Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451 3,845,705 +G intergenic (‑72/+26) PP_3394 ← / ← PP_3395 putative 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/Transcriptional regulator, LysR family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154513,845,7031.G96.8% 206.1 / ‑2.3 62intergenic (‑70/+28)PP_3394/PP_3395putative 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/Transcriptional regulator, LysR family
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base G (34/26);  total (34/28)
Fisher's exact test for biased strand distribution p-value = 2.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCACGGTTACTTGCAGGTCCGGGTACGTGAGGGCGTGAGCCACCACTTCGCGGGCATCGGCCATCTGCGGCAGCAAGCGTGCCGGCACGAACGACGCGACTTCCATATGGCGCACGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑GGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCTTGCGGGAACACCGCCAGGCCAAGGCCAAACTGCACCAGACGGTATGCCGCGTCGAACGACGACACGTACGAACGAAAACGCAGCGCC  >  AE015451/3845403‑3845995
                                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                     
cAGCACGGTTACTTGCAGGTCCGGGTACGTGAGGGCGTGAGCCACCACTTCGCGGGCATCGGCCATCTGCGGCAGCAAGCGTGCCGGCACGAACGACGCGACTTCCATATGGCGCACGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTc‑gc                                                                                                                                                                                                                                                                                                        >  1:150785/1‑301 (MQ=255)
   cACGGTTACTTGCAGGTCCGGGTACGTGAGGGCGTGAGCCACCACTTCGCGGGCATCGGCCATCTGCGGCAGCAAGCGTGCCGGCACGAACGACGCGACTTCCATATGGCGCACGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑ggg                                                                                                                                                                                                                                                                                                    >  3:353018/1‑300 (MQ=255)
              gagttcagacgtgtgctcttccgatctccaccaCTTCGCGGGCATCGGCCATCTGCGGCAGCAAGCGTGCCGGCACGAACGACGCGACTTCCATATGGCGCACGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCg                                                                                                                                                                                                                                                                                         <  4:568398/274‑1 (MQ=255)
                aGGTCCGGGTACGTGAGGGCGTGAGCCACCACTTCGCGGGCATCGGCCATCTGCGGCAGCAAGCGTGCCGGCACGAACGACGCGACTTCCATATGGCGCACGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTc                                                                                                                                                                                                                                                                                       >  3:322907/1‑301 (MQ=255)
                 ggTCCGGGTACGTGAGGGCGTGAGCCACCACTTCGCGGGCATCGGCCATCTGCGGCAGCAAGCGTGCCGGCACGAACGACGCGACTTCCATATGGCGCACGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCa                                                                                                                                                                                                                                                                                      <  3:175227/301‑1 (MQ=255)
                              gAGGGCGTGAGCCACCACTTCGCGGGCATCGGCCATCTGCGGCAGCAAGCGTGCCGGCACGAACGACGCGACTTCCATATGGCGCACGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATc                                                                                                                                                                                                                                                                         >  3:211775/1‑301 (MQ=255)
                                         ccaccaCTTCGCGGGCATCGGCCATCTGCGGCAGCAAGCGTGCCGGCACGAACGACGCGACTTCCATATGGCGCACGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCgagatcggaagcgcgtcgtgtagggaaa                                                                                                                                                                                                                                                              >  2:568255/1‑274 (MQ=255)
                                                         aTCGGCCATCTGCGGCAGCAAGCGTGCCGGCACGAACGACGCGACTTCCATATGGCGCACGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCCCCCGTCCAGGCCGGTGccc                                                                                                                                                                                                                                              >  1:331247/1‑299 (MQ=255)
                                                              ccATCTGCGGCAGCAAGCGTGCCGGCACGAACGACGCGACTTCCATATGGCGCACGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACgcagc                                                                                                                                                                                                                                         >  3:479154/1‑301 (MQ=255)
                                                              ccATCTGCGGCAGCAAGCGTGCCGGCACGAACGACGCGACTTCCATATGGCGCACGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGAATTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGATGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGACCGAGCCCTGCCAggaaggggcaggcagc                                                                                                                                                                                                                                         >  1:506717/1‑287 (MQ=255)
                                                               cATCTGCGGCAGCAAGCGTGCCGGCACGAACGACGCGACTTCCATATGGCGCACGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACgcagca                                                                                                                                                                                                                                        >  4:737055/1‑301 (MQ=255)
                                                                      ggCAGCAAGCGTGCCGGCACGAACGACGCGACTTCCATATGGCGCACGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAggtg                                                                                                                                                                                                                                 >  4:364231/1‑301 (MQ=255)
                                                                           taccgatgtcggaacgaacgaCGCGACTTCCAAATGGCGAACGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCaa                                                                                                                                                                                                                            <  3:233819/288‑1 (MQ=255)
                                                                                       cACGAACGACGCGACTTCCATATGGCGCACGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccgag                                                                                                                                                                                                                <  1:400684/301‑1 (MQ=255)
                                                                                                  cgACTTCCATATGGCGCACGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCgag                                                                                                                                                                                                     <  3:221789/301‑1 (MQ=255)
                                                                                                   gACTTCCATATGGCGCACGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCgaga                                                                                                                                                                                                    >  3:28802/1‑301 (MQ=255)
                                                                                                     cTTCCATATGGCGCACGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGc                                                                                                                                                                                                  <  1:479140/301‑1 (MQ=255)
                                                                                                         catgtGGCGAACGGCGGGGCTGTAGGCGGGGTCGATCCAGCGGCGTTGGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCgtgt                                                                                                                                                                                              <  2:364158/297‑1 (MQ=255)
                                                                                                                       cGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCAGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGCGCGCGTGTGCCGGTCgcgcctt                                                                                                                                                                                >  1:428940/1‑298 (MQ=255)
                                                                                                                           gcCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGCGAGCGTGTGCCGGTCGCGCATGCAgg                                                                                                                                                                            >  2:679127/1‑300 (MQ=255)
                                                                                                                             cGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATg                                                                                                                                                                          <  3:150791/301‑1 (MQ=255)
                                                                                                                                     gcgTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTc                                                                                                                                                                  <  3:331255/301‑1 (MQ=255)
                                                                                                                                      cgTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCa                                                                                                                                                                 >  4:537000/1‑301 (MQ=255)
                                                                                                                                                  ggggtgtGGCTGCTGTCGGTATGATCACTTGCAGGCGCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCGGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAg                                                                                                                                                     <  2:736841/295‑1 (MQ=255)
                                                                                                                                                        tGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGCGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTTCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAgtcgtcc                                                                                                                                               >  2:67068/1‑300 (MQ=255)
                                                                                                                                                            tgctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGACTTCACCAAgcgcacaaccggaagtcc                                                                                                                                           >  2:377297/1‑287 (MQ=255)
                                                                                                                                                                gTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCCAGAGCGTGTGCCGGTCGCGCATGCAGCTGACGAATTTCCCCAGCCCCCACTCCTCCGTCAgcgc                                                                                                                                       >  2:229438/1‑301 (MQ=255)
                                                                                                                                                                  cGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCaa                                                                                                                                     >  4:527214/1‑301 (MQ=255)
                                                                                                                                                                     cATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGa                                                                                                                                  >  4:68990/1‑301 (MQ=255)
                                                                                                                                                                            aCTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGGCAGCGCAATGACCCGCAg                                                                                                                           >  3:540335/1‑301 (MQ=255)
                                                                                                                                                                             cTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAgg                                                                                                                          >  4:695212/1‑301 (MQ=255)
                                                                                                                                                                                  aGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGaa                                                                                                                     >  3:625128/1‑301 (MQ=255)
                                                                                                                                                                                       ctGCACGCCATCGCGAAGGCCTACTTCATTGATTGTTATGTTTATCAGCGCGGTCTCTCTGTCTGATGCGTTGCGCAAGACTAAGAGCTGGATTACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAAcg                                                                                                                <  1:353008/301‑1 (MQ=255)
                                                                                                                                                                                               cATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGAAGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACCACGACGCATAg                                                                                                        >  1:317405/1‑301 (MQ=255)
                                                                                                                                                                                                 ccgcgCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCg                                                                                                      <  1:540321/300‑1 (MQ=255)
                                                                                                                                                                                                   gcgcAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCggg                                                                                                    <  1:28801/301‑1 (MQ=255)
                                                                                                                                                                                                            gACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGTTGCACGCCTCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGATCGTGTGCCGGTCGCGCATGCCAATGACGAATTCCCCAAGCGCCCAGCCGTCCGTCCGCGCAATGACCCTCCGGTCGAACAAcgcccactcacggcacaccgccc                                                                                           >  4:667942/1‑282 (MQ=255)
                                                                                                                                                                                                              cTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCCTGCAGATGACGCATTCACCAAGCGCCCATTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCCTAGCGGGacaccggcccc                                                                                         >  2:463495/1‑294 (MQ=255)
                                                                                                                                                                                                                 cATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCAACAAGCGCCCAGTCCTCAGTCAGCGCCATGACCCGCAGGTCGAACAACGCCGCACACCGGGaccgcgcgcctggc                                                                                      >  2:9640/1‑289 (MQ=255)
                                                                                                                                                                                                                   ttgattgTTATTGTTGTCATCGCGGGTTCTCGGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCTTGCgg                                                                                    <  1:322899/301‑1 (MQ=255)
                                                                                                                                                                                                                          ttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGCGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCTTGCGGGAACAcc                                                                             <  4:67083/301‑1 (MQ=255)
                                                                                                                                                                                                                           tattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCTTGCGGGAACAccc                                                                            >  4:289711/1‑300 (MQ=255)
                                                                                                                                                                                                                             ttgttgTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCTTGCGGGAACACCGcc                                                                          <  4:377373/301‑1 (MQ=255)
                                                                                                                                                                                                                             ccgatctCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCTTGCGGGAACACCGcc                                                                          <  3:612702/295‑1 (MQ=255)
                                                                                                                                                                                                                                   ccATCGCGGTCTCTCTGTCTGAAGCTTTGCTCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCTTGCGGGAACACCGCCaggcca                                                                    <  3:428951/300‑1 (MQ=255)
                                                                                                                                                                                                                                    cATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCTTGCGGGAACACCGCCagatcgg                                                                   >  1:612688/1‑296 (MQ=255)
                                                                                                                                                                                                                                         cgGTCTCTCTGTCTAAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCTTGCGGGAACACCGCCaggccaaggcca                                                              >  4:64500/1‑301 (MQ=255)
                                                                                                                                                                                                                                                           cGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCTTGCGGGAACACCGCCAGGCCAAGGCCAAACTGCACCAGACGGTAt                                            <  3:317413/301‑1 (MQ=255)
                                                                                                                                                                                                                                                            gTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCTTGCGGGAACACCGCCAGGCCAAGGCCAAACTGCACCAGACGGTATg                                           >  3:277128/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                 gcacgacgaaGAGCTGGATGACGGGCAGGAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGAGCAGACCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCTTGCGGGAACACCGCCAGGCCAAGGCCAAACTGCACCAGACGGTATGCcgcg                                      <  2:667766/293‑1 (MQ=255)
                                                                                                                                                                                                                                                                       atAAGGGCTGAATGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑GGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCTTGCGGGAACACCGCCAGGCCAAGGCCAAACTGCACCAGACGGTATGCCGCGTCGAac                                <  1:211773/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                         aagggcgtgATGACGGGCAGAAAAGACGTGGTTCTGC‑GGC‑TGATCCACCGTCAGCCCTGATGGATCATCCCGCGCCCGGCAAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCTTGCGGGAACACCGCCAGGCCAAGGCCAAACTGCACCAGACGGTATGCCGCGTCGAacga                              <  2:64485/293‑1 (MQ=255)
                                                                                                                                                                                                                                                                          ggagCTGGAGGACGGGCAGAAAGGAGGGGTTCG‑GC‑GGCGTGATCCACCGTCAGCCAGGAGGGAGAGGCCCGCGCCGGGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCTTGCGGGAACACCGCCAGGCCAAGGCCAAACTGCACCAGACGGTATGCCGCGTCGAacgac                             <  2:536867/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                          agagCGGGATGCCGGGCAGAAAAGACGTGTTCC‑GCGGGC‑TGATCCATCGGCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCTTGCGGGAACACCGCCAGGCCAAGGCCAAACTGCACCAGACGGTATGCCGCGTCGAacgac                             <  1:625113/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                          acgtgtgctcctccgatctaaaaGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCTTGCGGGAACACCGCCAGGCCAAGGCCAAACTGCACCAGACGGTATGCCGCGTCGAacgac                             <  3:464956/282‑1 (MQ=255)
                                                                                                                                                                                                                                                                             gtgggatggaggcgagaaagcaCGTGTTTC‑GCGGGC‑TGAGCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCTTGCGGGAACACCGCCAGGCCAAGGCCAAACTGCACCAGACGGTATGCCGCGTCGAACgacgac                          <  2:527081/280‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  aTGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCTTGCGGGAACACCGCCAGGCCAAGGCCAAACTGCACCAGACGGCATGCCGCGTCGACCGACGACACgca                     >  4:644047/1‑299 (MQ=255)
                                                                                                                                                                                                                                                                                  aTGACGGGCAGAAAAGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGACGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCTTGCGGGAACACCGCCAGGCCAAAGCCAAACTGCACCAGACGGTATGCCGCGTCGAACGACGACACGTa                     <  3:506732/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                       ccgatctaaaGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCTTGCGGGAACACCGCCAGGCCAAGGCCAAACTGCACCAGACGGTATGCCGCGTCGAACGACGACACGTacgaac                <  4:315387/294‑1 (MQ=255)
                                                                                                                                                                                                                                                                                             aaaaGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCTTGCGGGAACACCGCCAGGCCAAGGCCAAACTGCACCAGACGGTATGCCGCGTCGAacgacagatcggaagcgcgtcgtg          >  1:464943/1‑282 (MQ=255)
                                                                                                                                                                                                                                                                                             aaaaGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCTTGCGGGAACACCGCCAGGCCAAGGCCAAACTGCACCAGACGGTATGCCGCGTCGAACGACGTCACgcaacaaccacaaa          >  2:401161/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                              aaaGACGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCTTGCGGGAACACCGCCAGGCCAAGGCCAAACTGCACCAGACGGTATGCCGCGTCGAACGACGACACGTaccaacagatcgg         >  2:315325/1‑294 (MQ=255)
                                                                                                                                                                                                                                                                                                   cGTGTTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCTTGCGGGAACACCGCCAGGCCAAGGCCACACTGCACCAGACGGTATGCCGCGTCGAACGCCGACACGTACGACCGAAAACGCAgcc    >  2:212811/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                     tgtTTC‑GCGGGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCTTGCGGGAACACCGCCAGGCCAAGGCCAAACTGCACCAGACGGTATGCCGCGTCGAACGACGACACGTACGAACGAAAACGCAGCGcc  >  3:578841/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                     
CAGCACGGTTACTTGCAGGTCCGGGTACGTGAGGGCGTGAGCCACCACTTCGCGGGCATCGGCCATCTGCGGCAGCAAGCGTGCCGGCACGAACGACGCGACTTCCATATGGCGCACGCCGGCGCCGTAAGCGGCGTCGATCCAGCGGCGTTTGGCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑GGC‑TGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCCCAGTCGTCAGTCAGCGCAATGACCCGCAGGTCGAACAACGCCGCATAGCGGGACACGGCCTCTTGCGGGAACACCGCCAGGCCAAGGCCAAACTGCACCAGACGGTATGCCGCGTCGAACGACGACACGTACGAACGAAAACGCAGCGCC  >  AE015451/3845403‑3845995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑