| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AE015451 | 3,137,784 | (T)9→8 | intergenic (+212/‑78) | PP_2753 → / → PP_2754 | putative Branched‑chain amino acid ABC transporter, ATP‑binding protein/putative OprD family outer membrane porin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 3,137,776 | 0 | T | . | 98.0% | 224.7 / ‑1.5 | 50 | intergenic (+204/‑86) | PP_2753/PP_2754 | putative Branched‑chain amino acid ABC transporter, ATP‑binding protein/putative OprD family outer membrane porin |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base . (24/25); total (24/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01 | |||||||||||
CATCCTGGAAAACGGCCGGGTGGTAGGTGAGGGCAGTGCTGCCGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGTTTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAATTTCTTCTTCAGCCAGGACTATCGCAACGGCGACTTTACACTCAATCCCAATAATGGCGAGCGGCAGAGCCTGCATCGTGAATGGG > AE015451/3137476‑3138072 | cATCCTGGAAAACGGCCGGGTGGTAGGTGAGGGCAGTGCTGCCGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGt > 4:650651/1‑301 (MQ=255) aaaCGGCCGGGTGGTAGGTGAGGGCAGTGCTGCCGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGttttttttgt > 3:221957/1‑299 (MQ=255) aaCGGCGGGGTGGGAGGGGAGGGCGGTGCTGCGGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGttttttttgtt < 2:183086/301‑4 (MQ=255) aCGGCCGGGTGGTAGGTGAGGGAAGTGCTGCCGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGttttttttgttg < 4:303943/301‑5 (MQ=255) gcGGGGGGTAGGGGAGGGCAGGGCTGCCGAGCTGGCCGGGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCAt < 2:650440/300‑1 (MQ=255) gtggtAGGTGAGGGCAGTGCTGCCGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGgtt < 1:188689/301‑1 (MQ=255) gtgtgctcttccgatctccGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACggg < 3:56140/284‑1 (MQ=255) ggAGCTGGCCGGGCGGGAAGATATTCAGCATGTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAgc < 2:726649/300‑1 (MQ=255) tGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTg < 4:618029/301‑1 (MQ=255) ccGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGagatcggaagagcgtcg > 1:56138/1‑285 (MQ=255) cgGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGgcg > 2:783830/1‑301 (MQ=255) atatTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCt < 3:543587/301‑1 (MQ=255) atTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCg < 4:784110/301‑1 (MQ=255) atcttACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCa < 4:42199/297‑1 (MQ=255) tACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCTTGCAACGGCGCCTAGCTCGTTATTCCAAgag > 2:42188/1‑299 (MQ=255) gggCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACt < 4:344283/301‑1 (MQ=255) cAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGTTTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCAt < 4:489366/301‑1 (MQ=255) aaGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATgc > 3:353548/1‑301 (MQ=255) gCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTg < 1:221959/301‑1 (MQ=255) cGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGcaca > 2:318588/1‑301 (MQ=255) cTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGcc > 1:375323/1‑300 (MQ=255) cagacgtgtgctcttccgatcttAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCAt < 4:259862/279‑1 (MQ=255) aGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCAt > 3:156243/1‑301 (MQ=255) gTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATc < 1:353547/301‑1 (MQ=255) cATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATTCGCTCTACCGTTTGCTACAGCACCGCTCTTTCTCTGGCCATCct > 2:149399/1‑301 (MQ=255) tgTAGGTGATGTTCCATTCAACGCGGCCCAGGCTGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCtt < 1:609480/300‑1 (MQ=255) gTAGGTGATGTTCCATTCAACGCGGCCCAGGCTGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCtta > 3:609482/1‑300 (MQ=255) tAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCAtagatcggcagagcgtcgtgtag > 2:259804/1‑279 (MQ=255) gggTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTg < 1:156241/300‑1 (MQ=255) tGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACa < 4:318661/301‑1 (MQ=255) aCGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGaa < 3:375323/301‑1 (MQ=255) gCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAATGCtt > 2:685930/1‑301 (MQ=255) ccGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGAc < 4:686160/301‑1 (MQ=255) ccacggcgctcttccgatcggAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTGGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACg < 2:141649/281‑1 (MQ=255) gTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGcc > 3:114082/1‑300 (MQ=255) gTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGcc > 2:715078/1‑300 (MQ=255) tgagacgtgtgcacttccgatctcggtgGCGCAGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGAGCTGGAGCGCCTGGAAAGGCGCCTACCTCGTCATGCCAAGGATCACTGCCAGGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCAc < 4:62447/276‑1 (MQ=255) gAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACgagatcggaagagcacacgtc > 4:141678/1‑281 (MQ=255) tgcTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCg < 4:360242/300‑1 (MQ=255) gcTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCACCCgg > 2:360158/1‑300 (MQ=255) cTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTTCTGGCCAGCACCGCTTTTGCCGCCAGCGATGATGAAGGCTTCATCGACGGCAcagatcggaatagcgtcgggtagg > 2:62430/1‑278 (MQ=255) cctttgccctacacgacgctcttccgatctGCTTCGCAGACGTAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAAt < 2:718647/272‑1 (MQ=255) ggCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAAttt < 1:114079/301‑1 (MQ=255) ttGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAATTTCTTCTTCAGCCAGGACt > 3:39514/1‑301 (MQ=255) gCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAAtagatcggaagagcacacgtctgaactccag > 4:718892/1‑271 (MQ=255) gCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAATTTCTTCTTCAGCCAGGACTATCGCAACGGc > 4:225460/1‑301 (MQ=255) catAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAATTTCTTCTTCAGCCAGGACTATCGCAACGGCGACTTTACCATCAATccc > 2:671504/1‑301 (MQ=255) tgtTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCTCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAATGCTTCATCGACGGCACGCGGGTCCCTGTTCTCAACCGCAATTTCTTCTTCAGCCAGGACTATCTCAACGTCTACTTTACCCTCAATCCCaatac > 2:267464/1‑300 (MQ=255) ttGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAATTTCTTCTTCAGCCAGGACTATCGCAACGGCGACTTTACACTCAATCCCAATAATg < 4:715323/301‑1 (MQ=255) gttgttCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAATTTCTTCTTCAGCCAGGACTATCGCAACGGCGACTTTACACTCAATCCCAATAATGGCGAGCgg > 1:351536/1‑301 (MQ=255) tGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAATTTCTTCTTCAGCCAGGACTATCGCAACGGCGACTTTACACTCAATCCCAATAATGGCGAGCGGCAGAGCCTGCATCg > 1:250058/1‑301 (MQ=255) aaTGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAATTTCTTCTTCAGCCAGGACTATCGCAACGGCGACTTTACACTCAATCCCAATAATGGCGAGCGGCAGAGCCTGCATCGTGAATggg > 2:412073/1‑301 (MQ=255) aaTGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAATTTCTTCTTCAGCCAGGACTATCGCAACGGCGACTTTACACTCAATCCCAATAATGGCGAGCGGCAGAGCCTGCATCGTGAATggg < 4:149437/301‑1 (MQ=255) aaTGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAATTTCTTCTTCAGCCAGGACTATCGCAACGGCGACTTTACACTCAATCCCAATAATGGCGAGCGGCAGAGCCTGCATCGTGAATggg < 4:671726/301‑1 (MQ=255) | CATCCTGGAAAACGGCCGGGTGGTAGGTGAGGGCAGTGCTGCCGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGTTTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAATTTCTTCTTCAGCCAGGACTATCGCAACGGCGACTTTACACTCAATCCCAATAATGGCGAGCGGCAGAGCCTGCATCGTGAATGGG > AE015451/3137476‑3138072 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |