Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451	4,889,556	G→A	intergenic (+2/‑151)	hyi → / → glxR	hydroxypyruvate isomerase/tartronate semialdehyde reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	4,889,556	0	G	A	100.0%	133.4 / NA	38	intergenic (+2/‑151)	hyi/glxR	hydroxypyruvate isomerase/tartronate semialdehyde reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/20);  total (18/20)

AAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTCCACCCACCACGACGCCGCGCCAGCCGACCTG  >  AE015451/4889259‑4889826                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                               aaaTCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAaac                                                                                                                                                                                                                                                                             <  1:137945/301‑1 (MQ=255)        ggCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCgg                                                                                                                                                                                                                                                                      <  2:780081/301‑1 (MQ=255)          ccGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCTGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCggg                                                                                                                                                                                                                                                                     <  2:559932/300‑1 (MQ=255)                   gCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTggg                                                                                                                                                                                                                                                           >  1:117254/1‑301 (MQ=255)                                   ccTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCCGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTggg                                                                                                                                                                                                                                                           >  2:864024/1‑285 (MQ=255)                                   ccTGCAAAACGACACCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTggg                                                                                                                                                                                                                                                           <  1:864050/285‑1 (MQ=255)                                       cAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAaac                                                                                                                                                                                                                                                                             <  1:963723/263‑1 (MQ=255)                                       cAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAaac                                                                                                                                                                                                                                                                             >  2:963697/1‑263 (MQ=255)                                       cAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAac                                                                                                                                                                                                                                        >  1:480331/1‑300 (MQ=255)                                                aTCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGAACACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTAGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCaa                                                                                                                                                                                                                                >  1:188522/1‑299 (MQ=255)                                                     ccACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACATGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAg                                                                                                                                                                                                                                                         <  1:745412/269‑1 (MQ=255)                                                                           gACCTGGCTCGAACCATGGCACCCAACCTGAAGCTGATCCACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTg                                                                                                                                                                                                     <  2:22491/299‑1 (MQ=255)                                                                              cTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTc                                                                                                                                                                                                 >  1:242250/1‑300 (MQ=255)                                                                                                 ccAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAaac                                                                                                                                                                                                                                                                             <  2:9340/205‑1 (MQ=255)                                                                                                 ccAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAaac                                                                                                                                                                                                                                                                             >  1:9342/1‑205 (MQ=255)                                                                                                                                               ccGCAATGAACCATGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAac                                                                                                                                                                                                                                        <  1:784995/196‑1 (MQ=255)                                                                                                                                                   aaTGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAAATACAAAGAGGTAAttt                                                                                                                             >  1:181912/1‑299 (MQ=255)                                                                                                                                                    aTGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTtct                                                                                                                           >  1:245628/1‑300 (MQ=255)                                                                                                                                                       aaCCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACAACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCAt                                                                                                                        <  2:480310/300‑1 (MQ=255)                                                                                                                                                                              tACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTtctc                                                                                                                          >  1:903018/1‑275 (MQ=255)                                                                                                                                                                                      ccTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGcatc                                                                                         >  1:717012/1‑300 (MQ=255)                                                                                                                                                                                      ccTGTTCGAACACCTGGACCGCATTGGCTACAAGGGCTGGGTGGGCGCCGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATc                                                                                                                                                  <  2:320917/243‑1 (MQ=255)                                                                                                                                                                                         gTTCGAACACCGGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTtctc                                                                                                                          <  2:902992/264‑1 (MQ=255)                                                                                                                                                                                                cacCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAAt                                                                                                                                             >  1:721234/1‑238 (MQ=255)                                                                                                                                                                                                cacCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAAt                                                                                                                                             <  2:721208/238‑1 (MQ=255)                                                                                                                                                                                                      gACCGCATTGGCTACCAGGGCTGAGTGTGCGCGGATTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGTGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATgg                                                                         <  2:188518/300‑1 (MQ=255)                                                                                                                                                                                                                       gggCTGGGTGGGAGCGGAGTAAAAGCCACTGACCACAACCGAAGCGGGCCTGGGCTGGCGGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAgg                                                       <  2:242244/301‑1 (MQ=255)                                                                                                                                                                                                                                gggCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGCGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAg                                                    <  1:120074/295‑1 (MQ=255)                                                                                                                                                                                                                                gggCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCAt                                                                           <  2:408296/272‑1 (MQ=255)                                                                                                                                                                                                                                gggCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCAt                                                                           >  1:408315/1‑272 (MQ=255)                                                                                                                                                                                                                                        aGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATg                                                                          <  2:44874/265‑1 (MQ=255)                                                                                                                                                                                                                                        aGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATg                                                                          >  1:44876/1‑265 (MQ=255)                                                                                                                                                                                                                                         gTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTc                                      >  1:888832/1‑300 (MQ=255)                                                                                                                                                                                                                                           aCAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATc                                                                                                     >  1:201946/1‑235 (MQ=255)                                                                                                                                                                                                                                           aCAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATc                                                                                                     <  2:201941/235‑1 (MQ=255)                                                                                                                                                                                                                                                               gAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTAGCCCGCGAACACCGGCAAAGCAGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACCGGGGGACAAAGCTTGCTTGAGATATCACAATTACaa                                                                                                                                        >  2:41932/1‑180 (MQ=255)                                                                                                                                                                                                                                                                           ggctggctGAAAACCCACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTccaccc                            <  2:245622/276‑1 (MQ=255)                                                                                                                                                                                                                                                                                        cccACAACGCAATCTGAGAAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTCCACCCACCACGACGCCGCGCCAGCCGACCTg  >  2:37757/1‑289 (MQ=255)                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                               AAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAACCACATCCAGCTGGCCGACAACCCAGGCCGCAATGAACCAGGCACCGGCGAGATCAACTACCGCTTCCTGTTCGAACACCTGGACCGCATTGGCTACCAGGGCTGGGTGGGCGCGGAGTACAAGCCGCTGACCACCACCGAAGCGGGCCTGGGCTGGCTGAAAACCCACAACGCAATCTGAGGAACAACTCGGGACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTCCACCCACCACGACGCCGCGCCAGCCGACCTG  >  AE015451/4889259‑4889826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑