Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451	3,951,161	A→T	intergenic (‑125/+70)	PP_3486 ← / ← PP_3487	putative cytochrome c/conserved exported protein of unknown function

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	3,951,161	0	A	T	100.0%	200.0 / NA	59	intergenic (‑125/+70)	PP_3486/PP_3487	putative cytochrome c/conserved exported protein of unknown function
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (30/29);  total (30/29)

AGGGCAGCACGCTGGCGGGGACTGTGATATCGCTGGCGCCGACCCGGGCTTGCAATTGAGCGTCGCTACTGGAGACGCCCTCGCGGTAAAGGCGTTTGCCTGCTTGTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCTTATCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCTGCGTCGAATGCTGCTCGGGACTCGGCAGGAACTGCCCTATACGGCCAAGCACCAGGTCGACATTGGCCTTGTCGCCGGTCAGGAACAGCCAGTTCGGCCCATCCACC  >  AE015451/3950883‑3951453                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                      aGGGCAGCACGCTGGCGGGGACTGTGATATCGCTGGCGCCGACCCGGGCTTGCAATTGAGGGTCGCTACTGGAGACGCCCTCGCGGTAAAGGCGTTTGCCTGCTTGTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCca                                                                                                                                                                                                                                                                                 <  4:867602/301‑1 (MQ=255)         aCGCTGGCGGGGACTGTGATATCGCTGGCGCCGACCCGGGCTTGCAATTGAGCGTCGCTACTGGAGACGCCCTCGCGGTAAAGGCGTTTGCCTGCTTGTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCAGCCTTGCGTCGCGACAGGGCTGCGACGCCGCCCCGGCAGTGGCCGCTCAAGCGCAGGAAGCGGTGAACGCTGCGCGCTCCTTTCACGAAGCAAGGCCGCTACCACCAAAGcc                                                                                                                                                                                                                                                                         >  1:258529/1‑300 (MQ=255)              gccGGGGACGGTGATATCGCTGGTGCCGACCTGGGCTGGCAATGGGGCGTCGCTACGGGAGACGCCTTGGCGGTAAAGGCGTTTGCCTGCTTGTTCGTGTTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGc                                                                                                                                                                                                                                                                    <  3:258534/299‑1 (MQ=255)                                                 cTTGCAATTGAGCGTCGCTACTGGAGACGCCCTCGCGGTAAAGGCGTTTGCCTGCTTGTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACt                                                                                                                                                                                                                                >  4:495923/1‑301 (MQ=255)                                                    gtgctcttccgatcttACTGGAGACGCCCTCGCGGTAAAGGCGTTTGCCTGCTTGTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCac                                                                                                                                                                                                                             <  3:7096/286‑1 (MQ=255)                                                      aaTTGAGCGTCGCTACTGGAGACGCCCTCGCGGTAAAGGCGTTTGCCTGCTTGTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTTaccg                                                                                                                                                                                                                           >  4:30332/1‑301 (MQ=255)                                                         ggggggTCGCTACTGGGGACGCCCTCGCGGTAAAGGCGTTTGCCTGCTTGTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCaccgacc                                                                                                                                                                                                                        <  2:30318/296‑1 (MQ=255)                                                         cccttccgtgctcgggAGACGCCCTCGCGGTAAAGGCGGTTGCCTGGTTGTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGACGTCGAAGTTGCCAGGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCaccgacc                                                                                                                                                                                                                        <  2:432175/287‑1 (MQ=255)                                                              ttCGCTACTGGAGACTCCCTCGCGGTAAAGGCGTTTGCCTGCTTGTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCa                                                                                                                                                                                                                   <  1:406032/300‑1 (MQ=255)                                                              gTCGCTACTGGAGACGCCCTCGCGGTAAAGGCGTTTGCCTGCTTGTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGccc                                                                                                                                                                                                                   >  4:240635/1‑300 (MQ=255)                                                              gTCGCTACTGGAGACGCCCTCGCGGTAAAGGCGTTTGCCTGCTTGTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGccc                                                                                                                                                                                                                   >  3:406043/1‑300 (MQ=255)                                                                   tACTGGAGACGCCCTCGCGGTAAAGGCGTTTGCCTGCTTGTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGCCGCAAGGCCGCTCCCACAAACACGGCTGCCCAAGAGGCCAATTCCCCAGGCCGCCCCTGAtcaccccccaccagccagccgc                                                                                                                                                                                                               >  2:398776/1‑280 (MQ=255)                                                                   tACTGGAGACGCCCTCGCGGTAAAGGCGTTTGCCTGCTTGTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCacagatcggaagagcgg                                                                                                                                                                                                              >  1:7095/1‑286 (MQ=255)                                                                    aCTGGAGACGCCCTCGCGGTAAAGGCGTTTGCCTGCTTGTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCt                                                                                                                                                                                                             >  4:881935/1‑301 (MQ=255)                                                                     gtggggcggcctccgcgGTAAAGCCGTTTGCCTGGTGTTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGCCCAGCGGCTg                                                                                                                                                                                                            <  2:495773/288‑1 (MQ=255)                                                                     cTGGAGACGCCCTCGCGGTAAAGGCGTTTGCCTGCTTGTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGAGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACACaacgaccagaccggaagag                                                                                                                                                                                                            >  4:432298/1‑289 (MQ=255)                                                                      tGGAGACGCCCTCGCGGTAAAGGCGTTTGCCTGCTTGTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAACGGGCTGCGCCGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGGGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGACGATGCCACAGAGGCCAATTCCACAGGCGGCCCCTGCTCAC‑CCCCGACCGGCCAGCGGCTgg                                                                                                                                                                                                           >  1:591604/1‑301 (MQ=255)                                                                         agaCGCCCTCGCGGTAAAGGCGTTTGCCTGCTTGTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGACACAGAGGCTCATTCCCACGACGGCCCCCGCT‑ACCCACCGACCGGcccgactcagggtc                                                                                                                                                                                                        >  2:228052/1‑289 (MQ=255)                                                                              cccTCGCGGTAAAGGCGTTTGCCTGCTTGTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTGGCCAGGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTGGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAg                                                                                                                                                                                                   <  3:591615/301‑1 (MQ=255)                                                                                         aaGGCGTTTGCCTGCTTGTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCt                                                                                                                                                                                        <  4:857879/301‑1 (MQ=255)                                                                                                            tCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCgg                                                                                                                                                                     <  3:126888/301‑1 (MQ=255)                                                                                                                          gTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGGGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGATCGGCCAGCAGCTGGGTAAACCGCTGCATGCGCCGGGCCATcgccggcggcagtgccgccgggctag                                                                                                                                                       >  1:577928/1‑280 (MQ=255)                                                                                                                                 tgctcttccgatctCGAACTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCCAGCCCTGTGGGAGCGGCCTTGCGTCGCGAACGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCt                                                                                                                                                <  4:762669/288‑1 (MQ=255)                                                                                                                                      ggcTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGc                                                                                                                                           >  3:106433/1‑301 (MQ=255)                                                                                                                                       gcTGAGTTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCg                                                                                                                                          <  1:106430/301‑1 (MQ=255)                                                                                                                                        gtgaagTCGAAGTTGCCATGCCAAGCACGCGGGTAAGCCATATCGAAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGt                                                                                                                                         <  4:228106/296‑1 (MQ=255)                                                                                                                                          gtgtgctcttccgatctgccaAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGttt                                                                                                                                       <  3:105855/285‑1 (MQ=255)                                                                                                                                          ccgatctaaGTTGCCATGGCAAGCACGCAGGTAAGACATATCGCAAGCCCTGGGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGGGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGCCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGttt                                                                                                                                       <  4:344712/294‑1 (MQ=255)                                                                                                                                           aGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTa                                                                                                                                      >  4:879519/1‑301 (MQ=255)                                                                                                                                               cGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGGCAATCTTgttagagccgaagagcg                                                                                                                                  >  2:762412/1‑285 (MQ=255)                                                                                                                                                         atgccaAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCCTGGTCAACAGCTGAATGCCCTGGGCAATCGCCGCCGGCCGTGCATCCGGGCCGATCTTTCCCCCGCGTTTAACTGCCACaccacc                                                                                                                        >  2:64249/1‑301 (MQ=255)                                                                                                                                                         atgccaAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCAGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGATGGCGGTGCATCCGGGCGGATGTTGCTCCAGCGTTTACCTGCAACATccac                                                                                                                        >  2:87895/1‑298 (MQ=255)                                                                                                                                                           gccaAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTagatcggaagagcgtcg                                                                                                                      >  1:105852/1‑285 (MQ=255)                                                                                                                                                                 cgcgcgggTAAGCCATCTCGCAAGCCCTGTGGGGGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGAAGTGGCAGCTGAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCa                                                                                                                <  2:240571/295‑1 (MQ=255)                                                                                                                                                                 agcgagggaaagccaaacgtaaaGCCCTGGGGGGGCGGCCTGGCGTCGCGAAAGGGTGGCGCAGCAGCCCCGGAATGGGCAGCTAAAGCGCAGAAAGAGGGGAGCGCTGCGCGCCCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCa                                                                                                                <  2:879207/280‑1 (MQ=255)                                                                                                                                                                    tttccgatctcaaatCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCAAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCt                                                                                                             <  3:476920/288‑1 (MQ=255)                                                                                                                                                                    gCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCt                                                                                                             <  4:87921/301‑1 (MQ=255)                                                                                                                                                                          aaGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGATCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCTGCGTCg                                                                                                       <  4:398889/301‑1 (MQ=255)                                                                                                                                                                              cATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCCAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACCCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTCGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGAGTTTGACTTCAACATCCCCTGCAATCAGccagatcggaaa                                                                                                   >  1:476909/1‑290 (MQ=255)                                                                                                                                                                                tatCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCTGCGTCGAAtgct                                                                                                 <  4:64268/301‑1 (MQ=255)                                                                                                                                                                                               gggAGCGGCCTTGCGTGGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTAAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCTGCGTCGAATGCTGCTCGGGACTCGGCa                                                                                  <  3:197672/301‑1 (MQ=255)                                                                                                                                                                                                        cTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGCCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACAGGCAATCAGCTGCGTCGAATGCTGCTCGGGACTCGGCAGGAACTGcc                                                                         >  4:626838/1‑301 (MQ=255)                                                                                                                                                                                                         ttGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCCTCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACCTCACCGGCCATCAGCTGCATCGAATCCTTCTCCGGACCCGGCAGGAACTTccc                                                                        >  2:3893/1‑301 (MQ=255)                                                                                                                                                                                                             gTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCTGCGTCGAATGCTGCTCGGGACTCGGCAGGAACTGCCCtata                                                                    >  1:597797/1‑301 (MQ=255)                                                                                                                                                                                                             gTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCTGCGTCGAATGCTGCTCGGGACTCGGCAGGAACTGCCCtata                                                                    >  1:163669/1‑301 (MQ=255)                                                                                                                                                                                                               cttccgatctcTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCTGCGTCGAATGCTGCTCGGGACTCGGCAGGAACTGCCCTATACg                                                                  <  4:850121/291‑1 (MQ=255)                                                                                                                                                                                                                         cTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCTGCGTCGAATGCTTCTCGGGACTCGGCAGGACCTGCCCTATACgagatcgggag                                                        >  2:849813/1‑291 (MQ=255)                                                                                                                                                                                                                             gcagcaGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCTGCGTCGAATGCTGCTCGGGACTCGGTAGGAACTGCCCTATACGGCCAAGCACCAGGt                                                    <  3:597809/301‑1 (MQ=255)                                                                                                                                                                                                                              cagcagCCCCGGCAGTGGAAGCCCAAGCGCAGAAAGAGGGGGGCGCTGCGCACTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGCT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCTGCGTCGAATGCTGCTCGGGACTCGGCAGGAACTGCCCTATACGGCCAAGCACCAGGTc                                                   <  2:626627/301‑1 (MQ=255)                                                                                                                                                                                                                                   gcCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCTGCGTCGAATGCTGCTCGGGACTCGGCAGGAACTGCCCTATACGGCCAAGCACCAGGTCGACAt                                              >  4:650494/1‑301 (MQ=255)                                                                                                                                                                                                                                     cccGGCAGTGGCATCTCAGGGGCAGAAAGAGGGTGGCGCTGCGCGTTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCTGCGTCGAATGCTGCTCGGGACTCGGCAGGAACTGCCCTATACGGCCAAGCACCAGGTCGACATTg                                            <  3:577940/301‑1 (MQ=255)                                                                                                                                                                                                                                                  gCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGTGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCTGCGTCGAATGCTGCTCGGGACTCGGCAGGAACTGCCCTATACGGCCAAGCACCAGGTCGACATTGGCCTTGTCGCCgg                               >  4:309230/1‑301 (MQ=255)                                                                                                                                                                                                                                                  gCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCTGCGTCGAATGCTGCCCGGGACTCGGCAGGAACTGCCCTATACGGCCAAGCACCAGGTCGACATTGGCCTTGTCGCCgg                               >  2:307912/1‑301 (MQ=255)                                                                                                                                                                                                                                                           cAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCTGCGTCGAATGCTGCTCGGGACTCGGCAGGAACTGCCCTATACGGCCAAGCACCAGGTCGACATTGGCCTTGTCGCCGGTCAGGAACa                      >  3:83196/1‑301 (MQ=255)                                                                                                                                                                                                                                                            aGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCTGCGTCGAATGCTGCTCGGGACTCGGCAGGAACTGCCCTATACGGCCAAGCACCAGGTCGACATTGGCCTTGTCGCCGGTCAGGAACAg                     <  1:83195/301‑1 (MQ=255)                                                                                                                                                                                                                                                                  agGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCTGCGTCGAATGCTGCTCGGGACTCGGCAGGAACTGCCCTATACGGCCAAGCACCAGGTCGACATTGGCCTTGTCGCCGGCCAGGAACCGCCAGtt               >  2:747693/1‑301 (MQ=255)                                                                                                                                                                                                                                                                   ctcttccgatctgcgcTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCTGCGTCGAATGCTGCTCGGGACTCGGCAGGAACTGCCCTATACGGCCAAGCACCAGGTCGACATTGGCCTTGTCGCCGGTCAGGAACAGCCAGTTc              <  2:603917/289‑1 (MQ=255)                                                                                                                                                                                                                                                                    gggAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCTGCGTCGAATGCTGCTCGGGACTCGGCAGGAACTGCCCTATACGGCCAAGCACCAGGTCGACATTGGCCTTGTCGCCGGTCAGGAACAGCCAGTTCg             <  3:95113/301‑1 (MQ=255)                                                                                                                                                                                                                                                                               gcgcTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCTGCGTCGAATGCTGCTCGGGACTCGGCAGGAACTGCCCTATACGGCCAAGCACCAGGTCGACATTGGCCTTGTCGCCGGTCAGGAACAGCCAGTTcagatcggaagag  >  4:604116/1‑289 (MQ=255)                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                      AGGGCAGCACGCTGGCGGGGACTGTGATATCGCTGGCGCCGACCCGGGCTTGCAATTGAGCGTCGCTACTGGAGACGCCCTCGCGGTAAAGGCGTTTGCCTGCTTGTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCTTATCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGAT‑ACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCTGCGTCGAATGCTGCTCGGGACTCGGCAGGAACTGCCCTATACGGCCAAGCACCAGGTCGACATTGGCCTTGTCGCCGGTCAGGAACAGCCAGTTCGGCCCATCCACC  >  AE015451/3950883‑3951453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑