Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451	4,740,811	(T)7→6	intergenic (+48/+705)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	4,740,805	0	T	.	100.0%	74.8 / NA	18	intergenic (+42/+711)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (9/9);  total (9/9)

GAAGAGATCGCCCTGACCGATCCTTACTTCATCGAGCGCTCGCTGTACCCGAACGTCGACTTCTACTCGGGGATCATCCTCAAGGCGATCGGCATTCCGACCAGCATGTTCACCGTGATCTTCGCCCTGGCACGTACCGTGGGCTGGATCTCGCACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTT‑TTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGGTGTGCACCTGGTAGGTCACTTCAGTGCTGTCACCCTTGGGCACCAGCTTCCAGAAGCCGTCGACTTTTGCCACCCGAACAAAACCCTTCTCTTCCGGC  >  AE015451/4740506‑4741079                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                    gAAGGGATCACCCTGACCGATCCTTCCTTCATCGAGAGCTCGCTGTACCCGAACGTCGACTTCTACTCGGGGAGCATCCTCAAGGCGATCGGCATTCCGACCAGCATGTTCACCGTGATCTTCGCCCTGGCACGTACCGTGGGCTGGATCTCGCACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCtt                                                                                                                                                                                                                                                                                    <  2:494005/301‑1 (MQ=255)     agaTCGCCCTGACCGATCCTTACTTCATCGAGCGCTCGCTGTACCCGAACGTCGACTTATACTCGGGGATCATCCTCAAGGCGATCGGCATTCCGACCAGCATGTTCACCGTGATCTTCGCCCTGGCACGTACCGTGGGCTGGATCTCGCACTGGAAAGAAATGCTCTCCAGCCGGTACAAGATCGGCCGTCCGAGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCGGCCCTCGGGCAGCCtgtttt                                                                                                                                                                                                                                                                                >  1:32261/1‑301 (MQ=255)        ccGCCCTGACCGATCCTTACTTCATCGAGCGCTCGCTGTACCCGAACGTCGACTTCTACTCGGGGATCATCCTCAAGGCGATCGGCATTCCGACCAGCATGTTCACCGTGATCTTCGCCCTGGCACGTACCGTGGGCTGGATCTCGCACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttttatt                                                                                                                                                                                                                                                                             <  1:516788/300‑4 (MQ=255)                  cgaaccTTACTTCATCGAGCGCTCGCTGTACCCGAACGTCGACTTCTACTCGGGGATCATCCTCAAGGCGATCGGCATTCCGACCAGCATGTTCACCGTGATCTTCGCCCTGGCACGTACCGTGGGCTGGATCTCGCACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttttatttgtcctgtgc                                                                                                                                                                                                                                                                   <  1:2090/297‑14 (MQ=255)                                      gctcgctGTACCCGAACGTCGACTTCTACTCGGGGATCATCCTCAAGGCGATCGGCATTCCGACCAGCATGTTCACCGTGATCTTCGCCCTGGCACGTACCGTGGGCTGGATCTCGCACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGGCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGATGCGGTAGCCGAACGCAAGAAGGCTGCCATCGGGCAGCC‑TTTTTTATTT‑TTCCTGTGCCGGCCTTTTCGCTGCCtttc                                                                                                                                                                                                                                             >  2:260435/1‑299 (MQ=255)                                                    aaCGTCGACTTCTACTCGGGGATCATCCTCAAGGCGATCGGCATTCAGACCAGCATGTTCACCGTGATCTTCGCCCTGGCACGTACCGTGGGCTGGATCTCGCACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTT‑GTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCg                                                                                                                                                                                                                               <  1:319470/301‑1 (MQ=255)                                                                                                                      aTCTTCGCCCTGGCACGTACCGTGGGCTGGATCTCGCACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTT‑GTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCTGCACACACACCACCAAACCTCAGTTTTTCCCCGCCCGCCCACCCAGCCCTTTCAGGGTATTGAAcc                                                                                                                                                             >  2:455058/1‑300 (MQ=255)                                                                                                                      aTCTTCGCCCTGGCACGTACCGTGGGCTGGATCTCGCACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTT‑GTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACg                                                                                                                                                             >  1:742081/1‑301 (MQ=255)                                                                                                                       tCTTCGCCCTGGCACGTACCGTGGGCTGGATCTCGCACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTT‑GTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACgg                                                                                                                                                            <  2:741767/301‑1 (MQ=255)                                                                                                                                      gTACCGTGGGCTGGATCTCGCACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTT‑GTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCCCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACgca                                                                                                                                             >  1:571839/1‑299 (MQ=255)                                                                                                                                             ggaaggaaTCTCGCACTGGAAAGAAATACTCTCCAGCCCGTACAAGATGGGCCGTCCGCGCGAGCTGTACAACGGCGCGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTT‑GTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgttg                                                                                                                                      <  2:40018/294‑1 (MQ=255)                                                                                                                                                                      aTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTT‑GTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCACCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCAAGAACTTGCTGGCCACCATCGCCGGCACGCTacccc                                                                                                             >  1:282272/1‑299 (MQ=255)                                                                                                                                                                               agcacgaaCAAGGTCCGCCGTCCGCGCAAGCTGTACACCGGCGACAAGAAGCGCGACATCGTTGCCAGAAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGGAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTT‑GTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCgg                                                                                                    <  2:571594/294‑1 (MQ=255)                                                                                                                                                                                                         ccAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTT‑GTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGCATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCCCGGTGTGCACCTGGTAGGCCACtcaagtt                                                                          >  1:471870/1‑295 (MQ=255)                                                                                                                                                                                                               gTACATCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTT‑GTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGGTGTGCACCTGGTAGGTCACTTCAGTGCTGTCa                                                                    <  2:691748/301‑1 (MQ=255)                                                                                                                                                                                                                   acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTT‑GTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGGTGTGCACCTGGTAGGTCACTTCAGTGCTGTCAACCt                                                                >  1:192679/1‑301 (MQ=255)                                                                                                                                                                                                                                      aCATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTT‑GTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGGTGTGCACCTGGTAGGTCACTTCAGTGCTGTCACCCTTGGGCACCAGCTTCCAGaa                                             <  2:172742/301‑1 (MQ=255)                                                                                                                                                                                                                                            ttGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTT‑GTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGGTGTGCACCTGGTAGGTCACTTCAGTGCTGTCACCCTTGGGCACCAGCTTCCAGAAGCCGTc                                       <  1:455248/301‑1 (MQ=255)                                                                                                                                                                                                                                                                   gCTGCAGTGGCCGAACGCAAGAAGACTGCTCTCGGGCAGCC‑TTTTTTGTCA‑GTCCTGTCGCGGCATCTTCGCGGCCTTGCCCGCGAAGACGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGGTCACGCAGCCCTTTCAGGGTGTTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTAGCTCGGTGTGCACCTGGTAGGTCACTTCAGTGCTGTCACCCTTGGGCACCAGCTTCCAGAAGCCGTCGACTTTTGCCACCCGAGCaaatc                <  2:30377/301‑3 (MQ=255)                                                                                                                                                                                                                                                                     tGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTT‑GTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCTGTGTGCACCTGGTAGGTCACTTCAGTGCTGTCACCCTGGGGCACCAGCTTCCAGAAGCCGTCGACTTTTGCCACCCGAACAAAcccc              >  1:359134/1‑301 (MQ=255)                                                                                                                                                                                                                                                                           aGCCGAAAGCAAGAAGGCTGGCATCGGGCAGCC‑TTTTTTATTTGTCCTGG‑GCCGGCCTCTACGCGGCCTTGCCCGCGAAGAGGCCGGCACCGCCACCACCAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTAGCCACCATCGCCGGCACGCTACCACCTGGCTCGGTGTGCACCTGGTAGGTCACTTCAGTGCTGTCACCCTTGGGCACCAGCTTCCAGAAGCCGTCGACTTTTGCCACCCGAACAAAACCCTTctct        <  2:32246/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                 aCGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTT‑GTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGGTGTGCACCTGGTAGGTCACTTCAGTGCTGTCACCCTTGGGCACCAGCTTCCAGAAGCCGCCGACTTTTTCCACCCGAACAAACCCCTTCTCTTCCGGc  >  2:701448/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                    GAAGAGATCGCCCTGACCGATCCTTACTTCATCGAGCGCTCGCTGTACCCGAACGTCGACTTCTACTCGGGGATCATCCTCAAGGCGATCGGCATTCCGACCAGCATGTTCACCGTGATCTTCGCCCTGGCACGTACCGTGGGCTGGATCTCGCACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTT‑TTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGGTGTGCACCTGGTAGGTCACTTCAGTGCTGTCACCCTTGGGCACCAGCTTCCAGAAGCCGTCGACTTTTGCCACCCGAACAAAACCCTTCTCTTCCGGC  >  AE015451/4740506‑4741079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑