Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	AE015451	1,709,370	2640 bp→CGGCCGC		[ppc]	[ppc]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	AE015451	1709370	1712009	2640	38 [0]	[0] 38	[ppc]	[ppc]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 1709369	0 (0.000)	38 (0.550) +CGGCCGC	38/586	0.6	100%	intergenic (‑136/‑13)	PP_1504/ppc	conserved protein of unknown function/Phosphoenolpyruvate carboxylase
	?	AE015451	1712010 =	0 (0.000)					intergenic (+1/‑257)	ppc/adk	Phosphoenolpyruvate carboxylase/Adenylate kinase

CGATCAGCGCGGCCAGGCGCGCTTCGGACTCCAGGTCCAGGTGCAGGACATGACCGGTACGGCTGAGGGTACCGGCGGCATTCTGTTCCCAGTGCTGGGCGAGTTCACGGATTTTCATGATGACCTCTGTTGACGCCTGGAAAGGCTGCACTGCATGGGAGTGCTGTGCTTTCACTTTAGTTTGGTTTGGCCGATCACGGCTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCGATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGACCGTCGCGGCCCGGGTGAGCAGCTGAGCTCGACCCTGGCGGACCTGGCCGAAGAAGACCTGCTGCCCGTAGCGAGGGCCTTCAACCAGTTCCTCAACCTGGCCAACATGGCCGAGCAGTACCAGTTGATCCGCCGCCGTGACGCCGA  >  AE015451/1709083‑1709662                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                      cGATCAGCGCGGCCAGGCGCGCTTCGGACTCCAGGTCCAGGTGCAGGACATGACCGGTACGGCTGAGGGTACCGGCGGCATTCTGTTCCCAGTGCTGGGCGAGTTCACGGATTTTCATGATGACCTCTGTTGACGCCTGGAAAGGCTGCACTGCATGGGAGTGCTGTGCTTTCACTTTAGTTTGGTTTGGCCGATCACGGCTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaag                                                                                                                                                                                                                                                                                         >  3:482569‑M1/1‑287 (MQ=255)   aTCAGCGCGGCCAGGCGCGCTTCGGACTCCAGGTCCAGGTGCAGGACATGACCGGTACGGCTGAGGGTACCGGCGGCATTCTGTTCCCAGTGCTGGGCGAGTTCACGGATTTTCATGATGACCTCTGTTGACGCCTGGAAAGGCTGCACTGCATGGGAGTGCTGTGCTTTCACTTTAGTTTGGTTTGGCCGATCACGGCTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcc                                                                                                                                                                                                                                                                                       <  1:482567‑M1/301‑17 (MQ=255)     cAGCGCGGCCAGGCGCGCTTCGGACTCCAGGTCCAGGTGCAGGACATGACCGGTACGGCTGAGGGTACCGGCGGCATTCTGTTCCCAGTGCTGGGCGAGTTCACGGATTTTCATGATGACCTCTGTTGACGCCTGGAAAGGCTGCACTGCATGGGAGTGCTGTGCTTTCACTTTAGTTTGGTTTGGCCGATCACGGCTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccg                                                                                                                                                                                                                                                                                     <  2:423032‑M1/301‑19 (MQ=255)        gggggccaGGCGCGCGTCGGATTCAAGGTCAAGGGGAAGGACAGGACAGGTACGGCTGAGGGTACCGGCGGCATTCTGTTCCCAGGGCTGGGCGAGTTCACGGATTTTCATGATGACCTCTGTTGACGCCTGGAAAGGCTGCACTGCATGGGAGTGCTGTGCTTTCACTTTAGTTTGGTTTGGCCGATCACGGCTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggt                                                                                                                                                                                                                                                                                  <  2:391907‑M1/298‑22 (MQ=255)                     gcgtgtcgggttgggtccaggggcaggacaggaccGGTACGGCTGAGGGTACCGGCGGGATTCTGTGCCCAGTGCTGGGCGAGTTCACGGATTTTCATGATGACCTCTGTTGACGCCTGGAAAGGCTGCACTGCATGGGAGTGCTGTGCTTTCACTTTAGTTTGGTTTGGCCGATCACGGCTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggtgac                                                                                                                                                                                                                                                                     <  2:290651‑M1/288‑35 (MQ=255)                             cTCCAGGTCCAGGTGCAGGACATGACCGGTACGGCTGAGGGTACCGGCGGCATTCTGTTCCCAGTGCTGGGCGAGTTCACGGATTTTCATGATGACCTCTGTTGACGCCTGGAAAGGCTGCACTGCATGGGAGTGCTGTGCTTTCACTTTAGTTTGGTTTGGCCGATCACGGCTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGAGACCGCTCGCTCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggtgacggccataa                                                                                                                                                                                                                                                             >  2:504451‑M1/1‑259 (MQ=255)                                  ggtccaggtGCAGGACATGACCGGTACGGCTGAGGGTACCGGCGGCATTCTGTTCCCAGTGCTGGGCGAGTTCACGGATTTTCATGATGACCTCTGTTGACGCCTGGAAAGGCTGCACTGCATGGGAGTGCTGTGCTTTCACTTTAGTTTGGTTTGGCCGATCACGGCTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGCGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggtgacagatcggaagatc                                                                                                                                                                                                                                                        >  4:290719‑M1/1‑254 (MQ=255)                                           tcaGGACATGACCGGTACGGCTGAGGGTACCGGCGGCATTCTGTTCCCAGTGCTGGGCGAGTTCACGGATTTTCATGATTACCTCTGTTGACGCCTGGAAAGGCTGCACTGCATGGGAGTGCTGTGCTTTCACTTTAGTTTGGTTTGGCCGATCACGGCTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggtgacggccatacaggcccggcacaag                                                                                                                                                                                                                                               <  4:504593‑M1/300‑57 (MQ=255)                                               gACATGACCGGTACGGCTGAGGGTACCGGCGGCATTCTGTTCCCAGTGCTGGGCGAGTTCACGGATTTTCATGATGACCTCTGTTGACGCCTGGAAAGGCTGCACTGCATGGGAGTGCTGTGCTTTCACTTTAGTTTGGTTTGGCCGATCACGGCTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggtgacggccatacaggcccggcacaaggacg                                                                                                                                                                                                                                           >  3:67241‑M1/1‑241 (MQ=255)                                                    gagcggtACGGGTGAGGGTATCGGCGGCATGCTGTTCCCAGGGCTGGGCGAGTTCTAGGATGTTCATGATGACCTCTGTTGACGGCTGGAAAGGCTGCACTGCATGGGAGTGCTGTGCTTTCACTTTAGTTTGGTTTGGCCGATCACGGCTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggtgacggccatacaggcccggcacaaggacggggag                                                                                                                                                                                                                                      <  4:598398‑M1/298‑66 (MQ=255)                                                    gACCGGTACGGCTGAGGGTACCGGCGGCATTCTGTTCCCAGTGCTGGGCGAGTTCACGGATTTTCATGATGACCTCTGTTGACGCCTGGAAAGGCTGCACTGCATGGGAGTGCTGTGCTTTCACTTTAGTTTGGTTTGGCCGATCACGGCTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggttacggccatacaggcccggcacaaggacggggag                                                                                                                                                                                                                                      >  3:25765‑M1/1‑236 (MQ=255)                                                          tACGGCTGAGGGTACCGGCGGCATTCTGTTCCCAGTGCTGGGCGAGTTCACGGATTTTCATGATGACCTCTGTTGACGCCTGGAAAGGCTGCACTGCATGGGAGTGCTGTGCTTTCACTTTAGTTTGGTTTGGCCGATCACGGCTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatttctggtgacggccatacaggccctgccccaggacggggacggcggg                                                                                                                                                                                                                                >  2:10457‑M1/1‑230 (MQ=255)                                                          tACGGCTGAGGGTACCGGCGGCATTCTGTTCCCAGTGCTGGGCGAGTTCACGGATTTTCATGATGACCTCTGTTGACGCCTGGAAAGGCTGCACTGCATGGGAGTGCTGTGCTTTCACTTTAGTTTGGTTTGGCCGATCACGGCTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggtgacggccatacaggcccggcacaaggacggggagggcggt                                                                                                                                                                                                                                <  3:254971‑M1/301‑72 (MQ=255)                                                           aCGGCTGAGGGTACCGGCGGCATTCTGTTCCCAGTGCTGGGCGAGTTCACGGATTTTCATGATGACCTCTGTTGACGCCTGGAAAGGCTGCACTGCATGGGAGTGCTGTGCTTTCACTTTAGTTTGGTTTGGCCGATCACGGCTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggtgacggccatacaggcccggcacaaggacggggagggcggtt                                                                                                                                                                                                                               <  1:67239‑M1/301‑73 (MQ=255)                                                                                                              gATTTTCATGATGACCTCTGTTGACGCCTGGAAAGGCTGGATTGAAGGGGAGTGCTGTGTTTTCACTTTAGTTTGGTTTGGCCGATCACGGCTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggtgacggccatacaggcccggcacaaggacggggagggcggttgcgccaggcgcaaccgcctgcccggtcagccgcaagtggcgcattcctgca                                                                                                                                                                            <  4:10457‑M1/301‑124 (MQ=255)                                                                                                                   tCATGATGACCTCTGTTGACGCCTGGAAAGGCTGCACTGCATGGGAGTGCTGTGCTTTCACTTTAGTTTGGTTTGGCCGATCACGGCTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggtgacggccatacaggcccggcacaaggacggggagggcggttgcgccaggcgcaaccgcctgcccggtcagccgcaagtggcgcattcctgcaacttt                                                                                                                                                                       >  3:625064‑M1/1‑173 (MQ=255)                                                                                                                                tGTTGACGCCTGGAAAGGCTGCACTGCATGGGTGTGCTGTGCTTTCACTTTAGTTTGGTTTGGCCGATCACGGCTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggtgacggccatacaggcccggcacaaggacggggagggcggttgcgccaggcgcaaccgcctgcccggtcagccgcaagtggcgcattcctgcaactttgggacgcttgtct                                                                                                                                                          <  4:203739‑M1/301‑142 (MQ=255)                                                                                                                                    gACGCCTGGAAAGGCTGCACTGCATGGGAGTGCTGTGCTTTCACTTTAGTTTGGTTTGGCCGATCACGGCTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggtgacggccatacaggcccggcacaaggacggggagggcggttgcgccaggcgcaaccgcctgcccggtcagccgcaagtggcgcattcctgcaactttgggacgcttgtctggct                                                                                                                                                      <  1:625062‑M1/301‑146 (MQ=255)                                                                                                                                        ccTGGAAAGGCTGCACTGCATGGGAGTGCTGTGCTTTCACTTTAGTTTGGTTTGGCCGATCACGGCTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggtgacggccatacaggcccggcacaaggacggggagggcggttgcgccaggcgcaaccgcctgcccggtcagccgcaagtggcgcattcctgcaactttgggacgcttgtctggctgccg                                                                                                                                                  >  3:244273‑M1/1‑152 (MQ=255)                                                                                                                                                               gAGTGCTGTGCTGTCACTTTAGTTTGGTTTGGCCGATCACGGCTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGTGCCGGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggtgacggccatacaggcccggcacaaggacggggagggcggttgcgccaggcgcaaccgcctgcccggtcagccgcaagtggcgcattcctgcaactttgggacgcttgtctggctgccgggcgctgtgtatcttgagcagcc                                                                                                                           <  1:25765‑M1/301‑173 (MQ=255)                                                                                                                                                                        ctcttccgatctgtttggtttggccgatcacggcTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggtgacggccatacaggcccggcacaaggacggggagggcggttgcgccaggcgcaaccgcctgcccggtcagccgcaagtggcgcattcctgcaactttgggacgcttgtctggctgccgggcgctgtgtatcttgagcagccttctgaccg                                                                                                                  <  1:11115‑M1/289‑182 (MQ=255)                                                                                                                                                                         ctTTCACTTTAGTTTGGTTTGGCCGATCACGGCTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggtgacggccatacaggcccggcacaaggacggggagggcggttgcgccaggcgcaaccgcctgcccggtcagccgcaagtggcgcattcctgcaactttgggacgcttgtctggctgccgggcgctgtgtatcttgagcagccttctgaccga                                                                                                                 >  4:340825‑M1/1‑119 (MQ=255)                                                                                                                                                                             cACTTTAGTTTGGTTTGGCCGATCACGGCTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggtgacggccatacaggcccggcacaaggacggggagggcggttgcgccaggcgcaaccgcctgcccggtcagccgcaagtggcgcattcctgcaactttgggacgcttgtctggctgccgggcgctgtgtatcttgagcagccttctgaccgatttt                                                                                                             >  3:41353‑M1/1‑115 (MQ=255)                                                                                                                                                                                    gtttggtttgGCCGATCACGGCTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggtgacggccatacaggcccggcacaaggacggggagggcggttgcgccaggcgcaaccgcctgcccggtcagccgcaagtggcgcattcctgcaactttgggacgcttgtctggctgccgggcgctgtgtatcttgagcagccttctgaccgagatcggaagag                                                                                                      >  3:11115‑M1/1‑108 (MQ=255)                                                                                                                                                                                        ggtttggCCGATCACGGTGGGCCTTTGGCGCTGGGGTGTGGGCACTCTTGGGGGATACCGCTCGCTCATGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggtgacggccatacaggcccggcacaaggacggggagggcggttgcgccaggcgcaaccgcctgcccggtcagccgcaagtggcgcattcctgcaactttgggacgcttgtctggctgccgggcgctgtgtatcttgagcagccttctgaccgatttatggtcataccc                                                                                                  <  2:340746‑M1/301‑198 (MQ=255)                                                                                                                                                                                         gtttggCCGATCACGGCTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggtgacggccatacaggcccggcacaaggacggggagggcggttgcgccaggcgcaaccgcctgcccggtcagccgcaagtggcgcattcctgcaactttgggacgcttgtctggctgccgggcgctgtgtatcttgagcagccttctgaccgatttatggtcatacccc                                                                                                 >  3:580929‑M1/1‑103 (MQ=255)                                                                                                                                                                                             ggCCGATCACGGCTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggtgacggccatacaggcccggcacaaggacggggagggcggttgcgccaggcgcaaccgcctgcccggtcagccgcaagtggcgcattcctgcaactttgggacgcttgtctggctgccgggcgctgtgtatcttgagcagccttctgaccgatttatggtcatacccgattt                                                                                             >  3:420228‑M1/1‑99 (MQ=255)                                                                                                                                                                                                cGATCACGGCTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGCGCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggtgacggccatacaggcccggcacaaggacggggagggcggttgcgccaggcgcaaccgcctgcccggtcagccgcaagtggcgcattcctgcaactttgggacgcttgtctggctgccgggcgctgtgtatcttgagcagccttctgaccgatttatggtcatacccgattttcc                                                                                          <  1:580927‑M1/301‑206 (MQ=255)                                                                                                                                                                                                      cGGCTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggtgacggccatacaggcccggcacaaggacggggagggcggttgcgccaggcgcaaccgcctgcccggtcagccgcaagtggcgcattcctgcaactttgggacgcttgtctggctgccgggcgctgtgtatcttgagcagccttctgaccgatttatggtcatacccgatttttcggtctt                                                                                    >  4:780179‑M1/1‑90 (MQ=255)                                                                                                                                                                                                           tGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggtgacggccatacaggcccggcacaaggacggggagggcggttgcgccaggcgcaaccgcctgcccggtcagccgcaagtggcgcattcctgcaactttgggacgcttgtctggctgccgggcgctgtgtatcttgagcagccttctgaccgatttatggtcatacccgattttccggacttggccc                                                                               >  3:345175‑M1/1‑85 (MQ=255)                                                                                                                                                                                                                                gcgacactggggggataccgtgcGCTCGTGCACAAGGCGCGCT‑AGGACTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggtgacggccatccaggcccggcacaaggacggggagggcggttgcgccaggcgcaaccgcctgcccggtcagccgcaagtggcgcattcctgcaactttgggacgcttgtctggctgccgggcgctgtgtatcttgagcagccttctgaccgatttatggtcatacccgattttccggacttggccctggtcgccgaatccattgaat                                                          <  2:779899‑M1/292‑238 (MQ=255)                                                                                                                                                                                                                                          ggggATACCGCTCGCTCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggtgacggccatacaggcccggcacaaggacggggagggcggttgcgccaggcgcaaccgcctgcccggtcagccgcaagtggcgcattcctgcaactttgggacgcttgtctggctgccgggcgctgtgtatcttgagcagccttctgaccgatttatggtcatacccgattttccggacttggccctggtcgccgaatccattgaatttcatacaaa                                                >  4:783584‑M1/1‑54 (MQ=255)                                                                                                                                                                                                                                                       ctgcggatctagggcgggctgAGG‑CTGATAGGGATGAAACGcggccgcgggcaagcccgggtcatgtctggtgacggccatacaggcccggcacaaggacggggagggcggttgcgccaggcgcaaccgcctgcccggtcagccgcaagtggcgcattcctgcaactttgggacgcttgtctggctgccgggcgctgtgtatcttgagcagccttctgaccgatttatggtcatacccgattttccggacttggccctggtcgccgaatccattgaatttcatacaaaaatttgaggagca                                   <  2:395977‑M1/291‑261 (MQ=255)                                                                                                                                                                                                                                                                 aGGGCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggtgacggccatacaggcccggcacaaggacggggagggcggttgcgccaggcgcaaccgcctgcccggtcagccgcaagtggcgcattcctgcaactttgggacgcttgtctggctgccgggcgctgtgtatcttgagcagccttctgaccgatttatggtcatacccgattttccggacttggccctggtcgccgaatccattgaatttcatacaaaaatttgaggagcaagatcggaag                         >  4:396074‑M1/1‑31 (MQ=255)                                                                                                                                                                                                                                                                   ggCGCGCTGAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggtgacggccatacaggcccggcacaaggacggggagggcggttgcgccaggcgcaaccgcctgcccggtcagccgcaagtggcgcattcctgcaactttgggacgcttgtctggctgccgggcgctgtgtatcttgagcagccttctgaccgatttatggtcatacccgattttccggacttggccctggtcgccgaatccattgaatttcatacaaaaatttgaggagcacaagatgcgcgt                       <  1:41352‑M1/301‑273 (MQ=255)                                                                                                                                                                                                                                                                          ggaGG‑CTGATAGGGAGGCAACGcggccgcgggcaagcccgggtcatgtctggtgacggccatacaggcccggcacaaggacggggagggcggttgcgccaggcgcaaccgcctgcccggtcagccgcaagtggcgcattcctgcaactttgggacgcttgtctggctgccgggcgctgtgtatcttgagcagccttctgaccgatttatggtcatacccgattttccggacttggccctggtcgccgaatccattgaatttcatacaaaaatttgaggagcacaagatgcgcgtaattctg                <  2:783304‑M1/300‑280 (MQ=255)                                                                                                                                                                                                                                                                           gAGG‑CTGATAGGGATGCAACGcggccgcgggcaagcccgggtcatgtctggtgacggccatacaggcccggcacaaggacggggagggcggttgcgccaggcgcaaccgcctgcccggtcagccgcaagtggcgcattcctgcaactttgggacgcttgtctggctgccgggcgctgtgtatcttgagcagccttctgaccgatttatggtcatacccgattttccggacttggccctggtcgccgaatccatgtaatttaatacaaaaatttgaggagcacaaggtgcgggtaattcagg               >  2:264934‑M1/1‑21 (MQ=255)                                                                                                                                                                                                                                                                                         gtgCAACGcggccgcgggcaagcccgggtcatgtctggtgacggccatacaggcccggcacaaggacggggagggcggttgcgccaggcgcaaccgcctgcccggtcagccgcaagtggcgcattcctgcaactttgggacgcttgtctggctgccgggcgctgtgtatcttgagcagccttctgaccgatttatggtcatacccgattttccggacttggccctggtcgccgaatccattgaatttcatacaaaaatttgaggagcacaagatgcgcgtaattctgctgggagctcccgg  <  4:265000‑M1/300‑294 (MQ=255)                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                      CGATCAGCGCGGCCAGGCGCGCTTCGGACTCCAGGTCCAGGTGCAGGACATGACCGGTACGGCTGAGGGTACCGGCGGCATTCTGTTCCCAGTGCTGGGCGAGTTCACGGATTTTCATGATGACCTCTGTTGACGCCTGGAAAGGCTGCACTGCATGGGAGTGCTGTGCTTTCACTTTAGTTTGGTTTGGCCGATCACGGCTTGCCATCGTCGCTCGGCTGTGGGCACTCTTGGGGGATACCGCTCGCTCCTGCACAGGGCGCGCTGAGG‑CTGATAGGGATGCAACGGAGAGAGCGCCAATGACCGATATCGATGTGCGTTTGCGTGAAGATGTCCATGTGTTGGGTGAACTGCTTGGCGAAACCATTCGCCAACAGCATGGCGATGCGTTCCTGCAGAAAATCGAGGACATCCGCCACAGTGCCAAGGCCGACCGTCGCGGCCCGGGTGAGCAGCTGAGCTCGACCCTGGCGGACCTGGCCGAAGAAGACCTGCTGCCCGTAGCGAGGGCCTTCAACCAGTTCCTCAACCTGGCCAACATGGCCGAGCAGTACCAGTTGATCCGCCGCCGTGACGCCGA  >  AE015451/1709083‑1709662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑