Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451	1,932,640	+C	intergenic (‑107/+43)	rluA ← / ← minE	23S rRNA (746) and tRNA (32) pseudouridine synthase/Cell division topological specificity factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	1,932,638	1	.	C	100.0%	61.0 / NA	18	intergenic (‑105/+45)	rluA/minE	23S rRNA (746) and tRNA (32) pseudouridine synthase/Cell division topological specificity factor
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (9/9);  total (9/9)

TTCCCAGTCCAGACGGTGCACGATCAGCGCATCGGGGTAGCCGTTTTCCTGCAGACGGGTGATCAGGCAGTCCTTGTTGTCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCAC‑GAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATGATCTGTAGACGCTCTTTCGCTACCGACGCGCTGGTCTGTTTCTGTCTGCCACGAA  >  AE015451/1932357‑1932918                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                         ttCCCAGTCCAGACGGTGCACGATCAGGGCATCGGGGTAGCCGTTTTCCTGCAGGCGGGTGATCAGGCAGTCCTTGTTGTCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCAC‑GAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAgg                                                                                                                                                                                                                                                                        <  2:744111/301‑1 (MQ=255)          cAGACGGTGCACGATCAGCGCATCGGGGTAGCCGTTTTCCTGCAGACGGGTGATCAGGCAGTCCTTGTTGTCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCGCAC‑GAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCaa                                                                                                                                                                                                                                                               <  1:841329/301‑1 (MQ=255)                gTGCACGATCAGCGCATCGGGGTAGCCGTTTTCCTGCAGACGGGTGATCAGGCAGTCCTTGTTGTCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCAC‑GAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCCAGCCCGATCCTACAAAGGCTATGGGTCCCCTGTAGTAGCGGGCTTGCCCGCGAAGGGCTGCCAGGCAGCCCCAAATTGTc                                                                                                                                                                                                                                                         >  1:339081/1‑301 (MQ=255)                              gttCGGGGTAGCCCGTTTCCTGCAGAAGGGTGATTAGGCAGGCCTGGTGGTCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCAC‑GAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCa                                                                                                                                                                                                                                           <  2:445985/299‑1 (MQ=255)                                                                             ttgtCCTCGGCACGGCAAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCAC‑GAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCt                                                                                                                                                                                            <  1:454614/301‑1 (MQ=255)                                                                               gtCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCAC‑GAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCCGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTgg                                                                                                                                                                                          >  1:374806/1‑301 (MQ=255)                                                                                                                 gtggggttgttgggaaCCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCAC‑GAAAATAGCAGTAAACGGTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGa                                                                                                                                                        <  2:745646/286‑1 (MQ=255)                                                                                                                          ttgtgcACCAGGATGGCGGCGTCTACGAAGAGGATCGGGACATTCGCCAGCAGCATGGCAGGTCT‑ACAGAAAATAGCAGGAACCGCTGGGGCCGGTTCGCGGCCCTTCGCGGGCAAGCCCGCGCCTGCAAAGGATATGCGTCGCCTGGAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACat                                                                                                                                               <  2:658970/296‑1 (MQ=255)                                                                                                                                                         gtatctggacgtgcgggtggggCATGGCAGGCTTCAC‑GAAAATAGCAGTGAACGATGGGGCCGCTGTGCGGCCCTTCGCGGGCAAGCCCGATCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGATTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTg                                                                                                                <  2:228956/281‑1 (MQ=255)                                                                                                                                                                             ggCATTGCAGGTCTCAC‑GAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCCCGATATGCACATCCTCGTTGACGATGTTCACATATTTTCGGATCACTTCCAGACGTTCTTTCTGCAGCGCGGGCAGGTAGTCCg                                                                                            >  2:517871/1‑301 (MQ=255)                                                                                                                                                                                    cAGGTCTCAC‑GAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGTGCGGGCAGGTAGTCCGGTTCGCt                                                                                     >  1:865822/1‑301 (MQ=255)                                                                                                                                                                                          tcAC‑GAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACCTATTTGCGGATCACTTCCAGCATTTCTTTCTGCAGCGCGGGCCGGTAGTCCGGTTCGCTGCGttt                                                                               >  1:545518/1‑300 (MQ=255)                                                                                                                                                                                              cgcaaaTCGCAGTAACCGCTGGCGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACCAAGGATATGCGTCCCCTGTAGGCGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAGTCGAGCAGCTTCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGcc                                                                            <  2:860549/298‑1 (MQ=255)                                                                                                                                                                                                                                    gatctgggCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATGATCTGTAGACGCTCTTTCg                                      <  1:683475/296‑1 (MQ=255)                                                                                                                                                                                                                                         gggCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGCTCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGCTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATGATCTGTAGACTCTCtttagagatc                                 >  2:683238/1‑294 (MQ=255)                                                                                                                                                                                                                                                                atatGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATGATCTGGAGACGCTCTTTCGCTTCCGACGCGCCGGTCTGTTtctgtct          >  2:181955/1‑301 (MQ=255)                                                                                                                                                                                                                                                                  atGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATGATCTGTAGACGCTCTTTCGCTACCGACGCGCTGGTCTGTTTCTGTCTGc        >  1:226839/1‑301 (MQ=255)                                                                                                                                                                                                                                                                        ccccTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCCGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGCTCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATGATCTGTAGACGCTCGTTCGCTACCGACGCGCTGGTCTGTTTCTGTCTGCCACGaa  >  1:643043/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                         TTCCCAGTCCAGACGGTGCACGATCAGCGCATCGGGGTAGCCGTTTTCCTGCAGACGGGTGATCAGGCAGTCCTTGTTGTCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCAC‑GAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATGATCTGTAGACGCTCTTTCGCTACCGACGCGCTGGTCTGTTTCTGTCTGCCACGAA  >  AE015451/1932357‑1932918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑