| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 2,038,351 | 0 | G | . | 29.4% | 37.2 / 15.2 | 17 | coding (1129/1143 nt) | rffE | UDP‑N‑acetylglucosamine‑2‑epimerase |
| Reads supporting (aligned to +/- strand): ref base G (12/0); new base . (5/0); total (17/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.54e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACCCTGTGACGTTATCCCTGAGGCATTTCCCCGACAATTCAGCTGCTTCTGACTGGCGCGTAGGATAGTTCGCTTGGTTGTCCGAGTTGAAGAGGTGGGGCGGGGTTTCAGGAAAGTCTGACGTGCGGAATGTGCATTCCAAGGCAAATCTCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGCGGTGGGGGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGC‑GCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCG‑ATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATGAGG > AE015451/2038072‑2038643 | aCCCTGTGACGTTATCCCTGAGGCATTTCCCCGACAATTCAGCTGCTTCTGACTGGCGCGTAGGATAGTTCGCTTGGTTGTCCGAGTTGAAGAGGTGGGGCGGGGTTTCAGGAAAGTCTGACGTGCGGAATGTGCATTCCAAGGCAAATCTCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGCGGTGGGGGGGGGGCCAGGTGCCCAg > 1:692756/1‑301 (MQ=255) tgACGTTATCCCTGAGGCATTTCCCCGACAATTCAGCTGCTTCTGACTGGCGCGTAGGATAGTTTGCTTGGTTGTCCGAGTTGAAGAGGTGGGGCGGGGTTTCAGGAAAGTCTGACGTGCGGTATGTGCATTCCAAGGCAAATCTCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGCGGGGCTGCTGGCCAGTTTTTACGGGGGGGCGGCGGGGCTGCGGAGATCGTATTCGCGGGTGAACCCGCACCTCCATGGAGCGGTgagatcggaagagcacacacctgaactc > 1:364222/1‑274 (MQ=255) gACAATTCAGCTGCTTCTGACTGGCGCGTAGGATAGTTCGCTTGGTTGTCCGAGTTGAAGAGGTGGGGCGGGGTTTCAGGAAAGTCTGACGTGCGGAATGTGCATTCCAAGGCAAATCTCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGCACCCGCACCTACATTGAGCGGTGGGGGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGC‑TCGCGCAGGCTTTGCCAt > 1:352570/1‑301 (MQ=255) tgctTCTGACTGGCGCGTAGGATAGTTCGCTTGGTTGTCCGAGTTGAAGAGGTGGGGCGGGGTTTCAGGAAAGTCTGACGTGCGGAATGTGCATTCCAAGGCAAATCTCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGCGGTGGGGGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGC‑GCGCGCAGGCTTTGCCATCGCCATAgggg > 1:98172/1‑301 (MQ=255) tAGGATAGTTCGCTTGGTTGTCCGAGTTGAAGAGGTGGGGCGGGGTTTCAGGAAAGTCTGACGTGCGGAATGTGCATTCCAAGGCAAATCTCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGCGGTGGGGGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGC‑GCGCGCAGGCTTTGCCATCGCCATCGGGGTTATGGGCGAAGCTCAt > 1:383935/1‑301 (MQ=255) aGTTCGCTTGGTTGTCCGAGTTGAAGAGGTGGGGCGGGGTTTCAGGAAAGTCTGACGTGCGGAATGTGCATTCCAAGGCAAATCTCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGCGGT‑GGGGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGC‑GCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAATCTCATTTCGCG‑a > 1:370820/1‑301 (MQ=255) ggAATGTGCATTCCAAGGCAAATCTCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGCGGT‑GGGGGGGGGCCAGGTGGCCAAGGCTTTTCGTTAGCTGGGCGC‑GGGTTTTCCCTCGGCAACAGGGTTGTTGGCCGAGCGCATTTAGCGAATACG‑CGCCTCATTGGGCCGCAAGGTAATCAACGACCGCACAATCGATGCTACTTAGGTg > 1:493020/1‑301 (MQ=255) aaTGTGCATTCCAAGGCAAATCTCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGCGGTGGGGGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGC‑GCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCG‑ATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATCCTACTTCGGTGc > 1:440908/1‑301 (MQ=255) aTCTCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGCGGTGGGGGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGC‑GCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCG‑ATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTAcc > 1:468505/1‑301 (MQ=255) gttgttGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGCGGTGGGGGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGC‑GCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCG‑ATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGgcgccc > 1:12971/1‑299 (MQ=255) ctgctgGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGCGGT‑GGGGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGC‑GCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCG‑ATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCCTCCACAGCACCGGtt > 1:357686/1‑301 (MQ=255) ggggCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGCGGT‑GGGGGGGGGCCAGGTGGCCAGGGCTTTTCGTATGC‑GCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGCCGAAGCTCATTTCGGG‑ATACGCCGCCTCAACGGTCAGCAAGGTACTCAACGCCCGACCAAACGATGCTACTTCGGTGCCCACCAGCTTGAACGTACATGTCGCCACGGCCTCTGGGCGCTCGGTAGTGTCGTCCATCACCAGCACCGGTTGGCCCGTGGCAGGCGCTCCCTCCTGaagg > 1:122033/1‑299 (MQ=255) tGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGCGGTGGGGGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGC‑GCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCG‑ATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAgagatcggaagagcacacgtctgaact > 1:100449/1‑275 (MQ=255) cGGATATCGTATTCTCGGGTGAACCCGCACCTACATTGAGCGGTGGGGGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGC‑GCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCG‑ATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTCACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCCCCGGTGTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCAccc > 1:733383/1‑301 (MQ=255) gATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGCGGTGGGGGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGC‑GCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCG‑ATACGCCGCCTCATCGGTCAGCAAGGTCCTCAACGCACGCACAATCGATGCTACTTCGGTTCCCACCAGCTTGCCCGTCCCTGCCGCCCCGGCTTCTGGCCGCTCGGTCGTTTCACGCATCCCCATCACCAGTTTTCACCGGCCCGGAGCTTCCGCCTGACTGCCACCCGa > 1:83094/1‑301 (MQ=255) ttCGCGGGTGAACCCGCACCTACATTGAGCGGTGGGGGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGC‑GCGCCCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCG‑ATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCaa > 1:528699/1‑300 (MQ=255) cgGGTGAACCCGCACCTACATTGAGCGGT‑GGGGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGC‑GCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCG‑ATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATcc > 1:337378/1‑300 (MQ=255) tGAGCGGTGGGGGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGC‑GCGCGCAGGCTTTGCCATCGCCATAGGGGTTCTGGGCGAAGCTCATTTCGCG‑ATACGCCGCCTCATCGCTCAGCCAGGTATTCACCGCCCGCACAATCGATGCTACTTCGGTGCACACCAGCTTGACCGTACCTGCCACCACGGCTTCTGTGCGGCCGGTAGTGTCGCGCATCACCAGCACGGGTTTGCCCAGGGCCGTCTCTTCCTCCTGTATCCCACCCGACTCGTTCACAATCACAtcgtcctggctcaccagg > 2:717893/1‑284 (MQ=255) | ACCCTGTGACGTTATCCCTGAGGCATTTCCCCGACAATTCAGCTGCTTCTGACTGGCGCGTAGGATAGTTCGCTTGGTTGTCCGAGTTGAAGAGGTGGGGCGGGGTTTCAGGAAAGTCTGACGTGCGGAATGTGCATTCCAAGGCAAATCTCTTCGTCGTGATTGTTGACTGTTGTTGGGGGGGCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGCGGTGGGGGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGC‑GCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCG‑ATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCACAATCGATGCTACTTCGGTGCCCACCAGCTTGACCGTACCTGCCGCCACGGCTTCTGGGCGCTCGGTAGTGTCGCGCATCACCAGCACCGGTTTGCCCAGGGCCGGCGCTTCCTCCTGAATGCCACCCGAGTCGGTCAGAATCACATAGGCCCGGGTCATGAGG > AE015451/2038072‑2038643 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |