Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451	2,958,523	C→T	A428V (GCA→GTA)	PP_2589 →	Aldehyde dehydrogenase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	2,958,523	0	C	T	100.0%	205.4 / NA	57	A428V (GCA→GTA)	PP_2589	Aldehyde dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/28);  total (29/28)

GGATCCGGCGAGCCGCGCCGGGGCCATTGTCGATGCCGGGCAGACCGGCCGCATCGAGGCCGCCATTGTGCGTGCCGGGCAAGAGGGCGCGCGATTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGCAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTT  >  AE015451/2958239‑2958809                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                               ggATCCGGCGAGCCGCGCCGGGGCCATTGTCGATGCCGGGCAGACCGGCCGCATCGAGGCCGCCATTGTGCGTGCCGGGCAAGAGGGCGCGCGATTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCTGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGc                                                                                                                                                                                                                                                                                <  1:865801/301‑1 (MQ=255)                   cGGGGCCATTGTCGATGCCGGGCAGACCGGCCGCATCGAGGCCGCCATTGTGCGTGCCGGGCAAGAGGGCGCGCGATTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTTCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGt                                                                                                                                                                                                                                                               >  1:32840/1‑300 (MQ=255)                         cATTGTCGATGCCGGGCAGACCGGCCGCATCGAGGCCGCCATTGTGCGTGCCGGGCAAGAGGGCGCGCGATTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCCTCTATGGCCTGGTAGCCTCGGTGTGGAGcgac                                                                                                                                                                                                                                                                             >  1:805879/1‑280 (MQ=255)                          aTTGTCGATGCCGGGCAGACCGGCCGCATCGAGGCCGCCATTGTGCGTGCCGGGCAAGAGGGCGCGCGATTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCggg                                                                                                                                                                                                                                                       >  1:609/1‑301 (MQ=255)                             gTCGATGCCGGGCAGACCTGCCGAATCGAGGCCGCCATTGTGCGTGCCGGGCAAGAGGGCGCGCGATTGGGGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGcgac                                                                                                                                                                                                                                                                             <  2:805853/276‑1 (MQ=255)                               cGATGCCGGGCAGACCGGCCGCATCGAGGCCGCCATTGTGCGTGCCGGGCAAGAGGGCGCGCGATTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAg                                                                                                                                                                                                                                                    <  1:917945/299‑1 (MQ=255)                                         cAGACCGGCCGCATCGAGGCCGCCATTGGGCGTGCCGGGCAAGAGGGCGCGCGATTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTg                                                                                                                                                                                                                                         <  2:62186/300‑1 (MQ=255)                                               ggCCGCATCGAGGCCGCCATTGTGCGTGCCGGGCAAGAGGGCGCGCGATTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCGCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACGGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCg                                                                                                                                                                                                                                            >  2:707238/1‑291 (MQ=255)                                               ggCCGCATCGAGGCCGCCATTGTGCGTGCCGGGCAAGAGGGCGCGCGATTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTTCGCCTGGCCAACGACAGCATCCATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCgg                                                                                                                                                                                                                                               >  2:62384/1‑288 (MQ=255)                                                              gccATTGTGCGTGCCGGGCAAGAGGGCGCGCGATTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTAt                                                                                                                                                                                                                         >  2:22871/1‑295 (MQ=255)                                                               ccATTGTGCGTGCCGGGCAAGAGGGCGCGCGATTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGaa                                                                                                                                                                                                                  <  1:707263/301‑1 (MQ=255)                                                                cATTGTGCGTGCCGGGCAAGAGGGCGCGCGATTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGttt                                                                                                                                                                                                                         <  2:32838/293‑3 (MQ=255)                                                                        gtgcCGGGCAAGAGGGCGCGCGATTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGATCGACGACTTCAACCAGGTGCACCGGGTACCGCGGGCGTTGAAAGCCGGTAcc                                                                                                                                                                                                                             >  1:963122/1‑281 (MQ=255)                                                                        gtgcCGGGCAAGAGGGCGCGCGATTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTCTCGGt                                                                                                                                                                                                                     >  1:618471/1‑289 (MQ=255)                                                                          gcCGGGCAAGAGGTCGCGCGATGGGTGTTGGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTCCATCGAGCCGACCAGTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAg                                                                                                                                                                                                             <  2:609/295‑1 (MQ=255)                                                                               gCAAGAGGGCGCGCGATTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCt                                                                                                                                                                                                   >  1:242109/1‑300 (MQ=255)                                                                                    agGGCGCGCGATTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGAtg                                                                                                                                                                                              <  1:22873/300‑1 (MQ=255)                                                                                              aTTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGAtgtg                                                                                                                                                                                            >  1:117958/1‑292 (MQ=255)                                                                                              aTTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTGGCCGGTTTCGAGGGCCGAATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGAt                                                                                                                                                                                                        <  1:969356/280‑1 (MQ=255)                                                                                                 ggtgggtgGCGGCCGAAGCCTGACCATCCAAGTTTCGGACAACTCAATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTc                                                                                                                                                                                <  2:618446/301‑1 (MQ=255)                                                                                                          cggcCGAGGCCTGACCATCGAAGGTTCGGACAACTCCATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTAcc                                                                                                                                                                                                                             <  2:963096/247‑1 (MQ=255)                                                                                                               gACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGt                                                                                                                                                                             >  2:500871/1‑290 (MQ=255)                                                                                                               gACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGt                                                                                                                                                                             <  1:500892/290‑1 (MQ=255)                                                                                                                  gCCTGACAATCGAAGGTTCGGCAAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTgg                                                                                                                                                                           <  2:701434/289‑1 (MQ=255)                                                                                                                                 gTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATTTGACGGTGCCGTTCGGTGGGGTCAAGCAGTCggg                                                                                                                                                            >  2:725101/1‑289 (MQ=255)                                                                                                                                            tACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCcg                                                                                                                                                   >  2:451832/1‑287 (MQ=255)                                                                                                                                                     ccGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCAttcgttc                                                                                                                             <  1:451852/300‑1 (MQ=255)                                                                                                                                                                    ggTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCt                                                                                                                                             >  1:283448/1‑269 (MQ=255)                                                                                                                                                                    ggTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGAAACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCt                                                                                                                                             <  2:283441/269‑1 (MQ=255)                                                                                                                                                                     gtgtCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTTAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTTGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCAttcgtt                                                                                                                              >  2:490790/1‑283 (MQ=255)                                                                                                                                                                          gAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTc                                                                                                           >  1:397516/1‑297 (MQ=255)                                                                                                                                                                          gAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGGCACTGCATTCGTTCGACAAGTATTCg                                                                                                                 >  1:898930/1‑291 (MQ=255)                                                                                                                                                                            gggCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCAttc                                                                                                                                 <  2:242103/273‑1 (MQ=255)                                                                                                                                                                              gCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGACAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGacc                                                                                                     >  1:876816/1‑299 (MQ=255)                                                                                                                                                                                   aTGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTgg                                                                                               >  1:938063/1‑300 (MQ=255)                                                                                                                                                                                              gcgcgAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTgcg                                                                                    <  1:109604/300‑1 (MQ=255)                                                                                                                                                                                                  gaggagGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGcc                                                                                                                                                    >  1:490151/1‑232 (MQ=255)                                                                                                                                                                                                  gaggagGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGt                                                                                              <  1:599736/286‑1 (MQ=255)                                                                                                                                                                                                  gaggagGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACATGGt                                                                                              >  2:599712/1‑286 (MQ=255)                                                                                                                                                                                                    ggaggTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTgc                                                                                     <  2:938037/293‑1 (MQ=255)                                                                                                                                                                                                          gtTCGGACCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTa                                                                         <  1:725127/299‑1 (MQ=255)                                                                                                                                                                                                                      aCTGGCGGTCAGCGCCTTCGTCACGGAAGAAGAAGCCGTTCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCTCGGCTGATGTATTGATCATTGAc                                                             <  1:166007/299‑1 (MQ=255)                                                                                                                                                                                                                      aCTGGCGGTCAGCGCCGTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGAcc                                                            <  2:876790/300‑1 (MQ=255)                                                                                                                                                                                                                           cGGTCAGCGCCTTCGACCCGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTg                                                                                                <  2:898904/259‑1 (MQ=255)                                                                                                                                                                                                                           cGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGcc                                                                                                                                                    <  2:490130/207‑1 (MQ=255)                                                                                                                                                                                                                                   gcCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCtt                                               >  2:636049/1‑300 (MQ=255)                                                                                                                                                                                                                                    ccTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTc                                              >  1:234877/1‑300 (MQ=255)                                                                                                                                                                                                                                        cGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGac                                                                                                      <  2:278129/240‑1 (MQ=255)                                                                                                                                                                                                                                                      aagCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTccc                            <  1:636074/300‑1 (MQ=255)                                                                                                                                                                                                                                                               gcCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTGCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTAGCGTATCGGGGAACACAGTCGATGCGCGGGATGTGACGGTGCCGGTCGGTGGGGGCAAGCGGTCGGGGGTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGCGGTATTGATCATTGACCGGGTCGTCTGCTTCTGGGTCATGCCCGATCCCACAGGTTc                    >  1:554467/1‑299 (MQ=255)                                                                                                                                                                                                                                                                   ggCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCAt                                      <  2:234872/277‑1 (MQ=255)                                                                                                                                                                                                                                                                     ccAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCt                                                >  2:413972/1‑265 (MQ=255)                                                                                                                                                                                                                                                                               cATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGtt  >  1:682137/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                ttCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCTACTCAAGac                                                                                                      >  1:643376/2‑200 (MQ=255)                                                                                                                                                                                                                                                                                aTCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGtt  >  1:517262/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    aTGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGcac               <  2:606550/283‑1 (MQ=255)                                                                                                                                                                                                                                                                                    aTGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGcac               >  1:606575/1‑283 (MQ=255)                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                               GGATCCGGCGAGCCGCGCCGGGGCCATTGTCGATGCCGGGCAGACCGGCCGCATCGAGGCCGCCATTGTGCGTGCCGGGCAAGAGGGCGCGCGATTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGCAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTT  >  AE015451/2958239‑2958809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑