Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451	499,203	A→G	intergenic (+47/‑249)	uhpA → / → PP_0411	DNA‑binding response regulator UhpA/Polyamine ABC transporter, ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	499,203	0	A	G	100.0%	223.1 / NA	60	intergenic (+47/‑249)	uhpA/PP_0411	DNA‑binding response regulator UhpA/Polyamine ABC transporter, ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/30);  total (30/30)

CGCTGGCCGACCGCATGGTGTTCGAAGTAGGCCTCACCGAATCCCGGCCGTTGCACACACTGCTCTCGGAGCGGGAATTTTCGGTGTTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCCATCCGGATGATGGCATTCTCTCAGTACCCCCGATTTTCGGGCGCTTGCGTCTCTTCTACTCTTTTGCCTACGCAGTCATCCAACAAGAAGGTGCAGGCATGAGCGAGGCGAAGTCGAACGCTGCAACCGGCGAAACGCTGGTC  >  AE015451/498909‑499496                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                      cctacacgacgctcttccgatctgAAGTAGGCCTCACCGAATCCCGGCCGTTGCACACACTGCTCTCGGAGCGGGAATTTTCGGTGTTCGAGCCCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATg                                                                                                                                                                                                                                                                                                 <  1:86864/278‑1 (MQ=255)  gCTGGCCGACCGCATGGTGTTCGAAGTAGGCCTCACCGAATCCCGGCCGTTGCACACACTGCTCTCGGAGCGGGAATTTTCGGTGTTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGc                                                                                                                                                                                                                                                                                                >  4:762338/1‑301 (MQ=255)    tGGCCGACCGCATGGTGTTCGAAGTAGGCCTCACCGAATCCCGGCCGTTGCACACACTGCTCTCGGAGCGGGAATTTTCGGTGTTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGccc                                                                                                                                                                                                                                                                                              >  3:348574/1‑301 (MQ=255)     ggCCGACCGCATGGTGTTCGAAGTAGGCCTCACCGAATCCCGGCCGTTGCACACACTGCTCTCGGAGCGGGAATTTTCGGTGTTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcg                                                                                                                                                                                                                                                                                             <  2:706330/301‑1 (MQ=255)                  gttttCGAAGTAGGCCTAACCGAATCCCGGCCGTTGCACACACTGCTCTCGGAGCGGGAATTTTCGGTGTTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcctgg                                                                                                                                                                                                                                                                                <  2:762059/298‑1 (MQ=255)                        gAAGTAGGCCTCACCGAATCCCGGCCGTTGCACACACTGCTCTCGGAGCGGGAATTTTCGGTGTTCGAGCCCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATgagatcggaagagcacacgtctga                                                                                                                                                                                                                                                                          >  3:86867/1‑278 (MQ=255)                           gTAGGCCTCACCGAATCCCGGCCGTTGCACACACTGCTCTCGGAGCGGGAATTTTCGGTGTTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGAt                                                                                                                                                                                                                                                                       >  4:440295/1‑301 (MQ=255)                                  tCACCGAATCCCGGCCGTTGCACACACTGCTCTCGGAGCGGGAATTTTCGGTGTTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATg                                                                                                                                                                                                                                                                <  4:454450/301‑1 (MQ=255)                                      cGAATCCCGGCCGTTGCACACACTGCTCTCGGAGCGGGAATTTTCGGTGTTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTg                                                                                                                                                                                                                                                            >  3:448449/1‑301 (MQ=255)                                        ctcttccgatcttGCACACACTGCTCTCGGAGCGGGAATTTTCGGTGTTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAg                                                                                                                                                                                                                                                          <  4:704318/290‑1 (MQ=255)                                         aTCCCGGCCGTTGCACACACTGCTCTCGGAGCGGGAATTTTCGGTGTTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGACCGCGGACGGGCCTTGCCTGGTGATCTCCATGGCttgac                                                                                                                                                                                                                                                         >  2:595940/1‑297 (MQ=255)                                           tctggCCGTTGCACACACTGCTCTCGGAGCGGGAATTTTCGGTGTTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCg                                                                                                                                                                                                                                                       <  1:165988/298‑1 (MQ=255)                                              ggCCGTTGCACACACTGCTCTCGGAGCGGGAATTTTCGGTGTTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCgaga                                                                                                                                                                                                                                                    >  3:165990/1‑298 (MQ=255)                                               gCCGTTGCACACACTGCTCTCGGAGCGGGAATTTTCGGTGTTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGGTAACGGGCCTGGCCTTCTGATCTCTATGGGTGAGCCgtgcc                                                                                                                                                                                                                                                   >  2:404270/1‑297 (MQ=255)                                                   ttGCACACACTGCTCTCGGAGCGGTAAGTTTCGGTGTTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTc                                                                                                                                                                                                                                               <  1:448447/301‑1 (MQ=255)                                                    tGCACACACTGCTCTCGGAGCGGGAATTTTCGGTGTTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTcg                                                                                                                                                                                                                                              >  1:450433/1‑301 (MQ=255)                                                    tGCACACACTGCTCTCGGAGCGGGAATTTTCGGTGTTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCGGAgagaacggaaaag                                                                                                                                                                                                                                              >  2:704073/1‑289 (MQ=255)                                                               cgttccgatctggAATTTTCGGTGTTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGccc                                                                                                                                                                                                                                   <  2:458318/290‑1 (MQ=255)                                                                 ctcGGAGCGGGAATTTTCGGTGTTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGc                                                                                                                                                                                                                                 >  4:44984/1‑301 (MQ=255)                                                                  ccGGAGCGGGAATTTTCGGTGTTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCt                                                                                                                                                                                                                                <  2:440181/300‑1 (MQ=255)                                                                   cGGAGCGGGAATTTTCGGTGTTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTc                                                                                                                                                                                                                               >  4:733017/1‑301 (MQ=255)                                                                       gCGGGAATTTTCGGTGTTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCcac                                                                                                                                                                                                                           <  3:450435/301‑1 (MQ=255)                                                                          ggAATTTTCGGTGTTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGcccagatcggaagg                                                                                                                                                                                                                        >  4:458447/1‑290 (MQ=255)                                                                                 tCGGTGTTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTg                                                                                                                                                                                                                 <  3:383992/301‑1 (MQ=255)                                                                                         cGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTc                                                                                                                                                                                                         <  4:596137/301‑1 (MQ=255)                                                                                          gAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTcc                                                                                                                                                                                                        >  4:174878/1‑300 (MQ=255)                                                                                          gAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCg                                                                                                                                                                                                        >  4:435022/1‑301 (MQ=255)                                                                                                cTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAggg                                                                                                                                                                                                  >  1:195072/1‑301 (MQ=255)                                                                                                     ccAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTc                                                                                                                                                                                             <  4:404367/301‑1 (MQ=255)                                                                                                           cgcCAACGTAAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCAGCAGCCCCCACAAGGAACGCCTGATGCAGAAGTTGAAAGTGAACGCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATcc                                                                                                                                                                                       <  1:348573/301‑1 (MQ=255)                                                                                                            acaaacttCAACGAAACCGGCCAGCAGTTGGCGCTGTCGAGCAAGACCAGCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATccc                                                                                                                                                                                      <  2:174836/294‑1 (MQ=255)                                                                                                               aaCGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATTCCCGCTCCCACAGGTTTCGGGGTTGGCTCGCAAGGGCATTCCAATCCCGcc                                                                                                                                                                                   >  1:565185/1‑301 (MQ=255)                                                                                                                     aaCGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGAACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCtt                                                                                                                                                                             <  1:192695/301‑1 (MQ=255)                                                                                                                           aTCGCCCAGCAGTTGGCGCTGACGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAggg                                                                                                                                                                       >  4:516282/1‑301 (MQ=255)                                                                                                                            ccgcccagaatgtGGCGCTGTCGGGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGc                                                                                                                                                                      <  2:434908/289‑1 (MQ=255)                                                                                                                            ccGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGc                                                                                                                                                                      <  3:565187/300‑1 (MQ=255)                                                                                                                                        gggCGCTGTGGAGCAAGACCATCAGCACCCACAAGGCACTCCTGATGCAGTAGTTGAAAGTGAACGCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTacaac                                                                                                                                                          <  2:44967/300‑1 (MQ=255)                                                                                                                                                 ggAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCTCCATCCTTGTAGGGCGATCCCTACAACAAATCTTcc                                                                                                                                                 <  2:732757/300‑1 (MQ=255)                                                                                                                                                     cAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAACTCCTCCATcc                                                                                                                                             >  2:153420/1‑301 (MQ=255)                                                                                                                                                           cATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCCATCCgtctgc                                                                                                                                       >  2:582442/1‑296 (MQ=255)                                                                                                                                                            ttCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAATTACGCCATGTAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCCATCCGgatgat                                                                                                                                      <  3:195070/300‑1 (MQ=255)                                                                                                                                                                     tctaaGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCCATCCGGATGATGGCATtctc                                                                                                                             <  1:614445/298‑1 (MQ=255)                                                                                                                                                                        gctcttccgatcttGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCCATCCGGATGATGGCATTCTCTCa                                                                                                                          <  1:275895/288‑1 (MQ=255)                                                                                                                                                                        aaGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCCATCCGGATGATGGCATtctcaga                                                                                                                          >  3:614447/1‑298 (MQ=255)                                                                                                                                                                               gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCCATCCGGATGATGGCATTCTCTCAGTAcccc                                                                                                                   <  4:153458/301‑1 (MQ=255)                                                                                                                                                                                   gATGCAGAAGTTGAAAGTGAACTCGGTGGCGGAGCTGGTGAAGTACGACATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCCATCCGGATGATGGCATTCTCTCAGTACCCCCGAt                                                                                                               <  4:619382/301‑1 (MQ=255)                                                                                                                                                                                     tGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCCATCCGGATGATGGCATTCTCTCaagatcggaagagc                                                                                                             >  3:275894/1‑288 (MQ=255)                                                                                                                                                                                      gcgaacagtGAAAGTGAACTCGCTGGCGGAGCTGTGGAAGTACGCCATGGAGCACAAGATGAGCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCCATCCGGATGATGGCATTCTCTCAGTACCCCCGAtttt                                                                                                            <  2:516132/293‑1 (MQ=255)                                                                                                                                                                                               gAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCCATCCGGATGATGGCATTCTCTCAGTACCCCCGATTTTCGGgccctc                                                                                                   >  1:505313/1‑297 (MQ=255)                                                                                                                                                                                                 ccagacgtgtgctcttccgatctgTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCCATCCGGATGATGGCATTCTCTCAGTACCCCCGATTTTCGGGCGCTTGc                                                                                                 <  3:331227/278‑1 (MQ=255)                                                                                                                                                                                                        cTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATCCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTCTGGCTATCCCTACAACACAGCTTCCAACCTGATGATGGCATTCCCCCAGTACCCCTGGTTTTCGTGCGCtttcgctcctt                                                                                          >  2:4606/1‑292 (MQ=255)                                                                                                                                                                                                                   ggCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCCATCCGGATGATGGCATTCTCTCAGTACCCCCGATTTTCGGGCGCTCGCGTCTCTTCTACTCTTTTg                                                                               <  3:505315/300‑1 (MQ=255)                                                                                                                                                                                                                        gTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTTTAGGGCGCTCCCTACAACAAATCTTCCATCCGGATGATGGCATTCTCTCAGTACCCCCGATTTTCGGGCTCTTGcagatcggaagagcgtcgtgtagg                                                                          >  1:331226/1‑278 (MQ=255)                                                                                                                                                                                                                        gTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCCATCCGGATGATGGCATTCTCTCAGTACCCCCGATTTTCGGGCGCTTGCGTCTCTTCTACTCTTTTGCCTAc                                                                          <  4:582627/301‑1 (MQ=255)                                                                                                                                                                                                                           aaGTAGGCCGTGGAGCAAAAGAGGATCTGATTGCGACAGACGGGTGCGCGAAACTTGGCGAAGCCTATGTGGGAGCGGGCATGCCCGGAAACGGGCCTGGCCTGCTGATCTCTAGGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTGGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCCATCCGGATGATGGCATTCTCTCAGTACCCCCGATTTTCGGGCGCTTGCGTCTCTTCTACTCTTTTGCCTACGCa                                                                       <  4:4606/301‑1 (MQ=255)                                                                                                                                                                                                                                                 tgatctgatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCCATCCGGATGATGGCATTCTCTCAGTACCCCCGATTTTCGGGCGCTTGCGTCTCTTCTACTCTTTTGCCTACGCATTCATCCAACcagaaagtcgag                                                 >  2:142282/1‑294 (MQ=255)                                                                                                                                                                                                                                                                                  tGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCCATCCGGATGATGGCATTCTCTCAGTACCCCCGATTTTCGGGCGCTTGCGTCTCTTCTACTCTTTTGCCTACGCAGTCATCCAACAAGAAGGTGCAGGCATGAGCGAGGCGAAGTCGAACGCTGCAACCg                >  4:23583/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                         gCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCCATCCGGATGATGGCATTCTCTCAGTACCCCCGATTTTCGGGCGCTTGCGTCTCTTCTACTCTTTTGCCTACGCAGTCATCCAACAAGAAGGTGCAGGCATGAGCGAGGCGAAGTCGAACGCTGCAACCGGCGAAAc         >  1:520544/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                           cTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCCATCCGGATGATGGCATTCTCTCAGTACCCCCGATTTTCGGGCGCTTGCGTCTCTTCTACTCTTTTGCCTACGCAGTCATCCAACAAGAAGGTGCAGGCATGAGCGAGGCGAAGTCGAACGCTGCAACCGGCGAAACGc       >  4:261968/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                                tgGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCACGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTCCAACAAATCTTCCATCCGGATGATGGCATTCTCTCAGTACACACGATTTTCGGGCGCTTGCGTCTCTTCTACTCTTTTGCCTACGCAGTCATCCAACAACAAGTTTCAGGCCTGAGCGAGGCCAAGGCGAACGCTGCAACCGGCGACCCGCTGGTc  >  2:66912/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                      CGCTGGCCGACCGCATGGTGTTCGAAGTAGGCCTCACCGAATCCCGGCCGTTGCACACACTGCTCTCGGAGCGGGAATTTTCGGTGTTCGAGCGCCTGGCCCAGGGCGCCAACGTCAACGACATCGCCCAGCAGTTGGCGCTGTCGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCCATCCGGATGATGGCATTCTCTCAGTACCCCCGATTTTCGGGCGCTTGCGTCTCTTCTACTCTTTTGCCTACGCAGTCATCCAACAAGAAGGTGCAGGCATGAGCGAGGCGAAGTCGAACGCTGCAACCGGCGAAACGCTGGTC  >  AE015451/498909‑499496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑