Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	AE015451	2,032,505	1680 bp→GCGGCCGC		[pgi‑I]	[pgi‑I]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	AE015451	2032505	2034184	1680	63 [1]	[0] 57	[pgi‑I]	[pgi‑I]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 2032504	1 (0.010)	57 (0.830) +GCGGCCGC	48/584	0.2	99.2%	intergenic (‑260/‑16)	kdsA‑II/pgi‑I	2‑dehydro‑3‑deoxyphosphooctonate aldolase/glucose‑6‑phosphate isomerase
	?	AE015451	2034185 =	0 (0.000)					intergenic (+1/+1440)	pgi‑I/PP_5477	glucose‑6‑phosphate isomerase/conserved protein of unknown function

GCCGAGTTGGAGCATTCGATTGTGGAGTTGATCTTGATCATTTTTTACTCGATAGGTCCTTAGGGGGCAACCCGCAGGACATGGCAAAGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCAAGGATCTTTTTGCAATGGCGTTCTACCGTACACCTCACGATGTCACTGCTCTGCCAGCCTGGAAGGCGCTTCAGCAACACCGCGAAGCCATGCGAGGCTTCAGCATGTCCGAAGCCTTTGCCGCCGATGCCAAGCGCTTCGACCAGTTTTCCCTTAGCGCTTGCGGCCTGTTCCTCGACTACTCAAAAAACCTGATCACCGAGCAAAGCCGCGATCTGCTGGTCAACCTGGCCAATGAAGTGGGCCTGCAGGACGCCATCAAGTCGA  >  AE015451/2032204‑2032772                                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                             tccgatctGGAGCATTCGATTGTGGAGTTGATCTTGATCATTTTTTACTCGATAGGTCCTTAGGGGGCAACCCGCAGGACATGGCAAAGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATc                                                                                                                                                                                                                                                                              <  3:476081/294‑1 (MQ=255) gCCGAGTTGGGGCATTCGATTGTGGAGTTGATCTTGATCATTTTTTACTCGATAGGTCCTTAGGGGGCAACCCGCAGGACATGGCAAAGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATc                                                                                                                                                                                                                                                                              <  2:204434/301‑1 (MQ=255)   cGAGTTGGAGCATTCGATTGTGGAGTTGATCTTGATCATTTTTTACTCGATAGGTCCTTAGGGGGCAACCCGCAGGACATGGCAAAGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgc                                                                                                                                                                                                                                                                            <  4:11343‑M1/301‑3 (MQ=255)       ttGGAGCATTCGATTGTGGAGTTGATCTTGATCATTTTTTACTCGATAGGTCCTTAGGGGGCAACCCGCAGGACATGGCAAAGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggcc                                                                                                                                                                                                                                                                        >  1:337434‑M1/1‑295 (MQ=255)        tGGAGCATTCGATTGTGGAGTTGATCTTGATCATTTTTTACTCGATAGGTCCTTAGGGGGCAACCCGCAGGACATGGCAAAGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccg                                                                                                                                                                                                                                                                       >  4:55431‑M1/1‑294 (MQ=255)         ggAGCATTCGATTGTGGAGTTGATCTTGATCATTTTTTACTCGATAGGTCCTTAGGGGGCAACCCGCAGGACATGGCAAAGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCagatcggg                                                                                                                                                                                                                                                                      >  1:476079/1‑294 (MQ=255)              aTTCGATTGTGGAGTTGATCTTGATCATTTTTTACTCGATAGGTCCTTAGGGGGCAACCCGCAGGACATGGCAAAGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctg                                                                                                                                                                                                                                                                 >  1:116610‑M1/1‑288 (MQ=255)                 cGATTGTGGAGTTGATCTTGATCATTTTTTACTCGATAGGTCCTTAGGGGGCAACCCGCAGGACATGGCAAAGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgctt                                                                                                                                                                                                                                                              >  3:405451‑M1/1‑285 (MQ=255)                  gATTGTGGAGTTGATCTTGATCATTTTTTACTCGATAGGTCCTTAGGGGGCAACCCGCAGGACATGGCAAAGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttg                                                                                                                                                                                                                                                             >  4:564281‑M1/1‑284 (MQ=255)                                  ttgatcATTTTTTACTCGATAGGTCCTTAGGGGGCAACCCGCAGGACATGGCAAAGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcggg                                                                                                                                                                                                                                             >  3:204409‑M1/1‑268 (MQ=255)                                   tgatcATTTTTTACTCGATAGGTCCTTAGGGGGCAACCCGCAGGACATGGCAAAGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTAACgcggccgcccctgcttgatactggcccggcgggg                                                                                                                                                                                                                                            >  2:585483‑M1/1‑267 (MQ=255)                                            tttACTCGATAGGTCCTTAGGGGGCAACCCGCAGGACATGGCAAAGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtcc                                                                                                                                                                                                                                   <  1:405450‑M1/301‑44 (MQ=255)                                            tttACTCGATAGGTCCGTAGGGGGCAACCCGCAGGACATGGCAAAGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtcc                                                                                                                                                                                                                                   <  3:116612‑M1/301‑44 (MQ=255)                                                  cGATAGGTCCTTAGGGGGCAACCCGCAGGACATGGCAAAGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggtccggcgggaggagcgtccgagcgg                                                                                                                                                                                                                             >  4:547575‑M1/1‑252 (MQ=255)                                                         tCCTTAGGGGGCAACCCGCAGGACATGGCAAAGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggt                                                                                                                                                                                                                      <  1:204411‑M1/301‑57 (MQ=255)                                                             tAGGGGGCAACCCGCAGGACATGGCAAAGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtg                                                                                                                                                                                                                  >  3:158899‑M1/1‑241 (MQ=255)                                                                       cccGCAGGACATGGCAAAGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaaca                                                                                                                                                                                                        >  1:618572‑M1/1‑231 (MQ=255)                                                                        ccGCAGGACATGGCAAAGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacac                                                                                                                                                                                                       <  3:618574‑M1/301‑72 (MQ=255)                                                                                    gCAAAGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatg                                                                                                                                                                                           >  2:569111‑M1/1‑218 (MQ=255)                                                                                       ggtgctcttccgatctcgcgcccttgctaaccgtggtTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcg                                                                                                                                                                                        <  4:567585‑M1/285‑87 (MQ=255)                                                                                       cttccgatctagtcagcgcgCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcg                                                                                                                                                                                        <  1:449644‑M1/292‑87 (MQ=255)                                                                                        aGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCGTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcggggggagtgtccgggcggagctggtggtcgccggcagcactagcgccgcatggctg                                                                                                                                                                                       >  2:347953‑M1/1‑214 (MQ=255)                                                                                                 aGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcgagatcggaag                                                                                                                                                                              >  3:449646‑M1/1‑205 (MQ=255)                                                                                                   tCAGCGCGCCCTTGCTAACCGGGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcagg                                                                                                                                                                            <  2:564111‑M1/301‑99 (MQ=255)                                                                                                       cgcgcCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcagggcgaggtcggaaagggcgc                                                                                                                                                                        >  2:567415‑M1/1‑199 (MQ=255)                                                                                                         cgcCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcat                                                                                                                                                                      <  4:585668‑M1/301‑105 (MQ=255)                                                                                                           cccTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcattt                                                                                                                                                                    <  4:569281‑M1/301‑107 (MQ=255)                                                                                                           cccTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcattt                                                                                                                                                                    <  3:111324‑M1/301‑107 (MQ=255)                                                                                                            gtgtgctcttccgatctgtgccgattctacaattttTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttc                                                                                                                                                                   <  4:711200‑M1/285‑108 (MQ=255)                                                                                                               tGCTAACCGGGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgac                                                                                                                                                                <  2:55414‑M1/301‑111 (MQ=255)                                                                                                                   aaCCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgaagcag                                                                                                                                                            >  4:258945‑M1/1‑187 (MQ=255)                                                                                                                       gTGGTTGTGCCGATTCTACAATTTGTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggt                                                                                                                                                        <  4:650059‑M1/301‑119 (MQ=255)                                                                                                                            tgtgCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtac                                                                                                                                                   <  1:158897‑M1/301‑124 (MQ=255)                                                                                                                             gtgCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcggcggaaacactagcgcatcatggcggggaatagcaggccgcatttcagatcggaagagcgtcg                                                                                                                                                  >  2:710955‑M1/1‑177 (MQ=255)                                                                                                                              gtccttttttacaatttttCTGCTGCCTTTCGAGCATGTCGAGGCTGCCAGGCGTTTGTTAGCTGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACTATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacag                                                                                                                                                 <  2:258887‑M1/292‑126 (MQ=255)                                                                                                                                ccGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtc                                                                                                                                               <  4:284147‑M1/301‑128 (MQ=255)                                                                                                                                          aaTTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtag                                                                                                                                     <  3:337435‑M1/301‑138 (MQ=255)                                                                                                                                              ccctacacgacgctcttccgatctggggctgccaggcgtttgtgagcggtatttTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaa                                                                                                                                 <  2:269969‑M1/275‑142 (MQ=255)                                                                                                                                                ctacacgacgctcttccgatctgaggcttccaggcgtttttgagcGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaatt                                                                                                                               <  1:219168‑M1/279‑144 (MQ=255)                                                                                                                                                       ccTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGGGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggtccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagat                                                                                                                        <  2:547418‑M1/301‑151 (MQ=255)                                                                                                                                                        cTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagatt                                                                                                                       >  4:330233‑M1/1‑150 (MQ=255)                                                                                                                                                             gAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaattgcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtt                                                                                                                  >  3:307735‑M1/1‑145 (MQ=255)                                                                                                                                                                cATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatc                                                                                                               >  3:189527‑M1/1‑142 (MQ=255)                                                                                                                                                                      gAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaaagatcggaagagcacacgtctgaa                                                                                                         >  4:270035‑M1/1‑136 (MQ=255)                                                                                                                                                                      gAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgaagaggtggtacagtcaacagggtaggtaattagatcggaagagcacacgtctg                                                                                                         >  3:219166‑M1/1‑136 (MQ=255)                                                                                                                                                                            gccgggcgttGGTGAGCGGTATTTTGCCCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgct                                                                                                   <  2:330154‑M1/297‑172 (MQ=255)                                                                                                                                                                               aGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaat                                                                                                >  3:629665‑M1/1‑127 (MQ=255)                                                                                                                                                                                ggCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatg                                                                                               >  4:564502‑M1/1‑126 (MQ=255)                                                                                                                                                                                   gTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctc                                                                                            <  4:348037‑M1/301‑179 (MQ=255)                                                                                                                                                                                   gTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCGTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctc                                                                                            <  1:189529‑M1/301‑179 (MQ=255)                                                                                                                                                                                       gtgAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttgg                                                                                        >  3:184342‑M1/1‑119 (MQ=255)                                                                                                                                                                                                                     tttttcCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttggatggggctcagtcccagttgtgactagagg                                                          <  2:564332‑M1/300‑213 (MQ=255)                                                                                                                                                                                                                              ttCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATCGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttggatggggctcagtcccagttgtgactagaggatctgaatt                                                 >  1:24581‑M1/1‑80 (MQ=255)                                                                                                                                                                                                                                       ctctttccctacacgacgctcttccgatctgaacctgacaacgattgataggttctggcttTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttggatggggctcagtcccagttgtgactagaggatctgaatgtacaatgca                                        <  2:120956‑M1/271‑231 (MQ=255)                                                                                                                                                                                                                                                gCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttggatgggtctcagtcccagttgtgactcgaggctctgcatgtccaatgcccttcttctc                               >  2:325508‑M1/1‑62 (MQ=255)                                                                                                                                                                                                                                                gCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttggatggggctcagtcccagttgtgactagaggatctgaatgtacaatgcacttctgcta                               >  2:584767‑M1/1‑62 (MQ=255)                                                                                                                                                                                                                                                    ataATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttggatggggctcagtcccagttgtgactagaggatctgaatgtacaatgcacttctgctaatcg                           <  1:629663‑M1/301‑244 (MQ=255)                                                                                                                                                                                                                                                            gCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttggatggggctcagtcccagttgtgactagaggatctgaatgtacaatgcacttctgctaatcgctgcaatt                   <  4:584952‑M1/301‑252 (MQ=255)                                                                                                                                                                                                                                                                     gAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttggatggggctcagtcccagttgtgactagaggatctgaatgtacaatgcacttctgctaatcgctgcaattgcccttacc          >  3:141241‑M1/1‑41 (MQ=255)                                                                                                                                                                                                                                                                     gAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttggatggggctcagtcccagttgtgactagaggatctgaatgtacaatgcaagatcggaagagcacacgtctgaactccag          >  4:120981‑M1/1‑41 (MQ=255)                                                                                                                                                                                                                                                                     aaacCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttggatggggctcagtcccagttgtgactagaggatctgaatgtacaatgcacttctgctaatcgctgcaattgcccttacc          <  1:184344‑M1/300‑261 (MQ=255)                                                                                                                                                                                                                                                                           gACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttggatggggctcagtcccagttgtgactagaggatctgaatgtacaatgcacttctgctaatcgctgcaattgcccttaccggatgc    <  1:141239‑M1/301‑267 (MQ=255)                                                                                                                                                                                                                                                                             cAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttggatggggctcagtcccagttgtgactagaggatctgaatgtacaatgcacttctgctaatcgctgcaattgcccttaccggctgcgc  >  4:533144‑M1/1‑33 (MQ=255)                                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                             GCCGAGTTGGAGCATTCGATTGTGGAGTTGATCTTGATCATTTTTTACTCGATAGGTCCTTAGGGGGCAACCCGCAGGACATGGCAAAGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCAAGGATCTTTTTGCAATGGCGTTCTACCGTACACCTCACGATGTCACTGCTCTGCCAGCCTGGAAGGCGCTTCAGCAACACCGCGAAGCCATGCGAGGCTTCAGCATGTCCGAAGCCTTTGCCGCCGATGCCAAGCGCTTCGACCAGTTTTCCCTTAGCGCTTGCGGCCTGTTCCTCGACTACTCAAAAAACCTGATCACCGAGCAAAGCCGCGATCTGCTGGTCAACCTGGCCAATGAAGTGGGCCTGCAGGACGCCATCAAGTCGA  >  AE015451/2032204‑2032772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.