Clone ID: IDP02219.104 (IDP02219.1-516.F..pMCSG68)
MTDIHNHKILILDFGSQYTQLIARRVREVGVFCEIFPHDVAADFIKNYQAKGIILSGGPESVYDSDVKAPEIVFELGVPVLGICYGMQTMVMQHGGEVKGADQSEFGKAIINILNSTNNIFSNMEHEQLVWMSHSDKVTQTGEHFEIIASSTNAPVAAVAHKNKPFFGVQFHPETTHTENGKQIIENFVVNICGCDTLWNIENIIENDIKEIKQKVGTDKVILGLSGGVDSSVVAAILHQAIGDQLTCIFVDTGLLRLNEGDQVMQVFAEHMDINVIRINAKNRFLDALRGICDPEQKRKIIGKLFVDIFDEEAAKIENAKWLAQGTIYSDVIESAGNNQSKAHVIKSHHNVGGLPKEMKLKLLEPLRELFKDEVRKLGLGLGLPYNMLYRHPFPGPGLGVRILGEIKKEYVETLQKADAIFTEELYKHNLYHDVSQAFGVFLPVKSVGVVGDQRRYEYVIALRAVVSIDFMTATWANLPYDFLSLVSNRIVNEVKQVSRVVYDVTGKPPGTIEWE
ATGACAGATATACATAATCATAAGATTTTGATTTTAGATTTTGGCTCGCAATATACACAACTTATTGCTCGTAGAGTAAGAGAAGTAGGTGTTTTTTGTGAAATTTTCCCTCATGATGTAGCAGCTGATTTTATCAAAAATTATCAAGCAAAAGGAATAATTTTATCAGGTGGTCCTGAGTCAGTTTATGATTCTGATGTCAAAGCTCCTGAGATAGTTTTTGAGCTTGGAGTACCTGTTTTGGGTATTTGTTATGGCATGCAAACAATGGTAATGCAGCATGGTGGCGAAGTAAAAGGAGCTGATCAAAGTGAATTTGGTAAGGCAATTATAAATATCCTAAATTCAACAAATAACATATTTTCAAATATGGAGCATGAGCAGCTAGTTTGGATGAGTCATAGTGACAAAGTCACTCAAACTGGTGAGCATTTTGAGATAATTGCTTCTTCAACAAATGCTCCAGTTGCGGCAGTTGCACATAAAAATAAGCCTTTTTTTGGTGTGCAGTTTCACCCAGAAACTACTCATACAGAAAATGGTAAGCAAATTATTGAGAATTTTGTCGTTAATATCTGCGGATGTGATACTCTGTGGAATATCGAAAACATTATCGAAAATGATATAAAAGAAATTAAACAAAAAGTTGGTACTGATAAAGTAATTTTAGGGCTATCTGGAGGTGTTGATTCATCTGTTGTAGCTGCGATATTACATCAAGCTATCGGCGATCAGCTAACTTGTATATTTGTAGATACTGGTTTACTGCGCCTTAATGAGGGTGATCAAGTTATGCAAGTATTTGCAGAGCATATGGATATTAATGTTATTCGTATCAATGCAAAAAATAGATTCTTAGATGCCCTAAGGGGTATCTGTGATCCAGAACAAAAACGTAAAATTATCGGTAAGCTATTTGTTGATATTTTTGATGAAGAGGCTGCTAAGATAGAGAATGCTAAATGGCTTGCGCAGGGCACTATCTACAGTGATGTAATTGAATCAGCTGGCAATAATCAGTCTAAAGCGCATGTAATTAAATCACATCATAATGTTGGTGGCTTACCAAAAGAAATGAAACTTAAGCTTTTAGAACCATTAAGAGAGCTTTTCAAAGATGAGGTTCGTAAATTAGGCTTAGGTTTAGGATTACCTTATAATATGCTTTATAGACATCCATTTCCTGGTCCTGGTCTTGGAGTACGTATATTAGGTGAAATAAAGAAAGAGTATGTAGAAACGTTACAAAAAGCTGATGCTATATTTACAGAAGAGCTCTATAAACATAATTTATACCATGATGTTTCTCAAGCTTTTGGGGTGTTTTTACCTGTCAAATCGGTAGGTGTAGTTGGTGATCAGCGTAGATATGAGTATGTAATAGCTCTAAGAGCTGTCGTTAGTATCGATTTTATGACTGCAACGTGGGCTAATTTACCTTATGATTTTCTGTCACTAGTTTCAAATAGAATTGTAAATGAAGTAAAACAAGTATCAAGAGTTGTGTATGATGTGACAGGAAAACCACCTGGAACAATTGAATGGGAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,285 total letters Query= IDP02219.104 Length=1953 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2792 0.0 2_09G_G1_IDP02219.104_T7term.seq 1171 0.0 1_05G_G1_IDP02219.104_T7S.seq 1061 0.0 D I H M H H H H H H S S G V D L W S H P Q F E K G T E N L Query_1 96 AGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTA 185 0 6 ........... 16 1 52 ........... 62 Y F Q S N A M T D I H N H K I L I L D F G S Q Y T Q L I A R Query_1 186 CTTCCAATCCAATGCCATGACAGATATACATAATC-ATAAGATTTTGATTTTAGATTTTGGCTCGCAATATACACAACTTATTGCTCG-T 273 0 1 ...................-....................................................-. 72 1 47 .................N.C......N.............................................C. 120 R V R E V G V F C E I F P H D V A A D F I K N Y Q A K G I I Query_1 274 AGAGTAAGAGAAGTAGGTGTTTTTTGTGAAATTTTCCCTCATGATGTAGCAGCTGATTTTATCAAAAATTATCAAGCAAAAGGAATAATT 363 0 73 .......................................................................................... 162 1 121 ........................N........N........................................................ 210 L S G G P E S V Y D S D V K A P E I V F E L G V P V L G I C Query_1 364 TTATCAGGTGGTCCTGAGTCAGTTTATGATTCTGATGTCAAAGCTCCTGAGATAGTTTTTGAGCTTGGAGTACCTGTTTTGGGTATTTGT 453 0 163 .......................................................................................... 252 1 211 ...........N.............................................................................. 300 Y G M Q T M V M Q H G G E V K G A D Q S E F G K A I I N I L Query_1 454 TATGGCATGCAAACAATGGTAATGCAGCATGGTGGCGAAGTAAAAGGAGCTGATCAAAGTGAATTTGGTAAGGCAATTATAAATATCCTA 543 0 253 .......................................................................................... 342 1 301 ..........................N..........................................N.................... 390 N S T N N I F S N M E H E Q L V W M S H S D K V T Q T G E H Query_1 544 AATTCAACAAATAACATATTTTCAAATATGGAGCATGAGCAGCTAGTTTGGATGAGTCATAGTGACAAAGTCACTCAAACTGGTGAGCAT 633 0 343 .......................................................................................... 432 1 391 ........................................N...................................N............. 480 F E I I A S S T N A P V A A V A H K N K P F F G V Q F H P E Query_1 634 TTTGAGATAATTGCTTCTTCAACAAATGCTCCAGTTGCGGCAGTTGCACATAAAAATAAGCCtttttttGGTGTGCAGTTTCACCCAGAA 723 0 433 .......................................................................................... 522 1 481 ..N.......N...................................................................N........... 570 T T H T E N G K Q I I E N F V V N I C G C D T L W N I E N Query_1 724 ACTACTCATACAGAAAATGGTAAGCAAATTATTGAGAATTTTGTCGTTAATATCTGCGGATGTGATACTCTGT-GGAATATCGAA-AACA 811 0 523 .........................................................................-...........-.... 610 1 571 ...N............................................N..............N.........G...........NN... 660 I I E N D I K E I K Q K V G T D K V I L G L S G G V D S Query_1 812 TTATCGAAAATGATATAAAAGAAATTAAAC-AAAAAGTT--GGTACTGATAAAGTA-ATTTTAGGGCTAT-CTGGA-GGTGTTGATTCAT 895 0 611 ..............................-........--...............-.............-.....-............. 694 1 661 .........NN........C...N...N..NNNN.....TG.NCC.N.N.......NNN.........N.N.....N..C..C.... 747 S V V A A I L H Q A I G D Q L T C I F V D T G L L R L N E Query_1 896 CTGTTGTAGCTGCGATATTACATCAAGCTATCGGCGATCAGCTAACTTGTATATTTGTAGAT-ACTGGTTTAC-TGCGCCTTAA-TGAGG 982 0 695 ..............................................................-..........-..........-..... 781 2 872 ....N..N....NN.N...A..N...A..... 841 G D Q V M Q V F A E H M D I N V I R I N A K N R F L Query_1 983 G--TGATC-AAGTTATGCAAGT-ATTTGCA-GAGCAT-ATGG-ATATT-AATGTTATT--CGTATCAATGCAAAAAA-TAGA-TTCT-TA 1059 0 782 .--.....-.............-.......-......-....-.....-.........--.................-....-....-.. 858 2 840 .GGN..N.N...NNN...N...T...A..NN......G....G.N..NN.......N.TNN.N.....N........A...NN....N.. 751 D A L R G I C D P E Q K R K I I G K L F V D I F D E E A Query_1 1060 GATGCCCTAAGGGGTATC-TGTG-ATCCA-GAACAAAAAC-GTAA-AATTATCGGTAAGCTATTTGTTGATATTTTTGATGAAGAGGCTG 1144 0 859 ..................-....-.....-..........-....-............................................ 943 2 750 .................TA....N.....A........N.C....N..........G............N.................... 661 A K I E N A K W L A Q G T I Y S D V I E S A G N N Q S K A H Query_1 1145 CTAAGATAGAGAATGCTAAATGGCTTGCGCAGGGCACTATCTACAGTGATGTAATTGAATCAGCTGGCAATAATCAGTCTAAAGCGCATG 1234 0 944 .......................................................................................... 1033 2 660 ..............N....................................N.......................N.............. 571 V I K S H H N V G G L P K E M K L K L L E P L R E L F K D E Query_1 1235 TAATTAAATCACATCATAATGTTGGTGGCTTACCAAAAGAAATGAAACTTAAGCTTTTAGAACCATTAAGAGAGCTTTTCAAAGATGAGG 1324 0 1034 .......................................................................................... 1123 2 570 ..........N.......................N....................................................... 481 V R K L G L G L G L P Y N M L Y R H P F P G P G L G V R I L Query_1 1325 TTCGTAAATTAGGCTTAGGTTTAGGATTACCTTATAATATGCTTTATAG-ACATCCATTTCCTGGTCCTGGTCTTGGAGTACGTATATTA 1413 0 1124 .................................................-........................................ 1212 2 480 ............................N....................A........................................ 391 G E I K K E Y V E T L Q K A D A I F T E E L Y K H N L Y H D Query_1 1414 GGTGAAATAAAGAAAGAGTATGTAGAAACGTTACAAAAAGCTGATGCTATATTTACAGAAGAGCTCTATAAACATAATTTATACCATGAT 1503 0 1213 .......................................................................................... 1302 2 390 .......................................................................................... 301 V S Q A F G V F L P V K S V G V V G D Q R R Y E Y V I A L R Query_1 1504 GTTTCTCAAGCTTTTGGGGTGTTTTTACCTGTCAAATCGGTAGGTGTAGTTGGTGATCAGCGTAGATATGAGTATGTAATAGCTCTAAGA 1593 0 1303 .......................................................................................... 1392 2 300 .......................................................................................... 211 A V V S I D F M T A T W A N L P Y D F L S L V S N R I V N Query_1 1594 GCTGTCGTTAGTATC-GATTTTATGACTGCAACGTGGGCTAATTTACCTTATGATTTTCTGTCACTAGTTTCAAATAGAATTGTAAATGA 1682 0 1393 ...............-.......................................................................... 1481 2 210 ...............N.......................................................................... 121 E V K Q V S R V V Y D V T G K P P G T I E W E * H W K W I T Query_1 1683 AGT-AAAACAAGTATCAAGAGTTGTGTATGATGTGACAGGAAAACCACCTGGAACAATTGAATGGGAATAACATTGGAAGTGGATAACGG 1771 0 1482 ...-................................................................ 1548 2 120 ...A......T......................N............N.....................C.T... 47 D P N S S S V D K L A A A L E H H H H H H * D P A A N K A R Query_1 1772 ATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAA 1861 2 55 ..A..T..T.....T.....-.A.........-....C--......N.. 11 K Query_1 1862 AG 1863 2 10 .. 9 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 8197024 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 13, 2018 11:15 AM Number of letters in database: 4,285 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP04043.104 (IDP04043.284-499.P(499)..pMCSG68)
SKRQEMRSRMLILQILTHELRTPIASLSLTVEGFRREFEHLPESLYDEFRRLCEDSRRLRQLAEASKDYLQSDSKPLASDWVPSVEEWLQYKVEEEFSGNVTLKLNQDIAAKLNVYWLGTCVDNLLRNAVKYGVAPVTLEVITQTNLVTFKVTDQGSLTHRDWRHLRKPFVSKSGLGLGLTIVESMVGRMGGKMSLEGPPTTFILEIPCETDTASR
TCAAAACGACAAGAAATGCGCAGTCGGATGCTCATTTTGCAAATTCTCACCCATGAGCTGCGAACCCCAATCGCGAGCTTGTCACTCACCGTTGAAGGCTTTCGACGTGAGTTTGAGCATTTGCCCGAATCTCTGTACGATGAGTTTCGTCGATTGTGTGAAGATTCCCGACGATTGCGCCAACTCGCCGAAGCGAGTAAAGACTATTTGCAGTCTGACAGTAAACCACTCGCGTCAGATTGGGTTCCTTCGGTGGAGGAGTGGCTACAATATAAAGTCGAAGAAGAATTTAGTGGTAATGTGACTCTGAAACTTAATCAAGATATCGCTGCCAAACTCAATGTTTATTGGCTAGGAACTTGTGTGGATAATTTATTGCGTAACGCTGTCAAATACGGCGTTGCACCTGTCACTCTCGAAGTGATAACACAAACTAACTTAGTCACTTTCAAAGTGACCGACCAAGGCTCGTTGACTCACCGTGATTGGCGTCATTTACGCAAACCATTTGTGAGCAAAAGCGGCTTAGGCCTTGGATTGACCATTGTCGAATCAATGGTGGGACGAATGGGCGGAAAAATGTCACTCGAAGGCCCACCGACAACTTTTATTTTGGAGATACCTTGTGAAACAGACACTGCTTCTCGT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,912 total letters Query= IDP04043.104 Length=1053 Score E Sequences producing significant alignments: (Bits) Value 1_06F_F2_IDP04043.104_T7S.seq 1519 0.0 0_wt 1169 0.0 2_10F_F2_IDP04043.104_T7term.seq 389 2e-111 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 58 TTCCCCT--CTAGAAAT-AATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTG 144 1 5 ......NTC........N..........N............................................................. 94 W S H P Q F E K G T E N L Y F Q S N A S K R Q E M R S R M L Query_1 145 TGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCTCAAAACGACAAGAAATGCGCAGTCGGATGCTC 234 1 95 .......................................................................................... 184 0 1 ................................. 33 2 766 ...N.N..........NN.NN..A.. 741 I L Q I L T H E L R T P I A S L S L T V E G F R R E F E H Query_1 235 ATTTTGCAAATTCTCACCCATGAGCTGCGAACCCCAATCGCGAGCTTGTCACTCA-CCGTTGAAGGCTTTCGACGTGAGTTTGAGCATTT 323 1 185 .......................................................-.................................. 273 0 34 .......................................................-.................................. 122 2 740 ..N.........N.NNT....N.NGA...NNGNN...NAAT.N.N.....NN...TN..N.....NNN..TNN..N...NN.N.....NN 651 L P E S L Y D E F R R L C E D S R R L R Q L A E A S K D Y L Query_1 324 GCCCGAATCTCTGTACGATGAGTTTCGTCGATTGTGTGAAGATTCCCGACGATTGCGCCAACTCGCCGAAGCGAGTAAAGACTATTTGCA 413 1 274 .......................................................................................... 363 0 123 .......................................................................................... 212 2 650 T..G....NNT.N..N.....NN.......TN............NNT.C...NNN.N........T..N......N..N..N....NN.. 561 Q S D S K P L A S D W V P S V E E W L Q Y K V E E E F S G N Query_1 414 GTCTGACAGTAAACCACTCGCGTCAGATTGGGTTCCTTCGGTGGAGGAGTGGCTACAATATAAAGTCGAAGAAGAATTTAGTGGTAATGT 503 1 364 .......................................................................................... 453 0 213 .......................................................................................... 302 2 560 ..NNT...NN..N....NNNT...NNN.N......N.N..NN..N....N..N.N...N..N....N.N.......NN....N..N..NN 471 V T L K L N Q D I A A K L N V Y W L G T C V D N L L R N A V Query_1 504 GACTCTGAAACTTAATCAAGATATCGCTGCCAAACTCAATGTTTATTGGCTAGGAACTTGTGTGGATAATTTATTGCGTAACGCTGTCAA 593 1 454 .......................................................................................... 543 0 303 .......................................................................................... 392 2 470 .NN.A.NNNNNN..N...NN.N..N.N.....NNN....N...NT...N....N.GN.N...N...N...N.....N.N.N...N...NN 381 K Y G V A P V T L E V I T Q T N L V T F K V T D Q G S L T H Query_1 594 ATACGGCGTTGCACCTGTCACTCTCGAAGTGATAACACAAACTAACTTAGTCAC-TTTCAAAGTGACCGACCAAGGCTCGTTGACTCACC 682 1 544 ......................................................T................................... 633 0 393 ......................................................-................................... 481 2 380 ...A........N...C..NNN..T.N..N...N....C.C...NN......NN-N.NNNNN...G.N.C....NNN.NN...N...CN. 292 R D W R H L R K P F V S K S G L G L G L T I V E S M V G R M Query_1 683 GTGATTGGCGTCATTTACGCAAACCA-TTTGTGAGCAAAAGCGGCTTAGGCCTTGGATTGACCATTGTCGAATCAATGGTGGGACGAATG 771 1 634 ..........................T............................................................... 723 0 482 ..........................-............................................................... 570 2 291 .N...NNN....N.CNT.N.N.C...-NCN........N..NN..NN..T...NNN..NNN....C..N.N....NN.N....GN...N. 203 G G K M S L E G P P T T F I L E I P C E T D T A S R * H W Query_1 772 GGCGGAAAAATGTCACTCGAAGGCCCACCGACAACTTTTATTTTGGAGATACCTTGTGA-AACAGACACTGCTTCTCGTTA-ACATTGGA 859 1 724 .N.........................................................-T....................-........ 811 0 571 ...........................................................-................... 648 2 202 NNN..NNN..NN..NNNN.N....N.N...N.N.................G...NN...G.....C.N.C......N....G....C... 113 K W I T D P N S S S V D K L A A A L E H H H H H H * D P A Query_1 860 AGTGGATA-ACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTC-GAGcaccaccaccaccacc-acTGAGAT-CCGGCT 945 1 812 ........-......T..............................-.......-.................. 881 2 112 ..N...N.G......NN....N....N.......T.N.N.N...........C.N...................A........A...... 23 A N K A Query_1 946 GCTAACAAAGCCC 958 2 22 ..-N..C...... 11 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2975946 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 13, 2018 11:15 AM Number of letters in database: 2,912 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP05151.102 (IDP05151.21-397.P(397)..pMCSG68)
GADKDEKVVEKSGKAKEQVIVPNYSISDEYYKTTIPFEGGNARGLVVQGVSNRLDIDEFETGLMRIAKESFSTKDNVLKGGKSLTTQYIQMLVKRKRTDAEQKELEDTLKKDAVKFPNIGLNPALGAGSESLEAKNKKNPIYISNILEHDYYVNKDKKDERSGIVVGLAMNSVHYYEEEHGYPREVTISNEKMLAEGKKMVPEILKVMHQQDPETKNLPITFAIYRQAPKSSLVPGNFVSYGNVEKGSETVEDWKQINEKYYLFPSEQAKTDNKREDLARVSNFKAKLSDYFQGDYTAVIGTGMYRDDELREIKLDIPVQFNGKAEIIGFTQYVAGLVMEYFPNYMKVQVTIKSVERPEAIIIREAKQDEPYVKILD
GGTGCCGATAAAGATGAAAAAGTGGTTGAGAAATCGGGGAAAGCAAAAGAGCAAGTAATCGTCCCAAATTACTCTATTTCGGATGAATATTATAAGACGACTATTCCATTTGAAGGAGGAAATGCACGTGGCTTAGTTGTGCAAGGAGTAAGTAATCGTCTTGATATAGATGAATTTGAAACAGGATTAATGCGTATTGCGAAAGAATCATTTAGTACGAAAGATAATGTTTTAAAAGGCGGAAAGTCCCTAACTACTCAGTATATACAGATGCTTGTTAAAAGAAAACGTACAGATGCAGAACAAAAGGAACTAGAAGATACATTAAAAAAAGATGCGGTTAAATTCCCGAACATAGGGTTAAATCCCGCATTAGGTGCAGGATCCGAATCATTAGAGGCGAAAAATAAAAAAAATCCAATATATATTTCCAATATTTTAGAACATGATTATTATGTGAACAAAGACAAAAAAGATGAGCGTAGTGGTATTGTAGTTGGATTAGCGATGAATTCGGTTCATTATTATGAGGAAGAGCATGGGTATCCGCGTGAGGTTACAATTTCGAATGAAAAGATGTTAGCTGAAGGGAAAAAAATGGTGCCAGAAATCTTGAAAGTTATGCATCAACAAGATCCTGAGACAAAAAACCTTCCGATAACATTTGCGATTTATCGTCAGGCTCCAAAGTCTTCGCTCGTACCAGGTAACTTTGTTTCTTATGGGAATGTCGAAAAAGGTAGTGAAACGGTTGAGGATTGGAAACAAATTAATGAGAAATATTATTTGTTCCCATCTGAACAAGCGAAAACAGATAATAAACGTGAAGACTTGGCGAGAGTATCAAACTTCAAGGCAAAATTAAGCGATTATTTCCAAGGTGACTATACAGCTGTTATCGGTACTGGTATGTATAGAGACGATGAATTAAGAGAAATAAAGCTTGATATTCCTGTCCAGTTTAATGGGAAAGCAGAAATAATTGGCTTTACACAATATGTAGCCGGGCTTGTTATGGAATATTTCCCGAATTATATGAAAGTACAAGTAACGATTAAATCTGTAGAACGTCCAGAAGCGATTATTATACGTGAAGCGAAGCAAGATGAACCGTATGTGAAAATTTTAGAT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,079 total letters Query= IDP05151.102 Length=1536 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2040 0.0 2_09H_H1_IDP05151.102_T7term.seq 872 0.0 1_05H_H1_IDP05151.102_T7S.seq 509 2e-147 F V * L * E G D I H M H H H H H H S S G V D L W S H P Q F Query_1 75 TTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGA 164 1 36 .........-..N.CC..T....T...........TT.T........N............G.N-...................-....NN 122 E K G T E N L Y F Q S N A G A D K D E K V V E K S G K A K E Query_1 165 AAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGGTGCCGATAAAGATGAAAAAGTGGTTGAGAAATCGGGGAAAGCAAAAGAGCA 254 0 1 ..................................................... 53 0 143 ..G...T. 150 1 123 T.-..TN........................................N-.....T..-...TN...T........TT............. 209 Q V I V P N Y S I S D E Y Y K T T I P F E G G N A R G L V V Query_1 255 AGTAATCGTCCCAAATTACTCTATTTCGGATGAATATTATAAGACGACTATTCCATTTGAAGGAGGAAATGCACGTGGCTTAGTTGTGCA 344 0 54 .......................................................................................... 143 0 46 . 46 0 151 .......... 160 1 210 ............N......................T..T...................N...........................C... 299 1 202 . 202 Q G V S N R L D I D E F E T G L M R I A K E S F S T K D N V Query_1 345 AGGAGTAAGTAATCGTCTTGATATAGATGAATTTGAAACAGGATTAATGCGTATTGCGAAAGAATCATTTAGTACGAAAGATAATGTTTT 434 0 144 .......................................................................................... 233 0 47 .A...C........... 63 1 300 ..........-..............N..............N...............TC...N.........N...N.............. 388 1 203 .A...C........... 219 L K G G K S L T T Q Y I Q M L V K R K R T D A E Q K E L E D Query_1 435 AAAAGGCGGAAAGTCCCTAACTACTCAGTATATACAGATGCTTGTTAAAAGAAAACGTACAGATGCAGAACAAAAGGAACTAGAAGATAC 524 0 234 .......................................................................................... 323 1 389 ......T..T................-T.................. 433 T L K K D A V K F P N I G L N P A L G A G S E S L E A K N K Query_1 525 ATTaaaaaaaGATGCGGTTAAATTCCCGAACATAGGGTTAAATCCCGCATTAGGTGCAGGATCCGAATCATTAGAGGCGaaaaataaaaa 614 0 324 .......................................................................................... 413 K N P I Y I S N I L E H D Y Y V N K D K K D E R S G I V V G Query_1 615 aaaTCCAATATATATTTCCAATATTTTAGAACATGATTATTATGTGAACAAAGACAAAAAAGATGAGCGTAGTGGTATTGTAGTTGGATT 704 0 414 .......................................................................................... 503 L A M N S V H Y Y E E E H G Y P R E V T I S N E K M L A E Query_1 705 AGCGATGAATTCGGTTCATTATTATGAGGAAGAGCATGGGTATCCGCGTGAGGTTACAATTTCGAATGAAAA--GATGTT-AGC-TGAAG 790 0 504 ........................................................................--......-...-..... 589 2 726 ........NG......N...C...G. 701 G K K M V P E I L K V M H Q Q D P E T K N L P I T F A I Query_1 791 GG--aaaaaaaTGGTGCCAGAAATCTTGAAA-GTTATGCATCAAC--AAGATCCTGAGACAAAAAA-CCTTCCGATAACATTTGCGATTT 874 0 590 ..--...........................-.............--...................-....................... 673 2 700 ..GA...N...N.............N.....A...........NGCA...G...............T....................... 611 Y R Q A P K S S L V P G N F V S Y G N V E K G S E T V E D Query_1 875 ATCGTCAGGCTCCA-AAGTCTTCGCTCGTACC-AGGTAA-CTTTGTTTCTTATGGGAATGTCGAAAAAGGTAGTGAAACGGTTGAGGATT 961 0 674 ..............-.................-......-.................................................. 760 2 610 .............NN.................C......A..........N..........N.C................N......... 521 W K Q I N E K Y Y L F P S E Q A K T D N K R E D L A R V S N Query_1 962 GGAAACA-AATTAATGAGAAATATTATTTGTTCCCATCTGAACAAGCGAAAACAGATAATAAACGTGAAGACTTGGCGAGAGTATCAAAC 1050 0 761 .......-.................................................................................. 849 2 520 .......N....................................G.....N.........N..............N..........N... 431 F K A K L S D Y F Q G D Y T A V I G T G M Y R D D E L R E I Query_1 1051 TTCAAGGCAAAATTAAGCGATTATTTCCAAGGTGACTATACAGCTGTTATCGGTACTGGTATGTATAGAGACGATGAATTAAGAGAAATA 1140 0 850 .......................................................................................... 939 0 1076 ........... 1086 2 430 ..........NN..........................................C..................N.........N.....G 341 2 202 ........... 192 K L D I P V Q F N G K A E I I G F T Q Y V A G L V M E Y F P Query_1 1141 AAGCTTGATATTCCTGTCCAGTTTAATGGGAAAGCAGAAATAATTGGCTTTACACAATATGTAGCCGGGCTTGTTATGGAATATTTCCCG 1230 0 940 .......................................................................................... 1029 2 340 .......................................................................................... 251 N Y M K V Q V T I K S V E R P E A I I I R E A K Q D E P Y Query_1 1231 AATTATATGAAAGTACAAGT-AACGATTAAATCTGTAGAA-CGTCCAGAAGCGATTATTATACGTGAAGCGAAGCAAGATGAA-CCGTAT 1317 0 1030 ....................-...................-..........................................-...... 1116 0 864 ........... 874 2 250 ......N.............A...................G..........................................N...... 161 2 416 ........... 406 V K I L D * H W K W I T D P N S S S V D K L A A A L Query_1 1318 GTGAAAATTTTAGATTAA-CA-TTGGAA-GTGGATAACGGATCCGAATT-CG-AGCT-CCGTCGA-CAAG--CTT-GCGGCCGCACT-CG 1396 0 1117 ............... 1131 2 160 .................NG..C......G....................A..A....G......NT.T.NGA...G...........A.. 71 E H H H H H H * D P A A N K A R K Query_1 1397 A--Gcaccacca-ccaccaccac--TGAGAT--CCGGCTGCTAA--CAAAGCCCGAAAGG 1447 2 70 .TGA.G..T...N..N..CT...NN......TC........NG.NC...G....CN.... 11 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4607120 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 13, 2018 11:15 AM Number of letters in database: 3,079 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95839.102 (IDP95839.1-342.F..pMCSG68)
MFSIRLADLAQQLNAQLHGDGDITIAGLASMGLANGEQITFLSDSRYREKLSECQAAAVVLTEADLPFCPVAALVVKNPYLAYAQMAQIMDTTPAPAQDIHPSAVIAVDAKLGNNVSIGANAVIESGVELGNNVVIGAGCFIGKKAHIGDNSRLWANVSVYHEVIIGKDCLVQSGTVIGSDGFGYANERGNWIKIPQLGSVIIGDRVEIGACTTIDRGALDNTVIGNGVIIDNQCQIAHNVIIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGHMEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKAWRKTAALVLRIDEMQKRLKSLERQVENSDK
ATGTTTTCAATTCGATTAGCTGATCTTGCTCAGCAATTGAATGCACAGTTACACGGCGATGGTGATATCACTATCGCCGGTCTTGCTTCAATGGGCTTAGCAAATGGTGAACAAATTACCTTTCTTTCCGATAGTCGCTATCGTGAAAAATTAAGTGAATGTCAGGCCGCTGCGGTGGTATTAACAGAGGCTGATCTTCCTTTTTGTCCTGTTGCTGCACTGGTGGTGAAAAATCCTTATCTGGCTTATGCACAAATGGCTCAGATTATGGATACTACGCCCGCGCCAGCACAAGATATACATCCTTCTGCTGTTATTGCCGTTGATGCTAAATTAGGGAATAACGTCTCAATTGGTGCCAATGCGGTCATTGAGTCAGGTGTTGAGTTAGGTAATAACGTAGTTATTGGTGCAGGATGTTTTATTGGTAAAAAAGCACATATCGGCGATAATAGCCGTTTGTGGGCAAATGTCTCTGTTTATCATGAAGTTATCATTGGTAAAGATTGTTTAGTACAATCTGGTACTGTCATTGGTTCTGATGGTTTTGGTTATGCCAATGAGCGTGGCAATTGGATTAAAATTCCTCAGTTAGGCTCAGTGATCATTGGTGACCGTGTCGAAATTGGTGCATGTACTACCATCGATAGAGGCGCATTAGATAACACAGTTATCGGTAATGGTGTTATCATTGATAACCAGTGCCAAATCGCACATAACGTCATTATTGGTGACAATACTGCTGTGGCAGGTGGTGTCATCATGGCGGGGAGCCTGAAGATTGGGCGTTACTGTATGATTGGTGGAGCAAGTGTTATCAATGGTCACATGGAAATTTGTGATAAAGTTACGGTAACAGGAATGGGGATGGTTATGCGTCCTATCACTGAACCTGGGGTTTATTCTTCTGGCATTCCGTTACAACCGAATAAAGCATGGCGTAAAACCGCAGCACTGGTGCTGCGAATTGATGAAATGCAAAAAAGGCTAAAGTCGCTAGAACGTCAAGTAGAAAATAGCGACAAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,463 total letters Query= IDP95839.102 Length=1431 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1851 0.0 2_11B_B3_IDP95839.102_T7term.seq 1433 0.0 1_07B_B3_IDP95839.102_T7S.seq 1227 0.0 N N F V * L * E G D I H M H H H H H H S S G V D L W S H P Query_1 70 AATAATTTTGTTTAACTTTAAGAAGGAGATATACATA-TGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCA 158 1 13 ..N.G.....N.........N................C.................................................... 102 Q F E K G T E N L Y F Q S N A M F S I R L A D L A Q Q L N A Query_1 159 G-TTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGTTTTCAATTCGATTAGCTGATCTTGCTCAGCAATTGAATGCAC 247 0 1 .............................................. 46 1 103 .A........................................................................................ 192 Q L H G D G D I T I A G L A S M G L A N G E Q I T F L Query_1 248 AGTTACACGGCGATGGTGATATCACTATCGCCGG-TCTTGCTTC-AATGGGCTTA--GCAAATG-GTGA-ACAAATTACC--TTTCTTTC 329 0 47 ..................................-.........-..........--.......-....-..........--........ 128 2 1114 ....N...G....NN..G....N..AG.....N.N...GCN.......A.NT...T.... 1055 1 193 ..................................-.........-..........--.......-....-..........--........ 274 S D S R Y R E K L S E C Q A A A V V L T E A D L P Query_1 330 CGATA-GTCGCTA---TCGTGAAAAATT-AAGTG--AATGTCAGG--CCGCTGCGG-TGGTA-TTAACAGA--GGCTGATCTTCC--TTT 404 0 129 .....-.......---............-.....--.........--.........-.....-........--............--... 203 2 1054 ....NN...-.N.NNT.N...N..N...N...N.GA........CGC....G.N..G...N.N.......NAG..NANN....N.CN... 966 1 275 .....-.......---............-.....--.........--.........-.....-........--............--... 349 F C P V A A L V V K N P Y L A Y A Q M A Q I M D T T P A Query_1 405 TTGTCCTGTTGCTGC-ACTGGTGGTGAAAAA-TCCTT-ATCTGGCTTATG-CACAAATGGCTCAG-ATTATGGATACTACGCCCG--CGC 487 0 204 ...............-...............-.....-............-..............-...................--... 286 2 965 ..N...C.G...N..CN.N..GANN......A.....T..N......N..G..N...........N..........N..N....CGC... 876 1 350 ...............-...............-.....-............-..............-...................--... 432 P A Q D I H P S A V I A V D A K L G N N V S I G A N A Query_1 488 CAGCA-CAA-GATATACATCCTTC-TGC-TGTT-ATTGCC-GTTGATGCTAAA-TTAGGG-AATAACGTCTCAA-TTGGTGCCAATGCGG 568 0 287 .....-...-..............-...-....-......-............-......-.............-............... 367 2 875 .....N...N.....G.......NN..NN....N..N...C....TN......A......G.............A.....N......... 786 1 433 .....-...-..............-...-....-......-............-......-..G..........-............... 513 V I E S G V E L G N N V V I G A G C F I G K K A H I G D Query_1 569 -TCATTG-AGTCAGG-TGTTGAGTTAGGTAATAACGTAGTTATTGGTGCAGGATGTTTTATT-GGTAAAAAAGCACATATC-GGCGATAA 653 0 368 -......-.......-..............................................-..................-........ 452 2 785 G......N.......G....N.........................................-N.................C........ 697 1 514 -......-.......-.......N..............N.......................N..................-........ 599 N S R L W A N V S V Y H E V I I G K D C L V Q S G T V I G Query_1 654 -TAGCCGTTTGTGGGCAAATGTCTCTG-TTTATCATGAAGTTATCATTGGTAAAGATT-GT-TTAGTACAATCTGGTACTGTCATTGGTT 739 0 453 -..........................-..............................-..-............................ 538 2 696 A..........................-..............................-..N............................ 609 1 600 -..........................T..............................N..-..........................N. 687 S D G F G Y A N E R G N W I K I P Q L G S V I I G D Query_1 740 -CTG-ATGGTTTTGGTTATGCCAATGAGC-GTGGCAA--TTGGATTAAAATT-CCTCAG-TTAGGCT-CAGTG-ATCATT-GGTGA-CCG 818 0 539 -...-........................-.......--.............-......-.......-.....-......-.....-... 617 2 608 -...-........................-.......--.............-......-.......-.....-......-.....-... 530 1 688 C...A...................N....N.......AT.G...........C......T.......C.....A......N.N...NN.. 777 R V E I G A C T T I D R G A L D N T V I G N G V I I D N Query_1 819 TGTCGAAA--TTGGTGCATGTACTACC-AT-CGATA-GAGGCGCA-TTAG-ATAACACAGTTATCGGTAATGGTGTTATCATTGATAACC 901 0 618 ........--.................-..-.....-........-....-....................................... 700 2 529 ........--.................-..-.....-........-....-....................................... 447 1 778 ....N...AT.G..C..N...N..N..T..NN....C.....N..T....A.C...... 836 Q C Q I A H N V I I G D N T A V A G G V I M A G S L K I G R Query_1 902 AGTGCCAAATCGCACATAACGTCATTATTGGTGACAATACTGCTGTGGCAGGTGGTGTCATCATGGCGGGGAGCCTGAAGATTGGGCGTT 991 0 701 .......................................................................................... 790 2 446 .......................................................................................... 357 Y C M I G G A S V I N G H M E I C D K V T V T G M G M V M R Query_1 992 ACTGTATGATTGGTGGAGCAAGTGTTATCAATGGTCACATGGAAATTTGTGATAAAGTTACGGTAACAGGAATGGGGATGGTTATGCGTC 1081 0 791 .......................................................................................... 880 2 356 .......................................................................................... 267 P I T E P G V Y S S G I P L Q P N K A W R K T A A L V L R I Query_1 1082 CTATCACTGAACCTGGGGTTTATTCTTCTGGCATTCCGTTACAACCGAATAAAGCATGGCGTAAAACCGCAGCACTGGTGCTGCGAATTG 1171 0 881 .......................................................................................... 970 2 266 .......................................................................................... 177 D E M Q K R L K S L E R Q V E N S D K * H W K W I T D P N S Query_1 1172 ATGAAATGCAAAAAAGGCTAAAGTCGCTAGAACGTCAAGTAGAAAATAGCGACAAGTAACATTGG-AAGTGGATAACGGATCCGAATTCG 1260 0 971 ........................................................ 1026 2 176 .................................................................A........................ 87 S S V D K L A A A L E H H H H H H * D P A A Query_1 1261 AGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccacc-acTGAGATCCGGCTGCTA 1327 2 86 ..................................N..............A.................. 19 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4832225 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 13, 2018 11:15 AM Number of letters in database: 3,463 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95896.102 (IDP95896.1-240.F..pMCSG68)
MFDMRLSHERGAAHHGWLQSKHTFSFANYWDPKQVGFSDLLVINDDTVAPSKGFGTHPHRNMEIISYVLEGALEHKDSMGTGSVIVPGDIQLMSAGRGVAHSEFNHSAQENVHFLQIWVVPNEVNTTPGYQQLHISEEDKRGKLHLTISPKGGEKTLSIKQDINIYSGLFDGEETAEFAIPEGRYVYLHVAKGRIDVNGKTFNTGDAARIRDGGKLSFTNGDNAEILIFDMRPEEIPTMP
ATGTTTGATATGCGTTTATCTCATGAGCGTGGTGCAGCACATCATGGCTGGTTACAGTCAAAACACACGTTTTCATTTGCAAATTATTGGGACCCGAAACAAGTTGGCTTTTCAGATTTACTTGTAATTAATGACGATACAGTTGCTCCATCAAAAGGCTTTGGTACTCACCCTCACCGTAACATGGAAATTATTTCTTATGTGCTTGAAGGTGCGTTAGAACATAAAGACTCAATGGGTACAGGTTCGGTTATTGTGCCTGGTGATATTCAGTTAATGAGCGCAGGCCGTGGGGTTGCACATAGTGAATTTAATCACTCTGCTCAAGAAAACGTGCACTTCTTGCAAATTTGGGTAGTTCCAAATGAAGTGAACACAACACCGGGCTATCAACAATTGCATATTTCCGAGGAAGACAAACGCGGCAAATTGCACTTGACGATTTCACCAAAAGGTGGCGAAAAAACTTTATCGATCAAACAAGACATTAATATTTATTCAGGTCTTTTTGATGGTGAAGAAACAGCAGAGTTTGCAATTCCTGAAGGTCGTTATGTTTACCTACATGTGGCAAAAGGCCGTATTGATGTAAACGGTAAAACCTTTAATACAGGTGATGCTGCGCGTATTCGCGATGGAGGCAAGTTATCATTTACCAATGGTGACAATGCTGAAATTCTGATTTTTGATATGCGTCCGGAAGAAATTCCAACCATGCCA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,557 total letters Query= IDP95896.102 Length=1125 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1299 0.0 2_10D_D2_IDP95896.102_T7term.seq 1274 0.0 1_06D_D2_IDP95896.102_T7S.seq 1225 0.0 R N N F V * L * E G D I H M H H H H H H S S G V D L W S H P Query_1 67 AGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCG 156 0 198 ........... 188 2 921 ...N... 915 2 643 ............ 654 1 19 ....................................................................................... 105 1 349 ........... 339 Q F E K G T E N L Y F Q S N A M F D M R L S H E Query_1 157 C-AG-TTCG--AAAAGGGTACC--GAGAA-CCTGTAC--TTCCAA-TCCAATGCCATGTTTGA--TATGCGTTT--ATCTC-ATG-AGCG 230 0 1 ........--.........--.....-...-.... 29 0 682 ..T.....--....... 696 2 914 .C..-....GA.........A.CG.....A......ACT..N...N.AN..N...N......GAT.T....G..TA.....N...N.... 826 2 158 ..T.....--....... 144 1 106 .-..N....--...........--.....-.......--......-.................--.........--.....-...-.... 180 R G A A H H G W L Q S K H T F S F A N Y W D P K Q V G Query_1 231 T-GGTGCA-GCACATC-ATGGCTGGTTAC-AGTCAAAACA-CACGTTTT-CATTT-GCAAATTATTGGG-ACCCGAA-ACAAG-TTGGCT 310 0 30 .-......-.......-............-..........-........-.....-.............-.......-.....-...... 109 2 825 .T......AN......C............C........N.N........T.....T........A....G...N...G.....N...... 736 1 181 .-......-.......-............-..........-........-.....-.............-.......-.....-...... 260 F S D L L V I N D D T V A P S K G F G T H P H R N M E I I Query_1 311 TTTCA-GATTTACTTGTAA-TTAATGACGATACAGTTGCTCCATCAAAAGGCTTTGG-TACTCACCCTCACCGTAACATGGAAA-TTATT 396 0 110 .....-.............-.....................................-..........................-..... 195 2 735 .....A....A...N..N.N.........C.................N...NN....G..........................A..... 646 1 261 .....-.............-.....................................-..........................-..... 346 S Y V L E G A L E H K D S M G T G S V I V P G D I Q L M S Query_1 397 TCTT-ATGTGCTTGAAGGTGCGTTAGAACATAAAGACTCAATGGGTACAGGTTCGGTTATTGTGCCTGGTGATATTCAGTTAATGAGCGC 485 0 196 ....-..................................................................................... 284 2 645 ....T..........................................................N.......................... 556 1 347 ....-..................................................................................... 435 A G R G V A H S E F N H S A Q E N V H F L Q I W V V P N E V Query_1 486 AGGCCGTGGGGTTGCACATAGTGAATTTAATCACTC-TGCTCAAGAAAACGTGCACTTCTTGCAAATTTGGGTAGTTCCAAATGAAGTGA 574 0 285 ....................................-..................................................... 373 2 555 ....................................-..................................................... 467 1 436 ....................................N..................................................... 525 N T T P G Y Q Q L H I S E E D K R G K L H L T I S P K G G E Query_1 575 ACACAACACCGGGCTATCAACAATTGCATATTTCCGAGGAAGACAAACG-CGGCAAATTGCACTTGACGATTTCACCAAAAGGTGGCGAA 663 0 374 .................................................-........................................ 462 2 466 .................................................-........................................ 378 1 526 .................................................N........................................ 615 K T L S I K Q D I N I Y S G L F D G E E T A E F A I P E G Query_1 664 AAAAC-TTTATCGATCAAACAAGACATTAATATTTATTCAGGTCTTTTTGATGGTGAAGAAACAGCAGAGTTTGCAATTCCTGAAGGTCG 752 0 463 .....-.................................................................................... 551 2 377 .....-......................N............................................................. 289 1 616 .....T............N.......................................N..N....GA.T...............N.... 705 R Y V Y L H V A K G R I D V N G K T F N T G D A A R I R Query_1 753 TTATGTTTACCTA-CATGTGGCAAAAGG--CCGT--ATTGATGTAAAC--GGTAAAA-CCTTTAATACAGGTGATGCTGCGCGTATTCGC 834 0 552 .............-..............--....--............--.......-................................ 633 2 288 .............-..............--..C.--............--.......-................................ 207 1 706 ......C.N..N.C.N......N.....GC....TN.......N....CN.CC....C.............. 777 D G G K L S F T N G D N A E I L I F D M R P E E I P T M P * Query_1 835 GATGGAGGCAAGTTATCATTTACCAATGGTGACAATGCTGAAATTCTGATTTTTGATATGCGTCCGGAAGAAATTCCAACCATGCCATAA 924 0 634 ....................................................................................... 720 0 1 ..G............ 15 2 206 .......................................................................................... 117 1 152 ..G............ 166 H W K W I T D P N S S S V D K L A A A L E H H H H H H * D Query_1 925 -CATTGGAA-GTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGAT- 1011 2 116 G........T.....................................................................--GN......G 29 P A A N K A R Query_1 1012 CCGGCTGCTAACAAAGCCCGAA 1033 2 28 ..........N.-......N.. 8 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3891481 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 13, 2018 11:15 AM Number of letters in database: 3,557 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96162.102 (IDP96162.1-500.F..pMCSG68)
MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLAESLSEIGVPVFMADVKGDLTGVAQAGTVSEKLLARLKNIGVNDWQPHANPVVVWDIFGEKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQYIGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKGVINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAPQVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAVKAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTEDERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILGGLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR
ATGAGTGAACCCCTGTTAATTGCCCGCACGCCGGACACAGAACTGTTTTTACTGCCGGGAATGGCTAACCGTCATGGGCTGATTACCGGCGCAACGGGGACGGGTAAAACCGTTACGCTGCAAAAACTGGCGGAGTCATTGTCGGAAATCGGCGTGCCGGTGTTTATGGCTGATGTGAAAGGCGATCTGACCGGCGTCGCGCAGGCAGGAACGGTGTCGGAAAAACTGCTCGCAAGGCTTAAAAATATCGGCGTCAATGACTGGCAACCGCATGCCAATCCGGTGGTGGTGTGGGATATCTTTGGCGAGAAAGGCCATCCGGTGCGGGCGACGGTTTCGGATCTGGGGCCGCTGTTGCTGGCACGACTGTTGAATCTCAACGATGTGCAATCTGGCGTGCTGAATATCATCTTCCGCATTGCTGACGATCAGGGGCTGTTACTGCTCGACTTTAAAGATCTGCGGGCAATTACCCAGTACATCGGCGATAACGCCAAATCCTTCCAGAATCAGTACGGTAATATCAGTAGCGCATCGGTTGGTGCCATCCAGCGCGGGCTGTTGTCGCTGGAACAGCAAGGCGCAGCACACTTCTTTGGTGAGCCGATGCTGGATATCAAAGACTGGATGCGCACCGATGCCAACGGTAAAGGCGTTATCAATATCCTCAGCGCCGAGAAACTTTATCAGATGCCGAAACTGTACGCCGCCAGCCTGCTGTGGATGCTTTCAGAGTTGTATGAACAATTGCCGGAAGCGGGCGATCTGGAGAAACCAAAACTGGTGTTTTTCTTCGACGAGGCACATCTGCTGTTTAACGATGCCCCGCAGGTACTGCTGGATAAGATTGAGCAGGTGATAAGGCTTATTCGCTCAAAAGGCGTGGGCGTCTGGTTCGTTTCGCAAAACCCGTCTGATATTCCGGATAATGTGCTCGGGCAGCTCGGTAATCGCGTTCAACATGCTTTGCGGGCTTTTACGCCAAAAGATCAGAAAGCAGTGAAGGCAGCGGCGCAAACCATGCGGGCCAATCCGGCGTTTGATACCGAAAAGGCGATTCAGGAACTGGGCACCGGCGAGGCGTTGATCTCTTTTCTGGATGCGAAAGGAAGCCCTTCTGTGGTGGAGCGTGCGATGGTGATCGCGCCTTGTTCGCGGATGGGGCCGGTGACGGAAGATGAGCGTAATGGCTTGATTAATCACTCTCCGGTGTATGGCAAATATGAGGATGAGGTGGACCGGGAATCCGCCTATGAGATGTTGCAAAAAGGCTTTCAGGCCAGTACCGAGCAGCAAAATAATCCTCCCGCGAAAGGGAAAGAGGTAGCGGTGGATGACGGCATTCTTGGTGGATTGAAGGATATTTTGTTCGGCACTACCGGACCACGCGGCGGGAAGAAAGATGGTGTGGTGCAAACAATGGCCAAAAGCGCCGCTCGCCAAGTGACGAATCAGATTGTACGCGGGATGTTGGGGAGTTTGCTGGGGGGGAGAAGAAGG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,713 total letters Query= IDP96162.102 Length=1905 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2706 0.0 2_10B_B2_IDP96162.102_T7term.seq 1784 0.0 1_06B_B2_IDP96162.102_T7S.seq 1714 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 58 TTCCCC-TCTAGAAATAATTTT-GTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGT 145 1 4 ......N...NA..........T................................................................... 93 W S H P Q F E K G T E N L Y F Q S N A M S E P L L I A R T P Query_1 146 GGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGAGTGAACCCCTGTTAATTGCCCGCACGCCGG 235 0 1 .................................. 34 0 472 ............ 461 1 94 .......................................................................................... 183 1 621 ............ 610 D T E L F L L P G M A N R H G L I T G A T G T G K T V T L Q Query_1 236 ACACAGAACTGTTTTTACTGCCGGGAATGGCTAACCGTCATGGGCTGATTACCGGCGCAACGGGGACGGGTAAAACCGTTACGCTGCAAA 325 0 35 .......................................................................................... 124 1 184 .......................................................................................... 273 K L A E S L S E I G V P V F M A D V K G D L T G V A Q A G T Query_1 326 AACTGGCGGAGTCATTGTCGGAAATCGGCGTGCCGGTGTTTATGGCTGATGTGAAAGGCGATCTGACCGGCGTCGCGCAGGCAGGAACGG 415 0 125 .......................................................................................... 214 1 274 .......................................................................................... 363 V S E K L L A R L K N I G V N D W Q P H A N P V V V W D I F Query_1 416 TGTCGGAAAAACTGCTCGCAAGGCTTAAAAATATCGGCGTCAATGACTGGCAACCGCATGCCAATCCGGTGGTGGTGTGGGATATCTTTG 505 0 215 .......................................................................................... 304 1 364 .......................................................................................... 453 G E K G H P V R A T V S D L G P L L L A R L L N L N D V Q S Query_1 506 GCGAGAAAGGCCATCCGGTGCGGGCGACGGTTTCGGATCTGGGGCCGCTGTTGCTGGCACGACTGTTGAATCTCAACGATGTGCAATCTG 595 0 305 .......................................................................................... 394 1 454 .......................................................................................... 543 G V L N I I F R I A D D Q G L L L L D F K D L R A I T Q Y I Query_1 596 GCGTGCTGAATATCATCTTCCGCATTGCTGACGATCAGGGGCTGTTACTGCTCGACTTTAAAGATCTGCGGGCAATTACCCAGTACATCG 685 0 395 .......................................................................................... 484 0 28 ............ 17 2 1166 .....N..NN..ANTG...... 1145 1 544 .......................................................................................... 633 1 177 ............ 166 G D N A K S F Q N Q Y G N I S S A S V G A I Q R G L L S L Query_1 686 GCGATAACGCCAAATCC--TTCCA-GAATCAGTACGGTAATATCAGTAGCGCATCGGTTGGTGCCATCC-AGCGCGGGCTGTTGTCGCTG 771 0 485 .................--.....-............................................-.................... 570 2 1144 ..AGN..........A.CA.A...N.....N..N..NN.-....C.........NN-.....N......CNNNN................ 1057 1 634 .................--.....-............................................-.................... 719 E Q Q G A A H F F G E P M L D I K D W M R T D A N G K G V Query_1 772 GAACAGCAAGGCGCAGCACAC-TTCTTTGGTGAGCCGATGCTGGATATCAAAGACTGGATGCGCACCGATGCCAACGGTAAAGGCGTT-A 859 0 571 .....................-..................................................................-. 658 2 1056 N.-..................-.....-........-........NT....-......................-......GA.....N. 973 1 720 .....................T...................N...............................C....N.........-. 808 I N I L S A E K L Y Q M P K L Y A A S L L W M L S E L Y E Q Query_1 860 TCAATATCCTCAGCGCCGAGAAACTTTATCAGATGCCGAAACTGTACGCCGCCAGCCTGCTGTGGATGCTTTCAGAGTTGTATGAACAAT 949 0 659 .......................................................................................... 748 2 972 ..........................................N......N-.........N..........CN.........NN.-.... 885 1 809 ..-..........................................N..N..N...N.................................. 897 L P E A G D L E K P K L V F F F D E A H L L F N D A P Q V L Query_1 950 TGCCGGAAGCGGGCGATCTGGAGAAACCAAAACTGGTGTTTTTCTTCGACGAGGCACATCTGCTGTTTAACGATGCCCCGCAGGTAC-TG 1038 0 749 .......................................................................................-.. 837 2 884 ...GN........................................-.......................-.................-.. 798 1 898 .................N...............................................-..N.....N...GT..-....N.. 985 L D K I E Q V I R L I R S K G V G V W F V S Q N P S D I P Query_1 1039 CTGGATAAGATTGAGCAGGTGATAAGGCTTATT-CGCTCAAAAGGCGTGGGCGTCTGGTTCGTTTCGCAAAA-CCCGTCTGATATTCCGG 1126 0 838 .................................-......................................-................. 925 2 797 .................................-......................................A................. 709 1 986 ......-.......N..........T.......C.N...... 1026 D N V L G Q L G N R V Q H A L R A F T P K D Q K A V K A A A Query_1 1127 -ATAATGTGCTCGGGCAGCTCGGTAATCGCGTTCAACATGCTTTGCGGGCTTTTACGCCAAAAGATCAGAAAGCAGTGAAGGCAGCGGCG 1215 0 926 -......................................................................................... 1014 2 708 G......................................................................................... 619 Q T M R A N P A F D T E K A I Q E L G T G E A L I S F L D A Query_1 1216 CAAACCATGCGGGCCAATCCGGCGTTTGATACCGAAAAGGCGATTCAGGAACTGGGCACCGGCGAGGCGTTGATCTCTTTTCTGGATGCG 1305 0 1015 .......................................................................................... 1104 2 618 ......................................................................................N... 529 K G S P S V V E R A M V I A P C S R M G P V T E D E R N G L Query_1 1306 AAAGGAAGCCCTTCTGTGGTGGAGCGTGCGATGGTGATCGCGCCTTGTTCGCGGATGGGGCCGGTGACGGAAGATGAGCGTAATGGCTTG 1395 0 1105 .......................................................................................... 1194 2 528 .......................................................................................... 439 I N H S P V Y G K Y E D E V D R E S A Y E M L Q K G F Q A S Query_1 1396 ATTAATCACTCTCCGGTGTATGGCAAATATGAGGATGAGGTGGACCGGGAATCCGCCTATGAGATGTTGCAAAAAGGCTTTCAGGCCAGT 1485 0 1195 .......................................................................................... 1284 2 438 .......................................................................................... 349 T E Q Q N N P P A K G K E V A V D D G I L G G L K D I L F G Query_1 1486 ACCGAGCAGCAAAATAATCCTCCCGCGAAAGGGAAAGAGGTAGCGGTGGATGACGGCATTCTTGGTGGATTGAAGGATATTTTGTTCGGC 1575 0 1285 .......................................................................................... 1374 2 348 .......................................................................................... 259 T T G P R G G K K D G V V Q T M A K S A A R Q V T N Q I V R Query_1 1576 ACTACCGGACCACGCGGCGGGAAGAAAGATGGTGTGGTGCAAACAATGGCCAAAAGCGCCGCTCGCCAAGTGACGAATCAGATTGTACGC 1665 0 1375 .......................................................................................... 1464 2 258 .......................................................................................... 169 G M L G S L L G G R R R * H W K W I T D P N S S S V D K L A Query_1 1666 GGGATGTTGGGGAGTTTGCTgggggggAGAAGAAGGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCG 1755 0 1465 .................................... 1500 2 168 ...................................................................................N..N... 79 A A L E H H H H H H * D P A A N K A R K E Query_1 1756 GCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCG-AAAGGAAGC 1820 0 1103 ..-......... 1113 2 78 ...................................................N....A....... 15 2 530 ..-......... 520 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 6913856 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 13, 2018 11:15 AM Number of letters in database: 3,713 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96867.102 (IDP96867.1-1332.F..pMCSG68)
MTENNKLAVSPNAWFAIDRGQSKNPTLALHTVQLKSEQALKHGIADSDWVVVFDTTGHITRIGRILRIRSDLETTTLCFDRILQVEPLIPVGMTSLTLPAKGSFGRIQWKEFIEMLPNTLNTSIAEIPTIEDQTYIRELLQLAVMDDLLGPAAGPNELIVDMGVRDRYLVGKLAPREAAERSSEFPVDPENADDDVGDQIVKSQTTKVHSPKVSGRGEPDVPEEIDAASNQSLVPSSLGMTFCVDGDIDQIELEVRWGRYERSNDHEIYRIRKNKETGVEEQTKVKAWQRFPSGGKITLSLVEGAISPQSLDSSSPEVLIQGTIRPKNENGDRLVTIFLVNTQKEPETNRDAAWVFQPEIIARPVKDAVERSIFRRKPVLDCDGMDPEREALEMIYRNHVEFAVGHGVAVHAEPADNTELATEIRTTVMPQYEVQRTETPGLDPSDRPAMQEMVKSGLLDMQKLATLEVEPLIDALNVLTKDYLDWISEQRASVGIKITGFETQSQIAMDRCKEIHSRLQKGIDTLKLNEKALAAFRFANKAMATQRVRSLYALAKRRGEDTTIESFDIEKNRSWRPFQLAFLLLSIPSLADPNHSDRVQPVNAYADLLWFPTGGGKTEAYLGVAAFTMAIRRMQGNLGGYDSSRGLAVIMRYTLRLLTLQQFQRATALICAMEVLRREALNNGDVSLGLEPFTIGLWVGNKVTPGSTEESHRAIEDARNPGKNHAGTASPAQLTSCPWCGSSIIPGQDVEVKKDKLGGRTFVFCGDKKGRCDFSKGKSSKQAHPGLPVLVVDEEIYHRPPTMMIATVDKFAMMAWRGQVRTLFGRVGLECERHGLLWQGASCTGNHPAAKGQPSTKVRVINPIRPPDLIIQDEFHLISGPLGTMVGLYETAVDELCSWNLDGKSVRPKIIASTATVRKAKEQVNNVFMRQVAVFPPHGIDVEDNFFSVQRPIKEKYGRRYLGVCSPGSSRPAMLIRVYTAFLTAAQELFNHFGEPADPYMTMVGYFNSLRELGGMKRLAEDDVQTRSYRVRMSMVERPALAQRSISNVRELTSRVSSQDIPKYLDNLEVKFKSEFDTTTGKYVTKWQEGDTRAIDIVLATNMLSVGVDVNRLGLMAVNGQPKGTAEYIQATSRVGRSFPGLVCTVLTWARPRDLSHYETFEHYHATFYKHVEAQSVTPFSPRAMDRGLTGALLSLVRLENDEFSPNDGAGKLNMSNQAEISNAIKALANRAGNVAEDNSRKKLAENELKERADEWAKKASMGGRILAYEKRGPEKDKTVSLLDSPGLKPWDNWTVPMSMREVEPGVRLIMNTNHIADDHDWKPRPATKDED
ATGACAGAGAACAATAAGCTAGCTGTATCACCTAATGCATGGTTCGCTATTGATCGTGGCCAATCTAAAAATCCTACTTTGGCCCTTCATACAGTACAGCTAAAAAGTGAACAAGCTCTCAAGCACGGTATTGCTGATAGCGATTGGGTCGTCGTCTTTGACACTACAGGTCATATCACGCGAATCGGACGTATTCTGCGCATTCGCTCTGATCTCGAAACTACCACCTTATGTTTCGACCGAATTTTGCAGGTTGAACCACTCATTCCCGTTGGCATGACTTCACTTACTCTGCCTGCAAAAGGAAGCTTTGGCAGAATTCAGTGGAAAGAATTCATTGAAATGCTTCCAAATACACTAAATACAAGCATTGCTGAAATACCAACGATCGAAGATCAAACTTACATTCGCGAATTACTGCAATTAGCAGTTATGGATGATTTACTCGGGCCGGCAGCTGGCCCTAATGAGCTTATCGTCGATATGGGCGTAAGGGATCGCTATCTTGTTGGTAAGCTTGCTCCACGTGAAGCAGCCGAGCGCAGCTCTGAGTTTCCAGTGGATCCAGAGAATGCTGATGATGATGTTGGTGACCAAATAGTTAAGTCTCAGACAACAAAAGTGCATTCTCCAAAAGTGTCTGGCCGAGGAGAACCCGATGTCCCGGAAGAAATTGATGCCGCCAGCAACCAATCATTGGTACCAAGTAGTCTGGGAATGACTTTTTGTGTCGATGGCGATATTGATCAAATAGAACTAGAAGTTCGTTGGGGACGTTATGAGCGTAGCAATGACCATGAGATCTATCGAATCCGCAAGAACAAGGAGACTGGGGTCGAAGAACAGACCAAAGTCAAAGCTTGGCAGCGTTTTCCTAGTGGCGGGAAGATAACATTATCACTAGTAGAGGGGGCTATTTCCCCTCAATCTTTAGATAGTTCATCTCCTGAAGTACTAATACAGGGCACCATTCGTCCCAAGAATGAAAATGGTGATCGTCTGGTCACTATATTTCTTGTAAATACTCAGAAAGAGCCAGAAACCAACCGAGATGCAGCATGGGTATTCCAACCAGAAATCATTGCTCGCCCAGTGAAGGACGCAGTCGAAAGATCTATTTTTAGGCGCAAGCCAGTTTTGGATTGTGACGGTATGGATCCTGAACGCGAAGCGCTGGAAATGATTTACCGTAACCATGTTGAATTTGCAGTCGGGCATGGTGTTGCAGTACATGCTGAACCTGCAGATAACACTGAACTTGCGACTGAAATTCGTACAACTGTCATGCCACAGTATGAAGTTCAGAGAACAGAGACCCCCGGCCTTGATCCATCAGATCGTCCGGCGATGCAAGAAATGGTCAAAAGTGGTTTACTTGACATGCAGAAACTGGCCACTTTGGAGGTGGAACCACTGATCGATGCATTGAATGTACTAACCAAGGACTATCTGGATTGGATTAGTGAACAACGTGCCAGTGTAGGTATTAAAATTACAGGTTTTGAAACCCAATCTCAGATCGCAATGGATCGTTGTAAGGAGATCCATTCTCGCCTGCAAAAGGGTATTGATACACTTAAGCTTAATGAAAAAGCGCTGGCAGCATTTCGTTTCGCCAACAAAGCAATGGCAACGCAGCGAGTACGAAGTTTATACGCGTTGGCTAAACGCCGTGGTGAAGATACGACTATCGAATCATTTGATATTGAAAAGAATCGTAGCTGGCGTCCTTTTCAGTTGGCATTCTTGTTGCTCTCTATACCTTCGTTGGCAGACCCAAACCATTCCGATCGTGTGCAGCCTGTAAATGCATATGCTGATCTTTTATGGTTCCCCACGGGTGGTGGTAAAACTGAAGCTTATTTAGGCGTTGCGGCTTTTACAATGGCCATACGCCGCATGCAGGGTAATTTGGGGGGGTATGATAGTTCGCGCGGTTTGGCGGTAATAATGCGATATACATTGCGCCTGCTAACATTACAACAATTCCAGCGTGCGACAGCCTTAATTTGTGCTATGGAAGTTTTGCGGCGAGAAGCACTCAATAATGGCGACGTGTCTCTCGGATTAGAACCATTTACTATTGGTCTTTGGGTTGGCAACAAAGTAACGCCTGGTTCGACTGAAGAGAGTCATCGAGCTATTGAAGATGCCCGTAATCCGGGAAAAAACCATGCGGGAACGGCCTCACCTGCACAATTAACTAGTTGTCCATGGTGTGGTTCATCAATTATCCCCGGACAGGATGTTGAAGTTAAAAAGGATAAATTGGGGGGAAGGACGTTTGTTTTTTGCGGTGATAAAAAGGGGCGTTGTGACTTTTCCAAAGGAAAATCAAGTAAACAAGCCCATCCAGGATTGCCTGTATTAGTAGTGGATGAAGAGATCTATCATCGCCCTCCCACAATGATGATTGCTACTGTAGATAAGTTTGCCATGATGGCATGGCGTGGCCAGGTGAGAACGCTTTTCGGCCGTGTAGGATTGGAATGTGAACGACATGGTTTGCTCTGGCAGGGGGCTAGCTGCACAGGAAATCATCCCGCAGCCAAAGGACAACCTTCAACTAAAGTTAGGGTTATCAATCCAATACGACCACCGGACCTTATCATTCAGGATGAATTCCACTTAATTAGTGGACCTTTGGGCACTATGGTTGGATTGTATGAGACTGCTGTTGATGAATTATGTAGTTGGAATCTTGACGGGAAGTCGGTCAGACCAAAGATCATTGCTTCAACTGCAACAGTCCGTAAAGCTAAGGAACAGGTAAATAATGTTTTTATGCGCCAAGTGGCAGTATTTCCACCACATGGGATAGACGTTGAGGATAACTTTTTCTCAGTACAACGACCTATAAAAGAAAAGTATGGACGTCGTTATCTTGGGGTATGCTCGCCTGGCAGTTCAAGGCCCGCAATGCTGATCCGTGTTTACACTGCATTCCTGACCGCAGCACAGGAATTATTCAACCACTTTGGTGAGCCTGCTGACCCTTATATGACAATGGTTGGCTATTTCAACTCGCTACGCGAATTAGGCGGGATGAAGCGACTTGCAGAGGATGACGTACAAACACGTTCTTACCGGGTGCGAATGAGCATGGTGGAAAGACCGGCATTGGCACAACGTAGTATAAGTAATGTTCGTGAATTAACTTCCCGAGTATCCAGTCAGGATATCCCTAAATATCTTGATAATTTAGAGGTTAAGTTTAAATCTGAATTTGATACTACTACTGGTAAGTATGTGACTAAATGGCAGGAGGGGGATACTCGGGCTATAGATATCGTCCTTGCTACTAATATGTTGTCTGTCGGCGTCGATGTTAACCGACTTGGTCTTATGGCAGTAAATGGTCAGCCTAAAGGAACGGCCGAATATATCCAGGCTACCAGTCGTGTAGGGCGTTCATTTCCTGGTTTAGTTTGTACCGTACTAACTTGGGCTCGTCCAAGAGACCTTTCTCATTACGAAACATTCGAACATTACCATGCCACCTTCTACAAGCATGTAGAGGCCCAATCTGTTACTCCTTTTTCTCCTCGAGCGATGGACCGTGGTTTAACAGGTGCGTTATTAAGTCTTGTTCGTCTGGAAAACGACGAATTCAGTCCTAACGATGGGGCTGGTAAGTTGAATATGTCTAATCAAGCCGAGATCAGTAATGCGATTAAAGCACTGGCGAATCGCGCTGGCAATGTTGCAGAAGATAATTCTCGCAAAAAATTGGCAGAGAATGAACTGAAAGAACGTGCCGACGAATGGGCAAAAAAAGCGTCAATGGGCGGACGGATTCTGGCATATGAAAAGCGCGGGCCTGAAAAAGATAAGACTGTCAGCTTATTAGACTCTCCAGGCCTGAAGCCTTGGGACAACTGGACAGTGCCTATGTCAATGCGAGAAGTTGAACCAGGTGTGAGACTTATAATGAACACCAACCATATAGCGGACGATCACGATTGGAAGCCTCGTCCCGCAACTAAGGATGAGGAC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 6,302 total letters Query= IDP96867.102 Length=4401 Score E Sequences producing significant alignments: (Bits) Value 0_wt 7207 0.0 2_10A_A2_IDP96867.102_T7term.seq 1770 0.0 1_06A_A2_IDP96867.102_T7S.seq 1665 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 60 CCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTC 149 0 589 ..A...........G.....-..... 565 0 592 .....A........... 576 1 7 ........N...............-................................................................. 95 1 738 ..A...........G....TC..... 713 1 741 .....A........... 725 S H P Q F E K G T E N L Y F Q S N A M T E N N K L A V S P N Query_1 150 TCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGACAGAGAACAATAAGCTAGCTGTATCACCTAATGC 239 0 1 ...................................... 38 1 96 .......................................................................................... 185 A W F A I D R G Q S K N P T L A L H T V Q L K S E Q A L K H Query_1 240 ATGGTTCGCTATTGATCGTGGCCAATCTAAAAATCCTACTTTGGCCCTTCATACAGTACAGCTAAAAAGTGAACAAGCTCTCAAGCACGG 329 0 39 .......................................................................................... 128 1 186 .......................................................................................... 275 G I A D S D W V V V F D T T G H I T R I G R I L R I R S D L Query_1 330 TATTGCTGATAGCGATTGGGTCGTCGTCTTTGACACTACAGGTCATATCACGCGAATCGGACGTATTCTGCGCATTCGCTCTGATCTCGA 419 0 129 .......................................................................................... 218 1 276 ..................................................T....................................... 365 E T T T L C F D R I L Q V E P L I P V G M T S L T L P A K G Query_1 420 AACTACCACCTTATGTTTCGACCGAATTTTGCAGGTTGAACCACTCATTCCCGTTGGCATGACTTCACTTACTCTGCCTGCAAAAGGAAG 509 0 219 .......................................................................................... 308 0 1553 ............ 1564 1 366 .......................................................................................... 455 S F G R I Q W K E F I E M L P N T L N T S I A E I P T I E D Query_1 510 CTTTGGCAGAATTCAGTGGAAAGAATTCATTGAAATGCTTCCAAATACACTAAATACAAGCATTGCTGAAATACCAACGATCGAAGATCA 599 0 309 .......................................................................................... 398 1 456 .......................................................................................... 545 Q T Y I R E L L Q L A V M D D L L G P A A G P N E L I V D M Query_1 600 AACTTACATTCGCGAATTACTGCAATTAGCAGTTATGGATGATTTACTCGGGCCGGCAGCTGGCCCTAATGAGCTTATCGTCGATATGGG 689 0 399 .......................................................................................... 488 1 546 ...............................N.......................................................... 635 G V R D R Y L V G K L A P R E A A E R S S E F P V D P E N Query_1 690 CGTAAGGGATCGCTATCTTGTTGGTAAGCTTGCTCCACGTGAAGCAGCCGAGCGCAGCTCTGAG-TTTCCAGTGGATCCAGA-G-AATGC 776 0 489 ................................................................-.................-.-..... 575 1 636 ..............................................N.........N.......T.................-.A..... 724 1 80 .....A.-....A 69 A D D D V G D Q I V K S Q T T K V H S P K V S G R G E P D V Query_1 777 TGATGATGATGTTGGTGACCAAATAGTTAAGTCTCAGACAACAAAAGTGCATTCTCCAAAAGTGTCTGG-CCGAGGAGAACCCGATG-TC 864 0 576 .....................................................................-.................-.. 663 1 725 .........................................................-...........N.................T.. 813 1 68 ...........G.. 55 1 71 ...........A..... 55 P E E I D A A S N Q S L V P S S L G M T F C V D G D I D Query_1 865 CCGGAAGAAATTGATGCCGCCAGCAACCAATCA-TTGGTACCAAGTAGT-CTGGGAA-TGAC-TTTTTGTGTCGATGGCGATATTGATCA 950 0 664 .................................-...............-.......-....-........................... 749 1 814 ..N..........................TN..T...N.........N.N.......T....T.........................N. 903 Q I E L E V R W G R Y E R S N D H E I Y R I R K N K E T G Query_1 951 AATAGAACTAGAAGTT-CGTTGGGGACGTTATGAGCGTAGCAATGACCATG-AGATCT-ATCGAATCCGCAAGAACAAGGAGACTGGGGT 1037 0 750 ................-..................................-......-............................... 836 1 904 .N..............C.T...........N.-.N....CA.T........A.N....NN..........-.....C......N...... 991 V E E Q T K V K A W Q R F P S G G K I T L S L V E G A I Query_1 1038 CGAAGAACAGACCAAAGTCAAAGCTTGGCAGCGTTTTCCTAGTGG-CGGG-AAGATAACA-TTATCACTAGTAGAGGGGG--CTATTTC- 1121 0 837 .............................................-....-.........-...................--.......- 920 1 992 .N..............N.....T......NA..............N....A..A......N..N......N.........GNTN.....N 1081 S P Q S L D S S S P E V L I Q G T I R P K N E N G D R L V T Query_1 1122 CCCTCAATCTTTAGATAGTTCATCTCCTGAAGTACTAATACAGGGCACCATTCGTCCCAAGAATGAAAATGGTGATCGTCTGGTCACTAT 1211 0 921 .......................................................................................... 1010 0 2373 ............ 2362 1 1082 .....NN... 1091 I F L V N T Q K E P E T N R D A A W V F Q P E I I A R P V K Query_1 1212 ATTTCTTGTAAATACTCAGAAAGAGCCAGAAACCAACCGAGATGCAGCATGGGTATTCCAACCAGAAATCATTGCTCGCCCAGTGAAGGA 1301 0 1011 .......................................................................................... 1100 D A V E R S I F R R K P V L D C D G M D P E R E A L E M I Y Query_1 1302 CGCAGTCGAAAGATCTATTTTTAGGCGCAAGCCAGTTTTGGATTGTGACGGTATGGATCCTGAACGCGAAGCGCTGGAAATGATTTACCG 1391 0 1101 .......................................................................................... 1190 R N H V E F A V G H G V A V H A E P A D N T E L A T E I R T Query_1 1392 TAACCATGTTGAATTTGCAGTCGGGCATGGTGTTGCAGTACATGCTGAACCTGCAGATAACACTGAACTTGCGACTGAAATTCGTACAAC 1481 0 1191 .......................................................................................... 1280 T V M P Q Y E V Q R T E T P G L D P S D R P A M Q E M V K S Query_1 1482 TGTCATGCCACAGTATGAAGTTCAGAGAACAGAGACCCCCGGCCTTGATCCATCAGATCGTCCGGCGATGCAAGAAATGGTCAAAAGTGG 1571 0 1281 .......................................................................................... 1370 G L L D M Q K L A T L E V E P L I D A L N V L T K D Y L D W Query_1 1572 TTTACTTGACATGCAGAAACTGGCCACTTTGGAGGTGGAACCACTGATCGATGCATTGAATGTACTAACCAAGGACTATCTGGATTGGAT 1661 0 1371 .......................................................................................... 1460 I S E Q R A S V G I K I T G F E T Q S Q I A M D R C K E I H Query_1 1662 TAGTGAACAACGTGCCAGTGTAGGTATTAAAATTACAGGTTTTGAAACCCAATCTCAGATCGCAATGGATCGTTGTAAGGAGATCCATTC 1751 0 1461 .......................................................................................... 1550 S R L Q K G I D T L K L N E K A L A A F R F A N K A M A T Q Query_1 1752 TCGCCTGCAAAAGGGTATTGATACACTTAAGCTTAATGAAAAAGCGCTGGCAGCATTTCGTTTCGCCAACAAAGCAATGGCAACGCAGCG 1841 0 1551 .......................................................................................... 1640 0 294 ............ 305 1 441 ............ 452 R V R S L Y A L A K R R G E D T T I E S F D I E K N R S W R Query_1 1842 AGTACGAAGTTTATACGCGTTGGCTAAACGCCGTGGTGAAGATACGACTATCGAATCATTTGATATTGAAAAGAATCGTAGCTGGCGTCC 1931 0 1641 .......................................................................................... 1730 P F Q L A F L L L S I P S L A D P N H S D R V Q P V N A Y A Query_1 1932 TTTTCAGTTGGCATTCTTGTTGCTCTCTATACCTTCGTTGGCAGACCCAAACCATTCCGATCGTGTGCAGCCTGTAAATGCATATGCTGA 2021 0 1731 .......................................................................................... 1820 D L L W F P T G G G K T E A Y L G V A A F T M A I R R M Q G Query_1 2022 TCTTTTATGGTTCCCCACGGGTGGTGGTAAAACTGAAGCTTATTTAGGCGTTGCGGCTTTTACAATGGCCATACGCCGCATGCAGGGTAA 2111 0 1821 .......................................................................................... 1910 N L G G Y D S S R G L A V I M R Y T L R L L T L Q Q F Q R A Query_1 2112 TTTgggggggTATGATAGTTCGCGCGGTTTGGCGGTAATAATGCGATATACATTGCGCCTGCTAACATTACAACAATTCCAGCGTGCGAC 2201 0 1911 .......................................................................................... 2000 T A L I C A M E V L R R E A L N N G D V S L G L E P F T I G Query_1 2202 AGCCTTAATTTGTGCTATGGAAGTTTTGCGGCGAGAAGCACTCAATAATGGCGACGTGTCTCTCGGATTAGAACCATTTACTATTGGTCT 2291 0 2001 .......................................................................................... 2090 L W V G N K V T P G S T E E S H R A I E D A R N P G K N H A Query_1 2292 TTGGGTTGGCAACAAAGTAACGCCTGGTTCGACTGAAGAGAGTCATCGAGCTATTGAAGATGCCCGTAATCCGGGAAAAAACCATGCGGG 2381 0 2091 .......................................................................................... 2180 G T A S P A Q L T S C P W C G S S I I P G Q D V E V K K D K Query_1 2382 AACGGCCTCACCTGCACAATTAACTAGTTGTCCATGGTGTGGTTCATCAATTATCCCCGGACAGGATGTTGAAGTTAAAAAGGATAAATT 2471 0 2181 .......................................................................................... 2270 L G G R T F V F C G D K K G R C D F S K G K S S K Q A H P G Query_1 2472 GGGGGGAAGGACGTTTGTTTTTTGCGGTGATAAAAAGGGGCGTTGTGACTTTTCCAAAGGAAAATCAAGTAAACAAGCCCATCCAGGATT 2561 0 2271 .......................................................................................... 2360 L P V L V V D E E I Y H R P P T M M I A T V D K F A M M A W Query_1 2562 GCCTGTATTAGTAGTGGATGAAGAGATCTATCATCGCCCTCCCACAATGATGATTGCTACTGTAGATAAGTTTGCCATGATGGCATGGCG 2651 0 2361 .......................................................................................... 2450 0 964 ............ 953 R G Q V R T L F G R V G L E C E R H G L L W Q G A S C T G N Query_1 2652 TGGCCAGGTGAGAACGCTTTTCGGCCGTGTAGGATTGGAATGTGAACGACATGGTTTGCTCTGGCAGGGGGCTAGCTGCACAGGAAATCA 2741 0 2451 .......................................................................................... 2540 H P A A K G Q P S T K V R V I N P I R P P D L I I Q D E F H Query_1 2742 TCCCGCAGCCAAAGGACAACCTTCAACTAAAGTTAGGGTTATCAATCCAATACGACCACCGGACCTTATCATTCAGGATGAATTCCACTT 2831 0 2541 .......................................................................................... 2630 L I S G P L G T M V G L Y E T A V D E L C S W N L D G K S V Query_1 2832 AATTAGTGGACCTTTGGGCACTATGGTTGGATTGTATGAGACTGCTGTTGATGAATTATGTAGTTGGAATCTTGACGGGAAGTCGGTCAG 2921 0 2631 .......................................................................................... 2720 R P K I I A S T A T V R K A K E Q V N N V F M R Q V A V F P Query_1 2922 ACCAAAGATCATTGCTTCAACTGCAACAGTCCGTAAAGCTAAGGAACAGGTAAATAATGTTTTTATGCGCCAAGTGGCAGTATTTCCACC 3011 0 2721 .......................................................................................... 2810 P H G I D V E D N F F S V Q R P I K E K Y G R R Y L G V C S Query_1 3012 ACATGGGATAGACGTTGAGGATAACTTTTTCTCAGTACAACGACCTATAAAAGAAAAGTATGGACGTCGTTATCTTGGGGTATGCTCGCC 3101 0 2811 .......................................................................................... 2900 P G S S R P A M L I R V Y T A F L T A A Q E L F N H F G Query_1 3102 TGGCAGTTCAAGGCCCGCAATGCTGATCCGTGTTTACACTGCATTCCTGACCGCAGCACAGGAA-TTATTC--AACC-ACTTT-GGTGA- 3185 0 2901 ................................................................-......--....-.....-.....- 2984 2 1203 ......A......CA....N.....T....NA 1172 E P A D P Y M T M V G Y F N S L R E L G G M K R L A Query_1 3186 -GCCTGCT-GACCCT-TATATGA-CAATGGTT--GGCTATTT-CAACTCGCTACGCGAATT-AGGCGGG-ATGAA-GCGACTT-GCAG-A 3263 0 2985 -.......-......-.......-........--........-..................-.......-.....-.......-....-. 3062 2 1171 G..N....N......A.......N......G.TG..N.....TN.................T.......G.....A..N....T....N. 1082 E D D V Q T R S Y R V R M S M V E R P A L A Q R S I S N Query_1 3264 GGATGACGTAC-AAACACGTTCTTA-CCGGG-TGCGAA-TGAGCATGGTGGAAAG--ACCGGCATTGGCA-CAACGTAGT-ATAAGTAAT 3345 0 3063 ...........-.............-.....-......-................--.............-.........-......... 3144 2 1081 ...N.......C..........C..A.....N..N...A...............AGA........G...NN........NN......... 992 V R E L T S R V S S Q D I P K Y L D N L E V K F K S E F Query_1 3346 GTT-CGTGAATTAACTTCCCG-AGT-ATCCAGTCAGGATATCCCTAAATATCTTGATAA-TTTAGA-GGTTAAGTTTAAATCTGAATTT- 3429 0 3145 ...-.................-...-.................................-......-......................- 3228 2 991 ...T...N.............N...T...........N.........N-..........A......N......................T 903 D T T T G K Y V T K W Q E G D T R A I D I V L A T N M L S Query_1 3430 GATACTACTACTGGTAAGTA-TGTGACTAAATGGCAGGAGGGGG-ATACTCGGGCTATAGATATCGTCCTTGCTACTAATATGTTGTCTG 3517 0 3229 ....................-.......................-............................................. 3316 2 902 ....................A.......................G............................................. 813 V G V D V N R L G L M A V N G Q P K G T A E Y I Q A T S R Query_1 3518 TCGGCGTCGATGTTAACCGACTTGGTCTT-ATGGCAGTAAATGGTCAGCCTAAAGGAACGGCCGAA-TATATCCAGGCTACCAGTCGTGT 3605 0 3317 .............................-....................................-....................... 3404 2 812 .............................T....................................A.........-............. 724 V G R S F P G L V C T V L T W A R P R D L S H Y E T F E H Y Query_1 3606 AGGGCGTTCATTTCCTGGTTTAGTTTGTACCGTACTAACTTGGGCTCGTCCAAGAGACCTTTCTCATTACGAAACATTCGAACATTACCA 3695 0 3405 .......................................................................................... 3494 2 723 .......................................................................................... 634 H A T F Y K H V E A Q S V T P F S P R A M D R G L T G A L L Query_1 3696 TGCCACCTTCTACAAGCATGTAGAGGCCCAATCTGTTACTCCTTTTTCTCCTCGAGCGATGGACCGTGGTTTAACAGGTGCGTTATTAAG 3785 0 3495 .......................................................................................... 3584 2 633 .......................................................................................... 544 S L V R L E N D E F S P N D G A G K L N M S N Q A E I S N A Query_1 3786 TCTTGTTCGTCTGGAAAACGACGAATTCAGTCCTAACGATGGGGCTGGTAAGTTGAATATGTCTAATCAAGCCGAGATCAGTAATGCGAT 3875 0 3585 .......................................................................................... 3674 2 543 .......................................................................................... 454 I K A L A N R A G N V A E D N S R K K L A E N E L K E R A D Query_1 3876 TAAAGCACTGGCGAATCGCGCTGGCAATGTTGCAGAAGATAATTCTCGCAAAAAATTGGCAGAGAATGAACTGAAAGAACGTGCCGACGA 3965 0 3675 .......................................................................................... 3764 2 453 .......................................................................................... 364 E W A K K A S M G G R I L A Y E K R G P E K D K T V S L L D Query_1 3966 ATGGGCaaaaaaaGCGTCAATGGGCGGACGGATTCTGGCATATGAAAAGCGCGGGCCTGAAAAAGATAAGACTGTCAGCTTATTAGACTC 4055 0 3765 .......................................................................................... 3854 2 363 .......................................................................................... 274 S P G L K P W D N W T V P M S M R E V E P G V R L I M N T N Query_1 4056 TCCAGGCCTGAAGCCTTGGGACAACTGGACAGTGCCTATGTCAATGCGAGAAGTTGAACCAGGTGTGAGACTTATAATGAACACCAACCA 4145 0 3855 .......................................................................................... 3944 2 273 .......................................................................................... 184 H I A D D H D W K P R P A T K D E D * H W K W I T D P N S S Query_1 4146 TATAGCGGACGATCACGATTGGAAGCCTCGTCCCGCAACTAAGGATGAGGACTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTC 4235 0 3945 .................................................... 3996 2 183 .......................................................................................... 94 S V D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 4236 CGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCT-AACAAAGCCCGAAAGGAA 4314 2 93 ............................---..............................A..............A... 17 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 27384984 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 13, 2018 11:15 AM Number of letters in database: 6,302 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96872.102 (IDP96872.1-352.F..pMCSG68)
MILVKSLDADGSPKEKRRKGTGKITLTEVAEFVGVSAMTVSRALRMPEKVNPELRDRIDAAVSQLGYVPNLQARNLASVHSDLILAVVPTFSSPGFLPVSEALQKVLTERGYTMMFMESGHDGQSEQKTFEKMLAYNPAAIIQFNIDTIESCTQLLANVDVPILEIGALNTHPVGMCIGVDYGKAVKQIVTHLADASLKNIALLCTPANNTMFRQLLSGWNTAMLALNRSPHRVVTTHLPSTIATGVNIFKDMMITWGDLDALICTSDEMACGCMMACHSAGIKVPNTVAIASLGGGVLSTVCSPALTTVEFPWHDIGVKAGKALLELLNDKPGEKFIEIPSVLKVRASTAA
GTGATTTTGGTGAAGAGTTTAGACGCGGACGGTAGCCCGAAAGAAAAACGACGTAAAGGAACGGGTAAGATAACCCTGACCGAGGTGGCTGAGTTTGTCGGTGTGAGCGCCATGACCGTCTCGCGAGCGCTGAGAATGCCTGAAAAAGTGAATCCTGAGCTTCGCGATCGCATCGATGCGGCCGTCAGTCAACTCGGCTATGTCCCTAATCTGCAGGCACGTAATCTGGCGTCGGTCCATTCCGATCTTATTCTCGCCGTCGTTCCCACCTTCTCTTCGCCGGGTTTTTTGCCGGTCTCTGAAGCGCTGCAAAAAGTGCTGACCGAACGCGGATATACCATGATGTTTATGGAATCCGGGCATGACGGTCAAAGTGAACAAAAGACGTTTGAAAAAATGCTCGCCTATAACCCGGCGGCCATTATTCAGTTCAATATCGATACCATTGAGAGCTGCACCCAGTTGCTGGCCAACGTGGATGTTCCTATTCTGGAAATTGGCGCGCTAAATACCCACCCTGTGGGTATGTGCATCGGCGTGGATTACGGTAAGGCCGTAAAACAGATCGTGACTCATCTGGCCGACGCCTCATTAAAAAACATCGCACTGCTCTGTACCCCGGCTAATAACACAATGTTTCGCCAGTTGCTGAGCGGTTGGAATACGGCGATGCTCGCTCTGAACCGCTCCCCGCACCGGGTGGTAACCACGCATTTGCCATCCACCATCGCCACAGGCGTCAATATATTCAAGGATATGATGATTACCTGGGGCGATCTGGATGCGTTAATTTGCACGTCGGATGAAATGGCCTGTGGATGTATGATGGCCTGCCACAGTGCCGGCATTAAGGTGCCCAATACCGTAGCCATTGCGAGTTTAGGCGGAGGAGTGTTATCGACAGTCTGTTCCCCTGCCCTGACAACCGTTGAGTTTCCCTGGCATGACATTGGGGTGAAAGCCGGTAAAGCTCTTCTCGAACTGCTTAACGATAAGCCTGGTGAAAAGTTCATTGAAATTCCGTCAGTGCTCAAAGTCCGGGCCAGTACCGCGGCA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,553 total letters Query= IDP96872.102 Length=1461 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1905 0.0 1_06G_G2_IDP96872.102_T7S.seq 1651 0.0 2_10G_G2_IDP96872.102_T7term.seq 1270 0.0 S R N N F V * L * E G D I H M H H H H H H S S G V D L W S H Query_1 64 TCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCAT 153 1 13 ...N...................................................................................... 102 P Q F E K G T E N L Y F Q S N A V I L V K S L D A D G S P K Query_1 154 CCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGTGATTTTGGTGAAGAGTTTAGACGCGGACGGTAGCCCGAAA 243 0 1 .......................................... 42 1 103 ................................................A......................................... 192 E K R R K G T G K I T L T E V A E F V G V S A M T V S R A L Query_1 244 GAAAAACGACGTAAAGGAACGGGTAAGATAACCCTGACCGAGGTGGCTGAGTTTGTCGGTGTGAGCGCCATGACCGTCTCGCGAGCGCTG 333 0 43 .......................................................................................... 132 1 193 .......................................................................................... 282 R M P E K V N P E L R D R I D A A V S Q L G Y V P N L Q A R Query_1 334 AGAATGCCTGAAAAAGTGAATCCTGAGCTTCGCGATCGCATCGATGCGGCCGTCAGTCAACTCGGCTATGTCCCTAATCTGCAGGCACGT 423 0 133 .......................................................................................... 222 0 180 ............ 169 1 283 .......................................................................................... 372 1 330 ............ 319 N L A S V H S D L I L A V V P T F S S P G F L P V S E A L Query_1 424 AATCTGGCGTCGGTCCATTCCGATCTTATTCTCGCCGTCGTTCCCACCTTCTCTTCGCCGGGTTTTTTGCCGG--TCTCTGAAGCGCT-G 510 0 223 .........................................................................--.............-. 309 1 373 ........C................................................................--.............-. 459 2 914 .....NN....GN...A.....NNA.N. 887 Q K V L T E R G Y T M M F M E S G H D G Q S E Q K Query_1 511 CAAAAA--GT-GCTGA-CCGAA-CGCGGAT-ATACCATGA-TGTTT-ATGGAA-TCCGGG--CATGAC-GGTCAAAG-TGAA--CAAAAG 585 0 310 ......--..-.....-.....-.......-.........-.....-......-......--......-........-....--...... 384 1 460 ......--..-.....-.....-.......-.........-.....-......-......--......-........-....--...... 534 2 886 ......AA..A.A...A...N.N..A....N.N.....N.A.....N..N...A...N..GN..N...N........G....ACN..... 797 T F E K M L A Y N P A A I I Q F N I D T I E S C T Q Query_1 586 A-CGTTTGAAAAAA-TGC--TCGCC-TAT-AACCCGGCGG-CCATTATT-CAG-TTCAATATCG-ATACCATTGAGA-GCTGCACCCAGT 664 0 385 .-............-...--.....-...-..........-........-...-..........-............-............ 463 1 535 .-............-...--.....-...-..........-........-...-..........-............-............ 613 2 796 NN............AN.NCT...N.N...T..........G........T...N..........G..C.........N..........N. 707 L L A N V D V P I L E I G A L N T H P V G M C I G V D Y G K Query_1 665 TGCTGGCCAACGTGGATGTTCCTATTCTGGAAATTGGCGCGCTAAATACCCACCCTGTGGG-TATGTGCATCGGCGTGGATTACGGTAAG 753 0 464 .............................................................-............................ 552 1 614 .......................................................C.....-............................ 702 2 706 .......A....................................T.......N..C.....G......N..N.................. 617 A V K Q I V T H L A D A S L K N I A L L C T P A N N T M F R Query_1 754 GCCGTAAAACAGATCGTGACTCATCTGGCCGACGCCTCATTAAAAAACATCGCACTGCTCTGTACCCCGGCTAATAACACAATGTTTCGC 843 0 553 .......................................................................................... 642 1 703 .................................................................N........................ 792 2 616 ......................................................N................................... 527 Q L L S G W N T A M L A L N R S P H R V V T T H L P S T I A Query_1 844 CAGTTGCTGAGCGGTTGGAATACGGCGATGCTCGCTCTGAACCGCTCCCCGCACCGGGTGGTAACCACGCATTTGCCATCCACCATCGCC 933 0 643 .......................................................................................... 732 1 793 ...-..........A.............................N....T......................................-. 880 2 526 ..............A........................................................................... 437 T G V N I F K D M M I T W G D L D A L I C T S D E M A C G C Query_1 934 ACAGGCGTCAATATATTCAAGGATATGATGATTACCTGGGGCGATCTGGATGCGTTAATTTGCACGTCGGATGAAATGGCCTGTGGATGT 1023 0 733 .......................................................................................... 822 1 881 .........-.....-......................................-............N..........NNNNTG.A.... 967 2 436 .......................................................................................... 347 M M A C H S A G I K V P N T V A I A S L G G G V L S T V C S Query_1 1024 ATGATGGCCTGCCACAGTGCCGGCATTAAGGTGCCCAATACCGTAGCCATTGCGAGTTTAGGCGGAGGAGTGTTATCGACAGTCTGTTCC 1113 0 823 .......................................................................................... 912 1 968 .............N.-NN...-NNN...---GNN...........N...... 1014 2 346 .......................................................................................... 257 P A L T T V E F P W H D I G V K A G K A L L E L L N D K P G Query_1 1114 CCTGCCCTGACAACCGTTGAGTTTCCCTGGCATGACATTGGGGTGAAAGCCGGTAAAGCTCTTCTCGAACTGCTTAACGATAAGCCTGGT 1203 0 913 .......................................................................................... 1002 2 256 .......................................................................................... 167 E K F I E I P S V L K V R A S T A A * H W K W I T D P N S S Query_1 1204 GAAAAGTTCATTGAAATTCCGTCAGTGCTCAAAGTCCGGGCCAGTACCGCGGCATAACATTGGAAGTGGATAACGGATCCGAATTCGAGC 1293 0 1003 ...................................................... 1056 2 166 .......................................................................................... 77 S V D K L A A A L E H H H H H H * D P A A N K A R K E A E Query_1 1294 TCCGTCG-ACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTT 1382 2 76 .......A.......................---.............................N.--C....... 7 L A A A T A E Q * L A * P L G A S K R V L R G F L Query_1 1383 GGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGC 1456 0 282 ........... 292 1 432 ........... 442 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5064725 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 13, 2018 11:15 AM Number of letters in database: 3,553 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96876.102 (IDP96876.1-74.F..pMCSG68)
MGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW
ATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGTGCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATACCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,648 total letters Query= IDP96876.102 Length=627 Score E Sequences producing significant alignments: (Bits) Value 2_11C_C3_IDP96876.102_T7term.seq 944 0.0 1_07C_C3_IDP96876.102_T7S.seq 908 0.0 0_wt 401 2e-115 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAA 90 2 546 .......................................................................................... 457 2 479 ...G...........T.T.C.C.C...........A... 517 1 5 ..N...-................-.....- 31 E G D I H M H H H H H H S S G V D L W S H P Q F E K G T Query_1 91 GAAGGAGATATACATATG-CACCA-TCATCATCA-TCATTCTTCTGG-TGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGA 176 2 456 ..................-.....-.........-............-.......................................... 371 1 32 ..N...............G.....C.........C............A....................................N..... 121 E N L Y F Q S N A M G R I L L D L S D D V I Q R L D D L K V Query_1 177 GAACCTGTACTTCCAATCCAATGCCATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGTGCA 266 2 370 .......................................................................................... 281 1 122 .......................................................................................... 211 0 1 ................................................................. 65 Q R N I P R A E L L R E A V E Q Y L E K Q D R A K D T I S S Query_1 267 GCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATACCATCTCCAGTGC 356 2 280 .......................................................................................... 191 1 212 .................N.........................................N.............................. 301 0 66 .......................................................................................... 155 A L G L W Q D C E E D G M E Y Q R Q L R K E W * H W K W I T Query_1 357 GCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGGTAACATTGGAAGTGGATAACGGA 446 2 190 .......................................................................................... 101 1 302 ............N..................N.......................................................... 391 0 156 ................................................................... 222 D P N S S S V D K L A A A L E H H H H H H * D P A A N K A R Query_1 447 TCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAA-GCCCGAA 535 2 100 ............................................................N.................NN..TN...... 11 1 392 .........T.....................................N........N.........................-.N..... 480 K E A E L A A A T A E Q * L A * P L G A S K R V L R G F L L Query_1 536 AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAAT-AACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTG 624 1 481 ..................................N.A............N.............N......N................... 570 Query_1 625 A 625 1 571 . 571 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1593036 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 13, 2018 11:15 AM Number of letters in database: 2,648 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96877.102 (IDP96877.1-740.F..pMCSG68)
MKKILIMTPDIEGPVRNGGIGTAFTALATTLAKKGYDVDVLYTCGDYSESSVSKFSDWSRIYSTFGINLLRTGLIKEINIDAPYFRRKSYSIYLWLKENNIYDTVISCEWQADLYYTLLSKKNGTDFENTKFIVNTHSSTLWADEGNYQLPYDQNHLELYYMEKMVVEMADEVVSPSQYLIDWMLSKHWNVPEERHVILNCEPFQGFVTRDDVTVKINEKPASGVELVFFGRLETRKGLDIFLRALRKLSDEDKESISGVTFLGKNVTMGKTDSFTYIMNQTKNLGLAVNVISDYDRTNANEYIKRKNVLVIIPSLVENSPYTVYECLINNVNFLASNVGGIPELIPQEHHAEVLFIPTPVDLYGKIHYRLKNINIKPGLAESQDNIKEAWFVAVERKNNRAFKKIDEANSPLVSVCITHFERHHLLQQALASIKSQTYQNIEVILVDDGSTTEDSHRYLNLIENDFNSRGWKIVRSSNNYLGAARNLAARHASGEYLMFMDDDNVAKPFEVETFVTAALNSGADVLTTPSDLIFGEEFPSPFRKMTHCWLPLGPDLNIASFSNCFGDANALIRKEVFEKVGGFTEDYGLGHEDWEFFAKISLQGYKLQIVPEPLFWYRVANSGMLLSGNKSKNNYRSFRPFMDENVKYNYAMGLIPSYLEKIQELESEVNRLRSINGGHSVSNELQLLNNKVDGLISQQRDGWAHDRFNALYEAIHVQGAKRGTSLVRRVARKVKSMLK
ATGAAGAAAATTCTTATAATGACGCCGGACATTGAGGGGCCTGTCCGTAACGGCGGTATTGGTACTGCTTTCACTGCTCTTGCCACTACTTTGGCAAAAAAGGGGTATGATGTTGATGTATTGTATACATGTGGCGACTATTCTGAATCATCTGTATCGAAATTTAGCGACTGGTCACGTATTTATAGTACCTTTGGTATCAATCTGCTAAGAACCGGACTGATAAAAGAGATTAATATTGATGCACCGTATTTTAGAAGGAAAAGTTATTCAATTTATCTCTGGTTGAAAGAAAATAACATCTATGACACTGTTATTTCTTGTGAGTGGCAGGCAGATCTTTATTACACTTTATTAAGCAAAAAGAATGGAACGGATTTTGAAAATACAAAGTTCATTGTAAATACTCACAGTTCAACGTTATGGGCTGATGAAGGTAATTACCAGCTTCCATATGATCAGAACCATCTTGAACTCTATTATATGGAGAAAATGGTGGTTGAAATGGCGGATGAAGTTGTTAGTCCGTCTCAGTATTTAATTGATTGGATGTTGAGTAAGCACTGGAATGTTCCTGAAGAACGTCATGTAATTTTAAATTGCGAGCCATTTCAAGGGTTTGTGACGAGAGATGATGTTACAGTTAAAATAAATGAAAAGCCAGCTTCTGGCGTTGAGCTTGTATTTTTCGGCCGCCTTGAAACCCGTAAAGGACTTGACATATTCCTGCGTGCATTAAGAAAACTATCTGATGAAGATAAAGAGAGCATTTCTGGAGTAACCTTCCTCGGAAAAAATGTCACTATGGGGAAAACTGATTCATTTACTTATATTATGAATCAGACTAAAAATTTGGGACTCGCAGTTAATGTAATCAGCGACTATGATCGTACCAACGCTAATGAATATATAAAAAGAAAAAATGTATTAGTCATCATTCCATCACTTGTAGAAAACTCACCCTATACTGTTTATGAATGCTTGATTAATAACGTTAATTTCCTCGCTTCAAACGTTGGTGGAATTCCAGAGCTTATTCCGCAAGAGCATCATGCGGAAGTTCTATTTATTCCTACACCTGTCGATTTATACGGGAAAATCCACTATCGCTTAAAAAATATAAATATAAAACCAGGGCTTGCTGAATCACAAGACAATATTAAAGAAGCTTGGTTTGTCGCAGTTGAACGAAAAAACAACCGCGCATTCAAGAAAATCGATGAAGCTAACAGCCCGTTAGTTAGCGTGTGTATAACTCACTTCGAACGTCACCATTTGCTTCAGCAAGCACTCGCATCAATAAAATCTCAGACGTACCAAAATATTGAGGTCATCTTGGTTGATGATGGAAGTACGACAGAAGATTCTCATCGTTATTTAAATCTCATCGAGAATGATTTTAACTCTCGAGGCTGGAAAATTGTCCGTAGTTCTAATAACTATCTGGGTGCTGCAAGGAATTTGGCTGCGCGACACGCCTCTGGCGAATATCTGATGTTTATGGACGATGATAATGTTGCTAAGCCTTTTGAGGTAGAAACGTTTGTTACTGCAGCATTAAACTCTGGGGCCGATGTGTTAACCACACCAAGCGATCTTATTTTTGGTGAGGAGTTCCCTTCTCCGTTCCGTAAAATGACGCACTGCTGGCTTCCGTTAGGGCCTGATTTAAATATCGCCAGCTTTAGTAACTGCTTTGGCGATGCTAATGCGCTGATCAGAAAAGAGGTTTTCGAAAAAGTAGGCGGATTTACTGAAGATTACGGTTTAGGTCATGAAGACTGGGAGTTTTTTGCCAAAATATCATTACAGGGATATAAATTGCAAATCGTCCCGGAACCTCTATTTTGGTATAGAGTTGCAAACTCCGGCATGTTGTTAAGTGGAAATAAGAGTAAAAATAACTACCGCAGTTTCCGTCCTTTTATGGATGAGAATGTTAAATATAACTATGCAATGGGGTTGATACCTTCCTACCTCGAGAAGATTCAAGAACTTGAGAGTGAAGTGAATCGCTTGCGGAGCATCAATGGTGGTCATTCTGTCAGTAACGAGTTACAACTTTTAAATAATAAGGTTGATGGTCTTATTTCTCAGCAAAGAGATGGCTGGGCCCATGACCGTTTTAATGCTCTGTATGAAGCAATTCACGTCCAAGGCGCAAAACGAGGCACCAGCCTGGTTCGCCGGGTTGCCCGGAAAGTGAAATCAATGTTAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,763 total letters Query= IDP96877.102 Length=2625 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4004 0.0 2_09C_C1_IDP96877.102_T7term.seq 417 2e-119 1_05C_C1_IDP96877.102_T7S.seq 414 2e-118 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAA 90 2 379 .......................................................................................... 290 2 312 ...G...........T.T.C.C.C...........A... 350 1 8 ..T..........-................... 39 E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E N Query_1 91 GAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAAC 180 2 289 .......................................................................................... 200 1 40 .......................................................................................... 129 L Y F Q S N A M K K I L I M T P D I E G P V R N G G I G T A Query_1 181 CTGTACTTCCAATCCAATGCCATGAAGAAAATTCTTATAATGACGCCGGACATTGAGGGGCCTGTCCGTAACGGCGGTATTGGTACTGCT 270 0 1 ..................................................................... 69 2 199 ..................................................A.G... 144 1 130 ..................................................A.G... 185 F T A L A T T L A K K G Y D V D V L Y T C G D Y S E S S V S Query_1 271 TTCACTGCTCTTGCCACTACTTTGGCAAAAAAGGGGTATGATGTTGATGTATTGTATACATGTGGCGACTATTCTGAATCATCTGTATCG 360 0 70 .......................................................................................... 159 0 1803 ..T............ 1789 K F S D W S R I Y S T F G I N L L R T G L I K E I N I D A P Query_1 361 AAATTTAGCGACTGGTCACGTATTTATAGTACCTTTGGTATCAATCTGCTAAGAACCGGACTGATAAAAGAGATTAATATTGATGCACCG 450 0 160 .......................................................................................... 249 Y F R R K S Y S I Y L W L K E N N I Y D T V I S C E W Q A D Query_1 451 TATTTTAGAAGGAAAAGTTATTCAATTTATCTCTGGTTGAAAGAAAATAACATCTATGACACTGTTATTTCTTGTGAGTGGCAGGCAGAT 540 0 250 .......................................................................................... 339 L Y Y T L L S K K N G T D F E N T K F I V N T H S S T L W A Query_1 541 CTTTATTACACTTTATTAAGCAAAAAGAATGGAACGGATTTTGAAAATACAAAGTTCATTGTAAATACTCACAGTTCAACGTTATGGGCT 630 0 340 .......................................................................................... 429 D E G N Y Q L P Y D Q N H L E L Y Y M E K M V V E M A D E V Query_1 631 GATGAAGGTAATTACCAGCTTCCATATGATCAGAACCATCTTGAACTCTATTATATGGAGAAAATGGTGGTTGAAATGGCGGATGAAGTT 720 0 430 .......................................................................................... 519 V S P S Q Y L I D W M L S K H W N V P E E R H V I L N C E P Query_1 721 GTTAGTCCGTCTCAGTATTTAATTGATTGGATGTTGAGTAAGCACTGGAATGTTCCTGAAGAACGTCATGTAATTTTAAATTGCGAGCCA 810 0 520 .......................................................................................... 609 F Q G F V T R D D V T V K I N E K P A S G V E L V F F G R L Query_1 811 TTTCAAGGGTTTGTGACGAGAGATGATGTTACAGTTAAAATAAATGAAAAGCCAGCTTCTGGCGTTGAGCTTGTATTTTTCGGCCGCCTT 900 0 610 .......................................................................................... 699 E T R K G L D I F L R A L R K L S D E D K E S I S G V T F L Query_1 901 GAAACCCGTAAAGGACTTGACATATTCCTGCGTGCATTAAGAAAACTATCTGATGAAGATAAAGAGAGCATTTCTGGAGTAACCTTCCTC 990 0 700 .......................................................................................... 789 G K N V T M G K T D S F T Y I M N Q T K N L G L A V N V I S Query_1 991 GGAAAAAATGTCACTATGGGGAAAACTGATTCATTTACTTATATTATGAATCAGACTAAAAATTTGGGACTCGCAGTTAATGTAATCAGC 1080 0 790 .......................................................................................... 879 D Y D R T N A N E Y I K R K N V L V I I P S L V E N S P Y T Query_1 1081 GACTATGATCGTACCAACGCTAATGAATATATaaaaagaaaaaaTGTATTAGTCATCATTCCATCACTTGTAGAAAACTCACCCTATACT 1170 0 880 .......................................................................................... 969 V Y E C L I N N V N F L A S N V G G I P E L I P Q E H H A E Query_1 1171 GTTTATGAATGCTTGATTAATAACGTTAATTTCCTCGCTTCAAACGTTGGTGGAATTCCAGAGCTTATTCCGCAAGAGCATCATGCGGAA 1260 0 970 .......................................................................................... 1059 V L F I P T P V D L Y G K I H Y R L K N I N I K P G L A E S Query_1 1261 GTTCTATTTATTCCTACACCTGTCGATTTATACGGGAAAATCCACTATCGCTTAAAAAATATAAATATAAAACCAGGGCTTGCTGAATCA 1350 0 1060 .......................................................................................... 1149 Q D N I K E A W F V A V E R K N N R A F K K I D E A N S P L Query_1 1351 CAAGACAATATTAAAGAAGCTTGGTTTGTCGCAGTTGAACGAAAAAACAACCGCGCATTCAAGAAAATCGATGAAGCTAACAGCCCGTTA 1440 0 1150 .......................................................................................... 1239 V S V C I T H F E R H H L L Q Q A L A S I K S Q T Y Q N I E Query_1 1441 GTTAGCGTGTGTATAACTCACTTCGAACGTCACCATTTGCTTCAGCAAGCACTCGCATCAATAAAATCTCAGACGTACCAAAATATTGAG 1530 0 1240 .......................................................................................... 1329 V I L V D D G S T T E D S H R Y L N L I E N D F N S R G W K Query_1 1531 GTCATCTTGGTTGATGATGGAAGTACGACAGAAGATTCTCATCGTTATTTAAATCTCATCGAGAATGATTTTAACTCTCGAGGCTGGAAA 1620 0 1330 .......................................................................................... 1419 I V R S S N N Y L G A A R N L A A R H A S G E Y L M F M D D Query_1 1621 ATTGTCCGTAGTTCTAATAACTATCTGGGTGCTGCAAGGAATTTGGCTGCGCGACACGCCTCTGGCGAATATCTGATGTTTATGGACGAT 1710 0 1420 .......................................................................................... 1509 D N V A K P F E V E T F V T A A L N S G A D V L T T P S D L Query_1 1711 GATAATGTTGCTAAGCCTTTTGAGGTAGAAACGTTTGTTACTGCAGCATTAAACTCTGGGGCCGATGTGTTAACCACACCAAGCGATCTT 1800 0 1510 .......................................................................................... 1599 I F G E E F P S P F R K M T H C W L P L G P D L N I A S F S Query_1 1801 ATTTTTGGTGAGGAGTTCCCTTCTCCGTTCCGTAAAATGACGCACTGCTGGCTTCCGTTAGGGCCTGATTTAAATATCGCCAGCTTTAGT 1890 0 1600 .......................................................................................... 1689 N C F G D A N A L I R K E V F E K V G G F T E D Y G L G H E Query_1 1891 AACTGCTTTGGCGATGCTAATGCGCTGATCAGAAAAGAGGTTTTCGAAAAAGTAGGCGGATTTACTGAAGATTACGGTTTAGGTCATGAA 1980 0 1690 .......................................................................................... 1779 D W E F F A K I S L Q G Y K L Q I V P E P L F W Y R V A N S Query_1 1981 GACTGGGAGTTTTTTGCCAAAATATCATTACAGGGATATAAATTGCAAATCGTCCCGGAACCTCTATTTTGGTATAGAGTTGCAAACTCC 2070 0 1780 .......................................................................................... 1869 0 101 ............G.. 87 G M L L S G N K S K N N Y R S F R P F M D E N V K Y N Y A M Query_1 2071 GGCATGTTGTTAAGTGGAAATAAGAGTAAAAATAACTACCGCAGTTTCCGTCCTTTTATGGATGAGAATGTTAAATATAACTATGCAATG 2160 0 1870 .......................................................................................... 1959 G L I P S Y L E K I Q E L E S E V N R L R S I N G G H S V S Query_1 2161 GGGTTGATACCTTCCTACCTCGAGAAGATTCAAGAACTTGAGAGTGAAGTGAATCGCTTGCGGAGCATCAATGGTGGTCATTCTGTCAGT 2250 0 1960 .......................................................................................... 2049 N E L Q L L N N K V D G L I S Q Q R D G W A H D R F N A L Y Query_1 2251 AACGAGTTACAACTTTTAAATAATAAGGTTGATGGTCTTATTTCTCAGCAAAGAGATGGCTGGGCCCATGACCGTTTTAATGCTCTGTAT 2340 0 2050 .......................................................................................... 2139 E A I H V Q G A K R G T S L V R R V A R K V K S M L K * H W Query_1 2341 GAAGCAATTCACGTCCAAGGCGCAAAACGAGGCACCAGCCTGGTTCGCCGGGTTGCCCGGAAAGTGAAATCAATGTTAAAATAACATTGG 2430 0 2140 ................................................................................. 2220 2 154 .................................. 121 1 175 .................................. 208 K W I T D P N S S S V D K L A A A L E H H H H H H * D P A A Query_1 2431 AAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCT 2520 2 120 .......................................................................................... 31 1 209 .......................................................................................... 298 N K A R K E A E L A A A T A E Q * L A * P L G A S K R V L Query_1 2521 AACAAAGCCCG-AAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAG 2609 2 30 ...........A....... 12 1 299 ...........-.............................................................................. 387 R G F L L K Query_1 2610 GGGTTTTTTGCTGAAA 2625 1 388 ................ 403 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12288896 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 13, 2018 11:15 AM Number of letters in database: 4,763 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96885.103 (IDP96885.32-441.P(441)..pMCSG68)
SSFDEHLDASAGGGGGSSIDAVMVDPGAVVNNYNRQQQQQASARRAAEQREKQAQQQAEELREKQAAEQERLKQLEQERLQAQEAAKEAKEQQKQAEEAAAKAAAAAKAKADAQAKEAQEAAAKAAAEAKAKADAQAKAAEQAAAKAAADAKKQAEAAAAKAAAEAKKQAEAEAAKAAAEAQKKAEAAAAKKAQQEAEKKAQQEAAKQAAAEKAAAEKAAEKAAAQKAAAEKAAAEKAAAAEKAAAAKAAAAEKAAADKAAKAAAAKAAAAKKAAAAKEADGVDNLLGDLSSGKNAPKTGGGAKGNNASAAGSGNTKNSASGADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGLLLNIQSEGGDPALCQAALAAARQAKFPKPPSQAVYEVFKNAPLDFKPQ
AGTTCGTTTGACGAGCATCTGGATGCCTCTGCCGGCGGCGGCGGCGGATCGTCGATTGATGCCGTCATGGTCGATCCGGGGGCGGTGGTAAATAACTATAACCGTCAGCAACAGCAGCAGGCCAGCGCACGTCGCGCCGCTGAACAGCGTGAAAAACAGGCGCAGCAGCAGGCGGAAGAGTTACGTGAGAAACAGGCGGCGGAACAGGAACGGCTGAAACAGCTCGAACAGGAGCGGCTGCAGGCGCAGGAAGCGGCGAAAGAAGCGAAGGAGCAGCAGAAGCAGGCTGAAGAAGCGGCTGCCAAGGCCGCCGCGGCGGCAAAAGCCAAAGCGGACGCACAGGCAAAAGAAGCGCAGGAAGCCGCTGCCAAAGCGGCCGCCGAGGCGAAAGCGAAGGCGGATGCCCAGGCGAAAGCGGCAGAACAGGCGGCGGCCAAGGCGGCTGCTGACGCGAAAAAGCAGGCCGAAGCCGCTGCAGCGAAAGCCGCTGCCGAGGCGAAGAAACAGGCGGAAGCTGAAGCGGCGAAAGCTGCGGCCGAGGCGCAGAAGAAAGCGGAAGCGGCGGCTGCGAAGAAAGCGCAACAGGAAGCGGAGAAAAAAGCCCAGCAGGAAGCGGCTAAGCAGGCGGCAGCTGAAAAAGCGGCTGCCGAAAAAGCCGCTGAGAAAGCCGCCGCGCAAAAAGCGGCCGCTGAGAAGGCCGCCGCCGAGAAAGCCGCAGCCGCTGAAAAAGCGGCGGCAGCGAAAGCGGCTGCAGCAGAGAAGGCTGCAGCTGATAAAGCGGCCAAAGCGGCAGCAGCAAAAGCCGCGGCGGCGAAGAAAGCGGCGGCTGCGAAAGAAGCGGACGGCGTTGACAACCTGCTCGGCGATCTGAGTTCTGGTAAGAATGCGCCTAAAACAGGCGGTGGGGCCAAAGGAAACAATGCCTCCGCTGCCGGGAGTGGTAATACTAAAAACAGTGCCTCAGGGGCTGATATCAACAACTATGCCGGACAGATAAAATCGGCGATTGAAAGTAAGTTTTATGACGCATCGTCCTATGCGGGCAAAACATGTACCTTGCGTATCAAACTTGCTCCTGACGGCCTGTTGTTAAATATACAGTCCGAAGGTGGTGATCCTGCTCTGTGCCAGGCCGCTCTTGCCGCAGCCCGACAGGCTAAGTTTCCGAAACCACCTAGCCAGGCAGTATATGAAGTCTTCAAAAATGCGCCACTGGACTTCAAACCTCAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,601 total letters Query= IDP96885.103 Length=1635 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2219 0.0 2_09D_D1_IDP96885.103_T7term.seq 1860 0.0 1_05D_D1_IDP96885.103_T7S.seq 1737 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 60 CCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTC 149 1 12 ...T.......-............-...............................---............................... 96 S H P Q F E K G T E N L Y F Q S N A S S F D E H L D A S A G Query_1 150 TCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCAGTTCGTTTGACGAGCATCTGGATGCCTCTGCCGGCGG 239 0 1 ...................................... 38 1 97 .......................................................................................... 186 G G G G S S I D A V M V D P G A V V N N Y N R Q Q Q Q Q A S Query_1 240 CGGCGGCGGATCGTCGATTGATGCCGTCATGGTCGATCCGGGGGCGGTGGTAAATAACTATAACCGTCAGCAACAGCAGCAGGCCAGCGC 329 0 39 .......................................................................................... 128 1 187 .......................................................................................... 276 A R R A A E Q R E K Q A Q Q Q A E E L R E K Q A A E Q E R L Query_1 330 ACGTCGCGCCGCTGAACAGCGTGAAAAACAGGCGCAGCAGCAGGCGGAAGAG-TTACGTGAGAAACAGGCGGCGGAACAGGAACGGCTGA 418 0 129 ....................................................-..................................... 217 0 422 ............ 433 0 500 ............ 511 2 1183 ............G....N..N...........N.......-....NN... 1135 2 859 ............ 848 2 936 ........... 926 1 277 ....................................................-..................................... 365 1 570 ............ 581 1 648 ............ 659 1 1034 ........... 1044 K Q L E Q E R L Q A Q E A A K E A K E Q Q K Q A Query_1 419 AACAGCTCGAACAGGAGCGGCTGCAG--GCGCAG---GAAGCGGCG-AAAGAAGCGAAGGAGCA-GCAG--AAGCAG--GC------TGA 491 0 218 ..........................--......---.........-.................-....--......--..------... 290 0 517 .........-....CT...GCC...GC-....--...A.A--..------G.. 557 0 744 ...........CA.A.A..GCT.C...T.AT-....CG..C..--...G-....--C....------------A. 800 0 583 ...---.......A.-...A....CC..C..G.A..G.CT......--..GGCAGC... 635 0 826 ..T...-......... 840 0 606 ....---........ 617 2 1134 NN-..TNA..NNN.NNNN.N-...N.--.NC...---......T.NN.................-.NN.--......--.N------... 1063 2 842 .........-....CT...CC.AG..--....--...A.A--..------G.. 803 2 617 ...........CA.A.A..GCT.C...T.AT-....CG..C..--...G-....--C....------------A. 561 2 777 ...---.......A.-...A....CC..C..G.A..G.CT......--..GGCAGC... 725 2 535 ..T...-......... 521 1 366 ..........................--......---.........-.................-....--......--..------... 438 1 665 .........-....CT...GCC...GC-....--...A.A--..------G.. 705 1 754 ....---........T-..GC.G...--.C...T-.A.A--....G.CT..------C.. 798 1 731 ...---.......A.-...A....CC..C..G.A..G.CT......--..GGCAGC... 783 1 892 ........... 902 E E A A A K A A A A A K A K A D A Q A K E A Query_1 492 AGAAGCGGCTGCC--AAG---GC------CGCC---------GCGGCGGCAAAAGCCAAAGCGGACGCACAGGCAAAAGAAG-C-GC--A 557 0 291 .............--...---..------....---------........................................-.-..--. 356 0 558 ..CG........G--...AAA..GCAACAG.AA---------....A.AA.......C.GCA...A..---...T..--------..--. 623 0 801 ..CC.....G..G--...---AA------A..G---------.....T..G....---......... 844 0 636 .A...........--G.A---AA------A...---------..T.A.---......---..C.-....-----.....CG.C.-..TG. 692 0 685 ..C....AG--...---..------....---------.. 704 0 320 ..------....---------........ 307 0 802 ...---------........G..---G.......CG..---T..G.......-.-. 840 0 812 ..------....---------..... 802 2 1062 N-..N...T....--.GN---..------.N..---------......................N-..C......N......-.-..--. 999 2 802 ..CG........G--...---AA------A..GCAACAGGAA....A.AA.......C.GCA...A..---...T..--------..--. 737 2 560 ..CC.....G..G--...---AA------A..G---------.....T..G....---......... 517 2 724 .A...........GA..A---..------...T---------.A.------------.....C.C...G..---.....CG.-.C..--T 671 2 676 ..C....AG--...---..------....---------.. 657 2 559 ...---------........G..---G.......CG..---T..G.......-.-. 521 2 549 ..------....---------..... 559 1 439 .............--...---..------....---------........................................-.-..--. 504 1 706 ..CG........G--... 721 1 799 .A....C....AG--..A---..------....---------... 823 1 784 .A........... 796 1 799 .....CC.-.-TG--. 810 1 468 ..------....---------........ 455 Q E A A A K A A A E A K A K A D A Q A K A A E Q A A Query_1 558 G-GAAG-CCGCTGCC---AAAGCGGCCGCCGAGGCGAAAGCGAAGGCGGATGCCCAGGCG---AAAGCG---GCAG---AACAGGCGGCG 633 0 357 .-....-........---..........................................---......---....---........... 432 0 624 .-.CG.-.A....AA---........T.....---A.....CGCT.A.A.A...GCC...CAA......GCC..T.---.GA....C..C 702 0 693 .-A.G.-....C...GAG.....C..A....CT.AA.....---.....-----..-...---......---..---------T..A..A 756 0 778 ..G...---........A.. 794 0 191 ........... 201 2 998 .-..-.-........---......-..........................N........---..-...---..N.---.-......... 927 2 736 .-.CG.-.A....AA---........T.....----.....CGCT.A.A.A...GCC...CAA......---..C.CTG.GA....C..C 659 2 670 .A....G....C...GAG.....C..A....CT.AA......---....-----...-..---......---..---------T..A..A 605 2 583 ..G...---........A.. 567 1 505 .-....-........---..........................................---......---....---........... 580 1 811 .-A...-....C..GCAA...........T... 841 1 339 ........... 349 A K A A A D A K K Q A E A A A A K A A A E A K K Q A E Query_1 634 GCCAAGGCGGCTGCTGACGC-GAAAAAGCAG-----GCCGAAGCCGCTGCAGC-GA-AAGCCGCTGCCGAGGCGAAGAAACAGGCGGAAG 715 0 433 ....................-..........-----.................-..-................................. 514 0 703 ...G..AAA..C..A.C...T........G.CGGCA..GA....G........A..G...--.....---A..TG.T...GC...CA... 787 0 757 .AG.....T..A...------..T.....-.-----...A....G..A.....-A.-.......G..---..........GC..... 826 0 188 ............ 199 0 202 . 202 2 926 ....................-..........-----......N..........-..-................................. 845 2 658 ...G..AAA..C..A.C...T........G.CGGCA..GA....G........A..G...--.....A.CT.---.T...GC...CA... 574 2 604 .AG.....T..A...------..T.....-.-----...A....G..A.....-A.-.......G..---..........GC..... 535 2 1185 ............ 1174 2 1162 ........... 1152 1 581 ....................-..........-----.................-..-................................. 662 1 876 ....G..G.....-..-....G.....A.CA.A..------....T.C.. 917 1 336 ............ 347 1 350 . 350 A E A A K A A A E A Q K K A E A A A A K K A Q Q E A E K K Query_1 716 CTG---AAGCGGCGAAAGCTGCGGCCGAGGCGCAGAAGAAAGCGGAAGCGGCGGCTGCGAAGAAAGCGCAACAGGAAGCGGAGAAAAAAG 802 0 515 ...---.................................................................................... 601 0 250 .---............AA...AAG...CA.......C.G..T....AA........C...---..------C.CC....C.GC..... 325 0 788 .G.---C...A..A.....C.....---....A...---------................AG..... 840 0 247 ..........C....G... 265 2 844 ...---.................-.................................................................. 759 2 573 .G.---C...A..A.....C.....---....A....---------...............AG..... 521 1 663 ...---.................................................................................... 749 1 398 .---............AA...AAG...CA.......C.G..T....AA........C...---..------C.CC....C.GC..... 473 1 918 ...ATA......GC....... 938 1 395 ..........C....G... 413 A Q Q E A A K Q A A A E K A A A E K A A E K A Query_1 803 CCCAGCAGGAAGC---GGCTAA------GCAGGCGGCAGCTGAAAAAGCGGCTgccga---aaaagccgctgag---------aaagccg 871 0 602 .............---......------..............................---.............---------....... 670 0 326 ..A.AGC...C..ACA...A..AGAAGC.....AA..C....CC........C.....GGCG.....GAAG.C.---------G.T...C 406 0 266 .GA..G..C.-..---A.--..------......------....G...........A.---GGCC.....G.C.GCAAAAGCC.....G. 334 2 758 .............---......------..............................---...-.........---------....... 691 1 750 .............---......------..............................---.............---------....... 818 1 474 ..A.AGC...C..ACA...A..AGAAGC.....AA..C....CC........C.....GGCG.....GAAG.C.---------G.T...C 554 1 414 .GA..G..C.-..---A.--..------......------....G...........A.---GGCC.....G.C.GCAAAAGCC.....G. 482 A A Q K A A A E K A A A E K A A A A E K A A A A K A Query_1 872 ccgcgca---aaaagcggccgctgagaaggccgccgccgagaaagccgcagccgctgaaaaagCG---GCGG-----CAG-CGAAAGCGG 949 0 671 .......---.......................................................---....-----...-......... 748 0 407 AG...------......---..A..AC....G..G...A..GCG..T..T.A...-........A---.----------.-.CG....C. 472 0 233 ..... 237 0 335 A...A..GGC.....AA..-..A.-....-....T...---.....G..C....AG.CG......AAG....ATGCC...G......... 418 2 690 .......---.......................................................---....-----...-......... 613 2 892 ..N.C---..T.-----...-.......C. 872 1 819 .......---....................G..................................---....-----...-......... 896 1 555 AG...------......---..A..AC....G..G...A..GCG..T..T.A...-........A---.----------.-.CG....C. 620 1 381 ..... 385 1 483 A...A..GGC.....AA..-..A.-....-....T...---.....G..C....AG.CG......AAG....ATGCC...G......... 566 A A A E K A A A D K A A K A A A A K A A Query_1 950 C------TGCAGCAGAGA------AG--GCTGC---AGCT-----GATaaagc-ggccaa--agc-ggcagcagca----aaagccgcgg 1009 0 749 .------...........------..--.....---....-----........-......--...-..........----.......... 808 0 473 .------......-..-.------..CC.....CGAG..G-----A.G...CA-...GG.--...-T.A...G..G----.....T.... 535 0 238 .------.....--.C.C------..-----.A---...G-----.CG....A-A..G..GG...-A....A....GGCTG...AA.... 298 0 419 .AGAACAG..G..G.CC.------..--..G..---T...GACGC..A.....A....G.--...-C..T.....G----........T. 490 2 612 .------...........------..--.....---....-----........-......--...-..........----.......... 553 2 871 .------...C.AG.C..AGAAAC..--..G.A---....-----.---....-...G..--...-T..--------------....A.. 819 1 897 .------...........------..--.....---....-----........-..G...--...T..........----....T..... 957 1 621 .------......-..-.------..CC.....CGAG..G-----A.G...CA-...GG.--...-T.A...G..G----.....T.... 683 1 386 .------.....--.C.C------..-----.A---...G-----.CG....A-A..G..GG...-A....A....GGCTG...AA.... 446 1 567 .AGAACAG..G..G.CC.------..--..G..---T...GACGC..A.....A....G.--...-C..T.....G----........T. 638 A A K K A A A A K E A D G V D N L L G D L S S G K Query_1 1010 c---ggcgaaga---------aagcggcggctgcgaaag---aagcggacggcgTTGACAACCTGCTCGGCGATCTGAGTTCTGGTAAGA 1084 0 809 .---........---------..................---................................................ 883 0 536 .CGA....C...AGAAAGCGG................GA---..... 579 0 299 .---T..C...G---------CC..C.....G..A....CCA......... 337 0 491 .CGA........---------..CA..... 511 2 552 .---........---------..................---................................................ 478 2 818 .---.CA.....AAGCGG---................GA---..... 781 1 958 .---..NN.N..---------...N.N.-.....N..-----G.N........-NNN.....TGC..............-.......--G 1025 1 684 .CGA....C...AGAAAGCGG................GA---..... 727 1 447 .---T..C...G---------CC..C.....G..A....CCA......... 485 1 639 .CGA........---------..CA..... 659 N A P K T G G G A K G N N A S A A G S G N T K N S A S G A Query_1 1085 ATGCGCCT-AAAACAGGCGGTGGGGCCAAAGGAAACAATGCCTCCGCTGCCGGGAGTGGTAATACTAAAAACAGTG-CCTCAGGGGCTGA 1172 0 884 ........-...................................................................-............. 971 2 477 ........-...................................................................-............. 390 1 1026 ....NT..A...............NNN.....NN..NN...NN.....NG.C.N...-NN..........CNG...C....... 1108 D I N N Y A G Q I K S A I E S K F Y D A S S Y A G K T C T L Query_1 1173 TATCAACAACTATGCCGGACAGATAAAATCGGCGATTGAAAGTAAGTTTTATGACGCATCGTCCTATGCGGGCAAAACATGTACCTTGCG 1262 0 972 .......................................................................................... 1061 2 389 .......................................................................................... 300 R I K L A P D G L L L N I Q S E G G D P A L C Q A A L A A A Query_1 1263 TATCAAACTTGCTCCTGACGGCCTGTTGTTAAATATACAGTCCGAAGGTGGTGATCCTGCTCTGTGCCAGGCCGCTCTTGCCGCAGCCCG 1352 0 1062 .......................................................................................... 1151 2 299 .......................................................................................... 210 R Q A K F P K P P S Q A V Y E V F K N A P L D F K P Q * H W Query_1 1353 ACAGGCTAAGTTTCCGAAACCACCTAGCCAGGCAGTATATGAAGTCTTCAAAAATGCGCCACTGGACTTCAAACCTCAGTAACATTGGAA 1442 0 1152 ............................................................................... 1230 2 209 .......................................................................................... 120 K W I T D P N S S S V D K L A A A L E H H H H H H * D P A A Query_1 1443 GTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAA 1532 2 119 ................-......................................................................... 31 N K A R K E Query_1 1533 C-AAAGCCCG-AAAGGAA 1548 2 30 .A........A....... 13 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5752307 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 13, 2018 11:15 AM Number of letters in database: 3,601 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96887.102 (IDP96887.1-538.F..pMCSG68)
MLAPRCRRVTMCNKTLTLNRTAQQPVELPADYDRLLQSITVLAPVQKISLDIDVTHKAALPAVLESALLLVAQLEIVSPSELGGFFGLADHERQVLVDEMIDTGLVRYNDEGDIATTVKLTMQRREGASDEGIAIEDVENHRDFTFIDLCTGHIQPKCNADLQKGLPELPRKINRSDFTDLIASQFRRFQICLPDTKSKRALRSSKARLYRVNRASVGQSGLKQQISLDIHVHSDPLNGIRLDSRLMDYSLEHTRLMESSGLKTEAINWLHERKLEAASSTLEDYCDLAKDMVIRRYIGRDGKLELGRLLHDRQRRKTSYGNSTMQRMMIGPVYAAPNRNTLIKWAERLPRQQQVHQGIWLGATNELFGASLGLGSFIKQMNTELASGERNSRLNLAFQCDEEFRAQRLITNQFNSRTDGSLRIFPKGRSESHLEILVFPGEYGMAVVQYHAALDPSLGYAGLTLPIGYFTTDPEQVAQLWMQVQQRIQSPVRSADQPEVATDDLNKLLGCAPAELAQMLVDNSEEKLKKLVERFNQH
ATGCTTGCGCCGAGGTGCAGGAGAGTGACTATGTGTAACAAGACCCTTACCTTGAACAGAACAGCGCAGCAACCTGTGGAACTGCCGGCTGATTATGACCGCTTGCTACAAAGTATTACCGTGCTGGCACCAGTACAGAAGATCAGTCTGGACATTGATGTGACGCATAAAGCAGCGCTACCAGCGGTTCTTGAGTCAGCTCTTCTACTAGTGGCGCAACTCGAAATCGTGTCTCCTTCTGAGTTAGGAGGCTTTTTCGGACTCGCTGACCATGAGCGTCAGGTTCTGGTTGATGAAATGATCGACACTGGCCTCGTGCGATACAATGATGAAGGCGATATCGCGACTACAGTCAAGCTGACAATGCAGCGCCGAGAAGGCGCATCGGATGAGGGCATTGCAATTGAGGACGTGGAAAATCACCGTGACTTCACCTTCATCGACCTTTGCACTGGGCACATTCAGCCTAAGTGTAATGCGGACCTGCAGAAAGGTTTGCCCGAGTTGCCACGTAAGATCAATCGCAGTGACTTCACCGATTTGATCGCTAGCCAATTTCGTCGCTTTCAGATTTGCTTACCCGATACCAAGAGCAAGCGCGCACTGCGTAGCTCGAAGGCCCGACTCTACCGAGTGAATCGGGCCTCTGTTGGTCAATCGGGGTTGAAACAGCAGATCTCACTCGATATCCACGTCCATTCCGATCCTCTGAACGGGATACGTCTGGACTCACGGTTGATGGACTATAGCTTAGAGCATACACGCTTGATGGAAAGCTCCGGTCTCAAAACAGAGGCTATTAACTGGCTGCACGAGCGCAAGTTAGAGGCCGCTAGTTCCACACTAGAAGACTATTGTGATCTGGCAAAAGATATGGTGATCCGCCGATATATCGGACGTGATGGTAAGCTGGAACTAGGGCGTTTACTCCATGATCGCCAGCGCAGAAAAACCAGCTATGGTAACAGCACGATGCAACGTATGATGATTGGGCCTGTCTATGCAGCCCCCAATCGTAACACTTTAATTAAGTGGGCTGAACGGTTGCCACGTCAGCAGCAGGTACATCAAGGAATCTGGCTTGGTGCTACCAACGAGTTATTTGGCGCTAGTTTAGGTCTCGGGTCGTTCATCAAACAAATGAACACCGAGCTCGCAAGTGGAGAGCGCAACAGTCGCCTCAATTTGGCATTTCAGTGCGATGAGGAATTTCGGGCACAGCGTTTAATCACCAATCAGTTTAATAGCCGTACCGACGGTAGCCTACGAATTTTCCCAAAAGGCCGTTCTGAGTCGCACTTGGAAATATTGGTTTTCCCTGGCGAGTACGGTATGGCAGTGGTTCAGTACCATGCTGCTTTGGATCCGTCTCTTGGGTATGCTGGCCTTACATTGCCAATCGGCTACTTCACGACGGACCCTGAGCAGGTAGCTCAGTTGTGGATGCAGGTGCAGCAACGAATCCAGTCACCGGTTCGGTCAGCCGACCAGCCCGAGGTAGCGACAGATGATCTTAATAAGCTACTGGGTTGTGCTCCTGCAGAATTGGCACAAATGCTAGTCGACAATTCCGAGGAAAAACTGAAAAAACTGGTGGAAAGATTCAATCAACAC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,112 total letters Query= IDP96887.102 Length=2019 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2911 0.0 1_06H_H2_IDP96887.102_T7S.seq 2021 0.0 2_10H_H2_IDP96887.102_T7term.seq 1965 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W S Query_1 61 CCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCT 150 1 10 ..........-............................................................................... 98 H P Q F E K G T E N L Y F Q S N A M L A P R C R R V T M C N Query_1 151 CATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGCTTGCGCCGAGGTGCAGGAGAGTGACTATGTGTAAC 240 0 1 ....................................... 39 1 99 .......................................................................................... 188 K T L T L N R T A Q Q P V E L P A D Y D R L L Q S I T V L A Query_1 241 AAGACCCTTACCTTGAACAGAACAGCGCAGCAACCTGTGGAACTGCCGGCTGATTATGACCGCTTGCTACAAAGTATTACCGTGCTGGCA 330 0 40 .......................................................................................... 129 1 189 .......................................................................................... 278 P V Q K I S L D I D V T H K A A L P A V L E S A L L L V A Q Query_1 331 CCAGTACAGAAGATCAGTCTGGACATTGATGTGACGCATAAAGCAGCGCTACCAGCGGTTCTTGAGTCAGCTCTTCTACTAGTGGCGCAA 420 0 130 .......................................................................................... 219 1 279 .......................................................................................... 368 L E I V S P S E L G G F F G L A D H E R Q V L V D E M I D T Query_1 421 CTCGAAATCGTGTCTCCTTCTGAGTTAGGAGGCTTTTTCGGACTCGCTGACCATGAGCGTCAGGTTCTGGTTGATGAAATGATCGACACT 510 0 220 .......................................................................................... 309 1 369 .......................................................................................... 458 G L V R Y N D E G D I A T T V K L T M Q R R E G A S D E G I Query_1 511 GGCCTCGTGCGATACAATGATGAAGGCGATATCGCGACTACAGTCAAGCTGACAATGCAGCGCCGAGAAGGCGCATCGGATGAGGGCATT 600 0 310 .......................................................................................... 399 1 459 .......................................................................................... 548 A I E D V E N H R D F T F I D L C T G H I Q P K C N A D L Q Query_1 601 GCAATTGAGGACGTGGAAAATCACCGTGACTTCACCTTCATCGACCTTTGCACTGGGCACATTCAGCCTAAGTGTAATGCGGACCTGCAG 690 0 400 .......................................................................................... 489 0 520 ....G.A........... 537 1 549 .......................................................................................... 638 1 669 ....G.A........... 686 K G L P E L P R K I N R S D F T D L I A S Q F R R F Q I C L Query_1 691 AAAGGTTTGCCCGAGTTGCCACGTAAGATCAATCGCAGTGACTTCACCGATTTGATCGCTAGCCAATTTCGTCGCTTTCAGATTTGCTTA 780 0 490 .......................................................................................... 579 0 418 ....A.C........... 435 1 639 .......................................................................................... 728 1 567 ....A.C........... 584 2 1203 .........NN--.....-........N.NN--..N.N..N..-......-...-. 1156 P D T K S K R A L R S S K A R L Y R V N R A S V G Q S G L Query_1 781 CCCGATACCAAGAGCAAGCGC-GCACTGCGTAGCTCGAAGGCCCGACTCTACCGAGTGAATCGGGCCTCTGTTGGTCAATCGGGGTTGAA 869 0 580 .....................-.................................................................... 668 1 729 .....................-.................................................................... 817 2 1155 N....NN..C..N...-....C..N...............A..N-......N...........N-.NN..N.-.............-... 1071 K Q Q I S L D I H V H S D P L N G I R L D S R L M D Y S L E Query_1 870 ACAGCAGATCTCACTCGATATCCACGTCCATTCCGATCCTCTGAACGGGATACGTCTGGACTCACGGTTGATGGACTATAGCTTAGAGCA 959 0 669 .......................................................................................... 758 1 818 .......................................................................................... 907 2 1070 .........N..........N.................NNN................................................. 981 H T R L M E S S G L K T E A I N W L H E R K L E A A S S T L Query_1 960 TACACGCTTGATGGAAAGCTCCGGTCTCAAAACAGAGGCTATTAACTGGCTGCACGAGCGCAAGTTAGAGGCCGCTAGTTCCACACTAGA 1049 0 759 .......................................................................................... 848 1 908 .......................................................................................... 997 2 980 .......................................................................................... 891 E D Y C D L A K D M V I R R Y I G R D G K L E L G R L L H D Query_1 1050 AGACTATTGTGATCTGGCAAAAGATATGGTGATCCGCCGATATATCGGACGTGATGGTAAGCTGGAACTAGGGCGTTTACTCCATGATCG 1139 0 849 .......................................................................................... 938 0 896 ............ 885 1 998 .......................................................................................... 1087 1 1045 ............ 1034 2 890 .......................................................................................... 801 2 843 ............ 854 R Q R R K T S Y G N S T M Q R M M I G P V Y A A P N R N T L Query_1 1140 CCAGCGCAGAAAAACCAGCTATGGTAACAGCACGATGCAACGTATGATGATTGGGCCTGTCTATGCAGCCCCCAATCGTAACACTTTAAT 1229 0 939 .......................................................................................... 1028 1 1088 ......NN..............................-............... 1140 2 800 .......................................................................................... 711 I K W A E R L P R Q Q Q V H Q G I W L G A T N E L F G A S L Query_1 1230 TAAGTGGGCTGAACGGTTGCCACGTCAGCAGCAGGTACATCAAGGAATCTGGCTTGGTGCTACCAACGAGTTATTTGGCGCTAGTTTAGG 1319 0 1029 .......................................................................................... 1118 2 710 .......................................................................................... 621 G L G S F I K Q M N T E L A S G E R N S R L N L A F Q C D E Query_1 1320 TCTCGGGTCGTTCATCAAACAAATGAACACCGAGCTCGCAAGTGGAGAGCGCAACAGTCGCCTCAATTTGGCATTTCAGTGCGATGAGGA 1409 0 1119 .......................................................................................... 1208 2 620 .......................................................................................... 531 E F R A Q R L I T N Q F N S R T D G S L R I F P K G R S E S Query_1 1410 ATTTCGGGCACAGCGTTTAATCACCAATCAGTTTAATAGCCGTACCGACGGTAGCCTACGAATTTTCCCAAAAGGCCGTTCTGAGTCGCA 1499 0 1209 .......................................................................................... 1298 2 530 .......................................................................................... 441 H L E I L V F P G E Y G M A V V Q Y H A A L D P S L G Y A G Query_1 1500 CTTGGAAATATTGGTTTTCCCTGGCGAGTACGGTATGGCAGTGGTTCAGTACCATGCTGCTTTGGATCCGTCTCTTGGGTATGCTGGCCT 1589 0 1299 .......................................................................................... 1388 2 440 .......................................................................................... 351 L T L P I G Y F T T D P E Q V A Q L W M Q V Q Q R I Q S P V Query_1 1590 TACATTGCCAATCGGCTACTTCACGACGGACCCTGAGCAGGTAGCTCAGTTGTGGATGCAGGTGCAGCAACGAATCCAGTCACCGGTTCG 1679 0 1389 .......................................................................................... 1478 2 350 .......................................................................................... 261 R S A D Q P E V A T D D L N K L L G C A P A E L A Q M L V D Query_1 1680 GTCAGCCGACCAGCCCGAGGTAGCGACAGATGATCTTAATAAGCTACTGGGTTGTGCTCCTGCAGAATTGGCACAAATGCTAGTCGACAA 1769 0 1479 .......................................................................................... 1568 2 260 .......................................................................................... 171 N S E E K L K K L V E R F N Q H * H W K W I T D P N S S S V Query_1 1770 TTCCGAGGAAAAACTGAAAAAACTGGTGGAAAGATTCAATCAACACTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGA 1859 0 1569 .............................................. 1614 2 170 .......................................................................................... 81 D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 1860 CAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGC 1934 2 80 ......................---............................................A..... 9 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 8130122 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 13, 2018 11:15 AM Number of letters in database: 4,112 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96900.102 (IDP96900.1-82.F..pMCSG68)
MPHARPEQGVLRNGLSLLHIKEGVSFGSTENDPIYVVIMLCARSGNEHITMIGELAEIFSDQQKLHRLLNADDSKAIQAVID
ATGCCTCATGCCCGTCCTGAACAGGGGGTATTAAGAAATGGATTATCGCTACTTCATATTAAAGAAGGGGTGTCGTTCGGCTCGACAGAGAACGATCCGATATACGTGGTAATCATGCTATGCGCTCGTTCCGGGAATGAACATATCACGATGATCGGTGAACTGGCTGAAATCTTTAGCGATCAGCAAAAGTTACACCGATTGTTAAATGCCGATGATAGCAAGGCGATTCAGGCCGTTATTGAC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,049 total letters Query= IDP96900.102 Length=651 Score E Sequences producing significant alignments: (Bits) Value 0_wt 444 2e-128 1_07D_D3_IDP96900.102_T7S.seq 354 3e-101 2_11D_D3_IDP96900.102_T7term.seq 352 1e-100 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAA 90 1 16 ............... 30 2 542 .......................................................................................... 453 2 475 ...G...........T.T.C.C.C...........A... 513 E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E N Query_1 91 GAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAAC 180 1 31 .........................---.............................................................. 117 2 452 .............................---.......................................................... 366 L Y F Q S N A M P H A R P E Q G V L R N G L S L L H I K E G Query_1 181 CTGTACTTCCAATCCAATGCCATGCCTCATGCCCGTCCTGAACAGGGGGTATTAAGAAATGGATTATCGCTACTTCATATTAAAGAAGGG 270 0 1 ..................................................................... 69 1 118 ........................ 141 2 365 ........................ 342 V S F G S T E N D P I Y V V I M L C A R S G N E H I T M I G Query_1 271 GTGTCGTTCGGCTCGACAGAGAACGATCCGATATACGTGGTAATCATGCTATGCGCTCGTTCCGGGAATGAACATATCACGATGATCGGT 360 0 70 .......................................................................................... 159 E L A E I F S D Q Q K L H R L L N A D D S K A I Q A V I D * Query_1 361 GAACTGGCTGAAATCTTTAGCGATCAGCAAAAGTTACACCGATTGTTAAATGCCGATGATAGCAAGGCGATTCAGGCCGTTATTGACTAA 450 0 160 ....................................................................................... 246 1 361 ... 363 2 121 ... 119 H W K W I T D P N S S S V D K L A A A L E H H H H H H * D Query_1 451 CATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccacc-acTGAGATCC 539 1 364 ...............................................................................-.......... 452 2 118 ...............................................N...............................A.......... 29 P A A N K A R K E A E L A A A T A E Q * L A * P L G A S K R Query_1 540 GGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCG-CTGAGCAATAACTAG-CATAACCCCTTGGGGCCTCTAAACGG 627 1 453 ...............................................T...............T.......................... 542 2 28 ..........-......... 10 V L R G F L L Query_1 628 GTCTTGAGGGGTTTTTTGCTGAA 650 1 543 ....................... 565 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1910544 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 13, 2018 11:15 AM Number of letters in database: 3,049 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96907.102 (IDP96907.23-138.P(138)..pMCSG68)
KWHGCQEQNLIFFAYNIKHTKAVELCQTEEGYRYTFGPIGKPEIILEKDSNDVTRGGGMAGGFDIKNGDIIYGVMEDKFGNSALIVQRSDYSKVLAKIELDSVSKSYVNKTFEYLH
AAATGGCATGGATGTCAAGAGCAAAACCTTATTTTCTTTGCTTATAACATAAAACATACTAAAGCGGTAGAACTCTGCCAAACGGAGGAAGGTTATAGATACACTTTTGGACCTATTGGAAAACCAGAAATTATTCTAGAAAAAGATAGCAACGATGTTACTCGCGGAGGAGGTATGGCTGGAGGATTTGATATTAAAAATGGTGATATTATTTACGGAGTAATGGAAGATAAATTTGGTAATTCTGCTCTCATCGTACAGAGGTCTGATTATAGTAAAGTCTTGGCGAAAATTGAACTTGATAGTGTAAGCAAGAGCTATGTTAATAAAACCTTTGAATATCTTCAT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,768 total letters Query= IDP96907.102 Length=753 Score E Sequences producing significant alignments: (Bits) Value 0_wt 628 0.0 1_05E_E1_IDP96907.102_T7S.seq 369 1e-105 2_09E_E1_IDP96907.102_T7term.seq 352 1e-100 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L Query_1 1 TCTCGATCCC-GCGAAATTAATACGACTCACTATAGGGGAA-TTGTGAGCGGATAACAA-TTCCCCTCTAGAAATAATTTTGTTTAACTT 87 1 5 ............................ 32 2 759 ..........C..............................A.................-.............................. 671 2 690 ...G.......-....T.T.C.C.C....T.......A... 729 * E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E Query_1 88 TAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAG 177 1 33 .......................................................................................... 122 2 670 .......................................................................................... 581 N L Y F Q S N A K W H G C Q E Q N L I F F A Y N I K H T K A Query_1 178 AACCTGTACTTCCAATCCAATGCCAAATGGCATGGATGTCAAGAGCAAAACCTTATTTTCTTTGCTTATAACATAAAACATACTAAAGCG 267 0 1 .................................................................. 66 1 123 ......................... 147 2 580 ......................... 556 V E L C Q T E E G Y R Y T F G P I G K P E I I L E K D S N D Query_1 268 GTAGAACTCTGCCAAACGGAGGAAGGTTATAGATACACTTTTGGACCTATTGGAAAACCAGAAATTATTCTAGAAAAAGATAGCAACGAT 357 0 67 .......................................................................................... 156 V T R G G G M A G G F D I K N G D I I Y G V M E D K F G N S Query_1 358 GTTACTCGCGGAGGAGGTATGGCTGGAGGATTTGATATTAAAAATGGTGATATTATTTACGGAGTAATGGAAGATAAATTTGGTAATTCT 447 0 157 .......................................................................................... 246 A L I V Q R S D Y S K V L A K I E L D S V S K S Y V N K T F Query_1 448 GCTCTCATCGTACAGAGGTCTGATTATAGTAAAGTCTTGGCGAAAATTGAACTTGATAGTGTAAGCAAGAGCTATGTTAATAAAACCTTT 537 0 247 .......................................................................................... 336 E Y L H * H W K W I T D P N S S S V D K L A A A L E H H H H Query_1 538 GAATATCTTCATTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccac 627 0 337 ............ 348 1 576 .............................................................................. 653 2 127 .............................................................................. 50 H H * D P A A N K A R K E A E L A A A T A E Q * L A * P L Query_1 628 caccacTGAGATCCGGCTGCTAACAAAG-CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGG 716 1 654 ............................-............................................................. 742 2 49 ............................C............ 9 G A S K R V L R G F L L K Query_1 717 GGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAA 753 1 743 ..................................... 779 1 356 ........... 346 2 347 ........... 357 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2009574 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 13, 2018 11:15 AM Number of letters in database: 2,768 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96918.102 (IDP96918.1-350.F..pMCSG68)
MKKVSCVDLFCGAGGLTHGLVLEGVPVVAGIDIDPACKFPYETNNKAKFIEKSVSDFTSSDLNELYADSDVRILAGCAPCQPFSSYARRYEHGGKDGKWGLLYEFSRLAEETRPDLITMENVPSVARHEVFSDFVDTLKRIGYHVWFNVIDCTHYDVPQMRKRMVLLASLHGEIKLAESTCTQPITVRQAISDLPALKAGETSSGDKLHVTSTLSEKNLQRIKASKPGGTWRDWPEHLVADCHRTDRGKTYSSVYGRMEWDKPAPTMTTQCYGFGNGRFGHPEQDRAISLREAAILQSFPQDYVFIPDDSNVNLRVLGRMIGNAVPVKLGQAIARSINSHLDDLNLVATK
ATGAAAAAAGTATCTTGTGTTGATTTATTTTGTGGTGCAGGTGGGTTAACACATGGTCTTGTGCTTGAAGGAGTACCTGTTGTCGCAGGTATTGATATTGATCCTGCCTGCAAGTTTCCCTATGAGACTAATAATAAAGCAAAATTTATTGAGAAAAGCGTAAGCGATTTTACATCTTCAGACTTGAATGAGCTCTACGCTGATTCAGATGTAAGGATTCTTGCTGGCTGCGCACCTTGCCAACCGTTCTCAAGCTATGCAAGGCGTTATGAGCATGGTGGTAAGGATGGTAAGTGGGGGCTGTTGTACGAGTTTTCTCGCTTGGCTGAGGAAACTCGCCCTGATCTGATCACTATGGAGAATGTACCTTCCGTGGCAAGACATGAAGTTTTCAGCGACTTTGTCGATACCTTAAAACGTATTGGCTATCACGTTTGGTTTAATGTCATTGATTGCACTCACTATGACGTCCCCCAGATGCGTAAGCGCATGGTACTGCTTGCTTCTTTACACGGTGAGATAAAACTCGCCGAGTCAACGTGCACTCAGCCAATTACAGTGAGACAAGCCATAAGCGATCTGCCAGCTTTAAAAGCAGGCGAAACTAGTTCTGGGGATAAGCTACATGTGACTTCAACGTTGTCGGAAAAAAATCTGCAACGTATTAAAGCTTCAAAACCCGGTGGAACGTGGCGTGACTGGCCTGAACATTTAGTTGCTGATTGCCATCGTACAGATAGAGGAAAAACATACTCTAGTGTCTATGGTCGAATGGAATGGGATAAACCTGCTCCGACTATGACTACTCAGTGTTATGGTTTCGGGAATGGCCGCTTTGGTCACCCAGAGCAAGATCGTGCAATCTCATTGAGAGAAGCTGCTATTCTTCAGAGTTTTCCGCAGGACTACGTATTTATCCCCGATGATAGCAATGTCAATCTTAGAGTTCTGGGCCGAATGATTGGAAATGCAGTTCCGGTAAAGCTGGGTCAGGCAATCGCTCGCAGTATAAATTCACACCTTGACGATTTGAACTTGGTCGCAACAAAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,926 total letters Query= IDP96918.102 Length=1455 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1894 0.0 2_10E_E2_IDP96918.102_T7term.seq 1117 0.0 1_06E_E2_IDP96918.102_T7S.seq 850 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 56 AATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCA-TTCTTCTGGTGTAGATCTG 144 1 5 .........N---.....-.....N..T..C..N.N..................................C................... 90 W S H P Q F E K G T E N L Y F Q S N A M K K V S C V D L F Query_1 145 TGGTCTCATCCGCAG-TTCGAAAAGGGTACCGAGAACCTGTACTT-CCAATCCAATGCCATGAAAAAAGTATCTTGTGTTGATTTATTTT 232 0 1 ............................... 31 1 91 ...............A.............................T............................................ 180 C G A G G L T H G L V L E G V P V V A G I D I D P A C K F P Query_1 233 GTGGTGCAGGTGGGTTAACACATGGTCTTGTGCTTGAAGGAGTACCTGTTGTCGCAGGTATTGATATTGATCCTGCCTGCAAGTTTCCCT 322 0 32 .......................................................................................... 121 1 181 ....A..................................................................................... 270 Y E T N N K A K F I E K S V S D F T S S D L N E L Y A D S D Query_1 323 ATGAGACTAATAATAAAGCAAAATTTATTGAGAAAAGCGTAAGCGATTTTACATCTTCAGACTTGAATGAGCTCTACGCTGATTCAGATG 412 0 122 .......................................................................................... 211 1 271 .......................................................................................... 360 V R I L A G C A P C Q P F S S Y A R R Y E H G G K D G K W G Query_1 413 TAAGGATTCTTGCTGGCTGCGCACCTTGCCAACCGTTCTCAAGCTATGCAAGGCGTTATGAGCATGGTGGTAAGGATGGTAAGTGGGGGC 502 0 212 .......................................................................................... 301 1 361 ....................N.................................................N................... 450 L L Y E F S R L A E E T R P D L I T M E N V P S V A R H Query_1 503 TGTTGTACGAGTTTTCTCGCTTGGCTGAGGAAACT-CGCCCTGATCTGATCACTATGGAGA-ATGTACCTT-CCGTGGCAAG-AC-AT-- 585 0 302 ...................................-.........................-.........-..........-..-..-- 384 2 835 .....N.........TN..N.....NN..N.N-- 804 1 451 .....N......................T......C.............-...N.......-.........C..........-..-N.TG 536 E V F S D F V D T L K R I G Y H V W F N V I D C Query_1 586 GAAGTTTT--CAG-CG-A-CTTTG-TC-G--ATACCTT--AAAA--CG-TATTGG-CTATCACGTTTGGTTT-AATGTCATTGATTGCAC 659 0 385 ........--...-..-.-.....-..-.--.......--....--..-......-................-................. 458 2 803 ......G.NC...-N.N.-N.N..-N.-CGA..N..N.--TN..TN..-N.....GNN.........N.N..N..N.....N..N..... 721 1 537 A.....N.--.NNC..-.C..N..N..N.--.......TAC...--..T.....N-..... 589 T H Y D V P Q M R K R M V L L A S L H G E I K L A E S T C Query_1 660 TCACTA-TGA-CGTCC-CCCAGATGCG-TAAG-CGCATGGTACTGCTTGCTTCTTTACACGGTGAGATAAAACTCGCCGAGTCAACGTGC 744 0 459 ......-...-.....-..........-....-......................................................... 543 2 720 ......A...N.....G.......N.NA....A..........A..N.......N.N.....N....A..G...N.G.....N....... 631 T Q P I T V R Q A I S D L P A L K A G E T S S G D K L H V Query_1 745 ACTCAGCCAATTAC-AGTGAGACAAGCCATAAGCGATCTGCCAGCTTTAAAA-GCAGG-CGAAACTAGTTCTGGGGATAAGCTACATGTG 831 0 544 ..............-.....................................-.....-............................... 630 2 630 NN............N.N...........N.....................N.N.....N...G........................... 541 T S T L S E K N L Q R I K A S K P G G T W R D W P E H L V A Query_1 832 ACTTCAACGTTGTCGGaaaaaaaTCTGCAACGTATTAAAGCTTCAAAACCCGGTGGAACGTGGCGTGACTGGCCTGAACATTTAGTTGCT 921 0 631 .......................................................................................... 720 2 540 ..............................N.......N................................................... 451 D C H R T D R G K T Y S S V Y G R M E W D K P A P T M T T Q Query_1 922 GATTGCCATCGTACAGATAGAGGAAAAACATACTCTAGTGTCTATGGTCGAATGGAATGGGATAAACCTGCTCCGACTATGACTACTCAG 1011 0 721 .......................................................................................... 810 2 450 .......................................................................................... 361 C Y G F G N G R F G H P E Q D R A I S L R E A A I L Q S F P Query_1 1012 TGTTATGGTTTCGGGAATGGCCGCTTTGGTCACCCAGAGCAAGATCGTGCAATCTCATTGAGAGAAGCTGCTATTCTTCAGAGTTTTCCG 1101 0 811 .......................................................................................... 900 2 360 ...............................T.......................................................... 271 Q D Y V F I P D D S N V N L R V L G R M I G N A V P V K L G Query_1 1102 CAGGACTACGTATTTATCCCCGATGATAGCAATGTCAATCTTAGAGTTCTGGGCCGAATGATTGGAAATGCAGTTCCGGTAAAGCTGGGT 1191 0 901 .......................................................................................... 990 2 270 .......................................................................................... 181 Q A I A R S I N S H L D D L N L V A T K * H W K W I T D P Query_1 1192 CAGGCAATCGCTCGCAGTATAAATTCACACCTTGACGATTTGAACTTGGTCGCAACAAAGTAACATTGGAA-GTGGATAACGGATCCGAA 1280 0 991 ............................................................ 1050 2 180 .......................................................................T.................. 91 N S S S V D K L A A A L E H H H H H H * D P A A N K A R Query_1 1281 TTCGAGCTCCGTCGA-CAA-GCTTGCGGCCGCA-CTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGA 1362 2 90 ...............G...N.............T.............T.....G...N................--N........ 8 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5580442 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 13, 2018 11:15 AM Number of letters in database: 3,926 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96930.102 (IDP96930.24-382.P(382)..pMCSG68)
GKTSILLVTDKNVGALEATQAIHRLLAAEGREVEVIDSVPAEPNHHDVTQIVSQLGASQPQMVVGIGGGSVLDVAKLLSVLLHPEAPSLTSLLAGEQPQRRICSLLIPATAGTGSEATPNAILAIPEQQTKVGIISPVLLPDYVALLPELTTSMPPSIAASTGIDALCHLLECFTSTVANPVSDNAALIGLHKLVRHIERSVNQPQDLTAKLEMLWASWYGGAAINYSGTHLVHALSYPLGGTWHLPHGVANAILLAPCMRVVRPHAVAKFAQVWDLIPDADRTLSAEEKSHALVAWLAALVKRLPLPDNLAALGVPQESISALSAAAQNVKRLMNNAPCSVSREEIAAIYQTLYPEHA
GGTAAAACCTCAATCCTGCTGGTGACCGACAAGAACGTCGGGGCGCTGGAGGCAACCCAGGCGATTCATCGCCTGCTGGCGGCTGAAGGGCGTGAAGTTGAGGTCATTGACAGCGTGCCAGCGGAGCCCAACCATCACGATGTTACGCAGATCGTCAGCCAGCTGGGCGCCAGCCAGCCGCAGATGGTCGTTGGTATCGGCGGGGGGAGCGTACTGGATGTAGCAAAACTGCTGTCAGTACTTCTGCACCCTGAGGCGCCCTCGCTGACGTCCCTGCTGGCAGGCGAGCAGCCGCAACGACGAATTTGCTCATTGTTAATCCCGGCCACCGCGGGCACCGGATCCGAAGCGACGCCGAACGCCATTCTGGCGATCCCCGAGCAGCAGACGAAGGTGGGGATCATCTCCCCGGTGCTGCTTCCGGACTATGTCGCCCTGCTGCCGGAGCTGACCACCAGCATGCCGCCCAGCATCGCGGCGTCGACCGGAATCGATGCTCTGTGTCATCTGCTGGAGTGCTTTACCTCCACCGTCGCCAACCCGGTGAGCGATAACGCGGCGTTAATCGGTTTACACAAGCTGGTACGCCATATCGAGCGTTCGGTAAATCAGCCGCAGGATCTGACGGCGAAGCTGGAGATGCTCTGGGCCTCGTGGTACGGCGGCGCAGCGATCAACTATTCCGGCACTCACCTGGTGCATGCGCTCTCCTATCCGCTTGGCGGTACCTGGCACCTGCCGCACGGGGTGGCCAACGCCATTCTACTGGCGCCCTGCATGCGGGTGGTCCGCCCTCACGCGGTGGCGAAGTTCGCTCAAGTCTGGGACCTGATCCCCGATGCAGATCGCACGCTCAGCGCGGAAGAGAAATCCCATGCGCTGGTCGCATGGCTGGCGGCGCTGGTCAAACGACTGCCGCTGCCGGACAACCTGGCCGCGCTAGGCGTGCCTCAGGAGAGTATTTCCGCCCTCAGCGCAGCGGCGCAAAACGTCAAACGCCTGATGAACAATGCCCCATGCAGCGTCAGCCGCGAAGAGATCGCGGCCATCTACCAAACGCTGTATCCAGAACATGCC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,561 total letters Query= IDP96930.102 Length=1482 Score E Sequences producing significant alignments: (Bits) Value 2_09F_F1_IDP96930.102_T7term.seq 1954 0.0 0_wt 1943 0.0 1_05F_F1_IDP96930.102_T7S.seq 1864 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 57 ATTCCCC--TCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTG 144 1 2 .......CT...N...-......................................................................... 90 W S H P Q F E K G T E N L Y F Q S N A G K T S I L L V T D K Query_1 145 TGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGGTAAAACCTCAATCCTGCTGGTGACCGACAAG 234 2 1192 ...NN.N............. 1173 0 1 ................................. 33 1 91 .......................................................................................... 180 N V G A L E A T Q A I H R L L A A E G R E V E V I D S V Query_1 235 AACGT-CGGGGC-GCTGGAGGCAACCCAGGCGATTC-ATCGCCTGCTGGCGGCTGAA-GGGCGTGAAGTTGAGGTCATTGACAGCGTGCC 320 2 1172 .NNN.T......N...N...N..C..AG...NN..NN...N..NNN...........A.....N............CA............ 1083 0 34 .....-......-.......................-....................-................................ 119 1 181 .....-......-.......................-....................-................................ 266 P A E P N H H D V T Q I V S Q L G A S Q P Q M V V G I G G G Query_1 321 AGCGGAGCCCAACCATCACGATGTTACGCAGATCGTCAGCCAGCTGGGCGCCAGCCAGCCGCAGA-TGGTCGTTGGTATCGGCGGGGGGA 409 2 1082 ..........NC.....N...............................N.............N.A................N...A... 993 0 120 .................................................................-........................ 208 1 267 .................................................................-........................ 355 1 757 ..........-. 767 S V L D V A K L L S V L L H P E A P S L T S L L A G E Q P Q Query_1 410 GCGTACTGGATGTAGCAAAACTGCTGTCAGTACTTCTGCACCCTGAGGCGCCCTCGCTGACGTCCCTGCTGGCAGGCGAGCAGCCGCAAC 499 2 992 ........................................A...............................................-. 904 0 209 .......................................................................................... 298 1 356 .......................................................................................... 445 R R I C S L L I P A T A G T G S E A T P N A I L A I P E Q Q Query_1 500 GACGAATTTGCTCATTGTTAATCCCGGCCACCGCGGGCACCGGATCCGAAGCGACGCCGAACGCCATTCTGGCGATCCCCGAGCAGCAGA 589 2 903 .......................................................................................... 814 2 448 ........... 438 0 299 .......................................................................................... 388 0 754 ........... 764 1 446 .......................................................................................... 535 T K V G I I S P V L L P D Y V A L L P E L T T S M P P S I A Query_1 590 CGAAGGTGGGGATCATCTCCCCGGTGCTGCTTCCGGACTATGTCGCCCTGCTGCCGGAGCTGACCACCAGCATGCCGCCCAGCATCGCGG 679 2 813 .......................................................................................... 724 0 389 .......................................................................................... 478 1 536 .......................................................................................... 625 A S T G I D A L C H L L E C F T S T V A N P V S D N A A L I Query_1 680 CGTCGACCGGAATCGATGCTCTGTGTCATCTGCTGGAGTGCTTTACCTCCACCGTCGCCAACCCGGTGAGCGATAACGCGGCGTTAATCG 769 2 723 .......................................................................................... 634 0 479 .......................................................................................... 568 1 626 .......................................................................................... 715 G L H K L V R H I E R S V N Q P Q D L T A K L E M L W A S W Query_1 770 GTTTACACAAGCTGGTACGCCATATCGAGCGTTCGGTAAATCAGCCGCAGGATCTGACGGCGAAGCTGGAGATGCTCTGGGCCTCGTGGT 859 2 633 .......................................................................................... 544 0 569 .......................................................................................... 658 1 716 .................................................-........................................ 804 Y G G A A I N Y S G T H L V H A L S Y P L G G T W H L P H G Query_1 860 ACGGCGGCGCAGCGATCAACTATTCCGGCACTCACCTGGTGCATGCGCTCTCCTATCCGCTTGGCGGTACCTGGCACCTGCCGCACGGGG 949 2 543 .......................................................................................... 454 0 659 .......................................................................................... 748 1 805 .......................................................................................... 894 V A N A I L L A P C M R V V R P H A V A K F A Q V W D L I P Query_1 950 TGGCCAACGCCA-TTCTACTGGCGCCCTGCATGCGGGTGGTCCGCCCTCACGCGGTGGCGAAGTTCGCTCAAGTCTGGGACCTGATCCCC 1038 2 453 ............-............................................................................. 365 2 844 .......-.... 834 0 749 ............-............................................................................. 837 0 358 .......-.... 368 1 895 ............T.....................-...................................-................... 982 1 505 .......-.... 515 D A D R T L S A E E K S H A L V A W L A A L V K R L P L P D Query_1 1039 GATGCAGATCGCACGCTCAGCGCGGAAGAGAAATCCCATGCGCTGGTCGCATGGCTGGCGGCGCTGGTCAAACGACTGCCGCTGCCGGAC 1128 2 364 .......................................................................................... 275 0 838 .......................................................................................... 927 1 983 ...........N...........--NN..........................--NN..-.........-.................... 1066 N L A A L G V P Q E S I S A L S A A A Q N V K R L M N N A P Query_1 1129 AACCTGGCCGCGCTAGGCGTGCCTCAGGAGAGTATTTCCGCCCTCAGCGCAGCGGCGCAAAACGTCAAACGCCTGATGAACAATGCCCCA 1218 2 274 .......................................................................................... 185 0 928 .......................................................................................... 1017 1 1067 -....-.N................--N..-N.........-......-...CNN..----N.N........-.N.....--C..NG.... 1142 C S V S R E E I A A I Y Q T L Y P E H A * H W K W I T D P N Query_1 1219 TGCAGCGTCAGCCGCGAAGAGATCGCGGCCATCTACCAAACGCTGTATCCAGAACATGCCTAACATTGGAAGTGGATAACGGATCCGAAT 1308 2 184 .......................................................................................... 95 0 1018 ............................................................ 1077 1 1143 N...T....... 1154 S S S V D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 1309 TCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGC 1397 2 94 ....................................---........................................CG..A..... 9 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5150058 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 13, 2018 11:15 AM Number of letters in database: 3,561 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96931.103 (IDP96931.1-494.F..pMCSG73)
MEPFLSLRHVSKTFHANRALNDISIDFMPGEVHCLAGQNGCGKSTLIKIISGVYRPDEGAAMTLGGKTWPKLTPAASVAHGIQVIYQDLSLFPNLSVWENIAINHYHRGVLVRRSELRRSAEQAMRSINVSLPLDAAVESLSIARCQLVAICRALAQDARLIIMDEPTASLTHQEVEGLLHVVRQLRESGICIVFVSHRLEEVMAVSDRISVLKDGNLVGTMPAAEVTTRRLGFLMTGQEFDFQVRDLWQGQGSTPVLEVRNLSRKGEYHNVSLKVEAGEVVSIVGLLGAGRTELCLSLFGMTRPDSGEILINGQPVRLRSNQDAIRHGIGYVSEDRMSRGLVMSQSIENNIISTVFHKVKGACGLLSDSKAQQLVARMVNALTIKAPDTHLPVNTLSGGNAQRVSIAKWLAISPKLLILDSPTVGVDIANKAGIYHIISDLAAHGIAVLMICDEIEEAWYQSHRILVMKQGELTHSFLPDSSTQQQIAEVVNG
ATGGAACCTTTCCTTTCCCTTCGACACGTCAGCAAAACCTTCCACGCCAACCGCGCGCTCAATGATATCAGCATCGACTTTATGCCCGGAGAAGTGCACTGCCTGGCCGGGCAGAACGGTTGCGGGAAGTCGACGTTGATCAAAATTATCTCTGGCGTCTATCGCCCGGATGAGGGCGCAGCGATGACGCTGGGCGGCAAGACGTGGCCCAAACTGACGCCAGCGGCCTCTGTCGCCCATGGGATCCAGGTGATTTATCAGGATCTGTCGCTGTTTCCCAATCTCAGCGTGTGGGAGAACATTGCTATAAACCACTATCATCGCGGCGTGCTGGTGAGGCGCAGCGAACTGCGACGCAGTGCGGAGCAGGCGATGCGCAGTATTAACGTCTCGCTGCCGCTGGATGCCGCCGTGGAATCGCTCTCCATCGCTCGCTGTCAGCTGGTGGCTATCTGCCGCGCGCTGGCGCAGGATGCCCGGCTTATCATTATGGATGAACCCACGGCCTCATTGACCCATCAGGAAGTGGAGGGACTATTGCATGTGGTGCGCCAGCTGCGTGAGAGTGGGATCTGCATCGTCTTCGTAAGCCACCGTCTGGAAGAGGTCATGGCCGTATCGGACCGCATCAGCGTGCTGAAGGATGGCAATCTGGTCGGCACCATGCCGGCGGCAGAGGTAACCACCCGCCGGCTCGGTTTTCTGATGACGGGCCAGGAGTTTGACTTTCAGGTGCGCGATCTGTGGCAGGGGCAGGGCAGTACGCCGGTGCTGGAGGTGCGCAATCTCAGCCGCAAAGGGGAATATCATAACGTCTCCCTGAAGGTCGAAGCCGGAGAAGTGGTGTCGATAGTCGGCCTGTTGGGCGCCGGGCGAACAGAGCTGTGCCTGAGCCTTTTCGGCATGACCCGCCCTGACAGCGGCGAGATCCTGATAAATGGCCAGCCGGTGCGGTTGCGTAGCAATCAGGACGCTATCCGCCACGGTATCGGCTATGTCTCCGAGGACCGTATGTCGCGCGGCCTGGTGATGAGTCAGTCGATTGAAAACAACATTATCAGCACCGTGTTTCATAAAGTGAAAGGCGCTTGCGGACTATTGAGTGATAGTAAAGCTCAGCAGCTGGTCGCAAGAATGGTCAACGCGTTGACCATTAAGGCGCCGGATACGCATCTGCCGGTGAATACCCTCTCCGGTGGTAATGCCCAGCGCGTTTCTATCGCCAAATGGCTGGCGATCTCTCCTAAGCTGCTGATCCTCGATAGCCCAACCGTGGGGGTGGATATCGCAAACAAAGCCGGGATCTATCATATCATCAGCGATTTGGCTGCCCATGGTATCGCCGTGCTGATGATTTGCGACGAGATTGAAGAGGCGTGGTACCAGAGTCATCGCATCCTGGTGATGAAGCAGGGCGAGTTGACCCATAGCTTCCTGCCGGACAGCAGTACCCAGCAGCAGATAGCGGAGGTGGTCAATGGC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,069 total letters Query= IDP96931.103 Length=1887 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2673 0.0 1_05A_A1_IDP96931.103_v73F.seq 1838 0.0 2_09A_A1_IDP96931.103_T7Term.seq 1755 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 1 41 ...........A........G....------....................NN. 88 1 23 .....C........... 39 A M E P F L S L R H V S K T F H A N R A L N D I S I D F M P Query_1 199 GCCATGGAACCTTTCCTTTCCCTTCGACACGTCAGCAAAACCTTCCACGCCAACCGCGCGCTCAATGATATCAGCATCGACTTTATGCCC 288 0 1 ....................................................................................... 87 0 833 .. 834 1 89 .......................................................................................... 178 1 925 .. 926 2 714 .. 713 G E V H C L A G Q N G C G K S T L I K I I S G V Y R P D E G Query_1 289 GGAGAAGTGCACTGCCTGGCCGGGCAGAACGGTTGCGGGAAGTCGACGTTGATCAAAATTATCTCTGGCGTCTATCGCCCGGATGAGGGC 378 0 88 .......................................................................................... 177 0 835 ......... 843 1 179 .......................................................................................... 268 1 927 ......... 935 2 712 ......... 704 A A M T L G G K T W P K L T P A A S V A H G I Q V I Y Q D Query_1 379 GCAGCGATGACGCTGGGCGGCAAGACGTGGCCCAAACTGACGCCAGCGGCCTCTGTCGCCCATGGGATCCAGGTGATTTATCAGGATCT- 467 0 178 .........................................................................................- 266 0 939 ..............C 925 1 269 .........................................................................................- 357 2 608 ..............C 622 L S L F P N L S V W E N I A I N H Y H R G V L V R R S E L R Query_1 468 GTCGCTGTTTCCCAATCTCAGCGTGTGGGAGAACATTGCTATAAACCACTATCATCGCGGCGTGCTGGTGAGGCGCAGCGAACTGCGACG 557 0 267 .......................................................................................... 356 0 924 .C...... 917 1 358 .......................................................................................... 447 2 1196 . 1196 2 623 .C...... 630 R S A E Q A M R S I N V S L P L D A A V E S L S I A R C Q L Query_1 558 CAGTGCGGAGCAGGCGATGCGCAGTATTAACGTCTCGCTGCCGCTGGA-TGCCGCCGTGGAATCGCTCTCCATCGCTCG-CTGTCAGCTG 645 0 357 ................................................-..............................-.......... 444 1 448 ................................................-..............................-.......... 535 2 1195 ...NNN.N..........NNN.........N..N.N..N..-......A.N..NN...N.....NN....-........G.......... 1108 V A I C R A L A Q D A R L I I M D E P T A S L T H Q E V E Query_1 646 GTGGCTATCTGCCGCGCGCTGGCGCAGG-ATGCCCGGCTTATCATTATGGATGAACCCACGGCCTCATTGACCCATCAGGAAG-TGGAGG 733 0 445 ............................-......................................................-...... 532 1 536 ............................-......................................................-...... 623 2 1107 .GN..................-....N.N............................................-.........N...... 1020 G L L H V V R Q L R E S G I C I V F V S H R L E E V M A V Query_1 734 GACTATTGCA-TGTGGTGCGCCAGCTGCGTG-AGAGTGGGATCTGCATCGTCTTCGTAAGCCACCGTCTGGAAGAGGTCATGGCCGTATC 821 0 533 ..........-....................-.......................................................... 620 1 624 ..........-....................-.......................................................... 711 2 1019 ..........N....................G.......................................................... 930 S D R I S V L K D G N L V G T M P A A E V T T R R L G F L M Query_1 822 GGACCGCATCAGCGTGCTGAAGGATGGCAATCTGGTCGGCACCATGCCGGCGGCAGAGGTAACCACCCGCCGGCTCGGTTTTCTGATGAC 911 0 621 .......................................................................................... 710 1 712 .......................................................................................... 801 2 929 ..N...................................-............................N.......-......----...G 846 T G Q E F D F Q V R D L W Q G Q G S T P V L E V R N L Query_1 912 GGGCCAGGAGTT----TGAC-TTTCAG---GTGCGCGATCTGTGGCAGGGGCAGGG-CAGTACGCCGGTGCTGGAGGTGCGCAATCTCAG 992 0 711 ............----....-......---..........................-................................. 791 1 802 ............----....T......---..........................-....N............................ 883 2 845 NN.N....N...GNTT.T.T-...TT.AGA...AA.NN....G..........N..N..N.N.C.....T.NCA................ 757 S R K G E Y H N V S L K V E A G E V V S I V G L L G A G R T Query_1 993 CCGCAAA-GGGGAATATCATAACGTCTCCCTGAAGG-TCGAAGCCGGAGAAGTGGTGTCGATAGTCGGCCTGTTGGGCGCCGGGCGAACA 1080 0 792 .......-............................-..................................................... 879 0 86 ........... 96 1 884 ....-..-............................N.....................................T............... 971 1 177 ........... 187 2 756 .......A............................-..................................................... 668 E L C L S L F G M T R P D S G E I L I N G Q P V R L R S N Query_1 1081 GAGCTGTGCCTGAGCCTTTTCGGCATGACCCGCCCTGACAGCGGCGAGATCCTGATAAATGGCCAGCCG-GTGCGGTTGCGTAGCAATCA 1169 0 880 .....................................................................-.................... 968 0 274 ......A.-.............. 253 1 972 ............................................N......T.................AT.....-............. 1060 1 365 ......A.-.............. 344 2 667 .....................................................................-A................... 579 Q D A I R H G I G Y V S E D R M S R G L V M S Q S I E N N I Query_1 1170 GGACGCTATCCGCCACGGTATCGGCTATGTCTCCG-AGGACCGTATGTCGC-GCGGCCTGGTGATGAGTCAGTCGATTGAAAACAACATT 1257 0 969 ...................................-...............-...................................... 1056 0 1398 ........... 1408 1 1061 ...NN.......N......................A.......N....TN.NNN...N..NN.....N..........N....-..... 1148 2 578 ...................................-...............-...................................... 491 2 149 ........... 139 I S T V F H K V K G A C G L L S D S K A Q Q L V A R M V N A Query_1 1258 ATCAGCACCGTGTTTCATAAAGTGAAAGGCGCTTGCGGACTATTGAGTGATAGTAAAGCTCAGCAGCTGGTCGCAAGAATGGTCAACGCG 1347 0 1057 .......................................................................................... 1146 0 1155 ............. 1143 2 490 .......................................................................................... 401 2 392 ............. 404 L T I K A P D T H L P V N T L S G G N A Q R V S I A K W L A Query_1 1348 TTGACCATTAAGGCGCCGGATACGCATCTGCCGGTGAATACCCTCTCCGGTGGTAATGCCCAGCGCGTTTCTATCGCCAAATGGCTGGCG 1437 0 1147 .......................................................................................... 1236 0 1142 ......... 1134 2 400 .......................................................................................... 311 2 405 ......... 413 I S P K L L I L D S P T V G V D I A N K A G I Y H I I S D L Query_1 1438 ATCTCTCCTAAGCTGCTGATCCTCGATAGCCCAACCGTGGGGGTGGATATCGCAAACAAAGCCGGGATCTATCATATCATCAGCGATTTG 1527 0 1237 .......................................................................................... 1326 2 310 .......................................................................................... 221 A A H G I A V L M I C D E I E E A W Y Q S H R I L V M K Q G Query_1 1528 GCTGCCCATGGTATCGCCGTGCTGATGATTTGCGACGAGATTGAAGAGGCGTGGTACCAGAGTCATCGCATCCTGGTGATGAAGCAGGGC 1617 0 1327 .......................................................................................... 1416 0 1023 ........... 1033 2 220 .......................................................................................... 131 2 524 ........... 514 E L T H S F L P D S S T Q Q Q I A E V V N G * H W K W I T D Query_1 1618 GAGTTGACCCATAGCTTCCTGCCGGACAGCAGTACCCAGCAGCAGATAGCGGAGGTGGTCAATGGCTAACATTGGAAGTGGATAACGGAT 1707 0 1417 .................................................................. 1482 2 130 ......................................................................................--.. 43 P N S S S V D K L A A A L E H H H H H H * D P A A N K A R K Query_1 1708 CCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAG 1797 2 42 ... 40 2 44 .........................A 19 E Query_1 1798 GAAG 1801 2 18 .... 15 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 7513660 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 13, 2018 11:15 AM Number of letters in database: 4,069 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96932.102 (IDP96932.1-808.F..pMCSG68)
MPDINPQNIKELRALVEQQLQYTLCVSLNKATHGDIFNAVALAIRHFQQDHFLLSQKRQREERKKRVYYLSMEFLLGQSLRNNLLNMNLLAEMHQVVNDLGFDLDHLLDEEPDAALGNGGLGRLAACFIDSMATLDIAASGHGIKYEYGLFRQSFQNDQQIEHPDDWHSMHSPWLVEHHAQQILIPLGGYIEHSEDVDGNYNPMWLGWKTIIGIPHDYFVSGYRDTTTNKLRLYSAVASDSFNIHIFNRGDYIKAVSDKIASENISKILYPSDEVLTGKELRLTQEYFLVACTLRDIFRDYAEVNDDITFLPQHVAIQLNDTHPALAVVELMRILVDEYRLPWEQAWEITQNTCAYTNHTLMPEALETWPVTLFEKLLPRHLQIIFEINHRFLEEVARRWPDKNHLLSSLSLIDEHRDKQIRMANLAIVGSHRVNGVAWLHSELVKKQLVPDFYFMTPEKFINQTNGVTPRRWVQQANPGLAAFLHQQLGEGWPTDLPLLSSLESLADDTAVIDNIRAIKFANKTALSQLVAQRYGIALDPLAMFDCQVKRIHEYKRQLLNILHVIALYLDIKETGKTIAPKVHLFAGKAAPGYRMAKLIIQLINTVARKINRDPQVAGQLKVVFLEDYKVSLAEKIIPATDLSEQISTAGTEASGTSNMKFAMNGALTIGTYDGANIEIREAVGIDNFYLFGAVAEEIEESRRLGHHHNFYHQNPAMARVVDTLVSGLFTSDRELFAPLHHMLTEGDHYCHLLDFDSYCQAQRQAMADFELPEQWHRRALLNICRMGEFSSDRTIRGYARDIWGITS
ATGCCTGACATTAACCCGCAAAATATCAAAGAATTACGGGCGTTGGTTGAACAGCAATTACAGTACACATTGTGTGTTAGCTTAAATAAAGCTACGCATGGCGATATTTTTAACGCGGTGGCGCTGGCCATTCGTCATTTTCAACAGGACCATTTTTTGTTGAGCCAGAAGCGGCAACGTGAAGAACGTAAAAAGCGCGTTTACTATCTGTCGATGGAGTTTTTACTGGGACAGTCGCTGCGTAATAACCTTTTGAATATGAATTTACTGGCGGAGATGCACCAGGTGGTGAATGACCTGGGGTTTGATCTTGACCACCTTCTTGACGAAGAGCCCGACGCCGCGCTGGGTAACGGAGGGTTAGGGCGCTTAGCCGCCTGTTTTATCGATTCGATGGCCACCCTTGATATTGCCGCATCCGGGCATGGCATCAAATACGAATATGGTCTTTTTCGCCAATCCTTTCAAAATGACCAGCAGATCGAGCATCCGGATGACTGGCATTCCATGCACTCCCCGTGGCTGGTCGAACATCACGCTCAGCAAATCCTGATCCCGCTGGGAGGCTATATTGAACACAGCGAAGATGTTGATGGCAATTACAATCCAATGTGGCTGGGCTGGAAAACGATTATCGGTATACCCCATGACTATTTCGTTTCCGGTTACCGGGATACGACGACCAACAAGCTGCGGCTTTATAGCGCGGTGGCCTCTGACTCCTTTAATATCCATATTTTCAATCGCGGTGATTATATCAAGGCGGTGAGTGATAAAATCGCGTCGGAGAATATTTCAAAAATACTCTATCCATCCGATGAGGTGCTGACCGGAAAAGAGCTGCGGCTGACGCAAGAGTATTTTCTGGTGGCCTGTACGCTGCGGGATATATTTCGCGACTATGCCGAGGTTAATGACGATATTACTTTCCTGCCGCAGCATGTGGCGATTCAGCTGAATGACACCCATCCGGCATTAGCGGTGGTGGAGCTGATGCGTATCCTCGTCGATGAATACCGGCTGCCCTGGGAGCAGGCGTGGGAGATAACGCAAAACACCTGTGCCTACACGAATCATACGTTGATGCCGGAAGCGCTGGAGACCTGGCCGGTGACGCTGTTTGAAAAACTGCTGCCGCGTCATTTGCAAATTATATTTGAGATCAACCATCGTTTCCTTGAAGAGGTGGCCCGGCGCTGGCCGGATAAAAATCATCTGTTATCTTCGCTGTCTTTAATCGACGAACATCGCGATAAGCAAATCCGTATGGCCAATCTGGCGATTGTCGGTAGCCATCGCGTGAACGGGGTAGCATGGCTGCATTCGGAACTGGTCAAGAAACAACTGGTGCCCGACTTCTATTTTATGACCCCGGAGAAATTCATTAATCAGACCAACGGCGTCACGCCGCGACGCTGGGTGCAGCAGGCTAACCCCGGCCTGGCCGCTTTTTTGCACCAACAGCTGGGGGAAGGGTGGCCAACCGATTTACCCTTGCTGTCCAGTCTGGAAAGCCTGGCCGATGATACTGCTGTTATTGATAATATCCGGGCCATTAAATTTGCCAACAAAACCGCGCTCAGCCAGCTGGTCGCTCAACGCTATGGCATCGCCCTCGATCCGCTGGCGATGTTTGATTGTCAGGTCAAACGTATTCATGAATATAAGCGTCAGTTGCTCAATATTCTTCATGTGATCGCGCTTTATCTGGATATCAAAGAGACAGGGAAAACTATCGCGCCTAAGGTGCATTTGTTTGCGGGTAAAGCGGCGCCGGGCTATCGGATGGCGAAGCTGATTATTCAGTTGATTAATACCGTCGCCCGGAAAATTAACCGCGACCCGCAGGTTGCCGGGCAGCTTAAAGTGGTTTTCCTCGAAGATTATAAAGTCTCGCTGGCGGAAAAAATCATTCCGGCCACAGACCTCTCCGAGCAAATTTCCACCGCCGGGACCGAGGCGTCCGGCACCAGCAATATGAAGTTTGCCATGAACGGCGCGTTAACCATCGGCACCTATGATGGGGCGAATATCGAAATTCGCGAGGCCGTGGGGATCGATAATTTTTATCTGTTTGGCGCCGTGGCGGAAGAGATTGAAGAGAGCCGGCGCCTTGGGCATCACCATAATTTCTATCATCAGAACCCGGCTATGGCGCGTGTTGTCGATACCCTCGTGTCTGGGTTGTTCACCAGCGATCGTGAGCTGTTTGCACCCCTTCACCACATGTTGACTGAGGGCGATCATTACTGTCATTTACTGGATTTCGACAGTTATTGTCAGGCCCAGCGGCAGGCGATGGCCGATTTTGAACTGCCGGAACAATGGCACCGTCGGGCCTTACTCAATATTTGCCGGATGGGCGAATTTTCCAGCGACAGAACCATACGTGGCTACGCCCGGGATATTTGGGGTATCACCAGC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,713 total letters Query= IDP96932.102 Length=2829 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4372 0.0 2_11A_A3_IDP96932.102_T7term.seq 1764 0.0 1_07A_A3_IDP96932.102_T7S.seq 1651 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 58 TTCCCCT-CTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTG 146 1 6 .......N..N...-............................................---............................ 91 W S H P Q F E K G T E N L Y F Q S N A M P D I N P Q N I K E Query_1 147 GTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGCCTGACATTAACCCGCAAAATATCAAAGAATT 236 0 1 ................................... 35 1 92 .......................................................................................... 181 L R A L V E Q Q L Q Y T L C V S L N K A T H G D I F N A V A Query_1 237 ACGGGCGTTGGTTGAACAGCAATTACAGTACACATTGTGTGTTAGCTTAAATAAAGCTACGCATGGCGATATTTTTAACGCGGTGGCGCT 326 0 36 .......................................................................................... 125 1 182 .......................................................................................... 271 L A I R H F Q Q D H F L L S Q K R Q R E E R K K R V Y Y L S Query_1 327 GGCCATTCGTCATTTTCAACAGGACCATTTTTTGTTGAGCCAGAAGCGGCAACGTGAAGAACGTAAAAAGCGCGTTTACTATCTGTCGAT 416 0 126 .......................................................................................... 215 1 272 .......................................................................................... 361 M E F L L G Q S L R N N L L N M N L L A E M H Q V V N D L G Query_1 417 GGAGTTTTTACTGGGACAGTCGCTGCGTAATAACCTTTTGAATATGAATTTACTGGCGGAGATGCACCAGGTGGTGAATGACCTGGGGTT 506 0 216 .......................................................................................... 305 1 362 .......................................................................................... 451 F D L D H L L D E E P D A A L G N G G L G R L A A C F I D S Query_1 507 TGATCTTGACCACCTTCTTGACGAAGAGCCCGACGCCGCGCTGGGTAACGGAGGGTTAGGGCGCTTAGCCGCCTGTTTTATCGATTCGAT 596 0 306 .......................................................................................... 395 0 1482 . 1482 1 452 .......................................................................................... 541 M A T L D I A A S G H G I K Y E Y G L F R Q S F Q N D Q Q I Query_1 597 GGCCACCCTTGATATTGCCGCATCCGGGCATGGCATCAAATACGAATATGGTCTTTTTCGCCAATCCTTTCAAAATGACCAGCAGATCGA 686 0 396 .......................................................................................... 485 0 1481 .......... 1472 1 542 ....................................................................................T..... 631 E H P D D W H S M H S P W L V E H H A Q Q I L I P L G G Y I Query_1 687 GCATCCGGATGACTGGCATTCCATGCACTCCCCGTGGCTGGTCGAACATCACGCTCAGCAAATCCTGATCCCGCTGGGAGGCTATATTG- 775 0 486 .........................................................................................- 574 0 1238 ...A- 1235 1 632 .........................................................................................A 721 E H S E D V D G N Y N P M W L G W K T I I G I P H D Y F V S Query_1 776 AACACAGCGAAGATGTTGATGGCAA-TTACAATCCAATGTGGCTGGGCTGGAAAACGATTATCGGTATACCCCATGACTATTTCGTTTCC 864 0 575 .........................-................................................................ 663 0 1234 ..G........... 1221 1 722 .........................T....................................N........................... 811 G Y R D T T T N K L R L Y S A V A S D S F N I H I F N R G Query_1 865 GGTTACCGGGATACGACGACCAACAAGCTGCGGCTTTATAGCGCGGTGGCCT-CTGACTCCTTTAATATCCATA-TTTTCAATCGCGGTG 952 0 664 ....................................................-.....................-............... 751 1 812 ...........................................-........N.....NN....N....-....T............... 899 D Y I K A V S D K I A S E N I S K I L Y P S D E V L T G K Query_1 953 ATTATA-TCAAGGCGG-TGAGTGATAAAATCGCGTCGGAGAATATTTCAAAAATACTCTATCCATCCGATGAGGTGCTGACCGGAAAAGA 1040 0 752 ......-.........-......................................................................... 839 1 900 ......T.........T........-.......NGNN..A......--C..........N.-..................N...-..... 984 E L R L T Q E Y F L V A C T L R D I F R D Y A E V N D D I T Query_1 1041 GCTGCGGCTGACGCAAGAGTATTTTCTGGTGGCCTGTACG-CTGCGGGATATATTTCGCGACTATGCCGAGGTTAATGACGATATTACTT 1129 0 840 ........................................-................................................. 928 1 985 ..................-......TC...N.....G...C..N..... 1032 F L P Q H V A I Q L N D T H P A L A V V E L M R I L V D E Y Query_1 1130 TCCTGCCGCAGCATGTGGCGATTCAGCTGAATGACACCCATCCGGCATTAGCGGTGGTGGAGCTGATGCGTATCCTCGTCGATGAATACC 1219 0 929 .......................................................................................... 1018 0 960 ............ 949 0 2363 ........... 2353 R L P W E Q A W E I T Q N T C A Y T N H T L M P E A L E T W Query_1 1220 GGCTGCCCTGGGAGCAGGCGTGGGAGATAACGCAAAACACCTGTGCCTACACGAATCATACGTTGATGCCGGAAGCGCTGGAGACCTGGC 1309 0 1019 .......................................................................................... 1108 P V T L F E K L L P R H L Q I I F E I N H R F L E E V A R R Query_1 1310 CGGTGACGCTGTTTGAAAAACTGCTGCCGCGTCATTTGCAAATTATATTTGAGATCAACCATCGTTTCCTTGAAGAGGTGGCCCGGCGCT 1399 0 1109 .......................................................................................... 1198 W P D K N H L L S S L S L I D E H R D K Q I R M A N L A I V Query_1 1400 GGCCGGATAAAAATCATCTGTTATCTTCGCTGTCTTTAATCGACGAACATCGCGATAAGCAAATCCGTATGGCCAATCTGGCGATTGTCG 1489 0 1199 .......................................................................................... 1288 0 588 ...........G..C... 571 1 735 ...........G... 721 G S H R V N G V A W L H S E L V K K Q L V P D F Y F M T P E Query_1 1490 GTAGCCATCGCGTGAACGGGGTAGCATGGCTGCATTCGGAACTGGTCAAGAAACAACTGGTGCCCGACTTCTATTTTATGACCCCGGAGA 1579 0 1289 .......................................................................................... 1378 2 1184 ...........N... 1170 K F I N Q T N G V T P R R W V Q Q A N P G L A A F L H Query_1 1580 AATTCATTAATCAGA-CCAAC--GGCGTCACG-CCGCGACGCTGG-GTG-CAGC-AGGCTAACCCCGGCCTGGCCGC--TTTTTTGCA-C 1659 0 1379 ...............-.....--.........-............-...-....-......................--.........-. 1458 2 1169 .T.NN--........T.....CG..N......G........N...A...G....C.N.....N.....G.NT.A..NNT.........N. 1082 Q Q L G E G W P T D L P L L S S L E S L A D D T A V I D N Query_1 1660 CAACAGCTGGGGGAA-GGGTGG-CCAACCGA-TTTACCCTTGCTGTCCAGTCTGGAAAGCCTGGCCGATGATACTGCTGTTATTGATAAT 1746 0 1459 ...............-......-........-.......................................................... 1545 0 405 ..-......-... 395 2 1081 .........N.....A......A........A.......N..N-....................................-......... 994 1 551 ..-......-... 541 I R A I K F A N K T A L S Q L V A Q R Y G I A L D P L A M F Query_1 1747 ATCCGGGCCATTAAATTTGCCAACAAAACCGCGCTCAGCCAGCTGGTCGCTCAACGCTATGGCATCGCCCTCGATCCGCTGGCGATGTTT 1836 0 1546 .......................................................................................... 1635 2 993 ........A--...........-.................................N................................. 907 D C Q V K R I H E Y K R Q L L N I L H V I A L Y L D I K E T Query_1 1837 GATTGTCAGGTCAAACGTATTCATGAATATAAGCGTCAGTTGCTCAATATTCTTCATGTGATCGCGCTTTATCTGGATATCAAAGAGACA 1926 0 1636 .......................................................................................... 1725 0 1664 ............ 1653 2 906 .......................................................................................... 817 2 878 ............ 889 G K T I A P K V H L F A G K A A P G Y R M A K L I I Q L I N Query_1 1927 GGGAAAACTATCGCGCCTAAGGTGCATTTGTTTGCGGGTAAAGCGGCGCCGGGCTATCGGATGGCGAAGCTGATTATTCAGTTGATTAAT 2016 0 1726 .......................................................................................... 1815 2 816 ....................................N..................................................... 727 T V A R K I N R D P Q V A G Q L K V V F L E D Y K V S L A E Query_1 2017 ACCGTCGCCCGGAAAATTAACCGCGACCCGCAGGTTGCCGGGCAGCTTAAAGTGGTTTTCCTCGAAGATTATAAAGTCTCGCTGGCGGAA 2106 0 1816 .......................................................................................... 1905 2 726 .......................................................................................... 637 K I I P A T D L S E Q I S T A G T E A S G T S N M K F A M N Query_1 2107 AAAATCATTCCGGCCACAGACCTCTCCGAGCAAATTTCCACCGCCGGGACCGAGGCGTCCGGCACCAGCAATATGAAGTTTGCCATGAAC 2196 0 1906 .......................................................................................... 1995 2 636 .......................................................................................... 547 G A L T I G T Y D G A N I E I R E A V G I D N F Y L F G A V Query_1 2197 GGCGCGTTAACCATCGGCACCTATGATGGGGCGAATATCGAAATTCGCGAGGCCGTGGGGATCGATAATTTTTATCTGTTTGGCGCCGTG 2286 0 1996 .......................................................................................... 2085 2 546 .......................................................................................... 457 A E E I E E S R R L G H H H N F Y H Q N P A M A R V V D T L Query_1 2287 GCGGAAGAGATTGAAGAGAGCCGGCGCCTTGGGCATCACCATAATTTCTATCATCAGAACCCGGCTATGGCGCGTGTTGTCGATACCCTC 2376 0 2086 .......................................................................................... 2175 2 456 .......................................................................................... 367 V S G L F T S D R E L F A P L H H M L T E G D H Y C H L L D Query_1 2377 GTGTCTGGGTTGTTCACCAGCGATCGTGAGCTGTTTGCACCCCTTCACCACATGTTGACTGAGGGCGATCATTACTGTCATTTACTGGAT 2466 0 2176 .......................................................................................... 2265 2 366 .......................................................................................... 277 F D S Y C Q A Q R Q A M A D F E L P E Q W H R R A L L N I C Query_1 2467 TTCGACAGTTATTGTCAGGCCCAGCGGCAGGCGATGGCCGATTTTGAACTGCCGGAACAATGGCACCGTCGGGCCTTACTCAATATTTGC 2556 0 2266 .......................................................................................... 2355 0 975 ... 973 2 276 ..............................N.......N....N.....-C.........N................-............ 189 R M G E F S S D R T I R G Y A R D I W G I T S * H W K W I T Query_1 2557 CGGATGGGCGAATTTTCCAGCGACAGAACCATACGTGGCTACGCCCGGGATATTTGGGGTATCACCAGCTAACATTGGAAGTGGATAACG 2646 0 2356 ..................................................................... 2424 0 972 ........ 965 2 188 .......C.......C...C...............N....N.....C.....C..........N..........C........N.-CC.. 100 D P N S S S V D K L A A A L E H H H H H H * D P A A N K A R Query_1 2647 GATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGA 2736 2 99 .....CC..N.......NC..........N...C........N......-N...........N........N.NC....N.....N.... 11 K Query_1 2737 AAG 2739 2 10 ... 8 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 13109544 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 13, 2018 11:15 AM Number of letters in database: 4,713 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96934.102 (IDP96934.21-344.P(344)..pMCSG68)
SVSARMTCSINVDMKYVKPPISRIKDDTGNLRPPSPMQQVHTESMLAGQDIYIQSIKSEKDLRLMLVSAIAWKNGLGNEWFEASKEYLLAWINTYEPEYHPINETVFDQLIQTYVIIKSNLSPQERSDINKYLYSWANGYITRMNNAPKGISWYSNWQSHRIKLITLIAVATDNDHLFNESRRLFKKQIEENMNSEGEVVDFKQRDAIHYVVYDLQPLLQAALAAMSRGEDWYHWTAPNNASLEKGINWLTPYVTGEKTHNEFINSKIKLDAIRKQRGLKNFNGIFRPEEAKSVYWLASAFNASYEPLALKLLAKPPLIMDWCN
TCCGTATCAGCACGGATGACATGTTCTATCAATGTCGACATGAAATACGTCAAGCCTCCAATTTCAAGAATTAAAGATGATACAGGCAATCTTCGTCCTCCATCACCGATGCAGCAGGTTCATACGGAAAGCATGTTAGCTGGGCAAGATATTTATATTCAAAGCATTAAATCGGAAAAAGATCTGCGCCTAATGTTAGTAAGTGCGATCGCATGGAAAAATGGATTGGGAAATGAGTGGTTTGAAGCATCGAAAGAATATCTTCTAGCTTGGATTAACACTTATGAGCCGGAATATCACCCCATAAATGAAACTGTTTTTGATCAATTAATTCAAACGTACGTAATTATTAAGTCAAATCTATCACCCCAAGAGCGCAGCGATATAAACAAATATTTATATAGTTGGGCAAATGGATACATAACTCGAATGAATAATGCACCAAAAGGAATATCTTGGTATAGTAACTGGCAAAGCCACAGAATTAAACTCATTACATTAATAGCTGTTGCAACAGATAATGATCACCTTTTTAACGAATCAAGAAGATTATTTAAAAAGCAAATTGAAGAAAACATGAATAGTGAGGGGGAGGTTGTTGACTTCAAACAACGAGATGCTATTCATTATGTTGTATACGACCTCCAGCCGCTACTACAGGCTGCACTTGCCGCAATGTCACGCGGTGAAGATTGGTACCATTGGACTGCTCCAAATAATGCGTCACTTGAAAAAGGAATAAACTGGCTGACCCCCTATGTCACAGGAGAAAAAACGCATAATGAATTTATAAATTCAAAAATAAAACTTGACGCCATAAGAAAGCAGCGCGGTCTAAAAAATTTCAATGGTATATTTCGGCCTGAGGAAGCTAAATCAGTTTACTGGTTAGCATCTGCATTCAATGCATCATATGAGCCACTAGCCTTGAAGCTCTTGGCTAAACCTCCTTTGATTATGGATTGGTGCAAT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,894 total letters Query= IDP96934.102 Length=1377 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1754 0.0 1_06C_C2_IDP96934.102_T7S.seq 205 1e-55 2_10C_C2_IDP96934.102_T7term.seq 134 1e-34 F P S R N N F V * L * E G D I H M H H H H H H S S G V D Query_1 58 TTCCCCTCTAGAAATAATTTTG-TTTAACTTTAAG-AAGGAGATATACATA-TGCACCA-TCATCATCATCATTCTTCTGGTGTAGATCT 143 1 1 .........NT..-..N.....N............C...............A.......G...........C.................. 89 L W S H P Q F E K G T E N L Y F Q S N A S V S A R M T C S I Query_1 144 -GTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCTCCGTATCAGCACGGATGACATGTTCTATCA 232 0 1 ............................... 31 1 90 T.......................................................... 148 N V D M K Y V K P P I S R I K D D T G N L R P P S P M Q Q V Query_1 233 ATGTCGACATGAAATACGTCAAGCCTCCAATTTCAAGAATTAAAGATGATACAGGCAATCTTCGTCCTCCATCACCGATGCAGCAGGTTC 322 0 32 .......................................................................................... 121 H T E S M L A G Q D I Y I Q S I K S E K D L R L M L V S A I Query_1 323 ATACGGAAAGCATGTTAGCTGGGCAAGATATTTATATTCAAAGCATTAAATCGGAAAAAGATCTGCGCCTAATGTTAGTAAGTGCGATCG 412 0 122 .......................................................................................... 211 A W K N G L G N E W F E A S K E Y L L A W I N T Y E P E Y H Query_1 413 CATGGAAAAATGGATTGGGAAATGAGTGGTTTGAAGCATCGAAAGAATATCTTCTAGCTTGGATTAACACTTATGAGCCGGAATATCACC 502 0 212 .......................................................................................... 301 P I N E T V F D Q L I Q T Y V I I K S N L S P Q E R S D I N Query_1 503 CCATAAATGAAACTGTTTTTGATCAATTAATTCAAACGTACGTAATTATTAAGTCAAATCTATCACCCCAAGAGCGCAGCGATATAAACA 592 0 302 .......................................................................................... 391 K Y L Y S W A N G Y I T R M N N A P K G I S W Y S N W Q S H Query_1 593 AATATTTATATAGTTGGGCAAATGGATACATAACTCGAATGAATAATGCACCAAAAGGAATATCTTGGTATAGTAACTGGCAAAGCCACA 682 0 392 .......................................................................................... 481 1 48 ........... 58 R I K L I T L I A V A T D N D H L F N E S R R L F K K Q I E Query_1 683 GAATTAAACTCATTACATTAATAGCTGTTGCAACAGATAATGATCACCTTTTTAACGAATCAAGAAGATTATTTAAAAAGCAAATTGAAG 772 0 482 .......................................................................................... 571 0 520 ..T............C.. 503 1 451 ........... 461 E N M N S E G E V V D F K Q R D A I H Y V V Y D L Q P L L Q Query_1 773 AAAACATGAATAGTGAGGGGGAGGTTGTTGACTTCAAACAACGAGATGCTATTCATTATGTTGTATACGACCTCCAGCCGCTACTACAGG 862 0 572 .......................................................................................... 661 A A L A A M S R G E D W Y H W T A P N N A S L E K G I N W L Query_1 863 CTGCACTTGCCGCAATGTCACGCGGTGAAGATTGGTACCATTGGACTGCTCCAAATAATGCGTCACTTGAAAAAGGAATAAACTGGCTGA 952 0 662 .......................................................................................... 751 T P Y V T G E K T H N E F I N S K I K L D A I R K Q R G L K Query_1 953 CCCCCTATGTCACAGGAGAAAAAACGCATAATGAATTTATAAATTCAAAAATAAAACTTGACGCCATAAGAAAGCAGCGCGGTCTAAAAA 1042 0 752 .......................................................................................... 841 0 798 ................ 783 N F N G I F R P E E A K S V Y W L A S A F N A S Y E P L A L Query_1 1043 ATTTCAATGGTATATTTCGGCCTGAGGAAGCTAAATCAGTTTACTGGTTAGCATCTGCATTCAATGCATCATATGAGCCACTAGCCTTGA 1132 0 842 .......................................................................................... 931 K L L A K P P L I M D W C N * H W K W I T D P N S S S V D Query_1 1133 AGCTCTTGGCTAAACCTCCTTTGATTATGGATTGGTGCAATTAACATTGGAAGTGGATAA-CGGATCCGAATTCGAGCTCCGTCGAC--A 1219 0 932 ......................................... 972 2 127 ...................T.........................TCA. 79 K L A A A L E H H H H H H * D P A A N K A R Query_1 1220 AGCTTGCGGCCGCA-CTCGAGcaccaccaccaccacca-cTGAGATCCGGCTGCTAACAAAGCCCGAA 1285 2 78 .AN.G.....N...N..........N........N..CT...................C......N.. 11 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5234406 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 13, 2018 11:15 AM Number of letters in database: 3,894 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96940.103 (IDP96940.1-329.F..pMCSG73)
MKDYGRNELCQVIISSHSSSILSRIEPEQIRHFRLDQISKSTVVSALTLPEKEDELSKFIKEAVKSYPEIYFSKLVIFGEGDSEEIIIPKVLELYSTEIDSHSISVVPLGGRHVNHFWRLLKSLQIPFITLLDFDIDRNGGGFGRLRYAIEQLSIFCGGKYGKTKEKIPAWDDERDPTTFKLPRTDGDSYNVVAALENHNIYFSSPLDIDYAMIEAFPDIFCEKDVVYGERGPKNIEQEEQEEQKKELINAVLKKGNSGVRYEFSVDYLKYFLWYRYRFLSNKSKPASHVRMFSKIEEKYTPDEIREKLPPVLIRVADKTVSLLAEVIE
GTGAAAGATTATGGTCGTAATGAGCTGTGCCAAGTTATTATTTCTTCCCATTCATCTTCCATTTTAAGCCGAATCGAGCCCGAGCAAATACGTCACTTTAGATTGGATCAGATATCAAAAAGCACGGTAGTAAGCGCATTAACGCTACCGGAAAAAGAAGACGAGCTTTCTAAGTTTATTAAAGAGGCAGTTAAATCATACCCTGAAATCTATTTTTCTAAACTGGTGATATTTGGCGAAGGGGATAGTGAGGAAATTATAATTCCAAAGGTGCTTGAGCTGTATTCGACGGAGATTGATAGCCATTCTATCTCTGTTGTTCCTCTTGGTGGACGTCATGTGAATCATTTTTGGCGTCTGTTGAAGTCATTACAGATTCCCTTTATCACTTTACTGGATTTCGATATCGATAGAAATGGCGGTGGGTTCGGCCGACTTAGATATGCTATTGAGCAACTGTCTATTTTCTGTGGTGGAAAATATGGCAAGACAAAAGAAAAAATACCTGCATGGGATGACGAACGAGATCCAACAACATTTAAACTTCCGCGTACTGACGGTGATTCATATAATGTTGTGGCTGCGTTGGAGAATCATAATATTTATTTTTCATCGCCTCTAGATATCGATTACGCAATGATTGAAGCATTCCCTGATATTTTCTGTGAAAAAGATGTGGTCTATGGGGAAAGAGGGCCTAAAAATATTGAACAGGAAGAACAGGAAGAACAAAAAAAAGAGCTGATAAATGCAGTATTGAAAAAAGGCAACTCCGGCGTCCGTTACGAATTTTCTGTTGATTATCTAAAATATTTCCTGTGGTATCGCTATCGCTTTTTATCCAATAAAAGTAAGCCAGCCTCTCATGTTAGAATGTTTTCTAAAATTGAAGAAAAATATACGCCGGATGAAATCAGAGAAAAATTGCCTCCAGTGTTAATTCGGGTAGCTGACAAGACTGTATCGTTATTGGCGGAGGTTATTGAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,521 total letters Query= IDP96940.103 Length=1392 Score E Sequences producing significant alignments: (Bits) Value 2_09B_B1_IDP96940.103_T7_Term.seq 1781 0.0 0_wt 1781 0.0 1_05B_B1_IDP96940.103_v73F.seq 1737 0.0 H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S N Query_1 109 CACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAAT 198 2 1102 ....N...N..N.........G.A..AG--..-.C................... 1052 2 1117 .............. 1104 1 41 ...........A........G....------....................... 88 1 25 ...C........... 39 1 26 .............. 39 A V K D Y G R N E L C Q V I I S S H S S S I L S R I E P E Query_1 199 GCCGTGAAAG--ATTATGGTCGTAATGAGCTGTGCCAAGTTATTATTTCTTCCCATTCATCTTCCATTTTAAGCCGAATCGAGCCCGAGC 286 2 1051 ...A.N...AGA......................G...................NA.............-.................... 963 0 1 .......--.............................................................................. 85 1 89 ...A......--.............................................................................. 176 Q I R H F R L D Q I S K S T V V S A L T L P E K E D E L S K Query_1 287 AAATACGTCACTTTAGATTGGATCAGATATCAAAAAGCACGGTAGTAAGCGCATTAACGCTACCGGAAAAAGAA-GACGAGCTTTCTAAG 375 2 962 ..................N............................................N.N........A............... 873 0 86 ..........................................................................-............... 174 1 177 ..........................................................................-............... 265 F I K E A V K S Y P E I Y F S K L V I F G E G D S E E I I Query_1 376 TTTATTAAAGAGGCAGTTAAATCATACCCTGAAATCTATTTTTCTAAACTGGTGATATTTGGCGAAGGGGATAGTGAGGAAATTATAA-T 464 2 872 ............................-...........................................................A. 784 0 175 ........................................................................................-. 263 1 266 ........................................................................................-. 354 I P K V L E L Y S T E I D S H S I S V V P L G G R H V N H F Query_1 465 TCCAAAGGTGCTTGAGCTGTATTCGACGGAGATTGATAGCCATTCTATCTCTGTTGTTCCTCTTGGTGGACGTCATGTGAATCATTTTTG 554 2 783 .......................................................................................... 694 0 264 .......................................................................................... 353 1 355 .......................................................................................... 444 W R L L K S L Q I P F I T L L D F D I D R N G G G F G R L R Query_1 555 GCGTCTGTTGAAGTCATTACAGATTCCCTTTATCACTTTACTGGATTTCGATATCGATAGAAATGGCGGTGGGTTCGGCCGACTTAGATA 644 2 693 ..................................................G....................................... 604 0 354 .......................................................................................... 443 1 445 ..................................................G....................................... 534 Y A I E Q L S I F C G G K Y G K T K E K I P A W D D E R D P Query_1 645 TGCTATTGAGCAACTGTCTATTTTCTGTGGTGGAAAATATGGCAAGACAAAAGAAAAAATACCTGCATGGGATGACGAACGAGATCCAAC 734 2 603 .......................................................................................... 514 2 390 ........... 380 0 444 .......................................................................................... 533 0 657 ........... 667 1 535 .......................................................................................... 624 1 748 ........... 758 T T F K L P R T D G D S Y N V V A A L E N H N I Y F S S P L Query_1 735 AACATTTAAACTTCCGCGTACTGACGGTGATTCATATAATGTTGTGGCTGCGTTGGAGAATCATAATATTTATTTTTCATCGCCTCTAGA 824 2 513 .......................................................................................... 424 0 534 .......................................................................................... 623 1 625 .......................................................................................... 714 D I D Y A M I E A F P D I F C E K D V V Y G E R G P K N I E Query_1 825 TATCGATTACGCAATGATTGAAGCATTCCCTGATATTTTCTGTGAAAAAGATGTGGTCTATGGGGAAAGAGGGCCTAAAAATATTGAACA 914 2 423 .......................................................................................... 334 2 585 ........... 575 0 624 .......................................................................................... 713 0 462 ........... 472 1 715 .......................................................................................... 804 1 553 ........... 563 Q E E Q E E Q K K E L I N A V L K K G N S G V R Y E F S V D Query_1 915 GGAAGAACAGGAAGAAC-aaaaaaaaGAGCTGATAAATGCAGTATTGAAAAAAGGCAACTCCGGCGTCCGTTACGAATTTT-CTGTTGAT 1002 2 333 .................-..............................-................................-........ 247 0 714 .................-...............................................................-........ 801 1 805 .................A.......................N.....-.................................N........ 893 Y L K Y F L W Y R Y R F L S N K S K P A S H V R M F S K Query_1 1003 TATCTAAAATATTT-CCTGTGGTATCGCTA-TCGCTTTTTATCCAATAAAAGTAAGCCAGCCT--CTCATGTTAGAATGTTTTCTAAAAT 1088 2 246 ..............-...............-................................--......................... 161 0 802 ..............-...............-................................--......................... 887 1 894 ..............N...............T......................-.........TCT................CT...... 982 I E E K Y T P D E I R E K L P P V L I R V A D K T V S L L A Query_1 1089 TGAAGAAAAATATACGCCGGATGAAATCAGAGAAAAATTGCCTCCAGTGTTAATTCGGGTAGCTGACAAGACTGTATCGTTATTGGCGGA 1178 2 160 .......................................................................................... 71 0 888 .......................................................................................... 977 1 983 ................................-..................................-.....-NN........-.N... 1068 E V I E * H W K W I T D P N S S S V D K L A A A L E H H H H Query_1 1179 GGTTATTGAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccacca 1268 2 70 ..............................--..... 36 0 978 .......... 987 1 1069 .........T....... 1085 H H * D P A A N K A R K E A E L A A A T A E Q * L A * P L G Query_1 1269 ccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGC 1358 2 40 .........................A... 12 1 1115 ..N..........--.N....N......TCN....--T.........--.......--- 1164 A S K R V L R G F L L Query_1 1359 CTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAA 1391 1 1165 -NNNN...-....-...-...N........... 1193 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4778848 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Feb 13, 2018 11:15 AM Number of letters in database: 3,521 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2