Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451	1,419,549	+C	intergenic (‑38/+98)	PP_1242 ← / ← PP_1244	conserved protein of unknown function/conserved protein of unknown function

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	1,419,548	1	.	C	100.0%	46.9 / NA	15	intergenic (‑37/+99)	PP_1242/PP_1244	conserved protein of unknown function/conserved protein of unknown function
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (10/5);  total (10/5)

CCCAACTCGGCATCATCCAGCAGCGCCTTGGCCATCGCGCTCAGGTAATGCGAGGCCCAGATCAGTTTCTGGTCGCCGTCCATCAGGCCGGTGATGATCAGGTCGCGCACGTAACCCATCAGTTCGGAAGCCTGTTCGCGGGCGTCCTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGG‑CTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTAAATGCGACCGCATCTGCAACTGCATCAACGCCGACTCGACCAGCCCCTGTACCGCTTCCATTTCATGGATCACGGCCTGCATCACGATCGAGCTGTGGGCGGTGGGTTTGAGTTGCACGGATTGGCGGGCCACGGACAGGGCGCAGAGT  >  AE015451/1419255‑1419824                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                     cccACCTCCGCATCATCCAGCAGCGCCTTGGCCATCGCGCTCAGGTAATGCGAGGCCCAGATCAGTTTCTGGTCGCCGCCCATCAGGCCGGTGATGATCAGGTCGCGCACGTAACCCATCAGTTCGGAAGCCTGTTCGCGGGCGTCCTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTc                                                                                                                                                                                                                                                                                <  1:363936/301‑1 (MQ=255)        cGGCATCATCCAGCAGCGCCTTGGCCATCGCGCTCAGGTAATGCGAGGCCCAGATCAGTTTCTGGTCGCCGTCCATCAGGCCGGTGATGATCAGGTCGCGCACGTAACCCATCAGTTCGGAAGCCTGTTCGCGGGCGTCCTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCGGGGCTGGCCTCTTCGCGGGcc                                                                                                                                                                                                                                                                         >  2:820104/1‑300 (MQ=255)            atcatcCAGCAGCGCCTTGGCCATCGCGCTCAGGTAATGCGAGGCCCAGATCAGTTTCTGGTCGCCGTCCATCAGGCCGGTGATGATCAGGTCGCGCACGTAACCCATCAGTTCGGAAGCCTGTTCGCGGGCGTCCTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGcc                                                                                                                                                                                                                                                                     <  1:864491/301‑1 (MQ=255)                tcCAGCAGCGCCTTGGCCATCGCGCTCAGGTAATGCGAGGCCCAGATCAGTTTCTGGTCGCCGTCCATCAGGCCGGTGATGATCAGGTCGCGCACGTAACCCATCAGTTCGGAAGCCTGTTCGCGGGCGTCCTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGGGTTGTTTTCGCCTTGGTAGAGGCAGGTCTTGCCGACGGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCt                                                                                                                                                                                                                                                                 >  2:526156/1‑301 (MQ=255)                      agcGCCTTGGCCATCGCGCTCAGGTAATGCGAGGCCCAGATCAGTTTCTGGTCGCCGTCCATCAGGCCGGTGATGATCAGGTCGCGCACGTAACCCATCAGTTCGGAAGCCTGTTCGCGGGCGTCCTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCcaca                                                                                                                                                                                                                                                           <  1:526331/301‑1 (MQ=255)                               gCCATCGCGCTCAGGTAATGCGAGGCCCAGATCAGTTTCTGGTCGCCGTCCATCAGGCCGGTGATGATCAGGTCGCGCACGTAACCCATCAGTTCGGAAGCCTGTTCGCGGGCGTCCTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACtgtg                                                                                                                                                                                                                                                  >  1:351316/1‑301 (MQ=255)                                ccATCGCGCTCAGGTAATGCGAGGCCCAGATCAGTTTCTGGTCGCCGTCCATCAGGCCGGTGATGATCAGGTCGCGCACGTAACCCATCAGTTCGGAAGCCTGTTCGCGGGCGTCCTGGCAGGGGATACCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTTGGCATTGTTTTGTTTCCCTGCATTCTGGGTTGCCGGGCCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGc                                                                                                                                                                                                                                                 >  2:319139/1‑301 (MQ=255)                                     gagcTCAGGTAATGCGAGGCCCAGATCAGTTTCTGGTCGCCGTCCATCAGGCCGGGGATGATCAGGTCGCGCACGTAACCCATCAGTTCGGAAGCCTGTTCGCGGGCGTCCTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGc                                                                                                                                                                                                                                            <  2:428746/299‑1 (MQ=255)                                        cTCAGGTAATGCGAGGCCCCGATCAGTTTCTGGTCGCCGTCCATCAGGCCGGTGATGATCAGGTCGCGCACGTAACCCATCAGTTCGGAAGCCTGTTCGCGGGCGTCCTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTg                                                                                                                                                                                                                                         <  2:238006/301‑1 (MQ=255)                                                                                                         gcgcACGTAACCCATCAGTTCGGAAGCCTGTTCGCGGGCGTCCTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACCGGTACTGTGCAGTGCGTGACGTCTGTGGTTACCCTGTGGAAGCGGGAGTGCCAGCGAAGAGGCCTgcccggcaagtgcgaggt                                                                                                                                                                        >  2:307487/1‑284 (MQ=255)                                                                                                                            tCGGAAGCCTGTTCGCGGGCGTCCTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTaaa                                                                                                                                                     >  1:834753/1‑301 (MQ=255)                                                                                                                                                       cAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGGCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGGAGAGGCCTGACCTGCTAATGCAAGGTGGTAGGCCGTGTGGGTAAATGCGACCGCAGCTGAAAGTGCATCaga                                                                                                                          >  2:59981/1‑299 (MQ=255)                                                                                                                                                                                                                                             tgttgtgttgTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTAAATGCGACCGCATCTGCAACTGCATCAACGCCGACTCGACCAGCCCCTGTACCGCTTCCATTTCATGGATCACGGCCTGCATCAGGATCGAGCTGTGGGCGGTGGGTTTGAGTTg                                    >  1:86383/1‑301 (MQ=255)                                                                                                                                                                                                                                                                            cccTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTAAATGCGACCGCATCTGCAACTGCATCAACGCCGACTCGACCAGCCCCTGTACCGCTTCCATTTCATGGATCACGGCCTGCATCACGATCGAGCTGTGGGCGGTGGGTTTGAGTTGCACGGATTGGCGGGCCACGGACAGGGCGCag     >  1:362972/1‑301 (MQ=255)                                                                                                                                                                                                                                                                               tGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTAGATTCGACCGCATCTGCAACTGCATCAACGCCGACTCGACCAGCCCCTGTACCGCTTCCATTTCATGGATCACGGCCTGCATCACGATCGAGCTGTGGGCGGTGGGTTTGAGTTGCACGGATTGGCGGGCCACGGACAGGGCGCagcgg  >  1:713007/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                     CCCAACTCGGCATCATCCAGCAGCGCCTTGGCCATCGCGCTCAGGTAATGCGAGGCCCAGATCAGTTTCTGGTCGCCGTCCATCAGGCCGGTGATGATCAGGTCGCGCACGTAACCCATCAGTTCGGAAGCCTGTTCGCGGGCGTCCTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGG‑CTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTAAATGCGACCGCATCTGCAACTGCATCAACGCCGACTCGACCAGCCCCTGTACCGCTTCCATTTCATGGATCACGGCCTGCATCACGATCGAGCTGTGGGCGGTGGGTTTGAGTTGCACGGATTGGCGGGCCACGGACAGGGCGCAGAGT  >  AE015451/1419255‑1419824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑