| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AE015451 | 353,067 | A→G | intergenic (+34/+84) | hisF → / ← cbcV | Imidazole glycerol phosphate synthase subunit HisF/choline / betaine / carnitine ABC transporter ‑ ATP binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 353,067 | 0 | A | G | 98.0% | 166.9 / NA | 50 | intergenic (+34/+84) | hisF/cbcV | Imidazole glycerol phosphate synthase subunit HisF/choline / betaine / carnitine ABC transporter ‑ ATP binding subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (23/26); minor base C (1/0); total (24/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAGATCCTGCTGACCAGCATGGACCAGGACGGCATGAAGAACGGCTTCGACCTGGGCGTTACCCGGGCTATCAGTGATGCGCTGGGGATTCCGGTGATTGCCTCGGGGGGGGTGGGTAACCTGCAGCACCTGGCAGACGGCATTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCAGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCA‑CCACCCTGTCGTTATCCTGCAGCA‑CCAGCTTGTTGCCGGTTTGATAACGAATCTGCAGTGCCTCACGCATGCCGATGTCGGCGTGCACCACGGTCGGCCTGCGCTCCAGCAGCTCCACATCTTGCCCCGGTGCCCATTTCTGCATGTCCAG > AE015451/352779‑353360 | aagaTCCTGCTGACCAGCATGGACCAGGACGGCATGAAGAACGGCTTCGACCTGGGCGTTACCCGGGCTATCAGTGATGCGCTGGGGATTCCGGGGATTGCCTCGGGGGGGGTGGGTAACCTGCAGCACCTGGCAGACGGCATTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCt < 3:523741/300‑1 (MQ=255) tgctgACCAGCATGGACCAGGACGGCATGAAGAACAGCTTCGACCTGGGCGTTACCCGGGCTATCAGTGATGCGCTGGGGATTCCGGTGATTGCCTCGGGGGGGGTGGGTAACCTGCAGCACCTGGCAGACGGCATTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGATTTGCAGCCCTTCGCGGGCACGCCTGCCCCcaccg > 1:147451/1‑299 (MQ=255) ctgACCAGCATGGACCAGGACGGCATGAAGAACGGCTTCGACCTGGGCGTTACCCGGGCTATCAGTGATGCGCTGGGGATTCCGGTGATTGCCTCGGGGGGGGTGGGTAACCTGCAGCACCTGGCAGACGGCATTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGGTGAGCCATTGAACGCGGCTGCTTTGCAGCCCTTCGCGGGCACGGCCGCGCCCACAggg > 1:570554/1‑300 (MQ=255) tgACCAGCATGGACCAGGACGGCATGAAGAACGGCTTCGACCTGGGCGTTACCCGGGCTATCAGTGATGCGCTGGGGATTCCGGTGATTGCCTCGGGGGGGGTGGGTAACCTGCAGCACCTGGCAGACGGCATTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGACTACATGGCTTCGCGAGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGAGGGCACGCACGCTCACACAGGtg > 1:338088/1‑300 (MQ=255) cccAGCATGGACCAGGACGGCATGAAGATCGGCTTCGACCTGTGCGTTACCCGGGCTATCAGTGATGCGCTGGGGATTCCGGTGATTGCCTCGGGGGGGGTGGGTAACCTGCAGCACCTGGCAGACGGCATTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCTCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTg < 3:338089/300‑1 (MQ=255) ggCATGAAGAACGGCTTCGACCTGGGCGTTACCCGGGCTATCAGTGATGCGCTGGGGATTCCGGTGATTGCCTCGGGGGGGGTGGGTAACCTGCAGCACCTGGCAGACGGCATTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAg < 3:257836/301‑1 (MQ=255) cagacgtgtgctcttccgatcTGGGCGTTACCCGGGCTATCAGTGATGCGCTGGGGATTCCGGTGATTGCCTCGGGGGGGGTGGGTAACCTGCAGCACCTGGCAGACGGCATTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGc < 3:98802/281‑1 (MQ=255) tGAAGAACGGCTTCGACCTGGGCGTTACCCGGGCTATCAGTGATGCGCTGGGGATTCCGGTGATTGCCTCGGGGGGGGTGGGTAACCTGCAGCACCTGGCAGACGGCATTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTTCAGCCCTTCGCCGGCACCCCCCCCCCCACCTGCTTGCTgagcactcctgtggagcggg > 2:309286/1‑284 (MQ=255) ggaagaaCGGCTTGGACCTGGGCGTGACCCGGGCTATCAGTGATGCGCTGGGGATTCCGGTGATTGCCTCGGGGGGGGTGGGTAACCTGCAGCACCTGGCAGACGGCATTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCggg < 1:161204/300‑1 (MQ=255) ggcttagcccTGGGCGTTCCCCGGGCTATAAGTGATGCGCTGGGGATTCCGGTGATTGCCTCGGGGGGGGTGGGTAACCTGCAGCACCTGGCAGACGGCATTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCcg < 3:570556/293‑1 (MQ=255) ggCTTCGACCTGGGCGTTACCCGGGCTATCAGTGATGCGCTGGGGATTCCGGTGATTGCCTCGGGGGGGGTGGGTAACCTGCAGCACCTGGCAGACGGCATTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCcg < 1:363507/301‑1 (MQ=255) cTTCGACCTGGGCTTTACCCGGGCTATCAGTGATGCGCTGGGGATTCCGTTGATTGCCTCGTGGGGGGTGGGTAACCTGCAGCACCTGGCAGACGGCATTCTGGAAGGGAATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCcgcg < 3:147453/301‑1 (MQ=255) cccTGGGCGTTACCCGGGCTATCAGTGATGCGCTGGGGATTCCGGTGATTGCCTCGGGGGGGGTGGGTAACCTGCAGCACCTGGCAGACGGCATTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACGTGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAggg < 4:629781/300‑1 (MQ=255) ccTGGGCGTTACCCGGGCTATCAGTGATGCGCTGGGGATTCCGGTGATTGCCTCGGGGGGGGTGGGTAACCTGCAGCACCTGGCAGACGGCATTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGCTCTCTCTGGGCGCGGGCGCGCCcgctgaggcg > 2:629578/1‑294 (MQ=255) gggCGTTACCCGGGCTATCAGTGATGCGCTGGGGATTCCGGTGATTGCCTCGGGGGGGGTGGGTAACCTGCAGCACCTGGCAGACGGCATTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACTGGTTTTCTGCGTTCTCtgttggtgcagctcggaagaccggtgtgttt > 1:98799/1‑273 (MQ=255) ttccgatctggctcgcggTGATGCGCTGAGCATCCGGGTGGTGGCCTCGGGGGGGGTGGGTAACCTGCAGCACCTGGCAGACGGCATTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGAGCGTGCCCGCGAAGGGCCGCGaa < 3:112524/284‑1 (MQ=255) gTTACCCGGGCTATCAGTGATGCGCTGGGGATTCCGGTGATTGCCTC‑GGGGGGGTGGGTAACCTGCAGCACCTGGCAGACGGCATTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGTTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACCGGTTTGCTGAGCTCTCTGTGGGAGCCGGCGTGCCCGCGAAGGGCCGCGCAGc > 4:256850/1‑301 (MQ=255) tACCCGGGCTATCAGTGATGCGCTGGGGATTCCGGTGATTGCCTCGGGGGGGGTGGGTAACCTGCAGCACCTGGCAGACGGCATTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCg > 1:231559/1‑301 (MQ=255) ccgctgtccctacacgactctctgccgatcccGGTGATTGCCTCGGGGGGGGTGGGTAACCTGCAGCACCTGGCAGACGTCATTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACGTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCgg < 1:110532/271‑1 (MQ=255) ggCTATCAGTGATGCGCTGGGGATTCCGGTGATTGCCTCGGGGGGGGTGGGTAACCTGCAGCACCTGGCAGACGGCATTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGaaagatcggag > 1:112521/1‑292 (MQ=255) cgatctggTGATTGCCTCGGGGGGGGTGGGTAACCTGCAGCACCTGGCAGACGGCATTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCtt < 4:518022/295‑1 (MQ=255) ggTGATTGCCTCGGGGGGGGTGGGTAACCTGCAGCACCTGGCAGACGGCATTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCCCCGTGGGAGCGGGCGTGCCCGTGAAGGGCCGCGCAGCGGCCCCAATTGCTTCCACCgctgttcctattgtc > 2:517872/1‑287 (MQ=255) ggATTGCCTCGGGGGGGGTGGGTAACCTGCAGCACCTGGCAGACGGCATTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGagca < 3:231557/300‑1 (MQ=255) ccTCGGGGGGGGTGGGTAACCTGCAGCACCTGGCAGACGGCATTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGcgcgg > 1:471878/1‑300 (MQ=255) atcttGGCAGACGGCATTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTAT‑CCCGAGTATcc < 4:106148/297‑1 (MQ=255) tGGCAGACGGCATTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGTCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGCAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCCGCGCGTGGTAAAGCCCGGGATcccggcgatccagca > 2:106124/1‑289 (MQ=255) ggCAGACGGCATTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTGGGTATCCCCGAGTAtatccac > 4:103431/1‑295 (MQ=255) ggCATTATGGAAGGGCAGGCCAGCGCGGTGCTGGCTGCCAGCAGCTTCCACTTTGGCG‑AGTACACAGGGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCA‑CCACCCTg < 2:125481/301‑1 (MQ=255) cgcaccctctccgatccaaGCGCGGTGCGGGCGGCCACCATCTT‑CACCTTGGCGTAGTACACCGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCA‑CCACCCTGTCg < 2:653130/283‑1 (MQ=255) cATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGCTCTTGCCGAGCAGCTCGTGGTACAGCTCGGTATCCCCGAGTATCCCCA‑CCACCCTGTCGTTATCCTgcagc > 4:246376/1‑301 (MQ=255) cAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCA‑CCACCCTGTCgagatcggaagagcacac > 4:653341/1‑284 (MQ=255) ggggggggcgggcggcCAGCAAGTTCAACTGTGGCG‑GGTACACAGTGCCGGAGGCCAAGGCCAACATGGCTTCGCGCGGGATCGTCGTTGGCTGATCCATTGACCGGGGCTGTTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCA‑CCACCCTGTCGTTATCCTGCAGCA‑CCAGCt < 2:256792/291‑1 (MQ=255) ggcgGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCA‑CCACCCTGTCGTTATCCTGCAGCA‑CCAGCtt < 4:309359/300‑1 (MQ=255) cTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCCCGCCAGCTCCCCCAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCACCGCGGGGTAAAGCCCGGTATCCCCGAGTATCCCCA‑CCACCCGGTCGTTagatcggaagagaacctgtctgaac > 4:83840/1‑277 (MQ=255) ggcCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCA‑CCACCCTGTCGTTATCCTGCAGCA‑CCAGCTTGTTGCCGGTTTg < 3:471880/301‑1 (MQ=255) aGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCAAATTGCTTAAACCGTGGTTTTTGCCGAGCAGCGCGTGGTAAAGCCCGGTTTCCCAGCGTA‑CCCCACCCACCCTGTCGTTATCTTGCA‑CACCCAGCTTATTGCCGGTTTGATaa > 1:80754/1‑301 (MQ=255) tCCACTTTGGCG‑AGTACACATGGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTTCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCA‑CCACCCTGTCGTTATCCTGCAGCA‑CCAGCTTGTTGCCGGTTTGATAACGAATCt < 3:80756/301‑1 (MQ=255) tttcccctccacgccgcctttcctatctggCCAAGGCCTACAGGGCTTCGCGCGGGTTCGTCGTTCGCTGATCCATTGCCCGGGGCGGCTTTGCAGCCTTCCGCGGGCACGCCCGTTCCAACAGGTTTGCTGGGATCTCTGT‑GGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCA‑CCACCCTGTCGTTATCCTGCAGCA‑CCAGCTTGTTGCCGGTTTGATAACGAATCTg < 1:447841/273‑1 (MQ=255) cgtaatgtgtagagaaccgagggCCGGAGGCAAAGGCCTACATGGCGTCGCGATGGATCGTGGTTCGCTGATCCATTGACCGGAGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTTCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCA‑CCACCCTGTCGTTATCCTGCAGCA‑CCAGCTTGTTGCCGGTTTGATAACGAATCt < 2:103407/279‑1 (MQ=255) aGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCA‑CCACCCTGTCGTTATCCTGCAGCA‑CCAGCTTGTTGCCGGTTTGATAACGAATCTGCAGTGCCTCACGCATGCCGATGTCgg > 4:531854/1‑301 (MQ=255) ggCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGT‑GGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCA‑CCACCCTGTCGTTATCCTGCAGCA‑CCAGCCTGTTGCCGGTTTGATAACGGATCTgagatcggaagagcaaacgtctgaactcc > 3:447843/1‑273 (MQ=255) atctaaGGCCTACCTGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCA‑CCACCCTGTCGTTATCCTGCAGCA‑CCAGCTTGTTGCCGGTTTGATAACGAATCTGCAGTGCCTCACGCATGCCGATGTCGGc < 4:161920/297‑1 (MQ=255) aaGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCA‑CCACCCTGTCGTTCTCCTGCAGCA‑CCAGCTTGTTGCCGGTTTGATCACTAATCTGCAGTCCCTCCCGCATGCCGATGTTGGccgat > 2:161882/1‑297 (MQ=255) aGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCA‑CCACCCTGTCGTTATCCTGCAGCA‑CCAGCTTGTTGCCGGTTTGATAACGAATCTGCAGTGCCTCACGCATGCCGATGTCGGCGTGca > 4:736154/1‑301 (MQ=255) gctcttccaatctcgtTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCA‑CCACCCTGTCGTTATCCTGCAGCA‑CCAGCTTGTTGCCGGTTTGATAACGAATCTGCAGTGCCTCACGCATGCCGATGTCGGCGTGCACCACGGTCGGCCTgc < 4:589189/289‑1 (MQ=255) gggATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCA‑CCACCCTGTCGTTATCCTGCCGCA‑CCAGCTTGTTGCCGGTTTGATAACGAATCTGCAGTGCCTCACCCATGCCGATGTCGGGGTGCACCCCGGGCGGCCtcggcccc > 2:335905/1‑294 (MQ=255) ggaccgtggtgcgcggttCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCA‑CCACCCTGTCGTTATCCTGCAGCA‑CCAGCTTGTTGCCGGTTTGATAACGAATCTGCAGTGCCTCACGCATGCCGATGTCGGCGTGCACCACGGTCGGCCTGCGCTCca < 2:735894/284‑1 (MQ=255) cgtTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCA‑CCACCCTGTCGTTATCCTGCAGCA‑CCAGCTTGTTGCCGGTTTGATACCGAATCTGCAGTGCCTCACGCATGCCGATGTCGGCTTGccccccgtctcgccctccactcggtcgcac > 2:589004/1‑272 (MQ=255) ccagagcacgggcatgattcgCAGCCCGTCGCGGGCACGCCCGTGCCCACAGGTTGGCTCAGATCTCGGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCA‑CCACCCTGTCGTTATCCTGCAGCA‑CCAGCTTGTTGCCGGTTTGATAACGAATCTGCAGTGCCTCACGCATGCCGATGTCGGCGTGCACCACGGTCGGCCTGCGCTCCAGCAGCTCCACATCTTGcc < 2:531704/281‑1 (MQ=255) ccTTCGCGGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCA‑CCACCCTGTCGTTATCCTGCAGCA‑CCAGCTTGTTGCCGGTTTGATAACGAATCTGCAGTGCCTCACGCATGCCGATGTCGGCGTGCACCACGGTCGGCCTGCGCTCCAGCAGCTCCACATCTTGCCCCGGTGCCCATTTCTGCATGTCCac > 1:13365/1‑300 (MQ=255) | GAGATCCTGCTGACCAGCATGGACCAGGACGGCATGAAGAACGGCTTCGACCTGGGCGTTACCCGGGCTATCAGTGATGCGCTGGGGATTCCGGTGATTGCCTCGGGGGGGGTGGGTAACCTGCAGCACCTGGCAGACGGCATTCTGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCG‑AGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGCTGCTTTGCAGCCCTTCGCAGGCACGCCCGCTCCCACAGGTTTGCTGAGATCTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATCCCCA‑CCACCCTGTCGTTATCCTGCAGCA‑CCAGCTTGTTGCCGGTTTGATAACGAATCTGCAGTGCCTCACGCATGCCGATGTCGGCGTGCACCACGGTCGGCCTGCGCTCCAGCAGCTCCACATCTTGCCCCGGTGCCCATTTCTGCATGTCCAG > AE015451/352779‑353360 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |