New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 2472115	22 (0.250)	29 (0.340)	29/538	3.8	45.9%	intergenic (+19/+130)	PP_2166/PP_2167	Anti‑anti‑sigma factor/putative PhoD‑like phosphatase
	?	AE015451	2472313 =	47 (0.560)					coding (1783/1851 nt)	PP_2167	putative PhoD‑like phosphatase
Rejected: Coverage evenness skew score above cutoff.

GACAGAAGGTGGGCCTGCTGCCGACGGTGGTGACTACCAATCCGGACATTTCACGGTTATTGCAGTCGATGGGCTTCGATCAGGTGTTCAACATCGTCGATCGACCGATACCCTGCACCGATTGCCTGACCGACTTGCCGTCGCAGGACCAGAATGAAGATGTGGTGCGCTCCAAGGTGCTGGAGGCGCACAAGATCCTCATGGGCCTGAATGACTCCAACCGCGAAGCCTTCCATGATCTGGTCAATGCGCTGGAGCGGAGCTGATCTATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCCTGCGCCCAAACACCAATGTGTGTCCACCCCCAATCAGGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCTGTAAAAACTCTGAATCTCACTACGCCCGGTTTTTAAACTCTGTAGCCTCACCCCCGCCAGCATCCAGCTGGTACACCCGCGCCGGCCGCCCCTGTTCATCAAAAAACACCTGTCCCAGCCCGGCACCATTCCACAGCCGTTCCCCGGCCTTGCTGTG  >  AE015451/2471831‑2472382                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                            gACAGAAGGTGGGCCTGCTGCCGACGGTGGTGACTACCAATCCGGACATTTCACGGTTATTGCAGTCGATGGGCTTCGATCAGGTGTTCAACATCGTCGATCGACCGATACCCTGCACCGATTGCCTGACCGACTTGCCGTCGCAGGACCAGAATGAAGATGTGGTGCGCTCCAAGGTGCTGGAGGCGCACAAGATCCTCATGGGCCTGAATGACTCCAACCGCGAAGCCTTCCATGATCTGGTCAATGCGCTGGAGCGGAGCTGATCTATAGCCTGTGCCGGCCTCTTCGCGGGTAAAc                                                                                                                                                                                                                                                              >  2:294929/1‑300 (MQ=255)     gAAGGTGGGCCTGCTGCCGACGGTGGTGACTACCAATCCGGACATTTCACGGTTATTGCAGTCGATGGGCTTCGATCAGGTGTTCAACATCGTCGATCGACCGATACCCTGCACCGATTGCCTGACCGACTTGCCGTCGCAGGACCAGAATGAAGATGTGGTGCGCTCCAAGGTGCTGGAGGCGCACAAGATCCTCATGGGCCTGAATGACTCCAACCGCGAAGCCTTCCATGATCTGGTCAATGCGCTGGAGCGGAGCTGATCTATAGCCTGTGCCGGCCTCTTCGCGGGTAAAccc                                                                                                                                                                                                                                                            <  1:294936/298‑1 (MQ=255)     gAAGGTGGGCCTGCTGCCGACGGTGGTGACTACCAATCCGGACATTTCACGGTTATTGCAGTCGATGGGCTTCGATCAGGTGTTCAACATCGTCGATCGACCGATACCCTGCACCGATTGCCTGACCGACTTGCCGTCGCAGGACCAGAATGAAGATGTGGTGCGCTCCAAGGTGCTGGAGGCGCACAAGATCCTCATGGGCCTGAATGACTCCAACCGCGAAGCCTTCCATGATCTGGTCAATGCGCTGGAGCGGAGCTGATCTATAGCCTGTGCCGGCCTCTTCGCGGGTAAAccc                                                                                                                                                                                                                                                            <  2:740012/298‑1 (MQ=255)         gTGGGCCTGCTGCCGACGGTGGTGACTACCAATCCGGACATTTCACGGTTATTGCAGTCGATGGGCTTCGATCAGGTGTTCAACATCGTCGATCGACCGATACCCTGCACCGATTGCCTGACCGACTTGCCGTCGCAGGACCAGAATGAAGATGTGGTGCGCTCCAAGGTGCTGGAGGCGCACAAGATCCTCATGGGCCTGAATGACTCCAACCGCGAAGCCTTCCATGATCTGGTCAATGCGCTGGAGCGGATCTGATCTATAGCCTGTGCCGGCCTCTTCGCGGGGAAACCCGCTCCt                                                                                                                                                                                                                                                      >  1:888469/1‑300 (MQ=255)          tGGGCCTGCTGCCGACGGTGGTGACTACCAATCCGGACATTTCACGGTTATTGCAGTCGATGGGCTTCGATCAGGTGTTCAACATCGTCGATCGACCGATACCCTGCACCGATTGCCTGACCGACTTGCCGTCGCAGGACCAGAATGAAGATGTGGTGCGCTCCAAGGTGCTGGAGGCGCACAAGATCCTCATGGGCCTGAATGACTCCAACCGCGAAGCCTTCCATGATCTGGTCAATGCGCTGGAGCGGAGCTGATCTATAGCCTGTGCCGGccgcccctgttcatcaa                                                                                                                                                                                                                                                              >  1:549586/1‑276 (MQ=255)          tGGGCCTGCTGCCGACGGTGGTGACTACCAATCCGGACATTTCACGGTTATTGCAGTCGATGGGCTTCGATCAGGTGTTCAACATCGTCGATCGACCGATACCCTGCACCGATTGCCTGACCGACTTGCCGTCGCAGGACCAGAATGAAGATGTGGTGCGCTCCAAGGTGCTGGAGGCGCACAAGATCCTCATGGGCCTGAATGACTCCAACCGCGAAGCCTTCCATGATCTGGTCAATGCGCTGGAGCGGAGCTGATCTATAGCCTGTGCCGGccgcccctgttcatcaa                                                                                                                                                                                                                                                              <  2:549565/291‑16 (MQ=255)                                           cGGACATTTCACGGTTATTGCAGTCGATGGGCTTCGATCAGGTGTTCAACATCGTCGATCGACCGATACCCTGCACCGATTGCCTGACCGACTTGCCGTCGCAGGACCAGAATGAAGATGTGGTGCGCTCCAAGGTGCTGGAGGCGCACAAGATCCTCATGGGCCTGAATGACTCCAACCGCGAAGCCTTCCATGATCTGGTCAATGCGCTGGAGCGGAGCTGATCTATAGCCTGTGCCGGccgcccctgttca                                                                                                                                                                                                                                                                  >  1:130384/1‑243 (MQ=255)                                           cGGACATTTCACGGTTATTGCAGTCGATGGGCTTCGATCAGGTGTTCAACATCGTCGATCGACCGATACCCTGCACCGATTGCCTGACCGACTTGCCGTCGCAGGACCAGAATGAAGATGTGGTGCGCTCCAAGGTGCTGGAGGCGCACAAGATCCTCATGGGCCTGAATGACTCCAACCGCGAAGCCTTCCATGATCTGGTCAATGCGCTGGAGCGGAGCTGATCTATAGCCTGTGCCGGccgcccctgttca                                                                                                                                                                                                                                                                  <  2:130381/254‑12 (MQ=255)                                                       ggTTATTGCAGTCGATGGGCTTCGATCAGGTGTTCAACATCGTCGATCGACCGATACCCTGCACCGATTGCCTGACCGACTTGCCGTCGCAGGACCAGAATGAAGATGTGGTGCGCTCCAAGGTGCTGGAGGCGCACAAGATCCTCATGGGCCTGAATGACTCCAACCGCGAAGCCTTCCATGATCTGGTCAATGCGCTGGAGCGGAGCTGATCTATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCCTGCGCCCAAACACCaa                                                                                                                                                                                                                             <  1:65779/279‑1 (MQ=255)                                                       ggTTATTGCAGTCGATGGGCTTCGATCAGGTGTTCAACATCGTCGATCGACCGATACCCTGCACCGATTGCCTGACCGACTTGCCGTCGCAGGACCAGAATGAAGATGTGGTGCGCTCCAAGGTGCTGGAGGCGCACAAGATCCTCATGGGCCTGAATGACTCCAACCGCGAAGCCTTCCATGATCTGGTCAATGCGCTGGAGCGGAGCTGATCTATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCCTGCGCCCAAACACCaa                                                                                                                                                                                                                             >  2:65777/1‑279 (MQ=255)                                                        gTTATTGCAGTCGATGGGCTTCGATCAGGTGTTCAACATCGTCGATCGACCGATACCCTGCACCGATTGCCTGACCGACTTGCCGTCGCAGGACCAGAATGAAGATGTGGTGCGCTCCAAGGTGCTGGAGGCGCACAAGATCCTCATGGGCCTGAATGACTCCAACCGCGAAGCCTTCCATGATCTGGTCAATGCGCTGGAGCGGAGCTGATCTATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCCTGCGCCCAAACACCAATGTGTGTCCACCCCCAATCAgg                                                                                                                                                                                                       <  2:454007/300‑1 (MQ=255)                                                              gCAGTCGATGGGCTTCGATCAGGTGTTCAACATCGTCGATCGACCGATACCCTGCACCGATTGCCTGACCGACTTGCCGTCGCAGGACCAGAATGAAGATGTGGTGCGCTCCAAGGTGCTGGAGGCGCACAAGATCCTCATGGGCCTGAATGACTCCAACCGCGAAGCCTTCCATGATCTGGTCAATGCGCTGGAGCGGAGCTGATCTATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCCTGCGCCCAAACACCAATGTGTGTCCACCCCCAATCAGGTGTAg                                                                                                                                                                                                  <  2:269192/299‑1 (MQ=255)                                                                                   ggTGTTCAACATCGTCGATCGACCGATACCCGGCACCGATTGCCTGACCGACTTGCCGTCGCAGGACCAGAATGAAGATGTGGTGCGCTCCAAGGTGCTGGAGGCGCACAAGATCCTCATGGGCCTGAATGACTCCAACCGCGAAGCCTTCCATGATCTGGTCAATGCGCTGGAGCGGAGCTGATCTATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCCTGCGCCCAAACACCAATGTGTGTCCACCCCCAATCAGGTGTAGGAGCGGGTTTACCCGCGAagag                                                                                                                                                                            <  2:888443/300‑1 (MQ=255)                                                                                      gtTCAACATCGTCGATCGACCGATACCCTGCACCGATTGCCTGACCGACTTGCCGTCGCAGGACCAGAATGAAGATGTGGTGCGCTCCAAGGTGCTGGAGGCGCACAAGATCCTCATGGGCCTGAATGACTCCAACCGCGAAGCCTTCCATGATCTGGTCAATGCGCTGGAGCGGAGCTGATCTATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCCTGCGCCCAAACACCAATGTGTGTCCAcccc                                                                                                                                                                                                               <  2:142059/262‑1 (MQ=255)                                                                                      gtTCAACATCGTCGATCGACCGATACCCTGCACCGATTGCCTGACCGACTTGCCGTCGCAGGACCAGAATGAAGATGTGGTGCGCCCCAAGGTGCTGGAGGCGCACAAGATCCTCATGGGCCTGAATGACTCCAACCGCGAAGCCTTCCATGATCTGGTCAATGCGCTGGAGCGGAGCTGATCTATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCCTGCGCCCAAACACCAATGTGTGTCCAcccc                                                                                                                                                                                                               >  1:142062/1‑262 (MQ=255)                                                                                                        aCCGATACCCTGCACCGATTGCCTGACCGACTTGCCGTCGCAGGACCAGAATGAAGATGTGGTGCGCTCCAAGGTGCTGGAGGCGCACAAGATCCTCATGGGCCTGAATGACTCCAACCGCGAAGCCTTCCATGATCTGGTCAATGCGCTGGAGCGGAGCTGATCTATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCCTGCGCCCAAACACCAATGTGTGTCCACCCCCAATCAgg                                                                                                                                                                                                       <  2:666726/252‑1 (MQ=255)                                                                                                           gATACCCTGCACCGATTGCCTGACCGACTTGCCGTCGCAGGACCAGAATGAAGATGTGGTGCGCTCCAAGGTGCTGGAGGCGCACAAGATCCTCATGGGCCTGAATGACTCCAACCGCGAAGCCTTCCATGATCTGGTCAATGCGCTGGAGCGGAGCTGATCTATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCCTgc                                                                                                                                                                                                                                          >  2:5036/1‑214 (MQ=255)                                                                                                           gATACCCTGCACCGATTGCCTGACCGACTTGCCGTCGCAGGACCAGAATGAAGATGTGGTGCGCTCCAAGGTGCTGGAGGCGCACAAGATCCTCATGGGCCTGAATGACTCCAACCGCGAAGCCTTCCATGATCTGGTCAATGCGCTGGAGCGGAGCTGATCTATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCCTgc                                                                                                                                                                                                                                          <  1:5038/214‑1 (MQ=255)                                                                                                                    cACCGATTGCCTGACCGACTTGCCGTCGCAGGACCAGAATGAAGATGTGGTGCGATCCAAGGTGCTGGAGGCGCACAAGATCCTCATGGGCCTGAATGACTCCAACCGCGAAGCCTTCCATGATCTGGTCAATGCGCTGGAGCGGAGCTGATCTATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCCTGCGCCCAAACACCAATGTGTGTCCACCCCCAATCAGGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCTGTAAAAACTCTGAATCTCAc                                                                                                                                           <  1:95636/300‑1 (MQ=255)                                                                                                                                                        gAATGAAGATGTGGTGCGCGCCAAGGTGCTGGAGGCGCACAAGATCCTCATGGGCCTGAATGACTCCAACCGCGAAGCCTTCCATGATCTGGTCAATGCGCTGGAGCGGGGCTGATCTATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCCTGCGCCCAAACACCAATGTGTGTCCACCCCCAATCAGGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCTGTAAAAACTCTGAATCTCACTACGCCCGGTTTTTAAACTCTGTAGcc                                                                                                                <  2:53886/291‑1 (MQ=255)                                                                                                                                                                    ggtgTGCTCCAAGGTGCTGGAGGCGCACAAGATCCTCATGGGCCTGAATGACTCCAACCGCGAAGCCTTCCATGATCTGGTCAATGCGCTGGAGCGGAGCTGATCTATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCCTGCGCCCAAACACCAATGTGTGTCCACCCCCAATCAGGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCTGTAAAAACTCTGAATCTCACTACGCCCGGTTTTTAAACTCTGTAGCCTCACCCCCGCCAGCATCCAg                                                                                            <  2:133200/299‑1 (MQ=255)                                                                                                                                                                        cgcTCCAAGGTGCTGGAGGCGCACAAGATCCTCATGGGCCTGAATGACTCCAACCGCGAAGCCTTCCATGATCTGGTCAATGCGCTGGAGCGGAGCTGATCTATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCCTGCGCCCAAACACCAATGTGTGTCCACCCCCAATCAGGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCTGTAAAAACTCTGAATCTCACTACGCCCGGTTTTTAAACTCTGTAGCCTCACCCCCGCCAGCATCCAGCTGGt                                                                                       >  1:892204/1‑300 (MQ=255)                                                                                                                                                                                                       tCATGGGCCTGAATGACTCCAACCGCGAAGCCTTCCATGATCTGGTCAATGCGCTGGAGCGGAGCTGATCTATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCCTGCGCCCAAACACCAATGTGTGTCCACCCCCAATCAGGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCTGTAAAAACTCTGAATCTCACTACGCCCGGTTTTTAAACTCTGTAGCCTCACCCCCGCCAGCATCCAGCTGGTACACCCGCGCCGGCCGCCCCTGt                                                                >  2:571991/1‑292 (MQ=255)                                                                                                                                                                                                                                   aGCCTTCCATGATCTGGTCAATGCGCTGGAGCGGAGCTGATCTATAGCCTGTGCCGGCCTCTTCTCGGGTAAACCCGCTCCTACGACGGCCTGCGCCCAAACACCAATGTGTGTCCACCCCCAATCAGGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCTGTAAAAACTCTGAATCTCACTACGCCCGGTTTTTAAACTCTGTAGCCTCACCCCCGCCAGCATCCAGCTGGTACACCCGCGCCGGCCGCCCCTGTTCATCAAAAAACACCTGTCCCAGCCCGGCACCATTc                            <  1:879071/300‑1 (MQ=255)                                                                                                                                                                                                                                                                   ggagcTGATCTATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCCTGCGCCCAAACACCAATGTGTGTCCACCCCCAATCAGGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCTGTAAAAACTCTGAATCTCACTACGCCCGGTTTTTAAACTCTGTAGCCTCACCCCCGCCAGCATCCAGCTGGTACACCCGCGCCGGCCGCCCCTGTTCATCAAAAAACACCTGTCCCAGCCCGGCACCATTCCACAGCCGTTCCCCGGCCTTGCtgtg  >  2:577597/1‑294 (MQ=255)                                                                                                                                                                                                                                                                                     gtgCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCCTGCGCCCAAACACCAATGTGTGTCCACCCCCCATCAGGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCTGTAAAAACTCTGAATCTCACTACGCCCGGTTTTTAAACTCTGTAGCCTCACCCCCGCCAGCATCCAGCTGGTACACCCGCGCCGGCCGCCCCTGTTCATCAAAAAACACCTGTCCCAGCCCGGCAcc                                <  2:892178/246‑1 (MQ=255)                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                            GACAGAAGGTGGGCCTGCTGCCGACGGTGGTGACTACCAATCCGGACATTTCACGGTTATTGCAGTCGATGGGCTTCGATCAGGTGTTCAACATCGTCGATCGACCGATACCCTGCACCGATTGCCTGACCGACTTGCCGTCGCAGGACCAGAATGAAGATGTGGTGCGCTCCAAGGTGCTGGAGGCGCACAAGATCCTCATGGGCCTGAATGACTCCAACCGCGAAGCCTTCCATGATCTGGTCAATGCGCTGGAGCGGAGCTGATCTATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGACGGCCTGCGCCCAAACACCAATGTGTGTCCACCCCCAATCAGGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCTGTAAAAACTCTGAATCTCACTACGCCCGGTTTTTAAACTCTGTAGCCTCACCCCCGCCAGCATCCAGCTGGTACACCCGCGCCGGCCGCCCCTGTTCATCAAAAAACACCTGTCCCAGCCCGGCACCATTCCACAGCCGTTCCCCGGCCTTGCTGTG  >  AE015451/2471831‑2472382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.