Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	1,124,554	1	.	T	39.5%	19.4 / 73.3	43	coding (589/1122 nt)	gcvT‑I	aminomethyltransferase
Reads supporting (aligned to +/- strand):  ref base . (12/14);  new base T (17/0);  total (29/14)
Fisher's exact test for biased strand distribution p-value = 1.50e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CTGCGGCAGCAGGCCGACACGTTTGCGCGCAACACCATCGCGCACGTGGGCGAAGATCGCCTCGGCACCCGGGAAGCCGGCAGCACGTGCACCGTCGGCACGGCGCACC‑TTGGAAATTGCCCACAGCAGGCTGGCTTCGATCGGCGTAGTCTCGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTA‑GCCGTCTTCGCCGGTG‑TAGCCCGAGCGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAGCCAGGCGTTCCAGCACCTTGACTGCAGCCGGGCCTTGCAGGGCGAGCAGGGCACGCTCCTCGAACAGCGGCTGCACCTCGCAACGGCTGCCGATGTGGGTTTGCAGGTGAGCCAGGTCCTGCTCCTTGCAGGCGGCGTTGACGACCAGGAACAGGGTGTCCTCGCCCAGGTTGGCAACCATCAGGTCGTCGAGAAT  >  AE015451/1124255‑1124851                                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                           cggcggacgcagGCCGAACCGTTGGCCCGCACAACCATGGCGAACGTGGGCGAAGATCGCCTCGGAACCCGGGAAGCCGGCAGCACGTGCACCGTCGGCACGGCGCACC‑TTGGAAATTGCCCACAGCAGGCTGGCTTCGATCGGCGTAGTCGCGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTA‑g                                                                                                                                                                                                                                                                                                           <  2:348409/293‑1 (MQ=255)     ggCAGCAGGCCGACACGTTTGCGCGCAACACCATCGCGCACGTGGGCGAAGATCGCCTCGGCACCCGGGAAGCCGGCAGCACGTGCACCGTCGGCACGGCGCACC‑TTGGAAATTGCCCACAGCAGGCTGGCTTCGATCGGCGTAGTCTCGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTatgccg                                                                                                                                                                                                                                                                                                        >  3:713392/1‑296 (MQ=255)             gCCGACACGTTTGCGCGCAACACCATCGCGCACGTGGGCGAAGATCGCCTCGGTACCCGGGAAGCCGGCAGCACGTGCACCGTCGGCACGGCGCACC‑TTGGAAATTGCCCACAGCAGGCTGGCTTCGATCGGCGTAGTCTCGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTTACCGGTACCGAGATCTCGTA‑GCCGTCTTAGccc                                                                                                                                                                                                                                                                                               >  2:733526/1‑300 (MQ=255)                  cactgttGCGCGCACAACCATCGCGCACGTGGGCGACGATCGCCTCGGTACCCGGGAGGCCGGCAGCACGTGCACCGTCGGCACGGCGCACC‑TTGGAAATTGCCCACAGCAGGCTGGCTTCGATCGGCGTAGTCTCGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTA‑GCCGTCTTCGCCGGTG‑Ta                                                                                                                                                                                                                                                                                         <  4:733774/296‑1 (MQ=255)                                       cgcgCACGTGGGCGAAGATCGCCTCGGCACCCGGGAAGCCGGCAGCACGTGCACCGTCGGCACGGCGCACC‑TTGGAAATTGCCCACAGCAGGCTGGCTTCGATCGGCGTAGTCTCGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTATGCCGTCTTccgcttgaaaaacaaacgactgacgcagca                                                                                                                                                                                                                                                                      >  3:218212/1‑272 (MQ=255)                                                        ctcgctcccgaacccgggaacacgcaagcaCGTGCACCTTCGGCACGGCGAACC‑TTGGAAACTTCCCACAGCAGGCCGGCTTCGATCGGCGTAGTCTGGCTTTTCATGTCGTGGCCATCCAGGCACAGGCCCGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGTTCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTA‑GCCGTCTTCGCCGGTG‑TAGCCCGAGCGGCTGACGAAGCAGTCCACACCCAGCAGtt                                                                                                                                                                                                                                                   <  1:496974/275‑1 (MQ=255)                                                           tttgcGGCACCAGGGAAGCTGGCAGCACGTGCACGATCGGCACGGCG‑ACCACTGGAAATTGCCTACTGCAGGCTGGTTGGGGTCGGCGTAGTCTCGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTA‑GCCGTCTTCGCCGGTG‑TAGCCCGAGCGGCTGACGAAGCAGTCCACACCCAGCAGTTTTa                                                                                                                                                                                                                                                <  3:615603/297‑1 (MQ=255)                                                              tCGGCACCCGGGAAGCCGGCAGCACGTGCACCGTCGGCACGGCGCACC‑TTGGAAATTGCCCACAGCAGGCTGGCTTCGATCGGCGTAGTCTCGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCGGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTCACCGGTACCGCGATCTCGTA‑TCCGTATTCGCC‑GTGTTACCCCGAGCGGCTGACGAAGCCGTCCAAACCCAGGCGTTTTaagt                                                                                                                                                                                                                                             >  1:615591/1‑298 (MQ=255)                                                                   aCCCGGGAAGCCGGCAGCACGTGCACCGTCGGCACGGCGCACC‑TTGGAAATTGCCCACAGCAGGCTGGCTTCGATCGGCGTAGTCTCGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTA‑GCCGTCTTCGCCGGTG‑TAGCCCGAGCGGCTGACGAAGCAGTCCACACCCAGCAGttttcgatcggaa                                                                                                                                                                                                                                        >  1:380919/1‑292 (MQ=255)                                                                               gttcagacgttgtgctcttccgatctgcACC‑TTGGAACTGGCCCACAGCAGGCTGGCTTCGATCGGCGTAGTCTCGCTGTTCATGTCGTGCACATACAGGCACATGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTA‑GCCGTCTTCGCCGGTG‑TAGCCCGAGCGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAg                                                                                                                                                                                                                            <  4:596006/275‑1 (MQ=255)                                                                                       gTGCACCGTCGGCACGGCGCACC‑TTGGAAATTGCCCACAGCAGGCTGGCTTCGATCGGCGTAGTCTCGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTATGCCGTCTTCTGC‑TTG‑AAAAAAAAACAGCTGACGAAGCAGTACAAACCCAGCAGTTTTACCCGGCGAAACTGCATGCAGGTCATGcc                                                                                                                                                                                                                    >  3:718156/1‑301 (MQ=255)                                                                                       cggcactgccggaccGGCGCACC‑TTGAAAATGGCCCACAGCAGGCTGGTTTCTGGCGGCGGAGTCTCGCGGTCCATGTCGTGGCCCTACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCACGCCGGTCGGCTGTACTTCCGGCTCGGCCATCTGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTA‑GCCGTCTTCGCCGGTG‑TAGCCCGAGCGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGcc                                                                                                                                                                                                                    <  2:568143/287‑1 (MQ=255)                                                                                         gCACCGTCGGCACGGCGCACC‑TTGGAAATTGCCCACAGCAGGCTGGCTTCGATCGGCGTAGTCTCGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTATTCGGCCTTC‑CCGGTG‑TAGCCCGAACGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCgg                                                                                                                                                                                                                  >  3:255857/1‑301 (MQ=255)                                                                                                  gCACGGCGCACC‑TTGGAAATTGCCCACAGCAGGCTGGCTTCGATCGGCGTAGTCTCGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTATGCCGTCTTCTGC‑TTG‑AAAAAAAAAAGGCTGACGAAGCAGTCCACACCCAGAAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCAGGCCACTTCTg                                                                                                                                                                                                         >  4:490208/1‑301 (MQ=255)                                                                                                         ccACC‑TTGGAAATTGCCCACAGCAGGCTGGCTTCGATCGGCGTAGTCTCGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTA‑GTCGTCTTCGCCGGTG‑TAGCCCGAGCGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAGCCAg                                                                                                                                                                                                  <  1:255852/300‑1 (MQ=255)                                                                                                           aCC‑TTGGAAATTGCCCACAGCAGGCTGGCTTCGATCGGCGTAGTCTCGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGTCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTA‑GCCGTCTTCGCCGGTG‑TAGCCCGAGCGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAGCCAggg                                                                                                                                                                                                >  1:195575/1‑300 (MQ=255)                                                                                                                                 agGCTGGCTTCGATCGGCGTAGTCTCGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTATGCCGTCTTCTGC‑TTG‑TAAAAAAAACAGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGCACTGCATGAAGGTCATGCCGGCCCCTTCTGGAGACAGGCGGTCCAGCCCCGTGACTGCAg                                                                                                                                                                           >  4:323713/1‑301 (MQ=255)                                                                                                                                   gctggctTCGATCGGCGTAGTCTCGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTATCCCGTCTTC‑CGCTTG‑TAGAACAAAAAGCTGACGAAGCAGACCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAGCCAGGCGTTCCAGCACCTTGACTGAAGcc                                                                                                                                                                         >  4:198593/1‑301 (MQ=255)                                                                                                                                   cctggctgccctcggcgtctttgcgcggTTCATGTGGTGTCCATACTGGCACAGTGCAGATTCCAGGCGCAGACAGTCGCGTGCGCCCCAGCCGAGCGGCTGTACTTCCGGCTCCGCCAGCAGGCGGCGGGCCAAGGCGTCGGCGGCGTTGACCGGTACGGAGATCTCGTA‑GCCGTCGTCGCCGGTG‑TAGCCCGAGCGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAGCCAGGCGTTCCAGCACCTTGACTGCAGcc                                                                                                                                                                         <  1:718140/274‑1 (MQ=255)                                                                                                                                      ggctTCGATCGGCGTAGTCTCGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTATGCCGTCTTCTGC‑TTG‑AAAAAAAAAACGCTGACGAATAAGTCCACACCCAGCACTTTTACCCCGCTGACCTGCATGAAGGTACTGCCCGCCACTTCTGCAATCTGGAGTTCCCGCACCTCGACTACAGCCggg                                                                                                                                                                      >  3:727742/1‑301 (MQ=255)                                                                                                                                              tCGGCGTAGTCTCGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTATGCCGCCTTCTGC‑TTG‑TAAAAAAAAAGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCAGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGCGCCAGGCGTTCCAGCACCTTGACTTCAGCCGGGCCGTGCAg                                                                                                                                                              >  4:757896/1‑301 (MQ=255)                                                                                                                                                        ctcGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTATGCCGTCTTCTGC‑TTG‑AAAAAAAAAAAGCATAAGAAGCAGTCCACACCCCGCAGTTTTACCCCGCGGAACTGCCAGAATGTCCTGCCGGCAACTTCTGCAGCCCGGCCTTCCAGCACCTTGACTGCAGCCGTGCCTTTCCGGGCGacagat                                                                                                                                                    >  4:546813/1‑296 (MQ=255)                                                                                                                                                        ctcGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTATGCCGTCTTCTGC‑GTG‑TAAAAAAAAAGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAGCCAGGCGGTCCAGCACCTTGACTGCAGCCGGGCCTTGCAGGGCGAGCAgg                                                                                                                                                    >  3:329247/1‑301 (MQ=255)                                                                                                                                                            tggaggtcagacgtgtgatcgtccgatctgcCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTA‑GCCGTCTTCGCCGGTG‑TAGCCCGAGCGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAGCCAGGCGTTCCAGCACCTTGACTGCAGCCGGGCCTTGCAGGGCGAGCAGGGCAc                                                                                                                                                <  4:796536/272‑1 (MQ=255)                                                                                                                                                            cTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTATGCCGTTTTC‑CCCTTG‑TAGACCAAAAGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAGCCAGGCGTTCCAGCACCTTGACTGCAGCCGGGCCTTGCAGGGCGAGCAGGGCAc                                                                                                                                                >  4:698242/1‑301 (MQ=255)                                                                                                                                                                      cGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTATGCCGTCTTCTGC‑TTG‑AAAAAAAAAAGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAGCCAGGCGTTCCAGCACCTTGACTGCAGCCGGGCCTTGCAgatcggaagagcacacgtctgaact                                                                                                                                      >  3:116497/1‑277 (MQ=255)                                                                                                                                                                        tGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTATGCCGTCTCCTGC‑TTG‑AAAACCAAACGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAGCCAGGCGTTCCAGCACCTTGACTGCAGCCGGGCCTTGCAGGGCGAGCAGGGCACGCTCCTCTAAcc                                                                                                                                    >  4:847314/1‑300 (MQ=255)                                                                                                                                                                                  ggcacaggcCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTATGCCGTCTTCTGC‑TTG‑AAAAAAAAAAGGCTGACGAAACAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCCCTTCTGGAGCCAGGCGTTCCAGAACCTTGAATGCAGCCCGGCCTTGCAGGGCGAGCAGGGCCAGCTCCTCGAACAGCAGGTGCAc                                                                                                                          >  3:41659/1‑301 (MQ=255)                                                                                                                                                                                    cacaggcCACCTTCCGGGCTCAGCGAGTCGCTTGCGCCCAAGCCGATCGTCTGTACTTCCGGCTCGGTCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTA‑GCCGTCTTCGCCGGTG‑TAGCCCGAGCGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAGCCAGGCGTTCCAGCACCTTGACTGCAGCCGGGCCTTGCAGGGCGAGCAGGGCACGCTCCTCGAACAGCGGCTGCACCt                                                                                                                        <  3:195582/301‑1 (MQ=255)                                                                                                                                                                                    caaaggcCAGCTTCCATGCGCAGCGAGTGGCGTGCGCCCGAGCAGATCGGCTTTACTTCCGGCTCGGCCAGCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTA‑GCCGTCTTCGCCGGTG‑TAGCCAGAGCGGCTGACGAAGCAGTCCACACCCAGCAGGTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAGCCAGGCGTTCCAGCACCTTGACTGCAGCCGGGCCTTGCAGGGCGAGCAGGGCACGCTCCTCGAACAGCGGCTGCACCt                                                                                                                        <  1:329238/298‑1 (MQ=255)                                                                                                                                                                                      tctgtcctaacacgacgatcttccgttctgTGCGTCCAAGACGATCGGCTGTACTTCCGGCGCGGCCATCATGAGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTA‑GCCGTCTTCGCCGGTG‑TAGCCCGAGCGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAGCCAGGCGTTCCAGCACCTTGACTGCAGCCGGGCCTTGCAGGGCGAGCAGGGCACGCTCCTCGAACAGCGGCTGAACCTCg                                                                                                                      <  2:702156/272‑1 (MQ=255)                                                                                                                                                                                         gcCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTA‑GCCGTCTTCGCCGGTG‑TAGCCCGAGCGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAGCCAGGCGTTCCAGCACCTTGACTGCAGCCGGGCCTTGCAGGGCGAGCAGGGCAcagatcggaagagcgtcgtgtggggaaaga                                                                                                                   >  2:796266/1‑272 (MQ=255)                                                                                                                                                                                           ccttccaatctcgccgCGAGTCGCGTGCGCCCAAGCCGCTCGGCTGTAATTCCGGCGCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTA‑GCCGTCTTCGCCGGTG‑TAGCCCGAGCGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAGCCAGGCGTTCCAGCACCTTGACTGCAGCCGGGCCTTGCAGGGCGAGCAGGGCACGCTCCTCGAACAGCGGCTGCACCTCGCAACg                                                                                                                 <  4:262122/286‑1 (MQ=255)                                                                                                                                                                                                      cgcAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTA‑GCCGTCTTCGCCGGTG‑TAGCCCGAGCGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAGCCAGGCGTTCCAGCACCTTGACTGCAGCCGGGCCTTGCAGGGCGAGCAGGGCACGCTCCTCGAACAGCGGCAGCACCTAGCaaacagatcggaagg                                                                                                      >  2:262053/1‑288 (MQ=255)                                                                                                                                                                                                            gAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTATGCCGTCTTCTGC‑TTG‑TAACAAAAACAGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAGCCAGGCGTTCCAGCACCTTGACTGCAGCCGGGCCTTGCAGGGCGAGCAGGGCACGCTCCTCGAACAGCGGCTGCACCTCGCAACGGCTGCCGATGTGGGttt                                                                                                >  3:230474/1‑301 (MQ=255)                                                                                                                                                                                                                 gcgTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTATGCCGTCTTCTGC‑TTG‑AAAAAAAAAAGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAGCCAGGCGTTCCAGCACCTTGACTGCAGCCGGGCCTTGCAGGGCGAGCAGGGCCCGCTCCTCGAACAGCGGCTGCACCTCGCAACGGCTGCCGATGTGGGTTTGCAgg                                                                                           >  3:39739/1‑301 (MQ=255)                                                                                                                                                                                                                   gTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTA‑TCCGGCCTCGTCGGTG‑TAGAAAGAGCGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAGCCAGGCGTTCCAGCACCTTGACTGCAGCCGGGCCTTGCAGGGCGAGCCGGGCACGCTCCTCGAACAGCGGCTGCACCTCgagatcggacgagcacacgtctgaactcca                                                                                         >  4:702398/1‑272 (MQ=255)                                                                                                                                                                                                                           aaGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTATGCCGTCTTCTGC‑TTG‑AAAAAAAAAAGACTGACGAAGCAGTCCACACCCCGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAGCCAGGCGTTCCAGCACCTTGACTGCAGCCGGGCCTTGCAGGGCGAGCAGGGCACGCTCCTCGAACAGCGGCTGAACCACGCAACGACTGCCGATGTGGGTTTACAGGTGAGCCAggg                                                                                 >  3:612006/1‑300 (MQ=255)                                                                                                                                                                                                                               cGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTA‑TCCCGCTTTCCCCGTG‑TAAACCGAACGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAGCCAGGCGTTCCAGCACCTTGACTGCAGCCGGGCCTTGCAGGGCGAGCAGGGCACGCTCCTCGAACAGCGGCTGCACCTCGCAACGGCTGCCGATGTGGGTTTGCAgatcggaagagcacac                                                                             >  4:712588/1‑286 (MQ=255)                                                                                                                                                                                                                                     gctgtagggacGGCTCGGACATCAGGCGGCGCGCTAAGGCGGTGACAGCGTTGACCGGTACCGAGATCTCGTA‑GCCGTCTTCGCCGGTG‑TAGCCCTATCGGCTGAAGAAGCAGTCCACATCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCCGCCACTTCTGGAGCCAGGCGTTCCAGCACCTTGACTGCATCCGGGCCTTGCAGGGCGGGCAGGGCACGCTCGTCGAACAGCGGCTGCAACTCGCAACGGCTGCCGATGTGGGTTTGCAGGTGAGCCAGGTCCTGCTCCtt                                                                       <  1:39738/291‑1 (MQ=255)                                                                                                                                                                                                                                                       gtgtgcgcttccgatttaaGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTA‑GCCGTCTTCGCCGGTG‑TAGCCCGAGCGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAGCCAGGCGTTCCAGCACCTTGACTGCAGCCGGGCCTTGCAGGGCGAGCAGGGCACGCTCCTCGAACAGCGGCTGCACCTCGCAACGGCTGCCGATGTGGGTTTGCAGGTGAGCCAGGTCCTGCTCCTTGCAGGCGGCGTTgacgac                                                     <  3:366292/284‑1 (MQ=255)                                                                                                                                                                                                                                                                        aaGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTA‑GCCGTCTTCGCCGGTG‑TAGCCCGAGCGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAGCCAGGCGTTCCAGCACCTTGACTGCAGCCGGGCCTTGCAGGGCGAGCAGGGCACGCTCCTCGAACAGCGGCTGCACCTCGCAACGGCTGCCGATGTGGGTTTGCAGGTGAGCCAGGTCCTGCTCCTTGCAGGCGGCGTTgacgacagatcggaagggcgtcg                                    >  1:366282/1‑284 (MQ=255)                                                                                                                                                                                                                                                                         aGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTA‑TCCCTTCTCCCCCGTG‑TAACCCAAGCGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAGCCAGGCGTTCCAGCACCTTGACTGCAGCCGGGCCTTGCAGGGCGAGCAGGGCACGCTCCTCGAACAGCGGCTGCACCTCGCAACGGCTGCCGATGTGGGTTTGCAGGTGAGCCAGGTCCTGCTCCTTGCAGGCGGCGTTTACGACCAGGAACAGGGTGTCCTc                                   >  4:647293/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                    gCGTTGACCGGTACCGAGATCTCGTA‑GCCGTCTTCGCCGGTG‑TAGCCCGAGCGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAGCCAGGCGTTCCAGCACCTTGACTGCAGCCGGGCCTTGCAGGGCGAGCAGGGCACGCTCCTCGAACAGCGGCTGCACCTCGCAACGGCTGCCGATGTGGGTTTGCAGGTGAGCCAGGTCCTGCTCCTTGCAGGCGGCGTTGACGACCAGGAACAGTGTGTCCTCGCCCAGGTTgg                        >  1:408751/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                                          cGTA‑GCCGTCTTCGCCGGTG‑TAGCCCGAGCGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAGCCAGGCGTTCCAGCACCTTGACTGCAGCCGGGCCTTGCAGGGCGAGCAGGGCACGCTCCTCGAACAGCGGCTGCACCTCGCAACGGCTGCCGATGTGGGTTTGCAGGTGAGCCAGGTCCTGCTCCTTGCAGGCGGCGTTGACGACCAGGAACAGGGTGTCCTCGCCCAGGTTGGCAACCATCAGGTCGTCGAGAAt  >  1:22529/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                           CTGCGGCAGCAGGCCGACACGTTTGCGCGCAACACCATCGCGCACGTGGGCGAAGATCGCCTCGGCACCCGGGAAGCCGGCAGCACGTGCACCGTCGGCACGGCGCACC‑TTGGAAATTGCCCACAGCAGGCTGGCTTCGATCGGCGTAGTCTCGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTA‑GCCGTCTTCGCCGGTG‑TAGCCCGAGCGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAGCCAGGCGTTCCAGCACCTTGACTGCAGCCGGGCCTTGCAGGGCGAGCAGGGCACGCTCCTCGAACAGCGGCTGCACCTCGCAACGGCTGCCGATGTGGGTTTGCAGGTGAGCCAGGTCCTGCTCCTTGCAGGCGGCGTTGACGACCAGGAACAGGGTGTCCTCGCCCAGGTTGGCAACCATCAGGTCGTCGAGAAT  >  AE015451/1124255‑1124851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑