Clone ID: CPX300513.102 (CPX300513.1-300.F..pMCSG68)
MSVLVNKDTKVIVQGITGSQGLFHTKQMIEYGTKIVGGVTPGKGGTDIEGVPVFDTVEDAVKATGANASVVYVPPAFAADAIMEAVDAEIDLVVCITEGIPVLDMVNVKRYMAGKHTRLLGPNCPGVITPDECKIGIMPGYIHKKGHVGIVSRSGTLTYEAVHQLTQEGIGQSTAVGIGGDPVNGTDFIDALKAFNEDEETHAVIMIGEIGGTAEEEAAEWVKANMTKPVVGFIGGQTAPAGKRMGHAGAIISGGKGTAAEKIKTMEACGIKVAETPAVMGETLISVLKEKGLFETCKNY
ATGAGCGTATTAGTTAATAAAGATACAAAAGTTATTGTTCAAGGTATTACAGGTTCTCAAGGATTATTCCACACAAAACAAATGATTGAATACGGTACGAAAATTGTCGGTGGTGTAACGCCAGGTAAAGGTGGCACTGACATTGAAGGTGTACCAGTATTTGATACTGTAGAAGATGCAGTGAAAGCAACAGGCGCAAACGCTTCAGTTGTATACGTTCCACCCGCTTTTGCGGCTGATGCAATTATGGAAGCAGTTGATGCAGAAATTGATTTAGTAGTATGTATTACCGAAGGAATTCCTGTATTAGATATGGTAAACGTAAAGAGATATATGGCTGGTAAACATACACGTTTACTTGGACCAAACTGCCCAGGTGTTATTACGCCTGATGAATGTAAAATCGGTATTATGCCAGGATACATTCATAAAAAAGGTCATGTAGGTATCGTATCTCGCTCTGGTACATTAACGTATGAGGCTGTACATCAGTTAACACAAGAAGGTATTGGCCAATCTACTGCTGTAGGTATCGGCGGAGACCCAGTTAACGGTACAGACTTTATTGATGCATTAAAAGCATTTAATGAAGATGAAGAAACACATGCTGTAATTATGATTGGTGAAATCGGTGGTACAGCGGAAGAAGAGGCAGCTGAATGGGTGAAAGCTAATATGACAAAACCTGTTGTAGGCTTCATTGGTGGCCAAACAGCGCCTGCTGGCAAACGTATGGGCCATGCTGGTGCAATCATTTCTGGCGGTAAAGGAACTGCTGCAGAAAAGATTAAAACGATGGAAGCTTGTGGTATTAAAGTAGCGGAAACTCCAGCAGTTATGGGAGAAACGCTAATCTCTGTCTTAAAAGAAAAAGGTCTATTTGAGACTTGTAAAAACTAC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,098 total letters Query= CPX300513.102 Length=2486 Score E Sequences producing significant alignments: (Bits) Value 2_12G_G9_CPX300513.102_T7term.seq 1644 0.0 0_wt 1624 0.0 1_09G_G9_CPX300513.102_T7S.seq 1454 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 60 CCCCTC-TAGAAATAATTTTGTTTAACTTTAAGAAGGAGA-TATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGG 147 1 12 ...N..N.................................N................................................. 101 S H P Q F E K G T E N L Y F Q S N A M N I H E Y Q G K A V L Query_1 148 TCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGAATATCCATGAGTACCAAGGTAAGGCAGTCCTT 237 2 965 ........... 975 0 58 ........... 48 1 102 .......................................................... 159 1 213 ........... 203 R S Y G V S V P N G K V A F T V E E A V E A A K E L G T D V Query_1 238 AGAAGTTATGGGGTTAGCGTTCCGAACGGGAAGGTTGCATTTACAGTAGAAGAAGCTGTAGAAGCAGCGAAAGAATTAGGAACGGATGTA 327 C V V K A Q I H A G G R G K A G G V K V A K N L D E V R T Y Query_1 328 TGTGTAGTAAAAGCACAAATTCACGCTGGTGGACGCGGCAAAGCTGGCGGAGTAAAAGTTGCAAAAAATTTAGATGAAGTTCGTACATAT 417 A E S I L G T T L V T H Q T G P E G K E V K R L L I E E G C Query_1 418 GCAGAGAGCATTTTAGGAACTACACTTGTGACACATCAAACAGGTCCTGAAGGTAAGGAAGTAAAACGCTTACTTATCGAAGAAGGTTGC 507 D I K K E Y Y V G L V L D R A T S Q V V L M A S E E G G T E Query_1 508 GATATTAAAAAAGAATATTATGTAGGTCTTGTTTTAGATCGTGCAACTTCTCAAGTTGTTTTAATGGCGTCTGAAGAAGGCGGAACAGAA 597 I E E V A E K T P E K I F K E Y I D P A V G L Q G F Q A R R Query_1 598 ATTGAAGAAGTAGCAGAAAAAACACCAGAAAAAATCTTTAAAGAATATATTGATCCAGCAGTAGGTTTACAAGGCTTCCAAGCACGCCGG 687 I A F N I N I P K E L V G Q A V K F M M G L Y R A F I E K D Query_1 688 ATTGCATTTAACATCAATATTCCAAAAGAGCTTGTAGGACAAGCTGTGAAGTTTATGATGGGCTTATACCGTGCGTTTATCGAAAAAGAT 777 C S I A E I N P L V T T G D G K V M A L D A K L N F D S N A Query_1 778 TGTTCTATCGCAGAGATTAATCCACTTGTTACAACAGGTGACGGTAAAGTTATGGCATTAGATGCGAAATTAAACTTTGATTCTAACGCT 867 L Y R H K D I L E L R D L D E E D A K E I E A S K Y D L N Y Query_1 868 TTATATCGCCATAAAGACATTTTAGAACTTCGTGATCTTGATGAAGAAGATGCAAAAGAAATCGAAGCTTCTAAATATGACTTAAACTAC 957 I P L D G N I G C M V N G A G L A M A T M D I I K H Y H G D Query_1 958 ATTCCTTTAGATGGAAATATCGGTTGTATGGTTAATGGTGCAGGTTTAGCGATGGCTACGATGGATATCATTAAACATTACCATGGCGAT 1047 P A N F L D V G G G A T A E K V T E A F K I I L S D K N V K Query_1 1048 CCAGCTAACTTCTTAGATGTTGGTGGCGGTGCGACAGCTGAAAAAGTTACAGAAGCATTTAAAATTATCCTTTCTGACAAAAATGTAAAA 1137 G I F V N I F G G I M K C D V I A E G V I E A T K Q V G L E Query_1 1138 GGTATCTTCGTTAACATTTTTGGTGGCATTATGAAGTGTGATGTTATTGCAGAAGGTGTTATTGAAGCAACGAAACAAGTAGGTCTTGAA 1227 L P L V V R L E G T N V E L G K K I L N E S G L N I V A A E Query_1 1228 TTGCCTTTAGTTGTACGTCTTGAAGGTACAAACGTTGAGTTAGGTAAGAAAATTTTAAATGAGTCTGGCTTAAATATTGTTGCAGCAGAA 1317 S M T D G A Q K I V S L V G * * K A G E K K * A Y * L I K I Query_1 1318 TCTATGACAGACGGTGCACAAAAAATTGTTTCACTAGTGGGCTAATAGAAAGCGGGGGAGAAAAAATGAGCGTATTAGTTAATAAAGATA 1407 2 1015 ...........-.......... 995 0 1 ......................... 25 1 156 ......................... 180 Q K L L F K V L Q V L K D Y S T Q N K * L N T V R K L S V V Query_1 1408 CAAAAGTTATTGTTCAAGGTATTACAGGTTCTCAAGGATTATTCCACACAAAACAAATGATTGAATACGGTACGAAAATTGTCGGTGGTG 1497 2 994 ....-.....-...........-............N..N.N...NC..---.....-.........N..-..G--.......TC...... 915 0 26 .......................................................................................... 115 1 181 .......................................................................................... 270 1 139 ........... 129 * R Q V K V A L T L K V Y Q Y L I L * K M Q * K Q Q A Q T L Query_1 1498 TAACGCCAGGTAAAGGTGGCACTGACATTGAAGGTGTACCAGTATTTGATACTGTAGAAGATGCAGTGAAAGCAACAGGCGCAAACGCTT 1587 2 914 ....-.............-..NN........................................................N.......... 827 0 116 .......................................................................................... 205 1 271 .................................................................N........................ 360 Q L Y T F H P L L R L M Q L W K Q L M Q K L I * * Y V L P Query_1 1588 CAGTTGTATACGTTCCACCCGCTTTTGCGGCTGATGC-AATTATGGAAGCAGTTGATGCAGAAATTGATTTAGTAGTATGTATTACCGAA 1676 2 826 ..............................T......N.................................................... 737 0 206 .....................................-.................................................... 294 1 361 .....................................-.................................................... 449 K E F L Y * I W * T * R D I W L V N I H V Y L D Q T A Q V L Query_1 1677 GGAATTCCTGTATT-AGATATGGTAAACGTAAAGAGATATATGGCTGG-TAAACATACACGTTTACTTGGACCAAACTGCCCAGGTGTTA 1764 2 736 ..............N.................................G..................N...................... 647 0 295 ..............-.................................-......................................... 382 1 450 ..............-.................................-......................................... 537 L R L M N V K S V L C Q D T F I K K V M * V S Y L A L V H * Query_1 1765 TTACGCCTGATGAATGTAAAATCGGTATTATGCCAGGATACATTCATAAAAAAGGTCATGTAGGTATCGTATCTCGCTCTGGTACATTAA 1854 2 646 .......................................................................................... 557 2 504 ........... 494 0 383 .......................................................................................... 472 0 525 ........... 535 1 538 ...........................................................................T.............. 627 1 680 ........... 690 R M R L Y I S * H K K V L A N L L L * V S A E T Q L T V Q T Query_1 1855 CGTATGAGGCTGTACATCAGTTAACACAAGAAGGTATTGGCCAATCTACTGCTGTAGGTATCGGCGGAGACCCAGTTAACGGTACAGACT 1944 2 556 .......................................................................................... 467 2 588 ........... 578 0 473 .......................................................................................... 562 0 441 ........... 451 1 628 ......................N........N.......................................................... 717 1 596 ........... 606 L L M H * K H L M K M K K H M L * L * L V K S V V Q R K K Query_1 1945 TTATTGATGCATTAAAAGCATTTAATGAAGATGAAGAAACACATGCTGTAATTATGA-TTGGTGAAA-TCGGTGGTACAGCGGAAG-AAG 2031 2 466 .........................................................-.........-..................-... 380 0 563 .........................................................-.........-..................-... 649 1 718 ...................................N.....................T....N....T.......N..........A... 807 R Q L N G * K L I * Q N L L * A S L V A K Q R L L A N V W Query_1 2032 AGGCAGCTGAATGGGTGAAAGCTAATATG-ACAAAACCTGTTGTAGGCTTCATTGGTGGCCAAACAGCGCCTGCTGGCAAACGTATGGGC 2120 2 379 .............................-............................................................ 291 0 650 .............................-............................................................ 738 1 808 .....-.........N.............A.....N.......................-........N....N........-....... 894 A M L V Q S F L A V K E L L Q K R L K R W K L V V L K * R K Query_1 2121 CATGCTGG-TGCAATCATTTCTGGCGGTAAAGGAACTGCTGCAGAAAAGATTAAAACGATGGAAGCTTGTGGTATTAAAGTAGCGG-AAA 2208 2 290 ........-.............................................................................-... 203 0 739 ........-.............................................................................-... 826 1 895 ......N.T.......T.............N............-.............N........-.............-.N...A... 981 1 917 ........... 907 L Q Q L W E K R * S L S * K K K V Y L R L V K T T N I G S Query_1 2209 CTCCAGCAGTTATGGGAGAAACGCTAATCT-CTGTCTTAAAAGAAAAAGGTCTATTTGAGACTTGTAAAAACTACTAACATTGGAAGTGG 2297 2 202 ..............................-........................................................... 114 0 827 ..............................-............................................ 900 1 982 ....................N....C....C...N......--G...G.....N.....A..-......N....NCT.......T..... 1068 G * R I R I R A P S T S L R P H S S T T T T T T E I R L L T Query_1 2298 ATAACGGATCCG-AATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAA 2386 2 113 ............-.......................................................................-N.... 26 1 1069 .....-.....NA........T....NN...T.. 1101 K P E R Query_1 2387 AGCCCGAAAGGAA 2399 2 25 .....N...A... 13 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 7523043 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 3,098 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP04388.104 (IDP04388.1-334.F..pMCSG68)
MITSSLPLTDLHRHLDGNIRTQTILELGQKFGVKLPANTLQTLTPYVQIVEAEPSLVAFLSKLDWGVAVLGDLDACRRVAYENVEDALNARIDYAELRFSPYYMAMKHSLPVTGVVEAVVDGVRAGVRDFGIQANLIGIMSRTFGTDACQQELDAILSQKNHIVAVDLAGDELGQPGDRFIQHFKQVRDAGLHVTVHAGEAAGPESMWQAIRDLGATRIGHGVKAIHDPKLMDYLAQHRIGIESCLTSNLQTSTVDSLATHPLKRFLEHGILACINTDDPAVEGIELPYEYEVAAPQAGLSQEQIRQAQLNGLELAFLSDSEKKALLAKAALRG
ATGATAACTTCATCACTCCCTTTAACGGATTTGCACCGTCATCTTGATGGAAATATCCGCACCCAAACCATTTTGGAATTAGGACAAAAATTTGGCGTTAAGCTACCCGCAAACACGTTACAAACTCTTACCCCTTATGTACAAATTGTCGAAGCAGAACCCTCTCTGGTTGCCTTCTTATCCAAACTAGACTGGGGCGTAGCGGTACTAGGCGATCTTGATGCTTGCCGTCGTGTTGCCTACGAGAACGTCGAAGATGCGCTTAACGCCCGTATTGACTATGCCGAGCTACGTTTTTCTCCTTATTACATGGCCATGAAACACAGCTTACCTGTTACAGGAGTAGTAGAAGCCGTTGTCGATGGAGTCCGCGCTGGTGTGCGTGATTTTGGTATTCAAGCCAACCTTATTGGCATTATGAGCCGCACCTTTGGTACCGATGCTTGCCAACAAGAGCTCGACGCCATCCTAAGCCAGAAAAATCACATTGTGGCAGTCGATCTCGCAGGTGATGAATTGGGGCAACCCGGTGACCGCTTTATCCAACATTTTAAACAAGTCAGAGATGCAGGATTGCATGTGACCGTTCACGCAGGTGAAGCGGCAGGGCCAGAAAGCATGTGGCAAGCGATCAGAGATCTGGGCGCAACAAGGATTGGTCATGGCGTCAAAGCCATCCATGATCCAAAACTGATGGACTATCTCGCCCAGCACCGGATCGGTATCGAATCATGCCTGACTTCCAACCTGCAGACGAGCACGGTGGATAGCCTAGCCACACATCCTCTTAAGCGTTTCTTAGAGCATGGTATTTTAGCCTGCATTAACACCGACGACCCTGCTGTAGAAGGTATTGAACTACCTTACGAATATGAAGTCGCAGCACCTCAAGCAGGCTTAAGCCAAGAGCAGATACGCCAAGCTCAGCTCAACGGTTTAGAGTTGGCCTTCCTCTCTGATTCAGAGAAAAAAGCGCTCCTCGCCAAAGCGGCACTACGCGGT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,326 total letters Query= IDP04388.104 Length=1407 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1808 0.0 1_09H_H9_IDP04388.104_T7S.seq 1654 0.0 2_12H_H9_IDP04388.104_T7term.seq 1563 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 58 TTCCCCTCTAGAAATAATTTTGTTTAACTTTAAG-AAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTG 146 1 9 ...T......N..-...........-......N.T......G.-..G..... 57 W S H P Q F E K G T E N L Y F Q S N A M I T S S L P L T D L Query_1 147 GTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGATAACTTCATCACTCCCTTTAACGGATTTGCA 236 0 1 ................................... 35 1 55 ................................... 89 H R H L D G N I R T Q T I L E L G Q K F G V K L P A N T Query_1 237 CCGTCATCTTGAT-GGAAATAT-CCGCACCCAAACCATTTTGGAATTAGGA--CAAAAATTTGG-CGTTAAGCTACCCGCAAAC-ACGTT 320 0 36 .............-........-............................--...........-...................-..... 119 1 90 .............-........-............................--...........-...................-..... 173 2 1044 .......N........T...........A.CA..........A..AC...........G.....G......N......C..... 961 L Q T L T P Y V Q I V E A E P S L V A F L S K L D W G V A Query_1 321 ACAAA-CTCTTACCCCTTATG-TACAAATTGTCGAAGCAGAACCCTCTCTGGTTGCCTTCTTATCCAAA-CTAGACTGGGGCGTAG-CGG 406 0 120 .....-...............-...............................................-................-... 205 1 174 .....-...............-...............................................-................-... 259 2 960 .....A....N......-...N..........A......AG.N...................N......AN....N..........G... 872 V L G D L D A C R R V A Y E N V E D A L N A R I D Y A E L Query_1 407 TACTAGGCGATCTTGATGCTTGCCG-TCGTGTTGCCTACGAGAACGTCGAAGATGCGCTTAACGCCC-GTATTGA-CTATGCCGAGCTAC 493 0 206 .........................-.........................................-.......-.............. 292 1 260 .........................-.........................................-.......-.............. 346 2 871 .........................G......N...........N......................N.......AN............. 782 R F S P Y Y M A M K H S L P V T G V V E A V V D G V R A G Query_1 494 GTTTTTCTCCTTATTACATGGCCATGAAA-CACAGCTTACCTGTTACAGG-AGTAGTAGAA-GCCGTTGTCGATGGAGTCCGCGCTGGTG 580 0 293 .............................-....................-..........-............................ 379 1 347 .............................-....................-..........-............................ 433 2 781 .............................A....................G..........A............................ 692 V R D F G I Q A N L I G I M S R T F G T D A C Q Q E L D A I Query_1 581 TGCGTGATTTTGGTATTCAAGCCAACCTTATTGGCATTATGAGCCGCACCTTTGGTACCGATGCTTGCCAACAAGAGCTCGACGCCATCC 670 0 380 .......................................................................................... 469 1 434 ......................................................................................N... 523 2 691 .......................................................................................... 602 L S Q K N H I V A V D L A G D E L G Q P G D R F I Q H F K Q Query_1 671 TAAGCCAGAAAAATCACATTGTGGCAGTCGATCTCGCAGGTGATGAATTGGGGCAACCCGGTGACCGCTTTATCCAACATTTTAAACAAG 760 0 470 .......................................................................................... 559 1 524 .......................................................................................... 613 2 601 .......................................................................................... 512 V R D A G L H V T V H A G E A A G P E S M W Q A I R D L G A Query_1 761 TCAGAGATGCAGGATTGCATGTGACCGTTCACGCAGGTGAAGCGGCAGGGCCAGAAAGCATGTGGCAAGCGATCAGAGATCTGGGCGCAA 850 0 560 .......................................................................................... 649 1 614 ...........N.............................................................................. 703 2 511 .......................................................................................... 422 T R I G H G V K A I H D P K L M D Y L A Q H R I G I E S C L Query_1 851 CAAGGATTGGTCATGGCGTCAAAGCCATCCATGATCCAAAACTGATGGACTATCTCGCCCAGCACCGGATCGGTATCGAATCATG-CCTG 939 0 650 .....................................................................................-.... 738 1 704 ...............................................................................T..N..C.... 793 2 421 .....................................................................................-.... 333 T S N L Q T S T V D S L A T H P L K R F L E H G I L A C I Query_1 940 ACTT-CCAACCTGCAGACGAGCACGGTGGATAGCCTAGCCACACATCCTCTTAAGCGTTTCTTAGAGCATGGTA-TTTTAGCCTGCATTA 1027 0 739 ....-.....................................................................-............... 826 1 794 .N..C..........N.........................................-......N.........T.....N......... 882 2 332 ....-.....................................................................-............... 245 N T D D P A V E G I E L P Y E Y E V A A P Q A G L S Q E Q I Query_1 1028 ACA-CCGACGACCCTGCTGTAGAAGGTATTGAACTACCTTACGAATATGAAGTCGCAGCACCTCAAGCAGGCTTAAGCCAAGAGCAGATA 1116 0 827 ...-...................................................................................... 915 1 883 ...N....................-...................................-...........C................. 970 2 244 ...-...................................................................................... 156 R Q A Q L N G L E L A F L S D S E K K A L L A K A A L R Query_1 1117 CGCCAAGCTCAGCTCAACGGTTTAGAGTTGGCCTTCCT-CTC-TGATTCAG-AGAAAAAAGCGCTCCT-CGCCAAAGCGGCAC-TACGCG 1201 0 916 ......................................-...-........-................-..............-...... 1000 1 971 .T..C......T.....................C....N...T......NNA................N.........C..N.T...... 1060 2 155 ......................................-...-........-................-..............-...... 71 G * H W K W I T D P N S S S V D K L A A A L E H H H H H H * Query_1 1202 GTTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccac-caccacTGA 1290 0 1001 .. 1002 2 70 ..C.T... 63 2 68 ..T..T.....AT.....T.A. 47 D P A A N K A R K E Query_1 1291 GATCCGGCTGCTAACAAAG-CCCGAAAGGAA 1320 2 46 ........N..........C.......A... 16 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4559698 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 3,326 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP09103.102 (IDP09103.1-562.F..pMCSG53)
MVKSKIYIDKIYWERVQLFVEGHSENLDLEDSNFVLRNLTETRTMKANDVKIDGNQFVCRFNVAILDNGYYLPEDKYLLVNEQELDYIAQLNPDVINDAYQNLKPEQEEEYNELETQNGKINFLLQTYLKEFRKGGISKKTVYTVTPEISSDVNEFVLDVVVTTPEVKSIYIVRKYKELRKYFRKQSFNTRQFIFKAIFNTTKFFHLKKGNTVLFTSDSRPTMSGNFEYIYNEMLRQNLDKKYDIHTVFKANITDRRGIIDKFRLPYLLGKADYIFVDDFHPLIYTVRFRRSQEVIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYVSSETDIPFYAEAFGIKEKNVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKVILFAPTFRGSGHGTAHYPFFKIDFERLARYCEKNNAVVLFKMHPFVKNRLNIADKHKQYFVDVSDFREVNDILFITDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEPYESFVPGKIVQSFDALMDALDNEDYEGEKVIPFLDKHFKYQDGRSSERLVRNLFGS
TTGGTTAAAAGTAAGATATATATAGATAAAATCTATTGGGAGCGTGTTCAATTATTCGTTGAAGGACATAGTGAAAACCTAGATTTAGAAGATAGTAATTTTGTATTAAGAAATTTAACTGAGACACGTACAATGAAAGCGAATGATGTCAAAATAGATGGGAATCAATTCGTTTGTCGTTTCAATGTAGCTATCTTAGATAATGGTTATTACTTACCTGAAGATAAGTACTTATTAGTGAATGAGCAAGAACTTGATTATATTGCACAGTTAAACCCAGATGTGATTAATGATGCATATCAAAATCTAAAGCCAGAACAAGAAGAAGAATACAACGAATTAGAAACACAAAATGGTAAAATCAATTTCTTATTGCAGACTTACCTAAAAGAATTTAGAAAAGGCGGCATTTCAAAGAAAACGGTTTATACTGTTACACCTGAAATTTCTAGCGATGTTAATGAATTTGTCCTTGATGTTGTTGTAACGACTCCGGAAGTTAAAAGTATTTATATCGTTCGTAAATATAAAGAATTACGTAAGTATTTCCGCAAACAATCATTTAATACAAGACAATTTATTTTTAAAGCGATATTTAATACGACGAAATTTTTCCACTTGAAAAAAGGGAATACGGTGTTGTTCACATCAGACTCTAGACCAACGATGTCTGGAAACTTTGAATACATCTATAACGAAATGTTACGTCAAAATTTAGATAAAAAGTATGATATTCACACTGTTTTTAAAGCGAATATTACAGATAGACGTGGCATCATCGACAAGTTTAGATTGCCATATTTACTTGGGAAGGCAGACTACATCTTTGTTGATGACTTTCACCCATTGATTTATACAGTGCGTTTTAGACGTTCTCAAGAAGTTATTCAAGTATGGCATGCCGTTGGTGCCTTTAAAACAGTTGGCTTTAGTCGTACTGGTAAAAAGGGTGGACCATTTATTGATTCATTAAATCATCGTAGCTATACAAAAGCTTATGTATCATCTGAAACCGATATTCCATTCTACGCTGAAGCATTTGGTATTAAAGAGAAAAATGTAGTGCCTACAGGTGTTCCACGTACTGATGTACTATTTGATGAAGCTTATGCGACACAGATCAAACAAGAGATGGAAGATGAATTACCAATTATTAAAGGTAAGAAAGTCATTCTTTTCGCACCAACATTTAGAGGTAGTGGTCATGGTACAGCACATTACCCATTTTTCAAAATTGATTTCGAACGTTTAGCAAGATATTGCGAAAAAAATAACGCGGTTGTATTATTTAAAATGCATCCATTTGTGAAAAATAGACTTAATATTGCAGACAAACATAAACAATATTTTGTTGACGTTTCTGACTTTAGAGAAGTTAATGATATACTGTTCATAACAGATTTATTAATTAGTGACTATTCATCTTTAATATATGAATATGCAGTATTTAAAAAGCCAATGATTTTCTATGCATTTGATTTAGAAGATTATATTACGACGCGTGATTTTTATGAGCCATATGAATCATTTGTTCCAGGTAAAATTGTGCAATCATTTGACGCATTAATGGACGCCTTGGACAATGAAGATTATGAAGGAGAAAAAGTCATTCCATTCTTAGATAAACATTTTAAATATCAAGATGGTCGATCAAGTGAGCGTTTAGTCAGAAATTTATTTGGTAGC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,165 total letters Query= IDP09103.102 Length=2091 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3041 0.0 2_10E_E7_IDP09103.102_T7term.seq 1864 0.0 1_07E_E7_IDP09103.102_T7S.seq 1824 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 56 AATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGT 145 1 5 ....T..........N-......................................................------------------- 74 W S H P Q F E K G T E N L Y F Q S N A L V K S K I Y I D K I Query_1 146 GGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCTTGGTTAAAAGTAAGATATATATAGATAAAATCT 235 0 1 .................................. 34 1 75 -----------------------------............................................................. 135 Y W E R V Q L F V E G H S E N L D L E D S N F V L R N L T E Query_1 236 ATTGGGAGCGTGTTCAATTATTCGTTGAAGGACATAGTGAAAACCTAGATTTAGAAGATAGTAATTTTGTATTAAGAAATTTAACTGAGA 325 0 35 .......................................................................................... 124 0 1477 ............ 1488 2 338 ............ 327 1 136 .......A........G......................................................................... 225 T R T M K A N D V K I D G N Q F V C R F N V A I L D N G Y Y Query_1 326 CACGTACAATGAAAGCGAATGATGTCAAAATAGATGGGAATCAATTCGTTTGTCGTTTCAATGTAGCTATCTTAGATAATGGTTATTACT 415 0 125 .......................................................................................... 214 1 226 .............G............................................................................ 315 L P E D K Y L L V N E Q E L D Y I A Q L N P D V I N D A Y Q Query_1 416 TACCTGAAGATAAGTACTTATTAGTGAATGAGCAAGAACTTGATTATATTGCACAGTTAAACCCAGATGTGATTAATGATGCATATCAAA 505 0 215 .......................................................................................... 304 0 235 ............ 224 1 316 .......................................................................................... 405 1 336 ............ 325 N L K P E Q E E E Y N E L E T Q N G K I N F L L Q T Y L K E Query_1 506 ATCTAAAGCCAGAACAAGAAGAAGAATACAACGAATTAGAAACACAAAATGGTAAAATCAATTTCTTATTGCAGACTTACCTAAAAGAAT 595 0 305 .......................................................................................... 394 2 573 ........... 583 1 406 .......................................................................................... 495 F R K G G I S K K T V Y T V T P E I S S D V N E F V L D V V Query_1 596 TTAGAAAAGGCGGCATTTCAAAGAAAACGGTTTATACTGTTACACCTGAAATTTCTAGCGATGTTAATGAATTTGTCCTTGATGTTGTTG 685 0 395 .......................................................................................... 484 1 496 ...................G...................................................................... 585 V T T P E V K S I Y I V R K Y K E L R K Y F R K Q S F N T R Query_1 686 TAACGACTCCGGAAGTTAAAAGTATTTATATCGTTCGTAAATATAAAGAATTACGTAAGTATTTCCGCAAACAATCATTTAATACAAGAC 775 0 485 .......................................................................................... 574 1 586 .......................................................................................... 675 Q F I F K A I F N T T K F F H L K K G N T V L F T S D S R Query_1 776 AATTTATTTTTAAAGCG-ATATTTAATACGACGAAATTTTTCCACTTGAAAAAAGGGAATACGGTGTTGTTCACATCAGA-CTCTAGACC 863 0 575 .................-..............................................................-......... 662 0 743 ...........A..... 759 2 1171 ...........N..AN.N..NNN. 1148 1 676 .................-..............................................................-......... 763 1 844 ...........A..... 860 P T M S G N F E Y I Y N E M L R Q N L D K K Y D I H T V F K Query_1 864 AACGATGTCTGGAAACTTTGAATACATCTATAACGAAATGTTACGTCAAAATTTAGATAAAAAGTATGATATTCAC-ACTGTTTTTAAAG 952 0 663 ............................................................................-............. 751 0 581 ......... 589 2 1147 NNN.NNN.......-......-.........NNN--..........TC......................NNNNN.C.N........... 1062 1 764 ............................................................................-............. 852 1 682 ......... 690 A N I T D R R G I I D K F R L P Y L L G K A D Y I F V D D F Query_1 953 CGAATATTACAGATAGACGTGGCATCATCGACAAGTTTAGATTGCCATATTT-ACTTGGGAAGGCAGACTACATCTTTGTTGATGACTTT 1041 0 752 ....................................................-..................................... 840 0 590 ..-..... 596 2 1061 NCG....-.............C...........G--................T......-..........N................... 976 1 853 ...................................-................-..................................... 940 1 691 ..-..... 697 H P L I Y T V R F R R S Q E V I Q V W H A V G A F K T V G F Query_1 1042 CACCCATTGATTTATACAGTGCGTTTTAGACGTTCTCAAGAAGTTATTCAAGTATGGCATGCCGTTGGTGCCTTTAAAACAGTTGGCTTT 1131 0 841 .......................................................................................... 930 2 975 ..........................-...................................................-........... 888 1 941 ...................-...-.........................-........................--.............. 1025 S R T G K K G G P F I D S L N H R S Y T K A Y V S S E T D Query_1 1132 AGTCGTACTGGTAAAAAGGGTGGACCATTTATTGATTCATTAAATCATCGTAGCTATACAAAAGCTTATGTATCATCTGAAA-CCGATAT 1220 0 931 ..................................................................................-....... 1019 2 887 ..................................................................................A....... 798 1 1026 .........NN.-............--A....N........N.............N......GNN.NN...........-..-N....N. 1110 I P F Y A E A F G I K E K N V V P T G V P R T D V L F D E Query_1 1221 TCCATTCTACGCTGAAGCATTTGGTATTAAAGAGAAAAA-TGTAGTGCCT-ACA-GGTGTTCCACGTACTGATGTA-CTATTTGATGAA- 1305 0 1020 .......................................-..........-...-.....................-............- 1104 2 797 .......................................A..........-...-.....................-............A 711 1 1111 ...NNN........-....-....NN........-...C-.NNNNG....N...G...NN..NN.NN.....N...C............- 1195 A Y A T Q I K Q E M E D E L P I I K G K K V I L F A P T F R Query_1 1306 GCTTATGCGACACAGATCAAACAAGAGATGGAAGATGAATTACCAATTATTAAAGGTAAGAAAGTCATTCTTTTCGCACCAACATTTAGA 1395 0 1105 .......................................................................................... 1194 2 710 .......................................................................................... 621 1 1196 ....... 1202 G S G H G T A H Y P F F K I D F E R L A R Y C E K N N A V V Query_1 1396 GGTAGTGGTCATGGTACAGCACATTACCCATTTTTCAAAATTGATTTCGAACGTTTAGCAAGATATTGCGaaaaaaaTAACGCGGTTGTA 1485 0 1195 .......................................................................................... 1284 2 620 ...............................................T.......................................... 531 L F K M H P F V K N R L N I A D K H K Q Y F V D V S D F R E Query_1 1486 TTATTTAAAATGCATCCATTTGTGAAAAATAGACTTAATATTGCAGACAAACATAAACAATATTTTGTTGACGTTTCTGACTTTAGAGAA 1575 0 1285 .......................................................................................... 1374 0 1637 ........... 1627 2 530 .......................................................................................... 441 2 178 ........... 188 V N D I L F I T D L L I S D Y S S L I Y E Y A V F K K P M I Query_1 1576 GTTAATGATATACTGTTCATAACAGATTTATTAATTAGTGACTATTCATCTTTAATATATGAATATGCAGTATTTAAAAAGCCAATGATT 1665 0 1375 .......................................................................................... 1464 2 440 .......................................................................................... 351 F Y A F D L E D Y I T T R D F Y E P Y E S F V P G K I V Q S Query_1 1666 TTCTATGCATTTGATTTAGAAGATTATATTACGACGCGTGATTTTTATGAGCCATATGAATCATTTGTTCCAGGTAAAATTGTGCAATCA 1755 0 1465 .......................................................................................... 1554 0 82 ............ 93 2 350 ..................................................A....................................... 261 1 183 ............ 194 F D A L M D A L D N E D Y E G E K V I P F L D K H F K Y Q D Query_1 1756 TTTGACGCATTAATGGACGCCTTGGACAATGAAGATTATGAAGGAGAAAAAGTCATTCCATTCTTAGATAAACATTTTAAATATCAAGAT 1845 0 1555 .......................................................................................... 1644 0 1296 ........... 1286 2 260 .......................................................................................... 171 2 519 ........... 529 G R S S E R L V R N L F G S * H W K W I T D P N S S S V D K Query_1 1846 GGTCGATCAAGTGAGCGTTTAGTCAGAAATTTATTTGGTAGCTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAG 1935 0 1645 .......................................... 1686 2 170 ..C....................................................................................... 81 L A A A L E H H H H H H * D P A A N K A R K E Query_1 1936 CTTGCGGCCGCACTCGAGcaccacc-accaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGC 2006 2 80 .........................A.............................................. 9 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 8532436 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 4,165 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP09104.102 (IDP09104.1-564.F..pMCSG53)
MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTETKTLEANEMKIDGNTFLCRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAYAALNEEQIEEYNELTTQNGKANYVLANEAKVFRKGGVSQHTVYTITPEIASDVNEFVFDIDITLPQEKSGVIATSAHWLHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFTSDSRPNLSGNFKYVYDELLRQKVDFDYDIKTVFKANITDRRKWRDKFRLPYLLGKADYIFVDDFHPLIYTVRFRPSQEIIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYVSSETDIPFYAEAFGIREENVVPTGVPRTDVLFDEAYATQIKQEMENELPLIKGKKVILFAPTFRGNGHGTAHYPFFKIDFERLARYCEKHNAVVLFKMHPFVKNRLNISREHRQYFIDVSDYREVNDILFFTDLLISDYSSLIYEYAVFKKPMIFYAFDLEYYITTRDFYEPYESFVPGKIVQSFDALMDALDNEDYEVEKVVPFLDKHFKYQDGRSSERLVKDLFRR
ATGACAAAAACGAAACAAGCAATACATATTGATAACATATACTGGGAACGTGTTCAGTTATATATTGAAGGACATAGTGAAGGTGTAGATTTAACATCAGGACAATTTGTTCTGAGGAATTTAACCGAAACAAAAACATTAGAAGCAAATGAAATGAAAATAGACGGTAATACATTTCTATGTAGATTCAATGTCGCAATATTAGACGATGGGTATTATTTACCAATGGATAAATATTTATTTGTTTATCATGACCAGTTAGAGTATATTGGACAACTTAATCCAAATATTATTGATCAAGCTTATGCGGCATTAAATGAAGAGCAAATTGAAGAATACAATGAGCTGACTACACAAAATGGAAAAGCAAACTATGTGTTAGCCAACGAAGCTAAAGTTTTCCGCAAAGGTGGCGTATCACAACATACGGTTTATACCATTACTCCAGAAATAGCAAGTGACGTTAACGAATTTGTATTTGATATTGACATCACCTTACCTCAAGAGAAATCAGGGGTCATTGCGACAAGCGCACACTGGCTTCATAAACAAGGCCATAAAGCTTCATTTGAAAGTAGAAGTTTCTTATTTAAAGCTATTTTTAATATTACAAAGTTACTACATATTAAAAGAAGCAAAACAATATTATTCACATCAGATTCACGTCCGAATTTATCAGGTAACTTTAAATATGTATATGATGAGTTACTACGCCAAAAAGTAGATTTTGATTATGATATTAAAACGGTATTTAAGGCGAATATTACGGATAGACGTAAATGGAGAGACAAGTTTAGATTGCCATATTTACTTGGTAAGGCTGATTATATTTTTGTCGATGATTTCCATCCATTAATTTATACGGTTCGCTTTAGACCATCACAAGAAATTATTCAAGTGTGGCATGCCGTTGGTGCTTTTAAAACAGTTGGCTTTAGTCGTACAGGTAAAAAAGGTGGTCCGTTTATTGATTCATTAAATCATCGTAGCTATACAAAAGCTTATGTATCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATGAAGCTTATGCAACACAAATTAAACAAGAGATGGAAAATGAATTACCACTTATAAAAGGTAAGAAAGTTATTCTATTCGCACCGACATTTAGAGGTAATGGTCACGGTACGGCACATTATCCATTTTTTAAAATTGATTTTGAACGTTTAGCAAGATATTGCGAGAAGCATAATGCAGTTGTGTTATTCAAAATGCATCCGTTCGTAAAAAATAGACTCAATATTTCACGTGAACATAGACAATACTTTATCGATGTGTCAGATTATCGTGAAGTTAACGATATTCTCTTTTTTACAGACTTGTTGATTAGTGATTATTCATCTTTAATATATGAATATGCAGTATTTAAAAAACCAATGATTTTCTATGCATTTGACTTAGAATATTATATTACGACGCGTGATTTCTATGAACCATATGAATCATTTGTTCCAGGTAAAATTGTACAATCCTTTGATGCATTAATGGATGCTTTGGACAATGAAGATTATGAGGTTGAAAAAGTTGTACCATTCTTAGATAAACATTTTAAATATCAAGATGGTCGCTCAAGTGAACGTTTAGTCAAAGATTTGTTTAGACGC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,313 total letters Query= IDP09104.102 Length=2097 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3052 0.0 1_07C_C7_IDP09104.102_T7S.seq 1653 0.0 2_10C_C7_IDP09104.102_T7term.seq 569 4e-165 Q F P S R N N F V * L * E G D I H M H H H H H H S S G V D Query_1 55 CAATTCCCCT-CTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCT 143 1 6 ..T.......T...................-..........................................----------------- 77 L W S H P Q F E K G T E N L Y F Q S N A M T K T K Q A I H I Query_1 144 GTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGACAAAAACGAAACAAGCAATACATATTGA 233 0 1 ................................ 32 1 78 -------------------------------........................................................... 136 D N I Y W E R V Q L Y I E G H S E G V D L T S G Q F V L R N Query_1 234 TAACATATACTGGGAACGTGTTCAGTTATATATTGAAGGACATAGTGAAGGTGTAGATTTAACATCAGGACAATTTGTTCTGAGGAATTT 323 0 33 .......................................................................................... 122 1 137 ......................................................C................................... 226 L T E T K T L E A N E M K I D G N T F L C R F N V A I L D D Query_1 324 AACCGAAACAAAAACATTAGAAGCAAATGAAATGAAAATAGACGGTAATACATTTCTATGTAGATTCAATGTCGCAATATTAGACGATGG 413 0 123 .......................................................................................... 212 1 227 .......................................................A.............C.................... 316 G Y Y L P M D K Y L F V Y H D Q L E Y I G Q L N P N I I D Q Query_1 414 GTATTATTTACCAATGGATAAATATTTATTTGTTTATCATGACCAGTTAGAGTATATTGGACAACTTAATCCAAATATTATTGATCAAGC 503 0 213 .......................................................................................... 302 0 241 ............ 230 1 317 .......................................................................................... 406 1 345 ............ 334 A Y A A L N E E Q I E E Y N E L T T Q N G K A N Y V L A N E Query_1 504 TTATGCGGCATTAAATGAAGAGCAAATTGAAGAATACAATGAGCTGACTACACAAAATGGAAAAGCAAACTATGTGTTAGCCAACGAAGC 593 0 303 .......................................................................................... 392 1 407 .................................................................TG......T.A.....GT.T..T.. 496 A K V F R K G G V S Q H T V Y T I T P E I A S D V N E F V F Query_1 594 TAAAGTTTTCCGCAAAGGTGGCGTATCACAACATACGGTTTATACCATTACTCCAGAAATAGCAAGTGACGTTAACGAATTTGTATTTGA 683 0 393 .......................................................................................... 482 1 497 ............T....................N.....C..............G................................... 586 D I D I T L P Q E K S G V I A T S A H W L H K Q G H K A S F Query_1 684 TATTGACATCACCTTACCTCAAGAGAAATCAGGGGTCATTGCGACAAGCGCACACTGGCTTCATAAACAAGGCCATAAAGCTTCATTTGA 773 0 483 .......................................................................................... 572 1 587 ......A.........................................T.......................T................. 676 E S R S F L F K A I F N I T K L L H I K R S K T I L F T S D Query_1 774 AAGTAGAAGTTTCTTATTTAAAGCTATTTTTAATATTACAAAGTTACTACATATTAAAAGAAGCAAAACAATATTATTCACATCAGATTC 863 0 573 .......................................................................................... 662 1 677 .......................................................................................... 766 S R P N L S G N F K Y V Y D E L L R Q K V D F D Y D I K T V Query_1 864 ACGTCCGAATTTATCAGGTAACTTTAAATATGTATATGATGAGTTACTACGCCAAAAAGTAGATTTTGATTATGATATTAAAACGGTATT 953 0 663 .......................................................................................... 752 1 767 G.................-..T..C..G.............................................................. 855 F K A N I T D R R K W R D K F R L P Y L L G K A D Y I F V D Query_1 954 TAAGGCGAATATTACGGATAGACGTAAATGGAGAGACAAGTTTAGATTGCCATATTTACTTGGTAA-GGCTGATTATATTTTTGTCGATG 1042 0 753 ..................................................................-....................... 841 1 856 .....A..................C..........................N..............G...C.......-......T.... 944 D F H P L I Y T V R F R P S Q E I I Q V W H A V G A F K T Query_1 1043 ATTTCCATCCATTAATTTATACGGTTCGCTTTAGACCATCACAAGAAATTATTC--AAG-TGTGGCATGCCGTTGGTG-CTTTTAAAACA 1128 0 842 ......................................................--...-..................-........... 927 0 1579 ........... 1569 1 945 ...............N......................................--...T...T......T.-.....-.C......... 1030 2 957 ...N.....N...A.....CN.NN-G.G...NG..T.C.N...G.C.NCNN.... 904 V G F S R T G K K G G P F I D S L N H R S Y T K A Y V Query_1 1129 GTTGGCTTTAGTCGTACAGGT---AAAAAAGGTGG-TCCGTTTATT-GATTCATTAAATC-ATCGTAGCTATACAAAAGCTTATGTAT-C 1211 0 928 .....................---...........-..........-.............-...........................-. 1010 1 1031 ..................A..---........-N.-AN..G....C-......-....C.-.......-..CCNG..NN.NA....T.-. 1110 2 903 NCN.T.G...N.......A.CNAA........N..G.NNN.NNN..C........C..N.C.N.....TN.CN.N..N..A.N...T.N. 814 S S E T D I P F Y A E A F G I R E E N V V P T G V P R T Query_1 1212 ATCAGAAACCGATATTCCATT--TTATG-CTGAA-GC-ATTTGGAATTAGAGAAGAA-AATGTTGTACCAACAGGTGTACCACGTACTG- 1294 0 1011 .....................--.....-.....-..-...................-...............................- 1093 1 1111 ..TNNG..NN..N........--.....C....G-..T......-NCNNNN....-N-N......N..N.N....-N...NNNNNNN..A 1193 2 813 T...NN.N...N.NA..NCN.CC.....-.....A..-.NC....NN...N......N...NA...N.......N..GTN...NNCG..- 727 D V L F D E A Y A T Q I K Q E M E N E L P L I K G K K V I L Query_1 1295 ATGTACTATTTGATGAAGCTTATGCAACACAAATTAAACAAGAGATGGAAAATGAATTACCACTTATAAAAGGTAAGAAAGTTATTCTAT 1384 0 1094 .......................................................................................... 1183 1 1194 ...N.NN......N...... 1213 2 726 ...N.N......N.....NNN....NN...C.C...TT...N.......NG....NNNN....NN.........GN......NN....N. 637 F A P T F R G N G H G T A H Y P F F K I D F E R L A R Y C E Query_1 1385 TCGCACCGACATTTAGAGGTAATGGTCACGGTACGGCACATTATCCATTTTTTAAAATTGATTTTGAACGTTTAGCAAGATATTGCGAGA 1474 0 1184 .......................................................................................... 1273 0 1662 ........... 1672 2 636 .N..N...N.N.NN......NN.....G.N.NGN...N.N.C......CN.......NN...NN......A.G.....N..GN....N.N 547 K H N A V V L F K M H P F V K N R L N I S R E H R Q Y F I D Query_1 1475 AGCATAATGCAGTTGTGTTATTCAAAATGCATCCGTTCGTAAAAAATAGACTCAATATTTCACGTGAACATAGACAATACTTTATCGATG 1564 0 1274 .......................................................................................... 1363 2 546 .....N......CN...N.....N.N.N.......N.N...N.......NNNT........N.N..G....C.N.N.C.N..N....... 457 V S D Y R E V N D I L F F T D L L I S D Y S S L I Y E Y A V Query_1 1565 TGTCAGATTATCGTGAAGTTAACGATATTCTCTTTTTTACAGACTTGTTGATTAGTGATTATTCATCTTTAATATATGAATATGCAGTAT 1654 0 1364 .......................................................................................... 1453 2 456 N.N.N.N.N......N..NNNT...NN.......NG..N...C.NN......N....N.N.........NT.....N...N......N.N 367 F K K P M I F Y A F D L E Y Y I T T R D F Y E P Y E S F V P Query_1 1655 TTAAAAAACCAATGATTTTCTATGCATTTGACTTAGAATATTATATTACGACGCGTGATTTCTATGAACCATATGAATCATTTGTTCCAG 1744 0 1454 .......................................................................................... 1543 2 366 ..N....G..GNN...G.....N...NCN.N.N.....G.NN.C.NN........N...NNN.N...N...NCN.......CN...N... 277 G K I V Q S F D A L M D A L D N E D Y E V E K V V P F L D K Query_1 1745 GTAAAATTGTACAATCCTTTGATGCATTAATGGATGCTTTGGACAATGAAGATTATGAGGTTGAAAAAGTTGTACCATTCTTAGATAAAC 1834 0 1544 .......................................................................................... 1633 0 857 ........... 847 2 276 N.NG...N.....GN.N.CN......NC......N...NN..N.N.C......N.......G...N...NN..G...NN.......G.T. 187 H F K Y Q D G R S S E R L V K D L F R R * H W K W I T D P N Query_1 1835 ATTTTAAATATCAAGATGGTCGCTCAAGTGAACGTTTAGTCAAAGATTTGTTTAGACGCTAACATTGGAAGTGGATAACGGATCCGAATT 1924 0 1634 ........................................................... 1692 0 1248 ........... 1258 2 186 ..CN..........N.....N.NN...NN......NN.....NN...NN...GT.N...N.N...C...N.N............N.N.NN 97 S S S V D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 1925 CGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAG 2011 2 96 ....N....N..N.N.C.N......N..C.C....................................-N..C............... 11 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 8864960 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 4,313 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP09105.102 (IDP09105.1-349.F..pMCSG53)
MITPQVIDKPHIVFMMTFPEDIKPIIKALNNSSYQKTVLTTPKQAPYLSELSDDVDVIEMTNRTLVKQIKALKSAQMIIIDNYYLLLGGYNKTSNQHIVQTWHASGALKNFGLTDHQVDVSDKAMVQQYRKVYQATDFYLVGCEQMSQCFKQSLGATEEQMLYFGLPRINKYYTADRATVKAELKDKYGITNKLVLYVPTYREDKADNRAIDKAYFEKCLPGYTLINKLHPSIEDSDIDDVSSIDTSTLMLMSDIIISDYSSLPIEASLLDIPTIFYVYDEGTYDQVRGLNQFYKAIPDSYKVYTEEDLIMTIQEKEHLLSPLFKDWHKYNTDKSLHQLTEYIDKMVTK
ATGATTACGCCTCAAGTGATTGATAAACCGCATATCGTATTTATGATGACTTTTCCAGAAGATATTAAGCCTATCATCAAAGCATTAAATAATTCGTCGTATCAGAAAACTGTTTTAACAACACCAAAACAAGCGCCTTATTTATCTGAACTTAGCGACGATGTTGATGTGATAGAAATGACTAATCGAACATTGGTAAAACAAATTAAGGCTTTGAAAAGCGCGCAGATGATTATTATCGATAATTATTACCTATTACTAGGTGGATATAATAAGACTTCTAATCAACACATTGTTCAAACGTGGCATGCAAGTGGTGCATTAAAAAACTTTGGCTTAACAGATCATCAAGTCGATGTGTCTGACAAGGCAATGGTTCAGCAGTACCGTAAAGTTTATCAAGCGACGGATTTTTACTTAGTGGGTTGTGAACAAATGTCACAATGTTTTAAACAGTCTTTAGGTGCAACAGAAGAGCAAATGCTGTATTTTGGGCTTCCGAGAATTAATAAATATTACACAGCTGATAGAGCAACGGTTAAGGCAGAGTTAAAGGATAAATATGGAATTACAAATAAGTTGGTATTATATGTACCAACATATAGAGAAGATAAAGCAGATAATAGGGCTATTGATAAAGCTTATTTTGAAAAATGTTTACCAGGATATACACTGATTAATAAATTACATCCATCAATTGAAGATTCAGACATTGATGACGTATCTTCAATCGACACGTCTACATTAATGCTAATGTCAGATATAATTATTAGCGACTATAGTTCGCTGCCAATAGAAGCTAGCTTGTTAGATATTCCAACTATATTTTATGTGTATGATGAAGGAACATATGATCAGGTGAGAGGCCTGAATCAATTTTACAAAGCAATACCGGATAGCTACAAAGTGTATACTGAAGAAGATTTAATAATGACGATACAAGAAAAAGAACATCTATTAAGTCCGTTATTTAAAGATTGGCATAAGTATAATACTGATAAAAGTTTACATCAGCTCACAGAATATATAGATAAGATGGTGACAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,447 total letters Query= IDP09105.102 Length=1452 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1889 0.0 2_10D_D7_IDP09105.102_T7term.seq 1840 0.0 1_07D_D7_IDP09105.102_T7S.seq 1723 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 60 CCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTC 149 1 13 ...........-.......................................................----------------------- 78 S H P Q F E K G T E N L Y F Q S N A M I T P Q V I D K P H I Query_1 150 TCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGATTACGCCTCAAGTGATTGATAAACCGCATATCGT 239 0 1 ...................................... 38 2 1141 ......N.. 1133 1 79 -------------------------................................................................. 143 V F M M T F P E D I K P I I K A L N N S S Y Q K T V L T T P Query_1 240 ATTTATGATGACTTTTCCAGAAG-ATATTAAGCCTATCATCAAAGCATTAAATAATTCGTCGTATCAGAAAACTGTTTTAACAACACCAA 328 0 39 .......................-.................................................................. 127 2 1132 .A..N..N-........-..N..N......-...NT....N..-....---.NN......TCGN......N-.............G...G 1051 1 144 .......................-.................................................................. 232 K Q A P Y L S E L S D D V D V I E M T N R T L V K Q I K A L Query_1 329 AACAAGCGCCTTATTTATCTGAACTTAGCGACGATGTTGATGTGATAGAAATGACTAATCGAACATTGGTAAAACAAATTAAGGCTTTGA 418 0 128 .......................................................................................... 217 2 1050 .C.......................G..N.....N.......N.......-..................N.....N.............. 962 1 233 .......................................................................................... 322 K S A Q M I I I D N Y Y L L L G G Y N K T S N Q H I V Q T W Query_1 419 AAAGCGCGCAGATGATTATTATCGATAATTATTACCTATTACTAGGTGGATATAATAAGACTTCTAATCAACACATTGTTCAAACG-TGG 507 0 218 ......................................................................................-... 306 0 249 ..A............A.. 232 2 961 .................................N....................................................G... 872 2 930 ..A............A.. 947 1 323 ......................................................................................-... 411 1 354 ..A............A.. 337 H A S G A L K N F G L T D H Q V D V S D K A M V Q Q Y R K V Query_1 508 CATGCAAGTGGTGCATTAAAAAACTTTGGCTTAACAGATCATCAAGTCGATGTGTCTGACAAGGCAATGGTTCAGCAGTACCGTAAAGTT 597 0 307 .......................................................................................... 396 2 871 .......................................................................................... 782 1 412 .......................................................................................... 501 Y Q A T D F Y L V G C E Q M S Q C F K Q S L G A T E E Q M Query_1 598 TATCAAGCGACGGATTTTTACTTAGTGGGTTGTGAACAAATGTCACAATGTTTTAAA-CAGTCTTTAGGTGCAACAGAAGAGCAAATGCT 686 0 397 .........................................................-................................ 485 2 781 .........................................................A................................ 692 1 502 .........................................................-................................ 590 L Y F G L P R I N K Y Y T A D R A T V K A E L K D K Y G I T Query_1 687 GTATTTTGGG-CTTCCGAGAATT-AATAAATATTACACAGCTGATAGAGCAACGGTTAAGGCAGAGTTAAAGGATAAATATGGAATTACA 774 0 486 ..........-............-.................................................................. 573 2 691 ..........G............-.................................................................. 603 1 591 ..........-............N.................................................................. 679 N K L V L Y V P T Y R E D K A D N R A I D K A Y F E K C L Query_1 775 AATAAGTTGGTATTATATGTACCAACATATAGAGAAGATAAAGCAGATAATAGGGCTATTGATAAAGC-TTATTTTGAAAAATGTTTACC 863 0 574 ....................................................................-..................... 662 2 602 ....................................................................-..................... 514 1 680 ....................................................................T..................... 769 P G Y T L I N K L H P S I E D S D I D D V S S I D T S T L M Query_1 864 AGGATATACACTGATTAATAAATTACATCCATCAATTGAAGATTCAGACATTGATGACGTATCTTCAATCGACACGTCTACATTAATGCT 953 0 663 .......................................................................................... 752 2 513 .......................................................................................... 424 1 770 .......................................................................................... 859 L M S D I I I S D Y S S L P I E A S L L D I P T I F Y V Y D Query_1 954 AATGTCAGATATAATTATTAGCGACTATAGTTCGCTGCCAATAGAAGCTAGCTTGTTAGATATTCCAACTATATTTTATGTGTATGATGA 1043 0 753 .......................................................................................... 842 2 423 .......................................................................................... 334 1 860 .....................................................G.......N....................N....... 949 E G T Y D Q V R G L N Q F Y K A I P D S Y K V Y T E E D L I Query_1 1044 AGGAACATATGATCAGGTGAGAGGCCTGAATCAATTTTACAAAGCAATACCGGATAGCTACAAAGTGTATACTGAAGAAGATTTAATAAT 1133 0 843 .......................................................................................... 932 2 333 .......................................................................................... 244 1 950 ........................N....N..-............-........NTN....-............-......-.....-.. 1033 M T I Q E K E H L L S P L F K D W H K Y N T D K S L H Q L T Query_1 1134 GACGATACAAGAAAAAGAACATCTATTAAGTCCGTTATTTAAAGATTGGCATAAGTATAATACTGATAAAAGTTTACATCAGCTCACAGA 1223 0 933 .......................................................................................... 1022 2 243 .......................................................................................... 154 1 1034 .....N...--G...............T.........-.......-.N.....--NNNN............... 1101 E Y I D K M V T K * H W K W I T D P N S S S V D K L A A A L Query_1 1224 ATATATAGATAAGATGGTGACAAAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGA 1313 0 1023 ......................... 1047 2 153 .......................................................................................... 64 E H H H H H H * D P A A N K A R K E Query_1 1314 GcaccaccaccaccaccacTGAGATCCGGCTGCT-AACAAAGCCCGAAA-GGAA 1365 2 63 ..................................A......N...N...N.... 10 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4880964 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 3,447 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP10010.102 (IDP10010.1-65.F..pMCSG53)
MFDTLSKAGKYLGQAAKMMIGVPDYDNYVEHMRITHPDQTPMTYEEFFRERQDARYGGKGGARCC
ATGTTCGATACGCTTTCGAAAGCCGGAAAATATCTGGGACAGGCCGCAAAAATGATGATTGGCGTGCCAGACTACGACAACTATGTGGAACATATGCGCATAACGCATCCGGATCAGACGCCGATGACCTATGAAGAATTTTTCCGCGAGCGGCAGGATGCGCGCTATGGTGGAAAAGGCGGCGCGCGTTGCTGC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,415 total letters Query= IDP10010.102 Length=600 Score E Sequences producing significant alignments: (Bits) Value 1_04D_D4_IDP10010.102_T7S.seq 801 0.0 2_10D_D4_IDP10010.102_T7term.seq 731 0.0 0_wt 352 8e-101 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAA 90 1 19 ................................. 51 2 485 .......................................................................................... 396 2 418 ...G...........T.T.C.C.C...........A... 456 E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E N Query_1 91 GAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAAC 180 1 52 ....................................------------------------------------------------...... 93 2 395 ....................................------------------------------------------------...... 354 L Y F Q S N A M F D T L S K A G K Y L G Q A A K M M I G V P Query_1 181 CTGTACTTCCAATCCAATGCCATGTTCGATACGCTTTCGAAAGCCGGAAAATATCTGGGACAGGCCGCAAAAATGATGATTGGCGTGCCA 270 1 94 .......................................................................................... 183 1 137 ............ 126 2 353 .......................................................................................... 264 2 310 ............ 321 0 1 ..................................................................... 69 0 23 ............ 12 D Y D N Y V E H M R I T H P D Q T P M T Y E E F F R E R Q D Query_1 271 GACTACGACAACTATGTGGAACATATGCGCATAACGCATCCGGATCAGACGCCGATGACCTATGAAGAATTTTTCCGCGAGCGGCAGGAT 360 1 184 .......................................................................................... 273 2 263 .......................................................................................... 174 0 70 .......................................................................................... 159 A R Y G G K G G A R C C * H W K W I T D P N S S S V D K L A Query_1 361 GCGCGCTATGGTGGAAAAGGCGGCGCGCGTTGCTGCTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCG 450 1 274 .......................................................................................... 363 2 173 .......................................................................................... 84 0 160 .................................... 195 A A L E H H H H H H * D P A A N K A R K E A E L A A A T A Query_1 451 GCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAG-CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGA 539 1 364 ....................................................-..................................... 452 2 83 ............................................N.......C....... 24 E Q * L A * P L G A S K R V L R G F L L K Query_1 540 GCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAA 600 1 453 ............................................................. 513 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1390578 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 2,415 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP10086.102 (IDP10086.1-268.F..pMCSG53)
MRLVLEEPFKRLWNGRDPFEAVEALQGKVYRELEGRRTLRTEVDGRGYFVKIHRGIGWGEIAKNLLTAKLPVLGARQEWQAIRRLHEAGVATMTAVAYGERGSDPARQHSFIVTEELAPTVDLEVFSQDWRERPPPPRLKRALVEAVARMVGDMHRAGVNHRDCYICHFLLHTDKPVSADDFRLSVIDLHRAQTRDATPKRWRNKDLAALYFSALDIGLTRRDKLRFLRTYFRRPLREILRDEAGLLAWMERKAEKLYERKQRYGDLL
ATGAGGCTGGTGCTGGAAGAGCCGTTCAAGCGCCTGTGGAACGGGCGCGACCCGTTCGAGGCGGTGGAGGCGCTGCAAGGCAAGGTCTACCGCGAACTGGAAGGGCGCCGCACCCTGCGCACCGAGGTCGACGGGCGTGGCTACTTCGTCAAGATCCACCGTGGCATCGGCTGGGGCGAGATCGCCAAGAACCTGCTCACCGCCAAGCTCCCGGTGCTCGGCGCGCGCCAGGAGTGGCAGGCCATCCGGCGCCTGCACGAGGCCGGCGTAGCGACCATGACCGCGGTCGCCTACGGCGAGCGCGGCAGCGATCCGGCGCGGCAGCATTCCTTCATCGTCACCGAGGAACTGGCGCCGACCGTGGACCTCGAGGTGTTCTCCCAGGACTGGCGCGAACGTCCTCCACCGCCGCGGCTCAAGCGCGCGCTGGTCGAGGCGGTGGCGCGGATGGTCGGCGACATGCACCGTGCCGGAGTCAACCATCGCGACTGCTACATCTGTCATTTCCTGTTGCACACCGACAAGCCGGTGAGCGCGGACGATTTCCGCCTCTCGGTGATCGATCTGCACCGTGCCCAGACCCGCGACGCCACGCCGAAACGCTGGCGTAACAAGGATCTGGCGGCATTGTATTTCTCTGCGCTGGACATCGGACTGACGCGTCGCGACAAGCTACGCTTCCTGCGCACCTATTTCCGCCGGCCGTTGCGCGAGATACTGCGCGACGAGGCCGGCCTGCTGGCCTGGATGGAACGCAAGGCGGAAAAACTCTACGAACGCAAGCAGCGTTACGGAGACCTGCTC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,312 total letters Query= IDP10086.102 Length=1209 Score E Sequences producing significant alignments: (Bits) Value 1_03C_C3_IDP10086.102_T7S.seq 1775 0.0 2_09C_C3_IDP10086.102_T7term.seq 1761 0.0 0_wt 1451 0.0 S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 7 TCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGA-TAACAATTCCCCTCTAG--AAATAATTTTG-TTTAACTTTAAGA 92 1 20 .........--..-........-............. 51 2 1088 ..........................NTA................N...........-.....GA..N........G............. 1000 E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E N Query_1 93 AGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCT 182 1 52 ..................................------------------------------------------------........ 93 2 999 .A................................------------------------------------------------........ 958 L Y F Q S N A M R L V L E E P F K R L W N G R D P F E A V E Query_1 183 GTACTT-CCAATCCAATGCCATGAGGCTGGTGCTGG-AAGAGCCGTTCAAGCGCCTGTGGAACGGGCGCGACCCGTTCGAGGCGGTGGAG 270 1 94 ......-.............................-..................................................... 181 1 532 .....CG.T.G............ 554 1 525 ..C.......... 513 2 957 .GN...T.............................N..................................................... 868 2 517 .....CG.T.G............ 495 2 524 ..C.......... 536 0 1 ................-..................................................... 69 0 420 .....CG.T.G............ 442 0 413 ..C.......... 401 A L Q G K V Y R E L E G R R T L R T E V D G R G Y F V K I H Query_1 271 GCGCTGCAAGGCAAGGTCTACCGCGAACTGGAAGGGCGCCGCACCCTGCGCACCGAGGTCGACGGGCGTGGCTACTTCGTCAAGATCCAC 360 1 182 .......................................................................................... 271 1 512 . 512 2 867 .......................................................................................... 778 2 537 . 537 0 70 .......................................................................................... 159 0 400 . 400 R G I G W G E I A K N L L T A K L P V L G A R Q E W Q A I R Query_1 361 CGTGGCATCGGCTGGGGCGAGATCGCCAAGAACCTGCTCACCGCCAAGCTCCCGGTGCTCGGCGCGCGCCAGGAGTGGCAGGCCATCCGG 450 1 272 .......................................................................................... 361 2 777 .......................................................................................... 688 0 160 .......................................................................................... 249 R L H E A G V A T M T A V A Y G E R G S D P A R Q H S F I V Query_1 451 CGCCTGCACGAGGCCGGCGTAGCGACCATGACCGCGGTCGCCTACGGCGAGCGCGGCAGCGATCCGGCGCGGCAGCATTCCTTCATCGTC 540 1 362 .......................................................................................... 451 1 837 ........... 847 2 687 .......................................................................................... 598 2 212 ........... 202 0 250 .......................................................................................... 339 0 725 ........... 735 T E E L A P T V D L E V F S Q D W R E R P P P P R L K R A L Query_1 541 ACCGAGGAACTGGCGCCGACCGTGGACCTCGAGGTGTTCTCCCAGGACTGGCGCGAACGTCCTCCACCGCCGCGGCTCAAGCGCGCGCTG 630 1 452 .......................................................................................... 541 1 157 .....AC.C. 166 1 182 ...........T.. 169 2 597 .......................................................................................... 508 2 892 .....AC.C. 883 2 867 ...........T.. 880 0 340 .......................................................................................... 429 0 45 .....AC.C. 54 0 70 ...........T.. 57 V E A V A R M V G D M H R A G V N H R D C Y I C H F L L H T Query_1 631 GTCGAGGCGGTGGCGCGGATGGTCGGCGACATGCACCGTGCCGGAGTCAACCATCGCGACTGCTACATCTGTCATTTCCTGTTGCACACC 720 1 542 .......................................................................................... 631 1 167 T............ 179 1 678 ........... 688 2 507 .......................................................................................... 418 2 882 T............ 870 2 371 ........... 361 0 430 .......................................................................................... 519 0 55 T............ 67 0 566 ........... 576 D K P V S A D D F R L S V I D L H R A Q T R D A T P K R W R Query_1 721 GACAAGCCGGTGAGCGCGGACGATTTCCGCCTCTCGGTGATCGATCTGCACCGTGCCCAGACCCGCGACGCCACGCCGAAACGCTGGCGT 810 1 632 .......................................................................................... 721 1 573 ........... 583 2 417 .......................................................................................... 328 2 476 ........... 466 0 520 .......................................................................................... 609 0 461 ........... 471 N K D L A A L Y F S A L D I G L T R R D K L R F L R T Y F R Query_1 811 AACAAGGATCTGGCGGCATTGTATTTCTCTGCGCTGGACATCGGACTGACGCGTCGCGACAAGCTACGCTTCCTGCGCACCTATTTCCGC 900 1 722 .......................................................................................... 811 2 327 .......................................................................................... 238 0 610 .......................................................................................... 699 R P L R E I L R D E A G L L A W M E R K A E K L Y E R K Q R Query_1 901 CGGCCGTTGCGCGAGATACTGCGCGACGAGGCCGGCCTGCTGGCCTGGATGGAACGCAAGGCGGAAAAACTCTACGAACGCAAGCAGCGT 990 1 812 ..................................................................................N....... 901 1 369 ........... 379 2 237 .......................................................................................... 148 2 680 ........... 670 0 700 .......................................................................................... 789 0 257 ........... 267 Y G D L L * H W K W I T D P N S S S V D K L A A A L E H H Query_1 991 TACGGAGACCTGCTCTAACATTGGAAGTGGATAACGGATCCGAA-TTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccacca 1079 1 902 ............................................N............................................. 991 2 147 ............................................-............................................. 59 0 790 ............... 804 H H H H * D P A A N K A R K E A E L A A A T A E Q * L A * Query_1 1080 ccaccaccacTGAGAT-CCGGC-TGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGC-ATAACC 1166 1 992 ................C.....T....-..N.....N.-...........N..N.................N...........A.N.C.. 1079 2 58 ................-.....-...................A.. 16 P L G A S K R V L R G F L Query_1 1167 CCTTGGGGCCTCTAAACGGGTCTT-GAGGGGTTTTTTGCT 1205 1 1080 ..NG.........-.....TCN..G............... 1118 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3893952 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 3,312 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP10113.102 (IDP10113.1-352.F..pMCSG53)
MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQDTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAILVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQTTMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEVVLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHIAAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAADVIAAVDKLLPSSTTGTSL
GTGGATAAGCCATTTCGAAGAATTTTGCTCATTAAGATGCGTTTTCATGGGGATATGTTATTAACTACTCCCGTCATTAGTTCTCTGAAAAAAAATTACCCTGACGCAAAAATCGATGTGCTGCTTTATCAGGACACCATCCCGATCCTGTCTGAAAATCCAGAGATTAACGCGCTCTACGGCATAAAAAATAAAAAAGCAAAAGCCTCAGAAAAAATTGCCAACTTTTTTCATCTCATCAAGGTATTACGTGCCAATAAGTATGACCTTATCGTCAATCTTACCGATCAATGGATGGTTGCTATACTGGTTCGCTTATTAAATGCTCGTGTGAAAATTTCCCAGGATTATCATCATCGGCAGTCTGCTTTTTGGCGTAAAAGTTTCACCCATTTGGTGCCGTTGCAGGGTGGAAATGTGGTGGAAAGTAACTTATCCGTGCTGACACCATTGGGACTTGATTCGTTGGTGAAGCAGACAACCATGAGTTACCCGCCTGCAAGCTGGAAACGTATGCGTCGCGAACTTGATCACGCTGGTGTTGGACAAAATTATGTGGTTATCCAACCTACGGCGCGGCAAATCTTCAAATGCTGGGACAACGCCAAGTTTTCCGCTGTGATTGATGCCTTACATGCTCGTGGTTATGAAGTCGTTCTGACGTCCGGCCCAGATAAAGACGATCTGGCCTGCGTCAATGAAATTGCGCAGGGATGCCAGACGCCACCAGTAACGGCGCTGGCTGGAAAGGTGACCTTCCCGGAACTTGGTGCGTTAATCGATCATGCGCAGCTGTTTATTGGCGTTGATTCCGCACCGGCGCATATTGCCGCTGCAGTTAATACGCCGCTGATATCGCTGTTTGGCGCAACAGATCATATTTTCTGGCGTCCCTGGTCAAATAACATGATTCAATTCTGGGCGGGAGATTACCGGGAAATGCCAACGCGCGATCAGCGTGACCGAAATGAGATGTATCTTTCGGTTATTCCGGCGGCAGATGTCATTGCTGCTGTCGATAAATTACTGCCCTCCTCCACGACAGGTACGTCGTTA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,582 total letters Query= IDP10113.102 Length=1461 Score E Sequences producing significant alignments: (Bits) Value 2_12F_F6_IDP10113.102_T7term.seq 1923 0.0 0_wt 1905 0.0 1_06F_F6_IDP10113.102_T7S.seq 1801 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 58 TTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGG 147 1 18 .............-.................................................N.....--------------------- 85 S H P Q F E K G T E N L Y F Q S N A V D K P F R R I L L I Query_1 148 TCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGTGGA-TAAGCCATTTCGAAGAATTTTGCTCATTAA 236 2 1207 .............A...NNNNN.....G.N....GNTC...... 1164 0 1 .....-.............................. 35 1 86 ---------------------------................................-.............................. 147 K M R F H G D M L L T T P V I S S L K K N Y P D A K I D V Query_1 237 GATGCGTTTTCATGGGGA-TATGTTATTAACTACTCCCGTCATTAGTTCTCTG--aaaaaaaaTTACCCTGACGCAAAAATCGATGTGCT 323 2 1163 ....N.............A.....-....G-...N............-.N...GA........A..N............-.......... 1078 0 36 ..................-..................................--................................... 122 1 148 ..................-..................................--................................... 234 L L Y Q D T I P I L S E N P E I N A L Y G I K N K K A K A Query_1 324 -GCTTTATCAGGACACCA-TCCCGATCCTGTCTGAAAATCC-AGAGATTAACGCGCTCTACGG-CATaaaaaataaaaaagcaaaagcct 409 2 1077 T.................A......................C.....................G........TA...........-.... 989 0 123 -.................-......................-.....................-.......................... 208 1 235 -.................-......................-.....................-.......................... 320 S E K I A N F F H L I K V L R A N K Y D L I V N L T D Q W Query_1 410 cag-aaaaaaTTGCCAACTTTTTTCATCTCATCAAGGTATTACGTGCCAATAAGTA-TGACCTTATCGTCAATCTTACCGATCAATGGAT 497 2 988 ...G....................................................A.....................N........... 899 0 209 ...-....................................................-................................. 296 1 321 ...-....................................................-................................. 408 M V A I L V R L L N A R V K I S Q D Y H H R Q S A F W R K S Query_1 498 GGTTGCTATACTGG-TTCGCTTATTAAATGCTCGTGTGAAAATTTCCCAGGATTATCATCATCGG-CAGTCTGCTTTTTGGCGTAAAAGT 585 2 898 ..............N..................................................G........................ 809 0 297 ..............-..................................................-........................ 384 1 409 ..............-..................................................-........................ 496 F T H L V P L Q G G N V V E S N L S V L T P L G L D S L V K Query_1 586 TTCACCCATTTGGTGCCGTTGCAGGGTGGAAATGTGGTGGAAAGTAACTTATCCGTGCTGACACCATTGGGACTTGATTCGTTGGTGAAG 675 2 808 .......................................................................................... 719 0 385 .......................................................................................... 474 1 497 .......................................................................................... 586 Q T T M S Y P P A S W K R M R R E L D H A G V G Q N Y V V I Query_1 676 CAGACAACCATGAGTTACCCGCCTGCAAGCTGGAAACGTATGCGTCGCGAACTTGATCACGCTGGTGTTGGACAAAATTATGTGGTTATC 765 2 718 .......................................................................................... 629 0 475 .......................................................................................... 564 1 587 .......................................................................................... 676 Q P T A R Q I F K C W D N A K F S A V I D A L H A R G Y E V Query_1 766 CAACCTACGGCGCGGCAAATCTTCAAATGCTGGGACAACGCCAAGTTTTCCGCTGTGATTGATGCCTTACATGCTCGTGGTTATGAAGTC 855 2 628 .......................................................................................... 539 0 565 .......................................................................................... 654 1 677 .......................................................................................... 766 V L T S G P D K D D L A C V N E I A Q G C Q T P P V T A L Query_1 856 G-TTCTGACGTCCGGCCCAGATAAAGACGATCTGGCCTGCGTCAATGAAATTGCGCAGGGATGCCAGACGCCACCAGTAACGGCGCTGGC 944 2 538 .-........................................................................................ 450 0 655 .-........................................................................................ 743 1 767 .T........................................................................................ 856 A G K V T F P E L G A L I D H A Q L F I G V D S A P A H I A Query_1 945 TGGAAAGGTGACCTTCCCGGAACTTGGTGCGTTAATCGA-TCATGCGCAGCTGTTTATTGGCGTTGATTCCGCACCGGCGCATATTGCCG 1033 2 449 .......................................-.................................................. 361 0 744 .......................................-.................................................. 832 1 857 .......................................N.................................................. 946 A A V N T P L I S L F G A T D H I F W R P W S N N M I Q F Query_1 1034 CTGCAGTTAATACGCCGCTG-ATATCGCTGTTTGGCGCAACAGATCATA-TTTTCTGGCGTCCCTGGTCAAATAACATGATTCAATTCTG 1121 2 360 ....................-............................-........................................ 273 0 833 ....................-............................-........................................ 920 1 947 ....................A.................-..........T...........-.......-....N............... 1033 W A G D Y R E M P T R D Q R D R N E M Y L S V I P A A D V Query_1 1122 GGCGGGAGATTACCGGGAAATGCCAA-CGCGCGATCAGCGTGACCGAAATGAGATGTA-TCTTTCGGTTATTCCGG-CGGCAG-ATGTCA 1207 2 272 ..........................-...............................-.................-......-...... 187 0 921 ..........................-...............................-.................-......-...... 1006 1 1034 ...-.............G........N..........................N....T.....TC.GNN......C......A.....N 1122 I A A V D K L L P S S T T G T S L * H W K W I T D P Query_1 1208 TTGCTGCTG-TCGATAA--ATTACTGCCCTCC-TCCAC-GA-CAGGT-ACG-TCGTTA-TAACATTGGAAGTGG--ATAACGG--ATCCG 1284 2 186 .........-.......--.............-.....-..-.....-...-......-...............--.......--..... 110 0 1007 .........-.......--.............-.....-..-.....-...-...... 1056 1 1123 .........T.......AT..NC..NN.....T...N.N..N.N...N...T......N..N.....NNN.N..NN....N..NA..... 1212 N S S S V D K L A A A L E H H H H H H * D P A A N K A R Query_1 1285 AA-TTCGAGCTCC-GTCGACAA-GCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAA--GCCCGAA 1369 2 109 ..-..........-........-..........................................................AN....... 23 1 1213 N.T...N.NN...NN.......G.N.....NNNC..... 1251 Query_1 1370 A 1370 2 22 . 22 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5106630 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 3,582 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP10173.102 (IDP10173.29-350.P(350)..pMCSG53)
DSRLGTFSQIRTYMDTAVSPFYFISNGPRELLDSVSQTLASRDQLELENRALRQELLLKNSDLLMLGQYKQENARLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQVVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGDVLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNGANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPPAPVPDPATGITQPSAGQTAPVSTQPSPSGATTPPARAPGG
GACAGCCGCCTGGGGACGTTCAGTCAAATCCGAACGTACATGGACACTGCCGTCAGTCCTTTCTATTTTATTTCAAACGGCCCTCGTGAACTGCTCGACAGCGTGTCGCAAACGCTGGCTTCTCGCGACCAGCTTGAGCTGGAAAATCGGGCGCTGCGCCAGGAATTACTGCTAAAAAACAGCGACCTGCTGATGCTGGGGCAGTACAAACAGGAGAACGCGCGGTTGCGCGAGCTGCTGGGGTCGCCGCTGCGTCAGGACGAGCAGAAAATGGTGACTCAGGTTATTTCAACGGTTAACGATCCTTACAGCGATCAGGTCGTTATCGATAAAGGCAGCGTTAATGGCGTATATGAAGGTCAACCGGTCATTAGCGACAAAGGCGTCGTAGGGCAGGTTGTCGCCGTGGCGAAACTGACCAGTCGCGTACTGCTGATTTGCGATGCGACTCATGCGCTGCCGATTCAGGTACTGCGCAATGACATCCGCGTGATTGCCGCCGGTAACGGCTGCACTGACGATCTTCAGCTTGAACATCTGCCCGCCAATACCGATATTCGCGTTGGCGATGTGCTGGTAACGTCCGGACTTGGTGGGCGTTTCCCGGAAGGGTATCCGGTGGCGGTGGTCTCCTCCGTCAAGCTGGACACTCAGCGCGCCTATACCGTCATTCAGGCACGTCCAACCGCGGGTTTGCAGCGTTTACGCTATCTGCTGCTGTTGTGGGGAGCCGATCGTAACGGCGCCAATCCGATGACGCCGGAAGAGGTTCACCGCGTCGCCAATGAGCGCCTGATGCAGATGATGCCGCAGGTGTTGCCTTCTCCGGATGCGATGGGGCCGCCGGCGCCAGTGCCGGACCCGGCGACGGGGATTACTCAGCCATCTGCGGGCCAGACGGCGCCGGTATCAACGCAACCATCGCCTTCGGGCGCGACCACGCCGCCTGCGCGTGCGCCGGGAGGG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,407 total letters Query= IDP10173.102 Length=1371 Score E Sequences producing significant alignments: (Bits) Value 2_12E_E6_IDP10173.102_T7term.seq 1936 0.0 0_wt 1743 0.0 1_06E_E6_IDP10173.102_T7S.seq 1663 0.0 T T H Y R G I V S G * Q F P S R N N F V * L * E G D I H Query_1 20 ATACGACTC-ACTATAGGGGAATTGTGAG-CGGATAACAATTCCCCT-CTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACA-T 105 2 1235 .....N...C......A..N.NN-.....G......GNNN.-...N.-.......AN....G--..N....--...N.AG.....N..A. 1153 1 21 .......T........................................-. 69 M H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q S Query_1 106 ATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCC 195 2 1125 ............TN.. 1110 2 1152 ..TGNN............... 1132 1 70 .....................------------------------------------------------..................... 111 N A D S R L G T F S Q I R T Y M D T A V S P F Y F I S N G Query_1 196 AATGCCG-ACAGCCGCCTGGGGACGTTCAGTCAAATCCGAACGTACATGGACACTGCCGTCAGTCCTTTCTATTTTA-TTTCAAACGGCC 283 2 1109 .......N......N.......N...-.......N..........................................N............ 1021 0 1 .-.....................................................................-............ 82 1 112 .......-.....................................................................-............ 199 P R E L L D S V S Q T L A S R D Q L E L E N R A L R Q E L L Query_1 284 CTCGTGAACTGCTCGACAGCGTGTCGCAAACGCTGGCTTCTCGCGACCAGCTTGAGCTGGAAAATCGGGCGCTGCGCCAGGAATTACTGC 373 2 1020 .......................................................................................... 931 0 83 .......................................................................................... 172 1 200 .......................................................................................... 289 L K N S D L L M L G Q Y K Q E N A R L R E L L G S P L R Q D Query_1 374 TAAAAAACAGCG-ACCTGCTGATGCTGGGGCAGTACAAACAGGAGAACGCGCGGTTGCGCGAGCTGCTGGGGTCGCCGCTGCGTCAGGAC 462 2 930 ............G............................................................................. 841 0 173 ............-............................................................................. 261 1 290 ............-............................................................................. 378 E Q K M V T Q V I S T V N D P Y S D Q V V I D K G S V N G V Query_1 463 GAGCAGAAAATGGTGACTCAGGTTATTTCAACGGTTAACGATCCTTACAGCGATCAGGTCGTTATCGATAAAGGCAGCGTTAATGGCGTA 552 2 840 .......................................................................................... 751 0 262 .......................................................................................... 351 1 379 .......................................................................................... 468 Y E G Q P V I S D K G V V G Q V V A V A K L T S R V L L I C Query_1 553 TATGAAGGTCAACCGGTCATTAGCGACAAAGGCGTCGTAGGGCAGGTTGTCGCCGTGGCGAAACTGACCAGTCGCGTACTGCTGATTTGC 642 2 750 .......................................................................................... 661 0 352 .......................................................................................... 441 1 469 .......................................................................................... 558 D A T H A L P I Q V L R N D I R V I A A G N G C T D D L Q L Query_1 643 GATGCGACTCATGCGCTGCCGATTCAGGTACTGCGCAATGACATCCGCGTGATTGCCGCCGGTAACGGCTGCACTGACGATCTTCAGCTT 732 2 660 .......................................................................................... 571 0 442 .......................................................................................... 531 1 559 .......................................................................................... 648 E H L P A N T D I R V G D V L V T S G L G G R F P E G Y P V Query_1 733 GAACATCTGCCCGCCAATACCGATATTCGCGTTGGCGATGTGCTGGTAACGTCCGGACTTGGTGGGCGTTTCCCGGAAGGGTATCCGGTG 822 2 570 .......................................................................................... 481 0 532 .......................................................................................... 621 1 649 .......................................................................................... 738 A V V S S V K L D T Q R A Y T V I Q A R P T A G L Q R L R Y Query_1 823 GCGGTGGTCTCCTCCGTCAAGCTGGACACTCAGCGCGCCTATACCGTCATTCAGGCACGTCCAACCGCGGGTTTGCAGCGTTTACGCTAT 912 2 480 .......................................................................................... 391 0 622 .......................................................................................... 711 1 739 .......................................................................................... 828 L L L L W G A D R N G A N P M T P E E V H R V A N E R L M Q Query_1 913 CTGCTGCTGTTGTGGGGAGCCGATCGTAACGGCGCCAATCCGATGACGCCGGAAGAGGTTCACCGCGTCGCCAATGAGCGCCTGATGCAG 1002 2 390 .......................................................................................... 301 0 712 .......................................................................................... 801 1 829 ....................................C..........N..............N..........T.......T........ 918 M M P Q V L P S P D A M G P P A P V P D P A T G I T Q P Query_1 1003 ATGATGCCGCAGGTGTTGCC-TTCTCCGGA-TGCGAT-GGGGCCGCCGGCGCCAGTGCCGGACCCGGCGAC-GGGGATTACTCAGCC--A 1086 2 300 ....................-.........-......-.................................-...............--. 217 0 802 ....................-.........-......-.................................-...............--. 885 1 919 ....................T.........T......G.....NC........N.....N...........G...............CNT 1008 S A G Q T A P V S T Q P S P S G A T T P P A R A P G Query_1 1087 TCTGCGGGCCAGACGG--CGCCGG-TATCAAC-GCAA-CCATCG-CCTTC-GGGCGCGAC-CACGCCGCC--TGCGCG-TGCGCCGGGA- 1164 2 216 ................--......-.......-....-......-.....-.........-.........--......-..........- 139 0 886 ................--......-.......-....-......-.....-.........-.........--......-..........- 963 1 1009 ................GCG.....T.N.NN..N.N..T.N....N....NG...N.T..NT.....N...CG......N..........N 1098 G * H W K W I T D P N S S S V D K L A A A L E H H H H H Query_1 1165 GGGTAACATT--GGAAGTGGATA-ACGG-ATCCGAA-TTCGAG-CTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccac 1248 2 138 ..........--...........-....-.......-......-.............................................. 55 0 964 ... 966 1 1099 ..........TG.A.........N....A....N..T..G...T...... 1148 H * D P A A N K A R Query_1 1249 cacTGAGATCCGGCTGCTAACAAAGCCCGAAA 1280 2 54 ..................N............. 23 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4551445 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 3,407 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP10199.102 (IDP10199.1-201.F..pMCSG53)
MPFTPLVTAGIETALNTFLWRDKALKPARQRLLGKVLRVQLQDFATPVVLVFSERQVDVLSAWDGEADCTVITRLSVLPKLRNRQQLTALIRSGDLEVQGDLQVVQNMVSLCDLAEFDPAELLAPYTGDIVAEGVGKVLRGGAQFLLKGAERQQRYVAEAITEEWRLAPGPLELAWFAEETTAIERALAALEKRLETLEGK
ATGCCTTTCACACCCCTGGTTACCGCTGGCATTGAAACGGCGCTGAATACTTTCCTCTGGCGCGACAAAGCGCTTAAACCCGCTCGCCAGCGCCTGTTGGGCAAGGTATTGCGGGTCCAGCTGCAGGATTTCGCCACGCCCGTGGTGCTGGTATTCAGCGAACGCCAGGTTGACGTCCTGAGCGCCTGGGACGGAGAGGCCGATTGTACCGTGATTACCCGGCTCAGCGTGCTGCCAAAACTGCGCAATCGTCAGCAGCTCACGGCGCTTATTCGCAGCGGTGATCTGGAAGTGCAGGGCGATCTGCAGGTGGTGCAGAATATGGTCTCCCTGTGCGACCTGGCAGAGTTTGATCCCGCTGAACTGCTGGCGCCCTATACCGGCGATATTGTGGCCGAAGGCGTCGGTAAAGTCCTGCGCGGCGGCGCTCAGTTTCTGCTGAAGGGCGCAGAACGCCAGCAGCGCTATGTCGCGGAAGCGATCACTGAAGAGTGGCGGCTGGCGCCGGGGCCGCTGGAGCTGGCCTGGTTTGCTGAAGAGACAACGGCTATTGAGCGCGCGCTGGCCGCACTGGAAAAACGGCTGGAAACGCTGGAGGGCAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,703 total letters Query= IDP10199.102 Length=1008 Score E Sequences producing significant alignments: (Bits) Value 1_02F_F2_IDP10199.102_T7S.seq 1489 0.0 2_08F_F2_IDP10199.102_T7term.seq 1404 0.0 0_wt 1088 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L Query_1 1 TCTCGATCCCGCGAAA-TTAATACG-ACTCACTATAGG-GGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTT 87 1 19 .....T.......N................ 48 2 895 ................A...N....N....NN......N................................................... 806 2 825 ...G...-........T.T.C.C.C........ 856 * E G D I H M H H H H H H S S G V D L W S H P Q F E K G T Query_1 88 TAAG-AAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGA 176 1 49 ....-...................................------------------------------------------------.. 89 2 805 ....G...................................------------------------------------------------.. 764 E N L Y F Q S N A M P F T P L V T A G I E T A L N T F L W R Query_1 177 GAACCTGTACTTCCAATCCAATGCCATGCCTTTCACACCCCTGGTTACCGCTGGCATTGAAACGGCGCTGAATACTTT-CCTCTGGCGCG 265 1 90 ..............................................................................-........... 178 2 763 ..........................N...................................................T........... 674 0 1 .....................................................-........... 64 D K A L K P A R Q R L L G K V L R V Q L Q D F A T P V V L V Query_1 266 ACAAAGCGCTTAAACCCGCTCGCCAGCGCCTGTTGGGCAAGGTATTGCGGGTCCAGCTGCAGGATTTCGCCACGCCCGTGGTGCTGGTAT 355 1 179 ................................C......................................................... 268 2 673 ...........................N....C......................................................... 584 0 65 .......................................................................................... 154 F S E R Q V D V L S A W D G E A D C T V I T R L S V L P K L Query_1 356 TCAGCGAACGCCAGGTTGACGTCCTGAGCGCCTGGGACGGAGAGGCCGATTGTACCGTGATTACCCGGCTCAGCGTGCTGCCAAAACTGC 445 1 269 .....................................A..............C..................................... 358 2 583 .....................................A..............C..................................... 494 0 155 .......................................................................................... 244 R N R Q Q L T A L I R S G D L E V Q G D L Q V V Q N M V S L Query_1 446 GCAATCGTCAGCAGCTCACGGCGCTTATTCGCAGCGGTGATCTGGAAGTGCAGGGCGATCTGCAGGTGGTGCAGAATATGGTCTCCCTGT 535 1 359 .......................................................................................... 448 2 493 .......................................................................................... 404 0 245 .......................................................................................... 334 C D L A E F D P A E L L A P Y T G D I V A E G V G K V L R G Query_1 536 GCGACCTGGCAGAGTTTGATCCCGCTGAACTGCTGGCGCCCTATACCGGCGATATTGTGGCCGAAGGCGTCGGTAAAGTCCTGCGCGGCG 625 1 449 .......................................................................................... 538 2 403 .......................................................................................... 314 0 335 .......................................................................................... 424 G A Q F L L K G A E R Q Q R Y V A E A I T E E W R L A P G P Query_1 626 GCGCTCAGTTTCTGCTGAAGGGCGCAGAACGCCAGCAGCGCTATGTCGCGGAAGCGATCACTGAAGAGTGGCGGCTGGCGCCGGGGCCGC 715 1 539 ............................G............................................C................ 628 2 313 ............................G............................................C................ 224 0 425 .......................................................................................... 514 L E L A W F A E E T T A I E R A L A A L E K R L E T L E G K Query_1 716 TGGAGCTGGCCTGGTTTGCTGAAGAGACAACGGCTATTGAGCGCGCGCTGGCCGCACTGGAAAAACGGCTGGAAACGCTGGAGGGCAAAT 805 1 629 .......................................................................................... 718 2 223 .......................................................................................... 134 0 515 ......................................................................................... 603 * H W K W I T D P N S S S V D K L A A A L E H H H H H H * D Query_1 806 AACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATC 895 1 719 .......................................................................................... 808 2 133 .......................................................................................... 44 P A A N K A R K E A E L A A A T A E Q * L A * P L G A S K R Query_1 896 CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGG 985 1 809 ......................................................-..T................................ 897 2 43 ...................... 22 V L R G F L L K Query_1 986 TC-TTGAGGGGTTTTTTGCTGAAA 1008 1 898 ..T..................... 921 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2639394 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 2,703 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP10220.102 (IDP10220.29-350.P(350)..pMCSG53)
DSRLGTFSQIRTYMDTAVSPFYFISNGPRELLDSVSQTLASRDKLELENRALRQELLLKNSDLLMLGQYKQENARLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQVVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGDVLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNGANPMTPEEVHRVANERLMQMMPQVLPPPDAMGPPAPVPDPATGITQPSAGQTAPVSTQPSPSGATTPPARAPGG
GACAGCCGCCTGGGGACGTTCAGTCAAATCCGAACGTACATGGACACTGCCGTCAGTCCTTTCTATTTTATTTCAAACGGCCCTCGTGAACTGCTCGACAGCGTGTCGCAAACGCTGGCTTCTCGCGACAAGCTTGAGCTGGAAAACCGGGCGCTGCGCCAGGAATTACTGCTAAAAAACAGCGACCTGCTGATGCTGGGGCAGTACAAACAGGAGAACGCGCGGTTGCGCGAGCTGCTGGGGTCGCCGCTGCGTCAGGACGAGCAGAAAATGGTGACGCAGGTTATTTCAACGGTTAACGATCCTTACAGCGATCAGGTCGTTATCGATAAAGGCAGCGTTAATGGCGTATATGAAGGTCAACCGGTCATTAGCGACAAAGGCGTCGTAGGACAGGTTGTCGCCGTGGCGAAACTGACCAGCCGCGTACTGCTGATTTGCGATGCGACTCATGCGCTGCCGATTCAGGTACTGCGCAATGACATCCGCGTGATTGCCGCCGGTAACGGCTGCACTGACGATCTTCAGCTTGAACATCTGCCCGCCAATACCGATATTCGCGTTGGCGATGTGCTGGTAACGTCCGGACTTGGTGGGCGTTTCCCGGAAGGGTATCCGGTGGCGGTGGTCTCCTCCGTCAAGCTGGACACTCAGCGCGCCTATACCGTCATTCAGGCTCGTCCAACCGCAGGTTTGCAGCGTTTGCGCTATCTGCTGCTGTTGTGGGGAGCCGATCGTAACGGTGCTAATCCGATGACGCCGGAAGAGGTTCACCGCGTCGCCAATGAGCGCCTGATGCAGATGATGCCGCAGGTGTTGCCTCCTCCGGATGCGATGGGGCCGCCGGCGCCAGTGCCGGACCCGGCGACGGGGATTACTCAGCCATCTGCGGGCCAGACTGCGCCGGTATCAACGCAACCATCGCCTTCGGGCGCGACCACGCCGCCTGCGCGTGCGCCGGGAGGG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,496 total letters Query= IDP10220.102 Length=1371 Score E Sequences producing significant alignments: (Bits) Value 2_12D_D6_IDP10220.102_T7term.seq 1939 0.0 1_06D_D6_IDP10220.102_T7S.seq 1795 0.0 0_wt 1743 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 60 CCCCTCTAGAAA-TAATTTTGTTTAACTTTAAGAAGGAG--ATATACA-TATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGT 145 2 1191 ...N.......A..N....N....C--...N.......GA.......N.....NN----............. 1126 1 21 ............A..........................--.......-......................------------------- 88 W S H P Q F E K G T E N L Y F Q S N A D S R L G T F S Q I R Query_1 146 GGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGACAGCCGCCTGGGGACGTTCAGTCAAATCCGAA 235 2 1106 .....-....................NN.-.............. 1065 1 89 -----------------------------............................................................. 149 0 1 .................................. 34 T Y M D T A V S P F Y F I S N G P R E L L D S V S Q T L A S Query_1 236 CGTACATGGACACTGCCGTCAGTCCTTTCTATTTTATTT-CAAACGGCCCTCGTGAACTGCTCGACAGCGTGTCGCAAACGCTGGCTTCT 324 2 1064 .......................................T.................................................. 975 2 391 ........... 401 1 150 .......................................-.................................................. 238 1 822 ........... 812 0 35 .......................................-.................................................. 123 0 707 ........... 697 R D K L E L E N R A L R Q E L L L K N S D L L M L G Q Y K Q Query_1 325 CGCGACAAGCTTGAGCTGGAAAACCGGGCGCTGCGCCAGGAATTACTGCTAAAAAACAGCGACCTGCTGATGCTGGGGCAGTACAAACAG 414 2 974 .......................................................................................... 885 2 90 ........... 80 1 239 .......................................................................................... 328 0 124 .......................................................................................... 213 E N A R L R E L L G S P L R Q D E Q K M V T Q V I S T V N D Query_1 415 GAGAACGCGCGGTTGCGCGAGCTGCTGGGGTCGCCGCTGCGTCAGGACGAGCAGAAAATGGTGACGCAGGTTATTTCAACGGTTAACGAT 504 2 884 .......................................................................................... 795 1 329 .......................................................................................... 418 0 214 .......................................................................................... 303 P Y S D Q V V I D K G S V N G V Y E G Q P V I S D K G V V G Query_1 505 CCTTACAGCGATCAGGTCGTTATCGATAAAGGCAGCGTTAATGGCGTATATGAAGGTCAACCGGTCATTAGCGACAAAGGCGTCGTAGGA 594 2 794 .......................................................................................... 705 1 419 .......................................................................................... 508 0 304 .......................................................................................... 393 Q V V A V A K L T S R V L L I C D A T H A L P I Q V L R N D Query_1 595 CAGGTTGTCGCCGTGGCGAAACTGACCAGCCGCGTACTGCTGATTTGCGATGCGACTCATGCGCTGCCGATTCAGGTACTGCGCAATGAC 684 2 704 .......................................................................................... 615 1 509 .......................................................................................... 598 0 394 .......................................................................................... 483 I R V I A A G N G C T D D L Q L E H L P A N T D I R V G D V Query_1 685 ATCCGCGTGATTGCCGCCGGTAACGGCTGCACTGACGATCTTCAGCTTGAACATCTGCCCGCCAATACCGATATTCGCGTTGGCGATGTG 774 2 614 .......................................................................................... 525 1 599 .......................................................................................... 688 0 484 .......................................................................................... 573 L V T S G L G G R F P E G Y P V A V V S S V K L D T Q R A Y Query_1 775 CTGGTAACGTCCGGACTTGGTGGGCGTTTCCCGGAAGGGTATCCGGTGGCGGTGGTCTCCTCCGTCAAGCTGGACACTCAGCGCGCCTAT 864 2 524 .......................................................................................... 435 1 689 .......................................................................................... 778 0 574 .......................................................................................... 663 T V I Q A R P T A G L Q R L R Y L L L L W G A D R N G A N P Query_1 865 ACCGTCATTCAGGCTCGTCCAACCGCAGGTTTGCAGCGTTTGCGCTATCTGCTGCTGTTGTGGGGAGCCGATCGTAACGGTGCTAATCCG 954 2 434 .......................................................................................... 345 2 981 ........... 991 1 779 .......................................................................................... 868 1 232 ........... 222 0 664 .......................................................................................... 753 0 117 ........... 107 M T P E E V H R V A N E R L M Q M M P Q V L P P P D A M G Query_1 955 ATGACGCCGGAAGAGGTTCACCGCGTCGCCAATGAGCGCCTGATGCAG-ATGATGCCGCAGGTGTTGCCT-CCTCCGGATGCGATGGGGC 1042 2 344 ................................................-.....................-................... 257 1 869 ................................................A.....................N................... 958 0 754 ................................................-.....................-................... 841 P P A P V P D P A T G I T Q P S A G Q T A P V S T Q P S P Query_1 1043 CGCCGGCGCCAGTGCCGGACCCGGCGACGGGGATTACTCAGCCATCTGCGGGCCAGACTGCGCCGGTATCAACGCAACCA-TCGCC-TTC 1130 2 256 ................................................................................-.....-... 169 1 959 ...............................................................................NT.....T... 1048 0 842 ................................................................................-.....-... 929 S G A T T P P A R A P G G * H W K W I T D P N S S S V Query_1 1131 GGGCGCGACCA-CGCCGCCTGCGCGTGCGCC-GGGAGGGT-AACATTGG-AAGTGG--ATAACGGAT--CCGAATTCGAGCTCCGTCG-A 1211 2 168 ...........-...................-........-........-......--.........--...................-. 88 2 972 ..-. 970 1 1049 ........A..C......TG...........G.......NA.......NA......GAN........TC....N.N.N..........A. 1138 1 241 ..-. 243 0 930 ...........-...................-....... 966 0 126 ..-. 128 D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 1212 CAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAG 1285 2 87 .....................................................................A.... 14 2 969 ........ 962 1 1139 ....... 1145 1 244 ........ 251 0 129 ........ 136 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4672040 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 3,496 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP10302.102 (IDP10302.1-272.F..pMCSG53)
MSRHSNTPATDASVLLVQLSDSHLFAEDGARLLGMDTAHSLEKVVERVAREQPRIDLILATGDVSQDGSLDSYTRFRRLSAPLDAPLRWFAGNHDEREPMQRATEGSDLLEQVVDVGNWRVVLLDSSIPGAVPGYLEEDQLELLRRAIDSAGERFLLVSFHHHPVPIGSDWMDPIGLRNPQALFDLLAPYPQVRCLLWGHIHQEFDRQRGPLRLLASPSTCVQFAPGSSDFTLDRLAPGYRWLRLHDDGRLETGISRVDDVVFEVDYDTAGY
TTGTCACGCCATTCGAACACTCCCGCCACCGACGCCTCCGTACTGCTGGTGCAGCTCTCGGACAGCCACCTGTTCGCCGAGGATGGCGCGCGCCTGCTTGGCATGGATACCGCCCATAGCCTGGAGAAGGTGGTCGAGCGGGTCGCCCGGGAGCAGCCGCGGATCGACCTGATTCTCGCCACCGGCGACGTCTCCCAGGACGGCAGCCTCGATTCCTACACACGCTTCCGCAGGTTGAGCGCCCCCCTGGACGCGCCGTTGCGCTGGTTCGCCGGTAACCACGACGAACGCGAGCCGATGCAACGGGCGACCGAGGGCAGCGACCTGTTGGAGCAGGTCGTCGACGTCGGCAACTGGCGCGTGGTGTTGCTCGACTCGAGCATTCCCGGCGCGGTGCCGGGCTATCTCGAGGAGGATCAGCTGGAGCTGCTGCGGCGCGCCATCGACAGCGCCGGCGAACGCTTCCTGCTGGTTTCCTTCCATCACCACCCGGTGCCGATCGGCAGCGACTGGATGGATCCCATCGGCCTGCGCAACCCGCAGGCGCTGTTCGACCTGCTGGCGCCCTATCCGCAGGTACGCTGCCTGCTGTGGGGGCACATTCACCAGGAGTTCGACCGCCAGCGCGGCCCGCTGCGCCTGCTGGCTTCGCCGTCCACCTGCGTACAGTTCGCTCCCGGCAGCAGCGACTTCACCCTCGACCGCCTGGCGCCGGGCTACCGCTGGTTGCGCCTGCACGACGACGGGCGCCTGGAAACGGGGATCTCGCGGGTCGACGACGTCGTCTTCGAAGTGGACTACGACACCGCCGGATAC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,327 total letters Query= IDP10302.102 Length=1221 Score E Sequences producing significant alignments: (Bits) Value 2_09D_D3_IDP10302.102_T7term.seq 1804 0.0 1_03D_D3_IDP10302.102_T7S.seq 1608 0.0 0_wt 1472 0.0 R E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 8 CCCGCGAAATTAATACGACTCACTAT-AGGGGAA-TTGTGA-GCGGATAACAATT-CCCCTCTAGAAATAATTTTGTTT-AACTTTAAGA 92 2 1099 ..........................T....N..A......N.............T.......................-.......... 1011 1 21 ...........-...........T.......... 53 E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E N Query_1 93 AGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCT 182 2 1010 ...............N..................------------------------------------------------........ 969 1 54 ..................................------------------------------------------------........ 95 L Y F Q S N A L S R H S N T P A T D A S V L L V Q L S D S H Query_1 183 GTACTTCCAATCCAATGCCTTGTCACGCCATTCGAA-CACTCCCGCCACCGACGCCTCCGTACTGCTGGTGCAGCTCTCGGACAGCCACC 271 2 968 ......T.C...........................A..................................................... 879 1 96 ....................................-..................................................... 184 0 1 .................-..................................................... 70 L F A E D G A R L L G M D T A H S L E K V V E R V A R E Q P Query_1 272 TGTTCGCCGAGGATGGCGCGCGCCTGCTTGGCATGGATACCGCCCATAGCCTGGAGAAGGTGGTCGAGCGGGTCGCCCGGGAGCAGCCGC 361 2 878 .......................................................................................... 789 1 185 .......................................................................................... 274 0 71 .......................................................................................... 160 R I D L I L A T G D V S Q D G S L D S Y T R F R R L S A P L Query_1 362 GGATCGACCTGATTCTCGCCACCGGCGACGTCTCCCAGGACGGCAGCCTCGATTCCTACACACGCTTCCGCAGGTTGAGCGCCCCCCTGG 451 2 788 .......................................................................................... 699 1 275 .......................................................................................... 364 0 161 .......................................................................................... 250 D A P L R W F A G N H D E R E P M Q R A T E G S D L L E Q V Query_1 452 ACGCGCCGTTGCGCTGGTTCGCCGGTAACCACGACGAACGCGAGCCGATGCAACGGGCGACCGAGGGCAGCGACCTGTTGGAGCAGGTCG 541 2 698 .......................................................................................... 609 1 365 .......................................................................................... 454 0 251 .......................................................................................... 340 V D V G N W R V V L L D S S I P G A V P G Y L E E D Q L E L Query_1 542 TCGACGTCGGCAACTGGCGCGTGGTGTTGCTCGACTCGAGCATTCCCGGCGCGGTGCCGGGCTATCTCGAGGAGGATCAGCTGGAGCTGC 631 2 608 .......................................................................................... 519 1 455 .......................................................................................... 544 0 341 .......................................................................................... 430 L R R A I D S A G E R F L L V S F H H H P V P I G S D W M Query_1 632 TGCGGCGCGCCATCGACAGCGCCGGCGAACGCTTCCTGCTGGTTTCCTTCCATCACCACCCGGTGCCGATCGGCAGCGACTG--GATGGA 719 2 518 ..................................................................................--...... 431 2 431 ......--..GT..C............C..GG..GT...... 470 1 545 ..................................................................................--...... 632 1 632 ......--..GT..C............C..GG..GT...... 593 0 431 ..................................................................................--...... 518 0 518 ......--..GT..C............C..GG..GT...... 479 D P I G L R N P Q A L F D L L A P Y P Q V R C L L W G H I H Query_1 720 TCCCATCGGCCTGCGCAACCCGCAGGCGCTGTTCGACCTGCTGGCGCCCTATCCGCAGGTACGCTGCCTGCTGTGGGGGCACATTCACCA 809 2 430 .......................................................................................... 341 1 633 .......................................................................................... 722 0 519 .......................................................................................... 608 Q E F D R Q R G P L R L L A S P S T C V Q F A P G S S D F T Query_1 810 GGAGTTCGACCGCCAGCGCGGCCCGCTGCGCCTGCTGGCTTCGCCGTCCACCTGCGTACAGTTCGCTCCCGGCAGCAGCGACTTCACCCT 899 2 340 .......................................................................................... 251 1 723 ..............-N........................................................G....N............ 811 0 609 .......................................................................................... 698 L D R L A P G Y R W L R L H D D G R L E T G I S R V D D V Query_1 900 CGACCGCCTGGCGCC-GGGCTACCGCTGGTTGCGCC-TGCACGACGACGGGCGCCTGGAAACGGGGATCTCGCGGGTCGACGACGT-CGT 986 2 250 ...............-....................-.................................................-... 164 2 163 ........-... 173 1 812 .N.........N...G.............N......T............................................N..N.N... 901 0 699 ...............-....................-.................................................-... 785 0 786 ........-... 776 V F E V D Y D T A G Y * H W K W I T D P N S S S V D K L A Query_1 987 C-TTCGAAGTGGACTACGACA-CCGCCGGATACTAACATTGGAAGTGGATAACGGAT-CCGAATTCGAGCTCCGTCGACAAGCTTGCGGC 1073 2 163 .-...................-...................................-................................ 77 2 174 . 174 1 902 .T...N..N............N..N...NTAN.....................N...N..A..........N...............N.N 991 0 786 .-...................-........... 816 0 775 . 775 A A L E H H H H H H * D P A A N K A R K E A E L A A A T Query_1 1074 CGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGC-TAACAAAGCCC-GAAAGG--AAGCTG-AGTTGGCTGCT-GCCACCGC 1157 2 76 ..........................................-...........-....A.--... 15 1 992 .N.......N..........CA..-........N....N...T.......N...T......GA.G....A......T....G.G.N.... 1080 A E Q * L A * P L G A S K R V L R G F Query_1 1158 TG-AGCAATAA--CTAGCATAACCCCTT--GGGGCCTCTAAA---CGGGTCTT-GAGGGGTTTTTT 1214 1 1081 ..N.A......ACNA.NA........C.GG.......N....ACNG.......G......A..... 1146 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3951195 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 3,327 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP10305.102 (IDP10305.1-144.F..pMCSG53)
MSTHIQRSALLPYPARALFDLVNDVKRYPEFLPWCSASQVLEESESLMRAELTVAKGSLSQRFTTRNVLVPGASIEMNLENGPFTELHGVWQFKALGEKACKITLDLTFDYAGPLVKATLGPLFTQAANTMVDAFCQRAKQLYG
ATGAGCACCCATATCCAGCGTTCGGCGCTGTTGCCTTATCCCGCCCGCGCGCTGTTCGATTTGGTGAACGACGTGAAGCGCTATCCGGAGTTCCTGCCCTGGTGTTCGGCCAGCCAGGTCCTGGAGGAGAGCGAAAGCCTGATGCGCGCCGAGCTGACCGTGGCCAAGGGTAGCCTCAGCCAGCGCTTCACCACCCGCAACGTGTTGGTGCCGGGAGCTTCCATCGAGATGAACCTGGAAAACGGACCGTTCACCGAACTGCATGGCGTCTGGCAGTTCAAGGCGCTTGGCGAGAAGGCCTGCAAGATCACCCTCGACCTGACCTTCGATTACGCCGGGCCGCTGGTCAAGGCGACCCTCGGGCCGCTGTTCACCCAGGCCGCCAACACCATGGTCGACGCGTTCTGCCAGCGCGCCAAGCAACTCTATGGC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,188 total letters Query= IDP10305.102 Length=837 Score E Sequences producing significant alignments: (Bits) Value 2_09E_E3_IDP10305.102_T7term.seq 1148 0.0 1_03E_E3_IDP10305.102_T7S.seq 1122 0.0 0_wt 780 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTC-TAGAAATAATTTTGTTTAACTTTA 89 2 721 .................................................................-........................ 633 2 654 ...G...........T.T.C.C.C...........A-... 692 1 15 ..T........C........................ 50 * E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E Query_1 90 AGAAGGAGATATACATAT-GCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGA 178 2 632 ..................-...................------------------------------------------------.... 592 1 51 ..................T...................------------------------------------------------.... 92 N L Y F Q S N A M S T H I Q R S A L L P Y P A R A L F D L V Query_1 179 ACCTGTACTTCCAATCCAATGCCATGAGCACCCATATCCAGCGTTCGGCGCTGTTGCCTTATCCCGCCCGCGCGCTGTTCGATTTGGTGA 268 2 591 ..............................T........................................................... 502 1 93 ..............................T........................................................... 182 0 1 ................................................................... 67 N D V K R Y P E F L P W C S A S Q V L E E S E S L M R A E L Query_1 269 ACGACGTGAAGCGCTATCCGGAGTTCCTGCCCTGGTGTTCGGCCAGCCAGGTCCTGGAGGAGAGCGAAAGCCTGATGCGCGCCGAGCTGA 358 2 501 .......................................................................................... 412 1 183 .......................................................................................... 272 0 68 .......................................................................................... 157 T V A K G S L S Q R F T T R N V L V P G A S I E M N L E N G Query_1 359 CCGTGGCCAAGGGTAGCCTCAGCCAGCGCTTCACCACCCGCAACGTGTTGGTGCCGGGAGCTTCCATCGAGATGAACCTGGAAAACGGAC 448 2 411 .......................................................................................... 322 1 273 .......................................................................................... 362 0 158 .......................................................................................... 247 P F T E L H G V W Q F K A L G E K A C K I T L D L T F D Y A Query_1 449 CGTTCACCGAACTGCATGGCGTCTGGCAGTTCAAGGCGCTTGGCGAGAAGGCCTGCAAGATCACCCTCGACCTGACCTTCGATTACGCCG 538 2 321 .......................................................................................... 232 1 363 ...........................................................................N.............. 452 0 248 .......................................................................................... 337 G P L V K A T L G P L F T Q A A N T M V D A F C Q R A K Q L Query_1 539 GGCCGCTGGTCAAGGCGACCCTCGGGCCGCTGTTCACCCAGGCCGCCAACACCATGGTCGACGCGTTCTGCCAGCGCGCCAAGCAACTCT 628 2 231 .......................................................................................... 142 1 453 N.........N........................................................................N...... 542 0 338 .......................................................................................... 427 Y G * H W K W I T D P N S S S V D K L A A A L E H H H H H H Query_1 629 ATGGCTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGA-Gcaccaccaccaccaccac 717 2 141 ......................................................................G................... 52 1 543 ....N................................N............-...................-................... 630 0 428 ..... 432 * D P A A N K A R K E A E L A A A T A E Q * L A * P L G Query_1 718 TGA-GATCCGGCTGCTAACAAAGCCCG-AAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACC--CCTTGGGGC 803 2 51 ...G.....................N.A....... 17 1 631 ...-.....................---G................................N..........N....N.CT.T...N..N 716 A S K R V L R G F L L Query_1 804 CTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAA 836 1 717 ........N..N.......A............. 749 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1761546 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 2,188 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP10320.102 (IDP10320.1-196.F..pMCSG53)
MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFLPGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDEDVPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYGEPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM
ATGAACGTGGTGATCCTTGATACTGGCTGCGCCAACCTGAACTCTGTGAAGTCTGCCATTGCGCGCCACGGTTATGAACCCAAAGTCAGCCGTGACCCGGACATTGTGTTGCTGGCCGATAAACTGTTTTTACCCGGCGTCGGCACCGCACAAGCAGCGATGGATCAGGTGCGTGAGCGCGAGCTGTTTGATCTCATCAAAGCCTGTACCCAACCGGTGCTGGGCATCTGCTTAGGGATGCAACTCCTGGGACGACGCAGCGAAGAGAGCAATGGCGTCGACTTGCTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGACTTTGGTCTGCCACTGCCACATATGGGCTGGAACCGCGTTTATCCGCAGGCAGGCAACCGCCTGTTTCAGGGGATTGAAGACGGCGCGTACTTTTACTTTGTTCACAGCTACGCAATGCCAGTCAATCCGTGGACCATCGCCCAGTGTAATTACGGCGAACCGTTCACCGCGGCGGTACAAAAAGATAACTTCTACGGCGTGCAGTTCCACCCGGAGCGTTCTGGTGCCGCTGGCGCTAAGTTGCTGAAAAACTTCCTGGAGATG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,065 total letters Query= IDP10320.102 Length=993 Score E Sequences producing significant alignments: (Bits) Value 1_06G_G6_IDP10320.102_T7S.seq 1512 0.0 2_12G_G6_IDP10320.102_T7term.seq 1451 0.0 0_wt 1061 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAA 90 1 15 ..T................................. 50 2 875 .......................................................................................... 786 2 808 ...G...........T.T.C.C.C...........A... 846 E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E N Query_1 91 GAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAAC 180 1 51 ....................................------------------------------------------------...... 92 2 785 ....................................------------------------------------------------...... 744 L Y F Q S N A M N V V I L D T G C A N L N S V K S A I A R H Query_1 181 CTGTACTTCCAATCCAATGCCATGAACGTGGTGATCCTTGATACTGGCTGCGCCAACCTGAACTCTGTGAAGTCTGCCATTGCGCGCCAC 270 1 93 .......................................................................................... 182 2 743 .......................................................................................... 654 0 1 ..................................................................... 69 G Y E P K V S R D P D I V L L A D K L F L P G V G T A Q A A Query_1 271 GGTTATGAACCCAAAGTCAGCCGTGACCCGGACATTGTGTTGCTGGCCGATAAACTGTTTTTACCCGGCGTCGGCACCGCACAAGCAGCG 360 1 183 .......................................................................................... 272 2 653 .......................................................................................... 564 0 70 .......................................................................................... 159 M D Q V R E R E L F D L I K A C T Q P V L G I C L G M Q L L Query_1 361 ATGGATCAGGTGCGTGAGCGCGAGCTGTTTGATCTCATCAAAGCCTGTACCCAACCGGTGCTGGGCATCTGCTTAGGGATGCAACTCCTG 450 1 273 .......................................................................................... 362 2 563 .......................................................................................... 474 0 160 .......................................................................................... 249 0 284 ........... 294 G R R S E E S N G V D L L G I I D E D V P K M T D F G L P L Query_1 451 GGACGACGCAGCGAAGAGAGCAATGGCGTCGACTTGCTGGGCATCATCGACGAAGACGTGCCGAAAATGACCGACTTTGGTCTGCCACTG 540 1 363 .......................................................................................... 452 1 331 ........... 341 2 473 .......................................................................................... 384 2 505 ........... 495 0 250 .......................................................................................... 339 0 218 ........... 228 P H M G W N R V Y P Q A G N R L F Q G I E D G A Y F Y F V H Query_1 541 CCACATATGGGCTGGAACCGCGTTTATCCGCAGGCAGGCAACCGCCTGTTTCAGGGGATTGAAGACGGCGCGTACTTTTACTTTGTTCAC 630 1 453 .......................................................................................... 542 2 383 .......................................................................................... 294 0 340 .......................................................................................... 429 S Y A M P V N P W T I A Q C N Y G E P F T A A V Q K D N F Y Query_1 631 AGCTACGCAATGCCAGTCAATCCGTGGACCATCGCCCAGTGTAATTACGGCGAACCGTTCACCGCGGCGGTACAAAAAGATAACTTCTAC 720 1 543 .......................................................................................... 632 2 293 .......................................................................................... 204 0 430 .......................................................................................... 519 G V Q F H P E R S G A A G A K L L K N F L E M * H W K W I T Query_1 721 GGCGTGCAGTTCCACCCGGAGCGTTCTGGTGCCGCTGGCGCTAAGTTGCTGAAAAACTTCCTGGAGATGTAACATTGGAAGTGGATAACG 810 1 633 .......................................................................................... 722 2 203 .......................................................................................... 114 0 520 ..................................................................... 588 D P N S S S V D K L A A A L E H H H H H H * D P A A N K A Query_1 811 GATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCG- 899 1 723 .........................................................................................- 811 2 113 .........................................................................................A 24 R K E A E L A A A T A E Q * L A * P L G A S K R V L R G F L Query_1 900 AAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCT 989 1 812 .......................................................................................... 901 2 23 ....... 17 L K Query_1 990 GAAA 993 1 902 .... 905 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2953560 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 3,065 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP10324.102 (IDP10324.1-352.F..pMCSG53)
MTHISERLLVQAHLAAKQPRVLSEQESAEHRAAIAAELKAQNAVLVAHYYCDPVIQALAEETGGCVSDSLEMARFGNQHPAQTVVVAGVRFMGETAKILNPEKRVLMPTLEATCSLDLGCPVDEFSAFCDQHPERTVVVYANTSAAVKARADWVVTSSCAVEIVEHLMDNGEPILWAPDQHLGRYIQRETGADMLLWDGACIVHEEFKAKQLEDMKALYPDAAILVHPESPESVVALADAVGSTSQLIKAAQTLPNKTFIVATDRGIFYKMQQLCPDKDFIEAPTAGNGAACRSCAHCPWMAMNTLERTLACLREGSGEIFVDPALIPRAVRPLKRMLDFTQAARLRQAGNA
ATGACGCACATTTCCGAACGACTCCTGGTACAGGCCCACCTGGCCGCCAAGCAACCCCGTGTGTTGAGCGAGCAGGAGAGCGCCGAGCATCGCGCGGCGATCGCGGCCGAACTGAAGGCGCAAAATGCTGTACTGGTGGCGCATTACTACTGCGACCCGGTGATCCAGGCGTTGGCCGAGGAGACCGGCGGTTGCGTATCCGATTCGCTGGAGATGGCCCGTTTCGGCAACCAGCATCCGGCGCAGACGGTGGTCGTGGCCGGGGTGCGCTTCATGGGCGAGACGGCGAAGATCCTCAACCCTGAGAAGCGTGTGCTGATGCCGACCCTCGAAGCGACCTGCTCGCTCGACCTGGGATGCCCGGTGGATGAATTCTCGGCTTTCTGCGACCAGCACCCGGAACGGACCGTGGTGGTCTATGCGAACACCTCCGCGGCGGTGAAGGCACGCGCCGACTGGGTCGTGACCTCCAGTTGCGCGGTGGAGATCGTCGAACACCTGATGGACAACGGCGAGCCCATCCTCTGGGCGCCGGACCAGCACCTGGGACGCTACATCCAGCGCGAGACGGGGGCCGACATGCTGCTCTGGGATGGCGCCTGTATCGTCCACGAGGAGTTCAAGGCCAAGCAGCTGGAAGACATGAAGGCGCTCTACCCGGACGCCGCCATCCTGGTCCACCCCGAATCGCCGGAAAGCGTGGTCGCGCTGGCCGATGCCGTGGGCTCGACCAGCCAGTTGATCAAGGCCGCGCAGACCCTGCCGAACAAGACCTTCATCGTCGCCACCGATCGCGGCATCTTCTACAAGATGCAGCAGTTGTGCCCGGACAAGGATTTCATCGAGGCCCCCACCGCCGGCAACGGCGCCGCCTGCCGCAGCTGCGCGCACTGCCCGTGGATGGCGATGAACACGCTCGAGCGGACCCTGGCCTGCCTGCGCGAGGGCAGCGGGGAAATCTTCGTCGACCCGGCGTTGATCCCGCGGGCGGTCCGTCCGCTCAAGCGCATGCTCGACTTCACCCAGGCCGCGCGCCTGCGCCAGGCCGGGAACGCC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,202 total letters Query= IDP10324.102 Length=1461 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1905 0.0 2_09F_F3_IDP10324.102_T7term.seq 1831 0.0 1_03F_F3_IDP10324.102_T7S.seq 1590 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 60 CCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTC 149 1 23 ...........-.......................................................----------------------- 88 S H P Q F E K G T E N L Y F Q S N A M T H I S E R L L V Q A Query_1 150 TCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGACGCACATTTCCGAACGACTCCTGGTACAGGCCCA 239 0 1 ...................................... 38 1 89 -------------------------................................................................. 153 H L A A K Q P R V L S E Q E S A E H R A A I A A E L K A Q Query_1 240 CCTGGCCGCCAAGCAACCCCGTGTGTTGAGCGAG-CAGGAGAGCG-CCGA-GCAT-CGCGCGGCGATCGCGGCCGAACTGAAGGCGCAAA 325 0 39 ..................................-..........-....-....-.................................. 124 0 351 ..C.......-.... 338 2 1144 ....T.GA......G..........N....N....T.NNN....A..................-...... 1076 2 841 ..C.......-.... 854 1 154 ..................................-..........-....-....-.................................. 239 1 466 ..C.......-.... 453 N A V L V A H Y Y C D P V I Q A L A E E T G G C V S D S L Query_1 326 ATGCTGTACTGG-TGGCG-CATTACTACTG-CGACCCGGTGATCCAGGCGTTGGCCGAGGAGACCGG-CGGTTGCGTATCCGATTCGCTG 411 0 125 ............-.....-...........-....................................-...................... 210 2 1075 ...-...N....N.....G...........A..N......N.......GCG................A......N............... 987 1 240 ............-.....-...........-....................................-...................... 325 E M A R F G N Q H P A Q T V V V A G V R F M G E T A K I Query_1 412 GAGATGGCCCGTTTCGGCAACCAGCATCC-GGCGCAGA-CGGTGGTCGTGGC-CGGGGTGCGCTTCATGGGCGA-GACGGCGAAGATCCT 497 0 211 .............................-........-.............-.....................-............... 296 2 986 N............................C........A.......N.....N.....................A....A.......... 897 1 326 .............................-........-.............-.....................-............... 411 L N P E K R V L M P T L E A T C S L D L G C P V D E F S A F Query_1 498 CAACCCTGAGAAGCGTGTGCTGATGCCGACCCTCGAA-GCGACCTGCTCGCTCGACCTGGGATGCCCGGTGGATGAATT-CTCGGCTTTC 585 0 297 .....................................-.........................................-.......... 384 0 76 ...........A.. 63 2 896 .....................................A.........................................T.......... 807 1 412 .....................................-.........................................-.......... 499 1 191 ...........A.. 178 C D Q H P E R T V V V Y A N T S A A V K A R A D W V V T S S Query_1 586 TGCGACCAGCACCCGGAACGGACCGTGGTGGTCTATGCGAACACCTCCGCGGCGGTGAAGGCACGCGCCGACTGGGTCGTGACCTCCAGT 675 0 385 .......................................................................................... 474 2 806 .......................................................................................... 717 1 500 .......................................................................................... 589 C A V E I V E H L M D N G E P I L W A P D Q H L G R Y I Q R Query_1 676 TGCGCGGTGGAGATCGTCGAACACCTGATGGACAACGGCGAGCCCATCCTCTGGGCGCCGGACCAGCACCTGGGACGCTACATCCAGCGC 765 0 475 .......................................................................................... 564 2 716 .......................................................................................... 627 1 590 .......................................................................................... 679 E T G A D M L L W D G A C I V H E E F K A K Q L E D M K A L Query_1 766 GAGACGGGGGCCGACATGCTGCTCTGGGATGGCGCCTGTATCGTCCACGAGGAGTTCAAGGCCAAGCAGCTGGAAGACATGAAGGCGCTC 855 0 565 .......................................................................................... 654 2 626 .......................................................................................... 537 1 680 ...............................................................N...................N...... 769 Y P D A A I L V H P E S P E S V V A L A D A V G S T S Q L Query_1 856 TACCCGGACGCCGCCAT-CCTGGTCCACCCCGAATCGCCGGAAAGCGTGGTCGCGCT-GGCCGATGCCGTGGGCTCGACCAGCCAGTTGA 943 0 655 .................-.......................................-................................ 742 2 536 .................-.......................................-................................ 449 1 770 ............N....C.....................N.................N................................ 859 I K A A Q T L P N K T F I V A T D R G I F Y K M Q Q L C P Query_1 944 TCAAGGCCGCGCAGA-CCCTGCCGAACAAGACCTTCATCGTCGCCACCGATCGCGGCATCTTCTACAAGATGCAGCAGTTGTGCCC-GGA 1031 0 743 ...............-......................................................................-... 830 2 448 ...............-......................................................................-... 361 1 860 ......N........C...............................N..........................-...........G... 948 D K D F I E A P T A G N G A A C R S C A H C P W M A M N T L Query_1 1032 CAAGGATTTCATCGAGGCCCCCACCGCCGGCAACGGCGCCGCCTGCCGCAGCTGCGCGCACTGCCCGTGGATGGCGATGAACACGCTCGA 1121 0 831 .......................................................................................... 920 2 360 .......................................................................................... 271 1 949 ..............................N.....N...N.......-.... 1000 E R T L A C L R E G S G E I F V D P A L I P R A V R P L K R Query_1 1122 GCGGACCCTGGCCTGCCTGCGCGAGGGCAGCGGGGAAATCTTCGTCGACCCGGCGTTGATCCCGCGGGCGGTCCGTCCGCTCAAGCGCAT 1211 0 921 .......................................................................................... 1010 2 270 .......................................................................................... 181 M L D F T Q A A R L R Q A G N A * H W K W I T D P N S S S V Query_1 1212 GCTCGACTTCACCCAGGCCGCGCGCCTGCGCCAGGCCGGGAACGCCTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGA 1301 0 1011 .............................................. 1056 2 180 .......................................................................................... 91 D K L A A A L E H H H H H H * D P A A N K A R Query_1 1302 CAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAA 1370 2 90 ..................................................................... 22 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4557530 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 3,202 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP10332.102 (IDP10332.1-347.F..pMCSG53)
MSVMFDPQAAIYPFPPKPTPLNDDEKQFYREKIKRLLKERNAVMVAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHAASTLLVAGVRFMGETAKILSPEKNILMPTLAAECSLDLGCPIDEFSAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAVELIEHLDSLGEKIIWAPDRHLGNYVQKQTGADVLCWQGACIVHDEFKTQALTRLKKIYPDAALLVHPESPQSIVEMADAVGSTSQLIKAAKTLPHRQLIVATDRGIFYKMQQAVPEKELLEAPTAGEGATCRSCAHCPWMAMNGLKAIAEGLEQGGAAHEIQVDAALREGALLPLNRMLDFAATLRA
ATGAGCGTAATGTTTGACCCACAAGCCGCAATCTACCCGTTCCCGCCGAAACCAACGCCGTTGAACGACGACGAAAAGCAATTTTATCGTGAGAAAATCAAGCGGTTGCTCAAAGAGCGCAATGCCGTCATGGTGGCGCACTACTACACCGATCCGGAAATTCAACAGTTAGCAGAAGAAACCGGCGGCTGTATTTCCGACTCGCTGGAAATGGCCCGTTTCGGCGCGAAGCACGCCGCATCCACGTTACTGGTAGCGGGCGTACGGTTTATGGGGGAAACCGCCAAAATCCTCAGCCCGGAAAAAAACATTCTTATGCCTACGCTGGCGGCGGAATGTTCGCTGGATTTAGGCTGCCCAATAGACGAATTTAGCGCTTTTTGTGATGCGCACCCTGACAGAACCGTAGTGGTCTATGCTAACACCTCGGCTGCTGTTAAAGCGCGTGCGGACTGGGTTGTGACCTCCAGTATCGCCGTAGAACTGATTGAGCATCTGGATAGTTTGGGGGAAAAAATTATCTGGGCGCCGGACAGACACCTGGGGAATTACGTGCAAAAACAGACCGGGGCCGATGTACTGTGTTGGCAAGGGGCGTGTATCGTGCATGACGAGTTTAAAACCCAGGCGTTGACCCGTTTAAAAAAAATCTATCCCGATGCCGCTCTTCTGGTTCACCCTGAATCGCCGCAGTCCATTGTCGAGATGGCCGATGCGGTGGGCTCCACCAGTCAGCTTATTAAGGCGGCAAAAACGCTGCCGCACAGACAGCTTATCGTGGCGACCGATCGCGGCATCTTCTACAAAATGCAGCAGGCGGTGCCTGAAAAAGAGCTGCTTGAAGCGCCCACGGCTGGCGAGGGGGCGACCTGCCGTAGCTGCGCGCACTGTCCGTGGATGGCGATGAATGGCCTGAAAGCCATTGCTGAAGGGCTGGAGCAGGGAGGCGCGGCGCATGAAATACAGGTTGATGCGGCGCTACGCGAGGGCGCATTACTGCCCCTCAACCGGATGCTGGATTTTGCGGCTACACTTCGGGCG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,504 total letters Query= IDP10332.102 Length=1446 Score E Sequences producing significant alignments: (Bits) Value 2_10E_E4_IDP10332.102_T7term.seq 1903 0.0 0_wt 1878 0.0 1_04E_E4_IDP10332.102_T7S.seq 1855 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 57 ATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTG 146 1 16 ......................................................................-------------------- 85 W S H P Q F E K G T E N L Y F Q S N A M S V M F D P Q A A Query_1 147 GTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAA--TCCAATGCCATGAGCG---TAATGTTTGACCCACAAGCCGCA 231 2 1196 .......NT...........N..A.NGG...N--......AC.....GN.. 1148 0 1 .......---....................... 30 1 86 ----------------------------..................--................---....................... 142 I Y P F P P K P T P L N D D E K Q F Y R E K I K R L L K Query_1 232 ATCTACCCGTTCCCGCCG-AAACCAACGCCGTTGAACGACGACGAAAAGCAATTTT-ATCGTGAGAAAATCAAGCGGTTGCTC--AAAGA 317 2 1147 T.NN...-...N......G..........-.......NNG--..............TN.......N................TCA..... 1062 0 31 ..................-.....................................-..........................--..... 116 1 143 ..................-.....................................-..........................--..... 228 E R N A V M V A H Y Y T D P E I Q Q L A E E T G G C I S D Query_1 318 GCGCAATGCCGTCATGGTGGCGC-ACTACTACACCGAT-CCGG-AAATTCAACAGTTAGCAGAAGAAACCGGCGGCTGTATTTCCGACTC 404 2 1061 .......................N.N.N..........T....N.............................................. 972 0 117 .......................-..............-....-.............................................. 203 1 229 .......................-..............-....-.............................................. 315 S L E M A R F G A K H A A S T L L V A G V R F M G E T A K I Query_1 405 GCTGGAAATGGCCCGTTTCGG-CGCGAAGCACGCCGCATCCACGTTACTGGTAGCGGGCGTACGGTTTATGGGGGAAACCGCCAAAATCC 493 2 971 ............A........G....................................................--.............. 884 0 204 .....................-.................................................................... 292 1 316 .....................-.................................................................... 404 L S P E K N I L M P T L A A E C S L D L G C P I D E F S A F Query_1 494 TCAGCCCGGaaaaaaaCATTCTTATGCCTACGCTGGCGGCGGAATGTTCGCTGGATTTAGGCTGCCCAATAGACGAATTTAGCGCTTTTT 583 2 883 ...........................-.............................................................. 795 0 293 .......................................................................................... 382 1 405 .......................................................................................... 494 C D A H P D R T V V V Y A N T S A A V K A R A D W V V T S S Query_1 584 GTGATGCGCACCCTGACAGAACCGTAGTGGTCTATGCTAACACCTCGGCTGCTGTTAAAGCGCGTGCGGACTGGGTTGTGACCTCCAGTA 673 2 794 .......................................................................................... 705 0 383 .......................................................................................... 472 1 495 .......................................................................................... 584 I A V E L I E H L D S L G E K I I W A P D R H L G N Y V Q K Query_1 674 TCGCCGTAGAACTGATTGAGCATCTGGATAGTTTGGGGGAAAAAATTATCTGGGCGCCGGACAGACACCTGGGGAATTACGTGCAAAAAC 763 2 704 .......................................................................................... 615 0 473 .......................................................................................... 562 1 585 .......................................................................................... 674 Q T G A D V L C W Q G A C I V H D E F K T Q A L T R L K K I Query_1 764 AGACCGGGGCCGATGTACTGTGTTGGCAAGGGGCGTGTATCGTGCATGACGAGTTTAAAACCCAGGCGTTGACCCGTTTaaaaaaaaTCT 853 2 614 .......................................................................................... 525 0 563 .......................................................................................... 652 1 675 .......................................................................................... 764 Y P D A A L L V H P E S P Q S I V E M A D A V G S T S Q L I Query_1 854 ATCCCGATGCCGCTCTTCTGGTTCACCCTGAATCGCCGCAGTCCATTGTCGAGATGGCCGATGCGGTGGGCTCCACCAGTCAGCTTATTA 943 2 524 .......................................................................................... 435 0 653 .......................................................................................... 742 1 765 .......................................................................................... 854 K A A K T L P H R Q L I V A T D R G I F Y K M Q Q A V P E K Query_1 944 AGGCGGCAAAAACGCTGCCGCACAGACAGCTTATCGTGGCGACCGATCGCGGCATCTTCTACAAAATGCAGCAGGCGGTGCCTGAAAAAG 1033 2 434 .......................................................................................... 345 0 743 .......................................................................................... 832 1 855 .................................................................T........................ 944 E L L E A P T A G E G A T C R S C A H C P W M A M N G L K A Query_1 1034 AGCTGCTTGAAGCGCCCACGGCTGGCGAGGGGGCGACCTGCCGTAGCTGCGCGCACTGTCCGTGGATGGCGATGAATGGCCTGAAAGCCA 1123 2 344 .......................................................................................... 255 0 833 .......................................................................................... 922 1 945 ...............................................................-..N............-.......... 1032 I A E G L E Q G G A A H E I Q V D A A L R E G A L L P L N Query_1 1124 TTGCTGAAGGGC-TGGAGCAGGGAGGCGCGGCGCAT-GAAATACAGGTTG-ATGCGGCGCTACGCGAGGG-CGCATTACTGCCCCTCAAC 1209 2 254 ............-.......................-.............-...................-................... 169 0 923 ............-.......................-.............-...................-................... 1008 1 1033 ........T...T....NN.-........N......N.............A............N.N....NN............T..-.. 1120 R M L D F A A T L R A * H W K W I T D P N S S S V D K L A Query_1 1210 CGGATGCTGGATTTTGCGGCTACACTTCGGGCGTAACATTGGAA-GTG-GATAACGG-ATCCGAATTCGAGCTCCGTCGACAAGCTTGCG 1296 2 168 ............................................-...-........-................................ 82 0 1009 ................................. 1041 1 1121 .......N.......N.............---NNT.......N.G...AA.......T...... 1181 A A L E H H H H H H * D P A A N K A R K E Query_1 1297 GCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAG-CCCGAAAGGAA 1359 2 81 ....................................................C.......A... 18 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4942080 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 3,504 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP10343.102 (IDP10343.1-275.F..pMCSG53)
MESLLTLPLAGEARVRILQITDTHLFAEKHETLLGVNTWESYQAVLEAIRAQQYEYDLIVATGDLAQDQSAAAYQHFAEGIASFRAPCVWLPGNHDFQPAMYSALQEAGISPAKRVLIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERQTLLLLHHHPLPAGCSWLDQHSLRNAGELDSVLANFPCVKYLLCGHIHQELDLDWNGRRLLASPSTCVQFKPHCANFTLDTIAPGWRTLELQANGVLETEVHRLQDTRFRPDTASEGY
TTGGAAAGCCTGTTAACCCTACCTCTGGCTGGTGAGGCCCGAGTCAGGATTTTACAAATTACAGATACTCACCTGTTTGCCGAAAAGCATGAAACGCTGTTAGGCGTCAATACCTGGGAAAGTTACCAGGCCGTGCTGGAGGCTATTCGGGCGCAACAGTATGAATATGATCTGATCGTGGCCACCGGCGATCTGGCGCAGGATCAATCCGCTGCGGCTTATCAGCATTTTGCTGAGGGTATCGCAAGCTTTCGCGCGCCTTGCGTTTGGCTACCGGGCAATCATGATTTTCAGCCGGCAATGTACAGCGCGCTGCAAGAGGCGGGGATTTCCCCGGCTAAACGCGTGTTAATCGGTGAACAATGGCAGATTTTGTTGCTGGATAGCCAGGTGTTTGGCGTCCCGCATGGCGAACTGAGCGAGTTCCAACTGGAATGGCTTGAGCGTAAGCTCGCCGATGCGCCAGAGAGACAGACATTGCTCCTGTTGCACCACCATCCGTTGCCGGCAGGGTGCAGTTGGCTGGATCAGCACAGCTTGCGTAATGCTGGTGAGCTGGATAGCGTGCTGGCGAATTTCCCGTGTGTAAAATATCTGTTATGCGGGCATATTCACCAGGAGCTGGATCTTGACTGGAACGGTCGCCGTCTGTTGGCGTCGCCGTCAACCTGCGTACAGTTCAAACCGCACTGCGCGAACTTTACGCTGGATACTATCGCGCCGGGCTGGCGTACGCTGGAGCTCCAGGCGAACGGCGTCCTGGAGACGGAAGTGCATCGCCTGCAGGATACCCGGTTCCGACCCGATACCGCTTCGGAAGGATAC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,327 total letters Query= IDP10343.102 Length=1230 Score E Sequences producing significant alignments: (Bits) Value 2_10F_F4_IDP10343.102_T7term.seq 1716 0.0 1_04F_F4_IDP10343.102_T7S.seq 1685 0.0 0_wt 1489 0.0 S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * E Query_1 7 TCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCT-CTAGAAA-TAATTTTGTTTAACTTTAAGAAG 94 2 1114 ..............NNN.NN..N..N.........-...........C..T.......T..N....A............NN..A.GA... 1026 1 17 ........T..N....-....................... 55 G D I H M H H H H H H S S G V D L W S H P Q F E K G T E N L Query_1 95 GA-GATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTG 183 2 1025 ..N.N...........................N.-----------------------------------------------......... 983 1 56 ..-..............................------------------------------------------------......... 96 Y F Q S N A L E S L L T L P L A G E A R V R I L Q I T D Query_1 184 TACTTCCAA-TCCAATGCC-TTGGAAA-GCCTGTTAACCCTACCTCTGGCTGG-TGAGGCCCGAGTCAGGATTTTACAAATTACAGATAC 269 2 982 .........N..A...N..C.......A.........................G....................-............... 894 1 97 .........-.........-.......-.........................-.................................... 182 0 1 .......-.........................-.................................... 68 T H L F A E K H E T L L G V N T W E S Y Q A V L E A I R A Q Query_1 270 TCACCTGTTTGCCGAAAAGCATGAAACGCTGTTAGGCGTCAATACCTGGGAAAGTTACCAGGCCGTGCTGGAGGCTATTCGGGCGCAACA 359 2 893 .........-...............................................A................................ 805 1 183 .......................................................................................... 272 0 69 .......................................................................................... 158 Q Y E Y D L I V A T G D L A Q D Q S A A A Y Q H F A E G I A Query_1 360 GTATGAATATGATCTGATCGTGGCCACCGGCGATCTGGCGCAGGATCAATCCGCTGCGGCTTATCAGCATTTTGCTGAGGGTATCGCAAG 449 2 804 .......................................................................................... 715 1 273 .......................................................................................... 362 0 159 .......................................................................................... 248 S F R A P C V W L P G N H D F Q P A M Y S A L Q E A G I S P Query_1 450 CTTTCGCGCGCCTTGCGTTTGGCTACCGGGCAATCATGATTTTCAGCCGGCAATGTACAGCGCGCTGCAAGAGGCGGGGATTTCCCCGGC 539 2 714 .......................................................................................... 625 1 363 .......................................................................................... 452 0 249 .......................................................................................... 338 A K R V L I G E Q W Q I L L L D S Q V F G V P H G E L S E F Query_1 540 TAAACGCGTGTTAATCGGTGAACAATGGCAGATTTTGTTGCTGGATAGCCAGGTGTTTGGCGTCCCGCATGGCGAACTGAGCGAGTTCCA 629 2 624 .......................................................................................... 535 1 453 .......................................................................................... 542 0 339 .......................................................................................... 428 Q L E W L E R K L A D A P E R Q T L L L L H H H P L P A G C Query_1 630 ACTGGAATGGCTTGAGCGTAAGCTCGCCGATGCGCCAGAGAGACAGACATTGCTCCTGTTGCACCACCATCCGTTGCCGGCAGGGTGCAG 719 2 534 .......................................................................................... 445 1 543 .......................................................................................... 632 0 429 .......................................................................................... 518 S W L D Q H S L R N A G E L D S V L A N F P C V K Y L L C G Query_1 720 TTGGCTGGATCAGCACAGCTTGCGTAATGCTGGTGAGCTGGATAGCGTGCTGGCGAATTTCCCGTGTGTAAAATATCTGTTATGCGGGCA 809 2 444 .......................................................................................... 355 1 633 .......................................................................................... 722 0 519 .......................................................................................... 608 H I H Q E L D L D W N G R R L L A S P S T C V Q F K P H C A Query_1 810 TATTCACCAGGAGCTGGATCTTGACTGGAACGGTCGCCGTCTGTTGGCGTCG-CCGTCAACCTGCGTACAGTTCAAA-CCGCACTGCGCG 897 2 354 ....................................................-........................-............ 267 1 723 .........N..........................................C........................C............ 812 0 609 ....................................................-........................-............ 696 N F T L D T I A P G W R T L E L Q A N G V L E T E V H R L Query_1 898 AACTTTACGCTGGATAC-TATCGCGCCGGGCTGGCGTACGCTGG-AGC-TCCAGGCGAACGGCGTCCTGGAGACGGAAGTGCATCGCCTG 984 2 266 .................-..........................-...-......................................... 180 2 216 ....-...-..... 227 1 813 .................T..........................N...T.......N................................. 902 0 697 .................-..........................-...-......................................... 783 0 747 ....-...-..... 736 Q D T R F R P D T A S E G Y * H W K W I T D P N S S S V D Query_1 985 CAGGATACCCGGTTCCGACCCGATACCGCTTCGGAAGGATACTAACATTGGAAGTGGATAACGGATCCGAATTCGAGC-TCCGTCGACAA 1073 2 179 ..............................................................................-........... 91 1 903 ..N.....N.....................C..A.................-..N.....C.................T......T.-T. 990 0 784 .......................................... 825 K L A A A L E H H H H H H * D P A A N K A R K E A E L A A A Query_1 1074 GCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTG-AGATCCGGCTGCTAAC-AAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCC 1161 2 90 .......................................-................A........... 24 1 991 ................N.N........-.N.........N.N.....T........-...............N....N...T.....N.. 1078 T A E Q * L A * P L G A S K R V L R G F L L K Query_1 1162 ACCGCTGAGCAATAACTAGCATA--ACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGG--TTTTTTGC-TGAAA 1230 1 1079 ......N..N..NT....ATC..NNN...N.GNN.........--C.NN..N......NCN.......T..... 1150 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3980706 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 3,327 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP95844.102 (IDP95844.1-345.F..pMCSG53)
MFKKLRGMFSSDLSIDLGTANTLIYVRERGIVLNEPSVVAIRSHGSQKSVVAVGTEAKRMLGRTPGNIAAIRPMKDGVIADFSVCEKMLQYFINKVHENSFLQPSPRVLICVPCKSTQVERRAIRESALGAGAREVFLIEEPMAAAIGAGLPVEEARGSMVVDIGGGTTEIALISLNGVVYAESVRVGGDRFDEAIVTYVRRNYGSLIGESTAERIKQEIGTAFPGGDVREVDVRGRNLAEGVPRSFTLNSNEVLEALQESLATIVQAVKSALEQSPPELASDIAERGLVLTGGGALLRDLDKLLAQETGLPVIVAEEPLTCVARGGGRALEMMDRHSMDLLSTE
ATGTTCAAAAAATTGCGTGGCATGTTTTCCAGCGATCTGTCGATCGACCTGGGTACTGCCAATACCCTTATTTATGTGCGCGAGCGCGGCATCGTCCTGAACGAACCGTCCGTGGTCGCCATTCGCAGCCATGGCAGCCAGAAGAGCGTCGTCGCCGTGGGCACCGAGGCCAAGCGCATGCTCGGCCGTACCCCGGGCAACATCGCCGCCATCCGTCCGATGAAAGACGGCGTGATCGCGGATTTCAGCGTCTGCGAGAAGATGCTGCAGTACTTCATCAACAAGGTCCACGAGAACAGCTTCCTGCAGCCCAGCCCACGCGTGCTGATCTGCGTGCCGTGCAAGTCCACCCAGGTTGAGCGCCGGGCCATCCGCGAATCCGCCCTGGGCGCCGGCGCCCGCGAGGTGTTCCTGATCGAAGAGCCGATGGCTGCCGCCATCGGCGCCGGCCTGCCGGTCGAGGAGGCCCGCGGCTCGATGGTCGTAGACATCGGCGGCGGCACTACCGAGATCGCCCTGATTTCCCTCAACGGTGTTGTCTACGCCGAATCCGTCCGCGTCGGCGGCGACCGCTTCGACGAAGCCATCGTCACCTACGTGCGCCGCAACTACGGCAGCCTGATCGGCGAATCCACCGCCGAGCGCATCAAGCAGGAGATCGGCACCGCCTTCCCGGGCGGCGACGTTCGCGAAGTCGACGTCCGCGGCCGCAACCTGGCCGAAGGCGTACCGCGCAGCTTCACCCTGAACTCCAACGAGGTCCTCGAGGCCCTGCAGGAGTCCCTGGCGACCATCGTCCAGGCGGTCAAGAGCGCCCTCGAGCAATCGCCGCCGGAGCTGGCCTCGGATATCGCCGAGCGCGGCCTGGTGCTGACCGGCGGTGGCGCGCTGCTGCGCGACCTGGACAAGCTGCTGGCACAGGAAACCGGCCTGCCGGTGATCGTCGCCGAGGAGCCGCTGACCTGCGTGGCTCGCGGCGGTGGCCGTGCGCTGGAGATGATGGACCGCCACTCCATGGACCTGCTCTCCACCGAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,467 total letters Query= IDP95844.102 Length=1440 Score E Sequences producing significant alignments: (Bits) Value 2_09G_G3_IDP95844.102_T7term.seq 1898 0.0 0_wt 1867 0.0 1_03G_G3_IDP95844.102_T7S.seq 1770 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 60 CCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTC 149 1 20 ...........-.......................................................----------------------- 85 S H P Q F E K G T E N L Y F Q S N A M F K K L R G M F S S D Query_1 150 TCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGTTCAAAAAATTGCGTGG-CATGTTTTCCAGCGAT- 237 2 1169 .......G......G..N.N.NNNAG.....T 1138 0 1 ....................-................- 36 1 86 -------------------------...............................................-................- 148 L S I D L G T A N T L I Y V R E R G I V L N E P S V V A I Query_1 238 CTGTCGATCGA-CCTGGGTACTGCCAATACCCTTATTTATGTGCGCG-AGCGCGGCATCGTCCTGAACGAACCGTCCGTGGTCG-CCATT 324 2 1137 ...........A.N.........N....NN.......ANGNN.....N............N.N-.......NN.....N.-...N..... 1050 0 37 ...........-...................................-....................................-..... 123 1 149 ...........-...................................-....................................-..... 235 R S H G S Q K S V V A V G T E A K R M L G R T P G N I A A Query_1 325 CGCAGCCATGGCAGCCAGAAGAGCGTCGTCGCCGTGGGCACCGA-GGCCAAGCGCATGCTCGGCCGTACCCCGGGCAACATCGCCGCCAT 413 2 1049 ....N.......................................N......................N...................... 960 0 124 ............................................-............................................. 212 1 236 ............................................-............................................. 324 1 960 ....-........ 949 I R P M K D G V I A D F S V C E K M L Q Y F I N K V H E N S Query_1 414 CCGTCCGATGAAAGACGGCGTGATCGCGGATTTCAGCGTCTGCGAGAAGATGCTGCAGTACTTCATCAACAAGGTCCACGAGAACAGCTT 503 2 959 ................................-......................................................... 871 0 213 .......................................................................................... 302 1 325 .......................................................................................... 414 F L Q P S P R V L I C V P C K S T Q V E R R A I R E S A L G Query_1 504 CCTGCAGCCCAGCCCACGCGTGCTGATCTGCGTGCCGTGCAAGTCCACCCAGGTTGAGCGCCGGGCCATCCGCGAATCCGCCCTGGGCGC 593 2 870 .......................................................................................... 781 2 773 ...... 778 0 303 .......................................................................................... 392 0 400 ...... 395 1 415 .......................................................................................... 504 1 512 ...... 507 A G A R E V F L I E E P M A A A I G A G L P V E E A R G S M Query_1 594 CGGCGCCCGCGAGGTGTTCCTGATCGAAGAGCCGATGGCTGCCGCCATCGGCGCCGGCCTGCCGGTCGAGGAGGCCCGCGGCTCGATGGT 683 2 780 .......................................................................................... 691 2 779 ........ 786 2 247 ............. 235 0 393 .......................................................................................... 482 0 394 ........ 387 0 926 ............. 938 1 505 .......................................................................................... 594 1 506 ........ 499 1 1041 ............ 1052 V V D I G G G T T E I A L I S L N G V V Y A E S V R V G G D Query_1 684 CGTAGACATCGGCGGCGGCACTACCGAGATCGCCCTGATTTCCCTCAACGGTGTTGTCTACGCCGAATCCGTCCGCGTCGGCGGCGACCG 773 2 690 .......................................................................................... 601 0 483 .......................................................................................... 572 1 595 .......................................................................................... 684 R F D E A I V T Y V R R N Y G S L I G E S T A E R I K Q E I Query_1 774 CTTCGACGAAGCCATCGTCACCTACGTGCGCCGCAACTACGGCAGCCTGATCGGCGAATCCACCGCCGAGCGCATCAAGCAGGAGATCGG 863 2 600 .......................................................................................... 511 0 573 .......................................................................................... 662 1 685 .......................................................................................... 774 G T A F P G G D V R E V D V R G R N L A E G V P R S F T L N Query_1 864 CACCGCCTTCCCGGGCGGCGACGTTCGCGAAGTCGACGTCCGCGGCCGCAACCTGGCCGAAGGCGTACCGCGCAGCTTCACCCTGAACTC 953 2 510 .......................................................................................... 421 0 663 .......................................................................................... 752 1 775 .......................................................................................... 864 S N E V L E A L Q E S L A T I V Q A V K S A L E Q S P P E L Query_1 954 CAACGAGGTCCTCGAGGCCCTGCAGGAGTCCCTGGCGACCATCGTCCAGGCGGTCAAGAGCGCCCTCGAGCAATCGCCGCCGGAGC-TGG 1042 2 420 ......................................................................................-... 332 0 753 ......................................................................................-... 841 1 865 ......................................................................................T... 954 A S D I A E R G L V L T G G G A L L R D L D K L L A Q E T Query_1 1043 CCTCGGATATCGCCGAGCGCGGCCTGGTGCTGACCGGCGGTGGCGCGCTGC-TGCGCGACCTGGACAAGCT-GCTGGCACAGGAAACCGG 1130 2 331 ...................................................-...................-.................. 244 2 727 .... 724 0 842 ...................................................-...................-.................. 929 0 446 .... 449 1 955 .......N...........................................T...........T.......G..........A..N.... 1044 1 558 .... 561 G L P V I V A E E P L T C V A R G G G R A L E M M D R H S Query_1 1131 CCTGCCGGTGATCGTCGCCGAGGAGCCGC-TGACC-TGCGTGGCTCGCGGCGGTGGCCGTGCGCTGGAG-ATGATGGACCG-CCACTCCA 1216 2 243 .............................-.....-.................................-...........-........ 158 2 723 ......... 715 0 930 .............................-.....-.................................-...........-........ 1015 0 450 ......... 458 1 1045 ........N....N...N.A.........T.....N...N.-.NN.NN...........N.NN.....NA....AT.N...C...TN..N 1133 1 562 ......... 570 M D L L S T E * H W K W I T D P N S S S V D K L A A A L E Query_1 1217 -TGGACCTGC-TCTCCACCGAGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGca 1304 2 157 -.........-............................................................N.................. 70 0 1016 -.........-........... 1035 1 1134 T..A......N..N..... 1152 H H H H H H * D P A A N K A R K E Query_1 1305 ccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCC--GAAAGGAA 1353 2 69 .........................................CNA....... 19 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4868656 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 3,467 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96244.102 (IDP96244.21-211.P(211)..pMCSG53)
AADQSNPYKLMNEAAQKTFDRLKNEQPKIRANPDYLRDVVDQELLPYVQVKYAGALVLGRYYKEATPAQREAYFAAFREYLKQAYGQALAMYHGQTYQIAPEQPLGDATIVPIRVTIIDPNGRPPVRLDFQWRKNTQTGNWQAYDMIAEGVSMITTKQNEWSDLLRTKGIDGLTAQLKSISQQKITLDEKQ
GCAGCGGATCAGAGCAATCCTTATAAACTGATGAACGAGGCGGCGCAGAAAACTTTTGATCGCCTGAAAAACGAACAGCCGAAAATTCGTGCCAATCCGGATTACCTGCGCGACGTTGTTGATCAGGAACTTCTGCCGTACGTGCAGGTAAAATATGCCGGCGCGCTGGTGTTGGGCCGCTATTACAAAGAGGCGACTCCGGCGCAGCGTGAAGCCTATTTTGCCGCGTTTCGCGAATACCTGAAACAGGCATACGGTCAGGCGCTGGCGATGTACCACGGCCAGACTTACCAGATAGCGCCTGAGCAACCGCTGGGCGATGCGACCATTGTGCCTATCCGTGTAACGATTATCGATCCCAACGGTCGTCCGCCGGTACGCCTGGATTTCCAGTGGCGCAAAAACACCCAGACCGGCAACTGGCAGGCGTATGACATGATTGCGGAAGGCGTCAGCATGATCACCACCAAGCAAAATGAGTGGAGCGATCTGCTGCGTACTAAAGGCATCGACGGCCTGACCGCACAACTGAAATCTATCTCTCAACAGAAAATTACTTTGGACGAGAAACAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,081 total letters Query= IDP96244.102 Length=978 Score E Sequences producing significant alignments: (Bits) Value 1_05E_E5_IDP96244.102_T7S.seq 1463 0.0 2_11E_E5_IDP96244.102_T7term.seq 1413 0.0 0_wt 1034 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAA 90 1 16 ..T.............-................... 50 2 861 .......................................................................................... 772 2 794 ...G...........T.T.C.C.C...........A... 832 E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E N Query_1 91 GAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAAC 180 1 51 ....................................------------------------------------------------...... 92 2 771 ....................................------------------------------------------------...... 730 L Y F Q S N A A A D Q S N P Y K L M N E A A Q K T F D R L Query_1 181 CTGTACTTCCAATCCAATGCCGCAGCGGATCAGAGCAATCCTTATAAA-CTGATGAACGAGGCGGCGCAGAAAACTTTTGATCGCCTGAA 269 1 93 ................................................-......................................... 181 2 729 ................................................A......................................... 640 0 1 ...........................-......................................... 68 K N E Q P K I R A N P D Y L R D V V D Q E L L P Y V Q V K Y Query_1 270 AAACGAACAGCCGAAAATTCGTGCCAATCCGGATTACCTGCGCGACGTTGTTGATCAGGAACTTCTGCCGTACGTGCAGGTAAAATATGC 359 1 182 .......................................................................................... 271 1 916 ............ 905 2 639 .......................................................................................... 550 0 69 .......................................................................................... 158 A G A L V L G R Y Y K E A T P A Q R E A Y F A A F R E Y L K Query_1 360 CGGCGCGCTGGTGTTGGGCCGCTATTACAAAGAGGCGACTCCGGCGCAGCGTGAAGCCTATTTTGCCGCGTTTCGCGAATACCTGAAACA 449 1 272 .......................................................................................... 361 1 969 ........... 979 2 549 .......................................................................................... 460 0 159 .......................................................................................... 248 Q A Y G Q A L A M Y H G Q T Y Q I A P E Q P L G D A T I V P Query_1 450 GGCATACGGTCAGGCGCTGGCGATGTACCACGGCCAGACTTACCAGATAGCGCCTGAGCAACCGCTGGGCGATGCGACCATTGTGCCTAT 539 1 362 .......................................................................................... 451 2 459 .......................................................................................... 370 0 249 .......................................................................................... 338 I R V T I I D P N G R P P V R L D F Q W R K N T Q T G N W Q Query_1 540 CCGTGTAACGATTATCGATCCCAACGGTCGTCCGCCGGTACGCCTGGATTTCCAGTGGCGCAAAAACACCCAGACCGGCAACTGGCAGGC 629 1 452 .......................................................................................... 541 2 369 .......................................................................................... 280 0 339 .......................................................................................... 428 A Y D M I A E G V S M I T T K Q N E W S D L L R T K G I D G Query_1 630 GTATGACATGATTGCGGAAGGCGTCAGCATGATCACCACCAAGCAAAATGAGTGGAGCGATCTGCTGCGTACTAAAGGCATCGACGGCCT 719 1 542 .......................................................................................... 631 2 279 .......................................................................................... 190 0 429 .......................................................................................... 518 L T A Q L K S I S Q Q K I T L D E K Q * H W K W I T D P N S Query_1 720 GACCGCACAACTGAAATCTATCTCTCAACAGAAAATTACTTTGGACGAGAAACAGTAACATTGGAAGTGGATAACGGATCCGAATTCGAG 809 1 632 .......................................................................................... 721 2 189 .......................................................................................... 100 0 519 ....................................................... 573 S S V D K L A A A L E H H H H H H * D P A A N K A R K E A E Query_1 810 CTCCGTCGACAAGCTTGCGGCCGCACTCGAGcacca-ccaccaccaccacTGAGATCCGGCTGCTAACAAAG-CCCGAAAGGAAGCTGAG 897 1 722 ....................................C...................................-................. 810 2 99 ....................................-...................................C......... 19 L A A A T A E Q * L A * P L G A S K R V L R G F L L K Query_1 898 TTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTT-GGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAA 978 1 811 ..........................................G....................................... 892 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2923668 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 3,081 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96361.102 (IDP96361.24-134.P(134)..pMCSG53)
NDHKILGVIAMPRNETNDLALKIPVCRIVKRIQLTADHGDIELSGASVYFKTARSASQSLNVPSSIKEGQTTGWININSDNDNKRCVSKITFSGHTVNSSDMARLKVIGDD
AACGACCATAAAATACTGGGTGTTATTGCCATGCCCAGAAATGAAACGAACGATCTCGCGCTGAAAATTCCCGTCTGCCGCATCGTGAAGCGCATTCAACTCACGGCGGATCACGGCGATATCGAACTTAGCGGCGCATCCGTCTATTTTAAAACTGCGCGTAGCGCCAGCCAAAGCCTGAACGTCCCTTCCTCAATCAAAGAAGGCCAGACGACAGGCTGGATCAATATTAATAGCGATAACGACAACAAACGCTGCGTATCAAAGATTACCTTCTCAGGTCATACGGTAAATTCTTCCGATATGGCGAGACTTAAAGTCATTGGCGACGAT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,531 total letters Query= IDP96361.102 Length=738 Score E Sequences producing significant alignments: (Bits) Value 1_05F_F5_IDP96361.102_T7S.seq 1025 0.0 2_11F_F5_IDP96361.102_T7term.seq 975 0.0 0_wt 601 1e-175 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTC-TAGAAATAATTTTGTTTAACTTTA 89 1 19 ........CN....N.................. 51 2 621 .................................................................-........................ 533 2 554 ...G...........T.T.C.C.C...........A-... 592 * E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E Query_1 90 AGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAA 179 1 52 .....................................------------------------------------------------..... 93 2 532 .....................................------------------------------------------------..... 491 N L Y F Q S N A N D H K I L G V I A M P R N E T N D L A L K Query_1 180 CCTGTACTTCCAATCCAATGCCAACGACCATAAAATACTGGGTGTTATTGCCATGCCCAGAAATGAAACGAACGATCTCGCGCTGAAAAT 269 1 94 .......................................................................................... 183 2 490 .......................................................................................... 401 0 1 .................................................................... 68 I P V C R I V K R I Q L T A D H G D I E L S G A S V Y F K T Query_1 270 TCCCGTCTGCCGCATCGTGAAGCGCATTCAACTCACGGCGGATCACGGCGATATCGAACTTAGCGGCGCATCCGTCTATTTTAAAACTGC 359 1 184 .......................................................................................... 273 1 695 ........... 705 2 400 .......................................................................................... 311 0 69 .......................................................................................... 158 A R S A S Q S L N V P S S I K E G Q T T G W I N I N S D N D Query_1 360 GCGTAGCGCCAGCCAAAGCCTGAACGTCCCTTCCTCAATCAAAGAAGGCCAGACGACAGGCTGGATCAATATTAATAGCGATAACGACAA 449 1 274 .......................................................................................... 363 2 310 .......................................................................................... 221 0 159 .......................................................................................... 248 N K R C V S K I T F S G H T V N S S D M A R L K V I G D D * Query_1 450 CAAACGCTGCGTATCAAAGATTACCTTCTCAGGTCATACGGTAAATTCTTCCGATATGGCGAGACTTAAAGTCATTGGCGACGATTAACA 539 1 364 .......................................................................................... 453 2 220 .......................................................................................... 131 0 249 ..................................................................................... 333 H W K W I T D P N S S S V D K L A A A L E H H H H H H * D P Query_1 540 TTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGC 629 1 454 .......................................................................................... 543 2 130 .......................................................................................... 41 A A N K A R K E A E L A A A T A E Q * L A * P L G A S K R Query_1 630 TGCTAA--CAAAGCCCG-AAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGT 716 1 544 ......--.........-........................................................................ 630 2 40 ......ACA.......NA....... 16 V L R G F L L K Query_1 717 C-TTGAGGGG-TTTTTTGCTGAAA 738 1 631 .T........T............. 654 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1797378 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 2,531 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96368.102 (IDP96368.1-272.F..pMCSG53)
MIYIGLPQWSHPKWARLGITSLEEYARHFNCVEGNTTLYALPKAEIVDRWYAQTTDDFRFCFKFPATISHQAALRHCDDLVQAFFTRLAPLETRIGQYWLQLPAAFGPRDLPALWQFLDALPATFTYGVEVRHPCFFDKGEDEQRLNRGLHARGVNRVILDSRPVHAAHPHSEAVRDAQRKKPKVPVHAVVTASHPMVRFIGSDNMAQNREFFAAWLQKLPQWRQTTTPFLFLHTPDIAQAPELVNTLWHDLRSVLPEIGTAPSIPQQSSLF
ATGATCTACATTGGCTTGCCGCAATGGTCGCACCCGAAATGGGCGCGGCTCGGCATCACCAGTCTGGAAGAGTATGCCCGCCACTTTAACTGCGTGGAGGGCAACACCACGCTTTACGCGCTGCCGAAAGCGGAAATTGTCGATCGCTGGTATGCGCAAACTACGGACGATTTCCGTTTTTGCTTTAAATTTCCCGCCACCATTTCTCACCAGGCCGCTCTGCGCCATTGCGACGATCTGGTACAGGCGTTTTTTACCCGCCTGGCGCCGCTGGAAACGCGTATTGGCCAATACTGGCTGCAGTTGCCCGCCGCCTTTGGACCGCGCGATCTCCCCGCCTTATGGCAGTTTCTTGATGCGCTCCCCGCCACGTTTACCTATGGCGTAGAAGTTCGTCATCCGTGTTTTTTTGACAAGGGTGAGGATGAACAGCGGCTCAATCGCGGGTTACACGCGCGCGGCGTAAACCGCGTGATCCTGGATAGTCGTCCGGTACATGCCGCCCATCCCCACAGCGAGGCCGTCCGTGACGCGCAGCGAAAAAAACCCAAAGTCCCGGTACATGCGGTCGTGACGGCCAGCCATCCGATGGTGCGTTTTATCGGTAGCGATAATATGGCGCAAAACCGTGAGTTTTTCGCTGCCTGGTTGCAGAAACTCCCGCAGTGGCGGCAGACCACCACGCCTTTTCTGTTTTTACATACTCCGGATATCGCGCAGGCGCCGGAACTGGTGAATACGCTTTGGCATGATTTACGCAGCGTCTTACCGGAAATCGGCACTGCTCCGTCCATTCCACAGCAATCTTCTCTTTTC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,318 total letters Query= IDP96368.102 Length=1221 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1472 0.0 2_10G_G4_IDP96368.102_T7term.seq 203 3e-55 1_04G_G4_IDP96368.102_T7S.seq 113 4e-28 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 58 TTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGG 147 2 368 ..G........... 355 1 20 .....T.......-....................................................... 87 S H P Q F E K G T E N L Y F Q S N A M I Y I G L P Q W S H P Query_1 148 TCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGATCTACATTGGCTTGCCGCAATGGTCGCACCCG 237 0 1 .................................... 36 1 88 ................................ 119 K W A R L G I T S L E E Y A R H F N C V E G N T T L Y A L P Query_1 238 AAATGGGCGCGGCTCGGCATCACCAGTCTGGAAGAGTATGCCCGCCACTTTAACTGCGTGGAGGGCAACACCACGCTTTACGCGCTGCCG 327 0 37 .......................................................................................... 126 2 752 ............ 763 1 940 ............ 929 K A E I V D R W Y A Q T T D D F R F C F K F P A T I S H Q Query_1 328 AAAGCGGAAATTGTCGATCGCTGGTATGCGCAAACTACGGACGATTTCCGTTTTTGCTTTAAATTTCCCGCC-ACCATTTCTCACCAGGC 416 0 127 ........................................................................-................. 215 2 608 ......... 616 1 1107 ...N....G...GN.GG......... 1082 A A L R H C D D L V Q A F F T R L A P L E T R I G Q Y W L Q Query_1 417 CGCTCTGCGCCATTGCGACGATCTGGTACAGGCGTTTTTTACCCGCCTGGCGCCGCTGGAAACGCGTATTGGCCAATACTGGCTGCAGTT 506 0 216 .......................................................................................... 305 0 294 .............. 281 2 617 .. 618 1 1081 .. 1080 L P A A F G P R D L P A L W Q F L D A L P A T F T Y G V E V Query_1 507 GCCCGCCGCCTTTGGACCGCGCGATCTCCCCGCCTTATGGCAGTTTCTTGATGCGCTCCCCGCCACGTTTACCTATGGCGTAGAAGTTCG 596 0 306 .......................................................................................... 395 R H P C F F D K G E D E Q R L N R G L H A R G V N R V I L D Query_1 597 TCATCCGTGtttttttGACAAGGGTGAGGATGAACAGCGGCTCAATCGCGGGTTACACGCGCGCGGCGTAAACCGCGTGATCCTGGATAG 686 0 396 .......................................................................................... 485 S R P V H A A H P H S E A V R D A Q R K K P K V P V H A V V Query_1 687 TCGTCCGGTACATGCCGCCCATCCCCACAGCGAGGCCGTCCGTGACGCGCAGCGaaaaaaaCCCAAAGTCCCGGTACATGCGGTCGTGAC 776 0 486 .......................................................................................... 575 0 490 ........... 500 0 556 ........... 566 2 707 ............ 718 1 985 ............ 974 T A S H P M V R F I G S D N M A Q N R E F F A A W L Q K L P Query_1 777 GGCCAGCCATCCGATGGTGCGTTTTATCGGTAGCGATAATATGGCGCAAAACCGTGAGTTTTTCGCTGCCTGGTTGCAGAAACTCCCGCA 866 0 576 .......................................................................................... 665 0 544 ........... 534 Q W R Q T T T P F L F L H T P D I A Q A P E L V N T L W H D Query_1 867 GTGGCGGCAGACCACCACGCCTTTTCTGTTTTTACATACTCCGGATATCGCGCAGGCGCCGGAACTGGTGAATACGCTTTGGCATGATTT 956 0 666 .......................................................................................... 755 L R S V L P E I G T A P S I P Q Q S S L F * H W K W I T D P Query_1 957 ACGCAGCGTCTTACCGGAAATCGGCACTGCTCCGTCCATTCCACAGCAATCTTCTCTTTTCTAACATTGGAAGTGGATAACGGATCCGAA 1046 0 756 ............................................................. 816 2 136 ............................... 106 N S S S V D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 1047 TTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCC--GAAAGGAA 1134 2 105 ................................................................................CGA....... 16 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3940350 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 3,318 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96372.103 (IDP96372.1-365.P(480)..pMCSG53)
MIMRQTKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTTICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAATRQHTDCDPLMLVLAADHSIANEEAFRDAVRGAMPYADAGKLVTFGIVPDLPETGYGYIRRGDVVPGATDAVAFEVAQFVEKPGLETAQAYVASGDYYWNSGMFLFRAGRYLEELKKFRPDILAACEQAMRGVDPDLDFIRVDEEAFLACPEESIDYAVMERTADAVVMPMDAGWSDVGSWSSLWEISAHTPEGNVHHGDVISHKTENSYVYAESGLVTTVGVKDLVVVQTKDAVLIADRHAVQDVKKVVEKIKADGRHEH
ATGATAATGCGTCAGACAAAACTTTACCCGGTTGTGATGGCCGGTGGCTCCGGTAGCCGATTGTGGCCGCTTTCTCGCGTGCTTTATCCAAAACAGTTTCTGTGCCTGAAAGGCGATCTCACCATGCTGCAAACCACCATTTGCCGTCTGAACGGCGTGGAGTGCGAAAGCCCGGTGGTTATCTGCAACGAACAGCACCGTTTTATCGTTGCCGAGCAGCTCCGCCAGCTCAATAAGCTGACGGAGAACATTATTCTTGAGCCCGCCGGGCGCAATACCGCGCCGGCGATTGCGTTGGCGGCGCTGGCGGCGACGCGCCAGCATACGGACTGCGATCCGCTGATGCTGGTACTGGCGGCGGATCATTCGATCGCCAATGAAGAGGCCTTCCGCGACGCGGTGCGCGGCGCCATGCCCTATGCCGACGCGGGCAAACTGGTGACCTTCGGTATCGTGCCGGATCTGCCGGAAACCGGCTATGGCTATATCCGGCGCGGTGATGTCGTGCCGGGCGCGACGGATGCGGTGGCTTTTGAGGTGGCGCAGTTCGTTGAGAAGCCGGGGCTGGAAACCGCTCAGGCCTACGTCGCCAGCGGCGATTACTACTGGAACAGCGGCATGTTTCTGTTCCGCGCCGGACGCTATCTGGAAGAGCTGAAAAAGTTCCGCCCCGATATTCTGGCCGCCTGTGAACAGGCGATGCGCGGCGTCGATCCGGATCTTGACTTCATTCGCGTGGATGAAGAGGCCTTCCTCGCCTGTCCGGAAGAGTCCATTGACTACGCGGTGATGGAGCGAACGGCGGACGCGGTGGTGATGCCGATGGACGCGGGCTGGAGCGATGTCGGCTCGTGGTCCTCGCTGTGGGAAATCAGCGCCCACACCCCGGAGGGAAACGTCCACCACGGCGACGTCATCAGCCATAAAACGGAAAACAGCTACGTTTACGCCGAATCCGGCCTGGTGACCACCGTCGGCGTGAAGGATCTGGTGGTAGTGCAGACCAAAGACGCCGTGCTGATTGCCGATCGCCACGCGGTGCAGGACGTGAAAAAGGTGGTGGAGAAGATCAAGGCCGACGGACGCCATGAGCAC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,321 total letters Query= IDP96372.103 Length=1500 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1975 0.0 2_11G_G5_IDP96372.103_T7term.seq 1847 0.0 1_05G_G5_IDP96372.103_T7S.seq 1478 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 60 CCCCT-CTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGT 148 1 21 .....N...-.....................................................N....---------------------- 87 S H P Q F E K G T E N L Y F Q S N A M I M R Q T K L Y P V V Query_1 149 CTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGATAATGCGTCAGACAAAACTTTACCCGGTTGTGA 238 0 1 ..................................... 37 1 88 --------------------------................................................................ 151 M A G G S G S R L W P L S R V L Y P K Q F L C L K G D L T Query_1 239 TGGCCGGTGGCTCC-GGTAGCC-GATTGTGGCCGCTTTCT-CGCGTGCTTTATCCAAAACAGTTTCTGTGCCTGAAAGGCGATCTCACCA 325 0 38 ..............-.......-.................-................................................. 124 2 1187 ....A.......N....N.NCAN.......T.N.....A.NNN.A...N.......NA-....TN..A........NN.. 1109 1 152 ..............-.......-.................-................................................. 238 M L Q T T I C R L N G V E C E S P V V I C N E Q H R F I V A Query_1 326 TGCTGC-AAACCACCATTTGCCGTCTGAACGGCGTGGAGTGCGAAAGCCCGGTGGTTATCTGCAACGAACAGCACCGTTTTATCGTTGCC 414 0 125 ......-................................................................................... 213 2 1108 .....NN....NN....NNN.N..N.NNN.NN.........NN.............NN.......N....................AC.G 1019 1 239 ......-................................................................................... 327 E Q L R Q L N K L T E N I I L E P A G R N T A P A I A L A Query_1 415 GAGCAGCTCCGCCAGCTCAATAAGCTGACGGAGAACATTATTCTTGAGCCCGCCGGGCGCAATACCGCGCCGGCGATTGCGTTGG-CGGC 503 0 214 .....................................................................................-.... 302 2 1018 N..................-........................-...............................A..N.....N.... 931 1 328 .....................................................................................-.... 416 A L A A T R Q H T D C D P L M L V L A A D H S I A N E E A F Query_1 504 GCTGGCGGCGACGCGCCAGCATACGG-ACTGCGATCCGCTGATGCTGGTACTGGCGGCGGATCATTCGATCGCCAATGAA-GAGGCCTTC 591 0 303 ..........................-.....................................................-......... 390 0 740 .....-......... 753 2 930 ..........................N.....................................................A......... 841 2 490 .....-......... 477 1 417 ..........................-.....................................................-......... 504 1 862 ..-......... 872 R D A V R G A M P Y A D A G K L V T F G I V P D L P E T G Y Query_1 592 CGCGACGCGGTGCGCGGCGCCATGCCCTATGCCGACGCGGGCAAACTGGTGACCTTCGGTATCGTGCCGGATCTGCCGGAAACCGGCTAT 681 0 391 .......................................................................................... 480 0 754 .T.. 757 2 840 .......................................................................................... 751 2 476 .T.. 473 1 505 .......................................................................................... 594 1 873 .T.. 876 G Y I R R G D V V P G A T D A V A F E V A Q F V E K P G L Query_1 682 GGCTATATCCGGCGCGGTGATGTCGTGCCGGGCGCGACGGATGCGGTGGCTTTTGAGGTGGCGCAGTTCGTTGAGAA-GCCGGGGCTGGA 770 0 481 .............................................................................-............ 569 2 750 .............................................................................A............ 661 1 595 .............................................................................-............ 683 E T A Q A Y V A S G D Y Y W N S G M F L F R A G R Y L E E L Query_1 771 AACCGCTCAGGCCTACGTCGCCAGCGGCGATTACTACTGGAACAGCGGCATGTTTCTGTT-CCGCGCCGGACGCTA-TCTGGAAGAGCTG 858 0 570 ............................................................-...............-............. 657 2 660 ............................................................-...............-............. 573 1 684 .......................................................N....C...............T............. 773 K K F R P D I L A A C E Q A M R G V D P D L D F I R V D Query_1 859 -AAAAAGTTC-CGCCCCGATATTCTGGCCGCCTGTG-AACAGGCGATGCGCGGCGTCGATCCGGATCTTGACTTCATTCGCGTGGATG-A 944 0 658 -.........-.........................-...................................................-. 743 0 377 ...-. 380 2 572 -.........-.........................-...................................................-. 487 2 851 . 851 1 774 A.........N................N........A.......................................-..N........A. 862 1 491 ...-. 494 E E A F L A C P E E S I D Y A V M E R T A D A V V M P M D Query_1 945 AGAGGCCTTCCTCGCCTGTCCGGAAGAGTCCA-TTGACTACGCGGT-GATGGAGCGAACGG-CGGACGCGGTGG-TGATGCCGATGGACG 1030 0 744 ................................-.............-..............-............-............... 829 0 381 ...........G.. 394 2 486 ................................-.N...........-..............-............-............... 401 2 850 ...........G.. 837 1 863 ................................T......T.N....G..N...........N.......N....T............... 952 1 495 ...........G.. 508 A G W S D V G S W S S L W E I S A H T P E G N V H H G D V I Query_1 1031 CGGGCTGGAGCGATGTCGGCTCGTGGTCCTCGCTGTGGGAAATCAGCGCCCACACCCCGGAGGGAAACGTCCACCACGGCGACGTCATCA 1120 0 830 .......................................................................................... 919 2 400 .......................................................................................... 311 1 953 ....N....N..... 967 S H K T E N S Y V Y A E S G L V T T V G V K D L V V V Q T K Query_1 1121 GCCATAAAACGGAAAACAGCTACGTTTACGCCGAATCCGGCCTGGTGACCACCGTCGGCGTGAAGGATCTGGTGGTAGTGCAGACCAAAG 1210 0 920 .......................................................................................... 1009 2 310 .......................................................................................... 221 D A V L I A D R H A V Q D V K K V V E K I K A D G R H E H * Query_1 1211 ACGCCGTGCTGATTGCCGATCGCCACGCGGTGCAGGACGTGAAAAAGGTGGTGGAGAAGATCAAGGCCGACGGACGCCATGAGCACTAAC 1300 0 1010 ...................................................................................... 1095 2 220 .......................................................................................... 131 H W K W I T D P N S S S V D K L A A A L E H H H H H H * D P Query_1 1301 ATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGG 1390 2 130 ...........................................N.............................................. 41 A A N K A R Query_1 1391 CTGCTAACAAAGCCCGAAA 1409 2 40 ................... 22 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4857132 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 3,321 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96598.102 (IDP96598.22-237.P(237)..pMCSG53)
GVTAERTRLVFVEGQREASLLLVNQNPYPVLVQAWVDDGALDGEPDTALAPFLPLPPVFRLEPGRQRSLRLLATGQAQARDRESLYWLNIYEIPPQATAEAAGRPRLTVTLRTQMKVLYRPHGLHPHAEDAAAELGFALRDETLRVDNPTPYFVSLAGLALEIGETRLDLPGTLLAPRSQRRLGLPRRVDGGTRGEVAFDWIDDNGVSRQERKALQ
GGAGTGACCGCGGAGCGCACCCGCCTGGTGTTCGTGGAGGGCCAGCGCGAGGCCTCGTTGCTGCTGGTCAACCAGAATCCCTACCCGGTGCTGGTGCAGGCGTGGGTCGATGACGGCGCCCTCGACGGCGAACCGGATACCGCGCTGGCTCCGTTCCTGCCGCTGCCGCCCGTATTCCGCCTGGAGCCGGGACGCCAGCGCAGCCTGCGCCTGCTGGCTACCGGCCAGGCCCAGGCACGGGATCGCGAATCGCTGTACTGGCTGAACATCTACGAGATCCCCCCGCAAGCCACCGCCGAGGCCGCTGGCCGGCCCCGGCTCACGGTCACCCTGCGAACCCAGATGAAGGTGCTGTACCGCCCGCACGGCCTGCATCCGCACGCCGAGGACGCGGCCGCCGAACTGGGCTTCGCGCTGCGCGACGAGACCCTGCGGGTGGACAACCCGACGCCGTACTTCGTGTCCCTGGCGGGGCTGGCGCTGGAGATAGGGGAGACGCGCCTGGATCTGCCCGGAACGCTGCTGGCTCCGCGCTCGCAACGACGCCTCGGCCTGCCGCGGCGGGTGGACGGCGGGACGCGCGGCGAGGTGGCGTTCGACTGGATCGACGACAACGGCGTCAGCCGCCAGGAAAGGAAAGCTCTACAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,135 total letters Query= IDP96598.102 Length=1053 Score E Sequences producing significant alignments: (Bits) Value 1_04B_B4_IDP96598.102_T7S.seq 1537 0.0 2_10B_B4_IDP96598.102_T7term.seq 1528 0.0 0_wt 1169 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L Query_1 1 TCTCGA-TCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAAC-TTT 88 1 23 ..........................C... 52 2 936 ......N.........-........................N........................................-...-... 850 2 869 ...G...........T.T.C.C.C...N.......A... 907 * E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E Query_1 89 AAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGA 178 1 53 ......................................------------------------------------------------.... 94 2 849 ..................N...................------------------------------------------------.... 808 N L Y F Q S N A G V T A E R T R L V F V E G Q R E A S L L L Query_1 179 ACCTGTACTTCCAATCCAATGCCGGAGTGACCGCGGAGCGCACCCGCCTGGTGTTCGTGGAGGGCCAGCGCGAGGCCTCGTTGCTGCTGG 268 1 95 .......................................................................................... 184 2 807 .......................................................................................... 718 0 1 ................................................................... 67 V N Q N P Y P V L V Q A W V D D G A L D G E P D T A L A P F Query_1 269 TCAACCAGAATCCCTACCCGGTGCTGGTGCAGGCGTGGGTCGATGACGGCGCCCTCGACGGCGAACCGGATACCGCGCTGGCTCCGTTCC 358 1 185 .......................................................................................... 274 2 717 .......................................................................................... 628 0 68 .......................................................................................... 157 L P L P P V F R L E P G R Q R S L R L L A T G Q A Q A R D R Query_1 359 TGCCGCTGCCGCCCGTATTCCGCCTGGAGCCGGGACGCCAGCGCAGCCTGCGCCTGCTGGCTACCGGCCAGGCCCAGGCACGGGATCGCG 448 1 275 .......................................................................................... 364 2 627 .......................................................................................... 538 0 158 .......................................................................................... 247 E S L Y W L N I Y E I P P Q A T A E A A G R P R L T V T L R Query_1 449 AATCGCTGTACTGGCTGAACATCTACGAGATCCCCCCGCAAGCCACCGCCGAGGCCGCTGGCCGGCCCCGGCTCACGGTCACCCTGCGAA 538 1 365 .......................................................................................... 454 2 537 .......................................................................................... 448 0 248 .......................................................................................... 337 T Q M K V L Y R P H G L H P H A E D A A A E L G F A L R D E Query_1 539 CCCAGATGAAGGTGCTGTACCGCCCGCACGGCCTGCATCCGCACGCCGAGGACGCGGCCGCCGAACTGGGCTTCGCGCTGCGCGACGAGA 628 1 455 .......................................................................................... 544 2 447 .......................................................................................... 358 0 338 .......................................................................................... 427 T L R V D N P T P Y F V S L A G L A L E I G E T R L D L P G Query_1 629 CCCTGCGGGTGGACAACCCGACGCCGTACTTCGTGTCCCTGGCGGGGCTGGCGCTGGAGATAGGGGAGACGCGCCTGGATCTGCCCGGAA 718 1 545 .......................................................................................... 634 2 357 .......................................................................................... 268 0 428 .......................................................................................... 517 T L L A P R S Q R R L G L P R R V D G G T R G E V A F D W I Query_1 719 CGCTGCTGGCTCCGCGCTCGCAACGACGCCTCGGCCTGCCGCGGCGGGTGGACGGCGGGACGCGCGGCGAGGTGGCGTTCGACTGGATCG 808 1 635 .......................................................................................... 724 2 267 .......................................................................................... 178 0 518 .......................................................................................... 607 D D N G V S R Q E R K A L Q * H W K W I T D P N S S S V D K Query_1 809 ACGACAACGGCGTCAGCCGCCAGGAAAGG-AAAGCTCTACAGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAG 897 1 725 ............N................A..............N............................................. 814 2 177 .............................-............................................................ 89 0 608 .............................-............ 648 L A A A L E H H H H H H * D P A A N K A R K E A E L A A A Query_1 898 CTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAG-CCCGAAAGGAAGCTGAG-TTGGCTGCTGCCA 985 1 815 ......NN..................................................-....-............T............. 902 2 88 ..........................................................C........... 19 T A E Q * L A * P L G A S K R V L R G F L L K Query_1 986 CCGCTGAGCAATAACTAGCATAA-CCCCTTGGGG-CCTCTAAACGGGTCTTGAGGGGTTTTTTG-CTGAAA 1053 1 903 ..........T............C..........T...........TCN...............T...... 973 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3201219 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 3,135 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96599.102 (IDP96599.30-230.P(230)..pMCSG53)
GATRVIYPSAAKQTSLAISNSDTQERYLVNSWIENSAGQKEKTFIVTPPLFVSEPKSENTLRIIYAGQPLPADRESLFWMNVKAIPSVDKSHIEGKNVLQLAILSRIKLFVRPANLPQTPEDAPTLLKFSRVGNHLKITNPSAYYLTLVNISVGAKKIDNVMIAPKSDMQIPLPTGAQGSVTFQTVNDYGALTSSTTASLG
GGCGCCACGCGAGTTATTTATCCCTCGGCGGCGAAGCAGACTTCTCTGGCAATCAGTAATAGCGATACTCAAGAACGTTACCTCGTCAATTCATGGATCGAAAATAGCGCCGGGCAGAAAGAAAAAACGTTTATTGTTACGCCGCCTTTATTCGTCAGCGAACCCAAAAGCGAAAACACGCTGCGTATTATCTACGCCGGGCAACCGCTACCCGCGGATCGGGAGTCGTTATTCTGGATGAACGTGAAAGCCATCCCGTCGGTCGATAAAAGTCATATTGAAGGAAAAAACGTTCTGCAACTGGCGATTCTGTCGCGCATCAAACTGTTCGTGCGTCCGGCGAATTTGCCGCAGACGCCGGAAGACGCGCCGACCTTGCTGAAATTTTCCCGTGTCGGCAACCATCTCAAGATAACCAACCCATCTGCTTATTACCTCACGCTGGTCAATATCAGCGTGGGCGCGAAAAAGATTGATAACGTGATGATCGCGCCAAAAAGCGACATGCAAATTCCCTTACCGACTGGCGCGCAGGGCAGCGTGACATTTCAGACCGTCAATGATTACGGCGCATTGACGTCGTCGACAACGGCCAGTCTCGGT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,082 total letters Query= IDP96599.102 Length=1008 Score E Sequences producing significant alignments: (Bits) Value 1_05H_H5_IDP96599.102_T7S.seq 1516 0.0 2_11H_H5_IDP96599.102_T7term.seq 1474 0.0 0_wt 1088 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAA 90 1 19 ..N..........-................... 50 2 889 .......................................................................................... 800 2 822 ...G...........T.T.C.C.C...........A... 860 E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E N Query_1 91 GAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAAC 180 1 51 ....................................------------------------------------------------...... 92 2 799 ....................................------------------------------------------------...... 758 L Y F Q S N A G A T R V I Y P S A A K Q T S L A I S N S D T Query_1 181 CTGTACTTCCAATCCAATGCCGGCGCCACGCGAGTTATTTATCCCTCGGCGGCGAAGCAGACTTCTCTGGCAATCAGTAATAGCGATACT 270 1 93 .......................................................................................... 182 2 757 .......................................................................................... 668 0 1 ..................................................................... 69 Q E R Y L V N S W I E N S A G Q K E K T F I V T P P L F V S Query_1 271 CAAGAACGTTACCTCGTCAATTCATGGATCGAAAATAGCGCCGGGCAGAAAGAAAAAACGTTTATTGTTACGCCGCCTTTATTCGTCAGC 360 1 183 .......................................................................................... 272 1 393 ...G........... 407 2 667 .......................................................................................... 578 2 457 ...G........... 443 0 70 .......................................................................................... 159 0 280 ...G........... 294 E P K S E N T L R I I Y A G Q P L P A D R E S L F W M N V K Query_1 361 GAACCCAAAAGCGAAAACACGCTGCGTATTATCTACGCCGGGCAACCGCTACCCGCGGATCGGGAGTCGTTATTCTGGATGAACGTGAAA 450 1 273 .......................................................................................... 362 2 577 .......................................................................................... 488 0 160 .......................................................................................... 249 A I P S V D K S H I E G K N V L Q L A I L S R I K L F V R P Query_1 451 GCCATCCCGTCGGTCGATAAAAGTCATATTGAAGGAAAAAACGTTCTGCAACTGGCGATTCTGTCGCGCATCAAACTGTTCGTGCGTCCG 540 1 363 .......................................................................................... 452 1 230 ...A........... 244 2 487 .......................................................................................... 398 2 620 ...A........... 606 2 917 ........ 910 0 250 .......................................................................................... 339 0 117 ...A........... 131 A N L P Q T P E D A P T L L K F S R V G N H L K I T N P S A Query_1 541 GCGAATTTGCCGCAGACGCCGGAAGACGCGCCGACCTTGCTGAAATTTTCCCGTGTCGGCAACCATCTCAAGATAACCAACCCATCTGCT 630 1 453 .......................................................................................... 542 2 397 .......................................................................................... 308 2 909 ... 907 0 340 .......................................................................................... 429 Y Y L T L V N I S V G A K K I D N V M I A P K S D M Q I P L Query_1 631 TATTACCTCACGCTGGTCAATATCAGCGTGGGCGCGAAAAAGATTGATAACGTGATGATCGCGCCAAAAAGCGACATGCAAATTCCCTTA 720 1 543 .......................................................................................... 632 2 307 .......................................................................................... 218 0 430 .......................................................................................... 519 P T G A Q G S V T F Q T V N D Y G A L T S S T T A S L G * H Query_1 721 CCGACTGGCGCGCAGGGCAGCGTGACATTTCAGACCGTCAATGATTACGGCGCATTGACGTCGTCGACAACGGCCAGTCTCGGTTAACAT 810 1 633 .......................................................................................... 722 2 217 .......................................................................................... 128 0 520 .................................................................................... 603 W K W I T D P N S S S V D K L A A A L E H H H H H H * D P A Query_1 811 TGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCT 900 1 723 .......................................................................................... 812 2 127 .......................................................................................... 38 A N K A R K E A E L A A A T A E Q * L A * P L G A S K R V L Query_1 901 GCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTG 990 1 813 .....................................................................-.................... 901 2 37 .............NN..A.... 16 R G F L L Query_1 991 AGGGGTTTTTTGCTGAA 1007 1 902 ................. 918 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3015741 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 3,082 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96600.102 (IDP96600.30-232.P(232)..pMCSG53)
STRVIYDGSRKEASLTVNNKSTTDEFLIQSWIDDANGNKKTPFIITPPLFKLSPTKNNVLRIVNTTNTLPQDRESVYWINVKAIPAKSEDAEAKNVLQIAVRTRLKLFYRPAGLKGNSMDGWNKLQFTSAGANQIKVENPSAFNLTFNKFYANGRDIEKTGMVPAKGSLNIELPAGTGKVSEVKYNIINDFGTAGDMLTQRVN
AGCACGCGTGTTATTTATGACGGTAGTAGAAAGGAAGCTTCTCTTACGGTAAATAATAAAAGCACCACGGATGAATTTCTCATTCAGTCATGGATTGATGATGCTAACGGTAATAAAAAGACGCCCTTTATCATCACTCCACCGTTATTTAAATTAAGCCCGACTAAAAATAACGTTTTACGTATTGTTAATACGACGAACACGTTACCGCAGGATCGCGAGTCCGTTTATTGGATTAACGTAAAAGCTATTCCTGCCAAAAGTGAAGACGCGGAAGCTAAAAACGTACTGCAGATCGCCGTACGTACCCGCTTAAAACTGTTCTATCGCCCGGCGGGCCTGAAAGGCAATAGCATGGACGGCTGGAACAAACTGCAGTTCACCAGCGCAGGGGCTAACCAGATCAAAGTGGAAAACCCATCTGCCTTTAACCTGACGTTTAATAAATTTTATGCCAACGGCCGTGATATTGAAAAAACGGGAATGGTTCCGGCAAAAGGCTCATTGAATATTGAACTGCCAGCCGGCACCGGCAAGGTAAGCGAAGTTAAATACAACATTATTAATGACTTTGGCACTGCTGGCGACATGTTGACACAGCGCGTTAAC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,411 total letters Query= IDP96600.102 Length=1014 Score E Sequences producing significant alignments: (Bits) Value 2_12A_A6_IDP96600.102_T7term.seq 1443 0.0 0_wt 1099 0.0 1_06A_A6_IDP96600.102_T7S.seq 924 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCT-CTAGAAATAATTTTGTTT-AACTTT 88 2 901 ................................................................-.................N-.-.... 815 2 834 ...G...........T.T.C.C.C...........-A... 872 1 22 .....N.......A.T........T...... 52 * E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E Query_1 89 AAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGA 178 2 814 ......................................------------------------------------------------.... 773 1 53 ......................................------------------------------------------------.... 94 N L Y F Q S N A S T R V I Y D G S R K E A S L T V N N K S T Query_1 179 ACCTGTACTTCCAATCCAATGCCAGCACGCGTGTTATTTATGACGGTAGTAGAAAGGAAGCTTCTCTTACGGTAAATAATAAAAGCACCA 268 2 772 .......................................................................................... 683 0 1 ................................................................... 67 1 95 .......................................................................................... 184 T D E F L I Q S W I D D A N G N K K T P F I I T P P L F K L Query_1 269 CGGATGAATTTCTCATTCAGTCATGGATTGATGATGCTAACGGTAATAAAAAGACGCCCTTTATCATCACTCCACCGTTATTTAAATTAA 358 2 682 .......................................................................................... 593 0 68 .......................................................................................... 157 1 185 .......................................................................................... 274 S P T K N N V L R I V N T T N T L P Q D R E S V Y W I N V K Query_1 359 GCCCGACTAAAAATAACGTTTTACGTATTGTTAATACGACGAACACGTTACCGCAGGATCGCGAGTCCGTTTATTGGATTAACGTAAAAG 448 2 592 .......................................................................................... 503 0 158 .......................................................................................... 247 1 275 .....................................N..N.....N........................................... 364 A I P A K S E D A E A K N V L Q I A V R T R L K L F Y R P A Query_1 449 CTATTCCTGCCAAAAGTGAAGACGCGGAAGCTAAAAACGTACTGCAGATCGCCGTACGTACCCGCTTAAAACTGTTCTATCGCCCGGCGG 538 2 502 .......................................................................................... 413 0 248 .......................................................................................... 337 1 365 .......................................................................................... 454 G L K G N S M D G W N K L Q F T S A G A N Q I K V E N P S A Query_1 539 GCCTGAAAGGCAATAGCATGGACGGCTGGAACAAACTGCAGTTCACCAGCGCAGGGGCTAACCAGATCAAAGTGGAAAACCCATCTGCCT 628 2 412 .......................................................................................... 323 0 338 .......................................................................................... 427 1 455 ...............................................................N.......................... 544 F N L T F N K F Y A N G R D I E K T G M V P A K G S L N I E Query_1 629 TTAACCTGACGTTTAATAAATTTTATGCCAACGGCCGTGATATTGAAAAAACGGGAATGGTTCCGGCAAAAGGCTCATTGAATATTGAAC 718 2 322 .......................................................................................... 233 0 428 .......................................................................................... 517 1 545 .............................................................. 606 L P A G T G K V S E V K Y N I I N D F G T A G D M L T Q R V Query_1 719 TGCCAGCCGGCACCGGCAAGGTAAGCGAAGTTAAATACAACATTATTAATGACTTTGGCACTGCTGGCGACATGTTGACACAGCGCGTTA 808 2 232 .......................................................................................... 143 0 518 .......................................................................................... 607 N * H W K W I T D P N S S S V D K L A A A L E H H H H H H Query_1 809 ACTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcacc-accacc-accaccacTG 896 2 142 ........................................................................A......A.......... 53 0 608 .. 609 * D P A A N K A R K E A Query_1 897 AGATCCGGCTGCTAA--CAAAGCCCGAAAGGAAGCT 930 2 52 ...............ACA....... 28 2 721 ........... 711 0 29 ........... 39 1 146 ........... 156 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2363634 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 2,411 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96601.102 (IDP96601.22-477.P(477)..pMCSG53)
DDLPSLGDASSSIVSPEQEFQLGRAWLSMLRNQVDAISDPQLKDFVESSVYRLAETSDLQDHRLAFILIRDAQINAFAAPGGVIGVNGGLLLNAQTEGEYAAVLAHELGHLIQRHFARGIEAQQRMQLPVMAAMLAGIVAAAAGAGDAGIAAIAGTQAAAIQNQLRFSRQNEQEADRVGVTNMVRAGYDPRSMPNMFERLARQYRYDGKPPEFLLTHPVTESRIADTRNRAEQYPQGGKEDSLRYQQMRARTQLLFEETPGMAGKRFRAMLNDNPKLDAARYGLAISQTRIGQLNDARDNLRQLLAKAPNDVAYNLAQVELDITANRLPDADQRLKKMLAQFPSSYPLKQVRADLMLKMGKPQESEKILDDLSKARPLDPDVWNRLAEVRSLTNNAIGVHQARAEYLSLTGDYQAAIEQLDFAKRRAGGNFQLAARLDARQQEIRQQEKMVKEMFR
GACGATCTGCCCTCCCTGGGCGACGCATCTTCCTCGATCGTTTCGCCGGAGCAGGAGTTCCAGCTGGGCCGCGCCTGGCTGAGCATGCTGCGCAACCAGGTCGATGCGATCTCCGACCCGCAGCTCAAGGACTTCGTCGAAAGCAGCGTCTACCGGCTGGCCGAAACCAGCGACCTGCAGGATCACCGGCTGGCCTTCATCCTCATCCGCGACGCACAGATCAACGCCTTCGCCGCGCCCGGCGGCGTGATCGGGGTCAACGGCGGGCTACTGCTCAACGCCCAGACCGAAGGCGAGTATGCCGCGGTGCTGGCCCACGAACTCGGCCACCTGATCCAGCGCCACTTCGCCCGCGGCATCGAGGCACAGCAGCGCATGCAACTGCCGGTGATGGCAGCGATGCTCGCCGGCATCGTCGCCGCGGCCGCCGGCGCAGGGGATGCGGGTATCGCGGCGATCGCCGGGACCCAGGCGGCGGCGATCCAGAACCAGTTGCGCTTTTCCCGGCAGAACGAGCAGGAAGCCGACCGCGTCGGCGTCACCAACATGGTTCGCGCCGGTTACGATCCGCGCTCGATGCCGAACATGTTCGAGCGCCTGGCGCGGCAGTACCGCTACGACGGCAAGCCGCCGGAGTTCCTCCTCACCCACCCGGTCACCGAATCGCGTATCGCCGACACCCGCAACCGCGCCGAACAGTATCCCCAGGGCGGCAAGGAAGACAGCTTGCGCTACCAGCAGATGCGCGCGCGCACCCAGTTGCTCTTCGAGGAAACCCCGGGGATGGCCGGCAAGCGCTTCCGCGCCATGCTCAACGACAATCCCAAGCTGGATGCCGCGCGCTACGGACTGGCCATCTCGCAGACGCGCATCGGCCAGCTCAACGATGCCCGTGACAACCTCAGGCAATTGCTGGCGAAGGCACCCAACGACGTGGCCTACAACCTGGCCCAGGTCGAGCTGGACATCACCGCCAACCGCCTGCCGGATGCCGACCAGCGGTTGAAGAAGATGCTCGCCCAGTTCCCCAGCAGCTACCCGCTGAAACAGGTGCGCGCCGACCTGATGCTGAAGATGGGCAAGCCGCAGGAATCGGAGAAGATCCTCGACGACCTGAGCAAGGCGCGGCCGCTGGACCCGGATGTCTGGAACCGCCTGGCCGAGGTGCGCAGCCTGACCAACAACGCCATCGGCGTGCACCAGGCCCGGGCCGAGTACCTGTCTCTGACCGGCGACTACCAGGCCGCCATCGAGCAGCTCGATTTCGCCAAGCGCCGCGCTGGCGGCAACTTCCAACTGGCTGCGCGACTCGACGCTCGCCAGCAGGAAATTCGCCAGCAGGAAAAGATGGTCAAGGAAATGTTCCGC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,812 total letters Query= IDP96601.102 Length=1773 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2468 0.0 2_10C_C4_IDP96601.102_T7term.seq 1876 0.0 1_04C_C4_IDP96601.102_T7S.seq 1696 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 60 CCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTC 149 1 23 ...T...............................................................----------------------- 89 S H P Q F E K G T E N L Y F Q S N A D D L P S L G D A S S S Query_1 150 TCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGACGATCTGCCCTCCCTGGGCGACGCATCTTCCTCGAT 239 0 1 ...................................... 38 1 90 -------------------------................................................................. 154 I V S P E Q E F Q L G R A W L S M L R N Q V D A I S D P Q L Query_1 240 CGTTTCGCCGGAGCAGGAGTTCCAGCTGGGCCGCGCCTGGCTGAGCATGCTGCGCAACCAGGTCGATGCGATCTCCGACCCGCAGCTCAA 329 0 39 .......................................................................................... 128 1 155 .......................................................................................... 244 K D F V E S S V Y R L A E T S D L Q D H R L A F I L I R D A Query_1 330 GGACTTCGTCGAAAGCAGCGTCTACCGGCTGGCCGAAACCAGCGACCTGCAGGATCACCGGCTGGCCTTCATCCTCATCCGCGACGCACA 419 0 129 .......................................................................................... 218 1 245 .......................................................................................... 334 Q I N A F A A P G G V I G V N G G L L L N A Q T E G E Y A A Query_1 420 GATCAACGCCTTCGCCGCGCCCGGCGGCGTGATCGGGGTCAACGGCGGGCTACTGCTCAACGCCCAGACCGAAGGCGAGTATGCCGCGGT 509 0 219 .......................................................................................... 308 1 335 .......................................................................................... 424 V L A H E L G H L I Q R H F A R G I E A Q Q R M Q L P V M A Query_1 510 GCTGGCCCACGAACTCGGCCACCTGATCCAGCGCCACTTCGCCCGCGGCATCGAGGCACAGCAGCGCATGC-AACTGCCGGTG-ATGGCA 597 0 309 .......................................................................-...........-...... 396 2 1139 ......N......N...AT.N.... 1115 1 425 .......................................................................-...........-...... 512 A M L A G I V A A A A G A G D A G I A A I A G T Q A A A Query_1 598 GCGAT-GCTCGCCGGCATCGT-CGCCGCGGCCGCCGGC-GCAGGGGATGCGGGTATCGCGGCGATCGCCGGGACCCA-GGCGGCGGCGAT 683 0 397 .....-...............-................-......................................-............ 482 0 466 ..C............C.. 449 2 1114 ..N..A.N......N......T....NNC........NN....A.................................A............ 1025 2 1042 ..C............C.. 1059 1 513 .....-...............-................-......................................-............ 598 1 582 ..C............C.. 565 I Q N Q L R F S R Q N E Q E A D R V G V T N M V R A G Y D P Query_1 684 CCAGAACCAGTTGCGCTTTTCCCGGCAGAACGAGCAGGAAGCCGACC-GCGTCGGCGTC-ACCAACATGGTTCGCGCCGGTTACGATCCG 771 0 483 ...............................................-...........-.............................. 570 2 1024 ......................N..........N.............C...........N....................N......... 935 1 599 ...............................................-...........-.............................. 686 R S M P N M F E R L A R Q Y R Y D G K P P E F L L T H P V Query_1 772 CGCTCGATGCCGAACATGTTCGAGCGCCTGGCGCGG-CAGTACCGCTACGACGGCAAGCCGCCGGA-GTTCCTCCTCACCCACCCGGTCA 859 0 571 ....................................-.............................-....................... 658 0 592 ............ 581 2 934 .......N............................G.............................A....................... 845 2 913 ............ 924 1 687 ....................................-.............................-....................... 774 1 708 ............ 697 T E S R I A D T R N R A E Q Y P Q G G K E D S L R Y Q Q M Query_1 860 -CCGAATCGCGTATCGCCGACACCCGCAACCGCG-CCGAACAGTATCCCCAGGGCGGCAAGGAAGACAGCTTGCGCTA-CCAGCAGATGC 946 0 659 -.................................-...........................................-........... 745 0 754 ... 752 2 844 A.................................-...........................................-........... 757 2 748 ... 750 1 775 -.................................C...........................................C........... 863 1 872 ... 870 R A R T Q L L F E E T P G M A G K R F R A M L N D N P K L Query_1 947 GCGCGCGCACCCAGTTGCTCTTCGAGGAAACCCCGGGGATGGC-CGGCAAGCGC-TTCCGCGCCATGCTCAACGACAATCCCAAGCTGGA 1034 0 746 ...........................................-..........-................................... 833 0 751 ......... 743 2 756 ...........................................-..........-................................... 669 2 751 ......... 759 1 864 ..............................-............-...-......T..............N............N....... 950 1 926 .....G...A......-.....-..... 901 1 869 ......... 861 D A A R Y G L A I S Q T R I G Q L N D A R D N L R Q L L Query_1 1035 TG-CCGCGCGCTACGGACTGGCCATC-TCGCAGACGCGCATCGGCCAGCTCAACGA-TGCCC-GTGACAACCTCAGGC-AATTGCT-GGC 1118 0 834 ..-.......................-.............................-.....-...............-.......-... 917 2 668 ..-.......................-.............................-.....-...............-.......-... 585 1 951 ..C.......................T...N.............T...........N.....G...............A.....N.G... 1040 A K A P N D V A Y N L A Q V E L D I T A N R L P D A D Query_1 1119 GAAGGCACCC-AACG-ACGTGGCCT-ACAACCTGGCC-CA-GGTCGAG-CTGG-ACATCACCGCCAACC-GCCT-GCCGGAT-GCCGACC 1198 0 918 ..........-....-.........-...........-..-.......-....-...............-....-.......-....... 997 2 584 ..........-....-.........-...........-..-.......-....-...............-....-.......-....... 505 1 1041 N....N....A....A.........N...........T..N.......N....A..N........C...NN...G......NG.....N. 1130 Q R L K K M L A Q F P S S Y P L K Q V R A D L M L K M G K Query_1 1199 -AGCGGTTGAAG--AAGATGCTCGCCCAGTTCCCCAGCAGCTACCCGCTGAAACAGGTGCGCGCCGACCTGATGCTGAAGATGGGCAAGC 1285 0 998 -...........--............................................................................ 1084 2 504 -...........--............................................................................ 418 1 1131 TNT....GN...AA......... 1153 P Q E S E K I L D D L S K A R P L D P D V W N R L A E V R S Query_1 1286 CGCAGGAATCGGAGAAGATCCTCGACGACCTGAGCAAGGCGCGGCCGCTGGACCCGGATGTCTGGAACCGCCTGGCCGAGGTGCGCAGCC 1375 0 1085 .......................................................................................... 1174 2 417 .......................................................................................... 328 L T N N A I G V H Q A R A E Y L S L T G D Y Q A A I E Q L D Query_1 1376 TGACCAACAACGCCATCGGCGTGCACCAGGCCCGGGCCGAGTACCTGTCTCTGACCGGCGACTACCAGGCCGCCATCGAGCAGCTCGATT 1465 0 1175 .......................................................................................... 1264 2 327 .......................................................................................... 238 F A K R R A G G N F Q L A A R L D A R Q Q E I R Q Q E K M V Query_1 1466 TCGCCAAGCGCCGCGCTGGCGGCAACTTCCAACTGGCTGCGCGACTCGACGCTCGCCAGCAGGAAATTCGCCAGCAGGAAAAGATGGTCA 1555 0 1265 .......................................................................................... 1354 2 237 .......................................................................................... 148 K E M F R * H W K W I T D P N S S S V D K L A A A L E H H H Query_1 1556 AGGAAATGTTCCGCTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccacc 1645 0 1355 .............. 1368 2 147 .......................................................................................... 58 H H H * D P A A N K A R K E Query_1 1646 accaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAG 1687 2 57 .....................................A.... 16 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 6613348 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 3,812 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96606.102 (IDP96606.28-487.P(487)..pMCSG53)
DPADTLPDMGTSAGSTLSIGQEMQMGDFYVRQLRGSAPLINDPLLVQYINALGMRLVSHADSVKTPFHFFLINNDEINAFAFFGGNVVLHSALFRYADNESQLASVMAHEISHVTQRHLARAMEDQKRSAPLTWVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRAGFDPQAMPSFLEKLLDQARYSTRPPEILLTHPLPESRLADARNRANQMRPVVVQSSADFYFAKARALGMYNSGRNQLTSDLLDQWSKGNVRQQHAAQYGRALQAMEASKYDEARKTLQPLLSAEPNNAWYLDLATDIDLGQKRANDAINRLKNARDLRVNPVLQLNLANAYLQGGQPKAAETILNRYTFSHKDDGNGWDLLAQAEAALNNRDQELAARAESYALAGRLDQAISLLSSASAQAKLGSQQQARYDARIDQLRQLQERFKPYTKM
GACCCTGCCGACACGCTGCCCGATATGGGAACCTCGGCAGGAAGCACGCTTTCTATCGGACAAGAGATGCAAATGGGCGACTTTTATGTGCGCCAGCTACGCGGCAGCGCGCCGTTAATCAACGATCCGCTGCTGGTGCAATACATTAACGCGCTGGGTATGCGTCTGGTCTCGCACGCCGACTCCGTCAAAACGCCCTTCCATTTTTTCTTGATCAATAATGACGAAATCAACGCCTTCGCGTTCTTTGGCGGCAATGTGGTGCTGCACTCGGCGCTTTTTCGCTACGCGGATAACGAAAGCCAGCTAGCTTCAGTCATGGCGCATGAAATCTCCCACGTGACGCAGCGCCACCTGGCGCGCGCGATGGAAGATCAAAAGCGCAGCGCGCCGCTTACCTGGGTGGGCGCGCTGGGTTCCATTTTGCTGGCCATGGCCAGCCCACAGGCCGGTATGGCGGCGCTAACCGGTACTCTGGCGGGAACGCGCCAGGGAATGATAAGTTTCACCCAGCAAAATGAGCAAGAAGCCGACCGTATTGGTATTCAGGTACTGCAACGCGCCGGATTTGACCCACAGGCGATGCCCTCTTTCCTCGAAAAACTGCTCGACCAGGCGCGTTACTCCACGCGCCCGCCTGAAATTTTGCTCACTCACCCCTTACCGGAAAGCCGCCTTGCGGATGCCCGCAACCGTGCCAACCAGATGCGCCCGGTCGTGGTGCAATCTTCCGCCGACTTTTATTTCGCCAAAGCGCGCGCCCTGGGAATGTACAATTCCGGACGTAACCAGCTTACCAGCGACCTGCTGGATCAGTGGTCTAAAGGCAACGTGCGTCAGCAACATGCGGCGCAATATGGCCGGGCGTTGCAGGCGATGGAAGCGAGCAAGTACGATGAAGCGCGCAAAACGTTGCAGCCGCTGTTAAGCGCGGAACCGAACAATGCCTGGTATCTTGACCTCGCCACCGATATTGACCTGGGGCAGAAAAGAGCCAACGACGCGATTAATCGCCTGAAAAATGCCCGCGATCTGCGCGTTAATCCTGTGCTGCAGTTAAACCTCGCCAATGCGTACCTCCAGGGAGGCCAGCCGAAAGCGGCGGAAACCATTCTGAATCGCTACACCTTTAGCCATAAAGATGACGGTAACGGCTGGGATCTGCTTGCTCAGGCCGAAGCCGCGCTGAACAACCGCGATCAGGAGCTGGCGGCGCGCGCTGAAAGTTATGCGCTGGCGGGACGACTGGATCAGGCAATTTCACTGCTCAGTAGCGCCAGCGCCCAGGCAAAACTGGGTAGTCAGCAACAGGCGCGTTACGATGCGCGTATCGACCAGTTGCGCCAGTTACAGGAACGCTTCAAGCCATACACGAAAATG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,848 total letters Query= IDP96606.102 Length=1785 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2489 0.0 2_12B_B6_IDP96606.102_T7term.seq 1911 0.0 1_06B_B6_IDP96606.102_T7S.seq 1755 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 56 AATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGT 145 1 16 ...........N...........................................................------------------- 86 W S H P Q F E K G T E N L Y F Q S N A D P A D T L P D M G T Query_1 146 GGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGACCCTGCCGACACGCTGCCCGATATGGGAACCT 235 0 1 .................................. 34 1 87 -----------------------------............................................................. 147 S A G S T L S I G Q E M Q M G D F Y V R Q L R G S A P L I N Query_1 236 CGGCAGGAAGCACGCTTTCTATCGGACAAGAGATGCAAATGGGCGACTTTTATGTGCGCCAGCTACGCGGCAGCGCGCCGTTAATCAACG 325 0 35 .......................................................................................... 124 0 383 ........... 393 1 148 .......................................................................................... 237 1 496 ........... 506 D P L L V Q Y I N A L G M R L V S H A D S V K T P F H F F L Query_1 326 ATCCGCTGCTGGTGCAATACATTAACGCGCTGGGTATGCGTCTGGTCTCGCACGCCGACTCCGTCAAAACGCCCTTCCATTTTTTCTTGA 415 0 125 .......................................................................................... 214 1 238 .......................................................................................... 327 I N N D E I N A F A F F G G N V V L H S A L F R Y A D N E S Query_1 416 TCAATAATGACGAAATCAACGCCTTCGCGTTCTTTGGCGGCAATGTGGTGCTGCACTCGGCGCTTTTTCGCTACGCGGATAACGAAAGCC 505 0 215 .......................................................................................... 304 1 328 .......................................................................................... 417 Q L A S V M A H E I S H V T Q R H L A R A M E D Q K R S A Query_1 506 AGCTAGCTTCAGTCATGGCGCATGAAATCTCCCACGTGACGCAGCGCCACCTGGCGCGCGCGATGGAAGATCAAAAGCGCA--GCGCGCC 593 0 305 .................................................................................--....... 392 0 104 ...--....... 113 2 1155 .............NN...AGNN..... 1129 1 418 .................................................................................--....... 505 1 217 ...--....... 226 P L T W V G A L G S I L L A M A S P Q A G M A A L T G T L A Query_1 594 GCTTACCTGGGTGGGCGCGCTGGGTTCCATTTTGCTGGCCATGGCCAGCCCACAGGCCGGTAT-GGCGGCGCTAACC-GGTACTCTGGCG 681 0 393 ...............................................................-.............-............ 480 0 441 ................ 426 0 114 . 114 0 520 ........... 510 2 1128 ....N.T...................N.CA.......N.....A...N.......N.......T......N......C......N..... 1039 1 506 ...............................................................-.............-............ 593 1 554 ................ 539 1 227 . 227 1 633 ........... 623 G T R Q G M I S F T Q Q N E Q E A D R I G I Q V L Q R A G Query_1 682 GGAACGCGCCAGGGAA-TGATAAGTTTCACCCAGCAAAATGAGCAAGAAGCCGACCGTATTGGTATTCAGGTACTGCAACGCGCCGGATT 770 0 481 ................-......................................................................... 569 0 430 ........... 420 2 1038 ...CG...........N.....................-................................................... 950 1 594 ................-......................................................................... 682 1 543 ........... 533 F D P Q A M P S F L E K L L D Q A R Y S T R P P E I L L T H Query_1 771 TGACCCACAGGCGATGCCCTCTTTCCTCGAAAAACTGCTCGACCAGGCGCGTTACTCCACGCGCCCGCCTGAAA--TTTTGCTCACTCAC 858 0 570 ..........................................................................--.............. 657 0 1309 ............ 1320 2 949 ..........................................................................--.............. 862 2 209 ............ 198 1 683 ..........................................................................AT.............. 772 P L P E S R L A D A R N R A N Q M R P V V V Q S S A D F Y Query_1 859 CCCTTACCGGAAAGCCG-CCTTGCGGATGCCCGCAACCGTGCCAACCAGATGCGCCCGGTCGTGGTGCAATCTTCCGCCGACTTTTATTT 947 0 658 .................-........................................................................ 746 2 861 .................-........................................................................ 773 1 773 ..........-......C........................................................................ 861 F A K A R A L G M Y N S G R N Q L T S D L L D Q W S K G N V Query_1 948 CGCCAAAGCGCGCGCCCTGGGAATGTACAATTCCGGACGTAACCAGCTTACCAGCGACCTGCTGG-ATCAGTGGTCTAAAGGCAACGTGC 1036 0 747 .................................................................-........................ 835 2 772 .................................................................G........................ 683 1 862 .........................................................T.......-........................ 950 R Q Q H A A Q Y G R A L Q A M E A S K Y D E A R K T L Q P Query_1 1037 GTCAGCAACATGCGGCGCAATAT-GGCCGGGC-GTTGCAGGCGATGGAAGCGAGCAAGTACGATGAAGCGCGCAAAACG-TTGCAGCCGC 1123 0 836 .......................-........-..............................................-.......... 922 2 682 .......................-........-..............................................-.......... 596 1 951 .......................G........NN................N.........................N..T.....NT... 1040 L L S A E P N N A W Y L D L A T D I D L G Q K R A N Query_1 1124 -TGTTAAGCGCGG-AACCGAAC-AATGCCTGGTAT-CTTGACCT-CGCCA-CCGA-TATTGACCT-GGGGCAGAAAAGAG--CCAAC-GA 1202 0 923 -............-........-............-........-.....-....-.........-..............--.....-.. 1001 2 595 -............-........-............-........-.....-....-.........-..............--.....-.. 517 1 1041 T....T.......A.....G..A............C..G.....N.....C....T.........G.....NN....NNNNC.....NN. 1130 D A I N R L K N A R D L R V N P V L Q L N L A N A Y Query_1 1203 CGC-GATTAATCG-CCTGAAAAATGCCCGCG-ATCTGCGCGTTAAT--CCT-GTGC-TGCA-GTTAAACCTC---GCCAATGC-GTACCT 1280 0 1002 ...-.........-.................-..............--...-....-....-..........---........-...... 1079 0 1014 ..-.........-. 1003 2 516 ...-.........-.................-..............--...-....-....-..........---........-...... 439 2 504 ..-.........-. 515 1 1131 ...G....N...NC..N......NN......A.NNG.NN...N...TC...G....T....G..N.......TCT...NNNN.G.N.... 1220 L Q G G Q P K A A E T I L N R Y T F S H K D D G N G W D L L Query_1 1281 CCAGGGAGGCCA-GCCGAAAGCGGCGGAAACCATTCTGAATCGCTACACCTTTAGCCATAAAGATGACGGTAACGGCTGGGATCTGCTTG 1369 0 1080 ............-............................................................................. 1168 2 438 ............-............................................................................. 350 1 1221 T.NN..NN.N..G....G......N..N..N.... 1255 A Q A E A A L N N R D Q E L A A R A E S Y A L A G R L D Q A Query_1 1370 CTCAGGCCGAAGCCGCGCTGAACAACCGCGATCAGGAGCTGGCGGCGCGCGCTGAAAGTTATGCGCTGGCGGGACGACTGGATCAGGCAA 1459 0 1169 .......................................................................................... 1258 2 349 .......................................................................................... 260 I S L L S S A S A Q A K L G S Q Q Q A R Y D A R I D Q L R Q Query_1 1460 TTTCACTGCTCAGTAGCGCCAGCGCCCAGGCAAAACTGGGTAGTCAGCAACAGGCGCGTTACGATGCGCGTATCGACCAGTTGCGCCAGT 1549 0 1259 .......................................................................................... 1348 0 613 ............ 624 2 259 .......................................................................................... 170 2 906 ............ 895 1 726 ............ 737 L Q E R F K P Y T K M * H W K W I T D P N S S S V D K L A A Query_1 1550 TACAGGAACGCTTCAAGCCATACACGAAAATGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCG 1639 0 1349 ................................ 1380 2 169 .......................................................................................... 80 A L E H H H H H H * D P A A N K A R Query_1 1640 CACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAA--GCCCGAAA 1694 2 79 ...............................................AGC....... 23 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 6722200 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 3,848 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96668.102 (IDP96668.1-310.F..pMCSG53)
MRIVVALGGNALLRRGEPMTADNQRENVRIAAEQIAKVAPGNELVIAHGNGPQVGLLALQGAAYDKVSPYPLDVLGAETEGMIGYMIEQEMGNLLPFEVPFATILTQVEVDGKDPAFQNPTKPIGPVYSREEAERLAAEKGWSIAPDGDKFRRVVPSPRPKRIFEIRPVKWLLEKGTIVICAGGGGIPTMYDEAGKKLSGVEAVIDKDLCSSLLAQELVADILIIATDVDAAYVDWGKPTQKAIAQAHPDELERLGFAAGSMGPKVQAAIEFARATGKDAVIGSLADIVAITEGKAGTRVSTRKAGIEYR
ATGCGTATCGTCGTCGCATTGGGCGGTAATGCCCTGCTACGCCGTGGCGAACCCATGACCGCGGACAACCAGCGGGAAAACGTGCGGATCGCCGCCGAACAGATCGCCAAGGTGGCGCCCGGCAACGAGCTGGTGATCGCCCACGGCAACGGCCCCCAGGTCGGCCTGCTGGCCCTCCAGGGTGCCGCCTACGACAAGGTCTCGCCCTACCCGCTGGACGTCCTCGGCGCGGAAACCGAGGGCATGATCGGCTACATGATCGAACAGGAAATGGGCAACCTGCTGCCGTTCGAAGTGCCGTTCGCCACCATCCTCACCCAGGTCGAGGTGGACGGTAAGGATCCGGCGTTCCAGAACCCGACCAAGCCGATCGGCCCGGTCTACTCCAGGGAAGAAGCCGAGCGCCTGGCCGCCGAGAAGGGCTGGAGCATCGCCCCGGACGGCGACAAGTTCCGCCGCGTGGTGCCCAGCCCGCGGCCGAAGCGGATCTTCGAGATCCGCCCGGTGAAGTGGCTGCTGGAGAAAGGCACCATCGTCATCTGCGCTGGCGGCGGCGGTATCCCGACCATGTACGACGAGGCCGGGAAGAAGCTCAGCGGCGTCGAGGCGGTGATCGACAAGGACCTCTGCTCCTCGCTGCTGGCCCAGGAGCTGGTGGCCGACATCCTGATCATCGCCACCGACGTCGACGCGGCCTACGTCGACTGGGGCAAGCCGACCCAGAAGGCCATCGCCCAGGCCCACCCGGACGAACTGGAGCGCCTCGGCTTCGCCGCCGGCTCCATGGGTCCGAAGGTCCAGGCCGCCATCGAGTTCGCCCGCGCCACCGGCAAGGACGCGGTGATCGGTTCGCTCGCGGACATCGTCGCAATCACCGAGGGCAAGGCGGGAACACGGGTCAGCACGCGCAAGGCGGGCATCGAGTACCGC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,454 total letters Query= IDP96668.102 Length=1335 Score E Sequences producing significant alignments: (Bits) Value 1_03H_H3_IDP96668.102_T7S.seq 1867 0.0 2_09H_H3_IDP96668.102_T7term.seq 1811 0.0 0_wt 1678 0.0 V S G * Q F P S R N N F V * L * E G D I H M H H H H H H Query_1 42 TGTGAGCGGAT--AACAATTCCCC-TCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTC 128 1 16 .........C........-......................................................-- 87 2 1186 ..A........TN.N.N.......-NN-......N.A...NN.N................N.NN.......N.......--NN....... 1101 S S G V D L W S H P Q F E K G T E N L Y F Q S N A M R I V Query_1 129 TTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTT-CCAAT-CCAATGCCATGCGTAT-CGTCGT 215 1 88 ----------------------------------------------..............-.....-................-...... 128 2 1097 ..............T.....T............NNN.T...... 1054 0 1 ........-...... 14 V A L G G N A L L R R G E P M T A D N Q R E N V R I A A Query_1 216 CGCATT-GGGCGGTAATGCCCT-GCTACGCCGTGGCGAA--CCCATGACCGCGGACAACCA-GCGGG-AAAACGTGCGGATCGCCGCCGA 299 1 129 ......-...............-................--....................-.....-...................... 212 2 1053 ......T........N-.....N..............G.AC....................A.....G...................... 965 0 15 ......-...............-................--....................-.....-...................... 98 E Q I A K V A P G N E L V I A H G N G P Q V G L L A L Q G A Query_1 300 ACAGATCGCCAAGGTGGCGCCCGG-CAACGA-GCTGGTGATCGCCCACGGCAACGGCCCCCAGGTCGGCCTGCTGGCCCTCCAGGGTGCC 387 1 213 ........................-......-.......................................................... 300 2 964 ......................N.N......A.............-...................................-........ 877 0 99 ........................-......-.......................................................... 186 A Y D K V S P Y P L D V L G A E T E G M I G Y M I E Q E M Query_1 388 GCCTACGACAAGGTCTCGCCCTACCCGCTGGACGTCCTCGGCGCGGAAACCGAGGGCATGATCGGCT-ACATGATCGAACAGGAAATGGG 476 1 301 ...................................................................-...................... 389 2 876 ...................................................................N...................... 787 0 187 ...................................................................-...................... 275 G N L L P F E V P F A T I L T Q V E V D G K D P A F Q N P T Query_1 477 CAACCTGCTGCCGTTCGAAGTGCCGTTCGCCACCATCCTCACCCAGGTCGAGGTGGACGGTAAGGATCCGGCGTTCCAGAACCCGACCAA 566 1 390 .......................................................................................... 479 2 786 .......................................................................................... 697 0 276 .......................................................................................... 365 K P I G P V Y S R E E A E R L A A E K G W S I A P D G D K F Query_1 567 GCCGATCGGCCCGGTCTACTCCAGGGAAGAAGCCGAGCGCCTGGCCGCCGAGAAGGGCTGGAGCATCGCCCCGGACGGCGACAAGTTCCG 656 1 480 ...................................................................A...................... 569 2 696 ...................................................................A...................... 607 0 366 .......................................................................................... 455 R R V V P S P R P K R I F E I R P V K W L L E K G T I V I C Query_1 657 CCGCGTGGTGCCCAGCCCGCGGCCGAAGCGGATCTTCGAGATCCGCCCGGTGAAGTGGCTGCTGGAGAAAGGCACCATCGTCATCTGCGC 746 1 570 .......................................................................................... 659 2 606 .......................................................................................... 517 0 456 .......................................................................................... 545 A G G G G I P T M Y D E A G K K L S G V E A V I D K D L C S Query_1 747 TGGCGGCGGCGGTATCCCGACCATGTACGACGAGGCCGGGAAGAAGCTCAGCGGCGTCGAGGCGGTGATCGACAAGGACCTCTGCTCCTC 836 1 660 .......................................................................................... 749 2 516 .......................................................................................... 427 0 546 .......................................................................................... 635 S L L A Q E L V A D I L I I A T D V D A A Y V D W G K P T Q Query_1 837 GCTGCTGGCCCAGGAGCTGGTGGCCGACATCCTGATCATCGCCACCGACGTCGACGCGGCCTACGTCGACTGGGGCAAGCCGACCCAGAA 926 1 750 .......................................................................................... 839 2 426 .......................................................................................... 337 0 636 .......................................................................................... 725 K A I A Q A H P D E L E R L G F A A G S M G P K V Q A A I E Query_1 927 GGCCATCGCCCAGGCCCACCCGGACGAACTGGAGCGCCTCGGCTTCGCCGCCGGCTCCATGGGTCCGAAGGTCCAGGCCGCCATCGAGTT 1016 1 840 .......................................................................................... 929 2 336 .......................................................................................... 247 0 726 .......................................................................................... 815 F A R A T G K D A V I G S L A D I V A I T E G K A G T R V S Query_1 1017 CGCCCGCGCCACCGGCAAGGACGCGGTGATCGGTTCGCTCGCGGACATCGTCGCAATCACCGAGGGCAAGGCGGGAACACGGGTCAGC-A 1105 1 930 ..N...................................................N.................................N. 1019 2 246 ........................................................................................-. 158 0 816 ........................................................................................-. 904 T R K A G I E Y R * H W K W I T D P N S S S V D K L A A A L Query_1 1106 CGCGCAAGGCGGGCATCG-AGTACCGCTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTC 1194 1 1020 ......T...-.......A..........CAN......NN................A.......-......NN....G........N... 1107 2 157 ..................-....................................................................... 69 2 1238 .....N...... 1227 0 905 ..................-........ 930 E H H H H H H * D P A A N K A R K E A E L A A A T A E Q * Query_1 1195 GAGcaccaccaccacca-ccacTGA-GATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC 1282 1 1108 ....CA..-........C.......-.....-.........-.....NAN.G.N.....NGAN.N...................C.AT.. 1193 2 68 .................-.......G................................ 12 L A * P L G A S K R V L R G F L L K Query_1 1283 TAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAA 1335 1 1194 ....---NNN..NN...........--C.N....N.-A......G........ 1240 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4492514 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 3,454 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96670.102 (IDP96670.1-211.F..pMCSG53)
MAQTKTLTERAEALFGELHGLPLAERIEAINRIRRLLHAHSPFAEEPVDCVQWVRAERVAGNDYNPNTVAPPEMRLLRLSIESDGFTQPIVAYPQADGRYEVVDGFHRLRVGSAKGALRQRLHGYLPIAAIRAGREGDSERIAATIRHNRARGVHGVLPMTAIVVTLLKAGWSEADVARELGMDNDEVLRFKQVSGLPELFREHVYSRSWE
ATGGCACAGACAAAGACACTGACGGAGCGAGCCGAGGCGCTGTTCGGCGAACTGCACGGGTTGCCGCTTGCCGAGCGGATTGAAGCCATCAACCGGATCCGCCGGCTGCTCCACGCGCACAGTCCGTTCGCCGAAGAGCCGGTCGATTGCGTGCAGTGGGTGCGTGCCGAGCGCGTCGCCGGCAACGACTACAACCCCAATACCGTCGCGCCACCGGAGATGCGACTGCTGAGGCTGTCCATCGAGTCCGACGGATTCACCCAGCCCATCGTCGCCTATCCGCAGGCCGACGGGCGCTACGAGGTGGTCGACGGCTTTCACCGCCTGCGCGTGGGCAGCGCCAAGGGCGCGCTGCGCCAGCGTCTGCACGGCTACCTGCCGATCGCCGCGATACGCGCCGGGCGTGAAGGCGACAGCGAGCGCATCGCCGCCACCATCCGCCACAACCGAGCACGCGGCGTGCACGGCGTATTGCCGATGACGGCGATCGTGGTGACCCTGCTCAAGGCCGGCTGGAGCGAGGCCGACGTGGCCCGCGAACTGGGCATGGACAATGACGAGGTGCTGCGTTTCAAGCAGGTTTCCGGATTGCCCGAGCTGTTCCGCGAACACGTGTATTCCCGCTCCTGGGAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,995 total letters Query= IDP96670.102 Length=1038 Score E Sequences producing significant alignments: (Bits) Value 2_10A_A4_IDP96670.102_T7term.seq 1507 0.0 1_04A_A4_IDP96670.102_T7S.seq 1483 0.0 0_wt 1142 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTG--TGA-GCGGATAA-CAATTCCCCTCTAGAAATAATTTTGTTTAACT 86 2 920 .............................N.............--...A........A................................ 833 2 851 ...G..........TT.AT-C..CT.C.-..........A.. 890 1 22 .......................N... 48 L * E G D I H M H H H H H H S S G V D L W S H P Q F E K G T Query_1 87 TT--AAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACC 174 2 832 ..--.....A................................------------------------------------------------ 793 1 49 ..TA......................................------------------------------------------------ 90 E N L Y F Q S N A M A Q T K T L T E R A E A L F G E L H G L Query_1 175 GAGAACCTGTACTTCCAATCCAATGCCATGGCACAGACAAAGACACTGACGGAGCGAGCCGAGGCGCTGTTCGGCGAACTGCACGGGTTG 264 2 792 .......................................................................................... 703 1 91 .......................................................................................... 180 0 1 ............................................................... 63 P L A E R I E A I N R I R R L L H A H S P F A E E P V D C V Query_1 265 CCGCTTGCCGAGCGGATTGAAGCCATCAACCGGATCCGCCGGCTGCTCCACGCGCACAGTCCGTTCGCCGAAGAGCCGGTCGATTGCGTG 354 2 702 .......................................................................................... 613 1 181 .......................................................................................... 270 0 64 .......................................................................................... 153 Q W V R A E R V A G N D Y N P N T V A P P E M R L L R L S I Query_1 355 CAGTGGGTGCGTGCCGAGCGCGTCGCCGGCAACGACTACAACCCCAATACCGTCGCGCCACCGGAGATGCGACTGCTGAGGCTGTCCATC 444 2 612 .......................................................................................... 523 1 271 .......................................................................................... 360 0 154 .......................................................................................... 243 E S D G F T Q P I V A Y P Q A D G R Y E V V D G F H R L R V Query_1 445 GAGTCCGACGGATTCACCCAGCCCATCGTCGCCTATCCGCAGGCCGACGGGCGCTACGAGGTGGTCGACGGCTTTCACCGCCTGCGCGTG 534 2 522 .......................................................................................... 433 1 361 .......................................................................................... 450 0 244 .......................................................................................... 333 G S A K G A L R Q R L H G Y L P I A A I R A G R E G D S E R Query_1 535 GGCAGCGCCAAGGGCGCGCTGCGCCAGCGTCTGCACGGCTACCTGCCGATCGCCGCGATACGCGCCGGGCGTGAAGGCGACAGCGAGCGC 624 2 432 .......................................................................................... 343 1 451 .......................................................................................... 540 0 334 .......................................................................................... 423 I A A T I R H N R A R G V H G V L P M T A I V V T L L K A G Query_1 625 ATCGCCGCCACCATCCGCCACAACCGAGCACGCGGCGTGCACGGCGTATTGCCGATGACGGCGATCGTGGTGACCCTGCTCAAGGCCGGC 714 2 342 .......................................................................................... 253 1 541 .......................................................................................... 630 0 424 .......................................................................................... 513 W S E A D V A R E L G M D N D E V L R F K Q V S G L P E L Query_1 715 TGGAGCGAGGCCGACGTGGCCCGCGAACTGGGCATGGACAATGACGAGGTGCTGCGTTTCAAGCAGG-TTTCCGGATTGCCCGAGCTGTT 803 2 252 ...................................................................-...................... 164 1 631 ...................................................................T...................... 720 0 514 ...................................................................-...................... 602 F R E H V Y S R S W E * H W K W I T D P N S S S V D K L A Query_1 804 CCGCGAACACGTGTATT-CCCGCTCCTGGGAGTAACATTGGAAGT-GGATAACGGATCCGAA-TTCGAGCTCCGTCGACAAGCTTGCGGC 890 2 163 .................-...........................-................-........................... 77 1 721 .................C.................N.........G.......N........T...N....................... 810 0 603 .................-.............. 633 A A L E H H H H H H * D P A A N K A R K E A E L A A A T A Query_1 891 CGCACTCGAGcaccaccaccaccaccacTG-AGATCCGGCTGCT-AACAAAGCCCG-AAAGGAAGCTGAGTTGGCTGCTGCCA-CCGCTG 976 2 76 ..............................-.............A...........-...N... 15 1 811 ...N..........................A.............-...........A............N.............C...... 899 E Q * L A * P L G A S K R V L R G F L L K Query_1 977 AGCAATAACT-AGCATAA-CCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG-CTGAAA 1038 1 900 ..........A.N.....C..........T.N..........................C...... 964 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3017850 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 2,995 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96675.102 (IDP96675.1-218.F..pMCSG53)
MSSWKIAAAQYAPLNASPAEHVAHHLEYIELAARQQCELLVFPSLSLLGCDERNKPLPAPPDEALLQPLTHAADTHHMTIIVGMPVEHNCRFVKGIAIFAPWLTSPLMFHKSHGACIARQRSAINVVDEQPEGGDIDPSFTLFTTSQCLNEPELHASTSRLQRFSHKYALAVLMANACGSSALWDESGQLIVRADCGSLLLTGLRTTEGWQGDIIPLR
ATGTCCTCCTGGAAAATTGCCGCTGCACAGTACGCGCCCCTGAACGCTTCGCCGGCAGAGCATGTCGCCCATCATCTGGAATATATTGAGCTTGCGGCTCGTCAACAGTGCGAACTGCTGGTCTTTCCGTCACTGTCGCTATTAGGCTGCGACGAAAGAAATAAACCGTTGCCCGCCCCGCCTGACGAGGCGCTTTTACAGCCGCTCACTCATGCGGCCGATACCCACCATATGACTATCATTGTGGGTATGCCTGTTGAGCATAACTGCCGCTTCGTAAAAGGCATCGCTATTTTTGCCCCATGGCTAACGTCGCCGCTCATGTTTCACAAAAGTCATGGCGCCTGTATTGCCAGGCAGCGAAGCGCTATCAACGTGGTGGATGAGCAGCCAGAAGGTGGTGATATAGACCCCTCATTTACGTTATTCACAACCAGTCAATGTCTTAACGAGCCTGAACTCCATGCTTCTACCTCACGCTTACAACGTTTTTCGCATAAATATGCGCTGGCCGTACTGATGGCCAATGCCTGTGGCAGTAGCGCGCTGTGGGATGAAAGCGGGCAACTTATTGTTCGTGCCGATTGCGGTTCGCTATTACTGACGGGTCTGCGCACAACCGAGGGTTGGCAAGGCGATATCATTCCATTACGC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,148 total letters Query= IDP96675.102 Length=1059 Score E Sequences producing significant alignments: (Bits) Value 1_04H_H4_IDP96675.102_T7S.seq 1575 0.0 2_10H_H4_IDP96675.102_T7term.seq 1548 0.0 0_wt 1180 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L Query_1 1 TCTCGATCCCGCGAAATT-AATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTC-TAGAAATAATTTTGTTTAACTTT 88 1 20 ........C......NN............... 51 2 943 ..................W......................N........................-....................... 855 2 875 ...G...........T.T.C.C.C...N.......A-... 913 * E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E Query_1 89 AAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGA 178 1 52 .................................N....------------------------------------------------.... 93 2 854 ......................................------------------------------------------------.... 813 N L Y F Q S N A M S S W K I A A A Q Y A P L N A S P A E H V Query_1 179 ACCTGTACTTCCAATCCAATGCCATGTCCTCCTGGAAAA-TTGCCGCTGCACAGTACGCGCCCCTGAACGCTTCGCCGGCAGAGCATGTC 267 1 94 .......................................-.................................................. 182 2 812 .......................................A.................................................. 723 0 1 ................-.................................................. 66 A H H L E Y I E L A A R Q Q C E L L V F P S L S L L G C D E Query_1 268 GCCCATCATCTGGAATATATTGAGCTTGCGGCTCGTCAACAGTGCGAACTGCTGGTCTTTCCGTCACTGTCGCTATTAGGCTGCGACGAA 357 1 183 .......................................................................................... 272 2 722 .......................................................................................... 633 0 67 .......................................................................................... 156 R N K P L P A P P D E A L L Q P L T H A A D T H H M T I I V Query_1 358 AGAAATAAACCGTTGCCCGCCCCGCCTGACGAGGCGCTTTTACAGCCGCTCACTCATGCGGCCGATACCCACCATATGACTATCATTGTG 447 1 273 ....................................T..................................................... 362 2 632 ....................................T..................................................... 543 0 157 .......................................................................................... 246 G M P V E H N C R F V K G I A I F A P W L T S P L M F H K S Query_1 448 GGTATGCCTGTTGAGCATAACTGCCGCTTCGTAAAAGGCATCGCTATTTTTGCCCCATGGCTAACGTCGCCGCTCATGTTTCACAAAAGT 537 1 363 .......................................................................................... 452 2 542 .......................................................................................... 453 0 247 .......................................................................................... 336 H G A C I A R Q R S A I N V V D E Q P E G G D I D P S F T L Query_1 538 CATGGCGCCTGTATTGCCAGGCAGCGAAGCGCTATCAACGTGGTGGATGAGCAGCCAGAAGGTGGTGATATAGACCCCTCATTTACGTTA 627 1 453 .......................................................................................... 542 2 452 .......................................................................................... 363 0 337 .......................................................................................... 426 F T T S Q C L N E P E L H A S T S R L Q R F S H K Y A L A V Query_1 628 TTCACAACCAGTCAATGTCTTAACGAGCCTGAACTCCATGCTTCTACCTCACGCTTACAACGTTTTTCGCATAAATATGCGCTGGCCGTA 717 1 543 .......................................................................................... 632 2 362 .......................................................................................... 273 0 427 .......................................................................................... 516 L M A N A C G S S A L W D E S G Q L I V R A D C G S L L L Query_1 718 CTGATGGCCAATGCCTGTGGCAGTAGCGCGCTGTGGGATGAAAGCGGGCAACTTATTGTTCGTG-CCGATTGCGG-TTCGCTATTACTGA 805 1 633 ................................................................C..........T.............. 722 2 272 ................................................................-..........-.............. 185 0 517 ................................................................-..........-.............. 604 T G L R T T E G W Q G D I I P L R * H W K W I T D P N S S S Query_1 806 CGGGTCTGCGCACAACCGAGGGTTGGCAAGGCGATATCATTCCATTACGCTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCG 895 1 723 .......................................................................................... 812 2 184 .......................................................................................... 95 0 605 .................................................. 654 V D K L A A A L E H H H H H H * D P A A N K A R K E A E L Query_1 896 TCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCG--AAAGGAAGCTGAGTTGGC 983 1 813 ......................................................................--..............N... 900 2 94 ......................................................................AA........ 15 A A A T A E Q * L A * P L G A S K R V L R G F L L Query_1 984 TGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTC-TTGAGGGGTTTTTTGCTGAA 1058 1 901 ..............................................-........T.................... 975 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3233300 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 3,148 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96708.102 (IDP96708.1-253.F..pMCSG53)
MTKVAIVTASDSGIGKACALLLAQNGFDIGITWHSDERGAQETAKKAAQFGVRAETIHLDLSQLPEGAQAIEHLIQRLGRVDVLVNNAGAMTKSAFIDMPFTQWRQIFTVDVDGAFLCAQIAARHMIKQGEGGRIINITSVHEHTPLPQASAYTAAKHALGGLTKSMALELIEHHILVNAVAPGAIATPMNDMDDSDIEPGSEPSIPIARPGSTHEIASLVAWLCSEGASYTTGQSLIVDGGFMLANPQFNAK
ATGACCAAAGTCGCAATTGTAACGGCATCAGATTCGGGAATTGGCAAAGCGTGTGCGTTGTTATTAGCACAAAACGGTTTTGATATTGGTATCACCTGGCATTCCGATGAACGCGGCGCGCAGGAGACGGCAAAAAAAGCGGCGCAGTTTGGTGTGCGGGCAGAGACCATTCATCTTGACCTGAGCCAGTTACCCGAGGGCGCGCAGGCCATTGAACATCTTATTCAGCGCCTGGGGCGCGTTGACGTGCTGGTCAATAATGCCGGGGCGATGACCAAATCCGCGTTTATTGATATGCCGTTTACGCAGTGGCGGCAAATTTTTACCGTGGACGTCGATGGCGCGTTTCTCTGCGCGCAGATCGCCGCCCGGCATATGATTAAACAAGGAGAGGGAGGGCGCATTATCAACATCACCTCCGTCCATGAGCATACGCCGCTTCCGCAAGCCAGCGCCTATACCGCGGCGAAACATGCGTTGGGGGGATTGACTAAATCCATGGCGCTGGAACTGATTGAGCATCATATTTTGGTCAACGCCGTCGCGCCAGGCGCTATCGCCACGCCGATGAATGATATGGATGACAGCGACATTGAGCCGGGATCGGAACCCTCAATCCCTATCGCCCGACCAGGTTCTACGCATGAAATAGCCAGCCTCGTCGCCTGGCTGTGTTCGGAAGGAGCAAGCTACACCACCGGTCAGTCGCTGATTGTCGACGGCGGGTTTATGCTGGCGAATCCGCAATTCAACGCAAAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,254 total letters Query= IDP96708.102 Length=1164 Score E Sequences producing significant alignments: (Bits) Value 2_11A_A5_IDP96708.102_T7term.seq 1752 0.0 1_05A_A5_IDP96708.102_T7S.seq 1721 0.0 0_wt 1370 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAA 90 2 1046 .........................................................................................G 957 2 979 ...G...........T.T.C.C.C...........A... 1017 1 14 ..T.............-................... 48 E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E N Query_1 91 GAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAAC 180 2 956 ..G.................................------------------------------------------------...... 915 1 49 ....................................------------------------------------------------...... 90 L Y F Q S N A M T K V A I V T A S D S G I G K A C A L L L A Query_1 181 CTGTACTTCCAATCCAATGCCATGACCAAAGTCGCAATTGTAACGGCATCAGATTCGGGAATTGGCAAAGCGTGTGCGTTGTTATTAGCA 270 2 914 .......................................................................................... 825 1 91 .......................................................................................... 180 0 1 ..................................................................... 69 Q N G F D I G I T W H S D E R G A Q E T A K K A A Q F G V R Query_1 271 CAAAACGGTTTTGATATTGGTATCACCTGGCATTCCGATGAACGCGGCGCGCAGGAGACGGCaaaaaaaGCGGCGCAGTTTGGTGTGCGG 360 2 824 .......................................................................................... 735 1 181 .......................................................................................... 270 0 70 .......................................................................................... 159 A E T I H L D L S Q L P E G A Q A I E H L I Q R L G R V D V Query_1 361 GCAGAGACCATTCATCTTGACCTGAGCCAGTTACCCGAGGGCGCGCAGGCCATTGAACATCTTATTCAGCGCCTGGGGCGCGTTGACGTG 450 2 734 .......................................................................................... 645 1 271 .......................................................................................... 360 0 160 .......................................................................................... 249 L V N N A G A M T K S A F I D M P F T Q W R Q I F T V D V D Query_1 451 CTGGTCAATAATGCCGGGGCGATGACCAAATCCGCGTTTATTGATATGCCGTTTACGCAGTGGCGGCAAATTTTTACCGTGGACGTCGAT 540 2 644 .......................................................................................... 555 1 361 .......................................................................................... 450 0 250 .......................................................................................... 339 G A F L C A Q I A A R H M I K Q G E G G R I I N I T S V H E Query_1 541 GGCGCGTTTCTCTGCGCGCAGATCGCCGCCCGGCATATGATTAAACAAGGAGAGGGAGGGCGCATTATCAACATCACCTCCGTCCATGAG 630 2 554 .......................................................................................... 465 2 533 ............ 544 1 451 .......................................................................................... 540 1 472 ............ 461 0 340 .......................................................................................... 429 0 361 ............ 350 H T P L P Q A S A Y T A A K H A L G G L T K S M A L E L I E Query_1 631 CATACGCCGCTTCCGCAAGCCAGCGCCTATACCGCGGCGAAACATGCGTTGGGGGGATTGACTAAATCCATGGCGCTGGAACTGATTGAG 720 2 464 .......................................................................................... 375 1 541 .......................................................................................... 630 0 430 .......................................................................................... 519 H H I L V N A V A P G A I A T P M N D M D D S D I E P G S E Query_1 721 CATCATATTTTGGTCAACGCCGTCGCGCCAGGCGCTATCGCCACGCCGATGAATGATATGGATGACAGCGACATTGAGCCGGGATCGGAA 810 2 374 .......................................................................................... 285 1 631 .......................................................................................... 720 0 520 .......................................................................................... 609 P S I P I A R P G S T H E I A S L V A W L C S E G A S Y T Query_1 811 -CCCTCAATCCCTATCGCCCGACCAGGTTCTACGCATGAAATAGCCAGCCTCGTCGCCTGGCTGTGTTCGGAAGGAGCAAGCTACACCAC 899 2 284 -......................................................................................... 196 1 721 N......................................................................................... 810 0 610 -......................................................................................... 698 T G Q S L I V D G G F M L A N P Q F N A K * H W K W I T D P Query_1 900 CGGTCAGTCGCTGATTGTCGACGGCGGGTTTATGCTGGCGAATCCGCAATTCAACGCAAAATAACATTGGAAGTGGATAACGG-ATCCGA 988 2 195 ...................................................................................-...... 107 1 811 ...............................................................CAT.................A....N. 900 0 699 ............................................................. 759 N S S S V D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 989 ATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCG-AAAGGAA 1077 2 106 ..................................................................................A....... 17 1 901 .................N................................................................-.....-. 988 A E L A A A T A E Q * L A * P L G A S K R V L R G F L L Query_1 1078 GCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAA 1163 2 16 . 16 1 989 ............................-.............-..............-.....TNN.......--........... 1069 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3680488 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:10 AM Number of letters in database: 3,254 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96728.102 (IDP96728.1-199.F..pMCSG53)
MVILFSGNIMAATNMTDNVTLNNDKISGQAWQAMRDIGMSRFELFNGRTQKAEQLAAQAEKLLNDDSTDWNLYVKSDKKAPVEGDHYIRINSSITVAEDYLPAGQKNDAINKANQKMKEGDKKGTIEALKLAGVSVIENQELIPLQQTRKDVTTALSLMNEGKYYQAGLLLKSAQDGIVVDSQSVQESPTHSVQHDAAH
ATGGTTATCCTGTTTTCAGGAAATATTATGGCCGCAACAAACATGACTGATAACGTTACTCTGAATAATGACAAGATATCCGGACAGGCCTGGCAGGCCATGCGTGATATCGGAATGTCGCGATTCGAGTTGTTTAATGGTCGCACCCAAAAGGCGGAACAACTGGCTGCGCAAGCAGAAAAACTCCTGAACGATGACAGCACTGACTGGAATCTGTATGTCAAATCAGATAAAAAAGCCCCAGTTGAAGGAGACCACTATATTCGGATAAATTCATCGATTACGGTAGCAGAAGATTATCTCCCTGCGGGCCAGAAAAATGATGCTATTAATAAAGCAAACCAGAAAATGAAAGAAGGAGATAAAAAAGGAACTATAGAGGCCCTGAAACTAGCAGGCGTCAGTGTTATTGAAAATCAGGAACTCATTCCTCTTCAGCAAACCCGTAAGGATGTGACCACAGCGCTTTCCTTGATGAACGAAGGAAAATACTATCAGGCAGGTCTGTTACTGAAATCAGCTCAGGATGGCATAGTTGTAGACTCTCAGTCAGTTCAGGAATCACCCACGCACTCCGTGCAGCACGACGCCGCGCAT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,091 total letters Query= IDP96728.102 Length=1002 Score E Sequences producing significant alignments: (Bits) Value 1_05B_B5_IDP96728.102_T7S.seq 1507 0.0 2_11B_B5_IDP96728.102_T7term.seq 1442 0.0 0_wt 1077 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGA-GCGG-ATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTT 88 1 15 ..T..........-................. 44 2 889 ..............................................-....G...................................... 801 2 821 ...G...........T.TC.C.-C.C...........A... 860 * E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E Query_1 89 AAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGA 178 1 45 ......................................------------------------------------------------.... 86 1 462 ............ 473 2 800 ......................................------------------------------------------------.... 759 2 383 ............ 372 0 353 ............ 364 N L Y F Q S N A M V I L F S G N I M A A T N M T D N V T L N Query_1 179 ACCTGTACTTCCAATCCAATGCCATGGTTATCCTGTTTTCAGGAAATATTATGGCCGCAACAAACATGACTGATAACGTTACTCTGAATA 268 1 87 .......................................................................................... 176 2 758 .......................................................................................... 669 0 1 ................................................................... 67 N D K I S G Q A W Q A M R D I G M S R F E L F N G R T Q K A Query_1 269 ATGACAAGATATCCGGACAGGCCTGGCAGGCCATGCGTGATATCGGAATGTCGCGATTCGAGTTGTTTAATGGTCGCACCCAAAAGGCGG 358 1 177 .......................................................................................... 266 2 668 .......................................................................................... 579 0 68 .......................................................................................... 157 E Q L A A Q A E K L L N D D S T D W N L Y V K S D K K A P V Query_1 359 AACAACTGGCTGCGCAAGCAGAAAAACTCCTGAACGATGACAGCACTGACTGGAATCTGTATGTCAAATCAGATAAAAAAGCCCCAGTTG 448 1 267 .......................................................................................... 356 1 469 ........... 479 2 578 .......................................................................................... 489 2 376 ........... 366 0 158 .......................................................................................... 247 0 360 ........... 370 E G D H Y I R I N S S I T V A E D Y L P A G Q K N D A I N K Query_1 449 AAGGAGACCACTATATTCGGATAAATTCATCGATTACGGTAGCAGAAGATTATCTCCCTGCGGGCCAGAAAAATGATGCTATTAATAAAG 538 1 357 .......................................................................................... 446 2 488 .......................................................................................... 399 0 248 .......................................................................................... 337 A N Q K M K E G D K K G T I E A L K L A G V S V I E N Q E L Query_1 539 CAAACCAGAAAATGAAAGAAGGAGATAAAAAAGGAACTATAGAGGCCCTGAAACTAGCAGGCGTCAGTGTTATTGAAAATCAGGAACTCA 628 1 447 .......................................................................................... 536 1 45 ............ 56 1 337 ........... 347 2 398 .......................................................................................... 309 2 800 ............ 789 2 508 ........... 498 0 338 .......................................................................................... 427 0 228 ........... 238 I P L Q Q T R K D V T T A L S L M N E G K Y Y Q A G L L L K Query_1 629 TTCCTCTTCAGCAAACCCGTAAGGATGTGACCACAGCGCTTTCCTTGATGAACGAAGGAAAATACTATCAGGCAGGTCTGTTACTGAAAT 718 1 537 .......................................................................................... 626 2 308 .......................................................................................... 219 0 428 .......................................................................................... 517 S A Q D G I V V D S Q S V Q E S P T H S V Q H D A A H * H W Query_1 719 CAGCTCAGGATGGCATAGTTGTAGACTCTCAGTCAGTTCAGGAATCACCCACGCACTCCGTGCAGCACGACGCCGCGCATTAACATTGGA 808 1 627 .......................................................................................... 716 2 218 .......................................................................................... 129 0 518 ................................................................................ 597 K W I T D P N S S S V D K L A A A L E H H H H H H * D P A A Query_1 809 AGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCT- 897 1 717 .........................................................................................- 805 2 128 .........................................................................................A 39 N K A R K E A E L A A A T A E Q * L A * P L G A S K R V L Query_1 898 AACAAA---GCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTG 984 1 806 ...-..---................................................................................. 891 2 38 ......AGCC...N....... 18 R G F L L K Query_1 985 AGGGGTTTTTTGCTGAAA 1002 1 892 .................. 909 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3006402 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 3,091 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96733.102 (IDP96733.22-207.P(207)..pMCSG53)
ADNAPVAAQQQTQQTQKTAAAERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN
GCTGATAATGCACCGGTAGCGGCACAACAGCAAACTCAGCAAACCCAGAAAACGGCAGCAGCAGAGCGTATCTCTGAACAAGGTTTATATGCGATGCGTGACGTGCAGGTAGCGCGCCTGGCTCTGTTTCATGGCGATCCGGAAAAGGCAAAAGAACTGACCAATGAAGCTTCCGCTTTGCTGTCAGATGACAGTACCGAATGGGCAAAGTTCGCTAAGCCAGGTAAAAAGACCAATCTCAATGATGACCAATATATTGTCATTAATGCATCTGTCGGAATTTCTGAGAGTTATGTAGCAACACCCGAAAAAGAAGCCGCGATAAAAATTGCCAATGAAAAAATGGCGAAAGGGGATAAAAAGGGCGCAATGGAAGAGCTTCGCCTGGCCGGTGTTGGCGTAATGGAAAACCAATATCTGATGCCGCTTAAACAGACTCGTAATGCACTTGCTGATGCGCAGAAACTGCTTGATAAAAAGCAATACTACGAGGCAAACCTGGCGCTTAAAGGTGCGGAAGATGGAATCATCGTCGATAGCGAAGCACTGTTTGTGAAC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,186 total letters Query= IDP96733.102 Length=963 Score E Sequences producing significant alignments: (Bits) Value 2_10A_A7_IDP96733.102_T7term.seq 1379 0.0 1_01B_A7_IDP96733.102_T7short.seq 1216 0.0 0_wt 1007 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAA 90 2 842 .......................................................................................... 753 2 775 ...G...........T.T.C.C.C...........A... 813 1 19 ..........-.....- 33 E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E N Query_1 91 GAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAAC 180 2 752 ....................................------------------------------------------------...... 711 1 70 ...... 75 1 34 .................................... 69 L Y F Q S N A A D N A P V A A Q Q Q T Q Q T Q K T A A A E Query_1 181 CTGTACTTCCAATCCAATGCCGCTGATAATGCACCGGTAGCGGCACAACA-GCAAACTCAGCAAACCCAGAAAACGGCAGCAGCAGAGCG 269 2 710 ..................................................N....................................... 621 1 76 ..................................................-....................................... 164 0 1 .............................-....................................... 68 R I S E Q G L Y A M R D V Q V A R L A L F H G D P E K A K E Query_1 270 TATCTCTGAACAAGGTTTATATGCGATGCGTGACGTGCAGGTAGCGCGCCTGGCTCTGTTTCATGGCGATCCGGAAAAGGCAAAAGAACT 359 2 620 .......................................................................................... 531 1 165 .......................................................................................... 254 0 69 .......................................................................................... 158 L T N E A S A L L S D D S T E W A K F A K P G K K T N L N D Query_1 360 GACCAATGAAGCTTCCGCTTTGCTGTCAGATGACAGTACCGAATGGGCAAAGTTCGCTAAGCCAGGTAAAAAGACCAATCTCAATGATGA 449 2 530 .......................................................................................... 441 1 255 ............................................................................N............. 344 0 159 .......................................................................................... 248 D Q Y I V I N A S V G I S E S Y V A T P E K E A A I K I A N Query_1 450 CCAATATATTGTCATTAATGCATCTGTCGGAATTTCTGAGAGTTATGTAGCAACACCCgaaaaagaagccgcgataaaaattgccaatga 539 2 440 .......................................................................................... 351 1 345 .......................................................................................... 434 0 249 .......................................................................................... 338 E K M A K G D K K G A M E E L R L A G V G V M E N Q Y L M P Query_1 540 aaaaatggcgaaaggggataaaaagGGCGCAATGGAAGAGCTTCGCCTGGCCGGTGTTGGCGTAATGGAAAACCAATATCTGATGCCGCT 629 2 350 .......................................................................................... 261 1 435 ...........................................................................NN............. 524 0 339 .......................................................................................... 428 L K Q T R N A L A D A Q K L L D K K Q Y Y E A N L A L K G A Query_1 630 TAAACAGACTCGTAATGCACTTGCTGATGCGCAGAAACTGCTTGATAAAAAGCAATACTACGAGGCAAACCTGG-CGCTTAAAGGTGCGG 718 2 260 ..........................................................................-............... 172 1 525 ...................................................N......................T............... 614 0 429 ..........................................................................-............... 517 E D G I I V D S E A L F V N * H W K W I T D P N S S S V D K Query_1 719 AAGATGGAATCATCGTCGATAGCGAAGCACTGTTTGTGAACTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCT-CCGTCGACAAG 807 2 171 ..............................................................................-........... 83 1 615 ...........................................N.....................N.......N....C....N....GC 704 0 518 ......................................... 558 L A A A L E H H H H H H * D P A A N K A R K E A E L A A Query_1 808 CTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGAT-CCGGCTGCTAACAAAG-CCCGAAAGGAA-GCTGAG-TTGGCT-GCTG 892 2 82 ..........................................-........N.......C....... 17 1 705 ....N.......N..T......N.....N..........N..C....N..........N-...NN......N......T......G.... 793 A T A E Q * L A * P L G A S K R V L R G F Query_1 893 CCA--CCGC-TGAGC-AATAACT-AGCATAA--CCCCTTGGGG-CCTCTAAACGGG-TCTTGAGGGGTTTTTT 956 1 794 ..NNT.N..T.....T.T..N..A..N....CC.........NC............T.N..T.N......... 866 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2977668 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 3,186 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96744.102 (IDP96744.1-810.F..pMCSG53)
MTQLKLDTLSDRIKAHKTALVHIVKPPVCTERAQHYTEMYQQHLDKPIPVRRALALAHHLAERTIWIKHDELIVGNQASEVRAAPIFPEYTVSWIEKEIDDLADRPGAGFSVSEENKRILHDVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMTSGDAHLAVNFPLLLEKGLYGLRDKVAERRSRINLTVLEDLHGEQFLKAIDIVLDAVSQHITRFAALARQMAGEESRESRRKELLTIAENCEVIAHQPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSHSKASAGSPLYQNVTIGGQKLINGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKWGYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMAAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGIANLGNSLAAVKKLVFEQGVIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDTVDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAATMATPDGRKAHTPLAEGASPASGTDHLGPTAVIGSVGKLPTGSILGGVLLNQKLNPTTLENESDKQKLMVLLRTFFEVHKGWHIQYNIVSRETLLDAKKHPDQYRDLVVRVAGYSAFFTALSPDAQDDIIARTEHML
ATGACCCAACTGAAACTGGACACGCTCAGTGACCGCATTAAGGCGCACAAAACCGCGCTGGTACATATCGTGAAACCGCCGGTCTGTACCGAGCGCGCGCAGCATTATACCGAAATGTACCAGCAGCATCTGGATAAACCGATTCCGGTACGCCGTGCGCTGGCGCTGGCCCATCACCTGGCGGAACGCACTATCTGGATAAAACACGATGAACTGATCGTCGGCAACCAGGCAAGTGAAGTCCGCGCCGCGCCGATTTTCCCGGAATATACCGTCTCGTGGATTGAAAAAGAGATCGACGACCTGGCGGACCGGCCCGGCGCAGGTTTTTCCGTAAGTGAAGAAAACAAACGTATTCTGCACGACGTCTGTCCGTGGTGGCGCGGTCAAACCGTCCAGGATCGCTGCTACGGCATGTTTACCGATGAACAAAAAGGGCTTCTGGCGACTGGCATTATCAAAGCCGAAGGCAATATGACTTCCGGCGATGCGCACCTGGCGGTGAACTTCCCGCTGCTGCTGGAAAAAGGGCTTTATGGCCTGCGCGATAAAGTCGCCGAGCGCCGCTCACGCATCAACCTGACCGTGCTGGAAGATCTGCATGGCGAGCAGTTCCTGAAGGCGATTGATATTGTGCTGGACGCGGTAAGCCAGCATATCACGCGCTTTGCCGCGCTGGCGCGCCAGATGGCGGGCGAAGAGTCCCGTGAAAGCCGTCGTAAAGAGCTACTTACCATCGCCGAAAACTGCGAGGTGATCGCCCACCAACCGCCGCAGACCTTCTGGCAAGCACTGCAATTGTGCTACTTCATCCAACTGATTCTACAAATTGAGTCTAACGGTCACTCGGTGTCATTTGGTCGTATGGACCAGTATCTTTACCCCTATTATCGTCGCGATGTCGAACTAAACCAGACGCTGGATCGTGAGCACGCCATTGAACTGCTGCACAGCTGCTGGCTGAAACTGCTGGAAGTAAACAAGATCCGCTCCGGTTCGCACTCTAAAGCCTCCGCGGGCAGCCCGCTGTATCAAAACGTCACCATTGGCGGTCAGAAACTTATCAACGGTCAGCCAATGGATGCAGTGAATCCGCTGTCTTACGCCATTCTGGAGTCCTGTGGACGTCTGCGTTCTACCCAGCCCAACCTCAGTGTGCGTTATCACGCCGGAATGAGTAACGATTTCCTTGACGCCTGCGTCCAGGTCATCCGCTGCGGCTTTGGGATGCCGGCGTTTAACAATGATGAAATCGTCATCCCGGAATTTATCAAGTTGGGGATCGAACCGCAGGACGCTTACGATTATGCGGCGATCGGCTGTATCGAAACCGCCGTCGGCGGGAAATGGGGTTATCGCTGCACCGGCATGAGCTTTATTAACTTCGCCCGCGTCATGCTGGCGGCGCTGGAAGGCGGTCGCGACGCCACCAGCGGCAAGGTATTTCTGCCGCAGGAAAAGGCGCTCTCCGCAGGCAACTTTAACAATTTTGACGAAGTGATGGCCGCCTGGGATACCCAAATTCGCTACTACACGCGCAAATCGATTGAAATTGAGTATGTGGTCGACACCATGTTGGAAGAGAACGTCCACGATATCCTCTGCTCAGCGCTGGTAGATGACTGTATTGAGCGAGCGAAAAGTATCAAGCAAGGCGGCGCGAAATATGACTGGGTATCCGGTCTCCAGGTCGGTATCGCCAACCTGGGCAACAGCCTCGCCGCTGTGAAAAAACTGGTCTTCGAACAGGGCGTTATCGGTCAACAGCAGCTTGCCGCCGCGCTGGCCGATGACTTTGACGGGCTGACCCATGAGCAGCTGCGCCAGCGTCTGATTAACGGCGCGCCGAAGTACGGTAACGACGATGATACCGTCGATACGCTGCTGGCGCGCGCTTATCAGACCTATATTGATGAGCTGAAGCAATATCATAACCCGCGCTACGGTCGTGGCCCTGTCGGCGGCAACTATTACGCCGGTACGTCCTCTATCTCAGCAAACGTGCCATTTGGCGCCGCGACTATGGCTACCCCGGATGGTCGCAAAGCGCATACACCGCTGGCGGAAGGCGCAAGCCCTGCATCAGGTACGGATCATCTCGGCCCGACGGCGGTGATTGGTTCCGTAGGCAAATTACCTACCGGTTCCATTCTCGGCGGCGTGCTGCTCAACCAGAAACTGAACCCAACGACGCTGGAAAACGAATCCGACAAACAGAAACTGATGGTTCTGCTACGGACGTTCTTTGAGGTGCATAAAGGCTGGCATATTCAGTACAACATCGTGTCACGCGAAACGCTGTTGGATGCGAAAAAACATCCAGACCAGTACCGCGATCTGGTCGTGCGTGTGGCAGGCTACTCCGCCTTCTTCACCGCGCTGTCGCCGGATGCGCAGGACGATATCATCGCCCGTACCGAACATATGCTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,316 total letters Query= IDP96744.102 Length=2835 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4383 0.0 2_11C_C5_IDP96744.102_T7term.seq 1606 0.0 1_05C_C5_IDP96744.102_T7S.seq 1445 0.0 Q F P S R N N F V * L * E G D I H M H H H H H H S S G V D Query_1 55 CAATTCCCC-TCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCT 143 1 17 ..T......N.......-.......................................................----------------- 88 L W S H P Q F E K G T E N L Y F Q S N A M T Q L K L D T L S Query_1 144 GTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGACCCAACTGAAACTGGACACGCTCAGTGA 233 0 1 ................................ 32 1 89 -------------------------------........................................................... 147 D R I K A H K T A L V H I V K P P V C T E R A Q H Y T E M Y Query_1 234 CCGCATTAAGGCGCACAAAACCGCGCTGGTACATATCGTGAAACCGCCGGTCTGTACCGAGCGCGCGCAGCATTATACCGAAATGTACCA 323 0 33 .......................................................................................... 122 1 148 .......................................................................................... 237 Q Q H L D K P I P V R R A L A L A H H L A E R T I W I K H D Query_1 324 GCAGCATCTGGATAAACCGATTCCGGTACGCCGTGCGCTGGCGCTGGCCCATCACCTGGCGGAACGCACTATCTGGATAAAACACGATGA 413 0 123 .......................................................................................... 212 0 128 ........... 138 0 193 ........... 203 1 238 .......................................................................................... 327 1 243 ........... 253 E L I V G N Q A S E V R A A P I F P E Y T V S W I E K E I D Query_1 414 ACTGATCGTCGGCAACCAGGCAAGTGAAGTCCGCGCCGCGCCGATTTTCCCGGAATATACCGTCTCGTGGATTGAAAAAGAGATCGACGA 503 0 213 .......................................................................................... 302 1 328 .......................................................................................... 417 D L A D R P G A G F S V S E E N K R I L H D V C P W W R G Q Query_1 504 CCTGGCGGACCGGCCCGGCGCAGGTTTTTCCGTAAGTGAAGAAAACAAACGTATTCTGCACGACGTCTGTCCGTGGTGGCGCGGTCAAAC 593 0 303 .......................................................................................... 392 1 418 .......................................................................................... 507 T V Q D R C Y G M F T D E Q K G L L A T G I I K A E G N M T Query_1 594 CGTCCAGGATCGCTGCTACGGCATGTTTACCGATGAACAAAAAGGGCTTCTGGCGACTGGCATTATCAAAGCCGAAGGCAATATGACTTC 683 0 393 .......................................................................................... 482 0 524 ........... 534 1 508 .......................................................................................... 597 S G D A H L A V N F P L L L E K G L Y G L R D K V A E R R S Query_1 684 CGGCGATGCGCACCTGGCGGTGAACTTCCCGCTGCTGCTGGAAAAAGGGCTTTATGGCCTGCGCGATAAAGTCGCCGAGCGCCGCTCACG 773 0 483 .......................................................................................... 572 0 431 ........... 441 1 598 ................................................................N......................... 687 1 546 ........... 556 R I N L T V L E D L H G E Q F L K A I D I V L D A V S Q H I Query_1 774 CATCAACCTGACCGTGCT-GGAAGATCTGCATGGCGAGCAGTTCCTGAAGGCGATTGATATTGTGCTGGACGCGGTAAGCCAGCATATCA 862 0 573 ..................-....................................................................... 661 1 688 ..................G....................................................................... 777 T R F A A L A R Q M A G E E S R E S R R K E L L T I A E N Query_1 863 CGCGC-TTTGCCGCGCTGGCGCGCCAGATGGC-GGGCGAAGAGTCCCGTGAAAGCCGTCGTAAAGAGCTACTTACCATCGCCG-AAAACT 949 0 662 .....-..........................-..................................................-...... 748 0 687 ............ 676 0 1777 ........... 1787 1 778 .....T....N...............N.....G.............................N....................A...... 867 1 803 ........... 793 C E V I A H Q P P Q T F W Q A L Q L C Y F I Q L I L Q I E S Query_1 950 GCGAGGTGATCGCCCACCAACCGCCGCAGACC-TTCTGGCAAGCACTGCAATTGTGCTACTTCATCCAACTGATTCTACAAATTGAGTCT 1038 0 749 ................................-......................................................... 837 1 868 ......N...........-.N.........N.T.....N.....N........... 922 N G H S V S F G R M D Q Y L Y P Y Y R R D V E L N Q T L D R Query_1 1039 AACGGTCACTCGGTGTCATTTGGTCGTATGGACCAGTATCTTTACCCCTATTATCGTCGCGATGTCGAACTAAACCAGACGCTGGATCGT 1128 0 838 .......................................................................................... 927 E H A I E L L H S C W L K L L E V N K I R S G S H S K A S A Query_1 1129 GAGCACGCCATTGAACTGCTGCACAGCTGCTGGCTGAAACTGCTGGAAGTAAACAAGATCCGCTCCGGTTCGCACTCTAAAGCCTCCGCG 1218 0 928 .......................................................................................... 1017 G S P L Y Q N V T I G G Q K L I N G Q P M D A V N P L S Y A Query_1 1219 GGCAGCCCGCTGTATCAAAACGTCACCATTGGCGGTCAGAAACTTATCAACGGTCAGCCAATGGATGCAGTGAATCCGCTGTCTTACGCC 1308 0 1018 .......................................................................................... 1107 I L E S C G R L R S T Q P N L S V R Y H A G M S N D F L D A Query_1 1309 ATTCTGGAGTCCTGTGGACGTCTGCGTTCTACCCAGCCCAACCTCAGTGTGCGTTATCACGCCGGAATGAGTAACGATTTCCTTGACGCC 1398 0 1108 .......................................................................................... 1197 C V Q V I R C G F G M P A F N N D E I V I P E F I K L G I E Query_1 1399 TGCGTCCAGGTCATCCGCTGCGGCTTTGGGATGCCGGCGTTTAACAATGATGAAATCGTCATCCCGGAATTTATCAAGTTGGGGATCGAA 1488 0 1198 .......................................................................................... 1287 P Q D A Y D Y A A I G C I E T A V G G K W G Y R C T G M S F Query_1 1489 CCGCAGGACGCTTACGATTATGCGGCGATCGGCTGTATCGAAACCGCCGTCGGCGGGAAATGGGGTTATCGCTGCACCGGCATGAGCTTT 1578 0 1288 .......................................................................................... 1377 0 2114 ........... 2104 2 451 ........... 461 I N F A R V M L A A L E G G R D A T S G K V F L P Q E K A L Query_1 1579 ATTAACTTCGCCCGCGTCATGCTGGCGGCGCTGGAAGGCGGTCGCGACGCCACCAGCGGCAAGGTATTTCTGCCGCAGGAAAAGGCGCTC 1668 0 1378 .......................................................................................... 1467 S A G N F N N F D E V M A A W D T Q I R Y Y T R K S I E I E Query_1 1669 TCCGCAGGCAACTTTAACAATTTTGACGAAGTGATGGCCGCCTGGGATACCCAAATTCGCTACTACACGCGCAAATCGATTGAAATTGAG 1758 0 1468 .......................................................................................... 1557 Y V V D T M L E E N V H D I L C S A L V D D C I E R A K S Query_1 1759 TATGTGGTCGACACCATGTTGGAAGAGAACGTCCACGATATCCTCTGCTCAGCGCTGGTAG-ATGACTGTATTGAGCGAG-CGAAAAGTA 1846 0 1558 .............................................................-..................-......... 1645 2 964 ..........-...N.............N....G......... 923 I K Q G G A K Y D W V S G L Q V G I A N L G N S L A A V K Query_1 1847 TCAAGCAAGGCGGCGCGAAA-TATGACTGGGTATCCGGTCT-CCAGGTCGGTATCGCCAACCTGGGCAACAGCCTCGCCGCTGTGAAAAA 1934 0 1646 ....................-....................-................................................ 1733 2 922 .........-..........A....................T....T........................................... 834 K L V F E Q G V I G Q Q Q L A A A L A D D F D G L T H E Q L Query_1 1935 ACTGGT-CTTCGAACAGGGCGTTATCGGTCAACAGCAGCTTGCCGCC-GCGCTGGCCGATGACTTTGACGGGCTGACCCATGAGCAGCTG 2022 0 1734 ......-........................................-.......................................... 1821 0 670 ...-........ 680 2 833 .N.N..TN..........N............................C.......................................... 744 R Q R L I N G A P K Y G N D D D T V D T L L A R A Y Q T Y I Query_1 2023 CGCCAGCGTCTGATTAACGGCGCGCCGAAGTACGGTAACGACGATGATACCGTCGATACGCTGCTGGCGCGCGCTTATCAGACCTATATT 2112 0 1822 .......................................................................................... 1911 2 743 .......................................................................................... 654 D E L K Q Y H N P R Y G R G P V G G N Y Y A G T S S I S A N Query_1 2113 GATGAGCTGAAGCAATATCATAACCCGCGCTACGGTCGTGGCCCTGTCGGCGGCAACTATTACGCCGGTACGTCCTCTATCTCAGCAAAC 2202 0 1912 .......................................................................................... 2001 2 653 .......................................................................................... 564 V P F G A A T M A T P D G R K A H T P L A E G A S P A S G T Query_1 2203 GTGCCATTTGGCGCCGCGACTATGGCTACCCCGGATGGTCGCAAAGCGCATACACCGCTGGCGGAAGGCGCAAGCCCTGCATCAGGTACG 2292 0 2002 .......................................................................................... 2091 2 563 .......................................................................................... 474 D H L G P T A V I G S V G K L P T G S I L G G V L L N Q K L Query_1 2293 GATCATCTCGGCCCGACGGCGGTGATTGGTTCCGTAGGCAAATTACCTACCGGTTCCATTCTCGGCGGCGTGCTGCTCAACCAGAAACTG 2382 0 2092 .......................................................................................... 2181 0 1340 ........... 1330 2 473 .......................................................................................... 384 N P T T L E N E S D K Q K L M V L L R T F F E V H K G W H I Query_1 2383 AACCCAACGACGCTGGAAAACGAATCCGACAAACAGAAACTGATGGTTCTGCTACGGACGTTCTTTGAGGTGCATAAAGGCTGGCATATT 2472 0 2182 .......................................................................................... 2271 2 383 .........................T................................................................ 294 Q Y N I V S R E T L L D A K K H P D Q Y R D L V V R V A G Y Query_1 2473 CAGTACAACATCGTGTCACGCGAAACGCTGTTGGATGCGAAAAAACATCCAGACCAGTACCGCGATCTGGTCGTGCGTGTGGCAGGCTAC 2562 0 2272 .......................................................................................... 2361 2 293 .......................................................................................... 204 S A F F T A L S P D A Q D D I I A R T E H M L * H W K W I T Query_1 2563 TCCGCCTTCTTCACCGCGCTGTCGCCGGATGCGCAGGACGATATCATCGCCCGTACCGAACATATGCTGTAACATTGGAAGTGGATAACG 2652 0 2362 ..................................................................... 2430 2 203 .......................................................................................... 114 D P N S S S V D K L A A A L E H H H H H H * D P A A N K A Query_1 2653 GATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCC-G 2741 2 113 ........................................................................................N. 24 R Query_1 2742 AAA 2744 2 23 ... 21 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12018770 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 4,316 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96783.102 (IDP96783.24-134.P(134)..pMCSG53)
NDHKILGVIAMPRNETNDLTLKIPVCRIVKRIQLTADHGDIELSGASVYFKTARSASQSLNVPSSIKEGQTTGWININSDNDNKRCVSKITFSGHTVNSSDMARLKVIGDD
AACGACCATAAAATACTGGGTGTTATTGCCATGCCCAGAAATGAAACGAACGATCTCACGCTGAAAATTCCCGTCTGCCGCATCGTGAAGCGCATTCAACTCACGGCGGATCACGGCGACATCGAACTTAGCGGCGCATCCGTCTATTTTAAAACTGCGCGTAGCGCCAGCCAAAGCCTGAACGTCCCTTCCTCAATCAAAGAGGGCCAGACAACAGGCTGGATCAATATTAATAGCGATAACGACAACAAACGCTGCGTATCAAAGATTACCTTCTCAGGCCATACGGTAAATTCTTCCGATATGGCGAGACTTAAAGTCATTGGCGACGAT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,810 total letters Query= IDP96783.102 Length=738 Score E Sequences producing significant alignments: (Bits) Value 1_06C_C6_IDP96783.102_T7S.seq 1045 0.0 2_12C_C6_IDP96783.102_T7term.seq 993 0.0 0_wt 601 1e-175 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAA 90 1 12 ................N-.................. 46 2 614 .......................................................................................... 525 2 547 ...G...........T.T.C.C.C...........A... 585 E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E N Query_1 91 GAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAAC 180 1 47 ....................................------------------------------------------------...... 88 2 524 ....................................------------------------------------------------...... 483 L Y F Q S N A N D H K I L G V I A M P R N E T N D L T L K I Query_1 181 CTGTACTTCCAATCCAATGCCAACGACCATAAAATACTGGGTGTTATTGCCATGCCCAGAAATGAAACGAACGATCTCACGCTGAAAATT 270 1 89 .......................................................................................... 178 2 482 .......................................................................................... 393 0 1 ..................................................................... 69 P V C R I V K R I Q L T A D H G D I E L S G A S V Y F K T A Query_1 271 CCCGTCTGCCGCATCGTGAAGCGCATTCAACTCACGGCGGATCACGGCGACATCGAACTTAGCGGCGCATCCGTCTATTTTAAAACTGCG 360 1 179 .......................................................................................... 268 1 687 ........... 697 2 392 .......................................................................................... 303 0 70 .......................................................................................... 159 R S A S Q S L N V P S S I K E G Q T T G W I N I N S D N D N Query_1 361 CGTAGCGCCAGCCAAAGCCTGAACGTCCCTTCCTCAATCAAAGAGGGCCAGACAACAGGCTGGATCAATATTAATAGCGATAACGACAAC 450 1 269 .......................................................................................... 358 2 302 .......................................................................................... 213 0 160 .......................................................................................... 249 K R C V S K I T F S G H T V N S S D M A R L K V I G D D * H Query_1 451 AAACGCTGCGTATCAAAGATTACCTTCTCAGGCCATACGGTAAATTCTTCCGATATGGCGAGACTTAAAGTCATTGGCGACGATTAACAT 540 1 359 .......................................................................................... 448 2 212 .......................................................................................... 123 0 250 .................................................................................... 333 W K W I T D P N S S S V D K L A A A L E H H H H H H * D P A Query_1 541 TGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCT 630 1 449 .......................................................................................... 538 2 122 .......................................................................................... 33 A N K A R K E A E L A A A T A E Q * L A * P L G A S K R V L Query_1 631 GCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTG 720 1 539 .......................................................................................... 628 2 32 .................-..... 11 R G F L L K Query_1 721 AGGGGTTTTTTGCTGAAA 738 1 629 .................. 646 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1999095 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 2,810 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96786.102 (IDP96786.1-810.F..pMCSG53)
MTQLKLDTLSDRIKAHKTALVHIVKPPVCTERAQHYTEMYQQHLDKPIPVRRALALAHHLAERTIWIKHDELIVGNQASEVRAAPIFPEYTVSWIEKEIDDLADRPGAGFSVSEENKRILHDVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMTSGDAHLAVNFPLLLEKGLDGLRDKVAERRSRINLTVLEDLHGEQFLKAIDIVLDAVSQHITRFAALARQMAGEKSRESRRKELLTIAENCEVIAHEPPQTFWQALQLCYFIQLILQIESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSHSKASAGSPLYQNVTIGGQNLINGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGMSNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKWGYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMAAWDTQIRYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGIANLGNSLAAVKKLVFEQGVIGQQQLAAALANDFDGLTHEQLRQRLINGAPKYGNDDDSVDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAATMATPDGRKAHTPLAEGASPASGTDHLGPTAVIGSVGKLPTGSILGGVLLNQKLNPTTLENESDKQKLMVLLRTFFEVHKGWHIQYNIVSRETLLDAKKHPDQYRDLVVRVAGYSAFFTALSPDAQDDIIARTENML
ATGACCCAACTGAAACTGGACACGCTCAGTGACCGCATTAAGGCGCACAAAACCGCGCTGGTACATATCGTGAAACCGCCGGTCTGTACCGAGCGCGCGCAACATTATACCGAAATGTATCAGCAACATCTGGATAAACCGATTCCGGTACGCCGCGCGCTGGCGCTGGCCCATCACCTGGCGGAACGCACTATCTGGATAAAACACGATGAACTGATCGTCGGCAACCAGGCAAGTGAAGTCCGGGCCGCGCCGATTTTCCCAGAATATACCGTCTCGTGGATTGAAAAAGAGATCGACGACCTGGCAGACCGGCCCGGCGCAGGTTTTTCCGTAAGTGAAGAAAACAAACGTATTCTGCACGACGTCTGTCCGTGGTGGCGCGGTCAAACCGTCCAGGATCGCTGCTACGGCATGTTTACCGATGAACAAAAAGGGCTTCTGGCGACTGGCATTATCAAAGCCGAAGGCAATATGACTTCCGGCGATGCGCACCTGGCGGTGAACTTCCCGCTGCTGCTGGAAAAAGGGCTTGATGGCCTGCGCGATAAAGTCGCCGAGCGCCGCTCACGCATCAACCTGACCGTGCTGGAAGATCTGCATGGCGAGCAGTTCCTGAAGGCGATTGATATAGTGCTGGACGCGGTAAGCCAGCACATCACGCGCTTTGCCGCGCTGGCGCGCCAGATGGCGGGCGAAAAGTCCCGTGAAAGCCGTCGTAAAGAGCTACTCACCATCGCGGAAAACTGCGAGGTGATCGCTCACGAACCGCCGCAGACCTTCTGGCAAGCACTGCAATTGTGCTACTTCATCCAACTGATTCTACAAATTGAGTCTAACGGTCACTCGGTGTCATTTGGTCGTATGGACCAGTATCTTTACCCCTATTATCGTCGCGATGTCGAACTAAACCAGACGCTGGATCGTGAGCACGCCATTGAACTGCTGCACAGCTGCTGGCTGAAGCTGCTGGAAGTAAACAAGATCCGTTCCGGTTCGCACTCTAAAGCTTCCGCGGGCAGCCCGCTGTATCAAAACGTCACCATTGGCGGTCAGAATCTCATCAACGGTCAGCCAATGGACGCAGTGAATCCACTGTCTTACGCCATTCTGGAGTCCTGCGGACGTCTGCGTTCTACCCAGCCCAACCTCAGTGTGCGTTATCACGCCGGAATGAGTAACGATTTCCTTGACGCCTGCGTCCAGGTCATCCGCTGCGGCTTTGGGATGCCGGCGTTTAACAATGATGAAATCGTCATCCCGGAATTTATCAAGTTGGGGATCGAACCGCAGGACGCTTACGATTACGCGGCGATCGGCTGTATCGAAACCGCCGTCGGCGGGAAATGGGGTTATCGCTGCACCGGCATGAGCTTTATTAACTTCGCCCGCGTCATGCTGGCGGCGCTGGAAGGCGGTCGCGACGCCACCAGCGGTAAGGTATTTCTGCCGCAGGAAAAGGCGCTCTCCGCAGGCAACTTTAACAATTTTGACGAAGTGATGGCCGCCTGGGATACCCAAATTCGCTACTACACGCGCAAATCGATCGAAATTGAGTATGTGGTCGACACCATGCTGGAAGAGAATGTCCACGATATTCTCTGCTCAGCGCTGGTAGACGACTGTATTGAACGCGCGAAGAGTATCAAGCAAGGCGGCGCTAAATATGACTGGGTATCCGGTCTTCAGGTCGGTATCGCCAACCTGGGTAACAGCCTCGCCGCCGTGAAAAAACTGGTCTTCGAACAGGGCGTTATCGGTCAACAGCAGCTTGCCGCCGCGCTGGCCAATGACTTTGACGGGCTGACCCATGAGCAGCTGCGCCAGCGTCTGATTAACGGCGCGCCGAAGTACGGTAACGACGATGACAGTGTCGATACCCTGCTGGCGCGCGCTTATCAGACCTATATTGATGAGCTGAAGCAATACCATAACCCGCGCTATGGTCGTGGCCCTGTGGGCGGCAACTATTACGCCGGTACGTCCTCTATCTCAGCAAACGTGCCATTTGGCGCCGCAACTATGGCTACCCCGGATGGTCGCAAAGCGCATACACCGCTGGCGGAAGGCGCAAGCCCTGCATCGGGTACGGATCATCTCGGCCCGACGGCGGTGATTGGTTCCGTAGGCAAATTACCTACCGGTTCCATTCTCGGCGGCGTGCTGCTCAATCAGAAACTGAACCCAACGACGCTGGAAAACGAATCCGACAAACAGAAACTGATGGTTCTGCTACGGACGTTCTTTGAGGTGCATAAAGGCTGGCATATTCAGTACAACATCGTGTCACGCGAAACGCTGCTGGATGCGAAAAAACATCCGGACCAGTACCGCGATCTGGTCGTGCGTGTGGCAGGCTACTCCGCCTTCTTCACCGCGCTGTCGCCGGACGCGCAGGACGATATCATCGCCCGTACCGAAAATATGCTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,900 total letters Query= IDP96786.102 Length=2835 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4383 0.0 2_11D_D5_IDP96786.102_T7term.seq 1884 0.0 1_05D_D5_IDP96786.102_T7S.seq 1792 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 58 TTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGG 147 1 20 .........N...........................................................--------------------- 88 S H P Q F E K G T E N L Y F Q S N A M T Q L K L D T L S D R Query_1 148 TCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGACCCAACTGAAACTGGACACGCTCAGTGACCGC 237 0 1 .................................... 36 1 89 ---------------------------............................................................... 151 I K A H K T A L V H I V K P P V C T E R A Q H Y T E M Y Q Q Query_1 238 ATTAAGGCGCACAAAACCGCGCTGGTACATATCGTGAAACCGCCGGTCTGTACCGAGCGCGCGCAACATTATACCGAAATGTATCAGCAA 327 0 37 .......................................................................................... 126 1 152 .......................................................................................... 241 H L D K P I P V R R A L A L A H H L A E R T I W I K H D E L Query_1 328 CATCTGGATAAACCGATTCCGGTACGCCGCGCGCTGGCGCTGGCCCATCACCTGGCGGAACGCACTATCTGGATAAAACACGATGAACTG 417 0 127 .......................................................................................... 216 0 128 ........... 138 0 193 ........... 203 1 242 .......................................................................................... 331 1 243 ........... 253 I V G N Q A S E V R A A P I F P E Y T V S W I E K E I D D L Query_1 418 ATCGTCGGCAACCAGGCAAGTGAAGTCCGGGCCGCGCCGATTTTCCCAGAATATACCGTCTCGTGGATTGAAAAAGAGATCGACGACCTG 507 0 217 .......................................................................................... 306 1 332 .......................................................................................... 421 A D R P G A G F S V S E E N K R I L H D V C P W W R G Q T V Query_1 508 GCAGACCGGCCCGGCGCAGGTTTTTCCGTAAGTGAAGAAAACAAACGTATTCTGCACGACGTCTGTCCGTGGTGGCGCGGTCAAACCGTC 597 0 307 .......................................................................................... 396 1 422 .......................................................................................... 511 Q D R C Y G M F T D E Q K G L L A T G I I K A E G N M T S G Query_1 598 CAGGATCGCTGCTACGGCATGTTTACCGATGAACAAAAAGGGCTTCTGGCGACTGGCATTATCAAAGCCGAAGGCAATATGACTTCCGGC 687 0 397 .......................................................................................... 486 1 512 .......................................................................................... 601 D A H L A V N F P L L L E K G L D G L R D K V A E R R S R I Query_1 688 GATGCGCACCTGGCGGTGAACTTCCCGCTGCTGCTGGAAAAAGGGCTTGATGGCCTGCGCGATAAAGTCGCCGAGCGCCGCTCACGCATC 777 0 487 .......................................................................................... 576 1 602 .......................................................................................... 691 1 546 ........... 556 N L T V L E D L H G E Q F L K A I D I V L D A V S Q H I T R Query_1 778 AACCTGACCGTGCTGGAAGATCTGCATGGCGAGCAGTTCCTGAAGGCGATTGATATAGTGCTGGACGCGGTAAGCCAGCACATCACGCGC 867 0 577 .......................................................................................... 666 1 692 .......................................................................................... 781 F A A L A R Q M A G E K S R E S R R K E L L T I A E N C E V Query_1 868 TTTGCCGCGCTGGCGCGCCAGATGGCGGGCGAAAAGTCCCGTGAAAGCCGTCGTAAAGAGCTACTCACCATCGCGGAAAACTGCGAGGTG 957 0 667 .......................................................................................... 756 0 687 ............ 676 0 1777 ........... 1787 1 782 ..............................A........................................................... 871 I A H E P P Q T F W Q A L Q L C Y F I Q L I L Q I E S N G H Query_1 958 ATCGCTCACGAACCGCCGCAGACCTTCTGGCAAGCACTGCAATTGTGCTACTTCATCCAACTGATTCTACAAATTGAGTCTAACGGTCAC 1047 0 757 .......................................................................................... 846 1 872 ......................................................................................-... 960 S V S F G R M D Q Y L Y P Y Y R R D V E L N Q T L D R E H Query_1 1048 TCGGTGTCATTTGGTCGTAT-GGACCAGTATC-TTTACCCCTATTATCGTCGCGATGTCGAACTAAACCAG-ACGCTGGATCGTGAGCAC 1134 0 847 ....................-...........-......................................-.................. 933 1 961 ....................G...........N......................................A.....N............ 1050 A I E L L H S C W L K L L E V N K I R S G S H S K A S A Query_1 1135 -GCCATTGAACTGC-TGCACAGCTGCTGG-CTGAAGC-TGCTGGAAGTAAACAAGA-TCCGTTCCGGTTCGCACTCTAAAGCTTCCGCGG 1219 0 934 -.............-..............-.......-..................-................................. 1018 1 1051 N.............T...N..........NN...N..T........-NN.......T.............NNNNC..T........ 1135 G S P L Y Q N V T I G G Q N L I N G Q P M D A V N P L S Y A Query_1 1220 GCAGCCCGCTGTATCAAAACGTCACCATTGGCGGTCAGAATCTCATCAACGGTCAGCCAATGGACGCAGTGAATCCACTGTCTTACGCCA 1309 0 1019 .......................................................................................... 1108 I L E S C G R L R S T Q P N L S V R Y H A G M S N D F L D A Query_1 1310 TTCTGGAGTCCTGCGGACGTCTGCGTTCTACCCAGCCCAACCTCAGTGTGCGTTATCACGCCGGAATGAGTAACGATTTCCTTGACGCCT 1399 0 1109 .......................................................................................... 1198 C V Q V I R C G F G M P A F N N D E I V I P E F I K L G I E Query_1 1400 GCGTCCAGGTCATCCGCTGCGGCTTTGGGATGCCGGCGTTTAACAATGATGAAATCGTCATCCCGGAATTTATCAAGTTGGGGATCGAAC 1489 0 1199 .......................................................................................... 1288 P Q D A Y D Y A A I G C I E T A V G G K W G Y R C T G M S F Query_1 1490 CGCAGGACGCTTACGATTACGCGGCGATCGGCTGTATCGAAACCGCCGTCGGCGGGAAATGGGGTTATCGCTGCACCGGCATGAGCTTTA 1579 0 1289 .......................................................................................... 1378 2 453 ........... 463 I N F A R V M L A A L E G G R D A T S G K V F L P Q E K Query_1 1580 TTAACTTCGC-CCGCG-TCATGCTGG-CGGCGCTGGAAGGC-GGTCGCGACGCCACCAGCGGTAAGGTAT---TTCTGCCGCAGGAAAAG 1662 0 1379 ..........-.....-.........-..............-............................---................. 1461 2 1201 .....N.....N.....ANN.A...........A..C.....N...N.AC..........A..N.ATT..NN..GN...N..... 1117 A L S A G N F N N F D E V M A A W D T Q I R Y Y T R K S Query_1 1663 GCGC-TCTCCG-CAGGCAACTTTAACAA-TTTTGACGAAGTGATGGCCGCCTGGGATA-CCCAAATTCGCTACT-ACACGCGCAAA-TCG 1746 0 1462 ....-......-................-.............................-...............-...........-... 1545 2 1116 ....N.N....N................A..........NN...............N.G...............N..N........A... 1027 I E I E Y V V D T M L E E N V H D I L C S A L V D D C I E Query_1 1747 ATCGAAATTGAGTATG-TGGTCGACACCATGCTGGAAGA-GAATGTCCACGATA-TTCTCTGCTCAGCGCTGGTAGACGACTGTATTGAA 1833 0 1546 ................-......................-..............-................................... 1632 2 1026 ........A.......G.........A............A....N.........A................................... 937 R A K S I K Q G G A K Y D W V S G L Q V G I A N L G N S L Query_1 1834 CGCGCGAAGAGTATCAAGC-AAGGCGGCGCTAAATATGACTGGGTATCCGGTCTTCA-GGTCGGTATCGCCAACCTGGGTAACAGCCTCG 1921 0 1633 ...................-.....................................-................................ 1720 2 936 ...................C.......................N.............A................................ 847 A A V K K L V F E Q G V I G Q Q Q L A A A L A N D F D G L T Query_1 1922 CCGCCGTGAAAAAACTGGTCTTCGAACAGGGCGTTATCGGTCAACAGCAGCTTGCCGCCGCGCTGGCCAATGACTTTGACGGGCTGACCC 2011 0 1721 .......................................................................................... 1810 0 670 ........... 680 2 846 .......................................................................................... 757 1 785 ........... 795 H E Q L R Q R L I N G A P K Y G N D D D S V D T L L A R A Y Query_1 2012 ATGAGCAGCTGCGCCAGCGTCTGATTAACGGCGCGCCGAAGTACGGTAACGACGATGACAGTGTCGATACCCTGCTGGCGCGCGCTTATC 2101 0 1811 .......................................................................................... 1900 2 756 .......................................................................................... 667 Q T Y I D E L K Q Y H N P R Y G R G P V G G N Y Y A G T S S Query_1 2102 AGACCTATATTGATGAGCTGAAGCAATACCATAACCCGCGCTATGGTCGTGGCCCTGTGGGCGGCAACTATTACGCCGGTACGTCCTCTA 2191 0 1901 .......................................................................................... 1990 2 666 .......................................................................................... 577 I S A N V P F G A A T M A T P D G R K A H T P L A E G A S P Query_1 2192 TCTCAGCAAACGTGCCATTTGGCGCCGCAACTATGGCTACCCCGGATGGTCGCAAAGCGCATACACCGCTGGCGGAAGGCGCAAGCCCTG 2281 0 1991 .......................................................................................... 2080 2 576 .......................................................................................... 487 A S G T D H L G P T A V I G S V G K L P T G S I L G G V L L Query_1 2282 CATCGGGTACGGATCATCTCGGCCCGACGGCGGTGATTGGTTCCGTAGGCAAATTACCTACCGGTTCCATTCTCGGCGGCGTGCTGCTCA 2371 0 2081 .......................................................................................... 2170 0 1340 ........... 1330 2 486 .......................................................................................... 397 N Q K L N P T T L E N E S D K Q K L M V L L R T F F E V H K Query_1 2372 ATCAGAAACTGAACCCAACGACGCTGGAAAACGAATCCGACAAACAGAAACTGATGGTTCTGCTACGGACGTTCTTTGAGGTGCATAAAG 2461 0 2171 .......................................................................................... 2260 2 396 .......................................................................................... 307 G W H I Q Y N I V S R E T L L D A K K H P D Q Y R D L V V R Query_1 2462 GCTGGCATATTCAGTACAACATCGTGTCACGCGAAACGCTGCTGGATGCGAAAAAACATCCGGACCAGTACCGCGATCTGGTCGTGCGTG 2551 0 2261 .......................................................................................... 2350 2 306 .......................................................................................... 217 V A G Y S A F F T A L S P D A Q D D I I A R T E N M L * H W Query_1 2552 TGGCAGGCTACTCCGCCTTCTTCACCGCGCTGTCGCCGGACGCGCAGGACGATATCATCGCCCGTACCGAAAATATGCTGTAACATTGGA 2641 0 2351 ................................................................................ 2430 2 216 .......................................................................................... 127 K W I T D P N S S S V D K L A A A L E H H H H H H * D P A A Query_1 2642 AGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCT- 2730 2 126 .........................................................................................A 37 N K A R Query_1 2731 AACAAAGCCCGAAA 2744 2 36 ..........N... 23 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 13664482 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 4,900 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96793.102 (IDP96793.1-349.F..pMCSG53)
MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRNLPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPLPHAHQQQNPQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATGVPG
GTGCGGGTATTACTTCGACCTGTTCTGGTTCCGGAACTCGGGCTGGTGGTCCTTAAGCCCGGTCGTGAATCCATGCCGGTATTCCACAATACCCGGGTACTGGTGGAGCCGGAACCGAAAAGCATGCGTAATCTGCCGTCCGGGGTCGTTCCTGCCGTTCGCCAGCCGCTAGTGGAAGACAAAACATTGCTGCCGTTTTTCAGTAACGCACGGGTGATTCGTGCTGCTGGTGGTGCTGGCGCATTGTCTGACTGGCTGTTGCGCCATATTAAATCCTGCCAGTGGCCACACGGCGATTATCATCACAGCGAAACCGTCATTCACCGTTATGGTACCGGCGCAATGGTATTGTGCTGGCACTGCGACAACCAGCTGCGTGACCAGACATCCGAATCACTCGAGCAACTTGCTCATCAAAACCTGTCAGCATGGATGATTGACGTCATCGGTCACGCAATAAGCGGTACGCAGGAGCGTGAATTATCTCTGGCTGAATTATCCTGGTGGGCGGTCCGCAATCAGGTGGCGGACGCGCTACCGGAAGCGGTATTACGTCGTTCGCTGGGGTTGCGTGCGGAAAAAATCCGCTCAATGTACCGTGAAAGCGACATCGTGCCGGGAGAGCAGACCGCCACCAGCATACTGAAGCAGCGCACAAAAAATCTTGCGCCGCTGCCTCACGCCCACCAGCAACAGAACCCACAACAGGAAAAGACGGTGGTCAGCATTGCCGTTGATCCTGAGTCTCCGGAATCTTTCATGAAACGACCTAAACGTCGCCGCTGGGTTAACGAGAAATACAAACGCTGGGTGAAGACACAGCCGTGTGCGTGTTGTGGTAAGCCAGCCGACGATCCCCATCACCTGATTGGTCATGGTCAGGGCGGAATGGGGACCAAATCTCACGATATTTTCACGCTACCGCTGTGTCGGGAGCATCACAACGAGCTTCATGCGGATCCTCTGGCGTTCGAAGAAAAGCATGGTTCTCAGGTTGATTTAATTTTTCGTTTTCTTGATCACGCCTTTGCAACTGGCGTGCCTGGG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,602 total letters Query= IDP96793.102 Length=1452 Score E Sequences producing significant alignments: (Bits) Value 2_12H_H6_IDP96793.102_T7term.seq 1952 0.0 0_wt 1889 0.0 1_06H_H6_IDP96793.102_T7S.seq 1799 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W S Query_1 61 CCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCT 150 1 19 ..................................................................------------------------ 84 H P Q F E K G T E N L Y F Q S N A V R V L L R P V L V P Query_1 151 CATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTA-CTTCCAAT-CCAATGCCGTGCGGGTATT-ACTTC-GACCTGTT-CTGGTTCCGG 235 2 1209 .....A..NA....T......AN....C......T.....N...T.N..T...N.....A 1150 0 1 ...........-.....-........-.......... 34 1 85 ------------------------...........-........-...................-.....-........-.......... 145 E L G L V V L K P G R E S M P V F H N T R V L V E P E P K S Query_1 236 AACTCGGGCTGGTGGTCCTTAAGCCCGGT-CGTGAATCCATGCCGGTATTCCACAATACCCGGGTACTGGTGGAGCCGGAACCGAAAAGC 324 2 1149 .....A..N..-....T.....N......T.....T...........NN.N....TN..........N.TG.A................. 1061 0 35 .............................-............................................................ 123 1 146 .............................-............................................................ 234 M R N L P S G V V P A V R Q P L V E D K T L L P F F S N A Query_1 325 ATGCGTAAT-CTGCCGTCCGGGGTCGTTCCT-GCCGTTCGCCAGCCGCTAGTGGAAGACAAAACATTGCTGCCGTTTTTCAGTAACGCAC 412 2 1060 .........TNNN.....N.....N......T.......................................................... 971 0 124 .........-.....................-.......................................................... 211 1 235 .........-.....................-.......................................................... 322 R V I R A A G G A G A L S D W L L R H I K S C Q W P H G D Query_1 413 GGGTGATTCGTGCTGCTGGT-GGTGCTGGCGCATTGTCTGACTGGCTGTTGCGCCATATTAAATCCTGCCAGTGGCCACACGGCGA-TTA 500 2 970 ....................T.................................................................W... 881 0 212 ....................-.................................................................-... 299 1 323 ....................-.................................................................-... 410 Y H H S E T V I H R Y G T G A M V L C W H C D N Q L R D Q T Query_1 501 TCATCACAGCGAAACCGTCATTCACCGTTATGGTACCGGCGCAATGGTATTGTGCTGGCACTGCGACAACCAGCTGCGTGACCAGACATC 590 2 880 .......................................................................................... 791 0 300 .......................................................................................... 389 1 411 .......................................................................................... 500 S E S L E Q L A H Q N L S A W M I D V I G H A I S G T Q E R Query_1 591 CGAATCACTCGAGCAACTTGCTCATCAAAACCTGTCAGCATGGATGATTGACGTCATCGGTCACGCAATAAGCGGTACGCAGGAGCGTGA 680 2 790 .......................................................................................... 701 0 390 .......................................................................................... 479 1 501 .......................................................................................... 590 E L S L A E L S W W A V R N Q V A D A L P E A V L R R S L G Query_1 681 ATTATCTCTGGCTGAATTATCCTGGTGGGCGGTCCGCAATCAGGTGGCGGACGCGCTACCGGAAGCGGTATTACGTCGTTCGCTGGGGTT 770 2 700 .......................................................................................... 611 0 480 .......................................................................................... 569 1 591 .......................................................................................... 680 L R A E K I R S M Y R E S D I V P G E Q T A T S I L K Q R T Query_1 771 GCGTGCGGAAAAAATCCGCTCAATGTACCGTGAAAGCGACATCGTGCCGGGAGAGCAGACCGCCACCAGCATACTGAAGCAGCGCACAAA 860 2 610 .......................................................................................... 521 0 570 .......................................................................................... 659 1 681 .......................................................................................... 770 K N L A P L P H A H Q Q Q N P Q Q E K T V V S I A V D P E S Query_1 861 AAATCTTGCGCCGCTGCCTCACGCCCACCAGCAACAGAA-CCCACAACAGGAAAAGACGGTGGTCAGCATTGCCGTTGATCCTGAGTCTC 949 2 520 .......................................-.................................................. 432 0 660 .......................................-.................................................. 748 1 771 .......................................M.................................................. 860 P E S F M K R P K R R R W V N E K Y K R W V K T Q P C A C Query_1 950 CGGAATC-TTTCATGAAACGACCTAAACGTCGCCGCTGGGTTAACGAGAAATACAAACGCTGGGTGAAGACACAGCCGTG-TGCGTGTTG 1037 2 431 .......-........................................................................-......... 344 0 749 .......-........................................................................-......... 836 1 861 .......T........................................................................T......... 950 C G K P A D D P H H L I G H G Q G G M G T K S H D I F T L Query_1 1038 TGGTAAGCCAGCCGACGATCCCCATCA-CCTGATTGGTCA-TGGT-CAGGGCGG-AATGGGGACCAAATCTCACGATATTTTCACGCTA- 1122 2 343 ...........................-............-....-........-..................................- 259 0 837 ...........................-............-....-........-..................................- 921 1 951 ......................NT...N......N.....N....N........A..........................N.......C 1040 P L C R E H H N E L H A D P L A F E E K H G S Q V D Query_1 1123 CCGCTGTGTCGGGAGC-ATCACAACG-AGCTTCAT-GCGG-ATCCTCTGGCGTT-CGAA--GAAAAGCATGG-TTCT-CAGGTTG-ATTT 1202 2 258 ................-.........-........-....-.............-....--...........-....-.......-.... 179 0 922 ................-.........-........-....-.............-....--...........-....-.......-.... 1001 1 1041 ................N.........A........G....A.............N....AGA....N.....N....NN...N..A.... 1130 L I F R F L D H A F A T G V P G * H W K W I T D P N S S S Query_1 1203 AATTTTTCGTTTTCTT-GATCACGCCTTT-GCAACTGGCGTGCCTGGGTAACATTGGAAGTG-GATAACGG-ATCCGAATTCGAGCTC-C 1287 2 178 ................-............-................................-........-................-. 94 0 1002 ................-............-.................. 1047 1 1131 ................G............N........GCGNN.N....T..CA....N...N.N.N....N..N.-N.N..NNNN..T. 1219 V D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 1288 GTCGACAAG-CTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAG 1366 2 93 .........-..................................................................A... 15 1 1220 .........T.... 1233 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5103544 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 3,602 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96793.103 (IDP96793.50-349.P(349)..pMCSG53)
VPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPLPHAHQQQNPQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATGVPG
GTTCCTGCCGTTCGCCAGCCGCTAGTGGAAGACAAAACATTGCTGCCGTTTTTCAGTAACGCACGGGTGATTCGTGCTGCTGGTGGTGCTGGCGCATTGTCTGACTGGCTGTTGCGCCATATTAAATCCTGCCAGTGGCCACACGGCGATTATCATCACAGCGAAACCGTCATTCACCGTTATGGTACCGGCGCAATGGTATTGTGCTGGCACTGCGACAACCAGCTGCGTGACCAGACATCCGAATCACTCGAGCAACTTGCTCATCAAAACCTGTCAGCATGGATGATTGACGTCATCGGTCACGCAATAAGCGGTACGCAGGAGCGTGAATTATCTCTGGCTGAATTATCCTGGTGGGCGGTCCGCAATCAGGTGGCGGACGCGCTACCGGAAGCGGTATTACGTCGTTCGCTGGGGTTGCGTGCGGAAAAAATCCGCTCAATGTACCGTGAAAGCGACATCGTGCCGGGAGAGCAGACCGCCACCAGCATACTGAAGCAGCGCACAAAAAATCTTGCGCCGCTGCCTCACGCCCACCAGCAACAGAACCCACAACAGGAAAAGACGGTGGTCAGCATTGCCGTTGATCCTGAGTCTCCGGAATCTTTCATGAAACGACCTAAACGTCGCCGCTGGGTTAACGAGAAATACAAACGCTGGGTGAAGACACAGCCGTGTGCGTGTTGTGGTAAGCCAGCCGACGATCCCCATCACCTGATTGGTCATGGTCAGGGCGGAATGGGGACCAAATCTCACGATATTTTCACGCTACCGCTGTGTCGGGAGCATCACAACGAGCTTCATGCGGATCCTCTGGCGTTCGAAGAAAAGCATGGTTCTCAGGTTGATTTAATTTTTCGTTTTCTTGATCACGCCTTTGCAACTGGCGTGCCTGGG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,379 total letters Query= IDP96793.103 Length=1305 Score E Sequences producing significant alignments: (Bits) Value 2_10B_B7_IDP96793.103_T7term.seq 1873 0.0 1_01C_B7_IDP96793.103_T7short.seq 1806 0.0 0_wt 1624 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAA 90 2 1166 ......NN.......N.--..........N.NN.............N......N..--.......................--....--. 1085 2 1103 ...G......--..NT.T.C.N.C........... 1135 1 4 ..T.............-................... 38 E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E N Query_1 91 GAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAAC 180 2 1084 ...........N.........-..............------------------------------------------------...... 1044 1 39 ....................................------------------------------------------------...... 80 L Y F Q S N A V P A V R Q P L V E D K T L L P F F S N A R V Query_1 181 CTGTACTTCCAATCCAATGCCGTTCCTGCCGTTCGCCAGCCGCTAGTGGAAGACAAAACATTGCTGCCGTTTTTCAGTAACGCACGGGTG 270 2 1043 .......C.--............-.....................AGT.............-............................ 958 1 81 .......................................................................................... 170 0 1 ..................................................................... 69 I R A A G G A G A L S D W L L R H I K S C Q W P H G D Y H H Query_1 271 ATTCGTGCTGCTGGTGGTGCTGGCGCATTGTCTGACTGGCTGTTGCGCCATATTAAATCCTGCCAGTGGCCACACGGCGATTATCATCAC 360 2 957 .................................................................................-........ 869 1 171 .......................................................................................... 260 0 70 .......................................................................................... 159 S E T V I H R Y G T G A M V L C W H C D N Q L R D Q T S E S Query_1 361 AGCGAAACCGTCATTCACCGTTATGGTACCGGCGCAATGGTATTGTGCTGGCACTGCGACAACCAGCTGCGTGACCAGACATCCGAATCA 450 2 868 .......................................................................................... 779 1 261 .......................................................................................... 350 0 160 .......................................................................................... 249 L E Q L A H Q N L S A W M I D V I G H A I S G T Q E R E L S Query_1 451 CTCGAGCAACTTGCTCATCAAAACCTGTCAGCATGGATGATTGACGTCATCGGTCACGCAATAAGCGGTACGCAGGAGCGTGAATTATCT 540 2 778 .......................................................................................... 689 1 351 .......................................................................................... 440 0 250 .......................................................................................... 339 L A E L S W W A V R N Q V A D A L P E A V L R R S L G L R A Query_1 541 CTGGCTGAATTATCCTGGTGGGCGGTCCGCAATCAGGTGGCGGACGCGCTACCGGAAGCGGTATTACGTCGTTCGCTGGGGTTGCGTGCG 630 2 688 .......................................................................................... 599 1 441 .......................................................................................... 530 0 340 .......................................................................................... 429 E K I R S M Y R E S D I V P G E Q T A T S I L K Q R T K N L Query_1 631 GAAAAAATCCGCTCAATGTACCGTGAAAGCGACATCGTGCCGGGAGAGCAGACCGCCACCAGCATACTGAAGCAGCGCACAAAAAATCTT 720 2 598 .......................................................................................... 509 1 531 .......................................................................................... 620 0 430 .......................................................................................... 519 A P L P H A H Q Q Q N P Q Q E K T V V S I A V D P E S P E S Query_1 721 GCGCCGCTGCCTCACGCCCACCAGCAACAGAACCCACAACAGGAAAAGACGGTGGTCAGCATTGCCGTTGATCCTGAGTCTCCGGAATCT 810 2 508 .......................................................................................... 419 1 621 .......................................................................................... 710 0 520 .......................................................................................... 609 F M K R P K R R R W V N E K Y K R W V K T Q P C A C C G K P Query_1 811 TTCATGAAACGACCTAAACGTCGCCGCTGGGTTAACGAGAAATACAAACGCTGGGTGAAGACACAGCCGTGTGCGTGTTGTGGTAAGCCA 900 2 418 .......................................................................................... 329 1 711 .......................................................................................... 800 0 610 .......................................................................................... 699 A D D P H H L I G H G Q G G M G T K S H D I F T L P L C R E Query_1 901 GCCGACGATCCCCATCACCTGATTGGTCATGGTCAGGGCGGAATGGGGACCAAATCTCACGATATTTTCACGCTACCGCTGTGTCGGGAG 990 2 328 .......................................................................................... 239 1 801 .......................................................................................... 890 0 700 .......................................................................................... 789 H H N E L H A D P L A F E E K H G S Q V D L I F R F L D H A Query_1 991 CATCACAACGAGCTTCATGCGGATCCTCTGGCGTTCGAAGAAAAGCATGGTTCTCAGGTTGATTTAATTTTTCGTTTTCTTGATCACGCC 1080 2 238 .......................................................................................... 149 1 891 ........................................-................................................. 979 0 790 .......................................................................................... 879 F A T G V P G * H W K W I T D P N S S S V D K L A A A L E H Query_1 1081 TTTGCAACTGGCGTGCCTGGGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcac 1170 2 148 .......................................................................................... 59 1 980 ...................................--NT..................CT.......-.........N............. 1066 0 880 ..................... 900 H H H H H * D P A A N K A R K E A E L A A Query_1 1171 cacca-ccaccaccacTGAGAT-CCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG 1234 2 58 .....-................-........................ 14 1 1067 .....C...T..-.N.......N.........-.NN...N..NNNG...NCTG..N.......... 1130 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4293659 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 3,379 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96796.102 (IDP96796.1-162.F..pMCSG53)
MTDMNPDIEKDQTSDEVTVETTSVFRADFLSELDAPAQAGTESAVSGVEGLPPGSALLVVKRGPNAGSRFLLDQAITSAGRHPDSDIFLDDVTVSRRHAEFRLENNEFNVVDVGSLNGTYVNREPVDSAVLANGDEVQIGKFRLVFLTGPKQGEDDGSTGGP
GTGACGGACATGAACCCGGATATTGAGAAGGACCAGACCTCCGATGAAGTCACGGTAGAGACGACCTCCGTCTTCCGCGCAGACTTCCTCAGCGAGCTGGACGCTCCTGCGCAAGCGGGTACGGAGAGCGCGGTCTCCGGGGTGGAAGGGCTCCCGCCGGGCTCGGCGTTGCTGGTAGTCAAACGAGGCCCCAACGCCGGGTCCCGGTTCCTACTCGACCAAGCCATCACGTCGGCTGGTCGGCATCCCGACAGCGACATATTTCTCGACGACGTGACCGTGAGCCGTCGCCATGCTGAATTCCGGTTGGAAAACAACGAATTCAATGTCGTCGATGTCGGGAGTCTCAACGGCACCTACGTCAACCGCGAGCCCGTGGATTCGGCGGTGCTGGCGAACGGCGACGAGGTCCAGATCGGCAAGTTCCGGTTGGTGTTCTTGACCGGACCCAAGCAAGGCGAGGATGACGGGAGTACCGGGGGCCCG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,987 total letters Query= IDP96796.102 Length=891 Score E Sequences producing significant alignments: (Bits) Value 1_03A_A3_IDP96796.102_T7S.seq 1315 0.0 2_09A_A3_IDP96796.102_T7term.seq 1234 0.0 0_wt 877 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L Query_1 1 TCTCGATCCCGCGAAA-TTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAA-TAATTTTGTTTAACTTT 88 1 22 ...........--................. 49 2 779 ................A.......................................................A................. 690 2 711 ...G...........T.T.C.C.C...........A... 749 * E G D I H M H H H H H H S S G V D L W S H P Q F E K G T Query_1 89 AAG--AAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGA 176 1 50 ...--...................................------------------------------------------------.. 89 2 689 ..AGA...................................------------------------------------------------.. 648 E N L Y F Q S N A V T D M N P D I E K D Q T S D E V T V E T Query_1 177 GAACCTGTACTTCCAATCCAATGCCGTGACGGACATGAACCCGGATATTGAGAAGGACCAGACCTCCGATGAAGTCACGGTAGAGACGAC 266 1 90 .......................................................................................... 179 2 647 .......................................................................................... 558 0 1 ................................................................. 65 T S V F R A D F L S E L D A P A Q A G T E S A V S G V E G L Query_1 267 CTCCGTCTTCCGCGCAGACTTCCTCAGCGAGCTGGACGCTCCTGCGCAAGCGGGTACGGAGAGCGCGGTCTCCGGGGTGGAAGGGCTCCC 356 1 180 .......................................................................................... 269 2 557 .......................................................................................... 468 0 66 .......................................................................................... 155 P P G S A L L V V K R G P N A G S R F L L D Q A I T S A G R Query_1 357 GCCGGGCTCGGCGTTGCTGGTAGTCAAACGAGGCCCCAACGCCGGGTCCCGGTTCCTACTCGACCAAGCCATCACGTCGGCTGGTCGGCA 446 1 270 .......................................................................................... 359 2 467 .......................................................................................... 378 0 156 .......................................................................................... 245 H P D S D I F L D D V T V S R R H A E F R L E N N E F N V V Query_1 447 TCCCGACAGCGACATATTTCTCGACGACGTGACCGTGAGCCGTCGCCATGCTGAATTCCGGTTGGAAAACAACGAATTCAATGTCGTCGA 536 1 360 .......................................................................................... 449 2 377 .......................................................................................... 288 0 246 .......................................................................................... 335 D V G S L N G T Y V N R E P V D S A V L A N G D E V Q I G K Query_1 537 TGTCGGGAGTCTCAACGGCACCTACGTCAACCGCGAGCCCGTGGATTCGGCGGTGCTGGCGAACGGCGACGAGGTCCAGATCGGCAAGTT 626 1 450 .......................................................................................... 539 2 287 .......................................................................................... 198 0 336 .......................................................................................... 425 F R L V F L T G P K Q G E D D G S T G G P * H W K W I T D P Query_1 627 CCGGTTGGTGTTCTTGACCGGACCCAAGCAAGGCGAGGATGACGGGAGTACCGGGGGCCCGTAACATTGGAAGTGGATAACGGATCCGAA 716 1 540 .......................................................................................... 629 2 197 .......................................................................................... 108 0 426 ............................................................. 486 N S S S V D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 717 TTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAAC-AAAGCCCGAAAGGAAG 805 1 630 .........................................................................-................ 718 2 107 .........................................................................A........... 23 A E L A A A T A E Q * L A * P L G A S K R V L R G F L L K Query_1 806 CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAA 891 1 719 ...................................................................................... 804 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2577192 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 2,987 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96798.102 (IDP96798.1-322.F..pMCSG53)
MKKETKPRVVVIGGGTGLPVILKGLKKKDIHLTAIVTVADDGGSSGKIREQMDVLPPGDIRNVMLALSNVDPRVVDLFQYRFAVDGDLSGHVIGNLILTALSQLNDSYVDAINVLATVLKIRGKVIPATDQPLILNAEMEDGSIVHGESLIPLQGKHINRVYIEPENVKPYPTAVEAVKEADLIVIGPGSLYTSILPNLLLTELADEITASKAPKVYITNILTQIGETDFFSDADHIKVIHEHVGKSFIDKTLINTTTVPKELLFPEDVAQVEHNAKGMEELGVEAIYQDFLSTEDGLVRHAAEKVADALLAMLPNQTNEKE
ATGAAAAAGGAAACGAAACCTAGAGTAGTAGTAATCGGTGGGGGGACTGGTCTTCCAGTTATTTTAAAAGGGCTTAAAAAGAAGGACATTCATCTAACGGCCATCGTGACAGTAGCGGACGATGGTGGTAGTTCTGGTAAAATTCGCGAGCAAATGGATGTGCTTCCACCAGGTGATATACGCAATGTTATGCTGGCTTTATCCAATGTCGATCCACGTGTTGTTGATTTATTCCAGTATCGTTTTGCGGTAGATGGTGATTTATCGGGACATGTTATTGGGAATTTGATTTTAACGGCTTTATCGCAATTGAATGATAGTTATGTTGATGCGATTAATGTGCTAGCAACGGTGCTGAAAATCCGCGGCAAAGTTATTCCAGCAACGGATCAACCACTTATTTTAAATGCCGAAATGGAAGATGGTTCTATTGTGCACGGTGAATCGCTTATCCCGTTACAAGGAAAGCATATTAATCGTGTGTATATCGAACCAGAAAATGTAAAACCTTATCCGACAGCAGTCGAAGCAGTGAAAGAGGCTGATTTGATTGTTATTGGTCCAGGAAGTTTATACACAAGTATCTTACCAAATCTATTACTTACGGAGTTGGCCGATGAAATCACAGCAAGTAAGGCTCCGAAAGTTTATATTACGAATATTTTAACGCAAATTGGCGAAACCGACTTTTTCTCAGATGCGGATCACATTAAAGTTATCCATGAGCATGTGGGGAAATCATTTATAGATAAAACGTTAATTAATACGACGACTGTTCCAAAAGAGCTTCTATTCCCAGAAGACGTTGCGCAAGTAGAGCATAATGCTAAAGGAATGGAAGAACTTGGTGTAGAAGCAATTTATCAAGATTTTCTTTCTACAGAGGATGGTCTTGTGCGCCATGCCGCTGAAAAGGTGGCGGACGCGCTTTTAGCGATGTTACCTAACCAAACTAATGAAAAGGAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,521 total letters Query= IDP96798.102 Length=1371 Score E Sequences producing significant alignments: (Bits) Value 2_08G_G2_IDP96798.102_T7term.seq 1894 0.0 1_02G_G2_IDP96798.102_T7S.seq 1842 0.0 0_wt 1743 0.0 I N T T H Y R G I V S G * Q F P S R N N F V * L * E G D I Query_1 15 AATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCC-TCTAGAAA-TAATTTTGTTTAACTTTAAGAAGGAGATATA 102 2 1199 ......AC.N....T-.NN.....-..NN.--.G..................NNN- 1149 2 339 ............ 328 1 17 ........CN.......A............................... 65 1 876 ............ 887 0 759 ............ 770 H M H H H H H H S S G V D L W S H P Q F E K G T E N L Y F Q Query_1 103 CATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAA 192 2 1115 ....N...N 1107 2 1148 .N...................... 1125 1 66 ........................------------------------------------------------.................. 107 S N A M K K E T K P R V V V I G G G T G L P V I L K G L K Query_1 193 TCCAATGCCATGAAAAAGGAAACGAAACCTAGAGTAGTAGTAAT-CGGTGG-GGGGACTGGTCTTCCAGTT-ATTTTAAAAGGGCTTAAA 279 2 1106 N.-......N......G.--......-.................T......A...................T.....T............ 1021 1 108 ............................................-......-...................-.................. 194 0 1 ...................................-......-...................-.................. 78 K K D I H L T A I V T V A D D G G S S G K I R E Q M D V L P Query_1 280 AAGAAGGACATTCATCTAACGGCCATCGTGACAGTAGCGGACGATGGTGGTAGTTCTGGTAAAATTCGCGAGCAAATGGATGTGCTTCCA 369 2 1020 .......................................................................................... 931 1 195 .......................................................................................... 284 0 79 .......................................................................................... 168 P G D I R N V M L A L S N V D P R V V D L F Q Y R F A V D Query_1 370 CCAGGTGATATACGCAATGTTATGCTGGCTTTATCCAATGTCGATCCACGTGTTGTTGATTT-ATTCCAGTATCGTTTTGCGGTAGATGG 458 2 930 ..............................................................T........................... 841 1 285 ..............................................................-........................... 373 0 169 ..............................................................-........................... 257 G D L S G H V I G N L I L T A L S Q L N D S Y V D A I N V L Query_1 459 TGATTTATCGGGACATGTTATTGGGAATTTGATTTTAACGGCTTTATCGCAATTGAATGATAGTTATGTTGATGCGATTAATGTGCTAGC 548 2 840 .......................................................................................... 751 1 374 .......................................................................................... 463 0 258 .......................................................................................... 347 A T V L K I R G K V I P A T D Q P L I L N A E M E D G S I V Query_1 549 AACGGTGCTGAAAATCCGCGGCAAAGTTATTCCAGCAACGGATCAACCACTTATTTTAAATGCCGAAATGGAAGATGGTTCTATTGTGCA 638 2 750 .......................................................................................... 661 1 464 .......................................................................................... 553 0 348 .......................................................................................... 437 H G E S L I P L Q G K H I N R V Y I E P E N V K P Y P T A V Query_1 639 CGGTGAATCGCTTATCCCGTTACAAGGAAAGCATATTAATCGTGTGTATATCGAACCAGAAAATGTAAAACCTTATCCGACAGCAGTCGA 728 2 660 .......................................................................................... 571 1 554 .......................................................................................... 643 0 438 .......................................................................................... 527 E A V K E A D L I V I G P G S L Y T S I L P N L L L T E L A Query_1 729 AGCAGTGAAAGAGGCTGATTTGATTGTTATTGGTCCAGGAAGTTTATACACAAGTATCTTACCAAATCTATTACTTACGGAGTTGGCCGA 818 2 570 .......................................................................................... 481 1 644 .......................................................................................... 733 0 528 .......................................................................................... 617 D E I T A S K A P K V Y I T N I L T Q I G E T D F F S D A D Query_1 819 TGAAATCACAGCAAGT-AAGGCTCCGAAAGTTTATATTACGAATATTTTAACGCAAATTGGCGAAACCGACTTTTTCTCAGATGCGGATC 907 2 480 ................-......................................................................... 392 1 734 ................A......................................................................... 823 0 618 ................-......................................................................... 706 H I K V I H E H V G K S F I D K T L I N T T T V P K E L L F Query_1 908 ACATTAAAGTTATCCATGAGCATGTGGGGAAATCATTTATAGATAAAACGTTAATTAATACGACGACTGTTCCAAAAGAGCTTCTATTCC 997 2 391 .......................................................................................... 302 1 824 .......................................................................................... 913 0 707 .......................................................................................... 796 P E D V A Q V E H N A K G M E E L G V E A I Y Q D F L S T E Query_1 998 CAGAAGACGTTGCGCAAGTAGAGCATAATGCTAAAGGAATGGAAGAACTTGGTGTAGAAGCAATTTATCAAGATTTTC-TTTCTACAGAG 1086 2 301 ..............................................................................-........... 213 1 914 ..............................................................................T........... 1003 0 797 ..............................................................................-........... 885 D G L V R H A A E K V A D A L L A M L P N Q T N E K E * H Query_1 1087 GATGGTCTTGTGCGCCATGCCGCTGAAAAGGTGGCGGACGCGCTTTTAGCGATGTTACCTAACCAAA-CTAATGAAAAGGAGTAACATTG 1175 2 212 ...................................................................-...................... 124 1 1004 .................................NN................................C...N...........-....G. 1092 0 886 ...................................................................-.............. 966 W K W I T D P N S S S V D K L A A A L E H H H H H H * Query_1 1176 GAAGTGGATAACGGATCCG-AATTCGA--GCTCC-GTCGA-CAAGCTTGCGGCCGCACT--CGAGcacc-accaccaccaccacT-GAGA 1256 2 123 ........-..........-.......--.....-.....-..................--........A...............-.... 43 1 1093 C.......-..........A.....N.AGC....G.....C..G............NN.TCNNN...N.-NA...--A...A...G..N. 1178 D P A A N K A R K E A E L A Query_1 1257 -TCCGGCT-GCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCT 1296 2 42 -.......-..................A.... 13 1 1179 T.......G.................-.NN....NNN..... 1219 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4705915 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 3,521 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96801.102 (IDP96801.1-655.F..pMCSG53)
MMIGKRLSDRYKILHAIGGGGMANVYLAHDIILDRDVAVKILRIDLADESNLIRRFQREAQSATSLVHPNIVSVYDVGEENDLHYIVMEHVDGMDLKQYIHENHPISYEKAVDIMLQIVSAVAIAHQHHIIHRDLKPQNILIDHDGVVKITDFGIAMALSETSITQTNSLLGSVHYLSPEQARGGMATQKSDIYSLGIVLYELLTGKVPFDGESAVSIAIKHLQADIPSAREQNPEIPQSLENIIIKATAKDPFLRYQNAEEMEKDLQTCLNKDRLNEPKYVFPTDDGDTKAIPIIATKDTMQNLDKTIVPEGKVAAAEVVPEEKKDKKKKKMSKKKKIALIVSSVIIIFIIGILLLWLLGKSPDEVAVPDVSGKTEDQAVALLQKEGFVIGKTAEKNSDEVDEGKVINTDPEAGEMKEKGTKINLFVSIGSKKITMDDYTGRSYTDTKALLEEQGFKNISSEEAYSSEIEKGLIISQTPTQGTEVVAKSTDVKFVVSKGAEPITLKDLRGYTKTAVEDYASPLGLKVSSKEENSSTVEKGQVISQSPSAGTAMNSGDTIEIVISAGPKEKQVKEVTKTFNIPYTPSDEENPQPQKIQIYIQDKDHSMTSAYREMSITQNTSVEITFQIEEGSSAGYKIISDDKVIDEGTVPYPN
ATGATGATTGGTAAGCGATTAAGCGATCGATATAAGATTTTACATGCAATAGGCGGCGGCGGAATGGCCAATGTGTACTTGGCGCATGATATTATTCTTGATAGAGATGTTGCAGTGAAAATTTTACGAATCGATTTGGCAGATGAAAGCAACCTTATTCGTCGGTTCCAACGAGAAGCTCAATCAGCAACAAGTCTCGTTCACCCTAATATTGTTAGTGTGTACGATGTTGGTGAAGAAAATGATTTACACTATATTGTAATGGAACATGTAGATGGGATGGATTTAAAACAGTACATTCACGAAAATCATCCAATCAGTTATGAAAAAGCAGTAGATATTATGCTGCAAATCGTTTCTGCGGTAGCAATCGCCCATCAACATCACATTATCCACCGTGACTTAAAACCGCAAAATATTTTAATTGATCATGATGGTGTCGTGAAGATTACAGATTTTGGTATTGCAATGGCACTTTCAGAAACATCTATTACACAAACGAATTCTTTACTAGGTTCGGTGCATTACTTATCCCCAGAACAAGCACGTGGCGGAATGGCAACACAAAAATCAGATATCTATTCACTTGGCATTGTGTTATACGAACTATTAACTGGAAAAGTACCTTTTGACGGGGAGTCAGCAGTTTCTATAGCTATTAAGCATCTGCAAGCAGACATCCCATCCGCAAGAGAACAAAATCCAGAAATTCCACAAAGCTTAGAAAATATTATCATTAAAGCAACGGCGAAAGATCCATTTTTACGTTACCAAAACGCGGAAGAAATGGAAAAAGACTTGCAAACTTGTTTAAATAAAGACCGTTTGAACGAGCCAAAATATGTTTTCCCAACAGATGATGGTGATACAAAAGCAATTCCAATCATCGCAACAAAAGATACGATGCAAAACCTTGATAAAACGATTGTTCCAGAAGGAAAAGTAGCCGCTGCCGAAGTAGTTCCTGAGGAGAAAAAAGATAAGAAAAAGAAAAAAATGAGCAAGAAAAAGAAAATTGCTCTCATTGTTTCTTCTGTCATTATCATTTTTATCATCGGTATTTTACTACTGTGGCTTTTAGGCAAGAGCCCTGATGAAGTCGCTGTGCCGGACGTTTCTGGTAAAACGGAAGACCAAGCAGTGGCGTTACTTCAAAAAGAAGGCTTTGTCATAGGTAAAACAGCAGAAAAAAATAGTGATGAAGTAGATGAGGGGAAAGTAATTAATACCGACCCAGAAGCTGGAGAAATGAAAGAAAAAGGAACAAAAATCAATTTATTCGTAAGCATTGGTTCTAAAAAAATAACCATGGATGACTATACAGGAAGAAGTTACACAGATACAAAAGCTTTACTTGAAGAACAGGGATTTAAAAATATTTCTTCGGAGGAAGCTTATAGTTCAGAAATTGAAAAAGGCTTAATTATTAGCCAAACACCGACACAAGGAACAGAAGTAGTCGCTAAATCAACAGATGTGAAATTCGTCGTAAGTAAAGGTGCAGAGCCAATTACACTGAAAGATTTACGAGGTTATACTAAAACAGCAGTAGAAGATTATGCTTCACCACTTGGGCTAAAAGTTTCAAGTAAAGAAGAGAACTCAAGTACAGTGGAAAAAGGACAAGTTATTTCACAGTCCCCATCAGCTGGAACAGCAATGAACTCTGGTGATACAATCGAGATTGTCATTTCTGCTGGACCAAAAGAAAAACAAGTAAAAGAAGTAACTAAAACATTTAACATCCCTTATACACCAAGTGATGAAGAAAACCCACAACCACAGAAAATTCAAATCTACATTCAAGATAAAGACCATAGCATGACTAGCGCTTACCGAGAAATGAGCATTACACAAAATACCTCGGTAGAAATAACTTTCCAAATTGAAGAAGGTTCTAGCGCAGGCTATAAAATTATCAGTGATGATAAAGTAATCGATGAAGGTACAGTCCCTTATCCAAAT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,660 total letters Query= IDP96801.102 Length=2370 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3544 0.0 2_08H_H2_IDP96801.102_T7term.seq 199 7e-54 1_02H_H2_IDP96801.102_T7S.seq 125 1e-31 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 58 TTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGG 147 1 17 ..................................................................... 85 S H P Q F E K G T E N L Y F Q S N A M M I G K R L S D R Y K Query_1 148 TCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGATGATTGGTAAGCGATTAAGCGATCGATATAAG 237 0 1 .................................... 36 1 86 ..N............................ 116 I L H A I G G G G M A N V Y L A H D I I L D R D V A V K I L Query_1 238 ATTTTACATGCAATAGGCGGCGGCGGAATGGCCAATGTGTACTTGGCGCATGATATTATTCTTGATAGAGATGTTGCAGTGAAAATTTTA 327 0 37 .......................................................................................... 126 0 550 ........... 560 R I D L A D E S N L I R R F Q R E A Q S A T S L V H P N I V Query_1 328 CGAATCGATTTGGCAGATGAAAGCAACCTTATTCGTCGGTTCCAACGAGAAGCTCAATCAGCAACAAGTCTCGTTCACCCTAATATTGTT 417 0 127 .......................................................................................... 216 S V Y D V G E E N D L H Y I V M E H V D G M D L K Q Y I H E Query_1 418 AGTGTGTACGATGTTGGTGAAGAAAATGATTTACACTATATTGTAATGGAACATGTAGATGGGATGGATTTAAAACAGTACATTCACGAA 507 0 217 .......................................................................................... 306 N H P I S Y E K A V D I M L Q I V S A V A I A H Q H H I I H Query_1 508 AATCATCCAATCAGTTATGAAAAAGCAGTAGATATTATGCTGCAAATCGTTTCTGCGGTAGCAATCGCCCATCAACATCACATTATCCAC 597 0 307 .......................................................................................... 396 R D L K P Q N I L I D H D G V V K I T D F G I A M A L S E T Query_1 598 CGTGACTTAAAACCGCAAAATATTTTAATTGATCATGATGGTGTCGTGAAGATTACAGATTTTGGTATTGCAATGGCACTTTCAGAAACA 687 0 397 .......................................................................................... 486 S I T Q T N S L L G S V H Y L S P E Q A R G G M A T Q K S D Query_1 688 TCTATTACACAAACGAATTCTTTACTAGGTTCGGTGCATTACTTATCCCCAGAACAAGCACGTGGCGGAATGGCAACACAAAAATCAGAT 777 0 487 .......................................................................................... 576 0 58 ........... 68 I Y S L G I V L Y E L L T G K V P F D G E S A V S I A I K H Query_1 778 ATCTATTCACTTGGCATTGTGTTATACGAACTATTAACTGGAAAAGTACCTTTTGACGGGGAGTCAGCAGTTTCTATAGCTATTAAGCAT 867 0 577 .......................................................................................... 666 L Q A D I P S A R E Q N P E I P Q S L E N I I I K A T A K D Query_1 868 CTGCAAGCAGACATCCCATCCGCAAGAGAACAAAATCCAGAAATTCCACAAAGCTTAGAAAATATTATCATTAAAGCAACGGCGAAAGAT 957 0 667 .......................................................................................... 756 P F L R Y Q N A E E M E K D L Q T C L N K D R L N E P K Y V Query_1 958 CCATTTTTACGTTACCAAAACGCGGAAGAAATGGAAAAAGACTTGCAAACTTGTTTAAATAAAGACCGTTTGAACGAGCCAAAATATGTT 1047 0 757 .......................................................................................... 846 F P T D D G D T K A I P I I A T K D T M Q N L D K T I V P E Query_1 1048 TTCCCAACAGATGATGGTGATACAAAAGCAATTCCAATCATCGCAACAAAAGATACGATGCAAAACCTTGATAAAACGATTGTTCCAGAA 1137 0 847 .......................................................................................... 936 0 1339 ........... 1349 0 1668 ........... 1678 G K V A A A E V V P E E K K D K K K K K M S K K K K I A L I Query_1 1138 GGAAAAGTAGCCGCTGCCGAAGTAGTTCCTGAGGagaaaaaagataagaaaaagaaaaaaatgagcaagaaaaagaaaaTTGCTCTCATT 1227 0 937 .......................................................................................... 1026 V S S V I I I F I I G I L L L W L L G K S P D E V A V P D V Query_1 1228 GTTTCTTCTGTCATTATCATTTTTATCATCGGTATTTTACTACTGTGGCTTTTAGGCAAGAGCCCTGATGAAGTCGCTGTGCCGGACGTT 1317 0 1027 .......................................................................................... 1116 1 244 . 244 S G K T E D Q A V A L L Q K E G F V I G K T A E K N S D E V Query_1 1318 TCTGGTAAAACGGAAGACCAAGCAGTGGCGTTACTTCAAAAAGAAGGCTTTGTCATAGGTAAAACAGCAGaaaaaaaTAGTGATGAAGTA 1407 0 1117 .......................................................................................... 1206 0 1539 ...........T.G..G.. 1557 1 245 .......... 254 D E G K V I N T D P E A G E M K E K G T K I N L F V S I G S Query_1 1408 GATGAGGGGAAAGTAATTAATACCGACCCAGAAGCTGGAGAAATGAAAGAAAAAGGAACAAAAATCAATTTATTCGTAAGCATTGGTTCT 1497 0 1207 .......................................................................................... 1296 K K I T M D D Y T G R S Y T D T K A L L E E Q G F K N I S S Query_1 1498 aaaaaaaTAACCATGGATGACTATACAGGAAGAAGTTACACAGATACAAAAGCTTTACTTGAAGAACAGGGATTTAAAAATATTTCTTCG 1587 0 1297 .......................................................................................... 1386 0 865 ........... 875 0 1594 ........... 1584 E E A Y S S E I E K G L I I S Q T P T Q G T E V V A K S T D Query_1 1588 GAGGAAGCTTATAGTTCAGAAATTGAAAAAGGCTTAATTATTAGCCAAACACCGACACAAGGAACAGAAGTAGTCGCTAAATCAACAGAT 1677 0 1387 .......................................................................................... 1476 V K F V V S K G A E P I T L K D L R G Y T K T A V E D Y A S Query_1 1678 GTGAAATTCGTCGTAAGTAAAGGTGCAGAGCCAATTACACTGAAAGATTTACGAGGTTATACTAAAACAGCAGTAGAAGATTATGCTTCA 1767 0 1477 .......................................................................................... 1566 0 1176 ...........A.A..A.. 1194 P L G L K V S S K E E N S S T V E K G Q V I S Q S P S A G T Query_1 1768 CCACTTGGGCTAAAAGTTTCAAGTAAAGAAGAGAACTCAAGTACAGTGGAAAAAGGACAAGTTATTTCACAGTCCCCATCAGCTGGAACA 1857 0 1567 .......................................................................................... 1656 0 1359 ........... 1349 A M N S G D T I E I V I S A G P K E K Q V K E V T K T F N I Query_1 1858 GCAATGAACTCTGGTGATACAATCGAGATTGTCATTTCTGCTGGACCAAAAGAAAAACAAGTAAAAGAAGTAACTAAAACATTTAACATC 1947 0 1657 .......................................................................................... 1746 0 861 ........... 871 P Y T P S D E E N P Q P Q K I Q I Y I Q D K D H S M T S A Y Query_1 1948 CCTTATACACCAAGTGATGAAGAAAACCCACAACCACAGAAAATTCAAATCTACATTCAAGATAAAGACCATAGCATGACTAGCGCTTAC 2037 0 1747 .......................................................................................... 1836 R E M S I T Q N T S V E I T F Q I E E G S S A G Y K I I S D Query_1 2038 CGAGAAATGAGCATTACACAAAATACCTCGGTAGAAATAACTTTCCAAATTGAAGAAGGTTCTAGCGCAGGCTATAAAATTATCAGTGAT 2127 0 1837 .......................................................................................... 1926 D K V I D E G T V P Y P N * H W K W I T D P N S S S V D K L Query_1 2128 GATAAAGTAATCGATGAAGGTACAGTCCCTTATCCAAATTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTT 2217 0 1927 ....................................... 1965 2 131 ................................................... 81 A A A L E H H H H H H * D P A A N K A R K E Query_1 2218 GCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAA 2283 2 80 ...................................................-..........A... 16 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 8491977 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 3,660 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96804.102 (IDP96804.1-626.F..pMCSG53)
MTTPSHLSDRYELGEILGFGGMSEVHLARDLRLHRDVAVKVLRADLARDPSFYLRFRREAQNAAALNHPAIVAVYDTGEAETPAGPLPYIVMEYVDGVTLRDIVHTEGPMTPKRAIEVIADACQALNFSHQNGIIHRDVKPANIMISATNAVKVMDFGIARAIADSGNSVTQTAAVIGTAQYLSPEQARGDSVDARSDVYSLGCVLYEVLTGEPPFTGDSPVSVAYQHVREDPIPPSARHEGLSADLDAVVLKALAKNPENRYQTAAEMRADLVRVHNGEPPEAPKVLTDAERTSLLSSAAGNLSGPRTDPLPRQDLDDTDRDRSIGSVGRWVAVVAVLAVLTVVVTIAINTFGGITRDVQVPDVRGQSSADAIATLQNRGFKIRTLQKPDSTIPPDHVIGTDPAANTSVSAGDEITVNVSTGPEQREIPDVSTLTYAEAVKKLTAAGFGRFKQANSPSTPELVGKVIGTNPPANQTSAITNVVIIIVGSGPATKDIPDVAGQTVDVAQKNLNVYGFTKFSQASVDSPRPAGEVTGTNPPAGTTVPVDSVIELQVSKGNQFVMPDLSGMFWVDAEPRLRALGWTGMLDKGADVDAGGSQHNRVVYQNPPAGTGVNRDGIITLRFGQ
ATGACCACCCCTTCCCACCTGTCCGACCGCTACGAACTTGGCGAAATCCTTGGATTTGGGGGCATGTCCGAGGTCCACCTGGCCCGCGACCTCCGGTTGCACCGCGACGTTGCGGTCAAGGTGCTGCGCGCTGATCTAGCCCGCGATCCCAGTTTTTACCTTCGCTTCCGGCGTGAGGCGCAAAACGCCGCGGCATTGAACCACCCTGCAATCGTCGCGGTCTACGACACCGGTGAAGCCGAAACGCCCGCCGGGCCATTGCCCTACATCGTCATGGAATACGTCGACGGCGTTACCCTGCGCGACATTGTCCACACCGAAGGGCCGATGACGCCCAAACGCGCCATCGAGGTCATCGCCGACGCCTGCCAAGCGCTGAACTTCAGTCATCAGAACGGAATCATCCACCGTGACGTCAAGCCGGCGAACATCATGATCAGCGCGACCAATGCAGTAAAGGTGATGGATTTCGGCATCGCCCGCGCCATTGCCGACAGCGGCAACAGCGTGACCCAGACCGCAGCAGTGATCGGCACGGCGCAGTACCTGTCACCCGAACAGGCCCGGGGTGATTCCGTCGACGCCCGATCCGATGTCTATTCCTTGGGCTGTGTTCTTTATGAAGTCCTCACCGGGGAGCCACCTTTCACCGGCGACTCACCCGTCTCGGTTGCCTACCAACATGTGCGCGAAGACCCGATCCCACCTTCGGCGCGGCACGAAGGCCTCTCCGCCGACCTGGACGCCGTCGTTCTCAAGGCGCTGGCCAAAAATCCGGAAAACCGCTATCAGACAGCGGCGGAGATGCGCGCCGACCTGGTCCGCGTGCACAACGGTGAGCCGCCCGAGGCGCCCAAAGTGCTCACCGATGCCGAGCGGACCTCGCTGCTGTCGTCTGCGGCCGGCAACCTTAGCGGTCCGCGCACCGATCCGCTACCACGCCAGGACTTAGACGACACCGACCGTGACCGCAGCATCGGTTCGGTGGGCCGTTGGGTTGCGGTGGTCGCCGTGCTCGCTGTGCTGACCGTCGTGGTAACCATCGCCATCAACACGTTCGGCGGCATCACCCGCGACGTTCAAGTTCCCGACGTTCGGGGTCAATCCTCCGCCGACGCCATCGCCACACTGCAAAACCGGGGCTTCAAAATCCGCACCTTGCAGAAGCCGGACTCGACAATCCCACCGGACCACGTTATCGGCACCGACCCGGCCGCCAACACGTCGGTGAGTGCAGGCGACGAGATCACAGTCAACGTGTCCACCGGACCCGAGCAACGCGAAATACCCGACGTCTCCACGCTGACATACGCCGAAGCGGTCAAGAAACTGACTGCCGCCGGATTCGGCCGCTTCAAGCAAGCGAATTCGCCGTCCACCCCGGAACTGGTGGGCAAGGTCATCGGGACCAACCCGCCAGCCAACCAGACGTCGGCCATCACCAATGTGGTCATCATCATCGTTGGCTCTGGTCCGGCGACCAAAGACATTCCCGATGTCGCGGGCCAGACCGTCGACGTGGCGCAGAAGAACCTCAACGTCTACGGCTTCACCAAATTCAGTCAGGCCTCGGTGGACAGCCCCCGTCCCGCCGGCGAGGTGACCGGCACCAATCCACCCGCAGGCACCACAGTTCCGGTCGATTCAGTCATCGAACTACAGGTGTCCAAGGGCAACCAATTCGTCATGCCCGACCTATCCGGCATGTTCTGGGTCGACGCCGAACCACGATTGCGCGCGCTGGGCTGGACCGGGATGCTCGACAAAGGGGCCGACGTCGACGCCGGTGGCTCCCAACACAACCGGGTCGTCTATCAAAACCCGCCGGCGGGGACCGGCGTCAACCGGGACGGCATCATCACGCTGAGGTTCGGCCAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,681 total letters Query= IDP96804.102 Length=2283 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3388 0.0 1_03B_B3_IDP96804.102_T7S.seq 1559 0.0 2_09B_B3_IDP96804.102_T7term.seq 1379 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 58 TTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGG 147 0 1451 ...........G.. 1464 1 19 .............-.......................................................--------------------- 86 2 561 ...........G.. 548 S H P Q F E K G T E N L Y F Q S N A M T T P S H L S D R Y E Query_1 148 TCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGACCACCCCTTCCCACCTGTCCGACCGCTACGAA 237 0 1 .................................... 36 1 87 ---------------------------............................................................... 149 L G E I L G F G G M S E V H L A R D L R L H R D V A V K V L Query_1 238 CTTGGCGAAATCCTTGGATTTGGGGGCATGTCCGAGGTCCACCTGGCCCGCGACCTCCGGTTGCACCGCGACGTTGCGGTCAAGGTGCTG 327 0 37 .......................................................................................... 126 0 1509 ............A..G.. 1492 1 150 .......................................................................................... 239 2 503 ............A..G.. 520 R A D L A R D P S F Y L R F R R E A Q N A A A L N H P A I V Query_1 328 CGCGCTGATCTAGCCCGCGATCCCAGTTTTTACCTTCGCTTCCGGCGTGAGGCGCAAAACGCCGCGGCATTGAACCACCCTGCAATCGTC 417 0 127 .......................................................................................... 216 1 240 .......................................................................................... 329 A V Y D T G E A E T P A G P L P Y I V M E Y V D G V T L R D Query_1 418 GCGGTCTACGACACCGGTGAAGCCGAAACGCCCGCCGGGCCATTGCCCTACATCGTCATGGAATACGTCGACGGCGTTACCCTGCGCGAC 507 0 217 .......................................................................................... 306 1 330 .......................................................................................... 419 I V H T E G P M T P K R A I E V I A D A C Q A L N F S H Q N Query_1 508 ATTGTCCACACCGAAGGGCCGATGACGCCCAAACGCGCCATCGAGGTCATCGCCGACGCCTGCCAAGCGCTGAACTTCAGTCATCAGAAC 597 0 307 .......................................................................................... 396 1 420 .......................................................................................... 509 G I I H R D V K P A N I M I S A T N A V K V M D F G I A R A Query_1 598 GGAATCATCCACCGTGACGTCAAGCCGGCGAACATCATGATCAGCGCGACCAATGCAGTAAAGGTGATGGATTTCGGCATCGCCCGCGCC 687 0 397 .......................................................................................... 486 1 510 .......................................................................................... 599 I A D S G N S V T Q T A A V I G T A Q Y L S P E Q A R G D S Query_1 688 ATTGCCGACAGCGGCAACAGCGTGACCCAGACCGCAGCAGTGATCGGCACGGCGCAGTACCTGTCACCCGAACAGGCCCGGGGTGATTCC 777 0 487 .......................................................................................... 576 1 600 ....................................N..N................-................................. 688 V D A R S D V Y S L G C V L Y E V L T G E P P F T G D S P Query_1 778 GTCGACGCCCGATCCGATGTCTATTCCTTGGGCTGTGTTCTTTATGAAGTCCTCACCGGGGAGCCACC-TTTCACCGGCGACTCACCCGT 866 0 577 ....................................................................-..................... 665 1 689 ...N................................................................T..................... 778 V S V A Y Q H V R E D P I P P S A R H E G L S A D L D A V V Query_1 867 CTCGGTTGCCTACCAACATGTGCGCGAAGACCCGATCCCACCTTCGGCGCGGCACG-AAGGCCTCTCCGCCGACCTGGACGCCGTCGTTC 955 0 666 ........................................................-................................. 754 0 810 ........... 820 1 779 ...................................................N....A................................. 868 L K A L A K N P E N R Y Q T A A E M R A D L V R V H N G E Query_1 956 TCAAGGCGCTGGCCAAAAATCCGG-AAAACCGCT-ATCAGACAGCGGCGGAGATGCGCGCCGA--CCTGGTCCGCGTGCACAACGGTGAG 1041 0 755 ........................-.........-............................--......................... 840 0 732 ......--..... 742 1 869 ..N.....................A.........A.........T..................AC.T.......N..........N.... 958 1 846 ......--..... 856 P P E A P K V L T D A E R T S L L S S A A G N L S G P R T Query_1 1042 CCGCCCG-AGGCGCCCAAAGTGC-TCACCGATGCCGAGCGGACCTCGCTGCTGTCGTCTGCGGCCGGCAACCTTAGCGGTCCGCGCACCG 1129 0 841 .......-...............-.................................................................. 928 1 959 T......A...............T............. 995 D P L P R Q D L D D T D R D R S I G S V G R W V A V V A V L Query_1 1130 ATCCGCTACCACGCCAGGACTTAGACGACACCGACCGTGACCGCAGCATCGGTTCGGTGGGCCGTTGGGTTGCGGTGGTCGCCGTGCTCG 1219 0 929 .......................................................................................... 1018 A V L T V V V T I A I N T F G G I T R D V Q V P D V R G Q S Query_1 1220 CTGTGCTGACCGTCGTGGTAACCATCGCCATCAACACGTTCGGCGGCATCACCCGCGACGTTCAAGTTCCCGACGTTCGGGGTCAATCCT 1309 0 1019 .......................................................................................... 1108 S A D A I A T L Q N R G F K I R T L Q K P D S T I P P D H V Query_1 1310 CCGCCGACGCCATCGCCACACTGCAAAACCGGGGCTTCAAAATCCGCACCTTGCAGAAGCCGGACTCGACAATCCCACCGGACCACGTTA 1399 0 1109 .......................................................................................... 1198 I G T D P A A N T S V S A G D E I T V N V S T G P E Q R E Query_1 1400 TCGGCACCGACCCGGCCGCCAACACGTCGGTGAGTGCAGGCGACGAGATCACAGTCAA-CGTGTCCACCGGACCCGAGCAA-CGCGAAAT 1487 0 1199 ..........................................................-......................-........ 1286 2 804 ..............................................A......................A........ 727 I P D V S T L T Y A E A V K K L T A A G F G R F K Q A N S P Query_1 1488 ACCCGACGTCTCCACGCTGACATACGCCGAAGCGGTCAAGAAA-CTGACTGCCGCCGGATTCGGCCGCTTCAAGCAAGCGAATTCGCCGT 1576 0 1287 ...........................................-.............................................. 1375 2 726 ...........................................A.............................................. 637 S T P E L V G K V I G T N P P A N Q T S A I T N V V I I I V Query_1 1577 CCACCCCGGAACTGGTGGGCAAGGTCATCGGGACCAACCCGCCAGCCAACCAGACGTCGGCCATCACCAATGTGGTCATCATCATCGTTG 1666 0 1376 .......................................................................................... 1465 1 74 ........... 84 2 636 .......................................................................................... 547 G S G P A T K D I P D V A G Q T V D V A Q K N L N V Y G F T Query_1 1667 GCTCTGGTCCGGCGACCAAAGACATTCCCGATGTCGCGGGCCAGACCGTCGACGTGGCGCAGAAGAACCTCAACGTCTACGGCTTCACCA 1756 0 1466 .......................................................................................... 1555 0 96 ..G..G............ 79 1 209 ..G..G............ 192 2 546 .......................................................................................... 457 K F S Q A S V D S P R P A G E V T G T N P P A G T T V P V D Query_1 1757 AATTCAGTCAGGCCTCGGTGGACAGCCCCCGTCCCGCCGGCGAGGTGACCGGCACCAATCCACCCGCAGGCACCACAGTTCCGGTCGATT 1846 0 1556 .......................................................................................... 1645 2 456 ....................................T..................................................... 367 S V I E L Q V S K G N Q F V M P D L S G M F W V D A E P R L Query_1 1847 CAGTCATCGAACTACAGGTGTCCAAGGGCAACCAATTCGTCATGCCCGACCTATCCGGCATGTTCTGGGTCGACGCCGAACCACGATTGC 1936 0 1646 .......................................................................................... 1735 2 366 .......................................................................................... 277 R A L G W T G M L D K G A D V D A G G S Q H N R V V Y Q N P Query_1 1937 GCGCGCTGGGCTGGACCGGGATGCTCGACAAAGGGGCCGACGTCGACGCCGGTGGCTCCCAACACAACCGGGTCGTCTATCAAAACCCGC 2026 0 1736 .......................................................................................... 1825 0 1832 ..... 1828 2 276 .......................................................................................... 187 2 180 ..... 184 P A G T G V N R D G I I T L R F G Q * H W K W I T D P N S S Query_1 2027 CGGCGGGGACCGGCGTCAACCGGGACGGCATCATCACGCTGAGGTTCGGCCAGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCT 2116 0 1826 ..................................................... 1878 0 1827 ....... 1821 2 186 .......................................................................................... 97 2 185 ....... 191 S V D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 2117 CCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAAC-AAAGCCCGAAAGGAA 2196 2 96 .................................................................A...N.......A... 16 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 8225580 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 3,681 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96806.102 (IDP96806.1-664.F..pMCSG53)
MIGKIINERYKIVDKLGGGGMSTVYLAEDTILNIKVAIKAIFIPPREKEETLKRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYIESHGPLSVDTAINFTNQILDGIKHAHDMRIVHRDIKPQNILIDSNKTLKIFDFGIAKALSETSLTQTNHVLGTVQYFSPEQAKGEATDECTDIYSIGIVLYEMLVGEPPFNGETAVSIAIKHIQDSVPNVTTDVRKDIPQSLSNVILRATEKDKANRYKTIQEMKDDLSSVLHENRANEDVYELDKMKTIAVPLKKEDLAKHISEHKSNQPKRETTQVPIVNGPAHHQQFQKPEGTVYEPKPKKKSTRKIVLLSLIFSLLMIALVSFVAMAMFGNKYEETPDVIGKSVKEAEQIFNKNNLKLGKISRSYSDKYPENEIIKTTPNTGERVERGDSVDVVISKGPEKVKMPNVIGLPKEEALQKLKSLGLKDVTIEKVYNNQAPKGYIANQSVTANTEIAIHDSNIKLYESLGIKQVYVEDFEHKSFSKAKKALEEKGFKVESKEEYSDDIDEGDVISQSPKGKSVDEGSTISFVVSKGKKSDSSDVKTTTESVDVPYTGKNDKSQKVKVYIKDKDNDGSTEKGSFDITSDQRIDIPLRIEKGKTASYIVKVDGKTVAEKEVSYDDV
ATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGTACATAACTCATCACAGCTATCACATCAAAATATAGTAAGTATGATCGATGTTGATGAAGAAGATGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATGCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATCATGTGTTAGGTACTGTGCAGTACTTTTCGCCAGAACAAGCAAAAGGTGAGGCAACGGATGAATGTACAGATATTTATTCTATAGGTATTGTGTTATATGAAATGCTTGTTGGTGAACCACCCTTTAATGGAGAAACTGCAGTTAGCATTGCGATTAAACATATTCAGGATTCTGTGCCAAATGTGACAACAGATGTACGTAAGGATATTCCGCAATCTTTAAGTAATGTCATTTTACGCGCTACAGAAAAAGACAAAGCGAATCGTTACAAAACAATTCAAGAAATGAAAGATGATTTGAGTAGTGTTTTACATGAAAATCGAGCGAATGAAGATGTCTATGAACTCGATAAAATGAAAACGATAGCGGTACCTTTGAAAAAAGAAGATCTAGCAAAGCATATTAGTGAACATAAGTCGAATCAACCTAAACGTGAAACGACGCAAGTACCTATTGTAAATGGGCCTGCTCATCATCAGCAATTCCAAAAGCCAGAAGGTACGGTGTACGAACCAAAACCTAAAAAGAAATCAACACGAAAGATTGTGCTCTTATCACTAATCTTTTCGTTGTTAATGATTGCACTTGTTTCTTTTGTGGCAATGGCAATGTTTGGTAATAAATACGAAGAGACACCTGATGTAATCGGGAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATCCTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGTTACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATTAAGCAAGTTTATGTAGAAGACTTTGAACATAAATCCTTTAGCAAAGCTAAAAAAGCCTTAGAAGAAAAAGGGTTTAAAGTTGAAAGTAAGGAAGAGTATAGTGACGATATTGATGAGGGTGATGTGATTTCTCAATCTCCTAAAGGAAAATCAGTAGATGAGGGGTCAACGATTTCATTTGTTGTTTCTAAAGGTAAAAAAAGTGACTCATCAGATGTCAAAACGACAACTGAATCGGTAGATGTACCATACACTGGTAAAAATGATAAGTCACAAAAAGTTAAAGTTTATATTAAAGATAAAGATAATGACGGTTCAACTGAAAAAGGTAGTTTCGATATTACTAGTGATCAACGTATAGACATTCCTTTAAGAATTGAAAAAGGAAAAACAGCAAGTTATATTGTTAAAGTTGACGGTAAAACTGTAGCTGAAAAAGAAGTCAGCTATGATGATGTA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,219 total letters Query= IDP96806.102 Length=2397 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3593 0.0 1_07F_F7_IDP96806.102_T7S.seq 26.5 0.078 M I G K I I N E R Y K I V D K L G G G G M S T V Y L A E D T Query_1 202 ATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACG 291 0 1 .......................................................................................... 90 I L N I K V A I K A I F I P P R E K E E T L K R F E R E V H Query_1 292 ATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGTACAT 381 0 91 .......................................................................................... 180 N S S Q L S H Q N I V S M I D V D E E D D C Y Y L V M E Y I Query_1 382 AACTCATCACAGCTATCACATCAAAATATAGTAAGTATGATCGATGTTGATGAAGAAGATGACTGTTACTACTTAGTAATGGAATATATC 471 0 181 .......................................................................................... 270 E G P T L S E Y I E S H G P L S V D T A I N F T N Q I L D G Query_1 472 GAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGC 561 0 271 .......................................................................................... 360 0 454 ........... 444 I K H A H D M R I V H R D I K P Q N I L I D S N K T L K I F Query_1 562 ATTAAACATGCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTT 651 0 361 .......................................................................................... 450 0 353 ....... 347 D F G I A K A L S E T S L T Q T N H V L G T V Q Y F S P E Q Query_1 652 GATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATCATGTGTTAGGTACTGTGCAGTACTTTTCGCCAGAACAA 741 0 451 .......................................................................................... 540 0 992 ............. 980 0 346 .... 343 A K G E A T D E C T D I Y S I G I V L Y E M L V G E P P F N Query_1 742 GCAAAAGGTGAGGCAACGGATGAATGTACAGATATTTATTCTATAGGTATTGTGTTATATGAAATGCTTGTTGGTGAACCACCCTTTAAT 831 0 541 .......................................................................................... 630 G E T A V S I A I K H I Q D S V P N V T T D V R K D I P Q S Query_1 832 GGAGAAACTGCAGTTAGCATTGCGATTAAACATATTCAGGATTCTGTGCCAAATGTGACAACAGATGTACGTAAGGATATTCCGCAATCT 921 0 631 .......................................................................................... 720 L S N V I L R A T E K D K A N R Y K T I Q E M K D D L S S V Query_1 922 TTAAGTAATGTCATTTTACGCGCTACAGAAAAAGACAAAGCGAATCGTTACAAAACAATTCAAGAAATGAAAGATGATTTGAGTAGTGTT 1011 0 721 .......................................................................................... 810 L H E N R A N E D V Y E L D K M K T I A V P L K K E D L A K Query_1 1012 TTACATGAAAATCGAGCGAATGAAGATGTCTATGAACTCGATAAAATGAAAACGATAGCGGTACCTTTGAAAAAAGAAGATCTAGCAAAG 1101 0 811 .......................................................................................... 900 H I S E H K S N Q P K R E T T Q V P I V N G P A H H Q Q F Q Query_1 1102 CATATTAGTGAACATAAGTCGAATCAACCTAAACGTGAAACGACGCAAGTACCTATTGTAAATGGGCCTGCTCATCATCAGCAATTCCAA 1191 0 901 .......................................................................................... 990 0 465 ........... 455 K P E G T V Y E P K P K K K S T R K I V L L S L I F S L L M Query_1 1192 AAGCCAGAAGGTACGGTGTACGAACCAAAACCTAAAAAGAAATCAACACGAAAGATTGTGCTCTTATCACTAATCTTTTCGTTGTTAATG 1281 0 991 .......................................................................................... 1080 0 454 .. 453 I A L V S F V A M A M F G N K Y E E T P D V I G K S V K E A Query_1 1282 ATTGCACTTGTTTCTTTTGTGGCAATGGCAATGTTTGGTAATAAATACGAAGAGACACCTGATGTAATCGGGAAATCTGTAAAAGAAGCA 1371 0 1081 .......................................................................................... 1170 E Q I F N K N N L K L G K I S R S Y S D K Y P E N E I I K T Query_1 1372 GAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATCCTGAAAATGAAATTATTAAGACA 1461 0 1171 .......................................................................................... 1260 T P N T G E R V E R G D S V D V V I S K G P E K V K M P N V Query_1 1462 ACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTC 1551 0 1261 .......................................................................................... 1350 I G L P K E E A L Q K L K S L G L K D V T I E K V Y N N Q A Query_1 1552 ATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGTTACGATTGAAAAAGTATATAATAATCAAGCG 1641 0 1351 .......................................................................................... 1440 P K G Y I A N Q S V T A N T E I A I H D S N I K L Y E S L G Query_1 1642 CCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGC 1731 0 1441 .......................................................................................... 1530 I K Q V Y V E D F E H K S F S K A K K A L E E K G F K V E S Query_1 1732 ATTAAGCAAGTTTATGTAGAAGACTTTGAACATAAATCCTTTAGCAAAGCTAAAAAAGCCTTAGAAGAAAAAGGGTTTAAAGTTGAAAGT 1821 0 1531 .......................................................................................... 1620 1 81 ..T......-- 89 K E E Y S D D I D E G D V I S Q S P K G K S V D E G S T I S Query_1 1822 AAGGAAGAGTATAGTGACGATATTGATGAGGGTGATGTGATTTCTCAATCTCCTAAAGGAAAATCAGTAGATGAGGGGTCAACGATTTCA 1911 0 1621 .......................................................................................... 1710 1 90 ............ 101 F V V S K G K K S D S S D V K T T T E S V D V P Y T G K N D Query_1 1912 TTTGTTGTTTCTAAAGGTaaaaaaaGTGACTCATCAGATGTCAAAACGACAACTGAATCGGTAGATGTACCATACACTGGTAAAAATGAT 2001 0 1711 .......................................................................................... 1800 K S Q K V K V Y I K D K D N D G S T E K G S F D I T S D Q R Query_1 2002 AAGTCACAAAAAGTTAAAGTTTATATTAAAGATAAAGATAATGACGGTTCAACTGAAAAAGGTAGTTTCGATATTACTAGTGATCAACGT 2091 0 1801 .......................................................................................... 1890 I D I P L R I E K G K T A S Y I V K V D G K T V A E K E V Query_1 2092 ATAGACATTCCTTTAAGAATT-GAAAAAGGAAAAACAGCAAGTTATATTGTTAAAGTTGACGGTAAAACTGTAGCTGAAAAAGAAGTCAG 2180 0 1891 .....................-.................................................................... 1979 1 74 ...C..T....T.......... 95 S Y D D V Query_1 2181 CTATGATGATGTA 2193 0 1980 ............. 1992 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 7539840 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 3,219 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96845.102 (IDP96845.1-182.F..pMCSG53)
MTQPISVIAKSLVRERARTGLSLAEVARRAGIAKSTLSQLESGNGNPSLETLWSLCVALDIPFARLLEPQVNKTQVIRRGEGTKVVAEQANYQAILLAACPPGARRDIYLLLTQPGADRISHPHPPGSVEHIIVTQGRARVGLTSAPEELGEGDYICYPADQEHVFQALEPDTQALLIAEQN
ATGACACAGCCAATCAGCGTTATCGCCAAAAGTCTGGTGCGGGAGCGGGCGCGGACCGGGCTTTCGCTGGCGGAAGTCGCGCGTCGGGCGGGTATCGCAAAATCCACGCTATCGCAACTGGAGTCCGGCAACGGCAACCCGAGTCTGGAAACGCTGTGGTCGCTCTGCGTAGCGCTGGATATTCCATTTGCCAGGCTGCTTGAGCCGCAGGTGAACAAAACGCAGGTGATCCGCCGCGGCGAGGGGACCAAAGTGGTGGCAGAGCAGGCGAACTATCAGGCCATTTTGCTGGCCGCCTGTCCGCCCGGCGCGCGTCGGGACATCTATTTGCTGCTCACCCAGCCCGGCGCCGACCGCATTTCGCATCCGCATCCGCCAGGCTCGGTGGAACATATTATCGTCACCCAGGGGCGGGCGCGGGTAGGGCTAACGTCGGCGCCGGAAGAGCTCGGCGAGGGCGACTATATCTGCTACCCGGCGGATCAGGAGCATGTTTTTCAGGCGCTGGAGCCGGATACGCAGGCCCTGCTGATCGCTGAGCAAAAC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,612 total letters Query= IDP96845.102 Length=951 Score E Sequences producing significant alignments: (Bits) Value 0_wt 985 0.0 2_07G_G1_IDP96845.102_T7term.seq 194 8e-53 1_01G_G1_IDP96845.102_T7S.seq 104 1e-25 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 58 TTCCCCTCTAGAAATAATTTT-GTTTAACTTT-AAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGT 145 1 18 .........NA..........T..........T...................................... 88 W S H P Q F E K G T E N L Y F Q S N A M T Q P I S V I A K S Query_1 146 GGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGACACAGCCAATCAGCGTTATCGCCAAAAGTC 235 0 1 .................................. 34 1 89 .............................. 118 L V R E R A R T G L S L A E V A R R A G I A K S T L S Q L E Query_1 236 TGGTGCGGGAGCGGGCGCGGACCGGGCTTTCGCTGGCGGAAGTCGCGCGTCGGGCGGGTATCGCAAAATCCACGCTATCGCAACTGGAGT 325 0 35 .......................................................................................... 124 0 309 ........... 319 S G N G N P S L E T L W S L C V A L D I P F A R L L E P Q V Query_1 326 CCGGCAACGGCAACCCGAGTCTGGAAACGCTGTGGTCGCTCTGCGTAGCGCTGGATATTCCATTTGCCAGGCTGCTTGAGCCGCAGGTGA 415 0 125 .......................................................................................... 214 N K T Q V I R R G E G T K V V A E Q A N Y Q A I L L A A C P Query_1 416 ACAAAACGCAGGTGATCCGCCGCGGCGAGGGGACCAAAGTGGTGGCAGAGCAGGCGAACTATCAGGCCATTTTGCTGGCCGCCTGTCCGC 505 0 215 .......................................................................................... 304 P G A R R D I Y L L L T Q P G A D R I S H P H P P G S V E H Query_1 506 CCGGCGCGCGTCGGGACATCTATTTGCTGCTCACCCAGCCCGGCGCCGACCGCATTTCGCATCCGCATCCGCCAGGCTCGGTGGAACATA 595 0 305 .......................................................................................... 394 0 78 ........... 88 0 442 ........... 432 2 236 ........... 226 I I V T Q G R A R V G L T S A P E E L G E G D Y I C Y P A D Query_1 596 TTATCGTCACCCAGGGGCGGGCGCGGGTAGGGCTAACGTCGGCGCCGGAAGAGCTCGGCGAGGGCGACTATATCTGCTACCCGGCGGATC 685 0 395 .......................................................................................... 484 0 354 ........... 344 2 341 ........... 331 Q E H V F Q A L E P D T Q A L L I A E Q N * H W K W I T D P Query_1 686 AGGAGCATGTTTTTCAGGCGCTGGAGCCGGATACGCAGGCCCTGCTGATCGCTGAGCAAAACTAACATTGGAAGTGGATAACGGATCCGA 775 0 485 .............................................................. 546 2 135 ............................ 108 N S S S V D K L A A A L E H H H H H H * D P A A N K A R K Query_1 776 ATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAA--GCCCGAAAGGA 863 2 107 .............................................................................AGC........A. 18 E Query_1 864 A 864 2 17 . 17 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2402712 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 2,612 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96847.102 (IDP96847.1-440.F..pMCSG53)
MKKFSVVIAGGGSTFTPGIVLMLLANQDRFPLRSLKFYDNDGARQETIAEACKVILKEQAPEIEFSYTTDPQAAFTDVDFVMAHIRVGKYPMREQDEKIPLRHGVLGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLRPNAKILNICDMPIGIEGRMAQIVGLKDRKQMRVRYYGLNHFGWWTSIEDLDGNDLMPKLREYVAKYGYVPPSNDPHTEASWNDTFAKAKDVQALDPQTMPNTYLKYYLFPDYVVAHSNPERTRANEVMDHREKNVFSACRAIIAAGKSTAGDLEIDEHASYIVDLATAIAFNTQERMLLIVPNNGAIHNFDADAMVEIPCLVGHNGPEPLTVGDIPHFQKGLMSQQVAVEKLVVDAWEQRSYHKLWQAITLSKTVPSASVAKAILDDLIAANKDYWPELH
ATGAAAAAATTCTCAGTTGTTATCGCAGGCGGCGGCAGCACCTTCACCCCGGGCATTGTCCTGATGCTGCTGGCAAACCAGGATCGTTTTCCGCTGCGGTCGCTGAAATTTTACGACAACGACGGCGCGCGCCAGGAGACCATCGCCGAGGCGTGCAAAGTGATCCTCAAAGAGCAGGCGCCGGAGATTGAGTTCAGCTATACCACCGATCCGCAGGCGGCGTTTACTGATGTTGATTTTGTCATGGCGCATATCCGCGTCGGGAAATATCCGATGCGTGAACAGGATGAGAAAATCCCGCTACGCCACGGCGTGCTGGGCCAGGAGACCTGCGGACCGGGCGGGATCGCCTACGGTATGCGCTCTATCGGCGGCGTGCTGGAGCTGGTGGATTATATGGAAAAATATTCGCCGAACGCCTGGATGCTTAACTACTCCAACCCGGCGGCGATTGTGGCGGAAGCCACTCGTCGTCTGCGGCCGAACGCCAAAATCCTCAACATCTGCGATATGCCGATCGGCATTGAAGGGCGGATGGCGCAGATTGTCGGCCTCAAAGACCGTAAACAGATGCGCGTGCGCTACTACGGCCTCAACCATTTCGGCTGGTGGACCTCGATTGAGGACCTCGACGGCAATGATTTGATGCCGAAACTGCGCGAATATGTGGCGAAATACGGCTATGTGCCGCCCTCGAACGATCCGCACACCGAGGCCAGCTGGAACGACACCTTTGCCAAAGCGAAAGATGTGCAGGCGCTGGATCCGCAGACCATGCCGAACACCTACCTGAAATATTATCTGTTCCCGGACTATGTGGTAGCCCACTCCAACCCGGAACGCACCCGCGCCAATGAGGTGATGGATCATCGGGAGAAAAACGTCTTCAGCGCCTGCCGGGCGATCATCGCCGCCGGGAAATCCACCGCCGGCGATCTGGAGATTGACGAGCATGCCTCCTATATCGTCGATCTGGCGACGGCTATCGCCTTTAACACGCAGGAAAGGATGCTGCTGATTGTGCCGAACAATGGCGCGATCCATAACTTTGACGCCGACGCGATGGTGGAGATCCCTTGTCTGGTAGGCCACAACGGACCGGAGCCGCTGACGGTAGGCGACATCCCGCATTTCCAGAAAGGGCTGATGAGCCAGCAGGTGGCAGTGGAAAAACTGGTGGTCGATGCCTGGGAACAGCGCTCTTACCACAAGCTGTGGCAGGCGATTACCCTGTCGAAAACCGTGCCGAGCGCCTCGGTGGCGAAAGCGATCCTCGACGACCTCATTGCCGCCAACAAAGATTACTGGCCTGAGCTGCAT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,617 total letters Query= IDP96847.102 Length=1725 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2381 0.0 1_02A_A2_IDP96847.102_T7S.seq 1748 0.0 2_08A_A2_IDP96847.102_T7term.seq 1745 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 60 CCCCTCTAGAAATAATTTTGTTT-AACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGT 148 1 20 .......N...-...........T........................................N...---------------------- 86 S H P Q F E K G T E N L Y F Q S N A M K K F S V V I A G G G Query_1 149 CTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGAAAAAATTCTCAGTTGTTATCGCAGGCGGCGGCA 238 0 1 ..................................... 37 0 212 ........... 222 1 87 --------------------------................................................................ 150 1 325 ........... 335 S T F T P G I V L M L L A N Q D R F P L R S L K F Y D N D G Query_1 239 GCACCTTCACCCCGGGCATTGTCCTGATGCTGCTGGCAAACCAGGATCGTTTTCCGCTGCGGTCGCTGAAATTTTACGACAACGACGGCG 328 0 38 .......................................................................................... 127 0 308 ...... 313 1 151 .......................................................................................... 240 1 421 ...... 426 A R Q E T I A E A C K V I L K E Q A P E I E F S Y T T D P Query_1 329 CGC---GCCAGGAGACCATCGCCGAGGCGTGCAAAGTGATCCTCAAAGAGCAGGCGCCGGAGATTGAGTTCAGCTATACCACCGATCCGC 415 0 128 ...---.................................................................................... 214 0 314 T..TGG........... 330 0 24 ... 26 1 241 ...---.................................................................................... 327 1 427 T..TGG........... 443 1 137 ... 139 Q A A F T D V D F V M A H I R V G K Y P M R E Q D E K I P L Query_1 416 AGGCGGCGTTTACTGATGTTGATTTTGTCATGGCGCATATCCGCGTCGGGAAATATCCGATGCGTGAACAGGATGAGAAAATCCCGCTAC 505 0 215 .......................................................................................... 304 0 27 ........ 34 1 328 .......................................................................................... 417 1 140 ........ 147 R H G V L G Q E T C G P G G I A Y G M R S I G G V L E L V D Query_1 506 GCCACGGCGTGCTGGGCCAGGAGACCTGCGGACCGGGCGGGATCGCCTACGGTATGCGCTCTATCGGCGGCGTGCTGGAGCTGGTGGATT 595 0 305 .......................................................................................... 394 0 122 ......C..---........... 141 0 309 ........... 319 0 372 ........... 382 1 418 .......................................................................................... 507 1 235 ......C..---........... 254 1 422 ........... 432 Y M E K Y S P N A W M L N Y S N P A A I V A E A T R R L R P Query_1 596 ATATGGAAAAATATTCGCCGAACGCCTGGATGCTTAACTACTCCAACCCGGCGGCGATTGTGGCGGAAGCCACTCGTCGTCTGCGGCCGA 685 0 395 .......................................................................................... 484 0 827 ............ 838 0 918 ............ 907 1 508 .......................................................................................... 597 1 1035 ........... 1025 2 993 ...................... 972 2 538 ............ 549 2 629 ............ 618 N A K I L N I C D M P I G I E G R M A Q I V G L K D R K Q M Query_1 686 ACGCCAAAATCCTCAACATCTGCGATATGCCGATCGGCATTGAAGGGCGGATGGCGCAGATTGTCGGCCTCAAAGACCGTAAACAGATGC 775 0 485 .......................................................................................... 574 0 524 .............. 511 1 598 .......................................................................................... 687 1 637 .............. 624 2 971 .......................................................................................... 882 2 932 .............. 945 R V R Y Y G L N H F G W W T S I E D L D G N D L M P K L R E Query_1 776 GCGTGCGCTACTACGGCCTCAACCA-TTTCGGCTGGTGGACCTCGATTGAGGACCTCGACGGCAATGATTTGATGCCGAAACTGCGCGAA 864 0 575 .........................-................................................................ 663 1 688 .........................T................................................................ 777 2 881 .........................-................................................................ 793 Y V A K Y G Y V P P S N D P H T E A S W N D T F A K A K D Query_1 865 TATGTGGCGAAATACGGCTATGTGCCGCCCTCGAACGATCCGCACACCGAGGCCAGCTGGAACGACACCTTTGCCAAAGCG-AAAGATGT 953 0 664 .................................................................................-........ 752 1 778 .................................................................................A........ 867 2 792 .................................................................................-........ 704 V Q A L D P Q T M P N T Y L K Y Y L F P D Y V V A H S N P E Query_1 954 GCAGGCGCT-GGATCCGCAGACCATGCCGAACACCTACCTGAAATATTATCTGTTCCCGGACTATGTGGTAGCCCACTCCAACCCGGAAC 1042 0 753 .........-................................................................................ 841 0 434 ............ 445 1 868 ...N.....N................................................N.....................-......... 956 1 547 ............ 558 2 703 .........-................................................................................ 615 R T R A N E V M D H R E K N V F S A C R A I I A A G K S T Query_1 1043 GCACCCGCGCCAATG-AGGTGATGG-ATCATCGGGAG-AAAAACGTCTTCAGCGCCTGCCGGGCGATCATCGCCGCCGGGAAATCCA-CC 1128 0 842 ...............-.........-...........-.................................................-.. 927 0 452 ............ 441 1 957 ...............A.........A...........A....................N....................A.......C.. 1046 1 565 ............ 554 2 614 ...............-.........-...........-.................................................-.. 529 A G D L E I D E H A S Y I V D L A T A I A F N T Q E R Query_1 1129 GCCGGCGAT-CTGG-AGATTG-ACGAGCATGCCT-CCTATATCGTCGATC-TGGCGACGGCTA-TCGCC-TTTAACACGC-AGGAAAGGA 1210 0 928 .........-....-......-............-...............-............-.....-..........-......... 1009 1 1047 .........C....A.A....A....N.......N...............T.........N..T.....N...N......A......... 1136 2 528 .........-....-......-............-...............-............-.....-..........-......... 447 M L L I V P N N G A I H N F D A D A M V E I P C L V G H Query_1 1211 T-GCT-GCT--GATTGTGCC-GAACAATGGCGCGATCCATAACTTTGACGCCGACGCGATGGTGGAGATCCCTTGTCTGGTAGGCCACAA 1295 0 1010 .-...-...--.........-..................................................................... 1094 1 1137 NA...N...GA.......N.G...AN..... 1167 2 446 .-...-...--.........-..................................................................... 362 N G P E P L T V G D I P H F Q K G L M S Q Q V A V E K L V V Query_1 1296 CGGACCGGAGCCGCTGACGGTAGGCGACATCCCGCATTTCCAGAAAGGGCTGATGAGCCAGCAGGTGGCAGTGGAAAAACTGGTGGTCGA 1385 0 1095 .......................................................................................... 1184 2 361 .......................................................................................... 272 D A W E Q R S Y H K L W Q A I T L S K T V P S A S V A K A I Query_1 1386 TGCCTGGGAACAGCGCTCTTACCACAAGCTGTGGCAGGCGATTACCCTGTCGAAAACCGTGCCGAGCGCCTCGGTGGCGAAAGCGATCCT 1475 0 1185 .......................................................................................... 1274 2 271 .......................................................................................... 182 L D D L I A A N K D Y W P E L H * H W K W I T D P N S S S V Query_1 1476 CGACGACCTCATTGCCGCCAACAAAGATTACTGGCCTGAGCTGCATTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGA 1565 0 1275 .............................................. 1320 2 181 .......................................................................................... 92 D K L A A A L E H H H H H H * D P A A N K A R Query_1 1566 CAAGCTTGCGGCCGCACTCGAGcaccaccaccaccacc-acTGAGATCCGGCTGCTAACAAAGCCCGAAA 1634 2 91 ......................................A............................... 22 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 6099421 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 3,617 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96849.102 (IDP96849.1-930.F..pMCSG53)
MVQIPENPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLNMLRSLILQYQPTHAVVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHKMVKAMGLPLMAVPGVEADDVIGTLAREAERAGRPVLISTGDKDMAQLVTPGITLINTMTNTILGPDEVVTKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEPEKIAELSFRGAKTMAAKLEQNKDVAYLSYQLATIKTDVELELTCEELEVQPPAADDLLALFRQYEFKRWTTDVEAGKWLQAKGGKPAAKPAVPAAAAEAEEEVEAATALSAEHYVTILDEATLLTWIDKLKQAPLFAFDTETDSLDNISANMVGLSFAVEPGVAAYVPVAHDYLDAPDQIPRERVLTLLKPLLEDEKVLKVGQNLKYDRGILANYDIELRGIAFDTMLESYILDSVAGRHDMDSLSDRWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQLHLKMWPKLQQHEGPLNIFQHIEMPLVPVLSRVERNGVKIDPAVLHAHSQEIAQRLVELEQRAHEIAGEAFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLPKVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVRNEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAEVFGLPLDSVSSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGVLEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAGAERAAINAPMQGTAADIIKRAMIAVDEWLRSEKPRVRMIMQVHDELVFEVHKDELDAVSKKIHELMENSTTLAVPLLVEVGSGENWDQAH
ATGGTTCAGATCCCAGAAAACCCTCTCATCCTCGTTGACGGCTCCTCCTATCTTTATCGGGCGTATCATGCGTTTCCGCCGCTGACTAACAGCGCAGGGGAGCCCACCGGGGCTATGTACGGCGTACTGAATATGCTGCGCAGTTTGATACTGCAGTATCAACCGACTCATGCCGTCGTGGTCTTTGATGCGAAAGGGAAAACCTTCCGTGATGAATTGTTCGAGCATTACAAATCTCACCGCCCGCCGATGCCGGACGATCTGCGCGCGCAGATTGAGCCGTTGCATAAGATGGTGAAAGCGATGGGGCTGCCGCTGATGGCTGTCCCGGGCGTCGAAGCTGACGATGTGATTGGCACCCTGGCGCGGGAAGCGGAAAGGGCAGGTCGCCCAGTCCTGATCAGCACCGGCGATAAAGACATGGCCCAGCTGGTGACGCCGGGCATCACCCTGATCAATACCATGACCAACACTATCCTCGGACCGGATGAGGTGGTTACTAAGTACGGCGTCCCGCCGGAGCTGATTATTGATTTCCTGGCGCTGATGGGCGACTCCTCGGATAACATTCCCGGCGTGCCGGGCGTGGGCGAAAAGACCGCCCAGGCGCTGCTGCAAGGCCTCGGCGGCCTTGATACGCTGTATGCGGAACCGGAGAAGATCGCTGAACTGAGCTTCCGCGGCGCGAAAACGATGGCGGCGAAGCTCGAGCAGAACAAGGACGTGGCTTATCTCTCTTATCAGCTGGCGACCATCAAAACCGACGTCGAGCTGGAGCTCACCTGCGAAGAGCTGGAGGTGCAGCCGCCGGCGGCAGACGATCTGCTGGCGCTGTTCAGACAGTATGAATTTAAGCGCTGGACCACTGACGTTGAGGCCGGAAAATGGCTGCAGGCTAAGGGGGGCAAACCGGCTGCGAAACCTGCAGTGCCTGCAGCAGCGGCAGAAGCGGAAGAAGAGGTTGAAGCCGCTACTGCACTTTCAGCAGAGCATTATGTCACTATCCTTGATGAGGCTACGCTGCTCACCTGGATCGATAAGCTGAAGCAGGCGCCGCTCTTTGCCTTTGATACGGAAACCGACAGCCTGGATAACATCTCCGCCAACATGGTTGGTCTTTCATTCGCTGTTGAGCCGGGCGTGGCCGCCTATGTCCCGGTAGCGCATGATTACCTTGATGCGCCGGATCAGATCCCGCGCGAGCGCGTGCTGACGCTGCTTAAACCGCTGCTGGAGGATGAGAAGGTGCTCAAGGTCGGGCAAAATCTCAAATACGATCGCGGCATTTTGGCTAATTACGACATTGAGCTGCGCGGGATTGCGTTCGATACCATGCTCGAGTCTTACATTCTCGACAGCGTGGCTGGCCGCCACGATATGGACAGTCTTTCCGACCGCTGGCTGAAACATAAAACCATTACCTTCGAAGAGATCGCTGGCAAGGGTAAGAATCAGCTCACCTTTAACCAGATTGCGCTGGAAGAGGCTGGTCGCTACGCGGCGGAAGATGCTGACGTTACGCTCCAGCTGCATCTGAAGATGTGGCCGAAGCTACAGCAGCATGAAGGCCCGCTGAATATCTTCCAGCACATTGAAATGCCGCTGGTGCCGGTGCTGTCTCGCGTTGAGCGCAACGGCGTTAAAATCGACCCCGCCGTGCTGCATGCGCACTCGCAGGAAATTGCCCAGCGCTTGGTTGAGCTGGAGCAGCGGGCACATGAGATCGCCGGGGAAGCGTTTAATCTCTCCTCCACCAAACAGCTGCAAACGATCCTGTTTGAAAAACAGGGTATCAAGCCGCTGAAGAAGACGCCGGGCGGTGCGCCGTCCACCTCTGAAGAGGTGCTGGAAGAGCTGGCGCTCGATTATCCGTTGCCGAAAGTGATTCTGGAGTATCGTGGGCTGGCGAAGCTGAAGTCTACCTATACCGATAAGCTGCCGCTGATGATTAACCCCAAAACGGGCCGCGTGCATACCTCCTATCATCAGGCGGTCACCGCCACCGGCCGTTTGTCGTCTACCGATCCAAACCTGCAAAACATTCCGGTGCGTAACGAAGAAGGGCGCCGTATTCGCCAGGCGTTTATCGCGCCGGAAGATTACGTCATTGTCTCGGCGGACTACTCGCAAATCGAGCTGCGCATTATGGCGCACCTGTCGCGGGATAAAGGCCTGCTGACCGCCTTCGCGGAAGGGAAGGATATTCACCGGGCGACGGCGGCTGAAGTATTTGGCTTACCGCTGGACAGCGTCAGCAGCGAACAGCGGCGTAGTGCGAAAGCCATCAACTTCGGTCTGATCTACGGCATGAGCGCCTTTGGCCTGGCCCGCCAGCTGAACATCCCGCGTAAAGAAGCGCAGAAGTACATGGATCTTTATTTCGAGCGTTATCCGGGCGTCCTGGAGTATATGGAGCGCACCCGCGCACAGGCGAAGGAGCAAGGGTATGTTGAAACGCTGGATGGCCGTCGACTCTATTTGCCGGATATCAAGTCCAGCAATGGCGCTCGTCGTGCCGGCGCGGAGCGTGCGGCGATTAACGCCCCGATGCAGGGGACGGCTGCGGATATTATCAAGAGGGCGATGATTGCCGTTGATGAATGGCTGCGGAGCGAGAAACCTCGCGTGCGGATGATCATGCAGGTTCACGATGAACTGGTCTTTGAAGTGCATAAGGATGAGCTCGATGCCGTATCGAAAAAGATCCACGAACTGATGGAGAACAGCACTACGCTGGCGGTACCGTTGCTGGTGGAAGTGGGCAGCGGCGAAAACTGGGATCAAGCGCAC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,970 total letters Query= IDP96849.102 Length=3195 Score E Sequences producing significant alignments: (Bits) Value 0_wt 5032 0.0 2_07D_D1_IDP96849.102_T7term.seq 1786 0.0 1_01A_D1_IDP96849.102_T7short.seq 1714 0.0 Q F P S R N N F V * L * E G D I H M H H H H H H S S G V D Query_1 55 CAATTCCCCT-CTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCT 143 1 7 ..T.......T......-.N.....................................................----------------- 78 L W S H P Q F E K G T E N L Y F Q S N A M V Q I P E N P L I Query_1 144 GTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGGTTCAGATCCCAGAAAACCCTCTCATCCT 233 0 1 ................................ 32 1 79 -------------------------------........................................................... 137 L V D G S S Y L Y R A Y H A F P P L T N S A G E P T G A M Y Query_1 234 CGTTGACGGCTCCTCCTATCTTTATCGGGCGTATCATGCGTTTCCGCCGCTGACTAACAGCGCAGGGGAGCCCACCGGGGCTATGTACGG 323 0 33 .......................................................................................... 122 1 138 .......................................................................................... 227 G V L N M L R S L I L Q Y Q P T H A V V V F D A K G K T F R Query_1 324 CGTACTGAATATGCTGCGCAGTTTGATACTGCAGTATCAACCGACTCATGCCGTCGTGGTCTTTGATGCGAAAGGGAAAACCTTCCGTGA 413 0 123 .......................................................................................... 212 0 162 .................. 145 1 228 .......................................................................................... 317 1 267 .................. 250 D E L F E H Y K S H R P P M P D D L R A Q I E P L H K M V K Query_1 414 TGAATTGTTCGAGCATTACAAATCTCACCGCCCGCCGATGCCGGACGATCTGCGCGCGCAGATTGAGCCGTTGCATAAGATGGTGAAAGC 503 0 213 .......................................................................................... 302 0 275 ................ 260 1 318 .......................................................................................... 407 1 380 ................ 365 A M G L P L M A V P G V E A D D V I G T L A R E A E R A G R Query_1 504 GATGGGGCTGCCGCTGATGGCTGTCCCGGGCGTCGAAGCTGACGATGTGATTGGCACCCTGGCGCGGGAAGCGGAAAGGGCAGGTCGCCC 593 0 303 .......................................................................................... 392 0 1935 ............. 1947 2 990 ............. 978 1 408 .......................................................................................... 497 P V L I S T G D K D M A Q L V T P G I T L I N T M T N T I L Query_1 594 AGTCCTGATCAGCACCGGCGATAAAGACATGGCCCAGCTGGTGACGCCGGGCATCACCCTGATCAATACCATGACCAACACTATCCTCGG 683 0 393 .......................................................................................... 482 1 498 .......................................................................................... 587 G P D E V V T K Y G V P P E L I I D F L A L M G D S S D N I Query_1 684 ACCGGATGAGGTGGTTACTAAGTACGGCGTCCCGCCGGAGCTGATTATTGATTTCCTGGCGCTGATGGGCGACTCCTCGGATAACATTCC 773 0 483 .......................................................................................... 572 1 588 .......................................................................................... 677 P G V P G V G E K T A Q A L L Q G L G G L D T L Y A E P E K Query_1 774 CGGCGTGCCGGGCGTGGGCGAAAAGACCGCCCAGGCGCTGCTGCAAGGCCTCGGCGGCCTTGATACGCTGTATGCGGAACCGGAGAAGAT 863 0 573 .......................................................................................... 662 0 1134 ...........C.. 1147 1 678 .......................................................................................... 767 1 1051 ...A........... 1037 I A E L S F R G A K T M A A K L E Q N K D V A Y L S Y Q L A Query_1 864 CGCTGAACTGAGCTTCCGCGGCGCGAAAACGATGGCGGCGAAGCTCGAGCAGAACAAGGACGTGGCTTATCTCTCTTATCAGCTGGCGAC 953 0 663 .......................................................................................... 752 1 768 .......................................................................................... 857 T I K T D V E L E L T C E E L E V Q P P A A D D L L A L F R Query_1 954 CATCAAAACCGA-CGTCGAGCTGGAGCTCACCTGCGAAGAGCTGGAGGTGCAGCC-GCCGGCGGCAGACGATCTGCTGGCGCTGTTCAGA 1041 0 753 ............-..........................................-.................................. 840 0 815 ...-......... 804 1 858 ............C.T.......................N................G.................................. 947 Q Y E F K R W T T D V E A G K W L Q A K G G K P A A K P A Query_1 1042 CAG-TATGAATTTAAGCGCTGGACCACTGACGTTGAGGCCGGAAAATGGCTGCAGGCTAAGGGGGGCAAACCGGCTGCGAAACCTGCAGT 1130 0 841 ...-...................................................................................... 929 1 948 ...T.........-.N.........N...........................N....................N...N.........A. 1036 V P A A A A E A E E E V E A A T A L S A E H Y V T I L D Query_1 1131 GCC-TGCAGCAGCGGCAGAAGCGGAAGAA-GAGGTTG-AAGCCG-CTA-CTG-CAC-TTTCAGCAGAGCATTATGTCACTATCCTTGATG 1213 0 930 ...-.........................-.......-......-...-...-...-................................. 1012 1 1037 ...T.......T..CNNA.....A.....NN..T...A......N...C...C...T........A.ANN.A.....N... 1117 E A T L L T W I D K L K Q A P L F A F D T E T D S L D N I S Query_1 1214 AGGCTACGCTGCTCACCTGGATCGATAAGCTGAAGCAGGCGCCGCTCTTTGCCTTTGATACGGAAACCGACAGCCTGGATAACATCTCCG 1303 0 1013 .......................................................................................... 1102 A N M V G L S F A V E P G V A A Y V P V A H D Y L D A P D Q Query_1 1304 CCAACATGGTTGGTCTTTCATTCGCTGTTGAGCCGGGCGTGGCCGCCTATGTCCCGGTAGCGCATGATTACCTTGATGCGCCGGATCAGA 1393 0 1103 .......................................................................................... 1192 0 579 ...........G.. 592 1 684 ...........G.. 697 I P R E R V L T L L K P L L E D E K V L K V G Q N L K Y D R Query_1 1394 TCCCGCGCGAGCGCGTGCTGACGCTGCTTAAACCGCTGCTGGAGGATGAGAAGGTGCTCAAGGTCGGGCAAAATCTCAAATACGATCGCG 1483 0 1193 .......................................................................................... 1282 2 665 ........... 675 G I L A N Y D I E L R G I A F D T M L E S Y I L D S V A G R Query_1 1484 GCATTTTGGCTAATTACGACATTGAGCTGCGCGGGATTGCGTTCGATACCATGCTCGAGTCTTACATTCTCGACAGCGTGGCTGGCCGCC 1573 0 1283 .......................................................................................... 1372 H D M D S L S D R W L K H K T I T F E E I A G K G K N Q L T Query_1 1574 ACGATATGGACAGTCTTTCCGACCGCTGGCTGAAACATAAAACCATTACCTTCGAAGAGATCGCTGGCAAGGGTAAGAATCAGCTCACCT 1663 0 1373 .......................................................................................... 1462 F N Q I A L E E A G R Y A A E D A D V T L Q L H L K M W P K Query_1 1664 TTAACCAGATTGCGCTGGAAGAGGCTGGTCGCTACGCGGCGGAAGATGCTGACGTTACGCTCCAGCTGCATCTGAAGATGTGGCCGAAGC 1753 0 1463 .......................................................................................... 1552 L Q Q H E G P L N I F Q H I E M P L V P V L S R V E R N G V Query_1 1754 TACAGCAGCATGAAGGCCCGCTGAATATCTTCCAGCACATTGAAATGCCGCTGGTGCCGGTGCTGTCTCGCGTTGAGCGCAACGGCGTTA 1843 0 1553 .......................................................................................... 1642 0 1853 ........... 1843 K I D P A V L H A H S Q E I A Q R L V E L E Q R A H E I A G Query_1 1844 AAATCGACCCCGCCGTGCTGCATGCGCACTCGCAGGAAATTGCCCAGCGCTTGGTTGAGCTGGAGCAGCGGGCACATGAGATCGCCGGGG 1933 0 1643 .......................................................................................... 1732 E A F N L S S T K Q L Q T I L F E K Q G I K P L K K T P G G Query_1 1934 AAGCGTTTAATCTCTCCTCCACCAAACAGCTGCAAACGATCCTGTTTGAAAAACAGGGTATCAAGCCGCTGAAGAAGACGCCGGGCGGTG 2023 0 1733 .......................................................................................... 1822 A P S T S E E V L E E L A L D Y P L P K V I L E Y R G L A K Query_1 2024 CGCCGTCCACCTCTGAAGAGGTGCTGGAAGAGCTGGCGCTCGATTATCCGTTGCCGAAAGTGATTCTGGAGTATCGT-GGGCTGGCGAAG 2112 0 1823 .............................................................................-............ 1911 0 1590 ........... 1580 2 1034 .....NN............ 1016 L K S T Y T D K L P L M I N P K T G R V H T S Y H Q A V T Query_1 2113 CTGAAGTCTACCTATA-CCGAT-AAGCTGCCGCTGATGATTAACCCCAAAACGGGCCGCGTGCATACCTCCTATCATCAGGCGGTCACCG 2200 0 1912 ................-.....-................................................................... 1999 0 309 ............. 321 2 1015 .......N........N.....N................................................................... 926 1 414 ............. 426 A T G R L S S T D P N L Q N I P V R N E E G R R I R Q A F I Query_1 2201 CCACCGGCCGTTTGTCGTCTACCGATCCAAACCTGCAAAACATTCCGGTGCGTAACGAAGAAGGGCGCCGTATTCGCCAGGCGTTTATCG 2290 0 2000 .......................................................................................... 2089 2 925 .......................................................................................... 836 A P E D Y V I V S A D Y S Q I E L R I M A H L S R D K G L L Query_1 2291 CGCCGGAA-GATTACGTCATTGTCTCGGCGGACTACTCGCAAATCGAGCTGCGCATTATGGCGCACCTGTCGCGGGATAAAGGCCTGCTG 2379 0 2090 ........-................................................................................. 2178 2 835 ........A................................................................................. 746 T A F A E G K D I H R A T A A E V F G L P L D S V S S E Q Query_1 2380 ACCGCCTTCGCGGAA-GGGAAGGATATT-CACCGGGCGACGGCGGCTGAAGTATTTGGCTTACCGCTGGACAGCGTCAGCAGCGAACAGC 2467 0 2179 ...............-............-............................................................. 2266 2 745 ...............A............T............................................................. 656 R R S A K A I N F G L I Y G M S A F G L A R Q L N I P R K E Query_1 2468 GGCGTAGTGCGAAAGCCATCAACTTCGGTCTGATCTACGGCATGAGCGCCTTTGGCCTGGCCCGCCAGCTGAACATCCCGCGTAAAGAAG 2557 0 2267 .......................................................................................... 2356 2 655 .......................................................................................... 566 A Q K Y M D L Y F E R Y P G V L E Y M E R T R A Q A K E Q G Query_1 2558 CGCAGAAGTACATGGATCTTTATTTCGAGCGTTATCCGGGCGTCCTGGAGTATATGGAGCGCACCCGCGCACAGGCGAAGGAGCAAGGGT 2647 0 2357 .......................................................................................... 2446 2 565 .......................................................................................... 476 Y V E T L D G R R L Y L P D I K S S N G A R R A G A E R A A Query_1 2648 ATGTTGAAACGCTGGATGGCCGTCGACTCTATTTGCCGGATATCAAGTCCAGCAATGGCGCTCGTCGTGCCGGCGCGGAGCGTGCGGCGA 2737 0 2447 .......................................................................................... 2536 2 475 .......................................................................................... 386 I N A P M Q G T A A D I I K R A M I A V D E W L R S E K P R Query_1 2738 TTAACGCCCCGATGCAGGGGACGGCTGCGGATATTATCAAGAGGGCGATGATTGCCGTTGATGAATGGCTGCGGAGCGAGAAACCTCGCG 2827 0 2537 .......................................................................................... 2626 2 385 .......................................................................................... 296 V R M I M Q V H D E L V F E V H K D E L D A V S K K I H E L Query_1 2828 TGCGGATGATCATGCAGGTTCACGATGAACTGGTCTTTGAAGTGCATAAGGATGAGCTCGATGCCGTATCGAAAAAGATCCACGAACTGA 2917 0 2627 .......................................................................................... 2716 2 295 .......................................................................................... 206 M E N S T T L A V P L L V E V G S G E N W D Q A H * H W K W Query_1 2918 TGGAGAACAGCACTACGCTGGCGGTACCGTTGCTGGTGGAAGTGGGCAGCGGCGAAAACTGGGATCAAGCGCACTAACATTGGAAGTGGA 3007 0 2717 .......................................................................... 2790 2 205 .......................................................................................... 116 I T D P N S S S V D K L A A A L E H H H H H H * D P A A N K Query_1 3008 TAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAG 3097 2 115 .......................................................................................... 26 A R K E A E L A A A T A E Q * L A * P L G A S K R V L R G F Query_1 3098 CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTT 3187 2 25 ............ 14 1 1104 ... 1102 L L K Query_1 3188 TGCTGAAA 3195 1 1101 ........ 1094 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 15618132 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 4,970 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96851.102 (IDP96851.1-331.F..pMCSG53)
MASLKDVAKLANVSLMTVSRALNTPERLKPETLARVQAAIAETNYVPDLSAKKIRGARATPSTIGVLALDTVTTPFSVEITLSIEETARAHGWNSFVVNMFSDDRPEAVVDLLLSHRPDGIIFTTMGLRQVPLPEKLLTLPCVLANCESLSQPVASYIPDDEQGQYDAVKALLAAGYRRPLCLHLPASQPATIRRRRGLERACREAGIEPDHLSHSYMGQGDEHYHDIPAVVLAHIREGKPGFDSVICGNDRIAFMVYQTLLGQGLRIPQDVAVVGYDNMVGIGDLFLPPLSTVQLPHYDIGRLSALHIIHGDNHRETRKVASPWLPRASH
ATGGCGTCCCTGAAAGATGTAGCAAAACTGGCGAATGTATCTCTGATGACCGTCTCACGGGCGCTGAACACCCCGGAGCGGCTGAAGCCTGAGACGCTGGCCCGCGTGCAGGCCGCCATCGCCGAAACCAACTACGTCCCCGACCTGTCGGCGAAGAAGATCCGCGGGGCGCGCGCAACCCCCAGCACCATCGGCGTGCTGGCGCTGGATACCGTCACCACGCCCTTTTCGGTGGAGATCACCCTCTCGATCGAAGAGACGGCCCGCGCCCACGGCTGGAACAGCTTTGTGGTCAATATGTTTTCTGACGATCGTCCGGAAGCGGTGGTCGACTTGCTCCTCTCCCACCGTCCGGACGGCATTATCTTTACCACCATGGGCCTGCGCCAGGTACCGCTGCCGGAAAAACTGCTGACTCTGCCCTGCGTGCTGGCCAACTGCGAAAGCCTCAGCCAGCCGGTGGCCAGCTATATCCCGGACGATGAACAGGGGCAGTACGACGCGGTGAAAGCCCTGCTTGCCGCTGGCTATCGTCGTCCGCTGTGCCTGCACTTACCCGCCAGCCAGCCGGCGACGATCCGCCGCCGCCGGGGGCTGGAGCGCGCCTGCCGGGAGGCCGGTATCGAACCGGACCATCTGAGCCATTCGTATATGGGCCAGGGCGATGAGCATTACCACGATATCCCGGCGGTGGTGCTGGCGCATATCCGCGAGGGTAAACCTGGCTTTGACTCAGTGATCTGCGGCAACGATCGCATCGCGTTTATGGTCTATCAGACGCTGCTGGGCCAGGGGTTACGCATTCCACAGGACGTGGCGGTAGTGGGATACGACAACATGGTGGGCATTGGCGATCTGTTTCTACCGCCGCTCTCCACAGTGCAACTGCCGCATTATGACATCGGGCGCCTGAGCGCCCTGCATATCATTCACGGCGACAACCATCGGGAGACGCGCAAAGTCGCCAGCCCGTGGTTGCCGCGCGCCTCACAT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,461 total letters Query= IDP96851.102 Length=1398 Score E Sequences producing significant alignments: (Bits) Value 2_07F_F1_IDP96851.102_T7term.seq 1840 0.0 0_wt 1792 0.0 1_01F_F1_IDP96851.102_T7S.seq 1761 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 60 CCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTC 149 1 24 ...........-.......................................................----------------------- 89 S H P Q F E K G T E N L Y F Q S N A M A S L K D V A K L A N Query_1 150 TCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGGCGTCCCTGAAAGA-TGTAGCAAAACTGGCG-AAT 237 2 1129 ..N......N.........-......G... 1101 0 1 .................-................-... 36 1 90 -------------------------............................................-................-... 152 V S L M T V S R A L N T P E R L K P E T L A R V Q A A Query_1 238 GTATC--TCTGATGACCGT-CTCA-CGGGCGCTGAACACCCCGGA-GC-GGCTGAAGCCTGAGACGCTG-GCCC-GCGTGCAGGCCGCCA 319 2 1100 ....ACT.NNA....T...T....A....................A..A..N.................A....N........C...... 1011 0 37 .....--............-....-....................-..-....................-....-............... 118 1 153 .....--............-....-....................-..-....................-....-............... 234 I A E T N Y V P D L S A K K I R G A R A T P S T I G V L A Query_1 320 TCGCCGAAACCAACTACGTCCCCGA-CCTGTCGGCGAAGAAGATCCGCGGGGCGCGCGCAACCCCC-AGCACCATCGGCGTGCTGGCGCT 407 2 1010 .........................A........................................C....................... 921 0 119 .........................-........................................-....................... 206 1 235 .........................-........................................-....................... 322 L D T V T T P F S V E I T L S I E E T A R A H G W N S F V V Query_1 408 GGATACCGTCACCACGCCCTTTTCGGTGGAGATCA-CCCTCTCGATCGAAGAGACGGCCCGCGCCCACGGCTGGAACAGCTTTGTGGTCA 496 2 920 ......................-............A..............................................-....... 833 0 207 ...................................-...................................................... 295 1 323 ...................................-...................................................... 411 N M F S D D R P E A V V D L L L S H R P D G I I F T T M G L Query_1 497 ATATGTTTTCTGACGATCGTCCGGAAGCGG-TGGTCGACTTGCTCCTCTCCCACCGTCCGGACGGCATTATCTTTACCACCATGGGCCTG 585 2 832 ..............................G........................................................... 743 2 768 ............ 779 0 296 ..............................-........................................................... 384 0 359 ............ 348 1 412 ..............................-........................................................... 500 1 475 ............ 464 R Q V P L P E K L L T L P C V L A N C E S L S Q P V A S Y I Query_1 586 CGCCAGGTACCGCTGCCGGAAAAACTGCTGACTCTGCCCTGCGTGCTGGCCAACTGCGAAAGCCTCAGCCAGCCGGTGGCCAGCTATATC 675 2 742 .......................................................................................... 653 2 566 ........... 556 0 385 .......................................................................................... 474 0 561 ........... 571 1 501 .......................................................................................... 590 1 677 ........... 687 P D D E Q G Q Y D A V K A L L A A G Y R R P L C L H L P A S Query_1 676 CCGGACGATGAACAGGGGCAGTACGACGCGGTGAAAGCCCTGCTTGCCGCTGGCTATCGTCGTCCGCTGTGCCTGCACTTACCCGCCAGC 765 2 652 .......................................................................................... 563 2 677 .... 674 0 475 .......................................................................................... 564 0 450 .... 453 1 591 .......................................................................................... 680 1 566 .... 569 Q P A T I R R R R G L E R A C R E A G I E P D H L S H S Y Query_1 766 CAGCCGGCGACGATCCGCCGCCGCCGGGGGCTGGAGCGCGCCTGCCGGGAGGCCGGTATCGAACCGGACCA-TCTGAGCCATTCGTATAT 854 2 562 .......................................................................-.................. 474 2 673 ....... 667 0 565 .......................................................................-.................. 653 0 454 ....... 460 1 681 .......................................................................T............N..... 770 1 570 ....... 576 M G Q G D E H Y H D I P A V V L A H I R E G K P G F D S V I Query_1 855 GGGCCAGGGCGATGAGCATTACCACGATATCCCGGCGGTGGTGCTGGCGCATATCCGCGAGGGTAAACCTGGCTTTGACTCAGTGATCTG 944 2 473 .......................................................................................... 384 0 654 .......................................................................................... 743 1 771 ..................................................................N....................... 860 C G N D R I A F M V Y Q T L L G Q G L R I P Q D V A V V G Y Query_1 945 CGGCAACGATCGCATCGCGTTTATGGTCTATCAGACGCTGCTGGGCCAGGGGTTACGCATTCCACAGGACGTGGCGGTAGTGGGATACGA 1034 2 383 .......................................................................................... 294 0 744 .......................................................................................... 833 1 861 ..................................................................A......C................ 950 D N M V G I G D L F L P P L S T V Q L P H Y D I G R L S A L Query_1 1035 CAACATGGTGGGCATTGGCGATCTGTTTCTACCGCCGCTCTCCACAGTGCAACTGCCGCA-TTATGACATCGGGCGCCTGAGCGCCCTGC 1123 2 293 ............................................................-............................. 205 0 834 ............................................................-............................. 922 1 951 .........................-...............-.N................T................T............ 1038 H I I H G D N H R E T R K V A S P W L P R A S H * H W Query_1 1124 A-TATCATTC-ACGGCG-AC-AACCATCGGGAGACGCGCAAAGT-CGCCAGCCC-GTGGTTGCCGCGC-GCCT-CACATTAACATTGGAA 1205 2 204 .-........-......-..-.......................-.........-.............-....-................ 123 0 923 .-........-......-..-.......................-.........-.............-....-..... 993 1 1039 .T........NN.....N..A.................N.....N.........G........N...NG....N.NN....C.......N 1128 K W I T D P N S S S V D K L A A A L E H H H H H H * D P Query_1 1206 GTGG-ATAACGG-ATCCGA-ATT--CGAGC-TCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGC 1289 2 122 ....-.......-......-...--.....-........................................................... 39 1 1129 ..N.A.N....NA.NN...T...TCN....T............. 1172 A A N K A R K Query_1 1290 TGCTAACAAAGCCCGAAAGGA 1310 2 38 ..................A.. 18 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4716766 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 3,461 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96852.102 (IDP96852.1-442.F..pMCSG53)
MKERRTELVEGFRHSVPYINAHRGKTFVIMLGGEAIEHENFSNIVNDIGLLHSLGIRLVVVYGARPQIDTNLAEHHHEPVYHKQTRVTDAKTLELVKQAAGMLQLEITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEEAIHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVYNQAGEIVSELFPNEAQARVEELEADEDYNSGTVRFLRGAVKACRSGVRRCHLISYQENGALLQELFSRDGIGTQIVMESAEQIRRATINDIGGILELISPLEQQGILVRRSREQLEMEIDKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQARQMGLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQMYNYQRRSKVLMADLA
GTGAAGGAACGTCGCACTGAACTGGTAGAAGGATTCCGCCATTCTGTTCCCTATATTAATGCCCATCGGGGAAAAACGTTTGTCATCATGCTGGGCGGCGAAGCCATTGAGCATGAAAATTTCTCAAATATTGTCAATGACATAGGATTATTGCATAGCCTGGGCATTCGCCTGGTGGTGGTCTATGGCGCCCGCCCGCAGATTGACACTAACCTCGCCGAGCACCATCACGAGCCGGTCTATCACAAGCAGACGCGAGTGACTGACGCCAAAACGCTGGAGCTGGTTAAGCAGGCGGCGGGGATGCTGCAGCTGGAGATCACCGCCCGTCTGTCGATGAGCCTCAACAACACCCCGCTGCAGGGGGCCCATATCAACGTTGTCAGCGGCAACTTTATCATTGCCCAGCCGCTGGGCGTCGATGACGGCGTCGATTATTGCCACAGCGGACGCATTCGCCGCATTGACGAAGAGGCGATTCATCGTCAGCTCGACAGCGGCGCCATTGTCCTGATGGGGCCGGTCGCGGTGTCGGTCACCGGCGAGAGCTTTAACCTGACTTCGGAAGAGATCGCTACCCAGCTGGCCATCAAGCTGAAGGCGGAGAAAATGATCGGCTTCTGTTCGTCGCAAGGGGTTTACAACCAGGCCGGGGAAATTGTCTCCGAACTGTTCCCTAATGAGGCGCAAGCCCGCGTCGAAGAGCTGGAAGCAGACGAAGACTATAACTCCGGCACCGTGCGCTTCCTGCGCGGGGCGGTGAAGGCCTGCCGCAGCGGCGTACGGCGCTGCCACCTGATCAGCTATCAGGAAAATGGCGCGCTGCTGCAGGAGCTGTTTTCACGCGACGGCATCGGCACCCAGATCGTCATGGAGAGCGCGGAGCAGATCCGTCGGGCGACCATCAATGATATCGGCGGTATTCTTGAGCTGATAAGCCCGCTCGAGCAGCAGGGCATTCTGGTACGCCGTTCCCGCGAACAGCTGGAGATGGAGATCGACAAATTCACCATTATTCAGCGCGATAACACCACCATCGCCTGCGCCGCCCTCTATCCTTTCCCGGAAGAGAAGATCGGCGAAATGGCCTGCGTGGCGGTGCATCCGGATTACCGGAGCTCGTCGCGGGGAGAGGTGCTGCTGGAGCGTATTGCCGCTCAGGCCAGGCAGATGGGACTGAGCAAGCTGTTCGTGTTAACCACCCGCAGCATCCACTGGTTCCAGGAGCGCGGCTTCACGCCGGTGGATATCGACCTGCTGCCGGAGAGTAAAAAGCAGATGTATAACTATCAGCGACGGTCGAAGGTGCTGATGGCCGACCTCGCT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,861 total letters Query= IDP96852.102 Length=1731 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2392 0.0 2_07E_E1_IDP96852.102_T7term.seq 1972 0.0 1_01E_E1_IDP96852.102_T7S.seq 1786 0.0 Q F P S R N N F V * L * E G D I H M H H H H H H S S G V D Query_1 55 CAATTCCCCTC-TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCT 143 1 16 ..T........C.............................................................----------------- 88 L W S H P Q F E K G T E N L Y F Q S N A V K E R R T E L V E Query_1 144 GTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGTGAAGGAACGTCGCACTGAACTGGTAGAAGG 233 0 1 ................................ 32 1 89 -------------------------------........................................................... 147 G F R H S V P Y I N A H R G K T F V I M L G G E A I E H E N Query_1 234 ATTCCGCCATTCTGTTCCCTATATTAATGCCCATCGGGGAAAAACGTTTGTCATCATGCTGGGCGGCGAAGCCATTGAGCATGAAAATTT 323 0 33 .......................................................................................... 122 1 148 .......................................................................................... 237 F S N I V N D I G L L H S L G I R L V V V Y G A R P Q I D T Query_1 324 CTCAAATATTGTCAATGACATAGGATTATTGCATAGCCTGGGCATTCGCCTGGTGGTGGTCTATGGCGCCCGCCCGCAGATTGACACTAA 413 0 123 .......................................................................................... 212 1 238 .......................................................................................... 327 N L A E H H H E P V Y H K Q T R V T D A K T L E L V K Q A A Query_1 414 CCTCGCCGAGCACCATCACGAGCCGGTCTATCACAAGCAGACGCGAGTGACTGACGCCAAAACGCTGGAGCTGGTTAAGCAGGCGGCGGG 503 0 213 .......................................................................................... 302 2 1171 ....NNN...... 1159 1 328 .......................................................................................... 417 G M L Q L E I T A R L S M S L N N T P L Q G A H I N V V S G Query_1 504 GATGCTGCAGCTGGA-GATCACCGCCCGT-CTGTCGATGAGCCTCAACAACACCCCGCTGCAGGGGGCCCATATCAACGTTGTCAGCGGC 591 0 303 ...............-.............-............................................................ 390 0 982 ........-... 992 2 1158 ....N.........NN...NN..N.....T............T........N...-.NN............NN.......GN........ 1070 2 475 ........-... 465 1 418 ...............-.............-............................................................ 505 1 1107 ........-... 1117 N F I I A Q P L G V D D G V D Y C H S G R I R R I D E E A Query_1 592 AACTTTATCATTGCCCAG-CCGCTGGGCGTCGATGACGGCGTCGATTATTGCCACAGCGGACGCATTCGCCGCATTGACGAAGAGGCGAT 680 0 391 ..................-....................................................................... 479 2 1069 ..................G.NN.............................N...................................... 980 1 506 ..................-....................................................................... 594 I H R Q L D S G A I V L M G P V A V S V T G E S F N L T S E Query_1 681 TCATCGTCAGCTCGACAGCGGCGCCATTGTCCTGATGGGGCC-GGTCGCGGTGTCGGTCACCGGCGAGAGCTTTAACCTGACTTCGG-AA 768 0 480 ..........................................-............................................-.. 567 2 979 ..........................................C............................................G.. 890 1 595 ..........................................-............................................-.. 682 E I A T Q L A I K L K A E K M I G F C S S Q G V Y N Q A G E Query_1 769 GAGATCGCTACCCAGCTGGCCATCAAGCTGAAGGCGGAGAAAATGATCGGCTTCTGTTCGTCGCAAGGGGTTTACAACCAGGCCGGGGAA 858 0 568 .......................................................................................... 657 2 889 .......................................................................................... 800 1 683 .......................................................................................... 772 I V S E L F P N E A Q A R V E E L E A D E D Y N S G T V R F Query_1 859 ATTGTCTCCGAACTGTTCCCTAATGAGGCGCAAGCCCGCGTCGAAGAGCTGGAAGCAGACGAAGACTATAACTCCGGCACCGTGCGCTTC 948 0 658 .......................................................................................... 747 2 799 .......................................................................................... 710 1 773 .......................................................................................... 862 L R G A V K A C R S G V R R C H L I S Y Q E N G A L L Q E L Query_1 949 CTGCGCGGGGCGGTGAAGGCCTGCCGCAGCGGCGTACGGCGCTGCCACCTGATCAGCTATCAGGAAAATGGCGCGCTGCTGCAGGAGCTG 1038 0 748 .......................................................................................... 837 2 709 .......................................................................................... 620 1 863 ..................................................................................NN...... 952 F S R D G I G T Q I V M E S A E Q I R R A T I N D I G G Query_1 1039 TTTT-CACGCGACGGCATCGGCACCCAGATCGTCATGGAGAGCGC-GGAGCAGAT-CCGTCGGGCG-ACCATCAATG-ATATCGGC-GGT 1122 0 838 ....-........................................-.........-..........-..........-........-... 921 2 619 ....-........................................-.........-..........-..........-........-... 536 1 953 ....N..................................N.....G.........N..........A..........A........N... 1042 I L E L I S P L E Q Q G I L V R R S R E Q L E M E I D K Query_1 1123 ATT-CTTGAGCTGATAAGCCCGCTCGAGCAGCAGGGCATT-CTGGTACG-CCGTTCCCG-CGAACAGCTGGAGATGGAG-ATCGACAAAT 1207 0 922 ...-....................................-........-.........-...................-.......... 1006 0 310 ........... 320 2 535 ...-....................................-........-.........-...................-.......... 451 1 1043 ...C...........N....................NN..C.N......C..NN.....NN..................A.....AC..N 1132 1 425 ........... 435 F T I I Q R D N T T I A C A A L Y P F P E E K I G E M A C V Query_1 1208 TCA-CCATTATTCAGCGCGATAACACCACCATCGCCTGCGCCGCCCTCTATCCTTTCCCGGAAGAGAAGATCGGCGAAATGGCCTGCGTG 1296 0 1007 ...-...................................................................................... 1095 2 450 ...-...................................................................................... 362 1 1133 N..N.......... 1146 A V H P D Y R S S S R G E V L L E R I A A Q A R Q M G L S K Query_1 1297 GCGGTGCATCCGGATTACCGGAGCTCGTCGCGGGGAGAGGTGCTGCTGGAGCGTATTGCCGCTCAGGCCAGGCAGATGGGACTGAGCAAG 1386 0 1096 .......................................................................................... 1185 2 361 .......................................................................................... 272 L F V L T T R S I H W F Q E R G F T P V D I D L L P E S K K Query_1 1387 CTGTTCGTGTTAACCACCCGCAGCATCCACTGGTTCCAGGAGCGCGGCTTCACGCCGGTGGATATCGACCTGCTGCCGGAGAGTAAAAAG 1476 0 1186 .......................................................................................... 1275 2 271 .......................................................................................... 182 Q M Y N Y Q R R S K V L M A D L A * H W K W I T D P N S S S Query_1 1477 CAGATGTATAACTATCAGCGACGGTCGAAGGTGCTGATGGCCGACCTCGCTTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCC 1566 0 1276 ................................................... 1326 2 181 .......................................................................................... 92 V D K L A A A L E H H H H H H * D P A A N K A R K E A Query_1 1567 GTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCT 1647 2 91 ...........................................................................A..... 11 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 6539295 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 3,861 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96853.102 (IDP96853.1-506.F..pMCSG53)
MVSNNSERSGEYLLEMSNINKSFPGVKALDNVNLKVRPHSIHALMGENGAGKSTLLKCLFGIYQKDSGSILFQGKEIDFHSAKEALENGISMVHQELNLVLQRSVMDNMWLGRYPTKGMFVDQDKMYRDTKAIFDELDIDIDPRARVGTLSVSQMQMIEIAKAFSYDAKIVIMDEPTSSLTEKEVNHLFKIIRKLKDRGCGIVYISHKMEEIFQLCDEITILRDGQWIATQPLEGLDMDKIIAMMVGRSLNQRFPDRENTPGEVILQVRNLTSLRQPSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKASGTITLHGKKINNHSANEAINHGFALVTEERRSTGIYAYLDIGFNSLISNIKKYKNSIGLLDNSRMKSDTQWVIDSMRVKTPGQHTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIAELAKKDKGIIIISSEMPELLGITDRILVMSNGLVAGIVETKTTTQNEILRLASLHL
ATGGTCAGCAATAATAGCGAACGGTCAGGCGAATATTTGTTGGAGATGAGCAACATCAACAAATCGTTTCCTGGCGTCAAGGCTCTTGATAATGTCAACCTGAAGGTTCGTCCGCACTCCATTCATGCGTTAATGGGTGAGAACGGCGCCGGTAAATCGACATTATTAAAATGCCTTTTTGGGATCTATCAAAAAGATTCCGGTAGCATTCTTTTTCAGGGAAAAGAGATCGATTTCCATTCGGCGAAAGAAGCCCTGGAAAACGGTATTTCGATGGTGCATCAGGAATTAAACCTGGTATTACAGCGTTCGGTCATGGATAACATGTGGCTCGGGCGCTATCCAACTAAAGGCATGTTTGTCGATCAGGACAAAATGTACCGGGATACCAAGGCCATTTTTGATGAGCTGGATATTGATATCGATCCGCGCGCCCGCGTCGGTACCTTATCGGTATCTCAGATGCAGATGATTGAGATCGCGAAGGCGTTTTCCTATGACGCCAAAATCGTCATCATGGATGAGCCGACGTCATCGCTGACGGAAAAAGAGGTCAACCATCTGTTTAAGATCATCCGTAAGCTGAAAGATCGCGGCTGCGGTATCGTCTATATCTCGCATAAAATGGAAGAAATTTTCCAGTTGTGCGATGAAATAACCATCCTGCGCGACGGCCAGTGGATCGCCACCCAGCCGCTGGAAGGGCTGGATATGGATAAGATCATCGCCATGATGGTCGGGCGTTCGCTTAACCAGCGTTTTCCGGATCGCGAAAACACTCCGGGCGAAGTGATCCTCCAGGTGCGCAATCTCACCTCGCTGCGCCAGCCCTCCATTCGCGATGTCTCCTTTGACCTGCATAAGGGCGAAATTCTCGGTATCGCCGGCCTGGTGGGCGCTAAACGCACCGACATCGTGGAGACCCTGTTCGGCATCCGTGAGAAGGCCAGCGGCACCATCACGCTGCACGGCAAGAAGATCAACAACCACAGCGCCAACGAGGCGATTAACCACGGTTTTGCGCTGGTGACCGAGGAGCGGCGCTCTACCGGGATTTACGCCTATCTGGATATCGGATTTAACTCGCTGATCTCCAATATTAAGAAATATAAAAACAGCATCGGCTTGCTGGATAACTCGCGAATGAAGAGCGATACCCAATGGGTTATTGACTCGATGCGCGTCAAAACCCCCGGTCAGCATACGCAGATTGGCTCGCTTTCCGGTGGTAACCAGCAAAAGGTCATTATAGGCCGCTGGCTGCTGACCCAGCCGGAGATCCTGATGCTGGATGAGCCAACGCGCGGTATCGACGTCGGCGCCAAGTTTGAAATCTATCAGCTGATTGCCGAACTGGCTAAAAAAGACAAAGGGATCATTATTATTTCTTCCGAAATGCCGGAACTGCTGGGGATTACCGACCGCATTCTGGTGATGAGCAACGGCCTGGTCGCGGGCATTGTTGAGACTAAAACCACTACGCAAAACGAAATACTGCGTCTTGCGTCATTGCACCTT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,047 total letters Query= IDP96853.102 Length=1923 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2738 0.0 2_07H_H1_IDP96853.102_T7term.seq 1957 0.0 1_01H_H1_IDP96853.102_T7S.seq 1865 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 60 CCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTC 149 1 20 ...................................................................----------------------- 86 S H P Q F E K G T E N L Y F Q S N A M V S N N S E R S G E Y Query_1 150 TCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGGTCAGCAATAATAGCGAACGGTCAGGCGAATATTT 239 0 1 ...................................... 38 1 87 -------------------------................................................................. 151 L L E M S N I N K S F P G V K A L D N V N L K V R P H S I H Query_1 240 GTTGGAGATGAGCAACATCAACAAATCGTTTCCTGGCGTCAAGGCTCTTGATAATGTCAACCTGAAGGTTCGTCCGCACTCCATTCATGC 329 0 39 .......................................................................................... 128 1 152 .......................................................................................... 241 A L M G E N G A G K S T L L K C L F G I Y Q K D S G S I L F Query_1 330 GTTAATGGGTGAGAACGGCGCCGGTAAATCGACATTATTAAAATGCCTTTTTGGGATCTATCAAAAAGATTCCGGTAGCATTCTTTTTCA 419 0 129 .......................................................................................... 218 1 242 .......................................................................................... 331 Q G K E I D F H S A K E A L E N G I S M V H Q E L N L V L Q Query_1 420 GGGAAAAGAGATCGATTTCCATTCGGCGAAAGAAGCCCTGGAAAACGGTATTTCGATGGTGCATCAGGAATTAAACCTGGTATTACAGCG 509 0 219 .......................................................................................... 308 1 332 .......................................................................................... 421 R S V M D N M W L G R Y P T K G M F V D Q D K M Y R D T K A Query_1 510 TTCGGTCATGGATAACATGTGGCTCGGGCGCTATCCAACTAAAGGCATGTTTGTCGATCAGGACAAAATGTACCGGGATACCAAGGCCAT 599 0 309 .......................................................................................... 398 1 422 .......................................................................................... 511 I F D E L D I D I D P R A R V G T L S V S Q M Q M I E I A K Query_1 600 TTTTGATGAGCTGGATATTGATATCGATCCGCGCGCCCGCGTCGGTACCTTATCGGTATCTCAGA-TGCAGATGATTGAGATCGCGAAGG 688 0 399 .................................................................-........................ 487 2 1202 ......A.NN.....A..................NN.GN. 1163 1 512 .................................................................-........................ 600 A F S Y D A K I V I M D E P T S S L T E K E V N H L F K I Query_1 689 CGTTTTCCTATGACGCCAAAATCGTCATCATGGATGAGCCGACGTCATCGCTGACGGAAAAAGAGGTCAACCAT-CTGTTTAAG-ATCAT 776 0 488 ..........................................................................-.........-..... 575 2 1162 NTNN..---.N.........-...........AT.....-...............NA..NN.NN.-...N....T.....N...N..... 1079 1 601 ..........................................................................-.........-..... 688 I R K L K D R G C G I V Y I S H K M E E I F Q L C D E I T I Query_1 777 CCGTAAGCTGAAAGATCGCGGCTGCGGT-ATCGTCTATAT-CTCGCATAAAATGGAAGAAATTTTCCAGTTGTGCGATGAAATAACCATC 864 0 576 ............................-...........-................................................. 663 2 1078 ..................N.........N...........T...........................................C..... 989 1 689 ............................-...........-................................................. 776 L R D G Q W I A T Q P L E G L D M D K I I A M M V G R S L Query_1 865 CTGCGCGACGG-CCAGTGGATCGCCACCCAGCCGCTGGAAGGGCTGGATATGGATAAGATCATCGCCATGATGGTCGGGCGTTCGCTT-A 952 0 664 ...........-............................................................................-. 751 2 988 T..........A............................................................................-. 900 1 777 ...........-............................................................................N. 865 N Q R F P D R E N T P G E V I L Q V R N L T S L R Q P S I R Query_1 953 ACCAGCGTTTTCCGGATCGCGAAAACACTCCGGGCGAAGTGATCCTCCAGGTGCGCAATCTCACCTCGCTGCGCCAGCCCTCCATTCGCG 1042 0 752 .......................................................................................... 841 2 899 .......................................................................................... 810 1 866 N......................................................................................... 955 D V S F D L H K G E I L G I A G L V G A K R T D I V E T L Query_1 1043 ATGTCTCCTTTGACCTGCATAAGGGCGAAATTCTCGGTATCGCCGGCCTGGT-GGGCGCTAAA-CGCACCGACATCGTGGAGA-CCCTGT 1129 0 842 ....................................................-..........-...................-...... 928 2 809 ....................................................-..........A...................-...... 722 1 956 ....................................................G..........-..............-....C...... 1043 F G I R E K A S G T I T L H G K K I N N H S A N E A I N H Query_1 1130 TCGGCATCCGTGAGAAGGCCAGCGGCACCATCACGC-TGCACGGCAAGAAGA-TCAACAACCACAGCGCCAAC-GAGGCGATTAACCAC- 1215 0 929 ....................................-...............-....................-...............- 1014 2 721 ....................................-...............-....................-...............- 636 1 1044 .............N.....N................T...N...........T...N..T..........N..NN........T.A...G 1133 G F A L V T E E R R S T G I Y A Y L D I G F N S L I S N Query_1 1216 GGTTTTGCGCTGGTGACCGAGG-AGCGGCGCTCT--ACCGGGATTTACG-CCTA-TCTGGA-TATCGGATTTAACTCGCTGATCTCCAAT 1299 0 1015 ......................-...........--.............-....-......-............................ 1098 2 635 ......................-...........--.............-....-......-............................ 552 1 1134 ............TGN....N..A.......N...TA......GN.....C....NN.....N....... 1202 I K K Y K N S I G L L D N S R M K S D T Q W V I D S M R V K Query_1 1300 ATTAAGAAATATAAAAACAGCATCGGCTTGCTGGATAACTCGCGAATGAAGAGCGATACCCAATGGGTTATTGACTCGATGCGCGTCAAA 1389 0 1099 .......................................................................................... 1188 2 551 .......................................................................................... 462 T P G Q H T Q I G S L S G G N Q Q K V I I G R W L L T Q P E Query_1 1390 ACCCCCGGTCAGCATACGCAGATTGGCTCGCTTTCCGGTGGTAACCAGCAAAAGGTCATTATAGGCCGCTGGCTGCTGACCCAGCCGGAG 1479 0 1189 .......................................................................................... 1278 2 461 .......................................................................................... 372 I L M L D E P T R G I D V G A K F E I Y Q L I A E L A K K D Query_1 1480 ATCCTGATGCTGGATGAGCCAACGCGCGGTATCGACGTCGGCGCCAAGTTTGAAATCTATCAGCTGATTGCCGAACTGGCTAAAAAAGAC 1569 0 1279 .......................................................................................... 1368 2 371 .......................................................................................... 282 K G I I I I S S E M P E L L G I T D R I L V M S N G L V A G Query_1 1570 AAAGGGATCATTATTATTTCTTCCGAAATGCCGGAACTGCTGGGGATTACCGACCGCATTCTGGTGATGAGCAACGGCCTGGTCGCGGGC 1659 0 1369 .......................................................................................... 1458 2 281 .......................................................................................... 192 I V E T K T T T Q N E I L R L A S L H L * H W K W I T D P N Query_1 1660 ATTGTTGAGACTAAAACCACTACGCAAAACGAAATACTGCGTCTTGCGTCATTGCACCTTTAACATTGGAAGTGGATAACGGATCCGAAT 1749 0 1459 ............................................................ 1518 2 191 .......................................................................................... 102 S S S V D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 1750 TCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAG 1837 2 101 ...................................................................................A.... 14 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 7616376 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 4,047 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96858.102 (IDP96858.1-331.F..pMCSG53)
MKQTVYTASPESQQIHVWSLEADGKLTLVQVVDAPGQVQPMVVSPNKEFLYVGVRPEFRVLAYRITPDNGALTFAGEAALPGSPTHISTDRHGRFVFSASYNQGCVSVTPLHDGLPGETITVVEGLEGCHSANISPDNRTLWVPALKQDRICLFTLSDDGFLSAQEPAEVTTVEGAGPRHMVFHPNQQYGYCVNELNSSIDVWELKDPKGNIECVQTLDMMPPDFSGVRWAADIHITPDGRHLYACDRTASIITVFSVSEDGSVLAVEGYQPTETQPRGFNLDHSGKYLIAAGQKSHHIAVYDIVGEQGLLQEKGRYAVGQGPMWVVVNAH
ATGAAACAAACCGTTTATACCGCCAGCCCGGAAAGCCAACAAATCCATGTCTGGAGTCTTGAAGCGGACGGCAAACTGACGCTGGTGCAGGTGGTTGATGCCCCAGGGCAGGTCCAGCCGATGGTGGTCAGCCCCAACAAGGAATTTCTGTACGTTGGCGTTCGTCCGGAGTTTCGCGTGCTGGCCTATCGCATCACGCCGGATAACGGGGCGTTAACCTTTGCGGGCGAAGCTGCGCTGCCGGGCAGCCCGACCCATATCTCTACCGATCGTCATGGCCGCTTTGTCTTCAGCGCCTCCTACAATCAGGGCTGTGTCAGCGTGACCCCGCTGCACGACGGCCTGCCGGGCGAAACCATCACCGTGGTGGAAGGGCTGGAAGGCTGCCACTCGGCCAATATCTCCCCGGATAATCGCACCCTGTGGGTGCCGGCGCTGAAGCAGGATCGCATTTGCCTGTTTACCCTCAGCGACGATGGTTTCCTTTCGGCACAGGAGCCCGCGGAAGTGACGACCGTCGAGGGCGCCGGTCCGCGTCACATGGTATTCCATCCCAACCAGCAGTATGGTTACTGCGTCAATGAGCTAAACAGCTCGATTGACGTCTGGGAGCTGAAAGATCCTAAGGGCAACATCGAATGTGTACAGACCCTGGATATGATGCCGCCTGATTTTAGCGGCGTGCGCTGGGCGGCGGATATTCATATCACCCCGGATGGCCGTCATCTTTACGCCTGCGATCGTACCGCCAGCATCATCACGGTGTTCAGCGTTTCGGAAGATGGTAGCGTGCTGGCGGTGGAAGGCTATCAGCCTACCGAGACCCAGCCGCGCGGCTTTAACCTCGATCACAGCGGTAAATATCTCATTGCCGCCGGGCAGAAGTCGCACCATATCGCGGTGTACGATATTGTCGGTGAGCAGGGACTGCTGCAGGAGAAAGGCCGCTATGCGGTTGGGCAGGGGCCGATGTGGGTGGTGGTCAACGCCCAC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,437 total letters Query= IDP96858.102 Length=1398 Score E Sequences producing significant alignments: (Bits) Value 2_07B_B1_IDP96858.102_T7term.seq 1880 0.0 0_wt 1792 0.0 1_01B_B1_IDP96858.102_T7S.seq 1721 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 60 CCCCTCT-AGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGT 148 1 20 .......T.....-......................................................---------------------- 86 S H P Q F E K G T E N L Y F Q S N A M K Q T V Y T A S P E Query_1 149 CTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTA-CTTCCAAT-CCAATGCCATGAAACAAACCGTTTATACCGCCAGCCCGGAAAG 236 2 1151 ......NN.....C..T.....N....NG...C.......A.NNNN-....N........ 1093 0 1 ................................... 35 1 87 --------------------------...........-........-........................................... 148 S Q Q I H V W S L E A D G K L T L V Q V V D A P G Q V Q P M Query_1 237 CCAACAAATCCATGTCTGGAGTCTTGAAGCGGACGGCAAACTGACGCTGGTGCAGG-TGGTTGATGCCCCAGGGCAGGTCCAGCCGATGG 325 2 1092 ...-.N......AN..........-..............-................G................................. 1006 0 36 ........................................................-................................. 124 1 149 ........................................................-................................. 237 V V S P N K E F L Y V G V R P E F R V L A Y R I T P D N G A Query_1 326 TGGTCAGCCCCAACAAGGAATTTCTGTACGTTGGCGTTCGTCCGGA-GTTTCGCGTGCTGGCCTATCGCATCACGCCGGATAACGGGGCG 414 2 1005 ................A...................N.........A.............................N............. 916 0 125 ..............................................-........................................... 213 1 238 ..............................................-........................................... 326 L T F A G E A A L P G S P T H I S T D R H G R F V F S A S Query_1 415 TTAACCTTTGCGGGCGAAGCTGCGCTGCCGGGCA-GCCCGACCCATATCTCTACCGATCGTCATGGCCGCTTTGTCTTCAGCGCCTCCTA 503 2 915 ..............-....N..............A....................................................... 827 0 214 ..................................-....................................................... 302 1 327 ..................................-....................................................... 415 Y N Q G C V S V T P L H D G L P G E T I T V V E G L E G C H Query_1 504 CAATCAGGGCTGTGTCAGCGTGACCCCGCTGCACGACGGCCTGCCGGGCGAAACCATCACCGTGGTGGAAGGGCTGGAAGGCTGCCACTC 593 2 826 .......................................................................................... 737 0 303 .......................................................................................... 392 1 416 .......................................................................................... 505 S A N I S P D N R T L W V P A L K Q D R I C L F T L S D D G Query_1 594 GGCCAATATCTCCCCGGATAATCGCACCCTGTGGGTGCCGGCGCTGAAGCAGGATCGCATTTGCCTGTTTACCCTCAGCGACGATGGTTT 683 2 736 .......................................................................................... 647 0 393 .......................................................................................... 482 1 506 .......................................................................................... 595 F L S A Q E P A E V T T V E G A G P R H M V F H P N Q Q Y G Query_1 684 CCTTTCGGCACAGGAGCCCGCGGAAGTGACGACCGTCGAGGGCGCCGGTCCGCGTCACATGGTATTCCATCCCAACCAGCAGTATGGTTA 773 2 646 .......................................................................................... 557 0 483 .......................................................................................... 572 1 596 .......................................................................................... 685 Y C V N E L N S S I D V W E L K D P K G N I E C V Q T L D M Query_1 774 CTGCGTCAATGAGCTAAACAGCTCGATTGACGTCTGGGAGCTGAAAGATCCTAAGGGCAACATCGAATGTGTACAGACCCTGGATATGAT 863 2 556 .......................................................................................... 467 0 573 .......................................................................................... 662 1 686 .......................................................................................... 775 M P P D F S G V R W A A D I H I T P D G R H L Y A C D R T A Query_1 864 GCCGCCTGATTTTAGCGGCGTGCGCTGGGCGGCGGATATTCATATCACCCCGGATGGCCGTCATCTTTACGCCTGCGATCGTACCGCCAG 953 2 466 .......................................................................................... 377 0 663 .......................................................................................... 752 1 776 ....N..................................................................................... 865 S I I T V F S V S E D G S V L A V E G Y Q P T E T Q P R G F Query_1 954 CATCATCACGGTGTTCAGCGTTTCGGAAGATGGTAGCGTGCTGGCGGTGGAAGGCTA-TCAGCCTACCGAGACCCAGCCGCGCGGCTTTA 1042 2 376 .........................................................-................................ 288 2 291 ............ 302 0 753 .........................................................-................................ 841 0 838 ............ 827 1 866 ..........................-..............................N...-N.............N............. 953 N L D H S G K Y L I A A G Q K S H H I A V Y D I V G E Q G L Query_1 1043 ACCTCGATCACAGCGGTAAATATCTCATTGCCGCCGGGCAGAAGTCGCACCATATCGCGGTGTACGA-TATTGTCGGTGAGCAGGGACTG 1131 2 287 ...................................................................-...................... 199 0 842 ...................................................................-...................... 930 1 954 .........N.......-....N..................-................-........N.......-.........ACT.. 1039 L Q E K G R Y A V G Q G P M W V V V N A H * H W K W I T D Query_1 1132 CTGCAGGAGAAAGGCCGCT-ATGCGGTTGGGCAGGGGCCGATGTGGGTGGTGGTCAACGCCCACTAACATTGGAAGTGGATAACGGATCC 1220 2 198 ...................-...................................................................... 110 0 931 ...................-............................................ 993 1 1040 ...N..ANA.N........N.....N.G..T..-...NN...-N...N..NTNN..NNNNN...............N............T 1127 P N S S S V D K L A A A L E H H H H H H * D P A A N K A Query_1 1221 GAATTCGAGC-TCCG-TCGA--CAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacT-GAGATCCGGCTGCTAAC-AAAGCCCG 1304 2 109 ..........-....-....--.........................................G.................A........ 24 1 1128 ..........T....T....TNT.......N.. 1160 R K E Query_1 1305 AAAGGAA 1311 2 23 ...A... 17 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4683598 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 3,437 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96860.102 (IDP96860.1-292.F..pMCSG53)
MTQHDQLHRYLFENYAVRGELVTVSETLEQILANHTYPQPVKTVLAELLVATSLLTATLKFAGDITVQLQGDGPLQLAVINGNNQQQLRGVARVQGEIADDADLKTMVGNGYLVITISPEEGERYQGVVGLEGDTLAACLEDYFQRSEQLPTRLIIRTGDHEGQPMAGGMLLQVMPAQDAQTADFEHLATLTETIKAEELFTLPANDVLWRLYHEEEVTVYDPQSVEFKCTCSRERCAGALKTLPDEEIDSILADEGEIDMHCDYCGNHYIFNAMDIAEIRNNASPADPQVH
ATGACTCAACACGATCAATTACATCGTTATCTGTTTGAAAACTATGCCGTGCGCGGCGAGCTGGTGACGGTGTCGGAAACCCTGGAACAGATTCTGGCGAATCACACCTATCCCCAACCGGTCAAAACGGTGCTGGCGGAGCTGTTGGTCGCCACCAGTCTGCTGACCGCGACGCTGAAGTTTGCCGGCGACATTACCGTACAGCTGCAGGGGGATGGCCCTTTACAGCTGGCGGTGATCAACGGCAATAACCAGCAGCAGCTGCGCGGCGTCGCGCGCGTGCAGGGCGAGATTGCGGACGATGCGGATCTGAAAACGATGGTCGGCAACGGCTACCTGGTCATTACCATCTCTCCGGAAGAAGGTGAGCGCTATCAGGGCGTGGTCGGTCTGGAAGGCGATACGCTGGCCGCCTGCCTGGAAGATTATTTCCAGCGTTCAGAGCAGCTGCCGACGCGCCTTATCATCCGCACCGGCGATCATGAAGGCCAGCCGATGGCGGGCGGGATGCTGCTGCAGGTGATGCCGGCGCAGGATGCCCAGACTGCCGACTTCGAGCACCTGGCGACGCTTACCGAGACCATTAAGGCGGAAGAGCTGTTCACCCTGCCGGCAAATGATGTGCTGTGGCGCCTGTATCATGAAGAAGAAGTCACGGTCTACGACCCGCAGAGCGTGGAGTTCAAGTGCACCTGCTCCCGCGAACGCTGCGCCGGCGCGCTGAAGACGCTGCCGGATGAAGAGATTGATAGCATCCTCGCCGACGAAGGCGAAATTGACATGCACTGCGACTACTGCGGTAACCACTATATCTTCAATGCCATGGACATCGCCGAGATCCGTAATAACGCCTCTCCGGCCGATCCGCAGGTTCAT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,370 total letters Query= IDP96860.102 Length=1281 Score E Sequences producing significant alignments: (Bits) Value 1_01A_A1_IDP96860.102_T7S.seq 1844 0.0 2_07A_A1_IDP96860.102_T7term.seq 1831 0.0 0_wt 1581 0.0 R S R E I N T T H Y R G I V S G * Q F P S R N N F V Query_1 4 CGAT-CCCGCGAAATTAATAC-GACTCACTA--TAGGGGAA-TTGTG-AGCGGATAACAATT--CCCCTCTAG--AAATAATTTTG-TTT 82 1 17 .........--...........-... 39 2 1165 ....N................C.....N...AT........A..N..N.N....ATNN....TC...N..NNNGA...N.N.....N... 1076 * L * E G D I H M H H H H H H S S G V D L W S H P Q F E K G Query_1 83 AACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTA 172 1 40 ..........................................N.---------------------------------------------- 83 2 1075 .....-.........N............................---------------------------------------------- 1033 T E N L Y F Q S N A M T Q H D Q L H R Y L F E N Y A V R G E Query_1 173 CCGAGAACCTGTACTTCCAATCC-AATGCCATGACTCAACACGATCAATTACATCGTTATCTGTTTGAAAACTATGCCGTGCGCGGCGAG 261 1 84 --.....................-.................................................................. 170 2 1032 --...G...N.N...........C.................................................................. 945 0 1 ............................................................ 60 L V T V S E T L E Q I L A N H T Y P Q P V K T V L A E L L V Query_1 262 CTGGTGACGGTGTCGGAAACCCTGGAACAGATTCTGGCGAATCACACCTATCCCCAACCGGTCAAAACGGTGCTGGCGGAGCTGTTGGTC 351 1 171 .......................................................................................... 260 2 944 .......................................................................................... 855 0 61 .......................................................................................... 150 A T S L L T A T L K F A G D I T V Q L Q G D G P L Q L A V I Query_1 352 GCCACCAGTCTGCTGACCGCGACGCTGAAGTTTGCCGGCGACATTACCGTACAGCTGCAGGGGGATGGCCCTTTACAGCTGGCGGTGATC 441 1 261 .......................................................................................... 350 2 854 .......................................................................................... 765 0 151 .......................................................................................... 240 N G N N Q Q Q L R G V A R V Q G E I A D D A D L K T M V G N Query_1 442 AACGGCAATAACCAGCAGCAGCTGCGCGGCGTCGCGCGCGTGCAGGGCGAGATTGCGGACGATGCGGATCTGAAAACGATGGTCGGCAAC 531 1 351 .......................................................................................... 440 2 764 .......................................................................................... 675 0 241 .......................................................................................... 330 G Y L V I T I S P E E G E R Y Q G V V G L E G D T L A A C L Query_1 532 GGCTACCTGGTCATTACCATCTCTCCGGAAGAAGGTGAGCGCTATCAGGGCGTGGTCGGTCTGGAAGGCGATACGCTGGCCGCCTGCCTG 621 1 441 .......................................................................................... 530 2 674 .......................................................................................... 585 0 331 .......................................................................................... 420 E D Y F Q R S E Q L P T R L I I R T G D H E G Q P M A G G M Query_1 622 GAAGATTATTTCCAGCGTTCAGAGCAGCTGCCGACGCGCCTTATCATCCGCACCGGCGATCATGAAGGCCAGCCGATGGCGGGCGGGATG 711 1 531 .......................................................................................... 620 2 584 .......................................................................................... 495 0 421 .......................................................................................... 510 L L Q V M P A Q D A Q T A D F E H L A T L T E T I K A E E L Query_1 712 CTGCTGCAGGTGATGCCGGCGCAGGATGCCCAGACTGCCGACTTCGAGCACCTGGCGACGCTTACCGAGACCATTAAGGCGGAAGAGCTG 801 1 621 .......................................................................................... 710 2 494 .......................................................................................... 405 0 511 .......................................................................................... 600 F T L P A N D V L W R L Y H E E E V T V Y D P Q S V E F K C Query_1 802 TTCACCCTGCCGGCAAATGATGTGCTGTGGCGCCTGTATCATGAAGAAGAAGTCACGGTCTACGACCCGCAGAGCGTGGAGTTCAAGTGC 891 1 711 .......................................................................................... 800 2 404 .......................................................................................... 315 0 601 .......................................................................................... 690 T C S R E R C A G A L K T L P D E E I D S I L A D E G E I D Query_1 892 ACCTGCTCCCGCGAACGCTGCGCCGGCGCGCTGAAGACGCTGCCGGATGAAGAGATTGATAGCATCCTCGCCGACGAAGGCGAAATTGAC 981 1 801 .......................................................................................... 890 2 314 .......................................................................................... 225 0 691 .......................................................................................... 780 M H C D Y C G N H Y I F N A M D I A E I R N N A S P A D P Q Query_1 982 ATGCACTGCGACTACTGCGGTAACCACTATATCTTCAATGCCATGGACATCGCCGAGATCCGTAATAACGCCTCTCCGGCCGATCCGCAG 1071 1 891 .......................................................................................... 980 2 224 .......................................................................................... 135 0 781 .......................................................................................... 870 V H * H W K W I T D P N S S S V D K L A A A L E H H H H H H Query_1 1072 GTTCATTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccac 1161 1 981 ...................-........N......................N.......................-----A...ACA.N. 1064 2 134 .......................................................................................... 45 0 871 ...... 876 * D P A A N K A R K E A E L A A A T A E Q * L A * P L G A S Query_1 1162 TGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCT 1251 1 1065 ....................--G.N....-.N......AG.T...................-.................NN..NN.T... 1150 2 44 .............................-..... 11 K R V L R G F L L Query_1 1252 AAACGGGTCTTGAGGGGTTTTTTGCTGA 1279 1 1151 ..--CN.NN....A...--......... 1174 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4202330 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 3,370 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96862.102 (IDP96862.1-147.F..pMCSG53)
MAEVGIFVGTMYGNSLLVAEEAEAILSGLGHKATVYEDPQVNDWESYTGKYVLVVTSTTGQGDLPDSIVPLYEGIKDMYQPHLRYGIIALGDSTYANFCGGGLKFDQLLQEQGAKRIGEMLKIDASEDPEPESVSNPWVEQWATLLA
ATGGCGGAAGTCGGAATTTTTGTCGGCACCATGTACGGCAACTCGCTGCTGGTCGCGGAAGAAGCGGAAGCGATCCTCAGCGGACTGGGACACAAGGCTACCGTGTATGAGGATCCGCAGGTCAACGACTGGGAATCCTACACGGGTAAGTATGTGCTGGTGGTCACCTCCACCACCGGGCAGGGCGATCTGCCGGACAGCATCGTCCCGCTGTATGAGGGCATCAAAGATATGTATCAGCCTCACCTGCGGTACGGCATTATCGCGCTCGGCGACAGCACCTATGCCAACTTTTGCGGCGGCGGCCTGAAGTTCGATCAGCTGCTGCAGGAGCAGGGCGCGAAGCGCATCGGCGAGATGCTGAAGATTGACGCCAGCGAAGACCCGGAGCCGGAGAGCGTCTCCAATCCGTGGGTTGAGCAGTGGGCCACCCTGCTCGCA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,828 total letters Query= IDP96862.102 Length=846 Score E Sequences producing significant alignments: (Bits) Value 1_01C_C1_IDP96862.102_T7S.seq 1223 0.0 2_07C_C1_IDP96862.102_T7term.seq 1175 0.0 0_wt 796 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L Query_1 1 TCTCGATCCCGCGAAATT-AATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCT-AGAAATAATTTTGTTTAACTTT 88 1 21 .......N...................... 50 2 728 ..................T................................................-...................... 640 2 660 ...G...........T.T.C.C.C...........A.-.. 698 * E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E Query_1 89 AAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGA 178 1 51 ......................................------------------------------------------------.... 92 2 639 ......................................------------------------------------------------.... 598 N L Y F Q S N A M A E V G I F V G T M Y G N S L L V A E E A Query_1 179 ACCTGTACTTCCAATCCAATGCCATGGCGGAAGTCGGAATTTTTGTCGGCACCATGTACGGCAACTCGCTGCTGGTCGCGGAAGAAGCGG 268 1 93 .......................................................................................... 182 2 597 .......................................................................................... 508 0 1 ................................................................... 67 E A I L S G L G H K A T V Y E D P Q V N D W E S Y T G K Y V Query_1 269 AAGCGATCCTCAGCGGACTGGGACACAAGGCTACCGTGTATGAGGATCCGCAGGTCAACGACTGGGAATCCTACACGGGTAAGTATGTGC 358 1 183 .......................................................................................... 272 2 507 .......................................................................................... 418 0 68 .......................................................................................... 157 L V V T S T T G Q G D L P D S I V P L Y E G I K D M Y Q P H Query_1 359 TGGTGGTCACCTCCACCACCGGGCAGGGCGATCTGCCGGACAGCATCGTCCCGCTGTATGAGGGCATCAAAGATATGTATCAGCCTCACC 448 1 273 .......................................................................................... 362 2 417 .......................................................................................... 328 0 158 .......................................................................................... 247 L R Y G I I A L G D S T Y A N F C G G G L K F D Q L L Q E Q Query_1 449 TGCGGTACGGCATTATCGCGCTCGGCGACAGCACCTATGCCAACTTTTGCGGCGGCGGCCTGAAGTTCGATCAGCTGCTGCAGGAGCAGG 538 1 363 .......................................................................................... 452 2 327 .......................................................................................... 238 2 1003 ..... 999 0 248 .......................................................................................... 337 G A K R I G E M L K I D A S E D P E P E S V S N P W V E Q W Query_1 539 GCGCGAAGCGCATCGGCGAGATGCTGAAGATTGACGCCAGCGAAGACCCGGAGCCGGAGAGCGTCTCCAATCCGTGGGTTGAGCAGTGGG 628 1 453 .......................................................................................... 542 2 237 .......................................................................................... 148 2 998 ..... 994 0 338 .......................................................................................... 427 A T L L A * H W K W I T D P N S S S V D K L A A A L E H H H Query_1 629 CCACCCTGCTCGCATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccacc 718 1 543 .......................................................................................... 632 2 147 .......................................................................................... 58 0 428 .............. 441 H H H * D P A A N K A R K E A E L A A A T A E Q * L A * P Query_1 719 accaccacTGAGATCCGGCTGCTAACAAAG-CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCAT-AACCCCT 806 1 633 ..............................-...................................................A....... 721 2 57 ..............................C...N....... 16 L G A S K R V L R G F L L K Query_1 807 TGGGGCCTCTAAACGGGTC-TTGAGGGGTTTTTTGCTGAAA 846 1 722 ...................T..................... 762 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2312673 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 2,828 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96864.103 (IDP96864.185-1411.P(1411)..pMCSG68)
SIAEKLGGGILSVLTFASNRTRRDDTWVDEGEYEDDEEEYDDEEAARPQESRRARILRSALARRKRLAEKFTNPMGRKTDAALFSGKRMDDGEEVVQYSASGAPVAADDVLFSGASAARPAEDDVLFSGASAVRPGDFDPYDPLLNGHSIAEPVSAAAAATAAPQAWAESPVGHHGAAPAYQPEASYPPQQAYQPEPAPFQQAYQPEPAPFQQAAYQPPAGQTAPQAYQPEPAPYQQPVYDPRAGQPAPQAYQPEPAPYQQPAYDPYAGQPAPQAYQPEPAPYQQPAYDPHAGQPAPQAYQPEPAPYQQPAYDPYAGQPAPQAYQPEPAPYQQPTYDPYAGQPAPQTYQQPAYDPNAGQPAPQPYQPEPAAYQPQSAPVPPPEPEPEVVQEEVKRPPLYYFEEVEEKRARERELLASWYQPIPEPESPIATKPLTPPTTASKPPVETTVVSAVAAGVHQATAASGGAAAATSSTAASAAATPLFSPASSGPRVQVKEGIGPKLPRPNRVRVPTRRELASYGIKLPSQREAEQRARQAERDPHYDDELLSDEEADAMEQDELARQFAATQQQRYGHRWEDDNATDDDEADAAAEAELARQFAATQQQRYATEQPPGANPFSPADYEFSPMKTLVNDGPSEPLFTPTPEVQPQQPAQRYQQPAAAPQQGYQPAQHQPIHHQPVPPQPQSYPTASQPVQPQQPVAPQGHQPAAPAPQESLIHPLLMRNGDSRPLQKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGDPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWSVNEMERRYKLMSALGVRNLAGYNEKIAEAARMGRPIPDPYWKPGDSMDAVHPVLEKLPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILDQGGAESLLGMGDMLYSGPNSTTPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSDSESEGGGGGFDGGEELDPLFDQAVNFVTEKRKASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFE
AGCATTGCGGAAAAGCTGGGCGGCGGCATCCTGTCCGTTCTCACCTTTGCCAGCAACCGTACCCGTCGGGATGATACCTGGGTCGATGAAGGCGAATATGAAGACGACGAGGAAGAGTACGACGACGAAGAGGCGGCCAGGCCGCAGGAATCGCGTCGCGCCCGTATCTTACGCAGCGCGCTGGCGCGGCGTAAGCGTCTGGCCGAGAAGTTTACCAACCCTATGGGGCGTAAAACCGACGCTGCGCTTTTCTCCGGCAAACGGATGGATGACGGCGAAGAGGTGGTGCAATACAGCGCCAGCGGGGCGCCTGTTGCCGCCGACGATGTACTGTTTTCCGGCGCCAGCGCCGCGCGTCCCGCAGAGGATGATGTGCTGTTCTCCGGCGCCAGCGCCGTGCGCCCGGGCGATTTCGACCCTTACGATCCGTTGTTGAATGGCCACAGTATCGCTGAGCCGGTAAGCGCAGCGGCGGCGGCTACAGCCGCGCCGCAGGCGTGGGCAGAATCACCGGTGGGCCATCACGGCGCTGCGCCAGCTTATCAGCCGGAAGCCAGCTATCCGCCGCAGCAGGCCTATCAGCCTGAACCCGCTCCGTTCCAGCAGGCCTATCAGCCTGAACCCGCTCCGTTCCAGCAGGCTGCTTATCAGCCGCCAGCGGGGCAAACCGCACCGCAGGCGTATCAGCCTGAGCCAGCGCCGTATCAACAGCCGGTTTACGATCCGCGTGCCGGTCAACCTGCGCCGCAGGCCTATCAGCCTGAGCCAGCGCCGTATCAGCAGCCGGCTTACGATCCGTATGCCGGTCAACCTGCGCCGCAGGCCTATCAGCCTGAACCTGCGCCGTATCAGCAGCCGGCTTACGATCCGCATGCCGGTCAACCTGCACCGCAGGCCTATCAGCCTGAGCCAGCGCCGTATCAGCAGCCGGCTTACGATCCCTATGCCGGTCAACCTGCGCCGCAGGCCTATCAGCCGGAGCCAGCGCCGTATCAGCAGCCAACTTACGATCCCTATGCCGGTCAGCCTGCGCCTCAGACCTATCAGCAGCCGGCTTACGATCCGAATGCCGGTCAGCCCGCGCCGCAGCCGTATCAGCCGGAGCCAGCGGCGTATCAGCCGCAAAGCGCGCCAGTTCCCCCACCGGAGCCAGAGCCCGAGGTCGTGCAGGAGGAAGTGAAACGTCCGCCGCTCTATTATTTCGAGGAAGTGGAAGAGAAGCGGGCGCGCGAACGCGAGCTGTTGGCCTCCTGGTATCAGCCAATTCCTGAGCCGGAAAGTCCGATTGCCACTAAACCGCTGACGCCGCCGACCACTGCGTCCAAACCGCCAGTGGAGACAACCGTAGTCTCTGCGGTAGCGGCTGGGGTGCATCAGGCTACCGCCGCCAGCGGCGGCGCGGCGGCAGCAACCTCGTCCACTGCCGCATCCGCTGCGGCTACGCCATTGTTCAGCCCGGCGTCCAGCGGCCCAAGGGTTCAGGTGAAAGAGGGCATCGGTCCAAAACTACCGCGGCCCAATCGCGTGCGTGTTCCTACGCGTCGGGAACTGGCCTCCTACGGCATCAAGCTACCGTCGCAGCGGGAGGCGGAACAGCGCGCGCGGCAGGCGGAGCGCGATCCGCATTATGATGATGAGCTGCTCTCGGATGAGGAAGCGGATGCTATGGAGCAGGATGAACTGGCTCGCCAGTTCGCCGCCACCCAGCAGCAGCGCTACGGTCATCGCTGGGAAGACGATAACGCGACTGATGACGATGAGGCCGACGCCGCGGCGGAAGCGGAGCTGGCGCGTCAGTTTGCCGCTACCCAGCAGCAGCGGTACGCTACCGAGCAGCCGCCGGGCGCCAACCCGTTCTCGCCGGCAGATTATGAATTCTCGCCGATGAAAACGTTGGTCAATGACGGCCCGAGCGAACCGCTGTTTACGCCGACGCCGGAAGTCCAGCCGCAGCAGCCGGCCCAGCGCTATCAACAACCGGCGGCCGCTCCGCAGCAGGGTTATCAACCTGCGCAGCATCAGCCGATACACCATCAGCCTGTGCCGCCACAGCCGCAGTCCTATCCGACTGCGTCGCAGCCCGTACAGCCGCAACAACCGGTTGCCCCGCAGGGGCATCAGCCTGCCGCCCCTGCGCCGCAGGAGAGCCTGATCCACCCGCTGCTGATGCGCAATGGCGATAGTCGACCGCTGCAAAAGCCGACCACGCCACTGCCGTCGCTGGATCTGCTTACCCCGCCGCCGAGTGAAGTCGAGCCGGTGGATACCTTTGCTCTCGAGCAGATGGCACGCCTGGTGGAAGCGCGACTCGCTGATTTCCGCATTAAAGCGGATGTGGTGAACTACTCACCGGGGCCGGTGATCACCCGCTTCGAACTGAATCTGGCGCCTGGCGTTAAGGCCGCACGGATCTCTAACCTGTCACGGGACCTGGCGCGATCGCTGTCAACGGTCGCCGTGCGCGTGGTGGAGGTGATCCCGGGCAAACCGTATGTCGGGCTTGAGCTGCCGAATAAAAAACGCCAGACCGTCTACCTGCGTGAAGTGCTCGACAACGCCAAGTTCCGTGATAACCCATCTCCGCTCACCGTGGTGTTGGGTAAAGACATCGCTGGCGATCCGGTAGTAGCCGATCTGGCGAAAATGCCGCATCTGCTGGTGGCCGGTACCACCGGTTCCGGTAAGTCTGTTGGCGTCAACGCCATGATCCTCAGCATGCTCTACAAGGCGCAGCCGGAAGATGTGCGTTTCATTATGATCGACCCGAAAATGCTCGAGCTGTCGGTCTACGAAGGAATTCCGCACCTGCTGACGGAAGTGGTCACCGACATGAAAGACGCCGCCAATGCGCTGCGCTGGAGCGTCAATGAGATGGAGCGCCGCTACAAGCTGATGTCGGCGCTGGGCGTGCGTAACCTCGCGGGCTACAACGAGAAGATCGCCGAAGCCGCGCGCATGGGACGTCCGATCCCGGATCCGTACTGGAAGCCTGGCGACAGCATGGACGCCGTACATCCGGTGCTGGAAAAACTGCCGTACATCGTGGTGCTGGTGGATGAATTCGCCGATCTGATGATGACCGTCGGCAAAAAGGTGGAAGAGCTGATCGCTCGCCTGGCGCAGAAAGCGCGCGCGGCGGGGATCCACCTGGTGCTGGCGACACAGCGTCCGTCGGTAGATGTTATTACCGGCCTGATTAAGGCCAACATCCCGACGCGCATCGCCTTTACCGTGTCGAGTAAAATTGACTCACGTACCATTCTCGATCAGGGCGGCGCGGAATCGCTGCTGGGTATGGGGGATATGCTTTACTCCGGGCCGAACTCTACCACGCCGGTGCGTGTCCACGGGGCGTTTGTGCGCGACCAGGAAGTCCACGCCGTGGTTCAGGACTGGAAAGCCCGCGGTCGCCCGCAATATGTGGATGGCATTACCTCCGACAGCGAAAGCGAAGGCGGCGGTGGCGGCTTCGACGGCGGGGAAGAGTTGGATCCGTTGTTCGATCAGGCAGTCAACTTTGTGACCGAGAAGCGCAAAGCGTCGATTTCCGGGGTTCAGCGTCAGTTCCGCATCGGCTATAACCGTGCCGCGCGTATTATCGAACAGATGGAAGCGCAGGGTATCGTCAGCGAGCAGGGCCATAACGGTAACCGCGAAGTGCTGGCGCCGCCGCCCTTTGAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,691 total letters Query= IDP96864.103 Length=4086 Score E Sequences producing significant alignments: (Bits) Value 0_wt 6639 0.0 S I A E K L G G G I L S V L T F A S N R T R R D D T W V D E Query_1 202 AGCATTGCGGAAAAGCTGGGCGGCGGCATCCTGTCCGTTCTCACCTTTGCCAGCAACCGTACCCGTCGGGATGATACCTGGGTCGATGAA 291 0 1 .......................................................................................... 90 G E Y E D D E E E Y D D E E A A R P Q E S R R A R I L R S A Query_1 292 GGCGAATATGAAGACGACGAGGAAGAGTACGACGACGAAGAGGCGGCCAGGCCGCAGGAATCGCGTCGCGCCCGTATCTTACGCAGCGCG 381 0 91 .......................................................................................... 180 L A R R K R L A E K F T N P M G R K T D A A L F S G K R M D Query_1 382 CTGGCGCGGCGTAAGCGTCTGGCCGAGAAGTTTACCAACCCTATGGGGCGTAAAACCGACGCTGCGCTTTTCTCCGGCAAACGGATGGAT 471 0 181 .......................................................................................... 270 D G E E V V Q Y S A S G A P V A A D D V L F S G A S A A R P Query_1 472 GACGGCGAAGAGGTGGTGCAATACAGCGCCAGCGGGGCGCCTGTTGCCGCCGACGATGTACTGTTTTCCGGCGCCAGCGCCGCGCGTCCC 561 0 271 .......................................................................................... 360 0 653 ............ 664 A E D D V L F S G A S A V R P G D F D P Y D P L L N G H S I Query_1 562 GCAGAGGATGATGTGCTGTTCTCCGGCGCCAGCGCCGTGCGCCCGGGCGATTTCGACCCTTACGATCCGTTGTTGAATGGCCACAGTATC 651 0 361 .......................................................................................... 450 0 788 ............------...---C.G..CAA 810 0 976 ....A........... 991 0 693 ........... 703 0 765 ........... 775 0 909 ........... 919 0 3490 ........... 3500 A E P V S A A A A A T A A P Q A W A E S P V G H H G A A P Query_1 652 GCTGAGCCGGTAAGCGCAGCGGCGGCGGCTACAGCCGCGCCG---CAGGCGTGGGCAGAATCACCGGTGGGCCATCACGGCGCTGCGCCA 738 0 451 ..........................................---............................................. 537 0 811 C...C...-.C.G..CT.T.A..-----..GA.C.T......TAT...CA.CC...TT.CGAT...CAT.C.GG...ACCT..AC...AG 894 0 783 ...G 786 0 855 ...G 858 0 1079 ........---...C... 1093 0 742 ......---..... 752 0 958 ......---..... 968 0 1389 ........... 1399 A Y Q P E A S Y P P Q Q A Y Q P E P A P F Q Q Query_1 739 GCTTATCAGCCGGAAGCCAGC----TATCCG---CCGCAGCAGGCCTATCAGCCTGAACCCGCTC--CGTTC-CAGCAG----------- 807 0 538 .....................----......---...............................--.....-......----------- 606 0 747 ....----------- 750 0 819 ....----------- 822 0 891 ....----------- 894 0 895 ..C........T.-.......------G...TAT.A.....C...T..CG.T..CT.TG...G..AA.C.G.G.C....----------- 966 0 959 ...C...............G..G..A..G.--...AT-......CCAACTTACGA 1010 0 787 .....CG.T...T.T...G.T----C.A..T---G...C.....................T..G.--... 847 0 859 .....CG.T...C.T...G.T----C.A..T---G.A.C..................G..A..G.--... 919 0 743 ...C..................G..A..G.--... 775 0 967 ..C..........-....... 986 0 1093 ..........-.......GGCG....A.---..... 1124 A Y Q P E P A P F Q Q A A Y Q P P A G Q T A P Q A Y Q Query_1 808 -------GCCTATCAGCCTGAACCCGCTCCGTTCCAGCAGGCTGCTTATCAGCCGCCAGCGGGGCAAACCGCACCGCAGGCGTATCAGCC 890 0 607 -------................................................................................... 689 0 751 -------..............G..A..G....AT......C.G.....CG.T...TAT..C..T...C.T..G........C........ 833 0 823 -------.................T..G....AT......C.G.....CG.T....AT..C..T...C.T...........C........ 905 0 895 -------..............G..A..G....AT......C.G.....CG.T..CTAT..C..T...C.T..G........C........ 977 0 967 -------...........G..G..A..G....AT......C.AA....---------------------..AT..CT.T..CGG...... 1028 0 1011 TCCCTAT...GG........CG..TCAGA.C.AT......C.G.....CG.T...AAT..C..T..GC....G......C.......... 1100 0 1945 ...C..C.G..... 1958 0 297 ............ 308 0 1110 ............ 1121 0 1384 ........... 1394 P E P A P Y Q Q P V Y D P R A G Q P A P Q A Y Q P E P A P Query_1 891 TGAGCCAGCGCCGTATCAACAGCCGGTTTACGATCCGCGTGCCGG----TCAACCTGCGCCGCAGGCCTATCAGCCTGAGCCAGCGCCGT 976 0 690 .............................................----......................................... 775 0 603 ..................A..C..T.... 631 0 834 ...A..T...........G.......C...........A......----.........A............................... 919 0 906 ..................G.......C.........CTA......----...........................G............. 991 0 978 G.................G.....AAC.........CTA......----...G........T...A..---------------------. 1042 0 1029 ..C...TCA.A.C.....G.......C..........AA......----...G..C.........C.G........G.........G... 1114 0 1101 G.........G....... 1118 0 566 ...A..................A..C..T.... 598 0 1959 G.C-.......--........A....CGGC..C.....A-..A..GTTA...........A... 2018 0 1962 .......--. 1969 0 2131 ............. 2143 0 487 ........... 497 Y Q Q P A Y D P Y A G Q P A P Q A Y Q P E P A P Y Q Q P A Query_1 977 ATCAGCAGCCGGCTTACGATCCGTATGCCGG----TCAACCTGCGCCGCAGGCCTATCAGCCTGAACCTGCGCCGTATCAGCAGCCGGCT 1062 0 776 ...............................----....................................................... 861 0 632 TC......G.T.....TC.G...CCA..G..----G...A.C..A........G...........G..A...........A.......T. 717 0 920 ......................C........----...........................G..G..A.................AA.. 1005 0 603 .....................C..T....TC......G.T... 645 0 992 ..........AA..........C........----...G........T...A..---------------------............... 1056 0 1043 .......................A.......----...G..C.........C.G........G..G..A...G.........C.. 1123 0 1115 ......C.. 1123 0 534 ...A.....TC.G...G.A...A.----CT.T..GC...A.....................C..T.... 598 0 534 ...A... 540 0 1970 ....A..A.....GGC..C....C.-..A..GTTA...........A... 2018 0 2131 ............. 2143 0 487 ........... 497 Y D P H A G Q P A P Q A Y Q P E P A P Y Q Q P A Y D P Y A Query_1 1063 TACGATCCGCATGCCGGTCAACCTGCACCGCAGGCCTATCAGCCTGAGCCAGCGCCGTATCAGCAGCCGGCTTACGATCCCTATGCCGG- 1151 0 862 .........................................................................................- 950 0 718 ..........G...............G.....................................................G........- 806 0 1006 ........CT..........G.....G..T...A..---------------------.......................GA.......- 1073 0 646 ..TC.G....CA..G..G...A.C...........G..........................A.......T.........GCG......- 734 0 1057 .........A..........G..C..G......C.G........G.........G.........C.. 1123 0 603 ..................A..C..T....TC......G.T.....TC.G..GCCA..G..- 662 0 541 ..TC.G...G.A...A.CT.T..GC.G.A..................A..C..T.... 598 0 1962 .......--.....A..A.....GGC..C...GC.-..A..G 2000 G Q P A P Q A Y Q P E P A P Y Q Q P T Y D P Y A G Q P A P Query_1 1152 ---TCAACCTGCGCCGCAGGCCTATCAGCCGGAGCCAGCGCCGTATCAGCAGCCAACTTACGATCCCTATGCCGGTCAGCCTGCGCCTCA 1238 0 951 ---....................................................................................... 1037 0 807 ---...........................T..A..T.................GG..........GC..........A.....A..G.. 893 0 1074 ---...G..C.........C.G..................G.........C..A.. 1126 0 735 ---...........................T.......................GG..........G...........A........G.. 821 0 663 ---G...A.C..A........G........T.................A.....GGT.........GCG.........A........G.. 749 0 566 ...A...............T..A..C..T....TC......------------------...TA........AA..CGC 626 0 2001 TTA...........A... 2018 0 383 ....C........... 398 0 2131 ............. 2143 0 487 ........... 497 Q T Y Q Q P A Y D P N A G Q P A P Q P Y Q P E Query_1 1239 GACC---------------------TATCAGCAGCCGGCTTACGATCCGAATGCCGGTCAGCCCGCGCCGCAGCCGTATCAGCCGG-AGC 1306 0 1038 ....---------------------.............................................................-... 1105 0 894 .G..TATCAGCCTGAGCCAGCGCCG.......................CT..........A..T.........G.C..........-... 982 0 822 .G..TATCAGCCTGAACCTGCGCCG........................C..........A..T..A......G.C........T.-... 910 0 750 .G..TATCAGCCTGAGCCAGCGCCG........................T..........A..T.........G.C........T.-.A. 838 0 627 TC.G---------------------.TC......G.T.....TC.G...CCA..G..G..AA....A......G..........T.-... 694 0 485 ...........G... 499 0 541 ..........A... 554 P A A Y Q P Q S A P V P P P E P E P E V V Q E E V K R P P L Query_1 1307 CAGCGGCGTATCAGCCGCAAAGCGCGCCAGTTCCCCCACCGGAGCCAGAGCCCGAGGTCGTGCAGGAGGAAGTGAAACGTCCGCCGCTCT 1396 0 1106 .......................................................................................... 1195 0 983 .....C.........A..C.. 1003 0 911 .....C.........A.. 928 0 839 .T...C.........A.. 856 0 695 .....C....... 707 0 678 ............ 689 0 555 ....----....C...... 569 Y Y F E E V E E K R A R E R E L L A S W Y Q P I P E P E S P Query_1 1397 ATTATTTCGAGGAAGTGGAAGAGAAGCGGGCGCGCGAACGCGAGCTGTTGGCCTCCTGGTATCAGCCAATTCCTGAGCCGGAAAGTCCGA 1486 0 1196 .......................................................................................... 1285 I A T K P L T P P T T A S K P P V E T T V V S A V A A G V H Query_1 1487 TTGCCACTAAACCGCTGACGCCGCCGACCACTGCGTCCAAACCGCCAGTGGAGACAACCGTAGTCTCTGCGGTAGCGGCTGGGGTGCATC 1576 0 1286 .......................................................................................... 1375 Q A T A A S G G A A A A T S S T A A S A A A T P L F S P A S Query_1 1577 AGGCTACCGCCGCCAGCGGCGGCGCGGCGGCAGCAACCTCGTCCACTGCCGCATCCGCTGCGGCTACGCCATTGTTCAGCCCGGCGTCCA 1666 0 1376 .......................................................................................... 1465 0 651 ........... 661 S G P R V Q V K E G I G P K L P R P N R V R V P T R R E L A Query_1 1667 GCGGCCCAAGGGTTCAGGTGAAAGAGGGCATCGGTCCAAAACTACCGCGGCCCAATCGCGTGCGTGTTCCTACGCGTCGGGAACTGGCCT 1756 0 1466 .......................................................................................... 1555 S Y G I K L P S Q R E A E Q R A R Q A E R D P H Y D D E L L Query_1 1757 CCTACGGCATCAAGCTACCGTCGCAGCGGGAGGCGGAACAGCGCGCGCGGCAGGCGGAGCGCGATCCGCATTATGATGATGAGCTGCTCT 1846 0 1556 .......................................................................................... 1645 0 3126 ............G.. 3140 S D E E A D A M E Q D E L A R Q F A A T Q Q Q R Y G H R W E Query_1 1847 CGGATGAGGAAGCGGATGCTATGGAGCAGGATGAACTGGCTCGCCAGTTCGCCGCCACCCAGCAGCAGCGCTACGGTCATCGCTGGGAAG 1936 0 1646 .......................................................................................... 1735 0 1783 ..G.....G..T.....T.....T..............G.... 1825 D D N A T D D D E A D A A A E A E L A R Q F A A T Q Q Q R Y Query_1 1937 ACGATAACGCGACTGATGACGATGAGGCCGACGCCGCGGCGGAAGCGGAGCTGGCGCGTCAGTTTGCCGCTACCCAGCAGCAGCGGTACG 2026 0 1736 .......................................................................................... 1825 0 1678 ..A.....T..C.....C.....C..............C.... 1720 0 3295 ........... 3285 A T E Q P P G A N P F S P A D Y E F S P M K T L V N D G P S Query_1 2027 CTACCGAGCAGCCGCCGGGCGCCAACCCGTTCTCGCCGGCAGATTATGAATTCTCGCCGATGAAAACGTTGGTCAATGACGGCCCGAGCG 2116 0 1826 .......................................................................................... 1915 E P L F T P T P E V Q P Q Q P A Q R Y Q Q P A A A P Q Q G Query_1 2117 AACCGCTGTTTACGCCGACGCCGGAAGTCCAGCCGCAGCAGCCGGC-CCAGCGC--TATCAACAACCGGCGGCCGCTCCGCA-GCAGGGT 2202 0 1916 ..............................................-.......--..........................-....... 2001 0 676 ...G..T.T.....T.AG.......CG........G....TTTA..A.....GT..C..-- 734 0 766 .......CG.....G..G.....TTA..A....T.T..C..-- 806 0 910 .......CG.....G..G.....TTA..A...CT.T..C..-- 950 0 778 ........... 788 0 850 ........... 860 0 922 ........... 932 0 1045 ........... 1055 Y Q P A Q H Q P I H H Q P V P P Q P Q S Y P T A S Q P V Q P Query_1 2203 TATCAACCTGCGCAGCATCAGCCGATACACCATCAGCCTGTGCCGCCACAGCCGCAGTCCTATCCGACTGCGTCGCAGCCCGTACAGCCG 2292 0 2002 .......................................................................................... 2091 0 735 --...........C... 749 0 807 --...........C... 821 0 951 --...........C... 965 Q Q P V A P Q G H Q P A A P A P Q E S L I H P L L M R N G D Query_1 2293 CAACAACCGGTTGCCCCGCAGGGGCATCAGCCTGCCGCCCCTGCGCCGCAGGAGAGCCTGATCCACCCGCTGCTGATGCGCAATGGCGAT 2382 0 2092 .......................................................................................... 2181 0 739 ............. 751 0 811 ............. 823 0 955 ............. 967 S R P L Q K P T T P L P S L D L L T P P P S E V E P V D T F Query_1 2383 AGTCGACCGCTGCAAAAGCCGACCACGCCACTGCCGTCGCTGGATCTGCTTACCCCGCCGCCGAGTGAAGTCGAGCCGGTGGATACCTTT 2472 0 2182 .......................................................................................... 2271 A L E Q M A R L V E A R L A D F R I K A D V V N Y S P G P V Query_1 2473 GCTCTCGAGCAGATGGCACGCCTGGTGGAAGCGCGACTCGCTGATTTCCGCATTAAAGCGGATGTGGTGAACTACTCACCGGGGCCGGTG 2562 0 2272 .......................................................................................... 2361 I T R F E L N L A P G V K A A R I S N L S R D L A R S L S T Query_1 2563 ATCACCCGCTTCGAACTGAATCTGGCGCCTGGCGTTAAGGCCGCACGGATCTCTAACCTGTCACGGGACCTGGCGCGATCGCTGTCAACG 2652 0 2362 .......................................................................................... 2451 V A V R V V E V I P G K P Y V G L E L P N K K R Q T V Y L R Query_1 2653 GTCGCCGTGCGCGTGGTGGAGGTGATCCCGGGCAAACCGTATGTCGGGCTTGAGCTGCCGAATAAAAAACGCCAGACCGTCTACCTGCGT 2742 0 2452 .......................................................................................... 2541 E V L D N A K F R D N P S P L T V V L G K D I A G D P V V A Query_1 2743 GAAGTGCTCGACAACGCCAAGTTCCGTGATAACCCATCTCCGCTCACCGTGGTGTTGGGTAAAGACATCGCTGGCGATCCGGTAGTAGCC 2832 0 2542 .......................................................................................... 2631 D L A K M P H L L V A G T T G S G K S V G V N A M I L S M L Query_1 2833 GATCTGGCGAAAATGCCGCATCTGCTGGTGGCCGGTACCACCGGTTCCGGTAAGTCTGTTGGCGTCAACGCCATGATCCTCAGCATGCTC 2922 0 2632 .......................................................................................... 2721 Y K A Q P E D V R F I M I D P K M L E L S V Y E G I P H L L Query_1 2923 TACAAGGCGCAGCCGGAAGATGTGCGTTTCATTATGATCGACCCGAAAATGCTCGAGCTGTCGGTCTACGAAGGAATTCCGCACCTGCTG 3012 0 2722 .......................................................................................... 2811 T E V V T D M K D A A N A L R W S V N E M E R R Y K L M S A Query_1 3013 ACGGAAGTGGTCACCGACATGAAAGACGCCGCCAATGCGCTGCGCTGGAGCGTCAATGAGATGGAGCGCCGCTACAAGCTGATGTCGGCG 3102 0 2812 .......................................................................................... 2901 L G V R N L A G Y N E K I A E A A R M G R P I P D P Y W K P Query_1 3103 CTGGGCGTGCGTAACCTCGCGGGCTACAACGAGAAGATCGCCGAAGCCGCGCGCATGGGACGTCCGATCCCGGATCCGTACTGGAAGCCT 3192 0 2902 .......................................................................................... 2991 G D S M D A V H P V L E K L P Y I V V L V D E F A D L M M T Query_1 3193 GGCGACAGCATGGACGCCGTACATCCGGTGCTGGAAAAACTGCCGTACATCGTGGTGCTGGTGGATGAATTCGCCGATCTGATGATGACC 3282 0 2992 .......................................................................................... 3081 V G K K V E E L I A R L A Q K A R A A G I H L V L A T Q R P Query_1 3283 GTCGGCAAAAAGGTGGAAGAGCTGATCGCTCGCCTGGCGCAGAAAGCGCGCGCGGCGGGGATCCACCTGGTGCTGGCGACACAGCGTCCG 3372 0 3082 .......................................................................................... 3171 0 1595 ............A.. 1609 S V D V I T G L I K A N I P T R I A F T V S S K I D S R T I Query_1 3373 TCGGTAGATGTTATTACCGGCCTGATTAAGGCCAACATCCCGACGCGCATCGCCTTTACCGTGTCGAGTAAAATTGACTCACGTACCATT 3462 0 3172 .......................................................................................... 3261 L D Q G G A E S L L G M G D M L Y S G P N S T T P V R V H G Query_1 3463 CTCGATCAGGGCGGCGCGGAATCGCTGCTGGGTATGGGGGATATGCTTTACTCCGGGCCGAACTCTACCACGCCGGTGCGTGTCCACGGG 3552 0 3262 .......................................................................................... 3351 0 1816 ........... 1806 A F V R D Q E V H A V V Q D W K A R G R P Q Y V D G I T S D Query_1 3553 GCGTTTGTGCGCGACCAGGAAGTCCACGCCGTGGTTCAGGACTGGAAAGCCCGCGGTCGCCCGCAATATGTGGATGGCATTACCTCCGAC 3642 0 3352 .......................................................................................... 3441 S E S E G G G G G F D G G E E L D P L F D Q A V N F V T E K Query_1 3643 AGCGAAAGCGAAGGCGGCGGTGGCGGCTTCGACGGCGGGGAAGAGTTGGATCCGTTGTTCGATCAGGCAGTCAACTTTGTGACCGAGAAG 3732 0 3442 .......................................................................................... 3531 R K A S I S G V Q R Q F R I G Y N R A A R I I E Q M E A Q G Query_1 3733 CGCAAAGCGTCGATTTCCGGGGTTCAGCGTCAGTTCCGCATCGGCTATAACCGTGCCGCGCGTATTATCGAACAGATGGAAGCGCAGGGT 3822 0 3532 .......................................................................................... 3621 I V S E Q G H N G N R E V L A P P P F E * H W K W I T D P N Query_1 3823 ATCGTCAGCGAGCAGGGCCATAACGGTAACCGCGAAGTGCTGGCGCCGCCGCCCTTTGAATAACATTGGAAGTGGATAACGGATCCGAAT 3912 0 3622 ............................................................ 3681 S S S V D K L A A A L E H H H H H H * D P A A N K A R K E A Query_1 3913 TCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCT 4002 E L A A Query_1 4003 GAGTTGGCTGCTG 4015 0 1005 ............ 994 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 14811160 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 3,691 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96865.104 (IDP96865.1-623.F..pMCSG53)
MNINEKTPVGEVRPSQLLWTYGPGALIDLPNLSVVTLGIDRWEKNCCQPIQETRLLAAVRKVLGDQVESLRMPPFQKSEVTDMWSAEANIGVPVRPFPRWLRCVKCGLLSPYDAGLFEIKENRFRPERTRFVHKDCRGSKGDQPARDADAVPARFLLACRDGHLDDFPWHYFVHGGNNPCKGTLRFFESGASLQTENLWVKCDSCELSRSMAHAFGKAGKENLPACRGRHPHLDYFDAECDEEPRAVLLGATNSWFPVTLSALAIPLSNVTPLHQIIIDYWDDFSDVEDLSELKGILKILNKRGEAQGIEKHAAEEIWSAIETHRSQDGEDFVGEADIKDPEWNVLIEPTPPTDYPHFMSKKTRTPTRFAPYIERVLLLERLREVNALLGFTRVEAPEESEDLNERPQMASLSRSKPDWVPANQVHGEGIFLQFNEQALVAWESLDAVKQSDKMLRGGHTGWRNARHLDPEEGYPGIRYAMLHTLSHLLIRELALECGYNAASIRERIYANTTNGTPQAGILIYTAAADSDGTLGGLVDLGKPENLGRLLEQALNRSRICSSDPLCSEHDPEKDRSLHGAACHACSLVAETSCEKGNRYLDRALLVPTLEHTEAAFFKDLLNG
ATGAACATTAATGAGAAAACACCCGTTGGTGAAGTTCGCCCGAGCCAATTGCTTTGGACATATGGTCCTGGAGCCCTAATTGATTTGCCCAACTTGTCAGTTGTAACACTTGGGATCGACAGGTGGGAAAAGAACTGTTGTCAGCCAATTCAGGAAACCCGGTTACTAGCTGCCGTTAGAAAGGTATTAGGTGATCAGGTTGAAAGCTTGCGAATGCCACCATTTCAAAAAAGCGAAGTCACAGATATGTGGTCTGCTGAAGCAAATATAGGGGTACCTGTACGTCCTTTCCCTAGATGGTTGCGATGCGTTAAATGTGGGTTACTTTCACCTTACGATGCCGGGCTTTTCGAAATAAAAGAAAATCGATTTAGGCCTGAACGAACTCGCTTTGTTCATAAAGACTGCCGGGGTTCTAAGGGCGATCAACCTGCAAGGGATGCTGATGCAGTTCCAGCCCGTTTTCTCTTGGCTTGCCGTGATGGTCATCTTGATGATTTTCCGTGGCACTATTTTGTTCATGGTGGTAACAATCCATGCAAAGGAACGCTGCGATTTTTTGAAAGTGGCGCTTCATTGCAGACAGAAAATCTTTGGGTAAAATGCGATTCCTGTGAATTATCAAGAAGTATGGCGCATGCATTTGGTAAGGCCGGTAAAGAAAATCTTCCCGCATGTAGAGGACGTCATCCTCATCTGGATTACTTTGATGCTGAATGTGACGAAGAACCTCGTGCAGTACTGCTTGGGGCTACGAATAGCTGGTTTCCTGTAACGCTATCAGCACTAGCGATCCCATTATCAAATGTTACTCCATTACATCAGATAATCATTGATTACTGGGATGATTTTTCTGATGTAGAAGATCTTTCTGAACTTAAAGGGATACTAAAAATCCTCAACAAACGTGGTGAAGCTCAAGGTATTGAAAAGCATGCTGCAGAGGAAATATGGTCTGCAATTGAAACTCATCGCTCACAAGATGGCGAGGATTTTGTCGGTGAGGCTGATATTAAAGATCCTGAATGGAATGTACTAATAGAACCTACTCCCCCCACTGATTATCCTCATTTTATGAGTAAAAAAACCCGTACTCCTACGCGGTTTGCGCCATATATTGAGCGAGTTTTACTCTTAGAACGATTACGTGAGGTTAATGCTTTACTTGGTTTTACCCGGGTCGAGGCTCCTGAAGAATCTGAAGATCTCAACGAAAGACCGCAAATGGCCAGCCTGTCACGGAGCAAACCTGATTGGGTACCCGCCAATCAGGTCCATGGTGAAGGGATATTCCTGCAGTTCAATGAACAAGCGCTTGTGGCATGGGAATCTCTGGACGCTGTAAAACAATCGGACAAAATGCTTCGCGGTGGGCATACAGGTTGGAGAAATGCTCGTCACTTGGATCCAGAAGAAGGTTATCCCGGCATTCGCTATGCTATGTTGCATACACTTTCCCATTTACTGATTCGAGAATTGGCTCTGGAATGTGGATATAATGCCGCGAGTATTCGTGAGCGTATCTATGCAAATACAACCAATGGTACTCCTCAGGCGGGTATCCTTATTTATACCGCTGCCGCTGATTCAGACGGTACTTTAGGGGGACTGGTTGATCTTGGCAAACCAGAAAATCTTGGACGTTTACTTGAACAAGCATTAAATCGCTCAAGGATCTGCTCATCAGACCCATTATGTTCAGAGCATGATCCAGAGAAGGATCGTTCGCTGCATGGAGCTGCTTGCCATGCCTGTAGCTTAGTTGCAGAAACCTCTTGCGAGAAAGGGAATCGCTATTTAGATAGAGCCCTGCTTGTACCAACACTGGAGCATACTGAAGCAGCTTTCTTCAAGGATTTATTAAATGGA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,345 total letters Query= IDP96865.104 Length=2274 Score E Sequences producing significant alignments: (Bits) Value 0_wt 3371 0.0 2_10G_G7_IDP96865.104_T7term.seq 1932 0.0 1_07G_G7_IDP96865.104_T7S.seq 1840 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 57 ATTCCCC-TCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGT 145 1 6 .......N.......-.N.................N...................................------------------- 75 W S H P Q F E K G T E N L Y F Q S N A M N I N E K T P V G E Query_1 146 GGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGAACATTAATGAGAAAACACCCGTTGGTGAAG 235 0 1 .................................. 34 1 76 -----------------------------............................................................. 136 V R P S Q L L W T Y G P G A L I D L P N L S V V T L G I D R Query_1 236 TTCGCCCGAGCCAATTGCTTTGGACATATGGTCCTGGAGCCCTAATTGATTTGCCCAACTTGTCAGTTGTAACACTTGGGATCGACAGGT 325 0 35 .......................................................................................... 124 1 137 .......................................................................................... 226 W E K N C C Q P I Q E T R L L A A V R K V L G D Q V E S L R Query_1 326 GGGAAAAGAACTGTTGTCAGCCAATTCAGGAAACCCGGTTACTAGCTGCCGTTAGAAAGGTATTAGGTGATCAGGTTGAAAGCTTGCGAA 415 0 125 .......................................................................................... 214 1 227 .......................................................................................... 316 M P P F Q K S E V T D M W S A E A N I G V P V R P F P R W L Query_1 416 TGCCACCATTTCAAAAAAGCGAAGTCACAGATATGTGGTCTGCTGAAGCAAATATAGGGGTACCTGTACGTCCTTTCCCTAGATGGTTGC 505 0 215 .......................................................................................... 304 1 317 .......................................................................................... 406 R C V K C G L L S P Y D A G L F E I K E N R F R P E R T R F Query_1 506 GATGCGTTAAATGTGGGTTACTTTCACCTTACGATGCCGGGCTTTTCGAAATAAAAGAAAATCGATTTAGGCCTGAACGAACTCGCTTTG 595 0 305 .......................................................................................... 394 1 407 .......................................................................................... 496 V H K D C R G S K G D Q P A R D A D A V P A R F L L A C R D Query_1 596 TTCATAAAGACTGCCGGGGTTCTAAGGGCGATCAACCTGCAAGGGATGCTGATGCAGTTCCAGCCCGTTTTCTCTTGGCTTGCCGTGATG 685 0 395 .......................................................................................... 484 1 497 .......................................................................................... 586 G H L D D F P W H Y F V H G G N N P C K G T L R F F E S G A Query_1 686 GTCATCTTGATGATTTTCCGTGGCACTATTTTGTTCATGGTGGTAACAATCCATGCAAAGGAACGCTGCGATTTTTTGAAAGTGGCGCTT 775 0 485 .......................................................................................... 574 1 587 .......................................................................................... 676 S L Q T E N L W V K C D S C E L S R S M A H A F G K A G K E Query_1 776 CATTGCAGACAGAAAATCTTTGGGTAAAATGCGATTCCTGTGAATTATCAAGAAGTATGGCGCATGCATTTGGTAAGGCCGGTAAAGAAA 865 0 575 .......................................................................................... 664 0 1628 ........... 1638 1 677 .......................................................................................... 766 N L P A C R G R H P H L D Y F D A E C D E E P R A V L L G A Query_1 866 ATCTTCCCGCATGTAGAGGACGTCATCCTCATCTGGATTACTTTGATGCTGAATGTGACGAAGAACCTCGTGCAGTACTGCTTGGGGCTA 955 0 665 .......................................................................................... 754 1 767 .......................................................................................... 856 T N S W F P V T L S A L A I P L S N V T P L H Q I I I D Y Query_1 956 CGAATAGCTGGTTTCCTGTAACGCTATCAGCACTAGCGATCCCATTATCAAATGTTAC-TCCATTACATCAGATAAT--CATTGATTACT 1042 0 755 ..........................................................-..................--........... 841 2 1197 ..........TN...T..TC.N......NN. 1167 1 857 ..........................................................T..................--.N......... 944 W D D F S D V E D L S E L K G I L K I L N K R G E A Q G Query_1 1043 GGGATGA-TTTTTCTGATGTAGAA-GAT-CTTT-CTGAACTTAAAGGGATACTAAAAATCC-TCAACAAACG-TGGTGAAGCTC-AAGGT 1125 0 842 .......-................-...-....-...........................-..........-...........-..... 924 2 1166 ......N-.....N.....N....A...T....N.N..C............N....T-..T-..N..N....-........N..C..... 1081 1 945 .......T................-...-....-...........................T..........T..T........-..... 1030 I E K H A A E E I W S A I E T H R S Q D G E D F V G E A D Query_1 1126 ATTGAAAAGCATGCTGCAGAGGAAATA-TGGTCTGCAATTGAAACTCAT-CGCTCACAAGATGGCGAGGATTTTGTCGGTGAGGCTGA-T 1212 0 925 ...........................-.....................-......................................-. 1011 2 1080 ...........................-.....................T......................................-. 993 1 1031 ....................NN.....T.....N...............-NNN....-..N....NN.NN......TC.NNN......T. 1118 I K D P E W N V L I E P T P P T D Y P H F M S K K T R T Query_1 1213 ATTAAAGATCCTGAATGGAATGTAC-TAATAGAACCTACTCCCCCCACTG-ATTATC-CTCATTTTAT--GAGTaaaaaaaCCCGTACTC 1297 0 1012 .........................-........................-......-..........--.................... 1096 2 992 .........................-....................-...-......-.......-..--.................N.. 910 1 1119 .....................N...T.....-N....NN......NN...A......TN.........TG...N.... 1195 P T R F A P Y I E R V L L L E R L R E V N A L L G F T R V E Query_1 1298 CTACGCGGTTTGCGCCATATATTGAGCGAGTTTTACTCTTAGAACGATTACGTGAGGTTAATGCTTTACTTGGTTTTACCCGGGTCGAGG 1387 0 1097 .......................................................................................... 1186 2 909 ...........N.........................N.................................................... 820 A P E E S E D L N E R P Q M A S L S R S K P D W V P A N Q V Query_1 1388 CTCCTGAAGAATCTGAAGATCTCAACGAAAGACCGCAAATGGCCAGCCTGTCACGGAGCAAACCTGATTGGGTACCCGCCAATCAGGTCC 1477 0 1187 .......................................................................................... 1276 2 819 ............N............................................................................. 730 H G E G I F L Q F N E Q A L V A W E S L D A V K Q S D K M L Query_1 1478 ATGGTGAAGGG-ATATTCCTGCAGTTCAATGAACAAGCGCTTGTGGCATGGGAATCTCTGGACGCTGTAAAACAATCGGACAAAATGCTT 1566 0 1277 ...........-.............................................................................. 1365 0 1319 ............ 1308 2 729 ...........G.............................................................................. 640 2 686 ............ 697 R G G H T G W R N A R H L D P E E G Y P G I R Y A M L H T L Query_1 1567 CGCGGTGGGCATACAGGTTGGAGAAATGCTCGTCACTTGGATCCAGAAGAAGGTTATCCCGGCATTCGCTATGCTATGTTGCATACACTT 1656 0 1366 .......................................................................................... 1455 2 639 .......................................................................................... 550 S H L L I R E L A L E C G Y N A A S I R E R I Y A N T T N G Query_1 1657 TCCCATTTACTGATTCGAGAATTGGCTCTGGAATGTGGATATAATGCCGCGAGTATTCGTGAGCGTATCTATGCAAATACAACCAATGGT 1746 0 1456 .......................................................................................... 1545 2 549 .......................................................................................... 460 T P Q A G I L I Y T A A A D S D G T L G G L V D L G K P E N Query_1 1747 ACTCCTCAGGCGGGTATCCTTATTTATACCGCTGCCGCTGATTCAGACGGTACTTTAGGGGGACTGGTTGATCTTGGCAAACCAGAAAAT 1836 0 1546 .......................................................................................... 1635 0 584 ........ 591 2 459 .......................................................................................... 370 1 686 ........ 693 L G R L L E Q A L N R S R I C S S D P L C S E H D P E K D R Query_1 1837 CTTGGACGTTTACTTGAACAAGCATTAAATCGCTCAAGGATCTGCTCATCAGACCCATTATGTTCAGAGCATGATCCAGAGAAGGATCGT 1926 0 1636 .......................................................................................... 1725 0 592 ... 594 2 369 NN..................................................................................A..... 280 1 694 ... 696 S L H G A A C H A C S L V A E T S C E K G N R Y L D R A L L Query_1 1927 TCGCTGCATGGAGCTGCTTGCCATGCCTGTAGCTTAGTTGCAGAAACCTCTTGCGAGAAAGGGAATCGCTATTTAGATAGAGCCCTGCTT 2016 0 1726 .......................................................................................... 1815 2 279 .......................................................................................... 190 V P T L E H T E A A F F K D L L N G * H W K W I T D P N S S Query_1 2017 GTACCAACACTGGAGCATACTGAAGCAGCTTTCTTCAAGGATTTATTAAATGGATAACATTGGAAGTGGATAACGGATCCGAATTCGAGC 2106 0 1816 ...................................................... 1869 2 189 .....................................................................................T.... 100 S V D K L A A A L E H H H H H H * D P A A N K A R K E A E Query_1 2107 TCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT 2194 2 99 ..................................N............N..........N..........N........A..N.NA... 12 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 9691558 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 4,345 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96867.106 (IDP96867.1-1332.F..pMCSG53)
MTENNKLAVSPNAWFAIDRGQSKNPTLALHTVQLKSEQALKHGIADSDWVVVFDTTGHITRIGRILRIRSDLETTTLCFDRILQVEPLIPVGMTSLTLPAKGSFGRIQWKEFIEMLPNTLNTSIAEIPTIEDQTYIRELLQLAVMDDLLGPAAGPNELIVDMGVRDRYLVGKLAPREAAERSSEFPVDPENADDDVGDQIVKSQTTKVHSPKVSGRGEPDVPEEIDAASNQSLVPSSLGMTFCVDGDIDQIELEVRWGRYERSNDHEIYRIRKNKETGVEEQTKVKAWQRFPSGGKITLSLVEGAISPQSLDSSSPEVLIQGTIRPKNENGDRLVTIFLVNTQKEPETNRDAAWVFQPEIIARPVKDAVERSIFRRKPVLDCDGMDPEREALEMIYRNHVEFAVGHGVAVHAEPADNTELATEIRTTVMPQYEVQRTETPGLDPSDRPAMQEMVKSGLLDMQKLATLEVEPLIDALNVLTKDYLDWISEQRASVGIKITGFETQSQIAMDRCKEIHSRLQKGIDTLKLNEKALAAFRFANKAMATQRVRSLYALAKRRGEDTTIESFDIEKNRSWRPFQLAFLLLSIPSLADPNHSDRVQPVNAYADLLWFPTGGGKTEAYLGVAAFTMAIRRMQGNLGGYDSSRGLAVIMRYTLRLLTLQQFQRATALICAMEVLRREALNNGDVSLGLEPFTIGLWVGNKVTPGSTEESHRAIEDARNPGKNHAGTASPAQLTSCPWCGSSIIPGQDVEVKKDKLGGRTFVFCGDKKGRCDFSKGKSSKQAHPGLPVLVVDEEIYHRPPTMMIATVDKFAMMAWRGQVRTLFGRVGLECERHGLLWQGASCTGNHPAAKGQPSTKVRVINPIRPPDLIIQDEFHLISGPLGTMVGLYETAVDELCSWNLDGKSVRPKIIASTATVRKAKEQVNNVFMRQVAVFPPHGIDVEDNFFSVQRPIKEKYGRRYLGVCSPGSSRPAMLIRVYTAFLTAAQELFNHFGEPADPYMTMVGYFNSLRELGGMKRLAEDDVQTRSYRVRMSMVERPALAQRSISNVRELTSRVSSQDIPKYLDNLEVKFKSEFDTTTGKYVTKWQEGDTRAIDIVLATNMLSVGVDVNRLGLMAVNGQPKGTAEYIQATSRVGRSFPGLVCTVLTWARPRDLSHYETFEHYHATFYKHVEAQSVTPFSPRAMDRGLTGALLSLVRLENDEFSPNDGAGKLNMSNQAEISNAIKALANRAGNVAEDNSRKKLAENELKERADEWAKKASMGGRILAYEKRGPEKDKTVSLLDSPGLKPWDNWTVPMSMREVEPGVRLIMNTNHIADDHDWKPRPATKDED
ATGACAGAGAACAATAAGCTAGCTGTATCACCTAATGCATGGTTCGCTATTGATCGTGGCCAATCTAAAAATCCTACTTTGGCCCTTCATACAGTACAGCTAAAAAGTGAACAAGCTCTCAAGCACGGTATTGCTGATAGCGATTGGGTCGTCGTCTTTGACACTACAGGTCATATCACGCGAATCGGACGTATTCTGCGCATTCGCTCTGATCTCGAAACTACCACCTTATGTTTCGACCGAATTTTGCAGGTTGAACCACTCATTCCCGTTGGCATGACTTCACTTACTCTGCCTGCAAAAGGAAGCTTTGGCAGAATTCAGTGGAAAGAATTCATTGAAATGCTTCCAAATACACTAAATACAAGCATTGCTGAAATACCAACGATCGAAGATCAAACTTACATTCGCGAATTACTGCAATTAGCAGTTATGGATGATTTACTCGGGCCGGCAGCTGGCCCTAATGAGCTTATCGTCGATATGGGCGTAAGGGATCGCTATCTTGTTGGTAAGCTTGCTCCACGTGAAGCAGCCGAGCGCAGCTCTGAGTTTCCAGTGGATCCAGAGAATGCTGATGATGATGTTGGTGACCAAATAGTTAAGTCTCAGACAACAAAAGTGCATTCTCCAAAAGTGTCTGGCCGAGGAGAACCCGATGTCCCGGAAGAAATTGATGCCGCCAGCAACCAATCATTGGTACCAAGTAGTCTGGGAATGACTTTTTGTGTCGATGGCGATATTGATCAAATAGAACTAGAAGTTCGTTGGGGACGTTATGAGCGTAGCAATGACCATGAGATCTATCGAATCCGCAAGAACAAGGAGACTGGGGTCGAAGAACAGACCAAAGTCAAAGCTTGGCAGCGTTTTCCTAGTGGCGGGAAGATAACATTATCACTAGTAGAGGGGGCTATTTCCCCTCAATCTTTAGATAGTTCATCTCCTGAAGTACTAATACAGGGCACCATTCGTCCCAAGAATGAAAATGGTGATCGTCTGGTCACTATATTTCTTGTAAATACTCAGAAAGAGCCAGAAACCAACCGAGATGCAGCATGGGTATTCCAACCAGAAATCATTGCTCGCCCAGTGAAGGACGCAGTCGAAAGATCTATTTTTAGGCGCAAGCCAGTTTTGGATTGTGACGGTATGGATCCTGAACGCGAAGCGCTGGAAATGATTTACCGTAACCATGTTGAATTTGCAGTCGGGCATGGTGTTGCAGTACATGCTGAACCTGCAGATAACACTGAACTTGCGACTGAAATTCGTACAACTGTCATGCCACAGTATGAAGTTCAGAGAACAGAGACCCCCGGCCTTGATCCATCAGATCGTCCGGCGATGCAAGAAATGGTCAAAAGTGGTTTACTTGACATGCAGAAACTGGCCACTTTGGAGGTGGAACCACTGATCGATGCATTGAATGTACTAACCAAGGACTATCTGGATTGGATTAGTGAACAACGTGCCAGTGTAGGTATTAAAATTACAGGTTTTGAAACCCAATCTCAGATCGCAATGGATCGTTGTAAGGAGATCCATTCTCGCCTGCAAAAGGGTATTGATACACTTAAGCTTAATGAAAAAGCGCTGGCAGCATTTCGTTTCGCCAACAAAGCAATGGCAACGCAGCGAGTACGAAGTTTATACGCGTTGGCTAAACGCCGTGGTGAAGATACGACTATCGAATCATTTGATATTGAAAAGAATCGTAGCTGGCGTCCTTTTCAGTTGGCATTCTTGTTGCTCTCTATACCTTCGTTGGCAGACCCAAACCATTCCGATCGTGTGCAGCCTGTAAATGCATATGCTGATCTTTTATGGTTCCCCACGGGTGGTGGTAAAACTGAAGCTTATTTAGGCGTTGCGGCTTTTACAATGGCCATACGCCGCATGCAGGGTAATTTGGGGGGGTATGATAGTTCGCGCGGTTTGGCGGTAATAATGCGATATACATTGCGCCTGCTAACATTACAACAATTCCAGCGTGCGACAGCCTTAATTTGTGCTATGGAAGTTTTGCGGCGAGAAGCACTCAATAATGGCGACGTGTCTCTCGGATTAGAACCATTTACTATTGGTCTTTGGGTTGGCAACAAAGTAACGCCTGGTTCGACTGAAGAGAGTCATCGAGCTATTGAAGATGCCCGTAATCCGGGAAAAAACCATGCGGGAACGGCCTCACCTGCACAATTAACTAGTTGTCCATGGTGTGGTTCATCAATTATCCCCGGACAGGATGTTGAAGTTAAAAAGGATAAATTGGGGGGAAGGACGTTTGTTTTTTGCGGTGATAAAAAGGGGCGTTGTGACTTTTCCAAAGGAAAATCAAGTAAACAAGCCCATCCAGGATTGCCTGTATTAGTAGTGGATGAAGAGATCTATCATCGCCCTCCCACAATGATGATTGCTACTGTAGATAAGTTTGCCATGATGGCATGGCGTGGCCAGGTGAGAACGCTTTTCGGCCGTGTAGGATTGGAATGTGAACGACATGGTTTGCTCTGGCAGGGGGCTAGCTGCACAGGAAATCATCCCGCAGCCAAAGGACAACCTTCAACTAAAGTTAGGGTTATCAATCCAATACGACCACCGGACCTTATCATTCAGGATGAATTCCACTTAATTAGTGGACCTTTGGGCACTATGGTTGGATTGTATGAGACTGCTGTTGATGAATTATGTAGTTGGAATCTTGACGGGAAGTCGGTCAGACCAAAGATCATTGCTTCAACTGCAACAGTCCGTAAAGCTAAGGAACAGGTAAATAATGTTTTTATGCGCCAAGTGGCAGTATTTCCACCACATGGGATAGACGTTGAGGATAACTTTTTCTCAGTACAACGACCTATAAAAGAAAAGTATGGACGTCGTTATCTTGGGGTATGCTCGCCTGGCAGTTCAAGGCCCGCAATGCTGATCCGTGTTTACACTGCATTCCTGACCGCAGCACAGGAATTATTCAACCACTTTGGTGAGCCTGCTGACCCTTATATGACAATGGTTGGCTATTTCAACTCGCTACGCGAATTAGGCGGGATGAAGCGACTTGCAGAGGATGACGTACAAACACGTTCTTACCGGGTGCGAATGAGCATGGTGGAAAGACCGGCATTGGCACAACGTAGTATAAGTAATGTTCGTGAATTAACTTCCCGAGTATCCAGTCAGGATATCCCTAAATATCTTGATAATTTAGAGGTTAAGTTTAAATCTGAATTTGATACTACTACTGGTAAGTATGTGACTAAATGGCAGGAGGGGGATACTCGGGCTATAGATATCGTCCTTGCTACTAATATGTTGTCTGTCGGCGTCGATGTTAACCGACTTGGTCTTATGGCAGTAAATGGTCAGCCTAAAGGAACGGCCGAATATATCCAGGCTACCAGTCGTGTAGGGCGTTCATTTCCTGGTTTAGTTTGTACCGTACTAACTTGGGCTCGTCCAAGAGACCTTTCTCATTACGAAACATTCGAACATTACCATGCCACCTTCTACAAGCATGTAGAGGCCCAATCTGTTACTCCTTTTTCTCCTCGAGCGATGGACCGTGGTTTAACAGGTGCGTTATTAAGTCTTGTTCGTCTGGAAAACGACGAATTCAGTCCTAACGATGGGGCTGGTAAGTTGAATATGTCTAATCAAGCCGAGATCAGTAATGCGATTAAAGCACTGGCGAATCGCGCTGGCAATGTTGCAGAAGATAATTCTCGCAAAAAATTGGCAGAGAATGAACTGAAAGAACGTGCCGACGAATGGGCAAAAAAAGCGTCAATGGGCGGACGGATTCTGGCATATGAAAAGCGCGGGCCTGAAAAAGATAAGACTGTCAGCTTATTAGACTCTCCAGGCCTGAAGCCTTGGGACAACTGGACAGTGCCTATGTCAATGCGAGAAGTTGAACCAGGTGTGAGACTTATAATGAACACCAACCATATAGCGGACGATCACGATTGGAAGCCTCGTCCCGCAACTAAGGATGAGGAC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 6,483 total letters Query= IDP96867.106 Length=4401 Score E Sequences producing significant alignments: (Bits) Value 0_wt 7207 0.0 2_12C_C9_IDP96867.106_T7term.seq 1898 0.0 1_09C_C9_IDP96867.106_T7S.seq 1882 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 58 TTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGG 147 0 589 ..A...........G.....-..... 565 0 592 .....A........... 576 1 8 .....................................................................--------------------- 76 1 692 ..A...........G.....-..... 668 1 695 .....A........... 679 S H P Q F E K G T E N L Y F Q S N A M T E N N K L A V S P N Query_1 148 TCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGACAGAGAACAATAAGCTAGCTGTATCACCTAAT 237 0 1 .................................... 36 1 77 ---------------------------............................................................... 139 A W F A I D R G Q S K N P T L A L H T V Q L K S E Q A L K H Query_1 238 GCATGGTTCGCTATTGATCGTGGCCAATCTAAAAATCCTACTTTGGCCCTTCATACAGTACAGCTAAAAAGTGAACAAGCTCTCAAGCAC 327 0 37 .......................................................................................... 126 1 140 .......................................................................................... 229 G I A D S D W V V V F D T T G H I T R I G R I L R I R S D L Query_1 328 GGTATTGCTGATAGCGATTGGGTCGTCGTCTTTGACACTACAGGTCATATCACGCGAATCGGACGTATTCTGCGCATTCGCTCTGATCTC 417 0 127 .......................................................................................... 216 1 230 .......................................................................................... 319 E T T T L C F D R I L Q V E P L I P V G M T S L T L P A K G Query_1 418 GAAACTACCACCTTATGTTTCGACCGAATTTTGCAGGTTGAACCACTCATTCCCGTTGGCATGACTTCACTTACTCTGCCTGCAAAAGGA 507 0 217 .......................................................................................... 306 0 1553 ............ 1564 1 320 .......................................................................................... 409 S F G R I Q W K E F I E M L P N T L N T S I A E I P T I E D Query_1 508 AGCTTTGGCAGAATTCAGTGGAAAGAATTCATTGAAATGCTTCCAAATACACTAAATACAAGCATTGCTGAAATACCAACGATCGAAGAT 597 0 307 .......................................................................................... 396 1 410 .......................................................................................... 499 Q T Y I R E L L Q L A V M D D L L G P A A G P N E L I V D M Query_1 598 CAAACTTACATTCGCGAATTACTGCAATTAGCAGTTATGGATGATTTACTCGGGCCGGCAGCTGGCCCTAATGAGCTTATCGTCGATATG 687 0 397 .......................................................................................... 486 1 500 .......................................................................................... 589 G V R D R Y L V G K L A P R E A A E R S S E F P V D P E N A Query_1 688 GGCGTAAGGGATCGCTATCTTGTTGGTAAGCTTGCTCCACGTGAAGCAGCCGAGCGCAGCTCTGAGTTTCCAGTGGATCCAGAGAATGCT 777 0 487 .......................................................................................... 576 1 590 .......................................................................................... 679 1 76 ...A. 72 1 75 . 75 D D D V G D Q I V K S Q T T K V H S P K V S G R G E P D V P Query_1 778 GATGATGATGTTGGTGACCAAATAGTTAAGTCTCAGACAACAAAAGTGCATTCTCCAAAAGTGTCTGGCCGAGGAGAACCCGATGTCCCG 867 0 577 .......................................................................................... 666 1 680 .......................................................................................... 769 1 71 ..........G.. 59 1 74 ..........A..... 59 E E I D A A S N Q S L V P S S L G M T F C V D G D I D Q I E Query_1 868 GAAGAAATTGATGCCGCCAGCAACCAATCATTGGTACCAAGTAGTCTGGGAATGACTTTTTGTGTCGATGGCGATATTGATCAAATAGAA 957 0 667 .......................................................................................... 756 1 770 .......................................................................................... 859 L E V R W G R Y E R S N D H E I Y R I R K N K E T G V E E Q Query_1 958 CTAGAAGTTCGTTGGGGACGTTATGAGCGTAGCAATGACCATGAGATCTATCGAATCCGCAAGAACAAGGAGACTGGGGTCGAAGAACAG 1047 0 757 .......................................................................................... 846 1 860 .......................................................................................... 949 T K V K A W Q R F P S G G K I T L S L V E G A I S P Q S L D Query_1 1048 ACCAAAGTCAAAGCTTGGCAGCGTTTTCCTAGTGGCGGGAAGATAACATTATCACTAGTAGAGGGGGCTATTTCCCCTCAATCTTTAGAT 1137 0 847 .......................................................................................... 936 1 950 .............................................N................-........................... 1038 S S S P E V L I Q G T I R P K N E N G D R L V T I F L V N Query_1 1138 AGTTCAT-CTCCTGAAGTACTAATACAGGGCACCATTCGTCCCAAGAATGAAAAT-GGTGATCGTCTGGTCACTATATTTCTTGTAAATA 1225 0 937 .......-...............................................-.................................. 1024 0 2373 ............ 2362 1 1039 .-NN...C......-.N..........-................--G........G.............N....N..........-.... 1122 T Q K E P E T N R D A A W V F Q P E I I A R P V K D A V E Query_1 1226 CTCAGAAAG--AGCCAGAAACCAACCGAG-ATGCAGCATGGGTATTCCAACCAGAAATCATTGCTCGCCCAGT-GAAGGACGCAGTCGAA 1311 0 1025 .........--..................-...........................................-................ 1110 1 1123 .CTN.....NN....N.....NC.A....A.........-..NN.......--NN.....-.....CG.....G.......... 1202 R S I F R R K P V L D C D G M D P E R E A L E M I Y R N H V Query_1 1312 AGATCTATTTTTAGGCGCAAGCCAGTTTTGGATTGTGACGGTATGGATCCTGAACGCGAAGCGCTGGAAATGATTTACCGTAACCATGTT 1401 0 1111 .......................................................................................... 1200 E F A V G H G V A V H A E P A D N T E L A T E I R T T V M P Query_1 1402 GAATTTGCAGTCGGGCATGGTGTTGCAGTACATGCTGAACCTGCAGATAACACTGAACTTGCGACTGAAATTCGTACAACTGTCATGCCA 1491 0 1201 .......................................................................................... 1290 Q Y E V Q R T E T P G L D P S D R P A M Q E M V K S G L L D Query_1 1492 CAGTATGAAGTTCAGAGAACAGAGACCCCCGGCCTTGATCCATCAGATCGTCCGGCGATGCAAGAAATGGTCAAAAGTGGTTTACTTGAC 1581 0 1291 .......................................................................................... 1380 M Q K L A T L E V E P L I D A L N V L T K D Y L D W I S E Q Query_1 1582 ATGCAGAAACTGGCCACTTTGGAGGTGGAACCACTGATCGATGCATTGAATGTACTAACCAAGGACTATCTGGATTGGATTAGTGAACAA 1671 0 1381 .......................................................................................... 1470 R A S V G I K I T G F E T Q S Q I A M D R C K E I H S R L Q Query_1 1672 CGTGCCAGTGTAGGTATTAAAATTACAGGTTTTGAAACCCAATCTCAGATCGCAATGGATCGTTGTAAGGAGATCCATTCTCGCCTGCAA 1761 0 1471 .......................................................................................... 1560 0 294 ........ 301 1 397 ........ 404 K G I D T L K L N E K A L A A F R F A N K A M A T Q R V R S Query_1 1762 AAGGGTATTGATACACTTAAGCTTAATGAAAAAGCGCTGGCAGCATTTCGTTTCGCCAACAAAGCAATGGCAACGCAGCGAGTACGAAGT 1851 0 1561 .......................................................................................... 1650 0 302 .... 305 1 405 .... 408 L Y A L A K R R G E D T T I E S F D I E K N R S W R P F Q L Query_1 1852 TTATACGCGTTGGCTAAACGCCGTGGTGAAGATACGACTATCGAATCATTTGATATTGAAAAGAATCGTAGCTGGCGTCCTTTTCAGTTG 1941 0 1651 .......................................................................................... 1740 A F L L L S I P S L A D P N H S D R V Q P V N A Y A D L L W Query_1 1942 GCATTCTTGTTGCTCTCTATACCTTCGTTGGCAGACCCAAACCATTCCGATCGTGTGCAGCCTGTAAATGCATATGCTGATCTTTTATGG 2031 0 1741 .......................................................................................... 1830 F P T G G G K T E A Y L G V A A F T M A I R R M Q G N L G G Query_1 2032 TTCCCCACGGGTGGTGGTAAAACTGAAGCTTATTTAGGCGTTGCGGCTTTTACAATGGCCATACGCCGCATGCAGGGTAATTTggggggg 2121 0 1831 .......................................................................................... 1920 Y D S S R G L A V I M R Y T L R L L T L Q Q F Q R A T A L I Query_1 2122 TATGATAGTTCGCGCGGTTTGGCGGTAATAATGCGATATACATTGCGCCTGCTAACATTACAACAATTCCAGCGTGCGACAGCCTTAATT 2211 0 1921 .......................................................................................... 2010 C A M E V L R R E A L N N G D V S L G L E P F T I G L W V G Query_1 2212 TGTGCTATGGAAGTTTTGCGGCGAGAAGCACTCAATAATGGCGACGTGTCTCTCGGATTAGAACCATTTACTATTGGTCTTTGGGTTGGC 2301 0 2011 .......................................................................................... 2100 N K V T P G S T E E S H R A I E D A R N P G K N H A G T A S Query_1 2302 AACAAAGTAACGCCTGGTTCGACTGAAGAGAGTCATCGAGCTATTGAAGATGCCCGTAATCCGGGAAAAAACCATGCGGGAACGGCCTCA 2391 0 2101 .......................................................................................... 2190 P A Q L T S C P W C G S S I I P G Q D V E V K K D K L G G R Query_1 2392 CCTGCACAATTAACTAGTTGTCCATGGTGTGGTTCATCAATTATCCCCGGACAGGATGTTGAAGTTAAAAAGGATAAATTGGGGGGAAGG 2481 0 2191 .......................................................................................... 2280 T F V F C G D K K G R C D F S K G K S S K Q A H P G L P V L Query_1 2482 ACGTTTGTTTTTTGCGGTGATAAAAAGGGGCGTTGTGACTTTTCCAAAGGAAAATCAAGTAAACAAGCCCATCCAGGATTGCCTGTATTA 2571 0 2281 .......................................................................................... 2370 0 964 ......... 956 1 1067 ........... 1057 V V D E E I Y H R P P T M M I A T V D K F A M M A W R G Q V Query_1 2572 GTAGTGGATGAAGAGATCTATCATCGCCCTCCCACAATGATGATTGCTACTGTAGATAAGTTTGCCATGATGGCATGGCGTGGCCAGGTG 2661 0 2371 .......................................................................................... 2460 0 955 ... 953 1 1056 ... 1054 R T L F G R V G L E C E R H G L L W Q G A S C T G N H P A A Query_1 2662 AGAACGCTTTTCGGCCGTGTAGGATTGGAATGTGAACGACATGGTTTGCTCTGGCAGGGGGCTAGCTGCACAGGAAATCATCCCGCAGCC 2751 0 2461 .......................................................................................... 2550 K G Q P S T K V R V I N P I R P P D L I I Q D E F H L I S G Query_1 2752 AAAGGACAACCTTCAACTAAAGTTAGGGTTATCAATCCAATACGACCACCGGACCTTATCATTCAGGATGAATTCCACTTAATTAGTGGA 2841 0 2551 .......................................................................................... 2640 P L G T M V G L Y E T A V D E L C S W N L D G K S V R P K I Query_1 2842 CCTTTGGGCACTATGGTTGGATTGTATGAGACTGCTGTTGATGAATTATGTAGTTGGAATCTTGACGGGAAGTCGGTCAGACCAAAGATC 2931 0 2641 .......................................................................................... 2730 I A S T A T V R K A K E Q V N N V F M R Q V A V F P P H G I Query_1 2932 ATTGCTTCAACTGCAACAGTCCGTAAAGCTAAGGAACAGGTAAATAATGTTTTTATGCGCCAAGTGGCAGTATTTCCACCACATGGGATA 3021 0 2731 .......................................................................................... 2820 D V E D N F F S V Q R P I K E K Y G R R Y L G V C S P G S S Query_1 3022 GACGTTGAGGATAACTTTTTCTCAGTACAACGACCTATAAAAGAAAAGTATGGACGTCGTTATCTTGGGGTATGCTCGCCTGGCAGTTCA 3111 0 2821 .......................................................................................... 2910 R P A M L I R V Y T A F L T A A Q E L F N H F G E P Query_1 3112 AGGCCCGCAATGCTGATCCGTG--TTTA-CACTG--CATTCCTGACCGCAG--CACAGG-AATTA-TTCAACCACTTTGGTG-AGCCTGC 3191 0 2911 ......................--....-.....--...............--......-.....-................-....... 2990 2 1222 ......NG....G.....NN......N........GCNC....G.....A....NN.NN.......N....... 1149 A D P Y M T M V G Y F N S L R E L G G M K R L A E D D V Q Query_1 3192 TGACCCTTATATGACAATGGTTGGCT-ATTTCAACTC-GCTACG-CGAATTAGGCGGGATGAAGCGACTTGCAGAGGATGACGT-ACAAA 3277 0 2991 ..........................-..........-......-.......................................-..... 3076 2 1148 ..GA..C..NN..NN...........TN.........C......GNN......CG.............................N..... 1059 T R S Y R V R M S M V E R P A L A Q R S I S N V R E L T S Query_1 3278 C-ACGTTCTTACCGGGTGCGAATG-AGCATGGTGGAAAGACCGGCATTGGCACAACGTAGTATAAGTAATGTTCGTGAATTAACTTCCCG 3365 0 3077 .-......................-................................................................. 3164 2 1058 .C....N-..........N.....N......................N.............N......-..................... 971 R V S S Q D I P K Y L D N L E V K F K S E F D T T T G K Y V Query_1 3366 AGTATCCAGTCAGGATATCCCTAAA-TATCTTGATAATTTAGAGGTTAA-GTTTAAATCTGAATTTGATACTACTACTGGTAAGTATGTG 3453 0 3165 .........................-.......................-........................................ 3252 2 970 .........................N.......................A........................................ 881 T K W Q E G D T R A I D I V L A T N M L S V G V D V N R L G Query_1 3454 ACTAAATGGCAGGAGGGGGATACTCGGGCTATAGATATCGTCCTTGCTACTAATATGTTGTCTGTCGGCGTCGATGTTAACCGACTTGGT 3543 0 3253 .......................................................................................... 3342 2 880 .......................................................................................... 791 L M A V N G Q P K G T A E Y I Q A T S R V G R S F P G L V Query_1 3544 CTTATGGCAGTAAATGGTCAGCCTAAAGGAACGGCCGAATATATCCAGG-CTACCAGTCGTGTAGGGCGTTCATTTCCTGGTTTAGTTTG 3632 0 3343 .................................................-........................................ 3431 2 790 .................................................G........................................ 701 C T V L T W A R P R D L S H Y E T F E H Y H A T F Y K H V E Query_1 3633 TACCGTACTAACTTGGGCTCGTCCAAGAGACCTTTCTCATTACGAAA-CATTCGAACATTACCATGCCACCTTCTACAAGCATGTAGAGG 3721 0 3432 ...............................................-.......................................... 3520 2 700 ...............................................A.......................................... 611 A Q S V T P F S P R A M D R G L T G A L L S L V R L E N D E Query_1 3722 CCCAATCTGTTACTCCTTTTTCTCCTCGAGCGATGGACCGTGGTTTAACAGGTGCGTTATTAAGTCTTGTTCGTCTGGAAAACGACGAAT 3811 0 3521 .......................................................................................... 3610 2 610 ................C......................................................................... 521 F S P N D G A G K L N M S N Q A E I S N A I K A L A N R A G Query_1 3812 TCAGTCCTAACGATGGGGCTGGTAAGTTGAATATGTCTAATCAAGCCGAGATCAGTAATGCGATTAAAGCACTGGCGAATCGCGCTGGCA 3901 0 3611 .......................................................................................... 3700 2 520 .......................................................................................... 431 N V A E D N S R K K L A E N E L K E R A D E W A K K A S M G Query_1 3902 ATGTTGCAGAAGATAATTCTCGCAAAAAATTGGCAGAGAATGAACTGAAAGAACGTGCCGACGAATGGGCaaaaaaaGCGTCAATGGGCG 3991 0 3701 .......................................................................................... 3790 2 430 .......................................................................................... 341 G R I L A Y E K R G P E K D K T V S L L D S P G L K P W D N Query_1 3992 GACGGATTCTGGCATATGAAAAGCGCGGGCCTGAAAAAGATAAGACTGTCAGCTTATTAGACTCTCCAGGCCTGAAGCCTTGGGACAACT 4081 0 3791 .......................................................................................... 3880 2 340 ......................................................................................G... 251 W T V P M S M R E V E P G V R L I M N T N H I A D D H D W K Query_1 4082 GGACAGTGCCTATGTCAATGCGAGAAGTTGAACCAGGTGTGAGACTTATAATGAACACCAACCATATAGCGGACGATCACGATTGGAAGC 4171 0 3881 .......................................................................................... 3970 2 250 .......................................................................................... 161 P R P A T K D E D * H W K W I T D P N S S S V D K L A A A L Query_1 4172 CTCGTCCCGCAACTAAGGATGAGGACTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCG 4261 0 3971 .......................... 3996 2 160 ............................................................................N............. 71 E H H H H H H * D P A A N K A R K E Query_1 4262 AGcaccaccaccaccaccacTGAGATCCGGCTGCTAA--CAAAGCCCGAAAGGAA 4314 2 70 .....................................ACA............... 16 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 28178307 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 6,483 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96870.107 (IDP96870.32-220.P(220)..pMCSG53)
HAKNIERAHQAANDFLDEMTSLPMRVMLLETEMVRGLVRAESVISYKNKNEFVCELLRLMDNSNKIRLSFFNSYERVVRRGINIKPSQKHMKLEKSVIEFTEHVSKILQNAAEAISRSTTTEEYREPFRTLAGARIVITKNKNTLNEACAATKDISFDDYFSFPSAYGWFRHKWDSLIRPFLFKPFKNM
CATGCCAAAAATATTGAGCGAGCGCATCAGGCGGCAAATGATTTTCTTGACGAAATGACATCATTACCAATGAGAGTAATGTTACTAGAAACAGAGATGGTCAGAGGGTTAGTTAGAGCTGAATCAGTTATTAGTTATAAAAATAAAAATGAATTTGTGTGTGAGTTGCTGCGTCTTATGGATAATTCGAATAAGATCAGACTTTCGTTTTTTAATTCCTATGAAAGAGTTGTTCGAAGGGGGATTAACATCAAACCTTCCCAAAAACATATGAAACTCGAAAAATCTGTTATTGAGTTTACTGAACATGTTTCAAAAATTCTCCAGAATGCAGCAGAGGCTATTAGTAGATCGACAACAACAGAAGAATATAGAGAGCCATTCAGAACGTTAGCTGGTGCTAGAATTGTTATAACCAAAAATAAAAATACACTCAATGAAGCCTGTGCTGCTACAAAGGATATTTCGTTTGATGACTATTTTTCCTTTCCTTCAGCATATGGTTGGTTCAGGCATAAATGGGATAGTTTGATTCGACCTTTTTTATTTAAACCGTTTAAAAATATG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,013 total letters Query= IDP96870.107 Length=972 Score E Sequences producing significant alignments: (Bits) Value 1_07H_H7_IDP96870.107_T7S.seq 1458 0.0 2_10H_H7_IDP96870.107_T7term.seq 1416 0.0 0_wt 1023 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAA 90 1 11 .........-................... 38 2 843 .......................................................................................... 754 2 776 ...G...........T.T.C.C.C...........A... 814 E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E N Query_1 91 GAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAAC 180 1 39 ....................................------------------------------------------------...... 80 2 753 ....................................------------------------------------------------...... 712 L Y F Q S N A H A K N I E R A H Q A A N D F L D E M T S L P Query_1 181 CTGTACTTCCAATCCAATGCCCATGCCAAAAATATTGAGCGAGCGCATCAGGCGGCAAATGATTTTCTTGACGAAATGACATCATTACCA 270 1 81 .......................................................................................... 170 2 711 .......................................................................................... 622 0 1 ..................................................................... 69 M R V M L L E T E M V R G L V R A E S V I S Y K N K N E F V Query_1 271 ATGAGAGTAATGTTACTAGAAACAGAGATGGTCAGAGGGTTAGTTAGAGCTGAATCAGTTATTAGTTATAAAAATAAAAATGAATTTGTG 360 1 171 .......................................................................................... 260 1 519 ............ 530 2 621 .......................................................................................... 532 2 273 ............ 262 0 70 .......................................................................................... 159 0 418 ............ 429 C E L L R L M D N S N K I R L S F F N S Y E R V V R R G I N Query_1 361 TGTGAGTTGCTGCGTCTTATGGATAATTCGAATAAGATCAGACTTTCGTTTTTTAATTCCTATGAAAGAGTTGTTCGAAGGGGGATTAAC 450 1 261 .......................................................................................... 350 2 531 .......................................................................................... 442 0 160 .......................................................................................... 249 I K P S Q K H M K L E K S V I E F T E H V S K I L Q N A A E Query_1 451 ATCAAACCTTCCCAAAAACATATGAAACTCGAAAAATCTGTTATTGAGTTTACTGAACATGTTTCAAAAATTCTCCAGAATGCAGCAGAG 540 1 351 .......................................................................................... 440 2 441 .......................................................................................... 352 0 250 .......................................................................................... 339 A I S R S T T T E E Y R E P F R T L A G A R I V I T K N K N Query_1 541 GCTATTAGTAGATCGACAACAACAGAAGAATATAGAGAGCCATTCAGAACGTTAGCTGGTGCTAGAATTGTTATAACCAAAAATAAAAAT 630 1 441 .......................................................................................... 530 1 240 ............ 251 2 351 .......................................................................................... 262 2 552 ............ 541 0 340 .......................................................................................... 429 0 139 ............ 150 T L N E A C A A T K D I S F D D Y F S F P S A Y G W F R H K Query_1 631 ACACTCAATGAAGCCTGTGCTGCTACAAAGGATATTTCGTTTGATGACTATTTTTCCTTTCCTTCAGCATATGGTTGGTTCAGGCATAAA 720 1 531 .......................................................................................... 620 2 261 .......................................................................................... 172 0 430 .......................................................................................... 519 W D S L I R P F L F K P F K N M * H W K W I T D P N S S S V Query_1 721 TGGGATAGTTTGATTCGACCTTTTTTATTTAAACCGTTTAAAAATATGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTC 810 1 621 .......................................................................................... 710 2 171 .......................................................................................... 82 0 520 ................................................ 567 D K L A A A L E H H H H H H * D P A A N K A R K E A E L A A Query_1 811 GACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCT 900 1 711 .......................................................................................... 800 2 81 .......................................................................A.... 6 A T A E Q * L A * P L G A S K R V L R G F L L K Query_1 901 GCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAA 972 1 801 ........................................................................ 872 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2840376 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 3,013 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96874.104 (IDP96874.1-156.F..pMCSG53)
MMTLRTFPMLHDLSDSLFADRFNRIDRLFSQLTGSTPLPSTPSYNIRRMGDNRYELTLSVPGWKESELEIETVGGQLNISGKREEEKTENGEEGWIHRGISRSDFRASYSLPEHVKVTGASLENGLLAIELHQDIPEEEKPQRIAINNNPAIEHKP
ATGATGACTCTCAGAACTTTTCCCATGTTGCACGATCTCTCCGACTCTCTGTTTGCGGACCGTTTTAACCGCATCGATCGCCTTTTTAGCCAGCTAACCGGCAGTACACCGTTGCCCTCCACGCCTTCTTACAATATCCGGCGGATGGGTGATAACCGCTATGAACTGACGCTCAGTGTGCCGGGCTGGAAAGAGAGCGAACTGGAAATTGAAACCGTCGGCGGTCAGCTGAACATTAGCGGTAAACGTGAAGAGGAGAAAACCGAAAACGGCGAAGAAGGATGGATCCATCGGGGTATCAGCCGCAGCGACTTCCGGGCCAGCTATAGTCTGCCGGAGCATGTGAAAGTGACCGGCGCTTCGCTGGAAAACGGCTTGCTGGCGATTGAATTACACCAGGATATCCCGGAAGAAGAGAAACCGCAGCGCATCGCCATCAACAACAACCCGGCGATTGAACACAAGCCG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,923 total letters Query= IDP96874.104 Length=873 Score E Sequences producing significant alignments: (Bits) Value 1_08A_A8_IDP96874.104_T7S.seq 1281 0.0 2_11A_A8_IDP96874.104_T7term.seq 1236 0.0 0_wt 845 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTC-TAGAAATAATTTTGTTTAACTTTA 89 1 9 ........N........................ 41 2 754 .................................................................-........................ 666 2 687 ...G...........T.T.C.C.C...........A-... 725 * E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E Query_1 90 AGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAA 179 1 42 .....................................------------------------------------------------..... 83 2 665 .....................................------------------------------------------------..... 624 N L Y F Q S N A M M T L R T F P M L H D L S D S L F A D R F Query_1 180 CCTGTACTTCCAATCCAATGCCATGATGACTCTCAGAACTTTTCCCATGTTGCACGATCTCTCCGACTCTCTGTTTGCGGACCGTTTTAA 269 1 84 .......................................................................................... 173 2 623 .......................................................................................... 534 0 1 .................................................................... 68 N R I D R L F S Q L T G S T P L P S T P S Y N I R R M G D N Query_1 270 CCGCATCGATCGCCTTTTTAGCCAGCTAACCGGCAGTACACCGTTGCCCTCCACGCCTTCTTACAATATCCGGCGGATGGGTGATAACCG 359 1 174 .......................................................................................... 263 2 533 .......................................................................................... 444 0 69 .......................................................................................... 158 R Y E L T L S V P G W K E S E L E I E T V G G Q L N I S G K Query_1 360 CTATGAACTGACGCTCAGTGTGCCGGGCTGGAAAGAGAGCGAACTGGAAATTGAAACCGTCGGCGGTCAGCTGAACATTAGCGGTAAACG 449 1 264 .......................................................................................... 353 2 443 .......................................................................................... 354 0 159 .......................................................................................... 248 R E E E K T E N G E E G W I H R G I S R S D F R A S Y S L P Query_1 450 TGAAGAGGAGAAAACCGAAAACGGCGAAGAAGGATGGATCCATCGGGGTATCAGCCGCAGCGACTTCCGGGCCAGCTATAGTCTGCCGGA 539 1 354 .......................................................................................... 443 1 397 ............ 386 2 353 .......................................................................................... 264 2 310 ............ 321 0 249 .......................................................................................... 338 0 292 ............ 281 E H V K V T G A S L E N G L L A I E L H Q D I P E E E K P Q Query_1 540 GCATGTGAAAGTGACCGGCGCTTCGCTGGAAAACGGCTTGCTGGCGATTGAATTACACCAGGATATCCCGGAAGAAGAGAAACCGCAGCG 629 1 444 .......................................................................................... 533 2 263 .......................................................................................... 174 0 339 .......................................................................................... 428 R I A I N N N P A I E H K P * H W K W I T D P N S S S V D K Query_1 630 CATCGCCATCAACAACAACCCGGCGATTGAACACAAGCCGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCT 719 1 534 .......................................................................................... 623 2 173 .......................................................................................... 84 0 429 ........................................ 468 L A A A L E H H H H H H * D P A A N K A R K E A E L A A A T Query_1 720 TGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAAC-AAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCG 808 1 624 ....................................................A..................................... 713 2 83 ....................................................-...........A... 17 A E Q * L A * P L G A S K R V L R G F L L K Query_1 809 CTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAA 873 1 714 ................................................................. 778 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2469324 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 2,923 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96876.106 (IDP96876.1-74.F..pMCSG53)
MGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW
ATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGTGCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATACCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,509 total letters Query= IDP96876.106 Length=627 Score E Sequences producing significant alignments: (Bits) Value 1_09B_B9_IDP96876.106_T7S.seq 843 0.0 2_12B_B9_IDP96876.106_T7term.seq 791 0.0 0_wt 401 2e-115 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAA 90 1 7 ..T.N...T........................... 42 2 501 .......................................................................................... 412 2 434 ...G...........T.T.C.C.C...........A... 472 E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E N Query_1 91 GAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAAC 180 1 43 ....................................------------------------------------------------...... 84 2 411 ....................................------------------------------------------------...... 370 L Y F Q S N A M G R I L L D L S D D V I Q R L D D L K V Q R Query_1 181 CTGTACTTCCAATCCAATGCCATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGTGCAGCGT 270 1 85 .......................................................................................... 174 2 369 .......................................................................................... 280 0 1 ..................................................................... 69 N I P R A E L L R E A V E Q Y L E K Q D R A K D T I S S A L Query_1 271 AACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATACCATCTCCAGTGCGCTG 360 1 175 .......................................................................................... 264 2 279 .......................................................................................... 190 0 70 .......................................................................................... 159 G L W Q D C E E D G M E Y Q R Q L R K E W * H W K W I T D P Query_1 361 GGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGGTAACATTGGAAGTGGATAACGGATCCG 450 1 265 .......................................................................................... 354 2 189 .......................................................................................... 100 0 160 ............................................................... 222 N S S S V D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 451 AATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAA 540 1 355 .......................................................................................... 444 2 99 ..................................................................................N...A... 10 A E L A A A T A E Q * L A * P L G A S K R V L R G F L L K Query_1 541 GCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAA 627 1 445 ....................................................................................... 531 2 9 . 9 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 1507968 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 2,509 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96877.104 (IDP96877.3-382.P(740)..pMCSG53)
KILIMTPDIEGPVRNGGIGTAFTALATTLAKKGYDVDVLYTCGDYSESSVSKFSDWSRIYSTFGINLLRTGLIKEINIDAPYFRRKSYSIYLWLKENNIYDTVISCEWQADLYYTLLSKKNGTDFENTKFIVNTHSSTLWADEGNYQLPYDQNHLELYYMEKMVVEMADEVVSPSQYLIDWMLSKHWNVPEERHVILNCEPFQGFVTRDDVTVKINEKPASGVELVFFGRLETRKGLDIFLRALRKLSDEDKESISGVTFLGKNVTMGKTDSFTYIMNQTKNLGLAVNVISDYDRTNANEYIKRKNVLVIIPSLVENSPYTVYECLINNVNFLASNVGGIPELIPQEHHAEVLFIPTPVDLYGKIHYRLKNINIKPGLAE
AAAATTCTTATAATGACGCCGGACATTGAGGGGCCTGTCCGTAACGGCGGTATTGGTACTGCTTTCACTGCTCTTGCCACTACTTTGGCAAAAAAGGGGTATGATGTTGATGTATTGTATACATGTGGCGACTATTCTGAATCATCTGTATCGAAATTTAGCGACTGGTCACGTATTTATAGTACCTTTGGTATCAATCTGCTAAGAACCGGACTGATAAAAGAGATTAATATTGATGCACCGTATTTTAGAAGGAAAAGTTATTCAATTTATCTCTGGTTGAAAGAAAATAACATCTATGACACTGTTATTTCTTGTGAGTGGCAGGCAGATCTTTATTACACTTTATTAAGCAAAAAGAATGGAACGGATTTTGAAAATACAAAGTTCATTGTAAATACTCACAGTTCAACGTTATGGGCTGATGAAGGTAATTACCAGCTTCCATATGATCAGAACCATCTTGAACTCTATTATATGGAGAAAATGGTGGTTGAAATGGCGGATGAAGTTGTTAGTCCGTCTCAGTATTTAATTGATTGGATGTTGAGTAAGCACTGGAATGTTCCTGAAGAACGTCATGTAATTTTAAATTGCGAGCCATTTCAAGGGTTTGTGACGAGAGATGATGTTACAGTTAAAATAAATGAAAAGCCAGCTTCTGGCGTTGAGCTTGTATTTTTCGGCCGCCTTGAAACCCGTAAAGGACTTGACATATTCCTGCGTGCATTAAGAAAACTATCTGATGAAGATAAAGAGAGCATTTCTGGAGTAACCTTCCTCGGAAAAAATGTCACTATGGGGAAAACTGATTCATTTACTTATATTATGAATCAGACTAAAAATTTGGGACTCGCAGTTAATGTAATCAGCGACTATGATCGTACCAACGCTAATGAATATATAAAAAGAAAAAATGTATTAGTCATCATTCCATCACTTGTAGAAAACTCACCCTATACTGTTTATGAATGCTTGATTAATAACGTTAATTTCCTCGCTTCAAACGTTGGTGGAATTCCAGAGCTTATTCCGCAAGAGCATCATGCGGAAGTTCTATTTATTCCTACACCTGTCGATTTATACGGGAAAATCCACTATCGCTTAAAAAATATAAATATAAAACCAGGGCTTGCTGAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,437 total letters Query= IDP96877.104 Length=1545 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2057 0.0 1_02C_C2_IDP96877.104_T7S.seq 1752 0.0 2_08C_C2_IDP96877.104_T7term.seq 1707 0.0 Q F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 55 CAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTG 144 1 14 ................-.......................................................------------------ 84 W S H P Q F E K G T E N L Y F Q S N A K I L I M T P D I E G Query_1 145 TGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCAAAATTCTTATAATGACGCCGGACATTGAGGGG 234 0 1 ................................. 33 1 85 ------------------------------............................................................ 144 P V R N G G I G T A F T A L A T T L A K K G Y D V D V L Y T Query_1 235 CCTGTCCGTAACGGCGGTATTGGTACTGCTTTCACTGCTCTTGCCACTACTTTGGCAAAAAAGGGGTATGATGTTGATGTATTGTATACA 324 0 34 .......................................................................................... 123 1 145 .......................................................................................... 234 C G D Y S E S S V S K F S D W S R I Y S T F G I N L L R T G Query_1 325 TGTGGCGACTATTCTGAATCATCTGTATCGAAATTTAGCGACTGGTCACGTATTTATAGTACCTTTGGTATCAATCTGCTAAGAACCGGA 414 0 124 .......................................................................................... 213 1 235 .......................................................................................... 324 2 1067 ...... 1062 L I K E I N I D A P Y F R R K S Y S I Y L W L K E N N I Query_1 415 C-TGA-TAAAAGA-GATTAATATTGATGCACC-GTATTTTAGAAGGAAAAGTTATTCAATTTATCTCTGGTTGAAAGAAAA--TAACATC 498 0 214 .-...-.......-..................-................................................--....... 297 1 325 .-...-.......-..................-................................................--....... 408 2 1061 NN...N.......A........N.........C......-............A..-.....-.........GA........ATN.C.... 975 Y D T V I S C E W Q A D L Y Y T L L S K K N G T D F E N T Query_1 499 TATGACACTGTTATTTCTTGTGAGTGGCAGGCA-GATCTTTATTACACTTTATTAAGCAAAAAGAATGGAACGGATTTTGAAAATACAAA 587 0 298 .................................-........................................................ 386 1 409 .................................-........................................................ 497 1 794 .......... 785 2 974 .........N........GN.N...........A......NN.-..................G--.............-........... 889 K F I V N T H S S T L W A D E G N Y Q L P Y D Q N H L E L Y Query_1 588 GTTCATTGTAAATACTCACAGTTCAACGTTATGGGCTGATGAAGGTAATTACCAGCTTCCATATGATCAGAACCATCTTGAACTCTATTA 677 0 387 .......................................................................................... 476 1 498 .......................................................................................... 587 1 784 .C... 780 2 888 ...........-.............................................................................. 800 Y M E K M V V E M A D E V V S P S Q Y L I D W M L S K H W N Query_1 678 TATGGAGAAAATGGTGGTTGAAATGGCGGATGAAGTTGTTAGTCCGTCTCAGTATTTAATTGATTGGATGTTGAGTAA-GCACTGGAATG 766 0 477 ..............................................................................-........... 565 1 588 ..............................................................................-........... 676 2 799 ..................-...........................................................A........... 711 V P E E R H V I L N C E P F Q G F V T R D D V T V K I N E K Query_1 767 TTCCTGAAGAACGTCATGTAATTTTAAATTGCGAGCCATTTCAAGGGTTTGTGACGAGAGATGATGTTACAGTTAAAATAAATGAAAAGC 856 0 566 .......................................................................................... 655 1 677 .......................................................................................... 766 2 710 ........................N................................................................. 621 P A S G V E L V F F G R L E T R K G L D I F L R A L R K L Query_1 857 CAGCTTCTGGCGTTGAGC-TTGTA-TTTTTCGGCCGCCTTGAAACCCGTAAAGGACTTGACATATTCCTGCGTGCATTAAGAAAACTATC 944 0 656 ..................-.....-................................................................. 743 1 767 ..................T.....T................................................................. 856 2 620 ..................-.....-................................................................. 533 S D E D K E S I S G V T F L G K N V T M G K T D S F T Y I M Query_1 945 TGATGAAGATAAAGAGAGCATTTCTGGAGTAACCTT-CCTCGGAAAAAATGTCACTATGGGGAAAACTGATTCATTTACTTATATT-ATG 1032 0 744 ....................................-.................................................-... 831 1 857 ....................................N.....................-...........................N... 945 2 532 ....................................-.................................................-... 445 N Q T K N L G L A V N V I S D Y D R T N A N E Y I K R K N Query_1 1033 AATCAGACTAAAAATTTGGGACTCGCAGTTAATGTAATCAGCGACTATGATCGTACCAACGCTAATGAATA-TATaaaaagaaaaaaTGT 1121 0 832 .......................................................................-.................. 920 1 946 .............N....................-N...................................N.......GA......... 1034 2 444 .......................................................................-.................. 356 V L V I I P S L V E N S P Y T V Y E C L I N N V N F L A Query_1 1122 ATTAGTCAT-CATTCCATCACTTGTAG-AAAACT-CACCC-TAT-ACTGTTTATGAATGCTT-GATTAAT-AACGTTAATTT-CCTCGCT 1203 0 921 .........-.................-......-.....-...-.................-.......-...........-....... 1002 1 1035 .........N......N....C..N..A......C.....N...A...............N.G.......A....G......C....N.C 1124 2 355 .........-.................-......-.....-...-.................-.......-...........-....... 274 S N V G G I P E L I P Q E H H A E V L F I P T P V D L Y G K Query_1 1204 TCAAACGTTGGTGGAATTCCAGAGCTTATTCCGCAAGAGCATCATGCGGAAGTTCTATTTATTCCTACACCTGTCGATTTATACGGGAAA 1293 0 1003 .......................................................................................... 1092 1 1125 .N.... 1130 2 273 .......................................................................................... 184 I H Y R L K N I N I K P G L A E * H W K W I T D P N S S S V Query_1 1294 ATCCACTATCGCTTAAAAAATATAAATATAAAACCAGGGCTTGCTGAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTC 1383 0 1093 ................................................ 1140 2 183 .......................................................................................... 94 D K L A A A L E H H H H H H * D P A A N K A R Query_1 1384 GACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAAC-AAAGCCCGAAA 1454 2 93 ............................................................A........... 22 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5181781 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 3,437 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96881.104 (IDP96881.34-222.P(222)..pMCSG53)
KDYQAGKNFTVIHSTVKQPPPLVEFFSFYCGPCYAFAERINVDTAIRKRLPDDMKLEKYHVSQMGPLGPALTEAWAVAQYAGVDGKVEKLLFEGLQVKRDIKTAADIVKVFNQLGITSEKYAEMQSNFMVKALIARQDNLVEKMKVHGTPSFYVSGKYHINNASLAQDDYDTYAEDMANLVLFLLNKPL
AAAGATTATCAGGCAGGGAAAAACTTCACTGTTATTCATTCTACTGTTAAGCAACCGCCGCCACTGGTTGAGTTTTTTTCCTTCTACTGCGGACCTTGTTATGCGTTTGCAGAGCGCATCAATGTTGATACAGCTATCAGAAAGCGCCTGCCCGACGATATGAAACTGGAAAAATACCATGTCAGCCAGATGGGGCCTTTAGGACCAGCATTAACAGAAGCATGGGCCGTAGCACAATATGCTGGGGTAGATGGCAAGGTGGAAAAGTTATTGTTTGAGGGTTTACAGGTTAAGCGTGATATTAAAACGGCAGCTGATATTGTTAAGGTGTTTAATCAACTTGGCATTACGTCAGAGAAATATGCCGAAATGCAAAGTAACTTTATGGTAAAAGCTTTAATTGCCAGACAGGATAATCTGGTCGAAAAAATGAAAGTGCATGGCACGCCTTCTTTTTATGTTTCAGGAAAATATCATATTAACAATGCATCACTCGCGCAAGATGACTATGATACCTATGCAGAGGATATGGCTAATCTGGTTTTATTTTTGCTTAACAAGCCCCTG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,022 total letters Query= IDP96881.104 Length=972 Score E Sequences producing significant alignments: (Bits) Value 1_08B_B8_IDP96881.104_T7S.seq 1467 0.0 2_11B_B8_IDP96881.104_T7term.seq 1420 0.0 0_wt 1023 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAA 90 1 8 ..............-................... 40 2 852 .......................................................................................... 763 2 785 ...G...........T.T.C.C.C...........A... 823 E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E N Query_1 91 GAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAAC 180 1 41 ....................................------------------------------------------------...... 82 2 762 ....................................------------------------------------------------...... 721 L Y F Q S N A K D Y Q A G K N F T V I H S T V K Q P P P L V Query_1 181 CTGTACTTCCAATCCAATGCCAAAGATTATCAGGCAGGGAAAAACTTCACTGTTATTCATTCTACTGTTAAGCAACCGCCGCCACTGGTT 270 1 83 .......................................................................................... 172 2 720 .......................................................................................... 631 0 1 ..................................................................... 69 E F F S F Y C G P C Y A F A E R I N V D T A I R K R L P D D Query_1 271 GAGtttttttCCTTCTACTGCGGACCTTGTTATGCGTTTGCAGAGCGCATCAATGTTGATACAGCTATCAGAAAGCGCCTGCCCGACGAT 360 1 173 .......................................................................................... 262 2 630 .......................................................................................... 541 0 70 .......................................................................................... 159 M K L E K Y H V S Q M G P L G P A L T E A W A V A Q Y A G V Query_1 361 ATGAAACTGGAAAAATACCATGTCAGCCAGATGGGGCCTTTAGGACCAGCATTAACAGAAGCATGGGCCGTAGCACAATATGCTGGGGTA 450 1 263 .......................................................................................... 352 2 540 .......................................................................................... 451 0 160 .......................................................................................... 249 D G K V E K L L F E G L Q V K R D I K T A A D I V K V F N Q Query_1 451 GATGGCAAGGTGGAAAAGTTATTGTTTGAGGGTTTACAGGTTAAGCGTGATATTAAAACGGCAGCTGATATTGTTAAGGTGTTTAATCAA 540 1 353 .......................................................................................... 442 2 450 .......................................................................................... 361 0 250 .......................................................................................... 339 L G I T S E K Y A E M Q S N F M V K A L I A R Q D N L V E K Query_1 541 CTTGGCATTACGTCAGAGAAATATGCCGAAATGCAAAGTAACTTTATGGTAAAAGCTTTAATTGCCAGACAGGATAATCTGGTCGAAAAA 630 1 443 .......................................................................................... 532 2 360 .......................................................................................... 271 0 340 .......................................................................................... 429 M K V H G T P S F Y V S G K Y H I N N A S L A Q D D Y D T Y Query_1 631 ATGAAAGTGCATGGCACGCCTTCTTTTTATGTTTCAGGAAAATATCATATTAACAATGCATCACTCGCGCAAGATGACTATGATACCTAT 720 1 533 .......................................................................................... 622 2 270 .......................................................................................... 181 0 430 .......................................................................................... 519 A E D M A N L V L F L L N K P L * H W K W I T D P N S S S V Query_1 721 GCAGAGGATATGGCTAATCTGGTTTTATTTTTGCTTAACAAGCCCCTGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTC 810 1 623 .......................................................................................... 712 2 180 .......................................................................................... 91 0 520 ................................................ 567 D K L A A A L E H H H H H H * D P A A N K A R K E A E L A A Query_1 811 GACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCT 900 1 713 .......................................................................................... 802 2 90 ............................................................................ 15 A T A E Q * L A * P L G A S K R V L R G F L L K Query_1 901 GCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAA 972 1 803 ........................................................................ 874 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2848989 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 3,022 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96885.107 (IDP96885.32-441.P(441)..pMCSG53)
SSFDEHLDASAGGGGGSSIDAVMVDPGAVVNNYNRQQQQQASARRAAEQREKQAQQQAEELREKQAAEQERLKQLEQERLQAQEAAKEAKEQQKQAEEAAAKAAAAAKAKADAQAKEAQEAAAKAAAEAKAKADAQAKAAEQAAAKAAADAKKQAEAAAAKAAAEAKKQAEAEAAKAAAEAQKKAEAAAAKKAQQEAEKKAQQEAAKQAAAEKAAAEKAAEKAAAQKAAAEKAAAEKAAAAEKAAAAKAAAAEKAAADKAAKAAAAKAAAAKKAAAAKEADGVDNLLGDLSSGKNAPKTGGGAKGNNASAAGSGNTKNSASGADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGLLLNIQSEGGDPALCQAALAAARQAKFPKPPSQAVYEVFKNAPLDFKPQ
AGTTCGTTTGACGAGCATCTGGATGCCTCTGCCGGCGGCGGCGGCGGATCGTCGATTGATGCCGTCATGGTCGATCCGGGGGCGGTGGTAAATAACTATAACCGTCAGCAACAGCAGCAGGCCAGCGCACGTCGCGCCGCTGAACAGCGTGAAAAACAGGCGCAGCAGCAGGCGGAAGAGTTACGTGAGAAACAGGCGGCGGAACAGGAACGGCTGAAACAGCTCGAACAGGAGCGGCTGCAGGCGCAGGAAGCGGCGAAAGAAGCGAAGGAGCAGCAGAAGCAGGCTGAAGAAGCGGCTGCCAAGGCCGCCGCGGCGGCAAAAGCCAAAGCGGACGCACAGGCAAAAGAAGCGCAGGAAGCCGCTGCCAAAGCGGCCGCCGAGGCGAAAGCGAAGGCGGATGCCCAGGCGAAAGCGGCAGAACAGGCGGCGGCCAAGGCGGCTGCTGACGCGAAAAAGCAGGCCGAAGCCGCTGCAGCGAAAGCCGCTGCCGAGGCGAAGAAACAGGCGGAAGCTGAAGCGGCGAAAGCTGCGGCCGAGGCGCAGAAGAAAGCGGAAGCGGCGGCTGCGAAGAAAGCGCAACAGGAAGCGGAGAAAAAAGCCCAGCAGGAAGCGGCTAAGCAGGCGGCAGCTGAAAAAGCGGCTGCCGAAAAAGCCGCTGAGAAAGCCGCCGCGCAAAAAGCGGCCGCTGAGAAGGCCGCCGCCGAGAAAGCCGCAGCCGCTGAAAAAGCGGCGGCAGCGAAAGCGGCTGCAGCAGAGAAGGCTGCAGCTGATAAAGCGGCCAAAGCGGCAGCAGCAAAAGCCGCGGCGGCGAAGAAAGCGGCGGCTGCGAAAGAAGCGGACGGCGTTGACAACCTGCTCGGCGATCTGAGTTCTGGTAAGAATGCGCCTAAAACAGGCGGTGGGGCCAAAGGAAACAATGCCTCCGCTGCCGGGAGTGGTAATACTAAAAACAGTGCCTCAGGGGCTGATATCAACAACTATGCCGGACAGATAAAATCGGCGATTGAAAGTAAGTTTTATGACGCATCGTCCTATGCGGGCAAAACATGTACCTTGCGTATCAAACTTGCTCCTGACGGCCTGTTGTTAAATATACAGTCCGAAGGTGGTGATCCTGCTCTGTGCCAGGCCGCTCTTGCCGCAGCCCGACAGGCTAAGTTTCCGAAACCACCTAGCCAGGCAGTATATGAAGTCTTCAAAAATGCGCCACTGGACTTCAAACCTCAG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,589 total letters Query= IDP96885.107 Length=1635 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2219 0.0 2_11C_C8_IDP96885.107_T7term.seq 1712 0.0 1_08C_C8_IDP96885.107_T7S.seq 1308 0.0 F V * L * E G D I H M H H H H H H S S G V D L W S H P Q F Query_1 74 ATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCG 163 1 24 ..........CT...--...................N................ 74 E K G T E N L Y F Q S N A S S F D E H L D A S A G G G G G S Query_1 164 AAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCAGTTCGTTTGACGAGCATCTGGATGCCTCTGCCGGCGGCGGCGGCGGATCGT 253 0 1 .................................................... 52 1 75 ............................................................................... 153 S I D A V M V D P G A V V N N Y N R Q Q Q Q Q A S A R R A A Query_1 254 CGATTGATGCCGTCATGGTCGATCCGGGGGCGGTGGTAAATAACTATAACCGTCAGCAACAGCAGCAGGCCAGCGCACGTCGCGCCGCTG 343 0 53 .......................................................................................... 142 1 154 .......................................................................................... 243 E Q R E K Q A Q Q Q A E E L R E K Q A A E Q E R L K Q L E Q Query_1 344 AACAGCGTGAAAAACAGGCGCAGCAGCAGGCGGAAGAGTTACGTGAGAAACAGGCGGCGGAACAGGAACGGCTGAAACAGCTCGAACAGG 433 0 143 .......................................................................................... 232 0 422 ............ 433 0 500 ............ 511 2 867 ............ 856 2 946 ........... 936 1 244 .........................N.TN.......................N.............N....................... 333 E R L Q A Q E A A K E A K E Q Q K Q A E E A A Query_1 434 AGCGGCTGCAG--GCGCAG---GAAGCGGCGAAAGAAGC---GAAGGA-GCA-GCAG--AAG-CAG--GC------TGAAGAA-GCGGCT 501 0 233 ...........--......---.................---......-...-....--...-...--..------.......-...... 300 0 517 .............CT..---.GCC..-.GC-....--...-A.A--..------G....CG-...... 567 0 744 ...........CA.A.A..GCT.C...T.AT....CG..---C..--.-..G-....--C..-..------------A...CC-.....G 810 0 583 ...---.......A....A....---CC..C.-.G.A..G.CT...-...--..GGCAGC....A..-...... 645 0 685 ..C... 690 0 826 ..T...........---. 840 0 606 ....---........ 617 2 782 ...---.......A....A....CCA.C....A..G-..TA--...-...--..GGCAGC....A..-...... 719 2 759 ....---........T....C..----.CG.C.-..T-.A.A--...-.G.CT..------C...A..-..C... 704 2 850 .............CT..---.GCC..-.GC-....--...AA.A--..------G....CG-...... 799 2 620 ...........CA.A.A..GCT.C...T.AT....CG..---C..--.-..G-....--C..-..------------A...CC-.....G 554 2 679 ..C... 674 2 538 ..T...........---. 524 1 334 ...........--......---..N..............---......-...-....--...-...--..------.......-...... 401 1 733 ..------....A..A...... 748 A K A A A A A K A K A D A Q A K E A Q E A A A Query_1 502 GCCAAG---GC-------CGCCGCGGCGGCAAAAGCCAAAGCGGACGCACAGGCAAAAGAAG-CGC--AGGAAGCCGCTGC-C---AA-- 572 0 301 ......---..-------............................................-...--.............-.---..-- 371 0 568 ..G...AAA..GCAACA-G.AA....A.AA.......C.GCA...A..---...T..-------..--...CG..A....A-A---..-- 638 0 811 ..G...---AA-------A..G.....T..G....---......... 844 0 646 ...G.A---AA-------A.....T.A.---......---..C.-....-----.....CG.C...TG..A.G.....C..-.GAG..-- 710 0 691 .AG...---..-------...... 704 0 778 ..G..-.---..-- 785 0 320 ..-------............ 307 0 802 ...........G..---G.......CG..---T..G.......-.. 840 0 812 ..-------......... 802 2 1001 ...-.---..-- 996 2 718 ...G.A---AA-------A.....T.A.---......NCC...C.---.A.A..GGCC.CT.-A.A--...CC.....C.A-G---..-- 654 2 703 .AG..A---..-------.N....----....-----.......C...------------T.-A.A--...CC.....C.A-G---..-- 654 2 798 ..G...AAA..GCAAACAG.AA....A.AA.......C.GCA...A..---...T...------..--...CG..A....A-A---..-- 726 2 553 ..G...---AA-------A..G.....T..G....---......... 520 2 673 .AG...---..-------...... 660 2 586 ..G..-.---..-- 579 2 562 ...........G..---G.......CG..---T..G.......-.. 524 2 552 ..-------......... 562 1 402 ......---..-------....N..N.........T..N.N....N.....NN.........-.N.--.............-.---..-- 472 1 567 ...........-AGCG..-- 583 1 768 .......C..G.---..NA 783 1 749 ... 751 K A A A E A K A K A D A Q A K A A E Q A A A K A A A D A Query_1 573 AGCGGCCGCCGAGGCGAAAGCGAAGGCGGATGCCCAGGCG---AAAGCG---GCAGAACAGGCGGCGGCCAAGGCGGCTGCTGACGC-GA 655 0 372 ........................................---......---...................................-.. 454 0 639 ......T.....---A.....CGCT.A.A.A...GCC...CAA......GCC..T..GA....C..C...G..AAA..C..A.C...T.. 725 0 711 ...C..A....CT.AA.....---.....-----..-...---......---..------T..A..A.AG.....T..A...------.. 773 0 786 ......A.. 794 0 191 ............ 202 2 995 .N...................................-..---......---...............A...................-.. 914 2 653 ...C..A....CT.AA.....---.....-----..-...---......---..------T..A..A.AG.....T..A...------.. 591 2 653 ...C..A....CT.AA.....---.....-----..-...---......---..------T..A..A.AG.....T..A...------.. 591 2 725 ......T.....---A.....CGCT.A.A.A...NCC...CAA......GCC..T..GA....C..C...G..AAA..C..A.C...T.. 639 2 578 ......A.. 570 1 473 ..N........................N............---.....N---.....N................T............-.. 555 1 584 ...C..T........... 601 1 784 .........T.. 795 K K Q A E A A A A K A A A E A K K Q A E A E A A K A A Query_1 656 AAAAGCAG-----GCCGAAGCCGCTGCAGC-GA-AAGCCGCTGCCGAGGCGAA-GAAACAGGCGGAAGCTGAAGCGGCGAAAGCTGCGGC 737 0 455 ........-----.................-..-...................-.................................... 536 0 250 .............AA...AA 269 0 726 ......G.CGGCA..GA....G........A..G...--.....---A..TG.-T...GC...CA....G.C...A..A.....C..... 809 0 774 T.....-.-----...A....G..A.....-A.-.......G..---......-....GC..... 826 0 188 .-........... 199 2 913 ........-----.................-..-...................A.................................... 831 2 590 T.....-.-----...A....G..A.....-A.-.......G..---......-....GC..... 538 2 590 T.....-.-----...A....G..A.....-A.-.......G..---......-....GC..... 538 2 638 ......G.CGGCA..GA....G........A..G...--.....---A..TG.-T...GC...CA....G.C...A..A.....C..... 555 1 556 ....N...-----.................-..-...................-......T......N.........N.........N.. 637 1 351 ..N..........AA...AA 370 1 459 ...........C----.-...NG..C...........-AGCGA....N..T..CC.---.........N..A.A 523 A E A Q K K A E A A A A K K A Q Q E A E K K A Q Q E A A Query_1 738 CGAGGCGCAGAAGAAA-GCGGAAGCGGCGGCTGCGAAG-AAAGCGCAA-CAGGAAGCGGAGAAAAAAGCCCAGCAGGAAGC---GGCTAA 821 0 537 ................-.....................-.........-................................---...... 620 0 270 G...CA.......C.G-..T....AA........C...----..-------C.CC....C.GC.......A.AGC...C..ACA...A.. 347 0 810 ---....A...----------................A-G..... 840 0 247 ..........C....G....GA..G..C.-..---A.--.. 281 2 830 ................A.....................-.........A................................---...... 745 2 554 ---....A...----------................A-G..... 524 1 638 .........N..T...-..N...N...N.........NA........N-....N..................N..T.....---...... 722 1 371 G...CA.......C.G-..T....AA........C... 407 1 524 NC..... 530 1 347 ......N..---...G.. 361 K Q A A A E K A A A E K A A E K A A A Q K A Query_1 822 ------GCAGGCG--GCAGCTG-AAAAAGCGGCTgccga---aaaagccgctgag---------aaagccgccgcgca---aaaagcggc 887 0 621 ------.......--.......-................---.............---------..............---......... 686 0 348 AGAAGC.....AA--..C....-CC........C.....GGCG.....GAAG.C.---------G.T...CAG...------......-- 417 0 282 ------......--------..-..G...........A.---GGCC.....G.C.GCAAAAGCC.....G.A...A..GGC.....AA.. 353 2 744 A-----.......--.......-................---.............---------......N.......---......... 678 1 723 ------T......--N......A................---............T---------..............---.N....... 789 1 362 ------AG.A...AA.G...A.-C.G....A.--..T..---.G....G....CC---------..G......N.. 416 A A E K A A A E K A A A A E K A A A A K A A Query_1 888 cgctgagaaggccgccg-ccgagaaagccgcagccgctgaaaaagC-G---GCGG-----CAG-C-G---AAAGCGGC------T----- 951 0 687 .................-............................-.---....-----...-.-.---........------.----- 750 0 418 -..A..AC....G..G.-..A..GCG..T..T.A...-........-A---.----------.-.-C---G....C..------.----- 474 0 233 ......------.----- 239 0 354 -..A.-....-....T.-..---.....G..C....AG.CG.....-.AAG....ATGCC...G.-.---........AGAACAG----- 426 2 677 .................-............................-.---....-----...-.-.---........------.----- 614 2 746 .....A.---....-----...-.-TGAA........------.GCCGA 714 2 901 ....-C---..T.-----...-.-.---.....C..------.----- 879 2 961 ...-.-.---........------A----- 948 1 790 ......T..T.....NNTN....T.....N..N........N....-.---N...-----..A-.TN---......NN------.----- 855 1 568 ....-C---..T.-----...-.-.---.....C..------.----- 590 1 740 ........------.----- 748 1 334 ......------.----- 340 A A E K A A A D K A A K A A A A Query_1 952 ----GCA---GCAGAG---A-------AG--GCTGC---AGCT-----GATaaagc-gg-cc------aa--agcggc-agcagca---- 999 0 751 ----...---......---.-------..--.....---....-----........-..-..------..--......-.......---- 798 0 475 ----...---..-..----.-------..CC.....CGAG..G-----A.G...CA-..-.G------G.--...T.A-...G..G---- 525 0 240 ----...---.--.C.---C-------..-----.A---...G-----.CG....A-A.-.G------..GG...A..-..A....GGCT 288 0 427 ----..G---..G.CC---.-------..--..G..---T...GACGC..A.....A..-..------G.--...C..-T.....G---- 480 2 613 ----...---......---.-------..--.....---....-----........-..-..------..--......-.......---- 566 2 713 AAAA..C---..T...---.-------.A--..CN.---C..G-----C.A.....-..-..GCTGAG..--G..C..-C..C.AG---- 656 2 878 ----..C---.AG.C.---.AAGAAAC..--..G.A---....-----.---....-..-.G------..--...T..-G..C.------ 829 2 947 ----.A.CAG..G.C.ACC.-------..--..G..---T...GACGC..A.....A..-..------G.--...C..-T.....G---- 888 1 856 ----...---N..N..---N-------..--.....---NN..-----.......N-..T.A------..--......A...NTT.---- 905 1 591 ----.. 592 1 749 ----..C---.A.A.------------------A..---C...-----........-C.-.. 775 1 341 ----...---. 344 K A A A A K K A A A A K E A D G V D N L L G Query_1 1000 aaag-ccg-cggc---ggcg-aa--ga---------aagcggc-ggctgcgaaag---aagcggacggcgTTG-ACAACCTGCT-CGGCG 1066 0 799 ....-...-....---....-..--..---------.......-...........---...............-..........-..... 865 0 526 ....-.T.-....CGA....-C.--..AGAAAGCGG.......-.........GA---..... 579 0 289 G...-AA.-....---T..C-..--.G---------CC..C..-...G..A....CCA......... 337 0 481 ....-...-.T..CGA....-..--..---------..CA...-.. 511 2 565 ....-...-....---....-..--..---------.......-...........---...............-..........-..... 499 2 655 ....-...-.A..---C..T-G.--A.---------.......-...A....... 619 2 828 --..-G..-.A.A---A.AA-..GC.G---------.......-.........GA---..... 787 2 887 ....-...-.T..CGA....A..--..---------..CA...-.. 856 1 906 N...TN..C....---.N..A..--T.---------N..NNNNT.TT...AN...---.....N..N.T....A.....TG...C..N.N 978 D L S S G K N A P K T G G G A K G N N A S A A G S G N T Query_1 1067 -ATCTGAGTTCTGGTAAGAATGCGCCTAAAACAGGCGGTGGGGCCAAAGGAAACAA-TGCC--TCCGCTG--CCGGGAGTGGTAATACTA 1150 0 866 -.......................................................-....--.......--.................. 949 2 498 -.......................................................-....--.......--.................. 415 1 979 TN...N...........A.....NN..T.....N.T.NN....N.....A...T..T..N.CT...NN.NGC......N....NN..N.N 1068 K N S A S G A D I N N Y A G Q I K S A I E S K F Y D A S S Query_1 1151 AAAACAGTGCCTCAGGGG--CTGATATCAACAACTATGCCGGACAGATAAAATCGGCGATTG-AAAGTAAGTTTTATGACGCATCGTCC- 1236 0 950 ..................--..........................................-..........................- 1035 2 414 ..................--..........................................-..........................- 329 1 1069 ....NNN.N.NN.C....GT....N....C.....N.T.....A.A..T.N...C..NN...N.......T....TGN..N...NC.N.T 1158 Y A G K T C T L R I K L A P D G L L L N I Q S E G G D P A L Query_1 1237 TATGCGGGCAAAACATGTACCTTGCGTATCAAACTTGCTCCTGACGGCCTGTTGTTAAATATACAGTCCGAAGGTGGTGATCCTGCTCTG 1326 0 1036 .......................................................................................... 1125 2 328 .......................................................................................... 239 1 1159 ...NG..CN....AN.A.C.T...G..T.N..CT..T..N....N........ 1211 C Q A A L A A A R Q A K F P K P P S Q A V Y E V F K N A P L Query_1 1327 TGCCAGGCCGCTCTTGCCGCAGCCCGACAGGCTAAGTTTCCGAAACCACCTAGCCAGGCAGTATATGAAGTCTTCAAAAATGCGCCACTG 1416 0 1126 .......................................................................................... 1215 2 238 .......................................................................................... 149 D F K P Q * H W K W I T D P N S S S V D K L A A A L E H H H Query_1 1417 GACTTCAAACCTCAGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccac 1506 0 1216 ............... 1230 2 148 .......................................................................................... 59 H H H * D P A A N K A R Query_1 1507 caccaccacTGAGATCCGGC-TGCTAACAAAGCCCGAAA 1544 2 58 ....................T.................. 20 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5732879 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 3,589 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96887.106 (IDP96887.1-538.F..pMCSG53)
MLAPRCRRVTMCNKTLTLNRTAQQPVELPADYDRLLQSITVLAPVQKISLDIDVTHKAALPAVLESALLLVAQLEIVSPSELGGFFGLADHERQVLVDEMIDTGLVRYNDEGDIATTVKLTMQRREGASDEGIAIEDVENHRDFTFIDLCTGHIQPKCNADLQKGLPELPRKINRSDFTDLIASQFRRFQICLPDTKSKRALRSSKARLYRVNRASVGQSGLKQQISLDIHVHSDPLNGIRLDSRLMDYSLEHTRLMESSGLKTEAINWLHERKLEAASSTLEDYCDLAKDMVIRRYIGRDGKLELGRLLHDRQRRKTSYGNSTMQRMMIGPVYAAPNRNTLIKWAERLPRQQQVHQGIWLGATNELFGASLGLGSFIKQMNTELASGERNSRLNLAFQCDEEFRAQRLITNQFNSRTDGSLRIFPKGRSESHLEILVFPGEYGMAVVQYHAALDPSLGYAGLTLPIGYFTTDPEQVAQLWMQVQQRIQSPVRSADQPEVATDDLNKLLGCAPAELAQMLVDNSEEKLKKLVERFNQH
ATGCTTGCGCCGAGGTGCAGGAGAGTGACTATGTGTAACAAGACCCTTACCTTGAACAGAACAGCGCAGCAACCTGTGGAACTGCCGGCTGATTATGACCGCTTGCTACAAAGTATTACCGTGCTGGCACCAGTACAGAAGATCAGTCTGGACATTGATGTGACGCATAAAGCAGCGCTACCAGCGGTTCTTGAGTCAGCTCTTCTACTAGTGGCGCAACTCGAAATCGTGTCTCCTTCTGAGTTAGGAGGCTTTTTCGGACTCGCTGACCATGAGCGTCAGGTTCTGGTTGATGAAATGATCGACACTGGCCTCGTGCGATACAATGATGAAGGCGATATCGCGACTACAGTCAAGCTGACAATGCAGCGCCGAGAAGGCGCATCGGATGAGGGCATTGCAATTGAGGACGTGGAAAATCACCGTGACTTCACCTTCATCGACCTTTGCACTGGGCACATTCAGCCTAAGTGTAATGCGGACCTGCAGAAAGGTTTGCCCGAGTTGCCACGTAAGATCAATCGCAGTGACTTCACCGATTTGATCGCTAGCCAATTTCGTCGCTTTCAGATTTGCTTACCCGATACCAAGAGCAAGCGCGCACTGCGTAGCTCGAAGGCCCGACTCTACCGAGTGAATCGGGCCTCTGTTGGTCAATCGGGGTTGAAACAGCAGATCTCACTCGATATCCACGTCCATTCCGATCCTCTGAACGGGATACGTCTGGACTCACGGTTGATGGACTATAGCTTAGAGCATACACGCTTGATGGAAAGCTCCGGTCTCAAAACAGAGGCTATTAACTGGCTGCACGAGCGCAAGTTAGAGGCCGCTAGTTCCACACTAGAAGACTATTGTGATCTGGCAAAAGATATGGTGATCCGCCGATATATCGGACGTGATGGTAAGCTGGAACTAGGGCGTTTACTCCATGATCGCCAGCGCAGAAAAACCAGCTATGGTAACAGCACGATGCAACGTATGATGATTGGGCCTGTCTATGCAGCCCCCAATCGTAACACTTTAATTAAGTGGGCTGAACGGTTGCCACGTCAGCAGCAGGTACATCAAGGAATCTGGCTTGGTGCTACCAACGAGTTATTTGGCGCTAGTTTAGGTCTCGGGTCGTTCATCAAACAAATGAACACCGAGCTCGCAAGTGGAGAGCGCAACAGTCGCCTCAATTTGGCATTTCAGTGCGATGAGGAATTTCGGGCACAGCGTTTAATCACCAATCAGTTTAATAGCCGTACCGACGGTAGCCTACGAATTTTCCCAAAAGGCCGTTCTGAGTCGCACTTGGAAATATTGGTTTTCCCTGGCGAGTACGGTATGGCAGTGGTTCAGTACCATGCTGCTTTGGATCCGTCTCTTGGGTATGCTGGCCTTACATTGCCAATCGGCTACTTCACGACGGACCCTGAGCAGGTAGCTCAGTTGTGGATGCAGGTGCAGCAACGAATCCAGTCACCGGTTCGGTCAGCCGACCAGCCCGAGGTAGCGACAGATGATCTTAATAAGCTACTGGGTTGTGCTCCTGCAGAATTGGCACAAATGCTAGTCGACAATTCCGAGGAAAAACTGAAAAAACTGGTGGAAAGATTCAATCAACAC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,145 total letters Query= IDP96887.106 Length=2019 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2911 0.0 2_12D_D9_IDP96887.106_T7term.seq 1912 0.0 1_09D_D9_IDP96887.106_T7S.seq 1692 0.0 P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 60 CCCCTCT-AGAAATAATTTT-GTTTAACTTT-AAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTG 146 1 16 ...T...N............T..........N......................................-------------------- 85 W S H P Q F E K G T E N L Y F Q S N A M L A P R C R R V T M Query_1 147 GTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGCTTGCGCCGAGGTGCAGGAGAGTGACTATGTG 236 0 1 ................................... 35 1 86 ----------------------------.....................T........................................ 147 C N K T L T L N R T A Q Q P V E L P A D Y D R L L Q S I T V Query_1 237 TAACAAGACCCTTACCTTGAACAGAACAGCGCAGCAACCTGTGGAACTGCCGGCTGATTATGACCGCTTGCTACAAAGTATTACCGTGCT 326 0 36 .......................................................................................... 125 1 148 .......................................................................................... 237 L A P V Q K I S L D I D V T H K A A L P A V L E S A L L L V Query_1 327 GGCACCAGTACAGAAGATCAGTCTGGACATTGATGTGACGCATAAAGCAGCGCTACCAGCGGTTCTTGAGTCAGCTCTTCTACTAGTGGC 416 0 126 .......................................................................................... 215 1 238 .......................................................................................... 327 A Q L E I V S P S E L G G F F G L A D H E R Q V L V D E M I Query_1 417 GCAACTCGAAATCGTGTCTCCTTCTGAGTTAGGAGGCTTTTTCGGACTCGCTGACCATGAGCGTCAGGTTCTGGTTGATGAAATGATCGA 506 0 216 .......................................................................................... 305 1 328 .......................................................................................... 417 D T G L V R Y N D E G D I A T T V K L T M Q R R E G A S D E Query_1 507 CACTGGCCTCGTGCGATACAATGATGAAGGCGATATCGCGACTACAGTCAAGCTGACAATGCAGCGCCGAGAAGGCGCATCGGATGAGGG 596 0 306 .......................................................................................... 395 1 418 .......................................................................................... 507 G I A I E D V E N H R D F T F I D L C T G H I Q P K C N A D Query_1 597 CATTGCAATTGAGGACGTGGAAAATCACCGTGACTTCACCTTCATCGACCTTTGCACTGGGCACATTCAGCCTAAGTGTAATGCGGACCT 686 0 396 .......................................................................................... 485 0 520 ....G.A........... 537 1 508 .......................................................................................... 597 1 632 ....G.A........... 649 L Q K G L P E L P R K I N R S D F T D L I A S Q F R R F Q I Query_1 687 GCAGAAAGGTTTGCCCGAGTTGCCACGTAAGATCAATCGCAGTGACTTCACCGATTTGATCGCTAGCCAATTTCGT-CGC-TTTCAGATT 774 0 486 ............................................................................-...-......... 573 0 418 ....A.C........... 435 2 1199 .....N..A...-N.CA..... 1179 1 598 ............................................................................-...T......... 686 1 530 ....A.C........... 547 C L P D T K S K R A L R S S K A R L Y R V N R A S V G Query_1 775 TGCTTA-CCCGAT-ACCAAGAGCAAGCGCGCACTGCG--TAGCTCGAAGGCCCG-ACTCTACCGAGTG-AAT-CGGGCCT-CTGTTGG-T 855 0 574 ......-......-.......................--...............-.............-...-.......-.......-. 654 2 1178 G....NN......N....N..NN....NN...N...NGNN...CN...NN....N...NNN.......-...T.......T..N....N. 1090 1 687 ......-......-.......................--...............-.............A...-.......-.......-. 768 Q S G L K Q Q I S L D I H V H S D P L N G I R L D S R L Query_1 856 CA-ATCGGGGTTGAAAC-AGC-AGA-TCTCACTCGATATCCACGTCCATTCCGATCCTCTGAACGGGATACGTCTGGACTCACGGTTGAT 941 0 655 ..-..............-...-...-................................................................ 740 2 1089 ..N......A.-.....N...N...A....N.........-..N........................................-..... 1003 1 769 ..-..............-...-...-................................................................ 854 M D Y S L E H T R L M E S S G L K T E A I N W L H E R K L E Query_1 942 GGACTATAGCTTAGAGCATACACGCTTGATGGAAAGCTCCGGTCTCAAAACAGAGGCTA-TTAAC-TGGCTGCACGAGCGCAAGTTAGAG 1029 0 741 ...........................................................-.....-........................ 828 2 1002 .........N.................................................-.....-........................ 915 1 855 ........................................................N..T.....T.......N.......-........ 943 A A S S T L E D Y C D L A K D M V I R R Y I G R D G K L Query_1 1030 GCCGCTAGTTCCACACTAGAAGACTA-TTGTGATCTGGCAAAAGATATGGTGATCCG-CCGAT-ATAT-CGGACGTGATGGTAAGCTGGA 1115 0 829 ..........................-..............................-.....-....-..................... 914 0 896 .....-....-... 885 2 914 ..N.......................-..............................-.....-....-..................... 829 2 847 .....-....-... 858 1 944 ...........N..............N...............GAT........N...C.....A....C......N.............. 1033 E L G R L L H D R Q R R K T S Y G N S T M Q R M M I G P Query_1 1116 A-CTAGGGCGTTTACT-CCATGATCGCCAGCGCAGAAAAACCAGCTATGGTAACAGCA-CGATGCAAC--GTATGATGATT-GGG--CCT 1197 0 915 .-..............-.........................................-.........--...........-...--... 996 2 828 .-..............-.........................................-.........--...........T...--... 746 1 1034 .N..............C..................-......CN........N.NN..C...N..C..CGC........A.-...GC.T. 1121 V Y A A P N R N T L I K W A E R L P R Q Q Q V H Q G I W Query_1 1198 GTC--TATGCAGCCCCCAATCGTAA-CACTTTAATTAAGT--GGGCTGAA-CGGTTGCCACGTCAGCAGCAGGTACATCAAGGAATCTGG 1281 0 997 ...--....................-..............--........-....................................... 1080 2 745 ...--....................-..............--........-....................................... 662 1 1122 ...CTNN....NN........NNN.C.N....NN......NN.NN.....C...N.... 1180 L G A T N E L F G A S L G L G S F I K Q M N T E L A S G E R Query_1 1282 CTTGGTGCTACCAACGAGTTATTTGGCGCTAGTTTAGGTCTCGGGTCGTTCATCAAACAAATGAACACCGAGCTCGCAAGTGGAGAGCGC 1371 0 1081 .......................................................................................... 1170 2 661 .......................................................................................... 572 N S R L N L A F Q C D E E F R A Q R L I T N Q F N S R T D G Query_1 1372 AACAGTCGCCTCAATTTGGCATTTCAGTGCGATGAGGAATTTCGGGCACAGCGTTTAATCACCAATCAGTTTAATAGCCGTACCGACGGT 1461 0 1171 .......................................................................................... 1260 2 571 .......................................................................................... 482 S L R I F P K G R S E S H L E I L V F P G E Y G M A V V Q Y Query_1 1462 AGCCTACGAATTTTCCCAAAAGGCCGTTCTGAGTCGCACTTGGAAATATTGGTTTTCCCTGGCGAGTACGGTATGGCAGTGGTTCAGTAC 1551 0 1261 .......................................................................................... 1350 2 481 .......................................................................................... 392 H A A L D P S L G Y A G L T L P I G Y F T T D P E Q V A Q L Query_1 1552 CATGCTGCTTTGGATCCGTCTCTTGGGTATGCTGGCCTTACATTGCCAATCGGCTACTTCACGACGGACCCTGAGCAGGTAGCTCAGTTG 1641 0 1351 .......................................................................................... 1440 2 391 .......................................................................................... 302 W M Q V Q Q R I Q S P V R S A D Q P E V A T D D L N K L L G Query_1 1642 TGGATGCAGGTGCAGCAACGAATCCAGTCACCGGTTCGGTCAGCCGACCAGCCCGAGGTAGCGACAGATGATCTTAATAAGCTACTGGGT 1731 0 1441 .......................................................................................... 1530 2 301 .......................................................................................... 212 C A P A E L A Q M L V D N S E E K L K K L V E R F N Q H * H Query_1 1732 TGTGCTCCTGCAGAATTGGCACAAATGCTAGTCGACAATTCCGAGGAAAAACTGAAAAAACTGGTGGAAAGATTCAATCAACACTAACAT 1821 0 1531 .................................................................................... 1614 2 211 .......................................................................................... 122 W K W I T D P N S S S V D K L A A A L E H H H H H H * D P A Query_1 1822 TGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCT 1911 2 121 .......................................................................................... 32 A N K A R K E Query_1 1912 GCTAACAAAGCCCGAAAGGAAG 1933 2 31 ..................A... 10 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 8196188 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 4,145 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96894.104 (IDP96894.1-248.F..pMCSG53)
MLISTYQERQTRNREKIKQLLNFLKEETYSDFKTLMLLFGFRDHKSLYSLLAKTERMGLIQKHVLESRTMKISLWGITSDGLAVVLTPDDKIFPARFEPSKITGWTLEHHLDNQAARLILEKKGASGWINGDRATFISQYQVKHRPDGLLTLPDGKVIAIETERRLKTRARYQSIMASHLLARTRKQWIYVFYVVPDQQKKRALELLFDSIRHVIINHQRVPLEERHRLVFRIYTVDELKHLSLDRYT
ATGCTGATTTCGACATATCAAGAGCGCCAGACACGCAACAGAGAAAAAATAAAACAACTGCTGAACTTCCTGAAAGAAGAAACCTACAGCGATTTTAAAACGCTGATGCTGCTATTTGGTTTCCGGGATCATAAGTCGCTTTATTCGCTACTGGCAAAAACAGAGCGAATGGGGTTAATACAGAAGCATGTGCTTGAATCACGAACAATGAAGATTTCATTATGGGGGATCACCAGCGACGGGCTGGCGGTGGTGTTAACCCCGGATGATAAAATCTTTCCTGCTCGGTTTGAACCATCAAAAATCACCGGCTGGACGCTGGAACACCATCTTGATAATCAGGCCGCCAGGCTTATTCTGGAGAAGAAAGGCGCTTCAGGATGGATTAACGGTGACCGTGCCACCTTTATCAGCCAGTATCAGGTGAAACATCGCCCGGACGGGTTACTGACTCTGCCTGACGGTAAGGTTATTGCCATTGAAACCGAACGCCGCCTGAAAACCCGAGCCCGTTATCAGTCGATCATGGCAAGCCACCTGCTGGCCCGTACCCGCAAGCAGTGGATTTACGTTTTTTATGTTGTGCCCGATCAACAGAAGAAGCGTGCGCTTGAACTGCTGTTTGACAGCATCAGGCATGTCATCATCAACCATCAGCGCGTCCCGCTGGAAGAACGCCACCGTCTGGTGTTTCGCATTTATACCGTTGATGAACTGAAACACCTGTCATTAGACCGCTATACG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,237 total letters Query= IDP96894.104 Length=1149 Score E Sequences producing significant alignments: (Bits) Value 1_08D_D8_IDP96894.104_T7S.seq 1734 0.0 2_11D_D8_IDP96894.104_T7term.seq 1728 0.0 0_wt 1342 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAA 90 1 6 ..T.............-................... 40 2 1031 .......................................................................................... 942 2 964 ...G...........T.T.C.C.C...........A... 1002 E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E N Query_1 91 GAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAAC 180 1 41 ....................................------------------------------------------------...... 82 2 941 ....................................------------------------------------------------...... 900 L Y F Q S N A M L I S T Y Q E R Q T R N R E K I K Q L L N F Query_1 181 CTGTACTTCCAATCCAATGCCATGCTGATTTCGACATATCAAGAGCGCCAGACACGCAACAGAGAAAAAATAAAACAACTGCTGAACTTC 270 1 83 .......................................................................................... 172 2 899 .......................................................................................... 810 0 1 ..................................................................... 69 L K E E T Y S D F K T L M L L F G F R D H K S L Y S L L A K Query_1 271 CTGAAAGAAGAAACCTACAGCGATTTTAAAACGCTGATGCTGCTATTTGGTTTCCGGGATCATAAGTCGCTTTATTCGCTACTGGCAAAA 360 1 173 .......................................................................................... 262 2 809 .......................................................................................... 720 0 70 .......................................................................................... 159 T E R M G L I Q K H V L E S R T M K I S L W G I T S D G L A Query_1 361 ACAGAGCGAATGGGGTTAATACAGAAGCATGTGCTTGAATCACGAACAATGAAGATTTCATTATGGGGGATCACCAGCGACGGGCTGGCG 450 1 263 .......................................................................................... 352 2 719 .......................................................................................... 630 0 160 .......................................................................................... 249 V V L T P D D K I F P A R F E P S K I T G W T L E H H L D N Query_1 451 GTGGTGTTAACCCCGGATGATAAAATCTTTCCTGCTCGGTTTGAACCATCAAAAATCACCGGCTGGACGCTGGAACACCATCTTGATAAT 540 1 353 .......................................................................................... 442 2 629 .......................................................................................... 540 0 250 .......................................................................................... 339 Q A A R L I L E K K G A S G W I N G D R A T F I S Q Y Q V K Query_1 541 CAGGCCGCCAGGCTTATTCTGGAGAAGAAAGGCGCTTCAGGATGGATTAACGGTGACCGTGCCACCTTTATCAGCCAGTATCAGGTGAAA 630 1 443 .......................................................................................... 532 2 539 .......................................................................................... 450 0 340 .......................................................................................... 429 H R P D G L L T L P D G K V I A I E T E R R L K T R A R Y Q Query_1 631 CATCGCCCGGACGGGTTACTGACTCTGCCTGACGGTAAGGTTATTGCCATTGAAACCGAACGCCGCCTGAAAACCCGAGCCCGTTATCAG 720 1 533 .......................................................................................... 622 2 449 .......................................................................................... 360 0 430 .......................................................................................... 519 S I M A S H L L A R T R K Q W I Y V F Y V V P D Q Q K K R A Query_1 721 TCGATCATGGCAAGCCACCTGCTGGCCCGTACCCGCAAGCAGTGGATTTACGTTTTTTATGTTGTGCCCGATCAACAGAAGAAGCGTGCG 810 1 623 .......................................................................................... 712 2 359 .......................................................................................... 270 0 520 .......................................................................................... 609 L E L L F D S I R H V I I N H Q R V P L E E R H R L V F R I Query_1 811 CTTGAACTGCTGTTTGACAGCATCAGGCATGTCATCATCAACCATCAGCGCGTCCCGCTGGAAGAACGCCACCGTCTGGTGTTTCGCATT 900 1 713 .......................................................................................... 802 2 269 .......................................................................................... 180 0 610 .......................................................................................... 699 Y T V D E L K H L S L D R Y T * H W K W I T D P N S S S V D Query_1 901 TATACCGTTGATGAACTGAAACACCTGTCATTAGACCGCTATACGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGAC 990 1 803 .......................................................................................... 892 2 179 .......................................................................................... 90 0 700 ............................................. 744 K L A A A L E H H H H H H * D P A A N K A R K E A E L A A Query_1 991 AAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAG-ATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGC 1079 1 893 -...............................N..........-..............-............................... 979 2 89 ...........................................A.........................A.... 16 A T A E Q * L A * P L G A S K R V L R G F L L K Query_1 1080 CACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAA 1149 1 980 ...........-.......................NN............-...-................ 1046 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3613137 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 3,237 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96903.104 (IDP96903.1-141.F..pMCSG53)
MRQRTIVCPLIENEGHYLLCKMAADRGVFPGQWALSGGGVEPGERIEEALRREIREELGEKLILTHIAPWCFRDDTRVKTYPDGHQETIYMIYLIFNCVSANRDVTINEEFDDYAWVKAEDLKNYDLNAATRVTLSLKGLL
ATGCGCCAAAGGACGATTGTTTGCCCGCTGATCGAGAATGAAGGCCATTATCTGCTTTGCAAAATGGCCGCCGACCGCGGGGTTTTTCCCGGGCAATGGGCCTTATCCGGCGGCGGCGTGGAGCCAGGGGAACGTATTGAAGAGGCGCTGCGGCGGGAAATACGCGAAGAGCTCGGCGAAAAACTCATCCTTACCCATATCGCGCCATGGTGCTTTCGCGACGATACCCGCGTCAAAACCTACCCCGACGGGCATCAGGAAACGATCTACATGATCTATCTCATCTTCAACTGCGTGAGCGCCAATCGCGATGTGACGATCAACGAGGAGTTTGACGATTACGCCTGGGTCAAAGCCGAAGATCTCAAAAACTACGATCTTAATGCCGCCACCCGCGTTACTCTCAGCCTGAAAGGGCTACTT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,858 total letters Query= IDP96903.104 Length=828 Score E Sequences producing significant alignments: (Bits) Value 1_08E_E8_IDP96903.104_T7S.seq 1200 0.0 2_11E_E8_IDP96903.104_T7term.seq 1155 0.0 0_wt 764 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAA 90 1 13 ..........-................... 41 2 705 .......................................................................................... 616 2 638 ...G...........T.T.C.C.C...........A... 676 E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E N Query_1 91 GAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAAC 180 1 42 ....................................------------------------------------------------...... 83 2 615 ....................................------------------------------------------------...... 574 L Y F Q S N A M R Q R T I V C P L I E N E G H Y L L C K M A Query_1 181 CTGTACTTCCAATCCAATGCCATGCGCCAAAGGACGATTGTTTGCCCGCTGATCGAGAATGAAGGCCATTATCTGCTTTGCAAAATGGCC 270 1 84 .......................................................................................... 173 2 573 .......................................................................................... 484 0 1 ..................................................................... 69 A D R G V F P G Q W A L S G G G V E P G E R I E E A L R R E Query_1 271 GCCGACCGCGGGGTTTTTCCCGGGCAATGGGCCTTATCCGGCGGCGGCGTGGAGCCAGGGGAACGTATTGAAGAGGCGCTGCGGCGGGAA 360 1 174 .......................................................................................... 263 2 483 .......................................................................................... 394 0 70 .......................................................................................... 159 I R E E L G E K L I L T H I A P W C F R D D T R V K T Y P D Query_1 361 ATACGCGAAGAGCTCGGCGAAAAACTCATCCTTACCCATATCGCGCCATGGTGCTTTCGCGACGATACCCGCGTCAAAACCTACCCCGAC 450 1 264 .......................................................................................... 353 2 393 .......................................................................................... 304 0 160 .......................................................................................... 249 G H Q E T I Y M I Y L I F N C V S A N R D V T I N E E F D D Query_1 451 GGGCATCAGGAAACGATCTACATGATCTATCTCATCTTCAACTGCGTGAGCGCCAATCGCGATGTGACGATCAACGAGGAGTTTGACGAT 540 1 354 .......................................................................................... 443 2 303 .......................................................................................... 214 0 250 .......................................................................................... 339 Y A W V K A E D L K N Y D L N A A T R V T L S L K G L L * H Query_1 541 TACGCCTGGGTCAAAGCCGAAGATCTCAAAAACTACGATCTTAATGCCGCCACCCGCGTTACTCTCAGCCTGAAAGGGCTACTTTAACAT 630 1 444 .......................................................................................... 533 2 213 .......................................................................................... 124 0 340 .................................................................................... 423 W K W I T D P N S S S V D K L A A A L E H H H H H H * D P A Query_1 631 TGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCT 720 1 534 .......................................................................................... 623 2 123 .......................................................................................... 34 A N K A R K E A E L A A A T A E Q * L A * P L G A S K R V L Query_1 721 GCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTG 810 1 624 .......................................................................................... 713 2 33 .................-..... 12 R G F L L K Query_1 811 AGGGGTTTTTTGCTGAAA 828 1 714 .................. 731 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 2286969 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 2,858 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96910.105 (IDP96910.1-253.F..pMCSG53)
MDDLLDAITALSSLISSEKIRAIAENISHIDDNKSMATLYNVVNTPIAVTIVKQLTMAWQKTSVSSSELAAMLLTASHVYSKATSEQSTELVWTGPTTPFVSARRTEQALLQVISTAERTLFITSFVAYDVSTIVKALNAANERGVMISILLELSQEKGGSLNFDTIEKMRIQVPSAKLYVWNNKDNEYYGGCVHAKVAVADARECFITSANLTGYAMEKNIEAGVLISGGSIPRQLNEHLFSLIKTKIICLV
ATGGATGATCTTCTGGACGCTATTACAGCGCTGAGCTCTCTAATATCATCTGAAAAAATAAGAGCTATCGCTGAAAATATTAGCCATATTGATGATAATAAATCTATGGCCACATTATACAATGTGGTCAATACTCCAATAGCTGTAACAATAGTCAAACAACTCACTATGGCCTGGCAAAAAACGTCTGTCAGTTCTAGTGAGTTGGCTGCAATGCTCCTTACTGCCAGCCATGTCTATTCAAAAGCCACTTCAGAGCAATCTACCGAGTTAGTGTGGACGGGCCCCACAACACCATTTGTTTCAGCACGTCGTACTGAACAGGCTTTGCTTCAAGTTATTAGTACCGCTGAGCGCACTCTGTTTATAACCAGCTTCGTCGCTTATGATGTTTCAACGATAGTGAAAGCATTAAATGCTGCAAATGAACGCGGAGTTATGATCTCAATTTTACTTGAATTATCACAAGAGAAAGGTGGTAGTTTAAATTTTGATACCATTGAGAAGATGAGAATACAGGTCCCTTCAGCCAAACTTTATGTATGGAATAATAAAGATAATGAATATTATGGCGGATGTGTTCATGCAAAAGTTGCTGTTGCTGATGCTCGGGAGTGCTTTATCACTAGTGCAAACCTTACTGGGTATGCTATGGAAAAAAATATTGAAGCTGGGGTGCTTATTTCTGGCGGAAGTATTCCAAGGCAACTTAATGAGCATCTTTTTTCCCTTATTAAAACTAAAATTATCTGCTTGGTT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 2,838 total letters Query= IDP96910.105 Length=1164 Score E Sequences producing significant alignments: (Bits) Value 1_08F_F8_IDP96910.105_T7S.seq 1786 0.0 2_11F_F8_IDP96910.105_T7term.seq 1451 0.0 0_wt 1370 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 58 TTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGG 147 1 9 .....T.......-.......................................................--------------------- 76 S H P Q F E K G T E N L Y F Q S N A M D D L L D A I T A L S Query_1 148 TCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGGATGATCTTCTGGACGCTATTACAGCGCTGAGC 237 1 77 ---------------------------............................................................... 139 0 1 .................................... 36 S L I S S E K I R A I A E N I S H I D D N K S M A T L Y N V Query_1 238 TCTCTAATATCATCTGAAAAAATAAGAGCTATCGCTGAAAATATTAGCCATATTGATGATAATAAATCTATGGCCACATTATACAATGTG 327 1 140 .......................................................................................... 229 2 849 ....N....N..........................-............N...............GA................... 765 0 37 .......................................................................................... 126 V N T P I A V T I V K Q L T M A W Q K T S V S S S E L A A Query_1 328 GTCAA-TACTCCAATAGCTGTAACAATAGTCAAACAACTCACTATGGCCTGGCAAAAAACGTCTGTCAGTTCTAGTGAGTTGGCTGCAAT 416 1 230 .....-.................................................................................... 318 1 982 ............ 993 2 764 .....A...................................-.N...........................N.................. 676 0 127 .....-.................................................................................... 215 M L L T A S H V Y S K A T S E Q S T E L V W T G P T T P F V Query_1 417 GCTCCTTACTGCCAGCCATGTCTATTCAAAAGCCACTTCAGAGCAATCTACCGAGTTAGTGTGGACGGGCCCCACAACACCATTTGTTTC 506 1 319 .......................................................................................... 408 2 675 .......................................................................................... 586 0 216 .......................................................................................... 305 S A R R T E Q A L L Q V I S T A E R T L F I T S F V A Y D V Query_1 507 AGCACGTCGTACTGAACAGGCTTTGCTTCAAGTTATTAGTACCGCTGAGCGCACTCTGTTTATAACCAGCTTCGTCGCTTATGATGTTTC 596 1 409 .......................................................................................... 498 2 585 .......................................................................................... 496 0 306 .......................................................................................... 395 S T I V K A L N A A N E R G V M I S I L L E L S Q E K G G S Query_1 597 AACGATAGTGAAAGCATTAAATGCTGCAAATGAACGCGGAGTTATGATCTCAATTTTACTTGAATTATCACAAGAGAAAGGTGGTAGTTT 686 1 499 .......................................................................................... 588 2 495 .........................................................N................................ 406 0 396 .......................................................................................... 485 L N F D T I E K M R I Q V P S A K L Y V W N N K D N E Y Y G Query_1 687 AAATTTTGATACCATTGAGAAGATGAGAATACAGGTCCCTTCAGCCAAACTTTATGTATGGAATAATAAAGATAATGAATATTATGGCGG 776 1 589 .......................................................................................... 678 2 405 .......................................................................................... 316 0 486 .......................................................................................... 575 G C V H A K V A V A D A R E C F I T S A N L T G Y A M E K N Query_1 777 ATGTGTTCATGCAAAAGTTGCTGTTGCTGATGCTCGGGAGTGCTTTATCACTAGTGCAAACCTTACTGGGTATGCTATGGaaaaaaaTAT 866 1 679 .......................................................................................... 768 2 315 .......................................................................................... 226 0 576 .......................................................................................... 665 I E A G V L I S G G S I P R Q L N E H L F S L I K T K I I C Query_1 867 TGAAGCTGGGGTGCTTATTTCTGGCGGAAGTATTCCAAGGCAACTTAATGAGCATCTTTTTTCCCTTATTAAAACTAAAATTATCTGCTT 956 1 769 .......................................................................................... 858 2 225 .......................................................................................... 136 0 666 .......................................................................................... 755 L V * H W K W I T D P N S S S V D K L A A A L E H H H H H H Query_1 957 GGTTTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTG 1046 1 859 .......................................................................................... 948 2 135 .......................................................................................... 46 0 756 .... 759 * D P A A N K A R K E A E L A A A T A E Q * L A * P L G A S Query_1 1047 AGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAA 1136 1 949 ...................................................................................N...... 1038 1 304 ............ 315 2 45 ...........................A... 15 2 690 ............ 679 0 201 ............ 212 K R V L R G F L L K Query_1 1137 ACGGGTCTTGAGGGGTTTTTTGCTGAAA 1164 1 1039 .......-.......-............ 1064 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3202920 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 2,838 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96916.105 (IDP96916.1-906.F..pMCSG53)
MTRGQYPMSSVKSLLFSHNHHLLSVKGCEAGLDVLAFEGDEALSQPFRYRIEFTSADHAISKEMMLMKAASLTLQAPVAQGFGINVQQPVRVIQGVVTGFERLSTSRDETHYALTLQPRLALLNRSHQNAIYQDQSVPQIVEKILRERHGLRGQDFLFSLTKTYPRREQVMQYGEDDLRFITRLLGEVGIWFRFTADTRLHIDVAEFCDSQQGYEKGLTLPSVPPSGQQSAGVDAVWEMACRHRVVEQQVSTRDYNYREATADMNAQVDVTRGETTTFGEAYHWGDNYLTAGNVHDRHPAPESGAFYARLRHERYLNGQTRMQATTSCPTLCPGQVLKVTGGEEVAGEFADGVLVTAMHSHARRDADFAVEFAGIPDSPDVGYRPEPGARPVMAGTLPARVTSTRENDTYGHIDKHGRYRVNMLFDRARWETGFESLWVRQSRPYAGDTYGLHLPLLAGTEVAIGFEDGNPDRPYIAGVLHDSAHGDHVTIRNDKRNVLRTPANNKIRLDDERGKEHIKVSTEYGGKSQLNLGHLVDAEKQPRGEGFELRTDSWGAIRAQKGIFISADGQVQAQGQVLDMEPAVSNLAEAREQMMSISGDAQKATANPADLQAQITLLEQQLTDLKKSVLLVSAPEGIALTSGEHLQVSAGHNLIATAGKNADVSVVKNLFIGVGSALSVFVRKLGIRLIANQGPVQMQAQNDLMALLARKEISIVSTEDEIKIIAKKKLTLNGGGSYIRLDANAIESATAGEYRTKAGYYGRQESASEKVKMPVFPVMEPGEHSLRFAFPGADSVSQSMNWLINQPYSISDSAGKILSTGTVDTSGRFPKIVLPESDTLNLIIGRETWSKEELSVSSTEDDSMEDFEDEAPDADLHLSEMGFEDDRTSALTESMISTLLSLTKDS
TTGACGAGGGGACAATATCCGATGAGTAGCGTGAAATCGTTGTTGTTCAGCCATAACCACCACCTGCTTTCGGTGAAGGGATGTGAAGCCGGGCTTGATGTGCTGGCCTTCGAGGGCGATGAAGCCCTGAGCCAGCCGTTCCGCTACCGTATTGAATTCACCAGCGCAGACCATGCCATCAGCAAAGAGATGATGCTGATGAAGGCAGCCTCCCTGACGCTGCAGGCGCCGGTGGCTCAGGGCTTCGGCATCAATGTTCAGCAACCCGTGCGGGTGATCCAGGGCGTGGTGACGGGGTTTGAACGGCTCAGTACCTCCAGGGATGAAACGCACTACGCGCTGACGCTTCAGCCGCGCCTGGCGCTGCTTAACCGCTCGCACCAGAACGCCATTTACCAGGACCAGTCCGTTCCGCAGATTGTGGAAAAAATCCTCCGCGAGCGCCACGGCCTGCGCGGTCAGGATTTTCTGTTCTCGCTGACAAAAACGTATCCGCGACGCGAGCAGGTGATGCAGTACGGCGAGGATGACCTGCGCTTTATCACCCGGCTGCTGGGTGAGGTGGGGATCTGGTTTCGGTTTACCGCCGACACGCGGCTGCACATCGACGTCGCTGAGTTCTGCGACAGTCAGCAGGGCTATGAGAAGGGGCTGACGTTGCCTTCGGTCCCGCCGTCAGGGCAGCAGTCGGCCGGGGTGGACGCGGTGTGGGAGATGGCATGTCGCCACCGCGTCGTGGAGCAGCAGGTCAGTACCCGCGACTACAACTACCGCGAGGCGACGGCGGACATGAACGCGCAGGTGGATGTCACCCGCGGGGAGACCACTACCTTTGGTGAGGCTTACCACTGGGGAGATAACTACCTGACCGCGGGGAATGTCCATGACCGCCATCCGGCGCCGGAGTCCGGGGCGTTTTACGCCCGGCTTCGGCATGAGCGTTATCTTAACGGCCAGACCCGGATGCAGGCCACCACCAGCTGCCCGACGCTCTGCCCGGGACAGGTGCTGAAGGTCACCGGCGGAGAGGAGGTGGCCGGGGAGTTTGCTGATGGCGTGCTCGTCACGGCGATGCACAGCCACGCCCGGCGCGATGCCGACTTTGCGGTGGAGTTTGCCGGCATACCGGACAGCCCGGACGTCGGCTACCGGCCTGAGCCCGGCGCGCGTCCGGTGATGGCCGGGACGCTGCCGGCCCGCGTGACCAGCACCCGGGAAAACGACACCTACGGGCATATCGACAAACACGGACGCTACCGGGTGAATATGCTGTTTGACCGGGCGCGCTGGGAAACCGGTTTCGAGAGCCTGTGGGTCCGCCAGTCGCGGCCGTATGCCGGAGACACCTACGGCCTGCACCTGCCGCTGCTGGCCGGGACGGAGGTGGCGATTGGCTTTGAGGATGGTAACCCTGACCGGCCGTATATCGCGGGCGTGCTGCACGACTCGGCGCACGGCGACCACGTCACCATCCGCAATGACAAACGTAACGTCCTGCGCACCCCGGCCAACAACAAAATCCGCCTCGACGATGAGCGCGGAAAAGAGCATATCAAGGTCTCGACGGAGTACGGCGGTAAGAGCCAGCTGAACCTCGGCCATCTCGTGGATGCTGAAAAGCAGCCGCGCGGCGAAGGCTTTGAGCTGAGAACCGACAGCTGGGGCGCTATCCGGGCACAGAAAGGGATATTTATCAGCGCGGACGGACAGGTGCAGGCTCAGGGGCAGGTGCTGGATATGGAGCCGGCGGTCAGCAATCTGGCCGAAGCCCGGGAGCAGATGATGTCCATCTCCGGTGACGCGCAGAAGGCGACCGCGAACCCGGCCGATCTCCAGGCGCAAATTACCCTGCTGGAGCAGCAGCTGACAGACCTCAAAAAATCGGTACTGCTGGTCAGCGCGCCGGAGGGGATAGCGCTGACCAGCGGGGAGCACCTGCAGGTCTCAGCCGGGCATAACCTGATTGCCACAGCCGGAAAGAATGCAGACGTCAGCGTGGTGAAAAATCTGTTCATCGGGGTGGGCAGTGCGCTGAGCGTGTTTGTGCGAAAGCTTGGGATCAGGCTGATAGCGAATCAGGGACCGGTACAGATGCAGGCGCAGAATGACCTGATGGCGCTGCTTGCCCGGAAGGAAATCAGCATTGTCAGCACGGAGGATGAAATCAAAATCATCGCGAAGAAGAAGCTGACCCTGAATGGTGGGGGGAGTTATATCAGACTTGATGCAAATGCGATTGAATCTGCTACTGCAGGCGAATATCGCACGAAAGCAGGATATTACGGGCGTCAGGAAAGCGCTTCTGAAAAAGTCAAAATGCCTGTATTTCCTGTAATGGAGCCTGGAGAGCACAGTTTACGTTTTGCTTTTCCAGGTGCGGATAGTGTCAGCCAGAGTATGAACTGGCTGATAAATCAGCCGTACAGTATTTCTGATAGCGCTGGAAAAATTCTGTCTACCGGAACAGTAGACACTTCAGGTCGTTTTCCCAAAATAGTACTTCCTGAAAGCGATACATTAAATTTAATCATTGGCCGAGAAACTTGGAGTAAAGAGGAGCTTTCTGTCTCATCAACTGAAGACGACAGTATGGAGGATTTTGAGGATGAAGCACCTGATGCCGATCTTCATTTAAGTGAAATGGGTTTTGAAGATGATAGAACATCAGCATTGACTGAATCAATGATTTCAACTTTATTAAGTTTAACAAAGGATAGT
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 5,207 total letters Query= IDP96916.105 Length=3123 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4902 0.0 2_11G_G8_IDP96916.105_T7term.seq 1930 0.0 1_08G_G8_IDP96916.105_T7S.seq 1761 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L W Query_1 58 TTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGG 147 1 11 .............-.................N....................................A--------------------- 78 S H P Q F E K G T E N L Y F Q S N A L T R G Q Y P M S S V K Query_1 148 TCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCTTGACGAGGGGACAATATCCGATGAGTAGCGTGAAA 237 0 1 .................................... 36 1 79 ---------------------------..N........................A................................... 141 S L L F S H N H H L L S V K G C E A G L D V L A F E G D E A Query_1 238 TCGTTGTTGTTCAGCCATAACCACCACCTGCTTTCGGTGAAGGGATGTGAAGCCGGGCTTGATGTGCTGGCCTTCGAGGGCGATGAAGCC 327 0 37 .......................................................................................... 126 0 246 ...... 241 1 142 .......................................................................................... 231 1 351 ...... 346 L S Q P F R Y R I E F T S A D H A I S K E M M L M K A A S L Query_1 328 CTGAGCCAGCCGTTCCGCTACCGTATTGAATTCACCAGCGCAGACCATGCCATCAGCAAAGAGATGATGCTGATGAAGGCAGCCTCCCTG 417 0 127 .......................................................................................... 216 0 1062 ..G..C............ 1045 0 240 ........ 233 1 232 .......................................................................................... 321 1 345 ........ 338 T L Q A P V A Q G F G I N V Q Q P V R V I Q G V V T G F E R Query_1 418 ACGCTGCAGGCGCCGGTGGCTCAGGGCTTCGGCATCAATGTTCAGCAACCCGTGCGGGTGATCCAGGGCGTGGTGACGGGGTTTGAACGG 507 0 217 .......................................................................................... 306 0 134 .............. 121 0 926 ........... 936 1 322 .......................................................................................... 411 1 239 .............. 226 1 1128 ............. 1140 L S T S R D E T H Y A L T L Q P R L A L L N R S H Q N A I Y Query_1 508 CTCAGTACCTCCAGGGATGAAACGCACTACGCGCTGACGCTTCAGCCGCGCCTGGCGCTGCTTAACCGCTCGCACCAGAACGCCATTTAC 597 0 307 .......................................................................................... 396 0 2114 ........... 2124 2 733 ........... 723 1 412 .......................................................................................... 501 Q D Q S V P Q I V E K I L R E R H G L R G Q D F L F S L T K Query_1 598 CAGGACCAGTCCGTTCCGCAGATTGTGGAAAAAATCCTCCGCGAGCGCCACGGCCTGCGCGGTCAGGATTTTCTGTTCTCGCTGACAAAA 687 0 397 .......................................................................................... 486 1 502 .......................................................................................... 591 T Y P R R E Q V M Q Y G E D D L R F I T R L L G E V G I W F Query_1 688 ACGTATCCGCGACGCGAGCAGGTGATGCAGTACGGCGAGGATGACCTGCGCTTTATCACCCGGCTGCTGGGTGAGGTGGGGATCTGGTTT 777 0 487 .......................................................................................... 576 1 592 .......................................................................................... 681 R F T A D T R L H I D V A E F C D S Q Q G Y E K G L T L P Query_1 778 CGGTTTACCG-CCGACACGCGGCTGCACATCGACGTCGCTGAGTTCTGCGACAGTCAGCAGGGCTATGAGAAGGGGCTGACG-TTGCCTT 865 0 577 ..........-.......................................................................-....... 664 1 682 ..........N.......................................................................T....... 771 S V P P S G Q Q S A G V D A V W E M A C R H R V V E Q Q V S Query_1 866 CGGTCCCGCCGTCAGGGCAGCAGTCGGCCGGGGTGGACGCGGTGTGGGAGATGGCATGTCGCCACCGCGTCGTGGAGCAGCAGGTCAGTA 955 0 665 .......................................................................................... 754 0 1853 ........... 1863 2 999 ...NC........... 984 1 772 .............N...........................................................-................ 860 T R D Y N Y R E A T A D M N A Q V D V T R G E T T T F G E A Query_1 956 CCCGCGACTACAACTACCGCGAGGCGACGGCGGACATGAACGCGCAGGTGGATGTCACCCGCGGGGAGACCACTACCTTTGGTGAGGCTT 1045 0 755 .......................................................................................... 844 1 861 .......................................................................................... 950 Y H W G D N Y L T A G N V H D R H P A P E S G A F Y A R L R Query_1 1046 ACCACTGGGGAGATAACTACCTGACCGCGGGGAATGTCCATGACCGCCATCCGGCGCC-GGAGTCCGGGGCGTTTTACGCCCGGCTTCGG 1134 0 845 ..........................................................-............................... 933 0 905 .........-... 894 0 241 ........ 248 1 951 ......-..............................................T....N................N.............. 1039 1 346 ........ 353 H E R Y L N G Q T R M Q A T T S C P T L C P G Q V L K V Query_1 1135 CATGAGCGTTATCTTAACGGCCAGACCCGGA-TGCAGGCCACCA-CCAGCT-GCCCGACGC-TCTGCCC--GGGACAGGTGCTGAAGGTC 1218 0 934 ...............................-............-......-.........-.......--................... 1017 0 249 ... 251 1 1040 .N...N.......-..N..............NNN..........C......N....N.NN.NNN.....TG..A............N.N. 1128 1 354 ... 356 T G G E E V A G E F A D G V L V T A M H S H A R R D A D Query_1 1219 A--CCG-GCGG--AGAGGAGGTGGCCGGGGAGTTTGCTGATGGCGTGCTCGTCACGGCGATGCACAGCCACGCCCGGCGCGATGCCGACT 1303 0 1018 .--...-....--............................................................................. 1102 0 186 ............A..G.. 169 1 1129 .AC...T....GA.A....NN.NN.....NGN..N.... 1167 1 291 ............A..G.. 274 F A V E F A G I P D S P D V G Y R P E P G A R P V M A G T L Query_1 1304 TTGCGGTGGAGTTTGCCGGCATACCGGACAGCCCGGACGTCGGCTACCGGCCTGAGCCCGGCGCGCGTCCGGTGATGGCCGGGACGCTGC 1393 0 1103 .......................................................................................... 1192 0 1363 ...C..C........... 1380 P A R V T S T R E N D T Y G H I D K H G R Y R V N M L F D R Query_1 1394 CGGCCCGCGTGACCAGCACCCGGGAAAACGACACCTACGGGCATATCGACAAACACGGACGCTACCGGGTGAATATGCTGTTTGACCGGG 1483 0 1193 .......................................................................................... 1282 0 1342 ........... 1352 2 357 ........... 367 A R W E T G F E S L W V R Q S R P Y A G D T Y G L H L P L L Query_1 1484 CGCGCTGGGAAACCGGTTTCGAGAGCCTGTGGGTCCGCCAGTCGCGGCCGTATGCCGGAGACACCTACGGCCTGCACCTGCCGCTGCTGG 1573 0 1283 .......................................................................................... 1372 0 1171 ...G..A... 1180 0 1222 ........... 1232 A G T E V A I G F E D G N P D R P Y I A G V L H D S A H G D Query_1 1574 CCGGGACGGAGGTGGCGATTGGCTTTGAGGATGGTAACCCTGACCGGCCGTATATCGCGGGCGTGCTGCACGACTCGGCGCACGGCGACC 1663 0 1373 .......................................................................................... 1462 0 1181 ........ 1188 H V T I R N D K R N V L R T P A N N K I R L D D E R G K E H Query_1 1664 ACGTCACCATCCGCAATGACAAACGTAACGTCCTGCGCACCCCGGCCAACAACAAAATCCGCCTCGACGATGAGCGCGGAAAAGAGCATA 1753 0 1463 .......................................................................................... 1552 I K V S T E Y G G K S Q L N L G H L V D A E K Q P R G E G F Query_1 1754 TCAAGGTCTCGACGGAGTACGGCGGTAAGAGCCAGCTGAACCTCGGCCATCTCGTGGATGCTGAAAAGCAGCCGCGCGGCGAAGGCTTTG 1843 0 1553 .......................................................................................... 1642 E L R T D S W G A I R A Q K G I F I S A D G Q V Q A Q G Q Query_1 1844 AGCTGAGAACCGACAGCTGGGGCGCTATCCGGGCACAGAAAGGGA-TATTTAT-CAGCGCGGACGGA-CAGGTGC-AGGCTCAGGGGCAG 1929 0 1643 .............................................-.......-.............-.......-.............. 1728 0 1362 .......-.... 1352 2 1196 ....N...CGN.N..T.C...NN..G..N...A.....T.T.......-.....N.N.....C......NA...... 1121 V L D M E P A V S N L A E A R E Q M M S I S G D A Q K A T Query_1 1930 GTGCTGGATATGGAGCCGGCGGTCAGCAATCTGGCCGAA-GCCCGGGAG-CAGATGATG-TCCATCTCCGGTGACGCGCAGAAGGCGACC 2016 0 1729 .......................................-.........-.........-.............................. 1815 2 1120 ..N..............NNN...................A.........N.........N.....N.......N-.N............. 1032 A N P A D L Q A Q I T L L E Q Q L T D L K K S V L L V S A Query_1 2017 -GCGAACCCGGCCGATCTCCAGGCGCAAATTACCCTGCTGGAGCAGCAGCTGACAGACCTCAAAAAATCGGTACTGCTGGTCAGCGCGCC 2105 0 1816 -......................................................................................... 1904 0 1926 ............ 1915 0 737 ........... 747 2 1031 N...........................N....G-.N...............N..................................... 943 2 921 ............ 932 P E G I A L T S G E H L Q V S A G H N L I A T A G K N A D V Query_1 2106 GGAGGGGATAGCGCTGACCAGCGGGGAGCACCTGCAGGTCTCAGCCGGGCATAACCTGATTGCCACAGCCGGAAAGAATGCAGACGTCAG 2195 0 1905 .......................................................................................... 1994 0 1901 ............ 1890 2 942 .......................................N...........NN........N............................ 853 2 946 ............ 957 S V V K N L F I G V G S A L S V F V R K L G I R L I A N Q G Query_1 2196 CGTGGTGAAAAATCTGTTCATCGGGGTGGGCAGTGCGCTGAGCGTGTTTGTGCGAAAGCTTGGGATCAGGCTGATAGCGAATCAGGGACC 2285 0 1995 .......................................................................................... 2084 2 852 .......................................................................................... 763 P V Q M Q A Q N D L M A L L A R K E I S I V S T E D E I K I Query_1 2286 GGTACAGATGCAGGCGCAGAATGACCTGATGGCGCTGCTTGCCCGGAAGGAAATCAGCATTGTCAGCACGGAGGATGAAATCAAAATCAT 2375 0 2085 .......................................................................................... 2174 2 762 .......................................................................................... 673 1 464 ........... 474 I A K K K L T L N G G G S Y I R L D A N A I E S A T A G E Y Query_1 2376 CGCGAAGAAGAAGCTGACCCTGAATGGTGGGGGGAGTTATATCAGACTTGATGCAAATGCGATTGAATCTGCTACTGCAGGCGAATATCG 2465 0 2175 .......................................................................................... 2264 2 672 .......................................................................................... 583 R T K A G Y Y G R Q E S A S E K V K M P V F P V M E P G E H Query_1 2466 CACGAAAGCAGGATATTACGGGCGTCAGGAAAGCGCTTCTGAAAAAGTCAAAATGCCTGTATTTCCTGTAATGGAGCCTGGAGAGCACAG 2555 0 2265 .......................................................................................... 2354 2 582 .......................................................................................... 493 S L R F A F P G A D S V S Q S M N W L I N Q P Y S I S D S A Query_1 2556 TTTACGTTTTGCTTTTCCAGGTGCGGATAGTGTCAGCCAGAGTATGAACTGGCTGATAAATCAGCCGTACAGTATTTCTGATAGCGCTGG 2645 0 2355 .......................................................................................... 2444 2 492 .......................................................................................... 403 G K I L S T G T V D T S G R F P K I V L P E S D T L N L I I Query_1 2646 AAAAATTCTGTCTACCGGAACAGTAGACACTTCAGGTCGTTTTCCCAAAATAGTACTTCCTGAAAGCGATACATTAAATTTAATCATTGG 2735 0 2445 .......................................................................................... 2534 0 2528 ............ 2517 0 1224 ........... 1214 2 402 .......................................................................................... 313 G R E T W S K E E L S V S S T E D D S M E D F E D E A P D A Query_1 2736 CCGAGAAACTTGGAGTAAAGAGGAGCTTTCTGTCTCATCAACTGAAGACGACAGTATGGAGGATTTTGAGGATGAAGCACCTGATGCCGA 2825 0 2535 .......................................................................................... 2624 2 312 .......................................................................................... 223 D L H L S E M G F E D D R T S A L T E S M I S T L L S L T K Query_1 2826 TCTTCATTTAAGTGAAATGGGTTTTGAAGATGATAGAACATCAGCATTGACTGAATCAATGATTTCAACTTTATTAAGTTTAACAAAGGA 2915 0 2625 .......................................................................................... 2714 2 222 .......................................................................................... 133 D S * H W K W I T D P N S S S V D K L A A A L E H H H H H H Query_1 2916 TAGTTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTG 3005 0 2715 .... 2718 2 132 .......................................................................................... 43 * D P A A N K A R K E Query_1 3006 AGATCCGGCTGCT-AACAAAGCCCGAAAGGAAG 3037 2 42 .............A..........N........ 10 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 16000065 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 5,207 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96925.104 (IDP96925.1-239.F..pMCSG53)
MKMIVTEDYEEMSLVASHHVLGYITAPRRVNLAVTAGSTPKRMYEHLTAAVKGKAFYDRVHYYNFDEIPFRGQSREGVTISNLRQLFFTPAQIKEENIHKLTLDNAAQHDRQLEEAGGLDLMVLGLGADGHFCGNLPNTTRFHDQTVEVPIHGEMIALIANSEMGGDISAVPNSYVTMGPRSVMAAKNLLLIVSGAAKAHALKQVVEGPVSVQVPASVLKLHPSLVIIADKAAAAELQQ
ATGAAAATGATTGTGACCGAAGATTATGAAGAGATGAGCCTGGTCGCCAGCCATCATGTTCTGGGCTATATCACGGCGCCGCGGCGGGTGAATCTGGCGGTGACGGCGGGGAGCACGCCAAAGCGGATGTATGAGCATTTGACCGCCGCCGTGAAAGGCAAAGCATTCTATGATCGGGTGCACTATTACAACTTCGATGAGATCCCTTTTCGCGGCCAGTCGCGAGAAGGCGTCACGATATCTAATTTGCGCCAGCTGTTTTTTACCCCGGCGCAGATCAAAGAAGAGAACATTCACAAGCTGACGCTGGACAACGCCGCTCAGCACGATCGCCAGCTGGAAGAGGCTGGCGGTCTGGATCTGATGGTGCTGGGGCTGGGGGCCGACGGCCATTTTTGCGGCAACCTGCCGAATACCACCCGCTTTCATGATCAGACGGTCGAAGTGCCGATTCATGGGGAAATGATTGCTCTTATCGCTAACAGCGAGATGGGCGGGGATATCTCGGCAGTACCAAACAGCTATGTCACCATGGGGCCGAGAAGCGTGATGGCGGCGAAAAATTTATTGCTAATTGTCAGCGGTGCGGCAAAAGCGCACGCGTTAAAGCAAGTGGTGGAAGGTCCGGTCAGCGTGCAGGTGCCGGCTTCAGTGCTCAAATTGCACCCTTCACTGGTGATTATTGCCGATAAAGCGGCGGCGGCGGAGTTGCAGCAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,195 total letters Query= IDP96925.104 Length=1122 Score E Sequences producing significant alignments: (Bits) Value 1_08H_H8_IDP96925.104_T7S.seq 1687 0.0 2_11H_H8_IDP96925.104_T7term.seq 1653 0.0 0_wt 1294 0.0 S R S R E I N T T H Y R G I V S G * Q F P S R N N F V * L * Query_1 1 TCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAA 90 1 17 ..T.............................. 49 2 996 .............................................................................-.........-.. 909 2 929 ...G...........T.T.C.C.C...........A... 967 E G D I H M H H H H H H S S G V D L W S H P Q F E K G T E N Query_1 91 GAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAAC 180 1 50 ..............................---------------------------------------------------..A.....N 88 2 908 ....................................------------------------------------------------...... 867 L Y F Q S N A M K M I V T E D Y E E M S L V A S H H V L G Y Query_1 181 CTGTACTTCCAATCCAATGCCATGAAAATGATTGTGACCGAAGATTATGAAGAGATGAGCCTGGTCGCCAGCCATCATGTTCTGGGCTAT 270 1 89 ..........................................N............................................... 178 2 866 .......................................................................................... 777 0 1 ..................................................................... 69 I T A P R R V N L A V T A G S T P K R M Y E H L T A A V K G Query_1 271 ATCACGGCGCCGCGGCGGGTGAATCTGGCGGTGACGGCGGGGAGCACGCCAAAGCGGATGTATGAGCATTTGACCGCCGCCGTGAAAGGC 360 1 179 .......................................................................................... 268 2 776 .......................................................................................... 687 0 70 .......................................................................................... 159 K A F Y D R V H Y Y N F D E I P F R G Q S R E G V T I S N L Query_1 361 AAAGCATTCTATGATCGGGTGCACTATTACAACTTCGATGAGATCCCTTTTCGCGGCCAGTCGCGAGAAGGCGTCACGATATCTAATTTG 450 1 269 .......................................................................................... 358 2 686 .......................................................................................... 597 0 160 .......................................................................................... 249 R Q L F F T P A Q I K E E N I H K L T L D N A A Q H D R Q L Query_1 451 CGCCAGCTGTTTTTTACCCCGGCGCAGATCAAAGAAGAGAACATTCACAAGCTGACGCTGGACAACGCCGCTCAGCACGATCGCCAGCTG 540 1 359 .......................................................................................... 448 2 596 .......................................................................................... 507 0 250 .......................................................................................... 339 E E A G G L D L M V L G L G A D G H F C G N L P N T T R F H Query_1 541 GAAGAGGCTGGCGGTCTGGATCTGATGGTGCTGGGGCTGGGGGCCGACGGCCATTTTTGCGGCAACCTGCCGAATACCACCCGCTTTCAT 630 1 449 .......................................................................................... 538 2 506 .......................................................................................... 417 0 340 .......................................................................................... 429 D Q T V E V P I H G E M I A L I A N S E M G G D I S A V P N Query_1 631 GATCAGACGGTCGAAGTGCCGATTCATGGGGAAATGATTGCTCTTATCGCTAACAGCGAGATGGGCGGGGATATCTCGGCAGTACCAAAC 720 1 539 .......................................................................................... 628 2 416 .......................................................................................... 327 0 430 .......................................................................................... 519 S Y V T M G P R S V M A A K N L L L I V S G A A K A H A L K Query_1 721 AGCTATGTCACCATGGGGCCGAGAAGCGTGATGGCGGCGAAAAATTTATTGCTAATTGTCAGCGGTGCGGCAAAAGCGCACGCGTTAAAG 810 1 629 .......................................................................................... 718 2 326 .......................................................................................... 237 0 520 .......................................................................................... 609 Q V V E G P V S V Q V P A S V L K L H P S L V I I A D K A A Query_1 811 CAAGTGGTGGAAGGTCCGGTCAGCGTGCAGGTGCCGGCTTCAGTGCTCAAATTGCACCCTTCACTGGTGATTATTGCCGATAAAGCGGCG 900 1 719 .......................................................................................... 808 2 236 .......................................................................................... 147 0 610 .......................................................................................... 699 A A E L Q Q * H W K W I T D P N S S S V D K L A A A L E H H Query_1 901 GCGGCGGAGTTGCAGCAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccac 990 1 809 ...................................................G...................................... 898 2 146 ...................................................G...................................... 57 0 700 .................. 717 H H H H * D P A A N K A R K E A E L A A A T A E Q * L A * Query_1 991 caccaccacc-acTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACC 1079 1 899 ..........-...........................................................................-... 986 2 56 ..........A...........................CN..A..... 9 P L G A S K R V L R G F L L K Query_1 1080 CCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAA 1122 1 987 .............-................-............ 1027 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 3480582 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 3,195 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96929.106 (IDP96929.1-396.P(399)..pMCSG53)
MFQNVDAYAGDPILSLMEAFQQDPRADKVNLSIGLYYNEQAIIPQLEAVRQAADRLQSQQQKASLYLPMEGLAPYRRAVQTLQFGDNHPALRAGRIATIQTLGGSGALKVGADFLKRYFPDSAVWVSDPTWENHVAIFAGAGFEVHTYPWFDSATRGVNFPAMLAALQQLPPRSIVLLHPCCHNPTGADLTREQWDRVIEVLIGRELIPFLDIAYQGFGRGLDEDAYAIRAIASAGLTALVSNSFSKIFSLYGERVGGLSVVCDNADIAGRVLGQLKATVRRNYSSPPGFGAQVVSQVLNDPELNALWQEEVEAMRTRISAMRVALVKALQAALPAGDFSYLLTQRGMFSYTGFSADQVDVLRQEHGIYLIASGRVCVAGLNHGNIARVASAFAAV
GTGTTTCAGAACGTTGACGCCTATGCGGGCGATCCTATCCTGTCGCTGATGGAAGCCTTTCAACAGGATCCCCGGGCAGACAAAGTTAACCTCAGCATCGGGCTGTACTATAACGAGCAGGCCATTATTCCCCAGCTGGAGGCGGTCCGTCAGGCCGCTGACCGGCTGCAGAGTCAGCAGCAAAAGGCCAGCCTTTATCTGCCGATGGAGGGGCTGGCGCCCTATCGCCGCGCGGTCCAGACCCTGCAGTTTGGCGACAATCATCCGGCGTTGCGTGCCGGACGCATCGCCACCATCCAGACACTGGGGGGCTCCGGCGCGCTAAAGGTGGGCGCTGATTTCCTCAAGCGCTACTTCCCCGACTCCGCGGTCTGGGTCAGCGATCCAACCTGGGAGAACCACGTTGCCATCTTTGCCGGCGCCGGCTTTGAGGTACATACCTATCCCTGGTTCGACAGCGCCACCCGCGGGGTGAATTTCCCCGCTATGCTCGCCGCCCTCCAGCAGCTGCCGCCGCGCAGCATTGTGCTGCTTCACCCTTGCTGCCATAACCCGACGGGGGCCGATCTCACCCGCGAGCAGTGGGATCGCGTGATTGAGGTGCTGATCGGCAGGGAGCTGATCCCATTCCTTGATATCGCCTACCAGGGCTTCGGCCGCGGACTGGACGAGGATGCCTATGCCATTCGCGCCATCGCCAGCGCCGGGCTGACGGCGCTGGTCAGCAACTCGTTTTCCAAGATATTTTCGCTGTATGGCGAGCGGGTTGGCGGCCTGTCAGTGGTCTGTGACAATGCCGATATCGCCGGGCGCGTGCTGGGCCAGCTGAAAGCCACCGTGCGGCGCAACTACTCCAGCCCGCCGGGGTTTGGCGCCCAGGTGGTGTCGCAGGTGCTCAACGATCCCGAGCTGAATGCCCTGTGGCAGGAAGAAGTGGAGGCCATGCGCACCCGCATCAGCGCCATGCGCGTCGCGCTGGTGAAGGCCCTGCAGGCAGCCCTGCCGGCGGGGGATTTTTCCTATCTGCTGACCCAGCGCGGCATGTTCAGTTACACCGGCTTTAGCGCCGATCAGGTAGACGTTCTGCGTCAAGAGCATGGCATCTATCTGATCGCCAGCGGTCGGGTCTGCGTGGCCGGGCTCAACCACGGCAATATTGCGCGGGTAGCCAGCGCCTTCGCCGCGGTC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,726 total letters Query= IDP96929.106 Length=1593 Score E Sequences producing significant alignments: (Bits) Value 0_wt 2143 0.0 2_08B_B2_IDP96929.106_T7term.seq 1912 0.0 1_02B_B2_IDP96929.106_T7S.seq 1734 0.0 Q F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 55 CAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTG 144 1 15 ..T.............-.......................................................------------------ 85 W S H P Q F E K G T E N L Y F Q S N A V F Q N V D A Y A G D Query_1 145 TGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGTGTTTCAGAACGTTGACGCCTATGCGGGCGAT 234 0 1 ................................. 33 1 86 ------------------------------............................................................ 145 P I L S L M E A F Q Q D P R A D K V N L S I G L Y Y N E Q A Query_1 235 CCTATCCTGTCGCTGATGGAAGCCTTTCAACAGGATCCCCGGGCAGACAAAGTTAACCTCAGCATCGGGCTGTACTATAACGAGCAGGCC 324 0 34 .......................................................................................... 123 1 146 .......................................................................................... 235 I I P Q L E A V R Q A A D R L Q S Q Q Q K A S L Y L P M E Query_1 325 ATTATTCCCCAGCTGGAGGCGGTCCGTCAGGCCGCTGACCGGCTGCAGAGTCAGCAGCAAAAGGCCAGCCTTTATCTGCCGATGGAGGG- 413 0 124 .........................................................................................- 212 2 1185 .....NN...T...NN.A.-...N.......N..N.N.NG..........N.NGN.....................N 1110 1 236 .........................................................................................- 324 G L A P Y R R A V Q T L Q F G D N H P A L R A G R I A T I Query_1 414 GCTGGCGCCCTATCGCCGCGCGGTCCAGACCCTGCAGTTT-GGCGACAAT-CAT-CCGGCGTTGCGTGCCGGACGCATCGCCACCATCCA 500 0 213 ........................................-.........-...-................................... 299 2 1109 ......NN...N.NN...NNN..N.......T........T.........T...T................................N.. 1020 1 325 ........................................-.........-...-................................... 411 Q T L G G S G A L K V G A D F L K R Y F P D S A V W V S D P Query_1 501 GACACTGGGGGGCT-CCGGCGCGCTAAAGGTGGGCGCTGATTTCCTCAA-GCGCTACTTCCCCGACTCCGCGGTCTGGGTCAGCGATCCA 588 0 300 ..............-..................................-........................................ 387 2 1019 ..............T.............A.............-......A............................-........... 932 1 412 ..............-..................................-........................................ 499 T W E N H V A I F A G A G F E V H T Y P W F D S A T R G V N Query_1 589 ACCTGGGAGAACCACGTTGCCATCTTTGCCGGCGCCGGCTTTGAGGTACATACCTATCCCTGGTTCGACAGCGCCACCCGCGGGGTGAAT 678 0 388 .......................................................................................... 477 0 426 ............ 415 2 931 .................-........-..............-................................................ 845 2 895 ............ 906 1 500 .......................................................................................... 589 1 538 ............ 527 F P A M L A A L Q Q L P P R S I V L L H P C C H N P T G A D Query_1 679 TTCCCCGCTATGCTCGCCGCCCTCCAGCAGCTGCCGCCGCGCAGCATTGTGCTGCTTCACCCTTGCTGCCATAACCCGACGGGGGCCGAT 768 0 478 .......................................................................................... 567 2 844 .......................................................................................... 755 1 590 .......................................................................................... 679 L T R E Q W D R V I E V L I G R E L I P F L D I A Y Q G F Query_1 769 CTCACCCGCGAGCAGT-GGGATCGCGTGATTGAGGTGCTGATCGGCAGGGAGCTGATCCCA-TTCCTTGATATCGCCTA-CCAGGGCTTC 855 0 568 ................-............................................-.................-.......... 654 2 754 ................-............................................-.................-.......... 668 1 680 ................G............................................T.................C.......... 769 G R G L D E D A Y A I R A I A S A G L T A L V S N S F S K Query_1 856 GGCCGCGGACTGGACGAGGATGCCTATGCCATTCGCGCCATCGCCAGCGCCGGGCTGAC-GGCGCT-GGTCAGCAACTCGTTTTCCAAGA 943 0 655 ...........................................................-......-....................... 742 2 667 ...........................................................-......-....................... 580 1 770 ...........................................................G......G....................... 859 I F S L Y G E R V G G L S V V C D N A D I A G R V L G Q L K Query_1 944 TATTTTCGCTGTATGGCGAGCGGGTTGGCGGCCTGTCAGTGGTCTGTGACAATGCCGATATCGCCGGGCGCGTGC-TGGGCCAGCTGAAA 1032 0 743 ...........................................................................-.............. 831 2 579 ...........................................................................-.............. 491 1 860 ...................................................T...........N...........T.............. 949 A T V R R N Y S S P P G F G A Q V V S Q V L N D P E L Query_1 1033 -GCCACCGT-GCGGCGCAAC-TACTCCAGCCC-GCCGGGGTTTGGCG-CCCAGG-TGGTG-TCGCAGGTGCTCAACGATCCCGAGC-TGA 1114 0 832 -........-..........-...........-..............-......-.....-.........................-... 913 2 490 -........-..........-...........-..............-......-.....-.........................-... 409 1 950 T........G.......C..T...........G..G...........C......T.....T...........TC.N..........T... 1039 N A L W Q E E V E A M R T R I S A M R V A L V K A L Q A A L Query_1 1115 ATGCCCTGTGGCAGGAAGAAGTGGAGGCCATGCGCACCCGCATCAGCGCCATGCGCGTCGCGCTGGTGAAGGCCC-TGCAGGCAGCCCTG 1203 0 914 ...........................................................................-.............. 1002 2 408 ...........................................................................-.............. 320 1 1040 ...T.........-....-..............N.....N...................CG....TGN.......N.............. 1127 P A G D F S Y L L T Q R G M F S Y T G F S A D Q V D V L Query_1 1204 -CCGGCGGGGGA-TTTTTCCTAT--CTGCTGACCCA-GCGCGGCATGTTCAG-TTACACCGGCTTTAGCGCCGATCAGGTAGACGTTCTG 1287 0 1003 -...........-..........--...........-...............-..................................... 1086 2 319 -...........-..........--...........-...............-..................................... 236 1 1128 NN....N.....T........N.TCT....N.....NNN...CN.N...NNNT......N.N......N... 1199 1 844 ..........-.... 831 R Q E H G I Y L I A S G R V C V A G L N H G N I A R V A S A Query_1 1288 CGTCAAGAGCATGGCATCTATCTGATCGCCAGCGGTCGGGTCTGCGTGGCCGGGCTCAACCACGGCAATATTGCGCGGGTAGCCAGCGCC 1377 0 1087 .......................................................................................... 1176 2 235 .......................................................................................... 146 F A A V * H W K W I T D P N S S S V D K L A A A L E H H H H Query_1 1378 TTCGCCGCGGTCTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccac 1467 0 1177 ............ 1188 2 145 .......................................................................................... 56 H H * D P A A N K A R K E Query_1 1468 caccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAG 1507 2 55 ....................................A... 16 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 5800206 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 3,726 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96932.106 (IDP96932.13-806.P(808)..pMCSG53)
RALVEQQLQYTLCVSLNKATHGDIFNAVALAIRHFQQDHFLLSQKRQREERKKRVYYLSMEFLLGQSLRNNLLNMNLLAEMHQVVNDLGFDLDHLLDEEPDAALGNGGLGRLAACFIDSMATLDIAASGHGIKYEYGLFRQSFQNDQQIEHPDDWHSMHSPWLVEHHAQQILIPLGGYIEHSEDVDGNYNPMWLGWKTIIGIPHDYFVSGYRDTTTNKLRLYSAVASDSFNIHIFNRGDYIKAVSDKIASENISKILYPSDEVLTGKELRLTQEYFLVACTLRDIFRDYAEVNDDITFLPQHVAIQLNDTHPALAVVELMRILVDEYRLPWEQAWEITQNTCAYTNHTLMPEALETWPVTLFEKLLPRHLQIIFEINHRFLEEVARRWPDKNHLLSSLSLIDEHRDKQIRMANLAIVGSHRVNGVAWLHSELVKKQLVPDFYFMTPEKFINQTNGVTPRRWVQQANPGLAAFLHQQLGEGWPTDLPLLSSLESLADDTAVIDNIRAIKFANKTALSQLVAQRYGIALDPLAMFDCQVKRIHEYKRQLLNILHVIALYLDIKETGKTIAPKVHLFAGKAAPGYRMAKLIIQLINTVARKINRDPQVAGQLKVVFLEDYKVSLAEKIIPATDLSEQISTAGTEASGTSNMKFAMNGALTIGTYDGANIEIREAVGIDNFYLFGAVAEEIEESRRLGHHHNFYHQNPAMARVVDTLVSGLFTSDRELFAPLHHMLTEGDHYCHLLDFDSYCQAQRQAMADFELPEQWHRRALLNICRMGEFSSDRTIRGYARDIWGI
CGGGCGTTGGTTGAACAGCAATTACAGTACACATTGTGTGTTAGCTTAAATAAAGCTACGCATGGCGATATTTTTAACGCGGTGGCGCTGGCCATTCGTCATTTTCAACAGGACCATTTTTTGTTGAGCCAGAAGCGGCAACGTGAAGAACGTAAAAAGCGCGTTTACTATCTGTCGATGGAGTTTTTACTGGGACAGTCGCTGCGTAATAACCTTTTGAATATGAATTTACTGGCGGAGATGCACCAGGTGGTGAATGACCTGGGGTTTGATCTTGACCACCTTCTTGACGAAGAGCCCGACGCCGCGCTGGGTAACGGAGGGTTAGGGCGCTTAGCCGCCTGTTTTATCGATTCGATGGCCACCCTTGATATTGCCGCATCCGGGCATGGCATCAAATACGAATATGGTCTTTTTCGCCAATCCTTTCAAAATGACCAGCAGATCGAGCATCCGGATGACTGGCATTCCATGCACTCCCCGTGGCTGGTCGAACATCACGCTCAGCAAATCCTGATCCCGCTGGGAGGCTATATTGAACACAGCGAAGATGTTGATGGCAATTACAATCCAATGTGGCTGGGCTGGAAAACGATTATCGGTATACCCCATGACTATTTCGTTTCCGGTTACCGGGATACGACGACCAACAAGCTGCGGCTTTATAGCGCGGTGGCCTCTGACTCCTTTAATATCCATATTTTCAATCGCGGTGATTATATCAAGGCGGTGAGTGATAAAATCGCGTCGGAGAATATTTCAAAAATACTCTATCCATCCGATGAGGTGCTGACCGGAAAAGAGCTGCGGCTGACGCAAGAGTATTTTCTGGTGGCCTGTACGCTGCGGGATATATTTCGCGACTATGCCGAGGTTAATGACGATATTACTTTCCTGCCGCAGCATGTGGCGATTCAGCTGAATGACACCCATCCGGCATTAGCGGTGGTGGAGCTGATGCGTATCCTCGTCGATGAATACCGGCTGCCCTGGGAGCAGGCGTGGGAGATAACGCAAAACACCTGTGCCTACACGAATCATACGTTGATGCCGGAAGCGCTGGAGACCTGGCCGGTGACGCTGTTTGAAAAACTGCTGCCGCGTCATTTGCAAATTATATTTGAGATCAACCATCGTTTCCTTGAAGAGGTGGCCCGGCGCTGGCCGGATAAAAATCATCTGTTATCTTCGCTGTCTTTAATCGACGAACATCGCGATAAGCAAATCCGTATGGCCAATCTGGCGATTGTCGGTAGCCATCGCGTGAACGGGGTAGCATGGCTGCATTCGGAACTGGTCAAGAAACAACTGGTGCCCGACTTCTATTTTATGACCCCGGAGAAATTCATTAATCAGACCAACGGCGTCACGCCGCGACGCTGGGTGCAGCAGGCTAACCCCGGCCTGGCCGCTTTTTTGCACCAACAGCTGGGGGAAGGGTGGCCAACCGATTTACCCTTGCTGTCCAGTCTGGAAAGCCTGGCCGATGATACTGCTGTTATTGATAATATCCGGGCCATTAAATTTGCCAACAAAACCGCGCTCAGCCAGCTGGTCGCTCAACGCTATGGCATCGCCCTCGATCCGCTGGCGATGTTTGATTGTCAGGTCAAACGTATTCATGAATATAAGCGTCAGTTGCTCAATATTCTTCATGTGATCGCGCTTTATCTGGATATCAAAGAGACAGGGAAAACTATCGCGCCTAAGGTGCATTTGTTTGCGGGTAAAGCGGCGCCGGGCTATCGGATGGCGAAGCTGATTATTCAGTTGATTAATACCGTCGCCCGGAAAATTAACCGCGACCCGCAGGTTGCCGGGCAGCTTAAAGTGGTTTTCCTCGAAGATTATAAAGTCTCGCTGGCGGAAAAAATCATTCCGGCCACAGACCTCTCCGAGCAAATTTCCACCGCCGGGACCGAGGCGTCCGGCACCAGCAATATGAAGTTTGCCATGAACGGCGCGTTAACCATCGGCACCTATGATGGGGCGAATATCGAAATTCGCGAGGCCGTGGGGATCGATAATTTTTATCTGTTTGGCGCCGTGGCGGAAGAGATTGAAGAGAGCCGGCGCCTTGGGCATCACCATAATTTCTATCATCAGAACCCGGCTATGGCGCGTGTTGTCGATACCCTCGTGTCTGGGTTGTTCACCAGCGATCGTGAGCTGTTTGCACCCCTTCACCACATGTTGACTGAGGGCGATCATTACTGTCATTTACTGGATTTCGACAGTTATTGTCAGGCCCAGCGGCAGGCGATGGCCGATTTTGAACTGCCGGAACAATGGCACCGTCGGGCCTTACTCAATATTTGCCGGATGGGCGAATTTTCCAGCGACAGAACCATACGTGGCTACGCCCGGGATATTTGGGGTATC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,518 total letters Query= IDP96932.106 Length=2787 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4296 0.0 1_02E_E2_IDP96932.106_T7S.seq 1788 0.0 2_08E_E2_IDP96932.106_T7term.seq 1458 0.0 Q F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 55 CAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTG 144 1 15 ................-.......................................................------------------ 85 W S H P Q F E K G T E N L Y F Q S N A R A L V E Q Q L Q Y T Query_1 145 TGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCGGGCGTTGGTTGAACAGCAATTACAGTACACA 234 0 1 ................................. 33 1 86 ------------------------------............................................................ 145 L C V S L N K A T H G D I F N A V A L A I R H F Q Q D H F L Query_1 235 TTGTGTGTTAGCTTAAATAAAGCTACGCATGGCGATATTTTTAACGCGGTGGCGCTGGCCATTCGTCATTTTCAACAGGACCATTTTTTG 324 0 34 .......................................................................................... 123 1 146 .......................................................................................... 235 L S Q K R Q R E E R K K R V Y Y L S M E F L L G Q S L R N N Query_1 325 TTGAGCCAGAAGCGGCAACGTGAAGAACGTAAAAAGCGCGTTTACTATCTGTCGATGGAGTTTTTACTGGGACAGTCGCTGCGTAATAAC 414 0 124 .......................................................................................... 213 1 236 .......................................................................................... 325 L L N M N L L A E M H Q V V N D L G F D L D H L L D E E P D Query_1 415 CTTTTGAATATGAATTTACTGGCGGAGATGCACCAGGTGGTGAATGACCTGGGGTTTGATCTTGACCACCTTCTTGACGAAGAGCCCGAC 504 0 214 .......................................................................................... 303 1 326 .......................................................................................... 415 A A L G N G G L G R L A A C F I D S M A T L D I A A S G H G Query_1 505 GCCGCGCTGGGTAACGGAGGGTTAGGGCGCTTAGCCGCCTGTTTTATCGATTCGATGGCCACCCTTGATATTGCCGCATCCGGGCATGGC 594 0 304 .......................................................................................... 393 0 1446 ........... 1436 1 416 .......................................................................................... 505 I K Y E Y G L F R Q S F Q N D Q Q I E H P D D W H S M H S P Query_1 595 ATCAAATACGAATATGGTCTTTTTCGCCAATCCTTTCAAAATGACCAGCAGATCGAGCATCCGGATGACTGGCATTCCATGCACTCCCCG 684 0 394 .......................................................................................... 483 1 506 .......................................................................................... 595 W L V E H H A Q Q I L I P L G G Y I E H S E D V D G N Y N P Query_1 685 TGGCTGGTCGAACATCACGCTCAGCAAATCCTGATCCCGCTGGGAGGCTATATTGAACACAGCGAAGATGTTGATGGCAATTACAATCCA 774 0 484 .......................................................................................... 573 0 1202 ...A..G........... 1185 1 596 .......................................................................................... 685 M W L G W K T I I G I P H D Y F V S G Y R D T T T N K L R L Query_1 775 ATGTGGCTGGGCTGGAAAACGATTATCGGTATACCCCATGACTATTTCGTTTCCGGTTACCGGGATACGACGACCAACAAGCTGCGGCTT 864 0 574 .......................................................................................... 663 1 686 .......................................................................................... 775 Y S A V A S D S F N I H I F N R G D Y I K A V S D K I A S E Query_1 865 TATAGCGCGGTGGCCTCTGACTCCTTTAATATCCATATTTTCAATCGCGGTGATTATATCAAGGCGGTGAGTGATAAAATCGCGTCGGAG 954 0 664 .......................................................................................... 753 1 776 .......................................................................................... 865 N I S K I L Y P S D E V L T G K E L R L T Q E Y F L V A C Query_1 955 -AATATTTCAAAAATACTCTAT-CCATCCGATGAGGTGCTGACCGGAAAAGAGCTGCGGCTGACGCAAGAGTATTTTCTGGT-GGCCTGT 1041 0 754 -.....................-...........................................................-....... 840 1 866 A.....................C...........................................................G....... 955 T L R D I F R D Y A E V N D D I T F L P Q H V A I Q L N D Query_1 1042 ACGCTGCGGGATATATTTCGCGAC-TATGCCGAGG-TTAATGACGATATTAC-TTTCCTGCCGCAGCATGTGGCGATTCAGCTGAATGAC 1128 0 841 ........................-..........-................-..................................... 927 0 924 ............ 913 1 956 .......-................N..........T................T............N........................ 1044 1 1041 ............ 1030 T H P A L A V V E L M R I L V D E Y R L P W E Q A W Query_1 1129 ACCCAT-CCGGC-ATTAGCGGTGGTGGAGC-TGATGCGTAT-CCTCGTCG-ATGAAT-ACCGGC--TGCCC-TGGGAGC-AGGCGTGGG- 1207 0 928 ......-.....-.................-..........-........-......-......--.....-.......-.........- 1006 0 2327 .....-.....-. 2317 1 1045 ......C.....N.........N.......T..........C........A......NN.....CTN....T...A...A..C......A 1134 E I T Q N T C A Y T N H T L M P E A L E T W P V T L F Query_1 1208 AGA-TAACGCAAAACACCTGTGCC-TACAC--GAATCATACGTT-GATG-CCGGAAG-CGCTGGAGACCTG-GCCGGTGACGCTGTTTGA 1289 0 1007 ...-....................-.....--............-....-.......-.............-.................. 1088 1 1135 ...N....N.....N.....NN..T.....CNN.N...NNNN..N....T..N....C.....A.N.....NN.N..... 1214 E K L L P R H L Q I I F E I N H R F L E E V A R R W P D K N Query_1 1290 AAAACTGCTGCCGCGTCATTTGCAAATTATATTTGAGATCAACCATCGTTTCCTTGAAGAGGTGGCCCGGCGCTGGCCGGATAAAAATCA 1379 0 1089 .......................................................................................... 1178 H L L S S L S L I D E H R D K Q I R M A N L A I V G S H R V Query_1 1380 TCTGTTATCTTCGCTGTCTTTAATCGACGAACATCGCGATAAGCAAATCCGTATGGCCAATCTGGCGATTGTCGGTAGCCATCGCGTGAA 1469 0 1179 .......................................................................................... 1268 0 552 ...........G..C... 535 1 664 ...........G..C... 647 N G V A W L H S E L V K K Q L V P D F Y F M T P E K F I N Q Query_1 1470 CGGGGTAGCATGGCTGCATTCGGAACTGGTCAAGAAACAACTGGTGCCCGACTTCTATTTTATGACCCCGGAGAAATTCATTAATCAGAC 1559 0 1269 .......................................................................................... 1358 T N G V T P R R W V Q Q A N P G L A A F L H Q Q L G E G W P Query_1 1560 CAACGGCGTCACGCCGCGACGCTGGGTGCAGCAGGCTAACCCCGGCCTGGCCGCTTTTTTGCACCAACAGCTGGGGGAAGGGTGGCCAAC 1649 0 1359 .......................................................................................... 1448 0 369 ........... 359 1 481 ........... 471 T D L P L L S S L E S L A D D T A V I D N I R A I K F A N K Query_1 1650 CGATTTACCCTTGCTGTCCAGTCTGGAAAGCCTGGCCGATGATACTGCTGTTATTGATAATATCCGGGCCATTAAATTTGCCAACAAAAC 1739 0 1449 .......................................................................................... 1538 T A L S Q L V A Q R Y G I A L D P L A M F D C Q V K R I H E Query_1 1740 CGCGCTCAGCCAGCTGGTCGCTCAACGCTATGGCATCGCCCTCGATCCGCTGGCGATGTTTGATTGTCAGGTCAAACGTATTCATGAATA 1829 0 1539 .......................................................................................... 1628 0 1628 ............ 1617 Y K R Q L L N I L H V I A L Y L D I K E T G K T I A P K V H Query_1 1830 TAAGCGTCAGTTGCTCAATATTCTTCATGTGATCGCGCTTTATCTGGATATCAAAGAGACAGGGAAAA-CTATCGCGCCTAAGGTGCA-T 1917 0 1629 ....................................................................-...................-. 1716 2 841 .....................A.........G.........A. 799 L F A G K A A P G Y R M A K L I I Q L I N T V A R K I N R D Query_1 1918 TTGTTTGCGGGTAAAGCGGCGCCGGGCTATCGGATGGCGAAGCTGATTATTCAGTTGATTAATACCGTCGCCCGGAAAATTAACCGCGAC 2007 0 1717 .......................................................................................... 1806 2 798 .......................................................................................... 709 P Q V A G Q L K V V F L E D Y K V S L A E K I I P A T D L S Query_1 2008 CCGCAGGTTGCCGGGCAGCTTAAAGTGGTTTTCCTCGAAGATTATAAAGTCTCGCTGGCGGAAAAAATCATTCCGGCCACAGACCTCTCC 2097 0 1807 .......................................................................................... 1896 2 708 .......................................................................................... 619 E Q I S T A G T E A S G T S N M K F A M N G A L T I G T Y D Query_1 2098 GAGCAAATTTCCACCGCCGGGACCGAGGCGTCCGGCACCAGCAATATGAAGTTTGCCATGAACGGCGCGTTAACCATCGGCACCTATGAT 2187 0 1897 .......................................................................................... 1986 2 618 .......................................................................................... 529 G A N I E I R E A V G I D N F Y L F G A V A E E I E E S R R Query_1 2188 GGGGCGAATATCGAAATTCGCGAGGCCGTGGGGATCGATAATTTTTATCTGTTTGGCGCCGTGGCGGAAGAGATTGAAGAGAGCCGGCGC 2277 0 1987 .......................................................................................... 2076 2 528 .......................................................................................... 439 L G H H H N F Y H Q N P A M A R V V D T L V S G L F T S D R Query_1 2278 CTTGGGCATCACCATAATTTCTATCATCAGAACCCGGCTATGGCGCGTGTTGTCGATACCCTCGTGTCTGGGTTGTTCACCAGCGATCGT 2367 0 2077 .......................................................................................... 2166 2 438 .......................................................................................... 349 E L F A P L H H M L T E G D H Y C H L L D F D S Y C Q A Q R Query_1 2368 GAGCTGTTTGCACCCCTTCACCACATGTTGACTGAGGGCGATCATTACTGTCATTTACTGGATTTCGACAGTTATTGTCAGGCCCAGCGG 2457 0 2167 .......................................................................................... 2256 2 348 .......................................................................................... 259 Q A M A D F E L P E Q W H R R A L L N I C R M G E F S S D R Query_1 2458 CAGGCGATGGCCGATTTTGAACTGCCGGAACAATGGCACCGTCGGGCCTTACTCAATATTTGCCGGATGGGCGAATTTTCCAGCGACAGA 2547 0 2257 .......................................................................................... 2346 0 939 ........... 929 2 258 .......................................................................................... 169 T I R G Y A R D I W G I * H W K W I T D P N S S S V D K L A Query_1 2548 ACCATACGTGGCTACGCCCGGGATATTTGGGGTATCTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCG 2637 0 2347 .................................... 2382 2 168 .......................................................................................... 79 A A L E H H H H H H * D P A A N K A R K E Query_1 2638 GCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAA 2700 2 78 .......................................................NN..A... 16 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 12373590 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 4,518 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96932.107 (IDP96932.1-808.F..pMCSG53)
MPDINPQNIKELRALVEQQLQYTLCVSLNKATHGDIFNAVALAIRHFQQDHFLLSQKRQREERKKRVYYLSMEFLLGQSLRNNLLNMNLLAEMHQVVNDLGFDLDHLLDEEPDAALGNGGLGRLAACFIDSMATLDIAASGHGIKYEYGLFRQSFQNDQQIEHPDDWHSMHSPWLVEHHAQQILIPLGGYIEHSEDVDGNYNPMWLGWKTIIGIPHDYFVSGYRDTTTNKLRLYSAVASDSFNIHIFNRGDYIKAVSDKIASENISKILYPSDEVLTGKELRLTQEYFLVACTLRDIFRDYAEVNDDITFLPQHVAIQLNDTHPALAVVELMRILVDEYRLPWEQAWEITQNTCAYTNHTLMPEALETWPVTLFEKLLPRHLQIIFEINHRFLEEVARRWPDKNHLLSSLSLIDEHRDKQIRMANLAIVGSHRVNGVAWLHSELVKKQLVPDFYFMTPEKFINQTNGVTPRRWVQQANPGLAAFLHQQLGEGWPTDLPLLSSLESLADDTAVIDNIRAIKFANKTALSQLVAQRYGIALDPLAMFDCQVKRIHEYKRQLLNILHVIALYLDIKETGKTIAPKVHLFAGKAAPGYRMAKLIIQLINTVARKINRDPQVAGQLKVVFLEDYKVSLAEKIIPATDLSEQISTAGTEASGTSNMKFAMNGALTIGTYDGANIEIREAVGIDNFYLFGAVAEEIEESRRLGHHHNFYHQNPAMARVVDTLVSGLFTSDRELFAPLHHMLTEGDHYCHLLDFDSYCQAQRQAMADFELPEQWHRRALLNICRMGEFSSDRTIRGYARDIWGITS
ATGCCTGACATTAACCCGCAAAATATCAAAGAATTACGGGCGTTGGTTGAACAGCAATTACAGTACACATTGTGTGTTAGCTTAAATAAAGCTACGCATGGCGATATTTTTAACGCGGTGGCGCTGGCCATTCGTCATTTTCAACAGGACCATTTTTTGTTGAGCCAGAAGCGGCAACGTGAAGAACGTAAAAAGCGCGTTTACTATCTGTCGATGGAGTTTTTACTGGGACAGTCGCTGCGTAATAACCTTTTGAATATGAATTTACTGGCGGAGATGCACCAGGTGGTGAATGACCTGGGGTTTGATCTTGACCACCTTCTTGACGAAGAGCCCGACGCCGCGCTGGGTAACGGAGGGTTAGGGCGCTTAGCCGCCTGTTTTATCGATTCGATGGCCACCCTTGATATTGCCGCATCCGGGCATGGCATCAAATACGAATATGGTCTTTTTCGCCAATCCTTTCAAAATGACCAGCAGATCGAGCATCCGGATGACTGGCATTCCATGCACTCCCCGTGGCTGGTCGAACATCACGCTCAGCAAATCCTGATCCCGCTGGGAGGCTATATTGAACACAGCGAAGATGTTGATGGCAATTACAATCCAATGTGGCTGGGCTGGAAAACGATTATCGGTATACCCCATGACTATTTCGTTTCCGGTTACCGGGATACGACGACCAACAAGCTGCGGCTTTATAGCGCGGTGGCCTCTGACTCCTTTAATATCCATATTTTCAATCGCGGTGATTATATCAAGGCGGTGAGTGATAAAATCGCGTCGGAGAATATTTCAAAAATACTCTATCCATCCGATGAGGTGCTGACCGGAAAAGAGCTGCGGCTGACGCAAGAGTATTTTCTGGTGGCCTGTACGCTGCGGGATATATTTCGCGACTATGCCGAGGTTAATGACGATATTACTTTCCTGCCGCAGCATGTGGCGATTCAGCTGAATGACACCCATCCGGCATTAGCGGTGGTGGAGCTGATGCGTATCCTCGTCGATGAATACCGGCTGCCCTGGGAGCAGGCGTGGGAGATAACGCAAAACACCTGTGCCTACACGAATCATACGTTGATGCCGGAAGCGCTGGAGACCTGGCCGGTGACGCTGTTTGAAAAACTGCTGCCGCGTCATTTGCAAATTATATTTGAGATCAACCATCGTTTCCTTGAAGAGGTGGCCCGGCGCTGGCCGGATAAAAATCATCTGTTATCTTCGCTGTCTTTAATCGACGAACATCGCGATAAGCAAATCCGTATGGCCAATCTGGCGATTGTCGGTAGCCATCGCGTGAACGGGGTAGCATGGCTGCATTCGGAACTGGTCAAGAAACAACTGGTGCCCGACTTCTATTTTATGACCCCGGAGAAATTCATTAATCAGACCAACGGCGTCACGCCGCGACGCTGGGTGCAGCAGGCTAACCCCGGCCTGGCCGCTTTTTTGCACCAACAGCTGGGGGAAGGGTGGCCAACCGATTTACCCTTGCTGTCCAGTCTGGAAAGCCTGGCCGATGATACTGCTGTTATTGATAATATCCGGGCCATTAAATTTGCCAACAAAACCGCGCTCAGCCAGCTGGTCGCTCAACGCTATGGCATCGCCCTCGATCCGCTGGCGATGTTTGATTGTCAGGTCAAACGTATTCATGAATATAAGCGTCAGTTGCTCAATATTCTTCATGTGATCGCGCTTTATCTGGATATCAAAGAGACAGGGAAAACTATCGCGCCTAAGGTGCATTTGTTTGCGGGTAAAGCGGCGCCGGGCTATCGGATGGCGAAGCTGATTATTCAGTTGATTAATACCGTCGCCCGGAAAATTAACCGCGACCCGCAGGTTGCCGGGCAGCTTAAAGTGGTTTTCCTCGAAGATTATAAAGTCTCGCTGGCGGAAAAAATCATTCCGGCCACAGACCTCTCCGAGCAAATTTCCACCGCCGGGACCGAGGCGTCCGGCACCAGCAATATGAAGTTTGCCATGAACGGCGCGTTAACCATCGGCACCTATGATGGGGCGAATATCGAAATTCGCGAGGCCGTGGGGATCGATAATTTTTATCTGTTTGGCGCCGTGGCGGAAGAGATTGAAGAGAGCCGGCGCCTTGGGCATCACCATAATTTCTATCATCAGAACCCGGCTATGGCGCGTGTTGTCGATACCCTCGTGTCTGGGTTGTTCACCAGCGATCGTGAGCTGTTTGCACCCCTTCACCACATGTTGACTGAGGGCGATCATTACTGTCATTTACTGGATTTCGACAGTTATTGTCAGGCCCAGCGGCAGGCGATGGCCGATTTTGAACTGCCGGAACAATGGCACCGTCGGGCCTTACTCAATATTTGCCGGATGGGCGAATTTTCCAGCGACAGAACCATACGTGGCTACGCCCGGGATATTTGGGGTATCACCAGC
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 4,923 total letters Query= IDP96932.107 Length=2829 Score E Sequences producing significant alignments: (Bits) Value 0_wt 4372 0.0 2_12E_E9_IDP96932.107_T7term.seq 1966 0.0 1_09E_E9_IDP96932.107_T7S.seq 1837 0.0 Q F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 55 CAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTG 144 1 8 ..T.............-.......................................................------------------ 78 W S H P Q F E K G T E N L Y F Q S N A M P D I N P Q N I K E Query_1 145 TGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGCCTGACATTAACCCGCAAAATATCAAAGAA 234 0 1 ................................. 33 1 79 ------------------------------............................................................ 138 L R A L V E Q Q L Q Y T L C V S L N K A T H G D I F N A V A Query_1 235 TTACGGGCGTTGGTTGAACAGCAATTACAGTACACATTGTGTGTTAGCTTAAATAAAGCTACGCATGGCGATATTTTTAACGCGGTGGCG 324 0 34 .......................................................................................... 123 1 139 .......................................................................................... 228 L A I R H F Q Q D H F L L S Q K R Q R E E R K K R V Y Y L S Query_1 325 CTGGCCATTCGTCATTTTCAACAGGACCATTTTTTGTTGAGCCAGAAGCGGCAACGTGAAGAACGTAAAAAGCGCGTTTACTATCTGTCG 414 0 124 .......................................................................................... 213 1 229 .......................................................................................... 318 M E F L L G Q S L R N N L L N M N L L A E M H Q V V N D L G Query_1 415 ATGGAGTTTTTACTGGGACAGTCGCTGCGTAATAACCTTTTGAATATGAATTTACTGGCGGAGATGCACCAGGTGGTGAATGACCTGGGG 504 0 214 .......................................................................................... 303 1 319 .......................................................................................... 408 F D L D H L L D E E P D A A L G N G G L G R L A A C F I D S Query_1 505 TTTGATCTTGACCACCTTCTTGACGAAGAGCCCGACGCCGCGCTGGGTAACGGAGGGTTAGGGCGCTTAGCCGCCTGTTTTATCGATTCG 594 0 304 .......................................................................................... 393 1 409 .......................................................................................... 498 M A T L D I A A S G H G I K Y E Y G L F R Q S F Q N D Q Q I Query_1 595 ATGGCCACCCTTGATATTGCCGCATCCGGGCATGGCATCAAATACGAATATGGTCTTTTTCGCCAATCCTTTCAAAATGACCAGCAGATC 684 0 394 .......................................................................................... 483 0 1482 ........... 1472 1 499 .......................................................................................... 588 E H P D D W H S M H S P W L V E H H A Q Q I L I P L G G Y I Query_1 685 GAGCATCCGGATGACTGGCATTCCATGCACTCCCCGTGGCTGGTCGAACATCACGCTCAGCAAATCCTGATCCCGCTGGGAGGCTATATT 774 0 484 .......................................................................................... 573 0 1238 ... 1236 1 589 .......................................................................................... 678 E H S E D V D G N Y N P M W L G W K T I I G I P H D Y F V S Query_1 775 GAACACAGCGAAGATGTTGATGGCAATTACAATCCAATGTGGCTGGGCTGGAAAACGATTATCGGTATACCCCATGACTATTTCGTTTCC 864 0 574 .......................................................................................... 663 0 1235 A..G........... 1221 1 679 .......................................................................................... 768 G Y R D T T T N K L R L Y S A V A S D S F N I H I F N R G D Query_1 865 GGTTACCGGGATACGACGACCAACAAGCTGCGGCTTTATAGCGCGGTGGCCTCTGACTCCTTTAATATCCATATTTTCAATCGCGGTGAT 954 0 664 .......................................................................................... 753 1 769 ......................................................................................N... 858 Y I K A V S D K I A S E N I S K I L Y P S D E V L T G K E L Query_1 955 TATATCAAGGCGGTGAGTGATAAAATCGCGTCGGAGAATATTTCAAAAATACTCTATCCATCCGATGAGGTGCTGACCGGAAAAGAGCTG 1044 0 754 .......................................................................................... 843 1 859 ...........................................................N.............................. 948 R L T Q E Y F L V A C T L R D I F R D Y A E V N D D I T F Query_1 1045 CGGCTGACGCAAGAGTATTTTCTGGTGGCCTGTACGCTGCGGGA-TATATTTCGCGACTATGCCGAGGTTAATGACGATATTACTTTCCT 1133 0 844 ............................................-............................................. 932 1 949 ..............-N............................NN...-...............................N........ 1036 L P Q H V A I Q L N D T H P A L A V V E L M R I L V D E Y Query_1 1134 GCCGCAGCATGTGGCGATTCAGCTGAATGACACCCATCCGGCATTAGCGGTGGTGGAGC-TG-ATGCGTAT-CCTCGTCG-ATGAATA-C 1218 0 933 ...........................................................-..-........-........-.......-. 1017 0 960 ............ 949 0 2363 ........... 2353 1 1037 ......N............................N.......A....-....N.....N..A........C..NNN...A.......N. 1125 1 1064 ............ 1053 R L P W E Q A W E I T Q N T C A Y T N H T L M P E A L E Query_1 1219 CGGCTGCCCTGGGAGCAGG--CGTGGGAGATAACGCAAAACACC-TGTGCCTACACG-AATCATACGTTGATGCCGGAAGCGCTGGAGAC 1304 0 1018 ...................--.......................-............-................................ 1103 1 1126 .....N........N....NN.NG......N.NNAA..T.....N.NGN.....C..A....... 1190 T W P V T L F E K L L P R H L Q I I F E I N H R F L E E V A Query_1 1305 CTGGCCGGTGACGCTGTTTGAAAAACTGCTGCCGCGTCATTTGCAAATTATATTTGAGATCAACCATCGTTTCCTTGAAGAGGTGGCCCG 1394 0 1104 .......................................................................................... 1193 R R W P D K N H L L S S L S L I D E H R D K Q I R M A N L A Query_1 1395 GCGCTGGCCGGATAAAAATCATCTGTTATCTTCGCTGTCTTTAATCGACGAACATCGCGATAAGCAAATCCGTATGGCCAATCTGGCGAT 1484 0 1194 .......................................................................................... 1283 0 588 ...........G..C... 571 1 693 ...........G..C... 676 I V G S H R V N G V A W L H S E L V K K Q L V P D F Y F M T Query_1 1485 TGTCGGTAGCCATCGCGTGAACGGGGTAGCATGGCTGCATTCGGAACTGGTCAAGAAACAACTGGTGCCCGACTTCTATTTTATGACCCC 1574 0 1284 .......................................................................................... 1373 2 1207 ......N.....NN.....N..G.N.....N...-...N...- 1167 P E K F I N Q T N G V T P R R W V Q Q A N P G L A A F L H Q Query_1 1575 GGAGAAATTCATTAATCAGACCAACGGCGTCACGCCGCGACGCTGGGTGCAGCAGGCTAACCCCGGCCTGGCCGCTTTTTTGCACCAACA 1664 0 1374 .......................................................................................... 1463 2 1166 ......N...N.--....NN....N.......NN.-.....N.....................N.--...N.-.......-..N.--... 1086 Q L G E G W P T D L P L L S S L E S L A D D T A V I D N I R Query_1 1665 GCTGGGGGAAGGGTGGCCAACCGATTTACCCTTGCTGTCCAGTCTGGAAAGCCTGGCCGATGATACTGCTGTTATTGATAATATCCGGGC 1754 0 1464 .......................................................................................... 1553 0 405 ........... 395 2 1085 .......-.....NT.N.N.............-....N........-........................................... 999 A I K F A N K T A L S Q L V A Q R Y G I A L D P L A M F D C Query_1 1755 CATTAAATTTGCCAACAAAACCGCGCTCAGCCAGCTGGTCGCTCAACGCTATGGCATCGCCCTCGATCCGCTGGCGATGTTTGATTGTCA 1844 0 1554 .......................................................................................... 1643 2 998 .........-.........-.............................................................-........ 912 Q V K R I H E Y K R Q L L N I L H V I A L Y L D I K E T G K Query_1 1845 GGTCAAACGTATTCATGAATATAAGCGTCAGTTGCTCAATATTCTTCATGTGATCGCGCTTTATCTGGATATCAAAGAGACAGGGAAAAC 1934 0 1644 .......................................................................................... 1733 0 1664 ............ 1653 2 911 .......................................................................................... 822 T I A P K V H L F A G K A A P G Y R M A K L I I Q L I N T V Query_1 1935 TATCGCGCCTAAGGTGCATTTGTTTGCGGGTAAAGCGGCGCCGGGCTATCGGATGGCGAAGCTGATTATTCAGTTGATTAATACCGTCGC 2024 0 1734 .......................................................................................... 1823 2 821 .......................................................................................... 732 A R K I N R D P Q V A G Q L K V V F L E D Y K V S L A E K I Query_1 2025 CCGGAAAATTAACCGCGACCCGCAGGTTGCCGGGCAGCTTAAAGTGGTTTTCCTCGAAGATTATAAAGTCTCGCTGGCGGAAAAAATCAT 2114 0 1824 .......................................................................................... 1913 2 731 .......................................................................................... 642 I P A T D L S E Q I S T A G T E A S G T S N M K F A M N G A Query_1 2115 TCCGGCCACAGACCTCTCCGAGCAAATTTCCACCGCCGGGACCGAGGCGTCCGGCACCAGCAATATGAAGTTTGCCATGAACGGCGCGTT 2204 0 1914 .......................................................................................... 2003 2 641 .......................................................................................... 552 L T I G T Y D G A N I E I R E A V G I D N F Y L F G A V A E Query_1 2205 AACCATCGGCACCTATGATGGGGCGAATATCGAAATTCGCGAGGCCGTGGGGATCGATAATTTTTATCTGTTTGGCGCCGTGGCGGAAGA 2294 0 2004 .......................................................................................... 2093 2 551 .......................................................................................... 462 E I E E S R R L G H H H N F Y H Q N P A M A R V V D T L V S Query_1 2295 GATTGAAGAGAGCCGGCGCCTTGGGCATCACCATAATTTCTATCATCAGAACCCGGCTATGGCGCGTGTTGTCGATACCCTCGTGTCTGG 2384 0 2094 .......................................................................................... 2183 2 461 .......................................................................................... 372 G L F T S D R E L F A P L H H M L T E G D H Y C H L L D F D Query_1 2385 GTTGTTCACCAGCGATCGTGAGCTGTTTGCACCCCTTCACCACATGTTGACTGAGGGCGATCATTACTGTCATTTACTGGATTTCGACAG 2474 0 2184 .......................................................................................... 2273 2 371 .......................................................................................... 282 S Y C Q A Q R Q A M A D F E L P E Q W H R R A L L N I C R M Query_1 2475 TTATTGTCAGGCCCAGCGGCAGGCGATGGCCGATTTTGAACTGCCGGAACAATGGCACCGTCGGGCCTTACTCAATATTTGCCGGATGGG 2564 0 2274 .......................................................................................... 2363 0 975 ........... 965 2 281 .......................................................................................... 192 G E F S S D R T I R G Y A R D I W G I T S * H W K W I T D P Query_1 2565 CGAATTTTCCAGCGACAGAACCATACGTGGCTACGCCCGGGATATTTGGGGTATCACCAGCTAACATTGGAAGTGGATAACGGATCCGAA 2654 0 2364 ............................................................. 2424 2 191 .......................................................................................... 102 N S S S V D K L A A A L E H H H H H H * D P A A N K A R K Query_1 2655 TTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccaccaccacTGAGATCCGGCTGCTAACAAAGCCCGAAAG 2739 2 101 ..................................................................................... 17 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 13700064 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 4,923 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96938.104 (IDP96938.1-326.P(328)..pMCSG53)
MSKMIAVTMGDPAGIGPEIIIKSLAEGALSGAPVVVVGCAQTLRRILALNITPRAELRIIDHPAEASFSPATINVIDEPLSDPQGLRPGEVQAQAGDLAFRCIRRATALALEGAVAAIATAPLNKEALHLAGHAYPGHTELLAHLTQTTDYAMVLYTEKLKVIHITTHISLRQFLDTLNQPRIETVIGVADRFLRRVGYPRPRIAVAGVNPHAGENGLFGDEEIRIVAPAVAAMRAKGVEVTGPCPPDTVFMQCHEGMYDMVVAMYHDQGHIPLKLLGFYDGVNITAGLPFIRTSADHGTAFDIAWTGKAKSESMATSIELAMHIA
GTGTCTAAGATGATTGCAGTGACAATGGGCGACCCGGCAGGGATTGGTCCGGAGATTATTATCAAATCCCTCGCCGAGGGGGCGCTGAGCGGCGCGCCGGTGGTGGTGGTGGGCTGCGCGCAGACGCTGCGGCGGATCCTGGCGCTAAATATCACACCGCGGGCGGAGCTGCGCATTATTGACCATCCCGCCGAGGCGAGTTTTTCCCCAGCGACTATCAACGTCATTGATGAACCACTCAGCGACCCGCAGGGGCTGCGCCCTGGCGAAGTCCAGGCCCAGGCAGGGGATCTCGCGTTTCGCTGCATCAGGCGGGCGACCGCGCTGGCGCTGGAGGGCGCGGTCGCGGCCATCGCTACCGCGCCGCTGAATAAAGAGGCGCTGCATCTCGCCGGGCACGCTTATCCCGGCCATACCGAACTGCTGGCCCACCTGACGCAGACTACCGATTACGCCATGGTGCTCTATACCGAGAAGCTTAAGGTTATCCATATCACTACCCATATTTCCTTGCGCCAGTTCCTCGATACCCTCAACCAGCCGCGGATTGAGACGGTGATTGGCGTGGCGGATCGCTTTCTGCGCCGCGTCGGCTATCCACGTCCGCGCATCGCGGTCGCCGGCGTTAACCCGCACGCCGGGGAGAACGGCCTGTTTGGCGATGAAGAGATCCGTATCGTCGCCCCGGCGGTCGCCGCCATGCGGGCGAAGGGAGTGGAGGTGACCGGCCCCTGTCCGCCGGACACGGTGTTTATGCAGTGTCACGAAGGCATGTACGACATGGTGGTGGCGATGTATCACGACCAGGGGCATATTCCGCTGAAGCTGCTGGGATTCTATGACGGGGTGAATATTACCGCCGGGCTGCCGTTTATCCGTACCTCCGCGGATCATGGCACTGCGTTTGATATTGCCTGGACAGGTAAAGCGAAGTCTGAGAGCATGGCAACCTCCATTGAGCTCGCCATGCACATCGCG
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,173 total letters Query= IDP96938.104 Length=1383 Score E Sequences producing significant alignments: (Bits) Value 0_wt 1764 0.0 2_08D_D2_IDP96938.104_T7term.seq 1730 0.0 1_02D_D2_IDP96938.104_T7S.seq 1370 0.0 Q F P S R N N F V * L * E G D I H M H H H H H H S S G V D Query_1 55 CAATTCCCCTC-TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCT 143 1 16 ...........C.............................................................----------------- 88 L W S H P Q F E K G T E N L Y F Q S N A V S K M I A V T Query_1 144 GTGGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAAT-CCAA-TGCCGTG-TCTAAG-ATGATT-GCAGTGAC-AAT 227 0 1 ...-......-......-........-... 26 2 1162 .....NNN.N....T....G.....G.G.T...NN....N.N..G.G...C... 1109 1 89 -------------------------------...................-....-.......-......-......-........-... 141 M G D P A G I G P E I I I K S L A E G A L S G A P V Query_1 228 GGGCGACCCGG-CAGGGATTGG---TCCGGAG--ATTATTAT-CAAATCCCT-CGCCGAGGGGGCGCTGAG-CGGC-GCGCCGGTGG--T 305 0 27 ...........-..........---.......--........-.........-..................-....-..........--. 104 2 1108 ...N.......G.....N..T.GNT.......GN......N.N...T.....T.................NN....C.......NN.GN. 1019 2 57 .....--. 62 2 56 ...--. 59 1 142 ...........-..........---.......--........-.........-..................-....-..........--. 219 V V V G C A Q T L R R I L A L N I T P R A E L R I I D H Query_1 306 GGTGGT-GGGCT-GCGCGC-AGACGCTGCGGCGGATCC-TGGCGCTAAA-TATCACACCGCGGGCGGAGCT-GCGCATTATTGACCATCC 389 0 105 ......-.....-......-..................-..........-.....................-.................. 188 2 1018 ......T.....T..N...C.N................CN.........A........N..........G.T.................. 929 2 63 ......-. 69 2 60 ......-.. 67 1 220 ......-.....-......-..................-..........-.....................-.................. 303 P A E A S F S P A T I N V I D E P L S D P Q G L R P G E V Query_1 390 CGCCGAGGCGAGTTTTT-CCCCAGCGACTATCAACGTCATTGATGAACCACTCAGCGACCC-GCAGGGGCTGCGCCC-TGGCGAAGTCCA 476 0 189 .................-...........................................-...............-............ 275 2 928 .................T....N......N...............................C.....A.N.......C............ 839 1 304 .................-...........................................-...............-............ 390 Q A Q A G D L A F R C I R R A T A L A L E G A V A A I A T A Query_1 477 GGCCCAGGCAGGGGATCTCGCGTTTCGCTGCATCAGGCGGGCGACCGCGCTGGCGCTGGAG-GGCGCGGTCGCGGCCATCGCTACCGCGC 565 0 276 .............................................................-............................ 364 2 838 .-...........................................................A............................ 750 1 391 .............................................................-............................ 479 1 820 ........... 810 P L N K E A L H L A G H A Y P G H T E L L A H L T Q T T D Y Query_1 566 CGCTGAATAAAGAGGCGCTGCATCTCGCCGGGCACGCTTATCCCGGCCATACCGAACTGCTGGCCCACCTGACGCAGACTACCGATTACG 655 0 365 .......................................................................................... 454 2 749 .......................................................................................... 660 1 480 .......................................................................................... 569 A M V L Y T E K L K V I H I T T H I S L R Q F L D T L N Q P Query_1 656 CCATGGTGCTCTATACCGAGAAGCTTAAGGTTATCCATATCACTACCCATATTTCCTTGCGCCAGTTCCTCGATACCCTCAACCAGCCGC 745 0 455 .......................................................................................... 544 2 659 .......................................................................................... 570 1 570 ...............N.................................................................N........ 659 R I E T V I G V A D R F L R R V G Y P R P R I A V A G V N Query_1 746 GGATTGAGACGGTGATTGGCGTGGCGGATCGCTTTCTGCGCCGCGTCGGCTAT-CCACGT-CCGCGCATCGCGGTCGCCGGCGTTAACCC 833 0 545 .....................................................-......-............................. 632 2 569 .....................................................-......-............................. 482 1 660 .....................................................C......C.............N............... 749 P H A G E N G L F G D E E I R I V A P A V A A M R A K G Query_1 834 GCACGCC-GGGGAGAACGG-CCTGTTT-GGCGATGAAGAGATCCGT-ATCGTCG-CCCCGGCGGTCG-CCGCCATGCGGGCGAAGGGAGT 917 0 633 .......-...........-.......-..................-.......-............-...................... 716 2 481 .......-...........-.......-..................-.......-............-...................... 398 1 750 .......G...........C.......G..................N.......C............C...N.................. 839 V E V T G P C P P D T V F M Q C H E G M Y D M V V A M Y H D Query_1 918 GGAGGTGA-CCGGCCCCTGTCCG-CCGGACACGGTGTTTATGCAGTGTCACGAAGGCATGTACGACATGGTGGTGGCGATGTATCACGAC 1005 0 717 ........-..............-.................................................................. 804 2 397 ........-..............-.................................................................. 310 1 840 ........C..............T................... 882 Q G H I P L K L L G F Y D G V N I T A G L P F I R T S A D H Query_1 1006 CAGGGGCATATTCCGCTGAAGCTGCTGGGATTCTATGACGGGGTGAATATTACCGCCGGGCTGCCGTTTATCCGTACCTCCGCGGATCAT 1095 0 805 .......................................................................................... 894 2 309 .......................................................................................... 220 G T A F D I A W T G K A K S E S M A T S I E L A M H I A * H Query_1 1096 GGCACTGCGTTTGATATTGCCTGGACAGGTAAAGCGAAGTCTGAGAGCATGGCAACCTCCATTGAGCTCGCCATGCACATCGCGTAACAT 1185 0 895 .................................................................................... 978 2 219 .......................................................................................... 130 W K W I T D P N S S S V D K L A A A L E H H H H H H * D P Query_1 1186 TGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccacc-accacTGAGATCCGGC 1274 2 129 .........................................................................A................ 40 A A N K A R Query_1 1275 TGCTAACAAAGCCCGAAA 1292 2 39 .................. 22 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4271875 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 3,173 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2
Clone ID: IDP96938.105 (IDP96938.1-328.F..pMCSG53)
MSKMIAVTMGDPAGIGPEIIIKSLAEGALSGAPVVVVGCAQTLRRILALNITPRAELRIIDHPAEASFSPATINVIDEPLSDPQGLRPGEVQAQAGDLAFRCIRRATALALEGAVAAIATAPLNKEALHLAGHAYPGHTELLAHLTQTTDYAMVLYTEKLKVIHITTHISLRQFLDTLNQPRIETVIGVADRFLRRVGYPRPRIAVAGVNPHAGENGLFGDEEIRIVAPAVAAMRAKGVEVTGPCPPDTVFMQCHEGMYDMVVAMYHDQGHIPLKLLGFYDGVNITAGLPFIRTSADHGTAFDIAWTGKAKSESMATSIELAMHIAQE
GTGTCTAAGATGATTGCAGTGACAATGGGCGACCCGGCAGGGATTGGTCCGGAGATTATTATCAAATCCCTCGCCGAGGGGGCGCTGAGCGGCGCGCCGGTGGTGGTGGTGGGCTGCGCGCAGACGCTGCGGCGGATCCTGGCGCTAAATATCACACCGCGGGCGGAGCTGCGCATTATTGACCATCCCGCCGAGGCGAGTTTTTCCCCAGCGACTATCAACGTCATTGATGAACCACTCAGCGACCCGCAGGGGCTGCGCCCTGGCGAAGTCCAGGCCCAGGCAGGGGATCTCGCGTTTCGCTGCATCAGGCGGGCGACCGCGCTGGCGCTGGAGGGCGCGGTCGCGGCCATCGCTACCGCGCCGCTGAATAAAGAGGCGCTGCATCTCGCCGGGCACGCTTATCCCGGCCATACCGAACTGCTGGCCCACCTGACGCAGACTACCGATTACGCCATGGTGCTCTATACCGAGAAGCTTAAGGTTATCCATATCACTACCCATATTTCCTTGCGCCAGTTCCTCGATACCCTCAACCAGCCGCGGATTGAGACGGTGATTGGCGTGGCGGATCGCTTTCTGCGCCGCGTCGGCTATCCACGTCCGCGCATCGCGGTCGCCGGCGTTAACCCGCACGCCGGGGAGAACGGCCTGTTTGGCGATGAAGAGATCCGTATCGTCGCCCCGGCGGTCGCCGCCATGCGGGCGAAGGGAGTGGAGGTGACCGGCCCCTGTCCGCCGGACACGGTGTTTATGCAGTGTCACGAAGGCATGTACGACATGGTGGTGGCGATGTATCACGACCAGGGGCATATTCCGCTGAAGCTGCTGGGATTCTATGACGGGGTGAATATTACCGCCGGGCTGCCGTTTATCCGTACCTCCGCGGATCATGGCACTGCGTTTGATATTGCCTGGACAGGTAAAGCGAAGTCTGAGAGCATGGCAACCTCCATTGAGCTCGCCATGCACATCGCGCAGGAA
BLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq 3 sequences; 3,332 total letters Query= IDP96938.105 Length=1389 Score E Sequences producing significant alignments: (Bits) Value 2_12A_A9_IDP96938.105_T7term.seq 1842 0.0 0_wt 1775 0.0 1_09A_A9_IDP96938.105_T7S.seq 1674 0.0 F P S R N N F V * L * E G D I H M H H H H H H S S G V D L Query_1 58 TTCCCCTCTAGAAATAATTTT--GTTTAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATTCTTCTGGTGTAGATCTGT 145 1 11 .........N...........TT................................................------------------- 81 W S H P Q F E K G T E N L Y F Q S N A V S K M I A V T M G Query_1 146 GGTCTCATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGTGT-CTAAGATGATTGCAGTG-ACAATGGGCGA 233 2 1126 ...N..A...TN..NA............G.......N... 1087 0 1 ....-.................-........... 32 1 82 -----------------------------...........................A...-.................-........... 140 D P A G I G P E I I I K S L A E G A L S G A P V V V V G C A Query_1 234 CCCGGCAGGGATTGGTCCGGAGATTATTAT-CAAATCCCTCGCCGAGGGGGCGCTGAGCGGCGCGCCGGTGGTGGTGGTGGG-CTGCGCG 321 2 1086 ..-......-......N..-....-.....T..........N....A..........NN.....A...........A..A..A....... 1001 2 58 ............. 70 2 57 ............ 68 0 33 ..............................-...................................................-....... 120 1 141 ..............................-...................................................-....... 228 Q T L R R I L A L N I T P R A E L R I I D H P A E A S F S Query_1 322 CAGACGCTGCGGCGGATCCTGGCGCTAAATATCACACCGCGGGCGGAGCTGCGCA-TTATTGACCATCCCGCCGAGGCGAGTTTTTCCCC 410 2 1000 ..............-....N...................................A.................................. 912 0 121 .......................................................-.................................. 209 1 229 .......................................................-.................................. 317 1 801 ............ 790 P A T I N V I D E P L S D P Q G L R P G E V Q A Q A G D L A Query_1 411 AGCGACTATCAACGTCATTGATGAACCACTCAGCGACCCGCAGGGGCTGCGCCCTGGCGAAGTCCAGGCCCAGGCAGGGGATCTCGCGTT 500 2 911 .......................................................................................... 822 0 210 .......................................................................................... 299 1 318 .......................................................................................... 407 F R C I R R A T A L A L E G A V A A I A T A P L N K E A L H Query_1 501 TCGCTGCATCAGGCGGGCGACCGCGCTGGCGCTGGAGGGCGCGGTCGCGGCCATCGCTACCGCGCCGCTGAATAAAGAGGCGCTGCATCT 590 2 821 ................................................A......................................... 732 0 300 .......................................................................................... 389 1 408 .......................................................................................... 497 L A G H A Y P G H T E L L A H L T Q T T D Y A M V L Y T E K Query_1 591 CGCCGGGCACGCTTATCCCGGCCATACCGAACTGCTGGCCCACCTGACGCAGACTACCGATTACGCCATGGTGCTCTATACCGAGAAGCT 680 2 731 .......................................................................................... 642 0 390 .......................................................................................... 479 1 498 .......................................................................................... 587 L K V I H I T T H I S L R Q F L D T L N Q P R I E T V I G V Query_1 681 TAAGGTTATCCATATCACTACCCATA-TTTCCTTGCGCCAGTTCCTCGATACCCTCAACCAGCCGCGGATTGAGACGGTGA-TTGGCGTG 768 2 641 ..........................-......................................................-........ 554 0 480 ..........................-......................................................-........ 567 1 588 ..........................T............N......................................C..T........ 677 A D R F L R R V G Y P R P R I A V A G V N P H A G E N G L F Query_1 769 GCGGATCGCTTTCTGCGCCGCGTCGGCTATCCACGTCCGCGCATCGCGGTCGCCGGCGTTAACCCGCACGCCGGGGAGAACGGCCTGTTT 858 2 553 .......................................................................................... 464 0 568 .......................................................................................... 657 1 678 .......................................................................................... 767 G D E E I R I V A P A V A A M R A K G V E V T G P C P P D T Query_1 859 GGCGATGAAGAGATCCGTATCGTCGCCCCGGCGGTCGCCGCCATGCGGGCGAAGGGAGTGGAGGTGACCGGCCCCTGTCCGCCGGACACG 948 2 463 .......................................................................................... 374 0 658 .......................................................................................... 747 1 768 ..N........................T.............................................................. 857 V F M Q C H E G M Y D M V V A M Y H D Q G H I P L K L L G F Query_1 949 GTGTTTATGCAGTGTCACGAAGGCATGTACGACATGGTGGTGGCGATGTATCACGACCAGGGGCATATTCCGCTGAAGCTGCTGGGATTC 1038 2 373 .......................................................................................... 284 0 748 .......................................................................................... 837 1 858 ...........N............................N.C................-.........-.......N............ 945 Y D G V N I T A G L P F I R T S A D H G T A F D I A W T G K Query_1 1039 TATGACGGGGTGAATATTACCGCCGGGCTGCCGTTTATCCGTACCTCCGCGGATCATGGCACTGCGTTTGATATTGCCTGGACAGGTAAA 1128 2 283 .......................................................................................... 194 0 838 .......................................................................................... 927 1 946 ......-.........-.............-...............-..........-........-......-..G.......-..... 1027 A K S E S M A T S I E L A M H I A Q E * H W K W I T D P N Query_1 1129 G-CGAAGTCTGAGAGCATGGCAACCTCCATTGAGCTCGCCATGCACATCGCGCAGGAATAACATTGGAAGTGGATAACGGATC-CGAATT 1216 2 193 .-.................................................................................-...... 106 0 928 .-........................................................ 984 1 1028 .C..........C...N.....N......C..GNN.....-N....CATN..N.........-............N.....A.T....N. 1115 S S S V D K L A A A L E H H H H H H * D P A A N K A R K E Query_1 1217 CGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGcaccaccaccacc-accacTGAGATCCGGCTGCTAAC-AAAGCCCG-AAAGGAA 1302 2 105 ................................................A.......................A........A....... 17 1 1116 -N..... 1121 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Effective search space used: 4508932 Database: /mnt/ydrive- ccfa/ExperimentalData/Cloning/Sequencing/UofC.BLAST.ByCloneID/input/ allseq Posted date: Apr 4, 2018 11:11 AM Number of letters in database: 3,332 Number of sequences in database: 3 Matrix: blastn matrix 2 -3 Gap Penalties: Existence: 5, Extension: 2