Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	AE015451	1,164,572	830 bp→TCA	coding (4‑833/864 nt)	hexR ←	DNA‑binding transcriptional regulator

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	AE015451	1164572	1165401	830	57 [0]	[0] 58	hexR	DNA‑binding transcriptional regulator

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 1164571	0 (0.000)	57 (0.820) +TCA	51/594	0.2	100%	coding (834/864 nt)	hexR	DNA‑binding transcriptional regulator
	?	AE015451	1165402 =	0 (0.000)					coding (3/864 nt)	hexR	DNA‑binding transcriptional regulator

GGCGAAGATCTTCTGGGTGTAGGCGGCCGGGTCATCGTCGGCCTCTACCGCGCGGCTGACATAGGCGGCACCACTGGCCAGGCTCTGCGCCAGTTGCAGCTTGAGTTCGGGGTAGCCGCTGACGCCGAACGAGCGGCAGAAGCGGTTGACGGTCGGTTCGCTGACCTTGGCCGCCTGGGCCAGCGCAGCGATGCTGAAACGGGTGGCTTGTTGCGGGTTGAGCAGGATGACTTCGGCGACTTTGCGTTCGGCCTTGTTCAGCTCGTCGAGGCGGCCCTGGATCTGTTCCAGGAGGTTTCGCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGGCGCCCTCGGCGACCTGGCATTGCGCAAGCTGTTTCCTGCGCT  >  AE015451/1165102‑1165701                                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                            gcgtaagctcaagggcggctgccagaaggtctaccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcac                                                                                                                                                                                                                                                                                                             <  1:187797‑M2/1‑1 (MQ=255)        ctcaagggcggctgccagaaggtctaccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGt                                                                                                                                                                                                                                                                                                      >  3:258265‑M2/294‑301 (MQ=255)           aagggcggctgccagaaggtctaccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGtccc                                                                                                                                                                                                                                                                                                   >  1:124438‑M2/291‑300 (MQ=255)              ggcggctgccagaaggtctaccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATgg                                                                                                                                                                                                                                                                                                <  1:35485‑M2/14‑1 (MQ=255)                gggcggccagaaggtctaccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGgt                                                                                                                                                                                                                                                                                              <  2:93243‑M2/16‑1 (MQ=255)                       ggttctggctaccccggtgcgccacgggggcaatcgcaggaccgggcctggcagcgcgagctgtgcatcgataccggtgacggcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCt                                                                                                                                                                                                                                                                                       <  2:377867‑M2/23‑1 (MQ=255)                             gtctaccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTgggtcctaggtcg                                                                                                                                                                                                                                                                                 >  4:377957‑M2/273‑295 (MQ=255)                              acgtgtgctcttccgatctggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCg                                                                                                                                                                                                                                                                                <  3:356116‑M2/30‑1 (MQ=255)                                gatcccggtacgcaacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCggg                                                                                                                                                                                                                                                                              <  3:167427‑M2/32‑1 (MQ=255)                                     cggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTcgggagatc                                                                                                                                                                                                                                                                         >  1:167425‑M2/265‑297 (MQ=255)                                             cacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCt                                                                                                                                                                                                                                                                 >  1:461084‑M2/257‑301 (MQ=255)                                               cgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCtgt                                                                                                                                                                                                                                                               <  4:388184‑M2/47‑1 (MQ=255)                                                 ggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCatgggtgtgtccttgggtcgagatcggaagagcgtcgtg                                                                                                                                                                                                                                                             >  1:356115‑M2/253‑282 (MQ=255)                                                       attgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGagcag                                                                                                                                                                                                                                                       <  3:124440‑M2/55‑1 (MQ=255)                                                                cccggccgggcggcggggacggtggaacgataccggggacagcgacgagaccttggtctggcaccctggcaatcgcccgcggatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCtttt                                                                                                                                                                                                                                              <  2:394432‑M2/64‑1 (MQ=255)                                                                  cgatctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTg                                                                                                                                                                                                                                            <  1:149645‑M2/66‑1 (MQ=255)                                                                        ggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGctttttgagatcg                                                                                                                                                                                                                                      >  3:149647‑M2/230‑295 (MQ=255)                                                                                  gctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGt                                                                                                                                                                                                                            >  1:341592‑M2/220‑301 (MQ=255)                                                                                                       cagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTa                                                                                                                                                                                                       <  1:258266‑M2/103‑1 (MQ=255)                                                                                                             cgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGcagcaggctttttgcacggtcgtctatcgagatcggaagagcacacgtctgaactcc                                                                                                                                                                                                 >  4:552419‑M2/193‑273 (MQ=255)                                                                                                                  ccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTg                                                                                                                                                                                            >  3:410937‑M2/188‑301 (MQ=255)                                                                                                                        tctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAacac                                                                                                                                                                                      <  4:419206‑M2/120‑1 (MQ=255)                                                                                                                          tggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACtt                                                                                                                                                                                    >  3:169055‑M2/180‑301 (MQ=255)                                                                                                                                   ggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAAt                                                                                                                                                                           >  3:359493‑M2/171‑301 (MQ=255)                                                                                                                                    gcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAAtg                                                                                                                                                                          <  1:359492‑M2/132‑1 (MQ=255)                                                                                                                                     caatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAAtgt                                                                                                                                                                         <  3:461086‑M2/133‑1 (MQ=255)                                                                                                                                       atcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGCAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACGTGCCTGTAACACTTGTGTGtagtgtgt                                                                                                                                                                       >  2:587576‑M2/167‑299 (MQ=255)                                                                                                                                              gctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGtttt                                                                                                                                                                >  3:84453‑M2/160‑301 (MQ=255)                                                                                                                                               gtgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGttttt                                                                                                                                                               <  1:410935‑M2/143‑1 (MQ=255)                                                                                                                                                      gggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACAt                                                                                                                                                        <  1:84450‑M2/150‑1 (MQ=255)                                                                                                                                                                   cgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGaaaa                                                                                                                                           >  4:210498‑M2/139‑301 (MQ=255)                                                                                                                                                                        cgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCgtg                                                                                                                                      >  1:225759‑M2/134‑301 (MQ=255)                                                                                                                                                                           agcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAAccgtgttg                                                                                                                                   >  4:106111‑M2/131‑299 (MQ=255)                                                                                                                                                                           agcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACcgtgtgg                                                                                                                                   >  4:309737‑M2/131‑300 (MQ=255)                                                                                                                                                                                    tctttccctacccgacgctcttccgatctgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGt                                                                                                                          <  1:594186‑M2/180‑1 (MQ=255)                                                                                                                                                                                     ctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTa                                                                                                                         >  4:172839‑M2/121‑301 (MQ=255)                                                                                                                                                                                         gtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTcc                                                                                                                     >  4:696358‑M2/117‑301 (MQ=255)                                                                                                                                                                                           ggaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTa                                                                                                                   <  3:225757‑M2/187‑1 (MQ=255)                                                                                                                                                                                            gaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAg                                                                                                                  <  4:587761‑M2/188‑1 (MQ=255)                                                                                                                                                                                             gggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGa                                                                                                                 <  3:341593‑M2/189‑1 (MQ=255)                                                                                                                                                                                                ggaccagtggcaggggcgaaggcctgagcctgggacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCa                                                                                                              <  2:210448‑M2/192‑1 (MQ=255)                                                                                                                                                                                                                 gaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTAtcccttgaaaaccgtgtgtgaaagcggtagatcggaagagcacacgtctgaactcca                                                                                             >  3:594188‑M2/93‑273 (MQ=255)                                                                                                                                                                                                                         ggggctgggaccggggcggcgggggcacctgcgtttgcaggggcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATg                                                                                     <  2:106087‑M2/217‑1 (MQ=255)                                                                                                                                                                                                                                                    cctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCAcc                                                          >  3:288512‑M2/58‑301 (MQ=255)                                                                                                                                                                                                                                                     ctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCt                                                         >  1:391711‑M2/57‑301 (MQ=255)                                                                                                                                                                                                                                                      tgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCtt                                                        <  3:391711‑M2/246‑1 (MQ=255)                                                                                                                                                                                                                                                       ctacacgtggcgtttgcggtctagttgagatggtcgtcctcgctccggtaatcgCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCttt                                                       <  2:34415‑M2/247‑1 (MQ=255)                                                                                                                                                                                                                                                          tttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGcc                                                    <  3:412991‑M2/250‑1 (MQ=255)                                                                                                                                                                                                                                                          tttgcaggcgaaccggctcaggtgaggtcggcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGcc                                                    <  2:696128‑M2/250‑1 (MQ=255)                                                                                                                                                                                                                                                                ggcgcaccggctcaggtgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGttt                                              <  2:172797‑M2/256‑1 (MQ=255)                                                                                                                                                                                                                                                                         gctcagtgtaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGGCGCCCTc                                     <  2:309664‑M2/265‑1 (MQ=255)                                                                                                                                                                                                                                                                             agttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGTAAGCGGTATTCCTAGACCAACTTTATGAAAGAACCAACATCATGGCCGCaatcagtgtcgaaccttgcacctttagatcggaagagcacacgtctg                                 >  4:34426‑M2/33‑279 (MQ=255)                                                                                                                                                                                                                                                                                   ggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCCTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGCCGCCCCCGGCGACCTgg                           >  2:454188‑M2/27‑301 (MQ=255)                                                                                                                                                                                                                                                                                          gtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGGCGCCCTCGGCGACCTGGCATTgcg                    >  4:239905‑M2/20‑301 (MQ=255)                                                                                                                                                                                                                                                                                                gatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGGCGCCCTCGGCGACCTGGCATTGCGCAAGCt              <  2:239851‑M2/288‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  tctggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGGCGCCCTCGGCGACCTGGCATTGCGCAAGCTGt            <  1:456720‑M2/290‑1 (MQ=255)                                                                                                                                                                                                                                                                                                     ggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGGCGCCCTCGGCGACCTGGCATTGCGCAAGctgtaga         >  3:456722‑M2/9‑298 (MQ=255)                                                                                                                                                                                                                                                                                                            aCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGGCGCCCTCGGCGACCTGGCATTGCGCAAGCTGTTTCCTGCGCt  <  1:169053‑M2/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                            GGCGAAGATCTTCTGGGTGTAGGCGGCCGGGTCATCGTCGGCCTCTACCGCGCGGCTGACATAGGCGGCACCACTGGCCAGGCTCTGCGCCAGTTGCAGCTTGAGTTCGGGGTAGCCGCTGACGCCGAACGAGCGGCAGAAGCGGTTGACGGTCGGTTCGCTGACCTTGGCCGCCTGGGCCAGCGCAGCGATGCTGAAACGGGTGGCTTGTTGCGGGTTGAGCAGGATGACTTCGGCGACTTTGCGTTCGGCCTTGTTCAGCTCGTCGAGGCGGCCCTGGATCTGTTCCAGGAGGTTTCGCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGGCGCCCTCGGCGACCTGGCATTGCGCAAGCTGTTTCCTGCGCT  >  AE015451/1165102‑1165701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑