Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451	1,419,549	+C	intergenic (‑38/+98)	PP_1242 ← / ← PP_1244	conserved protein of unknown function/conserved protein of unknown function

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	1,419,548	1	.	C	100.0%	46.8 / NA	14	intergenic (‑37/+99)	PP_1242/PP_1244	conserved protein of unknown function/conserved protein of unknown function
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (6/8);  total (6/8)

CATCCCCAACTCGGCATCATCCAGCAGCGCCTTGGCCATCGCGCTCAGGTAATGCGAGGCCCAGATCAGTTTCTGGTCGCCGTCCATCAGGCCGGTGATGATCAGGTCGCGCACGTAACCCATCAGTTCGGAAGCCTGTTCGCGGGCGTCCTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGG‑CTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTAAATGCGACCGCATCTGCAACTGCATCAACGCCGACTCGACCAGCCCCTGTACCGCTTCCATTTCATGGATCACGGCCTGCATCACGATCGAGCTGTGGGCGGTGGGTTTGAGTTGCACGGATTGGCGGGCCACGGACAGGGCGCAGAGTACGTACTCTGAGG  >  AE015451/1419251‑1419837                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                  catcCCCAACTCGGCATCATCCAGCAGCGCCTTGGCCATCGCGCTCAGGTAATGCGAGGCCCAGATCAGTTTCTGGTCGCCGTCCATCAGGCCGGTGATGATCAGGTCGCGCACGTAACCCATCAGTTCGGAAGCCTGTTCGCGGGCGTCCTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGTTTGCCTGggctgg‑ccc                                                                                                                                                                                                                                                                                                >  1:81103/1‑299 (MQ=255)     cccAACTCGGCATCATCCAGCAGCGCCTTGGCCATCGCGCTCAGGTAATGCGAGGCCCAGATCAGTTTCTGGTCGCCGTCCATCAGGCCGGTGATGATCAGGTCGCGCACGTAACCCATCAGTTCGGAAGCCTGTTCGCGGGCGTCCTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTc                                                                                                                                                                                                                                                                                             <  3:266449/301‑1 (MQ=255)           tCGGCATCATCCAGCAGCGCCTTGGCCATCGCGCTCAGGTAATGCGAGGCCCAGATCAGTTTCTGGTCGCCGTCCATCAGGCCGGTGATGATCAGGTCGCGCACGTAACCCATCAGTTCGGAAGCCTGTTCGCGGGCGTCCTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCgggg                                                                                                                                                                                                                                                                                       >  3:511492/1‑300 (MQ=255)                             gcCTTGGCCATCGCGCTCAGGTAATGCGAGGCCCAGATCAGTTTCTGGTCGCCGTCCATCAGGCCGGTGATGATCAGGTCGCGCACGTAACCCATCAGTTCGGAAGCCTGTTCGCGGGCGTCCTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGt                                                                                                                                                                                                                                                                     <  1:112363/301‑1 (MQ=255)                                       tCGCGCTCAGGTAATGCGAGGCCCAGATCAGTTTCTGGTCGCCGTCCATCAGGCCGGTGATGATCAGGTCGCGCACGTAACCCATCAGTTCGGAAGCCTGTTCGCGGGCGTCCTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGt                                                                                                                                                                                                                                                           <  3:81105/301‑1 (MQ=255)                                                    aTGCGAGGCCCAGATCAGTTTCTGGTCGCCGTCCATCAGGCCGGTGATGATCAGGTCGCGCACGTAACCCATCAGTTCGGAAGCCTGTTCGCGGGCGTCCTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCtgt                                                                                                                                                                                                                                              >  3:271711/1‑301 (MQ=255)                                                                               gcggTCCATCAGGCCGGTGATGCTCAGGTCTCGCACGTAACCCGTCCGTTCTGAAGCCTGTTCGCGGGCGGCCTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGccc                                                                                                                                                                                                                   <  2:345193/298‑1 (MQ=255)                                                                               gNNNTCCNTCAGGCCGGTGATGATCAGGTCGCGCACGTAACCCATCAGTTCGGAAGCCTGTTCGCGGGCGTCCTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTATGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCTCGGGCACGCCCGCTCCCACAGGTACCGTGCAGTGCGTGACGGCTGTGTTGAGCCTGTGGGCGCGGGCGTgtgc                                                                                                                                                                                                                   >  1:519668/1‑298 (MQ=255)                                                                                            ccGGTGATGATCAGGTCGCGCACGTAACCCATCAGTTCGGAAGCCTGTTCGCGGGCGTCCTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTg                                                                                                                                                                                                      <  1:511490/301‑1 (MQ=255)                                                                                                                              tttCGGAAGCCTGTTCGCGGGCGTCCCGGCAGGGGATGGCGGGGTCGATTCGGAACAGGGGGTGGGTGTTGTTTTCGCCTTTGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTGCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCCGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTa                                                                                                                                                                    <  4:258804/300‑1 (MQ=255)                                                                                                                                              gcgGGCGTCCTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTAAATGCGACCGCATCTg                                                                                                                                                    <  1:271712/301‑1 (MQ=255)                                                                                                                                                                                cGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTAAATGCGACCGCATCTGCAACTGCATCAACGCCGACTCGACCAGCCCCTGt                                                                                                                  >  3:219108/1‑301 (MQ=255)                                                                                                                                                                                                                          gTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTAAATGCGACCGCATCTGCAACTGCATCAACGCCGACTCGACCAGCCCCTGTACCGCTTCCATTTCATGGATCAAGGCCTGCATCCCGATCGAg                                                                        >  3:469447/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                   tGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTAAATGCGACCGCATCTGCAACTGCATCAACGCCGACTCGACCAGCCCCTGTACCGCTTCCATTTCATGGATCACGGCCTGCATCACGATCGAGCTGTGGGCGGTGGGTTTGAGTTGCACGGATTGGCGGGCCACGGACAGGGCGCagagg               >  3:497790/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                gCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCAACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTAAATGCGACCGCATCTGCAACTGCATCAACGCCGACTCGACCAGCCCCTGTACCGCTTCCATTTCATGGATCACGGCCTGCATCACGATCGAGCTGTGGGCGGTGGGTTTGAGTTGCACGGATTGGCGGGCCACGGACAGGGCGCAGAGTACGTACTCTGAgg  <  1:219110/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                  CATCCCCAACTCGGCATCATCCAGCAGCGCCTTGGCCATCGCGCTCAGGTAATGCGAGGCCCAGATCAGTTTCTGGTCGCCGTCCATCAGGCCGGTGATGATCAGGTCGCGCACGTAACCCATCAGTTCGGAAGCCTGTTCGCGGGCGTCCTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGG‑CTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTAAATGCGACCGCATCTGCAACTGCATCAACGCCGACTCGACCAGCCCCTGTACCGCTTCCATTTCATGGATCACGGCCTGCATCACGATCGAGCTGTGGGCGGTGGGTTTGAGTTGCACGGATTGGCGGGCCACGGACAGGGCGCAGAGTACGTACTCTGAGG  >  AE015451/1419251‑1419837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑