Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451	1,932,640	+C	intergenic (‑107/+43)	rluA ← / ← minE	23S rRNA (746) and tRNA (32) pseudouridine synthase/Cell division topological specificity factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	1,932,638	1	.	C	100.0%	137.6 / NA	39	intergenic (‑105/+45)	rluA/minE	23S rRNA (746) and tRNA (32) pseudouridine synthase/Cell division topological specificity factor
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (23/16);  total (23/16)

GATGCCGGAGGTTTCCCAGTCCAGACGGTGCACGATCAGCGCATCGGGGTAGCCGTTTTCCTGCAGACGGGTGATCAGGCAGTCCTTGTTGTCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATGATCTGTAGACGCTCTTTCGCTACCGACGCGCTGGTCTGTTTCTGTCTGCCACGAAAGAAGTC  >  AE015451/1932345‑1932925                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                gatgCCGGAGGTTTCCCAGTCCAGACGGTGCACGATCAGCGCATCGGGGTAGCCGTTTTCCTGCAGACGGGTGATCAGGCAGTCCTTGTTGTCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑cccgc                                                                                                                                                                                                                                                                                            <  1:351323/299‑4 (MQ=255)                                aCGATCAGCGCATCGGGGTAGCCGTTTTCCTGCAGACGGGTGATCAGGCAGTCCTTGTTGTCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCATCCCCAAATTGTCGAc                                                                                                                                                                                                                                                             >  1:439984/1‑300 (MQ=255)                                   aTCAGCGCATCGGGGTAGCCGTTTTCCTGCAGACGGGTGATCAGGCAGTCCTTGTTGTCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTAc                                                                                                                                                                                                                                                          <  1:153225/300‑1 (MQ=255)                                        cgcATCGGGGTAGCCGTTTTCCTGCAGACGGGTGATCAGGCAGTCCTTGTTGTCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCgg                                                                                                                                                                                                                                                      >  1:698486/1‑299 (MQ=255)                                        cgcATCGGGGTAGCCGTTTTCCTGCAGACGGGTGATCAGCCAGTCCTTGTTGTCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGCTCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTAcg                                                                                                                                                                                                                                                         <  1:56511/296‑1 (MQ=255)                                                ggTAGCCGTTTTCCTGCAGACGGGTGATCAGGCAGTCCTTGTTGTCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACt                                                                                                                                                                                                                                                            >  1:652573/1‑285 (MQ=255)                                                                                          tgtCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagc                                                                                                                                                                                                            <  1:401620/291‑1 (MQ=255)                                                                                                                     gcagcagGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGTGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCg                                                                                                                                                                                                                                                               >  1:10601/1‑213 (MQ=255)                                                                                                                           gggTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGcc                                                                                                                                                                 <  1:390592/301‑1 (MQ=255)                                                                                                                               cGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGc                                                                                                                                                                                                                         <  2:652548/241‑1 (MQ=255)                                                                                                                                    tgttgATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTc                                                                                                                                                          >  1:521556/1‑299 (MQ=255)                                                                                                                                        gATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGc                                                                                                                                                                                        <  2:301744/265‑1 (MQ=255)                                                                                                                                        gATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGGTTTCCAGc                                                                                                                                                                                        >  1:301752/1‑265 (MQ=255)                                                                                                                                           cACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATAttt                                                                                                                                                  <  2:698461/300‑1 (MQ=255)                                                                                                                                                  aTGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGttt                                                                                                                                                                   >  1:439268/1‑275 (MQ=255)                                                                                                                                                          gTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCCCGATATTTACATCATCGTTGCCGATGTTCACATTTTTGCCGATCACTTCcagc                                                                                                                                  >  2:441587/1‑301 (MQ=255)                                                                                                                                                                         cTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCGGTTCTTTCTGc                                                                                                                      >  1:750962/1‑298 (MQ=255)                                                                                                                                                                           ggACATTCGAGATCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGttctttct                                                                                                                        <  1:220455/294‑1 (MQ=255)                                                                                                                                                                               aTTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGcgcg                                                                                                                >  1:602861/1‑298 (MQ=255)                                                                                                                                                                                                 aGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCCCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAGGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGt                                                                                                      <  1:601332/290‑1 (MQ=255)                                                                                                                                                                                                          gAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTg                                                                                                                                                                   <  2:439248/220‑1 (MQ=255)                                                                                                                                                                                                            aaaTAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAATGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATCTGCTCATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGttcttt                                                                                                                          >  2:383497/1‑259 (MQ=255)                                                                                                                                                                                                              aTAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCCCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCCGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACat                                                                                                                                                      >  1:190116/1‑229 (MQ=255)                                                                                                                                                                                                                                   gCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTTCGCCACGATGATCt                                                            >  1:756658/1‑298 (MQ=255)                                                                                                                                                                                                                                                     ggCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTcc                                                                                                                                     <  2:840216/207‑1 (MQ=255)                                                                                                                                                                                                                                                     ggCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTcc                                                                                                                                     >  1:840242/1‑207 (MQ=255)                                                                                                                                                                                                                                                        aaGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTCCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATGATCTGTAGACGCTCTTTCGCTACCg                                       >  1:973613/1‑298 (MQ=255)                                                                                                                                                                                                                                                          gCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGc                                                                                                             >  2:871125/1‑226 (MQ=255)                                                                                                                                                                                                                                                             cGCTCCTACAAAGGATATGCGTCCCCTGTAGTAGCGGGCTTGCCCGCGAAGGGCTGAAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGttcttt                                                                                                                          <  2:641336/210‑1 (MQ=255)                                                                                                                                                                                                                                                                     cAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTTGCCGCGCTCGTTCGCCACGATGCTCTGTAGACGCTCTTTCGCTACCGACCCGCTTGTCTGttt                       >  2:329148/1‑301 (MQ=255)                                                                                                                                                                                                                                                                         ggATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATGATCTGTAGACGCTCTTTCGCTACCGACGCGCTGGTCTGTTtctgt                   <  2:602837/301‑1 (MQ=255)                                                                                                                                                                                                                                                                             atGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCg                                                                                                   >  2:772564/1‑217 (MQ=255)                                                                                                                                                                                                                                                                             atGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCg                                                                                                   <  1:772590/217‑1 (MQ=255)                                                                                                                                                                                                                                                                             atGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTTCGCCACGATGATCTGTAGACGCTCTTTCGCTACCGACGCGCTGGTCTGTTtctgtctg                >  2:577957/1‑300 (MQ=255)                                                                                                                                                                                                                                                                               gCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGTGCTGCAATGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGt                                                                                                                                                                                                >  2:84793/1‑122 (MQ=255)                                                                                                                                                                                                                                                                                          aGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATGATCTGTAGACGCTCTTTCGCTACCGACGCGCTGGTCTGTTTCTGTCTGCCACGAAAGAAGt   >  1:923360/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                          aGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATGATCTGTAGACGCTCTTTCGCTACCGACCCGCTGGTCTGTTTCTGTCTGcc              >  2:439585/1‑289 (MQ=255)                                                                                                                                                                                                                                                                                           ggAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTTCGCCACGATGATCTGTAGACGCTCTTTCGCTACCGACGCGCTGGTCTGTTTCTGTCTGCCACGAAAGAAGTc  >  1:828533/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                           ggAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATGATCTGTAGACGctc                                                  <  2:439964/252‑1 (MQ=255)                                                                                                                                                                                                                                                                                           ggAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATGATCTGTAGACGCTCTTTCGCTACCGACGCGCTGGTCTGTTTCTGTCTGCCACGAAAGAAGTc  <  1:577979/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                GATGCCGGAGGTTTCCCAGTCCAGACGGTGCACGATCAGCGCATCGGGGTAGCCGTTTTCCTGCAGACGGGTGATCAGGCAGTCCTTGTTGTCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATGATCTGTAGACGCTCTTTCGCTACCGACGCGCTGGTCTGTTTCTGTCTGCCACGAAAGAAGTC  >  AE015451/1932345‑1932925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑