Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	AE015451	5,342,745	1681 bp→GCGGCCGC		[pgi‑II]	[pgi‑II]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	AE015451	5342745	5344425	1681	54 [0]	[0] 52	[pgi‑II]	[pgi‑II]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 5342744	0 (0.000)	52 (0.760) +GCGGCCGC	43/584	0.4	100%	intergenic (+190/‑17)	panC/pgi‑II	pantothenate synthetase/glucose‑6‑phosphate isomerase
	?	AE015451	5344426 =	0 (0.000)					intergenic (+1/‑177)	pgi‑II/acsA‑II	glucose‑6‑phosphate isomerase/acetyl‑CoA synthetase

TCGAAAAGCTGGCCCCGCACAAGGTGATCCCGGGTAACCGCCCGAGCAACACGTTGGTGGTCGAGCGCATCAGCCCGCGCCGGCTGGGTGCGCTGGTGGCCATGTACGAGCACAAGGTGTTCGTACAGAGCGTTATCTGGGGTATCAACGCCTTCGATCAGTGGGGCGTCGAGCTGGGCAAGGAGCTGGGCAAGGGCGTTTACCAGCGCTTGGTCGGTAGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATACCCCCAGGCCCTGGCCGTCAGCCAGTCCGCCGCCCTCACCCCCGAAGACTACCGCCGCCTCTACCGCCAGTCGGTCGAAGACC  >  AE015451/5344133‑5344705                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                        atgatcgagacctggtggtgattgcggcggcttacctgggtaacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCt                                                                                                                                                                                                                                                                                  >  3:344221‑M2/294‑301 (MQ=255)   cccctttccctaaacgacgctcttccgatctacctgggtaacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTtg                                                                                                                                                                                                                                                                                <  2:611620‑M2/10‑1 (MQ=255)                tggtgattgcggcggcttacctgggtaacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTc                                                                                                                                                                                                                                                                   <  4:186510‑M2/23‑1 (MQ=255)                  gctcttccgatctcttacctgggtaacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTcgc                                                                                                                                                                                                                                                                 <  1:848‑M2/25‑1 (MQ=255)                   gtgattgcggcggcttacctgggtaacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTcgcg                                                                                                                                                                                                                                                                >  3:118460‑M2/276‑301 (MQ=255)                     gattgcggcggcttacctgggtaaaacgcggttgatcgaaaacctctacctgaatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCggg                                                                                                                                                                                                                                                              <  1:118458‑M2/28‑1 (MQ=255)                       gccggcggcttccctgggtaacacgcgggtgaccgacaacctctacctgcgtttggaagagaagaccgcataacggtcttgggctgccttgcagcccatgggcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggccttggcctataatggtgcaagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTa                                                                                                                                                                                                                                                            <  2:106198‑M2/30‑1 (MQ=255)                             ggcttacctgggtaacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcCCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAacccc                                                                                                                                                                                                                                                      >  2:50916‑M2/266‑300 (MQ=255)                               cttacctgggtaacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTgtgccggcctcttcgcagatcggaagagc                                                                                                                                                                                                                                                    >  3:848‑M2/264‑288 (MQ=255)                                        gtaacacgcggtggatcgacaacctctacctgcattgggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCa                                                                                                                                                                                                                                           <  1:344220‑M2/47‑1 (MQ=255)                                                    ttccgatctacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTc                                                                                                                                                                                                                               <  4:254515‑M2/59‑1 (MQ=255)                                                    ctggagttcagacgtgtgctcttccgatctagaagacagcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTc                                                                                                                                                                                                                               <  3:36603‑M2/59‑1 (MQ=255)                                                        cgcgaaccgctgcctgaagttggaagagaagacgtcatgacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAg                                                                                                                                                                                                                           <  2:636289‑M2/63‑1 (MQ=255)                                                         gacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAgg                                                                                                                                                                                                                          <  4:50933‑M2/64‑1 (MQ=255)                                                         gacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGAGTTACCTCGTAgg                                                                                                                                                                                                                          >  4:345641‑M2/238‑301 (MQ=255)                                                             ccctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCg                                                                                                                                                                                                                      <  3:226264‑M2/68‑1 (MQ=255)                                                             acctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACgcgttacctccgatcggag                                                                                                                                                                                                                      >  2:254457‑M2/234‑292 (MQ=255)                                                                tctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGcgggg                                                                                                                                                                                                                   >  4:191517‑M2/231‑300 (MQ=255)                                                                      tctatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTAccc                                                                                                                                                                                                             <  1:378550‑M2/77‑1 (MQ=255)                                                                         atttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTttacccaga                                                                                                                                                                                                          >  3:378550‑M2/222‑298 (MQ=255)                                                                            tggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCCCTTCGCGGGTAAACCCGCCTACCCGGCAACGCGGTACCTCGTAGGAGCGGGCTtacccgccaaa                                                                                                                                                                                                       >  3:280244‑M2/219‑297 (MQ=255)                                                                              gagttcagacgtgtgctcttccgatctgctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAagag                                                                                                                                                                                                     <  3:139486‑M2/85‑1 (MQ=255)                                                                                  agaagacagcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGtaaacccgcctaccaggccacgcgttacctcagatcggaagagcgtcgtgtagggaaagag                                                                                                                                                                                                 >  1:36602‑M2/213‑271 (MQ=255)                                                                                            ataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGgttt                                                                                                                                                                                       >  1:89139‑M2/203‑300 (MQ=255)                                                                                                         gctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTacctcgtaggagcgggtttacccgcgaagagagatcggaagcgcgtcgggtagggaaa                                                                                                                                                                          >  1:139484‑M2/190‑274 (MQ=255)                                                                                                          gggccttgacgcccattcgcgggcaagcccgtgcctgcacctttacctgtaggggctggcttgcttgcaatgcccccaaaacccccctgccctacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCGTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAg                                                                                                                                                                         <  1:280245‑M2/113‑1 (MQ=255)                                                                                                          ccgcatgcactcccagtcccgggacagcccgctcctgcacgtttaccggtacgggcggtcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataacggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAg                                                                                                                                                                         <  2:345557‑M2/113‑1 (MQ=255)                                                                                                                                   agcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGATCGGGTTTACCCGCTAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGt                                                                                                                                                >  1:285155‑M2/164‑301 (MQ=255)                                                                                                                                     cccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCCCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGGATTACCCAATAGCCGTTg                                                                                                                                              >  1:416554‑M2/162‑301 (MQ=255)                                                                                                                                        gctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAg                                                                                                                                           >  4:425985‑M2/159‑301 (MQ=255)                                                                                                                                           cctgcacgttatcctgtaggtcgcggctcgcttgcaatgcccccaaactccccctgccatacccattcccagcacctggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTcc                                                                                                                                        <  2:425876‑M2/146‑1 (MQ=255)                                                                                                                                              gcacgtttccctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATc                                                                                                                                     <  3:416556‑M2/149‑1 (MQ=255)                                                                                                                                              cgccgtctacctgtcggggcggtcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATc                                                                                                                                     <  2:191471‑M2/149‑1 (MQ=255)                                                                                                                                               cacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGTTAACCCCGCCTACAAGGCAACGCGGTACCTCGTCGGACCGGGTTTCCCCCCGCACAGGCCGGCCCAGGCTGAACACCGCTCCCAGCTACACTgtcttatccattagcccttcccgtccctct                                                                                                                                    >  2:69960‑M2/152‑287 (MQ=255)                                                                                                                                                               cttccctccacgcccctcttccgatctctccccctcccctaccccttcccagcacgtggcctttgcctatactggtgccagcctgaacccggcaacgactgccgtgtccacgccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAg                                                                                                                    <  1:364882‑M2/166‑1 (MQ=255)                                                                                                                                                                      ttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTACCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGaccacc                                                                                                             <  4:764084‑M2/173‑1 (MQ=255)                                                                                                                                                                        gcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGTGCGGGGTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTATTATCGAATAGCCGTTGCAATCCATCGCCCCTTTCACGAGCAGAaccccccc                                                                                                           >  2:409763‑M2/127‑300 (MQ=255)                                                                                                                                                                            ccctacccgacgctcttccgatctcatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCAt                                                                                                       <  1:59640‑M2/179‑1 (MQ=255)                                                                                                                                                                                cgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTc                                                                                                   <  3:89142‑M2/183‑1 (MQ=255)                                                                                                                                                                                 gcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCg                                                                                                  <  4:69979‑M2/184‑1 (MQ=255)                                                                                                                                                                                          atccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTtgcagtccatcgccccttacaagagcagagatcggaagagcacacgtctgaactc                                                                                         >  3:364883‑M2/109‑274 (MQ=255)                                                                                                                                                                                               cctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGACGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAACCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAAtaa                                                                                    >  1:127017‑M2/104‑300 (MQ=255)                                                                                                                                                                                                 tgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATAccc                                                                                  >  3:503117‑M2/102‑301 (MQ=255)                                                                                                                                                                                                  gccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATAcccc                                                                                 >  3:376132‑M2/101‑301 (MQ=255)                                                                                                                                                                                                    catacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCttacaagagcaggaccacccgccatagatcggaagagcacacgtctgaa                                                                               >  3:59642‑M2/99‑277 (MQ=255)                                                                                                                                                                                                         ccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCCAATACCCCCAGGccc                                                                          >  4:664818‑M2/94‑301 (MQ=255)                                                                                                                                                                                                               cccgcacgtggcctttgcctataatggtgccagcctgaacccggcaacgccggccgtgtccaagcccccacccagcaccgcggccgcTCCGAGCTTGGGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATACCCCCAGGCCCTGGCCg                                                                    <  2:664596‑M2/214‑1 (MQ=255)                                                                                                                                                                                                                                  tataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATACCCCCAGGCCCTGGCCGTCAGCCAGTCCGCCgcccc                                                 >  1:372808‑M2/69‑300 (MQ=255)                                                                                                                                                                                                                                                                       tgtccaagccctcgccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATACCCCCAGGCCCTGGCCGTCAGCCAGTCCGCCGCCCTCACCCCCGAAGACTACCGCCGCCTCTACCGCCAgtcg            <  3:372808‑M2/270‑1 (MQ=255)                                                                                                                                                                                                                                                                           caagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATACCCCCAGGCCCTGGCCGTCAGCCAGTCCGCCGCCCTCACCCCCGAAGCCTACCGCCGCCTCTACCGCcagtcagtcc        >  1:200159‑M2/28‑300 (MQ=255)                                                                                                                                                                                                                                                                             agccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATACCCCCAGGCCCTGGCCGTCAGCCAGTCCGCCGCCCTCACCCCCGAACACTACCGCCGCCTCTCCCGCCAGTCGGTCGaa      >  3:197162‑M2/26‑301 (MQ=255)                                                                                                                                                                                                                                                                                 ctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATACCCCCAGGCCCTGGCCGTCAGCCAGTCCGCCGCCCTCACCCCCGAAGACTACCGCCGCCTCTACCGCCAGTCGGTCGAAGAcc  <  4:409872‑M2/280‑1 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                        TCGAAAAGCTGGCCCCGCACAAGGTGATCCCGGGTAACCGCCCGAGCAACACGTTGGTGGTCGAGCGCATCAGCCCGCGCCGGCTGGGTGCGCTGGTGGCCATGTACGAGCACAAGGTGTTCGTACAGAGCGTTATCTGGGGTATCAACGCCTTCGATCAGTGGGGCGTCGAGCTGGGCAAGGAGCTGGGCAAGGGCGTTTACCAGCGCTTGGTCGGTAGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATACCCCCAGGCCCTGGCCGTCAGCCAGTCCGCCGCCCTCACCCCCGAAGACTACCGCCGCCTCTACCGCCAGTCGGTCGAAGACC  >  AE015451/5344133‑5344705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑