Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451 1,499,480 2 bp→AC intergenic (+57/‑106) trpS → / → zapE Tryptophan‑‑tRNA ligase/putative enzyme with nucleoside triphosphate hydrolase domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154511,499,4800CA97.4% 131.4 / NA 39intergenic (+57/‑107)trpS/zapETryptophan‑‑tRNA ligase/putative enzyme with nucleoside triphosphate hydrolase domain
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (18/20);  total (19/20)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*AE0154511,499,4810AC100.0% 130.7 / NA 39intergenic (+58/‑106)trpS/zapETryptophan‑‑tRNA ligase/putative enzyme with nucleoside triphosphate hydrolase domain
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (19/20);  total (19/20)

TGATGGCGAGCAACTGCTGTTGTCGCGCAGCTTCGCCGACGGCAAGAGTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCAC‑G‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAACAAGCCGGGCATGTTCGGCAAGCTGTTCGGCAAGAAGGAGCAGACTC  >  AE015451/1499192‑1499758
                                                                                                                                                                                                                                                                                                ||                                                                                                                                                                                                                                                                                         
tGATGGCGAGCAACTGCTGTTGTCGCGCAGCTTCGCCGACGGCAAGAGTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTTCATGGCACCGGCTTTGcc                                                                                                                                                                                                                                                                                 >  1:143233/1‑300 (MQ=255)
                             gCTTCGCCGACGGCAAGAGTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAc‑g‑ccc                                                                                                                                                                                                                                                            <  2:70467/290‑2 (MQ=255)
                                        ggCAAGAGTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCa                                                                                                                                                                                                                                       <  1:63720/300‑1 (MQ=255)
                                               gTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCTCCCACAg                                                                                                                                                                                                                                                  <  2:143230/282‑1 (MQ=255)
                                                                aagtagcTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTg                                                                                                                                                                                                               <  1:520606/296‑1 (MQ=255)
                                                                aagcaGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGt                                                                                                                                                                                                                <  1:620229/299‑1 (MQ=255)
                                                                      cTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGCGTTCGCGGGCCC‑GCCC‑GCCCCCACACACCGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGccc                                                                                                                                                                                                        >  1:557827/1‑301 (MQ=255)
                                                                        gcagtaAGGTGGCGAGGTCGATGGCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGtt                                                                                                                                                                                                                                                                           <  2:566107/234‑1 (MQ=255)
                                                                        gcagaaAGGTGGCAAGGCCGATGTCCGAATCGAAGGCCTGAGCTTCAGCCTGTGGCTCAATGGCGAGCAGGTTACCAACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCAGCCATTCAGAGCCTGAACGAAGCCTTGGAACCACAGCAGGACTGAGGCTGCCAAACGGTGGGGGCGGGCGTACCCGCGAAACAGGCACCACGGTGCATAGCACCGGCTTTGCCAGTGTTCGCGGGCACAC‑CC‑GCTCCCACAGACTGATTACCATTAAGCGGGCCTATCGCTACAGTGACGGccc                                                                                                                                                                                                        <  1:417507/299‑1 (MQ=255)
                                                                           gcaAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTa                                                                                                                                                                                                                          <  2:197025/278‑1 (MQ=255)
                                                                           gcaAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCCCCGCCGTGCATGGCACCAGCTTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCCCCCACAGACTGATTGCCATTAAGCGGGCCt                                                                                                                                                                                                                           >  1:197029/1‑277 (MQ=255)
                                                                                       ggCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCg                                                                                                                                                                                        >  1:754107/1‑300 (MQ=255)
                                                                                          cGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCt                                                                                                                                                                                                                     >  1:192452/1‑268 (MQ=255)
                                                                                                  gCATCGAAGGCCTGGGCTTCGGCATGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCg                                                                                                                                                                                        <  2:192448/289‑1 (MQ=255)
                                                                                                    aTCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCTCCcaca                                                                                                                                                                                                                                                   <  2:299416/228‑1 (MQ=39)
                                                                                                      cGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCCCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCTCCCACCGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTTACGGCCCGTTTTTTGTTGCCTCGc                                                                                                                                                                                       >  2:393062/1‑286 (MQ=255)
                                                                                                       gAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCTCCcac                                                                                                                                                                                                                                                    >  1:829946/1‑224 (MQ=38)
                                                                                                             cTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCTACCACAGACTGCTTGcc                                                                                                                                                                                                                                        >  1:863053/1‑230 (MQ=38)
                                                                                                                     cGGCCGGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa                                                                                                                                                          <  1:760523/300‑1 (MQ=255)
                                                                                                                                                          ccGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAcg                                                                                                                    <  1:830733/301‑1 (MQ=255)
                                                                                                                                                               gTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTTCATGGCACCGGCTTTGCTGGTTTTCGCGGGCAC‑GCCC‑GCCCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACa                                                                                                                                                                                                                  >  2:779993/1‑202 (MQ=255)
                                                                                                                                                               gTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACa                                                                                                                                                                                                                  <  1:780019/202‑1 (MQ=38)
                                                                                                                                                                     cAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCTACTTCTTCCATGAcgc                                                                                                                   >  1:530289/1‑291 (MQ=255)
                                                                                                                                                                         gCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTAGCGGGCAC‑GCCC‑GCTCCCACAGACAGATTGCCATTAAGCGGGCCGATCg                                                                                                                                                                                                                       >  1:382129/1‑187 (MQ=255)
                                                                                                                                                                                          ggCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGCAACAGGCGCCGCGGTGCATGGCACCGGCGTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCCCCCACAGACTGACGGCCATTAAGCGGGCCTAACGCCACAGTGAc                                                                                                                                                                                                             >  2:837715/1‑180 (MQ=255)
                                                                                                                                                                                              aTTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGc                                                                                                                                                    >  2:770034/1‑233 (MQ=39)
                                                                                                                                                                                              aTTCAGAGCCTGAACGAAGCCTTGGAACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATg                                                                                                                       <  1:770060/262‑1 (MQ=39)
                                                                                                                                                                                                      ccTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCt                                                                                        <  2:754081/285‑1 (MQ=255)
                                                                                                                                                                                                             gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGGGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAg                                                                                                            <  2:817530/258‑1 (MQ=39)
                                                                                                                                                                                                                  cTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGGGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTcc                                                                                                                                                                      >  2:617890/1‑195 (MQ=38)
                                                                                                                                                                                                                     ggCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATTACTCCCTTAGAACGCt                                                                                                                                                           >  2:872950/1‑203 (MQ=38)
                                                                                                                                                                                                                            cagcagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGCGTTCGCGGGCAC‑G‑CCTGCCCCCACAGACTGATTGCCATTAAGCGGGCCTATAGCTACAGTGCAGGCCCGTTTTTTGTTGCCTCGCTAACGACTCATGACTCCCTTAGAACg                                                                                                                                                             >  1:408768/1‑194 (MQ=255)
                                                                                                                                                                                                                                agGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCa                                                                                                                         <  2:758558/226‑1 (MQ=39)
                                                                                                                                                                                                                                agGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCCAACGAATCATGACTCCCTTAGAACGCTATCCAGCAGATCTGAAAc                                                                                                                                         >  1:758584/1‑210 (MQ=38)
                                                                                                                                                                                                                                agGACTGAGGCCGCCAAACGGTGGGAGCGGGCGTGCCCGCGCAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCTCACACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAAc                                                                                                                                                                                   <  2:257485/168‑1 (MQ=37)
                                                                                                                                                                                                                                                      gggAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCCTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGATTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAATGTCCCGACTTCTTCCATg                                                                                                                       >  1:943616/1‑206 (MQ=38)
                                                                                                                                                                                                                                                                  gCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGt                                                                                                 <  2:382117/216‑1 (MQ=39)
                                                                                                                                                                                                                                                                                      ggTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTTCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAACAAGCCGGGCATGTTCGGCAAGCTGTTCGGCAAGAAGGAGCAGACTc  >  1:907036/1‑291 (MQ=255)
                                                                                                                                                                                                                                                                                         gCATGGCACCGGCTTTGCCGGGGTTCGCGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAACAAGCCGGGCATGTTCGGCAAGCTGTTCGGCAAGAAGGAGCAGACTc  <  2:907010/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                ||                                                                                                                                                                                                                                                                                         
TGATGGCGAGCAACTGCTGTTGTCGCGCAGCTTCGCCGACGGCAAGAGTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCAC‑G‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAACAAGCCGGGCATGTTCGGCAAGCTGTTCGGCAAGAAGGAGCAGACTC  >  AE015451/1499192‑1499758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑