Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451 4,586,030 2 bp→TC intergenic (+113/+101) PP_4061 → / ← PP_4063 conserved protein of unknown function/putative Long‑chain‑fatty‑acid‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154514,586,0300CT92.3% 32.4 / ‑4.5 13intergenic (+113/+102)PP_4061/PP_4063conserved protein of unknown function/putative Long‑chain‑fatty‑acid‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (5/7);  total (6/7)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01
*AE0154514,586,0310TC92.3% 34.9 / NA 13intergenic (+114/+101)PP_4061/PP_4063conserved protein of unknown function/putative Long‑chain‑fatty‑acid‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (5/7);  minor base G (1/0);  total (6/7)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGAGGTCAGGCCATGAACATTCCCATTCCACCGGAGACACCCGATCCCAATATCGACGACCCGAGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TCTGC‑GGTAAACCCGCTCCTACCAGGG‑A‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTGCGATGCGTGCCTTGCACCA  >  AE015451/4585743‑4586278
                                                                                                                                                                                                                                                                                                ||                                                                                                                                                                                                                                                          
gcgcggTCAGGCCATGAACATTCCCATTCCACCGGAGACACCCGATCCCAAGATCGACGACCCTGGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAAccc                                                                                                                                                                                                                                                <  2:698205/297‑1 (MQ=255)
         ccccATGAAAATTCCCACTACACCGGAGACCCCCGACCACAATATCGACGACCCGACCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTAcc                                                                                                                                                                                                                                       <  2:372502/299‑1 (MQ=255)
           ccATGAACATTCCCATTCCACCGGAGACACCCGATCCCAATATCGACGACCCGAGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGGG‑GGTAAACCCGCTCCTACCac                                                                                                                                                                                                                                     >  2:34956/1‑300 (MQ=255)
                  cattcccactccACCGGAGACACCCGATCCCAATAGCGACGACCTGAGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGCGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGG‑A‑ccgt                                                                                                                                                                                                                            <  2:447741/301‑4 (MQ=255)
                                                 aaTATCGACGACCCGAGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGTCCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGACGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGCC‑CGGACCGGCCTTGAATGAGGCGTTGCACCTGGtc                                                                                                                                                                                              >  2:205962/1‑300 (MQ=255)
                                                        tcgcacctaGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGACGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCgg                                                                                                                                                                                       <  1:19172/293‑1 (MQ=255)
                                                                aGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGACGGCTTAAGGGTGGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGACGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTACACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTTCATGCGGCGCTGCCCTCGGTAGGacgggatctcggg                                                                                                                                                                               >  2:19170/1‑289 (MQ=255)
                                                                                                     aGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTCCCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGTCAGGAG‑GGGTGTACCCGCGAAGTGGCCGGCCGCGCCAGCGCATCAACCCGCAGa                                                                                                                                         >  1:659086/1‑301 (MQ=255)
                                                                                                                                                                     gAAGGCTTAAGGGTAGACCACGGCACCGGAGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGCTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCcggtcac                                                                          <  1:34962/301‑1 (MQ=255)
                                                                                                                                                                                ggTAGACCACGGCAACGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGAGCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGt                                                               <  1:206031/301‑1 (MQ=255)
                                                                                                                                                                                                         aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGTGCATCGGGTACTCGGCGACAAAACGTATATAccc                                      >  2:199875/1‑300 (MQ=255)
                                                                                                                                                                                                                      tgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCAGGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTAGACCAAACGTATATACCACGGCACCTTGacg                         >  2:733735/1‑299 (MQ=255)
                                                                                                                                                                                                                                             gACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTGCGATGCGTGCCTTGCACCa  <  1:199944/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                ||                                                                                                                                                                                                                                                          
GCGAGGTCAGGCCATGAACATTCCCATTCCACCGGAGACACCCGATCCCAATATCGACGACCCGAGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TCTGC‑GGTAAACCCGCTCCTACCAGGG‑A‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTGCGATGCGTGCCTTGCACCA  >  AE015451/4585743‑4586278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑