAAACCGCGCTGCTGACCACCAAAGACCCTAAGCAGAACACCGTCAAAATGGTGCTGCCGCTGCAGGTTGTCGAAAAGGTCGATCTCAAAACCGGGGATAAGCTGACGCTGGAGAAAGCCCCGGAAGGCACCGGCGCATTGCTGAAAAAAGAGGATAAGGTTCTGGCCCACATGGTAAATCAAGAGGACGCGTCGCTCTCCGCTAACCAACCGCTGTCGGCGAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 439 total letters Query= IDP96915.103 Length=834 Score E Sequences producing significant alignments: (Bits) Value 0_wt 774 0.0 M R K T S I F A A A L L A I S S I A H A D E R T T A V Y A L Query_1 202 ATGCGTAAAACGTCCATCTTCGCCGCAGCATTGCTGGCAATTTCGTCCATCGCTCATGCCGATGAGCGTACCACCGCCGTCTATGCTCTC 291 0 1 .......................................................................................... 90 0 109 ........... 99 S S G I A S I G S S A L V S G I I L S P V L V P V A L I T A Query_1 292 TCTTCGGGAATTGCCAGCATCGGCAGTTCGGCATTAGTGAGCGGTATTATTCTCTCGCCGGTCCTGGTTCCCGTCGCACTGATCACCGCT 381 0 91 .......................................................................................... 180 0 42 ........... 32 S V D K N K K Q K T A L L T T K D P K Q N T V K M V L P L Q Query_1 382 TCTGTCGACaaaaacaaaaagcaaaaaaCCGCGCTGCTGACCACCAAAGACCCTAAGCAGAACACCGTCAAAATGGTGCTGCCGCTGCAG 471 0 181 .......................................................................................... 270 V V E K V D L K T G D K L T L E K A P E G T G A L L K K E D Query_1 472 GTTGTCGAAAAGGTCGATCTCAAAACCGGGGATAAGCTGACGCTGGAGAAAGCCCCGGAAGGCACCGGCGCATTGCTGAAAAAAGAGGAT 561 0 271 .......................................................................................... 360 K V L A H M V N Q E D A S L S A N Q P L S A K * H W K W I T Query_1 562 AAGGTTCTGGCCCACATGGTAAATCAAGAGGACGCGTCGCTCTCCGCTAACCAACCGCTGTCGGCGAAATAACATTGGAAGTGGATAACG 651 0 361 ..................................................................... 429 D P N S S S V D K L A A A L E H H H H H H * D P A A N K A R Query_1 652 GATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGA 741 K E A E L A A A T A E Q * L A * P L G A S K R V L R G F L Query_1 742 AAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCT 830 0 210 ........... 200 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 328400 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:51 PM Number of letters in database: 439 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96916.102 (IDP96916.1-906.F..pMCSG68)
MTRGQYPMSSVKSLLFSHNHHLLSVKGCEAGLDVLAFEGDEALSQPFRYRIEFTSADHAISKEMMLMKAASLTLQAPVAQGFGINVQQPVRVIQGVVTGFERLSTSRDETHYALTLQPRLALLNRSHQNAIYQDQSVPQIVEKILRERHGLRGQDFLFSLTKTYPRREQVMQYGEDDLRFITRLLGEVGIWFRFTADTRLHIDVAEFCDSQQGYEKGLTLPSVPPSGQQSAGVDAVWEMACRHRVVEQQVSTRDYNYREATADMNAQVDVTRGETTTFGEAYHWGDNYLTAGNVHDRHPAPESGAFYARLRHERYLNGQTRMQATTSCPTLCPGQVLKVTGGEEVAGEFADGVLVTAMHSHARRDADFAVEFAGIPDSPDVGYRPEPGARPVMAGTLPARVTSTRENDTYGHIDKHGRYRVNMLFDRARWETGFESLWVRQSRPYAGDTYGLHLPLLAGTEVAIGFEDGNPDRPYIAGVLHDSAHGDHVTIRNDKRNVLRTPANNKIRLDDERGKEHIKVSTEYGGKSQLNLGHLVDAEKQPRGEGFELRTDSWGAIRAQKGIFISADGQVQAQGQVLDMEPAVSNLAEAREQMMSISGDAQKATANPADLQAQITLLEQQLTDLKKSVLLVSAPEGIALTSGEHLQVSAGHNLIATAGKNADVSVVKNLFIGVGSALSVFVRKLGIRLIANQGPVQMQAQNDLMALLARKEISIVSTEDEIKIIAKKKLTLNGGGSYIRLDANAIESATAGEYRTKAGYYGRQESASEKVKMPVFPVMEPGEHSLRFAFPGADSVSQSMNWLINQPYSISDSAGKILSTGTVDTSGRFPKIVLPESDTLNLIIGRETWSKEELSVSSTEDDSMEDFEDEAPDADLHLSEMGFEDDRTSALTESMISTLLSLTKDS
TTGACGAGGGGACAATATCCGATGAGTAGCGTGAAATCGTTGTTGTTCAGCCATAACCACCACCTGCTTTCGGTGAAGGGATGTGAAGCCGGGCTTGATGTGCTGGCCTTCGAGGGCGATGAAGCCCTGAGCCAGCCGTTCCGCTACCGTATTGAATTCACCAGCGCAGACCATGCCATCAGCAAAGAGATGATGCTGATGAAGGCAGCCTCCCTGACGCTGCAGGCGCCGGTGGCTCAGGGCTTCGGCATCAATGTTCAGCAACCCGTGCGGGTGATCCAGGGCGTGGTGACGGGGTTTGAACGGCTCAGTACCTCCAGGGATGAAACGCACTACGCGCTGACGCTTCAGCCGCGCCTGGCGCTGCTTAACCGCTCGCACCAGAACGCCATTTACCAGGACCAGTCCGTTCCGCAGATTGTGGAAAAAATCCTCCGCGAGCGCCACGGCCTGCGCGGTCAGGATTTTCTGTTCTCGCTGACAAAAACGTATCCGCGACGCGAGCAGGTGATGCAGTACGGCGAGGATGACCTGCGCTTTATCACCCGGCTGCTGGGTGAGGTGGGGATCTGGTTTCGGTTTACCGCCGACACGCGGCTGCACATCGACGTCGCTGAGTTCTGCGACAGTCAGCAGGGCTATGAGAAGGGGCTGACGTTGCCTTCGGTCCCGCCGTCAGGGCAGCAGTCGGCCGGGGTGGACGCGGTGTGGGAGATGGCATGTCGCCACCGCGTCGTGGAGCAGCAGGTCAGTACCCGCGACTACAACTACCGCGAGGCGACGGCGGACATGAACGCGCAGGTGGATGTCACCCGCGGGGAGACCACTACCTTTGGTGAGGCTTACCACTGGGGAGATAACTACCTGACCGCGGGGAATGTCCATGACCGCCATCCGGCGCCGGAGTCCGGGGCGTTTTACGCCCGGCTTCGGCATGAGCGTTATCTTAACGGCCAGACCCGGATGCAGGCCACCACCAGCTGCCCGACGCTCTGCCCGGGACAGGTGCTGAAGGTCACCGGCGGAGAGGAGGTGGCCGGGGAGTTTGCTGATGGCGTGCTCGTCACGGCGATGCACAGCCACGCCCGGCGCGATGCCGACTTTGCGGTGGAGTTTGCCGGCATACCGGACAGCCCGGACGTCGGCTACCGGCCTGAGCCCGGCGCGCGTCCGGTGATGGCCGGGACGCTGCCGGCCCGCGTGACCAGCACCCGGGAAAACGACACCTACGGGCATATCGACAAACACGGACGCTACCGGGTGAATATGCTGTTTGACCGGGCGCGCTGGGAAACCGGTTTCGAGAGCCTGTGGGTCCGCCAGTCGCGGCCGTATGCCGGAGACACCTACGGCCTGCACCTGCCGCTGCTGGCCGGGACGGAGGTGGCGATTGGCTTTGAGGATGGTAACCCTGACCGGCCGTATATCGCGGGCGTGCTGCACGACTCGGCGCACGGCGACCACGTCACCATCCGCAATGACAAACGTAACGTCCTGCGCACCCCGGCCAACAACAAAATCCGCCTCGACGATGAGCGCGGAAAAGAGCATATCAAGGTCTCGACGGAGTACGGCGGTAAGAGCCAGCTGAACCTCGGCCATCTCGTGGATGCTGAAAAGCAGCCGCGCGGCGAAGGCTTTGAGCTGAGAACCGACAGCTGGGGCGCTATCCGGGCACAGAAAGGGATATTTATCAGCGCGGACGGACAGGTGCAGGCTCAGGGGCAGGTGCTGGATATGGAGCCGGCGGTCAGCAATCTGGCCGAAGCCCGGGAGCAGATGATGTCCATCTCCGGTGACGCGCAGAAGGCGACCGCGAACCCGGCCGATCTCCAGGCGCAAATTACCCTGCTGGAGCAGCAGCTGACAGACCTCAAAAAATCGGTACTGCTGGTCAGCGCGCCGGAGGGGATAGCGCTGACCAGCGGGGAGCACCTGCAGGTCTCAGCCGGGCATAACCTGATTGCCACAGCCGGAAAGAATGCAGACGTCAGCGTGGTGAAAAATCTGTTCATCGGGGTGGGCAGTGCGCTGAGCGTGTTTGTGCGAAAGCTTGGGATCAGGCTGATAGCGAATCAGGGACCGGTACAGATGCAGGCGCAGAATGACCTGATGGCGCTGCTTGCCCGGAAGGAAATCAGCATTGTCAGCACGGAGGATGAAATCAAAATCATCGCGAAGAAGAAGCTGACCCTGAATGGTGGGGGGAGTTATATCAGACTTGATGCAAATGCGATTGAATCTGCTACTGCAGGCGAATATCGCACGAAAGCAGGATATTACGGGCGTCAGGAAAGCGCTTCTGAAAAAGTCAAAATGCCTGTATTTCCTGTAATGGAGCCTGGAGAGCACAGTTTACGTTTTGCTTTTCCAGGTGCGGATAGTGTCAGCCAGAGTATGAACTGGCTGATAAATCAGCCGTACAGTATTTCTGATAGCGCTGGAAAAATTCTGTCTACCGGAACAGTAGACACTTCAGGTCGTTTTCCCAAAATAGTACTTCCTGAAAGCGATACATTAAATTTAATCATTGGCCGAGAAACTTGGAGTAAAGAGGAGCTTTCTGTCTCATCAACTGAAGACGACAGTATGGAGGATTTTGAGGATGAAGCACCTGATGCCGATCTTCATTTAAGTGAAATGGGTTTTGAAGATGATAGAACATCAGCATTGACTGAATCAATGATTTCAACTTTATTAAGTTTAACAAAGGATAGT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,154 total letters Query= IDP96916.102 Length=3123 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4902 0.0 2_08F_F2_IDP96916.102_T7term.seq 1912 0.0 1_02F_F2_IDP96916.102_T7S.seq 1880 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 56 AATTCCCCT-CTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTG 144 1 3 .........T................................................................................ 92 W S H P Q F E K G T E N L Y F Q S N A L T R G Q Y P M S S V Query_1 145 TGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCTTGACGAGGGGACAATATCCGATGAGTAGCGTG 234 0 1 ................................. 33 1 93 .........................................................A................................ 182 K S L L F S H N H H L L S V K G C E A G L D V L A F E G D E Query_1 235 AAATCGTTGTTGTTCAGCCATAACCACCACCTGCTTTCGGTGAAGGGATGTGAAGCCGGGCTTGATGTGCTGGCCTTCGAGGGCGATGAA 324 0 34 .......................................................................................... 123 0 246 ... 244 1 183 .......................................................................................... 272 1 395 ... 393 A L S Q P F R Y R I E F T S A D H A I S K E M M L M K A A S Query_1 325 GCCCTGAGCCAGCCGTTCCGCTACCGTATTGAATTCACCAGCGCAGACCATGCCATCAGCAAAGAGATGATGCTGATGAAGGCAGCCTCC 414 0 124 .......................................................................................... 213 0 1062 ..G..C............ 1045 0 243 ........... 233 1 273 .......................................................................................... 362 1 392 ........... 382 L T L Q A P V A Q G F G I N V Q Q P V R V I Q G V V T G F E Query_1 415 CTGACGCTGCAGGCGCCGGTGGCTCAGGGCTTCGGCATCAATGTTCAGCAACCCGTGCGGGTGATCCAGGGCGTGGTGACGGGGTTTGAA 504 0 214 .......................................................................................... 303 0 134 .............. 121 0 926 ........... 936 1 363 .......................................................................................... 452 1 283 .............. 270 R L S T S R D E T H Y A L T L Q P R L A L L N R S H Q N A I Query_1 505 CGGCTCAGTACCTCCAGGGATGAAACGCACTACGCGCTGACGCTTCAGCCGCGCCTGGCGCTGCTTAACCGCTCGCACCAGAACGCCATT 594 0 304 .......................................................................................... 393 0 2114 ........... 2124 2 735 ........... 725 1 453 .......................................................................................... 542 Y Q D Q S V P Q I V E K I L R E R H G L R G Q D F L F S L T Query_1 595 TACCAGGACCAGTCCGTTCCGCAGATTGTGGAAAAAATCCTCCGCGAGCGCCACGGCCTGCGCGGTCAGGATTTTCTGTTCTCGCTGACA 684 0 394 .......................................................................................... 483 1 543 .......................................................................................... 632 K T Y P R R E Q V M Q Y G E D D L R F I T R L L G E V G I W Query_1 685 AAAACGTATCCGCGACGCGAGCAGGTGATGCAGTACGGCGAGGATGACCTGCGCTTTATCACCCGGCTGCTGGGTGAGGTGGGGATCTGG 774 0 484 .......................................................................................... 573 1 633 .......................................................................................... 722 F R F T A D T R L H I D V A E F C D S Q Q G Y E K G L T L P Query_1 775 TTTCGGTTTACCGCCGACACGCGGCTGCACATCGACGTCGCTGAGTTCTGCGACAGTCAGCAGGGCTATGAGAAGGGGCTGACGTTGCCT 864 0 574 .......................................................................................... 663 1 723 .......................................................................................... 812 S V P P S G Q Q S A G V D A V W E M A C R H R V V E Q Q V S Query_1 865 TCGGTCCCGCCGTCAGGGCAGCAGTCGGCCGGGGTGGACGCGGTGTGGGAGATGGCATGTCGCCACCGCGTCGTGGAGCAGCAGGTCAGT 954 0 664 .......................................................................................... 753 0 1853 ........... 1863 2 997 ........... 987 1 813 .......................................................................................... 902 T R D Y N Y R E A T A D M N A Q V D V T R G E T T T F G E A Query_1 955 ACCCGCGACTACAACTACCGCGAGGCGACGGCGGACATGAACGCGCAGGTGGATGTCACCCGCGGGGAGACCACTACCTTTGGTGAGGCT 1044 0 754 .......................................................................................... 843 1 903 .............................-............................-....-..............-.......A... 988 Y H W G D N Y L T A G N V H D R H P A P E S G A F Y A R L Query_1 1045 TACCACTGGGGAG-ATAACTACCTGACCGCGGGGAATGTCCATGACCGCCATCCGGCGCCGGAGTCCGGGGCGTTTTACGCCCGGCTTCG 1133 0 844 .............-............................................................................ 932 0 905 ............ 894 0 241 ....... 247 1 989 .............A................N.......................N...-.N......-N..N...........N...... 1076 1 390 ....... 396 R H E R Y L N G Q T R M Q A T T S C P T L C P G Q V L K V T Query_1 1134 GCATGAGCGTTATCTTAACGGCCAGACCCGGATGCAGGCCACCACCAGCTGCCCGACGCTCTGCCCGGGACAGGTGCTGAAGGTCACCGG 1223 0 933 .......................................................................................... 1022 0 248 .... 251 1 1077 ........-NN........NNNN.---..NN....-N.NN..--A............NN......--.NNN.......N... 1149 1 397 .... 400 G G E E V A G E F A D G V L V T A M H S H A R R D A D F A V Query_1 1224 CGGAGAGGAGGTGGCCGGGGAGTTTGCTGATGGCGTGCTCGTCACGGCGATGCACAGCCACGCCCGGCGCGATGCCGACTTTGCGGTGGA 1313 0 1023 .......................................................................................... 1112 0 186 ............A..G.. 169 1 335 ............A..G.. 318 E F A G I P D S P D V G Y R P E P G A R P V M A G T L P A R Query_1 1314 GTTTGCCGGCATACCGGACAGCCCGGACGTCGGCTACCGGCCTGAGCCCGGCGCGCGTCCGGTGATGGCCGGGACGCTGCCGGCCCGCGT 1403 0 1113 .......................................................................................... 1202 0 1363 ...C..C........... 1380 V T S T R E N D T Y G H I D K H G R Y R V N M L F D R A R W Query_1 1404 GACCAGCACCCGGGAAAACGACACCTACGGGCATATCGACAAACACGGACGCTACCGGGTGAATATGCTGTTTGACCGGGCGCGCTGGGA 1493 0 1203 .......................................................................................... 1292 0 1342 ........... 1352 E T G F E S L W V R Q S R P Y A G D T Y G L H L P L L A G T Query_1 1494 AACCGGTTTCGAGAGCCTGTGGGTCCGCCAGTCGCGGCCGTATGCCGGAGACACCTACGGCCTGCACCTGCCGCTGCTGGCCGGGACGGA 1583 0 1293 .......................................................................................... 1382 0 1171 ...G..A........... 1188 0 1222 ........... 1232 E V A I G F E D G N P D R P Y I A G V L H D S A H G D H V T Query_1 1584 GGTGGCGATTGGCTTTGAGGATGGTAACCCTGACCGGCCGTATATCGCGGGCGTGCTGCACGACTCGGCGCACGGCGACCACGTCACCAT 1673 0 1383 .......................................................................................... 1472 I R N D K R N V L R T P A N N K I R L D D E R G K E H I K V Query_1 1674 CCGCAATGACAAACGTAACGTCCTGCGCACCCCGGCCAACAACAAAATCCGCCTCGACGATGAGCGCGGAAAAGAGCATATCAAGGTCTC 1763 0 1473 .......................................................................................... 1562 S T E Y G G K S Q L N L G H L V D A E K Q P R G E G F E L R Query_1 1764 GACGGAGTACGGCGGTAAGAGCCAGCTGAACCTCGGCCATCTCGTGGATGCTGAAAAGCAGCCGCGCGGCGAAGGCTTTGAGCTGAGAAC 1853 0 1563 .......................................................................................... 1652 T D S W G A I R A Q K G I F I S A D G Q V Q A Q G Q V L D M Query_1 1854 CGACAGCTGGGGCGCTATCCGGGCACAGAAAGGGATATTTATCAGCGCGGACGGACAGGTGCAGGCTCAGGGGCAGGTGCTGGATATGGA 1943 0 1653 .......................................................................................... 1742 0 1362 ........... 1352 2 1150 ..........-...ANN--..............-...N.....A....... 1104 E P A V S N L A E A R E Q M M S I S G D A Q K A T A N P A D Query_1 1944 GCCGGCGGTCAGCAATCTGGCCGAAGCCCGGGAGCAGATGATGTCCATCTCCGGTGACGCGCAGAA-GGCGACCGCGAACCCGGCCGATC 2032 0 1743 ..................................................................-....................... 1831 2 1103 N...-.........TN.....N..G--.......................................A....N......N...N....... 1017 L Q A Q I T L L E Q Q L T D L K K S V L L V S A P E G I A L Query_1 2033 TCCAGGCGCAAATTACCCTGCTGGAGCAGCAGCTGACAGACCTCAAAAAA-TCGGTACTGCTGGTCAGCGCGCCGGAGGGGATAGCGCTG 2121 0 1832 ..................................................-....................................... 1920 0 1901 ...... 1896 0 1926 ............ 1915 0 737 ........... 747 2 1016 N..........-.....-................................A....................................... 929 2 923 ............ 934 2 948 ...... 953 1 886 ........... 896 T S G E H L Q V S A G H N L I A T A G K N A D V S V V K N L Query_1 2122 ACCAGCGGGGAGCACCTGCAGGTCTCAGCCGGGCATAACCTGATTGCCACAGCCGGAAAGAATGCAGACGTCAGCGTGGTGAAAAATCTG 2211 0 1921 .......................................................................................... 2010 0 1895 ...... 1890 2 928 .......................................................................................... 839 2 954 ...... 959 F I G V G S A L S V F V R K L G I R L I A N Q G P V Q M Q A Query_1 2212 TTCATCGGGGTGGGCAGTGCGCTGAGCGTGTTTGTGCGAAAGCTTGGGATCAGGCTGATAGCGAATCAGGGACCGGTACAGATGCAGGCG 2301 0 2011 .......................................................................................... 2100 2 838 .......................................................................................... 749 Q N D L M A L L A R K E I S I V S T E D E I K I I A K K K L Query_1 2302 CAGAATGACCTGATGGCGCTGCTTGCCCGGAAGGAAATCAGCATTGTCAGCACGGAGGATGAAATCAAAATCATCGCGAAGAAGAAGCTG 2391 0 2101 .......................................................................................... 2190 2 748 .......................................................................................... 659 1 508 ........... 518 T L N G G G S Y I R L D A N A I E S A T A G E Y R T K A G Y Query_1 2392 ACCCTGAATGGTGGGGGGAGTTATATCAGACTTGATGCAAATGCGATTGAATCTGCTACTGCAGGCGAATATCGCACGAAAGCAGGATAT 2481 0 2191 .......................................................................................... 2280 2 658 .......................................................................................... 569 Y G R Q E S A S E K V K M P V F P V M E P G E H S L R F A F Query_1 2482 TACGGGCGTCAGGAAAGCGCTTCTGAAAAAGTCAAAATGCCTGTATTTCCTGTAATGGAGCCTGGAGAGCACAGTTTACGTTTTGCTTTT 2571 0 2281 .......................................................................................... 2370 2 568 .......................................................................................... 479 P G A D S V S Q S M N W L I N Q P Y S I S D S A G K I L S T Query_1 2572 CCAGGTGCGGATAGTGTCAGCCAGAGTATGAACTGGCTGATAAATCAGCCGTACAGTATTTCTGATAGCGCTGGAAAAATTCTGTCTACC 2661 0 2371 .......................................................................................... 2460 2 478 .......................................................................................... 389 G T V D T S G R F P K I V L P E S D T L N L I I G R E T W S Query_1 2662 GGAACAGTAGACACTTCAGGTCGTTTTCCCAAAATAGTACTTCCTGAAAGCGATACATTAAATTTAATCATTGGCCGAGAAACTTGGAGT 2751 0 2461 .......................................................................................... 2550 0 2528 ............ 2517 0 1224 ........... 1214 2 388 .......................................................................................... 299 2 321 ............ 332 K E E L S V S S T E D D S M E D F E D E A P D A D L H L S E Query_1 2752 AAAGAGGAGCTTTCTGTCTCATCAACTGAAGACGACAGTATGGAGGATTTTGAGGATGAAGCACCTGATGCCGATCTTCATTTAAGTGAA 2841 0 2551 .......................................................................................... 2640 2 298 .......................................................................................... 209 M G F E D D R T S A L T E S M I S T L L S L T K D S * H W K Query_1 2842 ATGGGTTTTGAAGATGATAGAACATCAGCATTGACTGAATCAATGATTTCAACTTTATTAAGTTTAACAAAGGATAGTTAACATTGGAAG 2931 0 2641 .............................................................................. 2718 2 208 .....-.................................................................................... 120 W I T D P N S S S V D K L A A A L E H H H H H H * D P A A N Query_1 2932 TGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAAC 3021 2 119 .......................................................................................... 30 K A R K Query_1 3022 AAAG-CCCGAAAG 3033 2 29 ....C........ 17 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 15835500 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:51 PM Number of letters in database: 5,154 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96917.103 (IDP96917.1-343.F..pMCSG73)
MQLKKIFISGFKSISNQNPQTIDLEHELTTFIGHNGTGKSTAMEALNKLFSVDNSLRSLSINDFHNADDGKDEKTRSLIIETWFSFPKPKEGKISIPPLIEHLTIDKSDGNILFRVRLEGKLSFEANPMGDIDEDIWVVSTSDEVVEESSKQKLSAAVRNAIQVSYVPANRDPLLQLKYSSKAVLGRLLKAIEWVGGSQEVLEEQAAKLNALTKSNPALNEIAAAINASWGNIYKGRYLSQASLNFPLANIDEILKLIQLQFNPDETGNTINVDRLSDGQKSLVYFSLIKAMFDIDKKTRELISSGAASNFNPEKMRLPIFSMISLEEPENHLSPHYLGRIIS
ATGCAACTTAAGAAAATTTTTATCTCCGGCTTTAAAAGCATTTCCAATCAAAATCCCCAAACCATTGACCTTGAGCATGAATTAACCACGTTTATTGGCCATAATGGAACTGGAAAGAGTACAGCTATGGAGGCGCTTAATAAGCTTTTTTCGGTGGATAATTCCCTTCGAAGCTTATCAATAAATGACTTCCATAATGCGGATGATGGGAAAGATGAAAAAACCAGAAGCTTAATTATAGAAACTTGGTTTAGCTTTCCTAAACCCAAAGAAGGCAAAATTTCCATCCCTCCGTTAATTGAACATCTTACCATTGATAAATCTGATGGGAATATTCTTTTTAGGGTGCGATTAGAGGGGAAACTTTCTTTTGAAGCAAATCCCATGGGGGATATTGACGAAGATATCTGGGTAGTAAGTACCAGCGACGAGGTTGTTGAAGAGAGCAGTAAGCAGAAGCTTTCCGCCGCAGTAAGAAACGCCATTCAAGTGAGTTATGTTCCAGCCAACCGAGATCCTTTATTGCAATTAAAATATTCATCGAAAGCGGTGCTTGGACGCTTATTGAAAGCGATTGAATGGGTTGGAGGGAGCCAGGAAGTATTAGAGGAGCAAGCAGCTAAGTTAAACGCATTAACGAAGAGTAATCCTGCATTAAATGAGATTGCGGCGGCGATTAACGCCAGTTGGGGGAATATCTACAAGGGGCGTTATTTATCTCAGGCGTCGCTGAATTTTCCACTCGCTAATATCGATGAAATACTAAAATTAATACAACTTCAATTTAACCCTGATGAAACCGGCAACACGATTAATGTAGACCGACTAAGCGATGGGCAAAAATCATTGGTCTATTTTTCTCTTATCAAGGCAATGTTTGATATTGATAAGAAGACTAGGGAGTTGATTTCCTCAGGCGCGGCTTCTAATTTTAATCCTGAAAAAATGAGATTGCCTATTTTTAGTATGATCTCTCTGGAAGAGCCTGAAAACCATCTTTCTCCTCATTACTTAGGTAGAATAATTAGT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,509 total letters Query= IDP96917.103 Length=1434 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1856 0.0 2_12D_D6_IDP96917.103_T7_Term.seq 1831 0.0 1_06D_D6_IDP96917.103_v73F.seq 1799 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 2 1103 ......... 1095 2 1148 ........... 1138 2 1148 ........... 1138 1 42 ...........A........G....------....................... 89 1 24 .....C........... 40 A M Q L K K I F I S G F K S I S N Q N P Q T I D L E H E L T Query_1 199 GCCATGCAACTTAAGAAAATTTTTATCTCCGGCTTTAAAAGCATTTCCAATCAAAATCCCCAAACCATTGACCTTGAGCATGAATTAACC 288 0 1 ....................................................................................... 87 2 1094 N.-.....-....-.........AT....N.....-.........NG........-.......N.......................N.. 1010 1 90 .......................................................................................... 179 T F I G H N G T G K S T A M E A L N K L F S V D N S L R S L Query_1 289 ACGTTTATTGGCCATAATGGAACTGGAAAGAGTACAGCTATGGAGGCGCTTAATAAGCTTTTTTCGGTGGATAATTCCCTTCGAAGCTTA 378 0 88 .......................................................................................... 177 2 1009 .....................................................C.................................... 920 1 180 .....................................................C.................................... 269 S I N D F H N A D D G K D E K T R S L I I E T W F S F P K P Query_1 379 TCAATAAATGACTTCCATAATGCGGATGATGGGAAAGATGAAAAAACCAGAAGCTTAATTATAGAAACTTGGTTTAGCTTTCCTAAACCC 468 0 178 .......................................................................................... 267 2 919 .......................................................................................... 830 1 270 .......................................................................................... 359 K E G K I S I P P L I E H L T I D K S D G N I L F R V R L Query_1 469 AAAGAAGGCAAAATTTCCATCCCTCC-GTTAATTGAACATCTTACCATTGATAAATCTGATGGGAATATTCTTTTTAGGGTGCGATTAGA 557 0 268 ..........................-............................................................... 356 2 829 ..........................C............................................................... 740 1 360 ..........................-............................................................... 448 E G K L S F E A N P M G D I D E D I W V V S T S D E V V E E Query_1 558 GGGGAAACTTTCTTTTGAAGCAAATCCCATGGGGGATATTGACGAAGATATCTGGGTAGTAAGTACCAGCGACGAGGTTGTTGAAGAGAG 647 0 357 .......................................................................................... 446 2 739 .......................................................................................... 650 1 449 .......................................................................................... 538 S S K Q K L S A A V R N A I Q V S Y V P A N R D P L L Q L K Query_1 648 CAGTAAGCAGAAGCTTTCCGCCGCAGTAAGAAACGCCATTCAAGTGAGTTATGTTCCAGCCAACCGAGATCCTTTATTGCAATTAAAATA 737 0 447 .......................................................................................... 536 2 649 .......................................................................................... 560 1 539 .......................................................................................... 628 Y S S K A V L G R L L K A I E W V G G S Q E V L E E Q A A K Query_1 738 TTCATCGAAAGCGGTGCTTGGACGCTTATTGAAAGCGATTGAATGGGTTGGAGGGAGCCAGGAAGTATTAGAGGAGCAAGCAGCTAAGTT 827 0 537 .......................................................................................... 626 2 559 .......................................................................................... 470 1 629 .......................................................................................... 718 L N A L T K S N P A L N E I A A A I N A S W G N I Y K G R Y Query_1 828 AAACGCATTAACGAAGAGTAATCCTGCATTAAATGAGATTGCGGCGGCGATTAACGCCAGTTGGGGGAATATCTACAAGGGGCGTTATTT 917 0 627 .......................................................................................... 716 0 933 ........A.A-............ 955 2 469 .......................................................................................... 380 2 163 ........A.A-............ 141 1 719 .......................................................................................... 808 1 1025 ........A.--............ 1046 L S Q A S L N F P L A N I D E I L K L I Q L Q F N P D E T G Query_1 918 ATCTCAGGCGTCGCTGAATTTTCCACTCGCTAATATCGATGAAATACTAAAATTAATACAACTTCAATTTAACCCTGATGAAACCGGCAA 1007 0 717 .......................................................................................... 806 2 379 .......................................................................................... 290 1 809 .........................................-................................................ 897 N T I N V D R L S D G Q K S L V Y F S L I K A M F D I D K K Query_1 1008 CACGATTAATGTAGACCGACTAAGCGATGGGCAAAAATCATTGGTCTA-TTTTTCTC-TTATCAAGGCAATGTTTGATATTGATAAGAAG 1095 0 807 ................................................-........-................................ 894 2 289 ................................................-........-................................ 202 1 898 ................................................T........T................................ 987 T R E L I S S G A A S N F N P E K M R L P I F S M I S L E Query_1 1096 ACTAGGGAGTTGATTTCCTCAGGCGCGGCTTCTAATTTTAATCCTGAAA-AAATGAGATTGCCT-ATTTTTAGTATGATCTC-TCTGGAA 1182 0 895 .................................................-..............-.................-....... 981 0 645 ........C.TT............ 668 2 201 .................................................-..............-.................-....... 115 2 451 ........C.TT............ 428 1 988 ....-..N......................................-..-..............N..............N..T.....-N 1073 1 737 ........C.TT............ 760 E P E N H L S P H Y L G R I I S * H W K W I T D P N S S S Query_1 1183 GAGCCTGAAAACCA-TCTTT--CTCCTCATTACTTAGGTAGAATAATTAGTTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCC 1269 0 982 ..............-.....--............................. 1029 2 114 ..............-.....--.................................................--..... 42 1 1074 ..............T.....TCN......-.N...-NN.....AT.AN.NN......N.N..... 1136 V D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 1270 GTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCC--GAAAGGAA 1347 2 46 .....................CNA....... 16 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4907698 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:51 PM Number of letters in database: 3,509 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96920.103 (IDP96920.1-73.F..pMCSG73)
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGACCATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATGATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,574 total letters Query= IDP96920.103 Length=624 Score E Sequences producing significant alignments: (Bits) Value 2_12E_E6_IDP96920.103_T7_Term.seq 500 3e-145 1_06E_E6_IDP96920.103_v73F.seq 493 4e-143 0_wt 396 8e-114 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAG-TTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAA 197 2 334 ...........A...-.....G....------...................... 288 2 352 .....C........... 336 1 44 ...........A...G.....G....------...................... 91 1 26 .....C........... 42 N A M R T V N Y S E A R Q N L A D V L E S A V T G V P V T I Query_1 198 TGCCATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGACCATTAC 287 2 287 .......................................................................................... 198 1 92 .......................................................................................... 181 0 1 ...................................................................................... 86 T R R G H K S A V I I S A E E F E R Y Q A A R M D D E F A A Query_1 288 CCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATGATGAGTTCGCGGCTAT 377 2 197 .......................................................................................... 108 1 182 .......................................................................................... 271 0 87 .......................................................................................... 176 I M A V H G D E I R E L A D K * H W K W I T D P N S S S V D Query_1 378 CATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAA 467 2 107 ...............................................................--..... 40 1 272 ...............................................................--..... 339 0 177 ........................................... 219 K L A A A L E H H H H H H * D P A A N K A R K E A E L A A A Query_1 468 GCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCG-AAAGGAAGCTGAGTTGGCTGCTGCCA 556 2 44 ......................A....... 15 1 335 ......................-.......................... 382 T A E Q * L A * P L G A S K R V L R G F L L K Query_1 557 CCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAA 624 1 383 .................................................................... 450 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1540161 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:51 PM Number of letters in database: 2,574 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96921.103 (IDP96921.15-128.P(128)..pMCSG73)
SRPVRILRIAGLYPGQLSRTTSQCPHADGFNASNSSFDTVPFFINFRGSAMTTSTPPAPYFTLQINGLDYLRRIRDVHPKKGDSFLACAIAALNGPTTKPEYRYFDIRVSGDEA
TCACGGCCGGTTCGCATTCTGCGGATCGCCGGGCTATATCCAGGACAGCTTTCTCGCACAACGAGTCAATGTCCTCATGCGGATGGTTTCAATGCCAGTAACAGCTCCTTCGACACTGTTCCCTTTTTTATCAACTTCAGGGGGTCAGCCATGACAACCTCAACGCCCCCTGCGCCGTACTTTACCCTGCAAATTAACGGTCTGGACTATCTCCGCCGCATCCGCGACGTGCACCCTAAAAAAGGCGACAGCTTCCTTGCGTGCGCCATCGCGGCACTGAACGGTCCTACCACCAAACCGGAATATCGCTATTTTGATATTCGGGTTTCAGGCGATGAGGCG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,699 total letters Query= IDP96921.103 Length=747 Score E Sequences producing significant alignments: (Bits) Value 2_12F_F6_IDP96921.103_T7_Term.seq 722 0.0 1_06F_F6_IDP96921.103_v73F.seq 722 0.0 0_wt 618 2e-180 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 2 457 ...........A........G....------....................... 410 2 475 .....C........... 459 1 45 ...........A........G....------....................... 92 1 29 ...C........... 43 A S R P V R I L R I A G L Y P G Q L S R T T S Q C P H A D G Query_1 199 GCCTCACGGCCGGTTCGCATTCTGCGGATCGCCGGGCTATATCCAGGACAGCTTTCTCGCACAACGAGTCAATGTCCTCATGCGGATGGT 288 2 409 .......................................................................................... 320 1 93 .......................................................................................... 182 0 1 ....................................................................................... 87 F N A S N S S F D T V P F F I N F R G S A M T T S T P P A P Query_1 289 TTCAATGCCAGTAACAGCTCCTTCGACACTGTTCCCTTTTTTATCAACTTCAGGGGGTCAGCCATGACAACCTCAACGCCCCCTGCGCCG 378 2 319 .......................................................................................... 230 1 183 .......................................................................................... 272 0 88 .......................................................................................... 177 Y F T L Q I N G L D Y L R R I R D V H P K K G D S F L A C A Query_1 379 TACTTTACCCTGCAAATTAACGGTCTGGACTATCTCCGCCGCATCCGCGACGTGCACCCTAAAAAAGGCGACAGCTTCCTTGCGTGCGCC 468 2 229 .......................................................................................... 140 1 273 .......................................................................................... 362 0 178 .......................................................................................... 267 I A A L N G P T T K P E Y R Y F D I R V S G D E A * H W K W Query_1 469 ATCGCGGCACTGAACGGTCCTACCACCAAACCGGAATATCGCTATTTTGATATTCGGGTTTCAGGCGATGAGGCGTAACATTGGAAGTGG 558 2 139 .......................................................................................... 50 1 363 .......................................................................................... 452 0 268 ........................................................................... 342 I T D P N S S S V D K L A A A L E H H H H H H * D P A A N K Query_1 559 ATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAA 648 2 49 .....--..... 40 2 44 ................. 28 1 453 .....--..... 462 1 458 ................. 474 A R K E A E L A A A T A E Q * L A * P L G A S K R V L R G Query_1 649 GCCCG-AAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTT 737 2 27 .....A........ 14 1 475 .....-.................................................................................... 563 F L L K Query_1 738 TTTGCTGAAA 747 1 564 .......... 573 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1942728 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:51 PM Number of letters in database: 2,699 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96922.102 (IDP96922.1-538.F..pMCSG68)
MNRMSIVILWALALLVLQPALAAEPRQQPTAREQARTVTIFHQPVVMLQATFGQTTPEERVLRTRSALRAFTEDDIRQPLRVVPVIRYGQPGRLLLMNGKPVLLLSQADLDEGDDLTLDQAAQRVLARMEAQRTSLQEQFNNRYLFISAGKALAGALLLALFHYGAFRAWRRVRRFFLLQILEKRSAIPQHWRRYLGNIEVRLYAVLVIFLGMLACYLWLSWAFSLFPWTRVWSESLGDWSLGVIRDLSLSIVASLPGLMIVVLIFLLTWLIIRLVKVVLDQVAAGRIQVPGIHPETVSATRRLISVVIWLFALSAAYPFLPGANSLAFKGISVFFGLMLTLGSTGVMTHAMSGLVLIYSRALRKGDWIRLADNEGQVSEIGVLATKILTRENYIVTVPNAVVVSGKIINLSAESADGGVNLTTSVTIGYDTPWRQVHALLELAARRTPGIDQQIAPVVRKLGLLDWYTSYELQVRLLPTTKLPDGRNALHSSIIDVFNEFGVQIMSPNFVMQPKAAVVVPQEAWYAAPAVAPQEPEK
ATGAACAGGATGAGCATCGTTATCCTCTGGGCGCTGGCGCTGCTGGTGCTCCAGCCGGCGCTGGCCGCCGAACCGCGACAGCAGCCAACCGCGCGCGAGCAGGCACGCACGGTCACGATTTTCCACCAACCTGTCGTTATGTTGCAGGCCACCTTTGGCCAGACCACGCCCGAAGAGCGCGTTTTGCGCACCCGCAGCGCCCTGCGCGCCTTCACCGAAGATGATATTCGCCAGCCGCTGCGGGTCGTGCCGGTTATCCGCTATGGTCAGCCAGGCAGACTGTTGCTGATGAACGGCAAACCGGTGCTGCTGCTGAGCCAGGCGGATCTCGACGAGGGCGATGATTTAACCCTCGACCAGGCAGCCCAGCGGGTGCTGGCGCGTATGGAAGCCCAGCGCACCAGCCTCCAGGAGCAGTTCAACAACCGCTATCTGTTCATCTCCGCCGGTAAAGCCCTGGCCGGGGCGCTGCTGCTGGCGCTGTTCCACTACGGTGCCTTCCGCGCCTGGCGGCGGGTGCGACGCTTTTTCCTGCTGCAGATCCTCGAAAAACGTAGCGCCATACCCCAGCACTGGCGGCGCTATCTCGGCAATATTGAAGTTCGACTCTATGCTGTGCTGGTCATCTTTCTCGGCATGCTGGCCTGTTATCTCTGGCTGAGCTGGGCCTTCAGCCTGTTTCCCTGGACGCGGGTGTGGAGCGAATCGCTGGGCGACTGGTCGCTGGGGGTGATCCGCGACCTCTCTCTGTCGATTGTCGCCTCCCTGCCGGGGCTGATGATCGTGGTGCTTATTTTCCTTCTCACCTGGCTTATCATTCGCCTGGTGAAGGTGGTTCTCGACCAGGTCGCCGCCGGCCGCATTCAGGTTCCCGGCATTCATCCGGAGACCGTTAGCGCCACCCGTCGACTTATCTCGGTGGTCATCTGGTTGTTTGCCCTCTCCGCCGCCTACCCGTTCCTTCCCGGCGCCAACTCTCTCGCCTTCAAAGGCATTAGCGTGTTCTTCGGCCTGATGCTGACCCTCGGGTCAACCGGAGTGATGACCCATGCGATGAGCGGCCTGGTGCTGATCTACTCTCGGGCGCTGCGCAAAGGCGACTGGATCCGCCTCGCCGATAATGAGGGTCAGGTCAGCGAGATTGGCGTACTGGCGACCAAAATTCTCACCCGCGAAAATTACATCGTTACCGTCCCGAACGCGGTGGTGGTGAGCGGGAAAATTATTAATCTCAGCGCCGAAAGCGCCGACGGTGGAGTCAACCTCACCACCAGCGTCACCATCGGCTACGATACGCCGTGGCGTCAGGTCCATGCGCTGCTGGAGCTGGCGGCGAGACGCACCCCGGGGATCGATCAGCAAATTGCGCCGGTGGTGCGTAAGCTGGGATTGCTTGACTGGTACACCTCGTATGAGCTGCAGGTGCGCTTACTCCCCACAACCAAGCTGCCGGATGGCCGCAACGCATTGCATAGCAGCATTATTGATGTGTTTAACGAATTCGGCGTGCAGATTATGTCGCCGAACTTTGTGATGCAGCCAAAGGCGGCGGTAGTGGTACCGCAGGAAGCCTGGTACGCCGCCCCGGCCGTCGCGCCGCAGGAGCCTGAGAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 1,624 total letters Query= IDP96922.102 Length=2019 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2911 0.0 M N R M S I V I L W A L A L L V L Q P A L A A E P R Q Q P T Query_1 202 ATGAACAGGATGAGCATCGTTATCCTCTGGGCGCTGGCGCTGCTGGTGCTCCAGCCGGCGCTGGCCGCCGAACCGCGACAGCAGCCAACC 291 0 1 .......................................................................................... 90 A R E Q A R T V T I F H Q P V V M L Q A T F G Q T T P E E R Query_1 292 GCGCGCGAGCAGGCACGCACGGTCACGATTTTCCACCAACCTGTCGTTATGTTGCAGGCCACCTTTGGCCAGACCACGCCCGAAGAGCGC 381 0 91 .......................................................................................... 180 V L R T R S A L R A F T E D D I R Q P L R V V P V I R Y G Q Query_1 382 GTTTTGCGCACCCGCAGCGCCCTGCGCGCCTTCACCGAAGATGATATTCGCCAGCCGCTGCGGGTCGTGCCGGTTATCCGCTATGGTCAG 471 0 181 .......................................................................................... 270 P G R L L L M N G K P V L L L S Q A D L D E G D D L T L D Q Query_1 472 CCAGGCAGACTGTTGCTGATGAACGGCAAACCGGTGCTGCTGCTGAGCCAGGCGGATCTCGACGAGGGCGATGATTTAACCCTCGACCAG 561 0 271 .......................................................................................... 360 A A Q R V L A R M E A Q R T S L Q E Q F N N R Y L F I S A G Query_1 562 GCAGCCCAGCGGGTGCTGGCGCGTATGGAAGCCCAGCGCACCAGCCTCCAGGAGCAGTTCAACAACCGCTATCTGTTCATCTCCGCCGGT 651 0 361 .......................................................................................... 450 K A L A G A L L L A L F H Y G A F R A W R R V R R F F L L Q Query_1 652 AAAGCCCTGGCCGGGGCGCTGCTGCTGGCGCTGTTCCACTACGGTGCCTTCCGCGCCTGGCGGCGGGTGCGACGCTTTTTCCTGCTGCAG 741 0 451 .......................................................................................... 540 I L E K R S A I P Q H W R R Y L G N I E V R L Y A V L V I F Query_1 742 ATCCTCGAAAAACGTAGCGCCATACCCCAGCACTGGCGGCGCTATCTCGGCAATATTGAAGTTCGACTCTATGCTGTGCTGGTCATCTTT 831 0 541 .......................................................................................... 630 L G M L A C Y L W L S W A F S L F P W T R V W S E S L G D W Query_1 832 CTCGGCATGCTGGCCTGTTATCTCTGGCTGAGCTGGGCCTTCAGCCTGTTTCCCTGGACGCGGGTGTGGAGCGAATCGCTGGGCGACTGG 921 0 631 .......................................................................................... 720 S L G V I R D L S L S I V A S L P G L M I V V L I F L L T W Query_1 922 TCGCTGGGGGTGATCCGCGACCTCTCTCTGTCGATTGTCGCCTCCCTGCCGGGGCTGATGATCGTGGTGCTTATTTTCCTTCTCACCTGG 1011 0 721 .......................................................................................... 810 L I I R L V K V V L D Q V A A G R I Q V P G I H P E T V S A Query_1 1012 CTTATCATTCGCCTGGTGAAGGTGGTTCTCGACCAGGTCGCCGCCGGCCGCATTCAGGTTCCCGGCATTCATCCGGAGACCGTTAGCGCC 1101 0 811 .......................................................................................... 900 T R R L I S V V I W L F A L S A A Y P F L P G A N S L A F K Query_1 1102 ACCCGTCGACTTATCTCGGTGGTCATCTGGTTGTTTGCCCTCTCCGCCGCCTACCCGTTCCTTCCCGGCGCCAACTCTCTCGCCTTCAAA 1191 0 901 .......................................................................................... 990 G I S V F F G L M L T L G S T G V M T H A M S G L V L I Y S Query_1 1192 GGCATTAGCGTGTTCTTCGGCCTGATGCTGACCCTCGGGTCAACCGGAGTGATGACCCATGCGATGAGCGGCCTGGTGCTGATCTACTCT 1281 0 991 .......................................................................................... 1080 R A L R K G D W I R L A D N E G Q V S E I G V L A T K I L T Query_1 1282 CGGGCGCTGCGCAAAGGCGACTGGATCCGCCTCGCCGATAATGAGGGTCAGGTCAGCGAGATTGGCGTACTGGCGACCAAAATTCTCACC 1371 0 1081 .......................................................................................... 1170 R E N Y I V T V P N A V V V S G K I I N L S A E S A D G G V Query_1 1372 CGCGAAAATTACATCGTTACCGTCCCGAACGCGGTGGTGGTGAGCGGGAAAATTATTAATCTCAGCGCCGAAAGCGCCGACGGTGGAGTC 1461 0 1171 .......................................................................................... 1260 N L T T S V T I G Y D T P W R Q V H A L L E L A A R R T P G Query_1 1462 AACCTCACCACCAGCGTCACCATCGGCTACGATACGCCGTGGCGTCAGGTCCATGCGCTGCTGGAGCTGGCGGCGAGACGCACCCCGGGG 1551 0 1261 .......................................................................................... 1350 I D Q Q I A P V V R K L G L L D W Y T S Y E L Q V R L L P T Query_1 1552 ATCGATCAGCAAATTGCGCCGGTGGTGCGTAAGCTGGGATTGCTTGACTGGTACACCTCGTATGAGCTGCAGGTGCGCTTACTCCCCACA 1641 0 1351 .......................................................................................... 1440 T K L P D G R N A L H S S I I D V F N E F G V Q I M S P N F Query_1 1642 ACCAAGCTGCCGGATGGCCGCAACGCATTGCATAGCAGCATTATTGATGTGTTTAACGAATTCGGCGTGCAGATTATGTCGCCGAACTTT 1731 0 1441 .......................................................................................... 1530 V M Q P K A A V V V P Q E A W Y A A P A V A P Q E P E K * H Query_1 1732 GTGATGCAGCCAAAGGCGGCGGTAGTGGTACCGCAGGAAGCCTGGTACGCCGCCCCGGCCGTCGCGCCGCAGGAGCCTGAGAAATAACAT 1821 0 1531 .................................................................................... 1614 W K W I T D P N S S S V D K L A A A L E H H H H H H * D P A Query_1 1822 TGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCT 1911 A N K A R K E A E L A A Query_1 1912 GCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG 1948 0 89 ........... 79 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3156728 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:51 PM Number of letters in database: 1,624 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96922.105 (IDP96922.361-538.P(538)..pMCSG73)
RALRKGDWIRLADNEGQVSEIGVLATKILTRENYIVTVPNAVVVSGKIINLSAESADGGVNLTTSVTIGYDTPWRQVHALLELAARRTPGIDQQIAPVVRKLGLLDWYTSYELQVRLLPTTKLPDGRNALHSSIIDVFNEFGVQIMSPNFVMQPKAAVVVPQEAWYAAPAVAPQEPEK
CGGGCGCTGCGCAAAGGCGACTGGATCCGCCTCGCCGATAATGAGGGTCAGGTCAGCGAGATTGGCGTACTGGCGACCAAAATTCTCACCCGCGAAAATTACATCGTTACCGTCCCGAACGCGGTGGTGGTGAGCGGGAAAATTATTAATCTCAGCGCCGAAAGCGCCGACGGTGGAGTCAACCTCACCACCAGCGTCACCATCGGCTACGATACGCCGTGGCGTCAGGTCCATGCGCTGCTGGAGCTGGCGGCGAGACGCACCCCGGGGATCGATCAGCAAATTGCGCCGGTGGTGCGTAAGCTGGGATTGCTTGACTGGTACACCTCGTATGAGCTGCAGGTGCGCTTACTCCCCACAACCAAGCTGCCGGATGGCCGCAACGCATTGCATAGCAGCATTATTGATGTGTTTAACGAATTCGGCGTGCAGATTATGTCGCCGAACTTTGTGATGCAGCCAAAGGCGGCGGTAGTGGTACCGCAGGAAGCCTGGTACGCCGCCCCGGCCGTCGCGCCGCAGGAGCCTGAGAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,219 total letters Query= IDP96922.105 Length=939 Score E Sequences producing significant alignments: (Bits) Value 2_12G_G6_IDP96922.105_T7_Term.seq 1045 0.0 1_06G_G6_IDP96922.105_v73F.seq 966 0.0 0_wt 964 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTG--TACTT-CCAAT-C 194 2 653 ..........A........G....------........--.....-.....-. 611 2 673 .....C........... 657 1 16 .......NGT.....T.....N. 38 S N A R A L R K G D W I R L A D N E G Q V S E I G V L A T K Query_1 195 CAATGCCCGGGCGCT-GCGCAAAGGCGACTGGATCCGCCTCGCCGATAATGAGGGTCAGGTCAGCGAGATTGGCGTACTGGCGACCAAAA 283 2 610 ...............-.......................................................................... 522 1 39 ...............T.......................................................................... 128 0 1 ........-.......................................................................... 82 I L T R E N Y I V T V P N A V V V S G K I I N L S A E S A D Query_1 284 TTCTCACCCGCGAAAATTACATCGTTACCGTCCCGAACGCGGTGGTGGTGAGCGGGAAAATTATTAATCTCAGCGCCGAAAGCGCCGACG 373 2 521 .......................................................................................... 432 1 129 .......................................................................................... 218 0 83 .......................................................................................... 172 G G V N L T T S V T I G Y D T P W R Q V H A L L E L A A R R Query_1 374 GTGGAGTCAACCTCACCACCAGCGTCACCATCGGCTACGATACGCCGTGGCGTCAGGTCCATGCGCTGCTGGAGCTGGCGGCGAGACGCA 463 2 431 .......................................................................................... 342 2 307 ........... 317 1 219 ......................................N..........................................N........ 308 1 343 ........... 333 0 173 .......................................................................................... 262 T P G I D Q Q I A P V V R K L G L L D W Y T S Y E L Q V R L Query_1 464 CCCCGGGGATCGATCAGCAAATTGCGCCGGTGGTGCGTAAGCTGGGATTGCTTGACTGGTACACCTCGTATGAGCTGCAGGTGCGCTTAC 553 2 341 ...........................T.............................................................. 252 1 309 ...........................T.............................................N................ 398 0 263 .......................................................................................... 352 L P T T K L P D G R N A L H S S I I D V F N E F G V Q I M S Query_1 554 TCCCCACAACCAAGCTGCCGGATGGCCGCAACGCATTGCATAGCAGCATTATTGATGTGTTTAACGAATTCGGCGTGCAGATTATGTCGC 643 2 251 .......................................................................................... 162 1 399 ...................N...................................................N.......N.......... 488 0 353 .......................................................................................... 442 P N F V M Q P K A A V V V P Q E A W Y A A P A V A P Q E P E Query_1 644 CGAACTTTGTGATGCAGCCAAAGGCGGCGGTAGTGGTACCGCAGGAAGCCTGGTACGCCGCCCCGGCCGTCGCGCCGCAGGAGCCTGAGA 733 2 161 .....................................................................................C.... 72 1 489 ...................T.............N.............................................T.....C.... 578 0 443 .......................................................................................... 532 K * H W K W I T D P N S S S V D K L A A A L E H H H H H H * Query_1 734 AATAACA-TTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGA 822 2 71 .......T.......N...... 50 1 579 .......T............... 601 0 533 .. 534 D P A A N K A R K E A E L A A A T A E Q * L A * P L G A S Query_1 823 GATCCGGCTGCTAAC-AAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCC-TTGGGGCCTCTA 910 2 47 ..............N...... 27 1 602 ....N.........-....................-.......................-................C............ 687 K R V L R G F L L Query_1 911 AACGGGTCTTGAGGGGTTTTTTGCTGA 937 1 688 ...T....................... 714 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2008776 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:51 PM Number of letters in database: 2,219 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96923.103 (IDP96923.23-427.P(427)..pMCSG73)
ANLSDDGSFQLNGYGMVAGDFQSELNRPKNMSLHTDPTGPNQDPRGKMGDLGNSFWHDYWTSLAITKKWQGLASPEQWADYTYEMVGYGDKTVETAQNYGRFGGLSFLPEGANIWAGRRYLDQRINIFAYNTKEVHIDSGLGYNSKNFDLNVGTDQIDWSSDTAPQAIEGSRRIVDMAYRIDQTELGMTYVKEIDDPLGTGKQKAISFSAKYSMPSFWGVAKGQSTVKLQYGKGVIAPYLNTSRITVLSEEGDCSMRFSTDGNLTLFDDFAVSPAFIYEYTKREKTADRTTFIPDTGYSGGDMTYGSANETGIFTGVNVKQNIHHQNLSMLYEVMVNNTINKNGVEGASGVGYKIAAGPALQLDVLPYVAPILSLTVTYAGGDKEVTLLPEDSEWRVGYRMEVWF
GCCAATCTAAGCGACGACGGCAGCTTCCAGTTAAATGGATATGGCATGGTGGCTGGAGATTTTCAGAGCGAACTAAACCGTCCCAAAAACATGTCGTTGCATACCGATCCCACCGGACCTAATCAGGATCCTCGTGGCAAAATGGGCGATTTGGGAAACAGTTTCTGGCACGATTACTGGACATCGCTGGCTATCACTAAAAAATGGCAGGGGCTGGCTTCTCCCGAGCAATGGGCTGATTACACCTATGAAATGGTGGGTTACGGCGATAAAACGGTAGAAACGGCACAGAATTATGGGCGCTTTGGTGGGCTCTCTTTTCTTCCCGAGGGCGCAAACATTTGGGCTGGGCGTCGTTACCTCGATCAACGTATTAATATCTTCGCTTACAATACCAAAGAAGTACATATTGATTCCGGCCTCGGTTATAACAGTAAGAATTTTGATTTGAACGTTGGTACCGACCAGATCGACTGGTCCAGCGATACCGCGCCGCAGGCGATAGAAGGCTCGCGGCGCATCGTTGATATGGCTTATCGTATCGATCAGACAGAGCTGGGTATGACCTATGTTAAAGAAATTGATGACCCATTGGGCACTGGCAAGCAGAAAGCAATTAGTTTTTCCGCGAAATACAGCATGCCGTCTTTTTGGGGAGTGGCAAAGGGACAATCGACAGTCAAATTACAGTATGGTAAAGGGGTTATTGCTCCTTATCTGAACACCAGTCGTATTACCGTCCTTAGCGAAGAGGGCGATTGTTCAATGCGTTTTTCCACCGACGGAAACTTGACGTTATTTGATGATTTTGCCGTCTCGCCGGCTTTCATCTATGAATATACCAAGCGTGAAAAAACTGCTGACCGAACCACCTTTATACCTGATACAGGTTATTCAGGGGGGGACATGACCTATGGTTCCGCAAATGAAACCGGGATCTTTACCGGCGTAAACGTTAAACAAAATATTCATCACCAAAATCTCTCCATGTTGTATGAAGTCATGGTGAATAACACTATCAATAAAAATGGCGTCGAAGGTGCCAGCGGCGTCGGCTACAAAATTGCCGCCGGCCCGGCCCTGCAACTGGATGTTTTACCGTATGTCGCGCCGATTCTCAGTTTGACGGTTACCTACGCAGGCGGGGACAAAGAGGTCACGTTATTGCCGGAAGACTCGGAGTGGCGAGTCGGGTATCGAATGGAAGTCTGGTTC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,681 total letters Query= IDP96923.103 Length=1620 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2192 0.0 1_06H_H6_IDP96923.103_v73F.seq 1864 0.0 2_12H_H6_IDP96923.103_T7_Term.seq 1860 0.0 F V * L * E G D I H M H H H H H H S S G V D L W S H P Q F Query_1 74 ATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCG 163 0 966 ............G.. 952 1 44 ...........A....... 62 1 26 .....C........... 42 1 1058 ............G... 1043 2 314 ............G.. 328 E K G T E N L Y F Q S N A A N L S D D G S F Q L N G Y G M V Query_1 164 AAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGCCAATCTAAGCGACGACGGCAGCTTCCAGTTAAATGGATATGGCATGGTGG 253 0 1 .................................................... 52 1 63 .G....------.............................................................................. 146 A G D F Q S E L N R P K N M S L H T D P T G P N Q D P R G K Query_1 254 CTGGAGATTTTCAGAGCGAACTAAACCGTCCCAAAAACATGTCGTTGCATACCGATCCCACCGGACCTAATCAGGATCCTCGTGGCAAAA 343 0 53 .......................................................................................... 142 1 147 .......................................................................................... 236 2 1158 ......-......N.T........... 1133 M G D L G N S F W H D Y W T S L A I T K K W Q G L A S P E Q Query_1 344 TGGGCGATTTGGGAAACAGTTTCTGGCACGATTACTGGACATCGCTGGCTATCACTAAAAAATGGCAGGGGCTGGCTTCTCCCGAGCAAT 433 0 143 .......................................................................................... 232 1 237 ..............................................................................T........... 326 2 1132 ...-........A.NCA.......NN-.....-....ACA........................N.............TN.--....... 1048 W A D Y T Y E M V G Y G D K T V E T A Q N Y G R F G G L S F Query_1 434 GGGCTGATTACACCTATGAAATGGTGGGTTACGGCGATAAAACGGTAGAAACGGCACAGAATTATGGGCGCTTTGGTGGGCTCTCTTTTC 523 0 233 .......................................................................................... 322 1 327 .......................................................................................... 416 2 1047 ........-..N.-....................................-...........A........................... 961 L P E G A N I W A G R R Y L D Q R I N I F A Y N T K E V H Query_1 524 TTCCCGAGGG-CGCAAACATTTGGGCTGGGCGTCGTTACCTCGATCAACGTATTAATATCTTCGCTTACAATACCAAAG-AAGTACATAT 611 0 323 ..........-....................................................................-.......... 410 1 417 ..........-....................................................................-.......... 504 2 960 ..........G....................................................................N.......... 871 I D S G L G Y N S K N F D L N V G T D Q I D W S S D T A P Q Query_1 612 TGATTCCGG-CCTCGGTTATAACAGTAAGAATTTTGATTTGAACGTTGGTACCGACCAGATCGACTGGTCCAGCGATACCGCGCCGCAGG 700 0 411 .........-................................................................................ 499 1 505 .........-................................................................................ 593 2 870 .........G................................................................................ 781 A I E G S R R I V D M A Y R I D Q T E L G M T Y V K E I D D Query_1 701 CGATAGAAGGCTCGCGGCGCATCGTTGATATGGCTTATCGTATCGATCAGACAGAGCTGGGTATGACCTATGTTAAAGAAATTGATGACC 790 0 500 .......................................................................................... 589 1 594 .......................................................................................... 683 2 780 .......................................................................................... 691 P L G T G K Q K A I S F S A K Y S M P S F W G V A K G Q S T Query_1 791 CATTGGGCACTGGCAAGCAGAAAGCAATTAGTTTTTCCGCGAAATACAGCATGCCGTCTTTTTGGGGAGTGGCAAAGGGACAATCGACAG 880 0 590 .......................................................................................... 679 1 684 .......................................................................................... 773 2 690 .......................................................................................... 601 V K L Q Y G K G V I A P Y L N T S R I T V L S E E G D C S M Query_1 881 TCAAATTACAGTATGGTAAAGGGGTTATTGCTCCTTATCTGAACACCAGTCGTATTACCGTCCTTAGCGAAGAGGGCGATTGTTCAATGC 970 0 680 .......................................................................................... 769 1 774 .......................................................................................... 863 2 600 .......................................................................................... 511 R F S T D G N L T L F D D F A V S P A F I Y E Y T K R E K T Query_1 971 GTTTTTCCACCGACGGAAACTTGACGTTATTTGATGATTTTGCCGTCTCGCCGGCTTTCATCTATGAATATACCAAGCGTGAAAAAACTG 1060 0 770 .......................................................................................... 859 1 864 .......................................................................................... 953 2 510 .......................................................................................... 421 A D R T T F I P D T G Y S G G D M T Y G S A N E T G I F T G Query_1 1061 CTGACCGAACCACCTTTATACCTGATACAGGTTATTCAgggggggACATGACCTATGGTTCCGCAAATGAAACCGGGATCTTTACCGGCG 1150 0 860 .......................................................................................... 949 1 954 .............................-............................N....................N.......... 1042 2 420 .......................................................................................... 331 V N V K Q N I H H Q N L S M L Y E V M V N N T I N K N G V E Query_1 1151 TAAACGTTAAACAAAATATTCATCACCAAAATCTCTCCATGTTGTATGAAGTCATGGTGAATAACACTATCAATAAAAATGGCGTCGAAG 1240 0 950 .......................................................................................... 1039 1 1043 .-........................--N.......-...-NN.N............N....-.N...............-......... 1125 2 330 .......................................................................................... 241 G A S G V G Y K I A A G P A L Q L D V L P Y V A P I L S L T Query_1 1241 GTGCCAGCGGCGTCGGCTACAAAATTGCCGCCGGCCCGGCCCTGCAACTGGATGTTTTACCGTATGTCGCGCCGATTCTCAGTTTGACGG 1330 0 1040 .......................................................................................... 1129 1 1126 ......... 1134 2 240 .......................................................................................... 151 V T Y A G G D K E V T L L P E D S E W R V G Y R M E V W F * Query_1 1331 TTACCTACGCAGGCGGGGACAAAGAGGTCACGTTATTGCCGGAAGACTCGGAGTGGCGAGTCGGGTATCGAATGGAAGTCTGGTTCTAAC 1420 0 1130 ...................................................................................... 1215 2 150 ...........................................................................-.............. 62 H W K W I T D P N S S S V D K L A A A L E H H H H H H * D P Query_1 1421 ATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGG 1510 2 61 ................--..... 41 2 45 ...... 40 A A N K A R K E Query_1 1511 CTGCTAACAAAGCCCGAAAGGAA 1533 2 39 ...................A... 17 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5827332 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:51 PM Number of letters in database: 3,681 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96925.103 (IDP96925.1-239.F..pMCSG73)
MKMIVTEDYEEMSLVASHHVLGYITAPRRVNLAVTAGSTPKRMYEHLTAAVKGKAFYDRVHYYNFDEIPFRGQSREGVTISNLRQLFFTPAQIKEENIHKLTLDNAAQHDRQLEEAGGLDLMVLGLGADGHFCGNLPNTTRFHDQTVEVPIHGEMIALIANSEMGGDISAVPNSYVTMGPRSVMAAKNLLLIVSGAAKAHALKQVVEGPVSVQVPASVLKLHPSLVIIADKAAAAELQQ
ATGAAAATGATTGTGACCGAAGATTATGAAGAGATGAGCCTGGTCGCCAGCCATCATGTTCTGGGCTATATCACGGCGCCGCGGCGGGTGAATCTGGCGGTGACGGCGGGGAGCACGCCAAAGCGGATGTATGAGCATTTGACCGCCGCCGTGAAAGGCAAAGCATTCTATGATCGGGTGCACTATTACAACTTCGATGAGATCCCTTTTCGCGGCCAGTCGCGAGAAGGCGTCACGATATCTAATTTGCGCCAGCTGTTTTTTACCCCGGCGCAGATCAAAGAAGAGAACATTCACAAGCTGACGCTGGACAACGCCGCTCAGCACGATCGCCAGCTGGAAGAGGCTGGCGGTCTGGATCTGATGGTGCTGGGGCTGGGGGCCGACGGCCATTTTTGCGGCAACCTGCCGAATACCACCCGCTTTCATGATCAGACGGTCGAAGTGCCGATTCATGGGGAAATGATTGCTCTTATCGCTAACAGCGAGATGGGCGGGGATATCTCGGCAGTACCAAACAGCTATGTCACCATGGGGCCGAGAAGCGTGATGGCGGCGAAAAATTTATTGCTAATTGTCAGCGGTGCGGCAAAAGCGCACGCGTTAAAGCAAGTGGTGGAAGGTCCGGTCAGCGTGCAGGTGCCGGCTTCAGTGCTCAAATTGCACCCTTCACTGGTGATTATTGCCGATAAAGCGGCGGCGGCGGAGTTGCAGCAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,201 total letters Query= IDP96925.103 Length=1122 Score E Sequences producing significant alignments: (Bits) Value 2_03A_A7_IDP96925.103_v73F.seq 1393 0.0 1_01A_A7_IDP96925.103_T7Term.seq 1391 0.0 0_wt 1294 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 2 46 ........A........G....------....................... 90 2 27 .............. 40 1 834 ...........A........G....------....................... 787 1 852 .....C........... 836 A M K M I V T E D Y E E M S L V A S H H V L G Y I T A P R R Query_1 199 GCCATGAAAATGATTGTGACCGAAGATTATGAAGAGATGAGCCTGGTCGCCAGCCATCATGTTCTGGGCTATATCACGGCGCCGCGGCGG 288 2 91 .......................................................................................... 180 2 1002 ........ 1009 1 786 .......................................................................................... 697 0 1 ....................................................................................... 87 V N L A V T A G S T P K R M Y E H L T A A V K G K A F Y D Query_1 289 GTGAATCTGGCGGTGACGGCGGGGAGCACGCCAAAGCGGATGTATGAGCATTT-GACCGCCGCCGTGAAAGGCAAAGCATTCTATGATCG 377 2 181 .....................................................-.................................... 269 2 1010 ... 1012 1 696 .....................................................T.................................... 607 0 88 .....................................................-.................................... 176 R V H Y Y N F D E I P F R G Q S R E G V T I S N L R Q L F F Query_1 378 GGTGCACTATTACAACTTCGATGAGATCCCTTTTCGCGGCCAGTCGCGAGAAGGCGTCACGATATCTAATTTGCGCCAGCTGTTTTTTAC 467 2 270 .......................................................................................... 359 1 606 .......................................................................................... 517 0 177 .......................................................................................... 266 T P A Q I K E E N I H K L T L D N A A Q H D R Q L E E A G G Query_1 468 CCCGGCGCAGATCAAAGAAGAGAACATTCACAAGCTGACGCTGGACAACGCCGCTCAGCACGATCGCCAGCTGGAAGAGGCTGGCGGTCT 557 2 360 .......................................................................................... 449 1 516 .......................................................................................... 427 0 267 .......................................................................................... 356 L D L M V L G L G A D G H F C G N L P N T T R F H D Q T V E Query_1 558 GGATCTGATGGTGCTGGGGCTGGGGGCCGACGGCCATTTTTGCGGCAACCTGCCGAATACCACCCGCTTTCATGATCAGACGGTCGAAGT 647 2 450 .......................................................................................... 539 1 426 .......................................................................................... 337 0 357 .......................................................................................... 446 V P I H G E M I A L I A N S E M G G D I S A V P N S Y V T M Query_1 648 GCCGATTCATGGGGAAATGATTGCTCTTATCGCTAACAGCGAGATGGGCGGGGATATCTCGGCAGTACCAAACAGCTATGTCACCATGGG 737 2 540 .......................................................................................... 629 1 336 .......................................................................................... 247 0 447 .......................................................................................... 536 G P R S V M A A K N L L L I V S G A A K A H A L K Q V V E G Query_1 738 GCCGAGAAGCGTGATGGCGGCGAAAAATTTATTGCTAATTGTCAGCGGTGCGGCAAAAGCGCACGCGTTAAAGCAAGTGGTGGAAGGTCC 827 2 630 .......................................................................................... 719 1 246 .......................................................................................... 157 0 537 .......................................................................................... 626 P V S V Q V P A S V L K L H P S L V I I A D K A A A A E L Q Query_1 828 GGTCAGCGTGCAGGTGCCGGCTTCAGTGCTCAAATTGCACCCTTCACTGGTGATTATTGCCGATAAAGCGGCGGCGGCGGAGTTGCAGCA 917 2 720 .......................................................................................... 809 1 156 .......................................................................................... 67 0 627 .......................................................................................... 716 Q * H W K W I T D P N S S S V D K L A A A L E H H H H H H * Query_1 918 ATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGA 1007 2 810 .....................--..... 835 2 831 . 831 1 66 .....................--..... 41 1 45 . 45 0 717 . 717 D P A A N K A R K E A E L A A A T A E Q * L A * P L G A S K Query_1 1008 TCCGGCTGCTAACAAAGCCCG-AAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAAC 1096 2 832 .....................-.................................................................... 920 1 44 .....................A....... 16 R V L R G F L L K Query_1 1097 GGGTCTTGAGGGGTTTTTTGCTGAAA 1122 2 921 -........-................ 944 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3487218 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:51 PM Number of letters in database: 3,201 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96926.103 (IDP96926.1-335.F..pMCSG73)
MIYVIAVGDEKWQAWPPLLRAVYQIMQLQKLVNMFGGDLMRRYGEKVHKLTLHGGFSCPNRDGTLGRGGCTFCNVASFADEAQQHRSIAEQLAHQAQLVNRAKRYLAYFQAYTSTFAEVQVLRSMYRQAVSAANIVGLCVGTRPDCVPQAVLDLLSEYHQQGYEVWLELGLQTAHDKTLHRINRGHDFACYQRTARLARERGLKVCAHLIVGLPGESQGHCLQTLEQVVATGVDGIKLHPLHIVKGSIMAKAWQAGRLDGIELEAYTVTAGEMIRHTPPEVIYHRISASARRPTLLAPLWCENRWTGMVELDRYLNQQGAQGSALGRSWVAPQAE
TTGATCTATGTCATCGCCGTCGGTGACGAAAAGTGGCAGGCTTGGCCCCCTTTGTTGAGGGCGGTCTATCAGATTATGCAGTTACAGAAATTAGTCAATATGTTTGGTGGGGATCTTATGCGTCGCTATGGCGAAAAGGTCCATAAGCTAACGCTGCACGGCGGTTTTAGCTGCCCAAACCGCGACGGCACGCTGGGCCGCGGCGGTTGCACTTTCTGTAACGTGGCATCGTTTGCCGATGAGGCGCAGCAGCATCGCTCTATCGCCGAACAGCTCGCTCATCAGGCGCAGCTCGTCAACCGGGCGAAGCGCTATCTGGCCTATTTTCAGGCCTACACCAGCACCTTCGCTGAAGTGCAGGTGCTCCGTTCAATGTATCGTCAGGCCGTCAGTGCCGCAAATATCGTTGGCCTGTGCGTCGGCACCCGCCCGGACTGCGTTCCGCAGGCGGTCCTTGACCTGCTGAGTGAATACCATCAGCAAGGGTATGAGGTATGGCTGGAGCTGGGGCTGCAAACTGCTCATGACAAGACCCTGCATCGCATTAACCGTGGACACGATTTCGCCTGTTATCAGCGTACCGCGCGCCTGGCGCGAGAGCGGGGGCTGAAAGTCTGTGCGCATCTGATCGTCGGTCTGCCCGGAGAGAGCCAGGGGCATTGTCTGCAGACGCTGGAGCAGGTAGTGGCGACCGGCGTGGATGGGATCAAATTGCATCCACTGCATATTGTGAAAGGCAGCATTATGGCCAAAGCGTGGCAGGCCGGGCGTCTCGATGGCATTGAGCTGGAGGCGTATACCGTCACCGCTGGAGAGATGATCCGCCATACGCCGCCGGAGGTGATCTACCACCGTATTTCCGCCAGCGCCCGTCGGCCCACGCTGTTGGCGCCGCTGTGGTGTGAAAATCGCTGGACCGGCATGGTTGAACTGGATCGTTATCTGAATCAGCAGGGCGCTCAGGGCTCGGCGCTGGGCCGTAGCTGGGTTGCTCCCCAGGCTGAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,460 total letters Query= IDP96926.103 Length=1410 Score E Sequences producing significant alignments: (Bits) Value 2_03B_B7_IDP96926.103_v73F.seq 1842 0.0 1_01B_B7_IDP96926.103_T7Term.seq 1837 0.0 0_wt 1813 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 2 42 ...........A........G....------....................... 89 2 24 .....C........... 40 1 1088 ..N....N..... 1076 A L I Y V I A V G D E K W Q A W P P L L R A V Y Q I M Q L Query_1 199 GCCTTGATCTATGTCAT-CGCCGTCGGTGACGAAAAGTGGCAGGCTTGGCCCCCTTT-GTTGAGGGCGGTCTATCAGATTATGCAGTTAC 286 2 90 ...A.............-.......................................-................................ 177 1 1075 ...A.............T.................-.........N...........T................................ 987 0 1 ..............-.......................................-................................ 85 Q K L V N M F G G D L M R R Y G E K V H K L T L H G G F S C Query_1 287 AGAAATTAGTCAATATGTTTGGTGGGG-ATCTTATGCGTCGCTATGGCGAAAAGGTCCATAAGCTAACGCTGCACGGCGGTTTTAGCTGC 375 2 178 ...........................-.............................................................. 266 1 986 ...........................G.............................................................. 897 0 86 ...........................-.............................................................. 174 P N R D G T L G R G G C T F C N V A S F A D E A Q Q H R S I Query_1 376 CCAAACCGCGACGGCACGCTGGGCCGCGGCGGTTGCACTTTCTGTAACGTGGCATCGTTTGCCGATGAGGCGCAGCAGCATCGCTCTATC 465 2 267 .......................................................................................... 356 1 896 .......................................................................................... 807 0 175 .......................................................................................... 264 A E Q L A H Q A Q L V N R A K R Y L A Y F Q A Y T S T F A E Query_1 466 GCCGAACAGCTCGCTCATCAGGCGCAGCTCGTCAACCGGGCGAAGCGCTATCTGGCCTATTTTCAGGCCTACACCAGCACCTTCGCTGAA 555 2 357 .......................................................................................... 446 1 806 .......................................................................................... 717 0 265 .......................................................................................... 354 V Q V L R S M Y R Q A V S A A N I V G L C V G T R P D C V P Query_1 556 GTGCAGGTGCTCCGTTCAATGTATCGTCAGGCCGTCAGTGCCGCAAATATCGTTGGCCTGTGCGTCGGCACCCGCCCGGACTGCGTTCCG 645 2 447 .......................................................................................... 536 1 716 .......................................................................................... 627 0 355 .......................................................................................... 444 Q A V L D L L S E Y H Q Q G Y E V W L E L G L Q T A H D K T Query_1 646 CAGGCGGTCCTTGACCTGCTGAGTGAATACCATCAGCAAGGGTATGAGGTATGGCTGGAGCTGGGGCTGCAAACTGCTCATGACAAGACC 735 2 537 .......................................................................................... 626 1 626 .......................................................................................... 537 0 445 .......................................................................................... 534 L H R I N R G H D F A C Y Q R T A R L A R E R G L K V C A H Query_1 736 CTGCATCGCATTAACCGTGGACACGATTTCGCCTGTTATCAGCGTACCGCGCGCCTGGCGCGAGAGCGGGGGCTGAAAGTCTGTGCGCAT 825 2 627 .......................................................................................... 716 1 536 .......................................................................................... 447 0 535 .......................................................................................... 624 L I V G L P G E S Q G H C L Q T L E Q V V A T G V D G I K L Query_1 826 CTGATCGTCGGTCTGCCCGGAGAGAGCCAGGGGCATTGTCTGCAGACGCTGGAGCAGGTAGTGGCGACCGGCGTGGATGGGATCAAATTG 915 2 717 .......................................................................................... 806 1 446 .......................................................................................... 357 0 625 .......................................................................................... 714 H P L H I V K G S I M A K A W Q A G R L D G I E L E A Y T V Query_1 916 CATCCACTGCATATTGTGAAAGGCAGCATTATGGCCAAAGCGTGGCAGGCCGGGCGTCTCGATGGCATTGAGCTGGAGGCGTATACCGTC 1005 2 807 .......................................................................................... 896 1 356 .......................................................................................... 267 0 715 .......................................................................................... 804 T A G E M I R H T P P E V I Y H R I S A S A R R P T L L A P Query_1 1006 ACCGCTGGAGAGATGATCCGCCATACGCCGCCGGAGGTGATCTACCACCGTATTTCCGCCAGCGCCCGTCGGCCCACGCTGTTGGCGCCG 1095 2 897 .......................................................................................... 986 1 266 .......................................................................................... 177 0 805 .......................................................................................... 894 L W C E N R W T G M V E L D R Y L N Q Q G A Q G S A L G R S Query_1 1096 CTGTGGTGTGAAAATCGCTGGACCGGCATGGTTGAACTGGATCGTTATCTGAATCAGCAGGGCGCTCAGGGCTCGGCGCTGGGCCGTAGC 1185 2 987 ..........-....................N....N...N..................-..N.............N......NN..... 1074 1 176 .......................................................................................... 87 0 895 .......................................................................................... 984 W V A P Q A E * H W K W I T D P N S S S V D K L A A A L E Query_1 1186 TGGGTTG-CTCCCCAGGCTGAGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGca 1274 2 1075 .......C...................... 1104 1 86 .......-..................................--..... 41 0 985 .......-.............. 1005 H H H H H H * D P A A N K A R K E A E L A A A T A E Q * L Query_1 1275 ccaccaccaccaccacTGAGATCC-GGCTGCTAACAAAGCCC--GAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGC 1361 2 1117 ....N............NT..G----.NNNN.....NNN--.............N..NN..--N.N.... 1178 1 45 ....-.................CGA........ 14 A * P L G A S K R V L R G F L L K Query_1 1362 ATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAA 1410 2 1179 NNNN..---NN..N....NN..-N..NN.......N.....GC...... 1223 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4756328 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:51 PM Number of letters in database: 3,460 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96927.102 (IDP96927.1-460.F..pMCSG68)
MKIMSASLDDVKDFDFVISEAFRIDVGLDSPNFREFYNQEYNRDFGSIGMTINAPIELISKNRTIPQRQIHLSYASNERLHLISARFIKSNRLEMLVTVDEFVENIKKSLYVHTFIDDGDDGIKGYTQLLNKALKKTKREMIQEDFFFPILAHGLDKEKICIGRAEIISAQHMYSMINDNLTEEQILLSRRFCSAHKYHYGHFLRIPVSKRSKKSRERIAKNIAGFVVGILQLFSEHYHISPDFLSLSTSPYPNYEGFYFTRTSNNDFNYNHSSKGRITWSDKFWDKFESDYNSDLGEVLSRLIDLAIEPNERTIIADRLIDAIYLFSSAQQDKDDSSRIVKLATALERLVSLPSEKKDESTTKNFVSRVSSLVSIYYPDEKDWAKVSKEMYEIRSNITHGAWSLYRGVDPLYSSRYSELTSRAIFSACIGFYKRSFTSDNNDRLVKEFFDFLENAIKEK
GTGAAAATAATGAGTGCAAGTTTAGATGATGTAAAAGATTTTGACTTTGTGATCTCAGAAGCATTTAGAATAGATGTTGGATTAGATTCACCTAATTTTAGAGAGTTTTATAATCAAGAGTACAATAGAGATTTTGGTTCAATCGGAATGACAATAAATGCACCGATTGAGTTAATCTCAAAGAATAGAACAATTCCACAAAGGCAAATACATCTTTCATACGCTTCAAATGAGAGATTGCATTTAATATCCGCAAGGTTCATAAAATCAAATAGGCTAGAAATGTTAGTCACAGTTGATGAGTTTGTTGAAAATATAAAGAAAAGTCTTTATGTTCATACTTTCATTGATGACGGTGACGATGGCATAAAAGGATATACCCAACTTCTCAATAAAGCACTAAAAAAAACAAAGAGAGAAATGATACAAGAAGATTTCTTTTTCCCTATCTTGGCCCATGGCCTTGATAAAGAGAAGATATGCATTGGTCGCGCTGAGATTATATCAGCACAGCATATGTACTCAATGATTAACGATAACCTTACAGAAGAACAAATATTATTATCCAGAAGATTTTGCTCTGCACATAAATATCACTACGGACATTTCTTAAGAATTCCAGTTAGCAAGCGCTCTAAGAAAAGCCGAGAGAGAATAGCTAAAAACATTGCAGGGTTTGTAGTTGGAATACTCCAATTATTCAGTGAACATTATCACATATCACCTGATTTCTTGAGCCTTTCAACGAGTCCATATCCTAATTATGAAGGTTTTTACTTCACAAGAACATCAAATAATGATTTCAACTATAATCACTCATCCAAGGGAAGAATAACTTGGAGCGACAAATTCTGGGATAAATTTGAAAGTGATTATAATTCAGACTTAGGGGAGGTACTGTCTCGTCTAATAGATCTAGCCATAGAACCCAATGAGAGAACAATAATTGCTGATCGATTGATTGATGCCATTTATTTGTTTAGCAGCGCTCAACAGGATAAAGATGATAGTTCACGCATAGTAAAATTAGCAACAGCCCTAGAACGATTGGTAAGCCTTCCAAGCGAGAAAAAGGATGAGTCTACAACTAAAAACTTCGTAAGCCGAGTATCGTCATTGGTTTCCATTTATTATCCTGATGAAAAAGACTGGGCTAAGGTTTCAAAAGAAATGTATGAAATCAGATCTAATATTACTCACGGCGCATGGTCTTTATATCGTGGGGTAGATCCATTATATTCATCTAGATATAGCGAATTAACAAGCAGAGCCATTTTCTCTGCGTGCATAGGGTTTTATAAACGAAGTTTTACTAGCGATAACAATGATCGATTAGTAAAAGAATTCTTCGATTTTTTAGAAAATGCCATAAAAGAAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,830 total letters Query= IDP96927.102 Length=1785 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2489 0.0 2_08H_H2_IDP96927.102_T7term.seq 1952 0.0 1_02H_H2_IDP96927.102_T7S.seq 1918 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 58 TTCCCCT-CTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTG 146 1 6 .......T......-........................................................................... 94 W S H P Q F E K G T E N L Y F Q S N A V K I M S A S L D D V Query_1 147 GTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGTGAAAATAATGAGTGCAAGTTTAGATGATGTAAA 236 0 1 ................................... 35 1 95 .......................................................A.................................. 184 K D F D F V I S E A F R I D V G L D S P N F R E F Y N Q E Y Query_1 237 AGATTTTGACTTTGTGATCTCAGAAGCATTTAGAATAGATGTTGGATTAGATTCACCTAATTTTAGAGAGTTTTATAATCAAGAGTACAA 326 0 36 .......................................................................................... 125 1 185 .......................................................................................... 274 N R D F G S I G M T I N A P I E L I S K N R T I P Q R Q I H Query_1 327 TAGAGATTTTGGTTCAATCGGAATGACAATAAATGCACCGATTGAGTTAATCTCAAAGAATAGAACAATTCCACAAAGGCAAATACATCT 416 0 126 .......................................................................................... 215 1 275 .......................................................................................... 364 L S Y A S N E R L H L I S A R F I K S N R L E M L V T V D E Query_1 417 TTCATACGCTTCAAATGAGAGATTGCATTTAATATCCGCAAGGTTCATAAAATCAAATAGGCTAGAAATGTTAGTCACAGTTGATGAGTT 506 0 216 .......................................................................................... 305 1 365 .......................................................................................... 454 F V E N I K K S L Y V H T F I D D G D D G I K G Y T Q L L N Query_1 507 TGTTGAAAATATAAAGAAAAGTCTTTATGTTCATACTTTCATTGATGACGGTGACGATGGCATAAAAGGATATACCCAACTTCTCAATAA 596 0 306 .......................................................................................... 395 2 1170 ......N.NNCA.................-.............N..NNNNN..---.... 1115 1 455 .......................................................................................... 544 K A L K K T K R E M I Q E D F F F P I L A H G L D K E K I C Query_1 597 AGCACTaaaaaaaaCAAAGAGA-GAAATGATACAAGAAGATTTCTTTTTCCCTATCTTGGCCCATGGCCTTGATAAAGAGAAGATATGCA 685 0 396 ......................-................................................................... 484 2 1114 ..............N..-...NN...........-..N.............-..N..NN-......-....................... 1030 1 545 ......................-................................................................... 633 I G R A E I I S A Q H M Y S M I N D N L T E E Q I L L S R R Query_1 686 TTGGTCGCGCTGAGATTATATCAGCACAGCATATGTACTCAATGATTAACGATAACCTTACAGAAGAACAAATATTATTATCCAGAAGAT 775 0 485 .......................................................................................... 574 2 1029 .N...............N........................................................................ 940 1 634 .......................................................................................... 723 F C S A H K Y H Y G H F L R I P V S K R S K K S R E R I A K Query_1 776 TTTGCTCTGCACATAAATATCACTACGGACATTTCTTAAGAATTCCAGTTAGCAAGCGCTCTAAGAAAAGCCGAGAGAGAATAGCTAAAA 865 0 575 .......................................................................................... 664 2 939 .......................................................................................... 850 1 724 .......................................................................................... 813 N I A G F V V G I L Q L F S E H Y H I S P D F L S L S T S P Query_1 866 ACATTGCAGGGTTTGTAGTTGGAATACTCCAATTATTCAGTGAACATTATCACATATCACCTGATTTCTTGAGCCTTTCAACGAGTCCAT 955 0 665 .......................................................................................... 754 2 849 .......................................................................................... 760 1 814 .......................................................................................... 903 Y P N Y E G F Y F T R T S N N D F N Y N H S S K G R I T W S Query_1 956 ATCCTAATTATGAAGGTTTTTACTTCACAAGAACATCAAATAATGATTTCAACTATAATCACTCATCCAAGGGAAGAATAACTTGGAGCG 1045 0 755 .......................................................................................... 844 2 759 .......................................................................................... 670 1 904 ............................-...........................-......................-......N... 990 D K F W D K F E S D Y N S D L G E V L S R L I D L A I E P N Query_1 1046 ACAAATTCTGGGATAAATTTGAAAGTGATTATAATTCAGACTTAGGGGAGGTACTGTCTCGTCTAATAGATCTAGCCATAGAACCCAATG 1135 0 845 .......................................................................................... 934 2 669 .......................................................................................... 580 1 991 ..-..............-..............-.N.............................-.N..........NN......A.TG. 1076 E R T I I A D R L I D A I Y L F S S A Q Q D K D D S S R I V Query_1 1136 AGAGAACAATAATTGCTGATCGATTGATTGATGCCATTTATTTGTTTAGCAGCGCTCAACAGGATAAAGATGATAGTTCACGCATAGTAA 1225 0 935 .......................................................................................... 1024 2 579 .......................................................................................... 490 1 1077 .......N....N....................G.NN..TA..NN............-.........N... 1146 K L A T A L E R L V S L P S E K K D E S T T K N F V S R V S Query_1 1226 AATTAGCAACAGCCCTAGAACGATTGGTAAGCCTTCCAAGCGAGAAAAAGGATGAGTCTACAACTAAAAACTTCGTAAGCCGAGTATCGT 1315 0 1025 .......................................................................................... 1114 2 489 .......................................................................................... 400 S L V S I Y Y P D E K D W A K V S K E M Y E I R S N I T H G Query_1 1316 CATTGGTTTCCATTTATTATCCTGATGAAAAAGACTGGGCTAAGGTTTCAAAAGAAATGTATGAAATCAGATCTAATATTACTCACGGCG 1405 0 1115 .......................................................................................... 1204 2 399 .......................................................................................... 310 A W S L Y R G V D P L Y S S R Y S E L T S R A I F S A C I G Query_1 1406 CATGGTCTTTATATCGTGGGGTAGATCCATTATATTCATCTAGATATAGCGAATTAACAAGCAGAGCCATTTTCTCTGCGTGCATAGGGT 1495 0 1205 .......................................................................................... 1294 2 309 .......................................................................................... 220 F Y K R S F T S D N N D R L V K E F F D F L E N A I K E K * Query_1 1496 TTTATAAACGAAGTTTTACTAGCGATAACAATGATCGATTAGTAAAAGAATTCTTCGATTTTTTAGAAAATGCCATAAAAGAAAAATAAC 1585 0 1295 ...................................................................................... 1380 2 219 .......................................................................................... 130 H W K W I T D P N S S S V D K L A A A L E H H H H H H * D P Query_1 1586 ATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGG 1675 2 129 .......................................................................................... 40 A A N K A R K Query_1 1676 CTGCTAACAAAGCCCGAAAGG 1696 2 39 ..................... 19 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 6690358 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:51 PM Number of letters in database: 3,830 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96930.102 (IDP96930.24-382.P(382)..pMCSG68)
GKTSILLVTDKNVGALEATQAIHRLLAAEGREVEVIDSVPAEPNHHDVTQIVSQLGASQPQMVVGIGGGSVLDVAKLLSVLLHPEAPSLTSLLAGEQPQRRICSLLIPATAGTGSEATPNAILAIPEQQTKVGIISPVLLPDYVALLPELTTSMPPSIAASTGIDALCHLLECFTSTVANPVSDNAALIGLHKLVRHIERSVNQPQDLTAKLEMLWASWYGGAAINYSGTHLVHALSYPLGGTWHLPHGVANAILLAPCMRVVRPHAVAKFAQVWDLIPDADRTLSAEEKSHALVAWLAALVKRLPLPDNLAALGVPQESISALSAAAQNVKRLMNNAPCSVSREEIAAIYQTLYPEHA
GGTAAAACCTCAATCCTGCTGGTGACCGACAAGAACGTCGGGGCGCTGGAGGCAACCCAGGCGATTCATCGCCTGCTGGCGGCTGAAGGGCGTGAAGTTGAGGTCATTGACAGCGTGCCAGCGGAGCCCAACCATCACGATGTTACGCAGATCGTCAGCCAGCTGGGCGCCAGCCAGCCGCAGATGGTCGTTGGTATCGGCGGGGGGAGCGTACTGGATGTAGCAAAACTGCTGTCAGTACTTCTGCACCCTGAGGCGCCCTCGCTGACGTCCCTGCTGGCAGGCGAGCAGCCGCAACGACGAATTTGCTCATTGTTAATCCCGGCCACCGCGGGCACCGGATCCGAAGCGACGCCGAACGCCATTCTGGCGATCCCCGAGCAGCAGACGAAGGTGGGGATCATCTCCCCGGTGCTGCTTCCGGACTATGTCGCCCTGCTGCCGGAGCTGACCACCAGCATGCCGCCCAGCATCGCGGCGTCGACCGGAATCGATGCTCTGTGTCATCTGCTGGAGTGCTTTACCTCCACCGTCGCCAACCCGGTGAGCGATAACGCGGCGTTAATCGGTTTACACAAGCTGGTACGCCATATCGAGCGTTCGGTAAATCAGCCGCAGGATCTGACGGCGAAGCTGGAGATGCTCTGGGCCTCGTGGTACGGCGGCGCAGCGATCAACTATTCCGGCACTCACCTGGTGCATGCGCTCTCCTATCCGCTTGGCGGTACCTGGCACCTGCCGCACGGGGTGGCCAACGCCATTCTACTGGCGCCCTGCATGCGGGTGGTCCGCCCTCACGCGGTGGCGAAGTTCGCTCAAGTCTGGGACCTGATCCCCGATGCAGATCGCACGCTCAGCGCGGAAGAGAAATCCCATGCGCTGGTCGCATGGCTGGCGGCGCTGGTCAAACGACTGCCGCTGCCGGACAACCTGGCCGCGCTAGGCGTGCCTCAGGAGAGTATTTCCGCCCTCAGCGCAGCGGCGCAAAACGTCAAACGCCTGATGAACAATGCCCCATGCAGCGTCAGCCGCGAAGAGATCGCGGCCATCTACCAAACGCTGTATCCAGAACATGCC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 1,087 total letters Query= IDP96930.102 Length=1482 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1943 0.0 G K T S I L L V T D K N V G A L E A T Q A I H R L L A A E G Query_1 202 GGTAAAACCTCAATCCTGCTGGTGACCGACAAGAACGTCGGGGCGCTGGAGGCAACCCAGGCGATTCATCGCCTGCTGGCGGCTGAAGGG 291 0 1 .......................................................................................... 90 R E V E V I D S V P A E P N H H D V T Q I V S Q L G A S Q P Query_1 292 CGTGAAGTTGAGGTCATTGACAGCGTGCCAGCGGAGCCCAACCATCACGATGTTACGCAGATCGTCAGCCAGCTGGGCGCCAGCCAGCCG 381 0 91 .......................................................................................... 180 Q M V V G I G G G S V L D V A K L L S V L L H P E A P S L T Query_1 382 CAGATGGTCGTTGGTATCGGCGGGGGGAGCGTACTGGATGTAGCAAAACTGCTGTCAGTACTTCTGCACCCTGAGGCGCCCTCGCTGACG 471 0 181 .......................................................................................... 270 S L L A G E Q P Q R R I C S L L I P A T A G T G S E A T P N Query_1 472 TCCCTGCTGGCAGGCGAGCAGCCGCAACGACGAATTTGCTCATTGTTAATCCCGGCCACCGCGGGCACCGGATCCGAAGCGACGCCGAAC 561 0 271 .......................................................................................... 360 0 754 ... 756 A I L A I P E Q Q T K V G I I S P V L L P D Y V A L L P E L Query_1 562 GCCATTCTGGCGATCCCCGAGCAGCAGACGAAGGTGGGGATCATCTCCCCGGTGCTGCTTCCGGACTATGTCGCCCTGCTGCCGGAGCTG 651 0 361 .......................................................................................... 450 0 757 ........ 764 T T S M P P S I A A S T G I D A L C H L L E C F T S T V A N Query_1 652 ACCACCAGCATGCCGCCCAGCATCGCGGCGTCGACCGGAATCGATGCTCTGTGTCATCTGCTGGAGTGCTTTACCTCCACCGTCGCCAAC 741 0 451 .......................................................................................... 540 P V S D N A A L I G L H K L V R H I E R S V N Q P Q D L T A Query_1 742 CCGGTGAGCGATAACGCGGCGTTAATCGGTTTACACAAGCTGGTACGCCATATCGAGCGTTCGGTAAATCAGCCGCAGGATCTGACGGCG 831 0 541 .......................................................................................... 630 K L E M L W A S W Y G G A A I N Y S G T H L V H A L S Y P L Query_1 832 AAGCTGGAGATGCTCTGGGCCTCGTGGTACGGCGGCGCAGCGATCAACTATTCCGGCACTCACCTGGTGCATGCGCTCTCCTATCCGCTT 921 0 631 .......................................................................................... 720 G G T W H L P H G V A N A I L L A P C M R V V R P H A V A K Query_1 922 GGCGGTACCTGGCACCTGCCGCACGGGGTGGCCAACGCCATTCTACTGGCGCCCTGCATGCGGGTGGTCCGCCCTCACGCGGTGGCGAAG 1011 0 721 .......................................................................................... 810 0 358 ........... 368 F A Q V W D L I P D A D R T L S A E E K S H A L V A W L A A Query_1 1012 TTCGCTCAAGTCTGGGACCTGATCCCCGATGCAGATCGCACGCTCAGCGCGGAAGAGAAATCCCATGCGCTGGTCGCATGGCTGGCGGCG 1101 0 811 .......................................................................................... 900 L V K R L P L P D N L A A L G V P Q E S I S A L S A A A Q N Query_1 1102 CTGGTCAAACGACTGCCGCTGCCGGACAACCTGGCCGCGCTAGGCGTGCCTCAGGAGAGTATTTCCGCCCTCAGCGCAGCGGCGCAAAAC 1191 0 901 .......................................................................................... 990 V K R L M N N A P C S V S R E E I A A I Y Q T L Y P E H A Query_1 1192 GTCAAACGCCTGATGAACAATGCCCCATGCAGCGTCAGCCGCGAAGAGATCGCGGCCATCTACCAAACGCTGTATCCAGAACATGCC 1278 0 991 ....................................................................................... 1077 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1528614 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:51 PM Number of letters in database: 1,087 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96931.103 (IDP96931.1-494.F..pMCSG73)
MEPFLSLRHVSKTFHANRALNDISIDFMPGEVHCLAGQNGCGKSTLIKIISGVYRPDEGAAMTLGGKTWPKLTPAASVAHGIQVIYQDLSLFPNLSVWENIAINHYHRGVLVRRSELRRSAEQAMRSINVSLPLDAAVESLSIARCQLVAICRALAQDARLIIMDEPTASLTHQEVEGLLHVVRQLRESGICIVFVSHRLEEVMAVSDRISVLKDGNLVGTMPAAEVTTRRLGFLMTGQEFDFQVRDLWQGQGSTPVLEVRNLSRKGEYHNVSLKVEAGEVVSIVGLLGAGRTELCLSLFGMTRPDSGEILINGQPVRLRSNQDAIRHGIGYVSEDRMSRGLVMSQSIENNIISTVFHKVKGACGLLSDSKAQQLVARMVNALTIKAPDTHLPVNTLSGGNAQRVSIAKWLAISPKLLILDSPTVGVDIANKAGIYHIISDLAAHGIAVLMICDEIEEAWYQSHRILVMKQGELTHSFLPDSSTQQQIAEVVNG
ATGGAACCTTTCCTTTCCCTTCGACACGTCAGCAAAACCTTCCACGCCAACCGCGCGCTCAATGATATCAGCATCGACTTTATGCCCGGAGAAGTGCACTGCCTGGCCGGGCAGAACGGTTGCGGGAAGTCGACGTTGATCAAAATTATCTCTGGCGTCTATCGCCCGGATGAGGGCGCAGCGATGACGCTGGGCGGCAAGACGTGGCCCAAACTGACGCCAGCGGCCTCTGTCGCCCATGGGATCCAGGTGATTTATCAGGATCTGTCGCTGTTTCCCAATCTCAGCGTGTGGGAGAACATTGCTATAAACCACTATCATCGCGGCGTGCTGGTGAGGCGCAGCGAACTGCGACGCAGTGCGGAGCAGGCGATGCGCAGTATTAACGTCTCGCTGCCGCTGGATGCCGCCGTGGAATCGCTCTCCATCGCTCGCTGTCAGCTGGTGGCTATCTGCCGCGCGCTGGCGCAGGATGCCCGGCTTATCATTATGGATGAACCCACGGCCTCATTGACCCATCAGGAAGTGGAGGGACTATTGCATGTGGTGCGCCAGCTGCGTGAGAGTGGGATCTGCATCGTCTTCGTAAGCCACCGTCTGGAAGAGGTCATGGCCGTATCGGACCGCATCAGCGTGCTGAAGGATGGCAATCTGGTCGGCACCATGCCGGCGGCAGAGGTAACCACCCGCCGGCTCGGTTTTCTGATGACGGGCCAGGAGTTTGACTTTCAGGTGCGCGATCTGTGGCAGGGGCAGGGCAGTACGCCGGTGCTGGAGGTGCGCAATCTCAGCCGCAAAGGGGAATATCATAACGTCTCCCTGAAGGTCGAAGCCGGAGAAGTGGTGTCGATAGTCGGCCTGTTGGGCGCCGGGCGAACAGAGCTGTGCCTGAGCCTTTTCGGCATGACCCGCCCTGACAGCGGCGAGATCCTGATAAATGGCCAGCCGGTGCGGTTGCGTAGCAATCAGGACGCTATCCGCCACGGTATCGGCTATGTCTCCGAGGACCGTATGTCGCGCGGCCTGGTGATGAGTCAGTCGATTGAAAACAACATTATCAGCACCGTGTTTCATAAAGTGAAAGGCGCTTGCGGACTATTGAGTGATAGTAAAGCTCAGCAGCTGGTCGCAAGAATGGTCAACGCGTTGACCATTAAGGCGCCGGATACGCATCTGCCGGTGAATACCCTCTCCGGTGGTAATGCCCAGCGCGTTTCTATCGCCAAATGGCTGGCGATCTCTCCTAAGCTGCTGATCCTCGATAGCCCAACCGTGGGGGTGGATATCGCAAACAAAGCCGGGATCTATCATATCATCAGCGATTTGGCTGCCCATGGTATCGCCGTGCTGATGATTTGCGACGAGATTGAAGAGGCGTGGTACCAGAGTCATCGCATCCTGGTGATGAAGCAGGGCGAGTTGACCCATAGCTTCCTGCCGGACAGCAGTACCCAGCAGCAGATAGCGGAGGTGGTCAATGGC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,056 total letters Query= IDP96931.103 Length=1887 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2673 0.0 2_03C_C7_IDP96931.103_v73F.seq 1777 0.0 1_01C_C7_IDP96931.103_T7Term.seq 1734 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 2 42 ...........A........G....------....................... 89 2 24 .....C........... 40 A M E P F L S L R H V S K T F H A N R A L N D I S I D F M P Query_1 199 GCCATGGAACCTTTCCTTTCCCTTCGACACGTCAGCAAAACCTTCCACGCCAACCGCGCGCTCAATGATATCAGCATCGACTTTATGCCC 288 0 1 ....................................................................................... 87 0 833 .. 834 2 90 .......................................................................................... 179 2 926 .... 929 1 714 .. 713 G E V H C L A G Q N G C G K S T L I K I I S G V Y R P D E G Query_1 289 GGAGAAGTGCACTGCCTGGCCGGGCAGAACGGTTGCGGGAAGTCGACGTTGATCAAAATTATCTCTGGCGTCTATCGCCCGGATGAGGGC 378 0 88 .......................................................................................... 177 0 835 ......... 843 2 180 .......................................................................................... 269 2 930 ......... 938 1 712 ......... 704 A A M T L G G K T W P K L T P A A S V A H G I Q V I Y Q D Query_1 379 GCAGCGATGACGCTGGGCGGCAAGACGTGGCCCAAACTGACGCCAGCGGCCTCTGTCGCCCATGGGATCCAGGTGATTTATCAGGATCT- 467 0 178 .........................................................................................- 266 0 939 ..............C 925 2 270 .........................................................................................- 358 2 976 ........... 966 1 608 ..............C 622 L S L F P N L S V W E N I A I N H Y H R G V L V R R S E L R Query_1 468 GTCGCTGTTTCCCAATCTCAGCGTGTGGGAGAACATTGCTATAAACCACTATCATCGCGGCGTGCTGGTGAGGCGCAGCGAACTGCGACG 557 0 267 .......................................................................................... 356 0 924 .C...... 917 2 359 .......................................................................................... 448 1 623 .C...... 630 R S A E Q A M R S I N V S L P L D A A V E S L S I A R C Q L Query_1 558 CAGTGCGGAGCAGGCGATGCGCAGTATTAACGTCTCGCTGCCGCTGGATGCCGCCGTGGAATCGCTCTCCATCGCTCGCTGT-CAGC-TG 645 0 357 ..................................................................................-....-.. 444 2 449 ..................................................................................-....-.. 536 1 1140 ........T....AN. 1125 V A I C R A L A Q D A R L I I M D E P T A S L T H Q Query_1 646 GTGGCTATCTG--CCGCGCGCT-GGCGCAGG--ATGCCCG-GCTTATCATTA-TGGATG-AACCCACGG-CCTC-ATTGACCCATC-AGG 724 0 445 ...........--.........-........--.......-...........-......-.........-....-...........-... 523 2 537 ...........--.........-........--.......-...........-......-.........-....-...........-... 615 1 1124 ........T.NGN.........C......A.GA.......N...N...CN..A......G.........G....C...........N... 1035 E V E G L L H V V R Q L R E S G I C I V F V S H R L E E Query_1 725 AAGTGG-AGGGACTATTGCATGTGGTGCGCC-AGCTGCGTGAGAGTGGGAT-CTGCATCGTCTT-CGTAAGCCACCGTCTGG-AAGAGGT 809 0 524 ......-........................-...................-............-.................-....... 608 2 616 ......-........................-...................-............-.................-....... 700 1 1034 ......N........................N...................TN...........TNN...............N....... 945 V M A V S D R I S V L K D G N L V G T M P A A E V T T R Query_1 810 CATGGCCGTATCGGACCGCATC--AGCGTGCTGAAGG-ATGGCAATCTGGTC-GGCACCATG-CCGGCGGCAGAGGTAACCACCCGCC-G 893 0 609 ......................--.............-..............-.........-.........................-. 692 2 701 ......................--.............-..............-.........C.........................-. 785 1 944 .........N....N......TCA...........N.N..............N.........-.N.......................C. 856 R L G F L M T G Q E F D F Q V R D L W Q G Q G S T P V L E V Query_1 894 GCTCGG-TTTTCTGATGACGGGCCAGG-AGTTTGACTTTCAGGTGCGCGATCTGTGGCAGGGGCAGGGCAGTACGCCGGTGCTGGAGGTG 981 0 693 ......-....................-.............................................................. 780 2 786 ......T....................-.............................................................. 874 1 855 ......-....................N.............................................................. 767 R N L S R K G E Y H N V S L K V E A G E V V S I V G L L G Query_1 982 CGCAATCTCAGCCGCAAAGGGGAATATCATAACGTCTCCCTGAAGGTCGAAG-CCGGAGAAGTGGTGTCGATAGTCGGCCTGTTGGGCGC 1070 0 781 ....................................................-..................................... 869 0 86 ........... 96 2 875 .................G..................................C..............N...................... 964 2 178 ........... 188 1 766 ....................................................-..................................... 678 A G R T E L C L S L F G M T R P D S G E I L I N G Q P V Query_1 1071 CGGGCGAACAGAGCTGTG-CCTGAGCCTTTTCGGCATGACCCGCCC-TGACAGCGGC-GAGAT-CCTGATAAATGG-CCAGCCGGTG-CG 1154 0 870 ..................-...........................-..........-.....-............-..........-.. 953 0 274 ......A.--....-.......... 253 2 965 ......-...........C...........................T..........N.....C......N.....C.......A..C.. 1053 2 366 ......A.--....-.......... 345 1 677 ..................-...........................-..........-.....-............-.......A..-.. 594 R L R S N Q D A I R H G I G Y V S E D R M S R G L V Query_1 1155 GTTGCGTAGCAATCA-GGACGC-TATCC--GCCACGG-TATCGGC-TATGTCTCCGAGGACCGTA-TGTC--GCGCG-GCCTGGTG-AT- 1232 0 954 ...............-......-.....--.......-.......-...................-....--.....-........-..- 1031 0 1398 .......-..- 1406 2 1054 ......A........G......T...N.CG.......T.......T...........-.N.....T....CNN....NN.....N.N..G 1142 1 593 ...............-......-.....--.......-.......-...................-....--.....-........-..- 516 1 149 .......-..- 141 M S Q S I E N N I I S T V F H K V K G A C G L L S D S K A Query_1 1233 GAGTCAG-TCGATTGAAAACAA--CATTATCAGCACCGTGTTTCATAAAGTGAAAGGCGCTTGCGGACTATTGAGTGATAGTAAAGCTCA 1319 0 1032 .......-..............--.................................................................. 1118 0 1407 .. 1408 2 1143 .......T..N...........AC..... 1171 1 515 .......-..............--.................................................................. 429 1 140 .. 139 Q Q L V A R M V N A L T I K A P D T H L P V N T L S G G N A Query_1 1320 GCAGCTGGTCGCAAGAATGGTCAACGCGTTGACCATTAAGGCGCCGGATACGCATCTGCCGGTGAATACCCTCTCCGGTGGTAATGCCCA 1409 0 1119 .......................................................................................... 1208 0 1155 ...................... 1134 1 428 .......................................................................................... 339 1 392 ...................... 413 Q R V S I A K W L A I S P K L L I L D S P T V G V D I A N K Query_1 1410 GCGCGTTTCTATCGCCAAATGGCTGGCGATCTCTCCTAAGCTGCTGATCCTCGATAGCCCAACCGTGGGGGTGGATATCGCAAACAAAGC 1499 0 1209 .......................................................................................... 1298 1 338 .......................................................................................... 249 A G I Y H I I S D L A A H G I A V L M I C D E I E E A W Y Q Query_1 1500 CGGGATCTATCATATCATCAGCGATTTGGCTGCCCATGGTATCGCCGTGCTGATGATTTGCGACGAGATTGAAGAGGCGTGGTACCAGAG 1589 0 1299 .......................................................................................... 1388 1 248 .......................................................................................... 159 S H R I L V M K Q G E L T H S F L P D S S T Q Q Q I A E V V Query_1 1590 TCATCGCATCCTGGTGATGAAGCAGGGCGAGTTGACCCATAGCTTCCTGCCGGACAGCAGTACCCAGCAGCAGATAGCGGAGGTGGTCAA 1679 0 1389 .......................................................................................... 1478 0 1023 ........... 1033 1 158 .......................................................................................... 69 1 524 ........... 514 N G * H W K W I T D P N S S S V D K L A A A L E H H H H H H Query_1 1680 TGGCTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTG 1769 0 1479 .... 1482 1 68 ........................--..... 40 * D P A A N K A R Query_1 1770 AGATCCGGCTGCTAACAAAGCCCGAAA 1796 1 44 ....................N.... 20 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 7489350 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:51 PM Number of letters in database: 4,056 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96935.103 (IDP96935.1-147.F..pMCSG73)
MATPGIFGVSPAKERTRRAKKGLVDGYQSLLRMSMNVELHTEQGVKIIVPLVRRLDASVVQAFKQQVLEAIDPDTKNVLLDLNHVDFIDSSCLGALVSILKSVNGQGELALCSLNDTIKNLFKLTRMDRIFAIKETRQDALNAFTSL
GTGGCTACGCCCGGGATATTTGGGGTATCACCAGCTAAAGAGCGAACACGCCGGGCTAAAAAGGGACTCGTCGACGGATATCAGTCACTATTGAGGATGAGCATGAACGTTGAATTACATACCGAGCAGGGCGTAAAAATTATTGTACCTCTTGTGCGCCGACTGGATGCTTCCGTCGTGCAGGCGTTTAAGCAGCAGGTTCTGGAGGCTATCGACCCGGATACCAAAAACGTGCTGCTGGATCTGAACCACGTCGATTTCATCGACAGTAGCTGCCTTGGCGCGCTGGTTTCCATTCTGAAAAGCGTCAACGGTCAGGGCGAGCTGGCGCTGTGTTCACTCAATGACACGATTAAAAATCTGTTTAAGCTCACCCGCATGGATCGCATCTTTGCCATTAAGGAGACCCGACAGGATGCGTTAAACGCGTTCACCTCGTTA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,931 total letters Query= IDP96935.103 Length=846 Score E Sequences producing significant alignments: (Bits) Value 2_03D_D7_IDP96935.103_v73F.seq 895 0.0 1_02H_D7_IDP96935.103_T7Term.seq 895 0.0 0_wt 796 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 2 41 ...........A........G....------....................... 88 2 23 .....C........... 39 1 556 ...........A........G....------....................... 509 1 574 .....C........... 558 A V A T P G I F G V S P A K E R T R R A K K G L V D G Y Q S Query_1 199 GCCGTGGCTACGCCCGGGATATTTGGGGTATCACCAGCTAAAGAGCGAACACGCCGGGCTAAAAAGGGACTCGTCGACGGATATCAGTCA 288 2 89 ...A...................................................................................... 178 1 508 ...A...................................................................................... 419 0 1 ....................................................................................... 87 L L R M S M N V E L H T E Q G V K I I V P L V R R L D A S V Query_1 289 CTATTGAGGATGAGCATGAACGTTGAATTACATACCGAGCAGGGCGTAAAAATTATTGTACCTCTTGTGCGCCGACTGGATGCTTCCGTC 378 2 179 .......................................................................................... 268 1 418 .......................................................................................... 329 0 88 .......................................................................................... 177 V Q A F K Q Q V L E A I D P D T K N V L L D L N H V D F I D Query_1 379 GTGCAGGCGTTTAAGCAGCAGGTTCTGGAGGCTATCGACCCGGATACCAAAAACGTGCTGCTGGATCTGAACCACGTCGATTTCATCGAC 468 2 269 .......................................................................................... 358 1 328 .......................................................................................... 239 0 178 .......................................................................................... 267 S S C L G A L V S I L K S V N G Q G E L A L C S L N D T I K Query_1 469 AGTAGCTGCCTTGGCGCGCTGGTTTCCATTCTGAAAAGCGTCAACGGTCAGGGCGAGCTGGCGCTGTGTTCACTCAATGACACGATTAAA 558 2 359 .......................................................................................... 448 1 238 .......................................................................................... 149 0 268 .......................................................................................... 357 N L F K L T R M D R I F A I K E T R Q D A L N A F T S L * H Query_1 559 AATCTGTTTAAGCTCACCCGCATGGATCGCATCTTTGCCATTAAGGAGACCCGACAGGATGCGTTAAACGCGTTCACCTCGTTATAACAT 648 2 449 .......................................................................................... 538 1 148 .......................................................................................... 59 0 358 .................................................................................... 441 W K W I T D P N S S S V D K L A A A L E H H H H H H * D P A Query_1 649 TGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCT 738 2 539 ..............--..... 557 2 553 ........ 560 1 58 ..............--..... 40 1 44 ........ 37 A N K A R K E A E L A A A T A E Q * L A * P L G A S K R V L Query_1 739 GCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTG 828 2 561 .......................................................................................... 650 1 36 ..................... 16 R G F L L K Query_1 829 AGGGGTTTTTTGCTGAAA 846 2 651 .................. 668 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2398266 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:51 PM Number of letters in database: 2,931 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96937.103 (IDP96937.1-269.F..pMCSG73)
MTNMKLKFDLLLKSYHLSHRFVYKANPGNAGDGVIASATYDFFERNALTYIPYRDGERYSSETDILIFGGGGNLIEGLYSEGHDFIQNNIGKFHKVIIMPSTIRGYSDLFINNIDKFVVFCRENITFDYIKSLNYEPNKNVFITDDMAFYLDLNKYLSLKPVYKKQANCFRTDSESLTGDYKENNHDISLTWNGDYWDNEFLARNSTRCMINFLEEYKVVNTDRLHVAILASLLGKEVNFYPNSYYKNEAVYNYSLFNRYPKTCFITAS
ATGACCAATATGAAGTTAAAATTTGATTTGCTTCTAAAATCTTATCATCTATCTCATCGATTTGTCTATAAGGCAAACCCTGGTAATGCTGGTGATGGTGTAATTGCATCTGCGACGTATGACTTTTTTGAACGAAATGCTCTTACCTATATCCCTTACAGAGATGGCGAGCGCTACAGTTCTGAAACTGATATTTTAATTTTTGGAGGCGGAGGAAACCTGATAGAAGGATTGTATTCTGAAGGTCATGACTTTATCCAGAATAATATTGGGAAGTTTCATAAAGTAATAATAATGCCGTCGACAATCAGAGGGTATAGCGATTTATTCATCAACAATATTGATAAGTTTGTTGTTTTTTGTCGCGAAAATATCACCTTCGATTATATTAAATCTCTCAACTACGAACCAAACAAGAACGTATTCATTACTGATGATATGGCATTTTATCTCGATCTTAATAAATACCTGTCACTTAAACCCGTCTATAAAAAACAGGCCAACTGCTTCAGAACGGACTCCGAATCTCTAACTGGAGACTACAAAGAAAACAATCATGATATTTCGCTCACCTGGAATGGCGATTATTGGGATAATGAATTTCTGGCGCGTAATTCTACCCGTTGCATGATAAACTTTCTTGAAGAGTATAAAGTTGTCAATACCGACAGGCTGCATGTGGCAATTTTAGCATCTCTGCTTGGCAAAGAAGTCAACTTCTATCCTAACTCATATTACAAAAATGAAGCTGTTTACAATTATTCACTTTTTAATCGTTATCCAAAAACATGCTTTATTACGGCAAGT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,279 total letters Query= IDP96937.103 Length=1212 Score E Sequences producing significant alignments: (Bits) Value 2_03E_E7_IDP96937.103_v73F.seq 1544 0.0 1_01E_E7_IDP96937.103_T7Term.seq 1541 0.0 0_wt 1456 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAAT-CCAA 197 2 41 ...........A........G....------..................-.... 87 2 23 .....C........... 39 1 922 ...........A........G....AG.-----..........-.....N.... 875 1 940 .....C........... 924 N A M T N M K L K F D L L L K S Y H L S H R F V Y K A N P G Query_1 198 TGCCATGACCAATATGAAGTTAAAATTTGATTTGCTTCTAAAATCTTATCATCTATCTCATCGATTTGTCTATAAGGCAAACCCTGGTAA 287 2 88 .......................................................................................... 177 1 874 .......................................................................................... 785 0 1 ...................................................................................... 86 N A G D G V I A S A T Y D F F E R N A L T Y I P Y R D G E R Query_1 288 TGCTGGTGATGGTGTAATTGCATCTGCGACGTATGACTTTTTTGAACGAAATGCTCTTACCTATATCCCTTACAGAGATGGCGAGCGCTA 377 2 178 .......................................................................................... 267 1 784 .......................................................................................... 695 0 87 .......................................................................................... 176 Y S S E T D I L I F G G G G N L I E G L Y S E G H D F I Q N Query_1 378 CAGTTCTGAAACTGATATTTTAATTTTTGGAGGCGGAGGAAACCTGATAGAAGGATTGTATTCTGAAGGTCATGACTTTATCCAGAATAA 467 2 268 .......................................................................................... 357 1 694 .......................................................................................... 605 0 177 .......................................................................................... 266 N I G K F H K V I I M P S T I R G Y S D L F I N N I D K F V Query_1 468 TATTGGGAAGTTTCATAAAGTAATAATAATGCCGTCGACAATCAGAGGGTATAGCGATTTATTCATCAACAATATTGATAAGTTTGTTGT 557 2 358 .......................................................................................... 447 1 604 .......................................................................................... 515 0 267 .......................................................................................... 356 V F C R E N I T F D Y I K S L N Y E P N K N V F I T D D M A Query_1 558 TTTTTGTCGCGAAAATATCACCTTCGATTATATTAAATCTCTCAACTACGAACCAAACAAGAACGTATTCATTACTGATGATATGGCATT 647 2 448 .......................................................................................... 537 1 514 .......................................................................................... 425 0 357 .......................................................................................... 446 F Y L D L N K Y L S L K P V Y K K Q A N C F R T D S E S L T Query_1 648 TTATCTCGATCTTAATAAATACCTGTCACTTAAACCCGTCTATAAAAAACAGGCCAACTGCTTCAGAACGGACTCCGAATCTCTAACTGG 737 2 538 .......................................................................................... 627 1 424 .......................................................................................... 335 0 447 .......................................................................................... 536 G D Y K E N N H D I S L T W N G D Y W D N E F L A R N S T R Query_1 738 AGACTACAAAGAAAACAATCATGATATTTCGCTCACCTGGAATGGCGATTATTGGGATAATGAATTTCTGGCGCGTAATTCTACCCGTTG 827 2 628 .......................................................................................... 717 1 334 .......................................................................................... 245 0 537 .......................................................................................... 626 C M I N F L E E Y K V V N T D R L H V A I L A S L L G K E V Query_1 828 CATGATAAACTTTCTTGAAGAGTATAAAGTTGTCAATACCGACAGGCTGCATGTGGCAATTTTAGCATCTCTGCTTGGCAAAGAAGTCAA 917 2 718 .......................................................................................... 807 1 244 .......................................................................................... 155 0 627 .......................................................................................... 716 N F Y P N S Y Y K N E A V Y N Y S L F N R Y P K T C F I T A Query_1 918 CTTCTATCCTAACTCATATTACAAAAATGAAGCTGTTTACAATTATTCACTTTTTAATCGTTATCCAAAAACATGCTTTATTACGGCAAG 1007 2 808 ........................................-.........................-....................... 895 1 154 .......................................................................................... 65 0 717 .......................................................................................... 806 S * H W K W I T D P N S S S V D K L A A A L E H H H H H H * Query_1 1008 TTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGA 1097 2 896 .....................--..... 921 2 917 . 917 1 64 .....................--..... 39 1 43 . 43 0 807 . 807 D P A A N K A R K E A E L A A A T A E Q * L A * P L G A S K Query_1 1098 TCCGGC-TGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGC-TGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAA 1185 2 918 ......C....................................C.........................NT................N.. 1007 1 42 ......-................... 18 R V L R G F L L Query_1 1186 -CGGGTCTTGAGGGGTTTTTTGCTGAA 1211 2 1008 C......C................... 1034 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3864276 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:51 PM Number of letters in database: 3,279 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96938.102 (IDP96938.1-328.F..pMCSG68)
MSKMIAVTMGDPAGIGPEIIIKSLAEGALSGAPVVVVGCAQTLRRILALNITPRAELRIIDHPAEASFSPATINVIDEPLSDPQGLRPGEVQAQAGDLAFRCIRRATALALEGAVAAIATAPLNKEALHLAGHAYPGHTELLAHLTQTTDYAMVLYTEKLKVIHITTHISLRQFLDTLNQPRIETVIGVADRFLRRVGYPRPRIAVAGVNPHAGENGLFGDEEIRIVAPAVAAMRAKGVEVTGPCPPDTVFMQCHEGMYDMVVAMYHDQGHIPLKLLGFYDGVNITAGLPFIRTSADHGTAFDIAWTGKAKSESMATSIELAMHIAQE
GTGTCTAAGATGATTGCAGTGACAATGGGCGACCCGGCAGGGATTGGTCCGGAGATTATTATCAAATCCCTCGCCGAGGGGGCGCTGAGCGGCGCGCCGGTGGTGGTGGTGGGCTGCGCGCAGACGCTGCGGCGGATCCTGGCGCTAAATATCACACCGCGGGCGGAGCTGCGCATTATTGACCATCCCGCCGAGGCGAGTTTTTCCCCAGCGACTATCAACGTCATTGATGAACCACTCAGCGACCCGCAGGGGCTGCGCCCTGGCGAAGTCCAGGCCCAGGCAGGGGATCTCGCGTTTCGCTGCATCAGGCGGGCGACCGCGCTGGCGCTGGAGGGCGCGGTCGCGGCCATCGCTACCGCGCCGCTGAATAAAGAGGCGCTGCATCTCGCCGGGCACGCTTATCCCGGCCATACCGAACTGCTGGCCCACCTGACGCAGACTACCGATTACGCCATGGTGCTCTATACCGAGAAGCTTAAGGTTATCCATATCACTACCCATATTTCCTTGCGCCAGTTCCTCGATACCCTCAACCAGCCGCGGATTGAGACGGTGATTGGCGTGGCGGATCGCTTTCTGCGCCGCGTCGGCTATCCACGTCCGCGCATCGCGGTCGCCGGCGTTAACCCGCACGCCGGGGAGAACGGCCTGTTTGGCGATGAAGAGATCCGTATCGTCGCCCCGGCGGTCGCCGCCATGCGGGCGAAGGGAGTGGAGGTGACCGGCCCCTGTCCGCCGGACACGGTGTTTATGCAGTGTCACGAAGGCATGTACGACATGGTGGTGGCGATGTATCACGACCAGGGGCATATTCCGCTGAAGCTGCTGGGATTCTATGACGGGGTGAATATTACCGCCGGGCTGCCGTTTATCCGTACCTCCGCGGATCATGGCACTGCGTTTGATATTGCCTGGACAGGTAAAGCGAAGTCTGAGAGCATGGCAACCTCCATTGAGCTCGCCATGCACATCGCGCAGGAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 994 total letters Query= IDP96938.102 Length=1389 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1775 0.0 V S K M I A V T M G D P A G I G P E I I I K S L A E G A L S Query_1 202 GTGTCTAAGATGATTGCAGTGACAATGGGCGACCCGGCAGGGATTGGTCCGGAGATTATTATCAAATCCCTCGCCGAGGGGGCGCTGAGC 291 0 1 .......................................................................................... 90 G A P V V V V G C A Q T L R R I L A L N I T P R A E L R I I Query_1 292 GGCGCGCCGGTGGTGGTGGTGGGCTGCGCGCAGACGCTGCGGCGGATCCTGGCGCTAAATATCACACCGCGGGCGGAGCTGCGCATTATT 381 0 91 .......................................................................................... 180 D H P A E A S F S P A T I N V I D E P L S D P Q G L R P G E Query_1 382 GACCATCCCGCCGAGGCGAGTTTTTCCCCAGCGACTATCAACGTCATTGATGAACCACTCAGCGACCCGCAGGGGCTGCGCCCTGGCGAA 471 0 181 .......................................................................................... 270 V Q A Q A G D L A F R C I R R A T A L A L E G A V A A I A T Query_1 472 GTCCAGGCCCAGGCAGGGGATCTCGCGTTTCGCTGCATCAGGCGGGCGACCGCGCTGGCGCTGGAGGGCGCGGTCGCGGCCATCGCTACC 561 0 271 .......................................................................................... 360 A P L N K E A L H L A G H A Y P G H T E L L A H L T Q T T D Query_1 562 GCGCCGCTGAATAAAGAGGCGCTGCATCTCGCCGGGCACGCTTATCCCGGCCATACCGAACTGCTGGCCCACCTGACGCAGACTACCGAT 651 0 361 .......................................................................................... 450 Y A M V L Y T E K L K V I H I T T H I S L R Q F L D T L N Q Query_1 652 TACGCCATGGTGCTCTATACCGAGAAGCTTAAGGTTATCCATATCACTACCCATATTTCCTTGCGCCAGTTCCTCGATACCCTCAACCAG 741 0 451 .......................................................................................... 540 P R I E T V I G V A D R F L R R V G Y P R P R I A V A G V N Query_1 742 CCGCGGATTGAGACGGTGATTGGCGTGGCGGATCGCTTTCTGCGCCGCGTCGGCTATCCACGTCCGCGCATCGCGGTCGCCGGCGTTAAC 831 0 541 .......................................................................................... 630 P H A G E N G L F G D E E I R I V A P A V A A M R A K G V E Query_1 832 CCGCACGCCGGGGAGAACGGCCTGTTTGGCGATGAAGAGATCCGTATCGTCGCCCCGGCGGTCGCCGCCATGCGGGCGAAGGGAGTGGAG 921 0 631 .......................................................................................... 720 V T G P C P P D T V F M Q C H E G M Y D M V V A M Y H D Q G Query_1 922 GTGACCGGCCCCTGTCCGCCGGACACGGTGTTTATGCAGTGTCACGAAGGCATGTACGACATGGTGGTGGCGATGTATCACGACCAGGGG 1011 0 721 .......................................................................................... 810 H I P L K L L G F Y D G V N I T A G L P F I R T S A D H G T Query_1 1012 CATATTCCGCTGAAGCTGCTGGGATTCTATGACGGGGTGAATATTACCGCCGGGCTGCCGTTTATCCGTACCTCCGCGGATCATGGCACT 1101 0 811 .......................................................................................... 900 A F D I A W T G K A K S E S M A T S I E L A M H I A Q E Query_1 1102 GCGTTTGATATTGCCTGGACAGGTAAAGCGAAGTCTGAGAGCATGGCAACCTCCATTGAGCTCGCCATGCACATCGCGCAGGAA 1185 0 901 .................................................................................... 984 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1309000 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:51 PM Number of letters in database: 994 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96940.103 (IDP96940.1-329.F..pMCSG73)
MKDYGRNELCQVIISSHSSSILSRIEPEQIRHFRLDQISKSTVVSALTLPEKEDELSKFIKEAVKSYPEIYFSKLVIFGEGDSEEIIIPKVLELYSTEIDSHSISVVPLGGRHVNHFWRLLKSLQIPFITLLDFDIDRNGGGFGRLRYAIEQLSIFCGGKYGKTKEKIPAWDDERDPTTFKLPRTDGDSYNVVAALENHNIYFSSPLDIDYAMIEAFPDIFCEKDVVYGERGPKNIEQEEQEEQKKELINAVLKKGNSGVRYEFSVDYLKYFLWYRYRFLSNKSKPASHVRMFSKIEEKYTPDEIREKLPPVLIRVADKTVSLLAEVIE
GTGAAAGATTATGGTCGTAATGAGCTGTGCCAAGTTATTATTTCTTCCCATTCATCTTCCATTTTAAGCCGAATCGAGCCCGAGCAAATACGTCACTTTAGATTGGATCAGATATCAAAAAGCACGGTAGTAAGCGCATTAACGCTACCGGAAAAAGAAGACGAGCTTTCTAAGTTTATTAAAGAGGCAGTTAAATCATACCCTGAAATCTATTTTTCTAAACTGGTGATATTTGGCGAAGGGGATAGTGAGGAAATTATAATTCCAAAGGTGCTTGAGCTGTATTCGACGGAGATTGATAGCCATTCTATCTCTGTTGTTCCTCTTGGTGGACGTCATGTGAATCATTTTTGGCGTCTGTTGAAGTCATTACAGATTCCCTTTATCACTTTACTGGATTTCGATATCGATAGAAATGGCGGTGGGTTCGGCCGACTTAGATATGCTATTGAGCAACTGTCTATTTTCTGTGGTGGAAAATATGGCAAGACAAAAGAAAAAATACCTGCATGGGATGACGAACGAGATCCAACAACATTTAAACTTCCGCGTACTGACGGTGATTCATATAATGTTGTGGCTGCGTTGGAGAATCATAATATTTATTTTTCATCGCCTCTAGATATCGATTACGCAATGATTGAAGCATTCCCTGATATTTTCTGTGAAAAAGATGTGGTCTATGGGGAAAGAGGGCCTAAAAATATTGAACAGGAAGAACAGGAAGAACAAAAAAAAGAGCTGATAAATGCAGTATTGAAAAAAGGCAACTCCGGCGTCCGTTACGAATTTTCTGTTGATTATCTAAAATATTTCCTGTGGTATCGCTATCGCTTTTTATCCAATAAAAGTAAGCCAGCCTCTCATGTTAGAATGTTTTCTAAAATTGAAGAAAAATATACGCCGGATGAAATCAGAGAAAAATTGCCTCCAGTGTTAATTCGGGTAGCTGACAAGACTGTATCGTTATTGGCGGAGGTTATTGAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,365 total letters Query= IDP96940.103 Length=1392 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1781 0.0 1_01F_F7_IDP96940.103_T7Term.seq 1730 0.0 2_03F_F7_IDP96940.103_v73F.seq 1645 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 1 1082 .....N.NN..G.........N 1061 2 43 ...........A........G....------....................... 90 2 28 .............. 41 2 28 ..C........... 41 A V K D Y G R N E L C Q V I I S S H S S S I L S R I E P E Query_1 199 GCCG-TGAAA-GATTATGGTCGTAATGAGCTGTGCCAAGTTATTA-TTTCTTCCCATTCATCTTCCATTTTAAGCCGAATCGAGCCCGAG 285 0 1 .-.....-..................................-............................................ 84 1 1060 .G.AA.....N...A.....N.NN...NNG............N..A......T..CA................................. 971 2 91 ...A-.....-..................................-............................................ 177 Q I R H F R L D Q I S K S T V V S A L T L P E K E D E L S K Query_1 286 CAAATACGTCACTTTAGATTGGATCAGATATCAAAAAGCACGGTAGTAAGCGCATTAACGCTACCGGAAAAAGAAGACGAGCTTTCTAAG 375 0 85 .......................................................................................... 174 1 970 ......N................................................................................... 881 2 178 .......................................................................................... 267 F I K E A V K S Y P E I Y F S K L V I F G E G D S E E I I Query_1 376 TTTATTAAAGAGGCAGTTAAATCATACCCTGAAATCTATTTTTCTAAACTGGTGATATTTGGCGAA-GGGGATAGTGAGGAAATTATAAT 464 0 175 ..................................................................-....................... 263 1 880 ........................................................N..-......A....................... 792 2 268 ..................................................................-....................... 356 I P K V L E L Y S T E I D S H S I S V V P L G G R H V N H F Query_1 465 TCCAAAGGTGCTTGAGCTGTATTCGACGGAGATTGATAGCCATTCTATCTCTGTTGTTCCTCTTGGTGGACGTCATGTGAATCATTTTTG 554 0 264 .......................................................................................... 353 1 791 ................................................N......................................... 702 2 357 .......................................................................................... 446 W R L L K S L Q I P F I T L L D F D I D R N G G G F G R L R Query_1 555 GCGTCTGTTGAAGTCATTACAGATTCCCTTTATCACTTTACTGGATTTCGATATCGATAGAAATGGCGGTGGGTTCGGCCGACTTAGATA 644 0 354 .......................................................................................... 443 1 701 ..................................................G....................................... 612 2 447 ..................................................G....................................... 536 Y A I E Q L S I F C G G K Y G K T K E K I P A W D D E R D P Query_1 645 TGCTATTGAGCAACTGTCTATTTTCTGTGGTGGAAAATATGGCAAGACAAAAGAAAAAATACCTGCATGGGATGACGAACGAGATCCAAC 734 0 444 .......................................................................................... 533 0 657 ........... 667 1 611 .......................................................................................... 522 1 398 ........... 388 2 537 .......................................................................................... 626 T T F K L P R T D G D S Y N V V A A L E N H N I Y F S S P L Query_1 735 AACATTTAAACTT-CCGCGTACTGACGGTGATTCATATAATGTTGTGGCTGCGTTGGAGAATCATAATATTTATTTTTCATCGCCTCTAG 823 0 534 .............-............................................................................ 622 1 521 .............-............................................................................ 433 2 627 .............C............................................................................ 716 D I D Y A M I E A F P D I F C E K D V V Y G E R G P K N I E Query_1 824 ATATCGATTACGCAATGATTGAAGCATTCCCTGATA-TTTTCTGTGAAAAAGATGTGGTCTATGGGGAAAGAGGGCCTAAAAATATTGAA 912 0 623 ....................................-..................................................... 711 0 462 ..-......... 472 1 432 ....................................-..................................................... 344 1 593 ..-......... 583 2 717 ....................................T..................................................... 806 2 555 ..-......... 565 Q E E Q E E Q K K E L I N A V L K K G N S G V R Y E F S V D Query_1 913 CAGGAAGAACAGGAAGAACaaaaaaaaGAGCTGATAAATGCAGTATTGAAAAAAGGCAACTCCGGCGTCCGTTACGAATTTTCTGTTGAT 1002 0 712 .......................................................................................... 801 1 343 .................................................-........................................ 255 2 807 ...................-.....................N......-....................NN................... 894 Y L K Y F L W Y R Y R F L S N K S K P A S H V R M F S K Query_1 1003 TATCTAAAATATTTCCTGTGGTATCGCTAT-CGCTTTTTATCCAAT-AAAAGTAAGCC-AGCCTC-TCATGTTAGAA-TGTTTTCTAAAA 1087 0 802 ..............................-...............-...........-......-...........-............ 886 1 254 ..............................-...............-...........-......-...........-............ 170 2 895 ..............................C...............A...........N...N..T.........N.T...N........ 984 I E E K Y T P D E I R E K L P P V L I R V A D K T V S L Query_1 1088 TTGAAGAAAAATATACGCC-GGAT-GAAATCAGAGAAAAATT-GCCTCCAGTGTTAATT-CGGGTA-GCTGACAAGAC-TGTATCGTTAT 1171 0 887 ...................-....-.................-................-......-...........-........... 970 1 169 ...................-....-.................-................-......-...........-........... 86 2 985 ....N...........N..G....C......GN......N..G..T.....N.......N....A.NN..........N....... 1070 L A E V I E * H W K W I T D P N S S S V D K L A A A L E H H Query_1 1172 TGGCGGAGGTTATTGAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccacc 1261 0 971 ................. 987 1 85 ............N........................--..... 44 H H H H * D P A A N K A R K E Query_1 1262 accaccaccacTGAGATCCGGCTGCTAACAAAGCCCG-AAAGGAA 1305 1 48 ....................N.A....... 19 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4564192 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 2, 2018 12:51 PM Number of letters in database: 3,365 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2