| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AE015451 | 1,499,480 | 2 bp→AC | intergenic (+57/‑106) | trpS → / → zapE | Tryptophan‑‑tRNA ligase/putative enzyme with nucleoside triphosphate hydrolase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 1,499,480 | 0 | C | A | 98.6% | 257.3 / NA | 73 | intergenic (+57/‑107) | trpS/zapE | Tryptophan‑‑tRNA ligase/putative enzyme with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (35/37); total (36/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | AE015451 | 1,499,481 | 0 | A | C | 100.0% | 260.7 / NA | 73 | intergenic (+58/‑106) | trpS/zapE | Tryptophan‑‑tRNA ligase/putative enzyme with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (36/37); total (36/37) | |||||||||||
GCGAGCAACTGCTGTTGTCGCGCAGCTTCGCCGACGGCAAGAGTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCACG‑C‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAACAAGCCGGGCATGTTCGGCAAGCTGTTCGGCAAGAAGGAGCAGACTCCGGTCAAGGGC > AE015451/1499197‑1499769 || gcggggaaCTGCGGTTGTCGCGGAGCTTGGCCGACGGCAAGAGTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGtt < 2:455222/295‑1 (MQ=255) ggAGCAACGGCTGTGGGCGCGCAGCTTCGCCGACGGCAAGGGTGCTGCGGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttc < 2:6955/300‑2 (MQ=255) aGCAACTGCTGTTGTCGCGCAGCTTCGCCGACGGCAAGAGTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGGCGGGGttagg > 1:573891/1‑298 (MQ=255) cAACTGCTGTTGTCGCGCAGCTTCGCCGACGGCAAGAGTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgg > 4:756138/1‑296 (MQ=255) aaCTGCTGTTGTCGCGCAGCTTCGCCGACGGCAAGAGTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcggg < 4:84852/301‑7 (MQ=255) gttgtCGCGCAGCTTCGCCGACGGCAAGAGTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGTCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAcg‑c‑cc < 3:532677/301‑2 (MQ=255) ttgtCGCGCAGCTTCGCCGACGGCAAGAGTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAcg‑c‑ccg < 1:214217/301‑3 (MQ=255) ttgtCGCGCAGCTTCGCCGACGGCAAGAGTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAcg‑c‑ccg < 4:753152/301‑3 (MQ=255) cgcAGCTTGGCGGACGGCAAGAGTGCTGGGCCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCca < 1:386824/301‑1 (MQ=255) cgcAGCTTCGCCGACGGCAAGAGTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTcccc > 4:355088/1‑300 (MQ=255) gcaggtggcgcggtgtcAAGAGTGCTGGCGCCGTGAGCAAGCCGCGGCAGCAAGGGTGCGAGGCCGGTGTCCGCATCGAAGGCCTGAGCTTCGGCATGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCcac < 2:615685/285‑1 (MQ=255) gttcgcagacgggacgaggggtggggcCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGAc < 2:246646/276‑1 (MQ=255) gCCGACGGCAAGAGTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCGGTGAAACAGCAGCCGCGGTGCATGGCCCCGGCTTTGCCGGTGTTCGAGGGCACGCC‑CGCTTCCACAGATTGAt > 3:562171/1‑301 (MQ=255) gCAAGAGTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCGGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACG‑C‑CGCccccccacaccttactccgctt > 2:374630/1‑283 (MQ=255) gcCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCg < 1:46408/301‑1 (MQ=255) agcaagcaGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAAG‑CTCGCTCCCACATACTGATTGCCATTAAGCGGGCCTATCGCTACAg > 1:294692/1‑301 (MQ=255) agcagcGGCAGCAAGGTGGCGAGGCCGATGTCCGAATCGAAGGCATGAGCTTCGGCCGGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑Ac < 1:562156/301‑1 (MQ=255) agcTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAAGAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCCCTACAGTG‑AC‑gcc > 1:615777/1‑299 (MQ=255) caAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACA‑TGCAC‑GGCCCG‑ttttt > 4:64793/1‑301 (MQ=255) aaGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCGCCCAGCGACTGATTGCCATTAAGCGGGCCTATCGGCACAGTC‑ACTGGCCGG‑ttttt > 2:15955/1‑301 (MQ=255) cGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCt < 3:141582/301‑1 (MQ=255) ccGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTa < 4:15955/301‑1 (MQ=255) cccgatctgAAGGCCTGGGCTTCGGCCTGTGGCTCAAGGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAAt < 2:489397/293‑1 (MQ=255) gagcgagggCCTGAGCTTTGGCGTGTGGGTCAATGGCGGGCAGGTTGCCAACGGGCCGCAGTTCGACAACGCTGAGGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATg < 2:228756/294‑1 (MQ=255) cATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATg > 4:427326/1‑301 (MQ=255) aTCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTTCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑GTTTTTGTTTCCTCGCTAACGCATCCTGa > 2:52068/1‑301 (MQ=255) cGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGTACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACGGACTGCCATTAAGCGGGCCTATGGCCAAAGTG‑AC‑GGCCCG‑CTCTTTGTTTCCTCGcccagcaatcattgac > 2:98380/1‑286 (MQ=255) cGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑GTTTTTGTTGCCTCGCTAACGAATCATGAcc > 4:171062/1‑300 (MQ=255) gAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACCGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAAtagatcggg > 4:489514/1‑293 (MQ=255) gggCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTccc < 3:206113/300‑1 (MQ=255) gggCCTGAGCTTCGGCCTGTGGCTAAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTAGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTccc < 3:573906/300‑1 (MQ=255) aGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCCCCCACAGACTGATTGCCATTAAGCGGGCCTTCCGCTACAGTT‑AC‑GTCCCGTTTTTTTGTT‑CCTCGCTaaacaaacattgctccc > 2:48216/1‑286 (MQ=255) ggCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCTGGCCTATCGCTACAGTG‑AC‑GGCCCG‑CTTTTTGTTGCCTCGCTAACGAATCATGACtaccc > 1:424458/1‑297 (MQ=255) ttCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCt < 4:52080/301‑1 (MQ=255) tCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTTGCTCGCTAACGAATCATTACTCCCTTAGAACGCTATCaaacatatat > 1:152969/1‑293 (MQ=255) tCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCt > 1:194063/1‑301 (MQ=255) ggCGAGCGGGTTGGCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAAc < 2:171031/301‑1 (MQ=255) gCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTAGCACCACAGCAGGACTGAAGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTGGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTccc < 4:98400/301‑1 (MQ=255) gaGGTTGCAGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCg < 4:374703/300‑1 (MQ=255) ggTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGAc > 3:369784/1‑301 (MQ=255) ggCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGGGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAAGAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGa < 3:615792/301‑1 (MQ=255) caattCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTCGTTGCCTCGCTAACGCATCATGACTCCCTTAGAACGCTATCAAGCAACTCTGAAACGTCCCGACTTCTTCCATGACgcgg > 3:144267/4‑301 (MQ=255) cAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTCGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACgcgg > 3:144259/1‑301 (MQ=255) cGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAg < 3:424469/301‑1 (MQ=255) aaGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTTCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAACAAGCAGATCTGAAACGTCCCGACTTCTTCCCTGACGCGGCGCAGGacacctcggg > 2:483664/1‑292 (MQ=255) aGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTg < 3:294698/301‑1 (MQ=255) ggCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCCCCTCCac > 1:119814/1‑300 (MQ=255) ttCAGCGCCTGAAAGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCt < 2:755922/301‑1 (MQ=255) gagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTac > 4:494282/1‑301 (MQ=255) ggcctgcaagaggCCTTGGCACAAACGCAGGACTGGGGCTGCCAAACGGGGGGGGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTacg < 2:98309/289‑1 (MQ=255) cTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGCCTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTacgacg > 4:86348/1‑301 (MQ=255) ggCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTg < 2:86329/300‑1 (MQ=255) ccTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCTGCCCAGGAAACTGCGGTCCGTCCCCTCCCGCGCCCTTACGACGACCTGGTGcc > 2:215914/1‑300 (MQ=255) ccTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCCGCGCAGGAAACTGCGGTTAGTCACCTCCAGCCCCTGTaccacccactggcgcc > 2:215911/1‑287 (MQ=255) ggACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAACAAg < 4:483781/301‑1 (MQ=255) cTGAGGCTGCCAAACGGTGGGGGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAACAAGCCg < 4:48228/301‑1 (MQ=255) cTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAACAAGCCg < 4:371580/301‑1 (MQ=255) gCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAACAAGCCGGGCAt < 3:119818/301‑1 (MQ=255) ccAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGCCCGGGTGACCGCGCAGAACAAAAAGCCGGGCCTGttt > 1:417781/1‑300 (MQ=255) ccAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAACAAGCCGGGCATGTTc < 3:194065/301‑1 (MQ=255) aaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTCCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCCCGCGCAGAACAACAAGCCGGGCATGTTCgcc > 3:468770/1‑299 (MQ=255) aaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑CTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATCACGCGTCGCAGGAAACTTCGGTGCGTCAACTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAACACGCCGTGAATGtttggg > 4:483109/1‑297 (MQ=255) cGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAACAAGCCGGGCATGTTCGGCaa < 3:417791/301‑1 (MQ=255) gCGGGCGTGCCAGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAACAAGCCGGGCATGTTCGGCAAGCTGTTCg < 2:427224/301‑1 (MQ=255) cGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGACACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAACAAGCCGGGCATGTTCGGCAAGCTGTTCgg > 3:404774/1‑301 (MQ=255) ctggctcgcgAAACAGGCGCCGCCGTGCATGGCACCGGCGTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAACAAGCCGGGCATGTTCGGCAAGCTGTTCGGCaag < 2:494165/295‑1 (MQ=255) gTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCACGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAACAAGCCGGGCATGTTCGGCAAGCTGTTCGGCaaga > 4:319934/1‑301 (MQ=255) gCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAGCAAGCCGGGCATGTTCGGCAAGCTGTTCGGCaagaag < 4:215949/301‑1 (MQ=255) gCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAACAAGCCGGGCATGTTCGGCAAGCTGTTCGGCaagagg < 4:215952/301‑3 (MQ=255) cgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAACAAGCCGGGCATGTTCGGCAAGCTGTTCGGCAAGAAGGAGCa > 4:587865/1‑301 (MQ=255) cAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTAGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACTTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCCTCCCCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAACAAGCCGGGCATTTTCGGACAGCTGTTCGGCAAGAAGGAGCAGACTc > 1:436949/1‑301 (MQ=255) aGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAACAAGCCGGGCATGTTCGGCAAGCTGTTCGGCAAGAAGGAGCAGACTcc > 1:640106/1‑301 (MQ=255) gTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAACAAGCCGGGCATGTTCGGCAAGCTGTTCGGCAAGAAGGAGCAGACTCCGGTCAAGGGc > 4:83149/1‑301 (MQ=255) || GCGAGCAACTGCTGTTGTCGCGCAGCTTCGCCGACGGCAAGAGTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCACG‑C‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTG‑AC‑GGCCCG‑TTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAACAAGCCGGGCATGTTCGGCAAGCTGTTCGGCAAGAAGGAGCAGACTCCGGTCAAGGGC > AE015451/1499197‑1499769 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |