Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451 1,124,554 +T coding (589/1122 nt) gcvT‑I ← aminomethyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154511,124,5541.T81.8% 24.2 / 2.2 11coding (589/1122 nt)gcvT‑Iaminomethyltransferase
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base T (9/0);  total (11/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01

GGCAGCAGGCCGACACGTTTGCGCGCAACACCATCGCGCACGTGGGCGAAGATCGCCTCGGCACCCGGGAAGCCGGCAGCACGTGCACCGTCGGCACGGCGCACCTTGGAAATTGCCCACAGCAGGCTGGCTTCGATCGGCGTAGTCTCGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTA‑GCCGTCTTCGCCGGTGTAGCCCGAGCGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAG‑CCAGGCGTTCCAGCACCTTGACTGCAGCCGGGCCTTGCAGGGCGAGCAGGG‑CACGCTCCTCGAACAGCGGCTGCACCTCGCAACGGCTGCCGATGTGGGTTTGCAGGTGAGCCAGGTCCTGCTCCTTGCAGGCGGCGTTGACGACCAGGAACAGGGTGTCCTCGCCCAGGTTGGCAACCATCAGGTCGTCGAGAAT  >  AE015451/1124259‑1124851
                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                           
ggCAGCAGGCCGACACGTTTGCGCGCAACACCATCGCGCACGTGGGCGAAGATCGCCTCGGCACCCGGGAAGCCGGCAGCACGTGCACCGTCGGCACGGCGCACCTTGGAAATTGCCCACAGCAGGCTGGCTTCGATCGGCGTAGTCTCGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTatgccg                                                                                                                                                                                                                                                                                                         >  1:555829/1‑296 (MQ=255)
                                                                                         gTCGGCACGGCGCACCTTGGAAATTGCCCACAGCAGGCTGGCTTCGATCGGCGTAGTCTCGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTATGCCG‑CCTCGCGCGTGTAGCAAGAACGGCTGCCGAAGCAGTCCACACCCAGCCGTTTTACCCGGCGGAACTGCCTGAAGGTCATGCCGGctacc                                                                                                                                                                                                               >  1:280610/1‑297 (MQ=255)
                                                                                                    gcACCTTGGAAATTGCCCACAGCAGGCTGGCTTCGATCGGCGTAGTCTCGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTATGCCGTCTTCTGC‑TTGTAAAAAAAAAAGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGTAACTGCATTAAGGTCATGCCGGCAACtcacggagccag                                                                                                                                                                                                    >  1:459541/1‑290 (MQ=255)
                                                                                                                     cacaGCAGGCTGGCTTCGATCGGCGTAGTCTCGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTATGCCGTCTTCTGC‑TTGAAAAAAAAAAGACTGACGAAGCAGTCCACACCCACCAGTTTTACCCGGCGGCACTGCATGAAGGTCATGCCGGCCAATTCTGAAG‑CCAGGCGTTCCAGCACCTTGa                                                                                                                                                                                  >  1:603906/1‑301 (MQ=255)
                                                                                                                                         cGGCGTAGTCTCGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTATGCCGTCTTCTGC‑TTGAAAAAAAAAAGGCTGACGAAGCAGTGCACACACAGCAGTTTTACACGGCGGAACTGAATGAAGGTCATGCCGGCCACTTCCGGAGACCGGGGGTTCC‑GCGCCATGCCTGCAGCCGGGCCTTgtcgg                                                                                                                                                              >  1:464613/1‑297 (MQ=255)
                                                                                                                                               aGTCTCGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTATGCCGTCTTCTGC‑TTGTAAAAAAAAAGGCTGACGAAGAAGTCCACACCCAGCAGTTTTACCCGGCGGCACTGCATGATGCTCATGCCGGCCACTTCTGCAG‑CCAGGCGTTCCAGCCCCTTTACTGCAGCCGCGCCTTGCCGTGCGAGc                                                                                                                                                        >  1:53609/1‑301 (MQ=255)
                                                                                                                                               aGTCTCGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTATGCCGTCTTCTGC‑GTGAAAACAAAACGGCTGACGAAGCAGGCCACACCCAGCAGTTTTAACCGGCGGAACTGCATGAAGGTCATGCCGGCCACCTCTGGAG‑CCACGCGTTCCAGCACCTCGACTGCAGCCGGGCTTTGCAAGGCGAGc                                                                                                                                                        >  1:676833/1‑301 (MQ=255)
                                                                                                                                                                      cATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTATGCCGTCTTCTGC‑TTGAAAAAAAAAAGGCTGACGAAGCAGTCCACACCCCGCAGTTTTACCCGGCGGAACTCCATCAAGGTCATGCCCGTCACTTCTGGAG‑CCAGGCGGTCCATCACCTTGATCGCCGCCGGGCCTTCCAGGGCGAGCAGGGCCAC‑CTCCTCGAACATCgg                                                                                                                                >  1:419984/1‑301 (MQ=255)
                                                                                                                                                                                                                    cAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTATGCCGTCTTCTGC‑TTGAAAAAAAAAAAGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAG‑CCAGGCGTTCCAGCACCTTGACTGCAGCCGGGCCTTGCATGGCGAGCAGCG‑CAAGCTCCTCGAACAGCTGCTGCACCTCGCAAGTGCTGCCGCTGTTGGTTTGCAGGTTAGGCAgg                                                                                  >  1:639703/1‑301 (MQ=255)
                                                                                                                                                                                                                                     cTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTATGCCGTCTTCTGC‑TTGAAAAAAAAACGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGACGGTCATGCCGGCCACTTCTGGAG‑CCAGGCGTTCCAGCACCTTGACTGCAGCCGGGCCTTGCAGGGCGAGCATGG‑CACGTTCCTCGAACATCGGCTGCACCTCCGAACGGCCGCCGATGTGGGTTTGCAGGTGAGGCAGGGCCTGCTCCtgttagtc                                                                 >  1:11430/1‑294 (MQ=255)
                                                                                                                                                                                                                                                                                                  ctcGTA‑GCCGTCTTCGCCGGTGTAGCCCGAGCGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAG‑CCAGGCGTTCCAGCACCTTGACTGCAGCCGGGCCTTGCAGGGCGAGCAGGG‑CACGCTCCTCGAACAGCGGCTGCACCTCGCAACGGCTGCCGATGTGGGTTTGCAGGTGAGCCAGGTCCTGCTCCTTGCAGGCGGCGTTGACGACCAGGAACAGGGTGTCCTCGCCCAGGTTGGCAACCATCAGGTAGTCgaga    >  1:243139/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                    cGTA‑GCCGTCTTCGCCGGTGTAGCCCGAGCGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAG‑CCAGGCGTTCCAGCACCTTGACTGCAGCCGGGCCTTGCAGGGCGAGCAGGG‑CACGCTCCTCGAACCGCGGCTGCACCTCGAAACGGCTGCCGATGTGGGTTTGCCGGTGAGCCAGGTGCTGCTCCTTTCAGGCGGCGTTGACGACCAGGAACAGGGTGTCCTCGCCCAGGTTGGCAACCATTCGGTCGTCGAGAAt  >  2:557500/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                           
GGCAGCAGGCCGACACGTTTGCGCGCAACACCATCGCGCACGTGGGCGAAGATCGCCTCGGCACCCGGGAAGCCGGCAGCACGTGCACCGTCGGCACGGCGCACCTTGGAAATTGCCCACAGCAGGCTGGCTTCGATCGGCGTAGTCTCGCTGTTCATGTCGTGGCCATACAGGCACAGGCCAGCTTCCAGGCGCAGCGAGTCGCGTGCGCCCAAGCCGATCGGCTGTACTTCCGGCTCGGCCATCAGGCGGCGGGCCAAGGCGTCGGCAGCGTTGACCGGTACCGAGATCTCGTA‑GCCGTCTTCGCCGGTGTAGCCCGAGCGGCTGACGAAGCAGTCCACACCCAGCAGTTTTACCCGGCGGAACTGCATGAAGGTCATGCCGGCCACTTCTGGAG‑CCAGGCGTTCCAGCACCTTGACTGCAGCCGGGCCTTGCAGGGCGAGCAGGG‑CACGCTCCTCGAACAGCGGCTGCACCTCGCAACGGCTGCCGATGTGGGTTTGCAGGTGAGCCAGGTCCTGCTCCTTGCAGGCGGCGTTGACGACCAGGAACAGGGTGTCCTCGCCCAGGTTGGCAACCATCAGGTCGTCGAGAAT  >  AE015451/1124259‑1124851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑