Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	AE015451	5,681,415	+CGGG	intergenic (‑133/+71)	PP_4986 ← / ← PP_4987	Channel protein, hemolysin III family/putative Chemotaxis protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 5681415	0 (0.000)	60 (0.700) +CGGG	57/544	0.9	100%	intergenic (‑133/+71)	PP_4986/PP_4987	Channel protein, hemolysin III family/putative Chemotaxis protein
	?	AE015451	5681416 =	0 (0.000)					intergenic (‑134/+70)	PP_4986/PP_4987	Channel protein, hemolysin III family/putative Chemotaxis protein

TACAGCAACAGCAGCGTGCCGCCGTAGATGGAGAAGCTGACGATCTTCCACGGGTCGCCTTGCAGGCCCGCGGCCACGATCAGCCAGATGGCACCAATAGCAGCGAGGACGGCACCGACCAGGTGGGTCCAGGCGTTGAAGCGTTCACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCTGCCCAGCGCCACATCCACGTAGTTCAGCTGGCTGTCGAGCTTGCACGAGCGCGGAATGTCCTGCAGCAACAGCGCCAGGTAGCGCAAACCGGTATCGCCCAACGCATTGAGCACCACCA  >  AE015451/5681125‑5681715                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                            gcaacagcagcgtgccgccgtagatggagaagctgacgatcttccacgggtcgccttgcaggcccgcggccacgatcagccagatggcaccaatagcagcgaggacggcaccgaccaggtgggtccaggcgttgaagcgttcaccgtaatacatgcaaacacagacctcctgaggtgggctgggttcctgttgtgatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCtgctgc                                                                                                                                                                                                                                                                                                      >  1:2471‑M2/292‑299 (MQ=255)     cagcagcgtgccgccgtagatggagaagctgacgatcttccacgggtcgccttgcaggcccgcggccacgatcagccagatggcaccaatagcagcgaggacggcaccgaccaggtgggtccaggcgttgaagcgttcaccgtaatacatgcaaacacagacctcctgaggtgggctgggttcctgttgtgatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGcc                                                                                                                                                                                                                                                                                                >  1:576368‑M2/288‑301 (MQ=255)                cgccgtagatggagaagctgacgatcttccacgggtcgccttgcaggcccgcggccacgatcagccagatggcaccaatagcagcgaggacggcaccgaccaggtgggtccaggcgttgaagcgttcaccgtaatacatgcaaacacagacctcctgaggtgggctgggttcctgttgtgatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATc                                                                                                                                                                                                                                                                                           <  2:338256‑M2/19‑1 (MQ=255)                              aagctgacgatcttccacggttcgccttgcaggcccgcggccacgatcagccagatggcaccaatagcagcgaggacggcaccgaccaggtgggtccaggcgttgaagcgttcaccgtaatacatgcaaacacagacctcctgaggtgggctgggttcctgttgtgatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTccc                                                                                                                                                                                                                                                                        <  2:2471‑M2/38‑1 (MQ=255)                                gctgacgatcttccacgggtcgccttgcaggcccgcggccacgatcagccagatggcaccaatagcagcgaggacggcaccgaccaggtgggtccaggcgttgaagcgttcaccgtaatacatgcaaacacagacctcctgaggtgggctgggttcctgttgtgatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccggggc                                                                                                                                                                                                                                                                                                            >  2:103285‑M2/261‑262 (MQ=255)                                gctgacgatcttccacgggtcgccttgcaggcccgcggccacgatcagccagatggcaccaatagcagcgaggacggcaccgaccaggtgggtccaggcgttgaagcgttcaccgtaatacatgcaaacacagacctcctgaggtgggctgggttcctgttgtgatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGac                                                                                                                                                                                                                                                                                      <  1:103287‑M2/24‑1 (MQ=255)                                   gacgatcttccacgggtcgccttgcaggcccgcggccacgatcagccagatggcaccaatagcagcgaggacggcaccgaccaggtgggtccaggcgttgaagcgttcaccgtaatacatgcaaacacagacctcctgaggtgggctgggttcctgttgtgatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACt                                                                                                                                                                                                                                                                     <  1:251047‑M2/41‑1 (MQ=255)                                         cttccacgggtcgccttgcaggcccgcggccacgatcagccagatggcaccaatagcagcgaggacggcaccgaccaggtgggtccaggcgttgaagcgttcaccgtaatacatgcaaacacagacctcctgaggtgggctgggttcctgttgtgatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCATCCCTATCGCCacac                                                                                                                                                                                                                                                                                    >  2:544267‑M2/252‑277 (MQ=255)                                           tccacgggtcgccttgcaggcccgcggccacgatcagccagatggcaccaatagcagcgaggacggcaccgaccaggtgggtccaggcgttgaagcgttcaccgtaatacatgcaaacacagacctcctgaggtgggctgggttcctgttgtgatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGacc                                                                                                                                                                                                                                                              >  1:568292‑M2/250‑297 (MQ=255)                                                gggtcgccttgcaggcccgcggcaacgatcagccagatggcaccaatagcagcgaggacggcaccgaccaggtgggtccaggcgttgaagcgttcaccgtaatacatgcaaacacagacctcctgaggtgggctgggttcctgttgtgatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTAt                                                                                                                                                                                                                                                                                            <  2:576346‑M2/18‑1 (MQ=255)                                                       cttgcaggcccgcggccacgatcagccagatggcaccaatagcagcgaggacggcaccgaccaggtgggtccaggcgttgaagcgttcaccgtaatacatgcaaacacagacctcctgaggtgggctgggttcctgttgtgatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGcc                                                                                                                                                                                                                                                      >  1:140251‑M2/238‑293 (MQ=255)                                                               cccgcggccacgatcagccagatggcaccaatagcagcgaggacggcaccgaccaggtgggtccaggcgttgaagcgttcaccgtaatacatgcaaacacagacctcctgaggtgggctgggttcctgttgtgatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCCCTCCAGACCCCCAGGCCCTGCGCCTCACATcc                                                                                                                                                                                                                                       >  1:711833‑M2/230‑300 (MQ=255)                                                                             cagccagatggcaccaatcgcagcgaggacggcaccgaccaggtgggtccaggcgttgaagcgttcaccgtaatacatgcaaacacagacctcctgaggtgggctgggttcctgttgtgatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAAcc                                                                                                                                                                                                                                  <  1:879046‑M2/76‑1 (MQ=255)                                                                                ccagatggcaccaatagcagcgaggacggcaccgaccaggtgggtccaggcgttgaagcgttcaccgtaatacatgcaaacacagacctcctgaggtgggctgggttcctgttgtgatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcctgaaccgccgggGCTGCTGCGCAGCCCTATcgc                                                                                                                                                                                                                                                                                         >  2:172704‑M2/213‑233 (MQ=255)                                                                                  agatggcaccaatagcagcgaggacggcaccgaccaggtgggtccaggcgttgaagcgttcaccgtaatacatgcaaacacagacctcctgaggtgggctgggttcctgttgtgatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTc                                                                                                                                                                                                                                             >  2:238517‑M2/211‑275 (MQ=255)                                                                                  agatggcaccaatagcagcgaggacggcaccgaccaggtgggtccaggcgttgaagcgttcaccgtaatacatgcaaacacagacctcctgaggtgggctgggttcctgttgtgatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCCCATCCTAACCAAGGTc                                                                                                                                                                                                                            >  2:56373‑M2/211‑292 (MQ=255)                                                                                  agatggcaccaatagcagcgaggacggcaccgaccaggtgggtccaggcgttgaagcgttcaccgtaatacatgcaaacacagacctcctgaggtgggctgggttcctgttgtgatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATcc                                                                                                                                                                                                                                       <  1:238523‑M2/71‑1 (MQ=255)                                                                                      ggcaccaatagcagcgaggacggcaccgaccaggtgggtccaggcgttgaagcgttcaccgtaatacatgcaaacacagacctcctgaggtgggctgggttcctgttgtgatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTgc                                                                                                                                                                                                                                                  <  2:140248‑M2/60‑1 (MQ=255)                                                                                            aatagcagcgaggacggcaccgaccaggtgggtccaggcgttgaagcgttcaccgtaatacatgcaaacacagacctcctgaggtgggctgggttcctgttgtgatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACAt                                                                                                                                                                                                                                         <  2:570167‑M2/69‑1 (MQ=255)                                                                                              tagcagcgaggacggcaccgaccaggtgggtccaggcgttgaagcgttcaccgtaatacatgcaaacacagacctcctgaggtgggctgggttcctgttgtgatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCt                                                                                                                                                                                                                                                    >  2:428586‑M2/199‑256 (MQ=255)                                                                                                                 gaccaggtgggtccaggcgttgaagcgttcaccgtaatacatgcaaacacagacctcctgaggtgggctgggttcctgttgggatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAgg                                                                                                                                                                                                                         <  2:358745‑M2/85‑1 (MQ=255)                                                                                                                    caggtgggtccaggcgttgaagcgttcaccgtaatacatgcaaacacagacctcctgaggtgggctgggttcctgttgtgatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCCCATCCTAACCAAGGTCCGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATg                                                                                                                                                                                      >  1:595411‑M2/177‑296 (MQ=255)                                                                                                                       gtgggtccaggcgttgaagcgttcaccgtaatacatgcaaacacagacctcctgaggtgggctgggttcctgttgtgatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGAc                                                                                                                                                                                  <  1:180009‑M2/124‑1 (MQ=255)                                                                                                                                      gaagcgttcaccgtaatacatgcaaacacagacctcctgaggtgggctgggttcctgttgtgatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAg                                                                                                                                                                                                                               <  1:172708‑M2/79‑1 (MQ=255)                                                                                                                                                 cttagtacatccaaacacagacctcctgaggtgggctgggttcctgttgtgatgctgatggcttcgttctttgtgggagcagccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCa                                                                                                                                                                                                                    <  2:349611‑M2/90‑1 (MQ=255)                                                                                                                                                 cgtaatacatgcaaacacagacctcctgaggtgggctgggttcctgttgtgatgctgatggcttcgttctttgtgggagcggccctgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGTTCCCACTCCAGACCACCAGGCCCTGCGCTCCACATCCTAACCAAGGTCAGGTTGCa                                                                                                                                                                                                                    >  1:349622‑M2/148‑237 (MQ=255)                                                                                                                                                   taatacatgcaaacacagacctcctgaggtgggctgggttcctgttgtgatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTAccc                                                                                                                                                                    >  2:166324‑M2/146‑283 (MQ=255)                                                                                                                                                   taatacatgcaaacacagacctcctgaggtgggctgggttcctgttgtgatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTg                                                                                                                                                          <  1:166328‑M2/148‑1 (MQ=255)                                                                                                                                                                          ctgaggtgggctgggttcctgttgtgatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCa                                                                                                                             >  1:572956‑M2/123‑299 (MQ=255)                                                                                                                                                                              ggtgggctgggttccttttgtgatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCTg                                                                                                                        <  1:544288‑M2/182‑1 (MQ=255)                                                                                                                                                                                      gggttcctgttgtgatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCTGCCCAgc                                                                                                                  <  1:56375‑M2/188‑1 (MQ=255)                                                                                                                                                                                        gttcctgttgtgatgctgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCTGc                                                                                                                       <  2:568271‑M2/183‑1 (MQ=255)                                                                                                                                                                                                        tgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCTGCCCAGCGCCACAt                                                                                                           <  1:527002‑M2/195‑1 (MQ=255)                                                                                                                                                                                                        tgatggcttcgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCCCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCt                                                                                                                         >  2:526981‑M2/93‑273 (MQ=255)                                                                                                                                                                                                                 cgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCt                                                                                                                                               >  1:197842‑M2/84‑242 (MQ=255)                                                                                                                                                                                                                 cgttctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCt                                                                                                                                               <  2:197838‑M2/159‑1 (MQ=255)                                                                                                                                                                                                                    tctttgtgggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCCGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCCCACCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACCCACCAGCCTTTCCAATGCGAc                                                                                                                                                                                  >  2:861497‑M2/81‑204 (MQ=255)                                                                                                                                                                                                                       ttgtgggagcgaccttgtgtcgcgaaaggggcacgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCAACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATc                                                                                                                                                                                                                <  2:680821‑M2/94‑1 (MQ=255)                                                                                                                                                                                                                           gggagcggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTACGGTACCCGCCCCACCTGCTCACCCACCTGCAACGCTGCCAGCCCCAACCTGCCCAGCGCCACATCCACGGAGTTCAGCTGGCTGTCGAGCTTGc                                                                             >  1:460906‑M2/74‑298 (MQ=255)                                                                                                                                                                                                                                cggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCTGCCCAGCGCCACATCCACGTAGTTCAGCTGGCTGTCg                                                                                    <  1:927165‑M2/218‑1 (MQ=255)                                                                                                                                                                                                                                cggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCTGCCCAGCGCCACATCCACGTAGTTCAGCTGGCTGTCGAGCTTGCACGAgcg                                                                      <  1:428606‑M2/232‑1 (MQ=255)                                                                                                                                                                                                                                 ggccttgtgtcgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCTGCCCAGCGCCACATCCACGTAGTTCAGCTGGCTGTCGAGCTTGCAc                                                                           >  2:140270‑M2/68‑294 (MQ=255)                                                                                                                                                                                                                                           cgcgaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCTGCCCAGCGCCACATCCACGTAGTTCAGCTGGCTGTCGAGCTTGCACGAGCGCGGAATg                                                               <  1:556708‑M2/239‑1 (MQ=255)                                                                                                                                                                                                                                              gaaaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCTGCCCAGCGCCACATCCACGTAGTTCAGCTGGCTGTCGAGCTTGCACGAGCGCGGAATGTCCTg                                                          <  1:140273‑M2/244‑1 (MQ=255)                                                                                                                                                                                                                                                aaggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCTGCCCAGCGCCACATCCACGTAGTTCAGCTGGCTGTCGAGCTTGCACGAGCGCGGAATGTCCTgcagc                                                      >  1:728381‑M2/53‑300 (MQ=255)                                                                                                                                                                                                                                                  ggggcgcgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCTGCCCAGCGCCACATCCACGTAGTTCAGCTGGCTGTCGAGCTTGCACGAGCGCGGAATGTCCTg                                                          >  2:676754‑M2/51‑294 (MQ=255)                                                                                                                                                                                                                                                        cgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCTCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCTGCCCAGCGCCACATCCACGTAGTTCAGCTGGCTGTCGAGCTTGCACGAGCGCGGAATGTCCTGCAGCAACCGCGcc                                             >  2:199144‑M2/45‑301 (MQ=255)                                                                                                                                                                                                                                                        cgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCTGCCCAGCGCCACATCCACGTAGTTCAGCTGGCTGTc                                                                                     <  2:568826‑M2/217‑1 (MQ=255)                                                                                                                                                                                                                                                        cgaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCTGCCCAGCGCCACATCCACGTAGTTCAGCTGGCTGTc                                                                                     >  1:568847‑M2/45‑261 (MQ=255)                                                                                                                                                                                                                                                         gaagcggccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCCCCAACCTGCCCAGCGCCACATCCACGTAGTTCAGCTGGCTGTCGAGCTTGCACGAGCGCGGAATGTCCTgcagc                                                      >  2:628055‑M2/44‑291 (MQ=255)                                                                                                                                                                                                                                                             cggccccatgctttcattgcaactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCTGCCCAGCGCCACATCCACGTAGTTCAGCTGGCTGTCGAGCTTGCACGAGCGCGGAATGTCCTGCAGCAACAGCGCCAGGt                                         <  2:728355‑M2/261‑1 (MQ=255)                                                                                                                                                                                                                                                                ccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCTGCCCAGCGCCACATCCACGTAGTTCAGCTGGCTGTCGAGCTTGCACg                                                                          >  1:50716‑M2/37‑264 (MQ=255)                                                                                                                                                                                                                                                                ccccaggctttcattgccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCTGCCCAGCGCCACATCCACGTAGTTCAGCTGGCTGTCGAGCTTGCACGAGCGCGGAATGTCCTgcagc                                                      >  2:804747‑M2/37‑284 (MQ=255)                                                                                                                                                                                                                                                                                ccactgcatgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCTGCCCAGCGCCACATCCACGTAGTTCAGCTGGCTGTCGAGCTTGCACGAGCGCGGAATGTCCTGCAGCAACAGCGCCAGGTAGCGCAAACCGGTATCGcc                      <  1:676779‑M2/280‑1 (MQ=255)                                                                                                                                                                                                                                                                                       atgaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCTGCCCAGCGCCACATCCACGTAGTTCAGCTGGCTGTCGAGCTTGCACGAGCGCGGAATGTCCTGCAGCAACAGCGCCAGGTAGCGCAAACCGGTATCGCCCAACGc                >  2:236369‑M2/14‑299 (MQ=255)                                                                                                                                                                                                                                                                                         gaaccgccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCTGCCCAGCGCCACATCCACGTAGTTCAGCTGGCTGTCGAGCTTGCACGAGCGCGGAATGTCCTGCAGCAACAGCGCCAGGTAGCGCAAACCGGTAt                          <  2:572934‑M2/276‑1 (MQ=255)                                                                                                                                                                                                                                                                                              gccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCTGCCCAGCGCCACATCCACGTAGTTCAGCTGGCTGTCGAGCTTGCACGAGCGCGGAAt                                                                <  2:460885‑M2/238‑1 (MQ=255)                                                                                                                                                                                                                                                                                              gccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCTGCCCAGCGCCACATCCACGTAGTTCAGCTGGCTGTCGAGCTTGCACGAGCGCGGAATGTCCTGCAGCAACAGCGCCAGGTAGCGCAAACCGGTATCGCCCAACGCATTg            >  2:738087‑M2/7‑296 (MQ=255)                                                                                                                                                                                                                                                                                               ccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCTGCCCAGCGCCACATCCACGTAGTTCAGCTGGCTGTCGAGCTTGCACGAGCGCGGAATGTCCTGCAGCAACAGCGCCAGGTAGCGCAAACCGGTATCGc                       >  2:395944‑M2/6‑284 (MQ=255)                                                                                                                                                                                                                                                                                               ccgggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCTGCCCAGCGCCACATCCACGTAGTTCAGCTGGCTGTCGAGCTTGCACGAGCGCGGAATGTCCTGCAGCAACAGCGCCAGGTAGCGCAAACCGGTATCGc                       <  1:395956‑M2/279‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 gggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCTGCCCAGCGCCACATCCACGTAGTTCAGCTGGCTGTCGAGCTTGCACGAGCGCGGAATGTCCTGCAGCAACAGCGCCAGGTAGCGCAAACCGGTATCGCCCAAc                  <  1:694172‑M2/284‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 gggGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCTGCCCAGCGCCACATCCACGCAGCTCAGCTGGCTGTCGAGCt                                                                                >  2:694147‑M2/4‑225 (MQ=255)                                                                                                                                                                                                                                                                                                    gctgctGCGCAGCCCTATCGGGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCTGCCCAGCGCCACATCCACGTAGTTCAGCTGGCTGTCGAGCTTGCACGAGCGCGGAATGTCCTGCAGCAACAGCGCCAGGTAGCGCAAACCGGTATCGCCCAACGCATTGAGcaccacca  <  1:236374/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                            TACAGCAACAGCAGCGTGCCGCCGTAGATGGAGAAGCTGACGATCTTCCACGGGTCGCCTTGCAGGCCCGCGGCCACGATCAGCCAGATGGCACCAATAGCAGCGAGGACGGCACCGACCAGGTGGGTCCAGGCGTTGAAGCGTTCACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACCTGCTCACCCACCTGCACCGCTGCCAGCTCCAACCTGCCCAGCGCCACATCCACGTAGTTCAGCTGGCTGTCGAGCTTGCACGAGCGCGGAATGTCCTGCAGCAACAGCGCCAGGTAGCGCAAACCGGTATCGCCCAACGCATTGAGCACCACCA  >  AE015451/5681125‑5681715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.