Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451	4,586,057	+C	intergenic (+140/+75)	PP_4061 → / ← PP_4063	conserved protein of unknown function/putative Long‑chain‑fatty‑acid‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	4,586,056	1	.	C	100.0%	45.4 / NA	14	intergenic (+139/+76)	PP_4061/PP_4063	conserved protein of unknown function/putative Long‑chain‑fatty‑acid‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (7/7);  total (7/7)

GAACATTCCCATTCCACCGGAGACACCCGATCCCAATATCGACGACCCGAGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCAGGGA‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTGCGATGCGTGCCTTGCACCAGCCCTGCAGCTCCTCGACCGTGGCGCTGTGCCCCGGGTGCAGCTTGATCCAGGCGACGATCTCTTCGCCA  >  AE015451/4585758‑4586348                                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                     gAACATTCCCATTCCACCGGAGACACCCGATCCCAATATCGACGACCCGAGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGA‑c                                                                                                                                                                                                                                                                                                     <  1:552470/301‑1 (MQ=255)                   ggAGACACCCGATCCCAATATCGACGACCCGAGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTACCGGCCTTGACTgc                                                                                                                                                                                                                                                                                    >  2:755464/1‑301 (MQ=255)                                cccaaatctccagaccagggctggccgcaACCCGTACCGGAAGAAGACCCGGGCGAGCCGCCGATAAAGCCGACCGTGCCGCGGACAGTAGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCAGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTg                                                                                                                                                                                                                                                                       <  2:407841/278‑1 (MQ=255)                                              cccGAGCTTGCCGCCACCCGGACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGttt                                                                                                                                                                                                                                                         <  2:329273/301‑1 (MQ=255)                                                  cgatctccgccACCCGTCGCGGAAGAAGACCCGGACTGTCTGCCGATCAAGGCTACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTAccc                                                                                                                                                                                                                                                     <  2:38231/295‑1 (MQ=255)                                                        ccgccACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTAcccagatcg                                                                                                                                                                                                                                               >  1:38246/1‑295 (MQ=255)                                                                                                      cGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAAtct                                                                                                                                                                                                 <  1:755780/301‑1 (MQ=255)                                                                                                                                    cgAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCTGCGCCAGCACATCAACCCGCAGATGCAGCCCCCATCTCCGCCACCCTGATCCCACGCATTCGGAAcc                                                                                                                                                                   >  2:62787/1‑300 (MQ=255)                                                                                                                                                   cGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCCACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCCCACGACTGCGGAACTTCTGCACTTTGcctg                                                                                                                                                    >  2:116259/1‑299 (MQ=255)                                                                                                                                                              tAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATc                                                                                                                                         <  1:82778/301‑1 (MQ=255)                                                                                                                                                                                           aGCTGATCTGCGCTGCTCCAGTGACAGGTGGAGGATGACCGGGTGCTTGGCAATGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACcg                                                                                                            <  1:550218/301‑1 (MQ=255)                                                                                                                                                                                                                                       gCTTGGCAAGGTGAAGATCGTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTGCGATGCGTGCCTTGCACCAGCCCTGCa                                                                <  1:62818/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                  cgcgggTAAACCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTGCGATGCGTGCCTTGCACCAGCCCTGCAGCTCCTCGACCGTGGCCCTGTGCCCCGGGTGCAGCTTGATccc                     >  2:458595/5‑300 (MQ=255)                                                                                                                                                                                                                                                                                          aaCCCGCTCCTACCAGGGACCGTGCCGGCCTTGAATGTGGCGCTGCCCCCGGTAGGAGCGGGTTTACTCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTCTGCGATGCGTGCCTTGCACCAGCCCTGCAGCTCCTCGACCGTGCCGCTGTCCCTCTGGTTCAGCTTGATCCAGGcgacgc             >  1:733069/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                     aCCAGGGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTGCGATGCGTGCCTTGCACCAGCCCTGCAGCTCCTCGACCGTGGCGCTGTGCCCCGGGTGCAGCTTGATCCAGGCGACGATCTCTTCGCCa  >  1:187711/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                     GAACATTCCCATTCCACCGGAGACACCCGATCCCAATATCGACGACCCGAGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCAGGGA‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTGCGATGCGTGCCTTGCACCAGCCCTGCAGCTCCTCGACCGTGGCGCTGTGCCCCGGGTGCAGCTTGATCCAGGCGACGATCTCTTCGCCA  >  AE015451/4585758‑4586348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑