Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451 1,499,480 2 bp→AC intergenic (+57/‑106) trpS → / → zapE Tryptophan‑‑tRNA ligase/putative enzyme with nucleoside triphosphate hydrolase domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154511,499,4800CA93.3% 93.7 / NA 30intergenic (+57/‑107)trpS/zapETryptophan‑‑tRNA ligase/putative enzyme with nucleoside triphosphate hydrolase domain
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (12/16);  total (14/16)
Fisher's exact test for biased strand distribution p-value = 2.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*AE0154511,499,4810AC96.7% 93.8 / NA 30intergenic (+58/‑106)trpS/zapETryptophan‑‑tRNA ligase/putative enzyme with nucleoside triphosphate hydrolase domain
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (13/16);  total (14/16)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGGCCGCTGATGGCGAGCAACTGCTGTTGTCGCGCAGCTTCGCCGACGGCAAGAGTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑TCGGGCACG‑C‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAA‑CA‑ACAAGCCGGGCAT‑GTTCGGCAAGCTGTTCGGCAAGAAGGAGCAGACTCCGGTCAAGGG  >  AE015451/1499183‑1499768
                                                                                                                                                                                                                                                                                                         ||                                                                                                                                                                                                                                                                                                      
gctgGCCGCTGATGGCGAGCAACTGCTGTTGTCGCGCAGCTTCGCCGACGGCAAGAGTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTCGCACCACAGCAGGACTGAGGCTGCCAAACGGTCGGAGAGGGCGTGCCCGCGAAACAGGCGCCGAGGTGCCTGGccccc                                                                                                                                                                                                                                                                                                      >  2:454247/1‑298 (MQ=255)
         tGATGGCGAGCAACTGCTGTTGTCGCGCAGCTTCGCCGACGGCAAGAGTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCg                                                                                                                                                                                                                                                                                             >  1:436110/1‑301 (MQ=255)
             ggCGAGCAACTGCTGTTGTCGCGCAGCTTCGCCGACGGCAAGGGTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGgtgt                                                                                                                                                                                                                                                                                         <  1:160276/301‑1 (MQ=255)
               cGAGCAACTGCTGTTGTCGCGCAGCTTCGCCGACGGCAAGAGTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAAGGTGGGGGCGGGCGTGCCCGCGAAACAGGCGCGCCGGGGCAGGGCCACCGCtgtggcgggcgtg                                                                                                                                                                                                                                                                                       >  2:208396/1‑289 (MQ=255)
                gAGCAACTGCTGTTGTCGCGCAGCTTCGCCGACGGCAAGAGTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACCGGCGCCGTCGTGCATCGCCCCGGCTTTCGCGGTGt‑tcc                                                                                                                                                                                                                                                                                     >  2:93304/1‑299 (MQ=255)
                 aGCAACTGCTGTTGTCGCGCAGCTTCGCCGACGGCAAGAGTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTTTGGCTCAATGGCGAGCAGGTTGCCGCCTGGCCGCAGTTCGACAACGCTGCAGCCCGTGCTGCGGCCATTCAGAGCCTGAACGAAGCCTTTGCACCACAGCAGGCCTGCGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGAGACACAAGCCCCTCGGTGCATGGCACCGGATTTGCCGGGGt‑tcgc                                                                                                                                                                                                                                                                                    >  2:644308/1‑298 (MQ=255)
                          tgttgtCGCGCCGCTTCGCCGACGGCAAGAGTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCAcg‑c‑c                                                                                                                                                                                                                                                                         <  1:208465/301‑1 (MQ=255)
                                        ctttcccgacacgacgcctttccgatctggAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACt                                                                                                                                                                                                                                                            <  2:98734/272‑1 (MQ=255)
                                            ccGACGGCAAGAGTGCTGGCGACGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGACGATGTCCGCATCGAAGGCCTGAGCGTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGAtt                                                                                                                                                                                                                                                        <  1:180033/301‑1 (MQ=255)
                                                                    tGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACtagatcggaagagcacacgtctgaactcc                                                                                                                                                                                                                                >  1:98764/1‑273 (MQ=255)
                                                                                                         ccGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTAAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATc                                                                                                                                                                                           <  1:37737/301‑1 (MQ=255)
                                                                                                           ggagcGAAGGGCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGAAAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt                                                                                                                                                                                         <  2:757718/297‑1 (MQ=255)
                                                                                                                     catgggCTTCGGCCTGTGGCTAAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa                                                                                                                                                                               <  2:435965/296‑1 (MQ=255)
                                                                                                                            ttgtgCCTGTTGCTGAAGGGCGCGCAGGTTGCGGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCCCAGCAGGACTGAGGCTGCCAAACGGTGGGTGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCt                                                                                                                                                                        <  2:274237/297‑1 (MQ=255)
                                                                                                                                   tgtgGCTAAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGc                                                                                                                                                                 <  1:93334/301‑1 (MQ=255)
                                                                                                                                     tgGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACCGACTGATTGCCATTAAGCGTGCCTATCGCTACAGTGACGGCTCGTTTTTTGTTGCCTAGCTCACGAATCATCACACCCTTCGAACGCTATCAAGCac                                                                                                                                                               >  2:536044/1‑300 (MQ=255)
                                                                                                                                                             gACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCCTAGAACGCTAACAAGCAAGTCTGAAACGTCCCgtacttttcc                                                                                                                                       >  1:834692/1‑292 (MQ=255)
                                                                                                                                                                                 cGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACt                                                                                                                   <  1:454401/301‑1 (MQ=255)
                                                                                                                                                                                       agaccgagatggggcaatgcaaggCCTGAAAGAAGACTTGGCAACAAAGCAGGAATGAGGCTGCAAAACGGTGGGGACGGGCGTGCCCGAGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTg                                                                                                             <  2:677825/278‑1 (MQ=255)
                                                                                                                                                                                        ccccGTGATGCGGCCATTCAGAGCCGGAACGAAGCCTTGGCACCACAGCCGGACTGAGGCTGCCAAACGGTGGGGGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGc                                                                                                            <  2:68285/300‑1 (MQ=255)
                                                                                                                                                                                                               ccTGAAGGAAGCCTTGGCCACACAGCAGGGCTGAAGCTGGCAAACGGCTGGAGCGGGCGGGCCCGCGAACGCGGCGCCGCGCGGCATGGCACCGGCGTGGCCGGTGT‑TCACGGCCACG‑CGCGCTCCCACAGACTGATTGCTATTAAGCGGGGCTATCGCTACAGTGACCGGCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAAGGCTATCAAAAAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGGCACCTCCAGCGCCTGTacgac                                                                                     <  2:665376/301‑1 (MQ=255)
                                                                                                                                                                                                               ccTGAACGATGCCTTGGCCCCAACGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTacgac                                                                                     <  1:866569/301‑1 (MQ=255)
                                                                                                                                                                                                                                      agcagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGAGGGCACGCC‑CGCTCCCACAGATTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTACCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGAATTCTTCCATTACTCTGCGCAGGAAACTGCGGTACGTCCCCTCCAGCGCCTGTACCACGACCTTGTGCACGCGCAGaa‑ca‑a                                                            >  1:53790/1‑301 (MQ=255)
                                                                                                                                                                                                                                                   ctggcAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAATGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAA‑CA‑ACAAGCCGGGCAT‑g                                              <  1:99000/297‑1 (MQ=255)
                                                                                                                                                                                                                                                        aaaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAAATGCGGTGCGTCACCCCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACCA‑AC‑AGCCGGGCAT‑GTTCgg                                         >  2:405514/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                         gTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCTGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATCACGCCGCGCAGGAAACTGCGGTCGGTCACCTCCAACGCCTGTACGACCACCTGGTGCACGCGCAGAA‑CA‑ACAAGCCGGGCGT‑GTTCGGCAAGCTGTTCGGCaaga                        >  1:563097/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                         gTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCCCCTCCAGCGCCTGTACGGCGGCCTGGGTCACGCGCAGAA‑CA‑AAAAGCCGGGCCT‑GTTGCGCAACCTGttggcgaaga                        >  2:750702/1‑293 (MQ=255)
                                                                                                                                                                                                                                                                           gCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCCCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTCACGAATCATGACCCCCTTAGAACGCTATCAAGCAGATCTGACACGTCCCGACTCCTTCCATGACGCGGCCCAGGAACCTTCGGTGCGTCACCTCCAGCGCCTGTACGCCCACCTGGTGCACGCGCAGAA‑CA‑ACAAGCCGGGCaaagagcggaagagcgcacttctcaac                       >  1:447933/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                                          cccgcGGTGCATGGCACCGGCTTTGCCGGTGTGC‑GCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAA‑CA‑ACAAGCCGGGCAT‑GTTCGGCAAGCTGTTCGGCAAGAAGGAGCAGACTCCGGTc       <  1:644531/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                            cgcgGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCCGCGCCTGTACCACGGCCTGGTGCCCCCGCCG‑A‑CACACAAGCCGGGCATGGTGCGGCAGGCTGtccgccacgaggcggcaaccccggtcaa      >  2:557737/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                                               ggTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTTCACGCGCAGAA‑CA‑ACAAGCCGGGCAT‑GTTCGGCAAGCTGTTCGGCAAGAAGGAGCAGACCCCGGTCAAggg  >  2:515511/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                         ||                                                                                                                                                                                                                                                                                                      
GCTGGCCGCTGATGGCGAGCAACTGCTGTTGTCGCGCAGCTTCGCCGACGGCAAGAGTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑TCGGGCACG‑C‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAA‑CA‑ACAAGCCGGGCAT‑GTTCGGCAAGCTGTTCGGCAAGAAGGAGCAGACTCCGGTCAAGGG  >  AE015451/1499183‑1499768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑