Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451	4,586,035	+G	intergenic (+118/+97)	PP_4061 → / ← PP_4063	conserved protein of unknown function/putative Long‑chain‑fatty‑acid‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	4,586,033	1	.	G	100.0%	100.7 / NA	28	intergenic (+116/+99)	PP_4061/PP_4063	conserved protein of unknown function/putative Long‑chain‑fatty‑acid‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (11/17);  total (11/17)

TCCACCGGAGACACCCGATCCCAATATCGACGACCCGAGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCAGGG‑A‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTGCGATGCGTGCCTTGCACCAGCCCTGCAGCTCCTCGACCGTGGCGCTGTGCCCCG  >  AE015451/4585770‑4586313                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                           cccACCGGAGACACCCGATCCCAATATCGACGACCCGAGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCtt                                                                                                                                                                                                                                                       <  3:524893/300‑1 (MQ=255)    ccacgagcccacccatcgcAATAGCGAGGACCCGGGCTGGCGGGCACCCAGACCGGAAGAAGAGCCGGTCGAGCTGACGAGAAAGCCGACAGGGCGGCGGAAAGTGGGCGACCCGCCGAGCCAGGAGCAAACGGTGAAGGCGTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGaa                                                                                                                                                                                                                                                    <  2:717778/283‑1 (MQ=255)        ctctcacCCGATCCCAATATCGACGACCCGAGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATgcg                                                                                                                                                                                                                                                <  1:639254/297‑1 (MQ=255)            cacCCGATCCCAATATCGACGACCCGAGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATgcgagaa                                                                                                                                                                                                                                            >  3:639267/1‑297 (MQ=255)                  aTCCCAATATCGACGACCCGAGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCAACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGGAAACCAGCTCCTACAAGGG‑ACCGTGCCGGCCTTGAATGCGGCCTTGcccc                                                                                                                                                                                                                                      >  2:772164/1‑301 (MQ=255)                    ttccctacacgacgctcttccgatctgccACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTg                                                                                                                                                                                                                                    <  1:167104/275‑1 (MQ=255)                     ccactacccccggcccGGGCTTCCCCCCACCCGTCCCGGAGGAGGACCCGGACGAACTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGGCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTgg                                                                                                                                                                                                                                   <  2:673091/288‑1 (MQ=255)                                      gccctGCCGCCCCCCGTACCGGAAAAAGACCCGGACGAGCTGCCGATCAAGCCGACCGGGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTAccc                                                                                                                                                                                                                  <  4:625283/297‑1 (MQ=255)                                      ctcTTGCCGCCACCCGTACCGGAAGAAGACCCGGAACAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCAACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGCC‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTAccc                                                                                                                                                                                                                  <  4:772427/299‑1 (MQ=255)                                       gctggccgccACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCcg                                                                                                                                                                                                                 <  4:530435/297‑1 (MQ=255)                                             cgccACCCGTACCGGAAGAAGACCCGGGCGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAaga                                                                                                                                                                                                           <  4:341171/301‑1 (MQ=255)                                              gccACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTgagatcggaagagcacacgtctgaact                                                                                                                                                                                                          >  3:167109/1‑275 (MQ=255)                                                          ggAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCTGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGcc                                                                                                                                                                                              >  3:510500/1‑301 (MQ=255)                                                              gaagaCGCGGACGAGCTGCCGATCAAGCCGACCGTGCCGACGACAGTGGGCGACCCGCCGAGCCGGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGca                                                                                                                                                                                          <  4:370259/301‑1 (MQ=255)                                                                                                          aGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCCCCGCCACACTGAtctc                                                                                                                                              >  4:792551/1‑301 (MQ=255)                                                                                                                                gAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCTGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCAcc                                                                                                                        <  1:510488/301‑1 (MQ=255)                                                                                                                                             aGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACACGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGGCTGCGGAACTTCTGCACCTTGCcgggccagg                                                                                                           >  2:671914/1‑295 (MQ=255)                                                                                                                                                                  cACCGGCGTGAAAAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGTTCTTGAATGGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACaa                                                                                      <  4:672149/301‑1 (MQ=255)                                                                                                                                                                               aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATAccc                                                                         >  3:561935/1‑300 (MQ=255)                                                                                                                                                                                    ttCTGCGCTGGTCCATTGAACGTTTGGGGATGCCCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCa                                                                    <  2:792281/300‑1 (MQ=255)                                                                                                                                                                                                 cATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGtgtgg                                                       >  1:97935/1‑300 (MQ=255)                                                                                                                                                                                                     gAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTAAATGCGGCTCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCCATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGtatataagatcggaagagcacacgtctgaac                                                   >  4:403852/1‑276 (MQ=255)                                                                                                                                                                                                       aaGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACCAAACGGATATCACGCGGCCCCTTGCAGTGTTCGATGcc                                                 >  2:317426/1‑300 (MQ=255)                                                                                                                                                                                                               ggATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTGCGATGCGTGCCTTGc                                         <  4:867116/301‑1 (MQ=255)                                                                                                                                                                                                                     ccGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTGCGATGCGTGCCTTGCACCAGc                                   <  3:97937/301‑1 (MQ=255)                                                                                                                                                                                                                         gTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTGCGATGCGTGCCTTGCACCAGCCCTg                               >  4:32756/1‑301 (MQ=255)                                                                                                                                                                                                                                   gggtaaagttCTTGCCTTGATCGGCCCGGCCGCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGGGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTGCGATGCGTGCCTTGCACCAGCCCTGCAGCTCCTCg                     <  2:32742/292‑1 (MQ=255)                                                                                                                                                                                                                                                      tccGGGCCGGCCTCTTCGCGGGTAACCCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTGCGATGCGTGCCTTGCACCAGCCCTGCAGCTCCTCGACCGTGGCGCTGTGCCCCg  <  4:317507/299‑1 (MQ=255)                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                           TCCACCGGAGACACCCGATCCCAATATCGACGACCCGAGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCAGGG‑A‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTGCGATGCGTGCCTTGCACCAGCCCTGCAGCTCCTCGACCGTGGCGCTGTGCCCCG  >  AE015451/4585770‑4586313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑