| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 1,070,246 | 1 | . | G | 76.0% | 65.9 / 17.8 | 25 | intergenic (+46/+96) | aroP‑I/PP_0928 | aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger related‑protein |
| Reads supporting (aligned to +/- strand): ref base . (2/4); new base G (9/10); total (11/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAACTGGGCGAGCATCAGCATTACCCACCTCAAATTCCGCAAGGCCAAGCTGGCCGCCGGGATCACCCCCTTCTACAAAAGCCTGGGGCACCCGCTGACCAACTACCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GT‑‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATAGAACAGCATCAATCGCTCCAGGCGGCCAGCCAACGGCATACCCATGGTAAAAGCCGCCACATGAAGGCCGTAGGCCAGGTA > AE015451/1069951‑1070491 | cAACTGGGCGGGCATCAGCATTACCCACCTCAAATTCCGCAAGGCCAAGCTGGCCGCCGGGATCACCCCCTTCTACAAAAGCCTGGGGCACCCGCTGACCAACTACCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GT‑‑Gag < 2:603278/299‑2 (MQ=255) aaCTGGGCGAGCATCAGCATTACCCACCTCAAATTCCGCAAGGCCAAGCTGGCCGCCGGGATCACCCCCTTCTACAAAAGCCTGGGGCACCCGCTGACCAACTACCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCt‑‑gt‑‑g > 1:563776/1‑296 (MQ=255) gggCGAGCATCAGCATTACCCACCTCAAATTCCGCAAGGCCAAGCTGGCCGCCGGGATCACCCCCTTCTCCAAAAGCCTGGGGCACCCGCTGACCAACTACCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GT‑‑Gagatcga < 2:532611/299‑7 (MQ=255) gggCGAGCATCAGCATTACCCACCTCAAATTCCGCAAGGCCAAGCTGGCCGCCGGGATCACCCCCTTCTACAAAAGCCTGGGGCACCCGCTGACCAACTACCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GT‑‑Gagatcg > 1:398469/1‑293 (MQ=255) gCATCAGCATTACCCACCTCAAATTCCGCAAGGCCAAGCTGGCCGCCGGGATCACCCCCTTCTACAAAAGCCTGGGGCACCCGCTGACCAACTACCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGcgcc < 1:838446/298‑1 (MQ=255) aTCAGCATTACCCACCTCAAATTCCGCAAGGCCAAGCTGGCCGCCGGGATCACCCCCTTCTACAAAAGCCTGGGGCACCCGCTGACCAACTACCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCt‑‑gt‑‑g > 2:207234/1‑284 (MQ=255) aTTCCGCAAGGCCAAGCTGGCCGCCGGGATCACCCCCTTCTACAAAAGCCTGGGGCACCCGCTGACCAACTACCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GT‑‑Gagatc < 2:844505/269‑5 (MQ=255) ccGCAAGGCCAAGCTGGCCGCCGGGATCACCCCCTTCTACAAAAGCCTGGGGCACCCGCTGACCAACTACCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAgg < 1:240107/299‑1 (MQ=255) cAAGCTGGCCGCCGGGATCACCCCCTTCTACAAAAGCCTGGGGCACCCGCTGACCAACTACCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAg < 2:563755/299‑1 (MQ=255) aaGCTGGCCGCCGGGATCACCCCCTTCTACAAAAGCCTGGGGCACCCGCTGACCAACTACCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGcc < 1:207239/300‑1 (MQ=255) aCCCGCTGACCAACTACCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGCAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCAt > 1:571001/1‑299 (MQ=255) gACCAACTACCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTcc > 1:927450/1‑284 (MQ=255) aaCTACCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGcc < 1:274553/246‑1 (MQ=255) aaCTACCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGcc > 2:274546/1‑246 (MQ=255) aCCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGGGA‑‑GATCGAGCGCCGCGCGGGCGGCGCTCg > 2:927941/1‑221 (MQ=255) ggCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGcccc > 2:69787/1‑279 (MQ=255) cATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGgcggcg > 2:75890/1‑201 (MQ=255) cTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCa > 2:117815/1‑284 (MQ=255) gccgATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGGGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtg < 2:398454/268‑1 (MQ=255) aTGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACCCCGTAGCGAGCACCGGCAGGACATAGAACAGc > 1:313689/1‑280 (MQ=255) aTGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCCCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATAGAACAGCATCAATCGCTCCAgg > 1:373695/1‑295 (MQ=255) ctgtgctgtgGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCg > 2:26363/1‑211 (MQ=255) ctgtgctgtgGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCg < 1:26365/211‑1 (MQ=255) ggCTTTCAAGCTGAGGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGGTAGGTTTTCGGCGCCTGGGA‑‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATAGAACAGCATCAATCGCTCCAGGCGGCCAGCCAACGGCATACCCATGGt < 1:927967/300‑1 (MQ=255) aagaagGCTCGCCAGGCCAAGGGGTGATCGGGGTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGAGCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCTACACCGTAGCGAGCACCGGCAGTACATAGAACAGCATCAATCGCTCCAGGCGGCCAGCCAACGGCATACCCATGGTaaaa < 2:570979/291‑1 (MQ=255) ccAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATAGAACAGCATCAATCGCTCCAGGCGGCCAGCCAACGGCATACCCATGGTAAAAGCCGCCACATGAAGGCCGTa < 1:69789/300‑1 (MQ=255) ggCTGATCGGGGTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGGGCCT‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATAGAACAGCATCAATCGCTCCAGGCGGCCAGCCAACGGCATACCCATGGTAAAAGCCGCCACATGAAGGCCGTAGGCCAGGTa < 2:313681/299‑1 (MQ=255) | CAACTGGGCGAGCATCAGCATTACCCACCTCAAATTCCGCAAGGCCAAGCTGGCCGCCGGGATCACCCCCTTCTACAAAAGCCTGGGGCACCCGCTGACCAACTACCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCT‑‑GT‑‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATAGAACAGCATCAATCGCTCCAGGCGGCCAGCCAACGGCATACCCATGGTAAAAGCCGCCACATGAAGGCCGTAGGCCAGGTA > AE015451/1069951‑1070491 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |