Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451 5,674,753 G→C intergenic (+115/+25) rhlE‑II → / ← yceI ATP‑dependent RNA helicase/polyisoprene‑binding protein, acidic stress response factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154515,674,7530GC98.0% 181.1 / ‑3.5 51intergenic (+115/+25)rhlE‑II/yceIATP‑dependent RNA helicase/polyisoprene‑binding protein, acidic stress response factor
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base C (22/28);  total (22/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

ACGATGCTGCCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑GCGCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCATGGTCAGGTCGCCAGTCACGTCAGCCGTCTTGTCGCCAGTAGACTTGACGCTGGTGGAGACGAATTTGGCGT  >  AE015451/5674466‑5675043
                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                    
ccgaggctgcCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTc                                                                                                                                                                                                                                                                                        <  1:43749/296‑1 (MQ=255)
              aaaGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAAtt                                                                                                                                                                                                                                                                          <  3:54828/300‑1 (MQ=255)
               aaGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAAttt                                                                                                                                                                                                                                                                         >  1:54826/1‑300 (MQ=255)
                agagCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAACGCGGCCCC‑‑CTCGGTTTTCAGATGGCAGAAATTAc                                                                                                                                                                                                                                                                        >  2:100532/1‑301 (MQ=255)
                agagCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTAc                                                                                                                                                                                                                                                                        <  3:608644/301‑1 (MQ=255)
                         ccgatctaaCCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTc                                                                                                                                                                                                                                                               <  3:85059/294‑1 (MQ=255)
                              gcAACCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACAcc                                                                                                                                                                                                                                                          <  1:204329/301‑1 (MQ=255)
                                aaCCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTcagatcgg                                                                                                                                                                                                                                                        >  1:85056/1‑295 (MQ=255)
                                aaCCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCtt                                                                                                                                                                                                                                                        <  4:100553/301‑1 (MQ=255)
                                      cgTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGacac                                                                                                                                                                                                                                                  <  1:1143/301‑1 (MQ=255)
                                      cgTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGacac                                                                                                                                                                                                                                                  <  4:589288/301‑1 (MQ=255)
                                      cgTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGacac                                                                                                                                                                                                                                                  >  3:1143/1‑301 (MQ=255)
                                           gCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGata                                                                                                                                                                                                                                             >  4:701196/1‑301 (MQ=255)
                                                         cagggagcATTCGCAAGGGGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGt                                                                                                                                                                                                                               <  2:644173/294‑1 (MQ=255)
                                                          aGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTc                                                                                                                                                                                                                              >  1:632016/1‑301 (MQ=255)
                                                               tCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGa                                                                                                                                                                                                                         <  3:632018/301‑1 (MQ=255)
                                                                           ggTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACTTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCgg                                                                                                                                                                                                             <  1:238030/300‑1 (MQ=255)
                                                                            gTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATATGGCCGCAACGCGGCCCC‑‑CTCGGCTTTCCGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGCAGTAGCACCCgtg                                                                                                                                                                                                            >  4:722587/1‑299 (MQ=255)
                                                                              ccAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGcc                                                                                                                                                                                                          >  3:110208/1‑301 (MQ=255)
                                                                                aagcacgACCGTTTCCCGACGCCGGAGCAACACGATGACCTGCCAAGCCGCCCACGCGGTGGGCGACCAACGCAGCTGACCCGCAATTGCTCATAGAAGCACCAAGAG‑GGCTGCTTCGCATCCCAATCACGAAACAAGGCCGCTCCTGCAGTGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTAGTGATAGGGCCGCAAAGC‑G‑GCCGACTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCtt                                                                                                                                                                                                        <  2:722342/295‑1 (MQ=255)
                                                                                 gacgtgtgctcttccgatctccTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCttt                                                                                                                                                                                                       <  4:153719/281‑1 (MQ=255)
                                                                                  ggtGGATCGTTTCCCGCGGCCGTAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTg                                                                                                                                                                                                      <  2:717515/299‑1 (MQ=255)
                                                                                   cTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGa                                                                                                                                                                                                     >  4:566343/1‑301 (MQ=255)
                                                                                                     ccTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCCCCCGGGCCtttagagcggaagagcgtcgtgt                                                                                                                                                                                   >  2:153681/1‑281 (MQ=255)
                                                                                                        gAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggttca                                                                                                                                                                                <  1:110205/301‑1 (MQ=255)
                                                                                                            cacggaacgcccggcgaggctgccCACGCAGTGCGCGCCCGGCGCTGCTGACCCGCAATGGCTGTAAGAATTACC‑AGGGTGGCTCCTTCGCAGCCCTATCACGACGCAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgt                                                                                                                                                                            <  2:700951/280‑1 (MQ=255)
                                                                                                               tGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggg                                                                                                                                                                         >  1:18137/1‑300 (MQ=255)
                                                                                                                                         ggTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGc                                                                                                                                               >  4:293998/1‑301 (MQ=255)
                                                                                                                                               ggcCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTCCCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCAcccc                                                                                                                                         <  4:623111/300‑1 (MQ=255)
                                                                                                                                                      ggctgctGACCCGCAAGGGCTGGTAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTc                                                                                                                                  <  2:566173/300‑1 (MQ=255)
                                                                                                                                                         cgctgACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCCCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAAATACTTCTGCTTCACCCCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCtt                                                                                                                               <  4:204643/300‑1 (MQ=255)
                                                                                                                                                                          tGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAgg                                                                                                              <  3:26077/301‑1 (MQ=255)
                                                                                                                                                                             aaGAAGTACCGAGGC‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAg                                                                                                           <  1:564469/300‑1 (MQ=255)
                                                                                                                                                                                            tcagacgtgtgctcttccgatctgACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCAcc                                                                                             <  4:728601/278‑1 (MQ=255)
                                                                                                                                                                                                  ctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTg                                                                                       <  3:18137/301‑1 (MQ=255)
                                                                                                                                                                                                   ttCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTgg                                                                                      >  1:74732/1‑301 (MQ=255)
                                                                                                                                                                                                     tccccgccccccttccgatcttgcctcTCCTGAAGAGAATTTCTTGATCTCTACAGGGGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTg                                                                                    <  2:370893/275‑1 (MQ=255)
                                                                                                                                                                                                                   gACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCAccagatcggaagagcgtcgggtagg                                                                      >  2:728341/1‑278 (MQ=255)
                                                                                                                                                                                                                   gACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCATGGt                                                                      >  3:597603/1‑301 (MQ=255)
                                                                                                                                                                                                                          ggCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGagatcggaagaggacacgtcc                                                               >  4:370983/1‑281 (MQ=255)
                                                                                                                                                                                                                                 ccTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCATGGTCAGGTCGCCAGTcc                                                        >  4:528657/1‑300 (MQ=255)
                                                                                                                                                                                                                                  agttcagacgtgtgctcttccgatctggAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCATGGTCAGGTCGCCAGTCAc                                                       <  3:29868/275‑1 (MQ=255)
                                                                                                                                                                                                                                      cttgaATTTCTTGATCTCTACAGGAGCGGCCTTGGGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAGCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCATGGTCAGGTCGCCAGTCACGTCa                                                   <  2:126478/298‑1 (MQ=255)
                                                                                                                                                                                                                                        agaATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCATGGTCAGGTCGCCAGTCACGTCaag                                                 >  4:126507/1‑299 (MQ=255)
                                                                                                                                                                                                                                          aaTTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCATGGTCAGGTCGCCAGTCACGTCAGCCg                                               <  3:74734/301‑1 (MQ=255)
                                                                                                                                                                                                                                                 gacgctcttccgatctggCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCATGGTCAGGTCGCCAGTCACGTCAGCCGTCTTGTc                                        <  2:179786/285‑1 (MQ=255)
                                                                                                                                                                                                                                                          cAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCATGGTCAGGTCGCCAGTCACGTCAGCCGTCTTGTCGCCAGTAgg                               >  4:550916/1‑300 (MQ=255)
                                                                                                                                                                                                                                                            ggAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCATGGTCAGGTCGCCAGTCAcagatcggaagagcgtcgtgtagggaa                             >  1:29868/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                 ggCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCATGGTCAGGTCGCCAGTCACGTCAGCCGTCTTGTcagatcggaagagcaca                        >  4:179828/1‑285 (MQ=255)
                                                                                                                                                                                                                                                                                     ccGCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCATGGTCAGGTCGCCAGTCACGTCAGCCGTCTTGTCGCCAGTAGACTTGACGCTGGTGGAGACGAATTTGGc    <  1:597601/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                       gCAAAGCGGCCCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAGGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCATGGTCAGGTCGCCAGTCACGTCAGCCGTCTTGTCGCCAGTAGACTTGACGCTGGTGGAGACGAATTTGGCGt  >  4:620234/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                    
ACGATGCTGCCCGGCAAGAGCCGGCCGTGCGCAACCCGCGTCAGCCGGAAAAACAGCCAGTGATCATTCGCAAGGAGTCCAAGCTGGACCGTTTCCCGACGCCTGAGCAACTGGATGACCTGCCAAGCCGCCCACGCGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGG‑GGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑GCGCC‑‑CTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCCAGCGCGCTCGCCACCCCATGGGTCCTTGCCTTCACCGTTGAAGGTAGCCTTGAACGTCACCGGCTTGGTGACACCGTGCATGGTCAGGTCGCCAGTCACGTCAGCCGTCTTGTCGCCAGTAGACTTGACGCTGGTGGAGACGAATTTGGCGT  >  AE015451/5674466‑5675043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑