Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451 5,674,751 +G intergenic (+113/+27) rhlE‑II → / ← yceI ATP‑dependent RNA helicase/polyisoprene‑binding protein, acidic stress response factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154515,674,7501.G98.0% 184.6 / ‑3.4 51intergenic (+112/+28)rhlE‑II/yceIATP‑dependent RNA helicase/polyisoprene‑binding protein, acidic stress response factor
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (22/28);  total (22/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

CGCCCTGCGCGTGACGATGCTGCCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑GCGCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCATGGTCAGGTCGCCAGTCACGTCAGCCGTCTTGTCGCCAGTAGACTTGACGCTGGTGGAGACGAATTTGGCGT  >  AE015451/5674453‑5675043
                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                       
cGCCCTGCGCGTGACGATGCTGCCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAgc‑ggg                                                                                                                                                                                                                                                                                                      >  1:26077/1‑299 (MQ=255)
  cccTGCGCGTGACGATGCTGCCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACTTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAgc‑ggccc                                                                                                                                                                                                                                                                                                    >  3:238028/1‑297 (MQ=255)
             ccgaggctgcCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTc                                                                                                                                                                                                                                                                                        <  1:43749/296‑1 (MQ=255)
                           aaaGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAAtt                                                                                                                                                                                                                                                                          <  3:54828/300‑1 (MQ=255)
                            aaGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAAttt                                                                                                                                                                                                                                                                         >  1:54826/1‑300 (MQ=255)
                             agagCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAACGCGGCCCC‑‑CTCGGTTTTCAGATGGCAGAAATTAc                                                                                                                                                                                                                                                                        >  2:100532/1‑301 (MQ=255)
                             agagCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTAc                                                                                                                                                                                                                                                                        <  3:608644/301‑1 (MQ=255)
                                      ccgatctaaCCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTc                                                                                                                                                                                                                                                               <  3:85059/294‑1 (MQ=255)
                                           gcAACCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACAcc                                                                                                                                                                                                                                                          <  1:204329/301‑1 (MQ=255)
                                             aaCCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTcagatcgg                                                                                                                                                                                                                                                        >  1:85056/1‑295 (MQ=255)
                                             aaCCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCtt                                                                                                                                                                                                                                                        <  4:100553/301‑1 (MQ=255)
                                                   cgTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGacac                                                                                                                                                                                                                                                  <  1:1143/301‑1 (MQ=255)
                                                   cgTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGacac                                                                                                                                                                                                                                                  <  4:589288/301‑1 (MQ=255)
                                                   cgTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGacac                                                                                                                                                                                                                                                  >  3:1143/1‑301 (MQ=255)
                                                        gCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGata                                                                                                                                                                                                                                             >  4:701196/1‑301 (MQ=255)
                                                                      cagggagcATTCGCAAGGGGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGt                                                                                                                                                                                                                               <  2:644173/294‑1 (MQ=255)
                                                                       aGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTc                                                                                                                                                                                                                              >  1:632016/1‑301 (MQ=255)
                                                                            tCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGa                                                                                                                                                                                                                         <  3:632018/301‑1 (MQ=255)
                                                                                        ggTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACTTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCgg                                                                                                                                                                                                             <  1:238030/300‑1 (MQ=255)
                                                                                         gTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATATGGCCGCAACGCGGCCCC‑‑CTCGGCTTTCCGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGCAGTAGCACCCgtg                                                                                                                                                                                                            >  4:722587/1‑299 (MQ=255)
                                                                                           ccAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGcc                                                                                                                                                                                                          >  3:110208/1‑301 (MQ=255)
                                                                                             aagcacgACCGTTTCCCGACGCCGGAGCAACACGATGACCTGCCAAGCCGCCCACGCGGTGGGCGACCAACGCAGCTGACCCGCAATTGCTCATAGAAGCACCAAGAG‑GGCTGCTTCGCATCCCAATCACGAAACAAGGCCGCTCCTGCAGTGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTAGTGATAGGGCCGCAAAGC‑G‑GCCGACTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCtt                                                                                                                                                                                                        <  2:722342/295‑1 (MQ=255)
                                                                                              gacgtgtgctcttccgatctccTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCttt                                                                                                                                                                                                       <  4:153719/281‑1 (MQ=255)
                                                                                               ggtGGATCGTTTCCCGCGGCCGTAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTg                                                                                                                                                                                                      <  2:717515/299‑1 (MQ=255)
                                                                                                cTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGa                                                                                                                                                                                                     >  4:566343/1‑301 (MQ=255)
                                                                                                                  ccTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCCCCCGGGCCtttagagcggaagagcgtcgtgt                                                                                                                                                                                   >  2:153681/1‑281 (MQ=255)
                                                                                                                     gAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggttca                                                                                                                                                                                <  1:110205/301‑1 (MQ=255)
                                                                                                                         cacggaacgcccggcgaggctgccCACGCAGTGCGCGCCCGGCGCTGCTGACCCGCAATGGCTGTAAGAATTACC‑AGGGTGGCTCCTTCGCAGCCCTATCACGACGCAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgt                                                                                                                                                                            <  2:700951/280‑1 (MQ=255)
                                                                                                                            tGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggg                                                                                                                                                                         >  1:18137/1‑300 (MQ=255)
                                                                                                                                                      ggTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGc                                                                                                                                               >  4:293998/1‑301 (MQ=255)
                                                                                                                                                            ggcCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTCCCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCAcccc                                                                                                                                         <  4:623111/300‑1 (MQ=255)
                                                                                                                                                                   ggctgctGACCCGCAAGGGCTGGTAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTc                                                                                                                                  <  2:566173/300‑1 (MQ=255)
                                                                                                                                                                      cgctgACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCCCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAAATACTTCTGCTTCACCCCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCtt                                                                                                                               <  4:204643/300‑1 (MQ=255)
                                                                                                                                                                                       tGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAgg                                                                                                              <  3:26077/301‑1 (MQ=255)
                                                                                                                                                                                          aaGAAGTACCGAGGC‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAg                                                                                                           <  1:564469/300‑1 (MQ=255)
                                                                                                                                                                                                         tcagacgtgtgctcttccgatctgACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCAcc                                                                                             <  4:728601/278‑1 (MQ=255)
                                                                                                                                                                                                               ctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTg                                                                                       <  3:18137/301‑1 (MQ=255)
                                                                                                                                                                                                                ttCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTgg                                                                                      >  1:74732/1‑301 (MQ=255)
                                                                                                                                                                                                                  tccccgccccccttccgatcttgcctcTCCTGAAGAGAATTTCTTGATCTCTACAGGGGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTg                                                                                    <  2:370893/275‑1 (MQ=255)
                                                                                                                                                                                                                                gACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCAccagatcggaagagcgtcgggtagg                                                                      >  2:728341/1‑278 (MQ=255)
                                                                                                                                                                                                                                gACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCATGGt                                                                      >  3:597603/1‑301 (MQ=255)
                                                                                                                                                                                                                                       ggCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGagatcggaagaggacacgtcc                                                               >  4:370983/1‑281 (MQ=255)
                                                                                                                                                                                                                                              ccTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCATGGTCAGGTCGCCAGTcc                                                        >  4:528657/1‑300 (MQ=255)
                                                                                                                                                                                                                                               agttcagacgtgtgctcttccgatctggAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCATGGTCAGGTCGCCAGTCAc                                                       <  3:29868/275‑1 (MQ=255)
                                                                                                                                                                                                                                                   cttgaATTTCTTGATCTCTACAGGAGCGGCCTTGGGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAGCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCATGGTCAGGTCGCCAGTCACGTCa                                                   <  2:126478/298‑1 (MQ=255)
                                                                                                                                                                                                                                                     agaATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCATGGTCAGGTCGCCAGTCACGTCaag                                                 >  4:126507/1‑299 (MQ=255)
                                                                                                                                                                                                                                                       aaTTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCATGGTCAGGTCGCCAGTCACGTCAGCCg                                               <  3:74734/301‑1 (MQ=255)
                                                                                                                                                                                                                                                              gacgctcttccgatctggCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCATGGTCAGGTCGCCAGTCACGTCAGCCGTCTTGTc                                        <  2:179786/285‑1 (MQ=255)
                                                                                                                                                                                                                                                                       cAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCATGGTCAGGTCGCCAGTCACGTCAGCCGTCTTGTCGCCAGTAgg                               >  4:550916/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                         ggAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCATGGTCAGGTCGCCAGTCAcagatcggaagagcgtcgtgtagggaa                             >  1:29868/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                              ggCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCATGGTCAGGTCGCCAGTCACGTCAGCCGTCTTGTcagatcggaagagcaca                        >  4:179828/1‑285 (MQ=255)
                                                                                                                                                                                                                                                                                                  ccGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCATGGTCAGGTCGCCAGTCACGTCAGCCGTCTTGTCGCCAGTAGACTTGACGCTGGTGGAGACGAATTTGGc    <  1:597601/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                    gCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAGGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCATGGTCAGGTCGCCAGTCACGTCAGCCGTCTTGTCGCCAGTAGACTTGACGCTGGTGGAGACGAATTTGGCGt  >  4:620234/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                       
CGCCCTGCGCGTGACGATGCTGCCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑GCGCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCATGGTCAGGTCGCCAGTCACGTCAGCCGTCTTGTCGCCAGTAGACTTGACGCTGGTGGAGACGAATTTGGCGT  >  AE015451/5674453‑5675043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑