Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	AE015451	4,980,585	+GGC	intergenic (‑24/+44)	PP_4387 ← / ← flgE	conserved exported protein of unknown function/Flagellar hook protein FlgE

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 4980585	0 (0.000)	50 (0.740) +GGC	48/594	0.3	100%	intergenic (‑24/+44)	PP_4387/flgE	conserved exported protein of unknown function/Flagellar hook protein FlgE
	?	AE015451	4980586 =	0 (0.000)					intergenic (‑25/+43)	PP_4387/flgE	conserved exported protein of unknown function/Flagellar hook protein FlgE

CCGGTTTACGGGGCTTTCAGCGCGGCAAGGGGCAATAACCGT‑CAGTTTGTCGGCGGATGTTTGCCGCCTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/4980286‑4980585 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACC‑GGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGGCACGCCCGAGGCATAGGACT‑CTTTCCAGTTGGTACCGCCAATCGGCGTCAGGCCCTGCAGGTTGGCGAAGT  >  AE015451/4980586‑4980885                                                                                                                                                                                                                                                                                                              |||                                                                                                                                                                                                                                                                                                               CCGGTTTACGGGGCTTTCAGCGCGGCAAGGGGCAATAACCGT‑CAGTTTGTCGGCGGATGTTTGCCGCCTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGG                                                                                                                                                                                                                                                                                                                  <  1:665040/301‑1    ttttaagcgactgtacGCGCGGCTGGGGTCAATAACCGT‑CTGTTTGTCGGCGGATGTTGGCCGCCTGCGGCGAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCC                                                                                                                                                                                                                                                                                                               <  2:115746/285‑1    GTTTACGGGGCTTTCAGCGCGGCAAGGGGCAATAACCGT‑CAGTTTGTCGGCGGATGTTTGCCGCCTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGCGAACCTTCACATTCTTGGGGCC                                                                                                                                                                                                                                                                                                               >  1:806208/1‑301      TTACGGGGATTTCAGCGCGGCAAGGGGCAATAACCTT‑GAGTTTATCGGCGGGTGTTGGCCGCGTGCGGCAAGGGCTTGCTGAGTGGGCGGGGCGGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGAACCCAGCCTTTGACCGGGTTGTGGGCCGAACACAGGTAGCGGCTGGTGACCAGGCTTTGCCATGGAGAGAACGGAGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGC                                                                                                                                                                                                                                                                                                             <  2:860101/301‑1        cCGGGGCGTGCAGCGCGGCAAGGGGCAATAACCGT‑CAGTTTGGCGGCGGATGTTTGCCGCCTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGGAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTT                                                                                                                                                                                                                                                                                                           <  2:49771/300‑1         gGGGGCTTTCAGCGCGGCAAGGGTCAATAACCGT‑CAGTTTGTCGGCGGATGTTTGCCGCCTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTT                                                                                                                                                                                                                                                                                                          <  1:593913/300‑1          GGGGCTCTCAGGGCGTCAAGGGGCAACAA‑CGTGTAGTTGGTCGGCGGATGTTTGCCGCTGGCGGCAAGGGTTTGCTCAATGGGCGGGGCGGGCGGCTTTGTTGTAGGGGCCAGGGGGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTG                                                                                                                                                                                                                                                                                                         <  2:184345/301‑1                  CAGCGCGGCAAGGGGCAATAACCGT‑CAGTTTGTCGGCGGATGTTTGCCGCCTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGCTGTAGGGGCCAGAGTGGCGCCCCCAGCCCTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGCACCCGCACCCGCAAGGCGAACAAGGCCCGTAACGACAGGCGCATCATTCGTGTGCACCCTCGCATTCCTGGCGCCGCTTTcccggccac                                                                                                                                                                                                                                                                                                 >  2:29622/1‑292                    gcgggcCAAGGGGCAAGAGGCGT‑CAGTTTGTGGGCGGATGTGTGACGCTGGCGGCAAGGGCTTGCGCCATGGGTGCGGCAGGCGGCGTTGTGGTAGGGGCGAGAGTGGCGCACGCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAAT                                                                                                                                                                                                                                                                                               <  2:411418/295‑1                     gGCGGCAAGGGGAAATAACCGT‑CAGTTTGTCGGCGGATGTTTGCCGCCGGCGGGAAGGGCTGGCTCAAGGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATT                                                                                                                                                                                                                                                                                              <  2:32851/300‑1                       CGGCAAGGGGCAATAACCGT‑CAGTTTGTCGGCGGATGTTTGCCGCCTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGTCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTTGGCCGCACACAGGTCGCGGCCCGCGACCAGGCTTTGCCATCGATAGAACGCGGCGGGGGCCGCGGGGGCAGGCAGAACCAGCAACAGCAAGGGGAACAAGGCCAGTAACGACCGCCGCTCCATCCGCGTGAACCTTCCCATTCTTGGGGCCCCTTTGCGGCCCCACTTAC                                                                                                                                                                                                                                                                                            >  2:824244/1‑301                       CGGCAAGGGGCAATAACCGT‑CAGTTTGTCGGCGGATGTTTGCCGCCTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGAGTGAACCTTCACATTCTTGGCGCCGCATTGCGcacccacttaa                                                                                                                                                                                                                                                                                            >  1:357976/1‑290                                   aatcaccgtacGTTTGTGGGCGGCTGTTTGCCGCCTGCGGGAAGGGCTTGCTCTAGGGGCGCGGCAGGCGGAGTTGTTGTAGGGGACAGAGTGGCGCACCCAGCCTGTGCCCGGAGTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAAGCTTTGCCATTGATAGACCGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAG                                                                                                                                                                                                                                                                                 <  2:115496/290‑1                                      ACCGT‑CAGTTTGTCGGCGGATGTTTGCCGCCTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGACAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCGCAATGACAAACACCAAAGACAA                                                                                                                                                                                                                                                                             >  2:593076/1‑301                                        CGT‑CAGTTTGTCGGCGGATGTTTGCCGCCTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATA                                                                                                                                                                                                                                                                           >  1:647554/1‑301                                            CAGTTGGTCGGCGGAGGTTTGCGGCCGGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAA                                                                                                                                                                                                                                                                        <  1:520613/301‑1                                                    CGGCGGATGTTTGCCGCCTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGG                                                                                                                                                                                                                                                                >  1:375257/1‑301                                                                  gGCCTGCGGCAAGGGCTTGCTCAATGGGGGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATG                                                                                                                                                                                                                                                  <  1:563111/300‑1                                                                               GGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCCAAGCCAATACAATCATCAGGTCAaatgaacggtggtccgactattgg                                                                                                                                                                                                                                     >  2:123017/1‑277                                                                                 cttggcCAATGGCCGCGGCGGGCGGCGTTGTTGTAGGGGCCAGGGTGGCGCACCCAGCCTTTCCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTG                                                                                                                                                                                                                                   <  2:357863/295‑1                                                                                     CTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTGGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTT                                                                                                                                                                                                                               <  1:145166/301‑1                                                                                        aagGGGCGCGGAAGGGGGCGTGGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGACATTGATAGAACGGGGCGGGGGCGGCGGGGGCAGGCAGAACCAGAACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAG                                                                                                                                                                                                                            <  1:700631/298‑1                                                                                        AATGGGGGCGGCAGGAGGGGTTGTTGTAGGGGCCAGAGTGGCGAACCCAGCGTTTGCCCGGGTTGTGGGGCGAACACAGGTGGCGGCCGGTGACGAGGGTGTGCCATTGATAGAACGGGGCGGGGGGAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCGTGGGGCCGCTTTGCGGCGCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAG                                                                                                                                                                                                                            <  2:860530/301‑1                                                                                                   cgggcgggcTTGTTGTAGGGGCCAGGGTGGCGCCCCCAGCCTTGGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTC                                                                                                                                                                                                                 <  2:647331/292‑1                                                                                                      GCGGGGTTGTTGTAGGGGCCAGAGTGGCGCACCAAGCCGCGGCCCGGGGTGTGGGCCGAAAGCAGGGAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTG                                                                                                                                                                                                              <  2:214078/301‑1                                                                                                            TTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTG                                                                                                                                                                                                        <  1:169030/301‑1                                                                                                               TTGTAGGGGCCAGAGTGGCGAAACCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCC                                                                                                                                                                                                     <  1:131620/301‑1                                                                                                                         CAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGt                                                                                                                                                                                           >  1:139549/1‑300                                                                                                                                         GCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGACAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAG                                                                                                                                                                           <  1:400856/301‑1                                                                                                                                                  gGGGTTTTGGGTCGAACACAGGTAGCGGCCGGTTACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAG                                                                                                                                                                  <  1:422176/300‑1                                                                                                                                                  CGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCCAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGACATGGTCTTGGCATTTGCCTGGTAGTTGCTCTGCGCTTTGATCAGGTTTCCCAa                                                                                                                                                                  >  2:709090/1‑300                                                                                                                                                   GGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGTAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCACTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCCAATCCCATACAATCACCAAGTCATCTGAATCAAGGTCTGCATGATGGTGCTTACAGTGGTCCTGGTCTTGGCATTGGCGTGCTAGTTGCTCTGCTCCTTGATCAGTTTTACCcgc                                                                                                                                                                 >  2:405562/1‑298                                                                                                                                                               aagaacagggatgcGCCGTTGACCAGGCTTTGCCTTTGATAGAACGTGGCGGAGGACGCTGTGTCAGGCCGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGG                                                                                                                                                     <  2:465563/287‑1                                                                                                                                                                 ACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTAGTAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCAGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTC                                                                                                                                                   <  1:46463/301‑1                                                                                                                                                                       ggaggGGCCGGTGGCCAGGATTGGCGATGGAGAGGACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACAGTCTTGGGGCCGCTTGGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTT                                                                                                                                             <  2:732864/296‑1                                                                                                                                                                                GGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTC                                                                                                                                    >  1:486062/1‑301                                                                                                                                                                                                  TTGATTGAACGGGGAGGGGTCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACC‑GGAAAT                                                                                                                 <  2:139509/301‑1                                                                                                                                                                                                    GATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGATGCCTCCAGGGCACCACC‑GGCAATCT                                                                                                               >  1:2926/1‑301                                                                                                                                                                                                                  GGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCAGTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACC‑GGAAATCTGGCCGAGGGTACCG                                                                                                 <  1:824534/301‑1                                                                                                                                                                                                                       AGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTACTCCAGGGCACCACC‑GGAACTCTGGCCGAGGGTACCGGTGTC                                                                                            >  2:317636/1‑301                                                                                                                                                                                                                                 GGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACC‑GGAAATCTGGCCGAGGGTACCGGTGTCCGGACCGCCc                                                                                  >  1:129616/1‑300                                                                                                                                                                                                                                  GCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCACCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACC‑GGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGA                                                                                 <  2:375132/301‑1                                                                                                                                                                                                                                    AGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACC‑GGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATC                                                                               <  1:593280/301‑1                                                                                                                                                                                                                                     aAACCAGCACCAGCAAGGGGAACAAGGCCAGTCACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACC‑GGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCA                                                                              <  1:571794/300‑1                                                                                                                                                                                                                                            CACCAGCAAGGGGAACACGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGTTCCGCTTTGCGGCCCCAACTACAAACACCAAAGCCAATACAATCATCAGGTCTTCCGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCTTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCCCGCTGGTCAGTTCGACGTTGGAGTCCTCCAGGCCACCACC‑GCAAATCTGCCCGTGCGTACCGGCCTCCGTAGCGCCGTTCACCGGCAC                                                                       >  2:543902/1‑301                                                                                                                                                                                                                                                 GCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACC‑GGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGGCACGCCCG                                                                  <  1:123057/301‑1                                                                                                                                                                                                                                                   AAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCA‑CACCGGGAAATCTGGCCGAGGGTACCGTTGCCCGGAGCGCTGATCACCGGCACGCGCGAG                                                                >  2:596588/1‑301                                                                                                                                                                                                                                                                        GACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGCAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACC‑GGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGGCACGCCCGAGGCATAGG‑CTGCTTTCCAGTTG                                          >  2:491977/1‑301                                                                                                                                                                                                                                                                               GCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACC‑GGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGGCACGCCCGAGGCATAGGACT‑CTTTCCAGTTGGTACCGC                                   >  1:481492/1‑301                                                                                                                                                                                                                                                                                 TTCATTCGCGTTAACCTTCACACTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCGGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACC‑GGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGGCACGCCCGAGGCATAGGACT‑CTTTCCAGTTGGTACCGCCA                                 <  2:838347/301‑1                                                                                                                                                                                                                                                                                 TTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACC‑GGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGGCACGCCCGAGGCATAGGACT‑CTTTCCAGTTGGTACCGCCc                                 >  2:539513/1‑300                                                                                                                                                                                                                                                                                                               GCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACC‑GGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGGCACGCCCGAGGCATAGGACT‑CTTTCCAGTTGGTACCGCCAATCGGCGTCAGGCCCTGCAGGTTGGCGAAG   >  1:595153/1‑301                                                                                                                                                                                                                                                                                                                CCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTCATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACC‑GGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGGCACGCCCGAGGCATAGGACT‑CTTTCCAGTTGGTACCGCCAATCGGCGTCAGGCCCTGCAGGTTGGCGAAGT  >  2:346077/1‑301                                                                                                                                                                                                                                                                                                              |||                                                                                                                                                                                                                                                                                                               CCGGTTTACGGGGCTTTCAGCGCGGCAAGGGGCAATAACCGT‑CAGTTTGTCGGCGGATGTTTGCCGCCTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/4980286‑4980585 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACC‑GGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGGCACGCCCGAGGCATAGGACT‑CTTTCCAGTTGGTACCGCCAATCGGCGTCAGGCCCTGCAGGTTGGCGAAGT  >  AE015451/4980586‑4980885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.