Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451	4,025,384	T→C	E105E (GAA→GAG)	emrA ←	multidrug efflux transport system ‑ membrane fusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	4,025,384	0	T	C	100.0%	339.4 / NA	90	E105E (GAA→GAG)	emrA	multidrug efflux transport system ‑ membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (50/40);  total (50/40)

AGCTGCAGCCATGACATCGGGGTGCGAGGACACCACGGTGTCGTCGACCAGCGCGCTGCTGGTGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCTTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAACAGCAGGCCAAGCAGCCAAGCCTTGCGCTTGCGCGACGGCTCAGGCGCGACGGAGGGAGTC  >  AE015451/4025090‑4025675                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                    aGCTGCAGCCATGACATCGGGGTGCGAGGACACCACGGTGTCGTCGACCAGCGCGCTGCTGGTGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTcggcg                                                                                                                                                                                                                                                                                               >  3:192178/1‑301 (MQ=255)    tGCAGCCATGACATCGGGGTGCGAGGACACCACGGTGTCGTCGACCAGCGCGCTGCTGGTGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGAc                                                                                                                                                                                                                                                                                            >  3:548270/1‑301 (MQ=255)    ggCAGCCATGACATCGGGGTGCGAGGACACCACGGTGTCGTCGACCAGCGCGCTGCTGGTGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTG‑AGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACt                                                                                                                                                                                                                                                                                           <  1:161217/300‑1 (MQ=255)         ccATGACATCGGGGTGCGAGGACACCACGGTGTCGTCGACCAGCGCGCTGCTGGTGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCaa                                                                                                                                                                                                                                                                                       >  4:719966/1‑301 (MQ=255)          cATGACATCGGGGTGCGAGGACACCACGGTGTCGTCGACCAGCGCGCTGCTGGTGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAg                                                                                                                                                                                                                                                                                      <  1:192176/301‑1 (MQ=255)          cATGACATCGGGGTGCGAGAACACCACGGTGTCGTCGACCAGCGCGCTGCTGGTGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAg                                                                                                                                                                                                                                                                                      <  1:625719/301‑1 (MQ=255)             gACATCGGGGTGCGAGGACACCACGGTGTCGTCGACCAGCGCGCTGCTGGTGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGcc                                                                                                                                                                                                                                                                                   >  3:508241/1‑301 (MQ=255)                       tGCGAGGACACCACGGTGTCGTCGACCAGCGCGCTGCTGGTGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCCCTTCGCTGt                                                                                                                                                                                                                                                                         >  1:450911/1‑301 (MQ=255)                            ggACACCACGGTGTCGTCGACCAGCGCGCTGCTGGTGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGcc                                                                                                                                                                                                                                                                    <  3:450923/301‑1 (MQ=255)                                accacGGTGTCGTCGACCAGCGCGCTGCTGGTGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTTCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGCCGGCCgggg                                                                                                                                                                                                                                                                >  2:125510/1‑300 (MQ=255)                                  cacGGTGTCGTCGACCAGCGCGCTGCTGGTGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCg                                                                                                                                                                                                                                                              >  3:505395/1‑301 (MQ=255)                                     ggTGTCGTCGACCAGCGCGCTGCTGGTGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAAt                                                                                                                                                                                                                                                           <  3:594147/301‑1 (MQ=255)                                           gtcgACCAGCGCGCTGCTGGTGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTgcagc                                                                                                                                                                                                                                                     >  1:115422/1‑301 (MQ=255)                                                 cAGCGCGCTGCTGGTGCTGGGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATAcc                                                                                                                                                                                                                                               <  4:605391/301‑1 (MQ=255)                                                    cgcgcTGCTGGTGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTgg                                                                                                                                                                                                                                            <  3:295386/301‑1 (MQ=255)                                                      ggctgctGGTGCTGAGCGGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGcc                                                                                                                                                                                                                                          <  1:175793/300‑1 (MQ=255)                                                      cgcTGCTGGTGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCCGCCATACCTGGcc                                                                                                                                                                                                                                          >  2:87241/1‑301 (MQ=255)                                                         tgctgGTGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGc                                                                                                                                                                                                                                       >  3:335351/1‑301 (MQ=255)                                                            tgGTGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATg                                                                                                                                                                                                                                    >  3:143829/1‑301 (MQ=255)                                                             ggggcggggCTGTGGCCGGGCGCTGTGGACGGCAGCCTGGGCAACGCTCAGGTCTTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATgg                                                                                                                                                                                                                                   <  2:680572/293‑1 (MQ=255)                                                                 gtGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCa                                                                                                                                                                                                                               <  1:548256/300‑1 (MQ=255)                                                                                     gTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCg                                                                                                                                                                                                           >  4:191533/1‑301 (MQ=255)                                                                                             cAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGt                                                                                                                                                                                                   <  4:124605/301‑1 (MQ=255)                                                                                               gCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTaa                                                                                                                                                                                                 >  3:567038/1‑301 (MQ=255)                                                                                                ccTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAAc                                                                                                                                                                                                >  4:281334/1‑301 (MQ=255)                                                                                                 cTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACg                                                                                                                                                                                               >  4:82347/1‑301 (MQ=255)                                                                                                    ggCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTg                                                                                                                                                                                            >  4:11009/1‑301 (MQ=255)                                                                                                          cctcgtgtcgtaACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGTTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCg                                                                                                                                                                                      <  1:335343/295‑1 (MQ=255)                                                                                                                    tcACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGAATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCgggg                                                                                                                                                                            <  4:97225/301‑1 (MQ=255)                                                                                                                        cggCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGTCCCAGTCGCCTCCCTCGGCCCCGATGCTGGTAACGGTTCCatgcacctgcggggggcg                                                                                                                                                                        >  2:347486/2‑284 (MQ=255)                                                                                                                         ggCGTGGGACAGTGCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGGGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGAtt                                                                                                                                                                       <  1:548193/301‑1 (MQ=255)                                                                                                                              gggACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTccac                                                                                                                                                                  <  3:484754/301‑1 (MQ=255)                                                                                                                                 aCAGTTCTTCTGCGGCAATCGAACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCcaccac                                                                                                                                                               <  4:540726/301‑1 (MQ=255)                                                                                                                                       cttctGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGcc                                                                                                                                                         <  1:106386/301‑1 (MQ=255)                                                                                                                                       cttctGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCAACCAGCGGGGTGATTTCCACCACGTTGcc                                                                                                                                                         <  3:115425/301‑1 (MQ=255)                                                                                                                                          ctgggccAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGtt                                                                                                                                                      <  2:719773/295‑1 (MQ=255)                                                                                                                                           tGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTGGCGCCTTGCGCAGTTCGGCTTTGCGCGTTTCCATTTGAGCCTTCAGCGAGTCGACGTAGCTGTAAAGCCCGCGCA‑CCTACCGCACGCAGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCCATACCGGGACGGCATGGACCAGGGCGCCGTCATCGGCCCCGGTGCTGGTAAAGGTTGCAGCAACAAGCGGGGTGGTTTCCACCACGTTGccagtg                                                                                                                                                     >  1:355319/1‑297 (MQ=255)                                                                                                                                                 aaTCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATTCTGGTAACGGGGCCAGCGACCAGCGGGGTGATTTCCACCACGTTTCCGTTGCCGTAg                                                                                                                                               >  2:57384/1‑301 (MQ=255)                                                                                                                                                   cggaaccgcgtgcggccagcAACGTGGGCCGGTTGAAGTCCTGTTTCGGCTTGGGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTGGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGc                                                                                                                                             <  2:660856/287‑1 (MQ=255)                                                                                                                                                     gCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCAt                                                                                                                                           >  1:324641/1‑301 (MQ=255)                                                                                                                                                     gCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCAt                                                                                                                                           <  4:125537/301‑1 (MQ=255)                                                                                                                                                     gCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCAt                                                                                                                                           <  1:508227/301‑1 (MQ=255)                                                                                                                                                     gCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGACGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACCTAGGCAt                                                                                                                                           >  1:324647/1‑301 (MQ=255)                                                                                                                                                     ccACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCAt                                                                                                                                           <  3:544188/300‑1 (MQ=255)                                                                                                                                                     ccACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCAt                                                                                                                                           <  2:281282/300‑1 (MQ=255)                                                                                                                                                             gTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTATAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCGGGTAACGGTGCCAGCGACCCGCGGGGTGACTTCCACCCCGTTGCCTGTGAAGTAGGCATCGTCGGTg                                                                                                                                   >  2:202420/1‑301 (MQ=255)                                                                                                                                                                agccagcACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATACTGGTAACGGTGCCCGCGACCAGCGGGGTACTTTCCACCccgttgccgttggccgaggcagtcgtcgttgtt                                                                                                                                >  2:111586/1‑280 (MQ=255)                                                                                                                                                                 gccagcACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTTCttt                                                                                                                               >  1:309719/1‑301 (MQ=255)                                                                                                                                                                 gccagcACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATACTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCttt                                                                                                                               <  4:111610/301‑1 (MQ=255)                                                                                                                                                                  ccagcACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCtttt                                                                                                                              >  2:118055/1‑300 (MQ=255)                                                                                                                                                                   cagcACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCTCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCg                                                                                                                             <  1:505380/301‑1 (MQ=255)                                                                                                                                                                     gcACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTg                                                                                                                           <  1:567023/301‑1 (MQ=255)                                                                                                                                                                                cGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGa                                                                                                                >  3:499969/1‑301 (MQ=255)                                                                                                                                                                                  gtggAAGTCCCGTTGCGCCTTGCGCAGGTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACa                                                                                                              <  2:191499/298‑1 (MQ=255)                                                                                                                                                                                     cttccgatcttGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCG‑GTACCCTTCCTCCCTCTTCTTACCAGCTTGGCCTGTGTGTTCTGCATGGTGACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATg                                                                                                           <  2:624573/292‑1 (MQ=255)                                                                                                                                                                                               tGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATgagatcggaag                                                                                                 >  4:624734/1‑291 (MQ=255)                                                                                                                                                                                                   ccTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGTCGTCTGCATCGTCGGTGCTTTGGTGCACACCCCCCACAATGTTGGACCAGGcccc                                                                                             >  2:165980/1‑300 (MQ=255)                                                                                                                                                                                                     ttctgcAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAgg                                                                                           <  3:305736/297‑1 (MQ=255)                                                                                                                                                                                                       gcgcAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTg                                                                                         <  4:87260/301‑1 (MQ=255)                                                                                                                                                                                                         gcAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAggagaa                                                                                       >  1:305728/1‑297 (MQ=255)                                                                                                                                                                                                          cccctacccgacgctcttccgtacttgggCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCg                                                                                      <  2:668608/273‑1 (MQ=255)                                                                                                                                                                                                           aGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGa                                                                                     >  3:443948/1‑301 (MQ=255)                                                                                                                                                                                                                  cTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACACTGCTTTACCAGGCCCAGGTTCCGGCCCCGGc                                                                              >  2:437301/1‑301 (MQ=255)                                                                                                                                                                                                                    tGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCaa                                                                            >  4:174337/1‑301 (MQ=255)                                                                                                                                                                                                                     ggCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAg                                                                           <  4:118079/301‑1 (MQ=255)                                                                                                                                                                                                                         cgTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATc                                                                       >  3:409086/1‑301 (MQ=255)                                                                                                                                                                                                                               cAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCagcagc                                                                 >  3:314257/1‑301 (MQ=255)                                                                                                                                                                                                                                  ttGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAAc                                                              <  3:309727/301‑1 (MQ=255)                                                                                                                                                                                                                                   tGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGCCAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGCATCAGCAGCAAca                                                             >  4:482965/1‑301 (MQ=255)                                                                                                                                                                                                                                   tGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGagatcggaagagcacacgtctgaac                                                             >  4:668784/1‑277 (MQ=255)                                                                                                                                                                                                                                   tGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCCGCAAca                                                             >  3:198243/1‑301 (MQ=255)                                                                                                                                                                                                                                       ccTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAAcagcag                                                         <  4:347554/301‑1 (MQ=255)                                                                                                                                                                                                                                        cTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTTCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAACAGCAgg                                                        >  3:347873/1‑301 (MQ=255)                                                                                                                                                                                                                                        cTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAACAGCAgg                                                        >  1:259218/1‑301 (MQ=255)                                                                                                                                                                                                                                         ttCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCCGCAGCAACAGCAggg                                                       >  3:215545/1‑300 (MQ=255)                                                                                                                                                                                                                                           cAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAACAGCAGGCCa                                                     >  3:486096/1‑301 (MQ=255)                                                                                                                                                                                                                                             gCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCGAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCACTACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGTGTGATTTCAACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAACAGCAGGCCAag                                                   <  3:355329/301‑1 (MQ=255)                                                                                                                                                                                                                                                   ggACGTTGCGGTAAAGCCCGCGAA‑CCTGCCGAACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAACAGCAGGCCAAGCAGCCa                                             <  1:409076/300‑1 (MQ=255)                                                                                                                                                                                                                                                        ttGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAACAGCAGGCCAAGCAGCCAAGCct                                        <  3:491841/301‑1 (MQ=255)                                                                                                                                                                                                                                                        ttGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAACAGCAGGCCAAGCAGCCAAGCct                                        <  4:57396/301‑1 (MQ=255)                                                                                                                                                                                                                                                                  gCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAACAGCAGGCCAAGCAGCCAAGCcttgcgcttgcg                              >  3:422522/1‑301 (MQ=255)                                                                                                                                                                                                                                                                    ccGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAACAGCAGGCCAAGCAGCCAAGCCTTGCGCTTGcgcg                            >  3:290509/1‑301 (MQ=255)                                                                                                                                                                                                                                                                        gcA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAACAGCAGGCCAAGCAGCCAAGCCTTGCGCTTGCGCGACgg                        >  3:412767/1‑301 (MQ=255)                                                                                                                                                                                                                                                                        gcA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAACAGCAGGCCAAGCAGCCAAGCCTTGCGCTTGCGCGACgg                        >  3:576865/1‑301 (MQ=255)                                                                                                                                                                                                                                                                         cA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAACAGCAGGCCAAGCAGCCAAGCCTTGCGCTTGCGCGACGGc                       >  3:574356/1‑301 (MQ=255)                                                                                                                                                                                                                                                                            ccTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAACAGCAGGCCAAGCAGCCAAGCCTTGCGCTTGCGGGACGGCTc                     >  4:374204/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                 cGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAACAGCAGGCCAAGCAGCCAAGCCTTGCGCTTGCGCGACGGCTCAGgcg                >  4:434662/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                     cGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAACAGCAGGCCAAGCAGCCAAGCCTTGCGCTTGCGCGACGGCTCAGGCGCGag            >  4:561229/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      gCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGCTCAGCAGCAACAGCCGGCCAAGCAGCCAAGCCTTCAGCTTGCGCGACGGCTCAGGCGCGAcc           >  2:97351/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                               cAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAACAGCAGGCCAAGCAGCCAAGCCTTGCGCTTGCGCGACGGCTCAGGCGCGACGGAGGGAGTc  <  1:443936/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                    AGCTGCAGCCATGACATCGGGGTGCGAGGACACCACGGTGTCGTCGACCAGCGCGCTGCTGGTGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCA‑CCTGCCGCACGCTGCGTGCCAGCTTGGCTTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAACAGCAGGCCAAGCAGCCAAGCCTTGCGCTTGCGCGACGGCTCAGGCGCGACGGAGGGAGTC  >  AE015451/4025090‑4025675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑