Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451	4,025,384	T→C	E105E (GAA→GAG)	emrA ←	multidrug efflux transport system ‑ membrane fusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	4,025,384	0	T	C	100.0%	217.0 / NA	59	E105E (GAA→GAG)	emrA	multidrug efflux transport system ‑ membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/28);  total (31/28)

GGCAGCTGCAGCCATGACATCGGGGTGCGAGGACACCACGGTGTCGTCGACCAGCGCGCTGCTGGTGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCTTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAACAGCAGGCCAAGCAGCCAAGCCTTGCGCTTGCGCGACGGCTCAGGCGCGACG  >  AE015451/4025087‑4025666                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                           ggCAGCTGCAGCCATGACATCGGGGTGCGAGGACACCACGGTGTCGTCGACCAGCGCGCTGCTGGTGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTcg                                                                                                                                                                                                                                                                                         <  4:460497/301‑1 (MQ=255)                         gTGCGAGGACACCACGGTGTCGTCGACCAGCGCGCTGCTGGTGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCtt                                                                                                                                                                                                                                                                 >  1:76920/1‑300 (MQ=255)                              ccgatctcacGGTGTCGTCGACCAGCGCGCTGCTGGTGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGc                                                                                                                                                                                                                                                            <  4:770307/294‑1 (MQ=255)                                    ccacGGTGTCGTCGACCAGCGCGCTGCTGGTGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTc                                                                                                                                                                                                                                                      >  4:374386/1‑301 (MQ=255)                                     cacGGTGTCGTCGACCAGCGCGCTGCTGGTGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGcggatcgg                                                                                                                                                                                                                                                     >  2:770027/1‑294 (MQ=255)                                                     ggggcggtgctgGGGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCt                                                                                                                                                                                                                                     <  2:414520/294‑1 (MQ=255)                                                       ttccgatcgggggCTGAGCTGTTGCCGCGGGCTGTTGCCGGCAGCCTGGGCAACGCTCAGGTCTGCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTgg                                                                                                                                                                                                                                   <  1:502241/289‑1 (MQ=255)                                                             gctgGTGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCCCTTCGCTGTCGGCCGGGTCGAATTCCAGCAATACCTGGCCGGcc                                                                                                                                                                                                                             >  2:297673/1‑300 (MQ=255)                                                                ggTGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTggagatcggaa                                                                                                                                                                                                                          >  3:502244/1‑292 (MQ=255)                                                                  tGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGAc                                                                                                                                                                                                                        >  3:537743/1‑301 (MQ=255)                                                                   gCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGAcc                                                                                                                                                                                                                       >  4:210849/1‑301 (MQ=255)                                                                     gggggtgtgggcgcgggCTGTGGACGGGAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAg                                                                                                                                                                                                                     <  2:210799/290‑1 (MQ=255)                                                                        gcttggccggctggtgttgggcggcggCCTGGGCAACGCTAAGGTGGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTc                                                                                                                                                                                                                  <  2:374296/280‑1 (MQ=255)                                                                           gTTGCCGCGCGCTGTTGCCGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGcc                                                                                                                                                                                                               <  1:537741/301‑1 (MQ=255)                                                                                     gcTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCACGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAAGACCTGGCCGGCATTGAcacggcgcccgccacccgcc                                                                                                                                                                                                     >  2:107509/1‑282 (MQ=255)                                                                                      gagttcagacgtgtgctcttccgatctaGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGccc                                                                                                                                                                                                    <  4:750507/274‑1 (MQ=255)                                                                                        gTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAGGGCCACTTCGCTTGCGGCCGGGTCGAATTGCAGCCATCACTTGACGGCATGGCCCAGGTCCCCGTCATCGGcctcc                                                                                                                                                                                                  >  2:196680/1‑298 (MQ=255)                                                                                              acgtgtgctcttccgatctaGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTg                                                                                                                                                                                            <  4:120719/282‑1 (MQ=255)                                                                                                      agacgtgtgctcttccgatctgggCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCATCTGCCGGACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGt                                                                                                                                                                                    <  4:684413/280‑1 (MQ=255)                                                                                                       ttcggacgtgtgctcttccgatctgTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTg                                                                                                                                                                                   <  3:132987/277‑1 (MQ=255)                                                                                                                 aGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCAGAtcggaagagcggcgtggagggcaa                                                                                                                                                                         >  2:750228/1‑278 (MQ=255)                                                                                                                 aGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCCGCCCCgcagcttggaccggaaaggcgtccgg                                                                                                                                                                         >  2:120694/1‑276 (MQ=255)                                                                                                                  ggTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAg                                                                                                                                                                        <  4:297741/301‑1 (MQ=255)                                                                                                                           gggCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCATCTGCCGGACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGtaggtcggaaggacggcggggg                                                                                                                                                               >  2:684183/1‑280 (MQ=255)                                                                                                                               gTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCCGCTTGGCCTCGGTGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCAACGGCCCCGATGCAGGTAACGTTGCCAGCGAACATCGGGGTgtggtcc                                                                                                                                                           >  2:511579/1‑295 (MQ=255)                                                                                                                               gTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTgagatcggaagagcgtcgtggaggg                                                                                                                                                           >  1:132985/1‑277 (MQ=255)                                                                                                                                     cAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACg                                                                                                                                                     >  3:369931/1‑301 (MQ=255)                                                                                                                                         tcttctGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCCGCGACCAGCCGGGTGATTTCCACCACGTTGc                                                                                                                                                 >  2:148765/1‑301 (MQ=255)                                                                                                                                             ctGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGGTTTGCACCACGTTGCAGtt                                                                                                                                             >  1:98633/1‑301 (MQ=255)                                                                                                                                                cGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGAc                                                                                                                                          >  3:525576/1‑301 (MQ=255)                                                                                                                                                cGGCAATCGCACAGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTAGCCGTCATCGGACACGATGCTGGTAAAGGTGCCAGCGAACAGCGGGGTGATTTCAACAACGTTGcatttgag                                                                                                                                          >  1:145287/1‑294 (MQ=255)                                                                                                                                                     aTCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAgg                                                                                                                                     <  3:76922/301‑1 (MQ=255)                                                                                                                                                               tGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGt                                                                                                                           <  1:369931/301‑1 (MQ=255)                                                                                                                                                                        gcACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTg                                                                                                                  >  1:20914/1‑301 (MQ=255)                                                                                                                                                                          cccTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGcc                                                                                                                <  4:107533/300‑1 (MQ=255)                                                                                                                                                                           ccTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCa                                                                                                               <  4:148803/301‑1 (MQ=255)                                                                                                                                                                            cTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCaa                                                                                                              >  3:67515/1‑301 (MQ=255)                                                                                                                                                                                cgcCGTTTTAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTTTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTATGCATCGTCGGTGCTTTCGTGCCAACGcc                                                                                                          <  3:145289/301‑1 (MQ=255)                                                                                                                                                                                            tCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGcccagatcggaaga                                                                                              >  4:658419/1‑290 (MQ=255)                                                                                                                                                                                            tCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGAGTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGGTCGTTCGTGCCAAAGCCCGACAATGCTGt                                                                                              >  1:620391/1‑301 (MQ=255)                                                                                                                                                                                             ccTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTa                                                                                             <  3:620393/301‑1 (MQ=255)                                                                                                                                                                                               ggTTGAGCCTTGCGCAGTTAGGCTTGGCGGNTTTCCAGGTGANCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCGGCCGAACGCTGCGNGCCAGCTTGGACTCGGCGGACTGCAAGGCAACATCGCTGTCGGCCGGGTNGAATAGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTAGTGCCAACGCCCGACAATGCTGTAcc                                                                                           <  3:98636/300‑1 (MQ=255)                                                                                                                                                                                                  tGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAgg                                                                                        <  3:20914/301‑1 (MQ=255)                                                                                                                                                                                                        ttGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAgg                                                                                  <  1:525574/301‑1 (MQ=255)                                                                                                                                                                                                                     cTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGGCCCCGGc                                                                     >  2:130484/1‑301 (MQ=255)                                                                                                                                                                                                                                tCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATcagcc                                                          >  1:277394/1‑300 (MQ=255)                                                                                                                                                                                                                                 ccAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGTGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATcagcag                                                         <  4:511729/301‑1 (MQ=255)                                                                                                                                                                                                                                   aGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGCCCCCGGCAAGGATcaaccagc                                                       >  2:560542/1‑295 (MQ=255)                                                                                                                                                                                                                                          ccTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAAcagcag                                                <  1:67513/301‑1 (MQ=255)                                                                                                                                                                                                                                                 ggagttcagacgtgtgctcttccgatctaCCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAACAGCAGGCCAagc                                         <  4:188661/273‑1 (MQ=255)                                                                                                                                                                                                                                                  gAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAACAGCAGGCCAagca                                        >  4:741436/1‑301 (MQ=255)                                                                                                                                                                                                                                                             gCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAACAGCAGGCCAAGCAGCCAAGCcttg                             <  4:130513/301‑1 (MQ=255)                                                                                                                                                                                                                                                               tGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAACAGCAGGCCAAGCAGCCAAGCcttgcg                           >  4:610657/1‑301 (MQ=255)                                                                                                                                                                                                                                                               ggTAAAGCCCGCGAACCGGCCGAACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAACAGCAGGCCAAGCAGCCAAGCcttgcg                           <  2:610460/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        cgcgCACCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAGGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAACAGCAGGCCAAGCAGCCAAGCCTTGCGCTTGCGCGa                  <  4:196726/301‑1 (MQ=255)                                                                                                                                                                                                                                                                             aCCTGCCGCACGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAACAGCCGGCCAagcagactggaagagcgtcgggtggggaaag             >  2:188615/1‑275 (MQ=255)                                                                                                                                                                                                                                                                                     aaCGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAACAGCAGGCCAAGCAGCCAAGCCTTGCGCTTGCGCGACGGCTCAGGcgcg     <  3:277393/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                       cGCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGCTCAGCAGCAACAGCAGGCCAAGCAGCCAAGCCTTGCGCTTGCGCGCCGGCCCAGgccggac   >  4:669861/1‑296 (MQ=255)                                                                                                                                                                                                                                                                                        gCTGCGTGCCAGCTTGGCCTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAACAGCAGGCCAAGCAGCCAAGCCTTGCGCTTGCGCGACGGCTCAGGCGCGACg  >  3:380478/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                           GGCAGCTGCAGCCATGACATCGGGGTGCGAGGACACCACGGTGTCGTCGACCAGCGCGCTGCTGGTGCTGAGCTGTTGCCGCGCGCTGTTGACGGCAGCCTGGGCAACGCTCAGGTCGTCACGGGCGTGGGACAGTTCTTCTGCGGCAATCGCACCGCTGTCAGCCAGCACCTTGCGCCGGTTGAAGTCCTGTTGCGCCTTGCGCAGTTCGGCTTGGCGCGTTTCCAGTTGAGCCTTCAGCGAGTCGACGTTGCTGTAAAGCCCGCGCACCTGCCGCACGCTGCGTGCCAGCTTGGCTTCGGCGGACTGCAAGGCCACTTCGCTGTCGGCCGGGTCGAATTGCAGCAATACCTGGCCGGCATGGACCAGGTCGCCGTCATCGGCCCCGATGCTGGTAACGGTGCCAGCGACCAGCGGGGTGATTTCCACCACGTTGCCGTTGACGTAGGCATCGTCGGTGCTTTCGTGCCAACGCCCGACAATGCTGTACCAGGCCCAGGTGCCGACCCCGGCAAGGATCAGCAGCAACAGCAGGCCAAGCAGCCAAGCCTTGCGCTTGCGCGACGGCTCAGGCGCGACG  >  AE015451/4025087‑4025666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑