Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	AE015451	4,892,114	1412 bp→GCGGCCGC	coding (5‑1416/1416 nt)	pyk →	Pyruvate kinase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	AE015451	4892114	4893525	1412	40 [0]	[0] 38	pyk	Pyruvate kinase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 4892113	0 (0.000)	38 (0.560) +GCGGCCGC	33/584	0.8	100%	coding (1275/1275 nt) coding (4/1416 nt)	ttuD pyk	putative hydroxypyruvate reductase Pyruvate kinase
	?	AE015451	4893526 =	0 (0.000)					intergenic (+1/‑207)	pyk/PP_4302	Pyruvate kinase/Urea transporter

GGTCAACTACAGCGAGTCGGGTGCCTCGACCCTGCGCGCGGCACGTGAACGGCCACGGGCACCGATCCTCAACCTGACGCCGAACCTGAACACCGCGCGCCGCCTGAGCGTGGCTTGGGGTGTGCATTCGGTGGTCAACGACCGCCTGCGTCAGGTCGACGAGGTGGTTTCCACCGCCCTGGAGATTGCCCAGGCGCAAGGCATGGCCAGCCGTGGCGACACGCTGCTGATCACCGCTGGCGTGCCTTTCGGCAAGCCGGGTACGACTAACACGCTGCGGATCGAGACTTTGATCTGAGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAGGGCCACGCTGGCACAAGGGAATGCAGTCCCTGTGGGAGCGGCCTTGTGTCGCGATTGGAGGGCAAAGCCCTCCCTGAATACCGCTCTACCCGACCGCGGAAAACCGAGGCCCAAAGAGGCCCACCGCTCCCCCATCAACCTCCACTGACTGCCCCATGTACACCAAAAATTTCGTCAACCCGTGCCCCGACTGGGCCACGGCGTTACTCAACGGCTTCA  >  AE015451/4893228‑4893796                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                               tctttccctacacgacgctcttccgatctctgaaaggcctgccgggcgtgtacgccctggccggtgacaccgacggcatcgatggctcggaagaaaacgccggtgccttcatgaccccggccagctacgccagcgccgaagccttgggcctgtcggccagcgacgagctggacaacaacaacggctacggctacttcgccgcgctggatgcgctgatcgtcaccgaaccgacccgcaccaacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGAt                                                                                                                                                                                                                                                                              <  1:42065‑M2/3‑1 (MQ=255)        cctgctcagcctaaccgaaagcctgaaaggcctgccgggcgtgtacgccctggccggtgacaccgacggcatcgatggctcggaagaaaacgccggtgccttcatgaccccggccagctacgccagcgccgaagccttgggcctgtcggccagcgacgagctggacaacaacaacggctacggctacttcgccgcgctggatgcgctgatcgtcaccgaaccgacccgcaccaacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCggg                                                                                                                                                                                                                                                                       <  2:751185‑M2/10‑1 (MQ=255)                          gtttcggaaaggcctgccgggggcgtacggcctggccggtgacaccgacggcatcgatggctcggaagaaaacgccggtgccttcatgaccccggccagctacgccagcgccgaagccttgggcctgtcggccagcgacgagctggacaacaacaacggctacggctacttcgccgcgctggatgcgctgatcgtcaccgaaccgacccgcaccaacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCGCCCCttt                                                                                                                                                                                                                                                     <  2:269308‑M2/28‑1 (MQ=255)                           agcctgaaaggcctgccgggcgtgtacgccctggccggttacaccgacggcatcgatggctcggaagaaaacgccggtgccttcatgaccccggccagctacgccagcgccgaagccttgggcctgtcggccagcgacgagctggacaacaacaacggctacggctacttcgccgcgctggatgcgctgatcgtcaccgaaccgacccgcaccaacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTc                                                                                                                                                                                                                                                    <  4:530993‑M2/29‑1 (MQ=255)                           agcctgaaaggcctgccgggcgtgtacgccctggccggtgacaccgacggcatcgatggctcggaagaaaacgccggtgccttcatgaccccggccagctacgccagcgccgaagccttgggcctgtcggccagcgacgagctggacaacaacaacggctacggctacttcgccgcgctggatgcgctgatcgtcaccgaaccgacccgcaccaacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTc                                                                                                                                                                                                                                                    <  4:406255‑M2/29‑1 (MQ=255)                             cctgaaaggcctgccgggcgtgtacgccctggccggtgacaccgacggcatcgatggctcggaagaaaacgccggtgccttcatgaccccggccagctacgccagcgccgaagccttgggcctgtcggccagcgacgagctggacaacaacaacggctacggctacttcgccgcgctggatgcgctgatcgtcaccgaaccgacccgcaccaacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCCCCCCTTTcgc                                                                                                                                                                                                                                                  >  1:227973‑M2/271‑301 (MQ=255)                              ctgaaaggcctgccgggcgtgtacgccctggccggtgacaccgacggcatcgatggctcggaagaaaacgccggtgccttcatgaccccggccagctacgccagcgccgaagccttgggcctgtcggccagcgacgagctggacaacaacaacggctacggctacttcgccgcgctggatgcgctgatcgtcaccgaaccgacccgcaccaacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcgatagatcggaagagcacacgtctgaactcca                                                                                                                                                                                                                                                 >  3:42066‑M2/270‑272 (MQ=255)                              ctgaaaggcctgccgggcgtgtacgccctggccggtgacaccgacggcatcgatggctcggaagaaaacgccggtgccttcatgaccccggccagctacgccagcgccgaagccttgggcctgtcggccagcgacgagctggacaacaacaacggctacggctacttcgccgcgctggatgcgctgatcgtcaccgaaccgacccgcaccaacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCGcccctttagat                                                                                                                                                                                                                                                 >  4:269374‑M2/270‑297 (MQ=255)                                                  cttccgatctgcaggaatcgcggacgcagtcgagggctcggaagaaaacgccgatgccgtcatgacccctgccagctacgccagcgccgaagccttgggcctgtcggccagcgacgagctggacaacaacaacggctacggctacttcgccgcgctggatgcgctgatcgtcaccgaaccgacccgcaccaacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAg                                                                                                                                                                                                                             <  1:333266‑M2/52‑1 (MQ=255)                                                            gccggtgacaccgacggcatcgatggctcggaagaaaacgccggtgccttcatgaccccggccagctacgccagcgccgaagccttgggcctgtcggccagcgacgagctggacaacaacaacggctacggctacttcgccgcgctggatgcgctgatcgtcaccgaaccgacccgcaccaacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGCCACAAGCAcgctcccccagagatagggag                                                                                                                                                                                                                   >  3:333267‑M2/240‑291 (MQ=255)                                                               ggtgacaccgacggcatcgatggctcggaagaaaacgccggtgccttcatgaccccggccagctacgccagcgccgaagccttgggcctgtcggccagcgacgagctggacaacaacaacggctacggctacttcgccgcgctggatgcgctgatcgtcaccgaaccgacccgcaccaacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAGGGCCACGCTGGca                                                                                                                                                                                                                <  4:347385‑M2/65‑1 (MQ=255)                                                                                           aagaaaacgccggtgccttcatgaccccggccagctacgccagcgccgaagccttgggcctgtcggccagcgacgagctggacaacaacaacggctacggctacttcgccgcgctggatgcgctgatcgtcaccgaaccgacccgcaccaacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAGGGCCACGCTGGCACAAGGGAATGCAGTCCCTGTGGGAGCgg                                                                                                                                                                                    <  3:469393‑M2/93‑1 (MQ=255)                                                                                                    ccggtgccttcatgaccccggccagctacgccagcgccgaagccttgggcctgtcggccagcgacgagctggacaacaacaacggctacggctacttcgccgcgctggatgcgctgatcgtcaccgaaccgacccgcaccaacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAGGGCCACGCTGGCACAAGGGAATGCAGTCCCTGTGGGAGCGGCCTTGTGTc                                                                                                                                                                           >  4:58678‑M2/200‑301 (MQ=255)                                                                                                            ttcatgaccccggccagctacgccagcgccgaagccttgggcctgtcggccagcgacgagctggacaacaacaacggctacggctacttcgccgcgctggatgcgctgatcgtcaccgaaccgacccgcaccaacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAGGGCCCCGCTGGCCCAAGGGAATGCAGCCCCTGTGCGACCGGCCTGGTGTCCGGATTgg                                                                                                                                                                   >  2:221457‑M2/192‑301 (MQ=255)                                                                                                                tgaccccggccagctacgccagcgccgaagccttgggcctgtcggccagcgacgagctggacaacaacaacggctacggctacttcgccgcgctggatgcgctgatcgtcaccgaaccgacccgcaccaacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGGTGCGCCCCTTTCGCGACACAAGGCCGCCCCCCCAGGGCCACGCTGGCACAAGGGAATGCAGTCCCTGTGGGAGCGGCCTTGTGTTGCGAGTgggggg                                                                                                                                                               >  3:116948‑M2/188‑301 (MQ=255)                                                                                                                   ccccggccagctacgccagcgccgaagccttgggcctgtcggccagcgacgagctggacaacaacaacggctacggctacttcgccgcgctggatgcgctgatcgtcaccgaaccgacccgcaccaacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAGGGCCACGCTGGCACaagggaatgcagtccctgtgggagcgtcgctctgtcgagcggagggggaca                                                                                                                                                            >  3:125307‑M2/185‑276 (MQ=255)                                                                                                                     ccggccagctacgccagcgccgaagccttgggcctgtcggccagcgacgagctggacaacaacaacggctacggctacttcgccgcgctggatgcgctgatcgtcaccgaaccgacccgcaccaacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAGGGCCACGCTGGCACAAGGGAATGCAGTCCCTGTGGGAGCGGCCTTGTGTCGCGATTGGAGGGCAAAg                                                                                                                                                          <  1:116946‑M2/119‑1 (MQ=255)                                                                                                                              gttcagacgtgtgctcttccgatcttgtcggccagcgacgagctggacaacaacaacggctacggctacttcgccgcgctggatgcgctgatcgtcaccgaaccgacccgcaccaacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAGGGCCACGCTGGCACAAGGGAATGCAGTCCCTGTGGGAGCGGCCTTGTGTCGCGATTGGAGGGCAAAGCCCTCCCTg                                                                                                                                                 <  3:144774‑M2/128‑1 (MQ=255)                                                                                                                                             gccttgggcctgtcggccagcgacgagctggacaacaacaacggctacggctacttcgccgcgctggatgcgctgatcgtcaccgaaccgacccgcaccaacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAGGGCCACGCTGGCACAAGGGAATGCAGTCCCTGTGGGAGCGGCCTTGTGTCGCGATTGGAGGGCAAAGCCCTCCCTGAATACCGCTCTAccc                                                                                                                                  <  4:221507‑M2/143‑1 (MQ=255)                                                                                                                                                      ctgtcggccagcgacgagctggacaacaacaacggctacggctacttcgccgcgctggatgcgctgatcgtcaccgaaccgacccgcaccaacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAGGGCCACGCTGGCACAAGGGAATGCAGTCCCTGTGGGAGCGGCCTTGTGTCGCGATTGGAGGGCAAAGCCCTCCCTGAATACCGCTCTACCCGACCGCGGa                                                                                                                         >  4:28995‑M2/150‑301 (MQ=255)                                                                                                                                                       tgtcggccagcgacgagctggacaacaacaacggctacggctacttcgccgcgctggatgcgctgatcgtcaccgaaccgacccgcaccaacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAGGGCCACGCTGGCACAAGGGAATGCAGTCCCTGTGGGAGCGGCCTTGTGTCGCgattggagggcaaagccctccctgagatcggaagagcgtcgggtagggg                                                                                                                        >  1:144772‑M2/149‑277 (MQ=255)                                                                                                                                                        gtcggccagcgacgagctggacaacaacaacggctacggctacttcgccgcgctggatgcgctgatcgtcaccgaaccgacccgcaccaacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAGGGCCACGCTGGCACAAGGGAATGCAGTCCCTGTGGGAGCGGCCTTGTGTCGCGATTGGAGGGCAAAGCCCTCCCTGAATACCGCTCTACCCGACCGCggaca                                                                                                                       >  4:142406‑M2/148‑299 (MQ=255)                                                                                                                                                             ccagcgacgagctggacaacaacaacggctacggctacttcgccgcgctggatgcgctgatcgtcaccgaaccgacccgcaccaacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAGGGCCACGCTGGCACAAGGGAATGCAGTCCCTGTGGGAGCGGCCTTGTGTCGCGATTGGAGGGCAAAGCCCTACCTGAATACCGCTCTACCCGACCGCGGAACACCGa                                                                                                                  >  1:12874‑M2/143‑301 (MQ=255)                                                                                                                                                                        ctggacaacaacaacggctacggctacttcgccgcgctggatgcgctgatcgtcaccgaaccgacccgcaccaacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAGGGCCACGCTGGCACAAGGGAATGCAGTCCCTGTGGGAGCGGCCTTGTGTCGCGATTGGAGGGCAAAGCCCTCCCTGAATACCGCTCTACCCGACCGCGGAAAACCGAGGCCCAAagag                                                                                                       <  3:12874‑M2/170‑1 (MQ=255)                                                                                                                                                                        ctggaaaaacaacacggccacggctacttcgcggcgctggatgcgctgatcgtcaccgaaccgacccgcaccaacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAGGGCCACGCTGGCACAAGGGAATGCAGTCCCTGTGGGAGCGGCCTTGTGTCGCGATTGGAGGGCAAAGCCCTCCCTGAATACCGCTCTACCCGACCGCGGAAAACCGAGGCCCAAagag                                                                                                       <  2:58661‑M2/170‑1 (MQ=255)                                                                                                                                                                                     ccggctacggctacttcgccgcgctggatgcgctgatcgtcaccgaaccgacccgcaccaacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAGGGCCACGCTGGCACAAGGGAATGCAGTCCCTGTGGGAGCGGCCTTGTGTCGCGATTGGAGGGCAAAGCCCTCCCTGAATACCGCTCTACCCGACCGCGGAAAACCGAGGCCCAAAGAGGCCCACCGCTccc                                                                                          <  3:227971‑M2/183‑1 (MQ=255)                                                                                                                                                                                                                       gatcgtcaccgaaccgacccgcaccaacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAGGGCCACGCTGGCACAAGGGAATGCAGTCCCTGTGGGAGCGGCCTTGTGTCGCGATTGGAGGGCAAAGCCCTCCCTGAATACCGCTCTACCCGACCGCGGAAAACCGAGGCCCAAAGAGGCCCACCGCTCCCCCATCAACCTCCACTGACTGCCCCATGTACaccc                                                        >  1:359765‑M2/85‑300 (MQ=255)                                                                                                                                                                                                                             caccgaaccgacccgcaccaacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAGGGCCACGCTGGCACAAGGGAATGCAGTCCCTGTGGGAGCGGCCTTGTGTCGCGATTGGAGGGCAAAGCCCTCCCTGAATACCGCTCTACCCGACCGCGGAAAACCGAGGCCCAAAGAGGCCCACCGCTCCCCCATCAACCTCCACTGACTGCCCCATGTACACCAAAAAtt                                                  <  3:359766‑M2/223‑1 (MQ=255)                                                                                                                                                                                                                                cgaaccgacccgcaccaacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAGGGCCACGCTGGCACAAGGGAATGCAGTCCCTGTGGGAGCGGCCTTGTGTCGCGATTGGAGGGCAAAGCCCTCCCTGAATACCGCTCTACCCGACCGCGGAAAACCGAGGCCCAAAGAGGCCCACCGCCACCCCATCAACCTCCACTGACTGCCCCATGTACACCAAAAATTtct                                               >  3:280800‑M2/76‑300 (MQ=255)                                                                                                                                                                                                                                 aacaccacccacgacaagctcaacgacctccgcgcaatcctgatccttgagacggccagaacacgagcggccgcGCTTGCCGGGGGCGCGTTGCTCCCCTTTCGCGACACAAGGCCGCTCCCACAGGGCCACGCTGGCACAAGGGAATGCAGTCCCTGTGGGAGCGGCCTTGTGTCGCGATTGGAGGGCAAAGCCCTCCCTGAATACCGCTCTACCCGACCGCGGAAAACCGAGGCCCAAAGAGGCCCACCGCTCCCCCATCAACCTCCACTGACTGCCCCATGTACACCAAAAATTTCGt                                              <  2:142377‑M2/227‑1 (MQ=255)                                                                                                                                                                                                                                        ccgcaaccaactgcaccgacttccgcgccatcctgttccttgatactgcccaagcatcggcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAGGGCCACGCTGGCACAAGGGAATGCAGTCCCTGTGGGAGCGGCCTTGTGTCGCGATTGGAGGGCAAAGCCCTCCCTGAATACCGCTCTACCCGACCGCGGAAAACCGAGGCCCAAAGAGGCCCACCGCTCCCCCATCAACCTCCACTGACTGCCCCATGTACACCAAAAATTTCGTCAACCCg                                       <  1:280801‑M2/234‑1 (MQ=255)                                                                                                                                                                                                                                          cgcaccaacgtcaacgacttcggggcaatcctgatcctggagactgcccaatcatgggcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAGGGCCACGCTGGCACAAGGGAATGCAGTCCCTGTGGGAGCGGCCTTGTGTCGCGATTGGAGGGCAAAGCCCTCCCTGAATACCGCTCTACCCGACCGCGGAAAACCGAGGCCCAAAGAGGCCCACCGCTCCCCCATCAACCTCCACTGACTGCCCCATGTACACCAAAAATTTCGTCAACCCGTg                                     <  2:28985‑M2/236‑1 (MQ=255)                                                                                                                                                                                                                                              ccgcgcttttccgacctcgcgccagccttctccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAGGGCCACGCTGGCACAAGGGAATGCAGTCCCTGTGGGAGCGGCCTTGTGTCGCGATTGGAGGGCAAAGCCCTCCCTGAATACCGCTCTACCCGACCGCGGAAAACCGAGGCCCAAAGAGGCCCACCGCTCCCCCATCAACCTCCACTGACTGCCCCATGTACACCAAAAATTTCGTCAACCCGTGcccc                                 <  2:748169‑M2/240‑1 (MQ=255)                                                                                                                                                                                                                                                aacgtcaacgacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAGGGCCACGCTGGCACAAGGGAATGCAGTCCCTGTGGGAGCGGCCTTGTGTCGCGATTGGAGGGCAAAGCCCTCCCTGAATACCGCTCTACCCGACCGCGGAAAACCGAGGCCCAAAGAGGCCCACCGCTCCCCCATCAACCTCCACTGACTGCCCCATGTACACCAAAAATTTCGTCAACCCGTGCCCcgc                               >  3:218115‑M2/60‑300 (MQ=255)                                                                                                                                                                                                                                                         gacttccgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAGGGCCACGCTGGCACAAGGGAATGCAGTCCCTGTGGGAGCGGCCTTGTGTCGCGATTGGAGGGCAAAGCCCTCCCTGAATACCGCTCTACCCGACCGCGGAAAACCGAGGCCCAAAGAGGCCCACCGCACCCCCATCAACCTCCACTGCCTGCCCCATGTACACCAAAAATTTCGTCAACCCGTGCCCCGACCGGGCCAc                      >  1:157565‑M2/51‑301 (MQ=255)                                                                                                                                                                                                                                                               cgcgccatcctgatccttgagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAGGGCCACGCTGGCACAAGGGAATGCAGTCCCTGTGGGAGCGGCCTTGTGTCGCGATTGGAGGGCAAAGCCCTCCCTGAATACCGCTCTACCCGACCGCGGAAAACCGAGGCCCAAAGAGGCCCACCGCTCCCCCATCAACCTCCACTGACTGCCCCATGTACACCAAAaatttcgtcaacccgtgccccagatcggaagagcacac                >  4:748448‑M2/45‑284 (MQ=255)                                                                                                                                                                                                                                                                        ctgatccttaagactgcccaatcatgagcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAGGGCCACGCTGGCACAAGGGAATGCAGTCCCTGTGGGAGCGGCCTTGTGTCGCGATTGGAGGGCAAAGCCCTCCCTGAATACCGCTCTACCCGACCGCGGAAAACCGAGGCCCAAAGAGGCCCACCGCTCCCCCATCAACCTCCACTGACTGTCCCATGTACACCAAAAATTTCGTCAACCCGTGCCCCGACTGGGCCACGGCGTTACTCAACgg       >  3:98681‑M2/36‑301 (MQ=255)                                                                                                                                                                                                                                                                             ccttgagactgcccaatcatgggcggccgcGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAGGGCCACGCTGGCACAAGGGAATGCAGTCCCTGTGGGAGCGGCCTTGTGTCGCGATTGGAGGGCAAAGCCCTCCCTGAATACCGCTCTACCCGACCGCGGAAAACCGAGGCCCAAAGAGGCCCACCGCTCCCCCATCAACCTCCACTGACTGCCCCATGTACACCAAAAATTTCGTCAACCCGTGCCCCGACTGGGCCACGGCGTTACTCAACGGCTTCa  <  3:157567‑M2/271‑1 (MQ=255)                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                               GGTCAACTACAGCGAGTCGGGTGCCTCGACCCTGCGCGCGGCACGTGAACGGCCACGGGCACCGATCCTCAACCTGACGCCGAACCTGAACACCGCGCGCCGCCTGAGCGTGGCTTGGGGTGTGCATTCGGTGGTCAACGACCGCCTGCGTCAGGTCGACGAGGTGGTTTCCACCGCCCTGGAGATTGCCCAGGCGCAAGGCATGGCCAGCCGTGGCGACACGCTGCTGATCACCGCTGGCGTGCCTTTCGGCAAGCCGGGTACGACTAACACGCTGCGGATCGAGACTTTGATCTGAGATTGCCGGGGGCGCGTTGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAGGGCCACGCTGGCACAAGGGAATGCAGTCCCTGTGGGAGCGGCCTTGTGTCGCGATTGGAGGGCAAAGCCCTCCCTGAATACCGCTCTACCCGACCGCGGAAAACCGAGGCCCAAAGAGGCCCACCGCTCCCCCATCAACCTCCACTGACTGCCCCATGTACACCAAAAATTTCGTCAACCCGTGCCCCGACTGGGCCACGGCGTTACTCAACGGCTTCA  >  AE015451/4893228‑4893796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑