Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451	336,125	+T	coding (210/237 nt)	PP_0278 →	conserved protein of unknown function

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	336,124	1	.	T	100.0%	165.6 / NA	48	D70V (GAT→GTT)	PP_0278	conserved protein of unknown function
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (21/27);  total (21/27)

TCGGTTTCTCACCCTGCGTACGGCTGCCGCCCCAAATCCGTGAGAAAGATTTTTGGCAGTCTCCTTCCGTGAATAAGGATTTGCC‑ATGAAAAAAGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑TACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGATTGCGCTGAAAACGGGGGCCGCTTTGCGGCCCTTTCGCGACGCA‑AGGCCGCTCCCACAAAAGCCGCTTGCGCTAACCACAAGAAATGTTTATGCACAAGTGGCATTTGGGACCTGAGCGAAAGGCATGCCGGATCGCTAC  >  AE015451/335831‑336405                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                           tCGGTTTCTCACCCTGCGTACGGCTGCCGCCCCAAATCCGTGAGAAAGATTTTTGGCAGTCTCCTTCCGTGAATAAGGATTTGCC‑ATGAAAAAAGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCtg                                                                                                                                                                                                                                                                                      >  2:111589/1‑301 (MQ=255)       tctcACCCTGCGTACGGCTGCCGCCCCAAATCCGTGAGAAAGATTTTTGGCAGTCTCCTTCCGTGAATAAGGATTTGCC‑ATGAAAAAAGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAACCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGAc                                                                                                                                                                                                                                                                                >  1:448724/1‑301 (MQ=255)         tcctacggactagtggtgccgcagcAAATCCGTGAGAAATAATTTGGGCAGTCTCGTTCGGGGACTAAGGATTTGCC‑ATGAAAAAAGATGTTTCTGGGCCAAACACAACCGCCGGCGTCGAAACATTACTCGCAGCCGTTAACCCATCCCTCGATCTCCTGCGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCAGGTCTAGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACAt                                                                                                                                                                                                                                                                              <  2:607866/277‑1 (MQ=255)         tcACCCTGCGTACGGCGGCCGCCCCAAATCCGTGAGAAAAATTTTTGGCAGTCTCCTTCCGGGAATAAGGATTTGCC‑ATGAAAAAAGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACAt                                                                                                                                                                                                                                                                              <  1:597432/301‑1 (MQ=255)                 tgTACGGCTGCCGCCCCAAATCCGTGGGAAAGATTTTTGGCAGTCTCCTTCCGTGAATAAGGATTTGCC‑ATGAAAAAAGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAgg                                                                                                                                                                                                                                                                      <  2:610055/300‑1 (MQ=255)                 cGTACGGCTGCCGCCCCAAATCCGTGAGAAAGATTTTTGGCAGTCTCCTTCCGTGAATAAGGATTTGCC‑ATGAAAAAAGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAgg                                                                                                                                                                                                                                                                      >  2:524380/1‑301 (MQ=255)                  gTACGGCTGCCGCCCCAAATCCGTGAGAAAGATTTTTGGCAGTCTCCTTCCGTGAATAAGGATTTGCC‑ATGAAAAAAGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAgga                                                                                                                                                                                                                                                                     >  1:610316/1‑300 (MQ=255)                    aCGGCTGCCGCCCCAAATCCGTGAGAAAGATTTTTGGCAGTCTCCTTCCGTGAATAAGGATTTGCC‑ATGAAAAAAGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGgcgc                                                                                                                                                                                                                                                                   >  1:471202/1‑301 (MQ=255)                      ggCTGCCGCCCCAAATCCGTGAGAAAGATTTTTGGCAGTCTCCTTCCGTGAATAAGGATTTGCC‑ATGAAAAAAGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCAAAggggagg                                                                                                                                                                                                                                                                 >  2:178934/1‑296 (MQ=255)                                       cGTGAGAAAGATTTTTGGCAGTCTCCTTCCGTGAATAAGGATTTGCC‑ATGAAAAAAGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGAt                                                                                                                                                                                                                                                <  1:654926/301‑1 (MQ=255)                                           agaAAGATTTTTGGCAGTTTCCTTCCGTGAATAAGGATTTGCC‑ATGAAAAAAGATGTTGCTGAGCCCATCACAACCGCAGGCGTGGAAACCTTCCTAGCCGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGcc                                                                                                                                                                                                                                            <  2:16330/301‑1 (MQ=255)                                                 tttgttGGCAGTATACTTCCGTGAATAAGGATTTGCC‑ATGAAAAAAGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGt                                                                                                                                                                                                                                      <  2:725712/297‑1 (MQ=255)                                                 aTTTTTGGCAGTCTCCTTCCGTGAATAAGGATTTGCC‑ATGAAAAAAGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGt                                                                                                                                                                                                                                      <  1:524601/301‑1 (MQ=255)                                                     ttGGCAGTCTCCTTCCGTGAATAAGGATTTGCC‑ATGAAAAAAGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAgg                                                                                                                                                                                                                                  <  2:471000/301‑1 (MQ=255)                                                                   ccGTGGATAAGGATTTGCCAATG‑GAAAACATGTTCCGCAGCCAATCACGACCGCAGGGGTGGAAGCCTTCCTCGCAGCCGTTAACCCGTCCCTCGCTCGCCTACGCGTGGAGCCCGGCGTCCCCGTCGACGCGGCCTACGGGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGGACGATATCGAGAGCGCCAAGGGTAGGGCGCATTAGGCATTg                                                                                                                                                                                                                    <  2:754471/301‑1 (MQ=255)                                                                   ccGTGAATAAGGATTTGCC‑ATGAAAAAAGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTg                                                                                                                                                                                                                    <  1:111637/301‑1 (MQ=255)                                                                            aGGATTTGCC‑ATGAAAAAAGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTAATGAACGATAACGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCa                                                                                                                                                                                                           >  2:199077/1‑301 (MQ=255)                                                                                  tGCC‑ATGAAAAAAGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgtg                                                                                                                                                                                                     <  1:101389/301‑1 (MQ=255)                                                                                  tGCC‑ATGAAAAAAGATGTCTCTGAGCCAATCACAACCGCAGGCGTGGACACCTTCCTCGCAGCCGTTAACCCAGCCATCAATCGCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCGACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGAGATATGGAGGACGACCATAAATTCCTGGGGGCAGACGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgtg                                                                                                                                                                                                     <  2:588942/301‑1 (MQ=255)                                                                                    cc‑ATGAAAAAATATGTTGCTGCTCCAATCAAAACCGCAGCCCTGCAACCCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTggg                                                                                                                                                                                                   <  2:322570/301‑1 (MQ=255)                                                                                    cc‑ATGAAAAAAGATGTTTCCGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTTCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTGGTggg                                                                                                                                                                                                   >  2:32136/1‑301 (MQ=255)                                                                                     c‑ATGAAAAAAGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGAATTAGGCATTGACGCGGTCATTTGTgggg                                                                                                                                                                                                  >  1:80138/1‑300 (MQ=255)                                                                                         gAAAAAAGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCTCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCg                                                                                                                                                                                               >  1:293502/1‑301 (MQ=255)                                                                                              aaGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCtt                                                                                                                                                                                          <  1:352549/301‑1 (MQ=255)                                                                                                 aTGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCg                                                                                                                                                                                       >  1:144907/1‑301 (MQ=255)                                                                                                   gTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCCAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGTGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGAATTAGGCATTGAGTGGGTCATTTGTggggacgtccttccggc                                                                                                                                                                                     >  2:287119/1‑287 (MQ=255)                                                                                                    tttCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGttg                                                                                                                                                                                    >  1:338877/1‑299 (MQ=255)                                                                                                       cTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGACATCGCCAAGGGTAAGGCGCATTAGGCATTGACGAGGTCATTTGTGGGAGCGGCCTTGCGTCGCGa                                                                                                                                                                                 >  2:369930/1‑301 (MQ=255)                                                                                                        tGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGTCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCTTTTGTGGGACCGGCCTTGCGTCTCGaa                                                                                                                                                                                >  1:348091/1‑301 (MQ=255)                                                                                                           gCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATgg                                                                                                                                                                             <  1:115304/301‑1 (MQ=255)                                                                                                                 cacaACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGACCGGCCTTGCGCCGCGAATGGGCTGcc                                                                                                                                                                       >  2:454607/1‑300 (MQ=255)                                                                                                                       cGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAagcag                                                                                                                                                                 <  1:179008/301‑1 (MQ=255)                                                                                                                                    cccTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTAGGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGGCTGGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGGTATGAAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTc                                                                                                                                                    <  1:32151/300‑1 (MQ=255)                                                                                                                                      cTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCag                                                                                                                                                  <  1:360375/301‑1 (MQ=255)                                                                                                                                      cTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCag                                                                                                                                                  >  1:459205/1‑301 (MQ=255)                                                                                                                                         ccTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAgaga                                                                                                                                               <  1:370078/301‑1 (MQ=255)                                                                                                                                           ccGCAACCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGAtt                                                                                                                                             <  2:448534/300‑1 (MQ=255)                                                                                                                                                 ccGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGGGGCCTGCCGTCGGGAATGGGCTGCAAAGCAGGCCAGGAGATTTCAGGGAtggctgtg                                                                                                                                       >  1:694074/1‑294 (MQ=255)                                                                                                                                                   gTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGATTGCGCTGaa                                                                                                                                     <  1:496084/301‑1 (MQ=255)                                                                                                                                                             cccTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGATTGCGCTGAAAACGGGGGcc                                                                                                                           <  1:454797/301‑1 (MQ=255)                                                                                                                                                             cacccaatccccaagcgcgatggCCCGGCGTCCCCGCAGAAGCGGCCTACGAGCTGGTCTCGATCCTGACGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATAGAAGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGATTGCGCTGAAAACGGGGGcc                                                                                                                           <  2:244814/279‑1 (MQ=255)                                                                                                                                                                    tctcCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGATTGCGCTGAAAACggggcgcgattttg                                                                                                                    >  1:211725/1‑291 (MQ=255)                                                                                                                                                                          aCGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCACCCCCGGCGATTGCAGAGATTGCGCTGAAAACGGGGGCAGCTTTTCGCCCCt                                                                                                              >  2:190062/1‑301 (MQ=255)                                                                                                                                                                                       ggCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGATTGCGCTGAAAACGGGGGCCGCTTTGCGGCCCTTTCGCGACGCA‑Ag                                                                                                <  2:338750/301‑1 (MQ=255)                                                                                                                                                                                                                       ctcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGAGGGCCGCGTGGGCTGGAACGCAAGCCGGGCGGTGTCAGCGATTGCGCTGAAAACCGGTGCCGCGTTGCGTCCCTTTCGCG‑CGGACCGTACGCTCCCACAAAAGCCGCTCGCGCTagtca                                                                >  1:437201/1‑297 (MQ=255)                                                                                                                                                                                                                               tGATGGGCTACATCAAGAACCTGGTGCTCGAGGGCGATATGAAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGAGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGAATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGATTGCGCTGAAAACGGGGGCCGCTTTGCGGCCCTTTCGCGACGCA‑AGGCCGCTCCCACAAAAGCCGCTTGCGCTAACCACAAGAAAt                                                        <  1:727508/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                     acttCCTGGGGCCAGCCGATTACCGGTGCGACATGGCCAAGGCGCTAATGAACGATATCGGGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGATTGCGCTGAAAACGGGGGCCGCTTTGCGGCCCTTTCGCGACGCA‑AGGCCGCTCCCACAAAAGCCGCTTGCGCTAACCACAAGAAATGTTTATGCACAAGTGGCATTTGGGACCTGAGCGAAAGGCATGCCGGATCGCTAc  <  1:199157/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                     aatgccTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGATTGCGCTGAAAACGGGGGCCGCTTTGCGGCCCTTTCGCGACGCA‑AGGCCGCTCCCACAAAAGCCGCTTGCGCTAACCACAAGAAATGTTTATGCACAAGTGGCATTTGGGACCTGAGCGAAAGGCATGCCGGATCGCTAc  <  1:279280/297‑1 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                           TCGGTTTCTCACCCTGCGTACGGCTGCCGCCCCAAATCCGTGAGAAAGATTTTTGGCAGTCTCCTTCCGTGAATAAGGATTTGCC‑ATGAAAAAAGATGTTTCTGAGCCAATCACAACCGCAGGCGTGGAAACCTTCCTCGCAGCCGTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑TACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGATTGCGCTGAAAACGGGGGCCGCTTTGCGGCCCTTTCGCGACGCA‑AGGCCGCTCCCACAAAAGCCGCTTGCGCTAACCACAAGAAATGTTTATGCACAAGTGGCATTTGGGACCTGAGCGAAAGGCATGCCGGATCGCTAC  >  AE015451/335831‑336405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑