| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 196,432 | 0 | T | C | 35.3% | 79.3 / 69.0 | 89 | T646T (ACT→ACC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base T (36/19); new base C (18/12); total (55/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGTCACCATCGAGAACGCCACGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGA‑TCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑GATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGT > AE015451/196137‑196726 | gCGTCACCATCAAGAACGCCACGGGCGGTAATTTCGAGCAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGA‑TCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAg < 1:356186/301‑1 (MQ=255) cGTCACCATCGAGAACGCCACGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAAGCGGCG‑GAGTCCCCTTCTCCACTACAGTCACCCAACCTGACCACACATC‑GCTGACACTGACCCTGTCAAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAAACCCCGGCGCATGACCTCTACAACCACGGTTCGC‑CTGTCAgt > 1:613184/1‑301 (MQ=14) caccatacagaACGCCACGGGCGGTAATTACTAGCAACTGACACCGTACCCGACTCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGA‑TCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CCGTCAGCGTc < 1:246434/293‑1 (MQ=11) aacATCGAGAACGACACGGGCGGTAATTGCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACGACTACCGCGTCCCTGACGGCGAGCCCGTCGGTGACCGAAGGCGGCGGGA‑TCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCa < 1:435716/299‑1 (MQ=255) aCCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGA‑TCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CCGTCAGCGTCa < 1:99954/301‑1 (MQ=12) gaACGCCACGGGCGGTAATTTCGAGCAACTGACCCCGGACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGA‑TCACGTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTAACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCgag < 1:237042/301‑1 (MQ=255) atcccccggcgggaaattccggacAACCGACCCCGCACCCGCCGCCGGCTCAACCCACAATCAACCACCCGGTCCAAACCCCTCCCGCGACCCTGACGGCGAGCCGGTCGTTCACCAAAGGCGGCTTGA‑TCATTTACCCCTTGCCCCGGACCACTCCGGCCCAGACGCCGGGTAACTG‑GACCTCGGCCACGGGCCAAACCATT‑CCCTTGAAGCCCGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCGAGAAc < 1:786275/278‑1 (MQ=2) cGCCACGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAA‑TCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTTACCGTGACCCTGTCCAACGGCCAAGCCATTACCGGTGAAGCCGGCAAGACCCAGGGCAGCGCCGATTTCCAGAACCCGGCCAATGACGTCTACAAAACCGGTTCGA‑CCGTCacaccacccatcccgaaa > 2:816488/1‑284 (MQ=11) gagttcagacgtgtgctcttccgatctccccGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGA‑TCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CCGTCAGCGTCACCATCGAGAACGc < 1:645433/274‑1 (MQ=11) ccACGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGA‑TCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CCGTCAGCGTCCCCATCGAGAACGc > 1:851629/1‑301 (MQ=16) cGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGA‑TCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGAACCCGGCGAATGACGTCTACAACAACGGTTCGACCTG‑CAGCGTCACCAGCGCGAACGcacc > 2:371189/1‑298 (MQ=11) gggCGGTAATTTCAAGCAACTGACCCCGCACCCGACGCCGGCTCAAACCACGATCAACGGCTCGGGCGACACCACCACCGCGACCCTGACGGCGAGCCCGTACGTCACCGAAGGCGGCGTGA‑TCACTTACACCGTGACACTGAGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGACAACACACAGGGCAGCGTCGATTTACAGACCCCGGCGAGTGACGTCTACAACAACGGTTCGA‑CCGTCAGCGTCACCATCGAGAACGCCAca < 1:661265/301‑2 (MQ=11) taagttctaGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGA‑TCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGt < 1:383593/293‑1 (MQ=255) tAATTTCGAGCAACTGACCCCGAATCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGA‑TCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGCGTCACCATCGAGAACGCCACtggcggc > 1:456463/1‑300 (MQ=18) atgtCGAGCAACTGACCCCGAACCCGACGACGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGA‑TCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CCGTCGGCGTCACCATCGAGAACGCCACAGGCGGTaa < 1:222636/298‑1 (MQ=255) atctaaCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGA‑TCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCg < 1:493585/297‑1 (MQ=255) gCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGATGGCGAGTCCGTCGGTCACCGAAGGCGGCGTGA‑TCACTTACACCCTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAg < 1:507719/301‑1 (MQ=255) acagcTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGA‑TCACCTACACCTTGAACGTGCGCACCTCTGCACAGAAGCC‑GGCGGCAGTGACCTTGTCCAACGGCCACTGGCTGACCGTTGAAGCCGGCTAAACTCACGGCAACGTCGATTTCCAGAACCCGCCGAATCACGCCCACAACAACGTCTCGA‑CCGTCAGTGTCACCATGTAGACCGCCAcctcccgtaatctcgag > 1:438013/5‑286 (MQ=2) aaCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGA‑TCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAgat > 2:493418/1‑299 (MQ=255) aCCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGA‑TCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACAGCCAAACAATTCCGGATGCAGCCGACAAGACCCAGGCCCGCGTCGCTTTCCATACCCGGGCGAATAACGTCGACAACAACGGTTCGA‑CTTGCAGTGTCACCACCGAAAACTCCACCGGCGGTAATTTCGAGCAACTGa > 1:541012/1‑301 (MQ=14) ccccGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGA‑TCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CCGTCAGCGTCACCATCGAGaaagcagatcggaagagcgtcgtgtagggaaa > 2:645210/1‑271 (MQ=11) gAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTTGGTCACCGAAGGCGGCGTGA‑TCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCg < 1:83844/301‑1 (MQ=255) aaCCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGA‑TCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CAGTCAGTGTCACCACCGAGAACGCCACCGGCGGCAACTTCGaccgacagagcgctg > 2:742847/1‑287 (MQ=255) cccGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGA‑TCACTTACACCGTGACCCTGTGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAAt < 1:383004/301‑1 (MQ=255) cccGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGA‑TCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTGACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCCGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCCATGTCACACTCCAGCAAGCCACCGGCGGTAATTTAGCGCAACTGACCCCGaaa > 2:382879/1‑300 (MQ=14) gACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGA‑TCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGCTTCACCATCGAGAACGCCACTGGCGGAAACTTCGAACAGCTGCCCCCGaacccagaccggaagagcgccagggacggaa > 2:756057/1‑272 (MQ=11) gACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGGCACCGAAGGCGGCGTGA‑TCACGTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAATCATCACCGTTGAAGCCGGCAAAACCCAGGGCAGCGGCGATTTCCAGACCCCGGCAAATGACGTCTACAACAACGGTTACA‑CCGTCAGCGCCACCATCCAGAACGCCACCGGCCGCAACTTAGAACCACCTACCCCGAATCCGACCC‑CGGCTCAGACCAAGGTCAAcc > 2:384903/1‑300 (MQ=11) acacCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGA‑TCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGCGTCACCATCGAGAACGCCACCGGCGGCAATTTCGAACAACTGACCCCGAACCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGa > 1:874401/1‑301 (MQ=255) cGGCGAGCCCGTCGGTCACCGACGGAGGCGTGA‑TCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGAc < 1:197588/301‑1 (MQ=255) cgatctcggcgTGA‑TCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGGGACAGTGACCCTGTCCAACGGCCCAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCAcc < 1:724577/295‑1 (MQ=255) cGAAGGCGGCGTGA‑TCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCggccccc > 2:438837/1‑296 (MQ=38) cggcgTGA‑TCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGC‑CGGCTCAGACCACGATCAACCACTCGGTCGA‑TGCCACAACCCCCACCCTGACGGCGAGCCCGTCGCTCAccagatcc > 2:724324/1‑295 (MQ=255) gTGA‑TCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCC‑GGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGCGTCACCATCGAAAACGAAAAAGACGCGAATGTAGACCAACTAACCGCGGACACGACGT‑CGGCTCAGACCACGATCAAAGAATAGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑ggacgcg > 1:520885/1‑296 (MQ=11) gA‑TCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCGCGAACGCCACCGCCCGTAATTTCGAGCAACTGACCCCGAATCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑TGC‑GG‑C‑GT‑g > 1:439597/1‑301 (MQ=255) cTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CCGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAACTTCGAGCAAGTGAGCACGAACACGACGC‑CGGCTCAGAGCACCATCAACGACTCGGTCGA‑TGCGACCACCGCGACCCAGAAGACAAGCCCGTCGTTCACCCAA‑GGC‑GG‑C‑GT‑GACCAc > 1:477936/1‑301 (MQ=11) gTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑GATCACC‑TACACCg < 1:438982/301‑1 (MQ=38) tctacccTGCCCAGACGCC‑GGTAACAGTACCCCTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑CGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑GATCACC‑TACACCGTGACCCTGAg < 2:467107/296‑1 (MQ=11) cAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGCAATTTCGAACAACTGACCCCGAACCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑GATCACC‑Tacacactcacacagcgtcgtcatggcc > 2:38577/1‑278 (MQ=11) gACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGCATGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAGCAACTGACCCCGAACCCGCCGC‑CGGCTCAGACCACGATCAACGACTCGGTCCA‑TGCCCCCACCGCGACCCTGCCGGCCAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑CT‑GATCACC‑TACACCCTGTCCCTggcccctccttcccaa > 1:745324/1‑286 (MQ=11) gTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑GATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑Cg‑g > 1:290852/1‑301 (MQ=38) ggACAGTGACCCTGTACAAAGGCCAAACCATTACCCGTGAAGGCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGACACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑GATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑Gt < 1:759406/300‑1 (MQ=255) aCAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAACTTCGAGCAACTGACCCCGAACCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCCCGACCCTGACGGCGAGCCCGTCGGTCCCCGAAGGGC‑GT‑C‑‑T‑GGCCACC‑TACACCGTGAcccgtacccttccggcccagatcggacgag > 2:490881/1‑274 (MQ=11) aCAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAAGGACGCGTATGACAACGGGTCAC‑CCGTCAGTGTCACCAATGCGGTCGCCACCGGCGGAAATTTAGAGCAACTGACCCCGAACCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGGCCA‑TGCCACCACCGCGACCCTCACGCCGATACCGTGGGTCACCCAT‑GGCGGG‑C‑G‑‑GATCACC‑TCCCCCGCTACCCTGAGcgatgaagcccagacgaccgtgc > 1:557243/1‑279 (MQ=2) aCAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCAAGACCGCGGTGAATTACGTCTACAACAACGGGTCGA‑CTGTCAGTGTCACCATCGAAAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCTACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑GATCACC‑TACACCCTCACCCTTAGCAATCCTTCCCACAAGC‑CG‑Gtct > 1:777011/1‑299 (MQ=14) ccAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TACCACTACCGCGACCCTGACGACGAGCACACGGGTCAACGAC‑AGC‑CG‑C‑GT‑GATGACC‑TATCCCGTGACCCCTAGTAATCCTGCCAAAAAGC‑CG‑GTGCCAGTGACCCTGTc > 1:518510/1‑301 (MQ=11) cAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑GATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCTTGTcc < 1:808042/301‑1 (MQ=255) ccAAACGAGTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGACTACAACAACGGTTCGA‑GTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCC‑CGGCTTAGACTACTTTCTACTACGCCGTCCACGGAGACC‑TAGCCTCTTTCCCGGCACGACGCTCGTCCGCCCAA‑GGC‑GG‑C‑GT‑GATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGc < 2:223343/301‑1 (MQ=2) aaaCCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACACCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGGCGGTCACCGAA‑GGC‑GG‑C‑GT‑GATCACC‑TACACCGTGACCCTGAGCAATCCTAGCCAGAcagcggtaacacgtaccctgacctaactcaa > 1:561182/1‑271 (MQ=11) gtgccgtacaaGCAGGCAAAACCCAGGACAGCGTCGATTTTCTGCACGCGGAGGATGACATATACAACTACGTCACGA‑CACTCAGTGTCAGCAGCGAGAACGCCAGCGCTGGTAATTTCGAGCAACTGACCCCGAACCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑GATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCAt < 1:10918/292‑1 (MQ=11) gtggaGCCGGCAAAAACCATGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CCGTAAGCGTCACCATCGAGAACGCCACCAGCGGCAATTTCGAGCAACTGACCCCGAACACGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGAGGCCCTGACGGTGTGTTTGTCGGGCTCCGGC‑GGC‑GG‑C‑CTAGA‑CATC‑CACTCCGTCCCCCCGCGCCACCCTTCCGATATGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGt < 2:366824/297‑1 (MQ=2) ggCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑AATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACCGTGAACCTGTCCAACGGCCAAACCATTACCGTTGAAGCCg > 1:52656/1‑301 (MQ=11) aagaCCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑AATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTCCCGTTGAAGCCGGCa > 2:501770/4‑301 (MQ=255) aCCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAACAGACCC‑CGGCTGAGACCACGACAAACGACTCGGTCGA‑GGCCACCACCGCGACCCTGACGGAGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑CATCACT‑TACACCGTGACCCTGAGCAACCCTGCCCAGACGC‑CG‑GTGACCGTGACCCTGTCCAACGGCCACAACATTACCGTTGAAGCTGGCAAGa > 1:641912/1‑301 (MQ=11) aCCCAGCGCAGCGTCTATTTCCAGACCCCGGCGAATGACCTCTACAACAACGTTTCGA‑CCGACAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCCCCGCGACCCTGCCGGCGAGCCCGGCGGTCACCGAA‑GGC‑GG‑C‑GT‑AATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGa < 1:602762/301‑1 (MQ=11) cccAGGGCAGCGTCGATTTCCAGCCACCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACGGACCCCGAATCCGACGC‑CGGCTCAGACCATGATCAACGACTCGGTCGA‑TGCCACCGCCCCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑GATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGAc < 1:102774/301‑1 (MQ=255) cccgggCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CCGTCAGCGTCACCATCGAGAACGCCACAGTCGGTAATTTCGAGCAACTGACCCCGAATCCGACCC‑CGGCTCAGACCACGATCAACGTCTCGGTCGA‑TGCCACCACCGCGACCCCGACGGCTGGCCCGTCGGTAAACGAA‑GGC‑GG‑C‑GT‑GATCCCC‑TACACCGTGACCCTGAGCAACCCTGCCCAGACGC‑CG‑GTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAAcc < 1:293035/298‑1 (MQ=11) ggCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑AATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAgg < 1:501937/301‑1 (MQ=255) gTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑GATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCg > 1:347896/1‑301 (MQ=255) gTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑AATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCg > 1:741292/1‑301 (MQ=255) cGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑GATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTc > 1:267519/1‑301 (MQ=255) cGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGCGTCAACATCGAAAGCGCCACATGCAGTAATTTCGAGCAAATCACCCCGAACCCGATAC‑CGTCTAAAACCACAATCAACGACTCGGTCGA‑CACCACTACCGCCACCCTGACGGCGATCCCGCCGGTCACCGAA‑GTC‑GT‑C‑GT‑GAT‑ACCTTACATCGTGCCCCTGAGCCATCCCGCCCAGCCGC‑CG‑CTGACAATGCCCCTGTCCATCGACCCCACCATTACCATTTAAGCCgtcacgccaccgaccacagtc > 2:99924/1‑281 (MQ=2) aTTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑AATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGa > 1:860374/1‑301 (MQ=255) ccAGACGCCGGCGACTGACGTCTACACCAACGGTTCAA‑GCACCATCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACACCGAAGCCGACCC‑CGGCTCAGACCACGATCAACGACTCGTTCGA‑GGCCACCACCGCGACCCTGACGGCGAGCCCGTCTGTCATCGAA‑GGC‑CG‑C‑GG‑AACCACG‑TACACCACGCTCCTGCCCAACCCGGCGCACTC‑C‑CGAGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTc < 2:56455/301‑1 (MQ=2) aGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TACCACCACAGCGCACCTGACGGAAATCCCGTCGCTCACCGAA‑AGC‑GC‑G‑GA‑TATCACC‑TACAGCTTGACCCTGAACAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCCATTTCCa > 1:132649/1‑301 (MQ=11) ccGGCGAATGACGTCTACAACAACGGTTCGA‑CCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCC‑CGGCTCAGACCACGATCAACGAGTCGGTCGA‑TGCAACCACCGGGACCCTGACGGCGAGCCCGTAGGTCACCGAA‑GGC‑GT‑C‑GT‑ACTCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGCCAGCGTCGATTTCCAGAccc > 1:710669/1‑301 (MQ=11) cGGCGAATGTAGTCTACAAAAAAGGTTCGA‑CGGTCAGGGTCACCATCGAGAACGCCACAGGCGGTAAGTTCGAGCAACTGACCCCGAAGCCGACGC‑CGGCTCAGAGCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTTTGTCACCTTA‑AGC‑GGACGGC‑TAACACA‑GAGATCTACACTCTG‑TCCCTAC‑GCGCCG‑CTCTTC‑GTAACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGAcccc < 2:406739/301‑1 (MQ=2) agacgagtGACGTCTACAACAACGGTTCGA‑CCGTCAGCGTCACGATCGAGCAGGCCACAGGCGGTAAGTTCGAGCAACAGACCGCGAATCCGACCC‑CGGCTGAGACCACGATCAACGTCTCGGTCGA‑GGCCACCACCGCGACCGAGACTGCGACCACGGCCGTGAGCGAA‑GAC‑GG‑C‑GT‑AATCACC‑TACCCCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGAcccc < 1:638670/294‑1 (MQ=11) ggCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑GATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GGTACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGACGCCGGCAAGACCCacgacagagtcaacacgcctaccctc > 1:691898/1‑276 (MQ=14) gatctaTGACGTCTACAACACCGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑GATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCg < 1:443863/296‑1 (MQ=255) caaaTGACGTCTACAACAACGGTTCGA‑CCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑AATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGc > 1:599735/3‑301 (MQ=255) cGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑GATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGc > 2:230092/1‑301 (MQ=255) gAATGACGTCTACAACAACGGTTCGA‑CTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCC‑CGGCTCAGCCCACGATCAACGACTCGGTCGA‑CGCCACCACCCCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GC‑AATCACC‑TACACCGTGACCCTGAGCAACCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTCGAAGCCGGCAAGACCCAGGGAAGCgacgtcgtaagagcacgcgcc > 1:705985/1‑281 (MQ=11) gAATGACGTCTACAACAACGGTTCGA‑CTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑AATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCCGCGTCGATTTCCAGACCCCGGcc > 1:503518/1‑300 (MQ=255) aTGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑GATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGGCCAACGGCCAAACCATTACCGTTGAAGACGGCAAAACACAGGGCAGCGTCGATGTCCAGACCCCgagatt > 2:443709/1‑296 (MQ=255) tGACGTCTACAACAACGGTTCGA‑CCGTCAGCGTCCCCGTCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCC‑CGGCTCAGACCACGCTCACCGACTCGGTCGA‑TGCCCCCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑CGC‑GG‑C‑GT‑AATCACC‑TACACCGTGACCCTGAGCATTCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAGCCCATTACCGTTGAAGCCGGCAAGACCCATGGCAGCGTCGATTTCCAGACCCCCGCGAAt > 1:40535/1‑301 (MQ=11) cactttttccactacGGTTTGA‑CTGGCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGC‑CGGCTCAGACCCCGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCTAA‑GGC‑GG‑C‑GT‑GATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAGGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATg < 2:23827/288‑1 (MQ=14) cTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑GATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTTACCCTGTCCAACGGCCAAAGCATCACAGATGAAGCCGCCCAGACCCAGGCCACCGTCGATTTCCAGACTCCCGCCATTGACGTc > 1:678371/1‑301 (MQ=14) aacaacaACGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GT‑C‑GT‑TATCACC‑TACACCGTGACCCTGCGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCt < 1:312678/300‑1 (MQ=255) aacaacGGTTCGA‑CTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑AATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTaca > 1:514752/1‑301 (MQ=255) acaacGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑GATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGTGCAGCGTCGATTTCCAGACCCCGGCGAATCACGTCTacaa > 1:825988/1‑301 (MQ=255) acaacGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑GATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGTCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTacaa > 1:514066/1‑301 (MQ=255) acaacGGTTCGA‑CCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑AATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTacaa > 1:504110/1‑301 (MQ=255) caacGGTTCGA‑CCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCC‑CGGCTCAGACCACGATCAACGACTCGGACGA‑TCCCACCTCCGCAATCCTGACACCCAGCGCGTCGTCCTCTGCC‑GTC‑GT‑C‑GT‑CATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAACGACGTCTacaac > 1:723336/1‑301 (MQ=11) aacGGTTCGA‑CTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCCACCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑AATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTacaaca > 1:663370/1‑301 (MQ=255) cGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑GATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATCACCGTTGAAGCCGGCAAGACCCAGGGCAGCGACGAGTTCCAGACCCCGGCGAATGagatcggaagggca > 1:23833/1‑288 (MQ=255) cGGTTCGA‑CCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATGCGA‑GCACGGCTCAGAGCACGATCAACGATTCGGACGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑AATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑AG‑GTGACAGTAACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACACCGGCGAATGACGTCTACaaaaac > 2:161410/1‑301 (MQ=11) ggTTCGA‑CCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑AATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACg > 1:770683/1‑301 (MQ=255) gTTCGA‑CGGTCAGCGTCACCATCGAGCACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑CGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑GATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACgg < 2:113054/301‑1 (MQ=9) ttCGA‑CTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑AATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACggg > 1:382960/1‑300 (MQ=255) | GCGTCACCATCGAGAACGCCACGGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGA‑TCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCC‑GGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGA‑CTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACGC‑CGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAA‑GGC‑GG‑C‑GT‑GATCACC‑TACACCGTGACCCTGAGCAATCCTGCCCAGACGC‑CG‑GTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGT > AE015451/196137‑196726 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |