New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AE015451 = 48921130 (0.000)4 (0.040)
+GCGGC		 4/590 8.3 21.9% coding (1275/1275 nt)
coding (4/1416 nt)		 ttuD
pyk		 putative hydroxypyruvate reductase
Pyruvate kinase
?AE015451 4893627 = 29 (0.270)intergenic (+102/‑106) pyk/PP_4302 Pyruvate kinase/Urea transporter
Rejected: Coverage evenness skew score above cutoff.

AGTTCCTGCTCAGCCTCACCGAAAGCCTGAAAGGCCTGCCGGGCGTGTACGCCCTGGCCGGTGACACCGACGGCATCGATGGCTCGGAAGAAAACGCCGGTGCCTTCATGACCCCGGCCAGCTACGCCAGCGCCGAAGCCTTGGGCCTGTCGGCCAGCGACGAGCTGGACAACAACAACGGCTACGGCTACTTCGCCGCGCTGGATGCGCTGATCGTCACCGAACCGACCCGCACCAACGTCAACGACTTCCGCGCCATCCTGATCCTTGAGACTGCCCAATCATGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/4891827‑4892113
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGCGATTGGAGGGCAAAGCCCTCCCTGAATACCGCTCTACCCGACCGCGGAAAACCGAGGCCCAAAGAGGCCCACCGCTCCCCCATCAACCTCCACTGACTGCCCCATGTACACCAAAAATTTCGTCAACCCGTGCCCCGACTGGGCCACGGCGTTACTCAACGGCTTCAGCCAGGTGCTGCTGCTGCGCAACCCCCTGTGCGGCCTGTGCTGCC  >  AE015451/4893627‑4893841
                                                                                                                                                                                                                                                                                               |||||                                                                                                                                                                                                                       
ccacgactctcttccgatctGAAAGCCTGAAAGGCCTGCCGGGCGTGTACGCCCTGGCCGGTGACACCGACGGCATCGATGGCTCGGAAGAAAACGCCGGTGCCTTCATGACCCCGGCCAGCTACGCCAGCGCCGAAGCCTTGGGCCTGTCGGCCAGCGACGAGCTGGACAACAACAACGGCTACGGCTACTTCGCCGCGCTGGATGCGCTGATCGTCACCGAACCGACCCGCACCAACGTCAACGACTTCCGCGCCATCCTGATCCTTGAGACTGCCCAATCATGAGCGGCCGCGATTGG                                                                                                                                                                                                                <  1:709823/281‑1
                    GAAAGCCTGAAAGGCCTGCCGGGCGTGTACGCCCTGGCCGGTGACACCGACGGCATCGATGGCTCGGAAGAAAACGCCGGTGCCTTCATGACCCCGGCCAGCTACGCCAGCGCCGAAGCCTTGGGCCTGTCGGCCAGCGACGAGCTGGACAACAACAACGGCTACGGCTACTTCGCCGCGCTGGATGCGCTGATCGTCACCGAACCGACCCGCACCAACGTCAACGACTTCCGCGCCATCCTGATCCTTGAGACTGCCCAATCATGAGCGGCCGCGATTGGAGatcggaagagcaaacgtc                                                                                                                                                                                            >  3:709839/1‑283
                                                                                                                                                                                        ctcaccgacgctcttccgatcTGCGCTGACCGTCACCGAACCGACCCGCACCAACGTCAACGACTTCCGCGCCATCCTGATCCTTGAGACTGCCCAATCATGAGCGGCCGCGATTGGAGGGCAAAGCCCTCCCTGAATACCGCTCTACCCGACCGCGGAAAACCGAGGCCCAAAGAGGCCCACCGCTCCCCCATCAACCTCCACTGACTGCCCCATGTACACCAAAAATTTCGTCAACCCGTGCCCCGACTGGGCCACGGCGTTACTCAACGGCTTCAGCCAGGTGCTGCTGCTGCGCAAC                        <  1:602510/280‑1
                                                                                                                                                                                                              GCGCTGATCGTCACCGAACCGACCCGCACCAACGTCAACGACTTCCGCGCCATCCTGATCCTTGAGACTGCCCAATCATGAGCGGCCGCGATTGGAGGGCAAAGCCCTCCCTGAATACCGCTCTACCCGACCGCGGAAAACCGAGGCCCAAAGAGGCCCACCGCTCCCCCATCAACCTCCACTGACTGCCCCATGTACACCAAAAATTTCGTCAACCCGTGCCCCGACTGGGCCACGGCGTTACTCAACGGCTTCAGCCAGGTGCTGCTGCTGCGCAACagatcggaagagcacacgtctg  >  3:602522/1‑279
                                                                                                                                                                                                                                                                                               |||||                                                                                                                                                                                                                       
AGTTCCTGCTCAGCCTCACCGAAAGCCTGAAAGGCCTGCCGGGCGTGTACGCCCTGGCCGGTGACACCGACGGCATCGATGGCTCGGAAGAAAACGCCGGTGCCTTCATGACCCCGGCCAGCTACGCCAGCGCCGAAGCCTTGGGCCTGTCGGCCAGCGACGAGCTGGACAACAACAACGGCTACGGCTACTTCGCCGCGCTGGATGCGCTGATCGTCACCGAACCGACCCGCACCAACGTCAACGACTTCCGCGCCATCCTGATCCTTGAGACTGCCCAATCATGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/4891827‑4892113
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGCGATTGGAGGGCAAAGCCCTCCCTGAATACCGCTCTACCCGACCGCGGAAAACCGAGGCCCAAAGAGGCCCACCGCTCCCCCATCAACCTCCACTGACTGCCCCATGTACACCAAAAATTTCGTCAACCCGTGCCCCGACTGGGCCACGGCGTTACTCAACGGCTTCAGCCAGGTGCTGCTGCTGCGCAACCCCCTGTGCGGCCTGTGCTGCC  >  AE015451/4893627‑4893841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.