Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451	3,137,784	(T)9→8	intergenic (+212/‑78)	PP_2753 → / → PP_2754	putative Branched‑chain amino acid ABC transporter, ATP‑binding protein/putative OprD family outer membrane porin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	3,137,776	0	T	.	100.0%	176.9 / NA	39	intergenic (+204/‑86)	PP_2753/PP_2754	putative Branched‑chain amino acid ABC transporter, ATP‑binding protein/putative OprD family outer membrane porin
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (19/20);  total (19/20)

TCCTGGAAAACGGCCGGGTGGTAGGTGAGGGCAGTGCTGCCGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGTTTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAATTTCTTCTTCAGCCAGGACTATCGCAACGGCGACTTTACACTCAATCCCAATAATGGCGAGCGGCAGAGCCTGCATCGTGAATG  >  AE015451/3137478‑3138070                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                       tccgggAAAACGGCCGGGTGGTAGGTGAGGGCAGTGCTGCCGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGttt                                                                                                                                                                                                                                                                                                      <  2:724215/297‑1 (MQ=255)   cTGGAAAACGGCCGGGTGGTAGGTGAGGGCAGTGCTGCCGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATCGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGttttt                                                                                                                                                                                                                                                                                                    >  2:83485/1‑301 (MQ=255)               cGGGTGGTAGGTGAGGGCAGTGCTGCCGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCCGCCCAGGCCGCGTTTTTTTCGCCCTGACGGAAGCGCTGTGGCGCCGGCAACAGCAGCTGTGCAGATTGCTTCGCAGACGGCGCATCAGAGGGGTGAGGAAGTTGTTCAATCGCTTGAATAACAAAGG‑TTTTTTTGGTTGGCATg                                                                                                                                                                                                                                                                                       >  2:199360/1‑301 (MQ=255)                gggTGGTAGGTGAGGGCAGTGCTGCCGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATgg                                                                                                                                                                                                                                                                                      <  1:248878/301‑1 (MQ=255)                     gtAGGTGAGGGCAGTGCTGCCGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGgttgtt                                                                                                                                                                                                                                                                                 <  2:349413/301‑1 (MQ=255)                                 aGTGCTGCCGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGTCATGGTTGTTGGAACAACGGGa                                                                                                                                                                                                                                                                     >  2:725616/1‑301 (MQ=255)                                       gcCGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCa                                                                                                                                                                                                                                                               <  1:70502/301‑1 (MQ=255)                                           aGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCCTAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAgcgc                                                                                                                                                                                                                                                           >  1:239740/1‑301 (MQ=255)                                                                               ggggCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCAc                                                                                                                                                                                                                       <  2:239650/300‑1 (MQ=255)                                                                                      cAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCACCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCAtt                                                                                                                                                                                                                >  2:583972/1‑300 (MQ=255)                                                                                                        tGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCCTTCCAAGGATCACTGCCATTCGCTCTACCGTTTGCTAc                                                                                                                                                                                              >  2:109485/1‑301 (MQ=255)                                                                                                            gACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGGTGAGGAAGTTGTTCAATTGCTTGAATGACAATGG‑TTTTTTTTGTTGGCAGGGTTGGTGGAACCACGGGATCTGCAGCGCCTGGAAAGGCGACTAACTCGTCATTCCAAGGAACACTGCCATGCGCTCCACCGTTGGCTAccaca                                                                                                                                                                                          >  2:682077/1‑297 (MQ=255)                                                                                                                           gcgcAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTgg                                                                                                                                                                           <  1:668458/301‑1 (MQ=255)                                                                                                                                  catacccgcTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCct                                                                                                                                                                    <  2:50164/293‑1 (MQ=255)                                                                                                                                                cTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGTCCAGccccc                                                                                                                                                      >  2:103344/1‑300 (MQ=255)                                                                                                                                                             gTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTCGCCGACAg                                                                                                                                         >  1:753382/1‑301 (MQ=255)                                                                                                                                                                            gCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCtt                                                                                                                          >  1:195175/1‑301 (MQ=255)                                                                                                                                                                                        tttttttCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTGCATCGACGGCAc                                                                                                              >  2:527700/1‑301 (MQ=255)                                                                                                                                                                                         ttttttCGACCTGCCGAAAGCGCGGTGGGGCCGGCAAAAGCAGTTGTGCAGATTGCTGCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCAcg                                                                                                             <  2:279488/301‑1 (MQ=255)                                                                                                                                                                                            tttCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCgg                                                                                                          <  1:584222/301‑1 (MQ=255)                                                                                                                                                                                             ttCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCggg                                                                                                         <  1:309409/301‑1 (MQ=255)                                                                                                                                                                                                     gACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTCGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCAtgtg                                                                                                 <  1:138494/301‑1 (MQ=255)                                                                                                                                                                                                       gggaggggcTGTGGCGCCGGCAACAGCAGTTGTGCAGGTTGCTTCGCAGACGGGGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCt                                                                                               <  1:199440/294‑1 (MQ=255)                                                                                                                                                                                                        gAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTc                                                                                              <  1:725925/301‑1 (MQ=255)                                                                                                                                                                                                                               agcagTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAATTTCTTCTTCAGCCa                                                                       <  1:294697/301‑1 (MQ=255)                                                                                                                                                                                                                                          aGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAATTTCTTCTTCAGCCAGGACTATCGCa                                                            >  1:580104/1‑301 (MQ=255)                                                                                                                                                                                                                                            aTTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGTGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAATTTCTTCTTCAGCCAGGACTATCGCAAc                                                          <  2:253660/301‑1 (MQ=255)                                                                                                                                                                                                                                                       gACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAATTTCTTCTTCAGCCAGGACTATCGCAACGGCGACTTTac                                               <  1:527921/301‑1 (MQ=255)                                                                                                                                                                                                                                                           gAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAATTTCTTCTTCAGCCAGGACTATCGCAACGGCGACTTTACACTc                                           >  1:152651/1‑301 (MQ=255)                                                                                                                                                                                                                                                              catcatAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAATTTCTTCTTCAGCCAGGACTATCGCAACGGCGACTTTACACTCAAt                                        <  1:109533/301‑1 (MQ=255)                                                                                                                                                                                                                                                               atcatAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAATTTCTTCTTCAGCCAGGACTATCGCAACGGCGACTTTACACTCAATc                                       <  1:537751/301‑1 (MQ=255)                                                                                                                                                                                                                                                                tcatAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCTACCGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCCTAACCGCAATTTCTTCTTCAGCCAGGACCATCGCAACCGCGACTTTCCACtacttcc                                      >  2:190530/1‑295 (MQ=255)                                                                                                                                                                                                                                                                 catAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAATTTCTTCTTCAGCCAGGACTATCGCAACGGCGACTTTACACTCAATccc                                     >  1:684972/1‑301 (MQ=255)                                                                                                                                                                                                                                                                          gAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAATTTCTTCTTCAGCCAGGACTATCGCAACGGCGACTTTACACTCAATCCCAATAATGGc                            <  1:83522/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                   gttCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCCCTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTATCTCTGTCCATCCTGCTGTCCAGCCCCGTTTTTGCCGACAGCGTTGATGAATGCTTCAGCGCTGGCACGCGGGTCCATATGCCTAACAGCAATTCCTTCTTAAGCCAGGATTATCGCAACGGAGACTTTACACTCAATCCCATTAATTGCGAGCTGCaa                   >  2:485749/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      cAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAATTTCTTCTTCAGCCAGGACTATCGCAACGGCGACTTTACACTCAATCCCACTAATGGCGAGCGGCagtgc                >  1:734780/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                           gCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAATTTCTTCTTCAGCCAGGACTATCGCAACGGCGACTTTACACTCAATCCCAATACTGGCGCGCGGCAGGGCCTGcc           >  2:731562/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                           gCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAATTTCTTCTTCAGCCAGGACTATCGCAACGGCGACTTTACACTCAATCCCAATAATGGCGAGCGGCAGAGCCTGCa           <  1:533668/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                            cTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAATTTCTTCTTCAGCCAGGACTATCGCAACGGCGACTTTACACTCAATCCCAATAATGGCGAGCGGCAGAGCCTGCAt          >  1:664134/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                                  tAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAATTTCTTCTTCAGCCAGGACTATCGCAACGGCGACTTTACACTCAATCCCAATAATGGCGAGCGGCAGAGCCTGCATCGTGaa    >  1:176739/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                                    aCAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAATTTCTTCTTCAGCCAGGACTATCGCAACGGCGACTTTACACTCAATCCCAATAATGGCGAGCGGCAGAGCCTGCATCGTGAATg  <  1:103390/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                       TCCTGGAAAACGGCCGGGTGGTAGGTGAGGGCAGTGCTGCCGAGCTGGCCGCGCGGGAAGATATTCAGCATTTCTACCTGGGCGGCAAGGGCTGACTGAGCGGTGGCGACGGCTGTGGAGCGGCGCAGTCATACCGCCTTTTACTGAGTAGGTGATGTTCCATTCAACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGTTTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGCCATCCTGCTGGCCAGCCCCGCTTTTGCCGACAGCGATGATGAAGGCTTCATCGACGGCACGCGGGTCCATGTGCTCAACCGCAATTTCTTCTTCAGCCAGGACTATCGCAACGGCGACTTTACACTCAATCCCAATAATGGCGAGCGGCAGAGCCTGCATCGTGAATG  >  AE015451/3137478‑3138070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑