Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451 1,499,498 1 bp→CG intergenic (+75/‑89) trpS → / → zapE Tryptophan‑‑tRNA ligase/putative enzyme with nucleoside triphosphate hydrolase domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154511,499,4971.C96.3% 88.7 / ‑3.3 27intergenic (+74/‑90)trpS/zapETryptophan‑‑tRNA ligase/putative enzyme with nucleoside triphosphate hydrolase domain
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (10/16);  total (10/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01
*AE0154511,499,4980TG96.3% 86.7 / NA 27intergenic (+75/‑89)trpS/zapETryptophan‑‑tRNA ligase/putative enzyme with nucleoside triphosphate hydrolase domain
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (10/16);  minor base A (0/1);  total (10/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

TGTTGTCGCGCAGCTTCGCCGACGGCAAGAGTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑TCGGGCACG‑C‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAA‑CA‑ACAAGCCGGGCAT‑GTTCGGCAAGCTGTTCGGCAAGAAGGAGCAGACTCCGGTCAAGGGCCTGTACTTCTGGGGTGGGGTGGGGCGAGG  >  AE015451/1499209‑1499798
                                                                                                                                                                                                                                                                                                   ||                                                                                                                                                                                                                                                                                                                
tgttgtCGCGCCGCTTCGCCGACGGCAAGAGTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCAcg‑c‑c                                                                                                                                                                                                                                                                                                       <  1:208465/301‑1 (MQ=255)
              ctttcccgacacgacgcctttccgatctggAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACt                                                                                                                                                                                                                                                                                          <  2:98734/272‑1 (MQ=255)
                  ccGACGGCAAGAGTGCTGGCGACGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGACGATGTCCGCATCGAAGGCCTGAGCGTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGAtt                                                                                                                                                                                                                                                                                      <  1:180033/301‑1 (MQ=255)
                                          tGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACtagatcggaagagcacacgtctgaactcc                                                                                                                                                                                                                                                              >  1:98764/1‑273 (MQ=255)
                                                                               ccGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTAAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATc                                                                                                                                                                                                                         <  1:37737/301‑1 (MQ=255)
                                                                                 ggagcGAAGGGCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGAAAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt                                                                                                                                                                                                                       <  2:757718/297‑1 (MQ=255)
                                                                                           catgggCTTCGGCCTGTGGCTAAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa                                                                                                                                                                                                             <  2:435965/296‑1 (MQ=255)
                                                                                                  ttgtgCCTGTTGCTGAAGGGCGCGCAGGTTGCGGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCCCAGCAGGACTGAGGCTGCCAAACGGTGGGTGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCt                                                                                                                                                                                                      <  2:274237/297‑1 (MQ=255)
                                                                                                         tgtgGCTAAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGc                                                                                                                                                                                               <  1:93334/301‑1 (MQ=255)
                                                                                                           tgGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACCGACTGATTGCCATTAAGCGTGCCTATCGCTACAGTGACGGCTCGTTTTTTGTTGCCTAGCTCACGAATCATCACACCCTTCGAACGCTATCAAGCac                                                                                                                                                                                             >  2:536044/1‑300 (MQ=255)
                                                                                                                                   gACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCCTAGAACGCTAACAAGCAAGTCTGAAACGTCCCgtacttttcc                                                                                                                                                                     >  1:834692/1‑292 (MQ=255)
                                                                                                                                                       cGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACt                                                                                                                                                 <  1:454401/301‑1 (MQ=255)
                                                                                                                                                             agaccgagatggggcaatgcaaggCCTGAAAGAAGACTTGGCAACAAAGCAGGAATGAGGCTGCAAAACGGTGGGGACGGGCGTGCCCGAGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTg                                                                                                                                           <  2:677825/278‑1 (MQ=255)
                                                                                                                                                              ccccGTGATGCGGCCATTCAGAGCCGGAACGAAGCCTTGGCACCACAGCCGGACTGAGGCTGCCAAACGGTGGGGGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGc                                                                                                                                          <  2:68285/300‑1 (MQ=255)
                                                                                                                                                                                     ccTGAAGGAAGCCTTGGCCACACAGCAGGGCTGAAGCTGGCAAACGGCTGGAGCGGGCGGGCCCGCGAACGCGGCGCCGCGCGGCATGGCACCGGCGTGGCCGGTGT‑TCACGGCCACG‑CGCGCTCCCACAGACTGATTGCTATTAAGCGGGGCTATCGCTACAGTGACCGGCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAAGGCTATCAAAAAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGGCACCTCCAGCGCCTGTacgac                                                                                                                   <  2:665376/301‑1 (MQ=255)
                                                                                                                                                                                     ccTGAACGATGCCTTGGCCCCAACGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTacgac                                                                                                                   <  1:866569/301‑1 (MQ=255)
                                                                                                                                                                                                            agcagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGAGGGCACGCC‑CGCTCCCACAGATTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTACCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGAATTCTTCCATTACTCTGCGCAGGAAACTGCGGTACGTCCCCTCCAGCGCCTGTACCACGACCTTGTGCACGCGCAGaa‑ca‑a                                                                                          >  1:53790/1‑301 (MQ=255)
                                                                                                                                                                                                                         ctggcAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAATGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAA‑CA‑ACAAGCCGGGCAT‑g                                                                            <  1:99000/297‑1 (MQ=255)
                                                                                                                                                                                                                              aaaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAAATGCGGTGCGTCACCCCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACCA‑AC‑AGCCGGGCAT‑GTTCgg                                                                       >  2:405514/1‑301 (MQ=255)
                                                                                                                                                                                                                                               gTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCTGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATCACGCCGCGCAGGAAACTGCGGTCGGTCACCTCCAACGCCTGTACGACCACCTGGTGCACGCGCAGAA‑CA‑ACAAGCCGGGCGT‑GTTCGGCAAGCTGTTCGGCaaga                                                      >  1:563097/1‑301 (MQ=255)
                                                                                                                                                                                                                                               gTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCCCCTCCAGCGCCTGTACGGCGGCCTGGGTCACGCGCAGAA‑CA‑AAAAGCCGGGCCT‑GTTGCGCAACCTGttggcgaaga                                                      >  2:750702/1‑293 (MQ=255)
                                                                                                                                                                                                                                                 gCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCCCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTCACGAATCATGACCCCCTTAGAACGCTATCAAGCAGATCTGACACGTCCCGACTCCTTCCATGACGCGGCCCAGGAACCTTCGGTGCGTCACCTCCAGCGCCTGTACGCCCACCTGGTGCACGCGCAGAA‑CA‑ACAAGCCGGGCaaagagcggaagagcgcacttctcaac                                                     >  1:447933/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                cccgcGGTGCATGGCACCGGCTTTGCCGGTGTGC‑GCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAA‑CA‑ACAAGCCGGGCAT‑GTTCGGCAAGCTGTTCGGCAAGAAGGAGCAGACTCCGGTc                                     <  1:644531/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                  cgcgGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCCGCGCCTGTACCACGGCCTGGTGCCCCCGCCG‑A‑CACACAAGCCGGGCATGGTGCGGCAGGCTGtccgccacgaggcggcaaccccggtcaa                                    >  2:557737/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                     ggTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTTCACGCGCAGAA‑CA‑ACAAGCCGGGCAT‑GTTCGGCAAGCTGTTCGGCAAGAAGGAGCAGACCCCGGTCAAggg                                >  2:515511/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                ccGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACATACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAA‑CA‑ACAAGCCGGGCAT‑GTTCGGCAAGCTGTTCGGCAAGAAGGAGCAGACTCCGGTCAAGGGCCTGTACTTCt                     <  1:507668/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                ccGGCTTTGCCGGTGT‑TCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAA‑CA‑ACAAGCCGGGCAT‑GTTCGGCAAGCTGTTCGGCAAGAAGGAGCAGACTCCGGTCAAGGGCCTGTACTTCt                     <  1:405649/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                    cGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAA‑CA‑ACAAGCCGGGCAT‑GTTCGGCAAGCTGTTCGGCAAGAAGGAGCAGACTCCGGTCAAGGGCCTGTACTTCTGGGGTGGGGTGGGGCGAgg  >  1:73875/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                   ||                                                                                                                                                                                                                                                                                                                
TGTTGTCGCGCAGCTTCGCCGACGGCAAGAGTGCTGGCGCCGTGAGCAAGCAGCTGCAGCAAGGTGGCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑TCGGGCACG‑C‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAA‑CA‑ACAAGCCGGGCAT‑GTTCGGCAAGCTGTTCGGCAAGAAGGAGCAGACTCCGGTCAAGGGCCTGTACTTCTGGGGTGGGGTGGGGCGAGG  >  AE015451/1499209‑1499798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑