Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451	4,889,556	G→A	intergenic (+2/‑151)	hyi → / → glxR	hydroxypyruvate isomerase/tartronate semialdehyde reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	4,889,556	0	G	A	100.0%	131.2 / NA	37	intergenic (+2/‑151)	hyi/glxR	hydroxypyruvate isomerase/tartronate semialdehyde reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/21);  total (16/21)

GCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTCCACCCACCACGACGCCGCGCCAG  >  AE015451/4889267‑4889818                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                       gCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCggg                                                                                                                                                                                                                                                             <  4:563433/301‑1 (MQ=255)   cGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGAc                                                                                                                                                                                                                                                           <  3:70326/301‑1 (MQ=255)   cGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGAc                                                                                                                                                                                                                                                           >  4:274042/1‑301 (MQ=255)                   aaCCTGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCt                                                                                                                                                                                                                                           <  4:178945/301‑1 (MQ=255)                    aCCTGTTCCTGCAATACGAAATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTc                                                                                                                                                                                                                                          <  2:782254/301‑1 (MQ=255)                     gatcttCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCg                                                                                                                                                                                                                                         <  2:150209/297‑1 (MQ=255)                         ttCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTTGcccc                                                                                                                                                                                                                                     >  2:452916/1‑300 (MQ=255)                          tCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCgagatc                                                                                                                                                                                                                                    >  4:150247/1‑296 (MQ=255)                            cTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGACTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGa                                                                                                                                                                                                                                  <  2:481910/301‑1 (MQ=255)                                  aaCGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCg                                                                                                                                                                                                                            <  1:364396/300‑1 (MQ=255)                                      aCATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCaa                                                                                                                                                                                                                        >  4:500334/1‑301 (MQ=255)                                        aTCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAg                                                                                                                                                                                                                      >  4:284222/1‑301 (MQ=255)                                                      gATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGTAGCGGGcccgcccggtcaccccggccaagcccggtcccaccac                                                                                                                                                                                                        >  2:630974/1‑272 (MQ=255)                                                      gATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCAc                                                                                                                                                                                                        >  4:478879/1‑301 (MQ=255)                                                             gAAGGTGACCTGGCTCGAACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTg                                                                                                                                                                                                 <  2:478745/301‑1 (MQ=255)                                                                        ggCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTcgcg                                                                                                                                                                                      <  1:138872/301‑1 (MQ=255)                                                                          cTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCggg                                                                                                                                                                                    >  3:105777/1‑301 (MQ=255)                                                                               aCCATGGAAGCCAACCAGAAGCTGATCAACCCCCTCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGAGCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGc                                                                                                                                                                               <  2:273976/301‑1 (MQ=255)                                                                                cacgcgacgctcttccgatcttGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGcc                                                                                                                                                                              <  2:545192/280‑1 (MQ=255)                                                                                          cAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTTTCTTCTTCGCGGGCGAGCCCGCGcccccc                                                                                                                                                                    >  1:417428/1‑298 (MQ=255)                                                                                              cTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAgggg                                                                                                                                                                <  3:620854/301‑1 (MQ=255)                                                                                                 aaGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTTGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCAGCTCCCACAGGGGGcc                                                                                                                                                             <  4:453045/301‑1 (MQ=255)                                                                                                     tGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGccagatcggaagagcacacgtct                                                                                                                                                         >  4:545349/1‑280 (MQ=255)                                                                                                            ccACATCCGCCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAgcttgctt                                                                                                                                                  <  1:106980/301‑1 (MQ=255)                                                                                                               cacccacccggacgacaacaccagcagCAATGAACCCGGCACCGACGATATCAACTACCTCGTCCTGTCCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGGACAAGCCGCTGACCACCACCGAAGCGGGCCGGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTgag                                                                                                                                               <  2:284154/275‑1 (MQ=255)                                                                                                                                             agAACCAGGAACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCa                                                                                                                 <  3:417430/300‑1 (MQ=255)                                                                                                                                                          ggCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCgg                                                                                                    >  4:267635/1‑301 (MQ=255)                                                                                                                                                               aaTCAACTACCCCTTCCTGTTCGAAAACCGGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCa                                                                                               <  2:267569/300‑1 (MQ=255)                                                                                                                                                                         cGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCgg                                                                                     <  4:631177/301‑1 (MQ=255)                                                                                                                                                                                           cacacgacgctcttccgatctggCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCAt                                                                   <  1:266645/280‑1 (MQ=255)                                                                                                                                                                                                      ttcagacgtgtgctcttccgatctggAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAAcc                                                        <  3:545277/277‑1 (MQ=255)                                                                                                                                                                                                                ggCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCAtagatcggaagagcacacgtct                                              >  3:266644/1‑280 (MQ=255)                                                                                                                                                                                                                              ggAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAAccagatcggacgagcgtcgtgtaggg                                >  1:545275/1‑277 (MQ=255)                                                                                                                                                                                                                                            tGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTccacccac                  >  3:416535/1‑301 (MQ=255)                                                                                                                                                                                                                                             gACCACAACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTCcacccacc                 <  1:105774/301‑1 (MQ=255)                                                                                                                                                                                                                                                      cGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTACAATCGGTTTCATCGGCACCGGCATCATTGGCAAGCCCATGGCTCAGAACCTGCAAACAGCAGGTCCAAGCCTGTTCATTTCCACCCACCACGAcgccc        >  1:501375/1‑300 (MQ=255)                                                                                                                                                                                                                                                            gggCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTCCACCCACCACGACGCCGCGCCAg  >  4:774207/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                       GCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTCCACCCACCACGACGCCGCGCCAG  >  AE015451/4889267‑4889818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑